title: Endothelial Nitric Oxide Synthase Gene T-786C Polymorphism in Renal Transplant Recipients.
abstract: BACKGROUND
  Nitric oxide (NO) is a major mediator in vascular biology, regulating regional blood flow. NO and the enzymes required for its production contribute to ischemia-reperfusion injury. The T-786C functional polymorphism in the promoter region substantially reduces promoter activity of the endothelial nitric oxide synthase (eNOS) gene and compromises endothelial NO synthesis.
  
  
  OBJECTIVE
  To examine the association between T-786C (rs 2070744) single nucleotide polymorphism (SNP) in eNOS gene and the development of acute rejection in renal transplant patients.
  
  
  METHODS
  60 renal transplant recipients (30 with episodes of acute rejection (ARs) and 30 without rejection (non-ARs)), between June 2008 and March 2010, were included in this study. The polymorphism was determined by PCR-restriction fragment-length polymorphism analysis.
  
  
  RESULTS
  The distribution of the genotypes were TT/TC/CC 60%, 33.4%, 6.6%, and 43%, 46.7%, 13.3% in ARs and non-ARs, respectively (p=0.28). The frequency of T-allele was 76.7% and 66.3%; and for C-allele was 66.6% and 33.3% in ARs and non-ARs, respectively (p=0.09). There were no significant associations between these polymorphisms and acute and chronic kidney allograft rejection.
  
  
  CONCLUSIONS
  We could not detect any significant association between polymorphism in T-786C of eNOS gene and the development of acute rejection.
authors.affiliation: Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Azarpira
authors.affiliation: Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran.
authors.forename: B
authors.initials: B
authors.lastname: Geramizadeh
authors.affiliation: Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran ; Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Nikeghbalian
authors.affiliation: Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Bahador
authors.affiliation: Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Yaghobi
authors.affiliation: Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Karimi
authors.affiliation: Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Ayatolahi
authors.affiliation: Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran.
authors.forename: M H
authors.initials: MH
authors.lastname: Aghdai
authors.affiliation: Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Salahi
authors.affiliation: Shiraz Transplant Research Center, University of Medical Sciences, Shiraz, Iran ; Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran.
authors.forename: S A
authors.initials: SA
authors.lastname: Malek-Hosseini
authors.affiliation: Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran.
authors.forename: J
authors.initials: J
authors.lastname: Roozbeh
authors.affiliation: Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Sagheb
authors.affiliation: Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran.
authors.forename: G H
authors.initials: GH
authors.lastname: Raisjalali
authors.affiliation: Organ Transplant Center, Shiraz , University of Medical Sciences, Shiraz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Behzadi
keywords: Acute rejection; Endothelial nitric oxide synthase; Nitric oxide; Polymorphism; Transplant