title: Inhibition of EZH2 expression is associated with the proliferation, apoptosis, and migration of SW620 colorectal cancer cells in vitro.
abstract: Epigenetic changes have been recently recognized as important in many human cancers. Enhancer of zeste homologue 2 (EZH2)gene has shown overexpression in various human cancers, consistent with a straightforward role of EZH2 as an oncogene, but its function in carcinogenesis is partly contradictory. The role of EZH2 in development of human colorectal cancer (CRC) has not yet been clarified. In present study, we observed up-regulation of EZH2 expression in tumor tissues from CRC patients [corrected]. The expression of EZH2 in CRC cell lines is consistent with the trend in cancer tissues using RT-PCR. We showed that TNM stage and lymph node metastasis in CRC patients are significantly correlated with EZH2 expression levels [corrected]. EZH2 level of transcription and protein was inhibited by small interfering RNA (siRNA). More importantly, EZH2-siRNA inhibited the proliferation and migration of SW620 cells while promoting their apoptosis, and inducing G0/G1 cell cycle arrest of CRC cells. Collectively, our results suggest that upregulated EZH2 expression may contribute to the progression of the patients with CRC. A comprehensive study of epigenetic mechanisms and the relevance of EZH2 in CRC is important for fully understanding this disease and as a basis for developing new treatment options in patients with CRC [corrected].
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China captain_hsb@163.com idechun1955@163.com.
authors.forename: Song-Bing
authors.initials: SB
authors.lastname: He
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Gastrointestinal Surgery, Changshu No. 2 Hospital, Suzhou 215500, China.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Tuo
authors.initials: T
authors.lastname: Han
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China.
authors.forename: Dai-Wei
authors.initials: DW
authors.lastname: Wan
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Gu
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Xue
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Zhang
authors.affiliation: Jiangsu Institute Hematology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Min
authors.initials: M
authors.lastname: Fei
authors.affiliation: Department of Clinical Laboratories, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
authors.forename: Shui-Qing
authors.initials: SQ
authors.lastname: Hu
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Yang
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China captain_hsb@163.com idechun1955@163.com.
authors.forename: Xin-Guo
authors.initials: XG
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: De-Chun
authors.initials: DC
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D014408:Biomarkers, Tumor / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D000071221:Enhancer of Zeste Homolog 2 Protein; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D063151:Polycomb Repressive Complex 2 / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000378:metabolism; D011379:Prognosis; D034741:RNA, Small Interfering / Q000494:pharmacology; D015854:Up-Regulation / Q000502:physiology
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2
keywords: EZH2; apoptosis; colorectal cancer; migration; proliferation; small interfering RNA