title: Effective microbial calcite precipitation by a new mutant and precipitating regulation of extracellular urease.
abstract: Microbial calcite precipitation is a promising and environmental friendly biological technology in remediation of the surface and subsurface of porous media, especially for in situ soil remediation. The present study isolate a urea-degrading strain LH1 from soil on soybean root, identified as Bacillus niabensis strain (99% similarity) by 16S rRNA gene sequencing analysis. Then, using ultraviolet mutagenesis method, a mutant LHUM107 with outstanding urease-producing ability was further obtained to study its effects on calcite precipitation. The mutant LHUM107 had good genome stability and exhibited 92.2% urea-degrading efficiency till 21st generation. Response surface methodology (RSM) noted that the urea degradation was more dependent on initial urea addition, and brought forward the optimal conditions. Adapting to these optimal conditions, calcite precipitation by mutant LHUM107 and extracellular urease was respectively further investigated. It was shown that extracellular urease excreted from mutant LHUM107 was more effective and more targeted for CaCO3 precipitation.
authors.affiliation: Province Key Laboratory of Animal Resource and Epidemic Disease Prevention, School of Life Science, Liaoning University, Shenyang 110036, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Affiliated Hospital, Chengde Medical University, Chengde 067000, China.
authors.forename: Youxin
authors.initials: Y
authors.lastname: Song
authors.affiliation: Province Key Laboratory of Animal Resource and Epidemic Disease Prevention, School of Life Science, Liaoning University, Shenyang 110036, China.
authors.forename: Qijiu
authors.initials: Q
authors.lastname: Li
authors.affiliation: Province Key Laboratory of Animal Resource and Epidemic Disease Prevention, School of Life Science, Liaoning University, Shenyang 110036, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: He
authors.affiliation: Province Key Laboratory of Animal Resource and Epidemic Disease Prevention, School of Life Science, Liaoning University, Shenyang 110036, China; School of Environmental Science, Liaoning University, Shenyang 110036, China. Electronic address: ysong@lnu.edu.cn.
authors.forename: Youtao
authors.initials: Y
authors.lastname: Song
mesh_terms: D000704:Analysis of Variance; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D002119:Calcium Carbonate / Q000378:metabolism*; D011232:Chemical Precipitation; D005110:Extracellular Space / Q000201:enzymology*; D042822:Genomic Instability; D009154:Mutation / Q000235:genetics*; D010802:Phylogeny; D013696:Temperature; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000378:metabolism*
chemical_list: D014508:Urea; D014510:Urease; D002119:Calcium Carbonate
keywords: 16S rRNA gene sequencing; Bacillus; Extracellular urease; Microbial calcite precipitation; Urea-degrading