title: Platelet factor 4 protects kidney allograft in a rat kidney transplantation model.
abstract: Platelets are the cellular mediator of thrombosis, but it is becoming increasingly evident that platelets actively participate in inflammation and immune responses. A recent paper indicated that platelet factor 4 (PF4) alleviated cardiac allograft rejection in mice. But the role of PF4 on kidney transplantation has never been investigated. In our current experiment, PF4 administration alleviates immune responses to kidney transplantation. PF4 significantly alleviates vascular and glomerular changes, as well as interstitial inflammation, fibrosis, and tubular atrophy at day 56 after transplantation. PF4 decreases interleukin (IL)-17 production in vivo and also limits Th17 differentiation in vitro. Furthermore, the alleviated chronic vasculopathy and tubulointerstitial inflammation induced by PF4 were abolished with additional IL-17 administration. Meanwhile, decreased serum creatinine and urea induced by PF4 were also reversed by recombinant mouse IL-17 (rmIL-17). In conclusion, PF4 plays a protective role in chronic kidney allograft and this was associated with inhibition of IL-17 production.
authors.affiliation: Department of Urology, Capital Medical University of Beijing Friendship Hospital, Yongan Road 95# of Xicheng District, 100050, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Yichen
authors.initials: Y
authors.lastname: Zhu
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
mesh_terms: D064591:Allografts / Q000187:drug effects; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003404:Creatinine / Q000097:blood; D005355:Fibrosis / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control*; D006084:Graft Rejection / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control*; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology; D020381:Interleukin-17 / Q000096:biosynthesis / Q000627:therapeutic use; D016030:Kidney Transplantation; D007684:Kidney Tubules / Q000473:pathology; D008297:Male; D023421:Models, Animal; D009395:Nephritis, Interstitial / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control*; D010978:Platelet Factor 4 / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins / Q000627:therapeutic use; D058504:Th17 Cells / Q000166:cytology; D014184:Transplantation, Homologous; D014508:Urea / Q000097:blood
chemical_list: C515934:Il17a protein, rat; D020381:Interleukin-17; D011994:Recombinant Proteins; D010978:Platelet Factor 4; D014508:Urea; D003404:Creatinine