title: Lyar, a cell growth-regulating zinc finger protein, was identified to be associated with cytoplasmic ribosomes in male germ and cancer cells.
abstract: Translational control is a basic mechanism for gene regulation in cells and important for tissue growth and development in mammals. Deregulation of the mechanism thus causes diseases such as cancer. Considering the importance of the ribosome as a factory of polypeptide synthesis, some new factors have been expected to be associated with the ribosome and involved in translational control. Our proteomic survey for these factors identified a zinc finger protein, Lyar, in cytoplasmic ribosomes of the rodent testis. Subcellular fractionation of the testis provided data supporting association of Lyar with ribosomes. Lyar was then suggested to be included in the 60S large subunit, but not in polysomes, by ultracentrifugation of testicular ribosomes. While analysis of tissue distribution of Lyar has indicated its testis-predominant expression, Lyar mRNA was expressed in the cancer cells originated from tissues other than testis, and Lyar promoted proliferation of NIH-3T3 cells. Furthermore, translation was increased by Lyar in vitro, pointing out the first experimental link between this protein and translation. Taken together, Lyar seems to be a new player in translational control and a potential target for cancer therapy.
authors.affiliation: Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601, Japan.
authors.forename: Kahori
authors.initials: K
authors.lastname: Yonezawa
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sugihara
authors.forename: Kenzi
authors.initials: K
authors.lastname: Oshima
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsuda
authors.forename: Daita
authors.initials: D
authors.lastname: Nadano
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D061986:MCF-7 Cells; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D014176:Protein Biosynthesis; D040901:Proteomics; D012270:Ribosomes / Q000378:metabolism*; D013737:Testis / Q000166:cytology* / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C000592018:LYAR protein, human; C081182:Lyar protein, mouse; D009687:Nuclear Proteins