title: Structural changes in halophilic and non-halophilic proteases in response to chaotropic reagents.
abstract: Halophilic enzymes have been established for their stability and catalytic abilities under harsh operational conditions. These have been documented to withstand denaturation at high temperature, pH, organic solvents, and chaotropic agents. However, this stability is modulated by salt. The present study targets an important aspect in understanding protein-urea/GdmCl interactions using proteases from halophilic Bacillus sp. EMB9 and non-halophilic subtilisin (Carlsberg) from Bacillus licheniformis as model systems. While, halophilic protease containing 1 % (w/v) NaCl (0.17 M) retained full activity towards urea (8 M), non-halophilic protease lost about 90 % activity under similar conditions. The secondary and tertiary structure were lost in non-halophilic but preserved for halophilic protein. This effect could be due to the possible charge screening and shielding of the protein surface by Ca(2+) and Na(+) ions rendering it stable against denaturation. The dialyzed halophilic protease almost behaved like the non-halophilic counterpart. Incorporation of NaCl (up to 5 %, w/v or 0.85 M) in dialyzed EMB9 protease containing urea/GdmCl, not only helped regain of proteolytic activity but also evaded denaturing action. Deciphering the basis of this salt modulated stability amidst a denaturing milieu will provide guidelines and templates for engineering stable proteins/enzymes for biotechnological applications.
authors.affiliation: Enzyme and Microbial Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110016, India.
authors.forename: Rajeshwari
authors.initials: R
authors.lastname: Sinha
authors.forename: S K
authors.initials: SK
authors.lastname: Khare
mesh_terms: D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry*; D004795:Enzyme Stability; D019791:Guanidine / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry*; D011489:Protein Denaturation; D012965:Sodium Chloride / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D012965:Sodium Chloride; D010447:Peptide Hydrolases; D019791:Guanidine