title: Catalpol ameliorates sodium taurocholate-induced acute pancreatitis in rats via inhibiting activation of nuclear factor kappa B.
abstract: Catalpol, an iridoid glucoside extracted from the traditional Chinese herbal medicine, Rehmannia glutinosa, is reported to exert neuroprotective, anti-inflammatory, anti-tumor and anti-apoptotic effects. The main aim of the present study was to investigate whether catalpol ameliorates experimental acute pancreatitis (AP) induced by sodium taurocholate (STC). AP was induced in rats via retrograde injection of 4% STC (0.1 mL/100 g) into the biliopancreatic duct. Rats were pre-treated with saline or catalpol (50 mg/kg) 2 h before STC injection. At 12, 24 and 48 h after injection, the severity of AP was evaluated using biochemical and morphological analyses. Pretreatment with catalpol led to a significant reduction in serum amylase and lipase activities, pancreatic histological damage, myeloperoxidase (MPO) activity, interleukin (IL)-1β, IL-6 and TNF-α levels, and activation of nuclear factor kappa B (NF-κB). Moreover, administration of catalpol increased the viability of pancreatic acinar cells and inhibited NF-κB expression in vitro. Our results collectively support the potential of catalpol as a highly effective therapeutic agent for treatment of AP.
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Zhabei District, Shanghai 200072, China. zhunikaixin_xwq@163.com.
authors.forename: Wen Qin
authors.initials: WQ
authors.lastname: Xiao
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Zhabei District, Shanghai 200072, China. ygj234@163.com.
authors.forename: Guo Jian
authors.initials: GJ
authors.lastname: Yin
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Zhabei District, Shanghai 200072, China. sissifanyt@hotmail.com.
authors.forename: Yu Ting
authors.initials: YT
authors.lastname: Fan
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Zhabei District, Shanghai 200072, China. qiuleikaoyan@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Zhabei District, Shanghai 200072, China. caroline_1987@163.com.
authors.forename: Xiao Feng
authors.initials: XF
authors.lastname: Cang
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine, 100 Haining Road, Hongkou District, Shanghai 200080, China. medyuge@sina.com.
authors.forename: Ge
authors.initials: G
authors.lastname: Yu
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Zhabei District, Shanghai 200072, China. lillian_hyl@126.com.
authors.forename: Yan Ling
authors.initials: YL
authors.lastname: Hu
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Zhabei District, Shanghai 200072, China. xmiao0928@163.com.
authors.forename: Miao
authors.initials: M
authors.lastname: Xing
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Zhabei District, Shanghai 200072, China. sinnerwu1984@163.com.
authors.forename: De Qing
authors.initials: DQ
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine, 100 Haining Road, Hongkou District, Shanghai 200080, China. wangxingpeng@hotmail.com.
authors.forename: Xing Peng
authors.initials: XP
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Zhabei District, Shanghai 200072, China. huguoyongsh@sina.com.
authors.forename: Guo Yong
authors.initials: GY
authors.lastname: Hu
authors.affiliation: Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Zhabei District, Shanghai 200072, China. wanrong1970@163.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Wan
mesh_terms: D061354:Acinar Cells / Q000187:drug effects; D000681:Amylases / Q000097:blood; D000818:Animals; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D057888:Iridoid Glucosides / Q000494:pharmacology / Q000627:therapeutic use*; D008049:Lipase / Q000097:blood; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D019283:Pancreatitis, Acute Necrotizing / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013656:Taurocholic Acid / Q000633:toxicity; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D057888:Iridoid Glucosides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C078040:catalpol; D013656:Taurocholic Acid; D009195:Peroxidase; D008049:Lipase; D000681:Amylases