title: Biosorption of nickel by Lysinibacillus sp. BA2 native to bauxite mine.
abstract: The current scenario of environmental pollution urges the need for an effective solution for toxic heavy metal removal from industrial wastewater. Bioremediation is the most cost effective process employed by the use of microbes especially bacteria resistant to toxic metals. In this study, Lysinibacillus sp. BA2, a nickel tolerant strain isolated from bauxite mine was used for the biosorption of Ni(II). Lysinibacillus sp. BA2 biomass had isoelectric point (pI) of 3.3. The maximum negative zeta potential value (-39.45) was obtained at pH 6.0 which was highly favourable for Ni(II) biosorption. 238.04mg of Ni(II) adsorbed on one gram of dead biomass and 196.32mg adsorbed on one gram of live biomass. The adsorption of Ni(II) on biomass increased with time and attained saturation after 180min with rapid biosorption in initial 30min. The Langmuir and Freundlich isotherms could fit well for biosorption of Ni(II) by dead biomass while Langmuir isotherm provided a better fit for live biomass based on correlation coefficient values. The kinetic studies of Ni(II) removal, using dead and live biomass was well explained by second-order kinetic model. Ni(II) adsorption on live biomass was confirmed by SEM-EDX where cell aggregation and increasing irregularity of cell morphology was observed even though cells were in non-growing state. The FTIR analysis of biomass revealed the presence of carboxyl, hydroxyl and amino groups, which seem responsible for biosorption of Ni(II). The beads made using dead biomass of Lysinibacillus sp. BA2 could efficiently remove Ni(II) from effluent solutions. These microbial cells can substitute expensive methods for treating nickel contaminated industrial wastewaters.
authors.affiliation: Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune 411033, India.
authors.forename: Desale
authors.initials: D
authors.lastname: Prithviraj
authors.affiliation: Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune 411033, India.
authors.forename: Kashyap
authors.initials: K
authors.lastname: Deboleena
authors.affiliation: Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune 411033, India. Electronic address: neelunawani@yahoo.com.
authors.forename: Nawani
authors.initials: N
authors.lastname: Neelu
authors.affiliation: School of Bioscience, University of Skövde, PO Box 408, 541 28 Skövde, Sweden.
authors.forename: Nahar
authors.initials: N
authors.lastname: Noor
authors.affiliation: School of Bioscience, University of Skövde, PO Box 408, 541 28 Skövde, Sweden.
authors.forename: Rahman
authors.initials: R
authors.lastname: Aminur
authors.affiliation: Department of Microbiology, University of Pune, Pune 411007, India.
authors.forename: Kapadnis
authors.initials: K
authors.lastname: Balasaheb
authors.affiliation: School of Bioscience, University of Skövde, PO Box 408, 541 28 Skövde, Sweden.
authors.forename: Mandal
authors.initials: M
authors.lastname: Abul
mesh_terms: D000327:Adsorption; D000537:Aluminum Oxide; D001406:Bacillaceae / Q000737:chemistry / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D018533:Biomass; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008906:Mining; D009532:Nickel / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D062065:Wastewater
chemical_list: D000537:Aluminum Oxide; D009532:Nickel; D062065:Wastewater
keywords: Adsorption isotherm; Biosorption; Heavy metals; Lysinibacillus sp. BA2