title: An inter-machine comparison of tobacco smoke particle deposition in vitro from six independent smoke exposure systems.
abstract: There are several whole smoke exposure systems used to assess the biological and toxicological impact of tobacco smoke in vitro. One such system is the Vitrocell® VC 10 Smoking Robot and exposure module. Using quartz crystal microbalances (QCMs) installed into the module, we were able to assess tobacco smoke particle deposition in real-time. We compared regional deposition across the module positions and doses delivered by six VC 10s in four independent laboratories: two in the UK, one in Germany and one in China. Gauge R&r analysis was applied to the total data package from the six VC 10s. As a percentage of the total, reproducibility (between all six VC 10s) and repeatability (error within an individual VC 10) accounted for 0.3% and 7.4% respectively. Thus Gauge R&r was 7.7%, less than 10% overall and considered statistically fit for purpose. The dose-responses obtained from the six machines across the four different locations demonstrated excellent agreement. There were little to no positional differences across the module at all airflows as determined by ANOVA (except for one machine and at three airflows only). These results support the on-going characterisation of the VC 10 exposure system and suitability for tobacco smoke exposure in vitro.
authors.affiliation: British American Tobacco, Group R&D, Southampton SO15 8TL, UK. Electronic address: jason_adamson@bat.com.
authors.forename: J
authors.initials: J
authors.lastname: Adamson
authors.affiliation: British American Tobacco, Group R&D, Southampton SO15 8TL, UK. Electronic address: david_thorne@bat.com.
authors.forename: D
authors.initials: D
authors.lastname: Thorne
authors.affiliation: British American Tobacco, Group R&D, Southampton SO15 8TL, UK. Electronic address: graham_errington@bat.com.
authors.forename: G
authors.initials: G
authors.lastname: Errington
authors.affiliation: R.J. Reynolds Tobacco Co., P.O. Box 1487, Winston-Salem, NC 27102, USA. Electronic address: FIELDSW@RJRT.com.
authors.forename: W
authors.initials: W
authors.lastname: Fields
authors.affiliation: Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, No.2 Fengyang Street, High-Tech Zone, Zhengzhou, PR China. Electronic address: lixiang79ben@hotmail.com.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.affiliation: Covance Laboratories Ltd., Otley Road, Harrogate HG3 1PY, UK. Electronic address: Rebecca.payne@covance.com.
authors.forename: R
authors.initials: R
authors.lastname: Payne
authors.affiliation: Vitrocell® Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany. Electronic address: t.krebs@vitrocell.com.
authors.forename: T
authors.initials: T
authors.lastname: Krebs
authors.affiliation: British American Tobacco, Group R&D, Southampton SO15 8TL, UK. Electronic address: annette_dalrymple@bat.com.
authors.forename: A
authors.initials: A
authors.lastname: Dalrymple
authors.affiliation: R.J. Reynolds Tobacco Co., P.O. Box 1487, Winston-Salem, NC 27102, USA. Electronic address: FOWLERK2@RJRT.com.
authors.forename: K
authors.initials: K
authors.lastname: Fowler
authors.affiliation: British American Tobacco, Group R&D, Southampton SO15 8TL, UK. Electronic address: debbie_dillon@bat.com.
authors.forename: D
authors.initials: D
authors.lastname: Dillon
authors.affiliation: Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, No.2 Fengyang Street, High-Tech Zone, Zhengzhou, PR China. Electronic address: xiefuwei@sina.com.
authors.forename: F
authors.initials: F
authors.lastname: Xie
authors.affiliation: British American Tobacco, Group R&D, Southampton SO15 8TL, UK. Electronic address: clive_meredith@bat.com.
authors.forename: C
authors.initials: C
authors.lastname: Meredith
mesh_terms: D000280:Administration, Inhalation; D057205:Automation, Laboratory / Q000295:instrumentation*; D015203:Reproducibility of Results; D012371:Robotics; D012906:Smoke; D018675:Toxicity Tests / Q000295:instrumentation*; D014026:Nicotiana
chemical_list: D012906:Smoke
keywords: Dosimetry; In vitro exposure system; Particle deposition; Quartz crystal microbalance; Tobacco smoke; Vitrocell