title: Laser speckle contrast imaging for assessment of abdominal visceral microcirculation in acute peritonitis: does sequential impairments exist?
abstract: OBJECTIVE
  It is believed that the microcirculation of multiple organs is impaired during acute peritonitis, however whether distinct susceptibilities of visceral microvasculature exist is still unknown. The present study aims to verify whether the microcirculatory alterations occur sequentially among multiple abdominal viscera during acute peritonitis.
  
  
  METHODS
  Acute peritonitis was achieved on 29 Sprague-Dawley rats through colon ascendens stent peritonitis (CASP) model. With laser speckle contrast imaging (LSCI), the microcirculation of the liver, ileum and renal cortex was monitored in each rat at baseline before CASP sepsis and continued monitoring at 4h, 8h, or 12h after the surgery. Another 9 rats served for sham operation. One-way analysis of variance with a post hoc Dunnett's test was used for analysis.
  
  
  RESULTS
  The ileum microcirculation was impaired earliest from 342.1±61.0 laser speckle perfusion unit (LSPU) at baseline to 271.7±74.0 LSPU at 4h (P<0.05), while the decline of renal microcirculation was not obvious until 8h after peritonitis (289.1±111.2 vs 376.2±53.4, P<0.05). However hepatic microcirculation was not significantly changed during 12h of observation period.
  
  
  CONCLUSIONS
  The microcirculation of various viscera has shown distinct susceptibilities to acute peritonitis: the ileum is more susceptible than the kidney, while the hepatic microcirculation seems to be the most resistant to peritonitis.
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, China. Electronic address: stephending007@gmail.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Ding
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, China. Electronic address: jiananR@gmail.com.
authors.forename: Jianan
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, China. Electronic address: zhb0468@gmail.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, China. Electronic address: cathywuyin@126.com.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Binjiang College, Zhejiang Chinese Medical University, China. Electronic address: 13185097375@163.com.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Shao
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, China. Electronic address: Wgfwang@gmail.com.
authors.forename: Gefei
authors.initials: G
authors.lastname: Wang
authors.affiliation: Binjiang College, Zhejiang Chinese Medical University, China. Electronic address: fangjianqiao7532@163.com.
authors.forename: Jianqiao
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, China. Electronic address: ljsurgeon@163.com.
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
mesh_terms: D000208:Acute Disease; D000818:Animals; D001783:Blood Flow Velocity; D004195:Disease Models, Animal; D007082:Ileum / Q000098:blood supply*; D007672:Kidney Cortex / Q000098:blood supply*; D017078:Laser-Doppler Flowmetry; D008102:Liver Circulation; D008297:Male; D008833:Microcirculation; D010538:Peritonitis / Q000175:diagnosis* / Q000503:physiopathology; D011237:Predictive Value of Tests; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation; D013152:Splanchnic Circulation; D013997:Time Factors
keywords: Acute peritonitis; Direct monitor; Distinct susceptibilities; Hepatic Microcirculation; Ileum mirculation; Laser speckle contrast imaging; Microcirculation alteration; Sepsis; Sequential impairments; Visceral vasculature