title: Defined single-gene and multi-gene deletion mutant collections in Salmonella enterica sv Typhimurium.
abstract: We constructed two collections of targeted single gene deletion (SGD) mutants and two collections of targeted multi-gene deletion (MGD) mutants in Salmonella enterica sv Typhimurium 14028s. The SGD mutant collections contain (1), 3517 mutants in which a single gene is replaced by a cassette containing a kanamycin resistance (KanR) gene oriented in the sense direction (SGD-K), and (2), 3376 mutants with a chloramphenicol resistance gene (CamR) oriented in the antisense direction (SGD-C). A combined total of 3773 individual genes were deleted across these SGD collections. The MGD collections contain mutants bearing deletions of contiguous regions of three or more genes and include (3), 198 mutants spanning 2543 genes replaced by a KanR cassette (MGD-K), and (4), 251 mutants spanning 2799 genes replaced by a CamR cassette (MGD-C). Overall, 3476 genes were deleted in at least one MGD collection. The collections with different antibiotic markers permit construction of all viable combinations of mutants in the same background. Together, the libraries allow hierarchical screening of MGDs for different phenotypic followed by screening of SGDs within the target MGD regions. The mutants of these collections are stored at BEI Resources (www.beiresources.org) and publicly available.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America.
authors.forename: Steffen
authors.initials: S
authors.lastname: Porwollik
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Santiviago
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America.
authors.forename: Pui
authors.initials: P
authors.lastname: Cheng
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America.
authors.forename: Fred
authors.initials: F
authors.lastname: Long
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America.
authors.forename: Prerak
authors.initials: P
authors.lastname: Desai
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Fredlund
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America.
authors.forename: Shabarinath
authors.initials: S
authors.lastname: Srikumar
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America; Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
authors.forename: Cecilia A
authors.initials: CA
authors.lastname: Silva
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America.
authors.forename: Weiping
authors.initials: W
authors.lastname: Chu
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America.
authors.forename: Rocío
authors.initials: R
authors.lastname: Canals
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: M Megan
authors.initials: MM
authors.lastname: Reynolds
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Lydia
authors.initials: L
authors.lastname: Bogomolnaya
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Christine
authors.initials: C
authors.lastname: Shields
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Ping
authors.initials: P
authors.lastname: Cui
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Jinbai
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Tiana
authors.initials: T
authors.lastname: Endicott-Yazdani
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Yang
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Aimee
authors.initials: A
authors.lastname: Maple
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Yury
authors.initials: Y
authors.lastname: Ragoza
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: Blondel
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
authors.forename: Camila
authors.initials: C
authors.lastname: Valenzuela
authors.affiliation: Department of Microbial Pathogenesis and Immunology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Helene
authors.initials: H
authors.lastname: Andrews-Polymenis
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: McClelland
mesh_terms: D002702:Chloramphenicol Resistance; D017353:Gene Deletion; D015723:Gene Library; D005798:Genes, Bacterial; D007613:Kanamycin Resistance; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics*; D017384:Sequence Deletion