title: Targeted bioimaging and photodynamic therapy of cancer cells with an activatable red fluorescent bioprobe.
abstract: A new red-emissive bioprobe TPE-red-2AP2H was developed by taking advantage of the unique emission feature of tetraphenylethylene and a cancer cell-specific peptide. By responding to the target protein and the acidic microenvironment of tumor cells, activated fluorescence bioimaging was achieved with high signal-to-noise ratio and without involving mutiple washing steps. Apart from targeting the membrane-anchored LAPTM4B proteins, TPE-red-2AP2H was successfully utilized to trace the intracellular movement of LAPTM4B protein. The generation of (1)O2 under visible light irradiation makes this bioprobe also promising for targeted-photodynamic therapy. By discriminating the expression level of the target protein, TPE-red-2AP2H can respond to the progression status of tumors with different photodynamic therapy effect.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, CAS Key Laboratories of Organic Solids and Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Zhongguancun North First Street 2, Beijing 100190, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Hu
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Huang
authors.forename: Guanxin
authors.initials: G
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.forename: Deqing
authors.initials: D
authors.lastname: Zhang
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology*; D010778:Photochemotherapy; D026082:Singlet Oxygen / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005456:Fluorescent Dyes; D026082:Singlet Oxygen