title: Telomerase: a target for therapeutic effects of curcumin and a curcumin derivative in Aβ1-42 insult in vitro.
abstract: This study was designed to investigate whether telomerase was involved in the neuroprotective effect of curcumin and Cur1. Alzheimer's disease is a consequence of an imbalance between the generation and clearance of amyloid-beta peptide in the brain. In this study, we used Aβ1-42 (10 µg/ml) to establish a damaged cell model, and curcumin and Cur1 were used in treatment groups. We measured cell survival and cell growth, intracellular oxidative stress and hTERT expression. After RNA interference, the effects of curcumin and Cur1 on cells were verified. Exposure to Aβ1-42 resulted in significant oxidative stress and cell toxicity, and the expression of hTERT was significantly decreased. Curcumin and Cur1 both protected SK-N-SH cells from Aβ1-42 and up-regulated the expression of hTERT. Furthermore, Cur1 demonstrated stronger protective effects than curcumin. However, when telomerase was inhibited by TERT siRNA, the neuroprotection by curcumin and Cur1 were ceased. Our study indicated that the neuroprotective effects of curcumin and Cur1 depend on telomerase, and thus telomerase may be a target for therapeutic effects of curcumin and Cur1.
authors.affiliation: Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Aiwu
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Zhenyang
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Di
authors.affiliation: Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Qi
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yingting
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Guijuan
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Fang
mesh_terms: D000544:Alzheimer Disease / Q000201:enzymology*; D016229:Amyloid beta-Peptides / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006801:Humans; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D010446:Peptide Fragments / Q000633:toxicity; D019098:Telomerase / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; C556037:Cur1 compound; D018696:Neuroprotective Agents; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D019098:Telomerase; D003474:Curcumin