title: Purification and characterization of a cold-adapted lipase from Oceanobacillus strain PT-11.
abstract: We isolated a moderately halophilic lipase-producing bacterium from the saline soil. Based on the morphological, physiological, chemotaxonomic and phylogenetic analysis, the isolate PT-11 was postulated to be a novel species identified as Oceanobacillus rekensis PT-11. The lipase was purified 2.50-fold by Q-Sepharose FF and SP-Sepharose FF chromatography and its molecular mass was estimated to be 23.5 kDa by SDS-PAGE. It was highly active over the broad temperature ranging from 10 to 35°C and showed up to 80% of the maximum activity at 10°C indicating the lipase to be a typical cold-adapted enzyme. The enzyme activity was slightly enhanced by Na+, Li+ and K+. Incubation with detergents, such as Tween-20 and Tween-80, slightly inhibited the enzyme activity; while Triton X-100decreased the enzyme activity. The enzyme was fairly stable in the presence of long-chain alcohols but was highly denatured in hydrophilic solvents such as acetone or short-chain alcohols (C1-C3).
authors.affiliation: Key laboratory of Leather Chemistry and engineering, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu, China; Department of Pharmaceutical and Biological Engineering, College of Chemical Engineering, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Jiewei
authors.affiliation: Department of Pharmaceutical and Biological Engineering, College of Chemical Engineering, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zuchao
authors.affiliation: Department of Pharmaceutical and Biological Engineering, College of Chemical Engineering, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Department of Pharmaceutical and Biological Engineering, College of Chemical Engineering, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Lei
authors.affiliation: Key laboratory of Leather Chemistry and engineering, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Yongqiang
mesh_terms: D001406:Bacillaceae / Q000201:enzymology* / Q000302:isolation & purification; D002847:Chromatography, Agarose; D003080:Cold Temperature; D003902:Detergents; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight
chemical_list: D003902:Detergents; D008049:Lipase