title: Proteome dynamics in neutrophils of adult zebrafish upon chemically-induced inflammation.
abstract: Neutrophils are the most abundant polymorphonuclear leukocytes, presenting the first line of defence against infection or tissue damage. To characterize the molecular changes on the protein level in neutrophils during sterile inflammation we established the chemically-induced inflammation (ChIn) assay in adult zebrafish and investigated the proteome dynamics within neutrophils of adult zebrafish upon inflammation. Through label-free proteomics we identified 48 proteins that were differentially regulated during inflammation. Gene ontology analysis revealed that these proteins were associated with cell cycle, nitric oxide signalling, regulation of cytoskeleton rearrangement and intermediate filaments as well as immune-related processes such as antigen presentation, leucocyte chemotaxis and IL-6 signalling. Comparison of protein expression dynamics with transcript expression dynamics suggests the existence of regulatory mechanisms confined to the protein level for some genes. This is the first proteome analysis of adult zebrafish neutrophils upon chemically-induced inflammation providing a valuable reference for future studies using zebrafish inflammation models.
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Sachin Kumar
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Max Planck Institute for Heart and Lung Research, Ludwigstr. 43, 61231 Bad Nauheim, Germany.
authors.forename: Sriram
authors.initials: S
authors.lastname: Aravamudhan
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Olivier
authors.initials: O
authors.lastname: Armant
authors.affiliation: Max Planck Institute for Heart and Lung Research, Ludwigstr. 43, 61231 Bad Nauheim, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Krüger
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), 76344 Eggenstein-Leopoldshafen, Germany. Electronic address: clemens.grabher@kit.edu.
authors.forename: Clemens
authors.initials: C
authors.lastname: Grabher
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects*; D007249:Inflammation / Q000139:chemically induced; D013058:Mass Spectrometry; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D020543:Proteome / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects*; D015027:Zebrafish / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D020543:Proteome; D029961:Zebrafish Proteins
keywords: Inflammation; Neutrophils; Proteome; Zebrafish