title: Effect of the inhibitors phenylalanine arginyl ß-naphthylamide (PAßN) and 1-(1-naphthylmethyl)-piperazine (NMP) on expression of genes in multidrug efflux systems of Escherichia coli isolates from bovine mastitis.
abstract: The multidrug efflux system in bacteria can reduce antibiotic concentration inside the cell, leading to failure in the treatment of bacterial diseases. This study evaluated the influence of two efflux pump inhibitors (EPIs), phenylalanine arginyl ß-naphthylamide (PAßN) and 1-(1-naphthylmethyl)-piperazine (NMP), on the gene expression of three multidrug efflux systems, AcrAB, AcrEF and EmrAB in Escherichia coli bovine mastitis isolates resistant to ampicillin and sulfamethoxazole/trimethoprim simultaneously. Each isolate had at least three multidrug efflux system genes. The acrA and acrB had the lowest expression levels in all treatments, while the emrA or emrB showed the highest expression levels in the presence of ampicillin, sulfamethoxazole/trimethoprim, PAβN and NMP. EPIs also contributed to the decrease in arcF expression when used in combination with ampicillin treatment. Since PAßN showed stronger effects than NMP, it may serve as an alternative to assist in the antimicrobial therapy of mastitis.
authors.affiliation: Laboratório de Doenças Bacterianas, Setor de Medicina Veterinária Preventiva e Saúde Pública (SMVPSP), Departamento de Veterinária (DVT), Universidade Federal de Viçosa (UFV) Campus Universitário, 36570-000, Viçosa, Minas Gerais, Brazil.
authors.forename: M A Ospina
authors.initials: MA
authors.lastname: Barrero
authors.affiliation: Laboratório de Doenças Bacterianas, Setor de Medicina Veterinária Preventiva e Saúde Pública (SMVPSP), Departamento de Veterinária (DVT), Universidade Federal de Viçosa (UFV) Campus Universitário, 36570-000, Viçosa, Minas Gerais, Brazil.
authors.forename: P A G
authors.initials: PA
authors.lastname: Pietralonga
authors.affiliation: Laboratório de Doenças Bacterianas, Setor de Medicina Veterinária Preventiva e Saúde Pública (SMVPSP), Departamento de Veterinária (DVT), Universidade Federal de Viçosa (UFV) Campus Universitário, 36570-000, Viçosa, Minas Gerais, Brazil.
authors.forename: D G G
authors.initials: DG
authors.lastname: Schwarz
authors.affiliation: Laboratório de Virologia Animal, SMVPSP, DVT, UFV, Viçosa, Minas Gerais, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Silva
authors.affiliation: Laboratório de Imunovirologia Molecular, Departamento de Biologia Geral, UFV, Viçosa, Minas Gerais, Brazil.
authors.forename: S O
authors.initials: SO
authors.lastname: Paula
authors.affiliation: Laboratório de Doenças Bacterianas, Setor de Medicina Veterinária Preventiva e Saúde Pública (SMVPSP), Departamento de Veterinária (DVT), Universidade Federal de Viçosa (UFV) Campus Universitário, 36570-000, Viçosa, Minas Gerais, Brazil. Electronic address: masm@ufv.br.
authors.forename: M A S
authors.initials: MA
authors.lastname: Moreira
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000235:genetics; D002417:Cattle; D015169:Colony Count, Microbial; D004151:Dipeptides / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D008414:Mastitis, Bovine / Q000188:drug therapy / Q000382:microbiology*; D026901:Membrane Transport Proteins / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000662:veterinary; D010879:Piperazines / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology
chemical_list: C511391:1-(1-naphthylmethyl)piperazine; D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D004151:Dipeptides; D026901:Membrane Transport Proteins; D010879:Piperazines; C419365:phenylalanine arginine beta-naphthylamide; D000667:Ampicillin; D015662:Trimethoprim, Sulfamethoxazole Drug Combination
keywords: Antimicrobial; Milk; Multiresistant bacteria; Therapy