title: Lgr5 marks stem/progenitor cells in ovary and tubal epithelia.
abstract: The ovary surface epithelium (OSE) undergoes ovulatory tear and remodelling throughout life. Resident stem cells drive such tissue homeostasis in many adult epithelia, but their existence in the ovary has not been definitively proven. Lgr5 marks stem cells in multiple epithelia. Here we use reporter mice and single-molecule fluorescent in situ hybridization to document candidate Lgr5(+) stem cells in the mouse ovary and associated structures. Lgr5 is broadly expressed during ovary organogenesis, but becomes limited to the OSE in neonate life. In adults, Lgr5 expression is predominantly restricted to proliferative regions of the OSE and mesovarian-fimbria junctional epithelia. Using in vivo lineage tracing, we identify embryonic and neonate Lgr5(+) populations as stem/progenitor cells contributing to the development of the OSE cell lineage, as well as epithelia of the mesovarian ligament and oviduct/fimbria. Adult Lgr5(+) populations maintain OSE homeostasis and ovulatory regenerative repair in vivo. Thus, Lgr5 marks stem/progenitor cells of the ovary and tubal epithelia.
authors.affiliation: A-STAR Institute of Medical Biology, 8A Biomedical Grove, 06-06 Immunos, 138648, Singapore.
authors.forename: Annie
authors.initials: A
authors.lastname: Ng
authors.affiliation: A-STAR Institute of Medical Biology, 8A Biomedical Grove, 06-06 Immunos, 138648, Singapore.
authors.forename: Shawna
authors.initials: S
authors.lastname: Tan
authors.affiliation: A-STAR Institute of Medical Biology, 8A Biomedical Grove, 06-06 Immunos, 138648, Singapore.
authors.forename: Gurmit
authors.initials: G
authors.lastname: Singh
authors.affiliation: A-STAR Institute of Medical Biology, 8A Biomedical Grove, 06-06 Immunos, 138648, Singapore.
authors.forename: Pamela
authors.initials: P
authors.lastname: Rizk
authors.affiliation: A-STAR Institute of Medical Biology, 8A Biomedical Grove, 06-06 Immunos, 138648, Singapore.
authors.forename: Yada
authors.initials: Y
authors.lastname: Swathi
authors.affiliation: Cancer Science Institute of Singapore, National University of Singapore, 117599, Singapore.
authors.forename: Tuan Zea
authors.initials: TZ
authors.lastname: Tan
authors.affiliation: 1] Cancer Science Institute of Singapore, National University of Singapore, 117599, Singapore [2] Department of Obstetrics &Gynaecology, National University Hospital, 119228, Singapore.
authors.forename: Ruby Yun-Ju
authors.initials: RY
authors.lastname: Huang
authors.affiliation: A-STAR Institute of Medical Biology, 8A Biomedical Grove, 06-06 Immunos, 138648, Singapore.
authors.forename: Marc
authors.initials: M
authors.lastname: Leushacke
authors.affiliation: 1] A-STAR Institute of Medical Biology, 8A Biomedical Grove, 06-06 Immunos, 138648, Singapore [2] Centre for Regenerative Medicine, 47 Little France Crescent, University of Edinburgh, EH16 4TJ, UK [3] Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 117596, Singapore.
authors.forename: Nick
authors.initials: N
authors.lastname: Barker
mesh_terms: D053687:Adult Stem Cells / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D019070:Cell Lineage / Q000235:genetics; D053595:Embryonic Stem Cells / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005187:Fallopian Tubes / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D005819:Genetic Markers; D006706:Homeostasis / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D010053:Ovary / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D012038:Regeneration / Q000235:genetics; D059467:Transcriptome
chemical_list: D005819:Genetic Markers; C116884:LGR5 protein, human; C493597:Lgr5 protein, mouse; D043562:Receptors, G-Protein-Coupled