title: Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina.
abstract: Different species of Mycoplasma can affect bovine cattle, causing several diseases. PCR sequencing and further analysis of the 16S-23S rRNA ITS region have shown a significant interspecies variability among Mollicutes. Sixteen suspected isolates of Mycoplasma spp. obtained from milk samples from dairy herds were amplified (16S-23S rRNA ITS region). Fourteen out of those 16 suspected Mycoplasma spp. isolates were PCR-positive. To confirm the identity of Mycoplasma bovis, these 14 isolates were tested by another species-specific PCR. Seven of the isolates rendered a positive result. The products of 16S-23S rRNA ITS PCR from one isolate that was identified as M. bovis and from two other isolates, identified as non- M. bovis were randomly selected, sequenced and analyzed. The three sequences (A, B and C) showed 100% similarity with M. bovis, Mycoplasma canadense and Mycoplasma californicum respectively.
authors.affiliation: Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, República Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), República Argentina. Electronic address: topo.vet@gmail.com.
authors.forename: Pablo J
authors.initials: PJ
authors.lastname: Tamiozzo
authors.affiliation: Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, República Argentina.
authors.forename: Abel A
authors.initials: AA
authors.lastname: Estanguet
authors.affiliation: Laboratorio Lactodiagnóstico Sur Sociedad Responsabilidad Limitada (SRL), Olivos, Buenos Aires, República Argentina.
authors.forename: Julia
authors.initials: J
authors.lastname: Maito
authors.affiliation: Laboratorio Lactodiagnóstico Sur Sociedad Responsabilidad Limitada (SRL), Olivos, Buenos Aires, República Argentina.
authors.forename: Liliana
authors.initials: L
authors.lastname: Tirante
authors.affiliation: Laboratorio Lactodiagnóstico Sur Sociedad Responsabilidad Limitada (SRL), Olivos, Buenos Aires, República Argentina.
authors.forename: Martin
authors.initials: M
authors.lastname: Pol
authors.affiliation: Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, República Argentina.
authors.forename: José A
authors.initials: JA
authors.lastname: Giraudo
mesh_terms: D000818:Animals; D001118:Argentina; D002417:Cattle / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D009174:Mycoplasma / Q000302:isolation & purification*
keywords: 16S-23S ARNr; 16S-23S rRNA; Dairy cattle; Ganado lechero; ITS; Mycoplasma bovis; Mycoplasma californicum; Mycoplasma canadense