title: MicroRNA-218 inhibits cell invasion and migration of pancreatic cancer via regulating ROBO1.
abstract: miRNA-218 is a highlighted tumor suppressor and its underlying role in tumor progression is still unknown. Here, we restored the expression of miRNA-218 in pancreatic cancer to clarify the function and potent downstream pathway of miRNA-218. The expressions of both miRNA-218 and its potent target gene ROBO1 were revealed by RT-PCR and western blotting analysis. Transfection of miRNA-218 precursor mimics and luciferase assay were performed to elucidate the regulation mechanism between miRNA-218 and ROBO1. Cells, stably expressing miRNA-218 followed by forced expression of mutant ROBO1, were established through co-transfections of both lentivirus vector and plasmid vector. The cell migration and invasion abilities were evaluated by migration assay and invasion assay respectively. An increased expression of ROBO1 was revealed in cell BxPC-3-LN compared with cell BxPC-3. Elevated expression of miRNA-218 would suppress the expression of ROBO1 via complementary binding to a specific region within 3'UTR of ROBO1 mRNA (sites 971-978) in pancreatic cancer cells. Stably restoring the expression of miRNA-218 in pancreatic cancer significantly downregulated the expression of ROBO1 and effectively inhibited cell migration and invasion. Forced expression of mutant ROBO1 could reverse the repression effects of miRNA-218 on cell migration and invasion. Consequently, miRNA-218 acted as a tumor suppressor in pancreatic cancer by inhibiting cell invasion and migration. ROBO1 was a functional target of miRNA-218's downstream pathway involving in cell invasion and migration of pancreatic cancer.
authors.affiliation: Pancreatic Disease Institute; Department of Pancreatic Surgery; Huashan Hospital; Shanghai Medical College; Fudan University; Shanghai, PR China.
authors.forename: Hang
authors.initials: H
authors.lastname: He
authors.affiliation: Pancreatic Disease Institute; Department of Pancreatic Surgery; Huashan Hospital; Shanghai Medical College; Fudan University; Shanghai, PR China.
authors.forename: Si-Jie
authors.initials: SJ
authors.lastname: Hao
authors.affiliation: Pancreatic Disease Institute; Department of Pancreatic Surgery; Huashan Hospital; Shanghai Medical College; Fudan University; Shanghai, PR China.
authors.forename: Lie
authors.initials: L
authors.lastname: Yao
authors.affiliation: Pancreatic Disease Institute; Department of Pancreatic Surgery; Huashan Hospital; Shanghai Medical College; Fudan University; Shanghai, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.affiliation: Pancreatic Disease Institute; Department of Pancreatic Surgery; Huashan Hospital; Shanghai Medical College; Fudan University; Shanghai, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Di
authors.affiliation: Pancreatic Disease Institute; Department of Pancreatic Surgery; Huashan Hospital; Shanghai Medical College; Fudan University; Shanghai, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Pancreatic Disease Institute; Department of Pancreatic Surgery; Huashan Hospital; Shanghai Medical College; Fudan University; Shanghai, PR China.
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Jiang
authors.affiliation: Pancreatic Disease Institute; Department of Pancreatic Surgery; Huashan Hospital; Shanghai Medical College; Fudan University; Shanghai, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Jin
authors.affiliation: Pancreatic Disease Institute; Department of Pancreatic Surgery; Huashan Hospital; Shanghai Medical College; Fudan University; Shanghai, PR China.
authors.forename: De-Liang
authors.initials: DL
authors.lastname: Fu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009361:Neoplasm Invasiveness / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D000097905:Roundabout Proteins
chemical_list: D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; C547471:MIRN218 microRNA, human
keywords: ROBO1; invasion; microRNA-218; migration; pancreatic cancer