title: Biophysiochemical properties of endothelial cells cultured on bio-inspired collagen films.
abstract: BACKGROUND
  In this study, we investigated the effect of the extracellular matrix on endothelial dysfunction by careful observation of human umbilical vein endothelial cells (HUVECs) cultured on denatured collagen film.
  
  
  RESULTS
  HUVECs on denatured collagen film showed relatively high surface roughness compared with normal HUVECs. The expression levels of MMP-1, MMP-2 and CD146 increased in the ECs on denatured collagen film. In addition, we examined the accumulation of fluorescent beads on HUVEC layers subjected to circulatory flow. The number of accumulated fluorescent beads increased on the disorganized HUVEC layers.
  
  
  CONCLUSIONS
  The proposed in vitro study using bio-inspired collagen films could potentially be used in the size- and ligand-based design of drugs to treat endothelial dysfunction caused by circulatory vascular diseases.
authors.forename: Eunseok
authors.initials: E
authors.lastname: Seo
authors.forename: Kyung Won
authors.initials: KW
authors.lastname: Seo
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Gil
authors.forename: Young-Ran
authors.initials: YR
authors.lastname: Ha
authors.forename: Eunseop
authors.initials: E
authors.lastname: Yeom
authors.forename: Seungchul
authors.initials: S
authors.lastname: Lee
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk 790-784, Korea. sjlee@postech.ac.kr.
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D051929:CD146 Antigen / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D018613:Microscopy, Confocal
chemical_list: D051929:CD146 Antigen; D003094:Collagen; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1