title: Cell division orientation on biospecific peptide gradients.
abstract: An assay was developed for determining cell division orientation on gradients. The methodology is based on permeating microfluidic devices with alkanethiols and subsequent printing of cell adhesive peptide gradient self-assembled monolayers (SAMs) for examining oriented cell divisions. To our knowledge, there has been no study examining the correlation between cell division orientations based on an underlying ligand gradient. These results implicate an important role for how the extracellular matrix may control cell division. These surfaces would allow for a range of cell behavior (polarization, migration, division, differentiation) studies on tailored biospecific gradients and as a potential biotechnological platform to assess small molecule perturbations of cell function.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599, United States.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Lamb
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Sarbajeet
authors.initials: S
authors.lastname: Nagdas
authors.forename: Muhammad N
authors.initials: MN
authors.lastname: Yousaf
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002465:Cell Movement; D016764:Cell Polarity; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D051381:Rats
chemical_list: D010455:Peptides