title: Enterobacteriaceae isolates carrying the New Delhi metallo-β-lactamase gene in Yemen.
abstract: Ten carbapenem-resistant Enterobacteriaceae (eight Klebsiella pneumoniae isolates and two Enterobacter cloacae) isolates from Yemen were investigated using in vitro antimicrobial susceptibility testing, phenotypic carbapenemase detection, multilocus sequence typing (MLST) and replicon typing. Carbapenemase, extended-spectrum β-lactamase (ESBL) and plasmid-mediated quinolone resistance determinant genes were identified using PCR and sequencing. All of the 10 carbapenem-resistant Enterobacteriaceae were resistant to β-lactams, tobramycin, ciprofloxacin and cotrimoxazole. Imipenem, doripenem and meropenem MICs ranged from 2 to >32 mg l(-1) and ertapenem MICs ranged from 6 to >32 mg l(-1). All of the K. pneumoniae isolates showed ESBL activity in phenotypic tests. Genes encoding blaNDM were detected in all strains. All K. pneumoniae strains produced CTX-M-15 ESBL and SHV β-lactamases. TEM-1 β-lactamase was detected in seven isolates. Nine isolates were qnr positive including QnrB1, QnrA1 and QnrS1, and six isolates produced AAC-6'-Ib-cr. MLST identified five different sequence types (STs): ST1399, ST147, ST29, ST405 and ST340. Replicon typing showed the presence of IncFII1K plasmids in four transformants. To the best of our knowledge, this is the first report of NDM-1-producing Enterobacteriaceae isolates in Yemen.
authors.affiliation: Laboratoire de Microbiologie Appliquée, FSNV, Université de Bejaia 06000, Algeria.
authors.forename: Alima
authors.initials: A
authors.lastname: Gharout-Sait
authors.affiliation: Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia 06000, Algeria.
authors.forename: Samer-Ahmed
authors.initials: SA
authors.lastname: Alsharapy
authors.affiliation: Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière, CHU Reims, Hôpital Robert Debré, Avenue du Général Koenig, 51092 Reims Cedex, France.
authors.forename: Lucien
authors.initials: L
authors.lastname: Brasme
authors.affiliation: Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia 06000, Algeria.
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Touati
authors.affiliation: Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia 06000, Algeria.
authors.forename: Rachida
authors.initials: R
authors.lastname: Kermas
authors.affiliation: Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia 06000, Algeria.
authors.forename: Sofiane
authors.initials: S
authors.lastname: Bakour
authors.affiliation: EA4687 UFR Médecine SFR CAP-Santé (FED 4231), Université de Reims-Champagne-Ardenne, Reims, France.|Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière, CHU Reims, Hôpital Robert Debré, Avenue du Général Koenig, 51092 Reims Cedex, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Guillard
authors.affiliation: EA4687 UFR Médecine SFR CAP-Santé (FED 4231), Université de Reims-Champagne-Ardenne, Reims, France.|Laboratoire de Bactériologie-Virologie-Hygiène Hospitalière, CHU Reims, Hôpital Robert Debré, Avenue du Général Koenig, 51092 Reims Cedex, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: de Champs
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016972:Enterobacter cloacae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D008297:Male; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D010957:Plasmids / Q000032:analysis; D016133:Polymerase Chain Reaction; D015006:Yemen / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1