title: Traditional goat husbandry may substantially contribute to human toxoplasmosis exposure.
abstract: Raising goats in settings that are highly contaminated with oocysts of Toxoplasma gondii may contribute significantly to human exposure to this zoonotic parasite. Increasing consumption of young goats in countries where goats are frequently reared in backyards that are also homes to cats (the definitive host of this parasite) elevates such concern. To date, there has been little attention to either the prevalence or genotypic characteristics of T. gondii isolates in young ruminant food animals in Europe. Here, we estimated the prevalence of T. gondii goat-kids raised in backyards and slaughtered for human consumption during Easter. We collected 181 paired samples of serum and diaphragm. Serum samples were analyzed by enzyme-linked immunosorbent assay for antibodies against T. gondii , and muscle tissues were analyzed by polymerase chain reaction to detect T. gondii DNA. Thirty-two diaphragm samples were also bioassayed in mice, and the isolates were genotyped using microsatellite markers. The overall seroprevalence of T. gondii infection in goat-kids was 33.1% (60/181; 95% confidence interval [CI] 26.3-40.5%), and T. gondii DNA was found in 6.1% (11/181; 95% CI 3.1-10.6) of the diaphragm samples. We isolated the parasite from 2 of 32 goat-kids, and the T. gondii strains belonged to genotype II. The results showed that 1/3 of 3-mo-old goats may be infected with T. gondii, and their consumption during Easter (as barbecue) may seriously compromise food safety as a result.
authors.affiliation: University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Parasitology and Parasitic Diseases Department, 3-5 Mănăştur Street, cod 400372, Cluj-Napoca, Romania.
authors.forename: Anamaria I
authors.initials: AI
authors.lastname: Paştiu
authors.forename: Daniel
authors.initials: D
authors.lastname: Ajzenberg
authors.forename: Adriana
authors.initials: A
authors.lastname: Györke
authors.forename: Ovidiu
authors.initials: O
authors.lastname: Şuteu
authors.forename: Anamaria
authors.initials: A
authors.lastname: Balea
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Rosenthal
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Kalmár
authors.forename: Cristian
authors.initials: C
authors.lastname: Domşa
authors.forename: Vasile
authors.initials: V
authors.lastname: Cozma
mesh_terms: D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001681:Biological Assay / Q000662:veterinary; D002415:Cats; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D003964:Diaphragm / Q000469:parasitology; D005838:Genotype; D015511:Goat Diseases / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission; D006041:Goats; D006801:Humans; D051379:Mice; D018895:Microsatellite Repeats; D015995:Prevalence; D012383:Romania / Q000453:epidemiology; D014122:Toxoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000453:epidemiology / Q000635:transmission*; D015047:Zoonoses / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan