title: Stepwise splitting of ribosomal proteins from yeast ribosomes by LiCl.
abstract: Structural studies have revealed that the core of the ribosome structure is conserved among ribosomes of all kingdoms. Kingdom-specific ribosomal proteins (r-proteins) are located in peripheral parts of the ribosome. In this work, the interactions between rRNA and r-proteins of eukaryote Saccharomyces cerevisiae ribosome were investigated applying LiCl induced splitting and quantitative mass spectrometry. R-proteins were divided into four groups according to their binding properties to the rRNA. Most yeast r-proteins are removed from rRNA by 0.5-1 M LiCl. Eukaryote-specific r-proteins are among the first to dissociate. The majority of the strong binders are known to be required for the early ribosome assembly events. As compared to the bacterial ribosome, yeast r-proteins are dissociated from rRNA at lower ionic strength. Our results demonstrate that the nature of protein-RNA interactions in the ribosome is not conserved between different kingdoms.
authors.affiliation: Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
authors.forename: Kerli
authors.initials: K
authors.lastname: Piir
authors.affiliation: Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
authors.forename: Tiina
authors.initials: T
authors.lastname: Tamm
authors.affiliation: Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Kisly
authors.affiliation: Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
authors.forename: Triin
authors.initials: T
authors.lastname: Tammsalu
authors.affiliation: Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
authors.forename: Jaanus
authors.initials: J
authors.lastname: Remme
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007553:Isotope Labeling; D018021:Lithium Chloride / Q000737:chemistry / Q000633:toxicity*; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D020836:Protein Structure, Quaternary; D012335:RNA, Ribosomal / Q000737:chemistry / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D018021:Lithium Chloride