title: Preparation, characterization, in vitro bioactivity, and cellular responses to a polyetheretherketone bioactive composite containing nanocalcium silicate for bone repair.
abstract: In this study, a nanocalcium silicate (n-CS)/polyetheretherketone (PEEK) bioactive composite was prepared using a process of compounding and injection-molding. The mechanical properties, hydrophilicity, and in vitro bioactivity of the composite, as well as the cellular responses of MC3T3-E1 cells (attachment, proliferation, spreading, and differentiation) to the composite, were investigated. The results showed that the mechanical properties and hydrophilicity of the composites were significantly improved by the addition of n-CS to PEEK. In addition, an apatite-layer formed on the composite surface after immersion in simulated body fluid (SBF) for 7 days. In cell culture tests, the results revealed that the n-CS/PEEK composite significantly promoted cell attachment, proliferation, and spreading compared with PEEK or ultrahigh molecular weight polyethylene (UHMWPE). Moreover, cells grown on the composite exhibited higher alkaline phosphatase (ALP) activity, more calcium nodule-formation, and higher expression levels of osteogenic differentiation-related genes than cells grown on PEEK or UHMWPE. These results indicated that the incorporation of n-CS to PEEK could greatly improve the bioactivity and biocompatibility of the composite. Thus, the n-CS/PEEK composite may be a promising bone repair material for use in orthopedic clinics.
authors.affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai 200011, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.forename: Songchao
authors.initials: S
authors.lastname: Tang
authors.forename: Honglue
authors.initials: H
authors.lastname: Tan
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Wentao
authors.initials: W
authors.lastname: Lin
authors.forename: Yugang
authors.initials: Y
authors.lastname: Wang
authors.forename: Changsheng
authors.initials: C
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.forename: Tingting
authors.initials: T
authors.lastname: Tang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry; D001031:Apatites / Q000737:chemistry; D001577:Benzophenones; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration / Q000187:drug effects*; D017610:Calcium Compounds / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D007659:Ketones / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D010012:Osteogenesis; D020959:Polyethylene / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers; D017640:Silicates / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D014961:X-Ray Diffraction
chemical_list: D000880:Anthraquinones; D001031:Apatites; D001577:Benzophenones; D001672:Biocompatible Materials; D017610:Calcium Compounds; D007659:Ketones; D011108:Polymers; D017640:Silicates; C063834:polyetheretherketone; D011092:Polyethylene Glycols; C010078:alizarin; D020959:Polyethylene; C031293:calcium silicate; D012825:Silicon