title: Metabolic engineering of Bacillus amyloliquefaciens for poly-gamma-glutamic acid (γ-PGA) overproduction.
abstract: We constructed a metabolically engineered glutamate-independent Bacillus amyloliquefaciens strain with considerable γ-PGA production. It was carried out by double-deletion of the cwlO gene and epsA-O cluster, as well as insertion of the vgb gene in the bacteria chromosome. The final generated strain NK-PV elicited the highest production of γ-PGA (5.12 g l(-1)), which was 63.2% higher than that of the wild-type NK-1 strain (3.14 g l(-1)). The γ-PGA purity also improved in the NK-PV strain of 80.4% compared with 76.8% for the control. Experiments on bacterial biofilm formation experiment showed that NK-1 and NK-c (ΔcwlO) strains can form biofilm; the epsA-O deletion NK-7 and NK-PV strains could only form an incomplete biofilm.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin, China; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Gu
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.forename: Lifang
authors.initials: L
authors.lastname: Han
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Cao
authors.forename: Cunjiang
authors.initials: C
authors.lastname: Song
authors.forename: Weixia
authors.initials: W
authors.lastname: Gao
authors.forename: Shufang
authors.initials: S
authors.lastname: Wang
mesh_terms: D001407:Bacillus / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D018441:Biofilms / Q000254:growth & development; D017353:Gene Deletion; D060847:Metabolic Engineering; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D011995:Recombination, Genetic
chemical_list: C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid