title: Biochemical analysis and antitumour effect of Abrus precatorius agglutinin derived peptides in Ehrlich's ascites and B16 melanoma mice tumour model.
abstract: Anticancer and immunostimulatory properties of tryptic digest peptides of Abrus precatorius agglutinin protein (10kDAGP) have already been reported. Here attempt has been made to further validate anticancer properties of 10kDAGP peptides in Ehrlich's ascites carcinoma (EAC) and B16 melanoma (B16M) bearing mice models and to analyze 10kDAGP by anion exchange chromatography and RP-HPLC for obtaining the bioactive fraction from the total peptide pool. 10kDAGP treatment decreased the tumour pack volume by ∼82% for EAC and 58.8% for B16M. It also showed increase in ex vivo proliferation of splenocyte and thymocyte isolated from tumour bearing mice and increase in TNF-α and Interferon-γ in splenocyte culture supernatant. From chromatographic analysis it was found that anionic peptide fraction may be responsible for anti-proliferative activities of 10kDAGP. As most anticancer peptides are cationic in nature, further studies regarding bioactivity of anionic peptide fraction may lead to novel anticancer peptides and pathways of action.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors.forename: Birendra
authors.initials: B
authors.lastname: Behera
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors.forename: K Sanjana P
authors.initials: KS
authors.lastname: Devi
authors.affiliation: Vellore Institute of Technology, Tamil Nadu 632 014, India.
authors.forename: Debasish
authors.initials: D
authors.lastname: Mishra
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
authors.forename: Swatilekha
authors.initials: S
authors.lastname: Maiti
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal 721302, India. Electronic address: tkmaiti@hijli.iitkgp.ernet.in.
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
mesh_terms: D029912:Abrus; D000373:Agglutinins / Q000737:chemistry; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000373:Agglutinins; D000972:Antineoplastic Agents, Phytogenic; D010455:Peptides
keywords: Abrus precatorius; Agglutinin derived peptides; Anionic anticancer peptides; B16 melanoma; Ehrlich's ascites