title: Autologous fat graft and bone marrow-derived mesenchymal stem cells assisted fat graft for treatment of Parry-Romberg syndrome.
abstract: BACKGROUND
  Progressive facial hemiatrophy, also called Parry-Romberg syndrome (PRS), is characterized by slowly progressive atrophy of one side of the face and primarily involves the subcutaneous tissue and fat. The restoration of facial contour and symmetry in patients affected by PRS still remains a challenge clinically. Fat graft is a promising treatment but has some shortcomings, such as unpredictability and low rate of graft survival due to partial necrosis. To obviate these disadvantages, fat graft assisted by bone marrow-derived mesenchymal stem cells (BMSCs) was used to treat PRS patients and the outcome was evaluated in comparison with the conventional treatment by autologous fat graft.
  
  
  METHODS
  Autologous fat graft was harvested by tumescent liposuction. Bone marrow-derived mesenchymal stem cells were then isolated by human Lymphocytes Separation Medium through density gradient centrifugation. Twenty-six patients were treated with autologous fat graft only (group A), whereas 10 other patients were treated with BMSC-assisted fat graft (group B). The Coleman technique was applied in all fat graft injections.
  
  
  RESULTS
  The follow-up period was 6 to 12 months in this study, In group A, satisfactory outcome judged by symmetrical appearances was obtained with 1 injection in 12 patients, 2 injections in 8 patients, and 3 injections in 4 patients. However, the result of 1 patient was not satisfactory and 1 patient was overcorrected. In group B, 10 patients obtained satisfactory outcomes and almost reached symmetry by 1 injection. No complications (infection, hematoma, or subcutaneous mass) were observed.
  
  
  CONCLUSIONS
  The results suggest that BMSC-assisted fat graft is effective and safe for soft tissue augmentation and may be superior to conventional lipoinjection. Additional study is necessary to further evaluate the efficacy of this technique.
authors.affiliation: *Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi; and †Department of Plastic Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jianhui
authors.forename: Yi
authors.initials: Y
authors.lastname: Chenggang
authors.forename: Lu
authors.initials: L
authors.lastname: Binglun
authors.forename: Han
authors.initials: H
authors.lastname: Yan
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Ma
authors.initials: M
authors.lastname: Xianjie
authors.forename: Su
authors.initials: S
authors.lastname: Yingjun
authors.forename: Guo
authors.initials: G
authors.lastname: Shuzhong
mesh_terms: D000273:Adipose Tissue / Q000637:transplantation*; D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells; D005150:Facial Hemiatrophy / Q000601:surgery*; D005260:Female; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D019651:Plastic Surgery Procedures / Q000379:methods; D014182:Transplantation, Autologous; D055815:Young Adult