title: Aurora kinase A is not involved in CPEB1 phosphorylation and cyclin B1 mRNA polyadenylation during meiotic maturation of porcine oocytes.
abstract: Regulation of mRNA translation by cytoplasmic polyadenylation is known to be important for oocyte maturation and further development. This process is generally controlled by phosphorylation of cytoplasmic polyadenylation element binding protein 1 (CPEB1). The aim of this study is to determine the role of Aurora kinase A in CPEB1 phosphorylation and the consequent CPEB1-dependent polyadenylation of maternal mRNAs during mammalian oocyte meiosis. For this purpose, we specifically inhibited Aurora kinase A with MLN8237 during meiotic maturation of porcine oocytes. Using poly(A)-test PCR method, we monitored the effect of Aurora kinase A inhibition on poly(A)-tail extension of long and short cyclin B1 encoding mRNAs as markers of CPEB1-dependent cytoplasmic polyadenylation. Our results show that inhibition of Aurora kinase A activity impairs neither cyclin B1 mRNA polyadenylation nor its translation and that Aurora kinase A is unlikely to be involved in CPEB1 activating phosphorylation.
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
authors.forename: Pavla
authors.initials: P
authors.lastname: Komrskova
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
authors.forename: Andrej
authors.initials: A
authors.lastname: Susor
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Radek
authors.initials: R
authors.lastname: Malik
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Prochazkova
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Liskova
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Supolikova
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
authors.forename: Stepan
authors.initials: S
authors.lastname: Hladky
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Kubelka
mesh_terms: D000818:Animals; D064096:Aurora Kinase A / Q000378:metabolism*; D056744:Cyclin B1 / Q000235:genetics*; D005260:Female; D008540:Meiosis; D009865:Oocytes / Q000201:enzymology / Q000378:metabolism*; D010766:Phosphorylation; D026723:Polyadenylation; D012333:RNA, Messenger / Q000378:metabolism*; D034421:Sus scrofa / Q000378:metabolism; D039221:mRNA Cleavage and Polyadenylation Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D056744:Cyclin B1; D012333:RNA, Messenger; D039221:mRNA Cleavage and Polyadenylation Factors; D064096:Aurora Kinase A