title: Assessment methods for angiogenesis and current approaches for its quantification.
abstract: Angiogenesis is a physiological process which describes the development of new blood vessels from the existing vessels. It is a common and the most important process in the formation and development of blood vessels, so it is supportive in the healing of wounds and granulation of tissues. The different assays for the evaluation of angiogenesis have been described with distinct advantages and some limitations. In order to develop angiogenic and antiangiogenic techniques, continuous efforts have been resulted to give animal models for more quantitative analysis of angiogenesis. Most of the studies on angiogenic inducers and inhibitors rely on various models, both in vitro, in vivo and in ova, as indicators of efficacy. The angiogenesis assays are very much helpful to test efficacy of both pro- and anti- angiogenic agents. The development of non-invasive procedures for quantification of angiogenesis will facilitate this process significantly. The main objective of this review article is to focus on the novel and existing methods of angiogenesis and their quantification techniques. These findings will be helpful to establish the most convenient methods for the detection, quantification of angiogenesis and to develop a novel, well tolerated and cost effective anti-angiogenic treatment in the near future.
authors.affiliation: Departments of Pharmacology and Toxicology, College of Pharmacy, Umm Al Qura University, Makkah, The Kingdom of Saudi Arabia.
authors.forename: Waleed Hassan
authors.initials: WH
authors.lastname: AlMalki
authors.affiliation: Departments of Pharmacology and Toxicology, College of Pharmacy, Umm Al Qura University, Makkah, The Kingdom of Saudi Arabia.
authors.forename: Imran
authors.initials: I
authors.lastname: Shahid
authors.affiliation: Departments of Pharmacology and Toxicology, College of Pharmacy, Umm Al Qura University, Makkah, The Kingdom of Saudi Arabia.
authors.forename: Abeer Yousaf
authors.initials: AY
authors.lastname: Mehdi
authors.affiliation: Fatima Memorial Hospital and Medical College, Shadman, Lahore, Pakistan.
authors.forename: Muhammad Hassan
authors.initials: MH
authors.lastname: Hafeez
mesh_terms: D000818:Animals; D001681:Biological Assay; D018929:Cell Culture Techniques; D006801:Humans; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D018919:Neovascularization, Physiologic
keywords: Angiogenesis; angiogenesis assays; quantification techniques