title: Microbial characterization and degradation of linear alkylbenzene sulfonate in an anaerobic reactor treating wastewater containing soap powder.
abstract: The aim of this study was to evaluate the removal of linear alkylbenzene sulfonate (LAS) in an anaerobic fluidized bed reactor (AFBR) treating wastewater containing soap powder as LAS source. At Stage I, the AFBR was fed with a synthetic substrate containing yeast extract and ethanol as carbon sources, and without LAS; at Stage II, soap powder was added to this synthetic substrate obtaining an LAS concentration of 14 ± 3 mg L(-1). The compounds of soap powder probably inhibited some groups of microorganisms, increasing the concentration of volatile fatty acids (VFA) from 91 to 143 mg HAc L(-1). Consequently, the LAS removal rate was 48 ± 10% after the 156 days of operation. By sequencing, 16S rRNA clones belonging to the phyla Proteobacteria and Synergistetes were identified in the samples taken at the end of the experiment, with a remarkable presence of Dechloromonas sp. and Geobacter sp.
authors.affiliation: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. João Dagnone, 1100 - Jd. Santa Angelina, CEP 13563-120 São Carlos, SP, Brazil. Electronic address: mari_carosia@yahoo.com.br.
authors.forename: Mariana Fronja
authors.initials: MF
authors.lastname: Carosia
authors.affiliation: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. João Dagnone, 1100 - Jd. Santa Angelina, CEP 13563-120 São Carlos, SP, Brazil. Electronic address: dagokada@gmail.com.
authors.forename: Dagoberto Yukio
authors.initials: DY
authors.lastname: Okada
authors.affiliation: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. João Dagnone, 1100 - Jd. Santa Angelina, CEP 13563-120 São Carlos, SP, Brazil.
authors.forename: Isabel Kimiko
authors.initials: IK
authors.lastname: Sakamoto
authors.affiliation: Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis, km 235 CEP 13565-905 São Carlos, SP, Brazil. Electronic address: edsilva@ufscar.br.
authors.forename: Edson Luiz
authors.initials: EL
authors.lastname: Silva
authors.affiliation: Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. João Dagnone, 1100 - Jd. Santa Angelina, CEP 13563-120 São Carlos, SP, Brazil. Electronic address: varesche@sc.usp.br.
authors.forename: Maria Bernadete Amâncio
authors.initials: MB
authors.lastname: Varesche
mesh_terms: D017738:Alkanesulfonic Acids / Q000378:metabolism*; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D010802:Phylogeny; D011208:Powders; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012915:Soaps; D013997:Time Factors; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000737:chemistry*; D018508:Water Purification / Q000295:instrumentation* / Q000379:methods*
chemical_list: D017738:Alkanesulfonic Acids; D011208:Powders; D012336:RNA, Ribosomal, 16S; D012915:Soaps; D062065:Wastewater; C030641:alkylbenzyl sulfonic acid
keywords: 16S rRNA; Dechloromonas; Geobacter; Surfactant; Volatile fatty acids