title: Interactions of eosinophils with nerves.
abstract: Coculture of eosinophils and nerves is a powerful tool in determining the interactions between the two cell types. We have developed methods for culture of parasympathetic ganglia and dorsal root ganglia from humans, and we have further refined the technique to coculture with eosinophils. Here we describe methods for coculturing primary parasympathetic ganglia or dorsal root ganglia with eosinophils.
authors.affiliation: Pulmonary and Critical Care Medicine, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, L334, 97239-3098, Portland, OR, USA, rothcart@ohsu.edu.
authors.forename: Quinn R
authors.initials: QR
authors.lastname: Roth-Carter
authors.forename: David B
authors.initials: DB
authors.lastname: Jacoby
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Nie
mesh_terms: D002450:Cell Communication / Q000502:physiology; D004804:Eosinophils / Q000166:cytology*; D005726:Ganglia, Parasympathetic / Q000166:cytology*; D005727:Ganglia, Spinal / Q000166:cytology*; D006801:Humans