title: Expression and antigenic characterization of bubaline herpesvirus 1 (BuHV1) glycoprotein E and its potential application in the epidemiology and control of alphaherpesvirus infections in Mediterranean water buffalo.
abstract: Bubaline herpesvirus 1 (BuHV1) is a member of ruminant alphaherpesviruses antigenically related to bovine herpesvirus 1 (BoHV1). The impact of BuHV1 infection in infectious bovine rhinotracheitis control program is difficult to establish, due to the lack of specific diagnostic test. The ectodomain of glycoprotein E of BuHV1 was expressed as recombinant secreted protein and used in indirect ELISA as well as in a discriminatory test using the BoHV1 counterpart. A panel of monoclonal antibodies was produced against BuHV1; 6 out of 7 anti-gE monoclonal antibodies specifically recognized the BuHV1 gE. Results indicated BuHV1 gE as a sensitive marker of infection compared to seroneutralization (SN) test or blocking ELISA. When BoHV1 and BuHV1 gEs were immobilized in different wells of the same ELISA microplate, bovine and water buffalo sera were more reactive against the respective infecting virus. About one third of seropositive buffaloes with no history of contact with cattle and having higher SN titres, reacted in BoHV1 gE blocking ELISA, possibly because of steric hindrance. Since in two occasions BuHV1 was also isolated from water buffalo scoring gB+/gE+ BoHV1 blocking ELISA, we conclude that the combination of the two blocking ELISAs is not suitable to differentiate between BoHV1 and BuHV1.
authors.affiliation: Dipartimento di Scienze Veterinarie, Università degli Studi di Torino, Grugliasco, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Nogarol
authors.affiliation: Dipartimento di Scienze Veterinarie, Università degli Studi di Torino, Grugliasco, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Bertolotti
authors.affiliation: Istituto Zooprofilattico Sperimentale del Mezzogiorno, Centro di Referenza Nazionalesull'igiene e le tecnologie dell'allevamento e delle produzioni bufaline, Salerno, Italy.
authors.forename: E
authors.initials: E
authors.lastname: De Carlo
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, Torino, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Masoero
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, Torino, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Caruso
authors.affiliation: Dipartimento di Scienze Veterinarie, Università degli Studi di Torino, Grugliasco, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Profiti
authors.affiliation: Istituto Zooprofilattico Sperimentale del Mezzogiorno, Centro di Referenza Nazionalesull'igiene e le tecnologie dell'allevamento e delle produzioni bufaline, Salerno, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Martucciello
authors.affiliation: Istituto Zooprofilattico Sperimentale del Mezzogiorno, Centro di Referenza Nazionalesull'igiene e le tecnologie dell'allevamento e delle produzioni bufaline, Salerno, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Galiero
authors.affiliation: Istituto Zooprofilattico della Lombardia e dell'Emilia Romagna, Brescia, Italy; Istituto Zooprofilattico delle Venezie, Legnaro, Padova, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Cordioli
authors.affiliation: Istituto Zooprofilattico della Lombardia e dell'Emilia Romagna, Brescia, Italy; Istituto Zooprofilattico delle Venezie, Legnaro, Padova, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Lelli
authors.affiliation: Istituto Zooprofilattico della Lombardia e dell'Emilia Romagna, Brescia, Italy; Istituto Zooprofilattico delle Venezie, Legnaro, Padova, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Nardelli
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, Torino, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Ingravalle
authors.affiliation: Dipartimento di Scienze Veterinarie, Università degli Studi di Torino, Grugliasco, Italy. Electronic address: sergio.rosati@unito.it.
authors.forename: S
authors.initials: S
authors.lastname: Rosati
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics / Q000302:isolation & purification; D002020:Buffaloes; D003429:Cross Reactions; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000628:therapy / Q000662:veterinary*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D018140:Varicellovirus / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins
keywords: Bubaline herpesvirus 1; ELISA; Recombinant glycoprotein E