title: Resveratrol attenuates hepatic steatosis in high-fat fed mice by decreasing lipogenesis and inflammation.
abstract: OBJECTIVE
  Resveratrol (RSV) is the most studied natural compound that activates sirtuins, which produce beneficial metabolic effects on lipid and glucose metabolism. The aim of the present study was to investigate the role of resveratrol in preventing nonalcoholic fatty liver disease (NAFLD) and expression of liver inflammatory markers in mice treated with a high-fat diet.
  
  
  METHODS
  Eighteen male mice were divided into three groups and fed for 60 d with a standard diet (ST), high-fat diet (HFD), or high-fat diet plus resveratrol (HFD + RSV, 30 mg/kg/d). Body weight, food intake, and serum total cholesterol, triacylglycerol, insulin, alanine transaminase (ALT), and aspartate aminotransferase (AST) were evaluated. Liver histology was analyzed. Expression of ACC, PPAR-γ, ChREBP, SREBP-1 c, CPT-1, tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), NF-κB, interleukin 1 β (IL-1 β), and SIRT1 were evaluated by quantitative real-time reverse transcriptase PCR (qRT-PCR).
  
  
  RESULTS
  The major finding of the present study was that RSV reduced body fat, total cholesterol, triacylglycerol, transaminases, and insulin plasma level. These results were accompanied with a significant reduction in TNF-α, IL-6, and NF-κB mRNA expression in the liver. Analyses of liver adipogenesis related genes indicated that ACC, PPAR-γ, and SREBP-1 mRNA expression were significantly suppressed in HFD + RSV mice. In addition, we observed increased expression of SIRT1 in the HFD + RSV group.
  
  
  CONCLUSIONS
  We observed that treatment with resveratrol improved lipid metabolism, and decreased NAFLD and pro-inflammatory profile in liver of mice with obesity-inducible diets. These data suggest an important clinical application of RSV in preventing liver diseases.
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: João Marcus Oliveira
authors.initials: JM
authors.lastname: Andrade
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Alanna Fernandes
authors.initials: AF
authors.lastname: Paraíso
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil; Nursing Department, Faculdades Integradas Pitágoras, Montes Claros, Minas Gerais, Brazil.
authors.forename: Marcos Vinícius Macedo
authors.initials: MV
authors.lastname: de Oliveira
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Andréa Maria Eleutério
authors.initials: AM
authors.lastname: Martins
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: João Felício
authors.initials: JF
authors.lastname: Neto
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: André Luiz Sena
authors.initials: AL
authors.lastname: Guimarães
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Alfredo Maurício
authors.initials: AM
authors.lastname: de Paula
authors.affiliation: Touro University Nevada College of Osteopathic and Medicine, Henderson, Las Vegas, Nevada, USA.
authors.forename: Mahboob
authors.initials: M
authors.lastname: Qureshi
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil; Institute of Biological Sciences, Department of Pharmacology, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: Sergiosousas@hotmail.com.
authors.forename: Sérgio Henrique Sousa
authors.initials: SH
authors.lastname: Santos
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D059305:Diet, High-Fat / Q000009:adverse effects*; D007249:Inflammation / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D050155:Lipogenesis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D009765:Obesity / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D000637:Transaminases / Q000097:blood
chemical_list: D016207:Cytokines; D007328:Insulin; D008055:Lipids; D016328:NF-kappa B; D010936:Plant Extracts; D012333:RNA, Messenger; D013267:Stilbenes; D000637:Transaminases; D000077185:Resveratrol
keywords: Inflammation; Nonalcoholic fatty liver disease; Obesity; Resveratrol; SIRT1