title: Cytotoxic effects of pentachlorophenol (PCP) and its metabolite tetrachlorohydroquinone (TCHQ) on liver cells are modulated by antioxidants.
abstract: The worldwide distribution and high bioaccumulation potential of pentachlorophenol (PCP) in aquatic organisms imply a high toxicological impact in aquatic systems. Firstly, our investigations show that, similar to mammalian cell lines, PCP can be metabolized to tetrachlorohydroquinone (TCHQ) in the permanent cell line derived from rainbow trout liver cells (RTL-W1). Moreover, we demonstrate that PCP as well as its metabolite TCHQ is capable of influencing the viability of these cells. Three cell viability assays were performed to assess possible cellular targets of these substances. Thus, the cytotoxicity of the PCP-derivative TCHQ was shown for the first time in a fish cell line. Further investigations revealed the involvement of ROS in the cytotoxicity of PCP and its metabolite TCHQ. The observation of oxidative stress provides a plausible explanation for the increased cytotoxicity at higher concentrations especially for PCP and implies possible mechanisms underlying these observations. In addition, antioxidants such as ascorbic acid and quercetin modulate the detrimental effects of PCP and TCHQ whereby both compounds exacerbate the cytotoxic effects of high PCP and TCHQ concentrations.
authors.affiliation: Institute of Natural Resource Sciences (IUNR), Zurich University of Applied Sciences (ZHAW), Gruental, P.O. Box, 8820, Waedenswil, Switzerland, constanze.pietsch@zhaw.ch.
authors.forename: Constanze
authors.initials: C
authors.lastname: Pietsch
authors.forename: Juliane
authors.initials: J
authors.lastname: Hollender
authors.forename: Falk
authors.initials: F
authors.lastname: Dorusch
authors.forename: Patricia
authors.initials: P
authors.lastname: Burkhardt-Holm
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D029941:Fish Proteins / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology; D006873:Hydroquinones / Q000633:toxicity*; D020128:Inhibitory Concentration 50; D017686:Oncorhynchus mykiss; D018384:Oxidative Stress; D010416:Pentachlorophenol / Q000633:toxicity*; D011794:Quercetin / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D006873:Hydroquinones; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; C044578:2,3,5,6-tetrachlorohydroquinone; D011794:Quercetin; D010416:Pentachlorophenol; D013482:Superoxide Dismutase; D001205:Ascorbic Acid