title: Differentiation of reactive-like astrocytes cultured on nanofibrillar and comparative culture surfaces.
abstract: OBJECTIVE
  To investigate the directive importance of nanophysical properties on the morphological and protein expression responses of dibutyryladenosine cyclic monophosphate (dBcAMP)-treated cerebral cortical astrocytes in vitro.
  
  
  METHODS
  Elasticity and work of adhesion characterizations of culture surfaces were performed using atomic force microscopy and combined with previous surface roughness and polarity results. The morphological and biochemical differentiation of dBcAMP-treated astrocytes cultured on promising nanofibrillar scaffolds and comparative culture surfaces were investigated by immunocytochemistry, colocalization, super resolution microscopy and atomic force microscopy. The dBcAMP-treated astrocyte responses were further compared with untreated astrocyte responses.
  
  
  CONCLUSIONS
  Nanofibrillar scaffold properties were shown to reduce immunoreactivity responses while poly-L-lysine-functionalized Aclar® (Ted Pella Inc., CA, USA) properties were shown to induce responses reminiscent of glial scar formation. The comparison study indicated that directive cues may differ in wound-healing versus quiescent situations.
authors.affiliation: Electronic & Biological Nanostructures Laboratory, Department of Electrical and Computer Engineering, 428 S Shaw Lane, Michigan State University, Engineering Building Room 2120, East Lansing, MI, 48824, USA. tiryakiv@msu.edu.
authors.forename: Volkan Müjdat
authors.initials: VM
authors.lastname: Tiryaki
authors.forename: Virginia M
authors.initials: VM
authors.lastname: Ayres
authors.forename: Ijaz
authors.initials: I
authors.lastname: Ahmed
authors.forename: David I
authors.initials: DI
authors.lastname: Shreiber
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D003994:Bucladesine / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000378:metabolism; D004548:Elasticity; D018625:Microscopy, Atomic Force; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D003994:Bucladesine
keywords: astrocyte morphology; atomic force microscopy; confocal microscopy; elasticity; immunoreactivity; nanofibrillar scaffolds; surface energy