title: Cryopreservation of quince (Cydonia oblonga Mill.).
abstract: BACKGROUND
  Quince (Cydonia oblonga Mill.) has great potential for utilisation in pharmaceutical and food industries.
  
  
  OBJECTIVE
  The study was to develop an efficient cryopreservation approach for quince.
  
  
  METHODS
  Factors on the survival and regrowth such as cold acclimation, explant type and recovery media composition were assessed. The effectiveness of the resultant protocols for a number of quince cultivars was determined.
  
  
  CONCLUSIONS
  Quince shoot tips and nodal sections are successfully cryopreserved. Sustained regrowth of quince Angers A was observed after encapsulation-osmoprotection/dehydration, encapsulation-dehydration and PVS2 vitrification. The highest regrowth rate (80%) was obtained from explants excised from cold hardened shoots and cryopreserved using encapsulation-osmoprotection/dehydration and vitrification protocols. The optimised vitrification protocol in combination with shoot cold hardening and a MS recovery medium without activated charcoal and auxin resulted in satisfactory regrowth of shoots from six quince cultivars. The morphology of acclimatised plants derived from cryopreserved shoots was comparable with non-cryopreserved plants.
authors.affiliation: Biological Sciences Research Group, University of Derby, Derby, UK. P.T.Lynch@derby.ac.uk.
authors.forename: P T
authors.initials: PT
authors.lastname: Lynch
authors.affiliation: Biological Sciences Research Group, University of Derby, Derby, U.
authors.forename: A
authors.initials: A
authors.lastname: Siddika
authors.affiliation: Biological Sciences Research Group, University of Derby, Derby, U.
authors.forename: A
authors.initials: A
authors.lastname: Mehra
authors.affiliation: Istituto per la Valorizzazione del Legno e delle Specie Arboree, (IVALSA), National Research Council, (Firenze) Italy.
authors.forename: C
authors.initials: C
authors.lastname: Benelli
authors.affiliation: Istituto per la Valorizzazione del Legno e delle Specie Arboree, (IVALSA), National Research Council, (Firenze) Italy.
authors.forename: M
authors.initials: M
authors.lastname: Lambardi
mesh_terms: D000064:Acclimatization; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000737:chemistry; D005838:Genotype; D064587:Osmoregulation; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D027824:Rosaceae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents