title: Closely related NAC transcription factors of tomato differentially regulate stomatal closure and reopening during pathogen attack.
abstract: To restrict pathogen entry, plants close stomata as an integral part of innate immunity. To counteract this defense, Pseudomonas syringae pv tomato produces coronatine (COR), which mimics jasmonic acid (JA), to reopen stomata for bacterial entry. It is believed that abscisic acid (ABA) plays a central role in regulating bacteria-triggered stomatal closure and that stomatal reopening requires the JA/COR pathway, but the downstream signaling events remain unclear. We studied the stomatal immunity of tomato (Solanum lycopersicum) and report here the distinct roles of two homologous NAC (for NAM, ATAF1,2, and CUC2) transcription factors, JA2 (for jasmonic acid2) and JA2L (for JA2-like), in regulating pathogen-triggered stomatal movement. ABA activates JA2 expression, and genetic manipulation of JA2 revealed its positive role in ABA-mediated stomatal closure. We show that JA2 exerts this effect by regulating the expression of an ABA biosynthetic gene. By contrast, JA and COR activate JA2L expression, and genetic manipulation of JA2L revealed its positive role in JA/COR-mediated stomatal reopening. We show that JA2L executes this effect by regulating the expression of genes involved in the metabolism of salicylic acid. Thus, these closely related NAC proteins differentially regulate pathogen-induced stomatal closure and reopening through distinct mechanisms.
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Minmin
authors.initials: M
authors.lastname: Du
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Qingzhe
authors.initials: Q
authors.lastname: Zhai
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Hongshuang
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Liuhua
authors.initials: L
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Institute of Vegetable, Qingdao Academy of Agricultural Sciences, Qingdao 266100, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Huang
authors.affiliation: Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
authors.forename: Chang-Bao
authors.initials: CB
authors.lastname: Li
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Jianing
authors.initials: J
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Le
authors.initials: L
authors.lastname: Kang
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jingfu
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China cyli@genetics.ac.cn.
authors.forename: Chuanyou
authors.initials: C
authors.lastname: Li
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D054884:Host-Pathogen Interactions; D007192:Indenes / Q000378:metabolism; D018551:Solanum lycopersicum / Q000166:cytology / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010937:Plant Growth Regulators / Q000378:metabolism*; D057865:Plant Immunity; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D054046:Plant Stomata / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D044224:Pseudomonas syringae / Q000502:physiology; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D003517:Cyclopentanes; D007192:Indenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; C058487:coronatine; C011006:jasmonic acid; D000040:Abscisic Acid; D020156:Salicylic Acid