title: Evaluation of metabolic enzymes in response to Excel Mera 71, a glyphosate-based herbicide, and recovery pattern in freshwater teleostean fishes.
abstract: Metabolic enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were evaluated in Indian teleostean fishes, namely, Anabas testudineus (Bloch) and Heteropneustes fossilis (Bloch), for an exposure to 30 days of Excel Mera 71 (17.2 mg/L), a glyphosate formulation, and subsequent depuration under Liv.52, a plant extract at a dose of 187.5 mg/d/250 L for the same period in the same tissues under laboratory condition. ALT activity was significantly increased (P<0.05) in all the tissues and raised up to 229.19% in liver of A. testudineus (229.19%) and 128.61% in liver of H. fossilis. AST also increased significantly (P<0.05) and was maximum in liver of H. fossilis (526.19%) and minimum in gill of A. testudineus (124.38%). ALP activity was also raised highly in intestine of H. fossilis (490.61%) but was less in kidney of H. fossilis (149.48%). The results indicated that Excel Mera 71 caused alterations in the metabolic enzymatic activities in fish tissues and AST showed the highest alteration in both the fishes, while lowest in ALP and ALT in A. testudineus and H. fossilis, respectively. During depuration under Liv.52, all the enzyme activities came down towards the control condition which indicated the compensatory response by the fish against this herbicidal stress and it was in the following order: AST>ALT>ALP, in A. testudineus, while H. fossilis showed the following trend: ALT>AST>ALP. Therefore, these parameters could be used as indicators of herbicidal pollution in aquatic organisms and were recommended for environmental monitoring for investigating the mechanism involved in the recovery pattern.
authors.affiliation: Ecotoxicology Laboratory, Department of Environmental Science, The University of Burdwan, Golapbag, Burdwan, West Bengal 713104, India.
authors.forename: Palas
authors.initials: P
authors.lastname: Samanta
authors.affiliation: Department of Environmental Science, Aghorekamini Prakashchandra Mahavidyalaya, Subhasnagar, Bengai, Hooghly, West Bengal 712611, India.
authors.forename: Sandipan
authors.initials: S
authors.lastname: Pal
authors.affiliation: P.G. Department of Conservation Biology, Durgapur Government College, Durgapur, West Bengal 713214, India.
authors.forename: Aloke Kumar
authors.initials: AK
authors.lastname: Mukherjee
authors.affiliation: Ecotoxicology Laboratory, Department of Environmental Science, The University of Burdwan, Golapbag, Burdwan, West Bengal 713104, India.
authors.forename: Apurba Ratan
authors.initials: AR
authors.lastname: Ghosh
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D004784:Environmental Monitoring; D005399:Fishes / Q000378:metabolism; D005618:Fresh Water; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D006540:Herbicides / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D009132:Muscles / Q000187:drug effects / Q000201:enzymology
chemical_list: D006540:Herbicides; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase