title: Role of ubiquitin ligase Cbl-b in B-cell activation of dendritic cells loaded with Tα146-162 in the treatment of experimental autoimmune myasthenia gravis in mice.
abstract: OBJECTIVE
  This study aimed to examine the mechanism of Tα146-162-iMDC in the pathogenic intervention of mice with experimental autoimmune myasthenia gravis (EAMG) from the perspective of B-cell activation.
  
  
  METHODS
  The mice were divided into three groups, model (A), intervention (B), and control (C), with the intervention of Tα146-162-iMDC. The expressions of Cbl-b mRNA, Syk, Lyn, Btk, and phospholipase C (PLC)-γ2 proteins and their phosphorylated proteins were detected.
  
  
  RESULTS
  The Cbl-b mRNA expression in group A was lower than that in group C (p < 0.01) while that in group B increased compared with that in group A (p < 0.05), but was lower than that in group C (p < 0.05). The expression and phosphorylation of Syk and PLC-γ2 proteins in group A increased compared with those in group C (p < 0.01) while those in group B decreased compared with those in group A (p < 0.05), but were higher than those in group C (p < 0.05). The expression and phosphorylation of Lyn protein in group A decreased compared with those in group C (p < 0.01) while those in group B increased compared with those in group A (p < 0.05), but were lower than those in group C (p < 0.05). The Btk protein expression in group A increased compared with that in group C (p < 0.01) while that in group B decreased compared with that in group A (p < 0.05), but was still higher than that in group C (p < 0.05). However, no difference in phosphorylation levels among the three groups was observed (p > 0.05).
  
  
  CONCLUSIONS
  Tα146-162-iMDC intervention can reduce the incidence of EAMG and may be associated with Cbl-b in the negative regulation of B-cell activation.
authors.affiliation: Department of Geriatrics, the Second Xiangya Hospital, Central South University, Changsha, Hunan Province, ChinA. rongwangcn@163.com.
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009157:Myasthenia Gravis / Q000276:immunology* / Q000628:therapy*; D010446:Peptide Fragments / Q000276:immunology; D051966:Phospholipase C gamma / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000276:immunology; D013154:Spleen / Q000378:metabolism; D000072377:Syk Kinase; D014101:Torpedo; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000276:immunology*; D019061:src-Family Kinases / Q000378:metabolism
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D011976:Receptors, Muscarinic; D044767:Ubiquitin-Protein Ligases; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606897:Syk protein, mouse; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases; D051966:Phospholipase C gamma