title: Pseudomonas cremoricolorata strain ND07 produces N-acyl homoserine lactones as quorum sensing molecules.
abstract: Quorum sensing (QS) is a bacterial cell-to-cell communication system controlling QS-mediated genes which is synchronized with the population density. The regulation of specific gene activity is dependent on the signaling molecules produced, namely N-acyl homoserine lactones (AHLs). We report here the identification and characterization of AHLs produced by bacterial strain ND07 isolated from a Malaysian fresh water sample. Molecular identification showed that strain ND07 is clustered closely to Pseudomonas cremoricolorata. Spent culture supernatant extract of P. cremoricolorata strain ND07 activated the AHL biosensor Chromobacterium violaceum CV026. Using high resolution triple quadrupole liquid chromatography-mass spectrometry, it was confirmed that P. cremoricolorata strain ND07 produced N-octanoyl-L-homoserine lactone (C8-HSL) and N-decanoyl-L-homoserine lactone (C10-HSL). To the best of our knowledge, this is the first documentation on the production of C10-HSL in P. cremoricolorata strain ND07.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. ninayusrina@hotmail.com.
authors.forename: Nina Yusrina Muhamad
authors.initials: NY
authors.lastname: Yunos
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. tmarilyn36@gmail.com.
authors.forename: Wen-Si
authors.initials: WS
authors.lastname: Tan
authors.affiliation: Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore. chonglek.koh@nie.edu.sg.
authors.forename: Chong-Lek
authors.initials: CL
authors.lastname: Koh
authors.affiliation: Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore. choonkook.sam@nie.edu.sg.
authors.forename: Choon-Kook
authors.initials: CK
authors.lastname: Sam
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. zetty_mohamad@yahoo.com.
authors.forename: Nur Izzati
authors.initials: NI
authors.lastname: Mohamad
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. acelinetan38@yahoo.com.
authors.forename: Pui-Wan
authors.initials: PW
authors.lastname: Tan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. adrian_tan_1991@yahoo.com.
authors.forename: Tan-Guan-Sheng
authors.initials: TG
authors.lastname: Adrian
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. yinwaifong@yahoo.com.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. kokgan@um.edu.my.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D002450:Cell Communication / Q000502:physiology*; D011549:Pseudomonas / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology*; D053038:Quorum Sensing / Q000502:physiology*; D013045:Species Specificity
chemical_list: D054742:Acyl-Butyrolactones