title: Lactones from Ficus auriculata and their effects on the proliferation function of primary mouse osteoblasts in vitro.
abstract: Bioassay-guided fractionation of the petroleum ether, chloroform and EtOAc extracts of the stems of Ficus auriculata led to the isolation of five new 12-membered lactones (3R,4R)-4-hydroxy-de-O-methyllasiodiplodin (1), 6-oxolasiodiplodin (2) and ficusines A-C (3-5), together with three known related analogues (6-8). The structures of the new compounds were elucidated by comprehensive spectroscopic data. The absolute configurations of 3 and 8 were established by single crystal X-ray diffraction analysis. Compounds 3-5 represent the first 12-membered lactones with a quinone ring unit. Compounds 6 and 7 exhibited significant proliferation function of primary osteoblasts (OBs) in vitro. Especially, the promotion rate of 6 reached 151.55±1.34% (P<0.001) at the concentration of 100 μM.
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, PR China; School of Biochemical Engineering, Hainan Institute of Science and Technology, Haikou 571126, PR China.
authors.forename: Tai-Ming
authors.initials: TM
authors.lastname: Shao
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Cai-Juan
authors.initials: CJ
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, PR China. Electronic address: hchr116@hainnu.edu.cn.
authors.forename: Chang-Ri
authors.initials: CR
authors.lastname: Han
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, PR China. Electronic address: chgying123@163.com.
authors.forename: Guang-Ying
authors.initials: GY
authors.lastname: Chen
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, PR China; College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding 071002, PR China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Dai
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Song
authors.affiliation: College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding 071002, PR China.
authors.forename: Jin-Chao
authors.initials: JC
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Chen
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D030681:Ficus / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D013329:Structure-Activity Relationship
chemical_list: D007783:Lactones
keywords: Chemical constituents; Ficus auriculata; Lactones; Primary osteoblasts