title: The effect of temperature on the extrinsic incubation period and infection rate of dengue virus serotype 2 infection in Aedes albopictus.
abstract: Dengue fever is an acute mosquito-borne viral disease caused by dengue virus (DENV). Temperature may affect the efficiency of the mosquito vectors in spreading DENV. Aedes albopictus mosquitoes were infected orally with a DENV2 suspension and incubated at different temperatures. Subsequently, DENV2 antigen was collected from salivary gland and thorax-abdomen samples on different days postinfection and tested using an immunofluorescence assay to determine the extrinsic incubation period and infection rate. As the temperature increased, the extrinsic DENV2 incubation period in Ae. albopictus gradually shortened, and infection rates showed a tendency to initially increase, followed by a subsequent decrease.
authors.affiliation: Fujian Center for Disease Control and Prevention, Fuzhou, 350001, People's Republic of China.
authors.forename: Fang-Zhen
authors.initials: FZ
authors.lastname: Xiao
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Deng
authors.forename: Si
authors.initials: S
authors.lastname: He
authors.forename: Han-Guo
authors.initials: HG
authors.lastname: Xie
authors.forename: Xiao-Nong
authors.initials: XN
authors.lastname: Zhou
authors.forename: Yan-Sheng
authors.initials: YS
authors.lastname: Yan
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D003716:Dengue Virus / Q000145:classification / Q000502:physiology*; D005260:Female; D007303:Insect Vectors / Q000821:virology*; D008297:Male; D012469:Salivary Glands / Q000821:virology; D013696:Temperature