title: Ferrocenylnaphthalene diimide-based electrochemical detection of aberrant methylation in hTERT gene.
abstract: Since aberrant methylation at CpG sites is linked to the silencing of tumor suppressor genes, DNA methylation analysis is important for cancer diagnosis. We developed ferrocenylnaphthalene diimide (FND), which has two ferrocenyl moieties at the substituent termini, as an electrochemical indicator for hybridized DNA duplexes. In this study, we attempted to detect aberrant methylation of human telomerase reverse transcriptase gene (hTERT), an efficient cancer marker, using FND-based hybridization coupled with electrochemical detection via a multi-electrode chip.
authors.affiliation: Department of Applied Chemistry, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu, Fukuoka, 804-8550, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sato
authors.forename: Toshiro
authors.initials: T
authors.lastname: Saeki
authors.forename: Tomoki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kanezaki
authors.forename: Mana
authors.initials: M
authors.lastname: Hayakawa
authors.forename: Kazuya
authors.initials: K
authors.lastname: Haraguchi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kodama
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Nishihara
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tominaga
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000302:isolation & purification; D015374:Biosensing Techniques; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D006801:Humans; D007094:Imides / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics; D019098:Telomerase / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D014408:Biomarkers, Tumor; D005296:Ferrous Compounds; D007094:Imides; C406363:ferrocenylnaphthalene diimide; C509186:TERT protein, human; D019098:Telomerase