title: Driving a planar model system into the 3(rd) dimension: generation and control of curved pore-spanning membrane arrays.
abstract: The generation of a regular array of micrometre-sized pore-spanning membranes that protrude from the underlying surface as a function of osmotic pressure is reported. Giant unilamellar vesicles are spread onto non-functionalized Si/SiO(2) substrates containing a highly ordered array of cavities with pore diameters of 850 nm, an interpore distance of 4 μm and a pore depth of 10 μm. The shape of the resulting pore-spanning membranes is controlled by applying an osmotic pressure difference between the bulk solution and the femtoliter-sized cavity underneath each membrane. By applying Young-Laplace's law assuming moderate lateral membrane tensions, the response of the membranes to the osmotic pressure difference can be theoretically well described. Protruded pore-spanning membranes containing the receptor lipid PIP(2) specifically bind the ENTH domain of epsin resulting in an enlargement of the protrusions and disappearance as a result of ENTH-domain induced defects in the membranes. These results are discussed in the context of an ENTH-domain induced reduction of lateral membrane tension and formation of defects as a result of helix insertion of the protein in the bilayer.
authors.affiliation: Institute of Organic and Biomolecular Chemistry, University of Göttingen, Tammannstrasse 2, 37077 Göttingen, Germany. csteine@gwdg.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Gleisner
authors.forename: Ingo
authors.initials: I
authors.lastname: Mey
authors.forename: Mariam
authors.initials: M
authors.lastname: Barbot
authors.forename: Christina
authors.initials: C
authors.lastname: Dreker
authors.forename: Michael
authors.initials: M
authors.lastname: Meinecke
authors.forename: Claudia
authors.initials: C
authors.lastname: Steinem
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000737:chemistry; D000818:Animals; D007477:Ions / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008422:Materials Testing; D008567:Membranes, Artificial; D018613:Microscopy, Confocal; D009995:Osmosis; D010713:Phosphatidylcholines / Q000737:chemistry; D011312:Pressure; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D051381:Rats; D012825:Silicon / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D007477:Ions; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D011506:Proteins; D012997:Solvents; D017971:Tin Compounds; C114154:epsin; C109984:indium tin oxide; D012822:Silicon Dioxide; C028694:1-palmitoyl-2-oleoylphosphatidylcholine; D012825:Silicon