title: Human papillomavirus infection requires the TSG101 component of the ESCRT machinery.
abstract: Infection with human papillomaviruses (HPV) requires the minor capsid component L2, which plays an essential role in directing appropriate endosomal trafficking. Previous studies have indicated an infection route involving multi-vesicular bodies (MVBs), and an essential element in their biogenesis is the ESCRT machinery. Here we show that the ESCRT component TSG101 is required for optimal infection with both HPV-16 and BPV-1, with loss of TSG101 resulting in a decrease in viral infection, whereas overexpressed TSG101 increases rates of infection. We find that L2 proteins from multiple PV types interact with TSG101 and show that this interaction contributes to an alteration in the subcellular distribution of L2. In addition, TSG101 can modulate the levels of L2 polyubiquitination. These results demonstrate that TSG101 plays an important part in infection with diverse PVs, and suggests that trafficking of HPV through the ESCRT machinery and MVBs is part of infectious virus entry.
authors.affiliation: Department of Molecular Virology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Broniarczyk
authors.affiliation: Laboratory for Environmental Research, University of Nova Gorica, Nova Gorica, Slovenia.
authors.forename: Martina
authors.initials: M
authors.lastname: Bergant
authors.affiliation: Department of Molecular Virology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland. Electronic address: agjozef@amu.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Goździcka-Józefiak
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34149 Trieste, Italy. Electronic address: banks@icgeb.org.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Banks
mesh_terms: D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism*; D052162:Human papillomavirus 16 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism; D030361:Papillomavirus Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000099177:Tsg101 Protein
chemical_list: D036022:Capsid Proteins; D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D009856:Oncogene Proteins, Viral; D014157:Transcription Factors; D000099177:Tsg101 Protein; C063316:L2 protein, Human papillomavirus type 16
keywords: HPV; Infection; L2; TSG101