title: Effect of oral consumption of probiotic Lactobacillus planatarum P-8 on fecal microbiota, SIgA, SCFAs, and TBAs of adults of different ages.
abstract: OBJECTIVE
  Probiotics may improve intestinal health by modulating intestinal microbiota. However, the age-related variation in response to probiotic consumption is understudied. The aim of this study was to determine the effect of oral consumption of Lactobacillus plantarum P-8 (Lp-8) on human intestinal microflora, secretary immunoglobulin A (SIgA), total bile acids (TBAs), and short-chain fatty acids (SCFAs) of different aged adults.
  
  
  METHODS
  Thirty-three recruited individuals, including young (mean age 26 y), middle-aged (mean age 51 y), and elderly (mean age 76 y) volunteers, were given a single daily oral dose of Lp-8 (6 × 10(10) colony forming units) for 4 wk. Fecal samples were collected before starting, during, and after stopping Lp-8 intake. Polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis, quantitative PCR, high-performance liquid chromatography, and enzyme-linked immunosorbent assay were used to detect for fecal microflora, SIgA, TBAs, and SCFAs.
  
  
  RESULTS
  Results showed an increase in Bifidobacterium (P < 0.05) and other beneficial bacteria, whereas Desulfovibrio (P < 0.05) and other opportunistic pathogens decreased after taking Lp-8 for 4 wk. Lp-8 consumption also affected fecal levels of SIgA, TBAs, and SCFAs.
  
  
  CONCLUSIONS
  Lp-8 administration could improve human gastrointestinal health. However, some of these effects were transient and gradually disappeared once the intervention was discontinued. Moreover, the extent of these desirable effects was age-related.
authors.affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, P. R. China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, P. R. China.
authors.forename: Jiachao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, P. R. China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, P. R. China.
authors.forename: Laiyu
authors.initials: L
authors.lastname: Kwok
authors.affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, P. R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Ma
authors.affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, P. R. China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, P. R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, P. R. China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Huang
authors.affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, P. R. China. Electronic address: Hepingdd@vip.sina.com.
authors.forename: Heping
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001644:Bifidobacterium / Q000254:growth & development; D001647:Bile Acids and Salts / Q000378:metabolism*; D003106:Colon / Q000378:metabolism / Q000382:microbiology*; D003901:Desulfovibrio / Q000254:growth & development; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D007778:Lactobacillus; D064307:Microbiota; D008875:Middle Aged; D019936:Probiotics
chemical_list: D001647:Bile Acids and Salts; D005232:Fatty Acids, Volatile; D007070:Immunoglobulin A
keywords: Fecal microbiota; Gut microbiota; Human; Lactobacillus plantarum; Lp-8; Probiotics