title: Glutaraldehyde cross-linking of immobilized thermophilic esterase on hydrophobic macroporous resin for application in poly(ε-caprolactone) synthesis.
abstract: The immobilized thermophilic esterase from Archaeoglobus fulgidus was successfully constructed through the glutaraldehyde-mediated covalent coupling after its physical adsorption on a hydrophobic macroporous resin, Sepabeads EC-OD. Through 0.05% glutaraldehyde treatment, the prevention of enzyme leaching and the maintenance of catalytic activity could be simultaneously realized. Using the enzymatic ring-opening polymerization of ε-caprolactone as a model, effects of organic solvents and reaction temperature on the monomer conversion and product molecular weight were systematically investigated. After the optimization of reaction conditions, products were obtained with 100% monomer conversion and Mn values lower than 1010 g/mol. Furthermore, the cross‑linked immobilized thermophilic esterase exhibited an excellent operational stability, with monomer conversion values exceeding 90% over the course of 12 batch reactions, still more than 80% after 16 batch reactions.
authors.affiliation: Department of Colorectal and Anal Surgery, The Second Hospital, Jilin University, Changchun 130041, China. wangmin20060513@hotmail.com.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. shihui13@mails.jlu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. Michaelwu_1990@live.cn.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. hanhb1310@mails.jlu.edu.cn.
authors.forename: Haobo
authors.initials: H
authors.lastname: Han
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. longde.guxiang@163.com.
authors.forename: Jianxu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. xz@163.com.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xing
authors.affiliation: Department of Dermatology, The Second Hospital, Jilin University, Changchun 130041, China. wmsshuang@hotmail.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. quanshun@jlu.edu.cn.
authors.forename: Quanshun
authors.initials: Q
authors.lastname: Li
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004950:Esterases / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D007700:Kinetics; D011091:Polyesters / Q000138:chemical synthesis*; D058105:Polymerization; D013696:Temperature
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D011091:Polyesters; C016240:polycaprolactone; D004950:Esterases; D005976:Glutaral