title: Thermosipho activus sp. nov., a thermophilic, anaerobic, hydrolytic bacterium isolated from a deep-sea sample.
abstract: A novel obligately anaerobic, extremely thermophilic, organotrophic bacterium, strain Rift-s3(T), was isolated from a deep-sea sample containing Riftia pachyptila sheath from Guaymas Basin, Gulf of California. Cells of the novel isolate were rods, 0.3-0.8 µm in width and 1.5-10 µm in length, surrounded by a sheath-like structure (toga). Strain Rift-s3(T) grew at temperatures ranging from 44 to 75 °C, at pH 5.5 to 8.0, and with NaCl concentrations of 3 to 60 g l(-1). Under optimum conditions (65 °C, pH 6.0, NaCl 25 g l(-1)), the doubling time was 30 min. The isolate was able to ferment mono-, oligo- and polysaccharides including cellulose, chitin, xylan and pectin, and proteins including β-keratins, casein and gelatin. Acetate, hydrogen and carbon dioxide were the main products of glucose fermentation. The G+C content of the DNA was 30 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed the affiliation of strain Rift-s3(T) with the genus Thermosipho, with Thermosipho atlanticus Ob7(T) as the closest relative (96.5 % 16S rRNA gene sequence similarity). Based on the phylogenetic analysis and physiological properties of the novel isolate we propose a novel species of the genus Thermosipho, Thermosipho activus sp. nov., with Rift-s3(T) ( = DSM 26467(T) = VKM B-2803(T)) as the type strain.
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Podosokorskaya
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia.
authors.forename: Elizaveta A
authors.initials: EA
authors.lastname: Bonch-Osmolovskaya
authors.affiliation: Ifremer, CNRS, University of Brest, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Unité Etude des Ecosystèmes Profonds, 29280 Plouzané, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Godfroy
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia.
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Gavrilov
authors.affiliation: Gubkin Russian State University of Oil and Gas, Leninskiy Prospect 65, 119991 Moscow, Russia.
authors.forename: Daria A
authors.initials: DA
authors.lastname: Beskorovaynaya
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia.
authors.forename: Tatyana G
authors.initials: TG
authors.lastname: Sokolova
authors.affiliation: Bioengineering Center, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/1, 117312 Moscow, Russia.
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Kolganova
authors.affiliation: Immanuel Kant Baltic Federal University, Botkina str. 3, 236016, Kaliningrad, Russia.
authors.forename: Stepan V
authors.initials: SV
authors.lastname: Toshchakov
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya 7/2, 117312 Moscow, Russia.
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Kublanov
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002140:California; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005285:Fermentation; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S