title: Water Balance, Hormone Homeostasis, and Sugar Signaling Are All Involved in Tomato Resistance to Tomato Yellow Leaf Curl Virus.
abstract: Vacuolar water movement is largely controlled by membrane channels called tonoplast-intrinsic aquaporins (TIP-AQPs). Some TIP-AQP genes, such as TIP2;2 and TIP1;1, are up-regulated upon exposure to biotic stress. Moreover, TIP1;1 transcript levels are higher in leaves of a tomato (Solanum lycopersicum) line resistant to Tomato yellow leaf curl virus (TYLCV) than in those of a susceptible line with a similar genetic background. Virus-induced silencing of TIP1;1 in the tomato resistant line and the use of an Arabidopsis (Arabidopsis thaliana) tip1;1 null mutant showed that resistance to TYLCV is severely compromised in the absence of TIP1:1. Constitutive expression of tomato TIP2;2 in transgenic TYLCV-susceptible tomato and Arabidopsis plants was correlated with increased TYLCV resistance, increased transpiration, decreased abscisic acid levels, and increased salicylic acid levels at the early stages of infection. We propose that TIP-AQPs affect the induction of leaf abscisic acid, which leads to increased levels of transpiration and gas exchange, as well as better salicylic acid signaling.
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Dagan
authors.initials: D
authors.lastname: Sade
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Nir
authors.initials: N
authors.lastname: Sade
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Oz
authors.initials: O
authors.lastname: Shriki
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Stephen
authors.initials: S
authors.lastname: Lerner
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Alem
authors.initials: A
authors.lastname: Gebremedhin
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Asaf
authors.initials: A
authors.lastname: Karavani
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Yariv
authors.initials: Y
authors.lastname: Brotman
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Sonia
authors.initials: S
authors.lastname: Osorio
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Lothar
authors.initials: L
authors.lastname: Willmitzer
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.).
authors.forename: Henryk
authors.initials: H
authors.lastname: Czosnek
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel (D.S., N.S., O.S., S.L., A.G., A.K., H.C., M.M.); andMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany (Y.B., S.O., A.R.F., L.W.) moshelio@agri.huji.ac.il.
authors.forename: Menachem
authors.initials: M
authors.lastname: Moshelion