title: Bone marrow mesenchymal stem cells ameliorate colitis-associated tumorigenesis in mice.
abstract: OBJECTIVE
  Bone marrow-derived mesenchymal stem cell (MSC) is widely studied in inflammatory bowel disease (IBD) in basic and clinical research. However, patients with IBD have higher risk of developing colorectal cancer and MSC has dual effect on tumorigenesis. This study aims to evaluate the role of MSC on tumorigenesis of IBD.
  
  
  METHODS
  MSCs were isolated from the bone marrow of allogenic mice and identified by flow cytometry. Mice in the model of colitis-associated tumorigenesis induced by azoxymethane and dextran sulfate sodium were injected with MSCs. Colon length, spleen size and tumors formation were assessed macroscopically. Pro-inflammatory cytokines and STAT3 phosphorylation in colon tissues were analyzed.
  
  
  RESULTS
  MSCs ameliorated the severity of colitis associated tumorigenesis compared with PBS control, with attenuated weight loss, longer colons and smaller spleens. Tumor number and tumor load were significantly less in the MSC group while tumor size remained comparable. Histological assessment indicated MSCs could reduce histological damage of the colon tissue. Decreased expression of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6), and down-regulation of STAT3 phosphorylation in colon tissue were found after MSC treatment.
  
  
  CONCLUSIONS
  MSCs might ameliorate the tumorigenesis of inflammatory bowel disease by suppression of expression of pro-inflammatory cytokines and STAT3 activation.
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou City, Guangdong Province, PR China.
authors.forename: Zexian
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou City, Guangdong Province, PR China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou City, Guangdong Province, PR China.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou City, Guangdong Province, PR China.
authors.forename: Xiuting
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou City, Guangdong Province, PR China.
authors.forename: Xutao
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou City, Guangdong Province, PR China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou City, Guangdong Province, PR China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou City, Guangdong Province, PR China. Electronic address: sumslp@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Lan
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D003092:Colitis / Q000150:complications*; D015179:Colorectal Neoplasms / Q000209:etiology / Q000601:surgery*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D018836:Inflammation Mediators / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation; D060888:Real-Time Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000378:metabolism; D033581:Stem Cell Transplantation
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse
keywords: Colitis; Inflammatory bowel disease; Mesenchymal stem cells; Mice; Tumorigenesis