title: Human dendritic cell activation induced by a permannosylated dendron containing an antigenic GM3-lactone mimetic.
abstract: Vaccination strategies based on dendritic cells (DCs) armed with specific tumor antigens have been widely exploited due the properties of these immune cells in coordinating an innate and adaptive response. Here, we describe the convergent synthesis of the bifunctional multivalent glycodendron 5, which contains nine residues of mannose for DC targeting and one residue of an immunogenic mimetic of a carbohydrate melanoma associated antigen. The immunological assays demonstrated that the glycodendron 5 is able to induce human immature DC activation in terms of a phenotype expression of co-stimulatory molecules expression and MHCII. Furthermore, DCs activated by the glycodendron 5 stimulate T lymphocytes to proliferate in a mixed lymphocytes reaction (MLR).
authors.affiliation: Glycosystems Laboratory, Instituto de Investigaciones Químicas (IIQ), CSIC - Universidad de Sevilla, Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Renato
authors.initials: R
authors.lastname: Ribeiro-Viana
authors.affiliation: Department of NEUROFARBA, University of Florence, Viale Pieraccini 6, 50134 Firenze, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Bonechi
authors.affiliation: Glycosystems Laboratory, Instituto de Investigaciones Químicas (IIQ), CSIC - Universidad de Sevilla, Américo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Rojo
authors.affiliation: Department of NEUROFARBA, University of Florence, Viale Pieraccini 6, 50134 Firenze, Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Ballerini
authors.affiliation: Department of Biochemical Science, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Comito
authors.affiliation: Department of Chemistry, University of Florence, Via della Lastruccia 13, 50019 Sesto Fiorentino (FI), Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Richichi
authors.affiliation: Department of Chemistry, University of Florence, Via della Lastruccia 13, 50019 Sesto Fiorentino (FI), Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Nativi
keywords: DC targeting; DC-SIGN; GM3-lactone mimetic; cancer immunotherapy; glycodendron; multivalent glycosystems; multivalent interactions