title: Identification of genomic locus responsible for experimentally induced testicular teratoma 1 (ett1) on mouse Chr 18.
abstract: Spontaneous testicular teratomas (STTs) composed by various kinds of tissues are derived from primordial germ cells (PGCs) in the fetal testes of the mouse. In contrast, intra-testicular grafts of the mouse strain (129/Sv-Ter (+/+)) fetal testes possessed the ability to develop the experimental testicular teratomas (ETTs), indistinguishable from the STTs at a morphological level. In this study, linkage analysis was performed for exploration of possible candidate genes involving in ETT development using F2 intercross fetuses derived from [LTXBJ × 129/Sv-Ter (+/+)] F1 hybrids. Linkage analysis with selected simple sequence length polymorphisms along chromosomes 18 and 19, which have been expected to contain ETT-susceptibility loci, demonstrated that a novel recessive candidate gene responsible for ETT development is located in 1.1 Mb region between the SSLP markers D18Mit81 and D18Mit184 on chromosome 18 in the 129/Sv-Ter (+/+) genetic background. Since this locus is different from the previously known loci (including Ter, pgct1, and Tgct1) for STT development, we named this novel gene "experimental testicular teratoma 1 (ett1)". To resolve the location of ett1 independently from other susceptibility loci, ett1 loci was introduced in a congenic strain in which the distal segment of chromosome 18 in LTXBJ strain mice had been replaced by a 1.99 Mbp genomic segment of the 129/Sv-Ter (+/+) mice. Congenic males homozygous for the ett1 loci were confirmed to have the ability to form ETTs, indicating that this locus contain the gene responsible for ETTs. We listed candidate genes included in this region, and discussed about their possible involvement in induction of ETTs.
authors.affiliation: Biological Science Course, Graduate School of Science, National University Corporation Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8529, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Ikue
authors.initials: I
authors.lastname: Kubo
authors.forename: Saeri
authors.initials: S
authors.lastname: Suganuma
authors.forename: Nastumi
authors.initials: N
authors.lastname: Fujita
authors.forename: Makiko
authors.initials: M
authors.lastname: Itakura
authors.forename: Tae
authors.initials: T
authors.lastname: Hayashi
authors.forename: Shuji
authors.initials: S
authors.lastname: Takabayashi
authors.forename: Hideki
authors.initials: H
authors.lastname: Katoh
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ohira
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sato
authors.forename: Motoko
authors.initials: M
authors.lastname: Noguchi
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Tokumoto
mesh_terms: D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics*; D005260:Female; D056426:Genetic Loci; D020022:Genetic Predisposition to Disease; D008297:Male; D057507:Mice, 129 Strain; D011110:Polymorphism, Genetic; D013724:Teratoma / Q000235:genetics* / Q000473:pathology; D013736:Testicular Neoplasms / Q000235:genetics* / Q000473:pathology; D013737:Testis / Q000196:embryology / Q000473:pathology