title: Warifteine, an alkaloid purified from Cissampelos sympodialis, inhibits neutrophil migration in vitro and in vivo.
abstract: Cissampelos sympodialis Eichl is a plant from the Northeast and Southeast of Brazil. Its root infusion is popularly used for treatment of inflammatory and allergic diseases. We investigated whether warifteine, its main alkaloid, would have anti-inflammatory effect due to a blockage of neutrophil function. In vivo warifteine treatment inhibited casein-induced neutrophil migration to the peritoneal cavity but did not inhibit neutrophil mobilization from the bone marrow. Analysis of the direct effect of warifteine upon neutrophil adherence and migration in vitro demonstrated that the alkaloid decreased cell adhesion to P and E-selectin-transfected cells. In addition, fLMP-induced neutrophil migration in a transwell system was blocked by warifteine; this effect was mimicked by cAMP mimetic/inducing substances, and warifteine increased intracellular cAMP levels in neutrophils. The production of DNA extracellular traps (NETs) was also blocked by warifteine but there was no alteration on PMA-induced oxidative burst or LPS-stimulated TNF α secretion. Taken together, our data indicate that the alkaloid warifteine is a potent anti-inflammatory substance and that it has an effect on neutrophil migration through a decrease in both cell adhesion and migration.
authors.affiliation: Departamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2-062, Ilha do Fundão, 21944-570 Rio de Janeiro, RJ, Brazil.
authors.forename: Thaline F A
authors.initials: TF
authors.lastname: Lima
authors.affiliation: Departamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2-062, Ilha do Fundão, 21944-570 Rio de Janeiro, RJ, Brazil.
authors.forename: Juliana D B
authors.initials: JD
authors.lastname: Rocha
authors.affiliation: Departamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2-062, Ilha do Fundão, 21944-570 Rio de Janeiro, RJ, Brazil.
authors.forename: Anderson B
authors.initials: AB
authors.lastname: Guimarães-Costa
authors.affiliation: Departamento de Fisiologia e Patologia, Universidade Federal da Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: José M
authors.initials: JM
authors.lastname: Barbosa-Filho
authors.affiliation: Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, 23890-000 Seropédica, RJ, Brazil.
authors.forename: Débora
authors.initials: D
authors.lastname: Decoté-Ricardo
authors.affiliation: Departamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2-062, Ilha do Fundão, 21944-570 Rio de Janeiro, RJ, Brazil.
authors.forename: Elvira M
authors.initials: EM
authors.lastname: Saraiva
authors.affiliation: Departamento de Virologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, 21944-570 Rio de Janeiro, RJ, Brazil.
authors.forename: Luciana B
authors.initials: LB
authors.lastname: Arruda
authors.affiliation: Departamento de Fisiologia e Patologia, Universidade Federal da Paraíba, 58051-970 João Pessoa, PB, Brazil.
authors.forename: Marcia R
authors.initials: MR
authors.lastname: Piuvezam
authors.affiliation: Departamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2-062, Ilha do Fundão, 21944-570 Rio de Janeiro, RJ, Brazil.
authors.forename: Ligia M T
authors.initials: LM
authors.lastname: Peçanha
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects* / Q000276:immunology*; D031606:Cissampelos / Q000737:chemistry*; D003412:Cricetulus; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D042541:Intracellular Space / Q000378:metabolism; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C003931:warifteine; D000242:Cyclic AMP