title: TRICHOME BIREFRINGENCE-LIKE27 affects aluminum sensitivity by modulating the O-acetylation of xyloglucan and aluminum-binding capacity in Arabidopsis.
abstract: Xyloglucan (XyG) has been reported to contribute to the aluminum (Al)-binding capacity of the cell wall in Arabidopsis (Arabidopsis thaliana). However, the influence of O-acetylation of XyG, accomplished by the putative O-acetyltransferase TRICHOME BIREFRINGENCE-LIKE27 (TBL27 [AXY4]), on its Al-binding capacity is not known. In this study, we found that the two corresponding TBL27 mutants, axy4-1 and axy4-3, were more Al sensitive than wild-type Columbia-0 plants. TBL27 was expressed in roots as well as in leaves, stems, flowers, and siliques. Upon Al treatment, even within 30 min, TBL27 transcript accumulation was strongly down-regulated. The mutants axy4-1 and axy4-3 accumulated significantly more Al in the root and wall, which could not be correlated with pectin content or pectin methylesterase activity, as no difference in the mutants was observed compared with the wild type when exposed to Al stress. The increased Al accumulation in the wall of the mutants was found to be in the hemicellulose fraction. While the total sugar content of the hemicellulose fraction did not change, the O-acetylation level of XyG was reduced by Al treatment. Taken together, we conclude that modulation of the O-acetylation level of XyG influences the Al sensitivity in Arabidopsis by affecting the Al-binding capacity in the hemicellulose.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences (X.F.Z., Y.S, J.X.W., Y.L., Z.W.W., S.J.Z.), and State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (B.C.Z., Y.H.Z), Chinese Academy of Sciences, Beijing 100101, China;Department of Plant and Microbial Biology University of California, Berkeley, California, 94720 (N.M.);Department of Plant Physiology and Nutrition, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China (Y.Z.S.).
authors.forename: Xiao Fang
authors.initials: XF
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences (X.F.Z., Y.S, J.X.W., Y.L., Z.W.W., S.J.Z.), and State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (B.C.Z., Y.H.Z), Chinese Academy of Sciences, Beijing 100101, China;Department of Plant and Microbial Biology University of California, Berkeley, California, 94720 (N.M.);Department of Plant Physiology and Nutrition, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China (Y.Z.S.).
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences (X.F.Z., Y.S, J.X.W., Y.L., Z.W.W., S.J.Z.), and State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (B.C.Z., Y.H.Z), Chinese Academy of Sciences, Beijing 100101, China;Department of Plant and Microbial Biology University of California, Berkeley, California, 94720 (N.M.);Department of Plant Physiology and Nutrition, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China (Y.Z.S.).
authors.forename: Bao Cai
authors.initials: BC
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences (X.F.Z., Y.S, J.X.W., Y.L., Z.W.W., S.J.Z.), and State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (B.C.Z., Y.H.Z), Chinese Academy of Sciences, Beijing 100101, China;Department of Plant and Microbial Biology University of California, Berkeley, California, 94720 (N.M.);Department of Plant Physiology and Nutrition, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China (Y.Z.S.).
authors.forename: Nasim
authors.initials: N
authors.lastname: Mansoori
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences (X.F.Z., Y.S, J.X.W., Y.L., Z.W.W., S.J.Z.), and State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (B.C.Z., Y.H.Z), Chinese Academy of Sciences, Beijing 100101, China;Department of Plant and Microbial Biology University of California, Berkeley, California, 94720 (N.M.);Department of Plant Physiology and Nutrition, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China (Y.Z.S.).
authors.forename: Jiang Xue
authors.initials: JX
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences (X.F.Z., Y.S, J.X.W., Y.L., Z.W.W., S.J.Z.), and State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (B.C.Z., Y.H.Z), Chinese Academy of Sciences, Beijing 100101, China;Department of Plant and Microbial Biology University of California, Berkeley, California, 94720 (N.M.);Department of Plant Physiology and Nutrition, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China (Y.Z.S.).
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences (X.F.Z., Y.S, J.X.W., Y.L., Z.W.W., S.J.Z.), and State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (B.C.Z., Y.H.Z), Chinese Academy of Sciences, Beijing 100101, China;Department of Plant and Microbial Biology University of California, Berkeley, California, 94720 (N.M.);Department of Plant Physiology and Nutrition, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China (Y.Z.S.).
authors.forename: Zhi Wei
authors.initials: ZW
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences (X.F.Z., Y.S, J.X.W., Y.L., Z.W.W., S.J.Z.), and State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (B.C.Z., Y.H.Z), Chinese Academy of Sciences, Beijing 100101, China;Department of Plant and Microbial Biology University of California, Berkeley, California, 94720 (N.M.);Department of Plant Physiology and Nutrition, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China (Y.Z.S.).
authors.forename: Yuan Zhi
authors.initials: YZ
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences (X.F.Z., Y.S, J.X.W., Y.L., Z.W.W., S.J.Z.), and State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (B.C.Z., Y.H.Z), Chinese Academy of Sciences, Beijing 100101, China;Department of Plant and Microbial Biology University of California, Berkeley, California, 94720 (N.M.);Department of Plant Physiology and Nutrition, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China (Y.Z.S.).
authors.forename: Yi Hua
authors.initials: YH
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences (X.F.Z., Y.S, J.X.W., Y.L., Z.W.W., S.J.Z.), and State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (B.C.Z., Y.H.Z), Chinese Academy of Sciences, Beijing 100101, China;Department of Plant and Microbial Biology University of California, Berkeley, California, 94720 (N.M.);Department of Plant Physiology and Nutrition, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China (Y.Z.S.) sjzheng@zju.edu.cn.
authors.forename: Shao Jian
authors.initials: SJ
authors.lastname: Zheng
mesh_terms: D000107:Acetylation; D000535:Aluminum / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D005936:Glucans / Q000378:metabolism*; D045565:Holliday Junction Resolvases / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; C007916:hemicellulose; D000535:Aluminum; D045565:Holliday Junction Resolvases; C000602688:TRICHOME BIREFRINGENCE-LIKE27 protein, Arabidopsis