title: Thromboelastography in healthy dairy cows.
abstract: Thromboelastography is a whole blood-based coagulation assay that can be used to investigate hypocoagulability and hypercoagulability, as seen with thromboembolic diseases and disseminated intravascular coagulation. Numerous coagulopathies due to different causes are reported in cows. The objective was to establish reference intervals for thromboelastography using the TEG 5000 (Haemonetics GmbH, Munich, Germany) with citrated whole blood samples and kaolin activation in dairy cows and to investigate possible thromboelastographic changes between cows in different lactation periods. An additional objective was to test the stability of samples for up to 100h. Sixty blood samples from healthy Holstein-Friesian cows were examined. The samples were allocated to 3 different lactation groups (≤30 d postcalving, 31-99 d postcalving, ≥100 d postcalving). Thromboelastography was performed by using the TEG 5000 analyzer with citrated whole blood samples with kaolin activation. The calculated reference intervals were as follows: reaction time=2.2 to 6.2min, coagulation time=0.8 to 2.0min, angle α=58.2 to 81.8°, maximum amplitude=64.3 to 89.2mm, and clot rigidity=9.2 to 41.2 dyn/cm(2). The 3 different lactation groups showed no significant differences in TEG parameters. No significant difference was seen in samples stored for up to 48h at room temperature, which indicates that delays in processing samples, such as those arising during transit, are not an issue.
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom. Electronic address: Sommerey@laboklin.de.
authors.forename: Cora-Costanze
authors.initials: CC
authors.lastname: Sommerey
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.
authors.forename: Tim L
authors.initials: TL
authors.lastname: Williams
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.
authors.forename: Ian
authors.initials: I
authors.lastname: McCrone
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.
authors.forename: Aran
authors.initials: A
authors.lastname: Ruiz-Ferreras
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.
authors.forename: Dan
authors.initials: D
authors.lastname: Freeman
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.
authors.forename: Joy
authors.initials: J
authors.lastname: Archer
mesh_terms: D000818:Animals; D001681:Biological Assay; D001777:Blood Coagulation; D002417:Cattle; D019343:Citric Acid / Q000737:chemistry; D005260:Female; D005858:Germany; D007616:Kaolin / Q000494:pharmacology; D007774:Lactation; D012016:Reference Values; D013916:Thrombelastography / Q000662:veterinary*
chemical_list: D007616:Kaolin; D019343:Citric Acid
keywords: bovine; coagulation; lactation; reference interval; thromboelastography