title: Development of a liquid chromatographic method for the quantification of paromomycin. Application to in vitro release and ex vivo permeation studies.
abstract: We have developed a reversed phase high performance liquid chromatography pulsed amperometric detection (RPHPLC-PAD) method for the determination of paromomycin. It is sensitive, repeatable, and selective without the pretreatment step. Trifluoroacetic acid-water was utilized as the eluent and detected by PAD under NaOH alkaline conditions. The paromomycin detection limit (S/N=3.3) was 2μgmL(-1) and the quantification limit (S/N=10) was 6μgmL(-1). Coefficients of linear regression were higher than 0.99 for concentrations between 6.25 and 200μgmL(-1). The intra and inter-day precision (RSD) was less than 6.5%. The average recoveries were 97.53-102.01%. The proposed HPLC-PAD method presented advantageous performance characteristics and it can be considered suitable for the evaluation of paromomycin loaded nanogel formulation in ex vivo permeation and in vitro release studies.
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Pharmacy, University of Barcelona, Joan XXIII Ave., 08028 Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Pujol-Brugués
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Barcelona, Joan XXIII Ave., 08028 Barcelona, Spain.
authors.forename: A C
authors.initials: AC
authors.lastname: Calpena-Campmany
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Pharmacy, University of Barcelona, Joan XXIII Ave., 08028 Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Riera-Lizandra
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Barcelona, Joan XXIII Ave., 08028 Barcelona, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Halbaut-Bellowa
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, Campus of Cartuja St., 18071 Granada, Spain. Electronic address: beatrizclares@ugr.es.
authors.forename: B
authors.initials: B
authors.lastname: Clares-Naveros
mesh_terms: D000563:Amebicides / Q000032:analysis* / Q000493:pharmacokinetics; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000493:pharmacokinetics; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D010303:Paromomycin / Q000032:analysis* / Q000493:pharmacokinetics; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption
chemical_list: D000563:Amebicides; D000900:Anti-Bacterial Agents; D010303:Paromomycin
keywords: Cutaneous leishmaniasis; Paromomycin; Pulsed amperometric detection; RPHPLC