title: Cell growth kinetics of Chlorella sorokiniana and nutritional values of its biomass.
abstract: The present study investigates the effects of different physico-chemical parameters for the growth of Chlorella sorokiniana and subsequently determination of nutritional values of its biomass. Most suitable temperature, light intensity, pH, and acetic acid concentration were 30°C, 100 μmol m(-2)s(-1), pH 7.5, and 34.8mM, respectively for the growth of this microorganism. Arrhenius growth activation energy, Ea was calculated as 7.08 kJ mol(-1). Monod kinetics constants: maximum specific growth rate (μ max) and substrate (acetic acid) affinity coefficient (Ks) were determined as 0.1 ± 0.01 h(-1) and 76 ± 8 mg L(-1), respectively. Stoichiometric analysis revealed the capture of 1.83 g CO2 and release of 1.9 g O2 for 1g algal biomass synthesis. Algal biomass of C. sorokiniana was found rich in protein and several important minerals such as Mg, Ca, and Fe. Astaxanthin and β-carotene were extracted and quantified using high performance liquid chromatography.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Kanhaiya
authors.initials: K
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
authors.forename: Chitralekha Nag
authors.initials: CN
authors.lastname: Dasgupta
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India. Electronic address: ddas.iitkgp@gmail.com.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Das
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D018533:Biomass; D002244:Carbon / Q000032:analysis; D002708:Chlorella / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects; D003470:Culture Media / Q000737:chemistry; D006859:Hydrogen / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D008903:Minerals / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D009753:Nutritive Value; D010860:Pigments, Biological / Q000302:isolation & purification; D013455:Sulfur / Q000032:analysis; D013696:Temperature
chemical_list: D003470:Culture Media; D008903:Minerals; D010860:Pigments, Biological; D013455:Sulfur; D002244:Carbon; D006859:Hydrogen; D009584:Nitrogen; D019342:Acetic Acid
keywords: Elemental analysis; Light; Pigment extraction; Temperature; pH