title: αB-crystallin and HSP27 in glial cells in tauopathies.
abstract: Tauopathies are neurodegenerative diseases characterized by hyper-phosphorylated tau deposition in neurons and glial cells. Chaperones, such as small heat shock proteins αB-crystallin and HSP27 highly expressed in normal glial cells, have been postulated as putative molecules preventing abnormal deposition and folding in glial cells in tauopathies. The objective of this work was to assess the expression of αB-crystallin, phosphorylated αB-crystallin at Ser59 and HSP27 in glial cells with and without tau deposits in progressive supranuclear palsy, corticobasal degeneration (CBD), argyrophilic grain disease (AGD), Pick's disease (PiD), Alzheimer's disease, frontotemporal lobar degeneration associated with mutations in the tau gene (FTLD-tau), globular glial tauopathy (GGT) and tauopathy in the elderly. Immunohistochemistry, and double-labeling immunofluorescence and confocal microscopy have been used for this purpose. Increased expression of αB-crystallin and phosphorylated αB-crystallin at Ser59 occurs in a subpopulation of glial cells with and without hyper-phosphorylated tau deposition in all the analyzed tauopathies, but their expression in neurons is restricted to ballooned neurons in CBD, AGD and PiD. HSP27 barely co-localizes with tau and with phosphorylated αB-crystallin at Ser59, thus making the formation of active dimers operating as chaperones unlikely. Results suggest a limited function of αB-crystallin and HSP27 in preventing abnormal tau protein deposition in glial cells and neurons; in addition, the expression of αB-crystallin phosphorylated at Ser59 may act as a protective factor in glial cells.
authors.affiliation: Institut de Neuropatologia, IDIBELL-Hospital Universitari de Bellvitge, Hospitalet de Llobregat, CIBERNED (Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas), Universitat de Barcelona, Barcelona, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: López-González
authors.forename: Margarita
authors.initials: M
authors.lastname: Carmona
authors.forename: Laura
authors.initials: L
authors.lastname: Arregui
authors.forename: Gabor G
authors.initials: GG
authors.lastname: Kovacs
authors.forename: Isidre
authors.initials: I
authors.lastname: Ferrer
mesh_terms: D001921:Brain / Q000378:metabolism*; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006801:Humans; D018832:Molecular Chaperones; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010766:Phosphorylation; D024801:Tauopathies / Q000378:metabolism*; D038203:alpha-Crystallin B Chain / Q000378:metabolism*; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D038203:alpha-Crystallin B Chain; D016875:tau Proteins
keywords: HSP27; astrocytes; oligodendrocytes; tauopathies; αB-crystallin