title: Persistent toenail onycholysis associated with Beta-papillomavirus infection of the nail bed.
abstract: Onycholysis, separation of the nail plate from the nail bed, is etiologically classified as primary (idiopathic) or secondary (eg, caused by psoriasis, squamous cell carcinoma). Repetitive microtrauma plays a role in idiopathic onycholysis and also facilitates human papillomavirus (HPV) infection. Herein, we report a case of persistent primary onycholysis associated with repetitive trauma and infection by a multiplicity of Beta-papillomavirus (Beta-PV) genotypes. An otherwise healthy 27-year-old woman presented with a 6-year history of onycholysis of the halluces and right second toe. Her occupation required wearing steel-toed boots. Fungal cultures were negative and antifungal therapy was ineffective. Punch biopsy of the hallux nail bed revealed epidermal hyperplasia, acanthosis, hypergranulosis, hyperkeratosis, and regions of koilocytosis without significant inflammation. This histopathology implicated chronic irritation and HPV infection. Immunohistochemistry demonstrated productive HPV infection. Nested PCR using degenerate consensus primers revealed infection with 5 known and 1 novel Beta-PV genotypes (HPV 5, HPV 8, HPV 20, HPV 23, HPV 37, and FA25). The histopathology of primary onycholysis is unknown. Based on the aforementioned, we propose that repetitive microtrauma caused by wearing steel-toed boots promoted onycholysis and HPV infection, the latter of which, altered the differentiation of nail bed epithelium, preventing adhesion of nail plate to the nail bed. Lastly, the presence of oncogenic Beta-PV genotypes (ie, HPV 5, 8, and 20) implicates a risk for subungual squamous cell carcinoma, particularly if the nail remains symptomatic and persistently irritated.
authors.forename: Nicole
authors.initials: N
authors.lastname: Umanoff
authors.forename: Betina
authors.initials: B
authors.lastname: Werner
authors.forename: Peter L
authors.initials: PL
authors.lastname: Rady
authors.forename: Stephen
authors.initials: S
authors.lastname: Tyring
authors.forename: J Andrew
authors.initials: JA
authors.lastname: Carlson
mesh_terms: D000328:Adult; D052686:Betapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D001706:Biopsy; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D061809:Human Papillomavirus DNA Tests; D006801:Humans; D007150:Immunohistochemistry; D009262:Nails / Q000293:injuries / Q000473:pathology / Q000821:virology*; D054039:Onycholysis / Q000150:complications* / Q000175:diagnosis / Q000209:etiology; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000821:virology*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral