title: Evaluation of autofluorescence and toluidine blue in the differentiation of oral dysplastic and neoplastic lesions from non dysplastic and neoplastic lesions: a cross-sectional study.
abstract: The objective was to compare toluidine blue (TB) and autofluorescence (AF) for the detection of oral dysplasia and squamous cell carcinoma (OSCC) in clinically suspicious lesions according to conventional examination. Fifty-six clinically suspicious lesions were subjected to AF and TB examination. Data were compared using two different scenarios: in the first, mild dysplasia was considered as positive, while in the second, it was considered as negative. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV), accuracy, and concordance were calculated. AF sensitivity and specificity were 70.0 and 57.7%, respectively, while TB showed a sensitivity of 80% and a specificity of 61.5%. The sensitivity increased in the second scenario in both AF (76.5%) and TB (88.2%). The specificity decreased in AF and TB, showing the same value (51.3%). PPV was higher in TB than in AF (70.6 versus 65.6%) and similarly for NPV (72.7 versus 62.5%). In the second scenario, TB PPV was 44.1% and NPV was 90.9%; AF PPV was 40.6% and NPV was 83.3%. TB showed greater accuracy than AF in the first scenario (62.5 versus 58.9%). AF and TB are both sensitive but not specific in OSCC and dysplasia diagnosis.
authors.affiliation: University of Bari "Aldo Moro," Dipartimento Interdisciplinare di Medicina, Sezione Malattie Odontostomatologiche-Policlinico di Bari, Piazza Giulio Cesare, 11- 70124 Bari, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Petruzzi
authors.affiliation: Second University of Naples, Multidisciplinary Department of Medical-Surgical and Odontostomatological Specialties; Via L. De Crecchio, 6 80138 Naples, Italy.
authors.forename: Alberta
authors.initials: A
authors.lastname: Lucchese
authors.affiliation: University "La Sapienza" of Rome, Department of Oral and Maxillo Facial Sciences, Via Caserta, Rome, Italy.
authors.forename: Gianna Maria
authors.initials: GM
authors.lastname: Nardi
authors.affiliation: University of Milano Bicocca, Department of Neuroscience and Biomedical Technologies, Via Cadore, 48, Monza, Italy.
authors.forename: Dorina
authors.initials: D
authors.lastname: Lauritano
authors.affiliation: University of Bari "Aldo Moro," Dipartimento Interdisciplinare di Medicina, Sezione Malattie Odontostomatologiche-Policlinico di Bari, Piazza Giulio Cesare, 11- 70124 Bari, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Favia
authors.affiliation: Second University of Naples, Multidisciplinary Department of Medical-Surgical and Odontostomatological Specialties; Via L. De Crecchio, 6 80138 Naples, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Serpico
authors.affiliation: University of Bari "Aldo Moro," Dipartimento Interdisciplinare di Medicina, Sezione Malattie Odontostomatologiche-Policlinico di Bari, Piazza Giulio Cesare, 11- 70124 Bari, Italy.
authors.forename: Felice Roberto
authors.initials: FR
authors.lastname: Grassi
mesh_terms: D004396:Coloring Agents; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000737:chemistry / Q000473:pathology; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000473:pathology; D061848:Optical Imaging / Q000379:methods*; D011237:Predictive Value of Tests; D014048:Tolonium Chloride
chemical_list: D004396:Coloring Agents; D014048:Tolonium Chloride