title: Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass.
abstract: Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L. The inhibition kinetics of cellulase by vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase.
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Benkun
authors.initials: B
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: yhwan@home.ipe.ac.cn.
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Wan
mesh_terms: D001547:Benzaldehydes / Q000494:pharmacology*; D018533:Biomass; D002480:Cellulase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D014242:Trichoderma / Q000201:enzymology
chemical_list: D001547:Benzaldehydes; D002482:Cellulose; C100058:vanillin; D002480:Cellulase
keywords: Cellulase; Inhibition kinetics; Inhibitory group; Vanillin