title: Occurrence, genetic characterization and antimicrobial resistance of Salmonella isolated from chicken meat and giblets.
abstract: SUMMARY This study was undertaken to survey the presence of Salmonella in 200 chicken samples collected from Mansoura, Egypt. Salmonella was detected in 16% (8/50), 28% (14/50), 32% (16/50) and 60% (30/50) of whole chicken carcasses, drumsticks, livers and gizzards, respectively, with an overall prevalence of 34% (68/200) among all samples. One hundred and sixty-six isolates were identified biochemically as Salmonella, and confirmed genetically by PCR, based on the presence of invA and stn genes. The spvC gene, however, was detected in only 25.3% (42/166) of the isolates. Isolates were serotyped as Salmonella Enteritidis (37.3%), S. Typhimurium (30.1%), S. Kentucky (10.8%), S. Muenster (8.4%), S. Virchow (4.8%), S. Anatum (4.8%), S. Haifa (1.2%), and four were non-typable. Antimicrobial susceptibility tests of the Salmonella isolates revealed that 100% were resistant to each of erythromycin, penicillin, and amoxicillin, while 98.8%, 96.4%, 95.2%, and 91.6% were resistant to nalidixic acid, sulphamethoxazole, oxytetracycline, and ampicillin, respectively. Multidrug resistance was evident for 92.8% of the isolates. The high contamination level of chicken meat with multidrug-resistant Salmonella can constitute a problem for public health.
authors.affiliation: Department of Food Hygiene and Control, Faculty of Veterinary Medicine,Mansoura University,Mansoura,Egypt.
authors.forename: S M
authors.initials: SM
authors.lastname: Abd-Elghany
authors.affiliation: Department of Food Hygiene and Control, Faculty of Veterinary Medicine,Mansoura University,Mansoura,Egypt.
authors.forename: K I
authors.initials: KI
authors.lastname: Sallam
authors.affiliation: Department of Food Hygiene and Control, Faculty of Veterinary Medicine,Mansoura University,Mansoura,Egypt.
authors.forename: A
authors.initials: A
authors.lastname: Abd-Elkhalek
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST),Tsukisamu-Higashi, Toyohira-ku, Sapporo,Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tamura
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D019757:Carbon-Oxygen Lyases / Q000235:genetics; D002645:Chickens / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D012475:Salmonella / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012477:Salmonella enteritidis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004768:Enterotoxins; D037521:Virulence Factors; C082864:enterotoxin, Salmonella; C075518:invA protein, Bacteria; D019757:Carbon-Oxygen Lyases; C527194:phosphothreonine lyase, Salmonella enterica
keywords: virulence genes