title: Differential incorporation of glucose into biomass during Warburg metabolism.
abstract: It is well established that most cancer cells take up an increased amount of glucose relative to that taken up by normal differentiated cells. The majority of this glucose carbon is secreted from the cell as lactate. The fate of the remaining glucose carbon, however, has not been well-characterized. Here we apply a novel combination of metabolomic technologies to track uniformly labeled glucose in HeLa cancer cells. We provide a list of specific intracellular metabolites that become enriched after being labeled for 48 h and quantitate the fraction of consumed glucose that ends up in proteins, peptides, sugars/glycerol, and lipids.
authors.affiliation: Departments of †Chemistry, ‡Genetics, and §Medicine, Washington University in St. Louis , St. Louis, Missouri 63130, United States.
authors.forename: Ying-Jr
authors.initials: YJ
authors.lastname: Chen
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Huang
authors.forename: Nathaniel G
authors.initials: NG
authors.lastname: Mahieu
authors.forename: Kevin
authors.initials: K
authors.lastname: Cho
authors.forename: Jacob
authors.initials: J
authors.lastname: Schaefer
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Patti
mesh_terms: D002853:Chromatography, Liquid; D002952:Citric Acid Cycle; D005947:Glucose / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D050356:Lipid Metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D055442:Metabolome; D010427:Pentose Phosphate Pathway
chemical_list: D005947:Glucose