title: Isolation and identification of anti-tumor polysaccharide LSP21 from Limonium sinense (Girard) Kuntze.
abstract: Limonium sinense (Girard) Kuntze is a traditional Chinese folk medicine used for the treatment of fever, hemorrhage, hepatitis and other disorders. Recently, it was found that the crude polysaccharides from L. sinense (LSP) has significant anti-tumor activity. However, research on the isolation and identification of anti-tumor polysaccharide fractions from LSP has not yet been reported. In this study, three polysaccharides LSP11, LSP21, LSP31 were isolated and purified from LSP by using DEAE-52 cellulose column and Sephadex G-100 column chromatography. It was found that LSP21 exhibited the most significant inhibitory effect on the growth of HepG2 cells in vitro. Further research showed that LSP21 inhibited the growth of HepG2 cells in a dose-dependent manner and could induce cell body shrinkage, chromatin condensation, and reduction in the number of tumor cells with normal morphology which suggested that its cytotoxicity on tumor cell might be related to both inhibition on cell proliferation and inducement of cell death. Finally, the structural characteristics of LSP21 were analyzed by high performance liquid chromatography (HPLC) and gas chromatography (GC). The results showed that LSP21 is a heteropolysaccharide with an average molecular weight of 1.31×10(6) Da and consists of glucose, galactose and mannose in the ratio of 1.77:1:2.38.
authors.affiliation: Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Xiwang Road, Yancheng 224051, PR China.
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Tang
authors.affiliation: Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Xiwang Road, Yancheng 224051, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yu
authors.affiliation: Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Xiwang Road, Yancheng 224051, PR China; School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China. Electronic address: jinggao@ujs.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Xiwang Road, Yancheng 224051, PR China; School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Yan
authors.affiliation: Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Xiwang Road, Yancheng 224051, PR China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Dong
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D029606:Plumbaginaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D003911:Dextrans; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Anti-tumor; Isolation and purification; Limonium sinense (Girard) Kuntze; Polysaccharides; Structural characteristics