title: Liposome-based liquid handling platform featuring addition, mixing, and aliquoting of femtoliter volumes.
abstract: This paper describes the utilization of giant unilamellar vesicles (GUVs) as a platform for handling chemical and biochemical reagents. GUVs with diameters of 5 to 10 µm and containing chemical/biochemical reagents together with inert polymers were fused with electric pulses (electrofusion). After reagent mixing, the fused GUVs spontaneously deformed to a budding shape, separating the mixed solution into sub-volumes. We utilized a microfluidic channel and optical tweezers to select GUVs of interest, bring them into contact, and fuse them together to mix and aliquot the reaction product. We also show that, by lowering the ambient temperature close to the phase transition temperature Tm of the lipid used, daughter GUVs completely detached (fission). This process performs all the liquid-handing features used in bench-top biochemistry using the GUV, which could be advantageous for the membrane-related biochemical assays.
authors.affiliation: Graduate School of Information Science and Technology, Osaka University, Osaka, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shiomi
authors.affiliation: School of Chemistry, University of Glasgow, Glasgow, United Kingdom; ERATO, JST, Tokyo, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Tsuda
authors.affiliation: ERATO, JST, Tokyo, Japan; Faculty of Science and Engineering, Chuo University, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Graduate School of Information Science and Technology, Osaka University, Osaka, Japan; ERATO, JST, Tokyo, Japan; Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yomo
mesh_terms: D008055:Lipids / Q000737:chemistry*; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010316:Particle Size; D044367:Phase Transition; D013696:Temperature; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D008055:Lipids; D008567:Membranes, Artificial; D053835:Unilamellar Liposomes