title: Metagenomic small molecule discovery methods.
abstract: Metagenomic approaches to natural product discovery provide the means to harvest bioactive small molecules synthesized by environmental bacteria without the requirement of first culturing these organisms. Advances in sequencing technologies and general metagenomic methods are beginning to provide the tools necessary to unlock the unexplored biosynthetic potential encoded by the genomes of uncultured environmental bacteria. Here, we highlight recent advances in sequence-based and functional-based metagenomic approaches that promise to facilitate antibiotic discovery from diverse environmental microbiomes.
authors.affiliation: Laboratory of Genetically Encoded Small Molecules, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, United States.
authors.forename: Zachary
authors.initials: Z
authors.lastname: Charlop-Powers
authors.affiliation: Laboratory of Genetically Encoded Small Molecules, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, United States.
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Milshteyn
authors.affiliation: Laboratory of Genetically Encoded Small Molecules, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, United States. Electronic address: sbrady@rockefeller.edu.
authors.forename: Sean F
authors.initials: SF
authors.lastname: Brady
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D056186:Metagenomics; D054852:Small Molecule Libraries
chemical_list: D000900:Anti-Bacterial Agents; D054852:Small Molecule Libraries