title: Bionanoparticles as candidate reference materials for mobility analysis of nanoparticles.
abstract: We propose bionanoparticles as a candidate reference material for determining the mobility of nanoparticles over the range of 6 × 10(-8)-5 × 10(-6) m(2)V(-1)s(-1). Using an electrospray differential mobility analyzer (ES-DMA), we measured the empirical distribution of several bionanoparticles. All of them show monomodal distributions that are more than two times narrower than the currently used calibration particles for mobility larger than 6 × 10(-8) m(2)V(-1)s(-1) (diameters less than 60 nm). We also present a numerical method to calculate corrected distributions of bionanoparticles by separating the contribution of the diffusive transfer function. The corrected distribution is about 20% narrower than the empirical distributions. Even with the correction, the reduced width of the mobility distribution is about a factor of 2 larger than the diffusive transfer function. The additional broadening could result from the nonuniform conformation of bionanoparticles and from the presence of volatile impurities or solvent adducts. The mobilities of these investigated bionanoparticle are stable over a range of buffer concentration and molarity, with no evidence of temporal degradation over several weeks.
authors.affiliation: University of Maryland , College Park, Maryland 20742, United States.
authors.forename: R
authors.initials: R
authors.lastname: You
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Guha
authors.forename: G W
authors.initials: GW
authors.lastname: Mulholland
authors.forename: M R
authors.initials: MR
authors.lastname: Zachariah
mesh_terms: D002021:Buffers; D007136:Immunoglobulins / Q000737:chemistry; D017909:Levivirus / Q000737:chemistry; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014027:Tobacco Mosaic Virus / Q000737:chemistry
chemical_list: D002021:Buffers; D007136:Immunoglobulins; D012710:Serum Albumin, Bovine