title: Microbial transglutaminase treatment in pasta-production does not affect the immunoreactivity of gliadin with celiac disease patients' sera.
abstract: The effect of microbial transglutaminase (MTG)-treatment of pasta-dough on the immunoreactivity with celiac disease patient's sera has been investigated. Modification by MTG has been proven by determination of the MTG reaction product ε-(γ-glutamyl)lysine (3.63 μmol/g protein), which was not detectable in non-MTG-treated pasta. Antigenicity has been analyzed by immunoblotting and ELISA using gliadin-extracts from pasta and MTG-treated pasta. Immunoblotting showed that the antibody-population (antigliadin antibodies and antideamidated gliadin antibodies) of the sera is specific for every individual patient. Immunoblotting and ELISA showed that there is no difference in immunoreactivity of gliadin extracted from pasta and MTG-pasta. Recognition pattern and intensity in Western blot as well as antibody titer has also been identical even for sera with a high antideamidated gliadin antibody titer. These results indicate no difference between pasta-gliadin and MTG-pasta-gliadin and especially no increased deamidation in pasta-gliadin by MTG-treatment.
authors.affiliation: Zedira GmbH , Roesslerstr. 83, 64293 Darmstadt, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Ruh
authors.forename: Jürgen
authors.initials: J
authors.lastname: Ohsam
authors.forename: Ralf
authors.initials: R
authors.lastname: Pasternack
authors.forename: Keiichi
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kumazawa
authors.forename: Martin
authors.initials: M
authors.lastname: Hils
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000917:Antibody Formation / Q000187:drug effects*; D002446:Celiac Disease / Q000097:blood / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004151:Dipeptides / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005511:Food Handling / Q000379:methods*; D005903:Gliadin / Q000032:analysis / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007223:Infant; D008875:Middle Aged; D013302:Streptomyces / Q000201:enzymology; D011503:Transglutaminases / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry; D055815:Young Adult
chemical_list: D004151:Dipeptides; C001299:epsilon-(gamma-glutamyl)-lysine; D005903:Gliadin; D011503:Transglutaminases