title: Purification and mass spectrometry based characterization of a pediocin produced by Pediococcus acidilactici 13.
abstract: Bacteriocins are antimicrobial peptides produced by several bacterial species. Among the bacteriocins pediocin-like bacteriocins have a significant inhibitory activity on the foodborne pathogens especially on Listeria monocytogenes. This study aims to select a simple and usable purification method to purify/concentrate the antimicrobial peptide and characterization of the bacteriocin produced by Pediococcus acidilactici 13 by using proteomic approaches which is a recent omic technology. For purification dialysis, ultrafiltration method was used, and as a result of this study the bacteriocin activity reached 819,200 AU/mL from 102,400 AU/mL initially. Two dimensional gel electrophoresis and then matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) analysis were carried out to identify the current bacteriocin and related proteins. Obtained data revealed similarity to pediocin PA-1 transport/processing ATP-binding protein PedD (accession number: P36497), pediocin operon PedC (accession number: Q68GC4) and bacteriocin pediocin PA-1 (accession number: P29430) from UniProtKB/Swiss-Prot databank, thus the bacteriocin produced by P. acidilactici 13 is considered similar to pediocin PA-1.
authors.affiliation: Central Laboratory, Biotechnology Institute, Ankara University, 06110, Tandoğan, Ankara, Turkey, gunes_evrim@yahoo.com.
authors.forename: Evrim Güneş
authors.initials: EG
authors.lastname: Altuntaş
authors.forename: Kamuran
authors.initials: K
authors.lastname: Ayhan
authors.forename: Selen
authors.initials: S
authors.lastname: Peker
authors.forename: Beycan
authors.initials: B
authors.lastname: Ayhan
authors.forename: Duygu Ozel
authors.initials: DO
authors.lastname: Demiralp
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D010376:Pediococcus / Q000235:genetics / Q000378:metabolism*
chemical_list: D023181:Antimicrobial Cationic Peptides; D001430:Bacteriocins