title: Alterations in the cholinergic system of brain stem neurons in a mouse model of Rett syndrome.
abstract: Rett syndrome is an autism-spectrum disorder resulting from mutations to the X-linked gene, methyl-CpG binding protein 2 (MeCP2), which causes abnormalities in many systems. It is possible that the body may develop certain compensatory mechanisms to alleviate the abnormalities. The norepinephrine system originating mainly in the locus coeruleus (LC) is defective in Rett syndrome and Mecp2-null mice. LC neurons are subject to modulation by GABA, glutamate, and acetylcholine (ACh), providing an ideal system to test the compensatory hypothesis. Here we show evidence for potential compensatory modulation of LC neurons by post- and presynaptic ACh inputs. We found that the postsynaptic currents of nicotinic ACh receptors (nAChR) were smaller in amplitude and longer in decay time in the Mecp2-null mice than in the wild type. Single-cell PCR analysis showed a decrease in the expression of α3-, α4-, α7-, and β3-subunits and an increase in the α5- and α6-subunits in the mutant mice. The α5-subunit was present in many of the LC neurons with slow-decay nAChR currents. The nicotinic modulation of spontaneous GABAA-ergic inhibitory postsynaptic currents in LC neurons was enhanced in Mecp2-null mice. In contrast, the nAChR manipulation of glutamatergic input to LC neurons was unaffected in both groups of mice. Our current-clamp studies showed that the modulation of LC neurons by ACh input was reduced moderately in Mecp2-null mice, despite the major decrease in nAChR currents, suggesting possible compensatory processes may take place, thus reducing the defects to a lesser extent in LC neurons.
authors.affiliation: Department of Biology, Georgia State University, Atlanta, Georgia.
authors.forename: Max F
authors.initials: MF
authors.lastname: Oginsky
authors.affiliation: Department of Biology, Georgia State University, Atlanta, Georgia.
authors.forename: Ningren
authors.initials: N
authors.lastname: Cui
authors.affiliation: Department of Biology, Georgia State University, Atlanta, Georgia.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Department of Biology, Georgia State University, Atlanta, Georgia.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Johnson
authors.affiliation: Department of Biology, Georgia State University, Atlanta, Georgia cjiang@gsu.edu.
authors.forename: Chun
authors.initials: C
authors.lastname: Jiang
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000222:Adaptation, Physiological; D000818:Animals; D059329:Cholinergic Neurons / Q000378:metabolism*; D004195:Disease Models, Animal; D019706:Excitatory Postsynaptic Potentials; D018698:Glutamic Acid / Q000378:metabolism; D053444:Inhibitory Postsynaptic Potentials; D007700:Kinetics; D008125:Locus Coeruleus / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D051783:Methyl-CpG-Binding Protein 2 / Q000172:deficiency / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018408:Patch-Clamp Techniques; D011963:Receptors, GABA-A / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism; D017661:Receptors, Presynaptic / Q000235:genetics / Q000378:metabolism; D015518:Rett Syndrome / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: C497393:Mecp2 protein, mouse; D051783:Methyl-CpG-Binding Protein 2; D011963:Receptors, GABA-A; D011978:Receptors, Nicotinic; D017661:Receptors, Presynaptic; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D000109:Acetylcholine
keywords: Mecp2; Rett syndrome; acetylcholine; compensatory mechanisms; locus coeruleus; nicotinic acetylcholine receptor