title: Structure of Yin Yang 1 oligomers that cooperate with RuvBL1-RuvBL2 ATPases.
abstract: Yin Yang 1 (YY1) is a transcription factor regulating proliferation and differentiation and is involved in cancer development. Oligomers of recombinant YY1 have been observed before, but their structure and DNA binding properties are not well understood. Here we find that YY1 assembles several homo-oligomeric species built from the association of a bell-shaped dimer, a process we characterized by electron microscopy. Moreover, we find that YY1 self-association also occurs in vivo using bimolecular fluorescence complementation. Unexpectedly, these oligomers recognize several DNA substrates without the consensus sequence for YY1 in vitro, and DNA binding is enhanced in the presence of RuvBL1-RuvBL2, two essential AAA+ ATPases. YY1 oligomers bind RuvBL1-RuvBL2 hetero-oligomeric complexes, but YY1 interacts preferentially with RuvBL1. Collectively, these findings suggest that YY1-RuvBL1-RuvBL2 complexes could contribute to functions beyond transcription, and we show that YY1 and the ATPase activity of RuvBL2 are required for RAD51 foci formation during homologous recombination.
authors.affiliation: Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maetzu 9, 28040 Madrid, Spain.
authors.forename: Andrés
authors.initials: A
authors.lastname: López-Perrote
authors.affiliation: Genome Damage and Stability Centre, University of Sussex, Science Park Road, Falmer, Brighton BN1 9RQ, United Kingdom, and.
authors.forename: Hanan E
authors.initials: HE
authors.lastname: Alatwi
authors.affiliation: Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maetzu 9, 28040 Madrid, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Torreira
authors.affiliation: Genome Damage and Stability Centre, University of Sussex, Science Park Road, Falmer, Brighton BN1 9RQ, United Kingdom, and.
authors.forename: Amani
authors.initials: A
authors.lastname: Ismail
authors.affiliation: Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Darwin 3, 28049 Madrid, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Ayora
authors.affiliation: Genome Damage and Stability Centre, University of Sussex, Science Park Road, Falmer, Brighton BN1 9RQ, United Kingdom, and. Electronic address: J.A.Downs@sussex.ac.uk.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Downs
authors.affiliation: Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maetzu 9, 28040 Madrid, Spain,. Electronic address: ollorca@cib.csic.es.
authors.forename: Oscar
authors.initials: O
authors.lastname: Llorca
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D059765:Homologous Recombination / Q000502:physiology; D006801:Humans; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding / Q000502:physiology; D055503:Protein Multimerization / Q000502:physiology*; D051135:Rad51 Recombinase / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology; D050986:YY1 Transcription Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D046912:Multiprotein Complexes; D050986:YY1 Transcription Factor; C494698:YY1 protein, human; D004247:DNA; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; D000074183:ATPases Associated with Diverse Cellular Activities; D004265:DNA Helicases; C486549:RUVBL1 protein, human; C122079:RUVBL2 protein, human
keywords: ATPase; DNA Repair; Electron Microscopy (EM); RuvBL1; RuvBL2; Single Particle Analysis; Structural Biology; Transcription Factor; YY1