title: Antidepressant-like activity of adhyperforin, a novel constituent of Hypericum perforatum L.
abstract: Adhyperforin is a novel constituent of Hypericum perforatum L., but its antidepressant-like activity remains unclear. To explore that, several well-validated animal models of depression as well as neurotransmitter reuptake and transporter binding assays were conducted. The results showed adhyperforin could reduce the immobility time of mice in the forced swimming test and tail suspension assay, antagonize the behaviors induced by reserpine, and have no effect on locomotor activity. Furthermore, following establishment of a chronic unpredictable mild stress model, adhyperforin increased the number of crossings and rearings in rats in the open field test and increased the sucrose consumption. Finally, adhyperforin inhibited uptake of serotonin, norepinephrine, and dopamine, and displayed robust binding affinities for the serotonin and norepinephrine transporters. Overall, the current study provides the first evidence that adhyperforin is a novel, active ingredient of Hypericum perforatum L. with robust antidepressant-like activity.
authors.affiliation: 1] Key Laboratory of Molecular Pharmacology and Drug Evaluation (Ministry of Education of China), School of Pharmacy, Yantai University, Yantai 264005, China [2].
authors.forename: Jingwei
authors.initials: J
authors.lastname: Tian
authors.affiliation: 1] Key Laboratory of Molecular Pharmacology and Drug Evaluation (Ministry of Education of China), School of Pharmacy, Yantai University, Yantai 264005, China [2].
authors.forename: Fangxi
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Pharmacology and Drug Evaluation (Ministry of Education of China), School of Pharmacy, Yantai University, Yantai 264005, China.
authors.forename: Jucan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Beijing Peking University WBL Biotech Co., Ltd., Beijing, 100094, PR China.
authors.forename: Shuren
authors.initials: S
authors.lastname: Guo
authors.affiliation: Beijing Peking University WBL Biotech Co., Ltd., Beijing, 100094, PR China.
authors.forename: Pinglan
authors.initials: P
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Pharmacology and Drug Evaluation (Ministry of Education of China), School of Pharmacy, Yantai University, Yantai 264005, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D003863:Depression / Q000188:drug therapy*; D004298:Dopamine / Q000378:metabolism; D019417:Hindlimb Suspension / Q000379:methods; D020902:Hypericum / Q000737:chemistry*; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D009638:Norepinephrine / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012110:Reserpine / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism; D013550:Swimming / Q000502:physiology; D013729:Terpenes / Q000494:pharmacology*
chemical_list: D000928:Antidepressive Agents; D010936:Plant Extracts; D013729:Terpenes; C423123:adhyperforin; D012701:Serotonin; D012110:Reserpine; D004298:Dopamine; D009638:Norepinephrine