title: Genomic insights into the fungal lignocellulolytic system of Myceliophthora thermophila.
abstract: The microbial conversion of solid cellulosic biomass to liquid biofuels may provide a renewable energy source for transportation fuels. Cellulolytic fungi represent a promising group of organisms, as they have evolved complex systems for adaptation to their natural habitat. The filamentous fungus Myceliophthora thermophila constitutes an exceptionally powerful cellulolytic microorganism that synthesizes a complete set of enzymes necessary for the breakdown of plant cell wall. The genome of this fungus has been recently sequenced and annotated, allowing systematic examination and identification of enzymes required for the degradation of lignocellulosic biomass. The genomic analysis revealed the existence of an expanded enzymatic repertoire including numerous cellulases, hemicellulases, and enzymes with auxiliary activities, covering the most of the recognized CAZy families. Most of them were predicted to possess a secretion signal and undergo through post-translational glycosylation modifications. These data offer a better understanding of activities embedded in fungal lignocellulose decomposition mechanisms and suggest that M. thermophila could be made usable as an industrial production host for cellulolytic and hemicellulolytic enzymes.
authors.affiliation: Biotechnology Laboratory, Department of Synthesis and Development of Industrial Processes, School of Chemical Engineering, National Technical University of Athens Athens, Greece ; Biochemical Process Engineering, Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology Luleå, Sweden.
authors.forename: Anthi
authors.initials: A
authors.lastname: Karnaouri
authors.affiliation: Biotechnology Laboratory, Department of Synthesis and Development of Industrial Processes, School of Chemical Engineering, National Technical University of Athens Athens, Greece.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Topakas
authors.affiliation: Biochemical Process Engineering, Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology Luleå, Sweden.
authors.forename: Io
authors.initials: I
authors.lastname: Antonopoulou
authors.affiliation: Biochemical Process Engineering, Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology Luleå, Sweden.
authors.forename: Paul
authors.initials: P
authors.lastname: Christakopoulos
keywords: CAZy; Myceliophthora thermophila; biofuels; lignocellulolytic enzymes; plant biomass