title: Comparison of five different RNA sources to examine the lactating bovine mammary gland transcriptome using RNA-Sequencing.
abstract: The objective of this study was to examine five different sources of RNA, namely mammary gland tissue (MGT), milk somatic cells (SC), laser microdissected mammary epithelial cells (LCMEC), milk fat globules (MFG) and antibody-captured milk mammary epithelial cells (mMEC) to analyze the bovine mammary gland transcriptome using RNA-Sequencing. Our results provide a comparison between different sampling methods (invasive and non-invasive) to define the transcriptome of mammary gland tissue and milk cells. This information will be of value to investigators in choosing the most appropriate sampling method for different research applications to study specific physiological states during lactation. One of the simplest procedures to study the transcriptome associated with milk appears to be the isolation of total RNA directly from SC or MFG released into milk during lactation. Our results indicate that the SC and MFG transcriptome are representative of MGT and LCMEC and can be used as effective and alternative samples to study mammary gland expression without the need to perform a tissue biopsy.
authors.affiliation: Department of Animal Science, University of California-Davis, One Shields Ave., Davis, 95616, CA, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Cánovas
authors.affiliation: Department of Animal Science, University of California-Davis, One Shields Ave., Davis, 95616, CA, USA.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Rincón
authors.affiliation: Institut National de la Recherche Agronomique, UMR 1313 Génétique animale et Biologie intégrative, F-78350 Jouy-en-Josas, France.
authors.forename: Claudia
authors.initials: C
authors.lastname: Bevilacqua
authors.affiliation: Department of Animal Science, University of California-Davis, One Shields Ave., Davis, 95616, CA, USA.
authors.forename: Alma
authors.initials: A
authors.lastname: Islas-Trejo
authors.affiliation: Institut National de la Recherche Agronomique, UMR 1313 Génétique animale et Biologie intégrative, F-78350 Jouy-en-Josas, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Brenaut
authors.affiliation: Department of Animal Science, University of California-Davis, One Shields Ave., Davis, 95616, CA, USA.
authors.forename: Russell C
authors.initials: RC
authors.lastname: Hovey
authors.affiliation: INRA, AGROCAMPUS OUEST, UMR1348 PEGASE, F-35590 Saint-Gilles, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Boutinaud
authors.affiliation: Institut National de la Recherche Agronomique, UMR 1313 Génétique animale et Biologie intégrative, F-78350 Jouy-en-Josas, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Morgenthaler
authors.affiliation: Department of Animal Science, University of California-Davis, One Shields Ave., Davis, 95616, CA, USA.
authors.forename: Monica K
authors.initials: MK
authors.lastname: VanKlompenberg
authors.affiliation: 1] Institut National de la Recherche Agronomique, UMR 1313 Génétique animale et Biologie intégrative, F-78350 Jouy-en-Josas, France [2].
authors.forename: Patrice
authors.initials: P
authors.lastname: Martin
authors.affiliation: 1] Department of Animal Science, University of California-Davis, One Shields Ave., Davis, 95616, CA, USA [2].
authors.forename: Juan F
authors.initials: JF
authors.lastname: Medrano
mesh_terms: D000465:Algorithms; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D007774:Lactation / Q000502:physiology*; D008321:Mammary Glands, Animal / Q000502:physiology*; D008892:Milk; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome / Q000502:physiology*
chemical_list: D014157:Transcription Factors; D012313:RNA