title: Influence of interleukin 10 promoter polymorphisms in polymyalgia rheumatica: disease susceptibility and functional consequences.
abstract: OBJECTIVE
  To investigate the functional consequences of IL10 (-592C/A and -1082A/G) gene polymorphisms and their association with susceptibility to, and disease phenotype, in patients with polymyalgia rheumatica (PMR).
  
  
  METHODS
  A total number of 168 with PMR and 124 age-matched controls were genotyped using allele-specific primers and restriction fragment length polymorphism analysis. The levels of circulating IL10 and the production of IL10 by PBMCs after in vitro stimulation were studied by Cytometric Bead Array.
  
  
  RESULTS
  No significant differences were observed in genotype or allele frequency distribution between patients and controls. The clinical characteristics and prognosis of these patients were also unrelated to the presence of these polymorphisms. No significant differences between PMR patients with low ESR (<40 mm/hr) and classic PMR (>40 mm/hr) were found. Furthermore, we did not observe any influence of circulating IL10 with the intensity of the acute phase response. In both, PMR patients and age-matched controls, no differences in circulating IL10 levels or IL10 production were observed depending on the genotypes of the IL10 gene.
  
  
  CONCLUSIONS
  These results do not support the impact of IL10 variants in susceptibility or clinical phenotype of PMR patients. In this aged population no functional association was found between IL10 gene variants and IL10 production.
authors.affiliation: Rheumatology Department, Hospital Universitario Marqués de Valdecilla-IFIMAV, Santander, Spain. lorenalvar@hotmail.com.
authors.forename: Lorena
authors.initials: L
authors.lastname: Alvarez-Rodriguez
authors.forename: Eugenio
authors.initials: E
authors.lastname: Carrasco-Marín
authors.forename: Gaurav
authors.initials: G
authors.lastname: Tripathi
authors.forename: Pedro
authors.initials: P
authors.lastname: Muñoz-Cacho
authors.forename: Marcos
authors.initials: M
authors.lastname: Lopez-Hoyos
authors.forename: Cristina
authors.initials: C
authors.lastname: Mata
authors.forename: Jaime
authors.initials: J
authors.lastname: Calvo-Alén
authors.forename: Maite
authors.initials: M
authors.lastname: Garcia-Unzueta
authors.forename: Elena
authors.initials: E
authors.lastname: Aurrecoechea
authors.forename: Carmen
authors.initials: C
authors.lastname: Alvarez-Dominguez
authors.forename: Victor M
authors.initials: VM
authors.lastname: Martinez-Taboada
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001799:Blood Sedimentation; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic; D011111:Polymyalgia Rheumatica / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology*; D011237:Predictive Value of Tests; D011379:Prognosis; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10