title: Tissue-specific proteome tagging: an orthogonal approach.
abstract: SORTing through the proteome: A versatile method has been developed for the incorporation of a novel 1,3-disubstituted cyclopropene amino acid into the proteome in response to a variety of sense codons and subsequent labeling. Incorporation can be confined to certain tissues by the selective expression of an orthogonal tRNA synthetase under cell-selective promoters.
authors.affiliation: Department of Chemistry, Carolina Center for Genome Sciences, The University of North Carolina at Chapel Hill, 125 South Road, Chapel Hill, NC 27599 (USA).
authors.forename: Adrienne L
authors.initials: AL
authors.lastname: Snyder
authors.forename: Eric M
authors.initials: EM
authors.lastname: Brustad
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D003062:Codon / Q000235:genetics; D006801:Humans; D009928:Organ Specificity / Q000235:genetics; D020543:Proteome / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D040901:Proteomics
chemical_list: D000596:Amino Acids; D003062:Codon; D020543:Proteome; D000604:Amino Acyl-tRNA Synthetases
keywords: bio-orthogonal chemistry; cycloaddition; genetic code expansion; proteomics; synthetases