title: Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography.
abstract: The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.
authors.affiliation: Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Erin E H
authors.initials: EE
authors.lastname: Tran
authors.affiliation: Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Simmons
authors.affiliation: Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Alberto
authors.initials: A
authors.lastname: Bartesaghi
authors.affiliation: Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Shoemaker
authors.affiliation: Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Nelson
authors.affiliation: Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
authors.forename: Judith M
authors.initials: JM
authors.lastname: White
authors.affiliation: Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA ss1@nih.gov.
authors.forename: Sriram
authors.initials: S
authors.lastname: Subramaniam
mesh_terms: D020285:Cryoelectron Microscopy; D029043:Ebolavirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D019142:Hemorrhagic Fever, Ebola / Q000821:virology*; D006801:Humans; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus