title: Cultured autologous keratinocytes in the treatment of large and deep burns: a retrospective study over 15 years.
abstract: OBJECTIVE
  The aim was to review the use and indications of cultured autologous epidermis (CAE) in extensive burns and to evaluate the efficiency of our strategy of burn treatment.
  
  
  METHODS
  This retrospective study comprised 15 years (1997-2012).
  
  
  METHODS
  all patients who received CAE.
  
  
  METHODS
  patients who died before complete healing and patients who received exclusively cultured allogeneic keratinocytes. Evaluation criteria were clinical. Time and success of wound healing after CAE graft were evaluated.
  
  
  RESULTS
  A total of 63 patients were included with severity Baux score of 107 (from 70 to 140) and mean percentage of TBSA of 71% (from 40% to 97%). The CAE were used as Cuono method, in STSG donor sites and deep 2nd degree burns and in combination with large-meshed STSG (1:6-1:12) in extensively burned patients. Cuono method was used in 6 patients. The final take was 16% (0-30) because of the great fragility of the obtained epidermis. Nine patients with deep 2nd degree burns (mean TBSA 81%, from 60 to 97%) were successfully treated with only CAE without skin grafting. Combined technique (STSG meshed at 1:6-1:12 covered with CAE) was used in 27 patients (mean TBSA 69%, from 49% to 96%) with 85% success rate. Finally, donor sites treated with CAE in 49 patients could be harvested several times thanks to rapid epithelialization (time of wound healing was 7 days (from 5 to 10 days)).
  
  
  CONCLUSIONS
  The CAE allow rapid healing of STSG donor sites and deep 2nd second degree burns in extensively burned patients.
authors.affiliation: Tissues and Cells Bank, Edouard Herriot Hospital, Lyon, France.
authors.forename: Celine
authors.initials: C
authors.lastname: Auxenfans
authors.affiliation: Tissues and Cells Bank, Edouard Herriot Hospital, Lyon, France.
authors.forename: Veronique
authors.initials: V
authors.lastname: Menet
authors.affiliation: Burn Centre, Edouard Herriot Hospital, Lyon, France.
authors.forename: Zulma
authors.initials: Z
authors.lastname: Catherine
authors.affiliation: Burn Centre, Edouard Herriot Hospital, Lyon, France. Electronic address: cshipkov@gmail.com.
authors.forename: Hristo
authors.initials: H
authors.lastname: Shipkov
authors.affiliation: Burn Centre, Edouard Herriot Hospital, Lyon, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Lacroix
authors.affiliation: Burn Centre, Edouard Herriot Hospital, Lyon, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Bertin-Maghit
authors.affiliation: Tissues and Cells Bank, Edouard Herriot Hospital, Lyon, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Damour
authors.affiliation: Burn Centre, Edouard Herriot Hospital, Lyon, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Braye
mesh_terms: D000293:Adolescent; D000328:Adult; D002056:Burns / Q000601:surgery*; D018929:Cell Culture Techniques; D002478:Cells, Cultured / Q000637:transplantation*; D004817:Epidermis / Q000637:transplantation*; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D012189:Retrospective Studies; D016038:Skin Transplantation; D017768:Skin, Artificial; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014945:Wound Healing; D055815:Young Adult
keywords: Burns; Combined method; Cultured autologous epidermis; Cuono method; STSG donor sites; Second degree burns