title: A RAD52 genetic variant located in a miRNA binding site is associated with glioma risk in Han Chinese.
abstract: As a crucial homologous recombination repair gene, RAD52 participates in maintenance of genomic stability and prevention of tumorigenesis. Although several cancer susceptibility RAD52 single nucleotide polymorphisms (SNPs) have been identified previously, little was known on how the RAD52 SNPs are involved in glioma development in Han Chinese. Therefore, we examined the association between five RAD52 SNPs (rs1051669, rs10774474, rs11571378, rs7963551 and rs6489769) and glioma risk using a case-control design. Odds ratios (ORs) and 95 % confidence intervals (CIs) were estimated by logistic regression. We found that only the RAD52 rs7963551 SNP was significantly associated with glioma risk, with the odds of having the rs7963551 AC or CC genotype in patients was 0.49 (95 % CI 0.37-0.65, P = 9.2 × 10(-6)) or 0.39 (95 % CI 0.18-0.81, P = 0.012) compared with the AA genotype. These data are consistent with functional relevance of allelic regulation of RAD52 expression by the rs7963551 SNP and miRNA let-7 in cancer cells. Stratified analyses elucidated that statistically significant association between glioma and rs7963551 SNP only existed in either astrocytic tumors (P = 6.3 × 10(-6)) or oligoastrocytic tumors (P = 0.002). In conclusion, our results support the hypothesis that genetic variants influencing miRNA-mediated regulation of tumor suppressor genes or oncogenes may contribute glioma susceptibility.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, P. O. Box 53, Beijing, 100029, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.forename: Yi-Dong
authors.initials: YD
authors.lastname: Chen
authors.forename: Sichong
authors.initials: S
authors.lastname: Han
authors.forename: Jinyu
authors.initials: J
authors.lastname: Wei
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Ge
authors.forename: Wenting
authors.initials: W
authors.lastname: Pan
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Qiu
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D001665:Binding Sites; D001932:Brain Neoplasms / Q000209:etiology* / Q000473:pathology; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005910:Glioma / Q000209:etiology* / Q000473:pathology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D051721:Rad52 DNA Repair and Recombination Protein / Q000235:genetics*; D012307:Risk Factors; D055815:Young Adult
chemical_list: D035683:MicroRNAs; C497194:RAD52 protein, human; D051721:Rad52 DNA Repair and Recombination Protein