title: Construction of tissue-engineered bone using a bioreactor and platelet-rich plasma.
abstract: The aim of the present study was to construct tissue-engineered bone using a bioreactor and platelet-rich plasma (PRP). Bone marrow mesenchymal stem cells (BMSCs) and β-tricalcium phosphate (β-TCP) were cultured in a perfusion bioreactor with PRP-containing medium for 21 days to form a BMSC-TCP composite. Rabbits were then implanted with the BMSC-TCP composite. The morphology of the implanted BMSC-TCP composite was observed three months after surgery by scanning electron microscopy and hematoxylin and eosin (H&E) staining. In addition, the expression of cluster of differentiation (CD)31 and von Willebrand factor (WF) in the implanted BMSC-TCP composite was detected using immunohistochemistry. Bone formation was determined by comprehensive testing Following culture in a perfusion bioreactor and PRP, the BMSCs adhered to the β-TCP scaffold and the secretion of extracellular matrix was observed. The spreading and proliferation of cells was found to be enhanced on the scaffold. Furthermore, the vascular endothelial cell markers CD31 and VEF, were positively expressed. Therefore, these results suggest that tissue-engineered bone may be constructed using a bioreactor and PRP. PRP, which contains multiple growth factors, may promote vascularization of tissue-engineered bone.
authors.affiliation: Department of Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China.
authors.forename: Honglei
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Equipment, The People's Hospital of Laiwu, Laiwu, Shandong 271100, P.R. China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China.
authors.forename: Huibo
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China.
authors.forename: Huawu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: Department of Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Department of Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China.
authors.forename: Shui
authors.initials: S
authors.lastname: Sun
keywords: perfusion bioreactor; platelet-rich plasma; rabbit bone marrow mesenchymal stem cells; β-tricalcium phosphate