title: Anticandidal activity of cell extracts from 13 probiotic Lactobacillus strains and characterisation of lactic acid and a novel fatty acid derivative from one strain.
abstract: This study investigated the anti-Candida activity of methanol extracts from freeze-dried probiotic cells and the isolation of some constituents in the extracts. The MIC values of the probiotic methanol cell extracts against Candida albicans ranged between 1.25 and 5mg/ml after 48 h of incubation. However, Lactococcus latics subsp. lactis strain X and Lactobacillus casei strain B extracts had an MIC of 10mg/ml after 48 h of incubation. The extracts had fungistatic rather than fungicidal activity. These extracts had a much higher antifungal activity than antifungal compounds isolated from the growth medium by many other authors. This indicates that probiotics may also release antifungal compounds in their cells that could contribute to a therapeutic effect. Lactic acid (1) and 6-O-(α-D-glucopyranosyl)-1,6-di-O-pentadecanoyl-α-D-glucopyranose a novel fatty acid derivative (2) were isolated from methanol probiotic extracts and the structure of these compounds were elucidated using NMR (1 and 2D) and mass spectrometry (MS).
authors.affiliation: Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa; Tshwane University of Technology, Department of Biotechnology and Food Technology, Building 4-Room 104, Arcadia Campus, Pretoria, South Africa.
authors.forename: Richard
authors.initials: R
authors.lastname: Nyanzi
authors.affiliation: Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa.
authors.forename: Maurice D
authors.initials: MD
authors.lastname: Awouafack
authors.affiliation: Council for Scientific and Industrial Research, Biosciences, P.O. Box 395, Pretoria 001, South Africa.
authors.forename: Paul
authors.initials: P
authors.lastname: Steenkamp
authors.affiliation: Tshwane University of Technology, Department of Biotechnology and Food Technology, Building 4-Room 104, Arcadia Campus, Pretoria, South Africa.
authors.forename: Piet J
authors.initials: PJ
authors.lastname: Jooste
authors.affiliation: Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. Electronic address: kobus.eloff@up.ac.za.
authors.forename: Jacobus N
authors.initials: JN
authors.lastname: Eloff
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D002457:Cell Extracts / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis*; D019344:Lactic Acid / Q000032:analysis*; D007778:Lactobacillus / Q000737:chemistry*; D007780:Lacticaseibacillus casei / Q000254:growth & development; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D019936:Probiotics / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D002457:Cell Extracts; D005227:Fatty Acids; D019344:Lactic Acid
keywords: Anti-Candida; Constituents; Lactobacillus; Probiotic