title: Olive, grass or both? Molecular diagnosis for the allergen immunotherapy selection in polysensitized pollinic patients.
abstract: BACKGROUND
  Grass and olive are the most frequently pollens that induce seasonal allergic rhinitis in Spain. Cross-reactivity due to panallergens shared by them and overlapping pollination complicates the recognition of allergy-causing agents, making it difficult to identify the most appropriate allergen immunotherapy (AIT) to use. The aim of this study was to determine the sensitization pattern to major grass and olive pollen allergens using component-resolved diagnostics in patients with seasonal allergic rhinitis (SAR) and positive skin prick test to grass and olive pollens and evaluate how knowledge of the sensitization patterns might influence AIT prescription.
  
  
  METHODS
  After informed written consent, a total of 1263 patients were recruited. A serum determination of specific IgE levels to Ole e 1 and Phl p 1 + 5 was performed to all patients. A comparison was made before and after obtaining the specific IgE results, and differences in diagnosis were stated.
  
  
  RESULTS
  At the 0.35 kU/l cut-off point, 71.2% of patients were positive to Ole e 1 and Phl p 1 + 5, 14% were positive only to Phl p 1 + 5 and 12% were positive only to Ole e 1. Based on available clinical data and skin prick test results, 922 (73%) patients would have been indicated for a mixture of grass and olive pollens for AIT. In 56.8% of patients, there was non-coincidence in the composition of AIT that would be selected before and after investigators received the in vitro data.
  
  
  CONCLUSIONS
  The diagnostic accuracy of the recombinant allergen-specific IgE test could help to improve the selection of specific-allergen immunotherapy in polysensitized patients.
authors.affiliation: Hospital Universitario Reina Sofía, Cordoba, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Moreno
authors.forename: J L
authors.initials: JL
authors.lastname: Justicia
authors.forename: J
authors.initials: J
authors.lastname: Quiralte
authors.forename: A
authors.initials: A
authors.lastname: Moreno-Ancillo
authors.forename: A
authors.initials: A
authors.lastname: Iglesias-Cadarso
authors.forename: M
authors.initials: M
authors.lastname: Torrecillas
authors.forename: N
authors.initials: N
authors.lastname: Labarta
authors.forename: M A
authors.initials: MA
authors.lastname: García
authors.forename: I
authors.initials: I
authors.lastname: Dávila
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D003888:Desensitization, Immunologic / Q000379:methods*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008875:Middle Aged; D031658:Olea; D010940:Plant Proteins / Q000276:immunology*; D006109:Poaceae; D011446:Prospective Studies; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology* / Q000517:prevention & control; D012882:Skin Tests; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C081946:Ole e I protein, Olea europaea; D010940:Plant Proteins; C090396:PHLPI protein, Phleum pratense; D007073:Immunoglobulin E
keywords: allergens and epitopes; clinical immunology; immunologic tests; immunotherapy and tolerance induction