title: Facile synthesis of β-lactoglobulin-functionalized multi-wall carbon nanotubes and gold nanoparticles on glassy carbon electrode for electrochemical sensing.
abstract: A facile approach was developed for the preparation of nanocomposite based on β-lactoglobulin (BLG)-functionalized multi-wall carbon nanotubes (MWCNTs) and gold nanoparticles (GNPs) for the first time. Owing to the amphipathic nature, BLG can be adopted onto the surface of MWCNTs to form BLG-MWCNTs with uniform dispersion in water. Taking advantage of sulfhydryl groups on BLG-MWCNTs, GNPs were decorated on the BLG-MWCNTs-modified glassy carbon electrode (GCE) by electrodeposition. The nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy and X-ray spectroscopy analysis. Cyclic voltammetry and chronoamperometric method were used to evaluate the electrocatalytic ability of the nanocomposite. Furthermore, a glucose biosensor was developed based on the immobilization of glucose oxidase with cross-linking in the matrix of bovine serum albumin (BSA) on the nanocomposite modified GCE. The resulting biosensor exhibited high sensitivity (3.98 μA mM(-1)), wider linear range (0.025-5.5 mM), low detection limit (1.1 μM at the signal-to-noise ratio of 3) and fast response time (within 7s) for glucose detection.
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Du
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, PR China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Miao
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Acumox and Tuina, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, PR China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Fang
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Ma
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, PR China. Electronic address: qiangchen@nankai.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D002244:Carbon / Q000737:chemistry; D002417:Cattle; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D004867:Equipment Design; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000737:chemistry; D059629:Signal-To-Noise Ratio
chemical_list: D004800:Enzymes, Immobilized; D007782:Lactoglobulins; D037742:Nanotubes, Carbon; D012710:Serum Albumin, Bovine; D002244:Carbon; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose
keywords: Biosensor; Gold nanoparticles; Multi-wall carbon nanotube; Nanocomposite; β-Lactoglobulin