title: Labrenzia sp. BM1: a quorum quenching bacterium that degrades N-acyl homoserine lactones via lactonase activity.
abstract: We report the degradation of quorum sensing N-acylhomoserine lactone molecules by a bacterium isolated from a Malaysian marine water sample. MALDI-TOF and phylogenetic analysis indicated this isolate BM1 clustered closely to Labrenzia sp. The quorum quenching activity of this isolate was confirmed by using a series of bioassays and rapid resolution liquid chromatography analysis. Labrenzia sp. degraded a wide range of N-acylhomoserine lactones namely N-(3-hexanoyl)-L-homoserine lactone (C6-HSL), N-(3-oxohexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-L-homoserine lactone (3-hydroxy-C6-HSL). Re-lactonisation bioassays confirmed Labrenzia sp. BM1 degraded these signalling molecules efficiently via lactonase activity. To the best of our knowledge, this is the first documentation of a Labrenzia sp. capable of degrading N-acylhomoserine lactones and confirmation of its lactonase-based mechanism of action.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. norshazliza_shaz@ymail.com.
authors.forename: Norshazliza Ab
authors.initials: NA
authors.lastname: Ghani
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. outfeet@yahoo.com.
authors.forename: Siti Nur Maisarah
authors.initials: SN
authors.lastname: Norizan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. xinyuechan@gmail.com.
authors.forename: Xin Yue
authors.initials: XY
authors.lastname: Chan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. yinwaifong@yahoo.com.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. kokgan@um.edu.my.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D020561:Alphaproteobacteria / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D004789:Enzyme Activation; D053038:Quorum Sensing / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D013045:Species Specificity
chemical_list: D054742:Acyl-Butyrolactones; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase