title: Comparison of two headspace sampling techniques for the analysis of off-flavour volatiles from oat based products.
abstract: Two different headspace sampling techniques were compared for analysis of aroma volatiles from freshly produced and aged plain oatcakes. Solid phase microextraction (SPME) using a Carboxen-Polydimethylsiloxane (PDMS) fibre and entrainment on Tenax TA within an adsorbent tube were used for collection of volatiles. The effects of variation in the sampling method were also considered using SPME. The data obtained using both techniques were processed by multivariate statistical analysis (PCA). Both techniques showed similar capacities to discriminate between the samples at different ages. Discrimination between fresh and rancid samples could be made on the basis of changes in the relative abundances of 14-15 of the constituents in the volatile profiles. A significant effect on the detection level of volatile compounds was observed when samples were crushed and analysed by SPME-GC-MS, in comparison to undisturbed product. The applicability and cost effectiveness of both methods were considered.
authors.affiliation: Enhancing Crop Productivity and Utilisation Theme, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA Scotland, UK.
authors.forename: Claudine
authors.initials: C
authors.lastname: Cognat
authors.affiliation: Enhancing Crop Productivity and Utilisation Theme, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA Scotland, UK.
authors.forename: Tom
authors.initials: T
authors.lastname: Shepherd
authors.affiliation: Enhancing Crop Productivity and Utilisation Theme, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA Scotland, UK.
authors.forename: Susan R
authors.initials: SR
authors.lastname: Verrall
authors.affiliation: Enhancing Crop Productivity and Utilisation Theme, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA Scotland, UK; School of Life Sciences, Heriot-Watt University, Edinburgh, EH14 4AS Scotland, UK.
authors.forename: Derek
authors.initials: D
authors.lastname: Stewart
mesh_terms: D018554:Avena / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D052617:Solid Phase Microextraction / Q000379:methods*