title: Influence of insulin-like growth factor I on nerve regeneration using allografts: a sciatic nerve model.
abstract: Effects of insulin-like growth factor I (IGF I) on peripheral nerve regeneration was studied using allografts in a rat sciatic nerve model. Thirty male white Wistar rats were divided into 3 experimental groups (n = 10) randomly: normal control group (NC), allograft group (ALLO), and IGF I-treated group (ALLO/IGF). In the NC group, the left sciatic nerve was exposed through a gluteal muscle incision and, after homeostasis, the muscle was sutured. In the ALLO group, the left sciatic nerve was exposed through a gluteal muscle incision and transected proximal to the tibioperoneal bifurcation where a 10-mm segment was excised. The same procedure was performed in the ALLO/IGF group. The harvested nerves of the rats of the ALLO group were served as allograft for the ALLO/IGF group and vice versa. The NC and ALLO groups received 10 μL of sterile phosphate buffered saline intraperitoneally once a day for 1 week, and the ALLO/IGF group received 10 μL of IGF I (100 ng/kg per day) intraperitoneally once a day for 1 week. Behavioral testing, sciatic nerve functional study and the gastrocnemius muscle mass showed earlier regeneration of axons in the ALLO/IGF group than in the ALLO group (P < 0.05). Administration of IGF I could accelerate functional recovery after nerve allografting in the sciatic nerve and may have clinical implications for the surgical management of patients after facial nerve transection.
authors.affiliation: From the Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
authors.forename: Abdolrahim
authors.initials: A
authors.lastname: Saadati
mesh_terms: D064591:Allografts / Q000637:transplantation*; D000818:Animals; D001369:Axons / Q000187:drug effects; D001522:Behavior, Animal; D002081:Buttocks / Q000294:innervation; D004195:Disease Models, Animal; D007334:Insulin-Like Growth Factor I / Q000627:therapeutic use*; D008297:Male; D018482:Muscle, Skeletal / Q000473:pathology; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D009929:Organ Size; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology; D012584:Sciatic Nerve / Q000637:transplantation*; D016138:Walking / Q000502:physiology
chemical_list: D007334:Insulin-Like Growth Factor I