title: Nanofibrous chitosan-polyethylene oxide engineered scaffolds: a comparative study between simulated structural characteristics and cells viability.
abstract: 3D nanofibrous chitosan-polyethylene oxide (PEO) scaffolds were fabricated by electrospinning at different processing parameters. The structural characteristics, such as pore size, overall porosity, pore interconnectivity, and scaffold percolative efficiency (SPE), were simulated by a robust image analysis. Mouse fibroblast cells (L929) were cultured in RPMI for 2 days in the presence of various samples of nanofibrous chitosan/PEO scaffolds. Cell attachments and corresponding mean viability were enhanced from 50% to 110% compared to that belonging to a control even at packed morphologies of scaffolds constituted from pores with nanoscale diameter. To elucidate the correlation between structural characteristics within the depth of the scaffolds' profile and cell viability, a comparative analysis was proposed. This analysis revealed that larger fiber diameters and pore sizes can enhance cell viability. On the contrary, increasing the other structural elements such as overall porosity and interconnectivity due to a simultaneous reduction in fiber diameter and pore size through the electrospinning process can reduce the viability of cells. In addition, it was found that manipulation of the processing parameters in electrospinning can compensate for the effects of packed morphologies of nanofibrous scaffolds and can thus potentially improve the infiltration and viability of cells.
authors.affiliation: Department of Materials Science, Tampere University of Technology, P.O. Box 589, 33101 Tampere, Finland.
authors.forename: Mohammad
authors.identifier: 0000-0002-9797-5634
authors.initials: M
authors.lastname: Kazemi Pilehrood
authors.affiliation: Department of Textile Engineering, Islamic Azad University South Tehran Branch, P.O. Box 11365-4435, Tehran, Iran.
authors.forename: Mandana
authors.initials: M
authors.lastname: Dilamian
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, Isfahan University of Medical Sciences, P.O. Box 81745-359, Isfahan, Iran.
authors.forename: Mina
authors.initials: M
authors.lastname: Mirian
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, Isfahan University of Medical Sciences, P.O. Box 81745-359, Isfahan, Iran.
authors.forename: Hojjat
authors.initials: H
authors.lastname: Sadeghi-Aliabadi
authors.affiliation: Department of Textile Engineering, Islamic Azad University South Tehran Branch, P.O. Box 11365-4435, Tehran, Iran.
authors.forename: Laleh
authors.initials: L
authors.lastname: Maleknia
authors.affiliation: Department of Materials Science, Tampere University of Technology, P.O. Box 589, 33101 Tampere, Finland.
authors.forename: Pertti
authors.identifier: 0000-0001-8718-8752
authors.initials: P
authors.lastname: Nousiainen
authors.affiliation: VTT Technical Research Centre of Finland, P.O. Box 1000, 02044 VTT, Finland.
authors.forename: Ali
authors.initials: A
authors.lastname: Harlin
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D051379:Mice; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry
chemical_list: D011092:Polyethylene Glycols; D048271:Chitosan