title: Dachsous-dependent asymmetric localization of spiny-legs determines planar cell polarity orientation in Drosophila.
abstract: In Drosophila, planar cell polarity (PCP) molecules such as Dachsous (Ds) may function as global directional cues directing the asymmetrical localization of PCP core proteins such as Frizzled (Fz). However, the relationship between Ds asymmetry and Fz localization in the eye is opposite to that in the wing, thereby causing controversy regarding how these two systems are connected. Here, we show that this relationship is determined by the ratio of two Prickle (Pk) isoforms, Pk and Spiny-legs (Sple). Pk and Sple form different complexes with distinct subcellular localizations. When the amount of Sple is increased in the wing, Sple induces a reversal of PCP using the Ds-Ft system. A mathematical model demonstrates that Sple is the key regulator connecting Ds and the core proteins. Our model explains the previously noted discrepancies in terms of the differing relative amounts of Sple in the eye and wing.
authors.affiliation: Research Center for Biosignal, Akita University, Akita 010-8543, Japan; Department of Cell Biology and Morphology, Akita University Graduate School of Medicine, Akita 010-8543, Japan; Global COE program, Gunma University and Akita University, Akita 010-8543, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Ayukawa
authors.affiliation: Research Institute for Electronic Science, Hokkaido University, Hokkaido 060-0812, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Akiyama
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna 1030, Austria.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Mummery-Widmer
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna 1030, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Stoeger
authors.affiliation: Department of Medical Biology, Akita University Graduate School of Medicine, Akita 010-8543, Japan; Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Tokyo 102-0075, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Sasaki
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna 1030, Austria.
authors.forename: Juergen A
authors.initials: JA
authors.lastname: Knoblich
authors.affiliation: Department of Cell Biology and Morphology, Akita University Graduate School of Medicine, Akita 010-8543, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Senoo
authors.affiliation: Research Center for Biosignal, Akita University, Akita 010-8543, Japan; Global COE program, Gunma University and Akita University, Akita 010-8543, Japan; Department of Medical Biology, Akita University Graduate School of Medicine, Akita 010-8543, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Sasaki
authors.affiliation: Research Center for Biosignal, Akita University, Akita 010-8543, Japan; Department of Cell Biology and Morphology, Akita University Graduate School of Medicine, Akita 010-8543, Japan; Global COE program, Gunma University and Akita University, Akita 010-8543, Japan. Electronic address: yamazaki@med.akita-u.ac.jp.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Yamazaki
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D016764:Cell Polarity; D054910:Compound Eye, Arthropod / Q000166:cytology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008957:Models, Genetic; D009928:Organ Specificity; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D014921:Wings, Animal / Q000166:cytology / Q000378:metabolism
chemical_list: D015820:Cadherins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D060588:LIM Domain Proteins; D020033:Protein Isoforms; C094449:ds protein, Drosophila; C121600:pk protein, Drosophila