title: Production of goat milk protein hydrolysate enriched in ACE-inhibitory peptides by ultrafiltration.
abstract: A global process for the production of goat milk hydrolysates enriched in angiotensin converting enzyme (ACE) inhibitory peptides was proposed. Firstly, the protein fractions (caseins and whey proteins) were separated by ultrafiltration through a 0·14 μm ceramic membrane. The casein fraction obtained in the retentate stream of the above filtration step was subsequently hydrolysed with a combination of subtilisin and trypsin. After 3 h of reaction, the hydrolysate produced presented an IC50 of 218·50 μg/ml, which represent a relatively high ACE inhibitory activity. Finally, this hydrolysate was filtered through a 50 kDa ceramic membrane until reaching a volume reduction factor of 3. The permeate produced presented an improvement of more than 30% in the ACE inhibitory activity. In contrast, the retentate was concentrated in larger and inactive peptides which led to a decrease of more than 80% in its inhibitory activity. The process suggested in this work was suitable to obtain a potent ACE inhibitory activity product able to be incorporated into food formulas intended to control or lower blood pressure. Moreover, the liquid product could be easily stabilised by spray dried if it would be necessary.
authors.affiliation: Department of Chemical Engineering,University of Granada,18071 Granada,Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Espejo-Carpio
authors.affiliation: Department of Chemical Engineering,University of Granada,18071 Granada,Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Pérez-Gálvez
authors.affiliation: Department of Chemical Engineering,University of Granada,18071 Granada,Spain.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Almécija
authors.affiliation: Department of Chemical Engineering,University of Granada,18071 Granada,Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Guadix
authors.affiliation: Department of Chemical Engineering,University of Granada,18071 Granada,Spain.
authors.forename: Emilia M
authors.initials: EM
authors.lastname: Guadix
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000138:chemical synthesis*; D000818:Animals; D006041:Goats; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry*; D014462:Ultrafiltration / Q000379:methods / Q000662:veterinary*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D008894:Milk Proteins; D010447:Peptide Hydrolases