title: Genetic and component content differentiation between wild and cultivated populations of Paeonia lactiflora and related species used as Chishao and Baishao in China.
abstract: One hundred and forty-four samples of Chishao and Baishao, which represented six species of Paeonia L. were evaluated for their genetic variation, genetic differentiation and phylogenetic relationship, based on the nuclear ribosomal DNA internal transcribed spacer (ITS) region. Samples from representative of the population were then used to do a cultivation comparison experiment, and then to identify the contents of the active ingredients. The results showed there were differences in the haplotype distribution and frequency between populations of Chishao and Baishao. An analysis of molecular variance (AMOVA) indicated statistically significant (p<0.001) genetic differentiation between the populations of wild and cultivated Paeonia lactiflora PALL. The albiflorin content between Chishao and Baishao was also significantly different (p<0.05). All the results clearly illustrate that currently cultivated P. lactiflora cannot be used as a substitute for Chishao.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Wang
authors.forename: Peigen
authors.initials: P
authors.lastname: Xiao
authors.forename: Kun
authors.initials: K
authors.lastname: Luo
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Song
authors.forename: Shengli
authors.initials: S
authors.lastname: Wei
authors.forename: Zaiyou
authors.initials: Z
authors.lastname: Jian
authors.forename: Junling
authors.initials: J
authors.lastname: Hou
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.forename: Wenquan
authors.initials: W
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D019817:Benzoic Acid / Q000032:analysis; D001952:Bridged-Ring Compounds / Q000032:analysis; D002392:Catechin / Q000032:analysis; D002681:China; D018744:DNA, Plant / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005707:Gallic Acid / Q000032:analysis; D014644:Genetic Variation; D005960:Glucosides / Q000032:analysis; D006239:Haplotypes; D047348:Hydrolyzable Tannins / Q000032:analysis; D008969:Molecular Sequence Data; D039821:Monoterpenes / Q000032:analysis; D029596:Paeonia / Q000737:chemistry* / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D006027:Glycosides
chemical_list: D019817:Benzoic Acid; D001952:Bridged-Ring Compounds; D002392:Catechin; D018744:DNA, Plant; D004275:DNA, Ribosomal; D005707:Gallic Acid; D005960:Glucosides; D047348:Hydrolyzable Tannins; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin; C435084:pentagalloylglucose