title: Evaluation of a biomimetic poly(ε-caprolactone)/β-tricalcium phosphate multispiral scaffold for bone tissue engineering: in vitro and in vivo studies.
abstract: In this study, the osteogenic potential of rat bone marrow mesenchymal stem cells (rBM-MSCs) on a biomimetic poly(ε-caprolactone)/β-tricalcium phosphate (PCL/β-TCP) composite scaffold composed of parallel concentric fibrous membranes was evaluated in vitro and in vivo. PCL/β-TCP composite membranes were prepared by electrospinning and characterized by x-ray diffraction, differential scanning calorimetry, Fourier transform-infrared spectroscopy, and scanning electron microscopy (SEM). rBM-MSCs were seeded on three-dimensional multispiral scaffolds prepared by the assembly of composite membranes. The cell-scaffold constructs were cultured in osteogenic medium for 4 weeks. Histochemical studies and biochemical assays confirmed the osteogenic differentiation of rBM-MSCs inside the scaffold by documenting the dense mineralized extracellular matrix formation starting from the second week of culture. In the in vivo part of the study, cell-scaffold constructs precultured for 7 days were implanted subcutaneously into the epigastric groin fascia of Wistar rats for a duration of 6 months. Ectopic bone-tissue like formation was documented by using computerized tomography, confocal laser microscopy, SEM, and histochemistry. In vivo findings indicated that the biomimetic multispiral scaffold seeded with rBM-MSCs supports the ectopic formation of new bone tissue in Wistar rats.
authors.affiliation: Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science and Ankara University Stem Cell Institute, Ankara 06100, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Baykan
authors.affiliation: Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science and Ankara University Stem Cell Institute, Ankara 06100, Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Koc
authors.affiliation: Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science and Ankara University Stem Cell Institute, Ankara 06100, Turkey.
authors.forename: Ayse
authors.initials: A
authors.lastname: Eser Elcin
authors.affiliation: Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University Faculty of Science and Ankara University Stem Cell Institute, Ankara 06100, Turkey.
authors.forename: Yasar
authors.initials: Y
authors.lastname: Murat Elcin
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry* / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000473:pathology; D002130:Calcium Phosphates / Q000737:chemistry*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010012:Osteogenesis / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering; D014057:Tomography, X-Ray Computed
chemical_list: D002130:Calcium Phosphates; D011091:Polyesters; C485817:beta-tricalcium phosphate; C016240:polycaprolactone