title: Toll-like Receptor 1 743 A>G, 1805 T>G & Toll-like Receptor 6 745 C>T gene polymorphism and tuberculosis: a case control study of north Indian population from Agra (India).
abstract: Infection with Mycobacteriumtuberculosis possibly depends on host genetic factors and is thought to be the major cause of differential susceptibility to the disease. In the present study, 205 pulmonary tuberculosis cases and 127 healthy controls were studied for the association of Toll-like Receptor (TLR) variants (TLR1 variants 743A>G and 1805T>G, and TLR6 variant 745 C>T) in north Indian population. The frequency of heterozygous genotypes (AG) in TB cases (0.47) and HCs (0.61), differed significantly (p value = 0.02). The association of AG genotypes in HCs was adjusted for gender as gender was observed to be a confounder and M-H OR was found to be 0.62 (p = 0.044). On categorizing the cases basing on AFB smear positivity, the heterozygous genotypes (AG) was found to be associated with low bacillary load (scanty and 1+) (P = 0.002). No association was observed for either TLR1 1805 T>G or TLR6 745 C>T polymorphism. Level of serum IL6 was found to be significantly higher among healthy controls with TLR1 GG genotype compared to healthy controls with AA (p = 0.035) and AG (p = 0.005) genotypes. Thus, it may be suggested that the heterozygous condition for TLR1 743 A>G provide resistance from the disease. However, in depth study is required to understand the mechanism for possible protective responses.
authors.affiliation: Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, India.
authors.forename: Ekata
authors.initials: E
authors.lastname: Sinha
authors.affiliation: Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, India.
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Biswas
authors.affiliation: Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, India.
authors.forename: Mayank
authors.initials: M
authors.lastname: Mittal
authors.affiliation: State Tuberculosis Demonstration Centre (STDC), Agra, India.
authors.forename: Bharat
authors.initials: B
authors.lastname: Bajaj
authors.affiliation: Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Singh
authors.affiliation: Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Arela
authors.affiliation: Indian Council of Medical Research (Department of Health Research), Ministry of Health and Family Welfare, New Delhi, India.
authors.forename: Vishwa M
authors.initials: VM
authors.lastname: Katoch
authors.affiliation: Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, India. Electronic address: keshar63@yahoo.com.
authors.forename: Keshar Kunja
authors.initials: KK
authors.lastname: Mohanty
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D058491:Bacterial Load; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006579:Heterozygote; D006801:Humans; D007194:India; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011110:Polymorphism, Genetic; D051194:Toll-Like Receptor 1 / Q000235:genetics* / Q000276:immunology; D051216:Toll-Like Receptor 6 / Q000235:genetics* / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000235:genetics* / Q000276:immunology / Q000473:pathology
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; C495368:TLR6 protein, human; D051194:Toll-Like Receptor 1; D051216:Toll-Like Receptor 6
keywords: Acid fast bacillus; Heterozygous genotype; Interleukin 6; North Indian population; Toll-like Receptors