title: Roles of CXCL5 on migration and invasion of liver cancer cells.
abstract: Inflammatory factors play a vital role in the progression of liver cancer, although exact factors and related mechanisms still remain unclear. The present study aimed at screening inflammatory factors related to liver cancer metastasis and investigating the potential mechanism by which cancer cells are recruited. We screened and validated inflammatory factors by microarray and RT-PCR. Small interfering RNA (siRNA) and recombinant protein were used to assess CXCL5 effects on the movement of liver cancer cells (LCCs). Our screening microarray demonstrated over-expression of CXCL5 in LCCs with high metastatic potentials. CXCL5 increased LCCs migration and invasion, probably through autocrine and paracrine mechanisms. CXCL5-CXCR2 and ERK1/2 pathways could play critical roles in the regulation of LCCs migration. Our data indicates that LCCs per se may act as the producer and receptor of CXCL5 responsible for liver cancer migration and invasion.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Xu
authors.forename: Peixin
authors.initials: P
authors.lastname: Huang
authors.forename: Biwei
authors.initials: B
authors.lastname: Yang
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University; Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai 200032, China. xiangdong.wang@clintransmed.org.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Wang
authors.forename: Jinglin
authors.initials: J
authors.lastname: Xia
mesh_terms: D015153:Blotting, Western; D054365:Chemokine CXCL5 / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology* / Q000503:physiopathology; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D016133:Polymerase Chain Reaction
chemical_list: C486263:CXCL5 protein, human; D054365:Chemokine CXCL5