title: Ultra-performance liquid chromatography-mass spectrometry as a sensitive and powerful technology in lipidomic applications.
abstract: Lipidomics, the comprehensive illumination of lipid-based information in biology systems, involves in identifying lipids and profiling lipids and lipid-derived mediators. The development of lipidomics enables the characterization of lipid species and detailed lipid profiling in body fluid, tissue or cell, and allows for a wider understanding of the biological roles of lipid networks. Lipidomic research has been greatly facilitated by recent advances in ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) and involved in lipid extraction, lipid identification and data analysis supporting applications from qualitative and quantitative assessment of multiple lipid species. UPLC technique, different mass spectrometry technique, lipid extraction and data analysis in lipidomics are reviewed. Afterwards, examples are provided on the use of UPLC-MS for finding lipid biomarkers in disease, drug, food, nutrition and plant fields. We also discuss the UPLC-MS-based lipidomics for the future perspectives and their potential problems.
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, The College of Life Sciences, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, PR China; Division of Nephrology and Hypertension, School of Medicine, University of California, Irvine, MedSci 1, C352, UCI Campus, Irvine, CA 92868, USA. Electronic address: zyy@nwu.edu.cn.
authors.forename: Ying-Yong
authors.initials: YY
authors.lastname: Zhao
authors.affiliation: Sorbonne Universités, UPMC Univ. Paris 06, CNRS UMR 8232, IPCM, 4 place Jussieu, 75005 Paris, France.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Wu
authors.affiliation: Division of Nephrology and Hypertension, School of Medicine, University of California, Irvine, MedSci 1, C352, UCI Campus, Irvine, CA 92868, USA.
authors.forename: Shuman
authors.initials: S
authors.lastname: Liu
authors.affiliation: Sorbonne Universités, UPMC Univ. Paris 06, CNRS UMR 8232, IPCM, 4 place Jussieu, 75005 Paris, France.
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Beijing 100029, PR China. Electronic address: linrch307@sina.com.
authors.forename: Rui-Chao
authors.initials: RC
authors.lastname: Lin
mesh_terms: D015415:Biomarkers / Q000737:chemistry; D002853:Chromatography, Liquid; D019295:Computational Biology / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D008055:Lipids / Q000032:analysis / Q000737:chemistry*; D013058:Mass Spectrometry; D010944:Plants / Q000737:chemistry
chemical_list: D015415:Biomarkers; D008055:Lipids
keywords: Lipid biomarker; Lipid profiling; Lipidomics; Mass spectrometry; Ultra-performance liquid chromatography