title: Analysis of oligosaccharides in beer using MALDI-TOF-MS.
abstract: Oligosaccharides in four different brands of beer (Cass, Hite, Budweiser, Miller) were systematically analysed with three different dihydroxybenzoic acid (DHB) isomer matrices (2,4-DHB, 2,5-DHB, and 2,6-DHB) using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS). Different experimental conditions, such as dilution (up to 1000-fold) and cationisation agents (sodium chloride or sodium trifluoroacetate) were analysed. No ionised peaks of oligosaccharides were observed with 2,4-DHB matrix. 2,6-DHB was more effective than 2,5-DHB in most of the investigated concentration ranges. 2,6-DHB with 4-fold dilution was the most effective. In certain cases, a cationisation agent was necessary to detect the signals of the oligosaccharides, and sodium chloride provided greater ionisation than sodium trifluoroacetate.
authors.affiliation: Department of Chemistry, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Eunhye
authors.initials: E
authors.lastname: Park
authors.forename: Hyojik
authors.initials: H
authors.lastname: Yang
authors.forename: Yangsun
authors.initials: Y
authors.lastname: Kim
authors.forename: Jeongkwon
authors.initials: J
authors.lastname: Kim
mesh_terms: D001515:Beer / Q000032:analysis*; D005841:Gentisates / Q000737:chemistry; D062385:Hydroxybenzoates / Q000737:chemistry; D007536:Isomerism; D009844:Oligosaccharides / Q000032:analysis*; D012965:Sodium Chloride / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014269:Trifluoroacetic Acid / Q000737:chemistry
chemical_list: D005841:Gentisates; D062385:Hydroxybenzoates; D009844:Oligosaccharides; D012965:Sodium Chloride; D014269:Trifluoroacetic Acid; C017704:beta-resorcylic acid; C412605:gamma-resorcylic acid; C010925:2,5-dihydroxybenzoic acid