title: Dual effects of hydrogen sulfide donor on meiosis and cumulus expansion of porcine cumulus-oocyte complexes.
abstract: Hydrogen sulfide (H2S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H2S acts on the meiotic maturation of porcine oocytes, including key maturation factors such as MPF and MAPK, and cumulus expansion intensity of cumulus-oocyte complexes. We observed that the H2S donor, Na2S, accelerated oocyte in vitro maturation in a dose-dependent manner, following an increase of MPF activity around germinal vesicle breakdown. Concurrently, the H2S donor affected cumulus expansion, monitored by hyaluronic acid production. Our results suggest that the H2S donor influences oocyte maturation and thus also participates in the regulation of cumulus expansion. The exogenous H2S donor apparently affects key signal pathways of oocyte maturation and cumulus expansion, resulting in faster oocyte maturation with little need of cumulus expansion.
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Nevoral
authors.affiliation: Research Institute of Animal Production, Prague, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Petr
authors.affiliation: Laboratoire de Régulation des Signaux de Division - EA 4479, Université Lille1, Sciences et Technologies, IFR 147, Villeneuved'Ascq Cedex, France.
authors.forename: Armance
authors.initials: A
authors.lastname: Gelaude
authors.affiliation: Laboratoire de Régulation des Signaux de Division - EA 4479, Université Lille1, Sciences et Technologies, IFR 147, Villeneuved'Ascq Cedex, France.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Bodart
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Kucerova-Chrpova
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Sedmikova
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Krejcova
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Kolbabova
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Dvorakova
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Vyskocilova
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Ivona
authors.initials: I
authors.lastname: Weingartova
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Krivohlavkova
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Zalmanova
authors.affiliation: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Jilek
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D054885:Cumulus Cells / Q000166:cytology / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D064426:Gasotransmitters / Q000494:pharmacology*; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D016200:Maturation-Promoting Factor / Q000378:metabolism; D008540:Meiosis / Q000187:drug effects*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D013440:Sulfides / Q000494:pharmacology*; D013552:Swine
chemical_list: D064426:Gasotransmitters; D013440:Sulfides; D016200:Maturation-Promoting Factor; D048049:Extracellular Signal-Regulated MAP Kinases; C033479:sodium sulfide; D006862:Hydrogen Sulfide