title: Inhibition of very long acyl chain sphingolipid synthesis modifies membrane dynamics during plant cytokinesis.
abstract: Plant cytokinesis requires intense membrane trafficking and remodeling to form a specific membrane structure, the cell plate that will ultimately separate the daughter cells. The nature and the role of lipids involved in the formation of the cell plate remain unclear. Plant membranes are particularly rich in sphingolipids such as glucosyl-ceramides with long (16 carbons) or very long (24 carbons) acyl chains. We reveal here that inhibition of the synthesis of sphingolipids with very long acyl chains induces defective cell plates with persistent vesicular structures and large gaps. Golgi-derived vesicles carrying material toward the cell plate display longer vesicle-vesicle contact time and their cargos accumulate at the cell plate, suggesting membrane fusion and/or recycling defects. In vitro fusion experiments between artificial vesicles show that glycosphingolipids with very long acyl chains stimulate lipid bilayer fusion. Therefore we propose that the very long acyl chains of sphingolipids are essential structural determinants for vesicle dynamics and membrane fusion during cytokinesis.
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; Institut Jacques Monod, UMR 7592, CNRS, Université Paris Diderot, Sorbonne Paris Cité, F-75205 Paris, France; Membrane Traffic in Neuronal and Epithelial Morphogenesis, INSERM ERL U950, 75013 Paris, France.
authors.forename: Diana
authors.initials: D
authors.lastname: Molino
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Van der Giessen
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Gissot
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Kian
authors.initials: K
authors.lastname: Hématy
authors.affiliation: Institut des Sciences du Végétal, CNRS, Avenue de la Terrasse, F-91198 Gif-sur-Yvette Cedex, France.
authors.forename: Jessica
authors.initials: J
authors.lastname: Marion
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Barthelemy
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Bellec
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France.
authors.forename: Samantha
authors.initials: S
authors.lastname: Vernhettes
authors.affiliation: Institut des Sciences du Végétal, CNRS, Avenue de la Terrasse, F-91198 Gif-sur-Yvette Cedex, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Satiat-Jeunemaître
authors.affiliation: Institut Jacques Monod, UMR 7592, CNRS, Université Paris Diderot, Sorbonne Paris Cité, F-75205 Paris, France; Membrane Traffic in Neuronal and Epithelial Morphogenesis, INSERM ERL U950, 75013 Paris, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Galli
authors.affiliation: Institut Jacques Monod, UMR 7592, CNRS, Université Paris Diderot, Sorbonne Paris Cité, F-75205 Paris, France; Membrane Traffic in Neuronal and Epithelial Morphogenesis, INSERM ERL U950, 75013 Paris, France.
authors.forename: David
authors.initials: D
authors.lastname: Tareste
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France; AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France. Electronic address: faure@versailles.inra.fr.
authors.forename: Jean Denis
authors.initials: JD
authors.lastname: Faure
keywords: Cell plate; Fatty acyl chain length; Membrane trafficking; Sphingolipid; Vesicle fusion