title: Double cisterna magna blood injection model of experimental subarachnoid hemorrhage in dogs.
abstract: Several animal subarachnoid hemorrhage (SAH) models have been proposed to study the etiology and treatment for cerebral vasospasm. We describe the experimental procedures of a canine double-hemorrhage model of SAH and discuss the pathophysiological parameters and occurrence of angiographic delayed cerebral vasospasm using magnetic resonance (MR) imaging and digital subtraction angiography. Autologous blood was injected twice on days 1 and 3 into the cerebellomedullary cistern of 36 female beagles. All animals showed delayed angiographic vasospasm in the vertebrobasilar arteries on day 7. The degree of vasospasm was 29-42 % of the arterial diameter. However, this model showed no symptomatic vasospasm or ischemic changes detected by MR imaging. This animal model can produce reproducible delayed vasospasm without detectable cerebral infarction on MR imaging. This model allows evaluation of the effect of treatment on delayed vasospasm in the same animals. The canine double-hemorrhage model of SAH is suitable for the quantitative and chronological study of delayed angiographic vasospasm, but not for investigating early brain injury and delayed cerebral ischemia.
authors.affiliation: Department of Neurosurgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan, kmori@ndmc.ac.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Mori
mesh_terms: D000818:Animals; D001488:Basilar Artery / Q000473:pathology; D001804:Blood Transfusion, Autologous; D002946:Cisterna Magna / Q000601:surgery; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D008279:Magnetic Resonance Imaging; D008297:Male; D013345:Subarachnoid Hemorrhage / Q000150:complications*; D020301:Vasospasm, Intracranial / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*