title: Intranuclear coccidiosis caused by Cyclospora spp. in calves.
abstract: Intranuclear coccidiosis in 3 calves is described. Two calves with no genetic relationship were kept in the same pen, and the third calf was on another farm. The animals suffered from watery diarrhea or severe emaciation. Histologic examination showed epithelial desquamation (cases 1 and 2) or atrophy (case 3) of the jejunal villi. Coccidial meronts or merozoites were present in the nuclei of the majority of villus epithelial cells. There were rare intranuclear macrogametocytes, macrogametes, microgametocytes, microgametes, and oocysts in cases 1 and 2, but these were more easily observed in case 3. Parasite 18S ribosomal RNA sequences from case 1 showed 99.5% sequence identity with Cyclospora sp. Guangzhou 1, which has been found in fecal samples of cattle from China. In addition to the molecular results in one of the cases, the fact that the microgametocytes and oocysts were noticeably smaller in size than those of Eimeria alabamensis in all cases indicates the close association between Cyclospora and the 3 cases described herein.
authors.affiliation: Oita Livestock Hygiene Service Center, Oita, Japan (Yamada)Hokkaido Research Station, National Institute of Animal Health, Toyohira, Sapporo, Japan (Hatama, Ishikawa, Kadota).
authors.forename: Minako
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Oita Livestock Hygiene Service Center, Oita, Japan (Yamada)Hokkaido Research Station, National Institute of Animal Health, Toyohira, Sapporo, Japan (Hatama, Ishikawa, Kadota).
authors.forename: Shinichi
authors.initials: S
authors.lastname: Hatama
authors.affiliation: Oita Livestock Hygiene Service Center, Oita, Japan (Yamada)Hokkaido Research Station, National Institute of Animal Health, Toyohira, Sapporo, Japan (Hatama, Ishikawa, Kadota).
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Ishikawa
authors.affiliation: Oita Livestock Hygiene Service Center, Oita, Japan (Yamada)Hokkaido Research Station, National Institute of Animal Health, Toyohira, Sapporo, Japan (Hatama, Ishikawa, Kadota) kkadota@affrc.go.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Kadota
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology* / Q000473:pathology; D002681:China / Q000453:epidemiology; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D021744:Cyclospora / Q000235:genetics / Q000302:isolation & purification*; D003967:Diarrhea / Q000662:veterinary; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S
chemical_list: D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
keywords: Calves; Cyclospora spp.; Eimeria alabamensis; intranuclear coccidiosis