title: Aqueous and lipid nuclear magnetic resonance metabolomic profiles of the earthworm Aporrectodea caliginosa show potential as an indicator species for environmental metabolomics.
abstract: The common pasture earthworm Aporrectodea caliginosa has often been neglected in environmental metabolomics in favor of species easily bred in the laboratory. The present study assigns aqueous metabolites in A. caliginosa using high-resolution 1- and 2-dimensional nuclear magnetic resonance (NMR) spectroscopy. In total, 51 aqueous metabolites were identified, including typical amino acids (alanine, leucine, asparagine, phenylalanine), sugars (maltose, glucose), the dominant earthworm-specific 2-hexyl-5-ethyl-furansulfonate, and several previously unreported metabolites (oxoglutarate, putrescine). Examining the lesser-known earthworm lipid metabolome showed various lipid fatty acyl chains, cholesterol, and phosphatidylcholine. To briefly test if the NMR metabolomic techniques could differentiate A. caliginosa from different sites, earthworms were collected from 2 adjacent farms. Orthogonal partial least squares discriminant analysis detected metabolomic differences, suggesting the worms from the 2 sites differed in their energy metabolism, as indicated by altered levels of alanine, glutamine, glutamate, malate, fumarate, and lipids. Evidence of greater utilization of lipid energy reserves and onset of protein catabolism was also present. While the precise cause of the metabolomic differences could not be determined, the results show the potential of this species for further environmental metabolomic studies.
authors.affiliation: Institute for Neurosciences and Physiology, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Brown
authors.forename: Linda
authors.initials: L
authors.lastname: Samuelsson
authors.forename: Giuliana
authors.initials: G
authors.lastname: Bernardi
authors.forename: Ravi
authors.initials: R
authors.lastname: Gooneratne
authors.forename: D G Joakim
authors.initials: DG
authors.lastname: Larsson
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D004734:Energy Metabolism; D004777:Environment; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D009835:Oligochaeta / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D008055:Lipids
keywords: Amino acid; Aporrectodea caliginosa; Earthworm; Lipid; Nuclear magnetic resonance metabolomics