title: MicroRNA-205 regulates the calcification and osteoblastic differentiation of vascular smooth muscle cells.
abstract: OBJECTIVE
  We investigated the role of miR-205 in the osteogenic differentiation of vascular smooth muscle cells (VSMCs).
  
  
  METHODS
  Osteogenic differentiation of human aortic smooth muscle cells (HASMCs) was induced by 10 mM β-glycerophosphate (β-GP). Alizarin Red S staining, alkaline phosphatase (ALP) activity and osteocalcin secretion were used to determine osteogenic differentiation of HASMCs. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expression of miR-205 in HASMCs.
  
  
  RESULTS
  The expression of endogenous miR-205 was decreased in HASMCs during β-glycerophosphate-induced calcification. Overexpression of miR-205 inhibited the differentiation of HASMCs into osteoblast-like cells, as evidenced by a decrease in ALP activity, osteocalcin secretion, and Runx2 expression, whereas miR-205 depletion enhanced osteoblastic differentiation of HASMCs. Runx2 and Smad1 were identified as direct targets of miR-205 by computational analysis and experimental assays.
  
  
  CONCLUSIONS
  The present study shows that miR-205 may negatively regulate the β-glycerophosphate-induced calcification of HASMCs, at least partially by targeting Runx2 and Smad1.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Qiao
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D015153:Blotting, Western; D002113:Calcification, Physiologic / Q000235:genetics*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005994:Glycerophosphates / Q000494:pharmacology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051898:Smad1 Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000469:Alkaline Phosphatase; D050659:Core Binding Factor Alpha 1 Subunit; D005994:Glycerophosphates; D035683:MicroRNAs; D015675:Osteocalcin; D051898:Smad1 Protein; C534425:MIR205, human; C493709:RUNX2 protein, human; C497399:SMAD1 protein, human; C031463:beta-glycerophosphoric acid