title: Single gold nanoplasmonic sensor for clinical cancer diagnosis based on specific interaction between nucleic acids and protein.
abstract: Plasmonic nanomaterials reveal noble optical properties for next-generation biosensors. Nanoplasmonic biosensors have become simple, sensitive, smart, and consistent with advanced healthcare programs requirements. Notably, an individual nanoparticle analysis can yield unique target information, based on which the next-generation biosensor is revolutionary for end-point detection (single or multiplex), and can be functionally extended to biological phenomena monitoring. Here, we present a single nanoplasmonic sensing technology based on localized surface plasmon resonance for label-free and real-time detection of highly reliable cancer markers (mutant gene and telomerase) in clinical samples. The sensor specifically detects mutant DNA, and can detect telomerase from as few as 10 HeLa cells. This approach can be easily translated to detect other pathological targets with high sensitivity and specificity, and monitor key interactions between biomolecules such as nucleic acids and proteins during disease development in real time. This system has great potential to be further developed for on-chip and simultaneous analysis of multiple targets and interactions.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea.
authors.forename: Xingyi
authors.initials: X
authors.lastname: Ma
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Phuoc Long
authors.initials: PL
authors.lastname: Truong
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea.
authors.forename: Nguyen Hung
authors.initials: NH
authors.lastname: Anh
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea. Electronic address: simsj@korea.ac.kr.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Sim
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000302:isolation & purification*; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics; D009696:Nucleic Acids / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D020349:Surface Plasmon Resonance; D019098:Telomerase / Q000302:isolation & purification
chemical_list: D014408:Biomarkers, Tumor; D009696:Nucleic Acids; D011506:Proteins; D006046:Gold; D019098:Telomerase
keywords: Cancer cell; DNA mutation; Gold nanoparticle (AuNP); Localized surface plasmon resonance (LSPR); Nanoplasmonic sensor; Telomerase activity