title: Phototherapeutic functionality of biocompatible graphene oxide/dendrimer hybrids.
abstract: Hydroxyl-terminated fourth generation poly(amido amine) dendrimer and folic acid were chemically bound on graphene oxide. The resultant hybrids exhibited one-photon and two-photon fluorescence emission, since the excitation irradiation at 390 and 780nm on the hybrids brought a fluorescence emission in the visible region around 450nm. In addition, the photocytotoxicity study revealed that under the two-photon excitation at 780nm, the hybrids can absorb near-infrared light and generate reactive oxygen species which can oxidize the HeLa cells and cause their death, suggesting the phototherapeutic behavior. Cytotoxicity measurement revealed the high biocompatibility of the hybrids toward HeLa cells. Thus, the present biocompatible hybrids consisting of only dendrimer, folic acid and graphene oxide have potentials as photodynamic therapeutic agents for medical treatment.
authors.affiliation: Graduate Institute of Applied Science and Technology, 43 Section 4, Keelung Road, Taipei 10607, Taiwan.
authors.forename: Ampornphan
authors.initials: A
authors.lastname: Siriviriyanun
authors.affiliation: Graduate Institute of Applied Science and Technology, 43 Section 4, Keelung Road, Taipei 10607, Taiwan; Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Section 4, Keelung Road, Taipei 10607, Taiwan. Electronic address: imae@mail.ntust.edu.tw.
authors.forename: Toyoko
authors.initials: T
authors.lastname: Imae
authors.affiliation: Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), CIBER en Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), C/Jordi Girona 18-26, 08034 Barcelona, Spain.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Calderó
authors.affiliation: Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), CIBER en Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), C/Jordi Girona 18-26, 08034 Barcelona, Spain.
authors.forename: Conxita
authors.initials: C
authors.lastname: Solans
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D050091:Dendrimers / Q000494:pharmacology*; D005453:Fluorescence; D005492:Folic Acid / Q000494:pharmacology; D006108:Graphite / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D017785:Photons; D010789:Phototherapy
chemical_list: D001672:Biocompatible Materials; D050091:Dendrimers; D006108:Graphite; D005492:Folic Acid
keywords: Fluorescence emission; Graphene oxide; Hydroxy-terminated fourth generation poly(amido amine) dendrimer; Reactive oxygen species; Two-photon excitation