title: Peroxiredoxin 5 from common cutworm (Spodoptera litura) acts as a potent antioxidant enzyme.
abstract: In this study, we describe the cloning and characterization of a Prx from the common cutworm Spodoptera litura (SlPrx5). The SlPrx5 cDNA contains an open reading frame of 477 bp encoding a predicted protein of 159 amino acid residues, 16.902 kDa, and an isoelectric point of 7.68. Furthermore, the deduced amino acid sequence of the SlPrx5 cDNA showed 86% identity to Papilio xuthus Prx5, 72% to Aedes aegypti Prx5, and 64-67% to other insect Prxs. A phylogenetic analysis further revealed that the deduced amino acid sequence of SlPrx5 groups within the atypical 2-Cys Prx cluster. Recombinant SlPrx5 (20 kDa) purified from baculovirus-infected insect cells was found to reduce H2O2 in the presence of electrons donated by dithiothreitol and protect super-coiled DNA from damage by metal-catalyzed oxidation in vitro. During S. litura development, SlPrx5 is constitutively expressed in the epidermis, fat body, and midgut, with the highest expression occurring in the sixth-instar larval stage in the fat body and midgut. Additionally, SlPrx5 mRNA expression was up-regulated after injection with H2O2, cumene hydroperoxide, indoxacarb, and metaflumizone. A disc diffusion assay indicated that recombinant SlPrx5 can play a functional role in protecting cells from oxidative stress in vivo. These results provide insight into the role of SlPrx5 during development and the oxidative stress response of S. litura.
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Hu
authors.initials: H
authors.lastname: Wan
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Tinghao
authors.initials: T
authors.lastname: Kang
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Sha
authors.initials: S
authors.lastname: Zhan
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: You
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Fuxing
authors.initials: F
authors.lastname: Zhu
authors.affiliation: College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Shangdong Zibo Tobacco Co. LTD, Zibo 255035, PR China.
authors.forename: Haigang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China; College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea. Electronic address: brjin@dau.ac.kr.
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Jin
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: jianhl@mail.hzau.edu.cn.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001483:Base Sequence; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D011994:Recombinant Proteins; D054464:Peroxiredoxins
keywords: Antioxidant enzyme; Common cutworm; Oxidative stress; Peroxiredoxin; Spodoptera litura