title: Detailed study of the interaction between human herpesvirus 6B glycoprotein complex and its cellular receptor, human CD134.
abstract: Recently, we identified a novel receptor, CD134, which interacts with the human herpesvirus 6B (HHV-6B) glycoprotein (g)H/gL/gQ1/gQ2 complex and plays a key role in the entry of HHV-6B into target cells. However, details of the interaction between the HHV-6B gH/gL/gQ1/gQ2 complex and CD134 were unknown. In this study, we identified a cysteine-rich domain (CRD), CDR2, of CD134 that is critical for binding to the HHV-6B glycoprotein complex and HHV-6B infection. Furthermore, we found that the expression of HHV-6B gQ1 and gQ2 subunits was sufficient for CD134 binding, which is different from the binding of human herpesvirus 6A (HHV-6A) to its receptor, CD46. Finally, we identified a region in gQ1 critical for HHV-6B gQ1 function. These results contribute much to our understanding of the interaction between this ligand and receptor.
  
  
  OBJECTIVE
  We identified the domain in HHV-6B entry receptor CD134 and the components in the HHV-6B gH/gL/gQ1/gQ2 complex required for ligand-receptor binding during HHV-6B infection. Furthermore, we identified domains in gQ1 proteins of HHV-6A and -6B and a key amino acid residue in HHV-6B gQ1 required for its function. These data should be the basis for further investigation of ligand-receptor interaction in the study of HHV-6A and -6B.
authors.affiliation: Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Huamin
authors.initials: H
authors.lastname: Tang
authors.affiliation: Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Junjie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Nora F
authors.initials: NF
authors.lastname: Mahmoud
authors.affiliation: Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, Japan ymori@med.kobe-u.ac.jp.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Mori
mesh_terms: D000595:Amino Acid Sequence; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015654:Herpesvirus 6, Human / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D053262:Receptors, OX40 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019349:Roseolovirus Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D053262:Receptors, OX40; D014759:Viral Envelope Proteins