title: Glutamate release from satellite glial cells of the murine trigeminal ganglion.
abstract: It has been proposed that glutamate serves as a mediator between neurons and satellite glial cells (SGCs) in sensory ganglia and that SGCs release glutamate. Using a novel method, we studied glutamate release from SGCs from murine trigeminal ganglia. Sensory neurons with adhering SGCs were enzymatically isolated from wild type and transgenic mice in which vesicular exocytosis was suppressed in glial cells. Extracellular glutamate was detected by microfluorimetry. After loading the cells with a photolabile Ca(2+) chelator, the intracellular Ca(2+) concentration was raised in SGCs by a UV pulse, which resulted in glutamate release. The amount of released glutamate was decreased in cells with suppressed exocytosis and after pharmacological block of hemichannels. The data demonstrate that SGCs of the trigeminal ganglion release glutamate in a Ca(2+)-dependent manner.
authors.affiliation: Paul-Flechsig-Institut für Hirnforschung, Universität Leipzig, Jahnallee 59, 04109 Leipzig, Germany.
authors.forename: Lysann
authors.initials: L
authors.lastname: Wagner
authors.affiliation: Laboratory of Experimental Surgery, Hadassah Hebrew University Medical Center, Mount Scopus, Jerusalem 91240, Israel.
authors.forename: Rebekah A
authors.initials: RA
authors.lastname: Warwick
authors.affiliation: Paul-Flechsig-Institut für Hirnforschung, Universität Leipzig, Jahnallee 59, 04109 Leipzig, Germany. Electronic address: thomas.pannicke@medizin.uni-leipzig.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Pannicke
authors.affiliation: Paul-Flechsig-Institut für Hirnforschung, Universität Leipzig, Jahnallee 59, 04109 Leipzig, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Reichenbach
authors.affiliation: Institut für Humangenetik, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Grosche
authors.affiliation: Laboratory of Experimental Surgery, Hadassah Hebrew University Medical Center, Mount Scopus, Jerusalem 91240, Israel.
authors.forename: Menachem
authors.initials: M
authors.lastname: Hanani
mesh_terms: D000818:Animals; D020013:Calcium Signaling; D005260:Female; D005971:Glutamates / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D027161:Satellite Cells, Perineuronal / Q000378:metabolism*; D055571:Secretory Pathway; D011984:Sensory Receptor Cells / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000378:metabolism*
chemical_list: D005971:Glutamates
keywords: Exocytosis; Gliotransmitter; Glutamate; Satellite glial cell; Trigeminal ganglion