title: Vaccination with recombinant adenoviruses expressing the peste des petits ruminants virus F or H proteins overcomes viral immunosuppression and induces protective immunity against PPRV challenge in sheep.
abstract: Peste des petits ruminants (PPR) is a highly contagious disease of small ruminants caused by the Morbillivirus peste des petits ruminants virus (PPRV). Two recombinant replication-defective human adenoviruses serotype 5 (Ad5) expressing either the highly immunogenic fusion protein (F) or hemagglutinin protein (H) from PPRV were used to vaccinate sheep by intramuscular inoculation. Both recombinant adenovirus vaccines elicited PPRV-specific B- and T-cell responses. Thus, neutralizing antibodies were detected in sera from immunized sheep. In addition, we detected a significant antigen specific T-cell response in vaccinated sheep against two different PPRV strains, indicating that the vaccine induced heterologous T cell responses. Importantly, no clinical signs and undetectable virus shedding were observed after virulent PPRV challenge in vaccinated sheep. These vaccines also overcame the T cell immunosuppression induced by PPRV in control animals. The results indicate that these adenovirus constructs could be a promising alternative to current vaccine strategies for the development of PPRV DIVA vaccines.
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Rojas
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Madrid, Spain.
authors.forename: Héctor
authors.initials: H
authors.lastname: Moreno
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Madrid, Spain.
authors.forename: Félix
authors.initials: F
authors.lastname: Valcárcel
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Madrid, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Peña
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Madrid, Spain.
authors.forename: Noemí
authors.initials: N
authors.lastname: Sevilla
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Madrid, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Martín
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005260:Female; D029021:Peste-des-Petits-Ruminants / Q000276:immunology* / Q000517:prevention & control; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D012756:Sheep; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology / Q000627:therapeutic use
chemical_list: D014764:Viral Proteins; D014765:Viral Vaccines