title: Fabrication of single-hole glutathione-responsive degradable hollow silica nanoparticles for drug delivery.
abstract: In the present study, a kind of single-hole glutathione (GSH)-responsive degradable hollow silica nanoparticles (G-DHSNs) was synthesized and used as carriers of doxorubicin (DOX) (DOX-G-DHSNs). The G-DHSNs were accurately designed and fabricated with a simple and convenient method, and without any extra pernicious component. The composition, morphology and properties of the G-DHSNs had been characterized by (1)HNMR spectra, Fourier transform infrared spectrograph, thermo gravimetric analysis, transmission electron microscope, and scanning electron microscope. The degradation study of G-DHSNs showed that the G-DHSNs would be broken into pieces after interacting with GSH. Besides, the negligible hemolytic activity and low cytotoxicity of the G-DHSNs demonstrated its excellent biocompatibility. pH- and GSH-triggered release of DOX followed by the decomposition of G-DHSNs within TCA8113 cancer cells was further confirmed by flow cytometry and confocal laser scanning microscopy studies. All of these results indicated that G-DHSNs can be used as safe and promising drug nanocarriers.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry and ‡Institute of Physiology, School of Basic Medical Sciences, Lanzhou University , Lanzhou 730000, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Wang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xu
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Chen
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.forename: Cuilan
authors.initials: C
authors.lastname: Hou
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005434:Flow Cytometry; D005978:Glutathione / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011137:Polystyrenes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D013997:Time Factors
chemical_list: D011137:Polystyrenes; D012822:Silicon Dioxide; D004317:Doxorubicin; D005978:Glutathione