title: Spatial proteomic and phospho-proteomic organization in three prototypical cell migration modes.
abstract: BACKGROUND
  Tight spatio-temporal signaling of cytoskeletal and adhesion dynamics is required for localized membrane protrusion that drives directed cell migration. Different ensembles of proteins are therefore likely to get recruited and phosphorylated in membrane protrusions in response to specific cues.
  
  
  RESULTS
  HERE, WE USE AN ASSAY THAT ALLOWS TO BIOCHEMICALLY PURIFY EXTENDING PROTRUSIONS OF CELLS MIGRATING IN RESPONSE TO THREE PROTOTYPICAL RECEPTORS: integrins, recepor tyrosine kinases and G-coupled protein receptors. Using quantitative proteomics and phospho-proteomics approaches, we provide evidence for the existence of cue-specific, spatially distinct protein networks in the different cell migration modes.
  
  
  CONCLUSIONS
  The integrated analysis of the large-scale experimental data with protein information from databases allows us to understand some emergent properties of spatial regulation of signaling during cell migration. This provides the cell migration community with a large-scale view of the distribution of proteins and phospho-proteins regulating directed cell migration.
authors.affiliation: ETH Zurich, D-BSSE, Mattenstrasse 26, CH-4058 Basel, Switzerland.
authors.forename: Georgios
authors.initials: G
authors.lastname: Fengos
authors.affiliation: University of Basel, Biozentrum, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.
authors.forename: Alexander
authors.initials: A
authors.lastname: Schmidt
authors.affiliation: Department Biomedicine, University of Basel, Mattenstrasse 28, CH-4058 Basel, Switzerland.
authors.forename: Katrin
authors.initials: K
authors.lastname: Martin
authors.affiliation: Department Biomedicine, University of Basel, Mattenstrasse 28, CH-4058 Basel, Switzerland.
authors.forename: Erika
authors.initials: E
authors.lastname: Fluri
authors.affiliation: ETH Zurich, Department of Biology, Institute of Molecular Systems Biology, Wolfgang-Pauli-Strasse 16, CH-8093 Zurich, Switzerland ; University of Zurich, Faculty of Science, Zurich, Switzerland.
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
authors.affiliation: ETH Zurich, D-BSSE, Mattenstrasse 26, CH-4058 Basel, Switzerland.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Iber
authors.affiliation: Department Biomedicine, University of Basel, Mattenstrasse 28, CH-4058 Basel, Switzerland.
authors.forename: Olivier
authors.initials: O
authors.lastname: Pertz
keywords: Directional cell migration; Fibroblast; Phosphorylation; Proteomics; Signaling