title: Evaluation of prognostic significance of circulating tumor cells detection in rectal cancer patients treated with preoperative radiotherapy: prospectively collected material data.
abstract: The aim of this study was to evaluate the prognostic value of circulating tumor cells (CTC) in nonmetastatic rectal cancer patients treated with short-term preoperative radiotherapy. In this single-center trial, 162 patients with rectal cancer after preoperative short-term radiotherapy (5 × 5 Gy) were recruited from January, 2008 to September, 2011. Clearance of CTC was determined in 91 patients enrolled in the molecular analysis. CTC presence was evaluated with real-time reverse transcription polymerase chain reaction assay (qPCR) based on the expression of three tumor genetic markers: carcinoembryonic antigen (CEA), cytokeratin 20 (CK20), and/or cancer stem cells marker CD133 (CEA/CK20/CD133). We found that CTC detection 7 days after surgery was of prognostic significance for the local recurrence (P value = 0.006). CTC detected preoperatively and 24 hours after resection had no prognostic value in cancer recurrence; however, there was a significant relationship between CTC prevalence 24 hours after surgery and lymph node metastasis (pN1-2). We also confirmed a significant clearance of CTC in peripheral blood (PB) 24 hours after surgery. Preoperative sampling is not significant for prognosis in rectal cancer patients treated with short-term radiotherapy. Detection of CTC in PB 7 days after surgery is an independent factor predicting local recurrence in this group of patients.
authors.affiliation: Department of Molecular and Translational Oncology, The Maria Skłodowska-Curie Cancer Center and Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland ; Department of Medical Genetics, Institute of Mother and Child, 01-211 Warsaw, Poland.
authors.forename: Dominika
authors.identifier: 0000-0002-7055-3458
authors.initials: D
authors.lastname: Nesteruk
authors.affiliation: Department of Oncological Gastroenterology, The Maria Skłodowska-Curie Cancer Center and Institute of Oncology, 02-781 Warsaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Rutkowski
authors.affiliation: Department of Surgery, John Paul Western Hospital, 05-825 Grodzisk Mazowiecki, Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Fabisiewicz
authors.affiliation: Department of Surgery, John Paul Western Hospital, 05-825 Grodzisk Mazowiecki, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Pawlak
authors.affiliation: Department of Molecular and Translational Oncology, The Maria Skłodowska-Curie Cancer Center and Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland.
authors.forename: Janusz A
authors.initials: JA
authors.lastname: Siedlecki
authors.affiliation: Department of Molecular and Translational Oncology, The Maria Skłodowska-Curie Cancer Center and Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland.
authors.forename: Anna
authors.identifier: 0000-0002-9334-1773
authors.initials: A
authors.lastname: Fabisiewicz
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008297:Male; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011300:Preoperative Care; D011379:Prognosis; D011446:Prospective Studies; D012004:Rectal Neoplasms / Q000097:blood / Q000473:pathology / Q000532:radiotherapy* / Q000601:surgery*