title: [Evaluation of the VITEK 2 system (AST-YSO1 cards) for antifungal susceptibility testing against different Candida species].
abstract: The aim of this investigation was to evaluate the results of antifungal susceptibility for various Candida species using the Vitek 2 semi-automated system (AST-YSO1 cards, bioMérieux), and to compare them with those obtained by the CLSI (Clinical and Laboratory Standards Institute) broth microdilution reference method (Document M27-A3,2008). The essential agreement (EA) was > 90%, except for Candida glabrata against voriconazole (VCZ); and for Candida krusei against fluconazole (FCZ). The overall categorical agreement (CA) was > 90% when FCZ was evaluated and 89.5% at 24h and 80.7% at 48 h for VCZ. The average time for obtaining results was 15.5h. Minor errors were 7.8% at 24h and 6.1% at 48 h for FCZ, and 10.5% at 24h and 19.3% at 48 h for VCZ. There was only one very major error for FCZ against Candida parapsilosis and no major errors were observed. For amphotericin B, only three isolates showed MICs ≥ 2 μg/ml. The Vitek 2 system detected the MIC value for various Candida species and showed excellent agreement with the reference method proposed by the CLSI.
authors.affiliation: Sección Microbiología Laboratorio Central, Hospital General de Agudos Dr. C.G. Durand, Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: omarieu@hotmail.com.
authors.forename: María E
authors.initials: ME
authors.lastname: Ochiuzzi
authors.affiliation: Red de Micología CABA, Argentina.
authors.forename: Alicia
authors.initials: A
authors.lastname: Arechavala
authors.affiliation: Red de Micología CABA, Argentina.
authors.forename: Liliana
authors.initials: L
authors.lastname: Guelfand
authors.affiliation: Red de Micología CABA, Argentina.
authors.forename: Ivana
authors.initials: I
authors.lastname: Maldonado
authors.affiliation: Hospital Naval Pedro Mallo, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Rolando
authors.initials: R
authors.lastname: Soloaga
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009172:Mycology / Q000379:methods; D011743:Pyrimidines / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; D000666:Amphotericin B; D015725:Fluconazole; D065819:Voriconazole
keywords: Candida spp; Sensibilidad a los antifúngicos; Susceptibility testing; Vitek 2; Vitek 2 system