title: Netrin-1 promoted pancreatic cancer cell proliferation by upregulation of Mdm2.
abstract: Netrin-1 displays proto-oncogenic activity in several cancers, which is thought to result from the ability of netrin-1 secretions to stimulate survival when bound to associated receptors. The objective of this study was to determine the role of netrin-1 in pancreatic cancer cell proliferation in vitro. Our results revealed that netrin-1 overexpression promoted while its silence inhibited two pancreatic cancer cell lines. At the molecular level, we found that netrin-1 promoted cell proliferation by upregulation of murine double minute 2 (Mdm2). As a result, p53 protein contents were reduced in cells overexpressing netrin-1. Therefore, our data suggests the presence of a previously unknown network in the proliferation and progression of pancreatic cancer cells.
authors.affiliation: Department of Oncology, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200093, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Huang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Hua
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
authors.forename: Dai-He
authors.initials: DH
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Ding
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D009414:Nerve Growth Factors / Q000378:metabolism*; D000075388:Netrin-1; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism*; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C000617351:NTN1 protein, human; D009414:Nerve Growth Factors; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2