title: Study of single nucleotide polymorphism (rs28368082) in SPO11 gene and its association with male infertility.
abstract: OBJECTIVE
  The present study is a case-control analysis of a SNP (rs28368082) in exon 7 of the SPO11 gene and its possible association with male infertility in three provinces of Iran. We also searched for genetic differences among populations.
  
  
  METHODS
  Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis, we genotyped 113 infertile men and 50 fertile controls. Then, samples consisting SNP, as determined by PCR-RFLP, were genotyped by sequencing. The differences in genotype distributions between cases and fertile controls were examined using Chi-squared analysis. The genetic difference between individuals with mutated nucleotide was investigated by phylogenetic trees. Genetic difference among populations (provinces) was analyzed through ANOVA test, and homogeneity was investigated using STRUCTURE and K-means clustering analysis.
  
  
  RESULTS
  According to the statistical analysis, the SNP was significantly associated with male infertility in all populations except oligozoospermic cases of the Center region. The phylogenetic trees showed partial genetic variation among the individuals, although ANOVA test showed no significant genetic difference between populations (provinces) for both azoospermic, and oligozoospermic cases. Eventually, we affirmed that individuals in the inclusive populations had genetic difference, but it was not statistically significant for dividing underlying populations to separate groups, so each population was homogenous.
  
  
  CONCLUSIONS
  Our study indicates that the mentioned polymorphism in SPO11 gene may be linked to the susceptibility of azoospermia and oligozoospermia male infertility in three provinces of Iran. Further studies are required to support obtained results. It finally should be noted that the possible association between a particular SNP and a specific disease completely depends on the underlying population.
authors.affiliation: Biology Department, Shahid Beheshti University, Tehran, Iran, e.ghalkhani@yahoo.com.
authors.forename: Esmat
authors.initials: E
authors.lastname: Ghalkhani
authors.forename: Masoud
authors.initials: M
authors.lastname: Sheidai
authors.forename: Hamid
authors.initials: H
authors.lastname: Gourabi
authors.forename: Zahra
authors.initials: Z
authors.lastname: Noormohammadi
authors.forename: Narjes
authors.initials: N
authors.lastname: Bakhtari
authors.forename: Ali Mohammad
authors.initials: AM
authors.lastname: Malekasgar
mesh_terms: D000704:Analysis of Variance; D004706:Endodeoxyribonucleases / Q000235:genetics*; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D060066:Protein Interaction Maps; D017422:Sequence Analysis, DNA; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D004706:Endodeoxyribonucleases; D000099257:Meiotic Recombination Protein SPO11