title: Fluorescent probes for exploring plant cell wall deconstruction: a review.
abstract: Plant biomass is a potential resource of chemicals, new materials and biofuels that could reduce our dependency on fossil carbon, thus decreasing the greenhouse effect. However, due to its chemical and structural complexity, plant biomass is recalcitrant to green biological transformation by enzymes, preventing the establishment of integrated bio-refineries. In order to gain more knowledge in the architecture of plant cell wall to facilitate their deconstruction, many fluorescent probes bearing various fluorophores have been devised and used successfully to reveal the changes in structural motifs during plant biomass deconstruction, and the molecular interactions between enzymes and plant cell wall polymers. Fluorescent probes are thus relevant tools to explore plant cell wall deconstruction.
authors.affiliation: INRA (French National Institute for Agricultural Research), UMR0614 Fractionation of AgroResources and Environment, 2 esplanade Roland-Garros, 51100 Reims, France. gabriel.paes@reims.inra.fr.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Paës
mesh_terms: D055162:Biocatalysis; D002473:Cell Wall / Q000737:chemistry* / Q000648:ultrastructure; D044602:Cellulases / Q000737:chemistry; D003246:Conservation of Energy Resources; D036681:Fluorescence Recovery After Photobleaching; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry; D008031:Lignin / Q000737:chemistry; D059828:Plant Cells / Q000737:chemistry / Q000648:ultrastructure; D059205:Renewable Energy; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; C036909:lignocellulose; D049452:Green Fluorescent Proteins; D008031:Lignin; D044602:Cellulases