title: Oxidative stress in mouse sperm impairs embryo development, fetal growth and alters adiposity and glucose regulation in female offspring.
abstract: Paternal health cues are able to program the health of the next generation however the mechanism for this transmission is unknown. Reactive oxygen species (ROS) are increased in many paternal pathologies, some of which program offspring health, and are known to induce DNA damage and alter the methylation pattern of chromatin. We therefore investigated whether a chemically induced increase of ROS in sperm impairs embryo, pregnancy and offspring health. Mouse sperm was exposed to 1500 µM of hydrogen peroxide (H2O2), which induced oxidative damage, however did not affect sperm motility or the ability to bind and fertilize an oocyte. Sperm treated with H2O2 delayed on-time development of subsequent embryos, decreased the ratio of inner cell mass cells (ICM) in the resulting blastocyst and reduced implantation rates. Crown-rump length at day 18 of gestation was also reduced in offspring produced by H2O2 treated sperm. Female offspring from H2O2 treated sperm were smaller, became glucose intolerant and accumulated increased levels of adipose tissue compared to control female offspring. Interestingly male offspring phenotype was less severe with increases in fat depots only seen at 4 weeks of age, which was restored to that of control offspring later in life, demonstrating sex-specific impacts on offspring. This study implicates elevated sperm ROS concentrations, which are common to many paternal health pathologies, as a mediator of programming offspring for metabolic syndrome and obesity.
authors.affiliation: Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health and Robinson Institute, University of Adelaide, South Australia, Australia; Repromed, Dulwich, South Australia, Australia.
authors.forename: Michelle
authors.initials: M
authors.lastname: Lane
authors.affiliation: Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health and Robinson Institute, University of Adelaide, South Australia, Australia.
authors.forename: Nicole O
authors.initials: NO
authors.lastname: McPherson
authors.affiliation: Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health and Robinson Institute, University of Adelaide, South Australia, Australia.
authors.forename: Tod
authors.initials: T
authors.lastname: Fullston
authors.affiliation: Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health and Robinson Institute, University of Adelaide, South Australia, Australia.
authors.forename: Marni
authors.initials: M
authors.lastname: Spillane
authors.affiliation: Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health and Robinson Institute, University of Adelaide, South Australia, Australia.
authors.forename: Lauren
authors.initials: L
authors.lastname: Sandeman
authors.affiliation: Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health and Robinson Institute, University of Adelaide, South Australia, Australia.
authors.forename: Wan Xian
authors.initials: WX
authors.lastname: Kang
authors.affiliation: Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health and Robinson Institute, University of Adelaide, South Australia, Australia; Repromed, Dulwich, South Australia, Australia.
authors.forename: Deirdre L
authors.initials: DL
authors.lastname: Zander-Fox
mesh_terms: D050154:Adiposity; D000818:Animals; D001755:Blastocyst / Q000166:cytology; D047108:Embryonic Development; D005260:Female; D005306:Fertilization; D047109:Fetal Development; D005947:Glucose / Q000378:metabolism*; D018149:Glucose Intolerance; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018384:Oxidative Stress; D011247:Pregnancy; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D006861:Hydrogen Peroxide; D005947:Glucose