title: A DNA minor groove binder shows high effectiveness as a quencher for FRET probes.
abstract: A non-fluorescent quencher based on thiazole orange was incorporated into oligonucleotides. Fluorimetry and fluorogenic real-time polymerase chain reaction experiments demonstrated that the quencher is effective for fluorescein amidite dyes. The thiazole orange quencher also increased the melting temperature of DNA duplexes, which may facilitate the design of shorter and more discriminatory probes. The effectiveness of the quencher in TaqMan probes was also demonstrated.
authors.affiliation: First Affiliated Hospital, School of Medicine, ZheJiang University, Qingchun Road 79, Hangzhou 310003, China.
authors.forename: Wei-Jia
authors.initials: WJ
authors.lastname: Fang
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Jin
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
authors.affiliation: HuiRui Biotechnology Company, Shanghai, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: First Affiliated Hospital, School of Medicine, ZheJiang University, Qingchun Road 79, Hangzhou 310003, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Gu
authors.affiliation: First Affiliated Hospital, School of Medicine, ZheJiang University, Qingchun Road 79, Hangzhou 310003, China. Electronic address: shusenzheng@zju.edu.cn.
authors.forename: Shu-Sen
authors.initials: SS
authors.lastname: Zheng
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D011804:Quinolines / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D052160:Benzothiazoles; D015342:DNA Probes; D005456:Fluorescent Dyes; D011804:Quinolines; C051000:thiazole orange; D004247:DNA
keywords: Fluorescein amidite; Non-fluorescent quencher; Thiazole orange