title: [Time rhythm of homoharringtonine inducing K562 cell apoptosis and its mechanism].
abstract: This study was aimed to explore the change of K562 cell apoptosis at different time point after homoharringtonine (HHT) treatment and its mechanism. After treatment of K562 cells with 10 ng/ml HHT, the cell viability was tested with MTT assay; the expression of caspase-3 was detected with Western blot; the BCL-2 expression was analyzed with flow cytometry; the autophagosome was observed by electron microscopy. The results showed that the viability of K562 cells reduced gradually from day 1 to day 5 and ascended from day 6 to day 8 after HHT treatment. At the same time, the cleaved caspase-3 expression level of K562 cells increased gradually from day 1 to day 7, but reduced at the day 8 (P < 0.05). From day 1 to day 8 after HHT treatment, the BCL-2 expression level declined firstly and then went up (P < 0.05). Autophagosome was also seen remarkably at day 8 after HHT treatment. It is concluded that the apoptosis level of K562 cells after being treated with HHT enhances firstly and then declines , which may be associated with higher autophagy level in the late stage of HHT treatment.
authors.affiliation: Department of Hematology, Huai'an First People's Hospital, Nanjing Medical University, Huaian 223300, Jiangsu Province, China.
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Lu
authors.affiliation: Department of Hematology, Huai'an First People's Hospital, Nanjing Medical University, Huaian 223300, Jiangsu Province, China.
authors.forename: Wei-Ke
authors.initials: WK
authors.lastname: Cao
authors.affiliation: Department of Hematology, Huai'an First People's Hospital, Nanjing Medical University, Huaian 223300, Jiangsu Province, China.
authors.forename: Li-Lin
authors.initials: LL
authors.lastname: Ye
authors.affiliation: Department of Hematology, Huai'an First People's Hospital, Nanjing Medical University, Huaian 223300, Jiangsu Province, China.
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Deng
authors.affiliation: Department of Hematology, Huai'an First People's Hospital, Nanjing Medical University, Huaian 223300, Jiangsu Province, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Huai'an First People's Hospital, Nanjing Medical University, Huaian 223300, Jiangsu Province, China. E-mail: liyufeng99@netease.com.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D005434:Flow Cytometry; D006248:Harringtonines / Q000494:pharmacology*; D000077863:Homoharringtonine; D006801:Humans; D020014:K562 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism
chemical_list: D006248:Harringtonines; D019253:Proto-Oncogene Proteins c-bcl-2; D000077863:Homoharringtonine; C505407:CASP3 protein, human; D053148:Caspase 3