title: Terrabacter koreensis sp. nov., isolated from soil of a flowerbed.
abstract: A Gram-staining-positive, non-spore-forming, rod-shaped, non-motile and aerobic bacterium, strain THG-e54(T), was isolated from soil of a flowerbed in Bucheon, South Korea. The taxonomic position of this bacterium was determined in an investigation based on a polyphasic approach. 16S rRNA gene sequence analysis revealed a clear affiliation of strain THG-e54(T) with the genus Terrabacter. Strain THG-e54(T) showed the closest phylogenetic relationship with Terrabacter tumescens DSM 20308(T) with a 16S rRNA gene sequence similarity of 98.6%. The G+C content of the genomic DNA was 71.0 mol%. The major cellular fatty acids of strain THG-e54(T) were iso-C15:0 and iso-C16:0, and the predominant menaquinone was menaquinone MK-8(H4). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified amino-containing phosphoglycolipid, unidentified phospholipids, an unidentified aminolipid and an unidentified aminophospholipid. The cell-wall peptidoglycan type was ll-diaminopimelic acid and the cell-wall sugars were glucose and ribose. DNA-DNA hybridization experiments revealed a low level of DNA-DNA relatedness (less than 35.2%) between strain THG-e54(T) and its five closest relatives. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain THG-e54(T) could be clearly distinguished from closely related recognized members of the genus Terrabacter. Therefore the results of this study indicated the existence of a novel species of the genus Terrabacter , for which we propose the name Terrabacter koreensis sp. nov., with strain THG-e54(T) ( = KACC 17589(T) = JCM 19342(T)) as the type strain.
authors.affiliation: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: KyungHwa
authors.initials: K
authors.lastname: Won
authors.affiliation: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors.forename: MooChang
authors.initials: M
authors.lastname: Kook
authors.affiliation: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8