title: Eosinophil chemotaxis.
abstract: Chemotaxis assays have a number of applications in the study of leukocyte biology and immune/inflammatory pathology. Multiwell "blind chamber"-type assays allow a large number of parallel measurements within a single assay. The development of fluorescence assays using microplate-based chemotaxis chambers has permitted a degree of automation to be applied to these assays. Here, a method is described for the quantitative measurement of eosinophil migration using a 96-well assay with numbers of cells that may realistically be obtained from blood samples.
authors.affiliation: Institute of Science & Technology in Medicine, Keele University, Keele, Staffordshire, ST5 5BG, UK, g.dent@keele.ac.uk.
authors.forename: Gordon
authors.initials: G
authors.lastname: Dent
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D004804:Eosinophils / Q000166:cytology* / Q000502:physiology*; D006801:Humans