title: Whole-body cryostimulation as an effective way of reducing exercise-induced inflammation and blood cholesterol in young men.
abstract: Inflammation may accompany obesity and a variety of diseases, or result from excessive exercise. The aim of this study was to investigate the anti-inflammatory effect of whole-body cryostimulation on the inflammatory response induced by eccentric exercise under laboratory conditions. The study also sought to establish if cold treatment changes the lipid profile and modifies energy expenditure in young people. Eighteen healthy and physically active, college-aged men volunteered to participate in the experiment. They were divided into two subgroups: CRY- submitted to whole-body cryostimulation, and CONT- a control group. Both groups performed eccentric work to induce muscle damage. Blood samples were collected before and 24 h after the exercise. Over the five days that followed, the CRY group was exposed to a series of 10 sessions in a cryogenic chamber (twice a day, for 3 min, at a temperature of -110̊C). After this period of rest, both groups repeated a similar eccentric work session, following the same schedule of blood collection. The perceived pain was noted 24h after each session of eccentric workout. A 30-minute step up/down work-out induced delayed-onset muscle soreness in both groups. The five-day recovery period accompanied by exposure to cold significantly enhanced the concentration of the anti-inflammatory cytokine IL-10. It also led to a pronounced reduction in levels of the pro-inflammatory cytokine IL-1β, and reduced muscle damage. The values for IL-10 before the second bout of eccentric exercise in the CRY group were 2.0-fold higher in comparison to baseline, whereas in the CONT group, the concentration remained unchanged. Furthermore, blood concentrations of the pro-inflammatory cytokine IL-1β fell significantly in the CRY group. The main finding of this study was that a series of 10 sessions of whole body cryostimulation significantly reduced the inflammatory response induced by eccentric exercise. The lipid profile was also improved, but there was no effect on energy expenditure during the exercise.
authors.affiliation: Gdansk University of Physical Education and Sport, Department of Physiology, Kazimierza Gorskiego 1, Gdańsk, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Ziemann
authors.affiliation: Gdansk, University of Physical Education and Sport, Department of Biochemistry, Kazimierza Gorskiego 1, Gdańsk, Poland.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Olek
authors.affiliation: Gdansk University of Physical Education and Sport, Department of Physiology, Kazimierza Gorskiego 1, Gdańsk, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Grzywacz
authors.affiliation: Gdansk, University of Physical Education and Sport, Department of Physiotherapy, Kazimierza Gorskiego 1, Gdańsk, Poland.
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Kaczor
authors.affiliation: Medical University of Gdansk, Department of Bioenergetics and Physiology of Exercise, Marii Skłodowskiej-Curie 3A, Gdańsk, Poland.
authors.forename: Jędrzej
authors.initials: J
authors.lastname: Antosiewicz
authors.affiliation: Gdansk, University of Physical Education and Sport, Department of Physiotherapy, Kazimierza Gorskiego 1, Gdańsk, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Skrobot
authors.affiliation: Gdansk University of Physical Education and Sport, Department of Physiology, Kazimierza Gorskiego 1, Gdańsk, Poland.
authors.forename: Sylwester
authors.initials: S
authors.lastname: Kujach
authors.affiliation: Gdansk University of Physical Education and Sport, Department of Physiology, Kazimierza Gorskiego 1, Gdańsk, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Laskowski
mesh_terms: D000328:Adult; D002784:Cholesterol / Q000097:blood*; D003080:Cold Temperature; D017679:Cryotherapy / Q000379:methods*; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000473:pathology / Q000517:prevention & control / Q000628:therapy*; D018836:Inflammation Mediators / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D063806:Myalgia / Q000517:prevention & control / Q000628:therapy*; D010146:Pain / Q000517:prevention & control; D010147:Pain Measurement; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; C508585:IL1B protein, human; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D016753:Interleukin-10; D002784:Cholesterol
keywords: cold air exposure; cytokines; muscle damage