title: High-resolution structure of a virally encoded DNA-translocating conduit and the mechanism of DNA penetration.
abstract: Although ϕX174 DNA pilot protein H is monomeric during procapsid assembly, it forms an oligomeric tube on the host cell surface. Reminiscent of a double-stranded DNA phage tail in form and function, the H tube transports the single-stranded ϕX174 genome across the Escherichia coli cell wall. The 2.4-Å resolution H-tube crystal structure suggests functional and energetic mechanisms that may be common features of DNA transport through virally encoded conduits.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA bfane@email.arizona.edu mr@purdue.edu.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Rossmann
authors.affiliation: The BIO5 Institute, University of Arizona, Tucson, Arizona, USA bfane@email.arizona.edu mr@purdue.edu.
authors.forename: Bentley A
authors.initials: BA
authors.lastname: Fane
mesh_terms: D010584:Bacteriophage phi X 174 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000821:virology*; D008958:Models, Molecular; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins