title: Adequacy of a compartment model for CMRO₂ quantitation using ¹⁵O-labeled oxygen and PET: a clearance measurement of ¹⁵O-radioactivity following intracarotid bolus injection of ¹⁵O-labeled oxyhemoglobin on Macaca fascicularis.
abstract: We aimed at evaluating the adequacy of the commonly employed compartmental model for quantitation of cerebral metabolic rate of oxygen (CMRO2) using (15)O-labeled oxygen ((15)O2) and positron emission tomography (PET). Sequential PET imaging was carried out on monkeys following slow bolus injection of blood samples containing (15)O2-oxyhemoglobin ((15)O2-Hb), (15)O-labeled water (H2(15)O), and C(15)O-labeled hemoglobin (C(15)O-Hb) into the internal carotid artery (ICA). Clearance slopes were assessed in the middle cerebral artery territory of the injected hemisphere. The time-activity curves were bi-exponential for both (15)O2-Hb and H2(15)O. Single exponential fitting to the early (5 to 40 seconds) and late (80 to 240 seconds) periods after the peak was performed and the (15)O2-Hb and H2(15)O results were compared. It was found that a significant difference between the clearance rates of the (15)O2-Hb and H2(15)O injections is unlikely, which supports the mathematical model that is widely used to describe the kinetics of (15)O2-Hb and H2(15)O in cerebral tissues and is the basis of recent approaches to simultaneously assess CMRO2 and cerebral blood flow in a single PET session. However, it should be noted that more data are necessary to unequivocally confirm the result.
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Iida
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Iguchi
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Noboru
authors.initials: N
authors.lastname: Teramoto
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Koshino
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Zeniya
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Akihide
authors.initials: A
authors.lastname: Yamamoto
authors.affiliation: 1] Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan [2] Department of Medical Physics, Faculty of Medicine, Kagawa University, Kitagun, Kagawa, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kudomi
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Tetsuaki
authors.initials: T
authors.lastname: Moriguchi
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hori
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Enmi
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Kawashima
authors.affiliation: Institute of Neuroscience and Medicine 4, Medical Imaging Physics, Forschungszentrum Jülich GmbH, Jülich, Germany.
authors.forename: Nadim
authors.initials: N
authors.lastname: Joni Shah
authors.affiliation: Integrative Stroke Imaging Center, Department of Neurosurgery, National Cerebral and Cardiovascular Center, Suit City, Osaka, Japan.
authors.forename: Jyoji
authors.initials: J
authors.lastname: Nakagawara
mesh_terms: D000818:Animals; D002533:Cerebral Angiography / Q000379:methods*; D002560:Cerebrovascular Circulation; D007553:Isotope Labeling; D008253:Macaca mulatta; D008297:Male; D010103:Oxygen Isotopes / Q000737:chemistry / Q000494:pharmacology; D010108:Oxyhemoglobins / Q000737:chemistry / Q000494:pharmacology*; D049268:Positron-Emission Tomography / Q000379:methods*
chemical_list: D010103:Oxygen Isotopes; D010108:Oxyhemoglobins