title: [Construction of plant expression vectors with PMI gene as selection marker and their utilization in transformation of Salvia miltiorrhiza f. alba].
abstract: OBJECTIVE
  To construct plant expression pCAMBIA1301-PMI by substituting PMI for hygromycin resistance gene in pCAMBIA1301 and obtain transgenic Salvia miltiorrhiza f. alba using PMI-mannose selection system.
  
  
  METHODS
  The 6-phosphomannose isomerase gene (PMI) of Escherichia coli was amplified by PCR. Sequence analysis showed that it shared 100% amino acids identities with the sequences of PMI genes isolates reported in the NCBI. Based on pCAMBIA1305, the plant expression pCAMBIA1305-PMI was constructed successfully by substituting PMI for hygromycin resistance gene in pCAMBIA1305. pCAMBIA1305-PMI was transformed into Agrobacterium tumefaciens LBA4404, and then the leaves of S. miltiorrhiza f. alba were inoculated in LBA4404 with pCAMBIA1305-PMI.
  
  
  RESULTS
  Plant expression pCAMBIA1301-PMI was successfully constructed and the leaves of S. miltiorrhiza f. alba inoculated in LBA4404 with pCAMBIA1305-PMI were selected on medium supplemented with a combination of 20 g x L(-1) mannose and 10 g x L(-1) sucrose as a carbon source. The transformation efficiency rate was 23.7%.
  
  
  CONCLUSIONS
  Genetic transformation was confirmed by PCR, indicating that a new method for obtaining transgenic S. miltiorrhiza f. alba plants was developed using PMI-mannose selection system.
authors.forename: Ru
authors.initials: R
authors.lastname: Tao
authors.forename: You-Can
authors.initials: YC
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Fang
authors.forename: Ren-Jiu
authors.initials: RJ
authors.lastname: Shi
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Li
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Gang-Ping
authors.initials: GP
authors.lastname: Hao
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015415:Biomarkers; D002934:Cinnamates / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D008359:Mannose-6-Phosphate Isomerase / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D015415:Biomarkers; D002934:Cinnamates; D029968:Escherichia coli Proteins; D006921:Hygromycin B; C026273:hygromycin A; D008359:Mannose-6-Phosphate Isomerase