title: Endogenous synthesis of 2-aminoacrylate contributes to cysteine sensitivity in Salmonella enterica.
abstract: RidA, the archetype member of the widely conserved RidA/YER057c/UK114 family of proteins, prevents reactive enamine/imine intermediates from accumulating in Salmonella enterica by catalyzing their hydrolysis to stable keto acid products. In the absence of RidA, endogenous 2-aminoacrylate persists in the cellular environment long enough to damage a growing list of essential metabolic enzymes. Prior studies have focused on the dehydration of serine by the pyridoxal 5'-phosphate (PLP)-dependent serine/threonine dehydratases, IlvA and TdcB, as sources of endogenous 2-aminoacrylate. The current study describes an additional source of endogenous 2-aminoacrylate derived from cysteine. The results of in vivo analysis show that the cysteine sensitivity of a ridA strain is contingent upon CdsH, the predominant cysteine desulfhydrase in S. enterica. The impact of cysteine on 2-aminoacrylate accumulation is shown to be unaffected by the presence of serine/threonine dehydratases, revealing another mechanism of endogenous 2-aminoacrylate production. Experiments in vitro suggest that 2-aminoacrylate is released from CdsH following cysteine desulfhydration, resulting in an unbound aminoacrylate substrate for RidA. This work expands our understanding of the role played by RidA in preventing enamine stress resulting from multiple normal metabolic processes.
authors.affiliation: Department of Microbiology, University of Georgia, Athens, Georgia, USA.
authors.forename: Dustin C
authors.initials: DC
authors.lastname: Ernst
authors.affiliation: Department of Microbiology, University of Georgia, Athens, Georgia, USA.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Lambrecht
authors.affiliation: Department of Microbiology, University of Georgia, Athens, Georgia, USA.
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Schomer
authors.affiliation: Department of Microbiology, University of Georgia, Athens, Georgia, USA dmdowns@uga.edu.
authors.forename: Diana M
authors.initials: DM
authors.lastname: Downs
mesh_terms: D000179:Acrylates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003545:Cysteine / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D009154:Mutation; D019779:Salmonella enterica / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D000179:Acrylates; D001426:Bacterial Proteins; D003545:Cysteine