title: Suppressive effects of simvastatin on the human acute promyelocytic leukemia NB4 cell line through the regulation of the nuclear factor-κB signaling pathway.
abstract: The present study examined the effects of simvastatin on the proliferation, apoptosis and gene expression levels involved in the nuclear factor-κB (NF-κB) signaling pathway in the human acute promyelocytic leukemia NB4 cell line by methyl thiazolyl tetrazolium assay, flow cytometry and the Human NF-κB Signaling Pathway RT2Profiler™ PCR Array profiles. The results showed that simvastatin significantly inhibited proliferation and induced apoptosis of the NB4 cells in a time- and dose-dependent manner. Changes were noted in the expression levels of 56 genes involved in the NF-κB signaling pathways in the NB4 cells treated with 15 μm simvastatin at 48 h post-incubation, among which, 47 genes were downregulated and 9 were upregulated. In conclusion, simvastatin potentially inhibits the proliferation and induces the apoptosis of NB4 cells through the regulation of the expression levels of genes involved in the NF-κB signaling pathway.
authors.affiliation: Laboratory of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Qiu
authors.affiliation: Department of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Xie
authors.affiliation: Laboratory of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Hematology, The First People's Hospital of Kunshan, Kunshan, Jiangsu 215300, P.R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zeng
authors.affiliation: Department of Hematology, Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi 545005, P.R. China.
authors.forename: Tingxiu
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Zhilan
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Laboratory of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Dai
authors.affiliation: Department of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Hua
authors.affiliation: Department of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Weiying
authors.initials: W
authors.lastname: Gu
keywords: NB4 cell; NF-κB signaling pathway; PCR array; apoptosis; proliferation; simvastatin