title: Upregulation of intestinal glucose transporters after Roux-en-Y gastric bypass to prevent carbohydrate malabsorption.
abstract: OBJECTIVE
  To determine the effect of Roux-en-Y gastric bypass (RYGB) on the expression of intestinal sweet taste receptors (STRs), glucose transporters (GTs), glucose absorption, and glycemia.
  
  
  METHODS
  Intestinal biopsies were collected for mRNA expression of STR (T1R2) and GTs (SGLT-1 and GLUT2) from 11 non-diabetic RYGB, 13 non-diabetic obese, and 11 healthy subjects, at baseline and following a 30 min small intestinal (SI) glucose infusion (30 g/150 ml water with 3 g 3-O-methyl-d-glucopyranose (3-OMG)). Blood glucose, plasma 3-OMG, and insulin were measured for 270 min.
  
  
  RESULTS
  In RYGB patients, expression of both GTs was ∼2-fold higher at baseline and after glucose infusion than those of morbidly obese or healthy subjects (P < 0.001). STR expressions were comparable amongst the groups. Peak plasma 3-OMG in both RYGB (r = 0.69, P = 0.01) and obese (r = 0.72, P = 0.005) correlated with baseline expression of SGLT-1, as was the case with peak blood glucose in RYGB subjects (r = 0.69, P = 0.02).
  
  
  CONCLUSIONS
  The upregulated intestinal GTs in RYGB patients are associated with increased glucose absorption when glucose is delivered at a physiological rate, suggesting a molecular adaptation to prevent carbohydrate malabsorption from rapid intestinal transit after RYGB.
authors.affiliation: Department of Gastroenterology and Hepatology, Royal Adelaide Hospital, Adelaide, South Australia, 5000, Australia; Discipline of Medicine, University of Adelaide, Royal Adelaide Hospital, Adelaide, South Australia, 5000, Australia.
authors.forename: Nam Q
authors.initials: NQ
authors.lastname: Nguyen
authors.forename: Tamara L
authors.initials: TL
authors.lastname: Debreceni
authors.forename: Jenna E
authors.initials: JE
authors.lastname: Bambrick
authors.forename: Bridgette
authors.initials: B
authors.lastname: Chia
authors.forename: Adam M
authors.initials: AM
authors.lastname: Deane
authors.forename: Gary
authors.initials: G
authors.lastname: Wittert
authors.forename: Chris K
authors.initials: CK
authors.lastname: Rayner
authors.forename: Michael
authors.initials: M
authors.lastname: Horowitz
authors.forename: Richard L
authors.initials: RL
authors.lastname: Young
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D050260:Carbohydrate Metabolism; D016022:Case-Control Studies; D005260:Female; D015390:Gastric Bypass / Q000009:adverse effects; D005947:Glucose / Q000493:pharmacokinetics; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000097:blood; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D008286:Malabsorption Syndromes / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000235:genetics / Q000378:metabolism / Q000601:surgery*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D001786:Blood Glucose; D051246:Glucose Transport Proteins, Facilitative; D007328:Insulin; D005947:Glucose