title: Study of curcumin immunomodulatory effects on reactive astrocyte cell function.
abstract: Multiple sclerosis (MS) is considered an inflammatory and neurodegenerative disease of the central nervous system (CNS) which most often presents as relapsing-remitting episodes. Recent evidence suggests that activated astrocytes play a dual functional role in CNS inflammatory disorders such as MS. In this study, we tried to induce anti-inflammatory functions of astrocytes by curcumin. The effects of curcumin were examined on human a astrocyte cell line (U373-MG) induced by lipopolysaccharide (LPS) in vitro. Matrix metalloproteinase (MMP)-9 activity was assessed by gelatin zymography. Cytokine levels were evaluated by quantitative ELISA method and mRNA expression was measured by real-time PCR. We found that curcumin decreased the release of IL-6 and reduced MMP-9 enzyme activity. It down-regulated MCP-1 mRNA expression too. However, curcumin did not have significant effects on the expression of neurotrophin (NT)-3 and insulin-like growth factor (IGF)-1 mRNAs. Results suggest that curcumin might beneficially affect astrocyte population in CNS neuroinflammatory environment lean to anti-inflammatory response and help to components in respects of CNS repair. Our findings offer curcumin as a new therapeutic agent with the potential of regulating astrocyte-mediated inflammatory diseases in the CNS.
authors.affiliation: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Immunology Asthma & Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: seyedzadeh@razi.tums.ac.ir.
authors.forename: Mir Hadi
authors.initials: MH
authors.lastname: Seyedzadeh
authors.affiliation: Immunology Asthma & Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: zohreh_safari67@yahoo.com.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Safari
authors.affiliation: Immunology Asthma & Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: ahadzr@gmail.com.
authors.forename: Ahad
authors.initials: A
authors.lastname: Zare
authors.affiliation: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: jamshid.gholizadeh@live.com.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Gholizadeh Navashenaq
authors.affiliation: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: razavial@tums.ac.ir.
authors.forename: Seyed Alireza
authors.initials: SA
authors.lastname: Razavi
authors.affiliation: Immunology Asthma & Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: gakardar@tums.ac.ir.
authors.forename: Gholam Ali
authors.initials: GA
authors.lastname: Kardar
authors.affiliation: Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Biosensor Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: khoramza@tums.ac.ir.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Khorramizadeh
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001253:Astrocytes / Q000276:immunology*; D002460:Cell Line; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D003474:Curcumin / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D056747:Immunomodulation; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000276:immunology; D011134:Polysaccharides / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D015850:Interleukin-6; D008070:Lipopolysaccharides; D011134:Polysaccharides; C010491:neurotropin; D007334:Insulin-Like Growth Factor I; D020780:Matrix Metalloproteinase 9; D003474:Curcumin
keywords: Astrocyte; Curcumin; Immunomodulatory; Multiple sclerosis