title: Determination of mechanical properties of spatially heterogeneous breast tissue specimens using contact mode atomic force microscopy (AFM).
abstract: This paper outlines a comprehensive parametric approach for quantifying mechanical properties of spatially heterogeneous thin biological specimens such as human breast tissue using contact-mode Atomic Force Microscopy. Using inverse finite element (FE) analysis of spherical nanoindentation, the force response from hyperelastic material models is compared with the predicted force response from existing analytical contact models, and a sensitivity study is carried out to assess uniqueness of the inverse FE solution. Furthermore, an automation strategy is proposed to analyze AFM force curves with varying levels of material nonlinearity with minimal user intervention. Implementation of our approach on an elastic map acquired from raster AFM indentation of breast tissue specimens indicates that a judicious combination of analytical and numerical techniques allow more accurate interpretation of AFM indentation data compared to relying on purely analytical contact models, while keeping the computational cost associated an inverse FE solution with reasonable limits. The results reported in this study have several implications in performing unsupervised data analysis on AFM indentation measurements on a wide variety of heterogeneous biomaterials.
authors.forename: Rajarshi
authors.initials: R
authors.lastname: Roy
authors.forename: Jaydev P
authors.initials: JP
authors.lastname: Desai
mesh_terms: D000465:Algorithms; D057205:Automation, Laboratory; D001940:Breast / Q000502:physiology*; D005260:Female; D020342:Finite Element Analysis; D006801:Humans; D055595:Mechanical Phenomena; D018625:Microscopy, Atomic Force / Q000379:methods*; D008962:Models, Theoretical; D046888:Tissue Array Analysis