title: Highly selective and sensitive nucleic acid detection based on polysaccharide-functionalized silver nanoparticles.
abstract: Polysaccharide-functionalized silver nanoparticles (Oc-AgNPs) with a mean diameter of 15 nm were utilized as a novel and effective fluorescence-sensing platform for nucleic acid detection. Tests on the oligonucleotide sequences associated with the human immunodeficiency virus as a model system showed that the Oc-AgNPs effectively absorbed and quenched dye-labeled single-stranded DNA through strong hydrogen bonding interactions and slight electrostatic attractive interactions. The proposed system efficiently differentiated between complementary and mismatched nucleic acid sequences with high selectivity and good reproducibility at room temperature.
authors.affiliation: The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China; School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. Electronic address: jkyan_27@163.com.
authors.forename: Jing-Kun
authors.initials: JK
authors.lastname: Yan
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Hai-Le
authors.initials: HL
authors.lastname: Ma
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Pan-Fu
authors.initials: PF
authors.lastname: Cai
authors.affiliation: Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. Electronic address: jian-yong.wu@polyu.edu.hk.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Wu
mesh_terms: D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D006678:HIV / Q000235:genetics*; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000032:analysis*; D011134:Polysaccharides / Q000737:chemistry*; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D055672:Static Electricity
chemical_list: D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D011134:Polysaccharides; D012834:Silver
keywords: Fluorescent sensing platform; Nucleic acid detection; Polysaccharide; Silver nanoparticles