title: RhoE deficiency alters postnatal subventricular zone development and the number of calbindin-expressing neurons in the olfactory bulb of mouse.
abstract: The subventricular zone represents an important reservoir of progenitor cells in the adult brain. Cells from the subventricular zone migrate along the rostral migratory stream and reach the olfactory bulb, where they originate different types of interneurons. In this work, we have analyzed the role of the small GTPase RhoE/Rnd3 in subventricular zone cell development using mice-lacking RhoE expression. Our results show that RhoE null mice display a remarkable postnatal broadening of the subventricular zone and caudal rostral migratory stream. This broadening was caused by an increase in progenitor proliferation, observed in the second postnatal week but not before, and by an altered migration of the cells, which appeared in disorganized cell arrangements that impaired the appropriate contact between cells in the rostral migratory stream. In addition, the thickness of the granule cell layer in the olfactory bulb was reduced, although the density of granule cells did not differ between wild-type and RhoE null mice. Finally, the lack of RhoE expression affected the olfactory glomeruli inducing a severe reduction of calbindin-expressing interneurons in the periglomerular layer. This was already evident in the newborns and even more pronounced 15 days later when RhoE null mice displayed 89% less cells than control mice. Our results indicate that RhoE has pleiotropic functions on subventricular cells because of its role in proliferation and tangential migration, affecting mainly the development of calbindin-expressing cells in the olfactory bulb.
authors.affiliation: Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain.|Instituto de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Ballester-Lurbe
authors.affiliation: Laboratorio de Neurobiologia Comparada, Instituto Cavanilles de Biodiversidad y Biologia Evolutiva, Universidad de Valencia and CIBER de Enfermedades Neurodegenerativas (CIBERNED), Valencia, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: González-Granero
authors.affiliation: Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain.|Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, NY, 10461, USA.
authors.forename: Enric
authors.initials: E
authors.lastname: Mocholí
authors.affiliation: Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain.|Instituto de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain.
authors.forename: Enric
authors.initials: E
authors.lastname: Poch
authors.affiliation: Instituto de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain.|Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad CEU Cardenal Herrera, Avda Seminario s.n., 46113, Moncada, Valencia, Spain.
authors.forename: María
authors.initials: M
authors.lastname: García-Manzanares
authors.affiliation: Systems Biology Program, Center for Genomic Regulation (CRG), Barcelona, Spain.|University Pompeu Fabra, Barcelona, Spain.|CIBER de Enfermedades Raras (CIBERER), Barcelona, Spain.
authors.forename: Mara
authors.initials: M
authors.lastname: Dierssen
authors.affiliation: Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain.|Instituto de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Pérez-Roger
authors.affiliation: Laboratorio de Neurobiologia Comparada, Instituto Cavanilles de Biodiversidad y Biologia Evolutiva, Universidad de Valencia and CIBER de Enfermedades Neurodegenerativas (CIBERNED), Valencia, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: García-Verdugo
authors.affiliation: Laboratorio de Señalización de Proteínas Rho en Neuropatologías, Centro de Investigación Príncipe Felipe, Valencia, Spain.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Guasch
authors.affiliation: Instituto de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain. jterrado@uch.ceu.es.|Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad CEU Cardenal Herrera, Avda Seminario s.n., 46113, Moncada, Valencia, Spain. jterrado@uch.ceu.es.
authors.forename: José
authors.initials: J
authors.lastname: Terrado
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D064026:Calbindins / Q000096:biosynthesis* / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D020547:Lateral Ventricles / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism*; D065820:Zona Incerta / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000172:deficiency* / Q000378:metabolism*
chemical_list: D064026:Calbindins; C513341:Rnd3 protein, mouse; D020741:rho GTP-Binding Proteins
keywords: Calbindin; Proliferation; RhoE; Rnd proteins; Rostral migratory stream; Subventricular zone