title: Validation of an enzyme-linked immunosorbent assay that detects Histoplasma capsulatum antigenuria in Colombian patients with AIDS for diagnosis and follow-up during therapy.
abstract: We validated an antigen capture enzyme-linked immunosorbent assay (ELISA) in Colombian persons with AIDS and proven histoplasmosis and evaluated the correlation between antigenuria and clinical improvement during follow-up. The sensitivity of the Histoplasma capsulatum ELISA was 86%, and the overall specificity was 94%. The antigen test successfully monitored the response to therapy.
authors.affiliation: Corporación para Investigaciones Biológicas (CIB), Medical and Experimental Mycology Group, Medellín, Colombia.
authors.forename: Diego H
authors.initials: DH
authors.lastname: Caceres
authors.affiliation: Centers for Disease Control and Prevention, Mycotic Diseases Branch, Atlanta, Georgia, USA.
authors.forename: Christina M
authors.initials: CM
authors.lastname: Scheel
authors.affiliation: Corporación para Investigaciones Biológicas (CIB), Medical and Experimental Mycology Group, Medellín, Colombia Hospital La María, Medellín, Colombia.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Tobón
authors.affiliation: Centers for Disease Control and Prevention, Mycotic Diseases Branch, Atlanta, Georgia, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Ahlquist Cleveland
authors.affiliation: Corporación para Investigaciones Biológicas (CIB), Medical and Experimental Mycology Group, Medellín, Colombia.
authors.forename: Angela
authors.initials: A
authors.lastname: Restrepo
authors.affiliation: Centers for Disease Control and Prevention, Mycotic Diseases Branch, Atlanta, Georgia, USA.
authors.forename: Mary E
authors.initials: ME
authors.lastname: Brandt
authors.affiliation: Centers for Disease Control and Prevention, Mycotic Diseases Branch, Atlanta, Georgia, USA.
authors.forename: Tom
authors.initials: T
authors.lastname: Chiller
authors.affiliation: Corporación para Investigaciones Biológicas (CIB), Medical and Experimental Mycology Group, Medellín, Colombia Universidad del Rosario, School of Medicine and Health Sciences, BogotÁ, Colombia beatrizlgomez@hotmail.com.
authors.forename: Beatriz L
authors.initials: BL
authors.lastname: Gómez
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis; D000946:Antigens, Fungal / Q000652:urine*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D015331:Cohort Studies; D003105:Colombia; D016903:Drug Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006658:Histoplasma / Q000276:immunology*; D006660:Histoplasmosis / Q000175:diagnosis* / Q000188:drug therapy; D006801:Humans; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D000946:Antigens, Fungal