title: Facile synthesis of magnetic mesoporous hollow carbon microspheres for rapid capture of low-concentration peptides.
abstract: Mesoporous and hollow carbon microspheres embedded with magnetic nanoparticles (denoted as MHM) were prepared via a facile self-sacrificial method for rapid capture of low-abundant peptides from complex biological samples. The morphology, structure, surface property, and magnetism were well-characterized. The hollow magnetic carbon microspheres have a saturation magnetization value of 130.2 emu g(-1) at room temperature and a Brunauer-Emmett-Teller specific surface area of 48.8 m(2) g(-1) with an average pore size of 9.2 nm for the mesoporous carbon shell. The effectiveness of these MHM affinity microspheres for capture of low-concentration peptides was evaluated by standard peptides, complex protein digests, and real biological samples. These multifunctional hollow carbon microspheres can realize rapid capture and convenient separation of low-concentration peptides. They were validated to have better performance than magnetic mesoporous silica and commercial peptide-enrichment products. In addition, they can be easily recycled and present excellent reusability. Therefore, it is expected that this work may provide a promising tool for high-throughput discovery of peptide biomarkers from biological samples for disease diagnosis and other biomedical applications.
authors.affiliation: Department of Biomedical Engineering, The Pennsylvania State University , University Park, Pennsylvania 16802, United States.
authors.forename: Gong
authors.initials: G
authors.lastname: Cheng
authors.forename: Ming-Da
authors.initials: MD
authors.lastname: Zhou
authors.forename: Si-Yang
authors.initials: SY
authors.lastname: Zheng
mesh_terms: D000327:Adsorption; D000804:Angiotensin II / Q000302:isolation & purification; D000818:Animals; D002244:Carbon / Q000737:chemistry*; D002417:Cattle; D006736:Horses; D006801:Humans; D060328:Magnetic Phenomena; D008280:Magnetics / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009584:Nitrogen / Q000737:chemistry; D010455:Peptides / Q000302:isolation & purification*; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013696:Temperature; D014556:Urine; D014961:X-Ray Diffraction
chemical_list: D010455:Peptides; D000804:Angiotensin II; D012710:Serum Albumin, Bovine; D002244:Carbon; D009584:Nitrogen