title: Modified method combining in situ detoxification with simultaneous saccharification and cofermentation (SSCF) as a single step for converting exploded rice straw into ethanol.
abstract: This paper reports an improved fermentation process that includes simultaneous saccharification, detoxification, and cofermentation as steps for producing bioethanol. Rice straw was first steam exploded (SE) or butanone solution exploded (BSE) and then cofermented with Saccharomyces cerevisiae and Candida shehatae. To overcome the inhibitors, the exploded rice straw was continuously and slowly introduced into a 10 L ventilated fermenter. When the fermentation conditions were set to 1.0% initial dry matter, 10% total dry matter, addition rate of 120 mg/min, total fermentation time of 234 h, and dose of 0.1% (NH4)2SO4, yields of 25.8 g/100 g of dry matter ethanol and 88% total sugar use were obtained for BSE rice straw. The ethanol yields were not significantly different between detoxified materials and non-detoxified materials. Most of the furfural, hydroxymethylfurfural (5-HMF), acetic acid, and butanone were removed during the fermentation of non-detoxified materials, and the sugar concentrations were very low. The in situ detoxification and fermentation was effective and inexpensive when the pre-detoxification of exploded materials and the pre-adaptation of strains steps were omitted.
authors.affiliation: Key Laboratory of Fermentation Resource and Application of Institutes of Higher Learning in Sichuan/Department of Life Science and Food Engineering, Yibin University , Yibin, Sichuan 644005, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Wen-xue
authors.initials: WX
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: You
authors.forename: Li-guo
authors.initials: LG
authors.lastname: Yin
authors.forename: Yong-hua
authors.initials: YH
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
mesh_terms: D019342:Acetic Acid / Q000302:isolation & purification; D056804:Biofuels; D019149:Bioreactors; D002074:Butanones / Q000302:isolation & purification; D002175:Candida / Q000378:metabolism; D002241:Carbohydrates / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry*; D005285:Fermentation; D005524:Food Technology; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000302:isolation & purification; D012275:Oryza / Q000737:chemistry* / Q000382:microbiology; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D056804:Biofuels; D002074:Butanones; D002241:Carbohydrates; D000431:Ethanol; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; D019342:Acetic Acid