title: p120 modulates LPS-induced NF-κB activation partially through RhoA in bronchial epithelial cells.
abstract: p120-Catenin (p120) is an adherens junction protein recognized to regulate cell-cell adhesion. Emerging evidence indicates that p120 may also play an important role in inflammatory responses, and the regulatory mechanisms are still unknown. In the present study, we showed that p120 was associated with airway inflammation. p120 downregulation induced nuclear factor-κB (NF-κB) activation, accompanied with I κ B α degradation, p65 nuclear translocation, and increased expression of interleukin-8 (IL-8) in lipopolysaccharide (LPS)- treated C57BL mice and human bronchial epithelial cells (BECs). Moreover, we first found that p120 directly coprecipitated with RhoA in BECs. After LPS stimulation, although total RhoA and p120-bound RhoA were unchanged, RhoA activity was increased. Y27632, a ROCK inhibitor, could partially inhibit nuclear translocation of p65. Overexpression of p120 inactivated RhoA and NF-κB in BECs, whereas p120 loss significantly increased RhoA activity, p65 nuclear translocation, and IL-8 expression. Taken together, our study supports the regulatory role of p120 in airway inflammation and reveals that p120 may modulate NF-κB signaling partially through RhoA.
authors.affiliation: Key Laboratory of Pulmonary Disease of Ministry of Health of China, Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Lingzhi
authors.identifier: 0000-0003-4958-5241
authors.initials: L
authors.lastname: Qin
authors.affiliation: Key Laboratory of Pulmonary Disease of Ministry of Health of China, Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Shenghui
authors.initials: S
authors.lastname: Qin
authors.affiliation: Key Laboratory of Pulmonary Disease of Ministry of Health of China, Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Pulmonary Disease of Ministry of Health of China, Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Chao
authors.identifier: 0000-0002-1458-8170
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Pulmonary Disease of Ministry of Health of China, Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Heng
authors.identifier: 0000-0002-1141-7405
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Pulmonary Disease of Ministry of Health of China, Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Naping
authors.identifier: 0000-0001-5398-7986
authors.initials: N
authors.lastname: Li
authors.affiliation: Key Laboratory of Pulmonary Disease of Ministry of Health of China, Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pulmonary Disease of Ministry of Health of China, Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Xi
authors.identifier: 0000-0003-4378-3431
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Pulmonary Disease of Ministry of Health of China, Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Renliang
authors.initials: R
authors.lastname: Wu
mesh_terms: D000818:Animals; D001980:Bronchi / Q000378:metabolism / Q000473:pathology; D051177:Catenins / Q000235:genetics* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D016209:Interleukin-8 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000633:toxicity; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D020742:rhoA GTP-Binding Protein / Q000096:biosynthesis* / Q000235:genetics; D000095002:Delta Catenin
chemical_list: D051177:Catenins; D016209:Interleukin-8; D008070:Lipopolysaccharides; D016328:NF-kappa B; C066737:RHOA protein, human; D020742:rhoA GTP-Binding Protein; D000095002:Delta Catenin; C000722347:CTNND1 protein, human