title: Development and validation of an LC-MS method for determination of Karanjin in rat plasma: application to preclinical pharmacokinetics.
abstract: A selective and sensitive liquid chromatography-mass spectrometry (MS) method was developed and validated for the determination of karanjin in rat plasma. The target analyte, together with the internal standard (warfarin), was extracted from rat plasma by liquid-liquid extraction with ethyl acetate. Chromatographic separation was performed on a ZORBAX SB-C18 column using a mixture of acetonitrile and 0.1% aqueous formic acid as the mobile phase with linear gradient elution. MS detection was performed on a single quadrupole MS by selected ion monitoring mode via a positive electrospray ionization source. The assay exhibited a linear dynamic range of 2.50-3,000 ng/mL for karanjin. The intra- and inter-day precision was <10.8%, and the intra- and inter-day accuracy was <9.2%. The validated method has been applied to the preclinical pharmacokinetic studies of karanjin following oral administration of 5, 10 and 20 mg/kg karanjin to rats.
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai 264000, PR China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Yi
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai 264000, PR China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai 264000, PR China yilz_lukop@hotmail.com.
authors.forename: Longzhi
authors.initials: L
authors.lastname: Yi
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D001578:Benzopyrans; C052068:karanjin