title: Disruption of Mbd5 in mice causes neuronal functional deficits and neurobehavioral abnormalities consistent with 2q23.1 microdeletion syndrome.
abstract: 2q23.1 microdeletion syndrome is characterized by intellectual disability, motor delay, autistic-like behaviors, and a distinctive craniofacial phenotype. All patients carry a partial or total deletion of methyl-CpG-binding domain protein 5 (MBD5), suggesting that haploinsufficiency of this gene is responsible for the phenotype. To confirm this hypothesis and to examine the role of MBD5 in vivo, we have generated and characterized an Mbd5 gene-trap mouse model. Our study indicates that the Mbd5(+/) (GT) mouse model recapitulates most of the hallmark phenotypes observed in 2q23.1 deletion carriers including abnormal social behavior, cognitive impairment, and motor and craniofacial abnormalities. In addition, neuronal cultures uncovered a deficiency in neurite outgrowth. These findings support a causal role of MBD5 in 2q23.1 microdeletion syndrome and suggest a role for MBD5 in neuronal processes. The Mbd5(+/) (GT) mouse model will advance our understanding of the abnormal brain development underlying the emergence of 2q23.1 deletion-associated behavioral and cognitive symptoms.
authors.affiliation: Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami, Miami, FL, USA.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Camarena
authors.affiliation: Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami, Miami, FL, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.affiliation: John P. Hussman Institute for Human Genomics, Miller School of Medicine University of Miami, Miami, FL, USA.
authors.forename: Clemer
authors.initials: C
authors.lastname: Abad
authors.affiliation: Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami, Miami, FL, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Abrams
authors.affiliation: Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami, Miami, FL, USA.
authors.forename: Yaima
authors.initials: Y
authors.lastname: Toledo
authors.affiliation: Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan.
authors.forename: Kimi
authors.initials: K
authors.lastname: Araki
authors.affiliation: Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan.
authors.forename: Masatake
authors.initials: M
authors.lastname: Araki
authors.affiliation: Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami, Miami, FL, USA John P. Hussman Institute for Human Genomics, Miller School of Medicine University of Miami, Miami, FL, USA.
authors.forename: Katherina
authors.initials: K
authors.lastname: Walz
authors.affiliation: Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami, Miami, FL, USA John P. Hussman Institute for Human Genomics, Miller School of Medicine University of Miami, Miami, FL, USA jyoung3@med.miami.edu.
authors.forename: Juan I
authors.initials: JI
authors.lastname: Young
mesh_terms: D000818:Animals; D002872:Chromosome Deletion; D033481:Chromosomes, Mammalian; D017353:Gene Deletion; D051783:Methyl-CpG-Binding Protein 2 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009422:Nervous System Diseases / Q000235:genetics*
chemical_list: C497393:Mecp2 protein, mouse; D051783:Methyl-CpG-Binding Protein 2
keywords: MBD5; autistic disorder; intellectual disability; mouse model