title: Activity of antibacterial, antiviral, anti-inflammatory in compounds andrographolide salt.
abstract: Andrographolide sulfonic acid sodium salt (ASS) was synthesized to increase the the solubility of Andrographolide in aqueous solution. We have studied its pharmacological effect of antibiosis, anti-inflammatory and immunoregulation. Cylinder-plate method was used to study ASS׳s in vitro antibacterial activity, and its protection for mice infected by Staphylococcus aureus and Shigella dysenteriae. Various inflammation models, including the auricular edema induced by xylene in mice, CMC-Na induced air pounch model and the paw edema induced by albumen in rats were used to explore the characteristic of ASS׳s anti-inflammation effect. We built up the immune model by injecting chicken red cells in enter celiac of mice and study the effect of ASS on immunoregulation, taking andrographolide as the positive control. bacteriostasis in vivo and in vitro experiments show that ASS has a weak antibacterial effect and no bactericidal effect, but can reduce the mice mortality of Staphylococcus aureus infected. Anti-inflammatory experiments show that ASS can reduce the mouse ear swelling induced by xylene and rat paw swelling induced by egg albumin, and lessen leukocytes in air bag caused by CMCNa, and lower IL1 not ably in rat serum. Immune tests indicate that ASS can get spleen and thymus gain weight and increase rate of abdominal macrophage phagocytosis of mice. The result of bacteriostasis shows that ASS has weak in vitro antibacterial effect. ASS shows significant effects of anti-inflammation and improving immunity, thus enables the mice against bacteria better.
authors.affiliation: Ministry of Education Key Laboratory of Traditional Chinese Medicine Resources and Compounds, Hubei College of Traditional Chinese Medicine, Hubei, Wuhan 430061, PR China. Electronic address: wenlihu123123@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wen
authors.affiliation: Wuhan Institute of Biological Products Co., Ltd., Hubei, Wuhan 430207, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Xia
authors.affiliation: Wuxi Jimin Kexin Shanhe Pharmaceutical Group Co., Ltd., Jiangsu, Wuxi 430060, PR China.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Traditional Chinese Medicine Resources and Compounds, Hubei College of Traditional Chinese Medicine, Hubei, Wuhan 430061, PR China.
authors.forename: Yingxiu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Ministry of Education Key Laboratory of Traditional Chinese Medicine Resources and Compounds, Hubei College of Traditional Chinese Medicine, Hubei, Wuhan 430061, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Ministry of Education Key Laboratory of Traditional Chinese Medicine Resources and Compounds, Hubei College of Traditional Chinese Medicine, Hubei, Wuhan 430061, PR China.
authors.forename: YaJie
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Traditional Chinese Medicine Resources and Compounds, Hubei College of Traditional Chinese Medicine, Hubei, Wuhan 430061, PR China.
authors.forename: ZiZhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Traditional Chinese Medicine Resources and Compounds, Hubei College of Traditional Chinese Medicine, Hubei, Wuhan 430061, PR China.
authors.forename: YaJie
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000418:Albumins; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D002465:Cell Movement / Q000187:drug effects; D002645:Chickens; D016207:Cytokines / Q000097:blood; D004224:Diterpenes / Q000494:pharmacology* / Q000627:therapeutic use*; D004405:Dysentery, Bacillary / Q000188:drug therapy; D004487:Edema / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy; D004912:Erythrocytes; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D010587:Phagocytosis / Q000187:drug effects; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000188:drug therapy; D014992:Xylenes
chemical_list: D000418:Albumins; D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D000998:Antiviral Agents; D016207:Cytokines; D004224:Diterpenes; D014992:Xylenes; C030419:andrographolide
keywords: Andrographolide sulfonic acid sodium salt; Anti-inflammatory; Antibacterial