title: Formation of a flavin-linked peptide.
abstract: In a previous study, we showed that formylmethylflavin (FMF) can bind to cysteine. In this study, FMF was reacted with native peptides (CG and CKLVFF) containing an N-terminal cysteine. The formation of flavin-CG and flavin-CKLVFF was confirmed using HPLC and ESI-MS. Storage of flavin-CKLVFF in DMSO at -30 °C for 7 days resulted in no detectable deposition. In contrast, flavin-CKLVFF formed deposits when stored in water at -30 °C for 1 day, but no deposit was observed in the aqueous solution of flavin-CKLVFF after 7 days storage in the presence of 0.1% Triton X-100.
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan. s110702@stu.bunri-u.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Morikawa
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan. kkino@kph.bunri-u.ac.jp.
authors.forename: Katsuhito
authors.initials: K
authors.lastname: Kino
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan. s068064@stu.bunri-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Senda
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan. s120002@stu.bunri-u.ac.jp.
authors.forename: Masayo
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan. kobayashit@kph.bunri-u.ac.jp.
authors.forename: Takanobu
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan. miyazawah@kph.bunri-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyazawa
mesh_terms: D000595:Amino Acid Sequence; D004151:Dipeptides / Q000737:chemistry*; D005415:Flavins / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D011485:Protein Binding
chemical_list: C115829:10-formylmethylflavin; D004151:Dipeptides; D005415:Flavins