title: Gellan gum microspheres containing a novel α-amylase from marine Nocardiopsis sp. strain B2 for immobilization.
abstract: A Nocardiopsis sp. stain B2 with an ability to produce stable α-amylase was isolated from marine sediments. The characterization of microorganism was done by biochemical tests and 16S rDNA sequencing. The α-amylase was purified by gel filtration chromatography by using sephadex G-75. The molecular mass of the amylase was found to be 45 kDa by SDS-PAGE and gel filtration chromatography. The isolated α-amylase was immobilized by ionotropic gelation technique using gellan gum (GG). These microspheres were spherical with average particle size of 375.62±21.76 to 492.54±32.18 μm. The entrapment efficiency of these α-amylase loaded GG microspheres was found 74.76±1.32 to 87.64±1.52%. Characterization of α-amylase-gellan gum microspheres was confirmed using FTIR and SEM analysis. The in vitro amylase release kinetic have been studied by various mathematical models that follow the Korsmeyer-Peppas model (R2=0.9804-0.9831) with anomalous (non-Fickian) diffusion release mechanism.
authors.affiliation: Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, W.B., India.
authors.forename: Samrat
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, W.B., India. Electronic address: janapharmacy@rediffmail.com.
authors.forename: Sougata
authors.initials: S
authors.lastname: Jana
authors.affiliation: Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, W.B., India.
authors.forename: Arijit
authors.initials: A
authors.lastname: Gandhi
authors.affiliation: Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, W.B., India.
authors.forename: Kalyan Kumar
authors.initials: KK
authors.lastname: Sen
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, No. 13 Beiyitiao, Zhongguancun, Beijing 100080, PR China.
authors.forename: Wang
authors.initials: W
authors.lastname: Zhiang
authors.affiliation: Department of Pharmaceutics, Sinhgad Institute of Pharmacy, Narhe, Pune 411 041, India.
authors.forename: Chandrakant
authors.initials: C
authors.lastname: Kokare
mesh_terms: D039903:Actinobacteria / Q000201:enzymology* / Q000254:growth & development; D004789:Enzyme Activation / Q000187:drug effects; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008863:Microspheres; D010316:Particle Size; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012965:Sodium Chloride / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D000516:alpha-Amylases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004800:Enzymes, Immobilized; D011135:Polysaccharides, Bacterial; D012965:Sodium Chloride; C048288:gellan gum; D000516:alpha-Amylases
keywords: Gellan gum; Immobilization; In vitro release; Nocardiopsis sp.; α-Amylase