title: IL-2 inhibited the generation of CD4+ memory T cells.
abstract: The survival of T cells at different stages of development is dependent on extrinsic signals. IL-7 is necessary for the development of memory T cells. IL-7 could induce and maintain the differentiation, survival, and proliferation of CD4(+) memory T cells, and the roles of IL-2 and IL-15 in the generation of CD4(+) memory T cells were still unclear. A CD4(+) memory T cells in vitro generated system by adding IL-7. The phenotype of CD4(+) memory T cells was identified by FACS. The cells proliferation was analyzed by CFSE staining. The involved signal pathways were analyzed by Western blot. We found that IL-2, not IL-15, could inhibit CD4(+) memory T cells generation. Western blot showed that IL-7 up-regulated the P-STAT5A expression and down-regulated Bax expression, IL-2 reduced the effect of IL-7. Besides, IL-2-combined IL-7 up-regulated the P-AKT and Foxo3a expression a little. In conclusion, our data revealed the inhibitory role of IL-2 in CD4(+) memory T cells generation and indicated that PI3K/AKT signal pathway was involved.
authors.affiliation: Department of Immunology, National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, 800 Xiangyin Road, Shanghai, 200433, People's Republic of China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Lu
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Nie
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
authors.forename: Weimin
authors.initials: W
authors.lastname: Sun
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D015536:Down-Regulation / Q000187:drug effects; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000378:metabolism; D019409:Interleukin-15 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology*; D015851:Interleukin-7 / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000494:pharmacology; D010641:Phenotype; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D050799:STAT5 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; C474033:FoxO3 protein, mouse; D019409:Interleukin-15; D007376:Interleukin-2; D015851:Interleukin-7; D050799:STAT5 Transcription Factor; C494093:Stat5a protein, mouse; D051028:bcl-2-Associated X Protein; D010047:Ovalbumin; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt