title: Crystallization and preliminary X-ray diffraction studies of La1 from Liocheles australasiae.
abstract: A novel scorpion venom peptide, La1 from Liocheles australasiae, with a molecular weight of 7.8 kDa, is presumed to possess a single von Willebrand factor type C (VWC) domain, a common protein module, based on the position of eight Cys residues in its sequence. The biological function of La1 is still unknown. Deciphering its three-dimensional structure will be helpful in understanding its biological function. La1 was crystallized by the sitting-drop vapour-diffusion method using magnesium sulfate as a precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a=63.0, b=30.2, c=32.3 Å, β=108.5°, and diffracted to 1.9 Å resolution. The calculated VM based on one molecule per asymmetric unit was 1.87 Å3 Da(-1). The solvent content was 34.1%.
authors.affiliation: Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Kamachi
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Nagao
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyashita
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nakagawa
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Miyagawa
authors.affiliation: Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Toshiji
authors.initials: T
authors.lastname: Tada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017434:Protein Structure, Tertiary; D012604:Scorpion Venoms / Q000138:chemical synthesis / Q000737:chemistry*; D012605:Scorpions / Q000737:chemistry* / Q000502:physiology; D060327:Solid-Phase Synthesis Techniques; D014841:von Willebrand Factor / Q000737:chemistry
chemical_list: D060829:Arthropod Proteins; D012604:Scorpion Venoms; D014841:von Willebrand Factor
keywords: La1; Liocheles australasiae; venom peptide