title: Antitumor effects of baculovirus-infected dendritic cells against human pancreatic carcinoma.
abstract: Recently, we showed that baculovirus (BV)-infected dendritic cells (DCs) (BV-DCs) induced antitumor immunity against established tumors in mice. These antitumor effects were CD8(+) T-cell and natural killer (NK) cell dependent but CD4(+) T-cell independent. In the current study, we examined the antitumor effect of BV-DCs on human pancreatic cancer cells (AsPC-1). After treatment with BV-infected bone marrow-derived dendritic cells (BMDCs), human pancreatic tumors caused by AsPC-1 cells in a nude mouse model were significantly reduced in size, and the survival of the mice was improved compared with that of non-immature BMDC (iDC)- and BV-DC-immunized mice. We also found that wild-type BV could activate human DCs (HDCs) and that NK cells were activated by BV-infected HDCs (BHDCs). Our findings show that BV-DCs can induce antitumor immunity, which paves the way for the use of this technique as an effective tool for DC immunotherapy against malignancies.
authors.affiliation: Department of Life and Environmental Sciences, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Fujihira
authors.affiliation: Department of Life and Environmental Sciences, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: High Technology Research Center, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba, Japan.
authors.forename: M O
authors.initials: MO
authors.lastname: Chang
authors.affiliation: Department of Life and Environmental Sciences, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Moriyama
authors.affiliation: Immunology Program, Benaroya Research Institute at Virginia Mason, Seattle, WA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kitajima
authors.affiliation: 1] Department of Life and Environmental Sciences, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba, Japan [2] High Technology Research Center, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba, Japan [3] Research Institute, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takaku
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology*; D005260:Female; D006801:Humans; D007167:Immunotherapy; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D016019:Survival Analysis