title: Tissue oxygen saturation mapping with magnetic resonance imaging.
abstract: A quantitative estimate of cerebral blood oxygen saturation is of critical importance in the investigation of cerebrovascular disease. While positron emission tomography can map in vivo the oxygen level in blood, it has limited availability and requires ionizing radiation. Magnetic resonance imaging (MRI) offers an alternative through the blood oxygen level-dependent contrast. Here, we describe an in vivo and non-invasive approach to map brain tissue oxygen saturation (StO2) with high spatial resolution. StO2 obtained with MRI correlated well with results from blood gas analyses for various oxygen and hematocrit challenges. In a stroke model, the hypoxic areas delineated in vivo by MRI spatially matched those observed ex vivo by pimonidazole staining. In a model of diffuse traumatic brain injury, MRI was able to detect even a reduction in StO2 that was too small to be detected by histology. In a F98 glioma model, MRI was able to map oxygenation heterogeneity. Thus, the MRI technique may improve our understanding of the pathophysiology of several brain diseases involving impaired oxygenation.
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Christen
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France [3] CHU Grenoble, Pôle d'Anesthésie-Réanimation, Grenoble, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Bouzat
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pannetier
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Coquery
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.
authors.forename: Anaïck
authors.initials: A
authors.lastname: Moisan
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Lemasson
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France [3] CHU Grenoble, Pôle d'Anesthésie-Réanimation, Grenoble, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Thomas
authors.affiliation: 1] Inserm, US 017, Grenoble, France [2] Université Grenoble Alpes, IRMaGe, Grenoble, France [3] CHU Grenoble, IRMaGe, Grenoble, France [4] CNRS, UMS 3552, Grenoble, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Grillon
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France [3] CHU Grenoble, Service de Neurologie, Grenoble, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Detante
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Rémy
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France [3] CHU Grenoble, Pôle d'Anesthésie-Réanimation, Grenoble, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Payen
authors.affiliation: 1] Inserm, U836, Grenoble, France [2] Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.
authors.forename: Emmanuel Luc
authors.initials: EL
authors.lastname: Barbier
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis / Q000379:methods; D001930:Brain Injuries / Q000000981:diagnostic imaging* / Q000378:metabolism*; D004195:Disease Models, Animal; D008279:Magnetic Resonance Imaging; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D011859:Radiography; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar; D020521:Stroke / Q000000981:diagnostic imaging* / Q000378:metabolism*
chemical_list: D010100:Oxygen