title: Association of human leukocyte antigen DQB1 and DRB1 alleles with chronic hepatitis B.
abstract: OBJECTIVE
  To investigate the effect of human leukocyte antigen (HLA) DRB1 and DQB1 alleles on the inactive and advanced stages of chronic hepatitis B.
  
  
  METHODS
  Patient records at a single institution's hepatology clinic were reviewed. Demographic data, laboratory results, endoscopy results, virological parameters, biopsy scores and treatment statuses were recorded. In total, 355 patients were eligible for the study, of whom 226 (63.7%) were male. Overall, 82 (23.1%) were hepatitis B early antigen (HBeAg) positive, 87 (24.5%) had cirrhosis, and 66 (18.6%) had inactive disease. The presence of DQB1 and DRB1 alleles was determined by polymerase chain reaction with sequence-specific primers. The distribution of the genotyped alleles among patients with cirrhosis and patients with chronic active hepatitis was analyzed.
  
  
  RESULTS
  The most frequent HLA DQB1 allele was DQB1*03:01 (48.2%), and the most frequent HLA DRB1 allele was DRB1*13/14 (51.8%). DQB1*05:01 was more frequent in patients with active disease than in inactive patients (27% vs 9.1%; P = 0.002, Pc = 0.026). DRB1*07 was rare in patients with cirrhosis compared with non-cirrhotics (3.4% vs 16%; P = 0.002, Pc = 0.022). Older age (P < 0.001) and male gender (P = 0.008) were the other factors that affected the presence of cirrhosis. In a multivariate logistic regression analysis, DRB1*07 remained a significant negative predictor of cirrhosis (P = 0.015). A bioinformatics analysis revealed that a polymorphic amino acid sequence in DRB1*07 may alter interaction with the T-cell recognition site.
  
  
  CONCLUSIONS
  This study demonstrates that HLA alleles may influence cirrhosis development and disease activity in Turkish chronic hepatitis B patients.
authors.affiliation: Levent Doganay, Feruze Yilmaz Enc, Yasar Colak, Celal Ulasoglu, Ilyas Tuncer, Department of Gastroenterology, Goztepe Teaching and Research Hospital, Medeniyet University, Istanbul 34722, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Doganay
authors.affiliation: Levent Doganay, Feruze Yilmaz Enc, Yasar Colak, Celal Ulasoglu, Ilyas Tuncer, Department of Gastroenterology, Goztepe Teaching and Research Hospital, Medeniyet University, Istanbul 34722, Turkey.
authors.forename: Arta
authors.initials: A
authors.lastname: Fejzullahu
authors.affiliation: Levent Doganay, Feruze Yilmaz Enc, Yasar Colak, Celal Ulasoglu, Ilyas Tuncer, Department of Gastroenterology, Goztepe Teaching and Research Hospital, Medeniyet University, Istanbul 34722, Turkey.
authors.forename: Seyma
authors.initials: S
authors.lastname: Katrinli
authors.affiliation: Levent Doganay, Feruze Yilmaz Enc, Yasar Colak, Celal Ulasoglu, Ilyas Tuncer, Department of Gastroenterology, Goztepe Teaching and Research Hospital, Medeniyet University, Istanbul 34722, Turkey.
authors.forename: Feruze
authors.initials: F
authors.lastname: Yilmaz Enc
authors.affiliation: Levent Doganay, Feruze Yilmaz Enc, Yasar Colak, Celal Ulasoglu, Ilyas Tuncer, Department of Gastroenterology, Goztepe Teaching and Research Hospital, Medeniyet University, Istanbul 34722, Turkey.
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Ozturk
authors.affiliation: Levent Doganay, Feruze Yilmaz Enc, Yasar Colak, Celal Ulasoglu, Ilyas Tuncer, Department of Gastroenterology, Goztepe Teaching and Research Hospital, Medeniyet University, Istanbul 34722, Turkey.
authors.forename: Yasar
authors.initials: Y
authors.lastname: Colak
authors.affiliation: Levent Doganay, Feruze Yilmaz Enc, Yasar Colak, Celal Ulasoglu, Ilyas Tuncer, Department of Gastroenterology, Goztepe Teaching and Research Hospital, Medeniyet University, Istanbul 34722, Turkey.
authors.forename: Celal
authors.initials: C
authors.lastname: Ulasoglu
authors.affiliation: Levent Doganay, Feruze Yilmaz Enc, Yasar Colak, Celal Ulasoglu, Ilyas Tuncer, Department of Gastroenterology, Goztepe Teaching and Research Hospital, Medeniyet University, Istanbul 34722, Turkey.
authors.forename: Ilyas
authors.initials: I
authors.lastname: Tuncer
authors.affiliation: Levent Doganay, Feruze Yilmaz Enc, Yasar Colak, Celal Ulasoglu, Ilyas Tuncer, Department of Gastroenterology, Goztepe Teaching and Research Hospital, Medeniyet University, Istanbul 34722, Turkey.
authors.forename: Gizem
authors.initials: G
authors.lastname: Dinler Doganay
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D016009:Chi-Square Distribution; D019295:Computational Biology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D059811:HLA-DRB1 Chains / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology / Q000821:virology; D006801:Humans; D008103:Liver Cirrhosis / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology / Q000821:virology; D016015:Logistic Models; D008297:Male; D008969:Molecular Sequence Data; D015999:Multivariate Analysis; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011379:Prognosis; D065840:Protective Factors; D012189:Retrospective Studies; D012307:Risk Factors; D012737:Sex Factors; D014421:Turkey
chemical_list: D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D059811:HLA-DRB1 Chains
keywords: Chronic active hepatitis; Cirrhosis; Hepatitis B; Human leukocyte antigen DQ; Human leukocyte antigen DR