title: Gamma trypsin: purification and physicochemical characterization of a novel bovine trypsin isoform.
abstract: A novel bovine trypsin isoform was purified from commercial sample by ion exchange chromatography by Sephadex SP C50®. New isoform contains in addition of loss of N-terminus hexapeptide (as found in parent molecule β-trypsin) an intra-chain split between Lys-155 and Ser-156. The novel enzyme denominate γ-trypsin showed similar properties with α-trypsin isoform in polypeptide number chain (two chain), molecular masses (23,312 Da), secondary structure, hydrodynamic radius and others. In spite of enzymatic and structural similarities of both isoforms, γ-trypsin preferably has a lower rate formation from β-trypsin, a lower surface charge, but the γ-trypsin has a higher thermal stability than α-trypsin. Due to obtaining facility of purification of bovine trypsin isoforms from commercial font, and properties described above, this enzyme becomes an interesting alternative for the food industry, detergent and biocatalysis research.
authors.affiliation: Pos Graduate Program at Biochemistry and Pharmacology, Federal University of Espírito Santo, Vitória 29040090, ES, Brazil.
authors.forename: Caroline Dutra
authors.initials: CD
authors.lastname: Lacerda
authors.affiliation: Pos Graduate at Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte 31270901, MG, Brazil.
authors.forename: Antônio Ernani
authors.initials: AE
authors.lastname: Teixeira
authors.affiliation: Pos Graduate at Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte 31270901, MG, Brazil.
authors.forename: Jamil Silvano
authors.initials: JS
authors.lastname: de Oliveira
authors.affiliation: Pos Graduate Program at Biochemistry and Pharmacology, Federal University of Espírito Santo, Vitória 29040090, ES, Brazil.
authors.forename: Silvana Fontoura
authors.initials: SF
authors.lastname: Silva
authors.affiliation: Pos Graduate Program at Biochemistry and Pharmacology, Federal University of Espírito Santo, Vitória 29040090, ES, Brazil.
authors.forename: Antônio Victor Baioco
authors.initials: AV
authors.lastname: Vasconcelos
authors.affiliation: Pos Graduate Program at Biochemistry and Pharmacology, Federal University of Espírito Santo, Vitória 29040090, ES, Brazil.
authors.forename: Débora Gonçalves
authors.initials: DG
authors.lastname: Gouveia
authors.affiliation: Federal Institute of Education, Science and Technology, Aracruz, ES 29192733, Brazil.
authors.forename: André Romero
authors.initials: AR
authors.lastname: da Silva
authors.affiliation: Pos Graduate at Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte 31270901, MG, Brazil.
authors.forename: Marcelo Matos
authors.initials: MM
authors.lastname: Santoro
authors.affiliation: Pos Graduate at Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte 31270901, MG, Brazil.
authors.forename: Marcos Luiz
authors.initials: ML
authors.lastname: dos Mares-Guia
authors.affiliation: Pos Graduate Program at Biochemistry and Pharmacology, Federal University of Espírito Santo, Vitória 29040090, ES, Brazil. Electronic address: alexandre.santos@ufes.br.
authors.forename: Alexandre Martins Costa
authors.initials: AM
authors.lastname: Santos
mesh_terms: D000818:Animals; D002417:Cattle; D004789:Enzyme Activation; D004795:Enzyme Stability; D007527:Isoenzymes; D008958:Models, Molecular; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry; D011489:Protein Denaturation; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D013816:Thermodynamics; D014357:Trypsin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D010455:Peptides; D014357:Trypsin
keywords: Enzymatic activity; Physicochemical; Protein stability; Trypsin isoforms; γ-Trypsin