title: Development of a TaqMan-based real-time reverse transcription polymerase chain reaction assay for the detection of encephalomyocarditis virus.
abstract: Encephalomyocarditis virus (EMCV) is one of the major zoonosis pathogens and can cause acute myocarditis in young pigs or reproductive failure in sows. In this study, a TaqMan-based real-time reverse transcription polymerase chain reaction (RT-PCR) assay targeting 3D gene of EMCV was developed and their sensitivities and specificities were investigated. The results indicated that the standard curve had a wide dynamic range (10(1)-10(6) copies/μL) with a linear correlation (R(2)) of 0.996 between the cycle threshold (Ct) value and template concentration. The real-time RT-PCR assay is highly sensitive and able to detect 1.4×10(2) copies/μL of EMCV RNA, as no cross-reaction was observed with other viruses. These data suggested that the real-time RT-PCR assay developed in this study will be suitable for future surveillance and specific diagnosis of EMCV-infection.
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding 071001, Hebei, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, Hebei, China; North China Research Center of Animal Epidemic Pathogen Biology, China, Agriculture Ministry, Baoding 071001, Hebei, China.
authors.forename: Wanzhe
authors.initials: W
authors.lastname: Yuan
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding 071001, Hebei, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, Hebei, China; North China Research Center of Animal Epidemic Pathogen Biology, China, Agriculture Ministry, Baoding 071001, Hebei, China.
authors.forename: Yingshuai
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding 071001, Hebei, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, Hebei, China; North China Research Center of Animal Epidemic Pathogen Biology, China, Agriculture Ministry, Baoding 071001, Hebei, China.
authors.forename: Mingtan
authors.initials: M
authors.lastname: Sun
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding 071001, Hebei, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, Hebei, China; North China Research Center of Animal Epidemic Pathogen Biology, China, Agriculture Ministry, Baoding 071001, Hebei, China.
authors.forename: Xiuyuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding 071001, Hebei, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, Hebei, China; North China Research Center of Animal Epidemic Pathogen Biology, China, Agriculture Ministry, Baoding 071001, Hebei, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qi
authors.affiliation: College of Animal Medicine, Agricultural University of Hebei, Baoding 071001, Hebei, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, Hebei, China; North China Research Center of Animal Epidemic Pathogen Biology, China, Agriculture Ministry, Baoding 071001, Hebei, China. Electronic address: vaccine2000@126.com.
authors.forename: Jiguo
authors.initials: J
authors.lastname: Sun
mesh_terms: D000818:Animals; D018188:Cardiovirus Infections / Q000662:veterinary* / Q000821:virology; D003429:Cross Reactions; D004686:Encephalomyocarditis virus / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology*; D014730:Veterinary Medicine / Q000379:methods*
keywords: Detection; Encephalomyocarditis virus (EMCV); RT-PCR; Real-time; TaqMan