title: Phytochrome A antagonizes PHYTOCHROME INTERACTING FACTOR 1 to prevent over-activation of photomorphogenesis.
abstract: Phytochrome A (phyA) is crucial to initiate the early steps of the transition between skoto- and photomorphogenesis upon light exposure and to complete this process under far-red light (typical of dense vegetation canopies). However, under prolonged red or white light, phyA mutants are hyper-photomorphogenic in many respects. To investigate this issue, we analyzed the late response of the transcriptome of the phyA mutant to red light. Compared to the wild-type (WT), hyper-responsive genes outnumbered the genes showing reduced response to red light in phyA. A network analysis revealed the co-expression of PHYTOCHROME INTERACTING FACTOR 1 (PIF1) with those genes showing hyper-promotion by red light in phyA. The enhanced responses of gene expression, cotyledon unfolding, hypocotyl growth, and greening observed in the phyA mutant compared to the WT were absent in the phyA pif1 double mutant compared to pif1, indicating that the hyper-photomorphogenic phenotype of phyA requires PIF1. PIF1 directly binds to gene promoters that displayed PIF1-mediated enhanced response to red light. Expression of mutant PIF1 deficient in interactions with phyA and phyB enhanced the long-term growth response to red light but reduced the expression of selected genes in response to red light. We propose that phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis during early seedling development.
authors.affiliation: IFEVA, Faculty of Agronomy, University of Buenos Aires, and Consejo Nacional de Investigaciones Científicas y Técnicas, Av. San Martín 4453, 1417-Buenos Aires, Argentina.
authors.forename: Martín
authors.initials: M
authors.lastname: Krzymuski
authors.affiliation: Fundación Instituto Leloir, IIBBA-CONICET, C1405BWE-Buenos Aires, Argentina.
authors.forename: Pablo D
authors.initials: PD
authors.lastname: Cerdán
authors.affiliation: Department of Molecular Biosciences and the Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Molecular Biosciences and the Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Vinh
authors.affiliation: Plant Biology Laboratory, Howard Hughes Medical Institute, the Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
authors.forename: Joanne
authors.initials: J
authors.lastname: Chory
authors.affiliation: Department of Molecular Biosciences and the Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA.
authors.forename: Enamul
authors.initials: E
authors.lastname: Huq
authors.affiliation: IFEVA, Faculty of Agronomy, University of Buenos Aires, and Consejo Nacional de Investigaciones Científicas y Técnicas, Av. San Martín 4453, 1417-Buenos Aires, Argentina; Fundación Instituto Leloir, IIBBA-CONICET, C1405BWE-Buenos Aires, Argentina. Electronic address: casal@ifeva.edu.ar.
authors.forename: Jorge J
authors.initials: JJ
authors.lastname: Casal
mesh_terms: D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000737:chemistry / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D008027:Light; D009154:Mutation; D051942:Phytochrome A / Q000235:genetics / Q000378:metabolism*; D051939:Phytochrome B / Q000378:metabolism; D055550:Protein Stability / Q000528:radiation effects; D017434:Protein Structure, Tertiary; D059748:Proteolysis / Q000528:radiation effects; D036226:Seedlings / Q000254:growth & development / Q000528:radiation effects
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D051942:Phytochrome A; D051939:Phytochrome B; C508068:At2g20180 protein, Arabidopsis
keywords: PIF1; de-etiolation; light (gene expression); light (growth).; phyA