title: Phytochemical analysis of ethanolic extract of Dichrostachys Cinerea W and Arn leaves by a thin layer chromatography, high performance thin layer chromatography and column chromatography.
abstract: BACKGROUND
  The leaves of Dichrostachys cinerea are used as laxative, diuretic, painkiller. It is also used in the treatment of gonorrhoea, boils, oedema, gout, veneral diseases and nasopharyngeal affections, etc.
  
  
  METHODS
  The Phytochemical investigation of ethanolic extract of D. cinerea leaves were performed by standard chemical tests, thin layer chromatography (TLC) by using various solvent systems, and by high performance liquid chromatography (HPTLC). Two compounds were isolated by column chromatography and one of the compounds was identified by various spectral studies.
  
  
  RESULTS
  Preliminary phytochemical screening of ethanolic extract of D. cinerea leaves showed the presence of Carbohydrates, proteins, Glycosides, Saponins, Tannins, Aminoacids and Terpenoids. The TLC and HPTLC fingerprint of ethanolic extract were studied and various fractions were isolated by column chromatography and one of the fraction contain β-amyrin glucoside which was confirmed by Infra Red[IR] Spectroscopy, (1)H-Nuclear Magnetic Resonance (NMR), C-(13) NMR and Mass spectroscopic (MS) studies.
authors.affiliation: Department of Pharmaceutical Technology, Anna University, Bharathidasan Institute of Technology, Tiruchirappalli, India.
authors.forename: M
authors.initials: M
authors.lastname: Vijayalakshmi
authors.affiliation: Department of Pharmacognosy, Madurai Medical College, Madurai, India.
authors.forename: K
authors.initials: K
authors.lastname: Periyanayagam
authors.affiliation: Department of Pharmaceutical Technology, Anna University, Bharathidasan Institute of Technology, Tiruchirappalli, India.
authors.forename: K
authors.initials: K
authors.lastname: Kavitha
authors.affiliation: Department of Pharmaceutical Technology, Anna University, Bharathidasan Institute of Technology, Tiruchirappalli, India.
authors.forename: K
authors.initials: K
authors.lastname: Akilandeshwari
keywords: Column chromatography; Dichrostachys cinerea; high performance thin layer chromatography; leaves extract; phytochemical evaluation