title: Mapping-by-sequencing identifies HvPHYTOCHROME C as a candidate gene for the early maturity 5 locus modulating the circadian clock and photoperiodic flowering in barley.
abstract: Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (Hordeum vulgare L.). We fine mapped the gene using a mapping-by-sequencing approach applied on the whole-exome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5) introgression line. We demonstrate that eam5 disrupts circadian expression of clock genes. Moreover, it interacts with the major photoperiod response gene Ppd-H1 to accelerate flowering under noninductive short days. Our results suggest that HvPHYC participates in transmission of light signals to the circadian clock and thus modulates light-dependent processes such as photoperiodic regulation of flowering.
authors.affiliation: Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany.
authors.forename: Artem
authors.initials: A
authors.lastname: Pankin
authors.affiliation: Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany.
authors.forename: Chiara
authors.initials: C
authors.lastname: Campoli
authors.affiliation: Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany.
authors.forename: Xue
authors.initials: X
authors.lastname: Dong
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Seeland (OT Gatersleben), Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Kilian
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Seeland (OT Gatersleben), Germany.
authors.forename: Rajiv
authors.initials: R
authors.lastname: Sharma
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Seeland (OT Gatersleben), Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Himmelbach
authors.affiliation: Department of Crystallography, A. Mickiewicz University, 60-780 Poznan, Poland.
authors.forename: Reena
authors.initials: R
authors.lastname: Saini
authors.affiliation: Department of Crystallography, A. Mickiewicz University, 60-780 Poznan, Poland Department of Biology, University of York, YO10 5DD York, United Kingdom.
authors.forename: Seth J
authors.initials: SJ
authors.lastname: Davis
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Seeland (OT Gatersleben), Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Stein
authors.affiliation: Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany.
authors.forename: Korbinian
authors.initials: K
authors.lastname: Schneeberger
authors.affiliation: Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany Institute of Plant Genetics, Heinrich Heine University, 40225 Düsseldorf, Germany Cluster of Excellence on Plant Sciences "From Complex Traits Towards Synthetic Modules" 40225 Düsseldorf, Germany korff@mpipz.mpg.de.
authors.forename: Maria
authors.initials: M
authors.lastname: von Korff
mesh_terms: D057906:Circadian Clocks / Q000235:genetics*; D059472:Exome; D035264:Flowers / Q000235:genetics* / Q000254:growth & development; D017343:Genes, Plant; D001467:Hordeum / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D007178:Inbreeding; D017440:Photoperiod; D020161:Physical Chromosome Mapping; D010834:Phytochrome / Q000235:genetics*; D063245:Plant Development / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D040641:Quantitative Trait Loci
chemical_list: D010940:Plant Proteins; D010834:Phytochrome
keywords: barley; circadian clock; flowering; photoperiod; phytochrome