title: Treatment of life-threatening wounds with a combination of allogenic platelet-rich plasma, fibrin glue and collagen matrix, and a literature review.
abstract: Currently there is no ideal procedure for the treatment of recalcitrant ulcers that are unresponsive to the majority of common treatments. However, several novel approaches have been proposed, including bone marrow stem cells, platelets, fibrin glue and collagen matrix. For the first approach treatment of a chronic wound, a non-invasive method is highly desirable. The present study was undertaken with the aim of evaluating the effect of a combination of platelets, fibrin glue and collagen matrix (PFC) in one treatment. A total of ten patients with aggressive, refractory, life-threatening wounds were recruited for the study and their treatment effects were evaluated. Initially, the ulcers were extensively debrided, measured and photographed at weekly intervals. The PFC combination was applied topically to the wound every two days. Following treatment, the wound was completely closed in nine patients and was markedly reduced in the other patient. The mean 100% healing time for the nine patients was 11.3±5.22 weeks. There was no evidence of local or systemic complications or any abnormal tissue formation, keloid or hypertrophic scarring. Therefore, the results of the present study indicate that in the first approach, the combination of PFC components may be used safely in order to synergize the effect of chronic wound healing.
authors.affiliation: Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Asadi
authors.affiliation: Stem Cell and Regenerative Medicine Research Group, Department of Biochemistry, Stem Cell Laboratory, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
authors.forename: Daryoush Hamidi
authors.initials: DH
authors.lastname: Alamdari
authors.affiliation: Student Research Committee, Department of Modern Sciences and Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Rahimi
authors.affiliation: Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Aliakbarian
authors.affiliation: Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jangjoo
authors.affiliation: Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Abdollahi
authors.affiliation: Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
authors.forename: Mostafa Mehrabi
authors.initials: MM
authors.lastname: Bahar
authors.affiliation: Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Azadmand
authors.affiliation: Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
authors.forename: Naser
authors.initials: N
authors.lastname: Forghani
authors.affiliation: Stem Cell and Regenerative Medicine Research Group, Department of Biochemistry, Stem Cell Laboratory, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 917794-8564, Iran.
authors.forename: Mohammad Nori
authors.initials: MN
authors.lastname: Sadegh
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Mashhad 91875, Iran.
authors.forename: Mohammad Esmail
authors.initials: ME
authors.lastname: Khayamy
authors.affiliation: UCL Centre for Nanotechnology and Regenerative Medicine, Division of Surgery and Interventional Science, University College London, Royal Free Hampstead NHS Trust Hospital, London, UK.
authors.forename: Alexander
authors.initials: A
authors.lastname: Seifalian
keywords: collagen matrix; fibrin glue; platelet-rich plasma; recalcitrant wounds