title: Catalytic strategy used by the myosin motor to hydrolyze ATP.
abstract: Myosin is a molecular motor responsible for biological motions such as muscle contraction and intracellular cargo transport, for which it hydrolyzes adenosine 5'-triphosphate (ATP). Early steps of the mechanism by which myosin catalyzes ATP hydrolysis have been investigated, but still missing are the structure of the final ADP·inorganic phosphate (Pi) product and the complete pathway leading to it. Here, a comprehensive description of the catalytic strategy of myosin is formulated, based on combined quantum-classical molecular mechanics calculations. A full exploration of catalytic pathways was performed and a final product structure was found that is consistent with all experiments. Molecular movies of the relevant pathways show the different reorganizations of the H-bond network that lead to the final product, whose γ-phosphate is not in the previously reported HPγO4(2-) state, but in the H2PγO4(-) state. The simulations reveal that the catalytic strategy of myosin employs a three-pronged tactic: (i) Stabilization of the γ-phosphate of ATP in a dissociated metaphosphate (PγO3(-)) state. (ii) Polarization of the attacking water molecule, to abstract a proton from that water. (iii) Formation of multiple proton wires in the active site, for efficient transfer of the abstracted proton to various product precursors. The specific role played in this strategy by each of the three loops enclosing ATP is identified unambiguously. It explains how the precise timing of the ATPase activation during the force generating cycle is achieved in myosin. The catalytic strategy described here for myosin is likely to be very similar in most nucleotide hydrolyzing enzymes.
authors.affiliation: Computational Biochemistry, Interdisciplinary Center for Scientific Computing, University of Heidelberg, D-69120 Heidelberg, Germany.
authors.forename: Farooq Ahmad
authors.initials: FA
authors.lastname: Kiani
authors.affiliation: Computational Biochemistry, Interdisciplinary Center for Scientific Computing, University of Heidelberg, D-69120 Heidelberg, Germany stefan.fischer@iwr.uni-heidelberg.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Fischer
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D055162:Biocatalysis; D018360:Crystallography, X-Ray; D006868:Hydrolysis; D008958:Models, Molecular; D009218:Myosins / Q000737:chemistry / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D000255:Adenosine Triphosphate; D009218:Myosins