title: [The role of metallothionein and metal transporter on cadmium transport from mother to fetus].
abstract: We examined the role of Metallothionein (MT) and metal transporter on Cadmium (Cd) transport from mother to fetus. In this study, female Wistar rats were given Cd (as CdCl2) at a dose of 0, 1, 2 and 5 mg Cd/kg/d. Animals were sacrificed on the pregnant 19-21 d. The concentrations of Cd and MT, and gene expression of MT and metal transporters were determined in the placenta and in the fetuses. In mother rats, the concentration of Cd in the placenta increased dose-dependently, significantly. The Cd of MT-Cd form and the Cd of non-MT-Cd form increased also dose-dependently. In pathological examination of placenta tissue, syncytial trophoblast cells were damaged dose-dependently, particularly in 5 mg Cd/kg group. In fetuses, the Cd concentration in the total body, liver and kidney increased dose-dependently. The Cd concentration in liver of fetus was significantly higher than that in kidney. In gene expression of metal transporter, gene expression of Zip-8 and ATP7B increased dose-dependently in placenta in 0 mg, 1 mg and 2 mg groups, but the increase was not statistically significant. Based on these results, it was speculated that Cd in placenta is trapped to transport into fetus by MT in placenta. However, non-bound Cd by MT is leaked to fetus by damage of placenta tissue, and it was speculated that non-MT-Cd accumulated in liver of fetus. Namely, it was suggested the possibility that the non-bound Cd by MT is transported by metal transporter of zinc and copper, especially Zip-8 and ATP7B or leaked from the placenta as caused by damage of synsytial trophoblast.
authors.affiliation: Department of Environmental, Occupational Health and Toxicology, Graduate School of Medical Sciences, Kitasato University.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Fukase
authors.forename: Hisashi
authors.initials: H
authors.lastname: Tsugami
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ohba
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Ohta
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis*; D000818:Animals; D019256:Cadmium Chloride / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000096:biosynthesis*; D000073840:Copper-Transporting ATPases; D005260:Female; D008431:Maternal-Fetal Exchange / Q000502:physiology*; D008668:Metallothionein / Q000378:metabolism*; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy
chemical_list: D027682:Cation Transport Proteins; C561850:Slc39a8 protein, rat; D008668:Metallothionein; D000251:Adenosine Triphosphatases; C550619:Atp7b protein, rat; D000073840:Copper-Transporting ATPases; D019256:Cadmium Chloride