title: Biosynthesis of silver nanoparticles using lingonberry and cranberry juices and their antimicrobial activity.
abstract: In this study lingonberry and cranberry juices were used for silver nanoparticle synthesis. The berry juices were characterized by total phenolics, total anthocyanins and benzoic acid content, respectively 1.9-2.7mg/ml, 55.2-83.4mg/l and 590.8-889.2mg/l. The synthesis of silver nanoparticles was performed at room temperature assisting in solutions irradiated by ultraviolet for 30min. Ultraviolet-visible (UV-vis) spectroscopy and microscopy confirmed the formation of nanoparticles as well as the dark red color of colloid of silver samples showed the formation of stable nanoparticles. Broad localized surface plasmon resonance (LSPR) peaks in UV-vis spectra indicated the formation of polydispersive silver nanoparticles and LSPR was observed at 485nm and 520nm for the silver nanoparticles synthesis using lingonberry and cranberry juices, respectively. The antimicrobial activity of silver nanoparticles was determined against the reference strains of microorganisms that could be found in food products: Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 13076, Listeria monocytogenes ATCC 19111, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231 and foodborne B. cereus producing and non-producing enterotoxins. Silver nanoparticles showed a broad spectrum of antimicrobial activity and were most active against S. aureus ATCC 25923, B. subtilis ATCC 6633 and B. cereus ATCC 11778 reference cultures, and less active against C. albicans ATCC 10231 and foodborne B. cereus. It can be concluded that lingonberry and cranberry juices could be used as bioreductants for silver ions.
authors.affiliation: Kaunas University of Technology, Department of Physics, Studentų str. 50, LT-51368 Kaunas, Lithuania.
authors.forename: Judita
authors.initials: J
authors.lastname: Puišo
authors.affiliation: Kaunas University of Technology, Food Institute, Taikos av. 92, LT-51180 Kaunas, Lithuania. Electronic address: dovile.jonkuviene@yahoo.com.
authors.forename: Dovilė
authors.initials: D
authors.lastname: Jonkuvienė
authors.affiliation: Kaunas University of Technology, Food Institute, Taikos av. 92, LT-51180 Kaunas, Lithuania.
authors.forename: Irena
authors.initials: I
authors.lastname: Mačionienė
authors.affiliation: Kaunas University of Technology, Food Institute, Taikos av. 92, LT-51180 Kaunas, Lithuania.
authors.forename: Joana
authors.initials: J
authors.lastname: Šalomskienė
authors.affiliation: Kaunas University of Technology, Department of Food Technology, Radvilėnų pl. 19, LT-50254 Kaunas, Lithuania.
authors.forename: Ina
authors.initials: I
authors.lastname: Jasutienė
authors.affiliation: State Research Institute Center for Physical Sciences and Technologies, Savanoriu av. 231, LT-02300 Vilnius, Lithuania.
authors.forename: Rokas
authors.initials: R
authors.lastname: Kondrotas
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001628:Beverages; D005658:Fungi / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D012834:Silver / Q000494:pharmacology*; D012996:Solutions; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet; D029799:Vaccinium macrocarpon / Q000737:chemistry*; D029801:Vaccinium vitis-idaea / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D012996:Solutions; D012834:Silver
keywords: Antimicrobial activity; Bacteria; Berry juices; Biosynthesis; Silver nanoparticles