title: Chinese prescription Kangen-karyu and Salviae Miltiorrhizae Radix improve age-related oxidative stress and inflammatory response through the PI3K/Akt or MAPK pathways.
abstract: This study examined whether Kangen-karyu and its crude drug, Salviae Miltiorrhizae Radix, have a reno-protective effect on the age-related oxidative stress and inflammatory response through the phosphoinositide 3-kinase (PI3K)/Akt or mitogen-activated protein kinase (MAPK) pathways in aged rats. Kangen-karyu or Salviae Miltiorrhizae Radix (20 mg/kg body weight/day) was administered orally to old groups of rats for 16 days, and their effects were compared with the vehicle-treated old and young rats. The administration of Kangen-karyu caused a slight decrease in the serum glucose level and a significant decrease in the serum insulin level in the old rats. The increased levels of serum renal functional parameter (urea-nitrogen) and oxidative parameter were significantly reduced by both Kangen-karyu and Salviae Miltiorrhizae Radix. The old rats exhibited a dysregulation of the protein expression related to insulin resistance, oxidative stress, and inflammation in the kidneys, but Kangen-karyu administration significantly reduced the expression of the inflammatory proteins through the PI3K/Akt pathway. On the other hand, the Salviae Miltiorrhizae Radix-treated old rats showed a decrease in the inflammatory cytokines through the MAPK pathway. These results provide important evidence that Kangen-karyu and Salviae Miltiorrhizae Radix have a pleiotropic effect on the PI3K/Akt and MAPK pathways, showing renoprotective effects against the development of inflammation in old rats. This study provides scientific evidence that Kangen-karyu and Salviae Miltiorrhizae Radix improve the inflammatory responses via the PI3K/Akt or MAPK pathways in the kidney of old rats.
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA), Pusan National University, Geumjeong-gu, Busan 609-735, Korea.
authors.forename: Chan Hum
authors.initials: CH
authors.lastname: Park
authors.forename: Dae Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Min Hi
authors.initials: MH
authors.lastname: Park
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Kim
authors.forename: Nam Deuk
authors.initials: ND
authors.lastname: Kim
authors.forename: Cheol Min
authors.initials: CM
authors.lastname: Kim
authors.forename: Takashi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Takako
authors.initials: T
authors.lastname: Yokozawa
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Hyung Ryong
authors.initials: HR
authors.lastname: Moon
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000235:genetics*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000209:etiology* / Q000235:genetics* / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000378:metabolism; D007333:Insulin Resistance / Q000235:genetics; D007668:Kidney / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D008517:Phytotherapy; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza; D015398:Signal Transduction / Q000187:drug effects*; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators; D020928:Mitogen-Activated Protein Kinases; D016328:NF-kappa B; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D018808:Transcription Factor AP-1; C468270:Kangen-karyu; C028972:dan-shen root extract
keywords: Aging; Inflammation; Insulin Resistance; Kangen-karyu; Oxidative Stress; Renal Damage; Salviae Miltiorrhizae Radix