title: NHE1 gene associated with avian leukosis virus subgroup J infection in chicken.
abstract: As a kind of binding protein, the type 1 Na(+)/H(+) exchanger (NHE1) is a receptor for the highly pathogenic Avian leukosis viruses-J subgroup (ALV-J) in chicken. In order to investigate the potential effect of chicken NHE1 gene on leukosis, we compared its expression between ALV-J-affected and -unaffected chicken, screened variations across the whole gene, and then performed association analysis with ALV-J affected/unaffected trait in three un-related chicken populations. We found that the NHE1 gene expressed in four immune tissues including spleen, bursa fabricius, liver, and thymus, and its expression was significantly up-regulated in liver and thymus of ALV-J-affected chickens (with leukosis phenotype) compared to -unaffected ones (ALV-J-negative controls). Thirty-six single nucleotide polymorphisms (SNP) were identified in a 6,105 bp region of the chicken NHE1 gene, giving rise to every 170 bp per SNP. Two SNP of g.4405A>G and g.5886C>G were genotyped with PCR-RFLP method. Results showed that g.4405A>G was significantly associated (P < 0.05) with ALV-J infection in all of the three chicken populations, including White Recessive Rock (WRR), Dwarf Yellow (DY) and Shiki Yellow (SY), while g.5886C>G was significantly associated (P < 0.05) with ALV-J infection in SY. These results indicated that the NHE1 gene was related to ALV-J infection in chicken.
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Chen
authors.forename: Weiling
authors.initials: W
authors.lastname: Pan
authors.forename: Liangyu
authors.initials: L
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Hongjia
authors.initials: H
authors.lastname: Ouyang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Nie
authors.forename: Xiquan
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D000818:Animals; D001353:Avian Leukosis / Q000235:genetics*; D001354:Avian Leukosis Virus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D002645:Chickens; D005260:Female; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D005838:Genotype; D020641:Polymorphism, Single Nucleotide; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics*
chemical_list: D017923:Sodium-Hydrogen Exchangers