title: Aptamer-based competitive binding assay for one-step quantitation of hepatitis B surface antigen.
abstract: An aptamer-based competitive binding assay for one-step (i.e. no requirement of pre-treatment) quantitation of target molecules of interest has been developed. This method has been successfully employed for the fast and sensitive detection of the surface antigen of the hepatitis B virus (HBsAg). The key features of our method include its low intrinsic background noise, low costs, high resolution, and high sensitivity, enabling the detection of as low as 1.25 mIU mL(-1), approximately 40-fold better than that of the most widely used Abbott Architect assay for HBsAg detection, without the tedious extraction and/or washing procedures. Moreover, this assay has better recovery and accuracy than that of conventional competitive binding assay or others for HBsAg quantitation.
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Korea. sshah@khu.ac.kr.
authors.forename: Sung-Kwan
authors.initials: SK
authors.lastname: Suh
authors.forename: Seongeun
authors.initials: S
authors.lastname: Song
authors.forename: Heung-Bum
authors.initials: HB
authors.lastname: Oh
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Hwang
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Hah
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001667:Binding, Competitive; D002232:Carbocyanines / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000097:blood*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection
chemical_list: D052157:Aptamers, Nucleotide; D002232:Carbocyanines; D006514:Hepatitis B Surface Antigens; C089187:cyanine dye 3