title: Generation and identification of tumor-evoked regulatory B cells.
abstract: The involvement of Bregs in cancer remains poorly understood despite their well-documented regulation of responses to the self and protection from harmful autoimmunity. We recently discovered a unique regulatory B cell subset evoked by breast cancer to mediate protection of metastasizing cancer cells. These results together with the wealth of findings of the last 40 years on B cells in tumorigenesis suggest the existence of additional cancer Bregs modulating anticancer responses. To facilitate the search for them, here we provide our detailed protocol for the characterization and generation of tumor-evoked regulatory B cells. Wherever applicable, we also discuss nuances and uniqueness of a Breg study in cancer to warn potential pitfalls.
authors.affiliation: Immunoregulation Section, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD, 21224, USA, biragyna@mail.nih.gov.
authors.forename: Arya
authors.initials: A
authors.lastname: Biragyn
authors.forename: Catalina
authors.initials: C
authors.lastname: Lee-Chang
authors.forename: Monica
authors.initials: M
authors.lastname: Bodogai
mesh_terms: D000818:Animals; D060151:B-Lymphocytes, Regulatory / Q000276:immunology* / Q000473:pathology*; D063646:Carcinogenesis / Q000276:immunology / Q000473:pathology; D054414:Chemokine CCL17 / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D005098:Exotoxins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D051379:Mice; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D054414:Chemokine CCL17; D005098:Exotoxins; D011993:Recombinant Fusion Proteins; C000592234:TARC-PE38 fusion protein