title: Evaluating experimental cerebral malaria using oxidative stress indicator OKD48 mice.
abstract: Cerebral malaria is a fatal complication of malaria. Conventional methods for evaluating experimental cerebral malaria have several drawbacks. Therefore, we aimed to develop an easy-to-use method for evaluating experimental cerebral malaria using OKD48 (Keap1-dependent Oxidative stress Detector, No-48-luciferase) mice to evaluate oxidative stress. OKD48 mice infected with Plasmodium berghei ANKA strain (PbA) suffered from experimental cerebral malaria and oxidative stress was successfully detected in the brains of living OKD48 mice developing experimental cerebral malaria. Oxidative stress in the brain was dependent on the development of experimental cerebral malaria, as prevention of experimental cerebral malaria did not elicit oxidative stress. We provide a novel evaluation method for experimental cerebral malaria using oxidative stress indicator OKD48 mice.
authors.affiliation: Department of Parasitology, Graduate School of Medicine, Gunma University, Gunma 371-8511, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Imai
authors.affiliation: Iwawaki Laboratory, Advanced Scientific Research Leaders Development Unit, Gunma University, Gunma 371-8511, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Iwawaki
authors.affiliation: Iwawaki Laboratory, Advanced Scientific Research Leaders Development Unit, Gunma University, Gunma 371-8511, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Akai
authors.affiliation: Department of Parasitology, Graduate School of Medicine, Gunma University, Gunma 371-8511, Japan.
authors.forename: Kazutomo
authors.initials: K
authors.lastname: Suzue
authors.affiliation: Department of Parasitology, Graduate School of Medicine, Gunma University, Gunma 371-8511, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirai
authors.affiliation: Department of Parasitology, Graduate School of Medicine, Gunma University, Gunma 371-8511, Japan; Center for Medical Education, Faculty of Medicine, Gunma University, Gunma 371-8511, Japan.
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Taniguchi
authors.affiliation: Department of Parasitology, Graduate School of Medicine, Gunma University, Gunma 371-8511, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Okada
authors.affiliation: Department of Parasitology, Graduate School of Medicine, Gunma University, Gunma 371-8511, Japan. Electronic address: hisa@gunma-u.ac.jp.
authors.forename: Hajime
authors.initials: H
authors.lastname: Hisaeda
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000627:therapeutic use; D001921:Brain / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D008163:Luminescent Measurements; D016779:Malaria, Cerebral / Q000378:metabolism / Q000469:parasitology*; D051379:Mice; D008815:Mice, Inbred Strains; D018384:Oxidative Stress / Q000502:physiology*; D010962:Plasmodium berghei; D010967:Plasmodium yoelii; D011739:Pyrimethamine / Q000627:therapeutic use
chemical_list: D000962:Antimalarials; D011739:Pyrimethamine
keywords: Experimental cerebral malaria; In vivo imaging; Malaria; Oxidative stress