title: Surface-modified silk hydrogel containing hydroxyapatite nanoparticle with hyaluronic acid-dopamine conjugate.
abstract: Silk fibroin/hydroxyapatite (SF/HAp) composite hydrogels were fabricated in this study, having different HAp contents (0-33 wt%) in SF matrix hydrogel. Surface modification of HAp nanoparticle with hyaluronic acid (HA)-dopamine (DA) conjugate improved a dispersibility of HAp in aqueous SF solution due to its negatively charged surface and therefore, fabrication of the SF composite hydrogel having HAp nanoparticles inside could be possible. Zeta potential of surface-modified HAP was examined by ELS. It demonstrates that surface of HAp was well modified to a negative charge with HA-DA. Morphological structure of SF hydrogel containing surface-modified HAp was examined by FE-SEM for analyzing pore structure of hydrogel and deposition of HAp nanoparticle in SF hydrogel. It was found that HAp nanoparticles were uniformly deposited on the pore wall of SF hydrogel. Structural characteristics of SF/HAp composite hydrogel was performed using X-ray diffraction and FT-IR analysis. It was found that β-sheet crystal conformation of SF was significantly influenced by the HAp content during gelation of a mixture of SF and HAp. As a result of MTT assay, the SF/HAp composite hydrogel showed excellent cell proliferation ability. Therefore, it is expected that SF hydrogel containing HAp nanoparticles has a high potential as bone regeneration scaffold.
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, 200/5225 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, Republic of Korea.
authors.forename: Hyung Hwan
authors.initials: HH
authors.lastname: Kim
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, 200/5225 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, Republic of Korea.
authors.forename: Jong Bo
authors.initials: JB
authors.lastname: Park
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, 200/5225 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, Republic of Korea.
authors.forename: Min Ji
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, 200/5225 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, Republic of Korea. Electronic address: nfchempf@snu.ac.kr.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D004298:Dopamine / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D047011:Silk / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid; D017886:Durapatite; D004298:Dopamine
keywords: Hydroxyapatite nanoparticle; Secondary structure; Silk fibroin hydrogel