title: Serum and synovial fluid chemokine ligand 2/monocyte chemoattractant protein 1 concentrations correlates with symptomatic severity in patients with knee osteoarthritis.
abstract: OBJECTIVE
  Serum and synovial fluid (SF) chemokine ligand 2 (CCL2)/monocyte chemoattractant protein 1 (MCP-1) concentrations have been identified to be increased in osteoarthritis (OA) patients. The scope of this study was to examine CCL2 concentrations in serum and SF of knee OA patients and to explore their association with patient-reported symptomatic severity.
  
  
  METHODS
  One hundred and sixty-one knee OA patients and 138 healthy controls were enrolled into the study from our hospital. We collected Western Ontario and McMaster Universities Arthritis Index (WOMAC) scores from OA patients and measured CCL2 concentrations in serum and SF using enzyme-linked immunosorbent assay method, and correlation between WOMAC scores and CCL2 concentrations were analysed.
  
  
  RESULTS
  CCL2 concentrations in SF instead of serum were independently and positively associated with self-reported greater pain (r = 0.460, P < 0.001) and physical disability (r = 0.561, P < 0.001) in OA patients.
  
  
  CONCLUSIONS
  CCL2 in SF might serve as a novel and reliable biomarker for assessing symptomatic severity of OA.
authors.affiliation: Dong Ying People's Hospital, Dong Ying, China li_liang888888@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: Dong Ying People's Hospital, Dong Ying, China.
authors.forename: Bao-En
authors.initials: BE
authors.lastname: Jiang
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D018932:Chemokine CCL2 / Q000097:blood / Q000378:metabolism*; D002681:China; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000097:blood / Q000378:metabolism / Q000503:physiopathology*; D010147:Pain Measurement; D057566:Self Report; D012720:Severity of Illness Index; D013582:Synovial Fluid / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2
keywords: Osteoarthritis; chemokine ligand 2; monocyte chemoattractant protein 1; symptomatic severity