title: Evaluation of diuretic activity of alcoholic extract of roots of cissampelos pareira in albino rats.
abstract: BACKGROUND
  In congestive heart failure, nephritis, toxemia of pregnancy, premenstrual tension and hypertension associated with oedema diuretic compounds are much helpful to relieve these conditions.
  
  
  OBJECTIVE
  To study the diuretic activity of alcoholic extract of roots of Cissampelos pareira by Lipschitz method in albino rats.
  
  
  METHODS
  Five groups of Albino rats were used to evaluate the diuretic activity of alcoholic extract of roots of Cissampelos pareira by using metabolic cages. The group I serves as normal control received vehicle (2% CMC in normal saline), group II with Furosemide (10 mg/Kg, p.o), Groups III, IV and V with low (100 mg/kg), medium (200 mg/kg), and high (400 mg/kg) doses of alcoholic extract of roots of Cissampelos pareira respectively. Immediately after the alcoholic extract of roots of Cissampelos pareira treatment all the rats were hydrated with saline (15 ml/kg, p.o) and 2 animals placed in each metabolic cage, kept at 21°C±0.5°C. No food and water was made available to animals for 5 hour. The total volume of urine collected with each metabolic cage was measured at the end of 5 hour. Various parameters like total urine volume and concentration of different ions i.e.; Sodium, Potassium , Chloride in the urine were measured.
  
  
  RESULTS
  In this model when compared to control group the alcoholic extract of roots of Cissampelos pareira treated groups at different dose levels (100,200 and 400 mg/kg) have noted with significant increase in the urine volume and also significantly enhanced the excretion of Sodium, Potassium and Chloride ions in urine.
  
  
  CONCLUSIONS
  RESULTS showed that single dose administration of standard Furosemide and alcoholic extract of roots of Cissampelos pareira significantly (p<0.05*, p<0.01(**), p<0.001***) increased the urine output along with an increase in elimination of Sodium, Potassium, and Chloride ions. Alcoholic extract of roots of Cissampelos pareira 400 mg/Kg produced a comparable diuretic activity with standard Furosemide.
authors.affiliation: Ph.D. Scholar, Department of Pharmacology, KIMS University , Karad, Maharashtra, India .
authors.forename: Suresh Babu
authors.initials: SB
authors.lastname: Sayana
authors.affiliation: Professor & HOD, Department of Pharmacology, KIMS University , Karad, Maharashtra, India .
authors.forename: Chitra C
authors.initials: CC
authors.lastname: Khanwelkar
authors.affiliation: Professor & HOD, Department of Pharmacology, VL College of Pharmacy , Raichur, Karnataka, India .
authors.forename: Venkat Rao
authors.initials: VR
authors.lastname: Nimmagadda
authors.affiliation: Principal, Vikas College of Pharmacy , Visannapeta, Andhra Pradesh, India .
authors.forename: Jeevan Mani Babu
authors.initials: JM
authors.lastname: Dasi
authors.affiliation: Professor & HOD, Department of Pharmacology, RIMS , Raichur, Karnataka, India .
authors.forename: Vasant R
authors.initials: VR
authors.lastname: Chavan
authors.affiliation: Assistant Professor, Department of Pharmacology, Bojjam Narasimhulu College of Pharmacy , Vinay Nagar, Hyderabad, Andhra Pradesh, India .
authors.forename: Aruna
authors.initials: A
authors.lastname: Kutani
authors.affiliation: Drug Safety Associate, Quintiles Technologies India Private Limited , Bengaluru, Karnataka, India .
authors.forename: Karthik
authors.initials: K
authors.lastname: Kotagiri
keywords: Alcoholic extract; C.pareira; Diuretic activity; Hydrated rats; Roots