title: Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes.
abstract: For the first time, respiratory complex I has been reconstituted on an electrode preserving its structure and activity. Respiratory complex I is a membrane-bound enzyme that has an essential function in cellular energy production. It couples NADH:quinone oxidoreduction to translocation of ions across the cellular (in prokaryotes) or mitochondrial membranes. Therefore, complex I contributes to the establishment and maintenance of the transmembrane difference of electrochemical potential required for adenosine triphosphate synthesis, transport, and motility. Our new strategy has been applied for reconstituting the bacterial complex I from Rhodothermus marinus onto a biomimetic membrane supported on gold electrodes modified with a thiol self-assembled monolayer (SAM). Atomic force microscopy and faradaic impedance measurements give evidence of the biomimetic construction, whereas electrochemical measurements show its functionality. Both electron transfer and proton translocation by respiratory complex I were monitored, simulating in vivo conditions.
authors.affiliation: Instituto de Catalisis y Petroleoquímica, CSIC, c/Marie Curie 2, L10, 28049 Madrid, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Gutiérrez-Sanz
authors.forename: David
authors.initials: D
authors.lastname: Olea
authors.forename: Marcos
authors.initials: M
authors.lastname: Pita
authors.forename: Ana P
authors.initials: AP
authors.lastname: Batista
authors.forename: Alvaro
authors.initials: A
authors.lastname: Alonso
authors.forename: Manuela M
authors.initials: MM
authors.lastname: Pereira
authors.forename: Marisela
authors.initials: M
authors.lastname: Vélez
authors.forename: Antonio L
authors.initials: AL
authors.lastname: De Lacey
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D040761:Biomimetic Materials; D004566:Electrodes; D004579:Electron Transport; D042967:Electron Transport Complex I / Q000737:chemistry* / Q000302:isolation & purification; D006046:Gold / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D011522:Protons; D045245:Rhodothermus / Q000737:chemistry* / Q000201:enzymology; D013439:Sulfhydryl Reagents / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D008567:Membranes, Artificial; D011522:Protons; D013439:Sulfhydryl Reagents; D006046:Gold; D042967:Electron Transport Complex I