title: Evaluation of correlation of cell cycle proteins and Ki-67 interaction in paranasal sinus inverted papilloma prognosis and squamous cell carcinoma transformation.
abstract: The recurrent sinonasal inverted papilloma (IP) could be transformed to sinonasal squamous cell carcinoma. We use protein expression patterns by immunohistochemical method to see whether the expression of p53, p16, p21, and p27 belongs to cell-cycle-regulators and PCNA (proliferating cell nuclear antigen) and Ki-67 the proliferation markers in sixty patients with sinonasal inverted papilloma, and 10 of them with squamous cell carcinoma transformation. Significantly elevated levels of Ki-67, p27, and PCNA in IP with squamous cell carcinoma transformation of sinonasal tract compared with inverted papilloma were revealed. No variation of p16, p21, PLUNC (palate, lung, and nasal epithelium clone protein) and p53 expression was correlated to sinonasal IP malignant transformation by multivariate survey. However, we found elevated PLUNC expression in IPs with multiple recurrences. Finally, we found that PCNA, p27 may interact with CDK1 which promote IP cell proliferation and correlate to sinonasal squamous cell carcinoma. Ki-67 could work throughout the cell cycles to cause malignant transformation. In conclusion, this is a first study showing the correlation of Ki-67, PCNA interacted with CDK1 might lead to malignant transformation. Elevated PLUNC expression in the sinonasal IPs was related to multiple recurrences in human.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, China Medical University, Taichung 40402, Taiwan ; Department of Medicine, School of Medicine, China Medical University, Taichung 40402, Taiwan ; Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Yung-An
authors.initials: YA
authors.lastname: Tsou
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 40402, Taiwan.
authors.forename: Hung-Jin
authors.initials: HJ
authors.lastname: Huang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, China Medical University, Taichung 40402, Taiwan.
authors.forename: Tang-Chuan
authors.initials: TC
authors.lastname: Wang
authors.affiliation: Department of Medicine, School of Medicine, China Medical University, Taichung 40402, Taiwan.
authors.forename: Chih-Jaan
authors.initials: CJ
authors.lastname: Tai
authors.affiliation: Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Chuan-Mu
authors.identifier: 0000-0003-2461-9150
authors.initials: CM
authors.lastname: Chen
authors.affiliation: Department of Medicine, School of Medicine, China Medical University, Taichung 40402, Taiwan ; Department of Biomedical Informatics, Asia University, Taichung 41354, Taiwan ; Computational and Systems Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA ; Research Center for Chinese Medicine and Acupuncture, China Medical University, Taichung 40402, Taiwan.
authors.forename: Calvin Yu-Chian
authors.identifier: 0000-0003-3306-250X
authors.initials: CY
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D016203:CDC2 Protein Kinase; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000473:pathology; D018308:Papilloma, Inverted / Q000235:genetics / Q000473:pathology; D010255:Paranasal Sinus Neoplasms / Q000235:genetics* / Q000473:pathology; D010750:Phosphoproteins / Q000096:biosynthesis*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
chemical_list: C485599:BPIFA1 protein, human; D006023:Glycoproteins; D019394:Ki-67 Antigen; D010750:Phosphoproteins; D018809:Proliferating Cell Nuclear Antigen; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C413374:p27 antigen; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases