title: Neuroprotective Effect of Steamed and Fermented Codonopsis lanceolata.
abstract: Codonopsis lanceolata has been used as an herbal medicine for several lung inflammatory diseases, such as asthma, tonsillitis, and pharyngitis. Previously, we showed the neuroprotective effect of steamed and fermented C. lanceolata (SFC) in vitro and in vivo. In the current study, the treatment of HT22 cells with SFC decreased glutamate-induced cell death, suggesting that SFC protected HT22 cells from glutamate-induced cytotoxicity. Based on these, we sought to elucidate the mechanisms of the neuro-protective effect of SFC by measuring the oxidative stress parameters and the expression of Bax and caspase-3 in HT22 cells. SFC reduced contents of ROS, Ca(2+) and NO. Moreover, SFC restored contents of glutathione and glutathione reductase as well as inhibited Bax and caspase-3 activity in HT22 cells. These results indicate that steamed and fermented C. lanceolata (SFC) extract protected HT22 cells by anti-oxidative effect and inhibition of the expression of Bax and caspase-3.
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Yun
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Jiwoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Min Rye
authors.initials: MR
authors.lastname: Eom
authors.affiliation: Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Ko
authors.affiliation: Department of Teaics, Seowon University, Cheongju 361-742.
authors.forename: Hyeon Yong
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Department of Agrofood Resources, Functional food & Nutrition Division, Suwon 441-853.
authors.forename: Dong-Sik
authors.initials: DS
authors.lastname: Park
authors.affiliation: Newtree CO., LTD. 11F Tech Center, SKnTechno Park, 190-1, Sungnam 462-120, Republic of Korea.
authors.forename: Hee-Chul
authors.initials: HC
authors.lastname: Chung
authors.affiliation: Newtree CO., LTD. 11F Tech Center, SKnTechno Park, 190-1, Sungnam 462-120, Republic of Korea.
authors.forename: Jae Youn
authors.initials: JY
authors.lastname: Chung
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, College of Pharmacy, Kangwon National University, Chuncheon 200-701 ; Research Institute of Biotechnology, College of Pharmacy, Kangwon National University, Chuncheon 200-701.
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
keywords: Antioxidative activity; Bax; Caspase-3; Codonopsis lanceolata; Neuroprotective activity; Steam and fermentation