title: In vivocorneal neovascularization imaging by optical-resolution photoacoustic microscopy.
abstract: Corneal neovascularization leads to blurred vision, thusin vivovisualization is essential for pathological studies in animal models. Photoacoustic (PA) imaging can delineate microvasculature and hemodynamics noninvasively, which is suitable for investigating corneal neovascularization. In this study, we demonstratein vivoimaging of corneal neovascularization in the mouse eye by optical-resolution photoacoustic microscopy (OR-PAM), where corneal neovascularization is induced by deliberate alkali burn injuries in C57BL6/J inbred mice corneas on the left eye. We used OR-PAM to image five mice with corneal alkali burn injuries; the uninjured eyes (right eye) in these mice are then used as the controls. Corneal images acquired by OR-PAM with and without alkali burn injury are compared, clear signs of corneal neovascularization are present in the OR-PAM images of injured eyes; the OR-PAM results are also confirmed by postmortem fluorescence-labeled confocal microscopy.
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Liu
authors.affiliation: Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Kathryn M
authors.initials: KM
authors.lastname: Schultz
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Sasman
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA ; State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China.
authors.forename: Fengli
authors.initials: F
authors.lastname: Gao
authors.affiliation: Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kume
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA ; Department of Ophthalmology, Northwestern University, Chicago, IL, 60611, USA.
authors.forename: Hao F
authors.initials: HF
authors.lastname: Zhang
keywords: cornea; neovascularization; photoacoustic microscopy