title: Cellular barcoding: a technical appraisal.
abstract: Cellular barcoding involves the tagging of individual cells of interest with unique genetic heritable identifiers or barcodes and is emerging as a powerful tool to address individual cell fates on a large scale. However, as with many new technologies, diverse technical and analytical challenges have emerged. Here, we review those challenges and highlight both the power and limitations of cellular barcoding. We then illustrate the contribution of cellular barcoding to the understanding of hematopoiesis and outline the future potential of this technology.
authors.affiliation: Molecular Medicine Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia. Electronic address: shalinhnaik@gmail.com.
authors.forename: Shalin H
authors.initials: SH
authors.lastname: Naik
authors.affiliation: Division of Immunology, Netherlands Cancer Institute, Amsterdam, The Netherlands. Electronic address: t.schumacher@nki.nl.
authors.forename: Ton N
authors.initials: TN
authors.lastname: Schumacher
authors.affiliation: Division of Immunology, Netherlands Cancer Institute, Amsterdam, The Netherlands; Theoretical Biology and Bioinformatics, Utrecht University, Utrecht, The Netherlands. Electronic address: l.perie@nki.nl.
authors.forename: Leïla
authors.initials: L
authors.lastname: Perié
mesh_terms: D000818:Animals; D019070:Cell Lineage; D002469:Cell Separation; D058948:Cell Tracking / Q000379:methods*; D006410:Hematopoiesis; D006801:Humans