title: [Potential detrimental effect of soy isoflavones on testis sertoli cells].
abstract: OBJECTIVE
  To determine the effect of soy isoflavones on cell proliferation and the transcription levels of follicle-stimulating hormone receptor (FSHR), inhibin α (INHα), INHβB, androgen binding protein (ABP), transferrin (Tf) and vimentin in testis sertoli cells in SD rats.
  
  
  METHODS
  Sertoli cells were cultured in vitro, exposed to daidzein at 0.03, 0.3, 3, and 30 μmol/L and genistein at 0.05, 0.5, 5 and 50 μmol/L, respectively. MTT was used to detect the proliferation of sertoli cells. Real-time PCR was used to detect the relative mRNA expressions of FSHR, INHα, INHβB, ABP, Tf and vimentin.
  
  
  RESULTS
  Compared with control groups, cell proliferation and the relative mRNA expression levels of INHβB and ABP in the treated cells showed no significant alternation. The INHα mRNA expression levels were increased in 0.3 and 3 μmol/L Dai and 0.05 μmol/L Gen, while the mRNA expression levels of FSHR were downregulated in 30 μmol/L Dai and Gen at all concentrations. Tf mRNA expression levels were downregulated in 30 μmol/L Dai and 5 μmol/L and 50 μmol/L Gen, and the mRNA expression levels of vimentin were downregulated in 3 and 30 μmol/L Dai and 50 μmol/L Gen.
  
  
  CONCLUSIONS
  Soy Isoflavones may have potential detrimental effect on the male reproductive system, as they may impact the function of sertoli cells by downregulating the transcription levels of some important proteins.
authors.affiliation: Department of Nutrition, Food Safety and Toxicology, West China School of Public Health, Sichuan University, Chengdu 610041,China.
authors.forename: Dejiao
authors.initials: D
authors.lastname: Yin
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhu
authors.forename: Linxi
authors.initials: L
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
mesh_terms: D000727:Androgen-Binding Protein / Q000378:metabolism; D000818:Animals; D028322:Inhibin-beta Subunits / Q000378:metabolism; D007265:Inhibins / Q000378:metabolism; D007529:Isoflavones / Q000009:adverse effects*; D008297:Male; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D011962:Receptors, FSH / Q000378:metabolism; D012708:Sertoli Cells / Q000187:drug effects*; D013025:Glycine max / Q000737:chemistry; D013737:Testis / Q000166:cytology / Q000187:drug effects*; D014168:Transferrin / Q000378:metabolism
chemical_list: D000727:Androgen-Binding Protein; D007529:Isoflavones; D012333:RNA, Messenger; D011962:Receptors, FSH; D014168:Transferrin; C094377:inhibin-alpha subunit; D007265:Inhibins; C004742:daidzein; D028322:Inhibin-beta Subunits