title: Modeling the interaction of interferon α-1b to bovine serum albumin as a drug delivery system.
abstract: Protein-based nanoparticles represent a promising approach to carry polypeptide and protein drugs. Using both theory and experimentation, an interferon α-1b (IFN) delivery system carried by bovine serum albumin (BSA) nanoparticles was designed. Theoretical results indicate the most probable binding site and interaction mechanism for IFN on BSA. IFN has a higher binding affinity with BSA compared with small chemical drugs. The drug loading is about 8 mg/g, significantly higher than those reported in other literature. The release profiles differ between the nanoparticles prepared by the incorporation method and the adsorption method. The adsorption of IFN on BSA nanoparticles is monolayer adsorption. The fact that IFN was carried successfully by BSA nanoparticles establishes a solid basis for expanding the drug loading field of BSA nanoparticles to proteins and polypeptides.
authors.affiliation: Soft Matter Research Center and Department of Chemistry, Zhejiang University , Hangzhou, Zhejiang 310027, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Luo
authors.forename: Yihui
authors.initials: Y
authors.lastname: Wang
authors.forename: Hongge
authors.initials: H
authors.lastname: Yang
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ding
authors.forename: Haifeng
authors.initials: H
authors.lastname: Xu
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Xie
mesh_terms: D000327:Adsorption; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004337:Drug Carriers / Q000737:chemistry*; D016898:Interferon-alpha / Q000737:chemistry*; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D004337:Drug Carriers; D016898:Interferon-alpha; D012710:Serum Albumin, Bovine