title: Prevalence and genetic profiles of Escherichia coli from mangroves and mangrove associated foods off Goa, India.
abstract: A total of 120 samples comprising of water (45), sediment (45) and mangrove originated food (30) collected from mangrove ecosystems of Goa were screened for Escherichia coli employing ISO-16654 method. Seventy-one (59.16%) samples were positive for E. coli. The E. coli isolates were further characterized by serotyping, virulence gene profiling and pulsed field gel electrophoresis (PFGE). Water and sediment samples were analyzed for physico-chemical parameters. The serotypes reported were O1, O10, O13, O17, O36, O41, O50, O68, O105, O116, O141, O148, O159, O162 and rough types while, 23 strains could not be typed. The stx1 and stx2 genes were detected in 33(46.47%) and 16(22.53%) isolates, respectively. The XbaI restriction digestion patterns of the stx positive strains were diverse. Interestingly, few strains isolated from diarrheal patients and from water, sediment and food from mangrove sources were genetically similar. The study showed that the mangrove ecosystem could be a potential reservoir for pathogenic E. coli.
authors.affiliation: ICAR Research Complex for Goa, Old Goa 403 402, India.
authors.forename: Krupali V
authors.initials: KV
authors.lastname: Poharkar
authors.affiliation: Department of Biotechnology, Goa University, Taleigaon Plateau, Goa 403 206, India.
authors.forename: Savita
authors.initials: S
authors.lastname: Kerkar
authors.affiliation: ICAR Research Complex for Goa, Old Goa 403 402, India.
authors.forename: Swapnil P
authors.initials: SP
authors.lastname: Doijad
authors.affiliation: ICAR Research Complex for Goa, Old Goa 403 402, India. Electronic address: barbuddhesb@yahoo.com.
authors.forename: S B
authors.initials: SB
authors.lastname: Barbuddhe
mesh_terms: D032407:Avicennia; D003967:Diarrhea / Q000382:microbiology; D017753:Ecosystem; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000235:genetics*; D063366:Estuaries; D005516:Food Microbiology; D020869:Gene Expression Profiling; D005843:Geography; D019015:Geologic Sediments / Q000382:microbiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007194:India; D012703:Serotyping; D013696:Temperature; D014871:Water Microbiology
keywords: Escherichia coli; Mangrove; PFGE; Serotypes