title: Phasic study of intestinal homeostasis disruption in experimental intestinal obstruction.
abstract: OBJECTIVE
  To investigate the phasic alteration of intestinal homeostasis in an experimental model of intestinal obstruction.
  
  
  METHODS
  A rabbit model of intestinal obstruction was established by transforming parts of an infusion set into an in vivo pulled-type locking clamp and creating a uniform controllable loop obstruction in the mesenteric non-avascular zone 8 cm from the distal end of the ileum. The phasic alteration of intestinal homeostasis was studied after intestinal obstruction. The changes in goblet cells, intraepithelial lymphocytes, lamina propria lymphocytes, and intestinal epithelium were quantified from periodic acid-Schiff-stained sections. Ornithine decarboxylase (ODC) activity and serum citrulline levels were measured by high-performance liquid chromatography. Claudin 1 mRNA expression was examined by real-time polymerase chain reaction analysis. Intestinal microorganisms, wet/dry weight ratios, pH values, and endotoxin levels were determined at multiple points after intestinal obstruction. Furthermore, the number and ratio of CD3(+), CD4(+) and CD8(+) T cells were determined by flow cytometry, and secretory IgA levels were measured with an enzyme-linked immunosorbent assay.
  
  
  RESULTS
  A suitable controllable rabbit model of intestinal obstruction was established. Intestinal obstruction induced goblet cell damage and reduced cell number. Further indicators of epithelial cell damage were observed as reduced serum citrulline levels and claudin 1 gene expression, and a transient increase in ODC activity. In addition, the wet/dry weight ratio and pH of the intestinal lumen were also dramatically altered. The ratio of Bacillus bifidus and enterobacteria was reversed following intestinal obstruction. The number and area of Peyer's patches first increased then sharply decreased after the intestinal obstruction, along with an alteration in the ratio of CD4/CD8(+) T cells, driven by an increase in CD3(+) and CD8(+) T cells and a decrease in CD4(+) T cells. The number of lamina propria lymphocytes also gradually decreased with prolonged obstruction.
  
  
  CONCLUSIONS
  Intestinal obstruction can induce disruption of intestinal homeostasis.
authors.affiliation: Xiang-Yang Yu, Zhen-Li Zhou, Tao Shan, Department of Gastrointestinal Surgery, Nankai Hospital, Tianjin 300100, China.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Yu
authors.affiliation: Xiang-Yang Yu, Zhen-Li Zhou, Tao Shan, Department of Gastrointestinal Surgery, Nankai Hospital, Tianjin 300100, China.
authors.forename: Chang-Lin
authors.initials: CL
authors.lastname: Zou
authors.affiliation: Xiang-Yang Yu, Zhen-Li Zhou, Tao Shan, Department of Gastrointestinal Surgery, Nankai Hospital, Tianjin 300100, China.
authors.forename: Zhen-Li
authors.initials: ZL
authors.lastname: Zhou
authors.affiliation: Xiang-Yang Yu, Zhen-Li Zhou, Tao Shan, Department of Gastrointestinal Surgery, Nankai Hospital, Tianjin 300100, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Shan
authors.affiliation: Xiang-Yang Yu, Zhen-Li Zhou, Tao Shan, Department of Gastrointestinal Surgery, Nankai Hospital, Tianjin 300100, China.
authors.forename: Dong-Hua
authors.initials: DH
authors.lastname: Li
authors.affiliation: Xiang-Yang Yu, Zhen-Li Zhou, Tao Shan, Department of Gastrointestinal Surgery, Nankai Hospital, Tianjin 300100, China.
authors.forename: Nai-Qiang
authors.initials: NQ
authors.lastname: Cui
mesh_terms: D000818:Animals; D001407:Bacillus / Q000254:growth & development; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D004755:Enterobacteriaceae / Q000254:growth & development; D006706:Homeostasis; D007077:Ileal Diseases / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D007082:Ileum / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D007415:Intestinal Obstruction / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D011817:Rabbits; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013997:Time Factors
chemical_list: D015415:Biomarkers
keywords: Homeostasis disruption; Intestinal epithelial cells; Intestinal immune system; Intestinal microorganisms; Intestinal obstruction; Rabbit model