title: Probiotic lactic acid bacteria detoxify N-nitrosodimethylamine.
abstract: Humans can be exposed to N-nitroso compounds (NOCs) due to many environmental sources, as well as endogenous formation. The main nitrosamine found in food products and also synthesised in vivo by intestinal microbiota is N-nitrosodimethylamine (NDMA). It can cause cancer of the stomach, kidney and colon. The effect of four probiotic Lactobacillus strains on NDMA was studied under different culture conditions (24 h in MRS, 168 h in modified MRS N, and 168 h in phosphate buffer). HPLC and GC-TEA methods were used for NDMA determination in supernatants. The influence of lactic acid bacteria on NDMA genotoxicity was investigated by means of the comet assay. Additionally, the effect of NDMA (2-100 µg ml⁻¹) on the growth and survival of the probiotic strains was studied. The results indicate that the bacteria decreased NDMA concentration by up to 50%, depending on the culture conditions, time of incubation, NDMA concentration, pH and bacterial strain. Lb. brevis 0945 lowered the concentration and genotoxicity of NDMA most effectively by up to 50%. This could be due to either adsorption or metabolism. The growth and survival of the bacteria was not affected by any of the tested NDMA concentrations.
authors.affiliation: a Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences , Lodz University of Technology , Lodz , Poland.
authors.forename: Adriana
authors.initials: A
authors.lastname: Nowak
authors.forename: Sławomir
authors.initials: S
authors.lastname: Kuberski
authors.forename: Zdzisława
authors.initials: Z
authors.lastname: Libudzisz
mesh_terms: D000327:Adsorption; D002274:Carcinogens, Environmental / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D020552:Comet Assay; D004249:DNA Damage; D004128:Dimethylnitrosamine / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D005506:Food Contamination; D018922:HL-60 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008658:Inactivation, Metabolic; D007700:Kinetics; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D052196:Levilactobacillus brevis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D052201:Lacticaseibacillus rhamnosus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects; D009153:Mutagens / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D019936:Probiotics / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D002274:Carcinogens, Environmental; D009153:Mutagens; D004128:Dimethylnitrosamine
keywords: Lactobacillus; N-nitrosodimethylamine; detoxification; probiotics