title: miR-339-5p inhibits cell migration and invasionin vitroand may be associated with the tumor-node-metastasis staging and lymph node metastasis of non-small cell lung cancer.
abstract: The aim of the present study was to investigate the potential role of microRNA (miRNA or miR) in invasion and metastasis of non-small cell lung cancer (NSCLC). miRNA-microarray analysis was used to detect the differentially expressed miRNAs between various metastatic levels of NSCLC cells. The microarray results were verified by quantitative polymerase chain reaction. The most clearly altered miRNA, miR-339-5p, was transfected into NSCLC cells and cell migration and invasion were investigated. The expression of miR-339-5p was 3.4662-fold higher in the lower metastatic NSCLC cells. miR-339-5p significantly decreased tumor-cell migration and the invasion capacityin vitro. In conclusion, miR-339-5p is important in NSCLC invasion and metastasis, indicating that miR-339-5p could be further evaluated as a biomarker for predicting the survival time of patients with NSCLC.
authors.affiliation: Department of Respiratory Medicine, Chinese PLA General Hospital and Chinese PLA Medical School, Beijing 100853, P.R. China ; Department of Respiratory Medicine, The 309th Hospital of Chinese People's Liberation Army, Beijing 100091, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, The 309th Hospital of Chinese People's Liberation Army, Beijing 100091, P.R. China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, The 309th Hospital of Chinese People's Liberation Army, Beijing 100091, P.R. China.
authors.forename: Pengtao
authors.initials: P
authors.lastname: Bao
authors.affiliation: Department of Respiratory Medicine, Chinese PLA General Hospital and Chinese PLA Medical School, Beijing 100853, P.R. China.
authors.forename: Chunsun
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Chinese PLA General Hospital and Chinese PLA Medical School, Beijing 100853, P.R. China.
authors.forename: Xiu Qing
authors.initials: XQ
authors.lastname: Ma
authors.affiliation: Department of Respiratory Medicine, Chinese PLA General Hospital and Chinese PLA Medical School, Beijing 100853, P.R. China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Chinese PLA General Hospital and Chinese PLA Medical School, Beijing 100853, P.R. China.
authors.forename: Liang An
authors.initials: LA
authors.lastname: Chen
keywords: lung carcinoma; lymph node metastasis; microRNAs; tumor-node-metastasis staging