title: Embryonic miRNA profiles of normal and ectopic pregnancies.
abstract: Our objective was to investigate the miRNA profile of embryonic tissues in ectopic pregnancies (EPs) and controlled abortions (voluntary termination of pregnancy; VTOP). Twenty-three patients suffering from tubal EP and twenty-nine patients with a normal ongoing pregnancy scheduled for a VTOP were recruited. Embryonic tissue samples were analyzed by miRNA microarray and further validated by real time PCR. Microarray studies showed that four miRNAs were differentially downregulated (hsa-mir-196b, hsa-mir-30a, hsa-mir-873, and hsa-mir-337-3p) and three upregulated (hsa-mir-1288, hsa-mir-451, and hsa-mir-223) in EP compared to control tissue samples. Hsa-miR-196, hsa-miR-223, and hsa-miR-451 were further validated by real time PCR in a wider population of EP and control samples. We also performed a computational analysis to identify the gene targets and pathways which might be modulated by these three differentially expressed miRNAs. The most significant pathways found were the mucin type O-glycan biosynthesis and the ECM-receptor-interaction pathways. We also checked that the dysregulation of these three miRNAs was able to alter the expression of the gene targets in the embryonic tissues included in these pathways such as GALNT13 and ITGA2 genes. In conclusion, analysis of miRNAs in ectopic and eutopic embryonic tissues shows different expression patterns that could modify pathways which are critical for correct implantation, providing new insights into the understanding of ectopic implantation in humans.
authors.affiliation: Fundación IVI, Instituto Universitario IVI, INCLIVA, Valencia, Spain; INCLIVA Biomedical Research, Valencia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Dominguez
authors.affiliation: Fundación IVI, Instituto Universitario IVI, INCLIVA, Valencia, Spain.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Moreno-Moya
authors.affiliation: Hospital Universitario LaFe, Valencia, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Lozoya
authors.affiliation: Hospital Universitario LaFe, Valencia, Spain.
authors.forename: Ainhoa
authors.initials: A
authors.lastname: Romero
authors.affiliation: Fundación IVI, Instituto Universitario IVI, INCLIVA, Valencia, Spain.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Martínez
authors.affiliation: Hospital Universitario LaFe, Valencia, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Monterde
authors.affiliation: Hospital Universitario LaFe, Valencia, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Gurrea
authors.affiliation: Hospital Universitario LaFe, Valencia, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Ferri
authors.affiliation: Hospital Universitario LaFe, Valencia, Spain.
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Núñez
authors.affiliation: Fundación IVI, Instituto Universitario IVI, INCLIVA, Valencia, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
authors.affiliation: Fundación IVI, Instituto Universitario IVI, INCLIVA, Valencia, Spain; Hospital Universitario LaFe, Valencia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Pellicer
mesh_terms: D000029:Abortion, Legal; D000032:Abortion, Therapeutic; D000328:Adult; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000378:metabolism*; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D039421:Integrin alpha2 / Q000235:genetics / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D046228:Microarray Analysis; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis / Q000235:genetics; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D039421:Integrin alpha2; D035683:MicroRNAs; D011134:Polysaccharides; D017350:N-Acetylgalactosaminyltransferases; C000589774:GALNT13 protein, human