title: Effect of a Ru(II) polypyridyl complex [Ru(bpy)2(mdpz)]2+ on the stabilization of the RNA triplex poly(U)·poly(A)*poly(U).
abstract: There is renewed interest in investigating triplex nucleic acids because triplexes may be implicated in a range of cellular functions. However, the stabilization of triplex nucleic acids is essential to achieve their biological functions. In contrast to triplex DNA, little has been reported concerning the recognition of triplex RNA by transition-metal complexes at present. We report here a ruthenium(ii) polypyridyl complex, [Ru(bpy)2(mdpz)](2+) (bpy = 2,2'-bipyridine; mdpz = 7,7'-methylenedioxyphenyl-dipyrido-[3,2-a:2',3'-c]phenazine), as a sensitive luminescent probe for poly(U)·poly(A)*poly(U), which can strongly stabilize the triplex RNA from 37.5 to 53.1 °C in solution. The main results further advance our knowledge on the triplex RNA-binding by metal complexes, particularly ruthenium(ii) complexes.
authors.affiliation: College of Chemistry, Xiangtan University, Xiangtan 411105, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: He
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Lifeng
authors.initials: L
authors.lastname: Tan
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000494:pharmacology*; D011061:Poly A / Q000737:chemistry / Q000378:metabolism; D011072:Poly U / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism*; D020871:RNA Stability / Q000187:drug effects*; D044366:Transition Temperature; D014783:Viscosity
chemical_list: D009942:Organometallic Compounds; C103599:bis(bipyridyl)ruthenium(II); D011061:Poly A; D011072:Poly U; D015082:2,2'-Dipyridyl; D012313:RNA