title: Duplex PCR for differentiation of the vaccine strain Brucella suis S2 and B. suis biovar 1 from other strains of Brucella spp.
abstract: Immunisation with attenuated Brucella spp. vaccines prevents brucellosis, but may also interfere with diagnosis. In this study, a duplex PCR was developed to distinguish Brucella suis vaccine strain S2 from field strains of B. suis biovar 1 and other Brucella spp. The PCR detected 60 fg genomic DNA of B. suis S2 or biovar 1 field strains and was able to distinguish B. suis S2 and wild-type strains of B. suis biovar 1 among 76 field isolates representing all the common species and biovars, as well as four vaccine strains, of Brucella.
authors.affiliation: Laboratory of Diagnostics Development, China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong 266032, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Nan
authors.affiliation: Laboratory of Diagnostics Development, China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong 266032, China; College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu 225009, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tan
authors.affiliation: Laboratory of Diagnostics Development, China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong 266032, China; College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu 225009, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Diagnostics Development, China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong 266032, China; College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu 225009, China.
authors.forename: Zouliang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: China Institute of Veterinary Drug Control, 8 Zhongguanchun South Street, Beijing 100081, China.
authors.forename: Kairong
authors.initials: K
authors.lastname: Mao
authors.affiliation: College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu 225009, China.
authors.forename: Daxin
authors.initials: D
authors.lastname: Peng
authors.affiliation: Laboratory of Diagnostics Development, China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong 266032, China. Electronic address: cyp777@163.com.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D002002:Brucella / Q000276:immunology; D002004:Brucella Vaccine / Q000276:immunology*; D041021:Brucella suis / Q000276:immunology*; D002006:Brucellosis / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012418:Ruminants; D013552:Swine; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D002004:Brucella Vaccine; D014613:Vaccines, Attenuated
keywords: Brucella suis biovar 1; Brucella suis vaccine strain S2; Diagnosis; PCR