title: miR-145 downregulates the expression of cyclin-dependent kinase 6 in human cervical carcinoma cells.
abstract: The present study aimed to investigate the effect of the inhibition of miR-145 on cyclin-dependent protein kinase 6 (CDK6) and the proliferation of human cervical carcinoma cells. The miR-145 sequence was synthesized and cloned into pcDNA™6.2-GW to construct the recombinant plasmid pcDNA6.2-GW-miR-145. HeLa cells were divided into the micro (mi)R-145, normal control and blank groups. The transcription levels of miR-145 and CDK6 were detected using quantitative polymerase chin reaction and western blot analysis was used to examine the CDK6 protein expression. In addition, the inhibitory effect of miR-145 on the proliferation of HeLa cells was measured by an MTT assay. The recombinant plasmid pcDNA6.2-GW-miR-145 was successfully constructed and used to transfect the HeLa cells in the MiR-145 group. The miR-145 expression level in the miR-145 group was significantly higher than that in the blank group. The CDK6 expression level in miR-145 group was significantly lower than that in the blank group. Furthermore, miR-145 inhibited the proliferation of HeLa cells. In conclusion, miR-145 overexpression suppresses the expression of CDK6 and inhibits the proliferative ability of HeLa cells.
authors.affiliation: Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, Shaanxi 716000, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, Shaanxi 716000, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, Shaanxi 716000, P.R. China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, Shaanxi 716000, P.R. China.
authors.forename: Manpeng
authors.initials: M
authors.lastname: Huo
authors.affiliation: Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, Shaanxi 716000, P.R. China.
authors.forename: Mingtao
authors.initials: M
authors.lastname: Mu
authors.affiliation: Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, Shaanxi 716000, P.R. China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, Shaanxi 716000, P.R. China.
authors.forename: Jiming
authors.initials: J
authors.lastname: Han
keywords: cervical carcinoma; cyclin-dependent kinase 6; eukaryotic expression vector; miRNA-145