title: Comparative Study of Biofilm Formation in Pseudomonas aeruginosa Isolates from Patients of Lower Respiratory Tract Infection.
abstract: BACKGROUND
  This study assessed biofilm formations of P.aeruginosa which was isolated from patients with Lower Respiratory Tract Infections (LRTIs).
  
  
  OBJECTIVE
  This study was conducted to compare different methods of biofilm formations seen in P. aeruginosa which was obtained from LRTI patients.
  
  
  METHODS
  In this cross-sectional study, we investigated a total of 80 P. aeruginosa isolates obtained from LRTI patients by different methods. Tube method (TM), tissue culture plate (TCP) method and modified tissue culture plate (MTCP) method. They were subjected to biofilm detection methods.
  
  
  RESULTS
  The MTCP method produced a higher accuracy ratio than TCP method. In terms of sensitivity and specificity, the MTCP method was considered to be superior to TM. We observed a higher antibiotic resistance in biofilm producing bacteria than in non-biofilm producers.
  
  
  CONCLUSIONS
  In our study, MTCP was found to be more sensitive and specific method for biofilm detection than TCP and TM.
authors.affiliation: Student M.sc, Department of Pulmonary Medicine, King George Medical University , Lucknow, UP, India .
authors.forename: Shivani
authors.initials: S
authors.lastname: Saxena
authors.affiliation: Professor, Department of Microbiology, King George Medical University , Lucknow, UP, India .
authors.forename: Gopa
authors.initials: G
authors.lastname: Banerjee
authors.affiliation: Professor, Department of Pulmonary Medicine, King George Medical University , Lucknow, UP, India .
authors.forename: Rajiv
authors.initials: R
authors.lastname: Garg
authors.affiliation: Professor, Department of Microbiology, King George Medical University , Lucknow, UP, India .
authors.forename: Mastan
authors.initials: M
authors.lastname: Singh
keywords: Biofilm formation; Lower respiratory tract infection; P. aeruginosa; Tissue culture plate