title: Regulatory T cells inhibit microglia activation and protect against inflammatory injury in intracerebral hemorrhage.
abstract: Numerous evidence demonstrate that microglia mediated inflammatory injury plays a critical role in intracerebral hemorrhage (ICH). Therefore, the way to inhibit the inflammatory response is greatly needed. Treg cells have been shown to play a critical role in immunologic self-tolerance as well as anti-tumor immune responses and transplantation. In the current study, we transfered Treg cells in the ICH model, and investigated the effect. The cytokines of microglia were measured by ELISA, JNK/ERK and NF-κB were measured by Western blot and EMSA (Electrophoretic Mobility Shift Assay), animal behavior was evaluated by animal behavioristics. We found that Treg cells could inhibit microglia mediated inflammatory response through NF-κB activation via the JNK/ERK pathway in vitro, and improve neurological function in vivo. Our findings suggest that Treg cells could suppress inflammatory injury and represent a novel cell-based therapeutical strategy in ICH.
authors.affiliation: Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
authors.forename: Anyong
authors.initials: A
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Hanchao
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Chuangan
authors.initials: C
authors.lastname: Lin
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Shousen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The 476th Hospital of PLA, Fuzhou, Fujian 350025, China. Electronic address: ytt83812280@sina.com.
authors.forename: Bangqing
authors.initials: B
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002543:Cerebral Hemorrhage / Q000276:immunology* / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000276:immunology*; D016328:NF-kappa B / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D048049:Extracellular Signal-Regulated MAP Kinases; D048670:MAP Kinase Kinase 4; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse
keywords: Inflammatory injury; Intracerebral hemorrhage; Microglia; Regulatory T cells