title: Fabrication of glycopolymer/MWCNTs composite nanofibers and its enzyme immobilization applications.
abstract: Glycopolymer (poly(AN-co-OVSEG))/MWCNTs (multiwalled carbon nanotubes) composite nanofibers are fabricated using a facile approach combining enzymatic synthesis, radical polymerization and electrospinning. The structure of the glycopolymer was confirmed by FT-IR and (1)H NMR. Poly(AN-co-OVSEG)/MWCNTs composite nanofibers were prepared using electrospinning and characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The hydrophilic properties of the composite nanofibers surfaces were increased since the contact angle of poly(AN-co-OVSEG)/MWCNTs composite was reduced from 65.5° to 37° compared to (PAN). As an enzymatic model catalase (CAT) was loaded (ca. 55.0mg/g) to the poly(AN-co-OVSEG)/MWCNTs nanofibers. The optimum temperature for poly(AN-co-OVSEG)/MWCNTs nanofibers increased from 25°C to 45°C compared to free CAT. The covalently immobilized enzymes conjugate exhibited 60% activity at 60°C, while the free enzyme was entirely inactivity after 5min heat treatment. The immobilized CAT retained 70% of its initial activity after 5 cycles of decomposition of hydrogen peroxide.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Quan
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Zhongqing
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute for Health Research and Policy, London Metropolitan University, London N7 8DB, UK.
authors.forename: Christopher
authors.initials: C
authors.lastname: Branford-White
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Huali
authors.initials: H
authors.lastname: Nie
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: lzhu@dhu.edu.cn.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism*; D002417:Cattle; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D059027:Recycling; D013696:Temperature; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D011108:Polymers; D011134:Polysaccharides; D002374:Catalase
keywords: CAT immobilization; Composite Nanofibers; Electrospun; Glycopolymer; MWCNTs