title: Eluding anaphylaxis allows peptide-specific prevention of the relapsing stage of experimental autoimmune encephalomyelitis.
abstract: We have used a peptide derived from Acanthamoeba castellanii (ACA) to treat the relapsing phase of EAE that develops in SJL mice following immunization with the PLP 139-151 peptide. The native sequence of the ACA 81-95 peptide that shares key residues with the PLP 139-151 peptide is weakly encephalitogenic in SJL mice but is not recognized by antiserum from SJL mice immunized with PLP 139-151. A single amino acid change to the ACA 81-95 peptide sequence significantly enhanced its encephalitogenicity. When administered to SJL mice as a nonlinear peptide octamer, the modified ACA peptide prevented relapsing episodes of EAE in SJL mice previously immunized with the PLP 139-151 encephalitogenic peptide.
authors.affiliation: Immunology Research Group, United States; Veterans Affairs Medical Center, United States.
authors.forename: Keith W
authors.initials: KW
authors.lastname: Wegmann
authors.affiliation: Immunology Research Group, United States; Veterans Affairs Medical Center, United States.
authors.forename: H G
authors.initials: HG
authors.lastname: Archie Bouwer
authors.affiliation: Veterans Affairs Medical Center, United States; Department of Neurology, Oregon Health & Science University, Portland, OR 97239, United States.
authors.forename: Ruth H
authors.initials: RH
authors.lastname: Whitham
authors.affiliation: Immunology Research Group, United States; Veterans Affairs Medical Center, United States. Electronic address: hinrichs@ohsu.edu.
authors.forename: David J
authors.initials: DJ
authors.lastname: Hinrichs
mesh_terms: D048248:Acanthamoeba castellanii / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D000707:Anaphylaxis / Q000276:immunology* / Q000517:prevention & control*; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology*; D005260:Female; D007108:Immune Tolerance / Q000235:genetics / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D018716:Molecular Mimicry / Q000276:immunology; D008969:Molecular Sequence Data; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000188:drug therapy / Q000276:immunology; D018991:Myelin Proteolipid Protein / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D018991:Myelin Proteolipid Protein; D010446:Peptide Fragments; C435023:myelin proteolipid protein (139-151)
keywords: Peptide mimic; Peptide octamer; Prevention of relapsing disease; Relapsing EAE