title: Regulation of extracellular oxidoreduction potential enhanced (R,R)-2,3-butanediol production by Paenibacillus polymyxa CJX518.
abstract: Cellular redox status and oxygen availability influence the product formation. Herein, decreasing agitation speed or adding vitamin C (Vc) achieved the 2,3-BDL yield of 0.40 g g(-1) or 0.39 g g(-1)glucose under batch fermentation, respectively. To our knowledge, this is the highest 2,3-BDL yield reported so far for Paenibacillus polymyxa without adding acetic acid. The NADH/NAD(+) ratio and 2,3-BDL titer could be increased significantly by reducing the agitation speed or adding Vc, indicating that the enhancement of 2,3-BDL is closely associated with the adjustment of NADH/NAD(+) ratio. Especially, Vc addition elevated the 2,3-BDL titer from 43.66 g L(-1) to 71.71 g L(-1) within 54 h under fed-batch fermentation. This is the highest titer of 2,3-BDL so far reported for P. polymyxa from glucose fermentation. This work provides a new strategy to improve 2,3-BDL production and helps us to understand the responses of P. polymyxa to extracellular oxidoreduction potential.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, People's Republic of China.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Dai
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, People's Republic of China. Electronic address: jscheng@tju.edu.cn.
authors.forename: Jing-Sheng
authors.initials: JS
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Ying-Quan
authors.initials: YQ
authors.lastname: Liang
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, People's Republic of China.
authors.forename: Tong
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, People's Republic of China.
authors.forename: Ying-Jin
authors.initials: YJ
authors.lastname: Yuan
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology; D061249:Batch Cell Culture Techniques; D001894:Borohydrides / Q000494:pharmacology; D002072:Butylene Glycols / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D042541:Intracellular Space / Q000378:metabolism; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010100:Oxygen / Q000494:pharmacology; D056507:Paenibacillus / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
chemical_list: D001894:Borohydrides; D002072:Butylene Glycols; D009243:NAD; C026978:2,3-butylene glycol; C025364:sodium borohydride; D001205:Ascorbic Acid; D010100:Oxygen
keywords: (R,R)-2,3-butanediol; NADH/NAD(+) ratio; Paenibacillus polymyxa; Redox regulation; Vitamin C