title: Malignancy Associated MicroRNA Expression Changes in Canine Mammary Cancer of Different Malignancies.
abstract: MicroRNA has been suspected to be generally involved in carcinogenesis since their first description. A first study supported this assumption for canine mammary tumors when miRNA expression was compared to normal gland. The present study extends these results by comparing the expression of 16 microRNA (miRNA) and 4 small nucleolar RNA (snoRNA) in tumors of different malignancy, for example, adenomas, nonmetastasizing and metastasizing carcinomas as well as lymph node metastases, with each other and with normal mammary gland. All neoplastic tissues differed in their miR-210 expression levels from normal gland. While metastatic cells differed in their expression of mir-29b, miR-101, mir-125a, miR-143, and miR-145 from primary tumors, the comparison of miRNA expression in primary tumors of different malignancy failed to reveal significant differences except for a significant downregulation of mir-125a in metastasizing carcinomas when compared to adenomas.
authors.affiliation: Institute of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Straße 15, 14163 Berlin, Germany.
authors.forename: Marie-Charlotte
authors.identifier: 0000-0002-9463-7066
authors.initials: MC
authors.lastname: von Deetzen
authors.affiliation: Molecular Pulmonology, German Center for Lung Research, Philipps Universität Marburg, Hans-Meerwein-Straße 2, 35043 Marburg, Germany.
authors.forename: Bernd T
authors.initials: BT
authors.lastname: Schmeck
authors.affiliation: Institute of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Straße 15, 14163 Berlin, Germany.
authors.forename: Achim D
authors.initials: AD
authors.lastname: Gruber
authors.affiliation: Institute of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Straße 15, 14163 Berlin, Germany.
authors.forename: Robert
authors.identifier: 0000-0002-6308-0568
authors.initials: R
authors.lastname: Klopfleisch