title: Multivalent capture and detection of cancer cells with DNA nanostructured biosensors and multibranched hybridization chain reaction amplification.
abstract: Sensitive detection of cancer cells plays a critically important role in the early detection of cancer and cancer metastasis. However, because circulating tumor cells are extremely rare in peripheral blood, the detection of cancer cells with high analytical sensitivity and specificity remains challenging. Here, we have demonstrated a simple, sensitive and specific detection of cancer cells with the detection sensitivity of four cancer cells, which is lower than the cutoff value with respect to correlation with survival outcomes as well as predictive of metastatic disease in clinical diagnostics. We re-engineered the hybridization chain reaction (HCR) to multibranched HCR (mHCR) that can produce long products with multiple biotins for signal amplification and multiple branched arms for multivalent binding. The capturing gold surface is modified with DNA tetrahedral probes, which provide superior hybridization conditions for the multivalent binding. The synergetic effect of mHCR amplification and multivalent binding lead to the high sensitivity of our detection platform.
authors.affiliation: Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai, China 201800.
authors.forename: Guobao
authors.initials: G
authors.lastname: Zhou
authors.forename: Meihua
authors.initials: M
authors.lastname: Lin
authors.forename: Ping
authors.initials: P
authors.lastname: Song
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Chao
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000473:pathology*; D017931:DNA Primers; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D049329:Nanostructures; D009693:Nucleic Acid Hybridization
chemical_list: D017931:DNA Primers