title: Enhanced saccharification of sugarcane bagasse using soluble cellulase supplemented with immobilized β-glucosidase.
abstract: The β-glucosidase (BG) enzyme plays a vital role in the hydrolysis of lignocellulosic biomass. Supplementation of the hydrolysis reaction medium with BG can reduce inhibitory effects, leading to greater conversion. In addition, the inclusion of immobilized BG can be a useful way of increasing enzyme stability and recyclability. BG was adsorbed on polyacrylic resin activated by carboxyl groups (BG-PC) and covalently attached to glyoxyl-agarose (BG-GA). BG-PC exhibited similar behavior to soluble BG in the hydrolysis of cellobiose, while BG-GA hydrolyzed the same substrate at a lower rate. However, the thermal stability of BG-GA was higher than that of free BG. Hydrolysis of pretreated sugarcane bagasse catalyzed by soluble cellulase supplemented with immobilized BG improved the conversion by up to 40% after 96 h of reaction. Both derivatives remained stable up to the third cycle and losses of activity were less than 50% after five cycles.
authors.affiliation: Department of Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905 São Carlos, São Paulo, Brazil.
authors.forename: Diogo Gontijo
authors.initials: DG
authors.lastname: Borges
authors.affiliation: Department of Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905 São Carlos, São Paulo, Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: Baraldo
authors.affiliation: Department of Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905 São Carlos, São Paulo, Brazil; Embrapa Instrumentação, Rua XV de Novembro 1452, 13560-970 São Carlos, São Paulo, Brazil.
authors.forename: Cristiane Sanchez
authors.initials: CS
authors.lastname: Farinas
authors.affiliation: Department of Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905 São Carlos, São Paulo, Brazil.
authors.forename: Raquel de Lima Camargo
authors.initials: Rde L
authors.lastname: Giordano
authors.affiliation: Department of Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905 São Carlos, São Paulo, Brazil. Electronic address: pwtardioli@ufscar.br.
authors.forename: Paulo Waldir
authors.initials: PW
authors.lastname: Tardioli
mesh_terms: D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002475:Cellobiose; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006038:Glyoxylates / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D031786:Saccharum / Q000378:metabolism*; D012685:Sepharose / Q000737:chemistry; D012995:Solubility; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D006038:Glyoxylates; C070399:glyoxyl agarose; D002475:Cellobiose; D002482:Cellulose; C027433:bagasse; D012685:Sepharose; D001617:beta-Glucosidase; D002480:Cellulase
keywords: Enzyme immobilization; Hydrolysis; Sugarcane bagasse; β-Glucosidase