title: Disaccharidase activity in children with inflammatory bowel disease.
abstract: OBJECTIVE
  The etiopathogenesis of inflammatory bowel disease (IBD) is multifactorial and not well explained. Environmental, genetic, and dietary factors play an important role. The aim of the study was the evaluation of lactase, saccharase, and maltase activity in patients with IBD.
  
  
  METHODS
  The study comprised 65 children, aged 3-18 years. During a routine endoscopy, we took biopsies from the descending part of the duodenum. In these biopsies, we determined disaccharidase activity using Dahlquist's method.
  
  
  RESULTS
  Decreased lactase activity in the biopsies taken from the small intestine mucosa was most frequently observed in patients with Crohn's disease (5/15-33%) and least frequently seen in children with lymphocytic colitis (in 1/10-10%). The lowest mean values of lactase activity were found in the children with Crohn's disease and ulcerative colitis (1.7-2.5 U/1 g). Decreased saccharase activity in the biopsies obtained from the small intestine mucosa was most frequently observed in patients with lymphocytic colitis (in 5/10-50%) and ulcerative colitis (9/20-45%) and least frequently seen in children with non-specific undetermined colitis (in 7/20-35%). Decreased maltase activity in the small bowel mucosa was the most frequently observed in patients with Crohn's disease (in 5/15-33%) and least frequently seen in children with ulcerative colitis (in 3/20-15%). The lowest mean values of maltase activity were found in the children with Crohn's disease (5.4 U/1 g).
  
  
  CONCLUSIONS
  Therefore, it seems reasonable to perform diagnostic examinations aimed at lactose, saccharose, and maltose intolerance and to initiate a dietary regimen in children with IBD.
authors.affiliation: Department of Paediatrics, Medical University of Silesia, Katowice, Poland.
authors.forename: Sabina
authors.initials: S
authors.lastname: Wiecek
authors.forename: Halina
authors.initials: H
authors.lastname: Wos
authors.forename: Igor
authors.initials: I
authors.lastname: Radziewicz Winnicki
authors.forename: Marzena
authors.initials: M
authors.lastname: Komraus
authors.forename: Urszula
authors.initials: U
authors.lastname: Grzybowska Chlebowczyk
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D046730:Colitis, Lymphocytic / Q000201:enzymology / Q000473:pathology; D003093:Colitis, Ulcerative / Q000201:enzymology / Q000473:pathology; D003424:Crohn Disease / Q000201:enzymology / Q000473:pathology; D004384:Duodenoscopy; D004386:Duodenum / Q000201:enzymology; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000201:enzymology* / Q000473:pathology; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000473:pathology; D043322:Lactase / Q000378:metabolism*; D008297:Male; D012720:Severity of Illness Index; D000520:alpha-Glucosidases / Q000378:metabolism*; D043324:beta-Fructofuranosidase / Q000378:metabolism*
chemical_list: D043322:Lactase; D000520:alpha-Glucosidases; D043324:beta-Fructofuranosidase