title: A potent Brucella abortus 2308 Δery live vaccine allows for the differentiation between natural and vaccinated infection.
abstract: Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, P. R. China.
authors.forename: Junbo
authors.initials: J
authors.lastname: Zhang
authors.forename: Shuanghong
authors.initials: S
authors.lastname: Yin
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.forename: Ren
authors.initials: R
authors.lastname: Meng
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Huijun
authors.initials: H
authors.lastname: Shi
authors.forename: Shengwei
authors.initials: S
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Ni
authors.forename: Tiansen
authors.initials: T
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002004:Brucella Vaccine / Q000235:genetics / Q000276:immunology; D002003:Brucella abortus / Q000235:genetics* / Q000254:growth & development / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology* / Q000517:prevention & control*; D002460:Cell Line; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000096:biosynthesis / Q000276:immunology; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D002004:Brucella Vaccine; D007074:Immunoglobulin G; D014613:Vaccines, Attenuated; D015847:Interleukin-4; D007371:Interferon-gamma; D017853:Phosphotransferases (Alcohol Group Acceptor); C478753:erythritol kinase