title: WASH complex regulates Arp2/3 complex for actin-based polar body extrusion in mouse oocytes.
abstract: Prior to their fertilization, oocytes undergo asymmetric division, which is regulated by actin filaments. Recently, WASH complex were identified as actin nucleation promoting factors (NPF) that activated Arp2/3 complex. However, the roles of WASH complex remain uncertain, particularly for oocyte polarization and asymmetric division. Here, we examined the functions of two important subunits of a WASH complex, WASH1 and Strumpellin, during mouse oocyte meiosis. Depleting WASH1 or disrupting Strumpellin activity by WASH1 morpholino (MO) injection or Strumpellin antibody injection decreased polar body extrusion and caused oocyte symmetric division, and this may have been due to spindle formation and migration defects. Time lapse microscopy showed that actin filaments distribution and relative amount at the membrane and in the cytoplasm of oocytes was significantly decreased after disrupting WASH complex. In addition, Arp2/3 complex expression was reduced after WASH1 depletion. Thus, our data indicated that WASH complex regulated Arp2/3 complex and were required for cytokinesis and following polar body extrusion during mouse oocyte meiotic maturation.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhen-Bo
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea.
authors.forename: Xiang-Shun
authors.initials: XS
authors.lastname: Cui
authors.affiliation: Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea.
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Shao-Chen
authors.initials: SC
authors.lastname: Sun
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D048749:Cytokinesis; D005260:Female; D008540:Meiosis; D008813:Mice, Inbred ICR; D008840:Microfilament Proteins / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D059705:Polar Bodies / Q000502:physiology*; D021381:Protein Transport; D008941:Spindle Apparatus / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000502:physiology*
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D008840:Microfilament Proteins; D033921:Vesicular Transport Proteins; C577014:WASH protein, mouse