title: Britanin Suppresses IgE/Ag-Induced Mast Cell Activation by Inhibiting the Syk Pathway.
abstract: The aim of this study was to determine whether britanin, isolated from the flowers of Inula japonica (Inulae Flos), modulates the generation of allergic inflammatory mediators in activated mast cells. To understand the biological activity of britanin, the authors investigated its effects on the generation of prostaglandin D2 (PGD2), leukotriene C4 (LTC4), and degranulation in IgE/Ag-induced bone marrow-derived mast cells (BMMCs). Britanin dose dependently inhibited degranulation and the generations of PGD2 and LTC4 in BMMCs. Biochemical analyses of IgE/Ag-mediated signaling pathways demonstrated that britanin suppressed the phosphorylation of Syk kinase and multiple downstream signaling processes, including phospholipase Cγ1 (PLCγ1)-mediated calcium influx, the activation of mitogen-activated protein kinases (MAPKs; extracellular signal-regulated kinase 1/2, c-Jun NH2-terminal kinase and p38), and the nuclear factor-κB (NF-κB) pathway. Taken together, the findings of this study suggest britanin suppresses degranulation and eicosanoid generation by inhibiting the Syk-dependent pathway and britanin might be useful for the treatment of allergic inflammatory diseases.
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China ; College of Pharmacy, Yeungnam University, Gyeongsan 712-749.
authors.forename: Yue
authors.initials: Y
authors.lastname: Lu
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan 712-749.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan 712-749.
authors.forename: Young Na
authors.initials: YN
authors.lastname: Park
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan 712-749.
authors.forename: Okyun
authors.initials: O
authors.lastname: Kwon
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan 712-749.
authors.forename: Donggen
authors.initials: D
authors.lastname: Piao
authors.affiliation: Department of Pathology, Catholic University of Daegu School of Medicine, Daegu 705-718.
authors.forename: Young-Chae
authors.initials: YC
authors.lastname: Chang
authors.affiliation: Department of Biological Science, Sungkyunkwan University, Suwon 440-746.
authors.forename: Cheorl-Ho
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Research and Development Division, Korean Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-210, Republic of Korea.
authors.forename: Eunkyung
authors.initials: E
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan 712-749.
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan 712-749.
authors.forename: Hyeun Wook
authors.initials: HW
authors.lastname: Chang
keywords: Allergic inflammation; Britanin; Degranulation; Eicosanoid; Mast cells; Mitogen-activated protein kinase; Syk kinase