title: Methane production in microbial reverse-electrodialysis methanogenesis cells (MRMCs) using thermolytic solutions.
abstract: The utilization of bioelectrochemical systems for methane production has attracted increasing attention, but producing methane in these systems requires additional voltage to overcome large cathode overpotentials. To eliminate the need for electrical grid energy, we constructed a microbial reverse-electrodialysis methanogenesis cell (MRMC) by placing a reverse electrodialysis (RED) stack between an anode with exoelectrogenic microorganisms and a methanogenic biocathode. In the MRMC, renewable salinity gradient energy was converted to electrical energy, thus providing the added potential needed for methane evolution from the cathode. The feasibility of the MRMC was examined using three different cathode materials (stainless steel mesh coated with platinum, SS/Pt; carbon cloth coated with carbon black, CC/CB; or a plain graphite fiber brush, GFB) and a thermolytic solution (ammonium bicarbonate) in the RED stack. A maximum methane yield of 0.60 ± 0.01 mol-CH4/mol-acetate was obtained using the SS/Pt biocathode, with a Coulombic recovery of 75 ± 2% and energy efficiency of 7.0 ± 0.3%. The CC/CB biocathode MRMC had a lower methane yield of 0.55 ± 0.02 mol-CH4/mol-acetate, which was twice that of the GFB biocathode MRMC. COD removals (89-91%) and Coulombic efficiencies (74-81%) were similar for all cathode materials. Linear sweep voltammetry and electrochemical impedance spectroscopy tests demonstrated that cathodic microorganisms enhanced electron transfer from the cathode compared to abiotic controls. These results show that the MRMC has significant potential for production of nearly pure methane using low-grade waste heat and a source of waste organic matter at the anode.
authors.affiliation: State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University , Beijing, 100084, Peoples' Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Luo
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Xia
authors.initials: X
authors.lastname: Huang
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Logan
mesh_terms: D001639:Bicarbonates / Q000737:chemistry*; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms / Q000254:growth & development; D002244:Carbon; D004560:Electricity; D004566:Electrodes; D005240:Feasibility Studies; D008697:Methane / Q000032:analysis*; D017014:Methanobacterium / Q000254:growth & development*; D010984:Platinum; D054712:Salinity; D012996:Solutions; D013193:Stainless Steel
chemical_list: D001639:Bicarbonates; D012996:Solutions; D013193:Stainless Steel; C027043:ammonium bicarbonate; D010984:Platinum; D002244:Carbon; D008697:Methane