title: Structure of the type IX group B Streptococcus capsular polysaccharide and its evolutionary relationship with types V and VII.
abstract: The Group B Streptococcus capsular polysaccharide type IX was isolated and purified, and the structure of its repeating unit was determined. Type IX capsule → 4)[NeupNAc-α-(2 → 3)-Galp-β-(1 → 4)-GlcpNAc-β-(1 → 6)]-β-GlcpNAc-(1 → 4)-β-Galp-(1 → 4)-β-Glcp-(1 → appears most similar to types VII and V, although it contains two GlcpNAc residues. Genetic analysis identified differences in cpsM, cpsO, and cpsI gene sequences as responsible for the differentiation between the three capsular polysaccharide types, leading us to hypothesize that type V emerged from a recombination event in a type IX background.
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy francesco.berti@novartis.com.
authors.forename: Francesco
authors.initials: F
authors.lastname: Berti
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Edmondo
authors.initials: E
authors.lastname: Campisi
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Toniolo
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Morelli
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Crotti
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Rosini
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Romano
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Vittoria
authors.initials: V
authors.lastname: Pinto
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Brogioni
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Torricelli
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Robert
authors.initials: R
authors.lastname: Janulczyk
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Grandi
authors.affiliation: From Novartis Vaccines Research, 53100 Siena, Italy.
authors.forename: Immaculada
authors.initials: I
authors.lastname: Margarit
mesh_terms: D001483:Base Sequence; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D017931:DNA Primers; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013292:Streptococcus agalactiae / Q000737:chemistry* / Q000235:genetics
chemical_list: D017931:DNA Primers; D011135:Polysaccharides, Bacterial
keywords: Bacterial Genetics; Capsular Polysaccharide; Group B Streptococcus; Nuclear Magnetic Resonance; Polysaccharide; Streptococcus; Vaccine