title: Effect of high-fat diet upon inflammatory markers and aortic stiffening in mice.
abstract: Changes in lifestyle such as increase in high-fat food consumption are an important cause for vascular diseases. The present study aimed to investigate the involvement of ACE and TGF- β in the aorta stiffness induced by high-fat diet. C57BL/6 male mice were divided in two groups according to their diet for 8 weeks: standard diet (ST) and high-fat diet (HF). At the end of the protocol, body weight gain, adipose tissue content, serum lipids and glucose levels, and aorta morphometric and biochemical measurements were performed. Analysis of collagen fibers by picrosirius staining of aorta slices showed that HF diet promoted increase of thin (55%) and thick (100%) collagen fibers deposition and concomitant disorganization of these fibers orientations in the aorta vascular wall (50%). To unravel the mechanism involved, myeloperoxidase (MPO) and angiotensin I converting enzyme (ACE) were evaluated by protein expression and enzyme activity. HF diet increased MPO (90%) and ACE (28%) activities, as well as protein expression of ACE. TGF-β was also increased in aorta tissue of HF diet mice after 8 weeks. Altogether, we have observed that the HF diet-induced aortic stiffening may be associated with increased oxidative stress damage and activation of the RAS in vascular tissue.
authors.affiliation: Institute of Biomedical Sciences, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil.
authors.forename: Andre Bento Chaves
authors.initials: AB
authors.lastname: Santana
authors.affiliation: Institute of Biomedical Sciences, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil.
authors.forename: Thais Cristina
authors.initials: TC
authors.lastname: de Souza Oliveira
authors.affiliation: Institute of Biomedical Sciences, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil.
authors.forename: Barbara Lobo
authors.initials: BL
authors.lastname: Bianconi
authors.affiliation: Heart Institute, University of Sao Paulo Medical School, 05403-900 Sao Paulo, SP, Brazil.
authors.forename: Valerio Garrone
authors.initials: VG
authors.lastname: Barauna
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil.
authors.forename: Ed Wilson Cavalcante Oliveira
authors.initials: EW
authors.lastname: Santos
authors.affiliation: Institute of Biomedical Sciences, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil.
authors.forename: Tatiana P
authors.initials: TP
authors.lastname: Alves
authors.affiliation: Institute of Biomedical Sciences, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil.
authors.forename: Juliane Cristina S
authors.initials: JC
authors.lastname: Silva
authors.affiliation: Health, Biology and Science Center, Mackenzie University, 01302-907 Sao Paulo, SP, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Fiorino
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil.
authors.forename: Primavera
authors.initials: P
authors.lastname: Borelli
authors.affiliation: Heart Institute, University of Sao Paulo Medical School, 05403-900 Sao Paulo, SP, Brazil.
authors.forename: Maria Claudia Costa
authors.initials: MC
authors.lastname: Irigoyen
authors.affiliation: Heart Institute, University of Sao Paulo Medical School, 05403-900 Sao Paulo, SP, Brazil.
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Krieger
authors.affiliation: Institute of Biomedical Sciences, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil.
authors.forename: Silvia
authors.identifier: 0000-0002-7937-8473
authors.initials: S
authors.lastname: Lacchini
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D059289:Vascular Stiffness / Q000187:drug effects*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D015415:Biomarkers; D016212:Transforming Growth Factor beta; D007703:Peptidyl-Dipeptidase A