title: TGF-β detection and measurement in murine B cells: pros and cons of the different techniques.
abstract: Recent studies have demonstrated the importance of regulatory B cells in autoimmune, allergic, and inflammatory diseases. These B cells have an ability to suppress excessive immune reactions by multiple mechanisms. Most studies have focused on IL-10-producing B cells, but we have previously reported that transforming growth factor (TGF)-β1 secretion by B cells also plays an important role in intestinal homeostasis and mucosal inflammation. B cell-secreted TGF-β may be involved in the regulation of T cell immunity (differentiation, proliferation, and apoptosis) that is relevant to the pathogenesis of autoimmune or inflammatory disease. Here, we provide detailed instruction for detecting and measuring TGF-β produced by B cells.
authors.affiliation: Faculty of Medicine, Department of Internal Medicine II, Shimane University, 89-1 Enya-cho, Izumo-shi, Shimane, 693-8501, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Mishima
authors.forename: Shunji
authors.initials: S
authors.lastname: Ishihara
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Hansen
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kinoshita
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000235:genetics / Q000276:immunology
chemical_list: D012333:RNA, Messenger; D016212:Transforming Growth Factor beta