title: Microwave-assisted extraction of green coffee oil and quantification of diterpenes by HPLC.
abstract: The microwave-assisted extraction (MAE) of 13 different green coffee beans (Coffea arabica L.) was compared to Soxhlet extraction for oil obtention. The full factorial design applied to the microwave-assisted extraction (MAE), related to time and temperature parameters, allowed to develop a powerful fast and smooth methodology (10 min at 45°C) compared to a 4h Soxhlet extraction. The quantification of cafestol and kahweol diterpenes present in the coffee oil was monitored by HPLC/UV and showed satisfactory linearity (R(2)=0.9979), precision (CV 3.7%), recovery (<93%), limit of detection (0.0130 mg/mL), and limit of quantification (0.0406 mg/mL). The space-time yield calculated on the diterpenes content for sample AT1 (Arabica green coffee) showed a six times higher value compared to the traditional Soxhlet method.
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Química, Bloco A, CEP: 21941-909 Rio de Janeiro - RJ, Brazil. Electronic address: annatsukui@ufrj.br.
authors.forename: A
authors.initials: A
authors.lastname: Tsukui
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Química, Bloco A, CEP: 21941-909 Rio de Janeiro - RJ, Brazil. Electronic address: martinssantosjr@yahoo.com.br.
authors.forename: H M
authors.initials: HM
authors.lastname: Santos Júnior
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Química, Bloco A, CEP: 21941-909 Rio de Janeiro - RJ, Brazil. Electronic address: silvia.oigman@gmail.com.
authors.forename: S S
authors.initials: SS
authors.lastname: Oigman
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Química, Bloco A, CEP: 21941-909 Rio de Janeiro - RJ, Brazil. Electronic address: rodrigosouza@iq.ufrj.br.
authors.forename: R O M A
authors.initials: RO
authors.lastname: de Souza
authors.affiliation: Embrapa Agroindústria de Alimentos, Av. das Américas, 29501, CEP: 23020-470 Rio de Janeiro, Brazil. Electronic address: bizzo@ctaa.embrapa.br.
authors.forename: H R
authors.initials: HR
authors.lastname: Bizzo
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Química, Bloco A, CEP: 21941-909 Rio de Janeiro - RJ, Brazil. Electronic address: crezende@iq.ufrj.br.
authors.forename: C M
authors.initials: CM
authors.lastname: Rezende
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D040503:Coffea / Q000737:chemistry*; D004224:Diterpenes / Q000032:analysis* / Q000302:isolation & purification*; D008872:Microwaves; D010938:Plant Oils / Q000032:analysis* / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D004224:Diterpenes; D010938:Plant Oils
keywords: Cafestol; Coffee diterpenes; Coffee oil; Kahweol; Microwave-assisted extraction