title: Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.
abstract: Olfactory sensory information is processed and integrated by circuits within the olfactory bulb (OB) before being sent to the olfactory cortex. In the mammalian OB, neural activity driven by external stimuli can lead to experience-dependent changes in structures and functions. In this study, quantitative proteomics techniques were employed to study proteome-wide changes in the OB under four levels of neural activity (from low to high): devoid of peripheral input (using a transgenic model), wild-type control, and short-term and long-term odor exposures. Our results revealed that proteins related to various processes were altered in the OBs of odor-deprived and odor-stimulated mice compared to the wild-type controls. These changes induced by odor stimulation were quite different from those induced by a deficit in peripheral olfactory inputs. Detailed analysis demonstrated that metabolic process and synaptic transmission were the most commonly altered pathways and that the effects of peripheral deprivation were more profound. Our comparative proteomics analysis indicated that olfactory deprivation and odor exposure lead to different alterations in the OB proteome, which provides new clues about the mechanisms underlying the olfactory deprivation- or odor stimulation-induced plasticity of OB function and organization.
  
  
  CONCLUSIONS
  By combining quantitative proteomics, bioinformatics and WB/IHC analysis, this study reports the results of the first comparative study on proteome-wide changes in the olfactory bulb under different levels of olfactory input. Odor deprivation and stimulation induced proteomic changes clearly demonstrate significant metabolic shifts and alterations on synaptic transmission. This quantitative system biology study leads to a new level of understanding in the development of olfactory bulb plasticity induced by odor deprivation or stimulation, and provides many new clues for the olfactory bulb related functional studies.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Hao-Long
authors.initials: HL
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Rao
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Lei-Ke
authors.initials: LK
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China. Electronic address: fuqiang.xu@wipm.ac.cn.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China. Electronic address: guol@whu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
mesh_terms: D000818:Animals; D051379:Mice; D018345:Mice, Knockout; D009473:Neuronal Plasticity / Q000502:physiology*; D009812:Odorants; D009830:Olfactory Bulb / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D020543:Proteome
keywords: Metabolic process; Odor deprivation; Odor exposure; Olfactory bulb; Quantitative proteomics; Synaptic transmission