title: Construction and characteristics of an E-cadherin-related three-dimensional suspension growth model of ovarian cancer.
abstract: Ovarian cancer is the deadliest of all gynecologic malignancies. Metastatic ovarian cancer cells exist mainly in the form of multi-cellular spheroids (MCSs) in the ascites of patients with advanced ovarian cancer. We hypothesized that E-cadherin, as an important cell-adhesion molecule, might play an important role in the formation and survival of MCSs. Therefore, we established a three-dimensional suspension culture model of ovarian cancer cells that express high levels of E-cadherin to investigate their growth, proliferation, and resistance to chemotherapeutic drugs by CCK-8 assays. Compared to the cell suspension masses formed by cells with low or absent E-cadherin expression, the MCSs of high E-cadherin SKOV-3 cells had larger volumes, tighter cellular connections, and longer survival times. Although the suspension cell masses of all three cell lines were proliferatively stagnant, possibly due to cell cycle arrest at G1/S, cell mortality at 72 h after cisplatin treatment was significantly decreased in the high E-cadherin SKOV-3 cells compared to SKOV-3 cells without E-cadherin expression and to OVCAR-3 cells with low E-cadherin expression. We conclude, therefore, E-cadherin plays a vital role in MCS formation, maintenance, and drug resistance in ovarian cancer and could be a potential target for late-stage ovarian cancer treatment.
authors.affiliation: Department of Chemotherapy, Cancer Center, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Chemotherapy, Cancer Center, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Ya'nan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Cancer, Weifang Traditional Chinese Medical Hospital, Weifang, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Medicine, Medical School of Shandong University, Jinan, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Qiu
authors.affiliation: Department of Chemotherapy, Cancer Center, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Chemotherapy, Cancer Center, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Chemotherapy, Cancer Center, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Liu
mesh_terms: D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D018929:Cell Culture Techniques; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002945:Cisplatin / Q000494:pharmacology; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects / Q000502:physiology; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*; D018874:Spheroids, Cellular / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; D002945:Cisplatin