title: Dose-effect of ionizing radiation-induced PIG3 gene expression alteration in human lymphoblastoid AHH-1 cells and human peripheral blood lymphocytes.
abstract: OBJECTIVE
  To identify new ionizing radiation (IR)-sensitive genes and observe the dose-effect of gene expression alteration (GEA) induced by IR.
  
  
  METHODS
  Microarray was used to screen the differentially expressed genes in human lymphoblastoid cells (AHH-1) using three doses of (60)Co γ-rays (0.5-8 Gy at 1 Gy/min). Given that p53-inducible gene 3 (PIG3) was consistently upregulated, the GEA of PIG3 in AHH-1 cells and human peripheral blood lymphocytes (HPBL) induced by γ-rays (1 Gy/min) was measured at messenger RNA (mRNA) and protein levels. The GEA of PIG3 in AHH-1 cells exposed to neutron radiation (californium-252, 0.073 Gy/min) was also quantified.
  
  
  RESULTS
  PIG3 was one of the seven differentially expressed genes found in the microarray analysis. The PIG3 mRNA and protein levels in AHH-1 cells were significantly increased from 1-10 Gy of γ-rays 8-72 h or 8-168 h after exposure, respectively. The enhancement was also observed in AHH-1 cells from 0.4-1.6 Gy of neutrons 48 h post-irradiation. The PIG3 mRNA levels (mRNA copy numbers) in HPBL were significantly increased from 1-8 Gy of γ-rays within 4-24 h post-irradiation, but the highest increase in signal-to-noise responsiveness is approximately two-fold, which was less than that of AHH-1 (approximately 20-fold).
  
  
  CONCLUSIONS
  IR can upregulate the PIG3 gene expression in AHH-1 and HPBL in the early phase after exposure; however, the IR induced expression levels of PIG3 are greater in AHH-1 than HPBL.
authors.affiliation: China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention , Beijing , P. R. China.
authors.forename: Qing-Jie
authors.initials: QJ
authors.lastname: Liu
authors.forename: De-Qin
authors.initials: DQ
authors.lastname: Zhang
authors.forename: Qing-Zhao
authors.initials: QZ
authors.lastname: Zhang
authors.forename: Jiang-Bin
authors.initials: JB
authors.lastname: Feng
authors.forename: Xue
authors.initials: X
authors.lastname: Lu
authors.forename: Xin-Ru
authors.initials: XR
authors.lastname: Wang
authors.forename: Kun-Peng
authors.initials: KP
authors.lastname: Li
authors.forename: De-Qing
authors.initials: DQ
authors.lastname: Chen
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Mu
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
mesh_terms: D000328:Adult; D002460:Cell Line; D003037:Cobalt Radioisotopes; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism* / Q000528:radiation effects*; D008297:Male; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D003037:Cobalt Radioisotopes; D047908:Intracellular Signaling Peptides and Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C411296:TP53I3 protein, human
keywords: Ionizing radiation; dose effect; gene expression; p53-inducible gene 3