title: Tissue processing and hematoxylin and eosin staining.
abstract: The hematoxylin and eosin (H&E) stained tissue section is the cornerstone of anatomical pathology diagnosis. The H&E procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components. However, staining results are dependent on proper specimen processing, which involves tissue preservation, dehydration, clearing, and paraffin infiltration. While improvements in instrumentation for both tissue processing and staining have been beneficial, limitations in the chemical reagents used must always be considered.
authors.affiliation: Anatech Ltd., 1020 Harts Lake Road, Battle Creek, MI, 49037, USA, adafeldman@anatechltdusa.com.
authors.forename: Ada T
authors.initials: AT
authors.lastname: Feldman
authors.forename: Delia
authors.initials: D
authors.lastname: Wolfe
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D004801:Eosine Yellowish-(YS) / Q000378:metabolism*; D006416:Hematoxylin / Q000378:metabolism*; D012015:Reference Standards; D013048:Specimen Handling; D013194:Staining and Labeling / Q000379:methods* / Q000592:standards
chemical_list: D004801:Eosine Yellowish-(YS); D006416:Hematoxylin