title: Proteome analysis of the HIV-1 Gag interactome.
abstract: Human immunodeficiency virus Gag drives assembly of virions in infected cells and interacts with host factors which facilitate or restrict viral replication. Although several Gag-binding proteins have been characterized, understanding of virus-host interactions remains incomplete. In a series of six affinity purification screens, we have identified protein candidates for interaction with HIV-1 Gag. Proteins previously found in virions or identified in siRNA screens for host factors influencing HIV-1 replication were recovered. Helicases, translation factors, cytoskeletal and motor proteins, factors involved in RNA degradation and RNA interference were enriched in the interaction data. Cellular networks of cytoskeleton, SR proteins and tRNA synthetases were identified. Most prominently, components of cytoplasmic RNA transport granules were co-purified with Gag. This study provides a survey of known Gag-host interactions and identifies novel Gag binding candidates. These factors are associated with distinct molecular functions and cellular pathways relevant in host-pathogen interactions.
authors.affiliation: Department of Infectious Diseases, Virology, Universitätsklinikum Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany. Electronic address: christine.engeland@urz.uni-heidelberg.de.
authors.forename: Christine E
authors.initials: CE
authors.lastname: Engeland
authors.affiliation: Bioquant, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany. Electronic address: nigel.brown@bioquant.uni-heidelberg.de.
authors.forename: Nigel P
authors.initials: NP
authors.lastname: Brown
authors.affiliation: Department of Infectious Diseases, Virology, Universitätsklinikum Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany; Bioquant, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany. Electronic address: kathleen.boerner@med.uni-heidelberg.de.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Börner
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Straße 10, D-13125 Berlin, Germany. Electronic address: schuemann@fmp-berlin.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Schümann
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Straße 10, D-13125 Berlin, Germany. Electronic address: ekrause@fmp-berlin.de.
authors.forename: Eberhard
authors.initials: E
authors.lastname: Krause
authors.affiliation: Institute for Medical Informatics and Biometry (IMB), Medical Faculty Carl Gustav Carus, Dresden University of Technology, Fetscherstraße 74, D-01307 Dresden, Germany. Electronic address: lars.kaderali@tu-dresden.de.
authors.forename: Lars
authors.initials: L
authors.lastname: Kaderali
authors.affiliation: Molecular Oncology, Medical School, University of Leipzig, Semmelweisstraße 14, D-04103 Leipzig, Germany. Electronic address: Gerd.Mueller@medizin.uni-leipzig.de.
authors.forename: Gerd A
authors.initials: GA
authors.lastname: Müller
authors.affiliation: Department of Infectious Diseases, Virology, Universitätsklinikum Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany. Electronic address: Hans-Georg.Kraeusslich@med.uni-heidelberg.de.
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Kräusslich
mesh_terms: D015658:HIV Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D011485:Protein Binding; D060066:Protein Interaction Maps; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism*
chemical_list: D020543:Proteome; D054301:gag Gene Products, Human Immunodeficiency Virus
keywords: HIV Gag; Virus–host interactions