title: Dark-field microscopy in imaging of plasmon resonant nanoparticles.
abstract: Dark-field microscopy (DFM) and spectroscopy base on localized surface plasmon resonance (LSPR) have been widely applied in biological sensing and single-molecule imaging. Using plasmonic nanoparticles with controlled geometrical, optical, and surface chemical properties as the probes, the scattering light depending on the surrounding environment can be detected by DF microscope. Signal-to-noise radio and time resolution of the conventional DFM is not sufficient to identify single molecular dynamics. To break these limitations, significant improvements have been made in recent years. This critical review is focused on the developments of the DFM and the utilization of DFM as a powerful technology in the application of LSPR detection.
authors.affiliation: Division of Physical Biology, and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Liu
authors.affiliation: Division of Physical Biology, and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chao
authors.affiliation: Division of Physical Biology, and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. Electronic address: dengsuhui@sinap.ac.cn.
authors.forename: Suhui
authors.initials: S
authors.lastname: Deng
authors.affiliation: Division of Physical Biology, and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Division of Physical Biology, and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.affiliation: Division of Physical Biology, and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
mesh_terms: D008853:Microscopy / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
keywords: Dark-field microscopy (DFM); Localized surface plasmon resonance (LSPR); Plasmonic nanoparticles