title: Transcriptional inhibition of etv2 expression is essential for embryonic cardiac development.
abstract: E-twenty six variant 2 (Etv2) transcription factor participates in cardiac, vascular-endothelial and blood cell lineage specification decisions during embryonic development. Previous studies have identified genomic elements in the etv2 locus responsible for vascular endothelial cell specification. Using transgenic analysis in zebrafish, we report here an etv2 proximal promoter fragment that prevents transgene misexpression in myocardial progenitor cells. This inhibition of etv2 expression in the cardiac progenitor population is partly mediated by Scl and Nkx2.5, likely through direct binding to the etv2 promoter, and cis-regulatory elements located in the first and second introns. The results identify an etv2 cis-regulatory mechanism controlling cardiovascular fate choice implying that etv2 participates in a transcriptional network mediating developmental plasticity of endothelial progenitor cells during embryonic development.
authors.affiliation: Medical College of Wisconsin, Department of Pediatrics, CRI Developmental Vascular Biology Program, Translational and Biomedical Research Center, CRI C3420, 8701 Watertown Plank Road, P.O. Box 26509, Milwaukee, WI 53226, USA.
authors.forename: Marcus-Oliver
authors.initials: MO
authors.lastname: Schupp
authors.affiliation: Division of Nephrology, Hypertension and Renal Transplantation, Room CG-98, 1600 Archer Road, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Waas
authors.affiliation: Medical College of Wisconsin, Department of Pediatrics, CRI Developmental Vascular Biology Program, Translational and Biomedical Research Center, CRI C3420, 8701 Watertown Plank Road, P.O. Box 26509, Milwaukee, WI 53226, USA; Division of Nephrology, Hypertension and Renal Transplantation, Room CG-98, 1600 Archer Road, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Chang-Zoon
authors.initials: CZ
authors.lastname: Chun
authors.affiliation: Medical College of Wisconsin, Department of Pediatrics, CRI Developmental Vascular Biology Program, Translational and Biomedical Research Center, CRI C3420, 8701 Watertown Plank Road, P.O. Box 26509, Milwaukee, WI 53226, USA. Electronic address: rramchan@mcw.edu.
authors.forename: Ramani
authors.initials: R
authors.lastname: Ramchandran
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D019070:Cell Lineage; D053595:Embryonic Stem Cells; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology / Q000196:embryology*; D004912:Erythrocytes / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006321:Heart / Q000196:embryology*; D000072576:Homeobox Protein Nkx-2.5; D060172:Morpholinos / Q000235:genetics; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*; D019076:Transgenes; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D000072576:Homeobox Protein Nkx-2.5; D060172:Morpholinos; D011518:Proto-Oncogene Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors; D029961:Zebrafish Proteins; C509408:Etv2 protein, zebrafish; C528182:Nkx2.5 protein, zebrafish; C489367:tal1 protein, zebrafish
keywords: Cardiac disc; Etv2; Scl; Transcription; Zebrafish