title: Cationic surfactant-based colorimetric detection of Plasmodium lactate dehydrogenase, a biomarker for malaria, using the specific DNA aptamer.
abstract: A simple, sensitive, and selective colorimetric biosensor for the detection of the malarial biomarkers Plasmodium vivax lactate dehydrogenase (PvLDH) and Plasmodium falciparum LDH (PfLDH) was demonstrated using the pL1 aptamer as the recognition element and gold nanoparticles (AuNPs) as probes. The proposed method is based on the aggregation of AuNPs using hexadecyltrimethylammonium bromide (CTAB). The AuNPs exhibited a sensitive color change from red to blue, which could be seen directly with the naked eye and was monitored using UV-visible absorption spectroscopy and transmission electron microscopy (TEM). The reaction conditions were optimized to obtain the maximum color intensity. PvLDH and PfLDH were discernible with a detection limit of 1.25 pM and 2.94 pM, respectively. The applicability of the proposed biosensor was also examined in commercially available human serum.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Seonghwan
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Manjunatha
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Weejeong
authors.initials: W
authors.lastname: Jeon
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Changill
authors.initials: C
authors.lastname: Ban
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D057230:Limit of Detection; D016778:Malaria, Falciparum / Q000097:blood* / Q000175:diagnosis / Q000382:microbiology; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010963:Plasmodium falciparum / Q000201:enzymology / Q000302:isolation & purification*; D015800:Protozoan Proteins / Q000097:blood*
chemical_list: D052157:Aptamers, Nucleotide; D015800:Protozoan Proteins; D006046:Gold; D007770:L-Lactate Dehydrogenase