title: Seroprevalence and major antigens recognized by sera from Trypanosoma cruzi-infected dogs from Jalisco, México.
abstract: Chagas disease is a major endemic disease caused by the protozoan parasite Trypanosoma cruzi. This parasitic disease is widely distributed throughout Latin America, affecting 10 million people. There are also reports of canine infection in the southern part of the United States. Dogs are considered the predominant domestic reservoir for T. cruzi in many areas of endemicity. In México, dog infection by this parasite has been poorly studied. In this work 209 dogs from six villages in Jalisco, México, were assessed to detect anti-T. cruzi antibodies by ELISA and Western blot. Seventeen (17) seropositive dogs (8.1 %) were detected by both tests, representing a seropositive value similar to that found in some southern states of México where the infection is present. No statistical differences were observed concerning the age and sex of infected and non-infected dogs. The major antigens recognized by positive sera were 26, 32, 66 and 80kDa. These proteins are candidates to develop a specific diagnostic method for canine Chagas. No antibodies against HSP16 protein were found in T. cruzi seropositive sera. This is the first report of canine serology of Chagas disease in this central part of México. This report will contribute to the knowledge of the infection status of domestic reservoirs in the state of Jalisco, México.
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Del. Coyoacán, México City, México.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Martínez
authors.affiliation: Área de Entomología Médica, Centro Universitario del Sur, Universidad de Guadalajara, Ciudad Guzmán, Jalisco, México.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martínez-Ibarra
authors.affiliation: Departamento de Biología Molecular, Instituto Nacional de Cardiología "Ignacio Chávez", México City, México.
authors.forename: Minerva
authors.initials: M
authors.lastname: Arce-Fonseca
authors.affiliation: Departamento de Biología Molecular, Instituto Nacional de Cardiología "Ignacio Chávez", México City, México.
authors.forename: Olivia
authors.initials: O
authors.lastname: Rodríguez-Morales
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Del. Coyoacán, México City, México.
authors.forename: Deyanira
authors.initials: D
authors.lastname: Pérez-Morales
authors.affiliation: Laboratorio de Inmunología Molecular y Proteómica, Dirección de Investigación, Instituto Nacional de Cardiología "Ignacio Chávez", México City, México.
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Reyes López
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Del. Coyoacán, México City, México. Electronic address: besgu@biomedicas.unam.mx.
authors.forename: Bertha
authors.initials: B
authors.lastname: Espinoza
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood*; D014355:Chagas Disease / Q000097:blood / Q000453:epidemiology / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood* / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D008800:Mexico / Q000453:epidemiology; D016036:Seroepidemiologic Studies; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan
keywords: Canine Chagas; Chagas canino; Infected dogs; Perros infectados; Trypanosoma cruzi