title: CCL20/CCR6 promotes the invasion and migration of thyroid cancer cells via NF-kappa B signaling-induced MMP-3 production.
abstract: CCL20, an important member of the CC-chemokine family, is the only ligand that activates CCR6. The levels of CCL20 and CCR6 are elevated in many human cancers, and CCL20/CCR6 interaction participates in the development and progression of cancer. In this present study, we found that CCR6 was overexpressed in thyroid cancer cells. Activation of CCR6 by CCL20 promoted the invasion and migration of human thyroid cancer SW1736 cells, while knockdown of CCR6 repressed the effect of CCL20. Furthermore, CCL20/CCR6 interaction induced the activation of NF-κB, and stimulated the expression and secretion of MMP-3. In addition, BAY117082, a special inhibitor of NF-κB, suppressed the expression and secretion of MMP-3 stimulated by CCL20/CCR6. Together, these results suggest that CCL20/CCR6 enhances thyroid cancer cell invasion and migration. The possible molecular mechanisms involved NF-κB activation and NF-κB-dependent MMP-3 upregulation. Thus, molecular therapies that aim at CCL20 and CCR6 may offer promising intervention strategies for thyroid cancer.
authors.affiliation: Department of Otolaryngology, First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Department of Otolaryngology, First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chang
authors.affiliation: Department of Otolaryngology, First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Min
authors.initials: M
authors.lastname: Ma
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, China. Electronic address: lywyan78@163.com.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D054418:Chemokine CCL20 / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D054399:Receptors, CCR6 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D015854:Up-Regulation
chemical_list: C108355:CCL20 protein, human; C106311:CCR6 protein, human; D054418:Chemokine CCL20; D016328:NF-kappa B; D054399:Receptors, CCR6; D019278:Matrix Metalloproteinase 3
keywords: CCL20; CCR6; Invasion; NF-κB; Thyroid cancer