title: Fast ibuprofen, ketoprofen and naproxen simultaneous determination in human serum for clinical toxicology by GC-FID.
abstract: OBJECTIVE
  The aim of this study was to develop and validate a gas chromatographic method with flame ionization detection (GC-FID) for the measurement of ibuprofen, naproxen and ketoprofen for clinical toxicology purposes.
  
  
  METHODS
  100μL of plasma was treated with methyl chloroformate and derivatized analytes were extracted with hexane. Optimal conditions of the derivatization procedure have been found using the experimental chemometric design (face-centered central composite design). The selectivity and efficiency of the procedure was confirmed by GC-MS.
  
  
  RESULTS
  The assay was linear in the concentration range of 10-400μgmL(-1), with adequate accuracy and precision for GC-FID (98-106.7%, CV≤9.1%, respectively) and for GC-MS (99.3-105.5%, CV≤9.2%, respectively).
  
  
  CONCLUSIONS
  The entire sample preparation procedure is completed within 5 min and the quantitative results are available within 35 min. The method was successfully applied to quantify the selected compounds in serum of patients from emergency units.
authors.affiliation: Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic. Electronic address: tomas.hlozek@vfn.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Hložek
authors.affiliation: Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Bursová
authors.affiliation: Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Radomír
authors.initials: R
authors.lastname: Cabala
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D007052:Ibuprofen / Q000097:blood*; D007660:Ketoprofen / Q000097:blood*; D009288:Naproxen / Q000097:blood*
chemical_list: D009288:Naproxen; D007660:Ketoprofen; D007052:Ibuprofen
keywords: Chloroformate; Experimental design; GC; Non-steroidal anti-inflammatory drugs