title: Proteoglycan concentrations in healthy and diseased articular cartilage by Fourier transform infrared imaging and principal component regression.
abstract: Fourier transform infrared imaging (FTIRI) combining with principal component regression (PCR) analysis were used to determine the reduction of proteoglycan (PG) in articular cartilage after the transection of the anterior cruciate ligament (ACL). A number of canine knee cartilage sections were harvested from the meniscus-covered and meniscus-uncovered medial tibial locations from the control joints, the ACL joints at three time points after the surgery, and their contralateral joints. The PG loss in the ACL cartilage was related positively to the durations after the surgery. The PG loss in the contralateral knees was less than that of the ACL knees. The PG loss in the meniscus-covered cartilage was less than that of the meniscus-uncovered tissue in both ACL and contralateral knees. The quantitative mapping of PG loss could monitor the disease progression and repair processes in arthritis.
authors.affiliation: Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, PR China; Department of Physics and Center for Biomedical Research, Oakland University, Rochester, MI 48309, USA.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Physics and Center for Biomedical Research, Oakland University, Rochester, MI 48309, USA. Electronic address: xia@oakland.edu.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology*; D004285:Dogs; D005583:Fourier Analysis; D007719:Knee Joint / Q000473:pathology*; D010003:Osteoarthritis / Q000473:pathology*; D025341:Principal Component Analysis; D011509:Proteoglycans / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D011509:Proteoglycans
keywords: ACL-transection; Cartilage; FTIRI; Osteoarthritis; PCR; Proteoglycan