title: Ca(2+)-CaM regulating viability of Candida guilliermondii under oxidative stress by acting on detergent resistant membrane proteins.
abstract: Reactive oxygen species (ROS) play a vital role in reducing viability of yeast cells. The Ca(2+)-CaM signaling pathways are involved in regulating the intracellular ROS level in yeast cells under stress. Detergent resistant membranes (DRMs), the sterol-rich microdomains, participate in a wide range of cellular processes including growth, trafficking and death in yeast cells. In the present study, we found that Trifluoperazine (TFP), an antagonist of CaM, could increase the viability of Candida guilliermondii cells under H2O2 stress. Based on comparative analysis of DRM sub proteomics, a total number of 29 differentially expressed protein spots were identified, among which 8 protein spots belong to the electron transport chain and 7 protein spots belong to transporters. It is suggested that TFP treatment could modulate the intracellular ROS generation in yeast cells. We additionally ascertained that TFP treatment could effectively alleviate the ROS accumulation and protein damage in C. guilliermondii cells under H2O2 stress, via investigating the intracellular ROS levels and protein oxidative damage in yeast cells. These findings firstly revealed that the Ca(2+)-CaM signaling pathway is related to the viability of yeast cells under H2O2 stress, and provide novel evidences for exploring Ca(2+)-CaM's role in regulating this viability via acting on DRM proteins.
  
  
  CONCLUSIONS
  Detergent-resistant membranes (DRMs), which are more resistant to extraction with cold non-ionic detergents, have been considered the functional microdomains in the plasma membrane. In yeast, DRMs are involved in a wide range of additional cellular processes including cell growth and death. The Ca(2+)-CaM signaling pathways could regulate the stress tolerance of yeast cells by modulating the intracellular ROS generation. In this study, we found that trifluoperazine (TFP), a calmodulin antagonist, could increase the viability of C. guilliermondii under H2O2 stress. Based on comparative analysis of DRM sub proteomics, electron transport chain proteins and transporters were identified to be associated with the Ca(2+)-CaM transduction. We proved that TFP treatment decreases the intracellular ROS accumulation and alleviates oxidative damage to cellular proteins. These results ascertain that Ca(2+)-CaM is involved in regulating the viability of C. guilliermondii under oxidative stress via acting on the DRM proteins.
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Bang
authors.initials: B
authors.lastname: An
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Boqiang
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Guozheng
authors.initials: G
authors.lastname: Qin
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address: tsp@ibcas.ac.cn.
authors.forename: Shiping
authors.initials: S
authors.lastname: Tian
mesh_terms: D000932:Antiemetics / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology*; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002175:Candida / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D014268:Trifluoperazine / Q000494:pharmacology
chemical_list: D000932:Antiemetics; D002147:Calmodulin; D003902:Detergents; D005656:Fungal Proteins; D008565:Membrane Proteins; D014268:Trifluoperazine; D002118:Calcium
keywords: Biocontrol microbial agent; Ca(2+)-CaM; Detergent resistant membrane; Oxidative stress; Subproteomics