title: Differential expression analysis of a subset of GmNAC genes in shoots of two contrasting drought-responsive soybean cultivars DT51 and MTD720 under normal and drought conditions.
abstract: NAC transcription factors are known to be involved in regulation of plant responses to drought stress. In this study, the expression of 23 drought-responsive GmNAC genes was assessed in the shoot tissues of DT51 and MTD720, the two soybean varieties with contrasting drought-responsive phenotypes, by real-time quantitative PCR (RT-qPCR) under normal and drought conditions. Results indicated that expression profile of GmNAC genes was genotype-dependent, and six GmNACs (GmNAC019, 043, 062, 085, 095 and 101) had higher transcript levels in the shoots of the drought-tolerant DT51 in comparison with the drought-sensitive MTD720 under drought. Our study suggests a positive correlation between the higher drought tolerance degree of DT51 versus MTD720 and the up-regulation of at least these six drought-responsive GmNACs in the shoot tissues. Furthermore, on the basis of our analysis, three genes, GmNAC043, 085 and 101, were identified as promising candidates for development of drought-tolerant soybean cultivars by genetic engineering.
authors.affiliation: School of Biotechnology, International University, Vietnam National University HCMC, Block 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.
authors.forename: Nguyen Binh Anh
authors.initials: NB
authors.lastname: Thu
authors.forename: Xuan Lan Thi
authors.initials: XL
authors.lastname: Hoang
authors.forename: Hieu
authors.initials: H
authors.lastname: Doan
authors.forename: Thanh-Hao
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Dao
authors.initials: D
authors.lastname: Bui
authors.forename: Nguyen Phuong
authors.initials: NP
authors.lastname: Thao
authors.forename: Lam-Son Phan
authors.initials: LS
authors.lastname: Tran
mesh_terms: D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D005810:Multigene Family; D010641:Phenotype; D018749:RNA, Plant / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013025:Glycine max / Q000145:classification / Q000235:genetics*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D018749:RNA, Plant; D014157:Transcription Factors