title: Stigmasterol Tetracosanoate, a New Stigmasterol Ester from the Egyptian Blepharis ciliaris.
abstract: A new stigmasterol ester: stigmasterol tetracosanoate (3), along with 7 compounds: β-sitosterol (1), stigmasterol (2), (2S,3S,4R)-2[(2'R)-2'-(hydroxyeicosanoyl amino) octadecane-1,3,4-triol (4), apigenin (5), β-sitosterol-3-O-β-D-glucopyranoside (6), stigmasterol-3-O-β-D-glucopyranose (7), and apigenin-7-O-β-D-glucopyranoside (8) were isolated from Blepharis ciliaris aerial parts. Compounds 1, 2, and 5-7 are reported here for the first time from the plant and 4 for the first time from the family. GCMS analysis revealed the presence of 45 fatty acids, 53 hydrocarbons, and 24 sterols. The different fractions exhibited mild cytotoxic in brine shrimp assay and anti-hyperglycaemic activities. The EtOAc fraction and TME (total MeOH extract) showed weak anti-malarial activity against P. falciparum. The CHCl3 fraction gave potent -anti-inflammatory activity compared with indomethacin.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
authors.forename: M A
authors.initials: MA
authors.lastname: El-Shanawany
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
authors.forename: H M
authors.initials: HM
authors.lastname: Sayed
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
authors.forename: S R M
authors.initials: SR
authors.lastname: Ibrahim
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
authors.forename: M A A
authors.initials: MA
authors.lastname: Fayed
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology; D000962:Antimalarials / Q000302:isolation & purification / Q000494:pharmacology; D004952:Esters / Q000302:isolation & purification* / Q000494:pharmacology*; D005227:Fatty Acids / Q000302:isolation & purification / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D051379:Mice; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D051381:Rats; D013265:Stigmasterol / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D000962:Antimalarials; D004952:Esters; D005227:Fatty Acids; D007004:Hypoglycemic Agents; D013265:Stigmasterol; C010210:lignoceric acid