title: Hydrophilic organic/salt-containing aqueous two-phase solvent system for counter-current chromatography: a novel technique for separation of polar compounds.
abstract: Hydrophilic organic/salt-containing aqueous two-phase system composing of ethanol, water and ammonium sulfate for separation polar compounds was investigated on multilayer coil associated with J-type HSCCC devices. Compared to the classical polar solvent system based on 1-butanol-water or PEG1000-ammonium sulfate-water, the water content of upper phase in ethanol-ammonium sulfate-water systems was from 53.7% to 32.8% (wt%), closed to PEG1000-ammonium sulfate-water aqueous two-phase systems and higher than 1-butanol-water (22.0%, wt%). Therefore, the polarity of ethanol-ammonium sulfate-water is in the middle of 1-butanol-water and PEG-ammonium sulfate-water system, which is quite good for separating polar compounds like phenols, nucleosides and amino acids with low partition coefficient in 1-octanol-water system. The retention of stationary phase in four elution mode on type-J counter-current chromatography devices with multilayer coil column changed from 26% to 71%. Hydrodynamic trend possess both intermediate and hydrophilic solvent system property, which closely related to the composition of solvent system. The applicability of this system was demonstrated by successful separation of adenosine, uridine guanosine and cytidine.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zou
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Gao
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gu
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China; Institute of Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing, China. Electronic address: hbxiao@dicp.ac.cn.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Xiao
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D020003:1-Octanol / Q000737:chemistry; D000241:Adenosine / Q000302:isolation & purification; D000645:Ammonium Sulfate / Q000737:chemistry; D003377:Countercurrent Distribution; D003562:Cytidine / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D006151:Guanosine / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D011092:Polyethylene Glycols / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D014529:Uridine / Q000302:isolation & purification; D014867:Water / Q000737:chemistry
chemical_list: D012492:Salts; D012997:Solvents; D014867:Water; D006151:Guanosine; D000431:Ethanol; D011092:Polyethylene Glycols; D003562:Cytidine; D020001:1-Butanol; D000241:Adenosine; D020003:1-Octanol; D000645:Ammonium Sulfate; D014529:Uridine
keywords: Elution mode; High-speed counter-current chromatography; Hydrophilic organic/salt-containing aqueous two-phase system; Partition coefficient