title: Antimutagenic and antigenotoxic potential of grape juice concentrate in blood and liver of rats exposed to cadmium.
abstract: The aim of this study was to evaluate the antimutagenic and antigenotoxic potential of grape juice concentrate in rodent organs exposed to cadmium chloride intoxication. A total of 15 Wistar rats were distributed into three groups (n = 5), as follows: control group (CTRL; nontreated group), cadmium group (Cd), and cadmium-grape juice group (Cd + GJ). Exposed animals received intraperitoneal injection of cadmium chloride (1.2 mg/kg body weight) diluted in water and, after 15 days, Cd + GJ group received grape juice concentrate for 15 days, by gavage (0.8 mL, 1.18 mg of polyphenols kg(-1) day(-1)). Grape juice concentrate was able to decrease genotoxic effects induced by cadmium in peripheral blood and liver cells as depicted by single cell gel (comet) and micronucleus assays. A decrease for anti-8-hydroxy-20-deoxyguanosine (8OHdG) expression in hepatocytes of animals exposed to cadmium and treated with grape juice concentrate was also detected. Higher CuZn-SOD activity was observed in liver cells of the Cd + GJ group. No remarkable differences were seen regarding Mn-SOD activity among groups. Taken together, our results demonstrate that grape juice concentrate was able to exert antimutagenic and antigenotoxic activities in blood and liver cells of rats exposed to cadmium.
authors.affiliation: Department of Pathology, Federal University of Sao Paulo, UNIFESP, São Paulo, Brazil.
authors.forename: Carolina Foot Gomes
authors.initials: CF
authors.lastname: de Moura
authors.forename: Flávia Andressa Pidone
authors.initials: FA
authors.lastname: Ribeiro
authors.forename: Gustavo Protasio Pacheco
authors.initials: GP
authors.lastname: de Jesus
authors.forename: Victor Hugo Pereira
authors.initials: VH
authors.lastname: da Silva
authors.forename: Celina Tizuko Fujiyama
authors.initials: CT
authors.lastname: Oshima
authors.forename: Andréa Pittelli Boiago
authors.initials: AP
authors.lastname: Gollücke
authors.forename: Odair
authors.initials: O
authors.lastname: Aguiar
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001628:Beverages; D019256:Cadmium Chloride / Q000633:toxicity*; D004249:DNA Damage; D004785:Environmental Pollutants / Q000633:toxicity*; D005638:Fruit / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*
chemical_list: D016587:Antimutagenic Agents; D004785:Environmental Pollutants; D010936:Plant Extracts; D013482:Superoxide Dismutase; D019256:Cadmium Chloride