title: Next-generation sequencing fragment library construction.
abstract: All current next-generation sequencing (NGS) platforms and applications require the sequencing library to have specific characteristics: in particular, size, size distribution, and 5' and 3' flanking sequences. This unit presents a robust protocol for converting a wide variety of input DNA samples into appropriate NGS libraries and discusses important considerations in experimental design, failure modes, and typical results.
authors.affiliation: Genomic Sequencing and Analysis Facility, University of Texas at Austin, Austin, Texas.
authors.forename: Jessica
authors.initials: J
authors.lastname: Podnar
authors.affiliation: Genomic Sequencing and Analysis Facility, University of Texas at Austin, Austin, Texas.
authors.forename: Heather
authors.initials: H
authors.lastname: Deiderick
authors.affiliation: Genomic Sequencing and Analysis Facility, University of Texas at Austin, Austin, Texas.
authors.forename: Scott
authors.initials: S
authors.lastname: Hunicke-Smith
mesh_terms: D000818:Animals; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*
keywords: DNA; NGS; amplicon; fragment; libraries; library; sequencing; shotgun