title: Acetylcholinesterase inhibitory activity of Chinese sufu (fermented tofu) ethanol-extract.
abstract: Acetylcholinesterase (AChE) inhibitory activities of commercial sufu and self-produced sufu were investigated in this experiment. The anti-AChE activities of commercial sufu samples of 15 brands, sourcing from various parts of China, and self-produced sufu, fermented with Actinomucor elegans 3.118, were measured. The results indicated that ethanol extract of Chinese sufu exhibited significant inhibitory activity against AChE in vitro. The inhibitory activity of No. 5 sufu was the strongest (IC₅₀, 0.191 mg/ml), while the pre-fermented sufu showed the highest inhibitory activity during sufu manufacturing. In addition, soybean extracts and potato extracts were used to culture A. elegans 3.118 in order to estimate which culture was preferable for the production of these AChE inhibitors. The soybean extracts, after fermentation by A. elegans 3.118, showed higher anti-AChE activity than did the potato extracts. The IC₅₀ of the soybean extracts was 1.29 μg/ml.
authors.affiliation: Logistics School, Beijing Wuzi University, 1 Fuhe da jie, Tongzhou, Beijing 101149, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Food Science & Nutritional Engineering, China Agricultural University, 17 Qinghua Dong, Haidian, Beijing 100083, China.
authors.forename: Ming-hai
authors.initials: MH
authors.lastname: Quan
authors.affiliation: College of Food Science & Nutritional Engineering, China Agricultural University, 17 Qinghua Dong, Haidian, Beijing 100083, China.
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Cheng
authors.affiliation: Mengniu Diary (Beijing) Co., Ltd., 1 Food Industry Park, Beijing 101107, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Food Science & Nutritional Engineering, China Agricultural University, 17 Qinghua Dong, Haidian, Beijing 100083, China.
authors.forename: Li-te
authors.initials: LT
authors.lastname: Li
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism; D002681:China; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry*; D005285:Fermentation; D010936:Plant Extracts / Q000494:pharmacology*; D045730:Soy Foods / Q000382:microbiology*
chemical_list: D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D000431:Ethanol; D000110:Acetylcholinesterase