title: Optimisation extraction of chondroitin sulfate from fish bone by high intensity pulsed electric fields.
abstract: High intensity pulsed electric fields (PEF) was used to extract chondroitin sulphate (CS) from fish bone. Results show that PEF extraction speed is much faster, and the content of CS is much higher compared with traditional methods. Variation of PEF parameters and the content of CS were determined by single factor experiments. The processing conditions were optimised by quadratic general rotary unitised design experiments. The maximum yield of 6.92 g/L was achieved under the following conditions: material-liquid ratio of 1:15 g/mL, electric field intensity of 16.88 kV/cm, pulse number of 9, and NaOH concentration of 3.24%. The purity of CS was analysed by agarose gel electrophoresis. CS purity was high, and the extract did not contain any other glycosaminoglycans. PEF can be widely used to extract CS with non-thermal performance, high speed, and low pollution.
authors.affiliation: College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China. Electronic address: hgd@jlu.edu.cn.
authors.forename: Guidan
authors.initials: G
authors.lastname: He
authors.affiliation: College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China.
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Yin
authors.affiliation: College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Yan
authors.affiliation: College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Yu
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification*; D004560:Electricity; D055664:Electrochemical Techniques / Q000379:methods*; D005399:Fishes
chemical_list: D002809:Chondroitin Sulfates
keywords: Chondroitin sulphate; Extraction; Fish bone; High intensity pulsed electric fields (PEF); Quadratic general rotary unitised design