title: [Selective medium to isolate human Bifidobacterium].
abstract: OBJECTIVE
  To compare five selective media to isolate human Bifidobacterium.
  
  
  METHODS
  Feces from six healthy human volunteers were diluted and cultivated on five Bifidobacterium selective media. After anaerobic cultivation, bacterial colonies were counted, selected and identified. Meanwhile, bacterial genomic DNA was extracted from the feces samples, and the Denaturing Gradient Gel Electrophoresis (DGGE) and Quantitative Polymerase Chain Reaction (q-PCR) were applied to reveal the diversity of Bifidobacterium.
  
  
  RESULTS
  The amount of Bifidobacterium grown on BSM and BLM media was similar to the result detected by q-PCR and was significantly higher than that on three other media. Bifidobacterium isolated from BLM medium was similar to the identified result of DGGE profile.
  
  
  CONCLUSIONS
  BLM medium is the best selective medium for Bifidobacterium isolation from human gastrointestinal tract.
authors.forename: Dongxue
authors.initials: D
authors.lastname: Huo
authors.forename: Jiachao
authors.initials: J
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Bai
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Huang
authors.forename: Heping
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000328:Adult; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D003470:Culture Media