title: [IFN-γ stimulation enhances immunosuppressive capability of human umbilical cord mesenchymal stem cells].
abstract: This study was objective to explore the effect of IFN-γ on immunosuppressive capability of mesenchymal stem cells (MSC) derived from umbilical cord. The immunomodulating capability of MSC was changed by stimulating cell surface receptors like Toll-like receptors (TLR). The inhibition of T-lymphocyte proliferation by MSC was tested via cell co-cultures. Further RT-PCR and ELISA were performed to examine the expression changes in gene and protein level. The results showed that the IFN-γ could promote the immunosuppressive effect of umbilical cord derived MSC. IFN-γ-stimulated MSC could suppress the proliferation of T cells more effectively. IFN-γ stimulation up-regulated the expression of immunosuppressive genes like IDO1, COX2, HLA-G, and soluble suppressive proteins such as HLA-G, KYN, IL10, PGE2 of MSC. And the immuno suppression capability of IFN-γ-stimulated MSC was 2-7 folds higher than control in MSC and lymphocyte co-culture tests. It is concluded that IFN-γ can effectively enhance the immunosuppressive capability of MSC.
authors.affiliation: Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China.
authors.forename: Xiao-Dun
authors.initials: XD
authors.lastname: Liu
authors.affiliation: Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China.
authors.forename: Chuan-Bao
authors.initials: CB
authors.lastname: Zang
authors.affiliation: Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ming
authors.affiliation: Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China.
authors.forename: Ling-Long
authors.initials: LL
authors.lastname: Chi
authors.affiliation: Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China.
authors.forename: Bai-Jun
authors.initials: BJ
authors.lastname: Shen
authors.affiliation: Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China; Cryomedical Laboratory, Shandong University Qilu Hospital, Jinan 250012, Shandong Province, China. E-mail:lidong73@sdu.edu.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D007165:Immunosuppression Therapy; D007371:Interferon-gamma / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D007371:Interferon-gamma