title: Antimicrobial nanospheres thin coatings prepared by advanced pulsed laser technique.
abstract: We report on the fabrication of thin coatings based on polylactic acid-chitosan-magnetite-eugenol (PLA-CS-Fe3O4@EUG) nanospheres by matrix assisted pulsed laser evaporation (MAPLE). Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) investigation proved that the homogenous Fe3O4@EUG nanoparticles have an average diameter of about 7 nm, while the PLA-CS-Fe3O4@EUG nanospheres diameter sizes range between 20 and 80 nm. These MAPLE-deposited coatings acted as bioactive nanosystems and exhibited a great antimicrobial effect by impairing the adherence and biofilm formation of Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) bacteria strains. Moreover, the obtained nano-coatings showed a good biocompatibility and facilitated the normal development of human endothelial cells. These nanosystems may be used as efficient alternatives in treating and preventing bacterial infections.
authors.affiliation: University of Bucharest, Faculty of Biology, Microbiology Department, Aleea Portocalelor no 1-3, 060101 Bucharest, Romania.
authors.forename: Alina Maria
authors.initials: AM
authors.lastname: Holban
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, 011061 Bucharest, Romania ; National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O.Box MG-36, Bucharest-Magurele, Romania.
authors.forename: Valentina
authors.initials: V
authors.lastname: Grumezescu
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, 011061 Bucharest, Romania.
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, 011061 Bucharest, Romania.
authors.forename: Bogdan Ştefan
authors.initials: BŞ
authors.lastname: Vasile
authors.affiliation: S.C. Metav-CD S.A., 31 Rosetti Str., 020015 Bucharest, Romania.
authors.forename: Roxana
authors.initials: R
authors.lastname: Truşcă
authors.affiliation: National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O.Box MG-36, Bucharest-Magurele, Romania.
authors.forename: Rodica
authors.initials: R
authors.lastname: Cristescu
authors.affiliation: National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O.Box MG-36, Bucharest-Magurele, Romania.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Socol
authors.affiliation: Flow Cytometry and Cell Therapy Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" (ICBP), Bucharest, Romania.
authors.forename: Florin
authors.initials: F
authors.lastname: Iordache
keywords: P. aeruginosa; S. aureus; antimicrobial; chitosan; magnetite nanoparticles; nanospheres; polylactic acid