title: MicroRNA-27a promotes porcine myoblast proliferation by downregulating myostatin expression.
abstract: MicroRNAs are endogenous ~22nt RNAs that negatively regulate gene expression at the posttranscriptional level via binding to the 3'-untranslated region (3'UTR) of target mRNAs. The microRNA miR-27a was reported to depress the expression of myostatin, a critical inhibitor of skeletal myogenesis, by binding to its 3'UTR in mouse. In this study, we cloned the full-length 3'UTR of porcine myostatin by rapid amplification of 3'-cDNA ends (3'-RACE) and demonstrated that the 3'UTR of porcine myostatin is targeted by miR-27a. The phenomenon that the level of myostatin inversely correlated with miR-27a was observed in fat and heart of pigs and also in proliferating porcine myoblasts. Besides, overexpression of miR-27a in porcine myoblasts promoted cell proliferation by reducing the expression of myostatin. Our data suggest that miR-27a positive regulates porcine myoblast proliferation via targeting myostatin.
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: T
authors.initials: T
authors.lastname: Yang
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: X L
authors.initials: XL
authors.lastname: Chen
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Huang
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: W X
authors.initials: WX
authors.lastname: Wen
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: M
authors.initials: M
authors.lastname: Xu
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: D W
authors.initials: DW
authors.lastname: Chen
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: B
authors.initials: B
authors.lastname: Yu
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: J
authors.initials: J
authors.lastname: He
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Luo
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: X B
authors.initials: XB
authors.lastname: Mao
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,P. R. China.
authors.forename: P
authors.initials: P
authors.lastname: Zheng
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics*; D005260:Female; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D032446:Myoblasts / Q000378:metabolism; D055435:Myostatin / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D055435:Myostatin