title: Sensitive flow-injection spectrophotometric analysis of bromopride.
abstract: A flow injection spectrophotometric procedure employing merging zones is proposed for direct bromopride determination in pharmaceutical formulations and biological fluids. The proposed method is based on the reaction between bromopride and p-dimethylaminocinnamaldehyde (p-DAC) in acid medium, in the presence of sodium dodecyl sulfate (SDS), resulting in formation of a violet product (λmax=565nm). Experimental design methodologies were used to optimize the experimental conditions. The Beer-Lambert law was obeyed in a bromopride concentration range of 3.63×10(-7) to 2.90×10(-5)molL(-1), with a correlation coefficient (r) of 0.9999. The limits of detection and quantification were 1.07×10(-7) and 3.57×10(-7)molL(-1), respectively. The proposed method was successfully applied to the determination of bromopride in pharmaceuticals and human urine, and recoveries of the drug from these media were in the ranges 99.6-101.2% and 98.6-102.1%, respectively. This new flow injection procedure does not require any sample pretreatment steps.
authors.affiliation: Instituto de Química, Universidade Estadual Paulista "Julio de Mesquita Filho", UNESP, R. Prof. Francisco Degni 55, P.O. Box 355, 14800-900 Araraquara, SP, Brazil.
authors.forename: Liliane Spazzapam
authors.initials: LS
authors.lastname: Lima
authors.affiliation: Instituto de Química, Universidade Estadual Paulista "Julio de Mesquita Filho", UNESP, R. Prof. Francisco Degni 55, P.O. Box 355, 14800-900 Araraquara, SP, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Los Weinert
authors.affiliation: Instituto de Química, Universidade Estadual Paulista "Julio de Mesquita Filho", UNESP, R. Prof. Francisco Degni 55, P.O. Box 355, 14800-900 Araraquara, SP, Brazil.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Pezza
authors.affiliation: Instituto de Química, Universidade Estadual Paulista "Julio de Mesquita Filho", UNESP, R. Prof. Francisco Degni 55, P.O. Box 355, 14800-900 Araraquara, SP, Brazil. Electronic address: hrpezza@iq.unesp.br.
authors.forename: Helena Redigolo
authors.initials: HR
authors.lastname: Pezza
mesh_terms: D002934:Cinnamates / Q000737:chemistry; D018492:Dopamine Antagonists / Q000032:analysis / Q000652:urine*; D016903:Drug Monitoring / Q000295:instrumentation; D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation*; D006801:Humans; D057230:Limit of Detection; D008787:Metoclopramide / Q000031:analogs & derivatives* / Q000032:analysis / Q000652:urine; D004364:Pharmaceutical Preparations / Q000737:chemistry; D013053:Spectrophotometry / Q000295:instrumentation*
chemical_list: D002934:Cinnamates; D018492:Dopamine Antagonists; D004364:Pharmaceutical Preparations; C013855:bromopride; C018523:4-dimethylaminocinnamaldehyde; D008787:Metoclopramide
keywords: Bromopride; Flow injection analysis; Pharmaceuticals; Urine