title: Innovative pretreatment of sugarcane bagasse using supercritical CO2 followed by alkaline hydrogen peroxide.
abstract: An innovative method for pretreatment of sugarcane bagasse using sequential combination of supercritical CO2 (scCO2) and alkaline hydrogen peroxide (H2O2) at mild conditions is proposed. This method was found to be superior to the individual pretreatment with scCO2, ultrasound, or H2O2 and the sequential combination of scCO2 and ultrasound regarding the yield of cellulose and hemicellulose, almost twice the yield was observed. Pretreatment with scCO2 could obtain higher amount of cellulose and hemicellulose but also acid-insoluble lignin. Pretreatment with ultrasound or H2O2 could partly depolymerize lignin, however, could not separate cellulose from lignin. The analysis of liquid products via enzymatic hydrolysis by HPLC and the characterization of the solid residues by SEM revealed strong synergetic effects in the sequential combination of scCO2 and H2O2.
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC.
authors.forename: Duy The
authors.initials: DT
authors.lastname: Phan
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC. Electronic address: cstan@mx.nthu.edu.tw.
authors.forename: Chung-Sung
authors.initials: CS
authors.lastname: Tan
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000494:pharmacology*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D005947:Glucose / Q000032:analysis; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006868:Hydrolysis / Q000187:drug effects; D031786:Saccharum / Q000187:drug effects*; D013696:Temperature
chemical_list: D000468:Alkalies; D002245:Carbon Dioxide; D002482:Cellulose; C027433:bagasse; D006861:Hydrogen Peroxide; D002480:Cellulase; D005947:Glucose
keywords: Alkaline hydrogen peroxide; Consequence pretreatment; Sugarcane bagasse; Ultrasound; scCO(2)