title: Construction of full-length cDNA library and development of EST-derived simple sequence repeat (EST-SSR) markers in Senecio scandens.
abstract: Senecio scandens Buch.-Ham. ex D. Don (Compositae) is a crucial source of Chinese traditional medicine with antibacterial properties. We constructed a cDNA library and obtained expressed sequence tags (ESTs) to show the distribution of gene ontology annotations for mRNAs, using an individual plant with superior antibacterial characteristics. Analysis of comparative genomics indicates that the putative uncharacterized proteins (21.07%) might be derived from "molecular function unknown" clones or rare transcripts. Furthermore, the Compositae had high cross-species transferability of EST-derived simple sequence repeats (EST-SSR), based on valid amplifications of 206 primer pairs developed from the newly assembled expressed sequence tag sequences in Artemisia annua L. Among those EST-SSR markers, 52 primers showed polymorphic amplifications between individuals with contrasting diverse antibacterial traits. Our sequence data and molecular markers will be cost-effective tools for further studies such as genome annotation, molecular breeding, and novel transcript profiles within Compositae species.
authors.affiliation: Department of Cell Biology and Genetics, Zunyi Medical College, 201 Dalian Road, Zunyi, 563003, Guizhou, China, qiangang69@sina.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Qian
authors.forename: Junjiao
authors.initials: J
authors.lastname: Ping
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Delin
authors.initials: D
authors.lastname: Xu
mesh_terms: D019659:Asteraceae / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D020224:Expressed Sequence Tags; D015723:Gene Library; D005819:Genetic Markers; D005822:Genetic Vectors; D018895:Microsatellite Repeats
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D005819:Genetic Markers