title: Dual role of MsRbpA: transcription activation and rescue of transcription from the inhibitory effect of rifampicin.
abstract: MsRbpA is an RNA polymerase (RNAP) binding protein from Mycobacterium smegmatis. According to previous studies, MsRbpA rescues rifampicin-induced transcription inhibition upon binding to the RNAP. Others have shown that RbpA from Mycobacterium tuberculosis (MtbRbpA) is a transcription activator. In this study, we report that both MsRbpA and MtbRbpA activate transcription as well as rescue rifampicin-induced transcription inhibition. Transcription activation is achieved through the increased formation of closed RNAP-promoter complex as well as enhanced rate of conversion of this complex to a stable transcriptionally competent RNAP-promoter complex. When a 16 aa peptide fragment (Asp 58 to Lys 73) was deleted from MsRbpA, the resulting protein showed 1000-fold reduced binding with core RNAP. The deletion results in abolition of transcription activation and rescue of transcription from the inhibitory effect of rifampicin. Through alanine scanning of this essential region of MsRbpA, Gly 67, Val 69, Pro 70 and Pro 72 residues are identified to be important for MsRbpA function. Furthermore, we report here that the protein is indispensable for M. smegmatis, and it appears to help the organism grow in the presence of the antibiotic rifampicin.
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, Karnataka-560012, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Verma
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, Karnataka-560012, India.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Chatterji
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D004252:DNA Mutational Analysis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000201:enzymology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D011485:Protein Binding; D012293:Rifampin / Q000494:pharmacology*; D017384:Sequence Deletion; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D000995:Antitubercular Agents; D050505:Mutant Proteins; D015534:Trans-Activators; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin