title: Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites.
abstract: BACKGROUND
  Polyalthia longifolia Thwaites is an important traditional plant in India. Rutin, an active constituent has been reported to possess good amount of pharmacological as well as therapeutic potential.
  
  
  OBJECTIVE
  The aim of the present study was to find out by analytical techniques how much percentage of rutin is present in the plant leaves' ethanolic extract by analytical techniques.
  
  
  METHODS
  Shade dried leaves of Polyalthia longifolia were subjected to cold ethanolic extraction followed by monitoring the isolated rutin high-pressure liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) after carrying out preliminary phytochemical screening.
  
  
  RESULTS
  Extraction yield was found to be 13.94% w/w. Phytochemical screening of the extract showed the presence of flavonoids, steroids, diterpenoids, alkaloids, saponins, tannins and phenolic compounds and mucilage. From the Rf value, the ethanolic extract was found to be having constituent identical to rutin. By HPTLC and HPLC the amount of rutin was found to be 11.60% w/w and 4.03% w/v, respectively.
  
  
  CONCLUSIONS
  The active constituent isolated was found to be equal to rutin.
authors.affiliation: Department of Pharmacology and Pharmaceutical Medicinal Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India ; Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India.
authors.forename: Gaurav Mahesh
authors.initials: GM
authors.lastname: Doshi
authors.affiliation: Department of Pharmacology and Pharmaceutical Medicinal Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India.
authors.forename: Sandeep Prabhakar
authors.initials: SP
authors.lastname: Zine
authors.affiliation: Department of Pharmacology and Pharmaceutical Medicinal Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India.
authors.forename: Pratip Kashinath
authors.initials: PK
authors.lastname: Chaskar
authors.affiliation: Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabaugh, Aurangabad, Maharashtra, India.
authors.forename: Hemant Devidas
authors.initials: HD
authors.lastname: Une
keywords: High performance thin layer chromatography; High-pressure liquid chromatography; Polyalthia longifolia; Rutin; Thin layer chromatography