title: Efficacy of verbenone and green leaf volatiles for protecting whitebark and limber pines from attack by mountain pine beetle (Coleoptera: Curculionidae: Scolytinae).
abstract: To develop safe and effective methods to protect whitebark pines, Pinus albicaulis Engelmann, and limber pines, Pinus flexilis James, from attack by mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), we compared verbenone and verbenone plus green leaf volatiles (GLVs) for prevention of beetle attack. We used two strategies: area-wide protection where semiochemical-releasing flakes are dispersed over the forest floor, and individual tree tests where flakes are applied to tree trunks. The area-wide bioassays were conducted by applying verbenone- and GLV-releasing flakes without stickers to the forest floor on 0.81-ha plots dominated by whitebark pines in the State of Washington with four replicates. We conducted individual tree bioassays by applying the same formulations with stickers to whitebark and limber pines in Montana and Colorado, respectively. In all three situations, both verbenone-alone and verbenone plus GLVs significantly increased the proportion of trees escaping mass attack by beetles, but the two formulations were not significantly different from one another. Despite a lack of significance at a Bonferroni-adjusted α = 0.05, adding GLVs gave slightly greater absolute levels of tree protection in most cases. Monitoring traps placed in the area-wide treatments in Washington showed similar outcomes for numbers of beetles trapped: both treatments had significantly fewer beetles than controls, and they were not significantly different from one another. At peak flight, however, plots with GLVs combined with verbenone had roughly 40% fewer beetles than plots with verbenone alone. GLVs are considerably cheaper than verbenone, so tests of higher application rates may be warranted to achieve enhanced tree protection at reasonable cost.
authors.affiliation: USDA Forest Service, Pacific SW Research Station, 800 Buchanan Street, Albany, CA 94710, USA.
authors.forename: Nancy E
authors.initials: NE
authors.lastname: Gillette
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: Kegley
authors.forename: Sheryl L
authors.initials: SL
authors.lastname: Costello
authors.forename: Sylvia R
authors.initials: SR
authors.lastname: Mori
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Webster
authors.forename: Constance J
authors.initials: CJ
authors.lastname: Mehmel
authors.forename: David L
authors.initials: DL
authors.lastname: Wood
mesh_terms: D000818:Animals; D000081008:Bicyclic Monoterpenes; D003120:Colorado; D007300:Insect Control / Q000379:methods*; D009009:Montana; D028223:Pinus / Q000254:growth & development; D018515:Plant Leaves; D013729:Terpenes / Q000494:pharmacology*; D055549:Volatile Organic Compounds / Q000494:pharmacology*; D014861:Washington; D048889:Weevils / Q000187:drug effects* / Q000502:physiology
chemical_list: D000081008:Bicyclic Monoterpenes; D013729:Terpenes; D055549:Volatile Organic Compounds; C052875:verbenone