title: Measurement of eosinophil kinetics in healthy volunteers.
abstract: Radiolabelled leukocyte scans are widely used in nuclear medicine to locate sites of infection and inflammation. Radiolabelling of leukocyte subpopulations can also yield valuable information on cell trafficking and kinetics in vivo, but care must be taken to minimize inadvertent cell activation ex vivo. Here, we describe the use of autologous indium(111)-labelled eosinophils to measure eosinophil intravascular life-span and monitor their distribution and fate using gamma camera imaging in healthy non-atopic individuals.
authors.affiliation: Department of Medicine, Addenbrooke's and Papworth Hospitals, University of Cambridge School of Clinical Medicine, Cambridge, CB2 0QQ, UK, nf231@cam.ac.uk.
authors.forename: Neda
authors.initials: N
authors.lastname: Farahi
authors.forename: Chrystalla
authors.initials: C
authors.lastname: Loutsios
authors.forename: Rosalind P
authors.initials: RP
authors.lastname: Simmonds
authors.forename: Linsey
authors.initials: L
authors.lastname: Porter
authors.forename: Daniel
authors.initials: D
authors.lastname: Gillett
authors.forename: Sarah
authors.initials: S
authors.lastname: Heard
authors.forename: A Michael
authors.initials: AM
authors.lastname: Peters
authors.forename: Alison M
authors.initials: AM
authors.lastname: Condliffe
authors.forename: Edwin R
authors.initials: ER
authors.lastname: Chilvers
mesh_terms: D004804:Eosinophils / Q000166:cytology* / Q000378:metabolism*; D064368:Healthy Volunteers; D006801:Humans; D007205:Indium Radioisotopes; D007700:Kinetics
chemical_list: D007205:Indium Radioisotopes