title: The bioaccumulation and biotransformation of synthetic estrogen quinestrol in crucian carp.
abstract: The occurrence and fate of endocrine disrupting chemicals (EDCs) in aquatic species have attracted close attention during the last decades. In this study, the bioaccumulation and biotransformation of synthetic estrogen quinestrol, one of the typical EDCs, in the plasma and liver of crucian carp, were investigated by a newly developed and validated reversed-phase high performance liquid chromatography with fluorescent detection method. Crucian carp were exposed to quinestrol in concentration of 2, 10, 50, 100 μg/L (5.49, 27.43, 137.17, 274.34 nmol/L) for 60 days. After 60 days' exposure, the concentrations of quinestrol found in liver and plasma were in the range of 0.25-0.69 mg/kg and 0.19-0.30 mg/L respectively, positively correlated with the exposure concentrations ranged 2-100 μg/L (5.49-274.34 nmol/L). There was a negative correlation between the bio-accumulation ratios and the exposure concentrations of quinestrol. 17α-Ethinylestradiol was also found in liver and plasma, and the concentrations were 0.02-0.19 mg/kg and 0.37-0.96 mg/L, respectively. The results indicated that quinestrol can be accumulated and transformed to 17α-ethinylestradiol in crucian carp. Moreover, exposure to quinestrol caused oxidative damages to crucian carp and the content of malondialdehyde increased in all treatment concentrations.
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Geng
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China; College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, China.
authors.forename: Pingliang
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Deng
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Yongheng
authors.initials: Y
authors.lastname: Duan
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China. Electronic address: caoysong@126.com.
authors.forename: Yongsong
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D002347:Carps / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000378:metabolism*; D004967:Estrogens / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D011800:Quinestrol / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D004967:Estrogens; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol; D008315:Malondialdehyde; D011800:Quinestrol
keywords: 17α-ethinylestradiol; Bioaccumulation; Biotransformation; High-performance liquid chromatography; Malondialdehyde; Quinestrol