title: Determination of iodate by HPLC-UV after on-line electrochemical reduction to iodide.
abstract: In this study, a novel on-line pre-column electrochemical instrument (PECI) coupled with high-performance liquid chromatography (HPLC) was developed, and a novel method based on PEC-HPLC-UV for amplifying the ultraviolet (UV) response of iodate (IO₃⁻) was studied. Iodate undergoes reduction in the PECI, and the resulting I(-) was injected to an HPLC system and detected by a UV detector. For IO₃⁻ analysis, conditions that can influence the reduction efficiency, including applied potential, pH value and salt concentration, were investigated in detail. In an appropriate condition, the UV response of iodate after passing through PECI was almost 10 times more than that of the initial form with good precision (relative standard deviation 2.0-4.3%). The detection limit and quantity limit were 9 and 20 ng, respectively. It can be concluded that the proposed method is simple and highly sensitive.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Beijing Techmate Technology Corporation Limited, Beijing 100070, People's Republic of China.
authors.forename: Weimei
authors.initials: W
authors.lastname: Lin
authors.affiliation: Beijing Techmate Technology Corporation Limited, Beijing 100070, People's Republic of China.
authors.forename: Xueliang
authors.initials: X
authors.lastname: Dai
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, People's Republic of China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Gao
authors.affiliation: Beijing Techmate Technology Corporation Limited, Beijing 100070, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, People's Republic of China qdqwzb@163.com.
authors.forename: Dongqin
authors.initials: D
authors.lastname: Quan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007452:Iodates / Q000032:analysis* / Q000737:chemistry; D007454:Iodides / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D012965:Sodium Chloride; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D007452:Iodates; D007454:Iodides; D012965:Sodium Chloride