title: Deleted in liver cancer 1 expression and localization in hepatocellular carcinoma tissue sections.
abstract: The deleted in liver cancer (DLC) protein family is composed of proteins that are hypothesized to function predominantly by regulating the activity of the small GTPases. The aim of the present study was to determine the expression and exact localization of DLC1 in hepatocellular carcinoma (HCC) tissue sections. In two types of HCC tissues, typical and fibrolamellar, immunohistochemical and immunofluorescent analysis were performed to assess DLC1 immunoreactivity. Additionally, theDLC1gene status was determined by the fluorescencein situhybridization. According to the observations, DLC1 is often lost in cancer cells; however, it can remain within the stromal component of tumor sections. The DLC1 immunoreactivity was particularly noticeable within the capsules surrounding the tumor masses. It was found that theDLC1gene was deleted in 52% of HCC cases. In addition, the hemizygous deletion was observed to be independent of the HCC subtype. The results indicate that although the loss of DLC1 is a common step during hepatocarcinogenesis, this protein may be present in the tumor microenvironment.
authors.affiliation: Department of Pathomorphology, Medical University of Warsaw, Warsaw, Mazovia 02-091, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Wolosz
authors.affiliation: Laboratory of Molecular and Systemic Neuromorphology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Mazovia 02-093, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Walczak
authors.affiliation: Laboratory of Molecular and Systemic Neuromorphology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Mazovia 02-093, Poland.
authors.forename: Grzegorz M
authors.initials: GM
authors.lastname: Wilczynski
authors.affiliation: Department of Pathomorphology, Medical University of Warsaw, Warsaw, Mazovia 02-091, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Szparecki
authors.affiliation: Department of Pathomorphology, Medical University of Warsaw, Warsaw, Mazovia 02-091, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Wilczek
authors.affiliation: Department of Pathomorphology, Medical University of Warsaw, Warsaw, Mazovia 02-091, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Gornicka
keywords: deleted in liver cancer 1; fibrolamellar carcinoma; hepatocellular carcinoma