title: Validation a solid-phase extraction-HPLC method for determining the migration behaviour of five aromatic amines from packaging bags into seafood simulants.
abstract: The simultaneous determination of five aromatic amines and their potential migration from packaging bags into seafood simulants were investigated. A validated HPLC method was developed for the separation and qualification of five aromatic amines in seafood simulants. By combining solid-phase extraction (SPE), these amines were efficiently separated on a Halo C18 column (150 × 4.6 mm i.d., 2.7 μm, particle size) using a mobile phase of methanol/phosphate buffer solution (5 mmol l(-1), pH 6.9) with gradient elution. The linear range was 0.1-10.0 mg l(-1); the absolute recoveries ranged from 85.3% to 98.4%; and the limits of detection of the five aromatic amines were between 0.015 and 0.08 mg l(-1). In this work the migration profile of aromatic amines from black plastic bags was investigated at temperatures of 4°C with water, 3% acetic acid solution, 10% ethanol solution and 50% ethanol solution as seafood simulants, respectively. The migration of the five aromatic amines under different conditions showed that residual o-methoxyaniline, p-chloroaniline, aniline and 2,6-dimethylaniline leaching from black plastic bags increased with incubation time. No detectable 3,3´-dimethylbenzidine was found to leach from the bags.
authors.affiliation: a School of Food and Pharmacy , Zhejiang Ocean University , Zhoushan , China.
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: OuYang
authors.forename: Yu-Yang
authors.initials: YY
authors.lastname: Luo
authors.forename: Yang-Guang
authors.initials: YG
authors.lastname: Wang
authors.forename: Li-Ye
authors.initials: LY
authors.lastname: Yang
mesh_terms: D000588:Amines / Q000737:chemistry*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D018857:Food Packaging; D006863:Hydrogen-Ion Concentration; D010969:Plastics / Q000737:chemistry; D015203:Reproducibility of Results; D017747:Seafood; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000588:Amines; D010969:Plastics
keywords: HPLC; aromatic amine; black plastic bag; migration; seafood simulant