title: Synthesis of zinc oxide nanoparticles on graphene-carbon nanotube hybrid for glucose biosensor applications.
abstract: Synthesis of zinc oxide nanoparticles incorporated graphene-carbon nanotubes hybrid (GR-CNT-ZnO) through a simple, one-pot method is demonstrated. The as-synthesized GR-CNT-ZnO composite is applied to fabricate an enzyme based glucose biosensor. The GOx immobilized on GR-CNT-ZnO composite exhibits well-defined redox peaks with a peak potential separation (ΔEp) of about 26 mV with enhanced peak currents, indicating a fast electron transfer at the modified electrode surface. The cyclic voltammetry measurements revealed that the modified film has high electrocatalytic ability towards glucose detection in the presence of oxygen. The proposed sensor has a wide linear detection range from 10 μM to 6.5 mM of glucose with a limit of detection (LOD) of 4.5 (±0.08) μM. In addition, the sensor possessed appreciable repeatability, reproducibility and remarkable stability for the sensitive determination of glucose. The practicality of this sensor has been demonstrated in human serum samples, with results being in good agreement with those determined using a standard photometric method.
authors.affiliation: Department of Molecular Science and Engineering, National Taipei University of Technology, Taipei, Taiwan; Institute of Organic and Polymeric Materials, National Taipei University of Technology, Taipei, Taiwan; Centre for Biomedical Industry, National Taipei University of Technology, Taipei, Taiwan. Electronic address: kyhwa@ntut.edu.tw.
authors.forename: Kuo-Yuan
authors.initials: KY
authors.lastname: Hwa
authors.affiliation: Department of Molecular Science and Engineering, National Taipei University of Technology, Taipei, Taiwan.
authors.forename: Boopathi
authors.initials: B
authors.lastname: Subramani
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006108:Graphite; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000648:ultrastructure; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction; D015034:Zinc Oxide
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose; D015034:Zinc Oxide
keywords: Biosensor; Carbon nanotube; Glucose oxidase; Graphene; Zinc oxide