title: Targeting oxidative stress attenuates trinitrobenzene sulphonic acid induced inflammatory bowel disease like symptoms in rats: role of quercetin.
abstract: OBJECTIVE
  This study was aimed to investigate the beneficial effects of quercetin (QCT) against trinitrobenzene sulfonic acid (TNBS) induced clinical, morphological, and biochemical alterations in rats.
  
  
  METHODS
  Colitis in rats was induced by administration of TNBS (25 mg dissolved in 0.25 ml of 30% ethanol) 8 cm into the rectum of the rat using a catheter. The animals were divided into six experimental groups (n = 6); naive (saline only without TNBS administration), control (saline + TNBS), standard (sulfasalazine 25 mg/kg + TNBS), QCT (25) (QCT 25 mg/kg + TNBS), QCT (50) (QCT 50 mg/kg + TNBS), QCT (100) (QCT 100 mg/kg + TNBS). Sulfasalazine (25 mg/kg) and QCT (25, 50 and 100 mg/kg) were administered per oral for 11 days and the colonic damage was evaluated in terms of macroscopical (body weight, stool consistency, rectal bleeding, and ulcer index) and biochemical parameters (myeloperoxidase activity, lipid peroxidation, nitrite, and glutathione).
  
  
  RESULTS
  Treatment with QCT (50, 100 mg/kg) for 10 days following TNBS administration significantly attenuated the clinical, morphological, and biochemical alterations induced by TNBS, whereas it was found to be not effective at its lower dose (25 mg/kg) throughout the experimental protocol.
  
  
  CONCLUSIONS
  QCT attenuates the clinical, morphological and biochemical alterations induced by TNBS possibly via its antioxidant mechanism.
authors.affiliation: University College of Pharmaceutical Sciences, Kakatiya University, Department of Pharmacology, Warangal, Andhra Pradesh, India ; Vaagdevi College of Pharmacy, Warangal, Andhra Pradesh, India.
authors.forename: Dilip
authors.initials: D
authors.lastname: Dodda
authors.affiliation: Departments of Pharmacology, NMT Gujarati College of Pharmacy, Indore, Madhya Pradesh, India.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Chhajed
authors.affiliation: Roland Institute of Pharmaceutical Sciences, Khodasingi, Berhampur, Odisha, India ; Pharmacology Division, University Institute of Pharmaceutical Sciences, UGC Center of Advanced Studies, Panjab University, Chandigarh, India.
authors.forename: Jitendriya
authors.initials: J
authors.lastname: Mishra
authors.affiliation: Roland Institute of Pharmaceutical Sciences, Khodasingi, Berhampur, Odisha, India.
authors.forename: Monalisa
authors.initials: M
authors.lastname: Padhy
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D000975:Antioxidants; D009566:Nitrates; D009573:Nitrites; D014302:Trinitrobenzenesulfonic Acid; D011794:Quercetin; D005978:Glutathione
keywords: Inflammatory bowel disease; myeloperoxidase; oxidative stress; ulcer index