title: Fluoro-glycosyl acridinones are ultra-sensitive active site titrating agents for retaining β-glycosidases.
abstract: Novel fluorogenic 2-deoxy-2-fluoroglycosyl acridinone active site titrating reagents were synthesised and kinetic parameters determined for their inactivation of two retaining β-glucosidases, a β-galactosidase, a β-xylosidase and several cellulases. Fluorescence-monitored active site titration using this class of reagents reliably measured active enzyme concentrations down to 3 nM.
authors.affiliation: The Department of Chemistry, The University of British Columbia, 2036 Main Mall, Vancouver, BC, Canada V6T 1Z1. withers@chem.ubc.ca.
authors.forename: Tianmeng
authors.initials: T
authors.lastname: Duo
authors.forename: Ethan D
authors.initials: ED
authors.lastname: Goddard-Borger
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Withers
mesh_terms: D000166:Acridines / Q000737:chemistry*; D020134:Catalytic Domain; D007700:Kinetics; D001616:beta-Galactosidase / Q000037:antagonists & inhibitors / Q000737:chemistry*
chemical_list: D000166:Acridines; D001616:beta-Galactosidase