title: [Expression and clinical significance of MMP-2 and MMP-9 in B acute lymphoblastic leukemia].
abstract: This study was purposed to investigate the expression and clinical significance of MMP-2 and MMP-9 in patients with B-acute lymphoblastic leukemia (B-ALL). The expression of MMP-2 and MMP-9 in bone marrow mononuclear cells of B-ALL patients and normal controls was detected by RT-PCR. The gelatinolytic activity was detected by zymography. The results showed that the expression of MMP-2 in de novo and relapsed B-ALL patients was markedly higher than that in normal controls (P < 0.05). The expression of MMP-9 in de novo and relapsed B-ALL patients was markedly lower than that in normal controls (P < 0.05). The expression of MMP-2 and MMP-9 in patients with extramedullary infiltration was significantly higher than that in patients without extramedullary infiltration. The incidence of extramedullary infiltration in patients with MMP-2/MMP-9 (+) was markedly higher than that in patients with MMP-2/MMP-9 (-). The expression of MMP-9 was markedly higher in high-risk patients than that in standard-risk patients (P < 0.05), but the expression of MMP-2 had no significant difference between the high-risk and standard-risk patients (P > 0.05). It is concluded that MMP-2 and MMP-9 may be secreted by B lymphoblasts and may involve in the extramedullary infiltration. MMP-9 may correlate with poor prognosis.
authors.affiliation: Hebei Province Key Laboratory of Blood Diseases, Department of Hematology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Pan
authors.affiliation: Hebei Province Key Laboratory of Blood Diseases, Department of Hematology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Hebei Province Key Laboratory of Blood Diseases, Department of Hematology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Shu-Peng
authors.initials: SP
authors.lastname: Wen
authors.affiliation: Hebei Province Key Laboratory of Blood Diseases, Department of Hematology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Liu
authors.affiliation: Hebei Province Key Laboratory of Blood Diseases, Department of Hematology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. E-mail: luojm315@163.com.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Luo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9