title: Regulatory B cells in mouse models of systemic lupus erythematosus (SLE).
abstract: Regulatory B cells are now recognized as an essential component of the immune system. The function of regulatory B cells is dependent on IL-10. The cell-surface phenotype of murine IL-10-producing regulatory B cells is reported to be CD1d(hi)CD5(+) B cell or CD21(hi)CD23(hi) T2 marginal zone precursor B cells. B cells play several critical roles in the pathogenesis of systemic lupus erythematosus (SLE). It is now apparent that regulatory B cells are important for disease suppression in SLE. Regulatory B cells inhibit disease onset of NZB/W F1 mice, a spontaneous SLE mouse model. Furthermore, the potential therapeutic effect of regulatory B cells in NZB/W F1 mice is highlighted by the adoptive transfer of splenic CD1d(hi)CD5(+) B cells. Regulatory B cells also suppress the disease manifestation in MRL-Fas(lpr) mice, a SLE mouse model. Thus, regulatory B cells have protective role and therapeutic effects in mouse modes of SLE. Herein, the methods for evaluating SLE mouse model, B-cell depletion, and regulatory B-cell analysis are provided. These methods should facilitate the study of regulatory B cells in SLE.
authors.affiliation: Department of Dermatology, Faculty of Medicine, Institute of Medical, Pharmaceutical, and Health Sciences, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-8641, Japan, t-matsushita@med.kanazawa-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsushita
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis; D060151:B-Lymphocytes, Regulatory / Q000276:immunology* / Q000473:pathology*; D004195:Disease Models, Animal; D006801:Humans; D007668:Kidney / Q000276:immunology / Q000473:pathology; D007674:Kidney Diseases / Q000150:complications / Q000175:diagnosis / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology*; D008814:Mice, Inbred NZB / Q000276:immunology*; D011507:Proteinuria / Q000150:complications / Q000175:diagnosis
chemical_list: D000974:Antibodies, Antinuclear