title: DNA barcodes for the fishes of the Narmada, one of India's longest rivers.
abstract: This study describes the species diversity of fishes of the Narmada River in India. A total of 820 fish specimens were collected from 17 sampling locations across the whole river basin. Fish were taxonomically classified into one of 90 possible species based on morphological characters, and then DNA barcoding was employed using COI gene sequences as a supplemental identification method. A total of 314 different COI sequences were generated, and specimens were confirmed to belong to 85 species representing 63 genera, 34 families and 10 orders. Findings of this study include the identification of five putative cryptic or sibling species and 43 species not previously known from the Narmada River basin. Five species are endemic to India and three are introduced species that had not been previously reported to occur in the Narmada River. Conversely, 43 species previously reported to occur in the Narmada were not found. Genetic diversity and distance values were generated for all of the species within genera, families and orders using Kimura's 2 parameter distance model followed by the construction of a Neighbor Joining tree. High resolution clusters generated in NJ trees aided the groupings of species corresponding to their genera and families which are in confirmation to the values generated by Automatic Barcode Gap Discovery bioinformatics platform. This aided to decide a threshold value for the discrimination of species boundary from the Narmada River. This study provides an important validation of the use of DNA barcode sequences for monitoring species diversity and changes within complex ecosystems such as the Narmada River.
authors.affiliation: Paul Hebert Centre for DNA Barcoding and Biodiversity Studies, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India.
authors.forename: Gulab Dattarao
authors.initials: GD
authors.lastname: Khedkar
authors.affiliation: Paul Hebert Centre for DNA Barcoding and Biodiversity Studies, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Jamdade
authors.affiliation: Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Suresh
authors.initials: S
authors.lastname: Naik
authors.affiliation: Department of Animal Sciences, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
authors.forename: Lior
authors.initials: L
authors.lastname: David
authors.affiliation: Department of Cell and Molecular Biology, University of Hawaii, Honolulu, Hawaii, United States of America.
authors.forename: David
authors.initials: D
authors.lastname: Haymer
mesh_terms: D000818:Animals; D019295:Computational Biology; D058893:DNA Barcoding, Taxonomic; D030541:Databases, Genetic; D005399:Fishes / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D007194:India; D010802:Phylogeny; D045483:Rivers; D017422:Sequence Analysis, DNA