title: Cohnella nanjingensis sp. nov., an extracellular polysaccharide-producing bacterium isolated from soil.
abstract: A Gram-stain-positive, rod-shaped, endospore-forming bacterium, strain D45(T), was isolated from soil in Nanjing, China. The organism grew optimally at 30 °C, pH 7.0 and with 0 % NaCl (w/v). The 16S rRNA gene sequence of the isolate showed similarities lower than 97 % with respect to species of the genus Cohnella. The predominant respiratory quinone was MK-7, with MK-6 present as a minor component; anteiso-C15 : 0 and iso-C16 : 0 were the major fatty acids. The polar lipids of strain D45(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, four phospholipids, two glycolipids, one aminolipid and two lipids. The DNA G+C content was 59.5 mol%. DNA-DNA hybridization of the isolate with two reference strains showed relatedness values of 33.4 % with Cohnella ginsengisoli DSM 18997(T) and 25.8 % with Cohnella thermotolerans DSM 17683(T). The phylogenetic, chemotaxonomic and phenotypic data supported the classification of strain D45(T) as a representative of a novel species of the genus Cohnella, for which the name Cohnella nanjingensis sp. nov. is proposed. The type strain is D45(T) ( = CCTCC AB 2014067(T) = DSM 28246(T)).
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Yu
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Bao
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xia
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xia-Fang
authors.initials: XF
authors.lastname: Sheng
authors.affiliation: Key Laboratory of Microbiology of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Lin-Yan
authors.initials: LY
authors.lastname: He
mesh_terms: D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6; C062629:menaquinone 7