title: Ox-LDL upregulates CRP expression through the IGF2 pathway in THP-1 macrophages.
abstract: C-reactive protein (CRP) is an acute-phase reactant protein that not only plays a predictive role in determining atherogenesis risk but also represents an active participant in atherogenesis onset and progression. Moreover, an increasing number of studies have reported that oxidized low-density lipoprotein (Ox-LDL) plays a significant role in the initiation and progression of atherosclerosis. However, the effect and underlying mechanism of Ox-LDL on CRP expression remains unclear. THP-1 macrophages were treated with 0, 25, 50, or 100 μg/mL of Ox-LDL for 48 h, or 50 μg/mL of Ox-LDL for 0, 12, 24, and 48 h, respectively. Messenger RNA (mRNA) and protein levels were measured by real-time quantitative PCR and Western blot analysis, respectively. We found that Ox-LDL markedly increased insulin-like growth factor 2 (IGF2) and CRP mRNA and protein levels in a dose- and time-dependent manner in THP-1 macrophages. Treatment with Ox-LDL increased CRP protein expression, and this effect was completely abolished by siRNA-mediated silencing of IGF2 in THP-1 macrophages. Moreover, treatment with pcDNA3.1-IGF2 significantly enhanced CRP protein expression in Ox-LDL-stimulated THP-1 macrophages. CRP expression is upregulated by Ox-LDL through the IGF2 pathway in THP-1 macrophages.
authors.affiliation: Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, China.
authors.forename: Shu-Fen
authors.initials: SF
authors.lastname: Li
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Hu
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.forename: Shao-Guo
authors.initials: SG
authors.lastname: Wu
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Lu
authors.forename: Ya-Rong
authors.initials: YR
authors.lastname: Hu
authors.forename: Yan-Chao
authors.initials: YC
authors.lastname: Wang
authors.forename: Ji-Juan
authors.initials: JJ
authors.lastname: Gao
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Sha
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D050197:Atherosclerosis / Q000276:immunology*; D002097:C-Reactive Protein / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000276:immunology / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering
chemical_list: C035170:IGF2 protein, human; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C099252:oxidized low density lipoprotein; D007335:Insulin-Like Growth Factor II; D002097:C-Reactive Protein