title: Prediction of pregnancy viability in bovine in vitro-produced embryos and recipient plasma with Fourier transform infrared spectroscopy.
abstract: We analyzed embryo culture medium (CM) and recipient blood plasma using Fourier transform infrared (FTIR) metabolomics to predict pregnancy outcome. Individually cultured, in vitro-produced (IVP) blastocysts were transferred to recipients as fresh and vitrified-warmed. Spent CM and plasma samples were evaluated using FTIR. The discrimination capability of the classifiers was assessed for accuracy, sensitivity (pregnancy), specificity (nonpregnancy), and area under the receiver operator characteristic curve (AUC). Within all IVP fresh embryos (birth rate=52%), high AUC were obtained at birth, especially with expanded blastocysts (CM: 0.80±0.053; plasma: 0.89±0.034). The AUC of vitrified IVP embryos (birth rate = 31%) were 0.607±0.038 (CM, expanded blastocysts) and 0.672±0.023 (plasma, all stages). Recipient plasma generally predicted pregnancy outcome better than did embryo CM. Embryos and recipients with improved pregnancy viability were identified, which could increase the economic benefit to the breeding industry.
authors.affiliation: Centro de Biotecnología Animal-Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Muñoz
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, 310 Cedar street, LSOG 304B, New Haven, CT 06520; Department of Computer Engineering, Okan University, Tuzla Kampusu, 34959, Tuzla, Istanbul, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Uyar
authors.affiliation: Centro de Biotecnología Animal-Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Correia
authors.affiliation: Centro de Biotecnología Animal-Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Díez
authors.affiliation: Servicios Científico Técnicos (Oviedo), Universidad de Oviedo, 33006 Oviedo, Asturias, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Fernandez-Gonzalez
authors.affiliation: Centro de Biotecnología Animal-Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain.
authors.forename: J N
authors.initials: JN
authors.lastname: Caamaño
authors.affiliation: U.T.E. Bos-Lugar de Bos, Guísamo 15640 Bergondo, A Coruña, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Martínez-Bello
authors.affiliation: Centro de Biotecnología Animal-Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Trigal
authors.affiliation: Swedish University of Agricultural Sciences, PO Box 7054, SE75007 Uppsala, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Humblot
authors.affiliation: Union Nationale des Cooperatives D' Elevage et Insemination Animale (UNCEIA), Department of Research and Development, 13 rue Jouet, 94704 Maisons Alfort, France.
authors.forename: C
authors.initials: C
authors.lastname: Ponsart
authors.affiliation: UNCEIA, Station Expérimentale, 484 Chemin Darefin, 38300 Chateauvillain, France.
authors.forename: C
authors.initials: C
authors.lastname: Guyader-Joly
authors.affiliation: Centro de Biotecnología Animal-Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Carrocera
authors.affiliation: Centro de Biotecnología Animal-Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Martin
authors.affiliation: Union Nationale des Cooperatives D' Elevage et Insemination Animale (UNCEIA), Department of Research and Development, 13 rue Jouet, 94704 Maisons Alfort, France.
authors.forename: B
authors.initials: B
authors.lastname: Marquant Le Guienne
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, 310 Cedar street, LSOG 304B, New Haven, CT 06520.
authors.forename: E
authors.initials: E
authors.lastname: Seli
authors.affiliation: Centro de Biotecnología Animal-Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain. Electronic address: egomez@serida.org.
authors.forename: E
authors.initials: E
authors.lastname: Gomez
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002417:Cattle; D015925:Cryopreservation / Q000379:methods; D003470:Culture Media; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D055432:Metabolomics; D008954:Models, Biological; D010949:Plasma; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary*; D011270:Pregnancy, Animal; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003470:Culture Media
keywords: bovine; embryo; pregnancy; recipient