title: UPLC-MS/MS method for determination of avicularin in rat plasma and its application to a pharmacokinetic study.
abstract: A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of avicularin in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2 mL of acetonitrile-methanol (9:1, v/v) to a 0.1 mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 1.60 min and the elution of avicularin was at 1.20 min. The detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction-monitoring (MRM) mode using the respective transitions m/z 434.1→301.3 for avicularin and m/z 237.2→194.3 for carbamazepine (IS), respectively. The calibration curve was linear over the range of 10-3000 ng/mL with a lower limit of quantitation (LLOQ) of 10 ng/mL. Mean recovery of avicularin in plasma was in the range of 84.2-89.5%. Intra-day and inter-day precision were both <12%. This method was successfully applied in pharmacokinetic study after intravenous administration of 5.0mg/kg avicularin in rats.
authors.affiliation: The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, PR China. Electronic address: 13513792468@139.com.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhang
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Rui-Fang
authors.initials: RF
authors.lastname: Li
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Da-Ming
authors.initials: DM
authors.lastname: Hu
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Jian-Fei
authors.initials: JF
authors.lastname: Qiu
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.
authors.forename: Yun-Hao
authors.initials: YH
authors.lastname: Yan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005419:Flavonoids; C041388:avicularin
keywords: Avicularin; Pharmacokinetics; Rat plasma; UPLC–MS/MS