title: Strontium promotes cementoblasts differentiation through inhibiting sclerostin expression in vitro.
abstract: Cementogenesis, performed by cementoblasts, is important for the repair of root resorption caused by orthodontic treatment. Based on recent studies, strontium has been applied for osteoporosis treatment due to its positive effect on osteoblasts. Although promising, the effect of strontium on cementoblasts is still unclear. So the aim of this research was to clarify and investigate the effect of strontium on cementogenesis via employing cementoblasts as model. A series of experiments including MTT, alkaline phosphatase activity, gene analysis, alizarin red staining, and western blot were carried out to evaluate the proliferation and differentiation of cementoblasts. In addition, expression of sclerostin was checked to analyze the possible mechanism. Our results show that strontium inhibits the proliferation of cementoblasts with a dose dependent manner; however, it can promote the differentiation of cementoblasts via downregulating sclerostin expression. Taking together, strontium may facilitate cementogenesis and benefit the treatment of root resorption at a low dose.
authors.affiliation: Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun, Jilin 130021, China.
authors.forename: Xingfu
authors.initials: X
authors.lastname: Bao
authors.affiliation: Institute of Virology and AIDS Research, First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun, Jilin 130021, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun, Jilin 130021, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun, Jilin 130021, China.
authors.forename: Jindan
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun, Jilin 130021, China.
authors.forename: Min
authors.identifier: 0000-0001-9783-7859
authors.initials: M
authors.lastname: Hu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003739:Dental Cementum / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D007477:Ions; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D013194:Staining and Labeling; D013324:Strontium / Q000494:pharmacology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000880:Anthraquinones; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D007477:Ions; C486030:Sost protein, mouse; C010078:alizarin; D000469:Alkaline Phosphatase; D013324:Strontium