title: Eosinophil purification from peripheral blood.
abstract: Eosinophils are granulocytes integral to allergic inflammation and parasitic responses and comprise 1-4 % of the circulating leukocytes in human beings under normal conditions. Isolation of human eosinophils allows for ex vivo and in vitro experimentation, providing a valuable tool for the study of allergic mechanisms. Here, we describe a technique for the isolation of human eosinophils by negative selection from whole blood obtained by venipuncture.
authors.affiliation: Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, KSB 23, 330 Brookline Avenue, Boston, 02215, MA, USA, pakuthot@bidmc.harvard.edu.
authors.forename: Praveen
authors.initials: P
authors.lastname: Akuthota
authors.forename: Kelsey
authors.initials: K
authors.lastname: Capron
authors.forename: Peter F
authors.initials: PF
authors.lastname: Weller
mesh_terms: D002469:Cell Separation / Q000379:methods*; D004804:Eosinophils / Q000166:cytology*; D006801:Humans; D018962:Phlebotomy