title: Picking vanished proteins from the void: how to collect and ship/share extremely dilute proteins in a reproducible and highly efficient manner.
abstract: Successful proteome analyses of highly dilute samples are strongly dependent on optimized workflows considering especially sample preparation prior to highly sensitive mass spectrometric analysis. Various methods are available for enrichment of proteome samples, each characterized by specific advantages and disadvantages limiting their general application as a method of choice. Here we suggest an optimized universal protocol ensuring reproducibility and effective enrichment of dilute samples by commercial affinity beads. By comparably assessing the performance of the new protocol with selected standard enrichment techniques, we show the seamless application of the enrichment in common mass spectrometry based proteomic workflows. Further, novel applications are suggested including a facile storage and shipping of desiccated, trapped proteome samples at ambient temperatures and usage of the affinity beads for gel-free proteomic approaches.
authors.affiliation: Institute for Microbiology, Ernst-Moritz-Arndt University Greifswald , Friedrich-Ludwig-Jahn-Strasse 15, 17487 Greifswald, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Bonn
authors.forename: Jürgen
authors.initials: J
authors.lastname: Bartel
authors.forename: Knut
authors.initials: K
authors.lastname: Büttner
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
authors.forename: Andreas
authors.initials: A
authors.lastname: Otto
authors.forename: Dörte
authors.initials: D
authors.lastname: Becher
mesh_terms: D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D011506:Proteins