title: Antiradical activity of gallic acid included in lipid interphases.
abstract: Polyphenols are well known as antioxidant agents and by their effects on the hydration layers of lipid interphases. Among them, gallic acid and its derivatives are able to decrease the dipole potential and to act in water as a strong antioxidant. In this work we have studied both effects on lipid interphases in monolayers and bilayers of dimyristoylphosphatidylcholine. The results show that gallic acid (GA) increases the negative surface charges of large unilamellar vesicles (LUVs) and decreases the dipole potential of the lipid interphase. As a result, positively charged radical species such as ABTS(+) are able to penetrate the membrane forming an association with GA. These results allow discussing the antiradical activity (ARA) of GA at the membrane phase which may be taking place in water spaces between the lipids.
authors.affiliation: Laboratory of Biointerphases and Biomimetic Systems, Centro de Investigación y Transferencia de Santiago del Estero (CITSE) (University of Santiago del Estero-CONICET), 4200 Santiago del Estero, Argentina; Laboratory of Antioxidants and Oxidative Processes, Centro de Investigación y Transferencia de Santiago del Estero (CITSE) (University of Santiago del Estero- CONICET), 4200 Santiago del Estero, Argentina.
authors.forename: C L
authors.initials: CL
authors.lastname: Salcedo
authors.affiliation: Laboratory of Biointerphases and Biomimetic Systems, Centro de Investigación y Transferencia de Santiago del Estero (CITSE) (University of Santiago del Estero-CONICET), 4200 Santiago del Estero, Argentina.
authors.forename: M A
authors.initials: MA
authors.lastname: Frías
authors.affiliation: Laboratory of Biointerphases and Biomimetic Systems, Centro de Investigación y Transferencia de Santiago del Estero (CITSE) (University of Santiago del Estero-CONICET), 4200 Santiago del Estero, Argentina.
authors.forename: A C
authors.initials: AC
authors.lastname: Cutro
authors.affiliation: Laboratory of Antioxidants and Oxidative Processes, Centro de Investigación y Transferencia de Santiago del Estero (CITSE) (University of Santiago del Estero- CONICET), 4200 Santiago del Estero, Argentina. Electronic address: manazar2004@yahoo.com.
authors.forename: M A
authors.initials: MA
authors.lastname: Nazareno
authors.affiliation: Laboratory of Biointerphases and Biomimetic Systems, Centro de Investigación y Transferencia de Santiago del Estero (CITSE) (University of Santiago del Estero-CONICET), 4200 Santiago del Estero, Argentina. Electronic address: disalvoanibal@yahoo.com.ar.
authors.forename: E A
authors.initials: EA
authors.lastname: Disalvo
mesh_terms: D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D005707:Gallic Acid / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D008051:Lipid Bilayers; D005707:Gallic Acid; D004134:Dimyristoylphosphatidylcholine
keywords: Antioxidant effect; Gallic acid; Lipid membrane; Surface pressure; Zeta potential