title: Detecting DNA depurination with solid-state nanopores.
abstract: Among the different types of DNA damage that occur endogenously in the cell, depurination is especially prevalent. These lesions can initiate mutagenesis and have been implicated in a variety of diseases, including cancer. Here, we demonstrate a new approach for the detection of depurination at the single-molecule scale using solid-state nanopores. We induce depurination in short duplex DNA using acidic conditions and observe that the presence of apurinic sites results in significantly slower dynamics during electrokinetic translocation. This procedure may be valuable as a diagnostic for in situ quantification of DNA depurination.
authors.affiliation: Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, North Carolina, United States of America.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Marshall
authors.affiliation: Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, North Carolina, United States of America.
authors.forename: Jan A
authors.initials: JA
authors.lastname: Ruzicka
authors.affiliation: Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, North Carolina, United States of America.
authors.forename: Ethan W
authors.initials: EW
authors.lastname: Taylor
authors.affiliation: Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, North Carolina, United States of America; Department of Biomedical Engineering, Wake Forest University School of Medicine, Winston-Salem, North Carolina, United States of America; Comprehensive Cancer Center, Wake Forest University School of Medicine, Winston-Salem, North Carolina, United States of America.
authors.forename: Adam R
authors.initials: AR
authors.lastname: Hall
mesh_terms: D001080:Apurinic Acid / Q000032:analysis*; D001483:Base Sequence; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D058608:Nanopores / Q000648:ultrastructure*; D011687:Purines / Q000032:analysis*
chemical_list: D011687:Purines; D001080:Apurinic Acid; D004247:DNA