title: Stiff person-syndrome IgG affects presynaptic GABAergic release mechanisms.
abstract: The majority of patients with stiff person-syndrome (SPS) are characterized by autoantibodies to glutamate decarboxylase 65 (GAD65). In previous passive-transfer studies, SPS immunoglobulin G (IgG) induced SPS core symptoms. We here provide evidence that SPS-IgG causes a higher frequency of spontaneous vesicle fusions. Sustained GABAergic transmission and presynaptic GABAergic vesicle pool size remained unchanged. Since these findings cannot be attributed to anti-GAD65 autoantibodies alone, we propose that additional autoantibodies with so far undefined antigen specificity might affect presynaptic release mechanisms.
authors.affiliation: Hans-Berger Department of Neurology, Jena University Hospital, Erlanger Allee, 07747, Jena, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Werner
authors.forename: Holger
authors.initials: H
authors.lastname: Haselmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Weishaupt
authors.forename: Klaus V
authors.initials: KV
authors.lastname: Toyka
authors.forename: Claudia
authors.initials: C
authors.lastname: Sommer
authors.forename: Christian
authors.initials: C
authors.lastname: Geis
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005260:Female; D059330:GABAergic Neurons / Q000166:cytology* / Q000187:drug effects; D005968:Glutamate Decarboxylase / Q000276:immunology*; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology*; D066298:In Vitro Techniques; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D018377:Neurotransmitter Agents / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000187:drug effects* / Q000378:metabolism; D018709:Statistics, Nonparametric; D016750:Stiff-Person Syndrome / Q000276:immunology / Q000378:metabolism*; D016708:Synaptophysin / Q000378:metabolism; D050495:Vesicular Inhibitory Amino Acid Transport Proteins / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D018377:Neurotransmitter Agents; D016708:Synaptophysin; D050495:Vesicular Inhibitory Amino Acid Transport Proteins; C492244:Viaat protein, mouse; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2