title: 4-Phosphothiophen-2-yl alanine: a new 5-membered analogue of phosphotyrosine.
abstract: Polyclonal antibodies raised against 4-phosphothiophen-2-yl alanine 2a, a novel five-membered ring analogue of phosphotyrosine, showed high selectivity for phosphotyrosine and no cross-reactivity with other phosphorylated amino acids. Western blots showed that the polyclonal was similarly effective, but different in selectivity, to a commercially available monoclonal antibody.
authors.affiliation: Department of Chemistry, The University of Sheffield, Dainton Building, Sheffield, S3 7HF, UK. r.f.w.jackson@shef.ac.uk.
authors.forename: Matthew
authors.initials: M
authors.lastname: Lilley
authors.forename: Bezaleel
authors.initials: B
authors.lastname: Mambwe
authors.forename: Richard F W
authors.initials: RF
authors.lastname: Jackson
authors.forename: Richmond
authors.initials: R
authors.lastname: Muimo
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D015153:Blotting, Western; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000737:chemistry*
chemical_list: D019000:Phosphotyrosine; D000409:Alanine