title: Pyrophosphate selective fluorescent chemosensors: cascade recognition of nuclear stain mimicking DAPI.
abstract: A new zinc(ii) complex with a condensed hydroxynaphthyl pyridine (SPHN) as the coordinated ligand has been synthesized for the selective recognition of pyrophosphate (PPi) over other anions including phosphate in a mixed aqueous solution. The fluorescence enhancement of SPHN in association with Zn(2+) ions is quenched in the presence of intracellular pyrophosphate. This phenomenon is utilized in the construction of a logic gate. The binding of SPHN with Zn(2+) and its displacement by PPi have been established by photophysical investigation and supported by the DFT level of studies. The development of blue fluorescence in the {} complex upon binding of zinc with is shown to be useful as a nucleus marker in a cell similar to the commercially available staining compound, DAPI (diamino-2-phenylindole).
authors.affiliation: Department of Chemistry, Indian Institute of Engineering Science and Technology, Shibpur, Howrah-711 103, India. spgoswamical@yahoo.com.
authors.forename: Shyamaprosad
authors.initials: S
authors.lastname: Goswami
authors.forename: Avijit Kumar
authors.initials: AK
authors.lastname: Das
authors.forename: Bholanath
authors.initials: B
authors.lastname: Pakhira
authors.forename: Sohini
authors.initials: S
authors.lastname: Basu Roy
authors.forename: Anup Kumar
authors.initials: AK
authors.lastname: Maity
authors.forename: Partha
authors.initials: P
authors.lastname: Saha
authors.forename: Sabyasachi
authors.initials: S
authors.lastname: Sarkar
mesh_terms: D011756:Diphosphates / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D011756:Diphosphates; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; C107241:diphosphoric acid