title: SPARC/osteonectin is involved in metastatic process to the lung during melanoma progression.
abstract: The existence of a "metastasis gene signature" that predisposes primary breast cancer cells to metastasize to the lungs has been recently highlighted by gene expression profiling studies. The combination of genes responsible for this process includes genes encoding several metalloproteinases as well as the gene encoding SPARC (secreted protein acidic and rich in cysteine)/osteonectin. SPARC is involved in normal tissue remodeling as it regulates the deposition of extracellular matrix, but also plays a role in neoplastic transformation. Aberrant SPARC expression has been detected both in stromal cells associated with cancer and in cancer cells. The main aim of this study was to investigate whether or not SPARC might be involved in directing metastasis of other types of cancer to the lung. We constructed a tissue microarray containing lung metastases from a variety of primary tumors in different organs and used immunohistochemistry to assess SPARC expression. We found SPARC overexpressed mainly in lung metastases from melanoma. We then assessed the expression of SPARC mRNA and protein in metastatic melanoma from different anatomic sites and in their corresponding primary tumors, and found that it is overexpressed in lung metastases. Our data strongly support the hypothesis that SPARC is involved in directing melanoma metastases specifically to the lung, which underpins its potential as prognostic marker and novel target for specific therapy.
authors.affiliation: Pathology Unit, Istituto Nazionale Tumori Fondazione, "G. Pascale", via Mariano Semmola, 80131, Naples, Italy.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Botti
authors.forename: Giosuè
authors.initials: G
authors.lastname: Scognamiglio
authors.forename: Laura
authors.initials: L
authors.lastname: Marra
authors.forename: Francesca
authors.initials: F
authors.lastname: Collina
authors.forename: Maurizio
authors.initials: M
authors.lastname: Di Bonito
authors.forename: Margherita
authors.initials: M
authors.lastname: Cerrone
authors.forename: Bruna
authors.initials: B
authors.lastname: Grilli
authors.forename: Annamaria
authors.initials: A
authors.lastname: Anniciello
authors.forename: Renato
authors.initials: R
authors.lastname: Franco
authors.forename: Franco
authors.initials: F
authors.lastname: Fulciniti
authors.forename: Paolo Antonio
authors.initials: PA
authors.lastname: Ascierto
authors.forename: Monica
authors.initials: M
authors.lastname: Cantile
mesh_terms: D000328:Adult; D000368:Aged; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D008545:Melanoma / Q000556:secondary*; D008875:Middle Aged; D015676:Osteonectin / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D015676:Osteonectin