title: IL-10 signalling blockade at the time of immunization inhibits Human papillomavirus 16 E7 transformed TC-1 tumour cells growth in mice.
abstract: IL-10 signalling blockade by intra-peritoneal injection of anti-IL-10 receptor antibodies at the time of immunization enhances vaccine induced CD8+ T cell responses and promotes bacteria, parasitic and viral control. We now show that blockade of IL-10 signalling at the time of immunization enhances vaccine induced antigen specific CD8+ T cell responses to both dominant and subdominant CTL epitopes. Injection of anti-IL-10 receptor antibodies subcutaneous at the time of immunization also enhances CD8+ T cell responses. Furthermore, IL-10 signalling blockade at the time of a Human papillomavirus 16 E7 peptide/LPS immunization, prevents HPV16 E7 transformed TC-1 tumour growth in mice. Immunization in the presence of anti-IL-10R antibodies and Monophosphoryl lipid A, generates antigen specific CD8+ T cell responses similar to immunization with LPS. Our results suggest that immunization and IL-10 signalling blockade may provide a novel way for the development of therapeutic vaccines against cancer.
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Chen
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China.
authors.forename: Xiongfei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Wu
authors.affiliation: Division of Molecular and Gene Therapies, Griffith Health Institute and School of Medical Science, Griffith University, Gold Coast, QLD 4215, Australia.
authors.forename: Ming Q
authors.initials: MQ
authors.lastname: Wei
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China; School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of Sunshine Coast, Locked Bag 4, Marcoochydore DC, QLD 4558, Australia. Electronic address: xiaosongl@yahoo.com.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Cancer Research Institute, Foshan First People's Hospital, Foshan, Guangdong 528000, China. Electronic address: wyjian@fsyyy.com.
authors.forename: Yuejian
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics; D006801:Humans; D007114:Immunization; D016753:Interleukin-10 / Q000037:antagonists & inhibitors* / Q000276:immunology; D008050:Lipid A / Q000031:analogs & derivatives / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000276:immunology*; D053918:Papillomavirus Vaccines / Q000276:immunology*; D053701:Receptors, Interleukin-10 / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000517:prevention & control / Q000628:therapy
chemical_list: D000906:Antibodies; C556062:IL10 protein, mouse; D008050:Lipid A; D008070:Lipopolysaccharides; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; D053701:Receptors, Interleukin-10; C059731:oncogene protein E7, Human papillomavirus type 16; D016753:Interleukin-10; C048436:monophosphoryl lipid A
keywords: Cervical cancer; IL10; Immunotherapy