title: Influence of casein phosphopeptide-amorphous calcium phosphate application, smear layer removal, and storage time on resin-dentin bonding.
abstract: The aim of this study is to evaluate the influence of Tooth Mousse (TM) application, smear layer removal, and storage time on resin-dentin microtensile bond strength (µTBS). Dentin specimens were divided into two groups: (1) smear layer covered; (2) smear layer removed using 15% EDTA for 90 s. In each group, half the specimens were treated once with TM for 60 min. After bonding procedures using a two-step self-etching adhesive (Clearfil SE Bond (CSE); Kuraray Medical, Tokyo, Japan), an all-in-one adhesive (G-Bond (GB); GC Corp, Tokyo, Japan), and a total-etch adhesive (Adper Single Bond 2 (SB); 3M ESPE, St. Paul, MN, USA), the specimens were stored for 3 d or 6 months in deionized water at 37 °C, and µTBS was tested and analyzed. With the exception of SB (no TM application) and GB, the μTBS was significantly increased for CSE and SB using EDTA pre-conditioning and 3 d of storage (P≤0.001). Bond strength of GB decreased significantly when using EDTA (3 d storage, P<0.05). TM application only increased the μTBS of GB (no EDTA) and SB (with EDTA) after 3 d (P≤0.02). Comparing the adhesives after 3 d of storage, CSE exhibited the greatest μTBS values followed by GB and SB (P≤0.02). The factors of adhesive, EDTA, and TM did not show any significant impact on μTBS when specimens were stored for 6 months (P>0.05). The additional application of TM and EDTA for cavity preparation seems only to have a short-term effect, and no influence on µTBS of dentin bonds after a period of 6 months.
authors.affiliation: Department of Stomatology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; Institute of Medical Informatics and Statistics, University Hospital Schleswig-Holstein, Campus Kiel, 24105 Kiel, Germany; Department of Prosthodontics, Propaedeutics and Dental Materials, Christian-Albrechts University at Kiel, Arnold-Heller-Straße 16, 24105 Kiel, Germany.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.forename: Wei-ying
authors.initials: WY
authors.lastname: Zheng
authors.forename: Peng-ruo-feng
authors.initials: PR
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Hui-ping
authors.initials: HP
authors.lastname: Lin
authors.forename: Yi-jing
authors.initials: YJ
authors.lastname: Fan
authors.forename: Xin-hua
authors.initials: XH
authors.lastname: Gu
authors.forename: Oliver
authors.initials: O
authors.lastname: Vollrath
authors.forename: Christian
authors.initials: C
authors.lastname: Mehl
mesh_terms: D002364:Caseins / Q000737:chemistry*; D001840:Dental Bonding; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012117:Resins, Synthetic; D016369:Smear Layer; D013997:Time Factors
chemical_list: D002364:Caseins; D012117:Resins, Synthetic; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex
keywords: Bond strength; Casein phosphopeptide-amorphous calcium phosphate; Ethylenediaminetetraacetic acid (EDTA); Smear layer; Tooth Mousse