title: Six-day selenium supplementation led to either UVA-photoprotection or toxic effects in human fibroblasts depending on the chemical form and dose of Se.
abstract: Selenium (Se) is an essential trace element with a narrow safety zone and unclear effects on skin photoageing. The aim of this work was to investigate the photoageing properties of sodium selenite or selenomethionine (SeMet) after a long term (6 days) Se supplementation in normal human skin fibroblasts (NHSF) subjected to ultraviolet-A (UVA) irradiation inducing 30% cell death. The uptake, toxicity and antioxidant effects of sodium selenite and SeMet were compared to better understand their photoageing properties. SeMet uptake was better than sodium selenite and their uptake by fibroblasts was not via an actively transport process. Sodium selenite induced a higher toxicity than SeMet. At 5 μM, sodium selenite inhibited cell proliferation associated with a blockage in the G2 phase and induced DNA fragmentation leading to caspase-3-dependent apoptosis cell death. At low doses (<1 μM), SeMet and sodium selenite induced glutathione peroxidase-1 (GPX1) activity and selenoproteinW1 (SEPW1) transcript expression but metalloproteinase (MMP)-1 was only induced by sodium selenite. SeMet and sodium selenite did not protect NHSFs from UVA-induced cell death. However, SeMet decreased malondialdehyde (MDA) and protected NHSFs from UVA-induced MMP1 and MMP3. We then observed a large difference in terms of photoprotection according to selenium forms. SeMet may be a potential agent for the prevention and treatment of skin photoageing.
authors.affiliation: Unité de Biochimie Hormonale et Nutritionnelle, Département de Biochimie, Toxicologie et Pharmacologie, Institut de Biologie et de Pathologie, Centre Hospitalier Universitaire de Grenoble, CS 10217, 38043 Grenoble, France. FPuch@chu-grenoble.fr.
authors.forename: Florence
authors.initials: F
authors.lastname: Hazane-Puch
authors.forename: Pierre
authors.initials: P
authors.lastname: Champelovier
authors.forename: Josiane
authors.initials: J
authors.lastname: Arnaud
authors.forename: Candice
authors.initials: C
authors.lastname: Trocmé
authors.forename: Catherine
authors.initials: C
authors.lastname: Garrel
authors.forename: Patrice
authors.initials: P
authors.lastname: Faure
authors.forename: François
authors.initials: F
authors.lastname: Laporte
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects / Q000528:radiation effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012643:Selenium / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity*; D012645:Selenomethionine / Q000494:pharmacology / Q000633:toxicity; D012867:Skin / Q000166:cytology*; D018038:Sodium Selenite / Q000494:pharmacology / Q000633:toxicity; D013054:Spectrophotometry, Atomic; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D012645:Selenomethionine; D012643:Selenium; D018038:Sodium Selenite