title: Early posttransplant changes in circulating endothelial microparticles in patients with kidney transplantation.
abstract: BACKGROUND
  Endothelial microparticles (EMPs) are membrane vesicles shed from endothelial cell in response to injury, activation or apoptosis. Kidney transplantation (KTx) is the treatment of choice for patients with end stage kidney disease (ESKD). The aim of this study was to analyze changes in EMP and serum creatinine (SCr) in patients following KTx.
  
  
  METHODS
  Blood was periodically collected from patients before (pre-KTx) and after KTx for two months. EMPs were identified as CD31(+)/CD42b(-) microparticles and quantified by fluorescence-activated cell scanning.
  
  
  RESULTS
  This study included 213 KTx, 14 kidney/pancreas (KPTx) recipients and 60 healthy donors prior to donation. The recipients were divided into 5 groups based on the cause of ESKD. No differences in the quantity of circulating EMP were seen in the pre-KPTx or KTx recipient sera and healthy donor sera. Patients with ESKD secondary to diabetes mellitus, obstructive/inherited kidney disease and autoimmune disease had a decrease in both circulating EMP and SCr by day 60 after KTx.
  
  
  CONCLUSIONS
  Reduction in both circulating EMP and SCr was seen after kidney KTx in patients with selective ESKD.
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
authors.forename: Zahida
authors.initials: Z
authors.lastname: Qamri
authors.affiliation: Comprehensive Transplant Center, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
authors.forename: Ronald
authors.initials: R
authors.lastname: Pelletier
authors.affiliation: Comprehensive Transplant Center, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
authors.forename: Jamison
authors.initials: J
authors.lastname: Foster
authors.affiliation: Comprehensive Transplant Center, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Comprehensive Transplant Center, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
authors.forename: Hammam
authors.initials: H
authors.lastname: Momani
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
authors.forename: Kyle
authors.initials: K
authors.lastname: Ware
authors.affiliation: Comprehensive Transplant Center, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
authors.forename: Jon
authors.initials: J
authors.lastname: Von Visger
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
authors.forename: Anjali
authors.initials: A
authors.lastname: Satoskar
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH, United States.
authors.forename: Tibor
authors.initials: T
authors.lastname: Nadasdy
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH, United States. Electronic address: sergey.brodsky@osumc.edu.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Brodsky
mesh_terms: D000328:Adult; D064591:Allografts; D001327:Autoimmune Diseases / Q000097:blood; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D003404:Creatinine / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood; D042783:Endothelial Cells / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006084:Graft Rejection / Q000473:pathology; D006801:Humans; D007668:Kidney / Q000601:surgery*; D007674:Kidney Diseases / Q000097:blood / Q000601:surgery; D007676:Kidney Failure, Chronic / Q000097:blood / Q000601:surgery; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019038:Platelet Glycoprotein GPIb-IX Complex; D003404:Creatinine
keywords: Allograft rejection; Circulating microparticle; Kidney transplantation