title: Cytomegalovirus alpha-chemokine genotypes are associated with clinical manifestations in children with congenital or postnatal infections.
abstract: Human cytomegalovirus (HCMV) is the leading cause of congenital infections. The aim of our study was to determine the prevalence of genotypes based on the highly polymorphic UL146 and UL147 HCMV genes and the relationship between the genotype and symptoms or viral load. We analyzed samples from 121 infants with symptomatic HCMV infection, including 32 congenitally infected newborns. The G7 and G5 genotypes were predominant in postnatal infection, whereas the G1 genotype was prevalent in congenital infection. Central nervous system (CNS) damage and hepatomegaly were detected more frequently among children infected with the G1 genotype than in those infected by other genotypes. An association between the viral genotype and viruria level was found. There was a strong correlation between HCMV genotypes determined through the UL146 and UL147 sequences (ĸ=0.794). In conclusion, we found that certain vCXCL genotypes are associated with clinical sequelae following HCMV infection.
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland. Electronic address: eparadowska@cbm.pan.pl.
authors.forename: Edyta
authors.initials: E
authors.lastname: Paradowska
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Jabłońska
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Płóciennikowska
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Mirosława
authors.initials: M
authors.lastname: Studzińska
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Suski
authors.affiliation: 3rd Department of Paediatrics, Polish Mother׳s Memorial Hospital, Research Institute, Lodz, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wiśniewska-Ligier
authors.affiliation: Department of Clinical Microbiology and Immunology, The Children׳s Memorial Health Institute, Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Dzierżanowska-Fangrat
authors.affiliation: Department of Clinical Microbiology and Immunology, The Children׳s Memorial Health Institute, Warsaw, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Kasztelewicz
authors.affiliation: 3rd Department of Paediatrics, Polish Mother׳s Memorial Hospital, Research Institute, Lodz, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Woźniakowska-Gęsicka
authors.affiliation: Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Zbigniew J
authors.initials: ZJ
authors.lastname: Leśnikowski
mesh_terms: D000328:Adult; D019743:Chemokines, CXC / Q000235:genetics*; D003587:Cytomegalovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000473:pathology* / Q000821:virology*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D014759:Viral Envelope Proteins / Q000235:genetics*; D019562:Viral Load; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D019743:Chemokines, CXC; D006023:Glycoproteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C101488:cytomegalovirus envelope protein gp116; C121017:viral chemokine CXC-1, Cytomegalovirus
keywords: Congenital infection; Human cytomegalovirus; Postnatal infection; Virus genotype; α-chemokine