title: Neuroprotection of up-regulated carbon monoxide by electrical acupuncture on perinatal hypoxic-ischemic brain damage in rats.
abstract: This study investigated the neuroprotection and potential mechanism of carbon monoxide (CO) against perinatal hypoxic-ischemic brain damage in rats by electrical acupuncture (EA). Animal behavior, morphological changes, cystathionine beta-synthase (CBS), hypoxia-inducible factor-1α (HIF-1α), and heme oxygenase-1 (HO-1) expression levels, and CO content in rat cortex cells were determined. Results demonstrated that EA treatment decreased the slope behavior and increased the overhang behavior of perinatal rats. The treatment also decreased the number of positive cells. The activator and inhibitor of CBS aggravated and remitted the hypoxic damage in cortex cells, respectively. EA treatment decreased CBS expression level and increased HO-1 and HIF-1α expression levels in perinatal rat cortex cells. Compared with the control groups, the CO content of cortex cells in the EA treatment group significantly increased (**p < 0.01). We hypothesized that EA treatment increases cortical CO content to protect against hypoxic damage via the hydrogen sulfide/CBS-CO/HO-1-HIF-1α system. This study provided a significant reference for EA therapy and cued a novel protective mechanism for cerebral palsy.
authors.affiliation: Department of Pediatrics, General Hospital of Chinese People's Liberation Army, Beijing, 100853, China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Li
authors.forename: Xiuyu
authors.initials: X
authors.lastname: Shi
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Weiguang
authors.initials: W
authors.lastname: Li
authors.forename: Guofang
authors.initials: G
authors.lastname: Duan
authors.forename: Hualing
authors.initials: H
authors.lastname: Li
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Yang
authors.forename: Chenggang
authors.initials: C
authors.lastname: Zhang
authors.forename: Liping
authors.initials: L
authors.lastname: Zou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002248:Carbon Monoxide / Q000378:metabolism*; D002540:Cerebral Cortex / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D015671:Electroacupuncture; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
chemical_list: C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D002248:Carbon Monoxide; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat