title: Evaluation of the association between blood homocysteine concentration and the degree of behavioral symptoms in the 6-hydroxydopamine-induced Parkinsonism in rat.
abstract: Growing evidence indicates that homocysteine (Hcy) may be involved in the pathophysiology of several neurological disorders including Parkinson's disease. In the present study, the association between blood Hcy concentration and the degree of behavioral symptoms in the 6-hydroxydopamine (6-OHDA)-induced Parkinsonism in rat was evaluated. Total serum Hcy (tHcy) was measured before and 6 weeks after the intracerebral injection of 6-OHDA. Apomorphine-induced rotational test was performed at second, third and sixth weeks after 6-OHDA injection. Subsequently, cell replacement therapy was performed on rats with good rotation score. No correlation between tHcy in before 6-OHDA injection and severity of the rotations after 6-OHDA injection was observed. On the other hand, 6-OHDA treatment significantly decreased tHcy level. However, this reduction was only observed in animals with low degree of rotations and in rats with high number of rotations; tHcy did not change significantly. Furthermore, 10 weeks after cell transplantation, tHcy was significantly lower than that found before therapy if the rats showed good improvement in the degree of rotations. We also examined the effect of different supplements of B vitamins on tHcy before and after 6-OHDA injection. In healthy rats, all kinds of B vitamins and also supplement B6 or B12 alone reduced tHcy. Following 6-OHDA injection, B vitamin supplementation failed to cause remarkable effect. Considering the direct correlation between the severity of rotational behavior and the degree of lesion in the substantia nigra (SN), our data indicate that higher tHcy values can predict higher SN dopaminergic neurodegeneration.
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Electronic address: hhaghdoost@yahoo.com.
authors.forename: Hashem
authors.initials: H
authors.lastname: Haghdoost-Yazdi
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Sarookhani
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Ayda
authors.initials: A
authors.lastname: Faraj
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Negin
authors.initials: N
authors.lastname: Fraidouni
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Dargahi
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Mohammad Hosein
authors.initials: MH
authors.lastname: Yaghoubidoust
authors.affiliation: Department of Basic sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Azhdari-Zarmehri
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D017690:Cell Transplantation; D004195:Disease Models, Animal; D006710:Homocysteine / Q000097:blood*; D008297:Male; D016627:Oxidopamine / Q000633:toxicity*; D010300:Parkinson Disease / Q000097:blood / Q000523:psychology* / Q000628:therapy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006710:Homocysteine; D016627:Oxidopamine
keywords: 6-Hydroxydopamine; Apomorphine-induced rotational test; B vitamin supplementation; Cell replacement therapy; Homocysteine; Parkinson's disease