title: Intraindividual genome expression analysis reveals a specific molecular signature of psoriasis and eczema.
abstract: Previous attempts to gain insight into the pathogenesis of psoriasis and eczema by comparing their molecular signatures were hampered by the high interindividual variability of those complex diseases. In patients affected by both psoriasis and nonatopic or atopic eczema simultaneously (n = 24), an intraindividual comparison of the molecular signatures of psoriasis and eczema identified genes and signaling pathways regulated in common and exclusive for each disease across all patients. Psoriasis-specific genes were important regulators of glucose and lipid metabolism, epidermal differentiation, as well as immune mediators of T helper 17 (TH17) responses, interleukin-10 (IL-10) family cytokines, and IL-36. Genes in eczema related to epidermal barrier, reduced innate immunity, increased IL-6, and a TH2 signature. Within eczema subtypes, a mutually exclusive regulation of epidermal differentiation genes was observed. Furthermore, only contact eczema was driven by inflammasome activation, apoptosis, and cellular adhesion. On the basis of this comprehensive picture of the pathogenesis of psoriasis and eczema, a disease classifier consisting of NOS2 and CCL27 was created. In an independent cohort of eczema (n = 28) and psoriasis patients (n = 25), respectively, this classifier diagnosed all patients correctly and also identified initially misdiagnosed or clinically undifferentiated patients.
authors.affiliation: Center of Allergy & Environment, Technische Universität and Helmholtz Center Munich, Member of the German Center for Lung Research, 80802 Munich, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Quaranta
authors.affiliation: Institute of Computational Biology, Helmholtz Center Munich, 85764 Neuherberg, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Knapp
authors.affiliation: Department of Dermatology and Allergy, Technische Universität Munich, 80802 Munich, Germany.
authors.forename: Natalie
authors.initials: N
authors.lastname: Garzorz
authors.affiliation: Center of Allergy & Environment, Technische Universität and Helmholtz Center Munich, Member of the German Center for Lung Research, 80802 Munich, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Mattii
authors.affiliation: Division of Genetics and Molecular Medicine, King's College London School of Medicine, Guy's Hospital, London WC2R 2LS, UK.
authors.forename: Venu
authors.initials: V
authors.lastname: Pullabhatla
authors.affiliation: Center of Allergy & Environment, Technische Universität and Helmholtz Center Munich, Member of the German Center for Lung Research, 80802 Munich, Germany.
authors.forename: Davide
authors.initials: D
authors.lastname: Pennino
authors.affiliation: Department of Dermatology and Allergy, Technische Universität Munich, 80802 Munich, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Andres
authors.affiliation: Department of Dermatology and Allergy, Technische Universität Munich, 80802 Munich, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Traidl-Hoffmann
authors.affiliation: Laboratory of Experimental Immunology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico, 00163 Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cavani
authors.affiliation: Institute of Computational Biology, Helmholtz Center Munich, 85764 Neuherberg, Germany. Department of Mathematics, Technische Universität Munich, 85748 Garching, Germany.
authors.forename: Fabian J
authors.initials: FJ
authors.lastname: Theis
authors.affiliation: Department of Dermatology and Allergy, Technische Universität Munich, 80802 Munich, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Ring
authors.affiliation: Center of Allergy & Environment, Technische Universität and Helmholtz Center Munich, Member of the German Center for Lung Research, 80802 Munich, Germany.
authors.forename: Carsten B
authors.initials: CB
authors.lastname: Schmidt-Weber
authors.affiliation: Center of Allergy & Environment, Technische Universität and Helmholtz Center Munich, Member of the German Center for Lung Research, 80802 Munich, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Eyerich
authors.affiliation: Department of Dermatology and Allergy, Technische Universität Munich, 80802 Munich, Germany. kilian.eyerich@lrz.tum.de.
authors.forename: Kilian
authors.initials: K
authors.lastname: Eyerich
mesh_terms: D000328:Adult; D015331:Cohort Studies; D004485:Eczema / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D025341:Principal Component Analysis; D011565:Psoriasis / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D015398:Signal Transduction / Q000235:genetics