title: First evidence of comparative responses of Toll-like receptor 22 (TLR22) to relatively resistant and susceptible Indian farmed carps to Argulus siamensis infection.
abstract: Toll-like receptor 22 (TLR22) is present in teleost but not in mammals. Among Indian farmed carps, Catla catla is relatively more resistant than Labeo rohita to Argulus siamensis lice infection. TLR22 is believed to be associated with innate immunity against ectoparasite infection. To investigate the TLR22 mediated immunity against argulosis, we have cloned and characterized TLR22 genes of L. rohita (rTLR22) and C. catla (cTLR22). The full-length cDNAs of rTLR22 and cTLR22 contained an open reading frame of 2838 and 2841 nucleotides, respectively; bearing the typical structural features. Phylogenetically rTLR22/cTLR22 was most closely related to Cyprinus carpio (common carp) counterpart, having highest sequence identity of 86.0%. The TIR domain remained highly conserved with 90% identity within freshwater fishes. The sequence information of cDNA and genomic DNA together revealed that the rTLR22/cTLR22 genes are encoded by uninterrupted exons. The co-habitation challenge study with A. siamensis infection confirmed that C. catla is comparatively more resistant than L. rohita. Further, comparative mRNA expression profile in immune relevant tissues also suggested about the participatory role of TLR22 during lice infection. However, TLR22 might not solely be involved in conferring relative resistance among carp species against argulosis.
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (Indian Council of Agricultural Research), Kausalyaganga, Bhubaneswar 751002, Odisha, India.
authors.forename: Rudra Prasanna
authors.initials: RP
authors.lastname: Panda
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (Indian Council of Agricultural Research), Kausalyaganga, Bhubaneswar 751002, Odisha, India.
authors.forename: V
authors.initials: V
authors.lastname: Chakrapani
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (Indian Council of Agricultural Research), Kausalyaganga, Bhubaneswar 751002, Odisha, India.
authors.forename: Swagat Kumar
authors.initials: SK
authors.lastname: Patra
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (Indian Council of Agricultural Research), Kausalyaganga, Bhubaneswar 751002, Odisha, India.
authors.forename: Jatindra Nath
authors.initials: JN
authors.lastname: Saha
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (Indian Council of Agricultural Research), Kausalyaganga, Bhubaneswar 751002, Odisha, India.
authors.forename: Pallipuram
authors.initials: P
authors.lastname: Jayasankar
authors.affiliation: Fish Health Management Division, Central Institute of Freshwater Aquaculture (Indian Council of Agricultural Research), Kausalyaganga, Bhubaneswar 751002, Odisha, India.
authors.forename: Banya
authors.initials: B
authors.lastname: Kar
authors.affiliation: Fish Health Management Division, Central Institute of Freshwater Aquaculture (Indian Council of Agricultural Research), Kausalyaganga, Bhubaneswar 751002, Odisha, India.
authors.forename: Pramoda Kumar
authors.initials: PK
authors.lastname: Sahoo
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture (Indian Council of Agricultural Research), Kausalyaganga, Bhubaneswar 751002, Odisha, India. Electronic address: hkbarman68@hotmail.com.
authors.forename: Hirak Kumar
authors.initials: HK
authors.lastname: Barman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017756:Aquaculture; D033341:Arguloida / Q000502:physiology*; D001483:Base Sequence; D002347:Carps / Q000145:classification / Q000276:immunology* / Q000469:parasitology*; D004478:Ectoparasitic Infestations / Q000276:immunology / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D051193:Toll-Like Receptors / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D029941:Fish Proteins; D051193:Toll-Like Receptors
keywords: Argulus siamensis; Catla catla; Innate immunity; Labeo rohita; Resistance; TLR22