title: Enhanced bioelectricity generation by improving pyocyanin production and membrane permeability through sophorolipid addition in Pseudomonas aeruginosa-inoculated microbial fuel cells.
abstract: Improvement on electron shuttle-mediated extracellular electron transfer (EET) is of great potential to enhance the power output of MFCs. In this study, sophorolipid was added to enhance the performance of Pseudomonas aeruginosa-inoculated MFC by improving the electron shuttle-mediated EET. Upon sophorolipid addition, the current density and power density increased ∼ 1.7 times and ∼ 2.6 times, respectively. In accordance, significant enhancement on pyocyanin production (the electron shuttle) and membrane permeability were observed. Furthermore, the conditions for sophorolipid addition were optimized to achieve maximum pyocyanin production (14.47 ± 0.23 μg/mL), and 4 times higher power output was obtained compared to the control. The results substantiated that enhanced membrane permeability and pyocyanin production by sophorolipid, which promoted the electron shuttle-mediated EET, underlies the improvement of the energy output in the P. aeruginosa-inoculated MFC. It suggested that addition of biosurfactant could be a promising way to enhance the energy generation in MFCs.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing TECH University, Nanjing 210009, China; Bioenergy Research Institute, Nanjing TECH University, Nanjing 210009, China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Shen
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing TECH University, Nanjing 210009, China; Bioenergy Research Institute, Nanjing TECH University, Nanjing 210009, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Yong
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing TECH University, Nanjing 210009, China.
authors.forename: Yi-Lu
authors.initials: YL
authors.lastname: Chen
authors.affiliation: Biofuels Institute, School of the Environment, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Liao
authors.affiliation: Biofuels Institute, School of the Environment, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Rong-Wei
authors.initials: RW
authors.lastname: Si
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing TECH University, Nanjing 210009, China; Bioenergy Research Institute, Nanjing TECH University, Nanjing 210009, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing TECH University, Nanjing 210009, China; Bioenergy Research Institute, Nanjing TECH University, Nanjing 210009, China.
authors.forename: Shu-Ya
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Biofuels Institute, School of the Environment, Jiangsu University, Zhenjiang 212013, China. Electronic address: ycyong@mail.ujs.edu.cn.
authors.forename: Yang-Chun
authors.initials: YC
authors.lastname: Yong
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing TECH University, Nanjing 210009, China; Bioenergy Research Institute, Nanjing TECH University, Nanjing 210009, China.
authors.forename: Ping-Kai
authors.initials: PK
authors.lastname: OuYang
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing TECH University, Nanjing 210009, China; Bioenergy Research Institute, Nanjing TECH University, Nanjing 210009, China. Electronic address: zhengtao@njtech.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Zheng
mesh_terms: D001674:Bioelectric Energy Sources / Q000382:microbiology*; D002463:Cell Membrane Permeability; D002851:Chromatography, High Pressure Liquid; D004560:Electricity; D055664:Electrochemical Techniques; D004566:Electrodes; D004583:Electrons; D006017:Glycolipids / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011710:Pyocyanine / Q000096:biosynthesis*; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D013501:Surface-Active Agents; D011710:Pyocyanine
keywords: Microbial fuel cell (MFC); Permeability; Pseudomonas aeruginosa; Pyocyanin; Sophorolipid