title: [Antioxidative effects of cysteinyl leukotriene receptor antagonists montelukast and HAMI 3379 on ischemic injury in rat cortical neurons in vitro].
abstract: To investigate the antioxidative effects of two cysteinyl leukotriene receptors antagonists (CysLT1R and CysLT2R) montelukast and HAMI 3379 on ischemic injury of rat cortical neurons in vitro.
  
  
  
  Cultured rat cortical neurons were pretreated with CysLT1R antagonist montelukast and CysLT2R antagonist HAMI 3379, and then exposed to oxygen-glucose deprivation/recovery (OGD/R）or H2O2. Reactive oxygen species (ROS) mitochondrial membrane potential (MMP) depolarization, neuronal viability and lactate dehydrogenase (LDH) release were determined. Meanwhile, RNA interference was used to inhibit the expression of CysLT1R and CysLT2R，and the effects were observed.
  
  
  
  ROS production in neurons was significantly increased after 1 h OGD, which reached the peak at 30 min and lasted for 1.5 h after recovery. Montelukast and HAMI 3379 at 0.01-1μmol/L moderately decreased OGD/R-induced ROS production (P<0.05). Montelukast mildly attenuated OGD/R-induced MMP depolarization (P<0.05)，but HAMI 3379 had no effect. H2O2 reduced neuronal viability and increased LDH release, namely inducing neuronal injury. Montelukast and HAMI 3379 at 0.1-1μmol/L moderately attenuated H2O2-induced neuronal injury (P<0.05). However, both CysLT1R siRNA and CysLT2R shRNA did not significantly affect the responses mentioned above.
  
  
  
  In ischemic neuronal injury, montelukast and HAMI 3379 exert a moderate antioxidative effect, and this effect may be receptor-independent.
authors.affiliation: Department of Pharmacology，Zhejiang University School of Medicine，Hangzhou 310058，China.
authors.forename: Dong-min
authors.initials: DM
authors.lastname: Xu
authors.affiliation: Department of Pharmacology，Zhejiang University School of Medicine，Hangzhou 310058，China.
authors.forename: Xia-yan
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology，Zhejiang University School of Medicine，Hangzhou 310058，China.
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Wang
authors.affiliation: Department of Pharmacology,School of Basic Medicine, Hangzhou Normal University;Hangzhou Key Laboratory of Neurobiology, Hangzhou 310036,China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, School of Basic Medicine, Hangzhou Normal University; Hangzhou Key Laboratory of Neurobiology, Hangzhou 310036, China.
authors.forename: Li-hui
authors.initials: LH
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology，Zhejiang University School of Medicine，Hangzhou 310058，China.
authors.forename: Qiao-juan
authors.initials: QJ
authors.lastname: Shi
authors.affiliation: Department of Pharmacology，Zhejiang University School of Medicine，Hangzhou 310058，China.
authors.forename: San-hua
authors.initials: SH
authors.lastname: Fang
authors.affiliation: Department of Pharmacology，Zhejiang University School of Medicine，Hangzhou 310058，China.
authors.forename: Yun-bi
authors.initials: YB
authors.lastname: Lu
authors.affiliation: Department of Pharmacology，Zhejiang University School of Medicine，Hangzhou 310058，China.
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology，Zhejiang University School of Medicine，Hangzhou 310058，China.
authors.forename: Er-qing
authors.initials: EQ
authors.lastname: Wei
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015687:Cell Hypoxia / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology*; D003521:Cyclopropanes; D020024:Leukotriene Antagonists / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010795:Phthalic Acids / Q000494:pharmacology*; D011804:Quinolines / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013440:Sulfides
chemical_list: C549635:3-(((3-carboxycyclohexyl)amino)carbonyl)-4-(3-(4-(4-(cyclohexyloxy)butoxy)phenyl)propoxy)benzoic acid; D000085:Acetates; D000975:Antioxidants; D003509:Cyclohexanecarboxylic Acids; D003521:Cyclopropanes; D020024:Leukotriene Antagonists; D010795:Phthalic Acids; D011804:Quinolines; D017382:Reactive Oxygen Species; D013440:Sulfides; C093875:montelukast