title: Vector competence of Aedes aegypti and Aedes vittatus (Diptera: Culicidae) from Senegal and Cape Verde archipelago for West African lineages of chikungunya virus.
abstract: To assess the risk of emergence of chikungunya virus (CHIKV) in West Africa, vector competence of wild-type, urban, and non-urban Aedes aegypti and Ae. vittatus from Senegal and Cape Verde for CHIKV was investigated. Mosquitoes were fed orally with CHIKV isolates from mosquitoes (ArD30237), bats (CS13-288), and humans (HD180738). After 5, 10, and 15 days of incubation following an infectious blood meal, presence of CHIKV RNA was determined in bodies, legs/wings, and saliva using real-time reverse transcription-polymerase chain reaction. Aedes vittatus showed high susceptibility (50-100%) and early dissemination and transmission of all CHIKV strains tested. Aedes aegypti exhibited infection rates ranging from 0% to 50%. Aedes aegypti from Cape Verde and Kedougou, but not those from Dakar, showed the potential to transmit CHIKV in saliva. Analysis of biology and competence showed relatively high infective survival rates for Ae. vittatus and Ae. aegypti from Cape Verde, suggesting their efficient vector capacity in West Africa.
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Cheikh T
authors.initials: CT
authors.lastname: Diagne
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Oumar
authors.initials: O
authors.lastname: Faye
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Guerbois
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Rachel
authors.initials: R
authors.lastname: Knight
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Diawo
authors.initials: D
authors.lastname: Diallo
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Ousmane
authors.initials: O
authors.lastname: Faye
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Yamar
authors.initials: Y
authors.lastname: Ba
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Ibrahima
authors.initials: I
authors.lastname: Dia
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Ousmane
authors.initials: O
authors.lastname: Faye
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Scott C
authors.initials: SC
authors.lastname: Weaver
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal.
authors.forename: Amadou A
authors.initials: AA
authors.lastname: Sall
authors.affiliation: Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal diallo@pasteur.sn.
authors.forename: Mawlouth
authors.initials: M
authors.lastname: Diallo
mesh_terms: D000330:Aedes / Q000821:virology*; D018354:Alphavirus Infections / Q000453:epidemiology / Q000635:transmission* / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D055726:Cabo Verde / Q000453:epidemiology; D002646:Chikungunya virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002685:Chiroptera / Q000821:virology; D005838:Genotype; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000821:virology; D012675:Senegal / Q000453:epidemiology; D016415:Sequence Alignment
chemical_list: D012367:RNA, Viral