title: Moesin and stress-induced phosphoprotein-1 are possible sero-diagnostic markers of psoriasis.
abstract: To identify diagnostic markers for psoriasis vulgaris and psoriatic arthritis, autoantibodies in sera from psoriasis vulgaris and psoriatic arthritis patients were screened by two-dimensional immunoblotting (2D-IB). Based on 2D-IB and MADLI TOF/TOF-MS analyses, eleven proteins each in psoriasis vulgaris and psoriatic arthritis were identified as autoantigens. Furthermore, serum levels of moesin, keratin 17 (K17), annexin A1 (ANXA1), and stress-induced phophoprotein-1 (STIP1), which were detected as autoantigens, were studied by dot blot analysis with psoriasis patients and healthy controls. The levels of moesin and STIP1 were significantly higher in sera from patients with psoriasis vulgaris than in the controls (moesin: P<0.05, STIP1: P<0.005). The area under the curve (AUC) for moesin and STIP1 between patients with psoraisis vulgaris and controls was 0.747 and 0.792, respectively. STIP1 and K17 levels were significantly higher in sera from patients with psoriatic arthritis than in those with psoriasis vulgaris (P<0.05 each). The AUC for STIP1 and K17 between patients with psoriatic arthritis and psoriasis vulgaris was 0.69 and 0.72, respectively. The STIP1 or moesin, CK17 serum level was not correlated with disease activity of psoriasis patients. These data suggest that STIP1 and moesin may be novel and differential sero-diagnostic markers for psoriasis vulgaris and psoriatic arthritis.
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Maejima
authors.affiliation: Department of Applied Tumor Pathology, Kitasato University Graduate School of Medical Sciences, Sagamihara, Japan; Department of Molecular Diagnostics, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Nagashio
authors.affiliation: Department of Applied Tumor Pathology, Kitasato University Graduate School of Medical Sciences, Sagamihara, Japan; Department of Molecular Diagnostics, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Yanagita
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hamada
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Amoh
authors.affiliation: Department of Applied Tumor Pathology, Kitasato University Graduate School of Medical Sciences, Sagamihara, Japan; Department of Molecular Diagnostics, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Japan.
authors.forename: Kensei
authors.initials: K
authors.lastname: Katsuoka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017305:Annexin A1 / Q000097:blood; D015535:Arthritis, Psoriatic / Q000097:blood / Q000276:immunology; D001324:Autoantigens / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002460:Cell Line; D005260:Female; D006360:Heat-Shock Proteins / Q000097:blood*; D006801:Humans; D053537:Keratin-17 / Q000097:blood; D008297:Male; D008840:Microfilament Proteins / Q000097:blood*; D008875:Middle Aged; D011565:Psoriasis / Q000097:blood* / Q000276:immunology; D012698:Serologic Tests; D055815:Young Adult
chemical_list: D017305:Annexin A1; D001324:Autoantigens; D015415:Biomarkers; D006360:Heat-Shock Proteins; D053537:Keratin-17; D008840:Microfilament Proteins; C079373:STIP1 protein, human; C071058:moesin