title: [Vimentin gene transfection promotes the invasion of bladder cancer SW780 cells].
abstract: OBJECTIVE
  To construct a recombinant pcDNA3.1-vimentin and transfect it into human bladder carcinoma SW780 cells, and investigate the effect of vimentin on the invasion of SW780 cells.
  
  
  METHODS
  Human vimentin cDNA was amplified by PCR and inserted into vector pcDNA3.1(-). After restriction analysis and sequencing, the recombinant plasmid pcDNA3.1-vimentin was transfected into SW780 cells. The expressions of vimentin and matrix metalloproteinase 9 (MMP-9) were detected using Western blotting, and the secretion of MMP-9 in SW780 cells was tested using gelatin zymography. The invasion of SW780 cells was examined by Transwell™ assay.
  
  
  RESULTS
  Enzyme digestion and sequencing analysis confirmed the successful cloning of pcDNA3.1-vimentin. Western blotting demonstrated that both vimentin and MMP-9 were up-regulated in SW780 cells containing pcDNA3.1-vimentin. Gelatin zymography indicated that vimentin increased MMP-9 secretion of SW780 cells. Transwell™ assay showed vimentin obviously promoted the invasion of SW780 cells.
  
  
  CONCLUSIONS
  Plasmid pcDNA3.1-vimentin was constructed successfully and expressed in SW780 cells. Up-regulation of vimentin could promote the invasion of SW780 cells. Up-regulation and over-secretion of MMP-9 may be involved in vimentin-enhanced invasion of SW780 cells.
authors.affiliation: Department of Urology, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Lu
authors.affiliation: Department of Physiology, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Ding
authors.affiliation: Department of Radiology, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D014162:Transfection; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D014746:Vimentin / Q000235:genetics / Q000378:metabolism*
chemical_list: D014746:Vimentin; D020780:Matrix Metalloproteinase 9