title: nc886, a non-coding RNA of anti-proliferative role, is suppressed by CpG DNA methylation in human gastric cancer.
abstract: nc886 is a 101 nucleotide long non-coding RNA that has been designated as a precursor microRNA or a vault RNA based upon it sequence. nc886 has also been suggested to be a tumor suppressor, mainly inferred by its expression pattern as well as its genomic location at human chromosome 5q31, a locus for a tumor suppressor gene(s). However, legitimate data based on nc886's correct identity for its functional cellular roles as a tumor suppressor have not been provided yet. Here we have investigated nc886 in gastric cancer where its expression is suppressed due to CpG DNA hypermethylation at its promoter region in a cohort of paired tumor/normal tissues from 88 gastric cancer patients. CpG hypermethylation of nc886 and thus its diminished expression is significantly associated with poor survival in these cancer patients. nc886 inhibits cell proliferation when ectopically expressed in gastric cancer cells. nc886's tumor suppressive role is corroborated by the induction of well-known oncogenes such as FOS, NF-κB, and MYC upon its knockdown. All these activities of nc886 are undoubtedly independent of mature microRNA or vault RNA. Our data indicate that nc886 is a putative tumor suppressor and could potentially be used as a diagnostic marker in gastric cancer.
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX77555-1072, USA; Department of Life Science and Center for Aging and Health Care, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Kwang-Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Jong-Lyul
authors.initials: JL
authors.lastname: Park
authors.forename: Kwanbok
authors.initials: K
authors.lastname: Lee
authors.forename: Lauren E
authors.initials: LE
authors.lastname: Richardson
authors.forename: Betty H
authors.initials: BH
authors.lastname: Johnson
authors.forename: Hyun-Sung
authors.initials: HS
authors.lastname: Lee
authors.forename: Ju-Seog
authors.initials: JS
authors.lastname: Lee
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Kim
authors.forename: Oh-Hyung
authors.initials: OH
authors.lastname: Kwon
authors.forename: Kyu Sang
authors.initials: KS
authors.lastname: Song
authors.forename: Yong Sung
authors.initials: YS
authors.lastname: Kim
authors.forename: Hassan
authors.initials: H
authors.lastname: Ashktorab
authors.forename: Duane T
authors.initials: DT
authors.lastname: Smoot
authors.affiliation: Department of Life Science and Center for Aging and Health Care, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Jeon
authors.affiliation: Medical Genomics Research Center, KRIBB, Daejeon 305-806, Korea;Department of Functional Genomics, University of Science and Technology, Daejeon, 305-806, Korea.
authors.forename: Seon-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX77555-1072, USA.
authors.forename: Yong Sun
authors.initials: YS
authors.lastname: Lee
mesh_terms: D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D018899:CpG Islands; D019175:DNA Methylation; D006801:Humans; D022661:RNA, Untranslated / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection
chemical_list: D022661:RNA, Untranslated