title: Estrogen and bisphenol A affect male rat enamel formation and promote ameloblast proliferation.
abstract: Bisphenol A (BPA) is a widespread endocrine disrupting chemical (EDC) strongly suspected to have adverse health effects. Numerous tissues and cells are affected by BPA, and we showed recently that BPA targets include ameloblasts and enamel. We therefore investigated the effects of BPA on ameloblasts and the possible involvement of the estrogen signaling pathway. Rats were exposed daily to low-dose BPA, and developed enamel hypomineralization similar to human molar incisor hypomineralization (MIH). BPA increased ameloblast proliferation in vivo and in vitro. The proliferation of the rat dental epithelial cell line HAT-7 was also increased by estrogen (E2). Ameloblasts express ERα but not ERβ both in vivo and in vitro. The ER antagonist ICI 182,780 was used to inactivate ERα and abolished the effects of E2 on cell proliferation and transcription, but only partially reduced the effects of BPA. In conclusion, we show, for the first time, that: 1) BPA has ER-dependent and ER-independent effects on ameloblast proliferation and gene transcription; 2) the estrogen signaling pathway is involved in tooth development and the enamel mineralization process; and 3) BPA impacts preferentially amelogenesis in male rats. These results are consistent with the steroid hormones having effect on ameloblasts, raising the issues of the hormonal influence on amelogenesis and possible differences in enamel quality between sexes.
authors.affiliation: Centre de Recherche des Cordeliers (K.J., S.L., C.M., A.B., S.B), Institut National de la Santé et de la Recherche Médicale UMRS 1138, Laboratory of Molecular Oral Pathophysiology; Université Paris-Descartes (K.J., S.L.C.M.,A.B., S.B.); Université Pierre et Marie Curie-Paris (K.J., S.L., C.M., A.B., S.B); and Université Paris-Diderot (K.J., A.B., S.B.), UFR d'Odontologie, F-75006, Paris, France; I2MC (A.V.), Institut National de la Santé et de la Recherche Médicale U1048, équipe 9 and Université Paul Sabatier (A.V.), 31432 Toulouse, France; Institut National de la Recherche Agronomique UMR 1324 (M.-C.C.L.), Centre des sciences du gout et de l'alimentation - BP 86 510; CNRS UMR 6265 (M.-C.C.L.), Centre des sciences du gout et de l'alimentation; and Université de Bourgogne (M.-C.C.L.), Centre des sciences du gout et de l'alimentation, 21 065 Dijon, France; and Centre de Référence des maladies rares de la face et de la cavité buccale MAFACE hôpital Rothschild (A.B.), AP-HP, 75012 Paris, France.
authors.forename: Katia
authors.initials: K
authors.lastname: Jedeon
authors.forename: Sophia
authors.initials: S
authors.lastname: Loiodice
authors.forename: Clémence
authors.initials: C
authors.lastname: Marciano
authors.forename: Alexia
authors.initials: A
authors.lastname: Vinel
authors.forename: Marie-Chantal
authors.initials: MC
authors.lastname: Canivenc Lavier
authors.forename: Ariane
authors.initials: A
authors.lastname: Berdal
authors.forename: Sylvie
authors.initials: S
authors.lastname: Babajko
mesh_terms: D000565:Ameloblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000566:Amelogenesis / Q000187:drug effects; D000818:Animals; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D003743:Dental Enamel / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D052244:Endocrine Disruptors / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds