title: Different uranium distribution patterns in cytosolic protein pool of zebrafish gills after chronic and acute waterborne exposures.
abstract: The toxicity of uranium (U) to aquatic organisms depends notably on its compartmentalization in organs, tissues, cells as well as on its distribution among biomolecules. In order to contribute to the understanding of U accumulation and associated toxicity mechanisms in case of waterborne exposure, this study focused on U fate in the gills epithelia, uptake pathway, of the fish model Danio rerio (zebrafish). U distribution among cytosolic biomolecules was investigated after no addition (0μgL(-)(1) (c0) for 3 and 30d), chronic (20μgL(-)(1) (c20) for 30d) and acute (20μgL(-)(1) (c20) and 250μgL(-)(1) (c250) for 3d) exposures to depleted U. Cytosolic U accounted for an average of 24-32% of gills burden for c20 and c250, respectively. Size Exclusion Chromatography (SEC) coupled with Inductively Coupled Plasma-Sector Field Mass Spectrometry (ICP-SFMS) allowed identification of ecotoxicologically relevant U-containing fractions among cytosolic biomolecules as a function of exposure conditions. In c0 and c20 samples, most U (ca.80%) was found in the Low Molecular Weight fraction (LMW, <18kDa), often considered as a detoxifying fraction. In c250 exposed fish, U was equally distributed between LMW (40%) and High Molecular Weight (HMW, 150-670kDa; 40%) fractions, the latter including sensitive metalloproteins. Uranium-biomolecules were co-eluted with endogenous essential metal (Fe, Cu and Zn) species, however, no major influence on their cytosolic concentration and distribution pattern among cytosolic proteins was found.
authors.affiliation: IRSN/PRP-ENV/SERIS, Laboratoire de Biogéochimie, Biodisponibilité et Transferts des radionucléides, BP3, 13115 St Paul lez Durance Cedex, France; LCABIE, UMR5254, Technopôle Hélioparc Pau Pyrénées, 2 avenue du Président Angot, 64053 Pau Cedex 09, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Bucher
authors.affiliation: LCABIE, UMR5254, Technopôle Hélioparc Pau Pyrénées, 2 avenue du Président Angot, 64053 Pau Cedex 09, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Mounicou
authors.affiliation: IRSN/PRP-ENV/SERIS, Laboratoire de Biogéochimie, Biodisponibilité et Transferts des radionucléides, BP3, 13115 St Paul lez Durance Cedex, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Simon
authors.affiliation: IRSN/PRP-ENV/SERIS, Laboratoire de Biogéochimie, Biodisponibilité et Transferts des radionucléides, BP3, 13115 St Paul lez Durance Cedex, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Floriani
authors.affiliation: LCABIE, UMR5254, Technopôle Hélioparc Pau Pyrénées, 2 avenue du Président Angot, 64053 Pau Cedex 09, France.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Lobinski
authors.affiliation: IRSN/PRP-ENV/SERIS, Laboratoire de Biogéochimie, Biodisponibilité et Transferts des radionucléides, BP3, 13115 St Paul lez Durance Cedex, France. Electronic address: sandrine.frelon@irsn.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Frelon
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D003600:Cytosol / Q000737:chemistry* / Q000378:metabolism; D005880:Gills / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D008670:Metals / Q000032:analysis; D011506:Proteins / Q000737:chemistry*; D014501:Uranium / Q000032:analysis*; D014875:Water Pollutants, Radioactive / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D008670:Metals; D011506:Proteins; D014875:Water Pollutants, Radioactive; D014501:Uranium
keywords: Cytosolic distribution; Gills; SEC–ICP-SFMS; Uranium speciation; Zebrafish