title: Characterization of oxygen transfer in a 24-well microbioreactor system and potential respirometric applications.
abstract: The assessment of microbial processes is often done in Microbioreactor systems (MBRs), which allow for parallel cultivation in multiple independent wells. MBRs often include dissolved oxygen sensors, which are convenient for process characterization through oxygen uptake rate and other respirometric determinations. In order to assess respirometric potential of MBRs, a complete assessment of the DO fluorescent quenching sensors was done, showing that they presented a typical error of 0.56%, a signal to noise ratio of 189, a response time from 5.7 to 7.2 s and no drift over a period of 24 h. Then, KLa in the MBR was measured with different cassette and cap designs, liquid volumes, agitation rates, gas flow rates, temperatures and ionic strengths. KLa ranged from 8 to 90 h(-1), with a standard deviation between replicates from 2.8 to 17.5%. From these results and a numerical simulation, it was shown that the MBR tested allow the determination of oxygen uptake rates in a range from 0.038 to 3390 mg L(-1) h(-1), with a determination error less than 15%. Besides OUR determination, it was concluded that the MBR tested is also a convenient tool for dynamic pulse respirometry methods, based on experimental confirmation with four different cultures.
authors.affiliation: Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Departamento de Biotecnología y Bioingeniería, México D.F., Mexico.
authors.forename: Rocio
authors.initials: R
authors.lastname: Ramirez-Vargas
authors.affiliation: Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Departamento de Biotecnología y Bioingeniería, México D.F., Mexico.
authors.forename: Miguel
authors.initials: M
authors.lastname: Vital-Jacome
authors.affiliation: Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Departamento de Física, México D.F., Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: Camacho-Perez
authors.affiliation: Pall Life Sciences, 25 Harbor Park Drive, Port Washington, NY 11050, USA.
authors.forename: Loe
authors.initials: L
authors.lastname: Hubbard
authors.affiliation: Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Departamento de Biotecnología y Bioingeniería, México D.F., Mexico. Electronic address: thalasso@cinvestav.mx.
authors.forename: Frederic
authors.initials: F
authors.lastname: Thalasso
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D003198:Computer Simulation; D005456:Fluorescent Dyes; D055616:Microtechnology / Q000295:instrumentation* / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D059629:Signal-To-Noise Ratio
chemical_list: D005456:Fluorescent Dyes; D010100:Oxygen
keywords: Micro-24; Microbioreactor; Microreactor; Pall; PreSens