title: Influence of phenolic acids on indole acetic acid production and on the type III secretion system gene transcription in food-associated Pseudomonas fluorescens KM05.
abstract: The purpose of these investigations was to evaluate the reduction capability of phenolic acids (ferulic, chlorogenic, gallic, and p-coumaric acids) on indole acetic acid synthesis by food-associated Pseudomonas fluorescens KM05. Specific genetic primer for the type III secretion system (TTSS) in P. fluorescens KM05 was designed and the influence of phenolic acids on its expression was investigated. In the work the ferulic and chlorogenic acids at the concentration of 0.02 and 0.04 μg/ml affected on bacterial growth pattern and the signal molecules production. The phenolic acids, that were appreciable effective against P. fluorescens KM05 indole acetic acid production, significantly suppressed TTSS gene.
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, Poland. Electronic address: kmyszka@up.poznan.pl.
authors.forename: Kamila
authors.initials: K
authors.lastname: Myszka
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, Poland.
authors.forename: Marcin T
authors.initials: MT
authors.lastname: Schmidt
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, Poland.
authors.forename: Agnieszka K
authors.initials: AK
authors.lastname: Olejnik-Schmidt
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Leja
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Czaczyk
mesh_terms: D058947:Bacterial Secretion Systems / Q000187:drug effects* / Q000235:genetics*; D002726:Chlorogenic Acid / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology; D005516:Food Microbiology; D005707:Gallic Acid / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects* / Q000235:genetics; D062385:Hydroxybenzoates / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D010084:Oxidation-Reduction; D011422:Propionates; D011551:Pseudomonas fluorescens / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D014774:Virulence / Q000187:drug effects
chemical_list: D058947:Bacterial Secretion Systems; D003373:Coumaric Acids; D062385:Hydroxybenzoates; D007210:Indoleacetic Acids; D011422:Propionates; D002726:Chlorogenic Acid; D005707:Gallic Acid; C030737:indoleacetic acid; C004999:ferulic acid; C017616:phenolic acid; C495469:p-coumaric acid
keywords: Chlorogenic acid; Ferulic acid; Gallic acid; Indole acetic acid; Pseudomonas fluorescens; Type III secretion system; p-Coumaric acid