title: Live imaging of baculovirus infection of midgut epithelium cells: a functional assay of per os infectivity factors.
abstract: The occlusion-derived viruses (ODVs) of baculoviruses are responsible for oral infection of insect hosts, whereas budded viruses (BVs) are responsible for systemic infection within the host. The ODV membrane proteins play crucial roles in mediating virus entry into midgut epithelium cells to initiate infection and are important factors in host-range determination. For Autographa californica multiple nucleopolyhedrovirus (AcMNPV), seven conserved ODV membrane proteins have been shown to be essential for oral infectivity and are called per os infectivity factors (PIFs). Information on the function of the individual PIF proteins in virus entry is limited, partly due to the lack of a good in vitro system for monitoring ODV entry. Here, we constructed a baculovirus with EGFP fused to the nucleocapsid to monitor virus entry into primary midgut epithelium cells ex vivo using confocal fluorescence microscopy. The EGFP-labelled virus showed similar BV virulence and ODV infectivity as WT virus. The ability to bind and enter host cells was then visualized for WT AcMNPV and viruses with mutations in P74 (PIF0), PIF1 or PIF2, showing that P74 is required for ODV binding, whilst PIF1 and PIF2 play important roles in the entry of ODV after binding to midgut cells. This is the first live imaging of ODV entry into midgut cells and complements the genetic and biochemical evidence for the role of PIFs in the oral infection process.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, Hubei Province, PR China.|Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Mu
authors.affiliation: Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Jan W M
authors.initials: JWM
authors.lastname: van Lent
authors.affiliation: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
authors.forename: Guy
authors.initials: G
authors.lastname: Smagghe
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, Hubei Province, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, Hubei Province, PR China.
authors.forename: Xinwen
authors.initials: X
authors.lastname: Chen
authors.affiliation: Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Just M
authors.initials: JM
authors.lastname: Vlak
authors.affiliation: Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Monique M
authors.initials: MM
authors.lastname: van Oers
mesh_terms: D000818:Animals; D004064:Digestive System / Q000821:virology; D004847:Epithelial Cells / Q000821:virology; D049452:Green Fluorescent Proteins / Q000235:genetics; D007181:Inclusion Bodies, Viral / Q000502:physiology; D009154:Mutation; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D061987:Sf9 Cells; D018411:Spodoptera; D014759:Viral Envelope Proteins / Q000502:physiology; D014774:Virulence / Q000235:genetics / Q000502:physiology; D037521:Virulence Factors / Q000502:physiology; D053586:Virus Internalization
chemical_list: D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D037521:Virulence Factors; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins