title: Inhibition of FoxO1 acetylation by INHAT subunit SET/TAF-Iβ induces p21 transcription.
abstract: Post-translational modification of forkhead family transcription factor, FoxO1, is an important regulatory mode for its diverse activities. FoxO1 is acetylated by HAT coactivators and its transcriptional activity is decreased via reduced DNA binding affinity. Here, we report that SET/TAF-Iβ inhibited p300-mediated FoxO1 acetylation in an INHAT domain-dependent manner. SET/TAF-Iβ interacted with FoxO1 and activated transcription of FoxO1 target gene, p21. Moreover, SET/TAF-Iβ inhibited acetylation of FoxO1 and increased p21 transcription induced by oxidative stress. Our results suggest that SET/TAF-Iβ inhibits FoxO1 acetylation and activates its transcriptional activity toward p21.
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Yun-Cheol
authors.initials: YC
authors.lastname: Chae
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Kee-Beom
authors.initials: KB
authors.lastname: Kim
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Kang
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Se-Ryeon
authors.initials: SR
authors.lastname: Kim
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Hyeon-Soo
authors.initials: HS
authors.lastname: Jung
authors.affiliation: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea. Electronic address: sangbs@cau.ac.kr.
authors.forename: Sang-Beom
authors.initials: SB
authors.lastname: Seo
mesh_terms: D000107:Acetylation; D017209:Apoptosis; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D004268:DNA-Binding Proteins; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D005786:Gene Expression Regulation; D045325:HCT116 Cells; D057809:HEK293 Cells; D056488:Histone Chaperones / Q000378:metabolism*; D006801:Humans; D018384:Oxidative Stress; D021122:Protein Subunits / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D004268:DNA-Binding Proteins; C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D056488:Histone Chaperones; D021122:Protein Subunits; C513473:SET protein, human; D014157:Transcription Factors
keywords: Acetylation; FoxO1; SET/TAF-Iβ; Transcription; p21