title: Quantitative assessment of rest and acetazolamide CBF using quantitative SPECT reconstruction and sequential administration of (123)I-iodoamphetamine: comparison among data acquired at three institutions.
abstract: OBJECTIVE
  A recently developed technique which reconstructs quantitative images from original projection data acquired using existing single-photon emission computed tomography (SPECT) devices enabled quantitative assessment of cerebral blood flow (CBF) at rest and after acetazolamide challenge. This study was intended to generate a normal database and to investigate its inter-institutional consistency.
  
  
  METHODS
  The three institutions carried out a series of SPECT scanning on 32 healthy volunteers, following a recently proposed method that involved dual administration of (123)I-iodoamphetamine during a single SPECT scan. Intra-institute and inter-institutional variations of regional CBF values were evaluated both at rest and after acetazolamide challenge. Functional images were pooled for both rest and acetazolamide CBF, and inter-institutional difference was evaluated among these images using two independent software programs.
  
  
  RESULTS
  Quantitative assessment of CBF images at rest and after acetazolamide was successfully achieved with the given protocol in all institutions. Intra-institutional variation of CBF values at rest and after acetazolamide was consistent with previously reported values. Quantitative CBF values showed no significant difference among institutions in all regions, except for a posterior cerebral artery region after acetazolamide challenge in one institution which employed SPECT device with lowest spatial resolution. Pooled CBF images at rest and after acetazolamide generated using two software programs showed no institutional differences after equalization of the spatial resolution.
  
  
  CONCLUSIONS
  SPECT can provide reproducible images from projection data acquired using different SPECT devices. A common database acquired at different institutions may be shared among institutions, if images are reconstructed using a quantitative reconstruction program, and acquired by following a standardized protocol.
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka, 565-8565, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Yamauchi
authors.forename: Etsuko
authors.initials: E
authors.lastname: Imabayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsuda
authors.forename: Jyoji
authors.initials: J
authors.lastname: Nakagawara
authors.forename: Masaaki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Eku
authors.initials: E
authors.lastname: Shimosegawa
authors.forename: Jun
authors.initials: J
authors.lastname: Hatazawa
authors.forename: Michiyasu
authors.initials: M
authors.lastname: Suzuki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Iwanaga
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukuda
authors.forename: Koji
authors.initials: K
authors.lastname: Iihara
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Iida
mesh_terms: D000086:Acetazolamide / Q000494:pharmacology*; D000368:Aged; D000662:Amphetamines; D001921:Brain / Q000098:blood supply / Q000000981:diagnostic imaging* / Q000187:drug effects* / Q000502:physiology; D001931:Brain Mapping; D002317:Cardiovascular Agents / Q000494:pharmacology*; D002560:Cerebrovascular Circulation / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007457:Iodine Radioisotopes; D008297:Male; D008875:Middle Aged; D019275:Radiopharmaceuticals; D012146:Rest; D012984:Software; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
chemical_list: D000662:Amphetamines; D002317:Cardiovascular Agents; D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D000086:Acetazolamide