title: Detection of cancer cells using triplex DNA molecular beacons based on expression of enhanced green fluorescent protein (eGFP).
abstract: A novel strategy was proposed for Ramos cell detection by combining the expression of enhanced green fluorescent protein (eGFP) with the cell aptamer recognition and the triplex molecular beacons. This system was successfully applied to cancer cell detection with high sensitivity and specificity.
authors.affiliation: Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China. shushzhang@126.com.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Xi
authors.forename: Xindong
authors.initials: X
authors.lastname: Wang
authors.forename: Shiyun
authors.initials: S
authors.lastname: Ai
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D015345:Oligonucleotide Probes; C485184:enhanced green fluorescent protein; C051802:triplex DNA; D049452:Green Fluorescent Proteins; D004247:DNA