title: Introduction of genes via sonoporation and electroporation.
abstract: Gene therapy delivery using viral vectors has demonstrated efficient transfection but has safety issues. The need for safer yet effective delivery systems has led to the active development of non-viral techniques. In this chapter, we will discuss two evolving techniques - sonoporation with microbubble contrast agents and electroporation with focus on their basic principle, parameters affecting delivery efficiency, current evolving techniques and future prospects.
authors.affiliation: Imperial College London, Hammersmith Hospital, London, UK.
authors.forename: Christina
authors.initials: C
authors.lastname: Kalli
authors.forename: Wey Chyi
authors.initials: WC
authors.lastname: Teoh
authors.forename: Edward
authors.initials: E
authors.lastname: Leen
mesh_terms: D000818:Animals; D053672:Electrochemotherapy / Q000295:instrumentation / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D014162:Transfection / Q000295:instrumentation / Q000379:methods*; D014463:Ultrasonography / Q000295:instrumentation / Q000379:methods*