title: Polyphenols of selected peach and plum genotypes reduce cell viability and inhibit proliferation of breast cancer cells while not affecting normal cells.
abstract: Polyphenolic extracts and fractions of selected peach and plum genotypes were evaluated for cell viability and antiproliferation activity in vitro against an estrogen independent MDA-MB-435 and estrogen dependent MCF-7 breast cancer cell lines and one non-cancerous breast line MCF-10A. All extracts showed a phenolic dose-dependent cytotoxic effect against MDA-MB-435, weak activity against MCF-7 and small or no activity against MCF-10A. Genotype phenolic profiles showed varying degrees of polyphenolic mixtures. Fractionation of peach BY00P6653 extracts gave 4 fractions, with fraction F-I (caffeic acid derivatives) showing a strong activity against MDA-MB-435 followed by fraction F-II (anthocyanins). Induced-apoptosis by F-I on MDA-MB-435 was confirmed by Tunnel nuclear staining of cells with apoptotic DNA fragmentation (0-100 μg/mL) with no effects in normal cells (0-200 μg/mL). Selected stone fruit genotypes can be added to the list of fruits with cytotoxic effects against breast cancer cells while not affecting normal cells.
authors.affiliation: Embrapa Clima Temperado, BR 392, km 78, 96001-970 Pelotas, RS, Brazil(1); Dpt of Horticultural Sciences and Vegetable & Fruit Improvement Center, Texas A&M University, College Station, TX 77843, United States.
authors.forename: Marcia
authors.initials: M
authors.lastname: Vizzotto
authors.affiliation: Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, United States.
authors.forename: Weston
authors.initials: W
authors.lastname: Porter
authors.affiliation: Dpt of Horticultural Sciences and Vegetable & Fruit Improvement Center, Texas A&M University, College Station, TX 77843, United States.
authors.forename: David
authors.initials: D
authors.lastname: Byrne
authors.affiliation: Dpt of Horticultural Sciences and Vegetable & Fruit Improvement Center, Texas A&M University, College Station, TX 77843, United States. Electronic address: lcisnero@tamu.edu.
authors.forename: Luis
authors.initials: L
authors.lastname: Cisneros-Zevallos
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004967:Estrogens / Q000378:metabolism; D005260:Female; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D005838:Genotype; D006801:Humans; D020287:In Situ Nick-End Labeling; D061986:MCF-7 Cells; D042361:Mammary Glands, Human / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry* / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D004967:Estrogens; D010936:Plant Extracts; D059808:Polyphenols
keywords: Apoptosis; Breast cancer; Polyphenols; Prunus persica; Prunus salicina; Selective cytotoxicity