title: Antioxidant defense responses in Mytella guyanensis (Lamarck, 1819) exposed to an experimental diesel oil spill in Paranaguá Bay (Paraná, Brazil).
abstract: We evaluated the effects of diesel oil on the bivalve Mytella guyanensis using biomarkers of oxidative stress (glutathione S-transferase, glutathione peroxidase, and reduced glutathione) after an experimental in situ spill in a mangrove area in southern Brazil. A linear model was developed for the Multiple Before-After Control-Impact (MBACI) experimental design to assess the significance of biological responses. Control and impacted sites were sampled seven and two days before as well as two and seven days after the spill. With the exception of a late response of reduced glutathione (GSH) levels on day seven, none of the biomarkers were significantly altered by the impact. This result was attributed to the high environmental variability of the experimental sites combined with a low sensitivity of Mytella guyanensis to diesel oil at short time-scales. The high resistance of M. guyanensis suggests that its antioxidant response is triggered only after a medium- to long-term exposure to contaminants.
authors.affiliation: Laboratório de Bentos, Centro de Estudos do Mar, Universidade Federal do Paraná, Av. Beira Mar, s/n, CEP 83255-000 Pontal do Paraná, PR, Brazil. Electronic address: jmarques.oceano@gmail.com.
authors.forename: Joseane A
authors.initials: JA
authors.lastname: Marques
authors.affiliation: Departamento de Farmacologia, Universidade Federal do Paraná, Caixa Postal 19031, CEP 81531-970 Curitiba, PR, Brazil.
authors.forename: Helena C Silva
authors.initials: HC
authors.lastname: de Assis
authors.affiliation: Departamento de Farmacologia, Universidade Federal do Paraná, Caixa Postal 19031, CEP 81531-970 Curitiba, PR, Brazil.
authors.forename: Izonete C
authors.initials: IC
authors.lastname: Guiloski
authors.affiliation: Laboratório de Bentos, Centro de Estudos do Mar, Universidade Federal do Paraná, Av. Beira Mar, s/n, CEP 83255-000 Pontal do Paraná, PR, Brazil.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Sandrini-Neto
authors.affiliation: LABMAM/Departamento de Química, Pontifícia Universidade Católica do Rio de Janeiro, CEP 22453-900 Rio de Janeiro, Brazil.
authors.forename: Renato S
authors.initials: RS
authors.lastname: Carreira
authors.affiliation: Laboratório de Bentos, Centro de Estudos do Mar, Universidade Federal do Paraná, Av. Beira Mar, s/n, CEP 83255-000 Pontal do Paraná, PR, Brazil.
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Lana
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D061006:Bays; D015415:Biomarkers / Q000378:metabolism; D001938:Brazil; D005742:Gasoline / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D049879:Mytilidae / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D059392:Petroleum Pollution / Q000009:adverse effects*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005742:Gasoline; D011084:Polycyclic Aromatic Hydrocarbons; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase
keywords: Diesel oil; Field experiment; Mytella guyanensis; Oxidative stress; Paranaguá Bay