title: Comparison ofK-rasmutations in lung, colorectal and gastric cancer.
abstract: K-rasis involved in the EGFR pathway that regulates cell survival, motility and proliferation, as well as angiogenesis and metastasis. It is also essential for carcinogenesis. TheK-rasmutation status can be used to predict the therapeutic efficacy of targeted drugs such as cetuximab. The aim of this study was to compareK-rasmutation in different types of cancer. Nested and COLD-PCR were used to detectK-rasmutations. The Chi-squared test was used for statistical analysis. In this study, the totalK-rasmutation frequency was found to be 9.09, 18.61 and 6.67% in lung, colorectal and gastric cancer, respectively. SimilarK-rasmutation frequencies were detected among sample types and genders for lung and gastric cancer, with the exception of colorectal cancer. However, age had no impact on theK-rasmutation rates.
authors.affiliation: Technical Department, Shanghai Shines Pharmaceuticals Company Limited, Research Base of Molecular Diagnosis Technology for Tumor Personalized Therapy, Development Center for Medical Science and Technology, Ministry of Health, Shanghai 201900, P.R. China.
authors.forename: Nanqiu
authors.initials: N
authors.lastname: Peng
authors.affiliation: Technical Department, Shanghai Shines Pharmaceuticals Company Limited, Research Base of Molecular Diagnosis Technology for Tumor Personalized Therapy, Development Center for Medical Science and Technology, Ministry of Health, Shanghai 201900, P.R. China.
authors.forename: Xintai
authors.initials: X
authors.lastname: Zhao
keywords: COLD-PCR; K-ras mutation; colorectal cancer; gastric cancer; lung cancer