title: Diatom-derived polyunsaturated aldehydes activate cell death in human cancer cell lines but not normal cells.
abstract: Diatoms are an important class of unicellular algae that produce bioactive polyunsaturated aldehydes (PUAs) that induce abortions or malformations in the offspring of invertebrates exposed to them during gestation. Here we compare the effects of the PUAs 2-trans,4-trans-decadienal (DD), 2-trans,4-trans-octadienal (OD) and 2-trans,4-trans-heptadienal (HD) on the adenocarcinoma cell lines lung A549 and colon COLO 205, and the normal lung/brunch epithelial BEAS-2B cell line. Using the viability MTT/Trypan blue assays, we show that PUAs have a toxic effect on both A549 and COLO 205 tumor cells but not BEAS-2B normal cells. DD was the strongest of the three PUAs tested, at all time-intervals considered, but HD was as strong as DD after 48 h. OD was the least active of the three PUAs. The effect of the three PUAs was somewhat stronger for A549 cells. We therefore studied the death signaling pathway activated in A549 showing that cells treated with DD activated Tumor Necrosis Factor Receptor 1 (TNFR1) and Fas Associated Death Domain (FADD) leading to necroptosis via caspase-3 without activating the survival pathway Receptor-Interacting Protein (RIP). The TNFR1/FADD/caspase pathway was also observed with OD, but only after 48 h. This was the only PUA that activated RIP, consistent with the finding that OD causes less damage to the cell compared to DD and HD. In contrast, cells treated with HD activated the Fas/FADD/caspase pathway. This is the first report that PUAs activate an extrinsic apoptotic machinery in contrast to other anticancer drugs that promote an intrinsic death pathway, without affecting the viability of normal cells from the same tissue type. These findings have interesting implications also from the ecological viewpoint considering that HD is one of the most common PUAs produced by diatoms.
authors.affiliation: Stazione Zoologica Anton Dohrn, Naples, Italy.
authors.forename: Clementina
authors.initials: C
authors.lastname: Sansone
authors.affiliation: Stazione Zoologica Anton Dohrn, Naples, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Braca
authors.affiliation: Stazione Zoologica Anton Dohrn, Naples, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Ercolesi
authors.affiliation: Stazione Zoologica Anton Dohrn, Naples, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Romano
authors.affiliation: Stazione Zoologica Anton Dohrn, Naples, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Palumbo
authors.affiliation: Stazione Zoologica Anton Dohrn, Naples, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Casotti
authors.affiliation: Stazione Zoologica Anton Dohrn, Naples, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Francone
authors.affiliation: Stazione Zoologica Anton Dohrn, Naples, Italy.
authors.forename: Adrianna
authors.initials: A
authors.lastname: Ianora
mesh_terms: D000447:Aldehydes / Q000737:chemistry / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002460:Cell Line; D017377:Diatoms / Q000737:chemistry* / Q000378:metabolism; D053200:Fas-Associated Death Domain Protein / Q000378:metabolism; D006801:Humans; D053422:Receptor-Interacting Protein Serine-Threonine Kinases / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000447:Aldehydes; C505533:FADD protein, human; D053200:Fas-Associated Death Domain Protein; D047888:Receptors, Tumor Necrosis Factor, Type I; C057349:2,4-decadienal; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; D053148:Caspase 3