title: Dietary fat alters the expression of cortistatin and ghrelin systems in the PBMCs of elderly subjects: putative implications in the postprandial inflammatory response.
abstract: METHODS
  Dietary fat influences systemic inflammatory status, which determines the progression of age-associated diseases. Since somatostatin (SST), cortistatin (CORT), and ghrelin systems modulate inflammatory response, we aim to comprehensively characterize the presence and regulation of the components of these systems in the peripheral blood mononuclear cells (PMBCs), a subset of white blood cells placed at the crossroad between diet and inflammation, in response to diets with different fat composition, and during the postprandial phase in elderly subjects.
  
  
  RESULTS
  The applied nutrigenomic, inflammation-related PBMC-based approach revealed that the majority of components of SST/CORT and ghrelin systems are present in the human PBMCs. Particularly, CORT, SST/CORT receptors (sst2, sst3, sst5, and sst5TMD4), ghrelin, its acylating enzyme (GOAT), In1-ghrelin variant, and GHSR1b were detected in PBMCs. Their expression was altered in the long-term by diet composition, and in the short-term, during the postprandial phase. Of particular relevance is the postprandial elevation of CORT, sst2, and sst5 expression in PBMCs of subjects under n-3 PUFAs-enriched diet.
  
  
  CONCLUSIONS
  Our results suggest a potential relevant role of CORT/ssts and ghrelin systems in regulating PBMCs response to nutrient intake, which could help to explain the positive effects of n-3 PUFAs-enriched diets in reducing the inflammatory response.
authors.affiliation: Department of Cell Biology, Physiology, and Immunology, University of Cordoba, Reina Sofia University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), CIBER Fisiopatología de la Obesidad y Nutrición (CIBERObn), Córdoba, Spain; Lipid and Atherosclerosis Research Unit, Reina Sofia University Hospital, University of Cordoba, IMIBIC and CIBERObn, Córdoba, Spain.
authors.forename: Manuel D
authors.initials: MD
authors.lastname: Gahete
authors.forename: Raúl M
authors.initials: RM
authors.lastname: Luque
authors.forename: Elena M
authors.initials: EM
authors.lastname: Yubero-Serrano
authors.forename: Cristina
authors.initials: C
authors.lastname: Cruz-Teno
authors.forename: Alejandro
authors.initials: A
authors.lastname: Ibañez-Costa
authors.forename: Javier
authors.initials: J
authors.lastname: Delgado-Lista
authors.forename: Francisco
authors.initials: F
authors.lastname: Gracia-Navarro
authors.forename: Francisco
authors.initials: F
authors.lastname: Perez-Jimenez
authors.forename: Justo P
authors.initials: JP
authors.lastname: Castaño
authors.forename: José
authors.initials: J
authors.lastname: Lopez-Miranda
mesh_terms: D000368:Aged; D038441:Diet, Mediterranean; D066273:Diet, Western; D004041:Dietary Fats / Q000494:pharmacology*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology; D005260:Female; D054439:Ghrelin / Q000097:blood* / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009479:Neuropeptides / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D054647:Nutrigenomics / Q000379:methods; D019518:Postprandial Period / Q000187:drug effects*; D017481:Receptors, Somatostatin / Q000235:genetics; D013004:Somatostatin / Q000235:genetics
chemical_list: D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D054439:Ghrelin; D009479:Neuropeptides; D017481:Receptors, Somatostatin; C099323:cortistatin; C098681:somatostatin receptor subtype-4; D013004:Somatostatin; C094573:somatostatin receptor 5
keywords: Cortistatin; Ghrelin; PBMC; PUFA; Postprandial