title: [Plasma sB7-H3 serves as an assistant diagnostic marker for hepatitis B virus-associated hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the relationship between the expression of soluble B7-H3 (sB7-H3) in the plasma and hepatitis virus B-associated hepatocellular carcinoma (HBV-HCC).
  
  
  METHODS
  Twenty-three patients with HBV-HCC and 15 healthy donors were enrolled in this study. The level of sB7-H3 in the plasma was detected by ELISA. The expression of sB7-H3 mRNA in peripheral blood monocytes (PBMCs), tumor tissues and peri-tumor normal tissues were determined by relative real-time quantitative PCR (qRT-PCR). The level of membrane B7-H3 (mB7-H3) expressed in tumor tissues and peri-tumor normal tissues were detected by immunohistochemical staining.
  
  
  RESULTS
  The level of plasma sB7-H3 in the HBV-HCC patients was significantly higher than that in the healthy donors (P<0.05). However, the level of sB7-H3 mRNA in PBMCs was not significantly different between the HBV-HCC patients and healthy donors (P>0.05). The level of plasma sB7-H3 dropped significantly after the resection of liver tumors (P<0.05). The qRT-PCR showed the expression of sB7-H3 mRNA in tumor tissues and peri-tumor normal tissues were about 60 and 1 800 times higher than those in PBMCs of the corresponding HBV-HCC patients, respectively, and the expression of sB7-H3 mRNA in peri-tumor normal tissues was about 30 times higher than that in tumor tissues. Immunohistochemical staining revealed that the expression of mB7-H3 in peri-tumor normal tissues was higher than that in tumor tissues.
  
  
  CONCLUSIONS
  sB7-H3 in the plasma of HBV-HCC patients is mainly generated from tumor and peri-tumor normal tissues, and it can serve as an assistant diagnostic marker for HBV-HCC.
authors.affiliation: Treatment and Research Center for Infectious Diseases, No.302 Hospital of PLA, Beijing 100039, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.affiliation: Treatment and Research Center for Infectious Diseases, No.302 Hospital of PLA, Beijing 100039, China.
authors.forename: Fengyi
authors.initials: F
authors.lastname: Li
authors.affiliation: Treatment and Research Center for Infectious Diseases, No.302 Hospital of PLA, Beijing 100039, China.
authors.forename: Lingxiang
authors.initials: L
authors.lastname: Yu
authors.affiliation: Treatment and Research Center for Infectious Diseases, No.302 Hospital of PLA, Beijing 100039, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Treatment and Research Center for Infectious Diseases, No.302 Hospital of PLA, Beijing 100039, China.
authors.forename: Xu
authors.initials: X
authors.lastname: He
authors.affiliation: Treatment and Research Center for Infectious Diseases, No.302 Hospital of PLA, Beijing 100039, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Fan
authors.affiliation: Treatment and Research Center for Infectious Diseases, No.302 Hospital of PLA, Beijing 100039, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000328:Adult; D060887:B7 Antigens / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000235:genetics* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006509:Hepatitis B / Q000097:blood / Q000235:genetics* / Q000821:virology; D006515:Hepatitis B virus / Q000502:physiology; D054884:Host-Pathogen Interactions; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000821:virology; D008113:Liver Neoplasms / Q000097:blood / Q000235:genetics* / Q000821:virology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D060887:B7 Antigens; D014408:Biomarkers, Tumor; C423237:CD276 protein, human; D012333:RNA, Messenger