title: Melanoma tumors alter proinflammatory cytokine production and monoamine brain function, and induce depressive-like behavior in male mice.
abstract: Depression is a commonly observed disorder among cancer patients; however, the mechanisms underlying the relationship between these disorders are not well known. We used an animal model to study the effects of tumor development on depressive-like behavior manifestation, proinflammatory cytokine expression, and central monoaminergic activity. Male OF1 mice were inoculated with B16F10 melanoma tumor cells and subjected to a 21-day behavioral evaluation comprising the novel palatable food (NPF) test and tail suspension test (TST). The mRNA expression levels of proinflammatory cytokines, interleukin (IL)-1β and IL-6, and tumor necrosis factor-alpha (TNF-α), were measured in the hypothalamus and hippocampus and the levels of IL-6 and TNF-α were measured in the blood plasma. We similarly determined the monoamine turnover in various brain areas. The tumors resulted in increasing the immobility in TST and the expression level of IL-6 in the hippocampus. These increases corresponded with a decrease in dopaminergic activity in the striatum and a decrease in serotonin turnover in the prefrontal cortex. Similarly, a high level of tumor development produced increases in the brain expression levels of IL-6 and TNF-α and plasma levels of IL-6. Our findings suggest that these alterations in inflammatory cytokines and monoaminergic system function might be responsible for the manifestation of depressive-like behaviors in tumor-bearing mice.
authors.affiliation: Department of Basic Psychological Processes and Their Development, Basque Country University, Avda. Tolosa 70, 20018 San Sebastián, Spain.
authors.forename: Andrea
authors.initials: A
authors.lastname: Lebeña
authors.affiliation: Department of Basic Psychological Processes and Their Development, Basque Country University, Avda. Tolosa 70, 20018 San Sebastián, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Vegas
authors.affiliation: Department of Basic Psychological Processes and Their Development, Basque Country University, Avda. Tolosa 70, 20018 San Sebastián, Spain.
authors.forename: Eneritz
authors.initials: E
authors.lastname: Gómez-Lázaro
authors.affiliation: Department of Basic Psychological Processes and Their Development, Basque Country University, Avda. Tolosa 70, 20018 San Sebastián, Spain.
authors.forename: Amaia
authors.initials: A
authors.lastname: Arregi
authors.affiliation: Department of Basic Psychological Processes and Their Development, Basque Country University, Avda. Tolosa 70, 20018 San Sebastián, Spain.
authors.forename: Larraitz
authors.initials: L
authors.lastname: Garmendia
authors.affiliation: Department of Basic Psychological Processes and Their Development, Basque Country University, Avda. Tolosa 70, 20018 San Sebastián, Spain.
authors.forename: Garikoitz
authors.initials: G
authors.lastname: Beitia
authors.affiliation: Department of Basic Psychological Processes and Their Development, Basque Country University, Avda. Tolosa 70, 20018 San Sebastián, Spain. Electronic address: a.azpiroz@ehu.es.
authors.forename: Arantza
authors.initials: A
authors.lastname: Azpiroz
mesh_terms: D059445:Anhedonia / Q000502:physiology; D000818:Animals; D021461:Animals, Outbred Strains; D001921:Brain / Q000503:physiopathology*; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D003866:Depressive Disorder / Q000209:etiology / Q000503:physiopathology*; D004298:Dopamine / Q000378:metabolism; D005247:Feeding Behavior / Q000502:physiology; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008545:Melanoma / Q000150:complications / Q000503:physiopathology*; D051379:Mice; D009368:Neoplasm Transplantation; D009483:Neuropsychological Tests; D011897:Random Allocation; D012701:Serotonin / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; D012701:Serotonin; D004298:Dopamine
keywords: Anhedonia; Cytokines; Inflammation; Melanoma tumor; Monoamines; Tail suspension test