title: Complete genome sequence of a broad-host-range lytic Dickeya spp. bacteriophage ϕD5.
abstract: Little is known about lytic bacteriophages infecting plant-pathogenic Dickeya spp. These bacteria cause economically significant losses in arable crops and ornamental plant production worldwide. At present, there is no effective control of diseases caused by Dickeya spp. A novel bacteriophage, ϕD5, belonging to the family Myoviridae, order Caudovirales, that could be used to control these bacteria was isolated previously. This report provides information on its characterization. The ϕD5 genome consists of 155,346-bp-length double-stranded DNA with a GC content of 49.7 % and is predicted to have 196 open reading frames (ORFs) with an average length of 711 nucleotides each. The ORFs were classified into functional groups, including phage structure, packaging, DNA metabolism, regulation, and additional functions. The phage lifestyle predicted from PHACTS indicated that ϕD5 may be a lytic phage and therefore can efficiently kill plant-pathogenic Dickeya spp.
authors.affiliation: Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk, Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland, robert.czajkowski@biotech.ug.edu.pl.
authors.forename: Robert
authors.initials: R
authors.lastname: Czajkowski
authors.forename: Zofia
authors.initials: Z
authors.lastname: Ozymko
authors.forename: Szymon
authors.initials: S
authors.lastname: Zwirowski
authors.forename: Ewa
authors.initials: E
authors.lastname: Lojkowska
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004755:Enterobacteriaceae / Q000502:physiology / Q000821:virology*; D016679:Genome, Viral; D058507:Host Specificity; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*