title: The application of autologous platelet‑rich plasma gel in cartilage regeneration.
abstract: Cartilage defect caused by disease or trauma remains a challenge for surgeons, owning to the limited healing capacity of cartilage tissues. Cartilage tissue engineering provides a novel approach to address this issue, and appears promising for patients with cartilage defects. The cell scaffold, as one of the three key elements of tissue engineering, plays an important role in cartilage tissue engineering. Platelet‑rich plasma (PRP), which is a fraction of the plasma containing multiple growth factors, has become a major research focus in the context of its use as a bioactive scaffold for tissue engineering. Therefore, we investigated the value of using PRP scaffolds combined with chondrocytes in cartilage tissue engineering. In this study, we examined the levels of growth factors in PRP, and the effects of PRP on cell proliferation and matrix synthesis in rabbit chondrocytes cultured in PRP. Short-term in vitro culture followed by long‑term in vivo implantation was performed to evaluate the chondrogenesis of neocartilage in vivo. The results show that PRP may provide a suitable environment for the proliferation and maturation of chondrocytes, and can be used as a promising bioactive scaffold for cartilage regeneration.
authors.affiliation: Department of Plastic Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Xie
authors.affiliation: Department of Plastic Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
authors.forename: Lanjun
authors.initials: L
authors.lastname: Nie
authors.affiliation: Department of Plastic Surgery, Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
authors.forename: Gan
authors.initials: G
authors.lastname: Shen
authors.affiliation: Department of Plastic Surgery, Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Department of Plastic Surgery, Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Plastic Surgery, Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Ge
authors.affiliation: Department of Plastic Surgery, Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tan
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism; D020219:Chondrogenesis; D008855:Microscopy, Electron, Scanning; D053657:Platelet-Rich Plasma; D011817:Rabbits; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds