title: Serum oxidative stress markers and lipidomic profile to detect NASH patients responsive to an antioxidant treatment: a pilot study.
abstract: Liver steatosis can evolve to steatohepatitis (NASH) through a series of biochemical steps related to oxidative stress in hepatocytes. Antioxidants, such as silybin, have been proposed as a treatment of patients with nonalcoholic fatty liver disease (NAFLD) and NASH. In this study, we evaluated, in patients with histologically documented NASH, the oxidant/antioxidant status and lipid "fingerprint" in the serum of NASH patients, both in basal conditions and after 12 months of treatment with silybin-based food integrator Realsil (RA). The oxidant/antioxidant status analysis showed the presence of a group of patients with higher basal severity of disease (NAS scores 4.67 ± 2.5) and a second group corresponding to borderline NASH (NAS scores = 3.8 ± 1.5). The chronic treatment with RA changed the NAS score in both groups that reached the statistical significance only in group 2, in which there was also a significant decrease of serum lipid peroxidation. The lipidomic profile showed a lipid composition similar to that of healthy subjects with a restoration of the values of free cholesterol, lysoPC, SM, and PC only in group 2 of patients after treatment with RA. Conclusion. These data suggest that lipidomic and/or oxidative status of serum from patients with NASH could be useful as prognostic markers of response to an antioxidant treatment.
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via De Crecchio 7, 80138 Naples, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Stiuso
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via De Crecchio 7, 80138 Naples, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Scognamiglio
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via De Crecchio 7, 80138 Naples, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Murolo
authors.affiliation: Department of Agriculture, University Federico II of Naples, Parco Gussone, Naples, 80055 Portici, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Ferranti
authors.affiliation: Department of Agriculture, University Federico II of Naples, Parco Gussone, Naples, 80055 Portici, Italy.
authors.forename: Carmela
authors.initials: C
authors.lastname: De Simone
authors.affiliation: Department of Medical, Surgical, Neurological, Metabolic and Geriatric Sciences, Second University of Naples, Piazza Miraglia 2, 80138 Naples, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Rizzo
authors.affiliation: Department of Clinical and Experimental Medicine, Interuniversity Research Centre on Food, Nutrition and Gastrointestinal Tract (CIRANAD), Second University of Naples, via Pansini 5, 80131 Naples, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Tuccillo
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Via De Crecchio 7, 80138 Naples, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Caraglia
authors.affiliation: Department of Clinical and Experimental Medicine, Interuniversity Research Centre on Food, Nutrition and Gastrointestinal Tract (CIRANAD), Second University of Naples, via Pansini 5, 80131 Naples, Italy.
authors.forename: Carmelina
authors.initials: C
authors.lastname: Loguercio
authors.affiliation: Department of Clinical and Experimental Medicine, Interuniversity Research Centre on Food, Nutrition and Gastrointestinal Tract (CIRANAD), Second University of Naples, via Pansini 5, 80131 Naples, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Federico
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D055432:Metabolomics; D009569:Nitric Oxide / Q000097:blood; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood* / Q000175:diagnosis* / Q000188:drug therapy; D018384:Oxidative Stress / Q000187:drug effects; D010713:Phosphatidylcholines / Q000378:metabolism; D010865:Pilot Projects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010713:Phosphatidylcholines; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D002374:Catalase; D013482:Superoxide Dismutase