title: Rational design of a ligand-based antagonist of jasmonate perception.
abstract: (+)-7-iso-Jasmonoyl-L-isoleucine (JA-Ile) regulates developmental and stress responses in plants. Its perception involves the formation of a ternary complex with the F-box COI1 and a member of the JAZ family of co-repressors and leads to JAZ degradation. Coronatine (COR) is a bacterial phytotoxin that functionally mimics JA-Ile and interacts with the COI1-JAZ co-receptor with higher affinity than JA-Ile. On the basis of the co-receptor structure, we designed ligand derivatives that spatially impede the interaction of the co-receptor proteins and, therefore, should act as competitive antagonists. One derivative, coronatine-O-methyloxime (COR-MO), has strong activity in preventing the COI1-JAZ interaction, JAZ degradation and the effects of JA-Ile or COR on several JA-mediated responses in Arabidopsis thaliana. Moreover, it potentiates plant resistance, preventing the effect of bacterially produced COR during Pseudomonas syringae infections in different plant species. In addition to the utility of COR-MO for plant biology research, our results underscore its biotechnological potential for safer and sustainable agriculture.
authors.affiliation: Plant Molecular Genetics Department, National Centre for Biotechnology (CNB), Consejo Superior de Investigaciones Científicas (CSIC), Campus University Autónoma, Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Monte
authors.affiliation: Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Hamberg
authors.affiliation: Plant Molecular Genetics Department, National Centre for Biotechnology (CNB), Consejo Superior de Investigaciones Científicas (CSIC), Campus University Autónoma, Madrid, Spain.
authors.forename: Andrea
authors.initials: A
authors.lastname: Chini
authors.affiliation: Plant Molecular Genetics Department, National Centre for Biotechnology (CNB), Consejo Superior de Investigaciones Científicas (CSIC), Campus University Autónoma, Madrid, Spain.
authors.forename: Selena
authors.initials: S
authors.lastname: Gimenez-Ibanez
authors.affiliation: Genomics Unit, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, Madrid, Spain.
authors.forename: Gloria
authors.initials: G
authors.lastname: García-Casado
authors.affiliation: Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Halle, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Porzel
authors.affiliation: Systems Biology Department, CNB, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, Madrid, Spain.
authors.forename: Florencio
authors.initials: F
authors.lastname: Pazos
authors.affiliation: Plant Molecular Genetics Department, National Centre for Biotechnology (CNB), Consejo Superior de Investigaciones Científicas (CSIC), Campus University Autónoma, Madrid, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Boter
authors.affiliation: Plant Molecular Genetics Department, National Centre for Biotechnology (CNB), Consejo Superior de Investigaciones Científicas (CSIC), Campus University Autónoma, Madrid, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Solano
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D021542:Amino Acids, Neutral / Q000494:pharmacology*; D000872:Anthocyanins / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D020171:Botrytis / Q000472:pathogenicity; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D015195:Drug Design; D018506:Gene Expression Regulation, Plant; D007192:Indenes / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D007532:Isoleucine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D008024:Ligands; D010091:Oximes / Q000494:pharmacology*; D054883:Oxylipins / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000472:pathogenicity; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000596:Amino Acids; D021542:Amino Acids, Neutral; D000872:Anthocyanins; D029681:Arabidopsis Proteins; C092776:CONSTANS protein, Arabidopsis; D003517:Cyclopentanes; D004268:DNA-Binding Proteins; D007192:Indenes; C000592055:JAZ9 protein, Arabidopsis; D008024:Ligands; D010091:Oximes; D054883:Oxylipins; D012097:Repressor Proteins; D014157:Transcription Factors; C000592054:coronatine-O-methyloxime; C532883:jasmonoyl-isoleucine; D007532:Isoleucine; C058487:coronatine; C011006:jasmonic acid