title: Light microscope alignment methods.
abstract: Instructions for proper alignment for each of the most common wide field light microscopy contrast techniques are presented: bright field, polarization, dark field, phase contrast, differential interference contrast, and fluorescence. These are then followed by methods on photomicrography, measurement, and cleaning. An extensive set of Notes provides practical tips for each of these methods.
authors.affiliation: Microscopy Services Laboratory, Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, 101 Manning Drive, 27599, Chapel Hill, NC, USA, rml@med.unc.edu.
authors.forename: Robert
authors.initials: R
authors.lastname: Bagnell
mesh_terms: D008027:Light; D008853:Microscopy / Q000295:instrumentation*; D008856:Microscopy, Fluorescence; D055620:Optical Phenomena