title: Immune enhancement of Taishan Robinia pseudoacacia polysaccharide on recombinant Proteus mirabilis OmpA in chickens.
abstract: This study was conducted to evaluate the effects of Taishan Robinia pseudoacacia polysaccharide (TRPPS) on immune responses of chickens immunized with Proteus mirabilis outer membrane protein A (OmpA) recombinant protein vaccine. OmpA was expressed in Pichia pastoris and mixed with TRPPS. 360 chickens were randomly divided into six groups. Groups I to IV were treated with OmpA which contained TRPPS of three different dosages, Freund's adjuvant, respectively. Groups V and VI were treated with pure OmpA and physiological saline, respectively. The data showed that the antibody titers against OmpA, the concentration of IL-2, CD4 +, and CD8 +, T lymphocyte proliferation rate in Group II were significantly higher (P < 0.05) than those in the other groups, little difference in SIgA content was observed among groups I to VI. These results indicated that TRPPS strengthened humoral and cellular immune responses against recombinant OmpA vaccine. Moreover, 200 mg/mL TRPPS showed significance (P < 0.05) compared with Freund's adjuvant. Therefore, TRPPS can be developed into an adjuvant for recombinant subunit vaccine.
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Yongbing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Shifa
authors.initials: S
authors.lastname: Yang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China.
authors.forename: Fujie
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Avenue, Taian, Shandong 271018, PR China. Electronic address: zhangyongbing86@163.com.
authors.forename: Ruiliang
authors.initials: R
authors.lastname: Zhu
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology*; D002681:China; D005260:Female; D007114:Immunization; D007070:Immunoglobulin A / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D010843:Pichia / Q000235:genetics*; D011134:Polysaccharides / Q000276:immunology*; D011512:Proteus Infections / Q000276:immunology* / Q000517:prevention & control; D011513:Proteus mirabilis / Q000235:genetics / Q000276:immunology*; D031301:Robinia / Q000276:immunology*; D014614:Vaccines, Synthetic
chemical_list: D000276:Adjuvants, Immunologic; D001425:Bacterial Outer Membrane Proteins; D007070:Immunoglobulin A; D007376:Interleukin-2; D011134:Polysaccharides; D014614:Vaccines, Synthetic; C078167:OMPA outer membrane proteins
keywords: Adjuvant; Immune response; Proteus mirabilis; Recombinant subunit vaccine; Robinia pseudoacacia polysaccharide