title: [Detection of circulating tumor cells in patients with hepatocellular carcinoma].
abstract: OBJECTIVE
  To explore the detection efficiency of circulating tumor cells (CTCs) in patients with hepatocellular carcinoma (HCC).
  
  
  METHODS
  Immunomagnetic negative enrichment by nanometer magnetic beads and label-free capture with Captor(TM) system were used to isolate and enrich CTCs from peripheral blood of HCC patients, and epithelial and HCC markers were applied to identify CTCs by immunofluorescence staining. CTCs were detected in 50 HCC patients before and after hepatectomy to test the method for isolation, enrichment and identification. The dynamic changes of pre- and post-operative CTCs' numbers were compared. The clinical data were analyzed using SPSS 19.0 software.
  
  
  RESULTS
  Negative enrichment methods by nanometer magnetic beads and label-free capture using Captor(TM) system were both suitable for CTCs isolation and enrichment in HCC patients. The positive detection rate of CTCs in HCC patients via negative enrichment was 96.0% (48/50), the preoperative median number of CTCs was 16 per 7.5 ml blood, and the postoperative median number was 17 per 7.5 ml blood.
  
  
  CONCLUSIONS
  Both negative enrichment and Captor(TM) system are suitable for isolation and enrichment of CTCs in HCC patients. There is a significant difference in the numbers of CTCs before and after operation, and dynamic detection of CTCs will provide helpful prognostic information for HCC patients in clinics.
authors.affiliation: State Key Laboratory of Molecular Oncology and Department of Abdominal Surgery, Cancer Institute/ Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Mu
authors.forename: Kaixuan
authors.initials: K
authors.lastname: Lin
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.forename: Yulin
authors.initials: Y
authors.lastname: Sun
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Cai
authors.affiliation: Email: zhaoxh@cicams.ac.cn.
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D006498:Hepatectomy; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000378:metabolism / Q000473:pathology*
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule