title: The complete nucleotide sequence of a novel partitivirus isolated from the plant pathogenic fungus Verticillium albo-atrum.
abstract: We have characterized the bisegmented genome of a novel double-stranded RNA (dsRNA) virus isolated from the plant pathogenic fungus Verticillium albo-atrum. We determined that its larger segment (dsRNA1) was 1747 base pairs in length and potentially encoded an RNA-dependent RNA polymerase of 539 amino acids, whereas the smaller segment (dsRNA2) was 1517 base pairs long and was predicted to encode a capsid protein of 435 amino acids. Homology searches and phylogenetic analysis confirmed that, as expected from its dsRNA banding profile, the identified virus was a new member of the family Partitiviridae, and we have therefore designated it V. a lbo- a trum partitivirus 1 (VaaPV1). This is the first report of a mycovirus identified in V. albo-atrum.
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga, Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental "La Mayora", 29750, Algarrobo-Costa, Málaga, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Cañizares
authors.forename: Encarnación
authors.initials: E
authors.lastname: Pérez-Artés
authors.forename: María D
authors.initials: MD
authors.lastname: García-Pedrajas
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D012328:RNA Viruses / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016415:Sequence Alignment; D020172:Verticillium / Q000821:virology*
chemical_list: D036022:Capsid Proteins