title: Polymorphisms of leptin-b gene associated with growth traits in orange-spotted grouper (Epinephelus coioides).
abstract: In mammals, leptin has been demonstrated to perform important roles in many physiological activities and to influence development, growth, metabolism and reproduction. However, in fish, its function is still unclear. Duplicate leptin genes, leptin-a and leptin-b, have been identified in the orange-spotted grouper. In the present study, the polymorphisms in the leptin-b gene of the orange-spotted grouper were detected, and the relation between these polymorphisms and 12 growth traits were analyzed. Six polymorphisms (including 3 single nucleotide polymorphisms (c.14G>A, c.93A>G, c.149G>A) in exon 1, 2 SNPs (c.181A>G, c.193G>A) in intron 1, and 1 SNP (c.360C>T) in exon 2) were identified and genotyped from 200 different individuals. The results revealed that the SNP c.149G>A was significantly associated with growth traits, that the heterozygous mutation genotype GA having negative effects on growth traits. However, the other five SNPs (c.14G>A, c.93A>G, c.181A>G, c.193G>A, c.360C>T) did not show significant associations with all the growth traits. Compared with our findings in leptin-a gene, the results suggested that the leptin-a hormone has more important physiological effects in fish bodies than the leptin-b type. Moreover, leptin genes were supposed to be one class of major candidate genes of regulating growth traits in the orange-spotted grouper.
authors.affiliation: Sanya Science & Technology Academy of Hainan National Breeding and Multiplication, Sanya 572000, China. huanghai74@126.com.
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory of Tropical Biology Resources, Ministry of Education, College of Ocean, Hainan University, Haikou 570228, China. 13780621366@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, China. mengzn@mail.sysu.edu.cn.
authors.forename: Zining
authors.initials: Z
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, China. lsszy@mail.sysu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, China. lsslxc@mail.sysu.edu.cn.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, China. zsdxgl@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Laboratory of Tropical Biology Resources, Ministry of Education, College of Ocean, Hainan University, Haikou 570228, China. luojian@hainu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Luo
authors.affiliation: Key Laboratory of Tropical Biology Resources, Ministry of Education, College of Ocean, Hainan University, Haikou 570228, China. chguh@hainu.edu.cn.
authors.forename: Guohua
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Tropical Biology Resources, Ministry of Education, College of Ocean, Hainan University, Haikou 570228, China. lsslhr@mail.sysu.edu.cn.
authors.forename: Haoran
authors.initials: H
authors.lastname: Lin
mesh_terms: D000818:Animals; D049628:Body Size / Q000235:genetics*; D020738:Leptin / Q000235:genetics*; D010473:Perciformes / Q000235:genetics* / Q000254:growth & development; D020641:Polymorphism, Single Nucleotide
chemical_list: D020738:Leptin