title: Alternations in neuroendocrine and endocrine regulation of reproduction in male goldfish (Carassius auratus) following an acute and chronic exposure to vinclozolin, in vivo.
abstract: The fungicide vinclozolin (VZ) is in use globally and known to disrupt reproductive function in male. The present study tested the hypothesis that VZ disrupts testicular function in goldfish (Carassius auratus) by affecting brain-pituitary-testis axis. Goldfish were exposed to 100, 400 and 800 μg/L VZ and 5 μg/L 17β-estradiol (E2) for comparison. In VZ treated goldfish, 11-ketotesteosterone (11-KT) secretion was changed depending on dose and duration period of treatment. Following 7 days of exposure, 11-KT was decreased in goldfish exposed to 800 μg/L VZ, while it was increased in goldfish exposed to 100 μg/L VZ after 30 days of exposure. Circulating E2 level was unchanged in VZ treated goldfish, however the E2/11-KT ratio was increased in a concentration-related manner. In E2 treated goldfish, circulatory 11-KT and E2 levels were decreased and increased, respectively, which resulted in an increase in the E2/11-KT ratio. Exposure to VZ at 100 μg/L caused a significant increase in the circulatory luteinizing hormone (LH) after 30 days. In E2 treated fish circulatory LH was decreased, significantly. Transcripts of genes encoding gonadotropin-releasing hormone and androgen receptor in the brain, and those of genes encoding LH and follicle-stimulating hormone receptors, StAR, CYP17, and 3β-HSD in the testis changed in VZ-treated goldfish depending on concentration and period of treatment. mRNA of genes encoding vitellogenin and estrogen receptor in the liver and cytochrome P450 aromatase in the brain were increased in E2-treated goldfish. The results suggest that VZ-induced changes in 11-KT were due to disruption in brain-pituitary-testis axis and provide integrated characterization of VZ-related reproductive disorders in male fish.
authors.affiliation: Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25 Vodňany, Czech Republic.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Golshan
authors.affiliation: Veterinary Biomedical Sciences Department, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada; Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25 Vodňany, Czech Republic.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Hatef
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
authors.forename: Ava
authors.initials: A
authors.lastname: Zare
authors.affiliation: Department of Ichthyobiology and Fisheries, University of Agriculture, Kraków 30-199, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Socha
authors.affiliation: Research Unit Animal and Functionalities of Animal Products, INRA, University of Lorraine, Vandoeuvre-lès-Nancy 54505, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Milla
authors.affiliation: Department of Ichthyobiology and Fisheries, University of Agriculture, Kraków 30-199, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Gosiewski
authors.affiliation: Research Unit Animal and Functionalities of Animal Products, INRA, University of Lorraine, Vandoeuvre-lès-Nancy 54505, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Fontaine
authors.affiliation: Department of Ichthyobiology and Fisheries, University of Agriculture, Kraków 30-199, Poland.
authors.forename: Mirosława
authors.initials: M
authors.lastname: Sokołowska-Mikołajczyk
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Habibi
authors.affiliation: Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 165 21 Prague 6, Czech Republic; Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25 Vodňany, Czech Republic. Electronic address: alavi@af.czu.cz.
authors.forename: Sayyed Mohammad Hadi
authors.initials: SM
authors.lastname: Alavi
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D006054:Goldfish; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010080:Oxazoles / Q000008:administration & dosage / Q000633:toxicity*; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects / Q000502:physiology; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D014819:Vitellogenins / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D010080:Oxazoles; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D001141:Aromatase; C025643:vinclozolin
keywords: Cytochrome P450 aromatase; Gonadotropin-releasing hormone; Luteinizing hormone; Sex steroids; Steroidogenic enzyme genes