title: Isolation and functional analysis of a glycolipid producing Rhodococcus sp. strain IITR03 with potential for degradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT).
abstract: A 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degrading bacterium strain IITR03 producing trehalolipid was isolated and characterized from a pesticides contaminated soil. The strain IITR03 was identified as a member of the genus Rhodococcus based on polyphasic studies. Under aqueous culture conditions, the strain IITR03 degraded 282 μM of DDT and could also utilize 10mM concentration each of 4-chlorobenzoic acid, 3-chlorobenzoic acid and benzoic acid as sole carbon and energy source. The catechol 1,2-dioxygenase enzyme activity resulted in conversion of catechol to form cis,cis-muconic acid. Cloning and sequencing of partial nucleotide sequence of catechol 1,2-dioxygenase gene (cat) from strain IITR03 revealed its similarity to catA gene present in Rhodococcus sp. strain Lin-2 (97% identity) and Rhodococcus strain AN22 (96% identity) degrading benzoate and aniline, respectively. The results suggest that the strain IITR03 could be useful for field bioremediation studies of DDT-residues and chlorinated aromatic compounds present in contaminated sites.
authors.affiliation: Environmental Biotechnology Division, CSIR-Indian Institute of Toxicology Research, Lucknow, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
authors.forename: Abhay
authors.initials: A
authors.lastname: Bajaj
authors.affiliation: MTCC-Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Mayilraj
authors.affiliation: Analytical Chemistry Section, CSIR-Indian Institute of Toxicology Research, Lucknow, India.
authors.forename: Mohana Krishna Reddy
authors.initials: MK
authors.lastname: Mudiam
authors.affiliation: Analytical Chemistry Section, CSIR-Indian Institute of Toxicology Research, Lucknow, India.
authors.forename: Devendra Kumar
authors.initials: DK
authors.lastname: Patel
authors.affiliation: Environmental Biotechnology Division, CSIR-Indian Institute of Toxicology Research, Lucknow, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. Electronic address: nmanickam@iitr.res.in.
authors.forename: Natesan
authors.initials: N
authors.lastname: Manickam
mesh_terms: D001673:Biodegradation, Environmental / Q000187:drug effects; D050548:Catechol 1,2-Dioxygenase / Q000235:genetics / Q000378:metabolism; D003634:DDT / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D019289:Pyruvic Acid / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012015:Reference Standards; D012240:Rhodococcus / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D019802:Succinic Acid / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D006017:Glycolipids; D012336:RNA, Ribosomal, 16S; D019289:Pyruvic Acid; D019802:Succinic Acid; D003634:DDT; D050548:Catechol 1,2-Dioxygenase
keywords: Biodegradation; Biosurfactant; Chloroaromatic compound; Rhodococcus sp.