title: Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene.
abstract: Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.
authors.affiliation: Institute of Nano Science and Technology , Sector 64, Mohali 160064, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Sabherwal
authors.forename: Munish
authors.initials: M
authors.lastname: Shorie
authors.forename: Preeti
authors.initials: P
authors.lastname: Pathania
authors.forename: Shilpa
authors.initials: S
authors.lastname: Chaudhary
authors.forename: K K
authors.initials: KK
authors.lastname: Bhasin
authors.forename: Vijayender
authors.initials: V
authors.lastname: Bhalla
authors.forename: C Raman
authors.initials: CR
authors.lastname: Suri
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D031541:Fluorescence Resonance Energy Transfer; D008969:Molecular Sequence Data; D014303:Trinitrotoluene / Q000032:analysis*
chemical_list: D000906:Antibodies; D052157:Aptamers, Nucleotide; D014303:Trinitrotoluene