title: Angelica sinensis induces hair regrowth via the inhibition of apoptosis signaling.
abstract: Hair loss is accompanied by keratinocyte apoptosis-regression during catagen and prolonged telogen. Angelica sinensis was reported to promote hair growth in vitro. Based on previous studies, we explored the hair growth effect and the mechanism of A. sinensis related to keratinocyte apoptosis-regression during catagen in mice. The 70% Ethanol extract of A. sinensis was applied topically at doses of 1 and 100 mg/mL to the dorsa of C57BL/6 mice for 2 weeks. The A. sinensis-treated group showed noticeable hair regrowth. Treatment with A. sinensis restored the lengths of hair shafts and size of hair follicles. In addition, mice treated with A. sinensis showed notably decreased apoptotic cells, along with a significant change in the expression of cleaved caspase-3 and the ratio of a pair of apoptosis-associated proteins: Bcl-2 and Bax. Also, A. sinensis inhibited the nuclear translocation of NF-κB, the phosphorylation of IκB-α, the phosphorylation of three mitogen-activated protein MAP kinases, and the activation of c-Jun with decreased TNF-α. These findings reveal a role of A. sinensis as an alternative treatment for hair loss that acts through hair cycle pathways associated with apoptosis regression during catagen.
authors.affiliation: College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Mi Hye
authors.initials: MH
authors.lastname: Kim
authors.forename: You Yeon
authors.initials: YY
authors.lastname: Choi
authors.forename: Ik-Hyun
authors.initials: IH
authors.lastname: Cho
authors.forename: Jongki
authors.initials: J
authors.lastname: Hong
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Woong Mo
authors.initials: WM
authors.lastname: Yang
mesh_terms: D000287:Administration, Topical; D029971:Angelica sinensis; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D053148:Caspase 3 / Q000378:metabolism; D005260:Female; D006197:Hair / Q000254:growth & development*; D018859:Hair Follicle / Q000187:drug effects* / Q000254:growth & development*; D020672:I-kappa B Proteins / Q000378:metabolism; D015603:Keratinocytes / Q000473:pathology*; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494827:Bax protein, mouse; D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D016755:Proto-Oncogene Proteins c-jun; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein; D000072000:NF-KappaB Inhibitor alpha; D020928:Mitogen-Activated Protein Kinases; D053148:Caspase 3
keywords: Angelica sinensis (Oliv.) Diels; Apoptosis; Hair Follicle; Hair Loss