title: Improved method for drawing of a glycan map, and the first page of glycan atlas, which is a compilation of glycan maps for a whole organism.
abstract: Glycan Atlas is a set of glycan maps over the whole body of an organism. The glycan map that includes data of glycan structure and quantity displays micro-heterogeneity of the glycans in a tissue, an organ, or cells. The two-dimensional glycan mapping is widely used for structure analysis of N-linked oligosaccharides on glycoproteins. In this study we developed a comprehensive method for the mapping of both N- and O-glycans with and without sialic acid. The mapping data of 150 standard pyridylaminated glycans were collected. The empirical additivity rule which was proposed in former reports was able to adapt for this extended glycan map. The adapted rule is that the elution time of pyridylamino glycans on high performance liquid chromatography (HPLC) is expected to be the simple sum of the partial elution times assigned to each monosaccharide residue. The comprehensive mapping method developed in this study is a powerful tool for describing the micro-heterogeneity of the glycans. Furthermore, we prepared 42 pyridylamino (PA-) glycans from human serum and were able to draw the map of human serum N- and O-glycans as an initial step of Glycan Atlas editing.
authors.affiliation: Department of Biology, Faculty of Science, Niigata University, Niigata, Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Natsuka
authors.affiliation: Department of Biology, Faculty of Science, Niigata University, Niigata, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Masuda
authors.affiliation: Life Science Research Center, Kagawa University, Takamatsu, Kagawa, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Sumiyoshi
authors.affiliation: Life Science Research Center, Kagawa University, Takamatsu, Kagawa, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nakakita
mesh_terms: D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D011134:Polysaccharides / Q000097:blood / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; D019158:N-Acetylneuraminic Acid