title: Effect of Isoproterenol on LDL Susceptibility to Oxidation and Serum Total Antioxidant Capacity in Cyclosporine-Treated Rats.
abstract: BACKGROUND
  Cyclosporine therapy is associated with a variety of adverse effects. Recent studies have suggested increased oxidative stress as a cause of these side effects.
  
  
  OBJECTIVE
  Since, melatonin is one of the most powerful known antioxidants, and considering that isoproterenol is one of the drugs stimulating endogenous melatonin production, we tried to determine the effect of isoproterenol on LDL susceptibility to oxidation and serum total antioxidant capacity in cyclosporine-treated rats.
  
  
  METHODS
  32 male Wistar rats were divided into four groups: group A were controls that received placebo; group B received intraperitoneal isoproterenol (20 mg/kg/d) alone; group C received intravenous cyclosporine (15 mg/kg/d) alone; and group D received both drugs simultaneously at the same doses and durations-cyclosporine one week after administration of isoproterenol. Blood samples were drawn four times from rats in each group: before injections, during the treatment, end of the treatment, and one week after the last injections.
  
  
  RESULTS
  There was a significant (p<0.05) increase in LDL susceptibility to oxidation, and a decrease in serum total antioxidant capacity (p<0.05) in group C rats. But, there were no significant changes in group B and D rats in terms of LDL susceptibility to oxidation and total antioxidant capacity.
  
  
  CONCLUSIONS
  Isoproterenol may be capable of delaying adverse effects of cyclosporine by preventing the increase in LDL susceptibility to oxidation, and decrease in serum total antioxidant capacity.
authors.affiliation: I.A.U. Tabriz Branch, Young Researchers Club, Tabriz, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Foroughimoghaddam
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Ghorbanihaghjo
authors.affiliation: Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Rashtchizadeh
authors.affiliation: Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Argani
keywords: Cyclosporine; Isoproterenol; LDL Susceptibility to Oxidation; Rat; Total Antioxidant