title: [Effect of long-term fertilizing regime on soil microbial diversity and soil property].
abstract: OBJECTIVE
  To evaluate the effect of long-term fertilization on soil microbial community and soil chemical and physical properties.
  
  
  METHODS
  Using a high-throughput pyrosequencing technique, we studied microbial community in the 0-300 cm soil samples covering a 20-year field-experiment with different fertilization applications including inorganic fertilizer alone (N 300 kg/hm2, P2O5 150 kg/hm2 and K2O 60 kg/hm2) and inorganic fertilizer combined with straw (same application rate of N and P fertilizer combined with 5.4 t straw).
  
  
  RESULTS
  Actinobacteria and alpha-proteobacteria were the predominant groups in the topsoil (0-20 cm). As the soil depth increased, the relative abundance of actinobacteria decreased whereas that of proteobacteria, especially gamma-proteobacteria and beta-proteobacteria increased and gradually became the dominant groups in the subsoil (20-300 cm). Long-term fertilizing applications significantly affected soil microbial communities throughout the soil profile, and increased the relative abundance of ammonia-oxidizing archaea at 0-40 cm depth. In addition, agriculture management, e. g. irrigation may be an important driving factor for the distribution of ammonia-oxidizing bacteria in soil profile. Total nitrogen and organic carbon contents were the most influential factors on microbial community in the topsoil and in the subsoil, respectively.
  
  
  CONCLUSIONS
  Long-term fertilizer applications altered soil nutrient availability within the soil profile, which was likely to result in the different microbial community structure between the fertilizer treatments, especially for the subsoil.
authors.forename: Chenhua
authors.initials: C
authors.lastname: Li
authors.forename: Caixia
authors.initials: C
authors.lastname: Zhang
authors.forename: Lisong
authors.initials: L
authors.lastname: Tang
authors.forename: Zhengqin
authors.initials: Z
authors.lastname: Xiong
authors.forename: Baozhan
authors.initials: B
authors.lastname: Wang
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Jia
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000641:Ammonia / Q000032:analysis / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D044822:Biodiversity; D017753:Ecosystem; D005308:Fertilizers / Q000032:analysis*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D005308:Fertilizers; D012987:Soil; D000641:Ammonia