title: What's changed with genome editing?
abstract: Targeted genome editing using engineered nucleases can reliably generate defined genetic mutations, although the range of off-target lesions caused by these techniques is unclear. Recent papers in Cell Stem Cell perform high-coverage whole-genome sequencing to demonstrate the feasibility of obtaining correctly targeted pluripotent stem cell clones with minimal unintended modifications.
authors.affiliation: Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA; Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Shengdar Q
authors.initials: SQ
authors.lastname: Tsai
authors.affiliation: Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA; Department of Pathology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: jjoung@mgh.harvard.edu.
authors.forename: J Keith
authors.initials: JK
authors.lastname: Joung
mesh_terms: D002478:Cells, Cultured; D059766:DNA End-Joining Repair; D003851:Deoxyribonucleases / Q000378:metabolism*; D015894:Genome, Human; D055106:Genome-Wide Association Study; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D009154:Mutation / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000502:physiology*; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D003851:Deoxyribonucleases