title: Cytotoxic activity of acyl phloroglucinols isolated from the leaves of Eucalyptus cinerea F. Muell. ex Benth. cultivated in Egypt.
abstract: Two acyl phloroglucinol compounds namely; Sideroxylonal B (1) and Macrocarpal A (2) were isolated from the Sideroxylonal-Rich Extract (SRE) of the juvenile leaves of Eucalyptus cinerea; F. Muell. ex Benth cultivated in Egypt. Identification of the isolated compounds was established on the basis of physico-chemical properties and spectral analysis (1D & 2D NMR). The two compounds were isolated for the first time from this species. The SRE alongside with the isolated compounds were tested against three human cancer cell lines; MCF7 (breast carcinoma cell line), HEP2 (laryngeal carcinoma), CaCo (colonic adenocarcinoma) and one type of normal human cell line;10 FS (fibroblast cells). The SRE, (1), and (2) showed cytotoxic activity with IC₅₀ 13.6 ± 0.62, 7.2 ± 0.5, 14.8 ± 0.55 μg mL-1 against HEP2 respectively, 11.6 ± 0.47, 4 ± 0.36, 11.4 ± 0.45 μg mL-1 against CaCo, respectively, and 8.6 ± 0.29, 4.4 ± 0.25, and 7.8 ± 0.3 μg mL-1 against MCF7, respectively. Meanwhile, the (SRE) together with (1) and (2) exhibited low cytotoxicity against normal cell line 10 FS, with IC₅₀ 55.4 ± 1.4, 43 ± 0.8 and 50.1 ± 1.12 μg mL-1, respectively. The antiprofilerative activity of the tested compounds was evaluated. The cell cycle profile of cells treated with Sideroxylonal-B and Macrocarpal-A indicates possible S-phase specific effects.
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy,Cairo University, Kasr el-Aini street, 11562, Cairo, Egypt.
authors.forename: Fathy M
authors.initials: FM
authors.lastname: Soliman
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy,Cairo University, Kasr el-Aini street, 11562, Cairo, Egypt.
authors.forename: Magda M
authors.initials: MM
authors.lastname: Fathy
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy,Cairo University, Kasr el-Aini street, 11562, Cairo, Egypt.
authors.forename: Maha M
authors.initials: MM
authors.lastname: Salama
authors.affiliation: 1] Pharmacology Department, Medical Division, National Research Center, Giza, Egypt [2] Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Al-Abd
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy,Cairo University, Kasr el-Aini street, 11562, Cairo, Egypt.
authors.forename: Fatema R
authors.initials: FR
authors.lastname: Saber
authors.affiliation: 1] Pharmacognosy Department, Faculty of Pharmacy,Cairo University, Kasr el-Aini street, 11562, Cairo, Egypt [2] Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
authors.forename: Ali M
authors.initials: AM
authors.lastname: El-Halawany
mesh_terms: D000215:Acylation; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004534:Egypt; D005052:Eucalyptus / Q000737:chemistry* / Q000145:classification; D006801:Humans; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology*; D010696:Phloroglucinol / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013045:Species Specificity; D016896:Treatment Outcome
chemical_list: D010936:Plant Extracts; D010696:Phloroglucinol