title: Three puncture sites used for in utero electroporation show no significantly different negative impacts during gene transfer into the embryonic mouse brain.
abstract: Although various ways to manipulate genes in vivo exist, in utero electroporation is a widely used technique, especially in the field of neural development due to its many advantages. In this study, we focused on direct comparison between three puncture sites during in utero electroporation on the death rate of embryos, the thickness and the area of cortex, cell differentiation, cell proliferation, cell migration and cell apoptosis. We found no statistical significant differences between the three puncture methods in the death rate of embryos, the thickness and the area of cortex, cell differentiation, cell proliferation, cell migration and cell apoptosis.
authors.affiliation: Bayi Brain Hospital, Bayi Clinical Medical Institute of the Southern Medical University, Beijing 100700, China; Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China; Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China.
authors.forename: Cuiying
authors.initials: C
authors.lastname: Wu
authors.affiliation: Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Niu
authors.affiliation: Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Bayi Brain Hospital, Bayi Clinical Medical Institute of the Southern Medical University, Beijing 100700, China; Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China. Electronic address: zjxuruxiang@163.com.
authors.forename: Ruxiang
authors.initials: R
authors.lastname: Xu
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D004247:DNA / Q000008:administration & dosage*; D018274:Electroporation / Q000379:methods*; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques / Q000009:adverse effects*; D007267:Injections; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy
chemical_list: D004247:DNA
keywords: Differentiation; In utero electroporation; Proliferation; Puncture site; Thickness of cortex