title: Detection of antibodies to varicella-zoster virus in recipients of the varicella vaccine by using a luciferase immunoprecipitation system assay.
abstract: A high-throughput test to detect varicella-zoster virus (VZV) antibodies in varicella vaccine recipients is not currently available. One of the most sensitive tests for detecting VZV antibodies after vaccination is the fluorescent antibody to membrane antigen (FAMA) test. Unfortunately, this test is labor-intensive, somewhat subjective to read, and not commercially available. Therefore, we developed a highly quantitative and high-throughput luciferase immunoprecipitation system (LIPS) assay to detect antibody to VZV glycoprotein E (gE). Tests of children who received the varicella vaccine showed that the gE LIPS assay had 90% sensitivity and 70% specificity, a viral capsid antigen enzyme-linked immunosorbent assay (ELISA) had 67% and 87% specificity, and a glycoprotein ELISA (not commercially available in the United States) had 94% sensitivity and 74% specificity compared with the FAMA test. The rates of antibody detection by the gE LIPS and glycoprotein ELISA were not statistically different. Therefore, the gE LIPS assay may be useful for detecting VZV antibodies in varicella vaccine recipients. (This study has been registered at ClinicalTrials.gov under registration no. NCT00921999.).
authors.affiliation: Medical Virology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA jcohen@niaid.nih.gov.
authors.forename: Jeffrey I
authors.initials: JI
authors.lastname: Cohen
authors.affiliation: Medical Virology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Mir A
authors.initials: MA
authors.lastname: Ali
authors.affiliation: Department of Perioperative Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Bayat
authors.affiliation: Division of Pediatric Infectious Disease, Department of Pediatrics, Columbia University College of Physicians and Surgeons, New York, New York, USA.
authors.forename: Sharon P
authors.initials: SP
authors.lastname: Steinberg
authors.affiliation: Department of Microbiology, College of Medicine, Yeungnam University, Daegu, South Korea.
authors.forename: Hosun
authors.initials: H
authors.lastname: Park
authors.affiliation: Division of Pediatric Infectious Disease, Department of Pediatrics, Columbia University College of Physicians and Surgeons, New York, New York, USA.
authors.forename: Anne A
authors.initials: AA
authors.lastname: Gershon
authors.affiliation: Dental Clinical Research Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Burbelo
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D019433:Chickenpox Vaccine / Q000008:administration & dosage / Q000276:immunology*; D014645:Herpesvirus 3, Human / Q000276:immunology*; D057166:High-Throughput Screening Assays; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D008156:Luciferases / Q000032:analysis*; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019433:Chickenpox Vaccine; D014759:Viral Envelope Proteins; C056913:glycoprotein E, varicella-zoster virus; D008156:Luciferases