title: Integrated autofluorescence characterization of a modified-diet liver model with accumulation of lipids and oxidative stress.
abstract: Oxidative stress in fatty livers is mainly generated by impaired mitochondrial β-oxidation, inducing tissue damages and disease progression. Under suitable excitation, light liver endogenous fluorophores can give rise to autofluorescence (AF) emission, the properties of which depend on the organ morphofunctional state. In this work, we characterized the AF properties of a rat liver model of lipid accumulation and oxidative stress, induced by a 1-9-week hypercaloric methionine-choline deficient (MCD) diet administration. The AF analysis (excitation at 366 nm) was performed in vivo, via fiber optic probe, or ex vivo. The contribution of endogenous fluorophores involved in redox reactions and in tissue organization was estimated through spectral curve fitting analysis, and AF results were validated by means of different histochemical and biochemical assays (lipids, collagen, vitamin A, ROS, peroxidised proteins, and lipid peroxidation -TBARS-, GSH, and ATP). In comparison with the control, AF spectra changes found already at 1 week of MCD diet reflect alterations both in tissue composition and organization (proteins, lipopigments, and vitamin A) and in oxidoreductive pathway engagement (NAD(P)H, flavins), with a subsequent attempt to recover redox homeostasis. These data confirm the AF analysis potential to provide a comprehensive diagnostic information on negative effects of oxidative metabolism alteration.
authors.affiliation: IGM-CNR, Via Abbiategrasso 207, 27100 Pavia, Italy ; Department of Biology & Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.
authors.forename: Anna Cleta
authors.initials: AC
authors.lastname: Croce
authors.affiliation: Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.
authors.forename: Andrea
authors.identifier: 0000-0002-9176-4983
authors.initials: A
authors.lastname: Ferrigno
authors.affiliation: IGM-CNR, Via Abbiategrasso 207, 27100 Pavia, Italy.
authors.forename: Valeria Maria
authors.identifier: 0000-0001-9365-3745
authors.initials: VM
authors.lastname: Piccolini
authors.affiliation: Department of Biology & Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Tarantola
authors.affiliation: Department of Biology & Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.
authors.forename: Eleonora
authors.identifier: 0000-0002-6487-5434
authors.initials: E
authors.lastname: Boncompagni
authors.affiliation: Department of Biology & Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.
authors.forename: Vittorio
authors.identifier: 0000-0002-5987-4373
authors.initials: V
authors.lastname: Bertone
authors.affiliation: Department of Biology & Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.
authors.forename: Gloria
authors.identifier: 0000-0002-0454-1416
authors.initials: G
authors.lastname: Milanesi
authors.affiliation: IGM-CNR, Via Abbiategrasso 207, 27100 Pavia, Italy ; Department of Biology & Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.
authors.forename: Isabel
authors.identifier: 0000-0003-4452-137X
authors.initials: I
authors.lastname: Freitas
authors.affiliation: Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.
authors.forename: Mariapia
authors.identifier: 0000-0002-2464-6127
authors.initials: M
authors.lastname: Vairetti
authors.affiliation: IGM-CNR, Via Abbiategrasso 207, 27100 Pavia, Italy ; Department of Biology & Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy.
authors.forename: Giovanni
authors.identifier: 0000-0002-2761-3662
authors.initials: G
authors.lastname: Bottiroli
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004032:Diet; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D018384:Oxidative Stress; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014801:Vitamin A / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D014801:Vitamin A; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D005978:Glutathione