title: A bioorthogonal (68)Ga-labelling strategy for rapid in vivo imaging.
abstract: Herein, we describe a fast and robust method for achieving (68)Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels-Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the method within a two-step pretargeting strategy.
authors.affiliation: Comprehensive Cancer Imaging Centre, Department of Surgery & Cancer, Imperial College London, Hammersmith Hospital, London W12 0NN, UK. e.aboagye@imperial.ac.uk.
authors.forename: Helen L
authors.initials: HL
authors.lastname: Evans
authors.forename: Quang-Dé
authors.initials: QD
authors.lastname: Nguyen
authors.forename: Laurence S
authors.initials: LS
authors.lastname: Carroll
authors.forename: Maciej
authors.initials: M
authors.lastname: Kaliszczak
authors.forename: Frazer J
authors.initials: FJ
authors.lastname: Twyman
authors.forename: Alan C
authors.initials: AC
authors.lastname: Spivey
authors.forename: Eric O
authors.initials: EO
authors.lastname: Aboagye
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry* / Q000276:immunology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D000068818:Cetuximab; D066246:ErbB Receptors / Q000276:immunology; D005710:Gallium Radioisotopes; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D051379:Mice; D049268:Positron-Emission Tomography; D013997:Time Factors
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D005710:Gallium Radioisotopes; D066246:ErbB Receptors; D000068818:Cetuximab