title: Engineering terpene biosynthesis in Streptomyces for production of the advanced biofuel precursor bisabolene.
abstract: The past decade has witnessed a large influx of research toward the creation of sustainable, biologically derived fuels. While significant effort has been exerted to improve production capacity in common hosts, such as Escherichia coli or Saccharomyces cerevisiae, studies concerning alternate microbes comparatively lag. In an effort to expand the breadth of characterized hosts for fuel production, we map the terpene biosynthetic pathway in a model actinobacterium, Streptomyces venezuelae, and further alter secondary metabolism to afford the advanced biofuel precursor bisabolene. Leveraging information gained from study of the native isoprenoid pathway, we were able to increase bisabolene titer nearly 5-fold over the base production strain, more than 2 orders of magnitude greater than the combined terpene yield in the wild-type host. We also explored production on carbon sources of varying complexity to, notably, define this host as one able to perform consolidated bioprocessing.
authors.affiliation: †Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, California 94608, United States.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Phelan
authors.affiliation: ‡Department of Biotechnology, Norwegian University of Science and Technology, Sem Saelands vei 6/8, 7491 Trondheim, Norway.
authors.forename: Olga N
authors.initials: ON
authors.lastname: Sekurova
authors.affiliation: †Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, California 94608, United States.|∥Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 United States.
authors.forename: Jay D
authors.initials: JD
authors.lastname: Keasling
authors.affiliation: ‡Department of Biotechnology, Norwegian University of Science and Technology, Sem Saelands vei 6/8, 7491 Trondheim, Norway.
authors.forename: Sergey B
authors.initials: SB
authors.lastname: Zotchev
mesh_terms: D056804:Biofuels; D060847:Metabolic Engineering; D012717:Sesquiterpenes / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism
chemical_list: D056804:Biofuels; D012717:Sesquiterpenes
keywords: Streptomyces; biofuel; consolidated bioprocessing; isoprenoid; terpene