title: Transcriptional activity assessment of three different promoters for mouse in utero electroporation system.
abstract: In utero electroporation (IUE) is a simple and rapid approach to in vivo investigate exogenous gene function in mouse brain, and intensive studies using IUE have greatly contributed to analyze the characterization of specific steps during mouse brain development. Because the efficiency of IUE is highly dependent on the plasmid used and its concentration, and the transcriptional activity of plasmid is not only regulated by the host defense system, but also by the promoter of the expression vectors. Therefore, in the present study, we evaluated the transcriptional activity of three commonly used promoters, CMV, CAG and SV40, in IUE system through measuring the fluorescence intensity of green fluorescent protein which serves as an indicator. Our results demonstrated that the artificially-designed CAG promoter is a potent promoter that effectively drives target gene expression in IUE system.
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, 453003 Xinxiang, China; Key Lab for Medical Tissue Regeneration of Henan Province, 453003 Xinxiang, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, 453003 Xinxiang, China.
authors.forename: Sulei
authors.initials: S
authors.lastname: Fu
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, 453003 Xinxiang, China.
authors.forename: Rongcheng
authors.initials: R
authors.lastname: Niu
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, 453003 Xinxiang, China; Key Lab for Medical Tissue Regeneration of Henan Province, 453003 Xinxiang, China.
authors.forename: Ciqing
authors.initials: C
authors.lastname: Yang
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, 453003 Xinxiang, China; Key Lab for Medical Tissue Regeneration of Henan Province, 453003 Xinxiang, China; Institute of Anatomy I, Jena University Hospital, Teichgraben 7, 07743 Jena, Germany. Electronic address: juntang.lin@googlemail.com.
authors.forename: Juntang
authors.initials: J
authors.lastname: Lin
mesh_terms: D000818:Animals; D018274:Electroporation / Q000379:methods*; D005260:Female; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D061848:Optical Imaging; D010957:Plasmids / Q000235:genetics*; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation / Q000235:genetics*; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins
keywords: Brain development; Green fluorescent protein; In utero electroporation; Promoter