title: Crystallization and preliminary analysis of the NqrA and NqrC subunits of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae.
abstract: The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio cholerae is a membrane protein complex consisting of six different subunits NqrA-NqrF. The major domains of the NqrA and NqrC subunits were heterologously expressed in Escherichia coli and crystallized. The structure of NqrA1-377 was solved in space groups C222₁ and P2₁ by SAD phasing and molecular replacement at 1.9 and 2.1 Å resolution, respectively. NqrC devoid of the transmembrane helix was co-expressed with ApbE to insert the flavin mononucleotide group covalently attached to Thr225. The structure was determined by molecular replacement using apo-NqrC of Parabacteroides distasonis as search model at 1.8 Å resolution.
authors.affiliation: Institute for Neuropathology, University of Freiburg, Breisacher Strasse 64, 79106 Freiburg, Germany.
authors.forename: Georg
authors.initials: G
authors.lastname: Vohl
authors.affiliation: Department of Chemistry and Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, Konstanz, 78457, Germany.
authors.forename: Ruslan
authors.initials: R
authors.lastname: Nedielkov
authors.affiliation: Hermann-Staudinger-Graduate School, University of Freiburg, Hebelstrasse 27, 79104 Freiburg, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Claussen
authors.affiliation: Institute for Neuropathology, University of Freiburg, Breisacher Strasse 64, 79106 Freiburg, Germany.
authors.forename: Marco S
authors.initials: MS
authors.lastname: Casutt
authors.affiliation: Department of Microbiology, University of Hohenheim, 70599 Garbenstrasse 30, Stuttgart, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Vorburger
authors.affiliation: Department of Chemistry and Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, Konstanz, 78457, Germany.
authors.forename: Kay
authors.initials: K
authors.lastname: Diederichs
authors.affiliation: Department of Chemistry and Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, Konstanz, 78457, Germany.
authors.forename: Heiko M
authors.initials: HM
authors.lastname: Möller
authors.affiliation: Department of Microbiology, University of Hohenheim, 70599 Garbenstrasse 30, Stuttgart, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Steuber
authors.affiliation: Institute for Neuropathology, University of Freiburg, Breisacher Strasse 64, 79106 Freiburg, Germany.
authors.forename: Günter
authors.initials: G
authors.lastname: Fritz
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005486:Flavin Mononucleotide / Q000737:chemistry*; D015870:Gene Expression; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011808:Quinone Reductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040681:Structural Homology, Protein; D014734:Vibrio cholerae / Q000737:chemistry* / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D005486:Flavin Mononucleotide; C084142:sodium-translocating NADH-quinone reductase; D011808:Quinone Reductases
keywords: Na+-translocating NQR; Vibrio cholerae; covalently bound FMN