title: Distinctive protein expression patterns of the strain Brevundimonas sp. ZF12 isolated from the aqueous zone containing high levels of radiation to cadmium-induced stress.
abstract: In the current study, different protein expression profiles in a strain Brevundimonas sp. ZF12, isolated from the aqueous zone containing high levels of radiation, were characterized following exposure to cadmium (II) using a proteomic strategy. In order to gain a deeper understanding of the cellular events that allow this strain to survive and undergo cadmium adaptation and sorption, the strain was tested under three experimental conditions of 5, 10 and 30 ppm cadmium (II) ions stress. Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry were used to identify the differentially expressed proteins under cadmium (II) stress. 20 differentially expressed spots were successfully identified by MS/MS analysis. These proteins are involved in DNA repair and protection, amino acid metabolism, nucleotide metabolism, energy homeostasis, oxidative stress response, redox homeostasis, protein folding and heat-shock response. The results obviously indicate that the ZF12 strain tends to endure the cadmium (II) stress conditions by modification in many aspects of its cellular physiology and metabolism.
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Masoudzadeh
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Alidoust
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
authors.forename: Nima
authors.initials: N
authors.lastname: Samie
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Hajfarajollah
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
authors.forename: Hakimeh
authors.initials: H
authors.lastname: Sharafi
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
authors.forename: Sima
authors.initials: S
authors.lastname: Modiri
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran.
authors.forename: Hossein Shahbani
authors.initials: HS
authors.lastname: Zahiri
authors.affiliation: Facility for Electron Microscopy Research, McGill University, 3640 Street, Montreal, Canada.
authors.forename: Hojatollah
authors.initials: H
authors.lastname: Vali
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran. Electronic address: Akbari@nigeb.ac.ir.
authors.forename: Kambiz Akbari
authors.initials: KA
authors.lastname: Noghabi
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D042063:Caulobacteraceae / Q000187:drug effects* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D045482:Hot Springs / Q000382:microbiology; D013058:Mass Spectrometry; D018384:Oxidative Stress; D020543:Proteome / Q000032:analysis / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D002104:Cadmium
keywords: Brevundimonas sp. ZF12; Cadmium (II); High background radiation area; Mass spectrometry; Proteomics