title: Blood born miRNAs signatures that can serve as disease specific biomarkers are not significantly affected by overall fitness and exercise.
abstract: Blood born micro(mi)RNA expression pattern have been reported for various human diseases with signatures specific for diseases. To evaluate these biomarkers, it is mandatory to know possible changes of miRNA signatures in healthy individuals under different physiological conditions. We analyzed the miRNA expression in peripheral blood of elite endurance athletes and moderatly active controls. Blood drawing was done before and after exhaustive exercise in each group. After Benjamini-Hochberg adjustment we did not find any miRNA with significant p-values when comparing miRNA expression between the different groups. We found, however, 24 different miRNAs with an expression fold change of minimum 1.5 in at least one of the comparisons (athletes before vs after exercise, athletes before exercise vs controls and athletes after exercise vs controls). The observed changes are not significant in contrast to the expression changes of the blood born miRNA expression reported for many human diseases. These data support the idea of disease associated miRNA patterns useful as biomarkers that are not readily altered by physiological conditions.
authors.affiliation: Department of Human Genetics, Medical School, Saarland University, Homburg/Saar, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Backes
authors.affiliation: Department of Human Genetics, Medical School, Saarland University, Homburg/Saar, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Leidinger
authors.affiliation: Department of Clinical Bioinformatics, Saarland University, Saarbruecken, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Keller
authors.affiliation: Department of Human Genetics, Medical School, Saarland University, Homburg/Saar, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hart
authors.affiliation: Institute of Sports and Preventive Medicine, Saarland University, Saarbruecken, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Meyer
authors.affiliation: Department of Human Genetics, Medical School, Saarland University, Homburg/Saar, Germany.
authors.forename: Eckart
authors.initials: E
authors.lastname: Meese
authors.affiliation: Institute of Sports and Preventive Medicine, Saarland University, Saarbruecken, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Hecksteden
mesh_terms: D000328:Adult; D056352:Athletes; D014408:Biomarkers, Tumor / Q000097:blood*; D015444:Exercise; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000097:blood*; D009369:Neoplasms / Q000097:blood / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs