title: Preparation of polyphosphazene hydrogels for enzyme immobilization.
abstract: We report on the synthesis and application of a new hydrogel based on a methacrylate substituted polyphosphazene. Through ring-opening polymerization and nucleophilic substitution, poly[bis(methacrylate)phosphazene] (PBMAP) was successfully synthesized from hexachlorocyclotriphosphazene. By adding PBMAP to methacrylic acid solution and then treating with UV light, we could obtain a cross-linked polyphosphazene network, which showed an ultra-high absorbency for distilled water. Lipase from Candida rugosa was used as the model lipase for entrapment immobilization in the hydrogel. The influence of methacrylic acid concentration on immobilization efficiency was studied. Results showed that enzyme loading reached a maximum of 24.02 mg/g with an activity retention of 67.25% when the methacrylic acid concentration was 20% (w/w).
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. education@zju.edu.cn.
authors.forename: Yue-Cheng
authors.initials: YC
authors.lastname: Qian
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. chenpengcheng@zju.edu.cn.
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. guikinghe@zju.edu.cn.
authors.forename: Gui-Jin
authors.initials: GJ
authors.lastname: He
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. hxjzxh@zju.edu.cn.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. xuzk@zju.edu.cn.
authors.forename: Zhi-Kang
authors.initials: ZK
authors.lastname: Xu
mesh_terms: D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D020100:Hydrogels / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D004800:Enzymes, Immobilized; D020100:Hydrogels; D008689:Methacrylates; D009943:Organophosphorus Compounds; D011108:Polymers; C108974:poly(phosphazene); C008384:methacrylic acid; D008049:Lipase