title: Maternal smoking does not affect the amount of cell-free fetal DNA in maternal plasma during the 1st trimester of pregnancy.
abstract: CffDNA, from 344 non-smoking, 38 smoking and 33 ex-smoking pregnant women at 11 (+0)-13 (+6) gestational weeks, was extracted and quantified by the multicopy DYS14, as the fetal DNA marker and using the quantitative real-time PCR 7300 detection system. The smoking habit was based on maternal self-report, confirmed by cotinine levels and male fetuses were verified by phenotype at birth. The genders of newborns were compared with DYS14-cffDNA analysis, achieving a 100% diagnostic accuracy of the test. A total of 177 non-smokers, 18 smokers and 22 ex-smoker pregnancies with male fetuses were identified by the cffDNA concentration. Results showed that smoking status was not associated with different amounts of DYS14-cffDNA (p = 0.159), suggesting the possibility of offering cffDNA testing to all pregnant women, even if they are active smokers or ex-smokers, and the test can be unadjusted for smoking status.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Perugia , Perugia , Italy.
authors.forename: F
authors.initials: F
authors.lastname: Tarquini
authors.forename: E
authors.initials: E
authors.lastname: Picchiassi
authors.forename: M
authors.initials: M
authors.lastname: Centra
authors.forename: L
authors.initials: L
authors.lastname: Pennacchi
authors.forename: F
authors.initials: F
authors.lastname: Galeone
authors.forename: V
authors.initials: V
authors.lastname: Bini
authors.forename: G C
authors.initials: GC
authors.lastname: Di Renzo
authors.forename: G
authors.initials: G
authors.lastname: Coata
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004247:DNA / Q000097:blood*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000097:blood*; D012907:Smoking / Q000097:blood*
chemical_list: D004247:DNA
keywords: cffDNA; maternal smoking; non-invasive prenatal test