title: The novel zinc cluster regulator Tog1 plays important roles in oleate utilization and oxidative stress response in Saccharomyces cerevisiae.
abstract: Many zinc cluster proteins have been shown to play a role in the transcriptional regulation of glucose-repressible genes during glucose exhaustion and diauxic shift. Here, we studied an additional member of this family called Yer184c (herein called Tog1) for transcriptional regulator of oleate. Our results showed that a Δtog1 strain displays impaired growth with several non-fermentable carbons. Tog1 is also implicated in oxidative stress tolerance. Importantly, during the glucose-oleate shift, combined results from quantitative real time-PCR and chromatin immunoprecipitation (ChIP) experiments showed that Tog1 acts as a direct activator of oleate utilizing genes, encoded key enzymes in β-Oxidation and NADPH regeneration (POX1, FOX2, POT1 and IDP2), the glyoxylate shunt (MLS1 and ICL1), and gluconeogenesis (PCK1 and FBP1). A transmission electron microscopy (TEM) analysis of the Δtog1 strain assayed with oleate also revealed a substantial decrease in peroxisome abundance that is vital for fatty acid oxidation. Overall, our results clearly demonstrated that Tog1 is a newly characterized zinc cluster regulator that functions in the complex network of non-fermentable carbon metabolism in Saccharomyces cerevisiae.
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tianthalay Road, Tha Kham, Bangkhuntian, Bangkok 10150, Thailand.
authors.forename: Piyasuda
authors.initials: P
authors.lastname: Thepnok
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tianthalay Road, Tha Kham, Bangkhuntian, Bangkok 10150, Thailand.
authors.forename: Khanok
authors.initials: K
authors.lastname: Ratanakhanokchai
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tianthalay Road, Tha Kham, Bangkhuntian, Bangkok 10150, Thailand. Electronic address: nitnipa.soo@kmutt.ac.th.
authors.forename: Nitnipa
authors.initials: N
authors.lastname: Soontorngun
mesh_terms: D001483:Base Sequence; D047369:Chromatin Immunoprecipitation; D017931:DNA Primers; D005800:Genes, Fungal; D046529:Microscopy, Electron, Transmission; D019301:Oleic Acid / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: D017931:DNA Primers; D029701:Saccharomyces cerevisiae Proteins; D019301:Oleic Acid
keywords: Carbon source utilization; Oleate induction; Oxidative stress; Saccharomyces cerevisiae; Zinc cluster protein