title: Histopathological and immunohistochemical analysis of melt-associated retroprosthetic membranes in the Boston type 1 keratoprosthesis.
abstract: OBJECTIVE
  Retroprosthetic membrane (RPM) formation is the most common complication associated with the Boston type 1 keratoprosthesis and has been associated with corneal melt.
  
  
  OBJECTIVE
  To identify the histological and immunohistochemical characteristics of RPMs associated with corneal melt.
  
  
  METHODS
  Observational histopathological case series at a tertiary eye care referral center among patients who underwent Boston type 1 keratoprosthesis explantation because of donor corneal melt at the Illinois Eye and Ear Infirmary between January 1, 2011, and January 1, 2012.
  
  
  METHODS
  Seven RPM specimens from 7 eyes were stained with hematoxylin-eosin, cytokeratin 7, cytokeratin AE1/3, smooth muscle actin, vimentin, and CD34. Light microscopy was used to evaluate specimens for inflammation and epithelial ingrowth. XY-karyotyping using fluorescence in situ hybridization was performed on 4 specimens with known donor-recipient sex mismatch.
  
  
  METHODS
  Histological and immunohistochemical characteristics of RPMs.
  
  
  RESULTS
  Inflammatory cells were present in 4 of 7 RPMs. In 3 of 4 sex-mismatched specimens, tissue XY-karyotyping of the RPM interphase cells was consistent with the host sex karyotype. The fourth specimen showed a mixture of recipient-type and donor-type cells.
  
  
  CONCLUSIONS
  Melt-associated RPMs show variable degrees of inflammation. Most membranes seem to originate from a proliferation of host cells, but donor tissue may contribute in some cases.
authors.affiliation: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago.
authors.forename: Joshua H
authors.initials: JH
authors.lastname: Hou
authors.affiliation: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago.
authors.forename: Kavitha R
authors.initials: KR
authors.lastname: Sivaraman
authors.affiliation: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago.
authors.forename: Jose
authors.initials: J
authors.lastname: de la Cruz
authors.affiliation: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago2Department of Pathology, University of Illinois at Chicago.
authors.forename: Amy Y
authors.initials: AY
authors.lastname: Lin
authors.affiliation: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago.
authors.forename: Maria Soledad
authors.initials: MS
authors.lastname: Cortina
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001187:Artificial Organs; D015415:Biomarkers / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003315:Cornea; D003316:Corneal Diseases / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D020878:Device Removal; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008566:Membranes / Q000378:metabolism / Q000473:pathology*; D019736:Prostheses and Implants; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; D005136:Eye Proteins