title: Electroporation markedly improves Sleeping Beauty transposon-induced tumorigenesis in mice.
abstract: The Sleeping Beauty (SB) transposon system is an important tool for genetic studies. It is used to insert a gene of interest into the host chromosome, thus enabling permanent gene expression. However, this system is less useful in higher eukaryotes because the transposition frequency is low. Efforts to improve the efficacy of the SB transposon system have focused on the method of gene delivery, but although electroporation has recently attracted much attention as an in vivo gene delivery tool, the simultaneous use of electroporation and the SB transposon system has not been studied for gene transfer in mice. In this study, electroporation was used in a model of SB transposon-induced insertional tumorigenesis. Electroporation increased the rate of tumor development to three times that of the control group. There was no difference in phenotype between tumors induced with the SB transposon system alone and those induced by the SB transposon and electroporation. Electroporation therefore may be an efficient means of improving the efficacy of gene transfer via the SB transposon system.
authors.affiliation: 1] Comparative Pathology Core, Asan Institute for Life Sciences, Seoul, Korea [2] Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Jung
authors.affiliation: 1] Comparative Pathology Core, Asan Institute for Life Sciences, Seoul, Korea [2] Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: 1] Comparative Pathology Core, Asan Institute for Life Sciences, Seoul, Korea [2] Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: H-K
authors.initials: HK
authors.lastname: Park
authors.affiliation: 1] Comparative Pathology Core, Asan Institute for Life Sciences, Seoul, Korea [2] Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: W
authors.initials: W
authors.lastname: Jo
authors.affiliation: 1] Comparative Pathology Core, Asan Institute for Life Sciences, Seoul, Korea [2] Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Jang
authors.affiliation: 1] Comparative Pathology Core, Asan Institute for Life Sciences, Seoul, Korea [2] Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: J-E
authors.initials: JE
authors.lastname: Ryu
authors.affiliation: Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: W-J
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: 1] Comparative Pathology Core, Asan Institute for Life Sciences, Seoul, Korea [2] Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: E-S
authors.initials: ES
authors.lastname: Yu
authors.affiliation: 1] Comparative Pathology Core, Asan Institute for Life Sciences, Seoul, Korea [2] Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: W-C
authors.initials: WC
authors.lastname: Son
mesh_terms: D000818:Animals; D001706:Biopsy; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004251:DNA Transposable Elements; D004195:Disease Models, Animal; D018274:Electroporation; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D007150:Immunohistochemistry; D051379:Mice; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D049268:Positron-Emission Tomography; D047368:Tumor Burden
chemical_list: D004251:DNA Transposable Elements