title: Roles played by acidic lipids in HIV-1 Gag membrane binding.
abstract: The MA domain mediates plasma membrane (PM) targeting of HIV-1 Gag, leading to particle assembly at the PM. The interaction between MA and acidic phospholipids, in addition to N-terminal myristoyl moiety, promotes Gag binding to lipid membranes. Among acidic phospholipids, PI(4,5)P2, a PM-specific phosphoinositide, is essential for proper HIV-1 Gag localization to the PM and efficient virus particle production. Recent studies further revealed that MA-bound RNA negatively regulates HIV-1 Gag membrane binding and that PI(4,5)P2 is necessary to overcome this RNA-imposed block. In this review, we will summarize the current understanding of Gag-membrane interactions and discuss potential roles played by acidic phospholipids.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, United States.
authors.forename: Balaji
authors.initials: B
authors.lastname: Olety
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, United States. Electronic address: akiraono@umich.edu.
authors.forename: Akira
authors.initials: A
authors.lastname: Ono
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D015488:HIV Antigens / Q000737:chemistry / Q000378:metabolism; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D021962:Membrane Microdomains / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000378:metabolism*
chemical_list: D015488:HIV Antigens; D008055:Lipids; D010716:Phosphatidylinositols; D010743:Phospholipids; D012367:RNA, Viral; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1
keywords: Acyl chains; HIV-1 Gag; Membrane binding; PI(4,5)P(2); Virus assembly