title: Clock Rooting Further Demonstrates that Guinea 2014 EBOV is a Member of the Zaïre Lineage.
abstract: While initial phylogenetic analyses concluded to Guinea 2014 EBOV falling outside the Zaïre lineage (ZEBOV), a recent re-analysis of the same dataset by Dudas and Rambaut (2014) suggested that Guinea 2014 EBOV actually is ZEBOV. Under the same hypothesis as used by these authors (the molecular clock hypothesis), we reinforce their conclusion by providing a statistical assessment of the location of the root of the Zaïre lineage. Our analysis unambiguously supports Guinea 2014 EBOV as a member of the Zaïre lineage. In addition, we also show that some uncertainty exists so as to the location of the root of the genus Ebolavirus. We release the software we used for these re-analyses. RootAnnotator allows for the easy determination of branch root posterior probability from any posterior sample of clocked trees and is freely available at http://sourceforge.net/projects/rootannotator/.
authors.affiliation: Robert Koch-Institute, Berlin, Germany.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Calvignac-Spencer
authors.affiliation: Robert Koch-Institute, Berlin, Germany.
authors.forename: Jakob M
authors.initials: JM
authors.lastname: Schulze
authors.affiliation: Robert Koch-Institute, Berlin, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Zickmann
authors.affiliation: Robert Koch-Institute, Berlin, Germany.
authors.forename: Bernhard Y
authors.initials: BY
authors.lastname: Renard