title: Tracking (Poly)phenol components from raspberries in ileal fluid.
abstract: The (poly)phenols in ileal fluid after ingestion of raspberries were analyzed by targeted and nontargeted LC-MS(n) approaches. Targeted approaches identified major anthocyanin and ellagitannin components at varying recoveries and with considerable interindividual variation. Nontargeted LC-MS(n) analysis using an orbitrap mass spectrometer gave exact mass MS data which were sifted using a software program to select peaks that changed significantly after supplementation. This method confirmed the recovery of the targeted components but also identified novel raspberry-specific metabolites. Some components (including ellagitannin and previously unidentified proanthocyanidin derivatives) may have arisen from raspberry seeds that survived intact in ileal samples. Other components include potential breakdown products of anthocyanins, unidentified components, and phenolic metabolites formed either in the gut epithelia or after absorption into the circulatory system and efflux back into the gut lumen. The possible physiological roles of the ileal metabolites in the large bowel are discussed.
authors.affiliation: Enhancing Crop Productivity and Utilisation Theme, Environmental and Biochemical Sciences Group, The James Hutton Institute , Invergowrie, Dundee DD2 5DA, Scotland.
authors.forename: Gordon J
authors.initials: GJ
authors.lastname: McDougall
authors.forename: Sean
authors.initials: S
authors.lastname: Conner
authors.forename: Gema
authors.initials: G
authors.lastname: Pereira-Caro
authors.forename: Rocio
authors.initials: R
authors.lastname: Gonzalez-Barrio
authors.forename: Emma M
authors.initials: EM
authors.lastname: Brown
authors.forename: Susan
authors.initials: S
authors.lastname: Verrall
authors.forename: Derek
authors.initials: D
authors.lastname: Stewart
authors.forename: Tanya
authors.initials: T
authors.lastname: Moffet
authors.forename: Maria
authors.initials: M
authors.lastname: Ibars
authors.forename: Roger
authors.initials: R
authors.lastname: Lawther
authors.forename: Gloria
authors.initials: G
authors.lastname: O'Connor
authors.forename: Ian
authors.initials: I
authors.lastname: Rowland
authors.forename: Alan
authors.initials: A
authors.lastname: Crozier
authors.forename: Chris I R
authors.initials: CI
authors.lastname: Gill
mesh_terms: D000328:Adult; D000872:Anthocyanins / Q000032:analysis / Q000493:pharmacokinetics; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D047348:Hydrolyzable Tannins / Q000032:analysis / Q000493:pharmacokinetics; D007082:Ileum / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010936:Plant Extracts / Q000032:analysis / Q000493:pharmacokinetics; D059808:Polyphenols / Q000032:analysis* / Q000493:pharmacokinetics; D065856:Rubus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
chemical_list: D000872:Anthocyanins; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; D059808:Polyphenols; C013515:ellagitannin