title: Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
abstract: Pig pancreas may be a therapeutic resource for human diabetic patients. However, this potential is hindered by a lack of knowledge of the molecular events of pig pancreas development. In this study, the embryonic day 60, neonate and 6-month protein profiles of pig pancreas were ascertained at using two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry. Twenty four proteins were differentially expressed during pig pancreas development. Among them, 12 spots increased and 7 spots decreased according to development. The expression of 5 protein were highest at birth. Expression of digestive enzymes including trypsin, pancreatic triacylglycerol lipase and pancreatic alpha-amylase was elevated in adults, whereas chymotrypsins were highly expressed in neonates. Proteins that were abundantly expressed during gestation were alpha-1-antitrypsin, alpha-fetoprotein and transferrins. Taken together, we found out that several proteins were significantly up- or down- regulated from pig pancreas based on developmental stage. This study will provide basis for understanding development of pig pancreas.
authors.affiliation: Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21Plus Program for Creative Veterinary Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
authors.forename: Ji Ye
authors.initials: JY
authors.lastname: Ahn
authors.affiliation: Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21Plus Program for Creative Veterinary Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
authors.forename: Il Yong
authors.initials: IY
authors.lastname: Kim
authors.affiliation: Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21Plus Program for Creative Veterinary Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
authors.forename: Sae Jin
authors.initials: SJ
authors.lastname: Oh
authors.affiliation: Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21Plus Program for Creative Veterinary Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
authors.forename: Hye Sook
authors.initials: HS
authors.lastname: Hwang
authors.affiliation: Department of Biomedical Laboratory Science, College of Biomedical Science, Soonchunhyang University, Asan, Korea.
authors.forename: Sun Shin
authors.initials: SS
authors.lastname: Yi
authors.affiliation: Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21Plus Program for Creative Veterinary Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
authors.forename: Yo Na
authors.initials: YN
authors.lastname: Kim
authors.affiliation: Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21Plus Program for Creative Veterinary Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21Plus Program for Creative Veterinary Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
authors.forename: Yeo Sung
authors.initials: YS
authors.lastname: Yoon
authors.affiliation: Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21Plus Program for Creative Veterinary Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
authors.forename: Je Kyung
authors.initials: JK
authors.lastname: Seong
keywords: 2-DE proteomics; Domestic pig; MALDI-TOF; development; pancreas