title: Prauserella coralliicola sp. nov., isolated from the coral Galaxea fascicularis.
abstract: A novel Gram-stain-positive actinobacterium, designated strain SCSIO 11529(T), was isolated from tissues of the stony coral Galaxea fascicularis, and characterized by using a polyphasic approach. The temperature range for growth was 22-50 °C (optimum 28-45 °C), the pH range for growth was 6.0-8.0 (optimum pH 7.0), and the NaCl concentration range for growth was 0-7% (w/v) NaCl. The polar lipid profile contained diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and an unknown polar lipid. The predominant menaquinone was MK-9(H4). The major fatty acids (>10%) were iso-C16:0, iso-C17:1ω6c, iso-C16:1 H and C16:1ω7c/iso-C15:0 2-OH. The DNA G+C content of strain SCSIO 11529(T) was 70.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCSIO 11529(T) belongs to the genus Prauserella, with the closest neighbours being Prauserella marina MS498(T) (97.0% 16S rRNA gene sequence similarity), Prauserella rugosa DSM 43194(T) (96.4%) and Prauserella flava YIM 90630(T) (95.9%). Based on the evidence of the present study, strain SCSIO 11529(T) is considered to represent a novel species of the genus Prauserella, for which the name Prauserella coralliicola sp. nov. is proposed. The type strain is SCSIO 11529(T) ( = DSM 45821(T) = NBRC 109418(T)).
authors.affiliation: Graduate University of CAS, Beijing, 100049, PR China.|CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, PR China.
authors.forename: Jia-Fa
authors.initials: JF
authors.lastname: Wu
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Liao Cheng Hospital, 67 West Dongchang Road, Liao Cheng, 252000, PR China.
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: You
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, PR China.
authors.forename: Si
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D057894:Coral Reefs; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9