title: Antioxidative properties of 4-methylumbelliferone are related to antibacterial activity in the silkworm (Bombyx mori) digestive tract.
abstract: Umbelliferones have gained significant attention due to their tumor-inhibitory effects in vitro. This study was undertaken to examine the impact of umbelliferones in an invertebrate model organism, Bombyx mori, to assess the underlying antimicrobial activities via antioxidation in vivo. Oral administration of 4 mM 4-methylumbelliferone (4-MU), a model umbelliferone drug, in B. Mori larvae caused a rapid increase in reactive oxygen species, such as hydrogen peroxide (H2O2) and antimicrobial activity in the digestive tract. In addition, a significant increase in total antioxidant capacity as well as superoxide anion radical-inhibiting activity and reduced glutathione were detected. The antioxidant defense system was activated following induction of H2O2, resulting in a significant rise in catalase (50-66 %) and glutathione peroxidase (175 %) activities, which were helpful in defending digestive tract cells against oxidative injury. These results help in understanding the anticancer mechanism of 4-MU based on its antioxidation in organisms.
authors.affiliation: Department of Applied Biology, School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, 215123, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhu
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Hengjiang
authors.initials: H
authors.lastname: Liu
authors.forename: Xu
authors.initials: X
authors.lastname: Xu
authors.forename: Weimin
authors.initials: W
authors.lastname: Yin
authors.forename: Yanghu
authors.initials: Y
authors.lastname: Sima
authors.forename: Shiqing
authors.initials: S
authors.lastname: Xu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D012831:Bombyx; D002374:Catalase / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000378:metabolism*; D020869:Gene Expression Profiling; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006923:Hymecromone / Q000008:administration & dosage / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000037:antagonists & inhibitors
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D013481:Superoxides; D006923:Hymecromone; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione