title: Effects and mechanism analysis of vascular endothelial growth factor and salvianolic acid B on 125I-low density lipoprotein permeability of the rabbit aortary endothelial cells.
abstract: Atherosclerosis is the common pathological basis of cardiovascular and cerebrovascular disease. This study aimed to investigate the effects of vascular endothelial growth factor (VEGF) and salvianolic acid B (SalB) on the permeability of the rabbit aortary endothelial cells (RAECs) and to figure out the possible underlying molecular mechanisms. The extravasation of (125)I-low density lipoprotein ((125)I-LDL) through the RAECs was significantly increased by VEGF and decreased by SalB. Meanwhile, the tight junction-associated proteins occludin and claudin-5 were found downregulated by VEGF and the caveolae structure proteins caveolin-1 and caveolin-2 upregulated, which were abolished by the infusion of SalB. In addition, a marked increase in levels of cGMP and protein kinase G-1 (PKG-1) as well as activation of nuclear factor-κB (NF-κB) p65 were found after VEGF infusion, which were attenuated by SalB. This study demonstrates that VEGF and SalB can alter the LDL permeability of the RAECs by a paracellular pathway (downregulation of occludin and claudin-5) and a transcellular pathway (upregulation of caveolin-1 and caveolin-2), in which the cGMP/PKG/NF-κB signal pathway is possibly involved. The experimental results provide a new method and basic knowledge of prevention and treatment for cardiovascular and cerebrovascular disease.
authors.affiliation: Emergency Department, Shanghai Tenth People's Hospital, Shanghai, 200072, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Ba
authors.forename: Hu
authors.initials: H
authors.lastname: Peng
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001572:Benzofurans / Q000008:administration & dosage*; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D008077:Lipoproteins, LDL / Q000493:pharmacokinetics*; D011817:Rabbits; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage*; D053630:Depsides
chemical_list: D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL; D019275:Radiopharmaceuticals; D042461:Vascular Endothelial Growth Factor A; C076944:salvianolic acid B; D053630:Depsides