title: [Role of BCL-2, caspase-3 and NF-κB in astragaloside inducing apoptosis of human NB4 cells].
abstract: This study was purposed to investigate the apoptosis-inducing effect of astragalosides on acute promyelocytic leukemia(APL) cell line NB4 and its mechanism. NB4 cells were treated with different concentrations (200, 300, 400 µg /ml) of astragalosides for 48 h. The cell proliferation was assayed by using CCK-8 method; the cell apoptosis was analyzed by flow cytometry with Annexin V-FITE/PI double staining. The mRNA expression of BCL-2 and the relative activity of BCL-2, NF-κB and caspase-3 were detected by RT-PCR and Western blot, respectively. The results showed that after treated with astragalosides for 48 h, astragalosides inhibited NB4 cell proliferation in concentration-dependent way, the apoptosis rate of NB4 cells gradually elevated from 4.69% to 40.85% with the increasing of astragalosides concentration. Simultaneously, the mRNA expression of BCL-2 was down-regulated, Western blot analysis showed that the protein expression levels of BCL-2 and NF-κB decreased after astragalosides treatment, while caspase-3 protein expression level increased. It is concluded that the molecular mechanism of the astragalosides-induced apoptosis in NB4 cells may be associated with down-regulation of the expression of BCL-2 and NF-κB, finally the relative activity of caspase-3 activated.
authors.affiliation: Department of Homatology, The Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ni
authors.affiliation: Department of Homatology, The Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Homatology, The Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Homatology, The Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China.
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Hu
authors.affiliation: Department of Homatology, The Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China.
authors.forename: Zhen-Qi
authors.initials: ZQ
authors.lastname: Huang
authors.affiliation: Department of Homatology, The Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China. E-mail: xrx2041@163.com.
authors.forename: Rui-Xiang
authors.initials: RX
authors.lastname: Xia
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012503:Saponins / Q000494:pharmacology*
chemical_list: D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D012503:Saponins; C505407:CASP3 protein, human; D053148:Caspase 3