title: Metallization of double-stranded DNA triggered by bound galactose-modified naphthalene diimide.
abstract: Naphthalene diimide (NDI) derivatives bearing galactose moieties through different spacers, NDI-DS1 and NDI-DS2, were synthesized by the click reaction of the acetylene derivatives of NDI with galactose azide. They bound to double-stranded DNA with threading intercalation, as confirmed by the topoisomerase I assay and circular dichroism spectroscopy. The binding affinities of these ligands were on the order of 10(5) M(-1) with several-fold higher affinity for double-stranded DNA than for single-stranded DNA. The silver mirror reaction on the double-stranded DNA bound to these ligands afforded silver nanowires that were converted to gold nanowires. In the atomic force microscopy measurements, the increased height of DNA areas on a mica plate was observed in the case of double-stranded DNA after NDI-DS2 treatment and subsequently silver mirror reaction, whereas the increased height of DNA areas was not observed in the case of single-stranded DNA after the same treatment.
authors.affiliation: Department of Applied Chemistry and ‡Research Center for Biomicrosensing Technology, Kyushu Institute of Technology , 1-1 Sensui-cho, Tobata-ku, Kitakyushu, Fukuoka 804-8550, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Komizo
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ikedo
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sato
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D057930:Click Chemistry; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D005690:Galactose / Q000737:chemistry*; D007094:Imides / Q000737:chemistry*; D008958:Models, Molecular; D009281:Naphthalenes / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012834:Silver / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D004277:DNA, Single-Stranded; D007094:Imides; D009281:Naphthalenes; C542131:naphthalenediimide; D012834:Silver; D004247:DNA; D005690:Galactose