title: [Pathological changes in hepatocytes of mice with obesity-induced type 2 diabetes by monosodium glutamate].
abstract: Type 2 diabetes caused by chronic obesity is a major lifestyle-related disease. The present study aimed to determine the pathological changes in hepatocytes in chronic obesity. To develop our type 2 diabetes mouse model, we induced chronic obesity to mice by monosodium glutamate. By overeating, the mice significantly increased their body weight compared with age-matched healthy animals. To analyze the pathological changes in hepatocytes of chronic obesity before preclinical stage of type 2 diabetes, the mice were analyzed by hematoxylin-eosin staining of tissue sections at 15 w of age. In these mice, we observed eosin-negative accumulations of hepatocytes around central veins in the hepatic lobule. By Oil-Red O staining, the eosin-negative granules were identified in the lipid droplets. We then ascertained whether these lipid droplets of hepatocytes in the obese mice could be modified by diet. After 24 h of diet restriction, the lipid droplets of hepatocytes in the obese mice were swollen. Furthermore, after 48 h of the diet restriction, the lipid droplets continued swelling and the autophagy-like structures that were found in the healthy mice under the same condition in the obese mice were not observed. These results suggest that the obese mice might have delayed energy metabolism, which might have influenced the mechanisms of hepatocytes. These findings provide new insight into the functional changes in chronic obesity-induced type 2 diabetes and it is possible that the pathological feature make a contribution to promise the target of pharmacological therapy.
authors.affiliation: Faculty of Pharmaceutical Sciences, Meiji Pharmaceutical University.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakadate
authors.forename: Kento
authors.initials: K
authors.lastname: Motojima
authors.forename: Sumito
authors.initials: S
authors.lastname: Kamata
authors.forename: Testuro
authors.initials: T
authors.lastname: Yoshida
authors.forename: Masaaki
authors.initials: M
authors.lastname: Hikita
authors.forename: Hisanori
authors.initials: H
authors.lastname: Wakamatsu
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000139:chemically induced / Q000150:complications / Q000473:pathology*; D005260:Female; D005421:Flavoring Agents / Q000009:adverse effects; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology*; D002479:Inclusion Bodies / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000139:chemically induced / Q000150:complications*; D012970:Sodium Glutamate / Q000009:adverse effects
chemical_list: D005421:Flavoring Agents; D012970:Sodium Glutamate