title: Cyclin D2 plays a regulatory role in HBV replication.
abstract: Hepatitis B virus (HBV) infection is the leading cause of liver diseases. However, the molecular mechanisms of HBV infection and carcinogenesis have not been fully elucidated. In this study, we found that cyclin D2 was upregualted in HBV-expressing cells and liver tissues of HBV-transgenic mice. Gene silencing of cyclin D2 inhibited HBV DNA replicative intermediates, 3.5 kb mRNA, core protein level, as well as the secretions of HBsAg and HBeAg. On the contrary, overexpression of cyclin D2 promoted HBV replication. Furthermore, cyclin D2 regulated HBV replication by enhancing the activity of HBV core and Sp1 promoters by targeting transcription factor CREB2. Silencing of CREB2 abolished enhancement of HBV replication induced by cyclin D2. Together, our study has uncovered a positive role of cyclin D2 in HBV replication. It is conceivable that therapeutic application of cyclin D2 inhibitor in HBV infection therapy.
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Chun-li
authors.initials: CL
authors.lastname: Song
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Ji-hua
authors.initials: JH
authors.lastname: Ren
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Long-kuan
authors.initials: LK
authors.lastname: Ran
authors.affiliation: Department of Forensic Medicine, Chongqing Medical University, Chongqing, China.
authors.forename: Yong-guo
authors.initials: YG
authors.lastname: Li
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Xiao-song
authors.initials: XS
authors.lastname: Li
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Wan-yu
authors.initials: WY
authors.lastname: Li
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Ai-long
authors.initials: AL
authors.lastname: Huang
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing, China. Electronic address: yixin_xinyuan@163.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D056742:Cyclin D2 / Q000378:metabolism*; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000502:physiology*; D022781:Hepatocytes; D054884:Host-Pathogen Interactions; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D014779:Virus Replication
chemical_list: C080336:CCND2 protein, human; C486196:Ccnd2 protein, mouse; D056742:Cyclin D2
keywords: Cyclin D2; HBV promoter; HBV replication; Hepatitis B virus