title: Effects of Aroclor 1254 on in vivo oocyte maturation in the mouse.
abstract: Polychlorinated biphenyls (PCBs) are stable, lipophilic compounds that accumulate in the environment and in the food chain. Though some studies provided evidence that PCBs had adverse effects on reproductive function, most of these results were from in vitro models. Therefore we investigated the effect of Aroclor 1254 (a commercial PCBs mixture) treatments on in vivo maturation and developmental potential of mouse oocytes. In the present study, female ICR mice were treated with different doses (12.5, 25 and 50 mg/kg) of Aroclor 1254 (a commercial PCB mixture) once every 72 hours by intraperitoneal injection for 9 days. After three treatments of Aroclor 1254, the mice were superovulated to collect oocytes one day after the last exposure. The effects of Aroclor 1254 on oocyte maturation, fertilization, and preimplantation embryonic development were investigated. Immunofluorescence-stained oocytes were observed under a confocal microscope to assess the effects of Aroclor 1254 on spindle morphology. Parthenogenic activation and the incidence of cumulus apoptosis in cumulus-oocyte complexes were observed as well. Oocytes exposed to different doses of Aroclor 1254 in vivo were associated with a significant decrease in outgrowth potential, abnormal spindle configurations, and the inhibition of parthenogenetic activation of ovulated oocytes. Furthermore, the incidence of apoptosis in cumulus cells was increased after exposed to Aroclor 1254. These results may provide reference for the treatment of reproductive diseases such as infertility or miscarriage caused by environmental contaminants.
authors.affiliation: Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, China.
authors.forename: ShuZhen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Center for Reproductive Medicine, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: LiGang
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, China.
authors.forename: XiaoQian
authors.initials: X
authors.lastname: Meng
authors.affiliation: Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan, China.
authors.forename: XiaoYing
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Toxicology, Shandong Center for Disease Control and Prevention, Jinan, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Cheng
authors.affiliation: Department of Toxicology, Shandong Center for Disease Control and Prevention, Jinan, China.
authors.forename: TianLiang
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: YiLiang
authors.initials: Y
authors.lastname: Miao
mesh_terms: D000818:Animals; D020111:Chlorodiphenyl (54% Chlorine) / Q000494:pharmacology*; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005306:Fertilization / Q000187:drug effects; D008297:Male; D051379:Mice; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects*; D011247:Pregnancy
chemical_list: D020111:Chlorodiphenyl (54% Chlorine)