title: Characteristics of γδ T cells in Schistosoma japonicum-infected mouse mesenteric lymph nodes.
abstract: Gamma delta (γδ) T cells are mainly present in mucosa-associated lymphoid tissues, which play an important role in mucosal immunity. In this study, C57BL/6 mice were infected by Schistosoma japonicum and lymphocytes were isolated from the mesenteric lymph node (MLN) to identify changes in the phenotype and function of γδ T cells using flow cytometry. Our results indicated that the absolute number of γδ T cells from the MLNs of infected mice was significantly higher compared with normal mice (P < 0.05). In addition, the infected γδ T cells expressed a high level of the activated molecule CD69 (P < 0.01) and demonstrated an increasing population of CD4(+) γδ T cells (P < 0.05). MLN γδ T cells secrete interferon-γ (IFN-γ), interleukin (IL)-4, IL-9, and IL-17 in response to propylene glycol monomethyl acetate (PMA) plus ionomycin simulation, and the levels of IL-4, IL-9, and IL-17 increased significantly after S. japonicum infection (P < 0.05). Taken together, these findings indicated that S. japonicum infection could induce γδ T cell activation, proliferation, and differentiation in the MLN. Moreover, our results indicated that the expression of NKG2D (CD314) was not increased in γδ T cells after infection, suggesting that other mechanisms are involved in activating γδ T cells. Furthermore, higher expression of programmed death-1 (CD279) but not IL-10 was detected in the γδ T cells isolated from infected mice (P < 0.05), suggesting that the function of γδ T cells is inhibited gradually over the course of S. japonicum infection.
authors.affiliation: Department of Pathogenic Biology and Immunology, Guangzhou Medical University, 510182, Guangzhou, China, 894398906@qq.com.
authors.forename: Xiuxue
authors.initials: X
authors.lastname: Yu
authors.forename: Xueping
authors.initials: X
authors.lastname: Luo
authors.forename: Hongyan
authors.initials: H
authors.lastname: Xie
authors.forename: Dianhui
authors.initials: D
authors.lastname: Chen
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.forename: Changyou
authors.initials: C
authors.lastname: Wu
authors.forename: Anping
authors.initials: A
authors.lastname: Peng
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology* / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology*; D008643:Mesentery / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D012549:Schistosoma japonicum; D012554:Schistosomiasis japonica / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000145:classification* / Q000378:metabolism
chemical_list: D007378:Interleukins; D007371:Interferon-gamma