title: The biological effects of sex hormones on rabbit articular chondrocytes from different genders.
abstract: The aim of this study was to investigate the biological effects of sex hormones (17β-estradiol and testosterone) on rabbit articular chondrocytes from different genders. We cultured primary rabbit articular chondrocytes from both genders with varying concentration of sex hormones. We evaluate cell proliferation and biochemical functions by MTT and GAG assay. The chondrocyte function and phenotypes were analyzed by mRNA level using RT-PCR. Immunocytochemical staining was also used to evaluate the generation of collagen-II. This study demonstrated that 17β-estradiol had greater positive regulation on the biological function and gene expressions of articular chondrocytes than testosterone, with the optimal concentrations of 10(-6) and 10(-7) M, particularly for female chondrocytes.
authors.affiliation: Department of Biomedical Engineering, I-Shou University, Kaohsiung 81346, Taiwan.
authors.forename: Shwu Jen
authors.identifier: 0000-0001-9434-7221
authors.initials: SJ
authors.lastname: Chang
authors.affiliation: Department of Biomedical Engineering, I-Shou University, Kaohsiung 81346, Taiwan.
authors.forename: Shyh Ming
authors.identifier: 0000-0003-0706-398X
authors.initials: SM
authors.lastname: Kuo
authors.affiliation: Department of Biomedical Engineering, I-Shou University, Kaohsiung 81346, Taiwan.
authors.forename: Yen Ting
authors.initials: YT
authors.lastname: Lin
authors.affiliation: Department of Orthopedics, Kaohsiung Veterans General Hospital, 386 Ta-Chung 1st Road, Kaohsiung 81300, Taiwan.
authors.forename: Shan-Wei
authors.identifier: 0000-0002-9924-0703
authors.initials: SW
authors.lastname: Yang
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000008:administration & dosage*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D061251:Primary Cell Culture; D011817:Rabbits; D012727:Sex Characteristics; D013739:Testosterone / Q000008:administration & dosage*
chemical_list: D013739:Testosterone; D004958:Estradiol