title: Single amino acid arginine deprivation triggers prosurvival autophagic response in ovarian carcinoma SKOV3.
abstract: Autophagy is a process of cytosol-to-lysosome vesicle trafficking of cellular constituents for degradation and recycling of their building blocks. Autophagy becomes very important for cell viability under different stress conditions, in particular under amino acid limitation. In this report we demonstrate that single amino acid arginine deprivation triggers profound prosurvival autophagic response in cultured human ovarian cancer SKOV3 cells. In fact, a significant drop in viability of arginine-starved SKOV3 cells was observed when autophagy was inhibited by either coadministration of chloroquine or transcriptional silencing of the essential autophagy protein BECLIN 1. Enzymatic arginine deprivation is a novel anticancer therapy undergoing clinical trials. This therapy is considered nontoxic and selective, as it allows controlling the growth of malignant tumours deficient in arginine biosynthesis. We propose that arginine deprivation-based combinational treatments that include autophagy inhibitors (e.g., chloroquine) may produce a stronger anticancer effect as a second line therapy for a subset of chemoresistant ovarian cancers.
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street 14/16, Lviv 79005, Ukraine ; Laboratory of Molecular Pathology, Department of Health Sciences, Università del Piemonte Orientale, Via P. Solaroli 17, 28100 Novara, Italy.
authors.forename: Galyna
authors.identifier: 0000-0002-9633-6822
authors.initials: G
authors.lastname: Shuvayeva
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street 14/16, Lviv 79005, Ukraine.
authors.forename: Yaroslav
authors.identifier: 0000-0002-4183-4736
authors.initials: Y
authors.lastname: Bobak
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street 14/16, Lviv 79005, Ukraine.
authors.forename: Natalia
authors.initials: N
authors.lastname: Igumentseva
authors.affiliation: Laboratory of Molecular Pathology, Department of Health Sciences, Università del Piemonte Orientale, Via P. Solaroli 17, 28100 Novara, Italy.
authors.forename: Rossella
authors.identifier: 0000-0002-1528-331X
authors.initials: R
authors.lastname: Titone
authors.affiliation: Laboratory of Molecular Pathology, Department of Health Sciences, Università del Piemonte Orientale, Via P. Solaroli 17, 28100 Novara, Italy.
authors.forename: Federica
authors.identifier: 0000-0002-4903-6878
authors.initials: F
authors.lastname: Morani
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street 14/16, Lviv 79005, Ukraine.
authors.forename: Oleh
authors.initials: O
authors.lastname: Stasyk
authors.affiliation: Laboratory of Molecular Pathology, Department of Health Sciences, Università del Piemonte Orientale, Via P. Solaroli 17, 28100 Novara, Italy.
authors.forename: Ciro
authors.identifier: 0000-0002-5494-3034
authors.initials: C
authors.lastname: Isidoro
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001120:Arginine; D001343:Autophagy; D000071186:Beclin-1; D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D001120:Arginine