title: A single immunization with recombinant rabies virus (ERAG3G) confers complete protection against rabies in mice.
abstract: New alternative bait rabies vaccines applicable to pet dogs and wild animals are needed to eradicate rabies in Korea. In this study, recombinant rabies virus, ERAG3G strain was constructed using reverse genetic system and the safety, efficacy and immunogenicity of the ERAG3G strain was evaluated in mice and dogs.
  
  
  
  Using the full-length genome mutated amino acid at position 333 of glycoprotein of rabies virus (RABV) and helper plasmids, the ERAG3G strain was rescued in BHK/T7-9 cells successfully. Mice were inoculated with the ERAG3G strain for safety and efficacy. Safety and immunogenicity of the dog inoculated with the ERAG3G strain (1 mL, 10(8.0) FAID50/mL) via intramuscular route was evaluated for 28 days after inoculation.
  
  
  
  The ERAG3G strain rescued by reverse genetic system was propagated well in the mouse neuroblastoma cells revealing titer of 10(8.5) FAID50/mL and was not pathogenic to 4- or 6-week-old mice that received by intramuscular or intracranical route. Immunization with the ERAG3G strain conferred complete protection from lethal RABV in mice. Dogs inoculated with the vaccine candidate via intramuscular route showed high neutralizing antibody titer ranging from 2.62 to 23.9 IU/mL at 28 days postinoculation.
  
  
  
  Our findings suggest that the ERAG3G strain plays an important role in inducing protective efficacy in mice and causes to arise anti-rabies neutralizing antibody in dogs.
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea.
authors.forename: Dong-Kun
authors.identifier: http://orcid.org/0000-0001-5765-3043
authors.initials: DK
authors.lastname: Yang
authors.affiliation: The United Graduated School of Veterinary Science, Gifu University, Gifu, Japan.
authors.forename: Keisuke
authors.identifier: http://orcid.org/0000-0002-6342-4738
authors.initials: K
authors.lastname: Nakagawa
authors.affiliation: The United Graduated School of Veterinary Science, Gifu University, Gifu, Japan.
authors.forename: Naoto
authors.identifier: http://orcid.org/0000-0003-0296-966X
authors.initials: N
authors.lastname: Ito
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea.
authors.forename: Ha-Hyun
authors.identifier: http://orcid.org/0000-0001-6473-0035
authors.initials: HH
authors.lastname: Kim
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea.
authors.forename: Bang-Hun
authors.identifier: http://orcid.org/0000-0002-3429-3425
authors.initials: BH
authors.lastname: Hyun
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea.
authors.forename: Jin-Ju
authors.identifier: http://orcid.org/0000-0002-5021-0941
authors.initials: JJ
authors.lastname: Nah
authors.affiliation: The United Graduated School of Veterinary Science, Gifu University, Gifu, Japan.
authors.forename: Makoto
authors.identifier: http://orcid.org/0000-0002-4300-893X
authors.initials: M
authors.lastname: Sugiyama
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea.
authors.forename: Jae-Young
authors.identifier: http://orcid.org/0000-0002-9554-9252
authors.initials: JY
authors.lastname: Song
keywords: Animals; Rabies virus; Recombinant rabies virus; Vaccine