title: Cytotoxic neviotane triterpene-type from the red sea sponge Siphonochalina siphonella.
abstract: BACKGROUND
  Siphonochalina siphonella is a marine sponge collected from saudi Red Sea water and scare study from this region.
  
  
  OBJECTIVE
  To isolate the anticancer triterpenes with potent cytotoxicity from marine sponge, Siphonochalina siphonella and state the mode of action in cancer cell lines.
  
  
  METHODS
  The sponge material was collected, extracted with organic solvent, and fractionated on different adsorbents. The structure of the pure metabolites were elucidated employing different spectroscopic techniques including 1D ((1)H and (13)C) and 2D (COSY, HMQC and HMBC) NMR, and MS spectroscopy.
  
  
  RESULTS
  A new Neviotine-C (1) was obtained, along with four known metabolites (2-5). All compounds, except 5, were tested towards MCF-7, PC-3 and A549 and showed effects with IC50 in range 7.9-87 μM, whilst, 3 showed potent anti-proliferative activity against PC-3 and A549 with IC50 = 7.9 ± 0.120 and 8.9 ± 0.010 μM, respectively.
  
  
  CONCLUSIONS
  Compounds (1-4) showed significant cytotoxic activities, while 3 showed potent effect. The antiproliferative of 3 was attributed to significant S-phase cell cycle arrest.
authors.affiliation: Department of Chemistry, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Rihab F
authors.initials: RF
authors.lastname: Angawi
authors.affiliation: Department of Chemistry, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Esraa
authors.initials: E
authors.lastname: Saqer
authors.affiliation: Department of Natural Products and Alternative Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia ; Department of Pharmacognosy, Minia University, Minia 61519, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abdel-Lateff
authors.affiliation: Department of Pharmacognosy, Mansoura University, Mansoura 35516, Egypt.
authors.forename: Farid A
authors.initials: FA
authors.lastname: Badria
authors.affiliation: Department of Chemistry, Dammietta University, New Dammietta, Egypt.
authors.forename: Seif-Eldin N
authors.initials: SE
authors.lastname: Ayyad
keywords: Marine; Siphonochalina; sponge; triterpenes and anticancer