title: Growth on poly(L-lactic acid) porous scaffold preserves CD73 and CD90 immunophenotype markers of rat bone marrow mesenchymal stromal cells.
abstract: Few data are available on the effect of biomaterials on surface antigens of mammalian bone marrow-derived, adult mesenchymal stromal cells (MSCs). Since poly(L-lactic acid) or PLLA is largely used in tissue engineering of human bones, and we are developing a reverse engineering program to prototype with biomaterials the vascular architecture of bones for their bioartificial reconstruction, both in humans and animal models, we have studied the effect of porous, flat and smooth PLLA scaffolds on the immunophenotype of in vitro grown, rat MSCs in the absence of any coating, co-polymeric enrichment, and differentiation stimuli. Similar to controls on plastic, we show that our PLLA scaffold does not modify the distribution of some surface markers in rat MSCs. In particular, the maintained expression of CD73 and CD90 on two different subpopulations (small and large cells) is consistent with their adhesion to the PLLA scaffold through specialized appendages, and to their prominent content in actin. In addition, our PLLA scaffold favours retention of the intermediate filament desmin, believed a putative marker of undifferentiated state. Finally, it preserves all rat MSCs morphotypes, and allows for their survival, adhesion to the substrate, and replication. Remarkably, a subpopulation of rat MSCs grown on our PLLA scaffold exhibited formation of membrane protrusions of uncertain significance, although in a size range and morphology compatible with either motility blebs or shedding vesicles. In summary, our PLLA scaffold has no detrimental effect on a number of features of rat MSCs, primarily the expression of CD73 and CD90.
authors.affiliation: Department of Biomedical, Biotechnological and Translational Sciences (S.Bi.Bi.T) - Laboratory of Regenerative Morphology and Bioartificial Structures/S.Bi.Bi.T. Museum - Section of Human Anatomy, University of Parma, Parma, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Zamparelli
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Zini
authors.forename: Luca
authors.initials: L
authors.lastname: Cattini
authors.forename: Giulia
authors.initials: G
authors.lastname: Spaletta
authors.forename: Davide
authors.initials: D
authors.lastname: Dallatana
authors.forename: Elena
authors.initials: E
authors.lastname: Bassi
authors.forename: Fulvio
authors.initials: F
authors.lastname: Barbaro
authors.forename: Michele
authors.initials: M
authors.lastname: Iafisco
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mosca
authors.forename: Annapaola
authors.initials: A
authors.lastname: Parrilli
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
authors.forename: Roberto
authors.initials: R
authors.lastname: Giardino
authors.forename: Monica
authors.initials: M
authors.lastname: Sandri
authors.forename: Simone
authors.initials: S
authors.lastname: Sprio
authors.forename: Anna
authors.initials: A
authors.lastname: Tampieri
authors.forename: Nadir M
authors.initials: NM
authors.lastname: Maraldi
authors.forename: Roberto
authors.initials: R
authors.lastname: Toni
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D016130:Immunophenotyping; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D018800:Thy-1 Antigens / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D015415:Biomarkers; D011091:Polyesters; D011108:Polymers; D018800:Thy-1 Antigens; D019344:Lactic Acid; C033616:poly(lactide); D015720:5'-Nucleotidase