title: Diverse families of antimicrobial peptides isolated from skin secretions of three species of East Asian frogs, Babina daunchina, Babina adenopleura, and Rana omeimontis (Ranidae).
abstract: Twenty-two novel cDNAs encoding 22 peptide precursors for 19 mature peptides including antimicrobial peptides (AMPs) were identified from East Asian frog species Babina daunchina, Babina adenopleura, and Rana omeimontis skin-derived cDNA libraries. Two atypical members of the brevinin-1 family AMPs, named brevinin-1AN1 (FLTGVLKLASKIPSVLCAVLKTC) and brevinin-1DN1(FLKGVINLASKIPSMLCAVLKTC), were purified from the skin secretions of B. adenopleura and B. daunchina, respectively. A member of the ranatuerin-2 family AMP named ranatuerin-2DN1 (GLFDSITQGLKDTAVKLLDKIKCKLSACPPA) was also purified from the skin secretion of B. daunchina. One AMP named japonicin-2OM1 (FIVPSIFLLKKAFCIALKKNC) was purified from the skin secretion of R. omeimontis. The antimicrobial tests showed that brevinin-1DN1, brevinin-1DN2, brevinin-1AN1, and japonicin-2OM1 possess higher antimicrobial activity against Gram-positive bacteria than Gram-negative bacteria.
authors.affiliation: 1 Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Hu
authors.forename: Shiqi
authors.initials: S
authors.lastname: Xu
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Hu
authors.forename: Chao
authors.initials: C
authors.lastname: Guo
authors.forename: Hao
authors.initials: H
authors.lastname: Meng
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jingze
authors.initials: J
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D008969:Molecular Sequence Data; D011898:Ranidae / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary
keywords: Ranidae; amphibia; brown frog; gene; peptide