title: Three-dimensional polycaprolactone scaffold via needleless electrospinning promotes cell proliferation and infiltration.
abstract: Electrospinning has been widely used in fabrication of tissue engineering scaffolds. Currently, most of the electrospun nanofibers performed like a conventional two-dimensional (2D) membrane, which hindered their further applications. Moreover, the low production rate of the traditional needle-electrospinning (NE) also limited the commercialization. In this article, disc-electrospinning (DE) was utilized to fabricate a three-dimensional (3D) scaffold consisting of porous macro/nanoscale fibers. The morphology of the porous structure was investigated by scanning electron microscopy images, which showed irregular pores of nanoscale spreading on the surface of DE polycaprolactone (PCL) fibers. Protein adsorption assessment illustrated the porous structure could significantly enhance proteins pickup, which was 55% higher than that of solid fiber scaffolds. Fibroblasts were cultured on the scaffold. The results demonstrated that DE fiber scaffold could enhance initial cell attachment. In the 7 days of culture, fibroblasts grew faster on DE fiber scaffold in comparison with solid fiber, solvent cast (SC) film and TCP. Fibroblasts on DE fibers showed a stretched shape and integrated with the porous surface tightly. Cells were also found to migrate into the DE scaffold up to 800μm. Results supported the use of DE PCL fibers as a 3D tissue engineering scaffold in soft tissue regeneration.
authors.affiliation: Engineering Research Center of Technical Textiles, Donghua University, Shanghai 201620, China; College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Wu
authors.affiliation: Engineering Research Center of Technical Textiles, Donghua University, Shanghai 201620, China.
authors.forename: Nanfei
authors.initials: N
authors.lastname: He
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Liping
authors.initials: L
authors.lastname: He
authors.affiliation: Engineering Research Center of Technical Textiles, Donghua University, Shanghai 201620, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt.
authors.forename: Hany A
authors.initials: HA
authors.lastname: Ei-Hamshary
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Salem S
authors.initials: SS
authors.lastname: Al-Deyab
authors.affiliation: Engineering Research Center of Technical Textiles, Donghua University, Shanghai 201620, China; Shanghai Normal University, Shanghai 200234, China. Electronic address: kqf@dhu.edu.cn.
authors.forename: Qinfei
authors.initials: Q
authors.lastname: Ke
authors.affiliation: Engineering Research Center of Technical Textiles, Donghua University, Shanghai 201620, China; College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China. Electronic address: xmm@dhu.edu.cn.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D057139:Nanofibers / Q000648:ultrastructure; D011091:Polyesters / Q000494:pharmacology*; D016062:Porosity; D051381:Rats; D012710:Serum Albumin, Bovine / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D012710:Serum Albumin, Bovine
keywords: Disc-electrospinning; Needleless electrospinning; PCL; Porous surface; Three-dimensional scaffold