title: Up-regulating pyocyanin production by amino acid addition for early electrochemical identification of Pseudomonas aeruginosa.
abstract: This work focuses on developing a faster method for electrochemically detecting a Pseudomonas aeruginosa infection through the addition of amino acids to cell culture samples. We performed square-wave voltammetry measurements of pyocyanin produced by P. aeruginosa using commercially available carbon-based electrodes connected to a Ag/AgCl reference. The electrochemical response resulting from the production of pyocyanin by bacteria was measured in the presence of various amino acids while varying three different culturing parameters: liquid media type (trypticase soy broth vs. M63 minimal media); concentration of amino acids in the solution; and initial concentration of the P. aeruginosa in the solution. Our results demonstrate a faster and stronger electrochemical response in media containing tyrosine and valine at elevated concentrations, lending promise to using amino acids as up-regulatory molecules for faster bacterial detection.
authors.affiliation: Department of Chemical Engineering, Northeastern University, 360 Huntington Ave, 313 Snell Engineering, Boston, MA 02115, USA. e.goluch@neu.edu.
authors.forename: Hunter J
authors.initials: HJ
authors.lastname: Sismaet
authors.forename: Thaddaeus A
authors.initials: TA
authors.lastname: Webster
authors.forename: Edgar D
authors.initials: ED
authors.lastname: Goluch
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D011552:Pseudomonas Infections / Q000175:diagnosis / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification* / Q000378:metabolism*; D011710:Pyocyanine / Q000032:analysis / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D003470:Culture Media; D011710:Pyocyanine