title: Bidirectional cytoplasmic incompatibility caused by Wolbachia in the terrestrial isopod Porcellio dilatatus.
abstract: In the terrestrial isopod species Porcellio dilatatus, unidirectional Cytoplasmic Incompatibility (CI) between two morphs (P. d. dilatatus and P. d. petiti) caused by a Wolbachia strain (wPet) infecting the morph P. d. petiti has been previously described by experiments initiated four decades ago. Here, we studied another Wolbachia that has been recently detected in a population of the morph P. d. dilatatus. The MLST markers reveal that this Wolbachia is a new strain called wDil distinct from wPet also belonging to the isopod clade of Wolbachia. Quantifications of both Wolbachia strains in the gonads of the two P. dilatatus morphs revealed that all males exhibit similar Wolbachia titers while the titers in females depend on the Wolbachia strain they host. Crossing experiments showed that both wDil and wPet induced partial unidirectional CI with different intensities. Moreover, these two strains induced bidirectional CI when individuals were both infected with one of the two different Wolbachia strains. This way, we demonstrated that P. dilatatus can be infected by two closely related Wolbachia strains (wDil and wPet), that seem to have different modification-rescue systems.
authors.affiliation: Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Sicard
authors.affiliation: Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Bouchon
authors.affiliation: Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Ceyrac
authors.affiliation: Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France.
authors.forename: Roland
authors.initials: R
authors.lastname: Raimond
authors.affiliation: Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Thierry
authors.affiliation: Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France.
authors.forename: Winka
authors.initials: W
authors.lastname: Le Clec'h
authors.affiliation: Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Marcadé
authors.affiliation: Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Caubet
authors.affiliation: Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France. Electronic address: pierre.greve@univ-poitiers.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Grève
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D003593:Cytoplasm; D004269:DNA, Bacterial / Q000737:chemistry; D054884:Host-Pathogen Interactions; D033321:Isopoda / Q000382:microbiology*; D010802:Phylogeny; D020577:Wolbachia / Q000502:physiology*
chemical_list: D004269:DNA, Bacterial
keywords: Crossings; Cytoplasmic incompatibility; Molecular characterization; Porcellio dilalatatus; Reproductive isolation; Wolbachia