title: Daily Feeding of Fructooligosaccharide or Glucomannan Delays Onset of Senescence in SAMP8 Mice.
abstract: We hypothesized that daily intake of nondigestible saccharides delays senescence onset through the improvement of intestinal microflora. Here, we raised senescence accelerated mice prone 8 (SAMP8) on the AIN93 diet (CONT), with sucrose being substituted for 5% of fructooligosaccharide (FOS) or 5% of glucomannan (GM), 15 mice per group. Ten SAMR1 were raised as reference of normal aging with control diet. Grading of senescence was conducted using the method developed by Hosokawa, and body weight, dietary intake, and drinking water intake were measured on alternate days. Following 38 weeks of these diets we evaluated learning and memory abilities using a passive avoidance apparatus and investigated effects on the intestinal microflora, measured oxidative stress markers, and inflammatory cytokines. Continuous intake of FOS and GM significantly enhanced learning and memory ability and decelerated senescence development when compared with the CONT group. Bifidobacterium levels were significantly increased in FOS and GM-fed mice. Urinary 8OHdG, 15-isoprostane, serum TNF- α , and IL-6 were also lower in FOS-fed mice, while IL-10 in FOS and GM groups was higher than in CONT group. These findings suggest that daily intake of nondigestible saccharides delays the onset of senescence via improvement of intestinal microflora.
authors.affiliation: Graduate School of Human Health Science, University of Nagasaki Siebold, 1-1-1 Manabino, Nagayo, Nagasaki 8512195, Japan ; Institute of Food, Nutrition and Health, Jumonji University, 2-1-28 Sugasawa, Niiza, Saitama 3528510, Japan.
authors.forename: Sadako
authors.identifier: 0000-0002-1009-9257
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Graduate School of Human Health Science, University of Nagasaki Siebold, 1-1-1 Manabino, Nagayo, Nagasaki 8512195, Japan ; Nagasaki City Hospital Organization, Nagasaki Municipal Hospital, 6-39 Shinchimachi, Nagasaki 8508555, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Kondo
authors.affiliation: Graduate School of Human Health Science, University of Nagasaki Siebold, 1-1-1 Manabino, Nagayo, Nagasaki 8512195, Japan.
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Yamaguchi
authors.affiliation: Kyoto Koka Women's University, 38 Kadono-cho, Nishikyogoku, Ukyo-ku, Kyoto 6150882, Japan.
authors.forename: Michiru
authors.identifier: 0000-0002-0937-4995
authors.initials: M
authors.lastname: Hashiguchi
authors.affiliation: Graduate School of Human Health Science, University of Nagasaki Siebold, 1-1-1 Manabino, Nagayo, Nagasaki 8512195, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Tanabe
authors.affiliation: Graduate School of Human Health Science, University of Nagasaki Siebold, 1-1-1 Manabino, Nagayo, Nagasaki 8512195, Japan ; Kyoto Koka Women's University, 38 Kadono-cho, Nishikyogoku, Ukyo-ku, Kyoto 6150882, Japan.
authors.forename: Chihiro
authors.identifier: 0000-0001-8132-8785
authors.initials: C
authors.lastname: Ushiroda
authors.affiliation: Graduate School of Human Health Science, University of Nagasaki Siebold, 1-1-1 Manabino, Nagayo, Nagasaki 8512195, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Kawahashi-Tokuhisa
authors.affiliation: Division of Immunology, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 8528523, Japan.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Yui
authors.affiliation: Division of Immunology, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 8528523, Japan.
authors.forename: Mana
authors.initials: M
authors.lastname: Miyakoda
authors.affiliation: Graduate School of Human Health Science, University of Nagasaki Siebold, 1-1-1 Manabino, Nagayo, Nagasaki 8512195, Japan ; Institute of Food, Nutrition and Health, Jumonji University, 2-1-28 Sugasawa, Niiza, Saitama 3528510, Japan.
authors.forename: Tsuneyuki
authors.initials: T
authors.lastname: Oku