title: Value of expression of insulin-like growth factor-1 receptor in gastric adenocarcinomas and normal gastric tissues.
abstract: OBJECTIVE
  Insulin-like growth factor-1 receptor (IGF-1R) plays critical roles in cell proliferation, differentiation, apoptosis, and transformation. Suppression of IGF-1R by means of antisense methods and specific antibodies causes cell apoptosis and growth inhibition of cancer cells. The present study aims to investigate whether there is a difference between normal and cancerous tissue with respect to IGF-1R expression and to assess the relationship between IGF-1R expression and tumor stage, degree of differentiation, and lymph node metastasis by examining IGF-1R expression in cancerous and normal tissues of gastric adenocarcinoma cases of different stages.
  
  
  METHODS
  By using immunohistochemical methods, IGF-1Rb (H-60) (1/100, Santa Cruz Biotechnology, SC-9038, Texas,USA) expression was investigated in paraffin-embedded blocks obtained from total/partial gastrectomy material pertaining to 47 gastric adenocarcinoma cases. IGF-1R expression was evaluated semi-quantitatively in terms of intensity and distribution in both normal and cancerous tissues.
  
  
  RESULTS
  Insulin-like growth factor-1 receptor expression mean score was 5.38 and 8.40 for cancerous and for normal gastric tissues, respectively. IGF-1R expression decreased significantly in cancerous tissues compared normal tissue (p:0.001). When all cases with and without lymph node metastasis were analyzed, IGF-1R expression was observed to decrease for cases with lymph node metastasis compared to those without lymph node metastasis (p:0.035).
  
  
  CONCLUSIONS
  Insulin-like growth factor-1 receptor expression in gastric cancer tissue has proven to be considerably lower than IGF-1R expression in normal gastric mucosa. Metastatic progression reduces IGF-1R expression gradually in cancer tissue.
authors.affiliation: Department of Gastroenterology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey. fundaugurkantar@yahoo.com.
authors.forename: Ömer
authors.initials: Ö
authors.lastname: Topalak
authors.forename: Funda
authors.initials: F
authors.lastname: Uğur Kantar
authors.forename: Çağnur
authors.initials: Ç
authors.lastname: Ulukuş
authors.forename: Ali
authors.initials: A
authors.lastname: Küpelioğlu
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000556:secondary*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry*; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D017526:Receptor, IGF Type 1 / Q000032:analysis*; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000473:pathology
chemical_list: D017526:Receptor, IGF Type 1