title: A strain-promoted alkyne-azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer-fluorescent conjugate for imaging of cancer cells.
abstract: For the first time, a novel EpCAM aptamer (SYL3C)-DIBO-AF594 fluorescent conjugate was synthesised by bioorthogonal chemistry utilizing a strain promoted alkyne-azide cycloaddition (copper free click) reaction (SPAAC). The ligation efficiency of SPAAC was improved by freeze-thaw cycles. The obtained conjugate showed target specific binding and aided in the imaging of various EpCAM positive cancer cell lines like MCF7, MDAMB453, Weri-RB1 and PC3.
authors.affiliation: Department of Nanobiotechnology, Kamalnayan Bajaj Research Institute, Vision Research Foundation, Chennai, India. drkrishnakumar_2000@yahoo.com.
authors.forename: Nithya
authors.initials: N
authors.lastname: Subramanian
authors.forename: Jagadeesh Babu
authors.initials: JB
authors.lastname: Sreemanthula
authors.forename: Baghavathi
authors.initials: B
authors.lastname: Balaji
authors.forename: Jagat R
authors.initials: JR
authors.lastname: Kanwar
authors.forename: Jyotirmay
authors.initials: J
authors.lastname: Biswas
authors.forename: Subramanian
authors.initials: S
authors.lastname: Krishnakumar
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D000951:Antigens, Neoplasm / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D001386:Azides / Q000737:chemistry*; D015815:Cell Adhesion Molecules / Q000032:analysis*; D045744:Cell Line, Tumor; D057930:Click Chemistry; D061565:Cycloaddition Reaction; D000071858:Epithelial Cell Adhesion Molecule; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis*; D061848:Optical Imaging
chemical_list: D000480:Alkynes; D000951:Antigens, Neoplasm; D052157:Aptamers, Nucleotide; D001386:Azides; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D005456:Fluorescent Dyes