title: Inhibition of BKCa channel currents in vascular smooth muscle cells contributes to HBOC-induced vasoconstriction.
abstract: The exact mechanism of hemoglobin-based oxygen carrier (HBOC)-related vasoactivity is still unclear. This study measured the isometric tension of dog arteries and large conductance Ca(2+)-activated K(+) (BKCa) channel currents in vascular smooth muscle cells after exposure to HBOC with increasing concentrations. Data indicated that the net tensions of arteries were dramatically elevated and this elevation was more prominent in coronary artery. Moreover, HBOC exhibited inhibitory effect on BKCa channel, which is strongly correlated with changes in vascular tension. Collectively, HBOC-induced vasoconstriction in a dose-dependent manner and inhibition of BKCa channel is at least partially contributing to this effect.
authors.affiliation: a Department of Burn and Plastic Surgery , West China Hospital, Sichuan University , Chengdu , P. R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: b Laboratory of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University , Chengdu , Sichuan , P. R. China.
authors.forename: Nanfu
authors.initials: N
authors.lastname: Luo
authors.affiliation: c Institute of Blood Transfusion, Chinese Academy of Medical Sciences , Chengdu , P. R. China.
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.affiliation: c Institute of Blood Transfusion, Chinese Academy of Medical Sciences , Chengdu , P. R. China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Zhou
authors.affiliation: c Institute of Blood Transfusion, Chinese Academy of Medical Sciences , Chengdu , P. R. China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Liu
authors.affiliation: c Institute of Blood Transfusion, Chinese Academy of Medical Sciences , Chengdu , P. R. China.
authors.forename: Chengmin
authors.initials: C
authors.lastname: Yang
authors.affiliation: a Department of Burn and Plastic Surgery , West China Hospital, Sichuan University , Chengdu , P. R. China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Xu
authors.affiliation: d Department of Anesthesiology , Chengdu Military General Hospital , Chengdu , P. R. China.
authors.forename: Gu
authors.initials: G
authors.lastname: Gong
authors.affiliation: d Department of Anesthesiology , Chengdu Military General Hospital , Chengdu , P. R. China.
authors.forename: Yinghai
authors.initials: Y
authors.lastname: Liu
authors.affiliation: d Department of Anesthesiology , Chengdu Military General Hospital , Chengdu , P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: b Laboratory of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University , Chengdu , Sichuan , P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
mesh_terms: D000818:Animals; D062186:Arterial Pressure / Q000187:drug effects / Q000502:physiology; D001692:Biological Transport; D002339:Carotid Arteries / Q000187:drug effects / Q000502:physiology; D003331:Coronary Vessels / Q000187:drug effects / Q000502:physiology; D003432:Cross-Linking Reagents / Q000737:chemistry; D004285:Dogs; D004337:Drug Carriers / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005263:Femoral Artery / Q000187:drug effects / Q000502:physiology; D005976:Glutaral / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology*; D058105:Polymerization; D061251:Primary Cell Culture; D014661:Vasoconstriction / Q000187:drug effects* / Q000502:physiology
chemical_list: D003432:Cross-Linking Reagents; D004337:Drug Carriers; D006454:Hemoglobins; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D010100:Oxygen; D005976:Glutaral
keywords: BKCa channels; hemoglobin-based oxygen carrier; patch clamp; vascular smooth muscle cells; vasoactivity