title: Soybean Bowman-Birk protease inhibitor (BBI): identification of the mechanisms of BBI suppressive effect on growth of two adenocarcinoma cell lines: AGS and HT29.
abstract: OBJECTIVE
  Bowman-Birk protease inhibitor (BBI) has been well known to suppress the emergence and progression of different cancers. In the present study, the mechanisms by which BBI alters cancers have been addressed. To reach this goal, the effects of BBI on proliferation of and VEGF secretion by two cell lines (AGS: gastric adenocarcinoma and HT-29: colorectal adenocarcinoma) and also BBI effect on MMP-2 and 9 synthesis/secretion by AGS cells was evaluated.
  
  
  METHODS
  ELISA method was used to assess VEGF concentration and gelatin zymography was used to address MMP-2 and 9 production/excretion.
  
  
  RESULTS
  BBI had powerful inhibitory effect on proliferation and VEGF secretion by both cell lines. In addition, inhibition of MMP-2 and MMP-9 secreted by AGS cells suggests BBI as a potent inhibitor of gastric cancer progression. On the other hand, the results indicated that inhibition of MMP-2, MMP-9 and VEGF secretion is one of the mechanisms of anti-angiogenic effect of BBI.
  
  
  CONCLUSIONS
  BBI expresses powerful suppressive effect on tumor progression of two prevalent cancers: gastric adenocarcinoma and colorectal adenocarcinoma.
authors.affiliation: Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Amirhossein
authors.initials: A
authors.lastname: Fereidunian
authors.affiliation: Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Sadeghalvad
authors.affiliation: Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Maryam Omidi
authors.initials: MO
authors.lastname: Oscoie
authors.affiliation: Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran; Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic address: amostafaie@kums.ac.ir.
authors.forename: Ali
authors.initials: A
authors.lastname: Mostafaie
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D019073:HT29 Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000494:pharmacology* / Q000627:therapeutic use; D014361:Trypsin Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D014361:Trypsin Inhibitors; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: Angiogenesis; Bowman-Birk protease inhibitor; Matrix metalloproteinase; Purification; Vascular endothelial growth factor