title: [PPAR-γ agonist inhibits the expressions of chemokines induced by IFN-γ and TNF-α in renal tubular epithelial cells].
abstract: OBJECTIVE
  To investigate the effect of peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist 15d-PGJ2 on the expressions of CXCL9, CXCL10 and CXCL11 in renal tubular epithelial cells (HK-2) stimulated by interferon-γ (IFN-γ) plus tumor necrosis factor-α (TNF-α).
  
  
  METHODS
  HK-2 cells were stimulated with IFN-γ plus TNF-α only, or incubated with 15d-PGJ2 for 48 hours. CXCL9, CXCL10 and CXCL11 expressions were determined by real-time quantitative PCR (qRT-PCR) and ELISA.
  
  
  RESULTS
  IFN-γ plus TNF-α increased mRNA and protein expressions of CXCL9, CXCL10 and CXCL11 in HK-2 cells. PPAR-γ agonist 15d-PGJ2 inhibited the expressions of CXCL9, CXCL10 and CXCL11 in IFN-γ combined with TNF-α-induced HK-2 cells. Compared with the IFN-γ plus TNF-α group (untreated with 15d-PGJ2), the CXCL9, CXCL10 and CXCL11 were depressed by 76.8%, 78.7% and 81.9% at mRNA level and 66.9%, 86.6% and 39.9% at protein level in 2.0 ng/mL 15d-PGJ2-treated HK-2 cells, respectively (P<0.05).
  
  
  CONCLUSIONS
  PPAR-γ agonist 15d-PGJ2 could inhibit CXCL9, CXCL10 and CXCL11 production induced by IFN-γ combined with TNF-α in HK-2 cells.
authors.affiliation: Department of Clinical Laboratory, Affiliated People's Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Clinical Laboratory, Affiliated People's Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Fujian University of Tradition Chinese Medicine, Fuzhou 350004, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Clinical Laboratory, Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Urology, Fuzhou General Hospital of PLA, Nanjing Military Area Command, Fuzhou 350100, China.
authors.forename: Shunliang
authors.initials: S
authors.lastname: Yang
mesh_terms: D002460:Cell Line; D054357:Chemokine CXCL10 / Q000235:genetics / Q000378:metabolism; D054371:Chemokine CXCL11 / Q000235:genetics / Q000378:metabolism; D054370:Chemokine CXCL9 / Q000235:genetics / Q000378:metabolism; D018925:Chemokines / Q000235:genetics* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000166:cytology; D047495:PPAR gamma / Q000819:agonists* / Q000378:metabolism; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: C477819:15-deoxyprostaglandin J2; D054357:Chemokine CXCL10; D054371:Chemokine CXCL11; D054370:Chemokine CXCL9; D018925:Chemokines; D047495:PPAR gamma; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D015230:Prostaglandin D2