title: Recent H3N2 influenza virus clinical isolates rapidly acquire hemagglutinin or neuraminidase mutations when propagated for antigenic analyses.
abstract: Prior to serological testing, influenza viruses are typically propagated in eggs or cell culture. Recent human H3N2 strains bind to cells with low avidity. Here, we isolated nine primary H3N2 viral isolates from respiratory secretions of children. Upon propagation in vitro, five of these isolates acquired hemagglutinin or neuraminidase mutations that increased virus binding to cell surfaces. These mutations can potentially confound serological assays commonly used to identify antigenically novel influenza viruses.
authors.affiliation: Wistar Institute, Philadelphia, Pennsylvania, USA Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Benjamin S
authors.initials: BS
authors.lastname: Chambers
authors.affiliation: Wistar Institute, Philadelphia, Pennsylvania, USA Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Clinical Virology Laboratory, Children's Hospital of Philadelphia, and Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Hodinka
authors.affiliation: Wistar Institute, Philadelphia, Pennsylvania, USA Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA shensley@wistar.org.
authors.forename: Scott E
authors.initials: SE
authors.lastname: Hensley
mesh_terms: D000818:Animals; D000940:Antigenic Variation; D002648:Child; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000201:enzymology / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D007251:Influenza, Human / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D009439:Neuraminidase / Q000235:genetics* / Q000276:immunology; D012692:Serial Passage; D014776:Virus Cultivation
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase