title: A light-sheet microscope compatible with mobile devices for label-free intracellular imaging and biosensing.
abstract: The inner structure, especially the nuclear structure, of cells carries valuable information about disease and health conditions of a person. Here we demonstrate a label-free technique to enable direct observations and measurements of the size, shape and morphology of the cell nucleus. With a microfabricated lens and a commercial CMOS imager, we form a scanning light-sheet microscope to produce a dark-field optical scattering image of the cell nucleus that overlays with the bright-field image produced in a separate regime of the same CMOS sensor. We have used the device to detect nuclear features that characterize the life cycle of cells and have used the nucleus volume as a new parameter for cell classification. The device can be developed into a portable, low-cost, point-of-care device leveraging the capabilities of the CMOS imagers to be pervasive in mobile electronics.
authors.affiliation: Materials Science Program, University of California at San Diego, La Jolla, California 92093-0418, USA. tfwu@jacobs.ucsd.edu.
authors.forename: Tsung-Feng
authors.initials: TF
authors.lastname: Wu
authors.forename: Tony Minghung
authors.initials: TM
authors.lastname: Yen
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Han
authors.forename: Yu-Jui
authors.initials: YJ
authors.lastname: Chiu
authors.forename: Eason Y-S
authors.initials: EY
authors.lastname: Lin
authors.forename: Yu-Hwa
authors.initials: YH
authors.lastname: Lo
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D007962:Leukocytes; D008838:Microcomputers; D008853:Microscopy / Q000379:methods*