title: Characterization, antioxidant and antitumor activities of polysaccharides from Salvia miltiorrhiza Bunge.
abstract: Polysaccharides were extracted from Salvia miltiorrhiza Bunge using response surface methodology (RSM) with ultrasonication. A Box-Behnken design was used to optimize the extraction parameters to maximize the polysaccharide extraction yield. The polysaccharide SMP-U1 was isolated and characterized; then the antioxidant and antiproliferation activities were evaluated in vitro. The modified optimal conditions were an ultrasonic power of 180 W, an extraction temperature of 54°C, and an extraction time of 32 min, achieving an extraction yield of 40.54±0.25%. The results indicate that SMP-U1 has significant antioxidant activity, scavenging the free radical 2,2-diphenyl-1-picrylhydrazyl. It has also exhibited effect on the proliferation of human breast carcinoma cells Bcap-37 and human esophageal carcinoma cells Eca-109, especially at a concentration of 0.30 mg/mL. In conclusion, SMP-U1 has remarkable in vitro antioxidant and antiproliferation activity, and has potential for application as a natural antioxidant or antitumor agent.
authors.affiliation: College of Life Science, Sichuan Agricultural University, Yaan 625014, Sichuan, PR China.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Jiang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Basic Science, Sichuan Agricultural University, Yaan 625014, Sichuan, PR China. Electronic address: zhang8434@sina.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Life Science, Sichuan Agricultural University, Yaan 625014, Sichuan, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Chengdu Dadi Hank Bio-technology Company, Wenjiang 611130, Sichuan, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Yaan 625014, Sichuan, PR China.
authors.forename: Chun-bang
authors.initials: CB
authors.lastname: Ding
authors.affiliation: College of Life Science, Sichuan Agricultural University, Yaan 625014, Sichuan, PR China.
authors.forename: Rui-wu
authors.initials: RW
authors.lastname: Yang
authors.affiliation: College of Life Science, Sichuan Agricultural University, Yaan 625014, Sichuan, PR China.
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Wang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, PR China.
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Zhou
mesh_terms: D000704:Analysis of Variance; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002241:Carbohydrates / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014574:Uronic Acids / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D002241:Carbohydrates; D016166:Free Radical Scavengers; D010936:Plant Extracts; D010940:Plant Proteins; D011134:Polysaccharides; D014574:Uronic Acids
keywords: Bioactivity; Polysaccharides; Salvia miltiorrhiza Bunge