title: Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone.
abstract: Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering. However, such composites generally have no osteogenic activity per se. Here we report the synthesis of a chitosan-graft-polycaprolactone (CHS-g-PCL) and its subsequent characterization, including crystallinity, chemical structure and thermal stability. Upon surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein, protein adsorption, surface morphology and wettability were assessed. Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity. These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
authors.affiliation: Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg-University, Duesbergweg 6, D-55128 Mainz, Germany. Electronic address: wiens@uni-mainz.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Wiens
authors.affiliation: Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg-University, Duesbergweg 6, D-55128 Mainz, Germany.
authors.forename: Tarek A
authors.initials: TA
authors.lastname: Elkhooly
authors.affiliation: Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg-University, Duesbergweg 6, D-55128 Mainz, Germany.
authors.forename: Heinz-Christoph
authors.initials: HC
authors.lastname: Schröder
authors.affiliation: Institute for Functional Interfaces, Karlsruhe Institute of Technology, Germany.
authors.forename: Tawheed H A
authors.initials: TH
authors.lastname: Mohamed
authors.affiliation: Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg-University, Duesbergweg 6, D-55128 Mainz, Germany.
authors.forename: Werner E G
authors.initials: WE
authors.lastname: Müller
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods
chemical_list: D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D012822:Silicon Dioxide; D048271:Chitosan; D000469:Alkaline Phosphatase
keywords: Biosilica; Chitosan; Osteogenic activity; Polycaprolactone; Silicatein