title: Spermidine feeding decreases age-related locomotor activity loss and induces changes in lipid composition.
abstract: Spermidine is a natural polyamine involved in many important cellular functions, whose supplementation in food or water increases life span and stress resistance in several model organisms. In this work, we expand spermidine's range of age-related beneficial effects by demonstrating that it is also able to improve locomotor performance in aged flies. Spermidine's mechanism of action on aging has been primarily related to general protein hypoacetylation that subsequently induces autophagy. Here, we suggest that the molecular targets of spermidine also include lipid metabolism: Spermidine-fed flies contain more triglycerides and show altered fatty acid and phospholipid profiles. We further determine that most of these metabolic changes are regulated through autophagy. Collectively, our data suggests an additional and novel lipid-mediated mechanism of action for spermidine-induced autophagy.
authors.affiliation: Biomedical Sciences Research Complex, University of St. Andrews, St. Andrews, United Kingdom.
authors.forename: Nadège
authors.initials: N
authors.lastname: Minois
authors.affiliation: Institute for Molecular Biosciences, University of Graz, Graz, Austria.
authors.forename: Patrick
authors.initials: P
authors.lastname: Rockenfeller
authors.affiliation: Biomedical Sciences Research Complex, University of St. Andrews, St. Andrews, United Kingdom.
authors.forename: Terry K
authors.initials: TK
authors.lastname: Smith
authors.affiliation: Institute for Molecular Biosciences, University of Graz, Graz, Austria.
authors.forename: Didac
authors.initials: D
authors.lastname: Carmona-Gutierrez
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects*; D010743:Phospholipids / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D013095:Spermidine / Q000008:administration & dosage*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D010743:Phospholipids; D014280:Triglycerides; D006003:Glycogen; D013095:Spermidine