title: Two-way regulation between cells and aligned collagen fibrils: local 3D matrix formation and accelerated neural differentiation of human decidua parietalis placental stem cells.
abstract: It has been well established that an aligned matrix provides structural and signaling cues to guide cell polarization and cell fate decision. However, the modulation role of cells in matrix remodeling and the feedforward effect on stem cell differentiation have not been studied extensively. In this study, we report on the concerted changes of human decidua parietalis placental stem cells (hdpPSCs) and the highly ordered collagen fibril matrix in response to cell-matrix interaction. With high-resolution imaging, we found the hdpPSCs interacted with the matrix by deforming the cell shape, harvesting the nearby collagen fibrils, and reorganizing the fibrils around the cell body to transform a 2D matrix to a localized 3D matrix. Such a unique 3D matrix prompted high expression of β-1 integrin around the cell body that mediates and facilitates the stem cell differentiation toward neural cells. The study offers insights into the coordinated, dynamic changes at the cell-matrix interface and elucidates cell modulation of its matrix to establish structural and biochemical cues for effective cell growth and differentiation.
authors.affiliation: Department of Biological and Chemical Sciences, Illinois Institute of Technology, 3101S Dearborn ST., Chicago, IL 60616, United States.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Biological and Chemical Sciences, Illinois Institute of Technology, 3101S Dearborn ST., Chicago, IL 60616, United States.
authors.forename: Bofan
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Obstetrics and Gynecology, University of Illinois at Chicago, 820 S Wood Street, M/C 808, Chicago, IL 60612, United States.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Strakova
authors.affiliation: Department of Biological and Chemical Sciences, Illinois Institute of Technology, 3101S Dearborn ST., Chicago, IL 60616, United States. Electronic address: wangr@iit.edu.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
mesh_terms: D002454:Cell Differentiation; D024042:Collagen Type I / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D006801:Humans; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology*; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology*
chemical_list: D024042:Collagen Type I
keywords: 3D matrix; Atomic force microscopy (AFM); Cell polarization; Collagen; Fibril alignment; Neural differentiation