title: Phenotypic and molecular characterization of plants regenerated from non-cryopreserved and cryopreserved wild Solanum lycopersicum mill. Seeds.
abstract: BACKGROUND
  Before cryopreservation is routinely used, its effect on the trueness-to-type of the regenerated plant material needs to be evaluated.
  
  
  OBJECTIVE
  In this work, we studied the effect of seed cryopreservation on the phenotypic and molecular characteristics of wild Solanum lycopersicum Mill. plants.
  
  
  METHODS
  Thirty-five morphological traits of plants regenerated from cryopreserved seeds were compared to those measured on plants regenerated from non-cryopreserved seeds.
  
  
  RESULTS
  No statistically significant differences were observed between cryopreserved and non-cryopreserved samples, either in the first or in the second generation post-liquid nitrogen exposure. However, at the molecular level, the genetic analyses performed on the second generation plants germinated from control and cryopreserved seeds using 14 nuclear Simple Sequences Repeats (SSR) markers uncovered some changes in microsatellite length between control and cryopreserved samples. These results confirm at the botanical phenotype level the effectiveness of seed cryostorage for conservation and regeneration of true-to-type S. lycopersicum plants.
  
  
  CONCLUSIONS
  Further experiments are required to clarify potential phenotypic effects of the changes observed in the DNA.
authors.affiliation: Escuela Superior Politecnica Agropecuaria de Manabi Manuel Felix Lopez (ESPAM), Campus Politecnico El Limon, Carrera de Ingenieria Agricola, Calceta, Manabi, Ecuador. bzevallos@espam.edu.ec.
authors.forename: B
authors.initials: B
authors.lastname: Zevallos
authors.affiliation: Faculty of Agronomy, Universidad de Ciego de Avila, Ciego de Avila 69450, Cuba.
authors.forename: I
authors.initials: I
authors.lastname: Cejas
authors.affiliation: IRD, UMR DIADE, Montpellier Cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Engelmann
authors.affiliation: Department of Agricultural Sciences, University of Naples Federico II, Via Universita, Portici (NA), Italy.
authors.forename: D
authors.initials: D
authors.lastname: Carputo
authors.affiliation: Department of Agricultural Sciences, University of Naples Federico II, Via Universita 100 - 80055 Portici (NA), Italy.
authors.forename: R
authors.initials: R
authors.lastname: Aversano
authors.affiliation: Institute of Plant Genetics (Research Division Portici), National Council of Research, Via Universita, Portici (NA), Italy.
authors.forename: M T
authors.initials: MT
authors.lastname: Scarano
authors.affiliation: Laboratory for Plant Breeding, Centro de Bioplantas, Universidad de Ciego de Avila, Ciego de Avila 69450, Cuba.
authors.forename: E
authors.initials: E
authors.lastname: Yanes
authors.affiliation: Laboratory for Plant Breeding, Centro de Bioplantas, Universidad de Ciego de Avila, Ciego de Avila 69450, Cuba.
authors.forename: M
authors.initials: M
authors.lastname: Martinez-Montero
authors.affiliation: Laboratory for Plant Breeding, Centro de Bioplantas, Universidad de Ciego de Avila, Ciego de Avila 69450, Cuba.
authors.forename: J C
authors.initials: JC
authors.lastname: Lorenzo
mesh_terms: D015925:Cryopreservation; D018744:DNA, Plant / Q000235:genetics; D018551:Solanum lycopersicum / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development*; D018895:Microsatellite Repeats; D010641:Phenotype; D012639:Seeds / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development*
chemical_list: D018744:DNA, Plant