title: Effects of human fibronectin and human serum albumin sequential adsorption on preosteoblastic cell adhesion.
abstract: Fibronectin (Fn) is widely reported to promote cell adhesion and spreading, and recent reports attest to the synergistic effect of coadsorbed albumin (unexpected due to the passivating character of the latter protein). In this study, the sequential adsorption of fibronectin and albumin, and the morphology of cultured MC3T3-E1 preosteoblastic cells are investigated on three important biomaterial surfaces: silicon oxide, poly(styrene) (PS), and hydroxyapatite (HA). Using quartz crystal microgravimetry with dissipation analysis, the adsorbed protein composition and mechanics are determined. Interestingly, cell morphological changes correlate neither with the amount of Fn nor the rigidity of the protein layer. On the PS surface, Alb is seen to significantly diminish cell spreading, possibly due to Alb aggregation with a partially denatured initially placed Fn layer. HA appears to be a particularly favorable substrate for osteoblast adhesion, despite having low Fn adsorption and protein layer rigidity.
authors.affiliation: ERRMECe, I-MAT, Université de Cergy-Pontoise, 2 Avenue Adolphe Chauvin, 95302 Cergy-Pontoise Cedex, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Hindié
authors.affiliation: ERRMECe, I-MAT, Université de Cergy-Pontoise, 2 Avenue Adolphe Chauvin, 95302 Cergy-Pontoise Cedex, France.
authors.forename: Emeline
authors.initials: E
authors.lastname: Camand
authors.affiliation: ERRMECe, I-MAT, Université de Cergy-Pontoise, 2 Avenue Adolphe Chauvin, 95302 Cergy-Pontoise Cedex, France.
authors.forename: Rémy
authors.initials: R
authors.lastname: Agniel
authors.affiliation: ERRMECe, I-MAT, Université de Cergy-Pontoise, 2 Avenue Adolphe Chauvin, 95302 Cergy-Pontoise Cedex, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Carreiras
authors.affiliation: ERRMECe, I-MAT, Université de Cergy-Pontoise, 2 Avenue Adolphe Chauvin, 95302 Cergy-Pontoise Cedex, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Pauthe
authors.affiliation: Department of Chemical and Environmental Engineering, Yale University, New Haven, Connecticut 06520-8286.
authors.forename: Paul
authors.initials: P
authors.lastname: Van Tassel
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011137:Polystyrenes / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D012709:Serum Albumin / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D011137:Polystyrenes; D012709:Serum Albumin; D012822:Silicon Dioxide; D017886:Durapatite