title: Mapping of polar fox renal cortex proteins using two-dimensional gel electrophoresis and mass spectrometry--a preliminary study.
abstract: The aim of the present study was to establish protein map of polar fox (Alopex lagopus) renal cortex. Kidney cortex proteins of isoelectric point ranging from 3 to 10 were analysed using two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Sixteen protein spots corresponding to thirteen different gene products were identified. These proteins were divided into following groups: lipid and fatty acid metabolism, amino acid metabolism, energetic pathways, regulatory proteins, transport proteins and structural proteins. This is the first attempt to create reproducible 2-D map, of renal cortex proteins characteristic for polar foxes, used as animal model for carnivores. It is worth emphasizing that the results of this study may broaden currently available protein databases.
authors.forename: A K
authors.initials: AK
authors.lastname: Ciechanowicz
authors.forename: M
authors.initials: M
authors.lastname: Ozgo
authors.forename: Ł R
authors.initials: ŁR
authors.lastname: Stański
authors.forename: A
authors.initials: A
authors.lastname: Herosimczyk
authors.forename: A
authors.initials: A
authors.lastname: Piotrowska
authors.forename: R
authors.initials: R
authors.lastname: Szymeczko
authors.forename: M
authors.initials: M
authors.lastname: Laszczyńska
authors.forename: W F
authors.initials: WF
authors.lastname: Skrzypczak
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary*; D005589:Foxes / Q000145:classification* / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D007672:Kidney Cortex / Q000378:metabolism*; D013058:Mass Spectrometry / Q000662:veterinary*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D059467:Transcriptome / Q000502:physiology
chemical_list: D011506:Proteins