title: Decrease expression of microRNA-744 promotes cell proliferation by targeting c-Myc in human hepatocellular carcinoma.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are a large group of post-transcriptional gene regulators that potentially play a critical role in tumorigenesis. Increasing evidences indicate that miR-744 deregulated in numerous human cancers including hepatocellular carcinoma (HCC). However, its role in HCC carcinogenesis remains poorly defined. In this study, we investigated the roles of miR-744 in tumor growth of HCC.
  
  
  METHODS
  Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was conducted to detect the expression of miR-744 and Immunohistochemistry was performed to detect expression of c-Myc in HCC specimens and adjacent normal tissues. The biological functions of miR-744 were determined by cell proliferation and cell cycle assay. Furthermore, cell lines transfected with miR-744 mimics were analyzed in vitro. Luciferase reporter assays was performed to confirm whether miR-744 regulated the expression of c-Myc.
  
  
  RESULTS
  Our results showed that the expression of miR-744 was frequently down-regulated in both HCC tissues and cells. Furthermore, restoration of miR-744 in HCC cells was statistically correlated with decrease of cell growth and restored G1 accumulation. Luciferase assay and Western blot analysis revealed that c-Myc is a direct target of miR-744. Down-regulation of miR-744 and up-regulation of c-Myc were detected in HCC specimens compared with adjacent normal tissues. Moreover, restoration of miR-744 rescues c-Myc induced HCC proliferation.
  
  
  CONCLUSIONS
  Our data suggest that miR-744 exerts its tumor suppressor function by targeting c-Myc, leading to the inhibition of HCC cell growth. miR-744 may serve as a potentially useful target for the miRNA-based therapies of HCC in the future.
authors.affiliation: Department of General Surgery, Taizhou First People's Hospital, Taizhou, Zhejiang Province 318020, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of General Surgery, Taizhou First People's Hospital, Taizhou, Zhejiang Province 318020, P.R. China.
authors.forename: Ruliang
authors.initials: R
authors.lastname: Ding
authors.affiliation: Department of General Surgery, Taizhou First People's Hospital, Taizhou, Zhejiang Province 318020, P.R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of General Surgery, Taizhou First People's Hospital, Taizhou, Zhejiang Province 318020, P.R. China.
authors.forename: Dongyi
authors.initials: D
authors.lastname: Xing
authors.affiliation: Department of General Surgery, Taizhou First People's Hospital, Taizhou, Zhejiang Province 318020, P.R. China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Hong
authors.affiliation: Department of General Surgery, The Affiliated Huangyan Hospital of Wenzhou Medical University, Taizhou, Zhejiang Province 318020, P.R. China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Taizhou First People's Hospital, Taizhou, Zhejiang Province 318020, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
keywords: Cyclin D1; Hepatocellular carcinoma (HCC); Tumor suppressors; c-Myc; miR-744