title: [Protective effects of WR2721 on early bone marrow hematopoietic function in mice exposed to 6.5 Gy of (60)Co γ-rays].
abstract: The aim of this study was to investigate the effect of WR2721(amifostine) against bone marrow hematopoietic damage of mice exposed to 6.5 Gy of (60)Co-γ ray. A total of 60 C57/BL6J mice was divided into 3 groups:normal group (mice were injected with physiological salt solution), irradiation group (mice were injected with physiologic salt solution before irradiation) and WR2721 group (mice were injected with WR2721 before irradiation). The WBC, neutrophil (Neut), Plt and RBC levels in peripheral blood of 3 group mice were counted within 60 days after irradiation; the bone marrow nuclear cells (BMNC) were counted at 2 and 24 hours after irradiation; the hematopoietic stem/progenitor cell (LK/LSK) level and colony formation capability were detected by flow cytometry at 2 and 24 hours after irradiation. The results indicated that the counts of WBC and neut at 4 and 18 days, Plt at 7-18 days and RBC at 10-30 day after irradiation in WR2721 group were higher than those in irradiation group (P < 0.05); the BMNC, LSK and LK levels obviously increased at 24 hours after irradiation (P < 0.05), the CFU-GEMM, CFU-GM, CFU-MK BFU-E and CFU-E all significantly increased at 2 and 24 hours after irradiation (P < 0.01), as compared with irradiation group. It is concluded that WR2721 can effectively alleviate early hematopoietic damage and promote the fast recovery of peripheral blood cells in mice exposed to γ-ray, suggesting that the WR2721 has significant radioprotective effect on hematopoietic system.
authors.affiliation: Anhui Medical University, Hefei 230032, Anhui Province, China; Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Zi-Liang
authors.initials: ZL
authors.lastname: Deng
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Liu-Zhen
authors.initials: LZ
authors.lastname: Zhang
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cong
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Zu-Ying
authors.initials: ZY
authors.lastname: Yu
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Shan
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Wang
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Xing
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China. E-mail:Congyw01@163.com.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Cong
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China. E-mail: lql20093353@163.com.
authors.forename: Qing-Liang
authors.initials: QL
authors.lastname: Luo
mesh_terms: D004999:Amifostine / Q000494:pharmacology*; D000818:Animals; D001772:Blood Cell Count; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014916:Whole-Body Irradiation
chemical_list: D011837:Radiation-Protective Agents; D004999:Amifostine