title: Lineage diversification of pigeon paramyxovirus effect re-emergence potential in chickens.
abstract: Genotype VI-paramyxovirus (GVI-PMV1) is a major cause of epidemic Newcastle-like disease in Columbiformes. This genotype of avian paramyxovirus type 1 has diversified rapidly since its introduction into the US in 1982 resulting in two extant lineages, which have different population growth properties. Although some GVI-PMV1s replicate poorly in chickens, it is possible that variants with different replicative or pathogenic potential in chickens exist among the genetically-diverse GVI-PMV1s strains. To determine if variants of Columbiform GVI-PMV1 with different phylogenetic affiliations have distinct phenotypic properties in chickens, we investigated the replicative properties of 10 naturally circulating pigeon-derived isolates representing four subgroups of GVI-PMV1 in primary chicken lung epithelial cells and in chicken embryos. Our data demonstrate that GVI-PMV1 variants have different infection phenotypes in their chicken source host and that properties reflect subgroup affiliation. These subgroup replicative properties are consistent with observed dynamics of viral population growth.
authors.affiliation: Center for Infectious Disease Dynamics, Department of Biology, The Pennsylvania State University, University Park, PA 16802, USA; Department of Zoology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia. Electronic address: yeelingchong@gmail.com.
authors.forename: Yee Ling
authors.initials: YL
authors.lastname: Chong
authors.affiliation: Center for Infectious Disease Dynamics, Department of Biology, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Oekyung
authors.initials: O
authors.lastname: Kim
authors.affiliation: Center for Infectious Disease Dynamics, Department of Biology, The Pennsylvania State University, University Park, PA 16802, USA. Electronic address: mposs@bx.psu.edu.
authors.forename: Mary
authors.initials: M
authors.lastname: Poss
mesh_terms: D000818:Animals; D018109:Avulavirus / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D045463:Avulavirus Infections / Q000662:veterinary*; D001715:Bird Diseases / Q000821:virology*; D002478:Cells, Cultured; D002645:Chickens / Q000821:virology*; D016000:Cluster Analysis; D010856:Columbidae / Q000821:virology*; D004847:Epithelial Cells / Q000821:virology; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D016019:Survival Analysis; D014481:United States; D014779:Virus Replication
chemical_list: D012367:RNA, Viral
keywords: Genetic-phenotypic association; Multivariate analysis; Paramyxovirus