title: Susceptibility of opportunistic Burkholderia glumae to copper surfaces following wet or dry surface contact.
abstract: Burkholderia glumae has been proposed to have a potential risk to vulnerable communities. In this work, we investigated the antibacterial activity and mechanism of copper surfaces against multi-drug resistant B. glumae from both patients and rice plants. The susceptibility of B. glumae to copper surfaces was noted by a significant decline in viable bacterial counts, relative to the slight reduction of stainless steel and polyvinylchloride, both of which were used as control surfaces. The mode of action of bacterial killing was determined by examing the mutagenicity, DNA damage, copper ions accumulation, and membrane damage in bacterial cells. The results indicated that the cells exposed to copper surfaces did not cause severe DNA lesions or increase the mutation frequencies, but resulted in a loss of cell membrane integrity within minutes. Furthermore, bacterial cells exposed to copper surfaces accumulated significantly higher amounts of copper compared to control surfaces. Overall, this study showed that metallic copper had strong antibacterial effect against B. glumae by causing DNA and membrane damage, cellular accumulation of copper, and cell death following DNA degradation, which could be utilized to reduce the risk of bacterial contamination and infection.
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. twelue@163.com.
authors.forename: Zhouqi
authors.initials: Z
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. ibrahim@ciitsahiwal.edu.pk.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ibrahim
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. clyang0202@163.com.
authors.forename: Chunlan
authors.initials: C
authors.lastname: Yang
authors.affiliation: College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China. fy0579@zjnu.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology, Sahiwal 57000, Pakistan. anam.hussain63@yahoo.com.
authors.forename: Hussain
authors.initials: H
authors.lastname: Annam
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. ylwang88@aliyun.com.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. glxie@zju.edu.cn.
authors.forename: Guan-Lin
authors.initials: GL
authors.lastname: Xie
authors.affiliation: Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. sungc01@sina.com.
authors.forename: Guochang
authors.initials: G
authors.lastname: Sun
mesh_terms: D000900:Anti-Bacterial Agents; D001422:Bacterial Adhesion; D019117:Burkholderia / Q000235:genetics; D002462:Cell Membrane; D003300:Copper; D004249:DNA Damage; D050296:Microbial Viability; D009152:Mutagenicity Tests; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D003300:Copper