title: Overexpressed ubiquitin ligase Cullin7 in breast cancer promotes cell proliferation and invasion via down-regulating p53.
abstract: Ubiquitin ligase Cullin7 has been identified as an oncogene in some malignant diseases such as choriocarcinoma and neuroblastoma. However, the role of Cullin7 in breast cancer carcinogenesis remains unclear. In this study, we compared Cullin7 protein levels in breast cancer tissues with normal breast tissues and identified significantly higher expression of Cullin7 protein in breast cancer specimens. By overexpressing Cullin7 in breast cancer cells HCC1937, we found that Cullin7 could promote cell growth and invasion in vitro. In contrast, the cell growth and invasion was inhibited by silencing Cullin7 in breast cancer cell BT474. Moreover, we demonstrated that Cullin7 promoted breast cancer cell proliferation and invasion via down-regulating p53 expression. Thus, our study provided evidence that Cullin7 functions as a novel oncogene in breast cancer and may be a potential therapeutic target for breast cancer management.
authors.affiliation: Department of Histology and Embryology, Guangdong Medical College, Dongguan 523808, Guangdong, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Guo
authors.affiliation: The 7th People's Hospital of Chengdu, Chengdu 610041, Sichuan, China.
authors.forename: Fenping
authors.initials: F
authors.lastname: Wu
authors.affiliation: The Second School of Clinical Medicine, Guangdong Medical College, Dongguan 523808, Guangdong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Guangdong Medical College, Dongguan 523808, Guangdong, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Yan
authors.affiliation: Oncology of Affiliated Hospital Guangdong Medical College, Zhanjiang 524000, Guangdong, China. Electronic address: wenmeisutg@126.com.
authors.forename: Wenmei
authors.initials: W
authors.lastname: Su
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D044842:Cullin Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D005260:Female; D006801:Humans; D009361:Neoplasm Invasiveness; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C506299:CUL7 protein, human; D044842:Cullin Proteins; D017931:DNA Primers; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: Breast cancer; Cullin7; Invasion; Proliferation; p53