title: Controlling the complexation of polysaccharides into multi-functional colloidal assemblies for nanomedicine.
abstract: The controlled assembly of oppositely charged polysaccharides led to colloids stable in physiological media, capable of encapsulating a molecular drug and of sorbing proteins at their interface. Two types of particles were obtained: both chitosan-dextran sulfate (CS-DS) and chitosan-heparin (CS-HP) stable over 30 days in PBS at 25 and 37°C. At gastric pH 1.2, these particles remained stable over 3 days, enough for a stomach transit. The structural analysis by small angle X-ray scattering (SAXS) showed that CS-DS surface was semi-rough and chains inside particle exhibited rod-like conformation. Moreover, the particle interfaces could efficiently be functionalized with anti-OVA or anti-α4β7 antibodies, in PBS, with the conservation of the antibody bioactivity over at least 8 days. Finally, during the assembly process, a molecular model drug, AMP, could be encapsulated with a loading efficiency up to 72% for CS-DS particles and 66% for CS-HP. All these data establish that the controlled assembly process under equilibrium conditions lead to colloids well suited for the targeted nanodelivery of drugs.
authors.affiliation: Ingénierie des Matériaux Polymères, Université de Lyon, Université Lyon 1, UMR CNRS 5223 IMP, 15, Bd, A. Latarjet, Bât. Polytech, 69622 Villeurbanne Cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Costalat
authors.affiliation: Ingénierie des Matériaux Polymères, Université de Lyon, Université Lyon 1, UMR CNRS 5223 IMP, 15, Bd, A. Latarjet, Bât. Polytech, 69622 Villeurbanne Cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Alcouffe
authors.affiliation: Ingénierie des Matériaux Polymères, Université de Lyon, Université Lyon 1, UMR CNRS 5223 IMP, 15, Bd, A. Latarjet, Bât. Polytech, 69622 Villeurbanne Cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: David
authors.affiliation: Ingénierie des Matériaux Polymères, Université de Lyon, Université Lyon 1, UMR CNRS 5223 IMP, 15, Bd, A. Latarjet, Bât. Polytech, 69622 Villeurbanne Cedex, France. Electronic address: Thierry.Delair@univ-lyon1.fr.
authors.forename: T
authors.initials: T
authors.lastname: Delair
mesh_terms: D000249:Adenosine Monophosphate / Q000737:chemistry / Q000493:pharmacokinetics; D000906:Antibodies / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D016264:Dextran Sulfate / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics; D006493:Heparin / Q000737:chemistry*; D050997:Nanomedicine; D010047:Ovalbumin / Q000737:chemistry
chemical_list: D000906:Antibodies; D004337:Drug Carriers; D000249:Adenosine Monophosphate; D006493:Heparin; D010047:Ovalbumin; D048271:Chitosan; D016264:Dextran Sulfate
keywords: Antibodies; Chitosan; Colloids; Dextran sulfate; Encapsulation; Functionalization; Heparin; Polyelectrolyte complexes