title: Automatic zebrafish heartbeat detection and analysis for zebrafish embryos.
abstract: A fully automatic detection and analysis method of heartbeats in videos of nonfixed and nonanesthetized zebrafish embryos is presented. This method reduces the manual workload and time needed for preparation and imaging of the zebrafish embryos, as well as for evaluating heartbeat parameters such as frequency, beat-to-beat intervals, and arrhythmicity. The method is validated by a comparison of the results from automatic and manual detection of the heart rates of wild-type zebrafish embryos 36-120 h postfertilization and of embryonic hearts with bradycardia and pauses in the cardiac contraction.
authors.affiliation: 1 Institute for Applied Computer Science (IAI) , Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany .
authors.forename: Christian
authors.initials: C
authors.lastname: Pylatiuk
authors.forename: Daniela
authors.initials: D
authors.lastname: Sanchez
authors.forename: Ralf
authors.initials: R
authors.lastname: Mikut
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Alshut
authors.forename: Markus
authors.initials: M
authors.lastname: Reischl
authors.forename: Sofia
authors.initials: S
authors.lastname: Hirth
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Rottbauer
authors.forename: Steffen
authors.initials: S
authors.lastname: Just
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000503:physiopathology*; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000502:physiology; D006339:Heart Rate; D014743:Videotape Recording / Q000295:instrumentation; D015027:Zebrafish / Q000196:embryology / Q000502:physiology*