title: [Mechanism of antitumor effect of ursolic acid on T (8;21) leukemia cell kasumi-1].
abstract: This study was purposed to investigate the anti-tumor effect of ursolic acid (UA) on t(8;21) leukemia cell line kasumi-1 and its possible mechanisms. The kasumi-1 cells were treated with UA at different concentration for different duration of time. The growth inhibition of kasumi-1 treated with UA was detected by using CCK-8 test, and the morphological changes of kasumi-1 cells were observed by Wright's staining. Furthermore, the apoptosis rate of kasumi-1 was examined by flow cytometry. Lastly, the expression of AML1-ETO, KIT, MYC, CCND1, BCL-2, P53, BAX, MDM2 and protein were detected by using real-time quantitative PCR and Western blot respectively. The results showed that the UA obviously inhibited the growth of kasumi-1 cells in dose- and time-dependent manners. The apoptotic morphological changes of cells were presented when kasumi-1 cells were treated with UA for 48 hours. The apoptotic rate of kasumi-1 cells increased in a dose- and time-dependent ways, and the mRNA levels of AML1-ETO, KIT, MYC, CCND1, BCL2, MDM2 decreased in kasumi-1 cells treated with UA, as well as the protein levels. Meanwhile, UA up-regulated the mRNA and protein levels of P53 in the same manner. It is concluded that UA can exert its anti-tumor effect by inhibiting the proliferation and inducing the apoptosis of kasumi-1 cells in a dose-and time-dependent manners, that may provide the clues for a new targeting therapy to t(8;21) leukemia.
authors.affiliation: Department of Hematology, China-Japanese Friendship Hospital, Beijing 100029, China.
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: Department of Hematology, China-Japanese Friendship Hospital, Beijing 100029, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Clinical Laboratorial Examination, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Hao-Qing
authors.initials: HQ
authors.lastname: Duan
authors.affiliation: Department of Hematology, China-Japanese Friendship Hospital, Beijing 100029, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Hematology, China-Japanese Friendship Hospital, Beijing 100029, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Hematology, China-Japanese Friendship Hospital, Beijing 100029, China. E-mail:dr_myg@yahoo.com.cn.
authors.forename: Yi-Gai
authors.initials: YG
authors.lastname: Ma
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D007938:Leukemia / Q000235:genetics; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D014178:Translocation, Genetic; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D015514:Oncogene Proteins, Fusion; D014315:Triterpenes; D000097245:Ursolic Acid