title: CUPRAC-BCS and antioxidant activity assays as reliable markers of antioxidant capacity in erythrocytes.
abstract: OBJECTIVE
  The CUPRAC-BCS and antioxidant activity (AOA) assays have been developed as indirect methods to determine the total antioxidant capacity (TAC). We examined these assays as markers, in erythrocytes, under oxidative stress (induced by storage) and with antioxidants--vitamin C, L-carnitine, and curcumin.
  
  
  METHODS
  Antioxidants were added to blood samples, stored and assayed for TAC and reactive oxygen species (ROS).
  
  
  RESULTS
  Vitamin C at lower concentrations and L-carnitine and curcumin at higher concentrations proved beneficial to the erythrocytes.
  
  
  CONCLUSIONS
  CUPRAC-BCS and AOA effectively determined TAC of stored erythrocytes. This was in accordance with the levels of ROS, and hence, CUPRAC-BCS and AOA can be used as reliable markers of TAC, in erythrocytes during oxidative stress.
authors.forename: Ravikumar
authors.initials: R
authors.lastname: Soumya
authors.forename: Rajashekharaiah
authors.initials: R
authors.lastname: Vani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000378:metabolism / Q000494:pharmacology; D002331:Carnitine / Q000378:metabolism / Q000494:pharmacology; D003474:Curcumin / Q000378:metabolism / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D003474:Curcumin; D001205:Ascorbic Acid; D002331:Carnitine
keywords: Antioxidant activity; CUPRAC–BCS assay; DCFH-DA assay; Erythrocytes; Total antioxidant capacity