title: PEDF and PEDF-derived peptide 44mer protect cardiomyocytes against hypoxia-induced apoptosis and necroptosis via anti-oxidative effect.
abstract: Pigment epithelium-derived factor (PEDF) has many biological activities. But it's not known whether PEDF and its functional peptides could protect against hypoxia-induced cell death and the mechanisms are still unclear. We used cultured H9c2 cells and primary cardiomyocytes to show that apoptosis and necroptosis were significantly increased after hypoxia. Both PEDF and its fuctional peptides 44mer reduced apoptosis and necroptosis rates and inhibited the expression of cleaved caspase 3 and receptor-interacting protein 3 (RIP3). Furthermore, PEDF and 44mer could up-regulate super oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) levels, promote clearing of reactive oxygen species (ROS) and malondialdehyde (MDA). While, 34mer, another functional peptides had no effect on cell apoptosis and necroptosis. Hereby this is the first evidence that PEDF and its functional peptide 44mer protect cultured H9c2 cells and primary cardiomyocytes against apoptosis and necroptosis under hypoxic condition via the anti-oxidative mechanism.
authors.affiliation: 1] Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou, Jiangsu, China 221002 [2].
authors.forename: Xiao
authors.initials: X
authors.lastname: Gao
authors.affiliation: 1] Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou, Jiangsu, China 221002 [2].
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: 1] Center of Neurobiological Research, 209 Tongshan Road, Xuzhou Medical College, Xuzhou, Jiangsu, China 221004 [2].
authors.forename: Wei
authors.initials: W
authors.lastname: Zhuang
authors.affiliation: Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou, Jiangsu, China 221002.
authors.forename: Guangda
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou, Jiangsu, China 221002.
authors.forename: Teng
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou, Jiangsu, China 221002.
authors.forename: Xia
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou, Jiangsu, China 221002.
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Center of Neurobiological Research, 209 Tongshan Road, Xuzhou Medical College, Xuzhou, Jiangsu, China 221004.
authors.forename: Honghua
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou, Jiangsu, China 221002.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Center of Neurobiological Research, 209 Tongshan Road, Xuzhou Medical College, Xuzhou, Jiangsu, China 221004.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Dong
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D005136:Eye Proteins / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D009336:Necrosis / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D053422:Receptor-Interacting Protein Serine-Threonine Kinases / Q000378:metabolism; D015843:Serpins / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D000975:Antioxidants; D053148:Caspase 3; D005136:Eye Proteins; D005979:Glutathione Peroxidase; D009414:Nerve Growth Factors; D010455:Peptides; D017382:Reactive Oxygen Species; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; D015843:Serpins; D013482:Superoxide Dismutase; D000099174:Pigment Epithelium-Derived Factor; C582791:Ripk3 protein, rat