title: Der p 11 is a major allergen for house dust mite-allergic patients suffering from atopic dermatitis.
abstract: House dust mites (HDMs) belong to the most potent indoor allergen sources worldwide and are associated with allergic manifestations in the respiratory tract and the skin. Here we studied the importance of the high-molecular-weight group 11 allergen from Dermatophagoides pteronyssinus (Der p 11) in HDM allergy. Sequence analysis showed that Der p 11 has high homology to paramyosins from mites, ticks, and other invertebrates. A synthetic gene coding for Der p 11 was expressed in Escherichia coli and rDer p 11 purified to homogeneity as folded, alpha-helical protein as determined by circular dichroism spectroscopy. Using antibodies raised against rDer p 11 and immunogold electron microscopy, the allergen was localized in the muscle beneath the skin of mite bodies but not in feces. IgE reactivity of rDer p 11 was tested with sera from HDM-allergic patients from Europe and Africa in radioallergosorbent test-based dot-blot assays. Interestingly, we found that Der p 11 is a major allergen for patients suffering from atopic dermatitis (AD), whereas it is only a minor allergen for patients suffering from respiratory forms of HDM allergy. Thus, rDer p 11 might be a useful serological marker allergen for the identification of a subgroup of HDM-allergic patients suffering from HDM-associated AD.
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Srinita
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Resch
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Kuan-Wei
authors.initials: KW
authors.lastname: Chen
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria; Christian Doppler Laboratory for Allergy Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Ines
authors.initials: I
authors.lastname: Swoboda
authors.affiliation: Christian Doppler Laboratory for Allergy Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Margit
authors.initials: M
authors.lastname: Focke-Tejkl
authors.affiliation: Division of Hematology and Hemostaseology, Department of Internal Medicine I, Medical University of Vienna, Vienna, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Blatt
authors.affiliation: Department of Dermatology and Allergy, University of Bonn, Bonn, Germany.
authors.forename: Natalija
authors.initials: N
authors.lastname: Novak
authors.affiliation: Sachs' Children's Hospital and Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Magnus
authors.initials: M
authors.lastname: Wickman
authors.affiliation: Clinical Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet and University Hospital Stockholm, Stockholm, Sweden.
authors.forename: Marianne
authors.initials: M
authors.lastname: van Hage
authors.affiliation: Center for Molecular Allergology, IDI-IRCCS, Rome, Italy.
authors.forename: Rosetta
authors.initials: R
authors.lastname: Ferrara
authors.affiliation: Center for Molecular Allergology, IDI-IRCCS, Rome, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Mari
authors.affiliation: Service de Pneumologie, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.
authors.forename: Ashok
authors.initials: A
authors.lastname: Purohit
authors.affiliation: Service de Pneumologie, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Pauli
authors.affiliation: Gamma City Laboratory, Harare, Zimbabwe.
authors.forename: Elopy N
authors.initials: EN
authors.lastname: Sibanda
authors.affiliation: Gamma City Laboratory, Harare, Zimbabwe.
authors.forename: Portia
authors.initials: P
authors.lastname: Ndlovu
authors.affiliation: Center for Child Health Research, Telethon Institute of Child Health Research, University of Western Australia, Perth, Australia.
authors.forename: Wayne R
authors.initials: WR
authors.lastname: Thomas
authors.affiliation: Institute of Scientific Instruments, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
authors.forename: Vladislav
authors.initials: V
authors.lastname: Krzyzanek
authors.affiliation: Institute of Medical Physics and Biophysics, University of Münster, Münster, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Tacke
authors.affiliation: Institute of Medical Physics and Biophysics, University of Münster, Münster, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Malkus
authors.affiliation: Division of Hematology and Hemostaseology, Department of Internal Medicine I, Medical University of Vienna, Vienna, Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Valent
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria; Christian Doppler Laboratory for Allergy Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Valenta
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria; Christian Doppler Laboratory for the Development of Allergen Chips, Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria. Electronic address: susanne.vrtala@meduniwien.ac.at.
authors.forename: Susanne
authors.initials: S
authors.lastname: Vrtala
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D039741:Antigens, Dermatophagoides / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D060829:Arthropod Proteins; D015415:Biomarkers; D002648:Child; D002942:Circular Dichroism; D003876:Dermatitis, Atopic / Q000453:epidemiology / Q000276:immunology*; D040002:Dermatophagoides pteronyssinus / Q000235:genetics* / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D016036:Seroepidemiologic Studies; D014335:Tropomyosin / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D055815:Young Adult
chemical_list: D000906:Antibodies; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D015415:Biomarkers; C000595545:Der p 11 allergen, Dermatophagoides pteronyssinus; D014335:Tropomyosin; D007073:Immunoglobulin E