title: Effects of Lactobacillus rhamnosus GG on hepatic and serum lipid profiles in zebrafish exposed to ethanol.
abstract: Zebrafish is a powerful tool in pharmacological research and useful to identify new therapies. Probiotics can offer therapeutic options in alcoholic liver disease. This study was done in two independent experiments: first, we confirmed the intestinal colonization of probiotic Lactobacillus rhamnosus GG (LGG) after ethanol exposure. Second, four groups were performed: control (C), probiotic (P), ethanol (E), and probiotic+ethanol (P+E). Liver histology, hepatocytes morphometry, hepatic and serum lipid quantifications were conducted in second experiment. During 4 weeks, P and P+E groups were fed with LGG supplemented feed; E and C unsupplemented. E and P+E groups received 0.5% of ethanol added into tank water. Zebrafish exposed to ethanol (E group) presented intense liver steatosis after 28 days in contrast to the almost normalized liver histology of P+E group at the same period. Liver morphometry showed a significant enlargement of hepatocytes of E group after 4 weeks (p<0.0001). Serum triglycerides decreased in P+E group compared with C, P (p<0.001), and E (p=0.004), after 14 and 28 days similarly. Serum cholesterol was also decreased by LGG; P group decreased compared with C and E after 14 days (p=0.002 and p=0.007, respectively) and P+E group decreased significantly compared with E and C groups (p<0.0001) after 28 days. Hepatic triglycerides were reduced in P+E group after 28 days compared to E (p=0.006). The persistence of LGG in zebrafish intestines was demonstrated. LGG decreased serum levels of triglycerides and cholesterol and improved hepatic steatosis.
authors.affiliation: 1 Programa de Pós-Graduação: Ciências em Gastroenterologia e Hepatologia, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
authors.forename: Ana Claudia Reis
authors.initials: AC
authors.lastname: Schneider
authors.forename: Alice Beatriz Mombach Pinheiro
authors.initials: AB
authors.lastname: Machado
authors.forename: Adriano Martimbianco
authors.initials: AM
authors.lastname: de Assis
authors.forename: Djuli Milene
authors.initials: DM
authors.lastname: Hermes
authors.forename: Pedro Guilherme
authors.initials: PG
authors.lastname: Schaefer
authors.forename: Ranieli
authors.initials: R
authors.lastname: Guizzo
authors.forename: Laísa Beduschi
authors.initials: LB
authors.lastname: Fracasso
authors.forename: Fernanda
authors.initials: F
authors.lastname: de-Paris
authors.forename: Fábio
authors.initials: F
authors.lastname: Meurer
authors.forename: Afonso Luis
authors.initials: AL
authors.lastname: Barth
authors.forename: Themis Reverbel
authors.initials: TR
authors.lastname: da Silveira
mesh_terms: D000818:Animals; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D007422:Intestines / Q000382:microbiology; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects; D008297:Male; D019936:Probiotics / Q000378:metabolism*; D015027:Zebrafish / Q000097:blood / Q000378:metabolism / Q000382:microbiology*
chemical_list: D008055:Lipids; D000431:Ethanol