title: Stereoselective metabolism, distribution, and bioaccumulation brof triadimefon and triadimenol in lizards.
abstract: In this research, Chinese lizards (Eremias argus) were chosen as laboratory animal to evaluate the stereoselectivity in the processes of metabolism, distribution, and bioaccumulation of triadimefon. A validated chiral high-performance liquid chromatography coupled with triple quadruple mass spectrometry (HPLC-MS/MS) method was developed for determining enantiomers' residues of parent compound triadimefon and its metabolite triadimenol in lizard blood and tissues. Pharmacokinetic results of single-does exposure suggested that S-(+)-triadimefon was metabolized easier than R-(-)-triadimefon, and RR-(+)-triadimenol was the main metabolic product of triadimefon. During the continuous exposure of two dose (40mg/kg(bw)·d and 200mg/kg(bw)·d), enantiomers of triadimefon and triadimenol were detected in all body compartments, with the highest triadimefon concentrations in brain. However, the triadimenol concentrations were not significantly different among the compartments. The concentrations of RS-(+)-triadimenol were negative correlated with concentrations of RR-(+)-triadimenol both in blood (r=-0.775, p=0.024) and liver (r=-0.834, p=0.02) in 200mg/kg(bw)·d group, which indicates that chiral conversion between enantiomers of triadimenol might exist in the metabolic process of triadimefon. In all the processes, the enantiomer fractions (EFs) of R-(-)-triadimefon and RR-(+)-triadimenol were significantly different from their natural ratios, 0.5 and 0.1, respectively, which proved that metabolism, bioaccumulation, and distribution of triadimefon and triadimenol in lizards were enantioselective. These results help enrich and supplement the knowledge of the stereoselective behaviour of triadimefon and triadimenol in reptile.
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing 100085, PR China.
authors.forename: Yinghuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing 100085, PR China.
authors.forename: Dingyi
authors.initials: D
authors.lastname: Yu
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing 100085, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing 100085, PR China.
authors.forename: Baoyuan
authors.initials: B
authors.lastname: Guo
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing 100085, PR China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing 100085, PR China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Li
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing 100085, PR China. Electronic address: huiliwang@rcees.ac.cn.
authors.forename: Huili
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005659:Fungicides, Industrial / Q000378:metabolism* / Q000493:pharmacokinetics / Q000633:toxicity; D007700:Kinetics; D008116:Lizards / Q000097:blood*; D008297:Male; D011897:Random Allocation; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution; D018675:Toxicity Tests; D014230:Triazoles / Q000378:metabolism* / Q000493:pharmacokinetics / Q000633:toxicity
chemical_list: D005659:Fungicides, Industrial; D014230:Triazoles; C032910:triadimefon
keywords: Eremias argus; Stereoselectivity; Triadimefon; Triadimenol