title: Transcriptomic, biochemical and individual markers in transplanted Daphnia magna to characterize impacts in the field.
abstract: Daphnia magna individuals were transplanted across 12 sites from three Spanish river basins (Llobregat, Ebro, Jucar) showing different sources of pollution. Gene transcription, feeding and biochemical responses in the field were assessed and compared with those obtained in re-constituted water treatments spiked with organic eluates obtained from water samples collected at the same locations and sampling periods. Up to 166 trace contaminants were detected in water and classified by their mode of action into 45 groups that included metals, pharmaceuticals, pesticides, illicit drugs, and other industrial compounds. Physicochemical water parameters differentiated the three river basins with Llobregat having the highest levels of conductivity, metals and pharmaceuticals, followed by Ebro, whereas the Jucar river had the greatest levels of illicit drugs. D. magna grazing rates and cholinesterase activity responded similarly than the diversity of riparian benthic communities. Transcription patterns of 13 different genes encoding for general stress, metabolism and energy processes, molting and xenobiotic transporters corroborate phenotypic responses differentiated sites within and across river basins. Principal Component Analysis and Partial Least Square Projections to Latent Structures regression analyses indicated that measured in situ responses of most genes and biomarkers and that of benthic macroinvertebrate diversity indexes were affected by distinct environmental factors. Conductivity, suspended solids and fungicides were negatively related with the diversity of macroinvertebrates cholinesterase, and feeding responses. Gene transcripts of heat shock protein and metallothionein were positively related with 11 classes of organic contaminants and 6 metals. Gene transcripts related with signaling paths of molting and reproduction, sugar, protein and xenobiotic metabolism responded similarly in field and lab exposures and were related with high residue concentrations of analgesics, diuretics, psychiatric drugs, β blockers, illicit drugs, trizoles, bisphenol A, caffeine and pesticides. These results indicate that application of omic technologies in the field is a promising subject in water management.
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Claudia
authors.initials: C
authors.lastname: Rivetti
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Bruno
authors.initials: B
authors.lastname: Campos
authors.affiliation: CESAM, Departamento de Biologia, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Melissa
authors.initials: M
authors.lastname: Faria
authors.affiliation: Department of Ecology, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: De Castro Català
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Amrita
authors.initials: A
authors.lastname: Malik
authors.affiliation: Department of Ecology, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Muñoz
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Romà
authors.initials: R
authors.lastname: Tauler
authors.affiliation: CESAM, Departamento de Biologia, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Amadeu M V M
authors.initials: AM
authors.lastname: Soares
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: Osorio
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Pérez
authors.affiliation: Catalan Institute of Water Research, ICRA, C/Emili Grahit, 101, Edifici H2O, Parc Científic i Tecnològic de la Universitat de Girona, E-17003 Girona, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Gorga
authors.affiliation: Catalan Institute of Water Research, ICRA, C/Emili Grahit, 101, Edifici H2O, Parc Científic i Tecnològic de la Universitat de Girona, E-17003 Girona, Spain; ICREA, Catalan Institution for Research and Advanced Studies, Barcelona, Spain.
authors.forename: Mira
authors.initials: M
authors.lastname: Petrovic
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Nicola
authors.initials: N
authors.lastname: Mastroianni
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Miren López
authors.initials: ML
authors.lastname: de Alda
authors.affiliation: Food and Environmental Safety Research Group (SAMA-UV), Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n., 46100 Burjassot, València, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Masiá
authors.affiliation: Food and Environmental Safety Research Group (SAMA-UV), Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n., 46100 Burjassot, València, Spain.
authors.forename: Julian
authors.initials: J
authors.lastname: Campo
authors.affiliation: Food and Environmental Safety Research Group (SAMA-UV), Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n., 46100 Burjassot, València, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Picó
authors.affiliation: Food and Environmental Safety Research Group (SAMA-UV), Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n., 46100 Burjassot, València, Spain; University of Girona, Campus Montilivi, 17071 Girona, Spain.
authors.forename: Helena
authors.initials: H
authors.lastname: Guasc
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
authors.forename: Damià
authors.initials: D
authors.lastname: Barceló
authors.affiliation: Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain. Electronic address: cbmqam@cid.csic.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: Barata
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003621:Daphnia; D004784:Environmental Monitoring / Q000379:methods*; D010575:Pesticides / Q000633:toxicity; D013030:Spain; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D015415:Biomarkers; D010575:Pesticides; D014874:Water Pollutants, Chemical
keywords: Biomarker; Daphnia; Feeding; Gene; Transcriptomics; Water quality