title: Repeated use of stable magnetic flocculant for efficient harvest of oleaginous Chlorella sp.
abstract: In the present study, a simple magnetic-particle recycling strategy was developed for harvest of the oleaginous microalga Chlorella sp. KR-1. The method entails the flocculation of microalgal cells and bare-Fe3O4 magnetic particles (bMP) by electrostatic attraction and the subsequent recovery of the bMP from the harvested flocs by electrostatic repulsion below and above the isoelectric points (IEP), respectively. For 10 recycles, the bMP showed 94-99% and 90-97% harvest and recovery efficiencies, respectively. Furthermore, neither the use of bMP nor pH adjustment showed any adverse effect on the microalgal cell growth or the co-existing bacterial species, as confirmed from the subsequent medium-recycling test and denaturing gradient gel electrophoresis (DGGE) analysis.
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors.forename: Kyubock
authors.initials: K
authors.lastname: Lee
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea; Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: So Yeun
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Praveenkumar
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea; Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Bohwa
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Jung Yoon
authors.initials: JY
authors.lastname: Seo
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors.forename: Sang Goo
authors.initials: SG
authors.lastname: Jeon
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors.forename: Jeong-Geol
authors.initials: JG
authors.lastname: Na
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Dong-Myung
authors.initials: DM
authors.lastname: Kim
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea. Electronic address: ykoh@kier.re.kr.
authors.forename: You-Kwan
authors.initials: YK
authors.lastname: Oh
mesh_terms: D001419:Bacteria / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods*; D002708:Chlorella / Q000254:growth & development*; D003470:Culture Media; D058645:Denaturing Gradient Gel Electrophoresis; D005427:Flocculation; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry*; D060328:Magnetic Phenomena; D058086:Microalgae / Q000254:growth & development; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D059027:Recycling; D055672:Static Electricity
chemical_list: D003470:Culture Media; D008055:Lipids; D012336:RNA, Ribosomal, 16S
keywords: Electrostatic interaction; Harvest; Magnetic particles; Medium recycling; Microalgae