title: Pretreatment of cottage cheese to enhance biogas production.
abstract: This study evaluated the possibility of pretreating selected solid fraction of an anaerobic digester treating food waste to lower the hydraulic retention time and increase the methane production. The study investigated the effect of different pretreatments (thermal, chemical, thermochemical and enzymatic) for enhanced methane production from cottage cheese. The most effective pretreatments were thermal and enzymatic. Highest solubilisation of COD was observed in thermal pretreatment, followed by thermochemical. In single enzyme systems, lipase at low concentration gave significantly higher methane yield than for the experiments without enzyme additions. The highest lipase dosages decreased methane yield from cottage cheese. However, in case of protease enzyme an increase in concentration of the enzyme showed higher methane yield. In the case of mixed enzyme systems, pretreatment at 1 : 2 ratio of lipase : protease showed higher methane production in comparison with 1 : 1 and 2 : 1 ratios. Methane production potentials for different pretreatments were as follows: thermal 357 mL/g VS, chemical 293 mL/g VS, and thermochemical 441 mL/g VS. The average methane yield from single enzyme systems was 335 mL/g VS for lipase and 328 mL/g VS for protease. Methane potentials for mixed enzyme ratios were 330, 360, and 339 mL/g VS for 1 : 1, 1 : 2, and 2 : 1 lipase : protease, respectively.
authors.affiliation: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K. K. Birla Goa Campus, Goa 403726, India.
authors.forename: Vidhya
authors.identifier: 0000-0003-0976-3218
authors.initials: V
authors.lastname: Prabhudessai
authors.affiliation: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K. K. Birla Goa Campus, Goa 403726, India.
authors.forename: Bhakti
authors.initials: B
authors.lastname: Salgaonkar
authors.affiliation: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K. K. Birla Goa Campus, Goa 403726, India.
authors.forename: Judith
authors.identifier: 0000-0001-7876-0032
authors.initials: J
authors.lastname: Braganca
authors.affiliation: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K. K. Birla Goa Campus, Goa 403726, India.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Mutnuri
mesh_terms: D000693:Anaerobiosis; D056804:Biofuels; D019149:Bioreactors; D002611:Cheese; D006801:Humans; D008049:Lipase / Q000378:metabolism; D008697:Methane / Q000096:biosynthesis* / Q000737:chemistry; D010447:Peptide Hydrolases / Q000378:metabolism; D012722:Sewage / Q000737:chemistry
chemical_list: D056804:Biofuels; D012722:Sewage; D008049:Lipase; D010447:Peptide Hydrolases; D008697:Methane