title: Reversible detection of vancomycin using peptide-functionalized cantilever array sensor.
abstract: A reversible detection method for vancomycin was developed utilizing the cantilever array sensor functionalized by a designed peptide consisting of a cysteine (Cys-), a space linker (-Gly-Gly-Gly-Gly-) and a molecular recognition ligand (-L-Lys-D-Ala-D-Ala). It was found that the peptide space linker was necessary and important for the response of the cantilever array sensor. The sensing cantilevers in the array were functionalized with the peptide while the reference cantilevers were modified by 6-mercapto-1-hexanol (MCH) to eliminate the influence of environmental disturbances. The binding between vancomycin and the peptide induced a change of surface stresses in the sensing cantilevers resulting in a differential deflection between the sensing and reference cantilevers. The reciprocal of the differential deflection is linear with the reciprocal of vancomycin concentration within the range of 2 μM to 100 μM (R=0.993) at a detection limit of 0.2 μM (S/N=3). The reversible detection can be realized just by regenerating the sensing cantilevers with running buffer solution. Other antibiotics such as doxycycline, streptomycin, and kanamycin have negligible effect on the response of the sensor. The sensor can also be utilized for reversible detection of vancomycin in serum background, which clearly indicates the potential of the sensor for vancomycin detection in real biological samples.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Baoping
authors.initials: B
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: blzhang@ciac.ac.cn.
authors.forename: Bailin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: jltang@ciac.ac.cn.
authors.forename: Jilin
authors.initials: J
authors.lastname: Tang
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D008024:Ligands; D057230:Limit of Detection; D009842:Oligopeptides / Q000737:chemistry; D014640:Vancomycin / Q000032:analysis* / Q000097:blood
chemical_list: D000900:Anti-Bacterial Agents; D008024:Ligands; D009842:Oligopeptides; D014640:Vancomycin
keywords: Cantilever array sensor; Peptide; Reversibility; Serum; Vancomycin