title: Enzymatic production of 5-aminovalerate from L-lysine using L-lysine monooxygenase and 5-aminovaleramide amidohydrolase.
abstract: 5-Aminovalerate is a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. It is a metabolite of l-lysine catabolism through the aminovalerate pathway in Pseudomonas putida. L-Lysine monooxygenase (DavB) and 5-aminovaleramide amidohydrolase (DavA) play key roles in the biotransformation of L-lysine into 5-aminovalerate. Here, DavB and DavA of P. putida KT2440 were expressed, purified, and coupled for the production of 5-aminovalerate from L-lysine. Under optimal conditions, 20.8 g/L 5-aminovalerate was produced from 30 g/L L-lysine in 12 h. Because L-lysine is an industrial fermentation product, the two-enzyme coupled system presents a promising alternative for the production of 5-aminovalerate.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China.
authors.forename: Pan
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China.
authors.forename: Mandong
authors.initials: M
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.affiliation: 1] State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China [2].
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China.
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D021542:Amino Acids, Neutral / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology; D008239:Lysine / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D016958:Pseudomonas putida / Q000378:metabolism
chemical_list: D021542:Amino Acids, Neutral; C013809:5-aminovaleric acid; D006899:Mixed Function Oxygenases; C100031:lysine monooxygenase; D000581:Amidohydrolases; D008239:Lysine