title: Mass spectrometry and multiplex antigen assays to assess microbial quality and toxin production of Staphylococcus aureus strains isolated from clinical and food samples.
abstract: The aim of our study was to investigate the microbial quality of meat products and on some clinical samples in Abidjan focused on Staphylococcus genus and the toxin production profile of Staphylococcus aureus (S. aureus) isolated. Bacteria were collected from 240 samples of three meat products sold in Abidjan and 180 samples issued from clinical infections. The strains were identified by both microbiological and MALDI-TOF-MS methods. The susceptibility to antibiotics was determined by the disc diffusion method. The production of Panton-Valentine Leukocidin, LukE/D, and epidermolysins was screened using radial gel immunodiffusion. The production of staphylococcal enterotoxins and TSST-1 was screened by a Bio-Plex Assay. We observed that 96/240 of meat samples and 32/180 of clinical samples were contaminated by Staphylococcus. Eleven species were isolated from meats and 4 from clinical samples. Forty-two S. aureus strains were isolated from ours samples. Variability of resistance was observed for most of the tested antibiotics but none of the strains displays a resistance to imipenem and quinolones. We observed that 89% of clinical S. aureus were resistant to methicillin against 58% for those issued from meat products. All S. aureus isolates issued from meat products produce epidermolysins whereas none of the clinical strains produced these toxins. The enterotoxins were variably produced by both clinical and meat product samples.
authors.affiliation: Laboratoire de Biotechnologie et Microbiologie des Aliments, Faculté des Sciences et Technologies des Aliments, Université Nangui Abroguoua, BP 801 Abidjan 02, Cote D'Ivoire ; Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin ; Laboratoire de Bactériologie et Virologie, Faculté des Sciences Médicales, Centre Hospitalier et Universitaire de Treichville, BP V3 Abidjan, Cote D'Ivoire.
authors.forename: Paul
authors.initials: P
authors.lastname: Attien
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Haziz
authors.initials: H
authors.lastname: Sina
authors.affiliation: Université de Strasbourg (CHRU Strasbourg), Fédération de Médecine Translationnelle de Strasbourg, EA 7290 Virulence Bactérienne Précoce, Institut de Bactériologie, 3 rue Koeberlé, 67000 Strasbourg, France.
authors.forename: Wardi
authors.initials: W
authors.lastname: Moussaoui
authors.affiliation: Université de Strasbourg (CHRU Strasbourg), Fédération de Médecine Translationnelle de Strasbourg, EA 7290 Virulence Bactérienne Précoce, Institut de Bactériologie, 3 rue Koeberlé, 67000 Strasbourg, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Zimmermann-Meisse
authors.affiliation: Laboratoire de Biotechnologie et Microbiologie des Aliments, Faculté des Sciences et Technologies des Aliments, Université Nangui Abroguoua, BP 801 Abidjan 02, Cote D'Ivoire.
authors.forename: Thomas
authors.initials: T
authors.lastname: Dadié
authors.affiliation: Université de Strasbourg (CHRU Strasbourg), Fédération de Médecine Translationnelle de Strasbourg, EA 7290 Virulence Bactérienne Précoce, Institut de Bactériologie, 3 rue Koeberlé, 67000 Strasbourg, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Keller
authors.affiliation: Université de Strasbourg (CHRU Strasbourg), Fédération de Médecine Translationnelle de Strasbourg, EA 7290 Virulence Bactérienne Précoce, Institut de Bactériologie, 3 rue Koeberlé, 67000 Strasbourg, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Riegel
authors.affiliation: Laboratoire de Bactériologie et Virologie, Faculté des Sciences Médicales, Centre Hospitalier et Universitaire de Treichville, BP V3 Abidjan, Cote D'Ivoire.
authors.forename: Vincent
authors.initials: V
authors.lastname: Edoh
authors.affiliation: Department of Biology and Center for Computational and Integrative Biology, Rutgers University, 315 Penn Street, Camden, NJ 08102, USA.
authors.forename: Simeon O
authors.initials: SO
authors.lastname: Kotchoni
authors.affiliation: Laboratoire de Biotechnologie et Microbiologie des Aliments, Faculté des Sciences et Technologies des Aliments, Université Nangui Abroguoua, BP 801 Abidjan 02, Cote D'Ivoire.
authors.forename: Marcellin
authors.initials: M
authors.lastname: Djè
authors.affiliation: Université de Strasbourg (CHRU Strasbourg), Fédération de Médecine Translationnelle de Strasbourg, EA 7290 Virulence Bactérienne Précoce, Institut de Bactériologie, 3 rue Koeberlé, 67000 Strasbourg, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Prévost
authors.affiliation: Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
authors.forename: Lamine
authors.identifier: 0000-0002-8160-5954
authors.initials: L
authors.lastname: Baba-Moussa
mesh_terms: D000942:Antigens, Bacterial / Q000096:biosynthesis*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D005516:Food Microbiology; D006801:Humans; D013058:Mass Spectrometry; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins