title: Simple multiplex real-time PCR for rapid detection of common 16S rRNA methyltransferase genes.
abstract: We have developed a real-time multiplex PCR assay to detect the three most common 16S rRNA methyltransferase genes (armA, rmtB and rmtC), which encode problematic high-level resistance to all clinically-relevant aminoglycoside antibiotics. All results were consistent with published conventional PCR assays and these genes still appear rare in Australia.
authors.affiliation: Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, New South Wales, Australia.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Guo
authors.affiliation: Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, New South Wales, Australia; Centre for Research Excellence in Critical Infection and Marie Bashir Institute for Emerging Infections and Biosecurity, University of Sydney, Sydney, New South Wales, Australia; Westmead Millennium Institute, Westmead, New South Wales, Australia.
authors.forename: Belinda B
authors.initials: BB
authors.lastname: Dillon
authors.affiliation: Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, New South Wales, Australia; Centre for Research Excellence in Critical Infection and Marie Bashir Institute for Emerging Infections and Biosecurity, University of Sydney, Sydney, New South Wales, Australia; Westmead Millennium Institute, Westmead, New South Wales, Australia.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Ginn
authors.affiliation: Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, New South Wales, Australia.
authors.forename: Agnieszka M
authors.initials: AM
authors.lastname: Wiklendt
authors.affiliation: Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, New South Wales, Australia; Centre for Research Excellence in Critical Infection and Marie Bashir Institute for Emerging Infections and Biosecurity, University of Sydney, Sydney, New South Wales, Australia; Westmead Millennium Institute, Westmead, New South Wales, Australia.
authors.forename: Sally R
authors.initials: SR
authors.lastname: Partridge
authors.affiliation: Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, New South Wales, Australia; Centre for Research Excellence in Critical Infection and Marie Bashir Institute for Emerging Infections and Biosecurity, University of Sydney, Sydney, New South Wales, Australia; Westmead Millennium Institute, Westmead, New South Wales, Australia. Electronic address: jonathan.iredell@sydney.edu.au.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Iredell
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D008780:Methyltransferases / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D008780:Methyltransferases
keywords: 16S rRNA methyltransferase; Aminoglycosides; Antibiotic resisance; real-time PCR