title: Trimetazidine significantly reduces cerulein-induced pancreatic apoptosis.
abstract: OBJECTIVE
  Acute pancreatitis continues to be associated with significant rates of mortality and morbidity, and therapeutic options are still very limited. We aimed to investigate the efficacy of trimetazidine on cerulein-induced pancreatic apoptosis and histopathological and biochemistrical consequences of acute pancreatitis.
  
  
  METHODS
  Thirty-two Wistar albino rats were randomized into four groups (group 1: control group; group 2: acute pancreatitis group; group 3: acute pancreatitis and trimetazidine treatment group; group 4: placebo group). Acute edematous pancreatitis was induced by subcutaneous cerulein injection (20 μg/kg) four times at one-hour intervals. Trimetazidine was prepared in suspension form. In group 3, after gas anesthesia, trimetazidine was administrated to rats via a catheter. Serum interleukin (IL)-1β, tumor necrosis factor (TNF)-α, amylase, lipase and leukocyte levels, pancreatic apoptotic status and pancreatic Schoenberg scores were determined for all groups. Results are given as the mean ± SD. A value of P<0.05 was accepted as statistically significant. SPSS for Windows v15.0 was used for statistical analyses.
  
  
  RESULTS
  In the acute pancreatitis group IL-1β, amylase, lipase and leukocyte levels were elevated and pancreatic histopathological evaluation revealed a diagnosis of acute pancreatitis IL-1β amylase and lipase levels and pancreatic inflammation were decreased significantly in the trimetazidine group (P<0.01). White blood cell counts and TNF-α concentrations for the trimetazidine group and the acute pancreatitis group were not significantly different. Trimetazidine significantly reduced apoptosis in pancreatic tissues and Schoenberg scores were also significantly reduced (P<0.05).
  
  
  CONCLUSIONS
  In this study, we showed that trimetazidine treatment significantly decreases the levels of IL-1β, amylase and lipase reduces pancreatic apoptosis and ameliorates the histopathological findings of cerulein-induced acute pancreatitis. Trimetazidine could be a new therapeutic option in the early treatment of acute pancreatitis.
authors.affiliation: GATA Haydarpasa Training Hospital, Gastroenterology Department, 34668 Uskudar-Istanbul, Turkey. Electronic address: alpaslantanoglu@yahoo.com.
authors.forename: Alpaslan
authors.initials: A
authors.lastname: Tanoglu
authors.affiliation: GATA Haydarpasa Training Hospital, Gastroenterology Department, 34668 Uskudar-Istanbul, Turkey. Electronic address: yusufyazgan@gmail.com.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Yazgan
authors.affiliation: GATA Haydarpasa Training Hospital, Gastroenterology Department, 34668 Uskudar-Istanbul, Turkey. Electronic address: dr_mustafakaplan@yahoo.com.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Kaplan
authors.affiliation: GATA Haydarpasa Training Hospital, Pathology Department, 34668 Uskudar-Istanbul, Turkey. Electronic address: patologub@gmail.com.
authors.forename: Ufuk
authors.initials: U
authors.lastname: Berber
authors.affiliation: GATA Haydarpasa Training Hospital, Gastroenterology Department, 34668 Uskudar-Istanbul, Turkey. Electronic address: drmuammerkara70@hotmail.com.
authors.forename: Muammer
authors.initials: M
authors.lastname: Kara
authors.affiliation: GATA Haydarpasa Training Hospital, Pathology Department, 34668 Uskudar-Istanbul, Turkey. Electronic address: demireldilaver@yahoo.com.
authors.forename: Dilaver
authors.initials: D
authors.lastname: Demırel
authors.affiliation: GATA Haydarpasa Training Hospital, Biochemistry Department, 34668 Uskudar-Istanbul, Turkey. Electronic address: osmetip@yahoo.com.
authors.forename: Osman Metin
authors.initials: OM
authors.lastname: Ipcioglu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002108:Ceruletide / Q000494:pharmacology*; D004195:Disease Models, Animal; D008297:Male; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014292:Trimetazidine / Q000627:therapeutic use*
chemical_list: D002108:Ceruletide; D014292:Trimetazidine