title: Changes in gene expression profile in two multidrug resistant cell lines derived from a same drug sensitive cell line.
abstract: Resistance to chemotherapy is one of the most relevant aspects of treatment failure in cancer. Cell lines are used as models to study resistance. We analyzed the transcriptional profile of two multidrug resistant (MDR) cell lines (Lucena 1 and FEPS) derived from the same drug-sensitive cell K562. Microarray data identified 130 differentially expressed genes (DEG) between K562 vs. Lucena 1, 1932 between K562 vs. FEPS, and 1211 between Lucena 1 versus FEPS. The NOTCH pathway was affected in FEPS with overexpression of NOTCH2 and HEY1. The highly overexpressed gene in MDR cell lines was ABCB1, and both presented the ABCB1 promoter unmethylated.
authors.affiliation: Genetics Program, Instituto Nacional de Câncer, Rio de Janeiro, Brazil. Electronic address: miguelm@inca.gov.br.
authors.forename: Miguel Angelo Martins
authors.initials: MA
authors.lastname: Moreira
authors.affiliation: Genetics Program, Instituto Nacional de Câncer, Rio de Janeiro, Brazil; Genetics Department, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Bagni
authors.affiliation: Genetics Program, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
authors.forename: Marcos Barcelos
authors.initials: MB
authors.lastname: de Pinho
authors.affiliation: Genetics Program, Instituto Nacional de Câncer, Rio de Janeiro, Brazil; Genetics Department, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Thaís Messias
authors.initials: TM
authors.lastname: Mac-Cormick
authors.affiliation: Genetics Program, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
authors.forename: Mateus
authors.initials: M
authors.lastname: dos Santos Mota
authors.affiliation: Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Flávio Eduardo
authors.initials: FE
authors.lastname: Pinto-Silva
authors.affiliation: Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Nathalia
authors.initials: N
authors.lastname: Daflon-Yunes
authors.affiliation: Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Vivian Mary
authors.initials: VM
authors.lastname: Rumjanek
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D045744:Cell Line, Tumor; D019175:DNA Methylation; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D046228:Microarray Analysis; D011401:Promoter Regions, Genetic; D059467:Transcriptome / Q000187:drug effects
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1
keywords: ABCB transporters; ABCB1; Cancer; MDR cell lines; Methylation; Multidrug resistance