title: [Preparation and immunogenicity of silk fibroin/chitosan microspheres for DNA vaccine delivery against infectious bursal disease virus].
abstract: To evaluate the immunities of biodegradable microsphere as a release delivery system for DNA vaccine against Infectious Bursal Disease Virus, in our study, silk fibroin/chitosan microsphere adjuvant was prepared with a precipitation/coacervation method. Both glutaraldehyde and Na2SO4 solution were used in cross-linking. No immune chicken were intramuscularly inoculated at 14 day-old and boosted 2 weeks later. The results show that glutaraldehyde destroyed the DNA activity of the vaccine whereas Na2SO4 solution did not. Factors of the chitosan concentration 0.5% (pH 5.0), silk fibroin concentration 0.6%, plasmid DNA (500 microg/mL) dissolved in 2% Na2SO4 solution were optimized to produce microsphere, with a loading capacity of 89.14%. The average particle size of SF-CS/pCI-VP2/4/3 microsphere is 1.98 microm, and it can protect the loading DNA vaccine from DNase I digestion. Data from anti IBDV ELISA antibodies in the serum show that immunization activity of the microsphere groups were generally higher than plasmid vaccine group (P < 0.05), and the SF/CS compound microspheres group was better than that of sole CS microsphere group. The developed SF/CS microspheres are a very promising vaccine delivery system.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Lv
authors.forename: Cun
authors.initials: C
authors.lastname: Zhang
authors.forename: Xingrong
authors.initials: X
authors.lastname: Zhu
authors.forename: Tuanyuan
authors.initials: T
authors.lastname: Shi
authors.forename: Shi
authors.initials: S
authors.lastname: Zhong
authors.forename: Zhiqi
authors.initials: Z
authors.lastname: Meng
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry; D000818:Animals; D018175:Birnaviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D002645:Chickens; D048271:Chitosan / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D007243:Infectious bursal disease virus; D008863:Microspheres; D010957:Plasmids; D011201:Poultry Diseases / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000737:chemistry*; D014765:Viral Vaccines / Q000737:chemistry*
chemical_list: D000276:Adjuvants, Immunologic; D019444:Vaccines, DNA; D014765:Viral Vaccines; D005349:Fibroins; D048271:Chitosan