title: Cell-microsphere constructs formed with human adipose-derived stem cells and gelatin microspheres promotes stemness, differentiation, and controlled pro-angiogenic potential.
abstract: Translation of stem cell therapies to clinical stage requires viable delivery vehicles that can control stem cell behavior. In this study, the suitability of gelatin microspheres (GMs) as such a tissue engineering platform for human adipose derived stem cells (ADSCs) is investigated. ADSCs on GMs, show a significant upregulation of well known pluripotent marker genes, together with the ability to differentiate into multiple cell lineages of adipocytes, osteocytes, and hepatocytes. In addition, the pro-angiogenic ability of ADSC-GMs is also enhanced compared to ADSCs on 2D culture plates. These results suggest that GMs can be a suitable culture platform for ADSCs with enhanced therapeutic potential.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117585, Singapore.
authors.forename: Anjaneyulu
authors.initials: A
authors.lastname: Kodali
authors.forename: Thiam Chye
authors.initials: TC
authors.lastname: Lim
authors.forename: David Tai
authors.initials: DT
authors.lastname: Leong
authors.forename: Yen Wah
authors.initials: YW
authors.lastname: Tong
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D018914:Cells, Immobilized / Q000166:cytology* / Q000378:metabolism; D018920:Coculture Techniques; D005780:Gelatin / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008863:Microspheres; D018919:Neovascularization, Physiologic; D010011:Osteocytes / Q000166:cytology / Q000378:metabolism; D061251:Primary Cell Culture; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D015415:Biomarkers; D005780:Gelatin
keywords: hepatic differentiation; hydrogel microcarriers; regenerative potential; stem cell therapy; three-dimensional cell culture