title: A protocol to infect Caenorhabditis elegans with Salmonella typhimurium.
abstract: In the last decade, C. elegans has emerged as an invertebrate organism to study interactions between hosts and pathogens, including the host defense against gram-negative bacterium Salmonella typhimurium. Salmonella establishes persistent infection in the intestine of C. elegans and results in early death of infected animals. A number of immunity mechanisms have been identified in C. elegans to defend against Salmonella infections. Autophagy, an evolutionarily conserved lysosomal degradation pathway, has been shown to limit the Salmonella replication in C. elegans and in mammals. Here, a protocol is described to infect C. elegans with Salmonella typhimurium, in which the worms are exposed to Salmonella for a limited time, similar to Salmonella infection in humans. Salmonella infection significantly shortens the lifespan of C. elegans. Using the essential autophagy gene bec-1 as an example, we combined this infection method with C. elegans RNAi feeding approach and showed this protocol can be used to examine the function of C. elegans host genes in defense against Salmonella infection. Since C. elegans whole genome RNAi libraries are available, this protocol makes it possible to comprehensively screen for C. elegans genes that protect against Salmonella and other intestinal pathogens using genome-wide RNAi libraries.
authors.affiliation: Department of Biological Sciences, Florida Atlantic University.
authors.forename: Jiuli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Biological Sciences, Florida Atlantic University; kjia@fau.edu.
authors.forename: Kailiang
authors.initials: K
authors.lastname: Jia
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000382:microbiology*; D004195:Disease Models, Animal; D054884:Host-Pathogen Interactions / Q000235:genetics; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D012481:Salmonella Infections, Animal / Q000235:genetics* / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000502:physiology*
chemical_list: D012330:RNA, Double-Stranded