title: MPT64 protein from Mycobacterium tuberculosis inhibits apoptosis of macrophages through NF-kB-miRNA21-Bcl-2 pathway.
abstract: MPT64 is one of the secreted proteins from Mycobacterium tuberculosis. Little is known about its role in infection by Mycobacterium tuberculosis. In this study, we demonstrated that MPT64 could dose-dependently inhibit the apoptosis of RAW264.7 macrophages induced by PPD-BCG. Quantitative real-time PCR results showed that the expression of bcl-2 increased in macrophages treated with MPT64 compared with PPD-treated cells. Furthermore, the results provided strong evidence that bcl-2 up-regulation was positively controlled by miRNA-21. Finally, NF-κB was identified as the transcription factor for miRNA-21 using a ChIP assay. It can be concluded from our study that MPT64 could inhibit the apoptosis of RAW264.7 macrophages through the NF-κB-miRNA21-Bcl-2 pathway.
authors.affiliation: Division of Aviation Medicine, Naval medical Research Institute, Shanghai, China.
authors.forename: Qingmin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Changhai Hospital, the Second Military medical University, Shanghai, China.
authors.forename: Shupeng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Division of Aviation Medicine, Naval medical Research Institute, Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Division of Aviation Medicine, Naval medical Research Institute, Shanghai, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Division of Aviation Medicine, Naval medical Research Institute, Shanghai, China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics; D020868:Gene Silencing; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009163:Mycobacterium bovis / Q000502:physiology; D016328:NF-kappa B / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000942:Antigens, Bacterial; C531950:MIRN21 microRNA, mouse; C510537:MPT64 protein, Mycobacterium tuberculosis; D035683:MicroRNAs; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering