title: The influence of different restorative materials on secondary caries development in situ.
abstract: OBJECTIVE
  The effect of direct restorative materials on caries lesion formation was investigated with an 8-week in situ study with split-mouth design, testing the hypothesis that no difference in mineral loss next to a restoration would be found between different composite-based-materials and amalgam.
  
  
  METHODS
  Six groups (n=18) of restored dentine samples were prepared using amalgam, a microhybrid, a nanohybrid and a silorane composite. The composites were adhesively bonded with systems with or without an antibacterial monomer (Clearfil-SE-Protect, Clearfil-SE-bond, respectively), except for the silorane group (Silorane-System-Adhesive). Non-restored dentine samples were used as control (primary caries). Samples were inserted into slots, in lower prosthesis especially made for the experiment. Subjects were instructed to dip the lower prosthesis in a sucrose solution 4 times per day. At baseline and 8 weeks, samples were radiographed extra-orally and the integrated mineral loss was calculated. Data were statistically analyzed using multiple linear regression with a multilevel model (p=0.05).
  
  
  RESULTS
  Nine subjects were selected, and only outer lesions were observed. The hypothesis was partially rejected, as the microhybrid composite bonded with the antibacterial system and the nanohybrid composite presented statistically significant lower mineral loss compared to amalgam. Also, no significant differences were seen for these groups compared to control.
  
  
  CONCLUSIONS
  Within the limits of this study, the restorative material may influence outer lesion progression. Amalgam was not found to be related to lower secondary caries progression in dentine compared to composite-based materials after 8 weeks in situ.
  
  
  CONCLUSIONS
  Although patient factors play a major role in caries progression, the restorative material may affect outer secondary lesion progression.
authors.affiliation: Department of Cariology, Endodontology and Pedodontology, Radboud University Medical Center, Philips van Leydenlaan 25, 6525 EX Nijmegen, The Netherlands; Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Gonçalves Chaves 457, 96015-560 Pelotas, RS, Brazil. Electronic address: vandesandefh@gmail.com.
authors.forename: Françoise H
authors.initials: FH
authors.lastname: van de Sande
authors.affiliation: Department of Cariology, Endodontology and Pedodontology, Radboud University Medical Center, Philips van Leydenlaan 25, 6525 EX Nijmegen, The Netherlands.
authors.forename: Niek J M
authors.initials: NJ
authors.lastname: Opdam
authors.affiliation: Department of Cariology, Endodontology and Pedodontology, Radboud University Medical Center, Philips van Leydenlaan 25, 6525 EX Nijmegen, The Netherlands.
authors.forename: Gert Jan
authors.initials: GJ
authors.lastname: Truin
authors.affiliation: Department of Cariology, Endodontology and Pedodontology, Radboud University Medical Center, Philips van Leydenlaan 25, 6525 EX Nijmegen, The Netherlands.
authors.forename: Ewald M
authors.initials: EM
authors.lastname: Bronkhorst
authors.affiliation: Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), Gustav Mahler Laan 3004, 1081 LA Amsterdam, The Netherlands.
authors.forename: Johannes J
authors.initials: JJ
authors.lastname: de Soet
authors.affiliation: Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Gonçalves Chaves 457, 96015-560 Pelotas, RS, Brazil.
authors.forename: Maximiliano S
authors.initials: MS
authors.lastname: Cenci
authors.affiliation: Department of Cariology, Endodontology and Pedodontology, Radboud University Medical Center, Philips van Leydenlaan 25, 6525 EX Nijmegen, The Netherlands.
authors.forename: Marie-Charlotte
authors.initials: MC
authors.lastname: Huysmans
mesh_terms: D000368:Aged; D003188:Composite Resins / Q000737:chemistry; D003723:Dental Amalgam / Q000737:chemistry; D003731:Dental Caries / Q000209:etiology*; D003764:Dental Materials / Q000737:chemistry*; D003793:Dental Restoration, Permanent; D003804:Dentin / Q000473:pathology; D017220:Dentin-Bonding Agents / Q000737:chemistry; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008852:Microradiography / Q000379:methods; D008875:Middle Aged; D053761:Nanocomposites / Q000737:chemistry; D012008:Recurrence; D019279:Resin Cements / Q000737:chemistry; D062047:Silorane Resins / Q000737:chemistry; D016037:Single-Blind Method
chemical_list: C112828:Clearfil AP-X; C503075:Clearfil Protect Bond; C438313:Clearfil SE Bond; D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; C474035:Filtek Supreme; D008689:Methacrylates; D019279:Resin Cements; D062047:Silorane Resins; D003723:Dental Amalgam
keywords: Dental caries; Dental materials; Dentine-bonding agents; Microradiography; Tooth demineralization