title: In vitro evaluation of the antioxidant and cytotoxic activities of constituents of the mangrove Lumnitzera racemosa Willd.
abstract: This study performed phytochemical and bioactive assessments of the mangrove Lumnitzera racemosa Willd. leaves. Bioassay-guided fractionation of the methanolic extracts led to the identification of thirty-six compounds (1-36), their structures were elucidated using detailed NMR spectroscopic and MS analysis. The extracts, fractions, and the isolated compounds were screened for potential antioxidant and cytotoxic activities. Antioxidant assays were performed using peroxyl radical-scavenging and reducing assays, whereas cytotoxicity was measured using MTT assays in HL-60 and Hel-299 cell lines. The methanolic extract, CH2Cl2 and n-BuOH fractions (10.0 μg/mL) exhibited potent antioxidant activity, with Trolox equivalent (TE) values of 24.94 ± 0.59, 28.34 ± 0.20, and 27.09 ± 0.37 (μM), respectively. In addition, the isolated compounds exerted cytotoxic effects in a dose-dependent manner; compounds 1 and 14 exhibited the most potent cytotoxicity in HL-60 cells, with IC50 values of 0.15 ± 0.29 and 0.60 ± 0.16 μM, respectively. To clarify the mechanism(s) behind these cytotoxic effects, we measured the time-dependent changes in apoptotic markers including the condensation and fragmentation of nuclear chromatin, and the downregulation of p-ERK1/2, p-AKT, and c-Myc levels.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: Phuong Thao
authors.initials: PT
authors.lastname: Nguyen
authors.forename: Thi Thuy Luyen
authors.initials: TT
authors.lastname: Bui
authors.forename: Ngoc Diep
authors.initials: ND
authors.lastname: Chau
authors.forename: Huu Tai
authors.initials: HT
authors.lastname: Bui
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Kim
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Kang
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Hae Dong
authors.initials: HD
authors.lastname: Jang
authors.forename: The Cuong
authors.initials: TC
authors.lastname: Nguyen
authors.forename: Van Thanh
authors.initials: VT
authors.lastname: Nguyen
authors.forename: Xuan Cuong
authors.initials: XC
authors.lastname: Nguyen
authors.forename: Hoai Nam
authors.initials: HN
authors.lastname: Nguyen
authors.forename: Van Minh
authors.initials: VM
authors.lastname: Chau
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D029764:Combretaceae; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D018922:HL-60 Cells; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves
chemical_list: D000975:Antioxidants; D003603:Cytotoxins; D010936:Plant Extracts