title: PTEN mutations and activation of the PI3K/Akt/mTOR signaling pathway in papillary tumors of the pineal region.
abstract: Papillary tumors of the pineal region (PTPR) are recognized as a distinct entity in the World Health Organization classification of CNS tumors. Papillary tumors of the pineal region frequently show loss of chromosome 10, but no studies have investigated possible target genes on this chromosome. Chromosome 10 harbors the PTEN (phosphatase and tensin homolog) gene, the inactivation of which, by mutation or epigenetic silencing, has been observed in different brain tumors, including high-grade gliomas. In this study, we investigated copy number changes by molecular inversion probe (MIP) analysis and the mutational status of PTEN in 13 PTPR by direct sequencing. MIP analysis of 5 PTPR showed chromosome 10 loss in all cases. In addition, there were losses of chromosomes 3, 14, 22, and X, and gains of whole chromosomes 8, 9, and 12 in more than 1 case. One case had a homozygous PTEN deletion; and 2 point mutations in exon 7 of PTEN (G251D and Q261stop) were found. Immunohistochemistry revealed decrease or loss of the PTEN protein and increased expression of p-Akt and p-S6. These results indicated that PTEN mutations and activation of the PI3K/Akt/mTOR signaling pathway may play a role in the biology of PTPR. This evidence may lead to the possible use of PI3K/Akt/mTOR inhibitors in therapy for patients with PTPR.
authors.affiliation: From the Institute of Neuropathology, University of Bonn Medical Center, Bonn, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Goschzik
authors.forename: Marco
authors.initials: M
authors.lastname: Gessi
authors.forename: Dorota
authors.initials: D
authors.lastname: Denkhaus
authors.forename: Torsten
authors.initials: T
authors.lastname: Pietsch
mesh_terms: D000293:Adolescent; D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D007633:Keratins / Q000378:metabolism; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008875:Middle Aged; D018396:Mucin-1 / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D018302:Neoplasms, Neuroepithelial / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D010870:Pineal Gland / Q000473:pathology*; D010871:Pinealoma / Q000235:genetics*; D009418:S100 Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D055815:Young Adult
chemical_list: D008869:Microtubule-Associated Proteins; D018396:Mucin-1; D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D007633:Keratins; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human