title: Thermal and pH Sensitive Multifunctional Polymer Nanoparticles for Cancer Imaging and Therapy.
abstract: In this study, we prepared novel poly(Glycerol malate co-dodecanedioate) (PGMD) NPs containing an imaging/hyperthermia agent (IR820) and a chemotherapeutic agent (doxorubicin, DOX). The PGMD polymer was prepared by thermal condensation. IR820 and DOX loaded PGMD NPs were prepared using the single oil emulsion technique. The size of the NPs measured was around 150 nm. Drug loading efficiency of DOX and IR820 was around 4% and 8%, respectively. An acidic environment (pH=5.0) induced higher DOX release as compared to pH=7.4. DOX release was also enhanced by exposure to laser, which increased the temperature to 42°C. Cytotoxicity of the drug loaded NPs was comparable in MES-SA but was higher in Dx5 cells compared to free drug (p<0.05). The combination of hyperthermia and chemotherapy improved cytotoxicity in both cell lines. The NP formulation significantly improved the plasma half-life of IR820 in mice after tail vein injection.
authors.affiliation: Biomedical Engineering Department, Florida International University, 10555 West Flagler Street, Miami, FL 33174, USA ; Cirle, 1951 NW 7 Ave, Suite 13106, Miami, FL, 33136.
authors.forename: Tingjun
authors.initials: T
authors.lastname: Lei
authors.affiliation: Biomedical Engineering Department, Florida International University, 10555 West Flagler Street, Miami, FL 33174, USA ; Department of Basic and Applied Sciences. Galgotias University, UP, 201308, India.
authors.forename: Romila
authors.initials: R
authors.lastname: Manchanda
authors.affiliation: Biomedical Engineering Department, Florida International University, 10555 West Flagler Street, Miami, FL 33174, USA ; Physical Therapy Department, Nova Southeastern University, 3200 S. University Dr., Fort Lauderdale, FL 33328, USA.
authors.forename: Alicia
authors.initials: A
authors.lastname: Fernandez-Fernandez
authors.affiliation: Biomedical Engineering Department, Florida International University, 10555 West Flagler Street, Miami, FL 33174, USA.
authors.forename: Yen-Chih
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Biomedical Engineering Department, Florida International University, 10555 West Flagler Street, Miami, FL 33174, USA.
authors.forename: Douglas
authors.initials: D
authors.lastname: Wright
authors.affiliation: Biomedical Engineering Department, Florida International University, 10555 West Flagler Street, Miami, FL 33174, USA.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: McGoron
keywords: IR820; chemotherapy; hyperthermia; multifunctional nanoparticles; poly(Glycerol malate co-dodecanedioate) (PGMD)