title: A new approach to light up the application of semiconductor nanomaterials for photoelectrochemical biosensors: using self-operating photocathode as a highly selective enzyme sensor.
abstract: Due to the intrinsic hole oxidation reaction occurred on the photoanode surface, currently developed photoelectrochemical biosensors suffer from the interference from coexisting reductive species (acting as electron donor) and a novel design strategy of photoelectrode for photoelectrochemical detection is urgently required. In this paper, a self-operating photocathode based on CdS quantum dots sensitized three-dimensional (3D) nanoporous NiO was designed and created, which showed highly selective and reversible response to dissolved oxygen (acting as electron acceptor) in the electrolyte solution. Using glucose oxidase (GOD) as a biocatalyst, a novel photoelectrochemical sensor for glucose was developed. The commonly encountered interferents such as H2O2, ascorbic acid (AA), cysteine (Cys), dopamine (DA), etc., almost had no effect for the cathodic photocurrent of the 3D NiO/CdS electrode, though these substances were proved to greatly influence the photocurrent of photoanodes, which indicated greatly improved selectivity of the method. The method was applied to detect glucose in real samples including serum and glucose injections with satisfactory results. This study could provide a new train of thought on designing of self-operating photocathode in photoelectrochemical sensing, promoting the application of semiconductor nanomaterials in photoelectrochemistry.
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing 210093, PR China. Electronic address: glwang@jiangnan.edu.cn.
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Kang-Li
authors.initials: KL
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Dong
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Xiu-Ming
authors.initials: XM
authors.lastname: Wu
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Zai-Jun
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D019187:Cadmium Compounds / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D004867:Equipment Design; D005949:Glucose Oxidase / Q000378:metabolism; D006801:Humans; D009532:Nickel / Q000737:chemistry; D016062:Porosity; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D018036:Selenium Compounds / Q000737:chemistry; D012666:Semiconductors
chemical_list: D001786:Blood Glucose; D019187:Cadmium Compounds; D004800:Enzymes, Immobilized; D018036:Selenium Compounds; D009532:Nickel; C058667:cadmium selenide; C028007:nickel monoxide; D005949:Glucose Oxidase
keywords: Glucose oxidase; Nanoporous NiO; Photocathode; Photoelectrochemical sensor; Quantum dots