title: Study of an antiangiogenesis gene therapy with endostatin on endometriosis in the nude mouse model.
abstract: OBJECTIVE
  This work aims to investigate the treatment effect of endostatin (ES) in the nude mouse model with endometriosis (EMs).
  
  
  METHODS
  Recombinant adenovirus Ad-ES carrying ES gene was constructed. Apoptosis of ECV-304 cell induced by Ad-ES was observed. The nude mouse model with EMs was established by subcutaneous implantation. After the local focus was injected with the Ad-ES, the Ad-Track or the physiologic saline, respectively, the morphological features of ectopic focuses were observed under microscopy. The microvessel densities (MVD) and the apoptosis were detected.
  
  
  RESULTS
  The recombinant Ad-ES was successfully constructed. Apoptosis of ECV 304 cells could be induced by Ad-ES. The nude mouse model with EMs was successfully established by subcutaneous implantation. There were statistical differences in the volumes of endometriotic lesions and MVD after treatment by Ad-ES compared with those in the other two control groups (p < 0.05). Apoptosis of the cells were significantly increased in the group of treatment by Ad-ES compared with those of the two control groups.
  
  
  CONCLUSIONS
  ES could induce ECV 304 cells to apoptosis and inhibit the growth ofendometrium in the nude mouse model. The findings suggest that antiangiopoiesis may be used as a promising therapy for the treatment of EMs.
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
authors.forename: Y L
authors.initials: YL
authors.lastname: He
mesh_terms: D000256:Adenoviridae; D000328:Adult; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D004195:Disease Models, Animal; D004715:Endometriosis / Q000235:genetics / Q000628:therapy*; D043169:Endostatins / Q000235:genetics*; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D055806:Microvessels; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000628:therapy*; D010957:Plasmids; D011995:Recombination, Genetic
chemical_list: D043169:Endostatins