title: Enzymatic degradation of Elephant grass (Pennisetum purpureum) stems: influence of the pith and bark in the total hydrolysis.
abstract: The internal pith of a high energy plant, Elephant grass (EG), was more extensively degraded (>50% dry matter) compared to the outer cortex (31%) or the whole stem (35%) by an enzyme preparation from Humicola insolens, Ultraflo. Reducing sugars and acetic acid release from the pith was also higher compared to the cortex. Supplementation of Ultraflo with a type-C feruloyl esterase increased the level of deacetylation but also led to reduced solubilisation. The addition of 20% dimethyl sulfoxide (DMSO) as a co-solvent also reduced the solubility of EG by Ultraflo, although acetic acid release was increased, complimenting previous results found on model substrates. The presence of DMSO was also shown to have a protective effect on xylanase activity but not acetyl esterase activity in Ultraflo. Xylan in the biomass was preferentially solubilised by DMSO, while Ultraflo removed more glucose than xylose.
authors.affiliation: Centro de Investigaciones Biológicas, Campus Universidad, Ramiro de Maeztu 9, 28040 Madrid, Spain. Electronic address: mpboada@cib.csic.es.
authors.forename: Marta
authors.initials: M
authors.lastname: Pérez-Boada
authors.affiliation: Centro de Investigaciones Biológicas, Campus Universidad, Ramiro de Maeztu 9, 28040 Madrid, Spain. Electronic address: aliprieto@cib.csic.es.
authors.forename: Alicia
authors.initials: A
authors.lastname: Prieto
authors.affiliation: Department of Plant Biotechnology, Instituto de Recursos Naturales y Agrobiológicas de Sevilla, CSIC, Sevilla, Spain. Electronic address: p.l.k.prinsen@uva.nl.
authors.forename: Pepijn
authors.initials: P
authors.lastname: Prinsen
authors.affiliation: INRA, UMR 1163 Biotechnologie des Champignons Filamenteux, 163 avenue de Luminy, 13288 Marseille cedex 09, France; Aix-Marseille Université, POLYTECH Marseille, UMR 1163 Biotechnologie des Champignons Filamenteux, 163 avenue de Luminy, 13288 Marseille cedex 09, France. Electronic address: marie-pierre.forquin@univ-amu.fr.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Forquin-Gomez
authors.affiliation: Department of Plant Biotechnology, Instituto de Recursos Naturales y Agrobiológicas de Sevilla, CSIC, Sevilla, Spain. Electronic address: delrio@irnase.csic.es.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: del Río
authors.affiliation: Department of Plant Biotechnology, Instituto de Recursos Naturales y Agrobiológicas de Sevilla, CSIC, Sevilla, Spain. Electronic address: anagu@irnase.csic.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Gutiérrez
authors.affiliation: Centro de Investigaciones Biológicas, Campus Universidad, Ramiro de Maeztu 9, 28040 Madrid, Spain. Electronic address: atmartinez@cib.csic.es.
authors.forename: Ángel T
authors.initials: ÁT
authors.lastname: Martínez
authors.affiliation: Centro de Investigaciones Biológicas, Campus Universidad, Ramiro de Maeztu 9, 28040 Madrid, Spain; INRA, UMR 1163 Biotechnologie des Champignons Filamenteux, 163 avenue de Luminy, 13288 Marseille cedex 09, France; Aix-Marseille Université, POLYTECH Marseille, UMR 1163 Biotechnologie des Champignons Filamenteux, 163 avenue de Luminy, 13288 Marseille cedex 09, France. Electronic address: craig.faulds@univ-amu.fr.
authors.forename: Craig B
authors.initials: CB
authors.lastname: Faulds
mesh_terms: D019342:Acetic Acid / Q000032:analysis; D018533:Biomass; D002241:Carbohydrates / Q000032:analysis; D002265:Carboxylic Ester Hydrolases; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D031782:Pennisetum / Q000187:drug effects / Q000378:metabolism*; D024301:Plant Bark / Q000187:drug effects / Q000378:metabolism*; D018547:Plant Stems / Q000187:drug effects / Q000378:metabolism*; D012995:Solubility; D013997:Time Factors
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D008031:Lignin; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D019342:Acetic Acid; D004121:Dimethyl Sulfoxide
keywords: Acetyl esterase; Biomass utilisation; Co-solvents; Energy crop; Glycoside hydrolases