title: Improvement in the repair of defects in maxillofacial soft tissue in irradiated minipigs by a mixture of adipose-derived stem cells and platelet-rich fibrin.
abstract: To find out if adipose-derived stem cells (ASC) and platelet-rich fibrin (PRF), alone or combined, had any effect on the repair of maxillofacial soft tissue defects in irradiated minipigs, ASC were isolated, characterised, and expanded. Twenty female minipigs, the right parotid glands of which had been irradiated, were randomly divided into 4 groups of 5 each: those in the first group were injected with both ASC and PRF (combined group), the second group was injected with ASC alone (ASC group), the third group with PRF alone (PRF group), and the fourth group with phosphate buffer saline (PBS) (control group). Six months after the last injection, the size and depth of each defect were assessed, and subcutaneous tissues were harvested, stained with haematoxylin and eosin, and examined immunohistologically and for apoptosis. Expanded cells were successfully isolated and identified. Six months after injection the defects in the 3 treated groups were significantly smaller (p<0.001) and shallower (p<0.001) than those in the control group. Those in the combined group were the smallest and shallowest. Haematoxylin and eosin showed that the 3 treated groups contained more subcutaneous adipose tissue than the control group, and also had significantly greater vascular density (p<0.001) and fewer apoptotic cells (p<0.001). Both ASC and PRF facilitate the repair of defects in maxillofacial soft tissue in irradiated minipigs, and their combined use is more effective than their use as single agents.
authors.affiliation: Department of Burns Surgery, Linyi People's Hospital, Linyi, Shandong 276000, China.
authors.forename: Yuanzheng
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Burns and Plastic Surgery, Dongguan People's Hospital, Dongguan, Guangdong 523059, China.
authors.forename: Zhanguo
authors.initials: Z
authors.lastname: Niu
authors.affiliation: Department of Ultrasonography, Linyi People's Hospital, Linyi, Shandong 276000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
authors.forename: Fukang
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Department of Burns Surgery, Linyi People's Hospital, Linyi, Shandong 276000, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Plastic Surgery, Linyi People's Hospital, Linyi, Shandong 276000, China. Electronic address: bainan2014@163.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Bai
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001792:Blood Platelets / Q000502:physiology*; D003094:Collagen / Q000032:analysis; D004547:Elastic Tissue / Q000473:pathology; D005260:Female; D005337:Fibrin / Q000627:therapeutic use*; D005355:Fibrosis; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000502:physiology; D010306:Parotid Gland / Q000293:injuries / Q000473:pathology / Q000528:radiation effects*; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000473:pathology / Q000628:therapy*; D011897:Random Allocation; D033581:Stem Cell Transplantation / Q000379:methods*; D040521:Subcutaneous Tissue / Q000293:injuries / Q000528:radiation effects; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology
chemical_list: D005337:Fibrin; D003094:Collagen
keywords: adipose-derived stem cells; platelet-rich fibrin; radiation defect; soft tissue defect