title: Sensitive detection of multiple mycotoxins by SPRi with gold nanoparticles as signal amplification tags.
abstract: Detection of multiple toxic mycotoxins is of importance in food quality control. Surface plasmon resonance imaging (SPRi) is an advanced tool for simultaneously multiple detections with accuracy; however, it suffers from limited sensitivity due to the instrumental constraint and small sizes of mycotoxins with only one epitope for an insensitive competitive immunoassay. In this work a gold nanoparticle (AuNP)-enhanced SPRi chip is designed to sensitively detect multiple mycotoxins using a competitive immunoassay format. The sensing surface is constructed by uniformly attaching dense mycotoxin antigens on poly[oligo(ethylene glycol) methacrylate-co-glycidyl methacrylate] (POEGMA-co-GMA) brush modified SPRi gold chip. After competitive binding in a sample solution containing respective monoclonal antibodies, secondary antibody-conjugated AuNPs are employed to bind with the captured monoclonal antibodies for further amplification of the SPRi signal. Highly specific and sensitive simultaneous detection is achieved for three typical mycotoxins including Aflatoxin B1 (AFB1), Ochratoxin A (OTA) and Zearalenone (ZEN) with low detection limits of 8, 30 and 15 pg mL(-1) and dynamic ranges covering three orders of magnitude.
authors.affiliation: Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing 400044, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Hu
authors.affiliation: Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Faculty of Materials and Energy, Southwest University, Chongqing 400715, China.
authors.forename: Hongming
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Faculty of Materials and Energy, Southwest University, Chongqing 400715, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Faculty of Materials and Energy, Southwest University, Chongqing 400715, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: He
authors.affiliation: Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Faculty of Materials and Energy, Southwest University, Chongqing 400715, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing 400044, China.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China; Faculty of Materials and Energy, Southwest University, Chongqing 400715, China. Electronic address: ecmli@swu.edu.cn.
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Li
mesh_terms: D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D009183:Mycotoxins / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D009183:Mycotoxins
keywords: Gold nanoparticles; Immunoassay; Mycotoxin; Signal amplification; Surface plasmon resonance imaging