title: A new 9-alkyladenine-cyclic methylglyoxal diadduct activates wt- and F508del-cystic fibrosis transmembrane conductance regulator (CFTR) in vitro and in vivo.
abstract: Cystic fibrosis transmembrane conductance regulator (CFTR) is the main chloride channel present in the apical membrane of epithelial cells and the F508 deletion (F508del-CFTR) in the CF gene is the most common cystic fibrosis-causing mutation. In the search for a pharmacotherapy of cystic fibrosis caused by the F508del-CFTR, a bi-therapy could be developed associating a corrector of F508del-CFTR trafficking and an activator of the channel activity of CFTR. Here, we report on the synthesis of 9-alkyladenine derivatives analogues of our previously discovered activator of wt-CFTR and F508del-CFTR, GPact-11a, and the identification of a new activator of these channels, GPact-26a, through various flux assays on human airway epithelial CF and non-CF cell lines and in vivo measurement of rat salivary secretion. This study reveals that the possible modifications of the side chain introduced at the N9 position of the main pharmacophore are highly limited since only an allyl group can replace the propyl side chain present in GPact-11a to lead to a strong activation of wt-CFTR in CHO cells. Docking simulations of the synthesised compounds and of four described modulators performed using a 3D model of the wt-type CFTR protein suggest five possible binding sites located at the interface of the nucleotide binding domains NBD1/NBD2. However, the docking study did not allow the differentiation between active and non-active compounds.
authors.affiliation: Université Grenoble Alpes, Joseph Fourier/CNRS, UMR 5063, Département de Pharmacochimie Moléculaire, 470 rue de la Chimie, F-38041 Grenoble, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Boucherle
authors.affiliation: Université de Poitiers/CNRS, Laboratoire Signalisation et Transports Ioniques Membranaires, 1 rue Georges Bonnet, F-86022 Poitiers, France.
authors.forename: Johanna
authors.initials: J
authors.lastname: Bertrand
authors.affiliation: Université Grenoble Alpes, Joseph Fourier/CNRS, UMR 5063, Département de Pharmacochimie Moléculaire, 470 rue de la Chimie, F-38041 Grenoble, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Maurin
authors.affiliation: Université Grenoble Alpes, Joseph Fourier/CNRS, UMR 5063, Département de Pharmacochimie Moléculaire, 470 rue de la Chimie, F-38041 Grenoble, France.
authors.forename: Brice-Loïc
authors.initials: BL
authors.lastname: Renard
authors.affiliation: Université Grenoble Alpes, Joseph Fourier/CNRS, UMR 5063, Département de Pharmacochimie Moléculaire, 470 rue de la Chimie, F-38041 Grenoble, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Fortuné
authors.affiliation: Université de Poitiers/CNRS, Laboratoire Signalisation et Transports Ioniques Membranaires, 1 rue Georges Bonnet, F-86022 Poitiers, France.
authors.forename: Brice
authors.initials: B
authors.lastname: Tremblier
authors.affiliation: Université de Poitiers/CNRS, Laboratoire Signalisation et Transports Ioniques Membranaires, 1 rue Georges Bonnet, F-86022 Poitiers, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Becq
authors.affiliation: Université de Poitiers/CNRS, Laboratoire Signalisation et Transports Ioniques Membranaires, 1 rue Georges Bonnet, F-86022 Poitiers, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Norez
authors.affiliation: Université Grenoble Alpes, Joseph Fourier/CNRS, UMR 5063, Département de Pharmacochimie Moléculaire, 470 rue de la Chimie, F-38041 Grenoble, France. Electronic address: jean-luc.decout@ujf-grenoble.fr.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Décout
mesh_terms: D000225:Adenine / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015195:Drug Design; D006801:Humans; D008297:Male; D008958:Models, Molecular; D011487:Protein Conformation; D051381:Rats; D017384:Sequence Deletion
chemical_list: D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000225:Adenine
keywords: Activators; Cystic fibrosis; Docking; F508del-CFTR; Modulators; Nucleotide binding domains; Salivary secretion