title: Preparation of pH sensitive insulin-loaded nano hydrogels and evaluation of insulin releasing in different pH conditions.
abstract: In the recent years, temperature and pH-sensitive hydrogels were developed as suitable carriers for drug delivery. In this study, four different pH-sensitive nanohydrogels were designed for an oral insulin delivery modeling. NIPAAm-MAA-HEM copolymers were synthesized by radical chain reaction with 80:8:12 ratios respectively. Reactions were carried out in four conditions including 1,4-dioxan and water as two distinct solution under nitrogen gas-flow. The copolymers were characterized with FT-IR, SEM and TEM. Copolymers were loaded with regular insulin by modified double emulsion method with ratio of 1:10. Release study carried out in pH 1.2 and pH 6.8 at 37 °C. For pH 6.8 and pH 1.2, 2 mg of the insulin loaded nanohydrogels was float in a beaker containing 100 mL of PBS with pH 6.8 and 100 mL of HCl solution with pH 1.2, respectively. Sample collection was done in different times and HPLC was used for analysis of samples using water/acetonitrile (65/35) as the mobile phase. Nanohydrogels synthesis reaction yield was 95 %, HPLC results showed that loading in 1,4-dioxan without cross-linker nanohydrogels was more than others, also indicated that the insulin release of 1,4-dioxan without cross-linker nanohydrogels at acidic pH is less, but in pH 6.8 is the most. Results showed that by opting suitable polymerization method and selecting the best nanohydrogels, we could obtain a suitable insulin loaded nanohydrogels for oral administration.
authors.affiliation: Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Jalil
authors.initials: J
authors.lastname: Karnoosh-Yamchi
authors.forename: Majid
authors.initials: M
authors.lastname: Mobasseri
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Akbarzadeh
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Davaran
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Ostad-Rahimi
authors.forename: Hamed
authors.initials: H
authors.lastname: Hamishehkar
authors.forename: Roya
authors.initials: R
authors.lastname: Salehi
authors.forename: Zahra
authors.initials: Z
authors.lastname: Bahmani
authors.forename: Kazem
authors.initials: K
authors.lastname: Nejati-Koshki
authors.forename: Akbar
authors.initials: A
authors.lastname: Darbin
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rahmati-Yamchi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D016503:Drug Delivery Systems; D065546:Drug Liberation; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000008:administration & dosage*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D020100:Hydrogels; D007328:Insulin; D011108:Polymers