title: Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population.
abstract: Retroviral transformation has been associated with pro-proliferative oncogenic signaling in human cells. The current study demonstrates that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells (ALDH1+), which is associated with increased steady-state levels of cancer stem cell populations. Furthermore, this retroviral-induced enhancement of cancer cell growth in vitro was also accompanied by a significant increase in xenograft tumor growth rate in vivo. The retroviral induced increases in cancer cell growth rate were partially inhibited by treatment with 100 U/ml polyethylene glycol-conjugated-(PEG)-superoxide dismutase and/or PEG-catalase. These results show that retroviral infection of MDA-MB231 human breast cancer cells is capable of enhancing cell proliferation and cancer stem cell populations as well as suggesting that modulation of reactive oxygen species-induced pro-survival signaling pathways may be involved in these effects.
authors.affiliation: Department of Health and Human Physiology, University of Iowa, Iowa City, IA 52240, United States ; Free Radical and Radiation Biology Program, Department of Radiation Oncology, Holden Comprehensive Cancer Center, Carver College of Medicine, University of Iowa, Iowa City, IA 52240, United States.
authors.forename: Lauren J
authors.initials: LJ
authors.lastname: Wegman-Points
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, United States ; Free Radical and Radiation Biology Program, Department of Radiation Oncology, Holden Comprehensive Cancer Center, Carver College of Medicine, University of Iowa, Iowa City, IA 52240, United States.
authors.forename: Melissa L T
authors.initials: ML
authors.lastname: Teoh-Fitzgerald
authors.affiliation: Free Radical and Radiation Biology Program, Department of Radiation Oncology, Holden Comprehensive Cancer Center, Carver College of Medicine, University of Iowa, Iowa City, IA 52240, United States ; University of Pittsburg, United States.
authors.forename: Gaowei
authors.initials: G
authors.lastname: Mao
authors.affiliation: Free Radical and Radiation Biology Program, Department of Radiation Oncology, Holden Comprehensive Cancer Center, Carver College of Medicine, University of Iowa, Iowa City, IA 52240, United States ; Northwestern University Medical School, United States.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Free Radical and Radiation Biology Program, Department of Radiation Oncology, Holden Comprehensive Cancer Center, Carver College of Medicine, University of Iowa, Iowa City, IA 52240, United States.
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Fath
authors.affiliation: Free Radical and Radiation Biology Program, Department of Radiation Oncology, Holden Comprehensive Cancer Center, Carver College of Medicine, University of Iowa, Iowa City, IA 52240, United States.
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Spitz
authors.affiliation: Free Radical and Radiation Biology Program, Department of Radiation Oncology, Holden Comprehensive Cancer Center, Carver College of Medicine, University of Iowa, Iowa City, IA 52240, United States.
authors.forename: Frederick E
authors.initials: FE
authors.lastname: Domann
mesh_terms: D000080924:Aldehyde Dehydrogenase 1 Family; D000818:Animals; D001943:Breast Neoplasms / Q000201:enzymology / Q000473:pathology* / Q000821:virology; D002374:Catalase / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000201:enzymology* / Q000821:virology; D011092:Polyethylene Glycols / Q000494:pharmacology; D050697:Retinal Dehydrogenase / Q000378:metabolism*; D012190:Retroviridae / Q000502:physiology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D014183:Transplantation, Heterologous
chemical_list: D007527:Isoenzymes; C070295:catalase-polyethylene glycol; D011092:Polyethylene Glycols; D002374:Catalase; D013482:Superoxide Dismutase; C077064:polyoxyethylene-superoxide dismutase; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; C515028:ALDH1A1 protein, mouse; D050697:Retinal Dehydrogenase
keywords: Aldehyde dehydrogenase positive; Antioxidant enzymes; Mammary cancer; Oxidative stress; Stem cells; Viral carcinogenesis