title: Determination of N-acetylation degree in chitosan using Raman spectroscopy.
abstract: Application of Raman spectroscopy in determination of the acetylation degree (DA) of chitosan has been developed. The spectra of several chitosan samples characterized by different DD (degree of deacetylation) in the range 50-100% have been measured. The integral intensities of the bands assigned to the vibrations of amine group and glucosidic ring were used to calculate the DA from the intensity ratio. The assignment of the bands to the respective normal modes of chitosan was based on the DFT quantum chemical calculations. This method has a number of advantages over other techniques. It is fast and does not require purification of the sample nor require dissolution of the chitosan in any solvent.
authors.affiliation: Department of Bioorganic Chemistry, Institute of Chemistry and Food Technology, Faculty of Engineering and Economics, Wrocław University of Economics, Komandorska 118/120, 53-345 Wrocław, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Zając
authors.affiliation: Department of Bioorganic Chemistry, Institute of Chemistry and Food Technology, Faculty of Engineering and Economics, Wrocław University of Economics, Komandorska 118/120, 53-345 Wrocław, Poland; Institute of Low Temperature and Structure Research, Polish Academy of Sciences, P.O. Box 1410, 50-950 Wrocław 2, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Hanuza
authors.affiliation: Department of Bioorganic Chemistry, Institute of Chemistry and Food Technology, Faculty of Engineering and Economics, Wrocław University of Economics, Komandorska 118/120, 53-345 Wrocław, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Wandas
authors.affiliation: Department of Bioorganic Chemistry, Institute of Chemistry and Food Technology, Faculty of Engineering and Economics, Wrocław University of Economics, Komandorska 118/120, 53-345 Wrocław, Poland. Electronic address: lucyna.dyminska@ue.wroc.pl.
authors.forename: L
authors.initials: L
authors.lastname: Dymińska
mesh_terms: D000107:Acetylation; D048271:Chitosan / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D011789:Quantum Theory; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D048271:Chitosan
keywords: Chitosan; Deacetylation degree; IR spectroscopy; Quantum chemical calculations; Raman spectroscopy