title: [Imbalance in the expression of Foxp3, T-bet and GATA-3 is correlated with inflammation of chronic obstructive pulmonary disease].
abstract: OBJECTIVE
  To observe the change of regulatory T cells(Tregs), fork head-like transcription factor 3 (Foxp3), T box expressed in T cells (T-bet) and GATA binding protein 3 (GATA3) in rat models of chronic obstructive pulmonary disease (COPD).
  
  
  METHODS
  Thirty rats were randomly divided into control group and model group (n=15 each). The rats of model group were developed by lipopolysaccharide (LPS) and smoking. Interleukin-4 (IL-4) and γ-interferon (IFN-γ) in serum and bronchoalveolar lavage fluid (BALF) were detected 28 days after modeling by ELISA. Peripheral Tregs were detected by flow cytometry. The expressions of Foxp3, T-bet, GATA gene and protein in lung tissue were observed by reverse transcription PCR and Western blotting, respectively.
  
  
  RESULTS
  Compared with the control group, the model group had more serious inflammation in lung tissues, expressed the higher levels of IFN-γ in serum and BALF (P<0.01), T-bet mRNA and protein in lung tissue, and the lower levels of IL-4, CD4⁺ CD25⁺ Treg, CD4⁺ CD25⁺ Foxp3⁺ Treg (P<0.05), Foxp3, GATA-3 mRNA and protein (P<0.01). Correlation analysis showed that there were correlations between T-bet, GATA-3, Foxp3 expressions and IL-4, IFN-γ levels (P<0.05).
  
  
  CONCLUSIONS
  There is a relationship between inflammation and imbalance of in the expression of T-bet, GATA-3, Foxp3 in COPD.
authors.affiliation: Graduate School, Hubei University of Traditional Chinese Medicine, Wuhan 430065; Clinical College of Integrated Chinese and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.
authors.forename: Chengyang
authors.initials: C
authors.lastname: Wang
authors.affiliation: Clinical College of Integrated Chinese and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.
authors.forename: Xiangguo
authors.initials: X
authors.lastname: Liu
authors.affiliation: Clinical College of Integrated Chinese and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.
authors.forename: Qinghe
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Clinical College of Integrated Chinese and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.
authors.forename: Da
authors.initials: D
authors.lastname: Li
authors.affiliation: Clinical College of Integrated Chinese and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.
authors.forename: Qiaoxue
authors.initials: Q
authors.lastname: Ji
authors.affiliation: Department of Traditional Chinese Medicine, Second Affiliated Hospital, Anhui Medical University, Hefei 230601, China.
authors.forename: Chuanbo
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Respiration, First Affiliated Hospital, Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
authors.forename: Zegeng
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007249:Inflammation / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D007371:Interferon-gamma / Q000097:blood / Q000378:metabolism; D015847:Interleukin-4 / Q000097:blood / Q000378:metabolism; D008070:Lipopolysaccharides; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D029424:Pulmonary Disease, Chronic Obstructive / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism; D000098815:T-bet Transcription Factor
chemical_list: D051858:Forkhead Transcription Factors; C500522:Foxp3 protein, rat; D050990:GATA3 Transcription Factor; D008070:Lipopolysaccharides; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D015847:Interleukin-4; D007371:Interferon-gamma