title: Genetic and biological characterization of selected Changuinola viruses (Reoviridae, Orbivirus) from Brazil.
abstract: The genus Orbivirus of the family Reoviridae comprises 22 virus species including the Changuinola virus (CGLV) serogroup. The complete genome sequences of 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographical areas of the Brazilian Amazon region were obtained. All viral sequences were obtained from single-passaged CGLV strains grown in Vero cells. CGLVs are the only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure and molecular analysis by electron microscopy and gel electrophoresis, respectively, revealed viral particles with typical orbivirus size and morphology, as well as the presence of a segmented genome with 10 segments. Full-length nucleotide sequencing of each of the ten RNA segments of the 13 CGLV serotypes provided basic information regarding the genome organization, encoded proteins and genetic traits. Segment 2 (encoding VP2) of the CGLV is uncommonly larger in comparison to those found in other orbiviruses and shows varying sizes even among different CGLV serotypes. Phylogenetic analysis support previous serological findings, which indicate that CGLV constitutes a separate serogroup within the genus Orbivirus. In addition, six out of 13 analysed CGLV serotypes showed reassortment of their genome segments.
authors.affiliation: Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
authors.forename: Sandro P
authors.initials: SP
authors.lastname: Silva
authors.affiliation: Department of Virology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Meik
authors.initials: M
authors.lastname: Dilcher
authors.affiliation: Department of Virology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Weber
authors.affiliation: Department of Virology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Frank T
authors.initials: FT
authors.lastname: Hufert
authors.affiliation: Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.
authors.forename: Manfred
authors.initials: M
authors.lastname: Weidmann
authors.affiliation: Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
authors.forename: Jedson F
authors.initials: JF
authors.lastname: Cardoso
authors.affiliation: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
authors.forename: Valéria L
authors.initials: VL
authors.lastname: Carvalho
authors.affiliation: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
authors.forename: Jannifer O
authors.initials: JO
authors.lastname: Chiang
authors.affiliation: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
authors.forename: Lívia C
authors.initials: LC
authors.lastname: Martins
authors.affiliation: Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
authors.forename: Clayton P S
authors.initials: CPS
authors.lastname: Lima
authors.affiliation: Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
authors.forename: Daisy E A
authors.initials: DEA
authors.lastname: Da Silva
authors.affiliation: Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
authors.forename: João L S G
authors.initials: JLSG
authors.lastname: Vianez-Júnior
authors.affiliation: Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors.forename: Vsevolod L
authors.initials: VL
authors.lastname: Popov
authors.affiliation: Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors.forename: Amélia P A
authors.initials: APA
authors.lastname: Travassos da Rosa
authors.affiliation: Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
authors.affiliation: Departamento de Patologia, Universidade do Estado do Pará, Belém, Brazil.|Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
authors.forename: Pedro F C
authors.initials: PFC
authors.lastname: Vasconcelos
authors.affiliation: Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.
authors.forename: Márcio R T
authors.initials: MRT
authors.lastname: Nunes
mesh_terms: D000818:Animals; D001938:Brazil; D016000:Cluster Analysis; D004586:Electrophoresis; D023061:Gene Order; D016679:Genome, Viral; D006801:Humans; D007313:Insecta; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000032:analysis; D014771:Virion / Q000648:ultrastructure
chemical_list: D012367:RNA, Viral; D015678:Viral Structural Proteins