title: Tissue and cellular localization of tannins in Tunisian dates (Phoenix dactylifera L.) by light and transmission electron microscopy.
abstract: A histological approach including light microscopy and transmission electron microscopy (TEM) was used to provide accurate information on the localization of condensed tannins in the edible tissues and in the stone of date fruits (Phoenix dactylifera L.). Light microscopy was carried out on fresh tissues after staining by 4-dimethylaminocinnamaldehyde (DMACA) for a specific detection of condensed tannins. Thus, whether under light microscopy or transmission electron microscopy (TEM), results showed that tannins are not located in the epidermis but more deeply in the mesocarp in the vacuole of very large cells. Regarding the stones, tannins are found in a specific cell layer located at 50 μm from the sclereid cells of the testa.
authors.affiliation: Laboratoire d'Application de la Chimie aux Ressources et Substances Naturelles et à l'Environnement (LACReSNE), , Faculté des Sciences de Bizerte , 7021 Zarzouna-Bizerte, Tunisie.
authors.forename: Hédi
authors.initials: H
authors.lastname: Hammouda
authors.forename: Camille
authors.initials: C
authors.lastname: Alvarado
authors.forename: Brigitte
authors.initials: B
authors.lastname: Bouchet
authors.forename: Jamila
authors.initials: J
authors.lastname: Kalthoum-Chérif
authors.forename: Malika
authors.initials: M
authors.lastname: Trabelsi-Ayadi
authors.forename: Sylvain
authors.initials: S
authors.lastname: Guyot
mesh_terms: D005638:Fruit / Q000737:chemistry* / Q000648:ultrastructure; D008854:Microscopy, Electron; D046529:Microscopy, Electron, Transmission; D065749:Phoeniceae / Q000737:chemistry*; D044945:Proanthocyanidins / Q000032:analysis; D012639:Seeds / Q000737:chemistry / Q000648:ultrastructure; D013634:Tannins / Q000032:analysis*; D014416:Tunisia
chemical_list: D044945:Proanthocyanidins; D013634:Tannins