title: Synthesis of poly(ester-carbonate) with a pendant acetylcholine analog for promoting neurite growth.
abstract: The modification of biodegradable polyesters with bioactive molecules has become an important strategy for controlling neuron adhesion and neurite outgrowth in nerve regeneration. In this study we report a biodegradable poly(ester-carbonate) with a pendant acetylcholine analog, which a neurotransmitter for the enhancement of neuron adhesion and outgrowth. The acetylcholine-functionalized poly(ester-carbonate) (Ach-P(LA-ClTMC)) was prepared by copolymerizing l-lactide (LA) and 5-methyl-5-chloroethoxycarbonyl trimethylene carbonate (ClTMC), followed by quaternization with trimethylamine. The acetylcholine analog content could be modulated by changing the molar feeding fraction of ClTMC. The incorporation of the acetylcholine analog improved the hydrophilicity of the films, but the acetylcholine analog content did not significantly influence the surface morphology of the acetylcholine-functionalized films. The results of PC12 cell culture showed that the acetylcholine analog promoted cell viability and neurite outgrowth in a concentration-dependent manner. The longest length of neurite and the percentage of cells bearing neurites were obtained on the Ach-P(LA-ClTMC)-10 film. All the results indicate that the integration of the acetylcholine analog at an appropriate fraction could be an effective strategy for optimizing the existing biodegradable polyesters for nerve regeneration applications.
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Dongming
authors.initials: D
authors.lastname: Xing
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: liema@zju.edu.cn.
authors.forename: Lie
authors.initials: L
authors.lastname: Ma
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: cygao@mail.hz.zj.cn.
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
mesh_terms: D000109:Acetylcholine / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D063566:Biodegradable Plastics / Q000138:chemical synthesis / Q000737:chemistry; D002448:Cell Adhesion; D002470:Cell Survival; D008567:Membranes, Artificial; D009416:Nerve Regeneration; D016501:Neurites / Q000378:metabolism*; D016716:PC12 Cells; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry; D051381:Rats
chemical_list: D063566:Biodegradable Plastics; D008567:Membranes, Artificial; D011091:Polyesters; D000109:Acetylcholine
keywords: Acetylcholine; Neurite outgrowth; PC12 cells; Poly(ester-carbonate)