title: Differential gene-expression profiles from canine cumulus cells of ovulated versus in vitro-matured oocytes.
abstract: We compared the nuclear maturation status and gene-expression profiles of canine cumulus cells (CCs) derived from cumulus-oocyte complexes (COCs) that were spontaneously ovulated versus those that were matured in vitro. Cumulus-oocyte complexes were retrieved from uteri by surgical flushing (after spontaneous ovulation) or by ovariectomy follicle aspiration and in vitro maturation. The objective of Experiment 1 was to investigate the nuclear maturation status of in vivo- versus in vitro-matured oocytes. The objective of Experiment 2 was to compare gene-expression profiles of CCs derived from in vivo- versus in vitro-matured COCs. Genes analysed are related to cell maturation, development and apoptosis, including GDF9, MAPK1, PTX3, CX43, Bcl2 and BAX; mRNA expression for all of these genes, except for GDF9, differed (P<0.05) between in vivo- and in vitro-matured CCs. In conclusion, we found that gene-expression profiles are related to the quality of CCs and therefore posit that monitoring gene expression could be a useful strategy to guide attempts to improve in vitro culture systems.
authors.affiliation: Department of Animal Science, Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Gyeongsangnam-Do, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Cho
authors.affiliation: Department of Animal Science, Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Gyeongsangnam-Do, Republic of Korea.
authors.forename: Kyeong-Lim
authors.initials: KL
authors.lastname: Lee
authors.affiliation: Department of Animal Science, Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Gyeongsangnam-Do, Republic of Korea.
authors.forename: Yu-Gon
authors.initials: YG
authors.lastname: Kim
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Suwon 441-706, Gyeonggi-Do, Republic of Korea.
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Dairy Science Division, National Institute of Animal Science, Cheonan 331-801, Chungcheongnam-Do, Republic of Korea.
authors.forename: Jae-Gyu
authors.initials: JG
authors.lastname: Yoo
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Suwon 441-706, Gyeonggi-Do, Republic of Korea.
authors.forename: Byoung-Chul
authors.initials: BC
authors.lastname: Yang
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Suwon 441-706, Gyeonggi-Do, Republic of Korea.
authors.forename: Jin-Ki
authors.initials: JK
authors.lastname: Park
authors.affiliation: Department of Animal Science, Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Gyeongsangnam-Do, Republic of Korea.
authors.forename: Il-Keun
authors.initials: IK
authors.lastname: Kong
mesh_terms: D000818:Animals; D054885:Cumulus Cells / Q000378:metabolism*; D004285:Dogs; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D059471:In Vitro Oocyte Maturation Techniques; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis; D010060:Ovulation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger