title: A fluorescent probe for site I binding and sensitive discrimination of HSA from BSA.
abstract: A fluorescent probe DH1 has been successfully developed to detect HSA via site I non-covalent bonding. DH1 shows a dramatic fluorescence enhancement towards HSA without interference from other proteins. The molecular docking method, for the first time, was utilized to provide deep insight into the sensing mechanism of the probe. Moreover, probe DH1 was successfully used to detect trace HSA in healthy human urine.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian 116024, P. R. China. fanjl@dlut.edu.cn.
authors.forename: Jiangli
authors.initials: J
authors.lastname: Fan
authors.forename: Wen
authors.initials: W
authors.lastname: Sun
authors.forename: Zhenkuan
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
authors.forename: Yueqing
authors.initials: Y
authors.lastname: Li
authors.forename: Jianfang
authors.initials: J
authors.lastname: Cao
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000737:chemistry*; D001665:Binding Sites; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D062105:Molecular Docking Simulation; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry*
chemical_list: D001578:Benzopyrans; D005456:Fluorescent Dyes; C533829:dicyanomethylene-4H-chromene; D012710:Serum Albumin, Bovine