title: Healing of stoma after magnetic biliary-enteric anastomosis in canine peritonitis models.
abstract: OBJECTIVE
  To assese the healing of stoma after magnetic anastomosis for the reconstruction of biliary-enteric continuity under severe inflammation.
  
  
  METHODS
  Acute bile duct injury was constructed as a bile peritonitis model in mongrel dogs (n=32). Magnetic anastomosis (group A, n=16) and traditional suture anastomosis (group B, n=16) were performed to reconstruct the biliary-enteric continuity in one stage. Half of the dogs in each group were euthanized on the 30th postoperative day, and the other half on the 90th postoperative day to harvest the stoma region. The healing conditions of the stoma after the 2 anastomotic approaches were observed with naked eyes, under light microscope and scanning electron microscope.
  
  
  RESULTS
  The stoma leakage rate (50% versus 0% on the 30th postoperative day, 37.5% versus 12.5% on the 90th postoperative day, both P<0.05) and stenosis degree (13.9%±0.3% versus 7.1%±0.3% on the 30th postoperative day, 17.2%±0.4% versus 9.4%±0.4% on the 90th postoperative day, both P<0.01) were significantly higher in group B than in group A. Compared with traditional manual anastomoses, the histological analysis under light and electron microscope showed a more continuous stoma with more regular epithelium proliferation and collagen arrangement, less inflammation in group A.
  
  
  CONCLUSIONS
  Magnetic anastomosis stent ensures better healing of the stoma even under the circumstance of severe inflammation.
authors.affiliation: 1.Department of Surgical Oncology, Shaanxi Provincial People's Hospital (The Third Affiliated Hospital of the School of Medicine Xi'an Jiaotong University), Xi'an 710068, China;2.Research Institute of Advanced Surgical Technology and Engineering, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Li
authors.affiliation: Research Institute of Advanced Surgical Technology and Engineering, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Long
authors.initials: L
authors.lastname: Guo
authors.affiliation: 1.Department of Surgical Oncology, Shaanxi Provincial People's Hospital (The Third Affiliated Hospital of the School of Medicine Xi'an Jiaotong University), Xi'an 710068, China;2.Research Institute of Advanced Surgical Technology and Engineering, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Wei-Jie
authors.initials: WJ
authors.lastname: Yao
authors.affiliation: Department of Surgical Oncology, Shaanxi Provincial People's Hospital (The Third Affiliated Hospital of the School of Medicine Xi'an Jiaotong University), Xi'an 710068, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zhang
authors.affiliation: Research Institute of Advanced Surgical Technology and Engineering, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Shan-Pei
authors.initials: SP
authors.lastname: Wang
authors.affiliation: Research Institute of Advanced Surgical Technology and Engineering, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Shi-Qi
authors.initials: SQ
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Geng
authors.affiliation: Research Institute of Advanced Surgical Technology and Engineering, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Song
authors.affiliation: 2.Research Institute of Advanced Surgical Technology and Engineering, Xi'an Jiaotong University, Xi'an 710061, China;3.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lv
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001652:Bile Ducts / Q000601:surgery*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D007422:Intestines / Q000601:surgery*; D008280:Magnetics; D008297:Male; D008855:Microscopy, Electron, Scanning; D010538:Peritonitis / Q000601:surgery*; D054047:Surgical Stomas; D014945:Wound Healing