title: Adipose derived mesenchymal stem cells efficiently rescue carbon tetrachloride-induced acute liver failure in mouse.
abstract: OBJECTIVE
  Adipose derived mesenchymal stem cells (ADMSCs) may be an attractive source for acute and chronic liver injury because they are abundant and easy to obtain. We aim to investigate the efficacy of ADMSCs transplantation in the acute liver failure (ALF) caused by carbon tetrachloride (CCl4) in mice.
  
  
  METHODS
  ADMSCs were isolated from inguinal fat pads of enhanced green fluorescent protein (EGFP) transgenic mice and their surface markers and differentiation potential were analyzed. ALF models were established by infusion of CCl4 and divided into two groups: control group; EGFP-ADMSCs transplantation group. The restoration of biological functions of the livers receiving transplantation was assessed via a variety of approaches such as survival rates, live function parameters, histological localization of EGFP-ADMSCs, and Immunofluorescence analysis.
  
  
  RESULTS
  ADMSCs were positive for CD105, CD44 but negative for CD45, CD34 and had adipogenic, osteogenic differentiation potential. The survival rate of transplantation group significantly increased compared to PBS group. Furthermore, the transplanted cells were well integrated into injured livers and produced albumin, cytokeratin-18.
  
  
  CONCLUSIONS
  Direct transplantation of ADMSCs is an effective treatment for ALF. The transplanted ADMSCs exhibit the potential to differentiate into hepatocyte-like cells in the injured livers.
authors.affiliation: Center of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, China ; Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Lisi
authors.initials: L
authors.lastname: Deng
authors.affiliation: Center of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, China.
authors.forename: Guangze
authors.initials: G
authors.lastname: Liu
authors.affiliation: Center of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, China ; Institute of Life Science and Bio-Pharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Guangzhou Eight People's Hospital, Guangdong 510060, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Center of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Tong
authors.affiliation: Center of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Center of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, China.
authors.forename: Kuidong
authors.initials: K
authors.lastname: Wang
authors.affiliation: Center of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, China ; Institute of Life Science and Bio-Pharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Center of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, China.
authors.forename: Xiangping
authors.initials: X
authors.lastname: Kong
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D050154:Adiposity; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002478:Cells, Cultured; D018960:Hyaluronan Receptors / Q000378:metabolism; D039663:Integrin beta4 / Q000378:metabolism; D053538:Keratin-18 / Q000378:metabolism; D017114:Liver Failure, Acute / Q000139:chemically induced* / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
chemical_list: D018960:Hyaluronan Receptors; D039663:Integrin beta4; D053538:Keratin-18; D002251:Carbon Tetrachloride