title: Effect of Mailuoning injection on 8-iso-prostaglandin F2 alpha and superoxide dismutase in rabbits with extremity ischemia-reperfusion injury.
abstract: BACKGROUND
  To date, there are no effective treatments for extremity ischemia-reperfusion (IR) injury. The objective of the present study was to explore the protective effect of Mailuoning on IR injury by investigating the plasma levels of 8-iso-prostaglandin F2 alpha (8-iso-PGF2α) and the activity of superoxide dismutase (SOD) in rabbits.
  
  
  METHODS
  The experimental models of posterior limb IR injury were established in thirty rabbits that were divided into three groups: the sham, IR, and IR + Mailuoning groups. At the end of ischemia, Mailuoning was injected intravenously into the rabbits in the IR + Mailuoning group, and normal saline solution was administered to the rabbits in the sham and IR groups. Venous blood samples were collected to measure the levels of 8-iso-PGF2α and the activity of SOD in the plasma at the following time points: at the onset of ischemia, the end of ischemia, and 2, 4, 8, 12, and 24 h after reperfusion. The skeletal muscles were harvested to examine the ultrastructure.
  
  
  RESULTS
  The levels of 8-iso-PGF2α increased significantly and SOD activity decreased in the IR group at every time point after reperfusion (P <0.01 or P <0.05). In contrast, the levels of 8-iso-PGF2α and SOD activity were not significantly different after reperfusion in the IR + Mailuoning group (P >0.05) but were significantly different compared with the IR group (P <0.01). Using electron microscopy, the skeletal muscle injury was shown to be milder in the IR+ Mailuoning group after reperfusion compared with the IR group.
  
  
  CONCLUSIONS
  The Mailuoning is capable of decreasing the excessive production of 8-iso-PGF2α and protecting SOD activity, thereby exhibiting a protective effect on extremity IR injury.
authors.affiliation: Department of Human Anatomy, Weifang Medical University, Weifang, Shandong, China. Electronic address: daijunwang@yahoo.com.
authors.forename: Dai-Jun
authors.initials: DJ
authors.lastname: Wang
authors.affiliation: School Hospital, Weifang Medical University, Weifang, Shandong, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
mesh_terms: D000818:Animals; D019610:Cytoprotection / Q000187:drug effects; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005121:Extremities / Q000098:blood supply; D015552:Injections, Intralesional; D008297:Male; D046529:Microscopy, Electron, Transmission; D018482:Muscle, Skeletal / Q000098:blood supply / Q000187:drug effects / Q000648:ultrastructure; D011817:Rabbits; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; C534919:mailuoning; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D013482:Superoxide Dismutase
keywords: 8-iso-prostaglandin F2 alpha; Ischemia–reperfusion injury; Mailuoning; Superoxide dismutase