title: Evaluating plant immunity using mass spectrometry-based metabolomics workflows.
abstract: Metabolic processes in plants are key components of physiological and biochemical disease resistance. Metabolomics, the analysis of a broad range of small molecule compounds in a biological system, has been used to provide a systems-wide overview of plant metabolism associated with defense responses. Plant immunity has been examined using multiple metabolomics workflows that vary in methods of detection, annotation, and interpretation, and the choice of workflow can significantly impact the conclusions inferred from a metabolomics investigation. The broad range of metabolites involved in plant defense often requires multiple chemical detection platforms and implementation of a non-targeted approach. A review of the current literature reveals a wide range of workflows that are currently used in plant metabolomics, and new methods for analyzing and reporting mass spectrometry (MS) data can improve the ability to translate investigative findings among different plant-pathogen systems.
authors.affiliation: Proteomics and Metabolomics Facility, Colorado State University Fort Collins, CO, USA ; Department of Soil and Crop Sciences, Colorado State University Fort Collins, CO, USA.
authors.forename: Adam L
authors.initials: AL
authors.lastname: Heuberger
authors.affiliation: Proteomics and Metabolomics Facility, Colorado State University Fort Collins, CO, USA ; Department of Soil and Crop Sciences, Colorado State University Fort Collins, CO, USA.
authors.forename: Faith M
authors.initials: FM
authors.lastname: Robison
authors.affiliation: Proteomics and Metabolomics Facility, Colorado State University Fort Collins, CO, USA.
authors.forename: Sarah Marie A
authors.initials: SM
authors.lastname: Lyons
authors.affiliation: Proteomics and Metabolomics Facility, Colorado State University Fort Collins, CO, USA ; Department of Horticulture and Landscape Architecture, Colorado State University Fort Collins, CO, USA.
authors.forename: Corey D
authors.initials: CD
authors.lastname: Broeckling
authors.affiliation: Proteomics and Metabolomics Facility, Colorado State University Fort Collins, CO, USA ; Department of Biochemistry and Molecular Biology, Colorado State University Fort Collins, CO, USA.
authors.forename: Jessica E
authors.initials: JE
authors.lastname: Prenni
keywords: GC-MS; LC-MS; metabolomics; plant defense; plant pathogen