title: Tracking the degradation of fresh orange juice and discrimination of orange varieties: an example of NMR in coordination with chemometrics analyses.
abstract: Brazil is currently the largest exporter of concentrated orange juice and, unlike the other exporter countries, the domestic consumption is mainly based on the fresh orange juice. The quality control by evaluating the major chemical constituents under the influence of the most important factors, such as temperature and storage time of the product, is very important in this context. Therefore, the objective of this study was to evaluate the influence of temperature and time on the degradation of fresh orange juice for 24h, by using (1)H NMR technique and chemometric tools for data mining. The storage conditions at 24h led to the production of the formic, fumaric and acetic acids; and an increase of succinic and lactic acids and ethanol, which were observed at low concentration at the initial time. Furthermore, analysis by PCA has successfully distinguished the juice of different species/varieties as well as the metabolites responsible for their separation.
authors.affiliation: Federal University of São Carlos, Department of Chemistry, Rodovia Washington Luís km 235, 13565-905 São Carlos, SP, Brazil. Electronic address: oliveira.cr_12@yahoo.com.br.
authors.forename: Clayton R
authors.initials: CR
authors.lastname: de Oliveira
authors.affiliation: Federal University of São Carlos, Department of Chemistry, Rodovia Washington Luís km 235, 13565-905 São Carlos, SP, Brazil.
authors.forename: Renato L
authors.initials: RL
authors.lastname: Carneiro
authors.affiliation: Federal University of São Carlos, Department of Chemistry, Rodovia Washington Luís km 235, 13565-905 São Carlos, SP, Brazil.
authors.forename: Antonio G
authors.initials: AG
authors.lastname: Ferreira
mesh_terms: D001628:Beverages / Q000032:analysis*; D001938:Brazil; D032084:Citrus sinensis / Q000737:chemistry; D000431:Ethanol / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D025341:Principal Component Analysis; D011786:Quality Control; D013696:Temperature
chemical_list: D000431:Ethanol
keywords: (1)H NMR fingerprint; Food degradation; Fresh orange juice; Multivariate data analysis