title: A novel monopartite dsRNA virus isolated from the phytopathogenic fungus Ustilaginoidea virens and ancestrally related to a mitochondria-associated dsRNA in the green alga Bryopsis.
abstract: In this study, we describe a novel mycovirus isolated from Ustilaginoidea virens, which was designated Ustilaginoidea virens nonsegmented virus 1 (UvNV-1). The sequence analysis revealed that UvNV-1 has two open reading frames (ORFs). ORF1 encodes an unknown protein, which is similar to the hypothetical protein BN7_5177 of Wickerhamomyces ciferrii. ORF2 encodes a putative RNA-dependent RNA polymerase (RdRp), which is most closely related to Bryopsis mitochondria-associated dsRNA (BDRM) and is likely expressed by a +1 ribosomal frameshift within the sequence CCC_UUU_CGA. The phylogenetic analysis of the RdRp of UvNV-1 showed that UvNV-1 represents a new virus taxon of mycoviruses with a partitivirus-like lineage that is classified into the family of picorna-like viruses. Based on northern hybridization, UvNV-1 was found to be common to U. virens from different geographic locations in China. The biological comparison of virus-free and infected fungal strains revealed that UvNV-1 is likely to be cryptic to its host.
authors.affiliation: Department of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China; The Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China; The Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China.
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China; The Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China. Electronic address: dwb@mail.hzau.edu.cn.
authors.forename: Wubei
authors.initials: W
authors.lastname: Dong
mesh_terms: D002681:China; D000460:Chlorophyta / Q000235:genetics; D016000:Cluster Analysis; D016679:Genome, Viral; D006999:Hypocreales / Q000821:virology*; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D004718:Saccharomycetales / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral
keywords: BDRM; Frameshift translation; Horizontal gene transfer; Mycovirus; Picorna-like viruses; Ustilaginoidea virens; dsRNA