title: Microfluidic cryofixation for correlative microscopy.
abstract: Cryofixation yields outstanding ultrastructural preservation of cells for electron microscopy, but current methods disrupt live cell imaging. Here we demonstrate a microfluidic approach that enables cryofixation to be performed directly in the light microscope with millisecond time resolution and at atmospheric pressure. This will provide a link between imaging/stimulation of live cells and post-fixation optical, electron, or X-ray microscopy.
authors.affiliation: Max Planck Institute for Biophysical Chemistry, 37077 Goettingen, Germany. tburg@mpibpc.mpg.de.
authors.forename: Yara X
authors.initials: YX
authors.lastname: Mejia
authors.forename: Holger
authors.initials: H
authors.lastname: Feindt
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Zhang
authors.forename: Siegfried
authors.initials: S
authors.lastname: Steltenkamp
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Burg
mesh_terms: D003080:Cold Temperature; D044085:Microfluidics; D008853:Microscopy / Q000379:methods*