title: MicroRNA-27b suppresses growth and invasion of NSCLC cells by targeting Sp1.
abstract: Non-small cell lung cancer (NSCLC) is the major cause of cancer death worldwide. Increasing evidence shows that microRNAs (miRNAs), evolutionally conserved non-coding RNAs, are widely involved in the development and progression of NSCLC. Aberrant alteration of miRNAs expression has been implicated in NSCLC initiation and progression. Herein, we studied the role of miR-27b in NSCLC cells. We found that miR-27b was significantly decreased in several NSCLC cell lines. Forced overexpression of miR-27 inhibited both the growth and invasion of NSCLC cells. Furthermore, we identified Sp1 transcription factor (Sp1) as a target of miR-27b in NSCLC cells. Moreover, we found that miR-27 suppressed growth and invasion of NSCLC cells partially by targeting Sp1. Our data indicate that miR-27b may play a critical role in the development of NSCLC.
authors.affiliation: Department of Oncology, The Central Hospital of Wuhan, 430014, Wuhan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Lv
authors.forename: Liang
authors.initials: L
authors.lastname: Fan
authors.forename: Guodong
authors.initials: G
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhan
authors.forename: Mengyi
authors.initials: M
authors.lastname: Wang
authors.forename: Hongda
authors.initials: H
authors.lastname: Lu
mesh_terms: D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016329:Sp1 Transcription Factor / Q000378:metabolism*
chemical_list: C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D016329:Sp1 Transcription Factor; C108209:SP1 protein, human