title: Protective effects of Baccharis dracunculifolia leaves extract against carbon tetrachloride- and acetaminophen-induced hepatotoxicity in experimental animals.
abstract: In this work we investigated the in vivo protective effects of Baccharis dracunculifolia leaves extract (BdE) against carbon tetrachloride (CCl4)- and acetaminophen (APAP)-induced hepatotoxicity. Total phenolic content, total flavonoid content, antioxidant DPPH radical scavenging activity, and HPLC analysis were performed. Our results showed that pretreatment with BdE significantly reduced the damage caused by CCl4 and APAP on the serum markers of hepatic injury, AST, ALT, and ALP. Results were confirmed by histopathological analysis. Phytochemical analysis, performed by HPLC, showed that BdE was rich in p-coumaric acid derivatives, caffeoylquinic acids and flavonoids. BdE also showed DPPH antioxidant activity (EC50 of 15.75±0.43 μg/mL), and high total phenolic (142.90±0.77 mg GAE/g) and flavonoid (51.47±0.60 mg RE/g) contents. This study indicated that B. dracunculifolia leaves extract has relevant in vivo hepatoprotective properties.
authors.affiliation: Identificação e Pesquisa em Princípios Ativos Naturais - NIPPAN, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n - Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil.
authors.forename: Túlio P
authors.initials: TP
authors.lastname: Rezende
authors.affiliation: Laboratório de Bioatividade Celular e Molecular, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n - Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil.
authors.forename: José Otávio
authors.initials: JO
authors.lastname: do A Corrêa
authors.affiliation: Laboratório de Imunopatologia e Patologia Experimental, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n - Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil.
authors.forename: Beatriz J V
authors.initials: BJ
authors.lastname: Aarestrup
authors.affiliation: Laboratório de Imunopatologia e Patologia Experimental, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n - Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil.
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Aarestrup
authors.affiliation: Laboratório de Farmacologia de Produtos Naturais, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n - Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil.
authors.forename: Orlando V
authors.initials: OV
authors.lastname: de Sousa
authors.affiliation: Identificação e Pesquisa em Princípios Ativos Naturais - NIPPAN, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n - Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil. ademar.alves@ufjf.edu.br.
authors.forename: Ademar A
authors.initials: AA
authors.lastname: da Silva Filho
mesh_terms: D000082:Acetaminophen / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000633:toxicity*; D000818:Animals; D031175:Baccharis / Q000737:chemistry*; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D004353:Drug Evaluation, Preclinical; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D017208:Rats, Wistar
chemical_list: D018712:Analgesics, Non-Narcotic; D016166:Free Radical Scavengers; D010936:Plant Extracts; D000082:Acetaminophen; D002251:Carbon Tetrachloride