title: A modular tyrosine kinase deoxyribozyme with discrete aptamer and catalyst domains.
abstract: We assess the utility of integrating a predetermined aptamer DNA module adjacent to a random catalytic DNA region for identifying new deoxyribozymes by in vitro selection. By placing a known ATP aptamer next to an N40 random region, an explicitly modular DNA catalyst for tyrosine side chain phosphorylation is identified. The results have implications for broader identification of deoxyribozymes that function with small-molecule substrates.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA. scott@scs.illinois.edu.
authors.forename: Victor
authors.initials: V
authors.lastname: Dokukin
authors.forename: Scott K
authors.initials: SK
authors.lastname: Silverman
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D020134:Catalytic Domain; D021881:DNA, Catalytic / Q000737:chemistry*; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; D011505:Protein-Tyrosine Kinases