title: Genetic variants in desaturase gene, erythrocyte fatty acids, and risk for type 2 diabetes in Chinese Hans.
abstract: OBJECTIVE
  The aim of this study was to examine the association of the genetic variants in the fatty acid desaturase (FADS) gene cluster with erythrocyte phospholipid fatty acids (PLFA), and their relation to risk for type 2 diabetes mellitus (T2DM) in Han Chinese.
  
  
  METHODS
  Seven hundred and fifty-eight patients with T2DM and 400 healthy individuals were recruited. The erythrocyte PLFA and single-nucleotide polymorphism were determined by standard method.
  
  
  RESULTS
  Minor allele homozygotes and heterozygotes of rs174575 and rs174537 had lower PL 20:4 ω-6 levels in healthy individuals. Minor allele homozygotes and heterozygotes of rs174455 in FADS3 gene had lower levels of 22:5 ω-3, 20:4 ω-6, and Δ5desaturase activity in patients with T2DM. Erythrocyte membrane PL 18:3 ω-3 (P for trend = 0.002), 22:5 ω-3 (P for trend < 0.001), ω-3 polyunsaturated fatty acid (P for trend < 0.001), and ω-3:ω-6 (P for trend < 0.001) were significantly inversely associated with risk for T2DM.
  
  
  CONCLUSIONS
  Genetic variants in the FADS gene cluster are associated with altered erythrocyte PLFAs. High levels of PL 18:3 ω-3, 22:5 ω-3, and total ω-3 polyunsaturated fatty acid were associated with low risk for T2DM.
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou, China; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.affiliation: Clinical Nutrition Center, Huadong Hospital, Fudan University, Shanghai, China.
authors.forename: Jianqin
authors.initials: J
authors.lastname: Sun
authors.affiliation: Clinical Nutrition Center, Huadong Hospital, Fudan University, Shanghai, China.
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Clinical Nutrition Center, Huadong Hospital, Fudan University, Shanghai, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.affiliation: Clinical Nutrition Center, Huadong Hospital, Fudan University, Shanghai, China.
authors.forename: Danfeng
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Biostatistics, Harvard School of Public Health, Boston, MA, USA.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou, China. Electronic address: duoli@zju.edu.cn.
authors.forename: Duo
authors.initials: D
authors.lastname: Li
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D000090943:Delta-5 Fatty Acid Desaturase; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D004912:Erythrocytes / Q000378:metabolism; D005006:Ethnicity / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000097:blood*; D043371:Fatty Acids, Omega-6 / Q000097:blood; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D000090943:Delta-5 Fatty Acid Desaturase; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D010743:Phospholipids; D044943:Fatty Acid Desaturases; C533241:FADS2 protein, human; C545859:FADS3 protein, human
keywords: Fatty acid desaturase; Phospholipids; Single-nucleotide polymorphism; Type 2 diabetes