title: Inactivated Sendai virus strain Tianjin induces apoptosis in human breast cancer MDA-MB-231 cells.
abstract: Sendai virus strain Tianjin is a novel genotype. Here, we investigate the antitumor and proapoptotic effects of ultraviolet-inactivated Sendai virus strain Tianjin (UV-Tianjin) on human breast cancer MDA-MB-231 cells in vitro, as well as the involvement of the apoptotic pathway in the mechanism of UV-Tianjin-induced antitumor effects. MTT assays showed that treatment with UV-Tianjin dose-dependently inhibited the proliferation of MDA- MB-231 cells but not normal MCF 10A breast epithelium cells. Hoechst staining and flow cytometric analysis revealed that UV-Tianjin induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Moreover, UV-Tianjin treatment resulted in reduction in the mitochondria membrane potential (MMP) and release of cytochrome complex (cyt c) via regulation of Bax and Bcl-2, as well as activation of caspase-9, caspase-3, Fas, FasL and caspase-8 in MDA-MB-231 cells. In summary, our study suggests that UV-Tianjin exhibits anticancer activity in human breast cancer MDA-MB-231 cells through inducing apoptosis, which may involve both the endogenous mitochondrial and exogenous death receptor pathways.
authors.affiliation: Department of Microbiology, Basic Medical College, Tianjin Medical University, Tianjin, China E-mail : sly.good@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Han
authors.initials: H
authors.lastname: Han
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Xiao-Zhu
authors.initials: XZ
authors.lastname: Xu
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Shi
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000276:immunology / Q000473:pathology*; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D029082:Sendai virus / Q000502:physiology* / Q000528:radiation effects; D014407:Tumor Cells, Cultured; D038301:Virus Inactivation / Q000528:radiation effects