title: Collagen scaffold arrays for combinatorial screening of biophysical and biochemical regulators of cell behavior.
abstract: Arrays of 3D macroporous collagen scaffolds with orthogonal gradations of structural and biomolecular cues are described. Gradient maker technology is applied to create linear biomolecular gradients within microstructurally distinct sections of a single CG scaffold array. The array set up is used to explore cell behaviors including proliferation and regulation of stem cell fate.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 104 RAL, 600 S. Mathews Ave., Urbana, IL, 61801, USA.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Caliari
authors.forename: Emily A
authors.initials: EA
authors.lastname: Gonnerman
authors.forename: William K
authors.initials: WK
authors.lastname: Grier
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Weisgerber
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Banks
authors.forename: Aurora J
authors.initials: AJ
authors.lastname: Alsop
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Lee
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Bailey
authors.forename: Brendan A C
authors.initials: BA
authors.lastname: Harley
mesh_terms: D000818:Animals; D002460:Cell Line; D003094:Collagen / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D046888:Tissue Array Analysis / Q000295:instrumentation / Q000379:methods
chemical_list: D003094:Collagen
keywords: arrays; collagen; freeze-drying; gradients; mesenchymal stem cells