title: Magnetically guided recellularization of decellularized stented porcine pericardium-derived aortic valve for TAVI.
abstract: Application of somatic stem cells for growth, proliferation, and differentiation in a three-dimensional pattern is an important aspect in tissue engineering. Here, we report on our bioreactor, which we applied for magnetically guided recellularization of nitinol-stented valve. Human-derived unrestricted somatic stem cells were cultured in medium in our pulsatile dynamic bioreactor for 4-6 days. Stented valves were prepared by decellularization of porcine pericardium and construction of stented tissue-engineered valves (n = 8). A magnetic field was created around the bioreactor to prevent the loss of cells. In the control group, no magnetic device was used (n = 4). Morphological characterization was assessed by immunohistochemical staining of paraffin sections and electron microscopy. The bioreactor enabled the preservation of physiologic culture conditions with aerobic cell metabolism and physiological pH values. Histological analysis showed homogeneous seeding of the pericardium with progenitor cells in the recellularized samples, whereas no cell seeding could be observed in the nonmagnetic group. Our magnetically guided multifunctional bioreactor allows for an efficient three-dimensional culturing of somatic stem cells on decellularized organ-specific matrix.
authors.affiliation: From the *Department of Surgery, Heart and Vascular Institute, Milton S. Hershey, Medical Center, College of Medicine, Hershey; †Department of Cardiac Surgery, University of Heidelberg, Heidelberg, Germany; ‡Department of Pathology, The Methodist Hospital, Joan and Sanford I. Weill Medical College, Houston, Texas; §Department of Cardiac Surgery, University of Cologne, Cologne, Germany; ¶Department of Cardiac Surgery, The Methodist Hospital, Joan and Sanford I. Weill Medical College, Houston, Texas; and ‖Institute for Cell Therapeutics, Heinrich-Heine University Duesseldorf, Duesseldorf, Germany.
authors.forename: Ali
authors.initials: A
authors.lastname: Ghodsizad
authors.forename: Viktor
authors.initials: V
authors.lastname: Bordel
authors.forename: Herrman
authors.initials: H
authors.lastname: Wiedensohler
authors.forename: Ali
authors.initials: A
authors.lastname: Elbanayosy
authors.forename: Michael Martin
authors.initials: MM
authors.lastname: Koerner
authors.forename: Jose M
authors.initials: JM
authors.lastname: Gonzalez Berjon
authors.forename: Roberto
authors.initials: R
authors.lastname: Barrios
authors.forename: Mina
authors.initials: M
authors.lastname: Farag
authors.forename: Mohamad
authors.initials: M
authors.lastname: Zeriouh
authors.forename: Matthias
authors.initials: M
authors.lastname: Loebe
authors.forename: George Peter
authors.initials: GP
authors.lastname: Noon
authors.forename: Gesine
authors.initials: G
authors.lastname: Koegler
authors.forename: Matthias
authors.initials: M
authors.lastname: Karck
authors.forename: Arjang
authors.initials: A
authors.lastname: Ruhparwar
mesh_terms: D000818:Animals; D019149:Bioreactors; D002454:Cell Differentiation; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006351:Heart Valves / Q000166:cytology; D006801:Humans; D016130:Immunophenotyping; D060526:Magnetic Fields; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010496:Pericardium / Q000166:cytology*; D011673:Pulsatile Flow; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*