title: Fabrication of mAb G250-SPIO molecular magnetic resonance imaging nanoprobe for the specific detection of renal cell carcinoma in vitro.
abstract: Molecular magnetic resonance imaging (mMRI) has been paid more and more attention for early diagnosis of cancer. A sensitive and specific mMRI probe plays the most important role in this technique. In this study, superparamagnetic iron oxide (SPIO) nanoparticles and mAb G250 were conjugated as mMRI probe for the detection of clear cell renal cell carcinoma (ccRCC) using 3.0-Tesla MRI in vitro. mAb G250 could specifically recognize carbonic anhydrase IX (CAIX) antigen overexpressed in ccRCC and the SPIO nanoparticles as MRI contrast agent presented excellent MRI response and good biocompatibility. The successful assembly of this nanoprobe was confirmed by UV-vis spectrum, FT-IR spectroscopy and DLS analysis. In vitro MRI study on ccRCC cells and control cells indicated that our fabricated mAb G250-SPIO nanoprobe could be used in the specific labeling of clear cell renal carcinoma cells successfully.
authors.affiliation: Department of Radiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, PR China.
authors.forename: Cailuan
authors.initials: C
authors.lastname: Lu
authors.affiliation: Department of Radiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, PR China; School of Medical Imaging, Xuzhou Medical College, Xuzhou, Jiangsu Province, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Radiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, PR China; School of Medical Imaging, Xuzhou Medical College, Xuzhou, Jiangsu Province, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Radiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Radiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, PR China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Radiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, PR China; School of Medical Imaging, Xuzhou Medical College, Xuzhou, Jiangsu Province, PR China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Han
authors.affiliation: Department of Radiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, PR China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002292:Carcinoma, Renal Cell / Q000175:diagnosis*; D045744:Cell Line, Tumor; D004355:Drug Stability; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D018796:Immunoconjugates / Q000378:metabolism; D007680:Kidney Neoplasms / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D059346:Magnets / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D005290:Ferric Compounds; C106533:G250 monoclonal antibody; D018796:Immunoconjugates; C000499:ferric oxide; D012710:Serum Albumin, Bovine