title: Production of acylated homoserine lactone by a novel marine strain of Proteus vulgaris and inhibition of its swarming by phytochemicals.
abstract: A marine strain of Proteus vulgaris capable of activating multiple acylated homoserine lactone (AHL)-based reporter cultures was isolated. The cognate signal molecule was characterized as octanoyl homoserine lactone (OHL) and its production was observed to be growth dependent, with maximum production (5.675 µg l(-1)) at 24 h growth. The strain exhibited swarming, but its motility was not affected upon addition of pure OHL or culture supernatant. Phytochemicals such as quercitin and berberine chloride inhibited OHL production and reduced swarming. FliA, the predominantly upregulated protein during swarming, was considered as a possible target for these inhibitors, and docking of the two most active and two least active inhibitors to this protein suggested preferential binding of the former set of compounds. Apart from adding new evidence to AHL production in Proteus vulgaris, active inhibitors shortlisted from this study could help in identifying lead compounds to act against this opportunistic pathogen of the respiratory and gastrointestinal tract.
authors.affiliation: Bioengineering and Drug Design Lab, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology (IIT) Madras, Chennai 600036, Tamil Nadu, India.
authors.forename: Pramal
authors.initials: P
authors.lastname: Biswa
authors.affiliation: Bioengineering and Drug Design Lab, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology (IIT) Madras, Chennai 600036, Tamil Nadu, India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Doble
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000378:metabolism; D001599:Berberine / Q000494:pharmacology; D008124:Locomotion / Q000187:drug effects*; D064209:Phytochemicals / Q000494:pharmacology*; D011514:Proteus vulgaris / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D011794:Quercetin / Q000494:pharmacology
chemical_list: D054742:Acyl-Butyrolactones; D000900:Anti-Bacterial Agents; D064209:Phytochemicals; D001599:Berberine; D011794:Quercetin