title: Altered expression pattern of miR-29a, miR-29b and the target genes in myeloid leukemia.
abstract: OBJECTIVE
  The miR-29 family have been demonstrated acting as vital tumor suppressor in multiple cancers as well as regulators in the adaptive immune system. Little is known about their role in leukemogenesis. The purpose of this study is to analyze the expression pattern of miR-29a/29b and its target genes Mcl-1 (myeloid cell leukemia sequence 1) and B-cell lymphoma 2 (Bcl-2) in myeloid leukemia.
  
  
  METHODS
  Quantitative real-time PCR was used for detecting genes expression level in peripheral blood mononuclear cells (PBMCs) from 10 cases with newly diagnosed, untreated acute myeloid leukemia (AML) and 14 cases with newly diagnosed, untreated chronic myeloid leukemia (CML) in chronic phase, and 14 healthy individual (HI) served as controls. Correlation between the relative expression levels of different genes have been analyzed.
  
  
  RESULTS
  Significant lower expression of miR-29a/29b and higher expression level of two potential target genes Bcl-2 and Mcl-1 were found in PBMCs from AML and CML patients compared with HI group. In addtion, miR-29a expression in AML was significantly lower than that in CML. Moreover, negative correlation between miR-29a/29b and its target genes have been found. While, positive correlation between relative expression level of miR-29a and miR-29b or Bcl-2 and Mcl-1 were presented in the total 38 research objects.
  
  
  CONCLUSIONS
  Down-regulated miR-29a and miR-29b, and accompanying up-regulated Bcl-2 and Mcl-1 are the common feature in myeloid leukemias. These data further support the role for miR-29a/29b dysregulation in myeloid leukemogenesis and the therapeutic promise of regulating miR-29a/29b expression for myeloid leukemia in the future.
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China ; Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Zhenyi
authors.initials: Z
authors.lastname: Jing
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China ; Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of clinical laboratory, First Affiliated Hospital, Jinan University, Guangzhou 510632, China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zha
authors.affiliation: Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China.
authors.forename: Chengwu
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Lijian
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Gengxin
authors.initials: G
authors.lastname: Luo
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China ; Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China.
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li