title: Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense.
abstract: A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China; Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, Guangdong, China.
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Han
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
authors.forename: Gar-Lee
authors.initials: GL
authors.lastname: Yue
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China.
authors.forename: Rui-Qi
authors.initials: RQ
authors.lastname: Yue
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
authors.forename: Chung-Lap
authors.initials: CL
authors.lastname: Chan
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
authors.forename: Chun-Hay
authors.initials: CH
authors.lastname: Ko
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
authors.forename: Wing-Shing
authors.initials: WS
authors.lastname: Cheung
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
authors.forename: Ke-Wang
authors.initials: KW
authors.lastname: Luo
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
authors.forename: Chun-Kwok
authors.initials: CK
authors.lastname: Wong
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
authors.forename: Ping-Chung
authors.initials: PC
authors.lastname: Leung
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China; School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China.
authors.forename: Quan-Bin
authors.initials: QB
authors.lastname: Han
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003713:Dendritic Cells / Q000187:drug effects; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D032902:Ganoderma / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D051379:Mice
chemical_list: D005936:Glucans