title: [Effect of COX-2 inhibitor celecoxib on proliferation, apoptosis of HL-60 cells and its mechanism].
abstract: This study was aimed to investigate the effect of COX-2 inhibitor celecoxib on proliferation, apoptosis of human acute myeloid leukemia cell line HL-60 and its mechanism. HL-60 cells were cultured with different concentrations of celecoxib for 24 h. Cell proliferation was analyzed by CCK-8 assay, cell apoptosis and cell cycle distribution were detected by flow cytometry. Cyclin D1, cyclin E1 and COX-2 mRNA expressions were determined by RT-PCR. The results showed that after the HL-60 cells were treated with different concentrations of celecoxib for 24 h, the cell growth was significantly inhibited in a dose-dependent manner(r = 0.955), IC50 was 63.037 µmol/L of celecoxib. Celecoxib could effectively induce apoptosis in HL-60 cells also in dose-dependent manner(r = 0.988), blocked the HL-60 cells in the G0/G1 phase. The expression of cyclin D1, cyclin E1 and COX-2 mRNA were downregulated. It is concluded that celecoxib can inhibit the proliferation of HL-60 cells in dose-dependent manner, celecoxib causes cell G0/G1 arrest and induces cell apoptosis possibly through down-regulation of the cyclin D1 and cyclin E1 expression, and down-regulation of COX-2 expression respectively.
authors.affiliation: Department of Hematology, Hebei Provincial People's Hospital, Shijiazhuang 050051, Hebei Province, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Hematology, Hebei Provincial People's Hospital, Shijiazhuang 050051, Hebei Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Hematology, Hebei Provincial People's Hospital, Shijiazhuang 050051, Hebei Province, China.
authors.forename: Rui-Cang
authors.initials: RC
authors.lastname: Wang
authors.affiliation: Department of Hematology, Hebei Provincial People's Hospital, Shijiazhuang 050051, Hebei Province, China.
authors.forename: Rui-Li
authors.initials: RL
authors.lastname: Geng
authors.affiliation: Department of Hematology, Hebei Provincial People's Hospital, Shijiazhuang 050051, Hebei Province, China.
authors.forename: Su-Yun
authors.initials: SY
authors.lastname: Wang
authors.affiliation: Department of Hematology, Hebei Provincial People's Hospital, Shijiazhuang 050051, Hebei Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Hematology, Hebei Provincial People's Hospital, Shijiazhuang 050051, Hebei Province, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Hebei Provincial People's Hospital, Shijiazhuang 050051, Hebei Province, China. E-mail: h0707@163.com.
authors.forename: Hong-Ling
authors.initials: HL
authors.lastname: Hao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D000068579:Celecoxib; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1 / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D015973:Gene Expression Regulation, Leukemic; D018922:HL-60 Cells; D006801:Humans; D015513:Oncogene Proteins / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*
chemical_list: C530104:CCND1 protein, human; C475820:CCNE1 protein, human; D019927:Cyclin E; D052246:Cyclooxygenase 2 Inhibitors; D015513:Oncogene Proteins; D011720:Pyrazoles; D013449:Sulfonamides; D019938:Cyclin D1; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D000068579:Celecoxib