title: Experimental studies on the inhibition of adenovirus-ING4-OSM therapy on nasopharyngeal carcinoma proliferation in vitro and in vivo.
abstract: In the present study, the effects of the co-transfer of the tumor growth inhibitor 4 gene (ING4) together with the Oncostatin M (OSM) were investigated on tumor regression and subsequent tumor recurrence. We constructed a recombinant adenovirus carrying ING4 and OSM, which could induce high-level expression of these three genes in NPC CNE-1 cells. Ad-ING4, Ad-OSM and Ad-ING4-OSM infection all inhibited the growth of CNE-1 cells in vitro, while the Ad-ING4-OSM exerted the strongest inhibitory effect. In CNE-1 xenograft tumor models mice, an intratumoral injection of Ad-ING4, Ad-OSM and Ad-ING4-OSM resulted in a reduced tumor burden, compared to normal saline controls. Therefore, we suggested that the introduction of adenovirus-mediated ING4 and OSM genes could synergistically decrease the recurrence or metastases and develop a control of NPC tumors, which advocate a promising therapeutic future in NPC treatment.
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, Chinese PLA General Hospital, Beijing, 100037, China, drzelihan@126.com.
authors.forename: Zeli
authors.initials: Z
authors.lastname: Han
authors.forename: Chengyong
authors.initials: C
authors.lastname: Zhou
authors.forename: Baochun
authors.initials: B
authors.lastname: Sun
authors.forename: Qinghong
authors.initials: Q
authors.lastname: Yan
authors.forename: Jinghong
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002277:Carcinoma; D018797:Cell Cycle Proteins / Q000235:genetics / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000627:therapeutic use*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy*; D053683:Oncostatin M / Q000235:genetics / Q000627:therapeutic use*; D014162:Transfection / Q000379:methods; D016896:Treatment Outcome; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000627:therapeutic use*
chemical_list: D018797:Cell Cycle Proteins; D018398:Homeodomain Proteins; C483230:ING4 protein, human; D025521:Tumor Suppressor Proteins; D053683:Oncostatin M