title: Characterization of the interaction between lysyl-tRNA synthetase and laminin receptor by NMR.
abstract: Lysyl-tRNA synthetase (KRS) interacts with the laminin receptor (LR/RPSA) and enhances laminin-induced cell migration in cancer metastasis. In this nuclear magnetic resonance (NMR)-based study, we show that the anticodon-binding domain of KRS binds directly to the C-terminal region of 37LRP, and the previously found inhibitors BC-K-01 and BC-K-YH16899 interfere with KRS-37LRP binding. In addition, the anticodon-binding domain of KRS binds to laminin, observed by NMR and SPR. These results provide crucial insights into the structural characteristics of the KRS-LR interaction on the cell surface.
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 339-700, Republic of Korea.
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Cho
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 339-700, Republic of Korea.
authors.forename: Ameeq
authors.initials: A
authors.lastname: Ul Mushtaq
authors.affiliation: Medicinal Bioconvergence Research Center, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Medicinal Bioconvergence Research Center, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Dae Gyu
authors.initials: DG
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 339-700, Republic of Korea.
authors.forename: Min Sook
authors.initials: MS
authors.lastname: Seok
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Minseok
authors.initials: M
authors.lastname: Jang
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Byung-Woo
authors.initials: BW
authors.lastname: Han
authors.affiliation: Medicinal Bioconvergence Research Center, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 339-700, Republic of Korea. Electronic address: yhjeon@korea.ac.kr.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Jeon
mesh_terms: D000926:Anticodon / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D008250:Lysine-tRNA Ligase / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017474:Receptors, Laminin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000926:Anticodon; D010446:Peptide Fragments; D017474:Receptors, Laminin; D008250:Lysine-tRNA Ligase
keywords: Laminin; Laminin receptor; Lysyl-tRNA synthetase; Nuclear magnetic resonance