title: Erythrophagocytosis in Entamoeba histolytica and Entamoeba dispar: a comparative study.
abstract: Entamoeba histolytica is the causative agent of human intestinal and liver amebiasis. The extraordinary phagocytic activity of E. histolytica trophozoites has been accepted as one of the virulence mechanisms responsible for their invasive capacity. The recognition of the noninvasive Entamoeba dispar as a different species has raised the question as to whether the lack of pathogenic potential of this ameba correlates with a limited phagocytic capacity. We have therefore compared the process of erythrophagocytosis in both species by means of light and video microscopy, hemoglobin measurement, and the estimation of reactive oxygen species (ROS). In the present study, we confirmed that E. dispar has lower erythrophagocytic capacity. We also observed by video microscopy a new event of erythrocyte opsonization-like in both species, being more characteristic in E. histolytica. Moreover, E. dispar showed a lower capacity to produce ROS compared with the invasive species and also showed a large population of amoebae that did not engulf any erythrocyte over time. Our results demonstrate that E. histolytica has a higher phagocytic capacity than E. dispar, including a higher rate of production of ROS in the course of ingesting red blood cells.
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, IPN, Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, Delegación Gustavo A. Madero, 07360 Mexico City, DF, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Talamás-Lara
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, IPN, Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, Delegación Gustavo A. Madero, 07360 Mexico City, DF, Mexico.
authors.forename: Bibiana
authors.initials: B
authors.lastname: Chávez-Munguía
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, IPN, Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, Delegación Gustavo A. Madero, 07360 Mexico City, DF, Mexico.
authors.forename: Arturo
authors.initials: A
authors.lastname: González-Robles
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, IPN, Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, Delegación Gustavo A. Madero, 07360 Mexico City, DF, Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Talamás-Rohana
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, IPN, Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, Delegación Gustavo A. Madero, 07360 Mexico City, DF, Mexico.
authors.forename: Lizbeth
authors.initials: L
authors.lastname: Salazar-Villatoro
authors.affiliation: Faculty of Superior Studies Iztacala, Biology, UNAM, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Durán-Díaz
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, IPN, Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, Delegación Gustavo A. Madero, 07360 Mexico City, DF, Mexico.
authors.forename: Adolfo
authors.initials: A
authors.lastname: Martínez-Palomo
mesh_terms: D000818:Animals; D002417:Cattle; D003199:Computer Systems; D004747:Entamoeba / Q000166:cytology*; D004748:Entamoeba histolytica / Q000166:cytology*; D004912:Erythrocytes / Q000469:parasitology*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D018715:Microscopy, Video; D010087:Oxides / Q000378:metabolism; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D006454:Hemoglobins; D010087:Oxides; D017382:Reactive Oxygen Species