title: Expression features of CXCR5 and its ligand, CXCL13 associated with poor prognosis of advanced colorectal cancer.
abstract: OBJECTIVE
  CXCL13 plays a unique role in the trafficking and homing of B1 cells associated with its cognate receptor, CXCR5. The CXCR5-CXCL13 axis has been previously demonstrated to be a poor prognosis factor in malignancies. However, the clinical significance of the CXCR5-CXCL13 expression in colorectal cancer carcinoma (CRC) remains unclear. The aim of this study was to investigate the CXCR5-CXCL13 expression in CRC and determine its correlation with the progression and prognosis of the tumor.
  
  
  METHODS
  A total of 144 paraffin-embedded specimens with advanced colon cancer were assessed for CXCR5 and CXCL13 by immunohistochemistry. Patients' long-term survival was also monitored. There were significant differences in lymph node metastasis (p = 0.0066), neural invasion (p = 0.0061) and neural invasion (p = 0.0001) between high and low expression of CXCR5.
  
  
  RESULTS
  There were significant differences in distant metastasis (p = 0.0261), TNM stage (p = 0.0409), differentiation (p < 0.0001) and neural invasion of the CXCL13. Both CXCR5 and CXCL13 was associated with poor correlation with the overall survival (OS) and relapse-free survival (RFS).
  
  
  CONCLUSIONS
  Our data suggest that the CXCR5 and CXCL13 may play a crucial role in the development, metastasis and relapse of advanced colon cancer. They can be used as prognostic markers of colon cancer in clinical practice.
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, China. haorongwusdfey@163.com.
authors.forename: X-W
authors.initials: XW
authors.lastname: Qi
authors.forename: S-H
authors.initials: SH
authors.lastname: Xia
authors.forename: Y
authors.initials: Y
authors.lastname: Yin
authors.forename: L-F
authors.initials: LF
authors.lastname: Jin
authors.forename: Y
authors.initials: Y
authors.lastname: Pu
authors.forename: D
authors.initials: D
authors.lastname: Hua
authors.forename: H-R
authors.initials: HR
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D054382:Chemokine CXCL13 / Q000378:metabolism*; D003106:Colon / Q000473:pathology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008024:Ligands; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D054380:Receptors, CXCR5 / Q000378:metabolism*; D012007:Rectum / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C110663:CXCL13 protein, human; C095085:CXCR5 protein, human; D054382:Chemokine CXCL13; D008024:Ligands; D054380:Receptors, CXCR5