title: Ultrasensitive electrochemical biosensor based on graphite oxide, Prussian blue, and PTC-NH2 for the detection of α2,6-sialylated glycans in human serum.
abstract: α2,6-Sialylated glycans are crucial molecular targets for cancer diagnosis and clinical research. In this work, a novel ultrasensitive electrochemical biosensor was fabricated based on a graphite oxide (GO), Prussian blue (PB), and PTC-NH2 (an ammonolysis product of 3,4,9,10-perylenetetracarboxylic dianhydride) nanocomposite for the selective detection of α2,6-sialylated glycans. To increase the sensitivity of the electrochemical biosensor, gold nanoparticles (GNPs) were immobilized on a GO-PB-PTC-NH2 modified glassy carbon electrode (GCE). Sambucus nigra agglutinins (SNAs), which specifically bind with α2,6-sialylated glycans, were covalently immobilized on GNPs for the sensitive detection of α2,6-sialylated glycans in serum. This proposed method can be applied to human serum, and it worked well over a broad linear range (0.1 pg mL(-1)-500 ng mL(-1)) with detection limits of 0.03 pg mL(-1). Moreover, recovery of the spiked samples ranged from 100.2% to 105.0%, suggesting that this excellent electrochemical biosensor can be used for the practical detection of α2,6-sialylated glycans.
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Box 174♯, No. 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Liuliu
authors.initials: L
authors.lastname: Gao
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Box 174♯, No. 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Junlin
authors.initials: J
authors.lastname: He
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Box 174♯, No. 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Wailan
authors.initials: W
authors.lastname: Xu
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Box 174♯, No. 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Box 174♯, No. 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Junmin
authors.initials: J
authors.lastname: Hui
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Box 174♯, No. 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Box 174♯, No. 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Life Science and School of Public Health, Chongqing Medical University, Box 174♯, No. 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China. Electronic address: yuchaom@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
mesh_terms: D000812:Anhydrides / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D019158:N-Acetylneuraminic Acid / Q000032:analysis* / Q000097:blood; D010087:Oxides / Q000737:chemistry; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry
chemical_list: C521594:3,4,9,10-perylenetetracarboxylic dianhydride; D000812:Anhydrides; D005295:Ferrocyanides; D010087:Oxides; D011134:Polysaccharides; D010569:Perylene; D006108:Graphite; D019158:N-Acetylneuraminic Acid; C000170:ferric ferrocyanide
keywords: Gold nanoparticles; Graphite oxide; PTC-NH(2); Prussian blue; α2,6-Sialylated glycans