title: Quantitative proteomic analysis of Huh-7 cells infected with Dengue virus by label-free LC-MS.
abstract: Dengue is an important and growing public health problem worldwide with an estimated 100million new clinical cases annually. Currently, no licensed drug or vaccine is available. During natural infection in humans, liver cells constitute one of the main targets of dengue virus (DENV) replication. However, a clear understanding of dengue pathogenesis remains elusive. In order to gain a better reading of the cross talk between virus and host cell proteins, we used a proteomics approach to analyze the host response to DENV infection in a hepatic cell line Huh-7. Differences in proteome expression were assayed 24h post-infection using label-free LC-MS. Quantitative analysis revealed 155 differentially expressed proteins, 64 of which were up-regulated and 91 down-regulated. These results reveal an important decrease in the expression of enzymes involved in the glycolytic pathway, citrate cycle, and pyruvate metabolism. This study provides large-scale quantitative information regarding protein expression in the early stages of infection that should be useful for better compression of the pathogenesis of dengue.
  
  
  CONCLUSIONS
  Dengue infection involves alterations in the homeostasis of the host cell. Defining the interactions between virus and cell proteins should provide a better understanding of how viruses propagate and cause disease. Here, we present for the first time the proteomic analysis of hepatocytes (Huh-7 cells) infected with DENV-2 by label-free LC-MS.
authors.affiliation: Instituto Nacional de Salud Pública, Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Universidad No. 655 Colonia Santa María Ahuacatitlán, Cerrada Los Pinos y Caminera, C.P. 62100, Cuernavaca, Mor., México.
authors.forename: Victoria
authors.initials: V
authors.lastname: Pando-Robles
authors.affiliation: Department of Pharmaceutical Chemistry, University of California at San Francisco, 600 16th Street, 94158-2517, San Francisco, CA, USA.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Oses-Prieto
authors.affiliation: Laboratorio Universitario de Proteómica (LUP), Instituto de Biotecnología-UNAM, Av. Universidad, 2001, Col Chamilpa, C.P. 62210, Cuernavaca, Mor., México.
authors.forename: Myriam
authors.initials: M
authors.lastname: Rodríguez-Gandarilla
authors.affiliation: Laboratorio Universitario de Proteómica (LUP), Instituto de Biotecnología-UNAM, Av. Universidad, 2001, Col Chamilpa, C.P. 62210, Cuernavaca, Mor., México.
authors.forename: Erika
authors.initials: E
authors.lastname: Meneses-Romero
authors.affiliation: Department of Pharmaceutical Chemistry, University of California at San Francisco, 600 16th Street, 94158-2517, San Francisco, CA, USA.
authors.forename: Alma L
authors.initials: AL
authors.lastname: Burlingame
authors.affiliation: Laboratorio Universitario de Proteómica (LUP), Instituto de Biotecnología-UNAM, Av. Universidad, 2001, Col Chamilpa, C.P. 62210, Cuernavaca, Mor., México. Electronic address: fbatista@ibt.unam.mx.
authors.forename: Cesar V F
authors.initials: CV
authors.lastname: Batista
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D003715:Dengue / Q000378:metabolism*; D003716:Dengue Virus; D015536:Down-Regulation; D005434:Flow Cytometry; D006019:Glycolysis; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D008099:Liver / Q000821:virology; D013058:Mass Spectrometry; D011506:Proteins / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D012984:Software; D015854:Up-Regulation; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: D011506:Proteins; D020543:Proteome; D014764:Viral Proteins
keywords: Dengue infection; Huh-7 cells; Label-free LC–MS