title: Spatial and temporal changes of decorin, type I collagen and fibronectin expression in normal and clone bovine placenta.
abstract: BACKGROUND
  Alteration of expression of various genes including extracellular matrix components, have been suggested to play major role in the placental pathologies after somatic cloning in mammals. The objectives of the present study were to analyze pattern of expression (mRNA and protein) of the small leucine-rich proteoglycan, Decorin in association with Type I Collagen and Fibronectin in bovine placental tissues from normal and clone pregnancies.
  
  
  METHODS
  Genotyping and allelic expression of Decorin were determined by Sanger sequencing. The expression patterns of Decorin, Type I collagen and Fibronectin 1 were analyzed by quantitative RT-qPCR and combined in situ hybydization (ISH) and immunohistochemistry (IHC) in endometrial and placental tissues from D18 to term from artificially inseminated and somatic cloning pregnancies.
  
  
  RESULTS
  The expression levels of DCN increased in the AI endometrial stroma and chorionic mesenchyme during implantation and declined during placentome growth until term. Combined ISH and IHC revealed an unexpected discrepancy mRNA and protein tissue distribution. Moreover, Decorin was maintained in the placentome tissues from SCNT pregnancies while both mRNA and protein were absent in AI derived placenta.
  
  
  CONCLUSIONS
  In bovine, the pattern of expression of Decorin exhibits significant changes during placental formation. Downregulation of Decorin is associated with proliferation, remodeling and vascularization of placental tissues. These observations reinforces the putative role of Decorin in these processes.
  
  
  CONCLUSIONS
  These observations suggest that Decorin is involved in placental growth and that dysregulation of its expression is associated with placental abnormalities in SCNT derived pregnancy.
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France. Electronic address: michel.guillomot@jouy.inra.fr.
authors.forename: M
authors.initials: M
authors.lastname: Guillomot
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: E
authors.initials: E
authors.lastname: Campion
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: A
authors.initials: A
authors.lastname: Prézelin
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: O
authors.initials: O
authors.lastname: Sandra
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: I
authors.initials: I
authors.lastname: Hue
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: D
authors.initials: D
authors.lastname: Le Bourhis
authors.affiliation: INRA, UE1298 Unité Commune d'Expérimentation Animale, F-91630 Leudeville, France.
authors.forename: C
authors.initials: C
authors.lastname: Richard
authors.affiliation: Institute for Genomic Biology, Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61820, USA.
authors.forename: F H
authors.initials: FH
authors.lastname: Biase
authors.affiliation: Institute for Genomic Biology, Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61820, USA.
authors.forename: C
authors.initials: C
authors.lastname: Rabel
authors.affiliation: College of Veterinary Medicine, University of Illinois at Urbana-Champaign, USA.
authors.forename: R
authors.initials: R
authors.lastname: Wallace
authors.affiliation: Institute for Genomic Biology, Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61820, USA; Department of Evolution and Ecology and The Genome Center, University of California, Davis, CA 95616, USA.
authors.forename: H
authors.initials: H
authors.lastname: Lewin
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: J-P
authors.initials: JP
authors.lastname: Renard
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: H
authors.initials: H
authors.lastname: Jammes
mesh_terms: D000818:Animals; D002417:Cattle; D019976:Cloning, Organism; D024042:Collagen Type I / Q000378:metabolism*; D058575:Decorin / Q000378:metabolism*; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D018392:Genomic Imprinting; D007315:Insemination, Artificial; D053652:Nuclear Transfer Techniques; D010920:Placenta / Q000378:metabolism*; D010929:Placentation; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D058575:Decorin; D005353:Fibronectins; D012333:RNA, Messenger
keywords: Cloning; Extracellular matrix; Imprinting; Placenta; Ruminants