title: Effect of chlorhexidine, povidone iodine, and ozone on microorganisms in dental aerosols: randomized double-blind clinical trial.
abstract: OBJECTIVE
  Dental handpieces, ultrasonic scalers, air polishers, air abrasion units produce the most visible aerosols. The objective of this study was to assess the effect of chlorhexidine (CHX), povidone iodine (PI), and ozone (OZ) on the microorganisms in dental aerosols.
  
  
  METHODS
  A total of 60 patients were included in this study, they were randomly assigned into three groups and were subjected to scaling before and after rinsing with 0.2% CHX, 1% PI or irrigation with OZ. Blood agar plates were used to collect the gravitometric settling of aerosols and were sent for aerobic and anaerobic culture.
  
  
  RESULTS
  The results demonstrated high percentage reduction of aerobic and anaerobic colony forming units (CFUs) in all three groups. In aerobic CFUs, CHX showed the highest reduction (57%) at mask position whereas at chest position and at 9 ft, PI showed higher CFU reductions (37% and 47%, respectively). In anaerobic CFUs, CHX showed the highest percentage of reduction at chest level (43%) and at 9 ft (44%).
  
  
  CONCLUSIONS
  CHX, PI and OZ showed similar effects in reducing aerobic and anaerobic CFU's at the chest mask and at 9 ft. OZ can be used as a preprocedural agent, considering its beneficial effects.
authors.forename: Ravleen
authors.initials: R
authors.lastname: Kaur
authors.forename: Inderjot
authors.initials: I
authors.lastname: Singh
authors.affiliation: Department of Periodontics, College of Dental Sciences, Davangere, Karnataka, India.
authors.forename: K L
authors.initials: KL
authors.lastname: Vandana
authors.forename: Rajendra
authors.initials: R
authors.lastname: Desai
mesh_terms: D000328:Adult; D000336:Aerosols; D000391:Air Microbiology; D001419:Bacteria / Q000187:drug effects*; D002710:Chlorhexidine / Q000494:pharmacology*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology*; D010126:Ozone / Q000494:pharmacology*; D011206:Povidone-Iodine / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000336:Aerosols; D010126:Ozone; D011206:Povidone-Iodine; D002710:Chlorhexidine