title: [The expression of SIRT1 in brainstem of rats in different types of hypoxia and its significance].
abstract: OBJECTIVE
  To explore the correlation between SIRT1 expression in brainstem and apnea hypopnea index and the effect of hypoxia on SIRT1 expression in rats.
  
  
  METHODS
  Thirty-three healthy Sprague-Dawley(SD) rats underwent sleep monitoring and SIRT1 protein in brainstems was measured by Western blot. The correlation of sleep apnea index and SIRT1 protein level in brainstem was analysed. Another 36 adult male SD rats were randomly and equally divided into 4 groups: normal control (NC) group, continuous hypoxia (CH) group, intermittent hypoxia (IH) group and intermittent hypoxia with hypercapnia (IHH) group. The expression of SIRT1 protein of the lower brainstems of the 4 groups were measured by Western blot.
  
  
  RESULTS
  The level of SIRT1 in the brainstem was negatively correlated with the frequency of spontaneous sleep apnea in REM(r = -0.617, P < 0.001) while positively correlated with the frequency of post sigh sleep apnea in REM (r = 0.535, P < 0.01). The relative expression level of SIRT1 in NC, CH, IH, IHH group were (1.4 ± 1.0), (1.4 ± 0.6), (2.5 ± 1.0) , (1.6 ± 1.2), respectively. There was a significant difference in the SIRT1 level among groups (F = 2.964, P < 0.05) .
  
  
  CONCLUSIONS
  The rats with lower level of SIRT1 may have lower anti-oxidative stress capability which results in higher frequency of spontaneous sleep apnea. Under the circumstance of hypoxia, the fact that the expression of SIRT1 protein was elevated may be a protective mechanism.
authors.affiliation: Department of respiratory and critical care medicine, Peking University First Hospital, Beijing 100034, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Department of respiratory and critical care medicine, Peking University First Hospital, Beijing 100034, China.
authors.forename: Qimin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of respiratory and critical care medicine, Peking University First Hospital, Beijing 100034, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of respiratory and critical care medicine, Peking University First Hospital, Beijing 100034, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of respiratory and critical care medicine, Peking University First Hospital, Beijing 100034, China. Email: wangguangfa@hotmail.com.
authors.forename: Guangfa
authors.initials: G
authors.lastname: Wang
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D000860:Hypoxia; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D056564:Sirtuin 1 / Q000378:metabolism*; D012891:Sleep Apnea Syndromes / Q000378:metabolism*
chemical_list: C488368:Sirt1 protein, rat; D056564:Sirtuin 1