title: Uniaxial cyclic strain enhances adipose-derived stem cell fusion with skeletal myocytes.
abstract: Although adult muscle tissue possesses an exceptional capacity for regeneration, in the case of large defects, the restoration to original state is not possible. A well-known source for the de novo regeneration is the adipose-derived stem cells (ASCs), which can be readily isolated and have been shown to have a broad differentiation and regenerative potential. In this work, we employed uniaxial cyclic tensile strain (CTS), to mechanically stimulate human ASCs to participate in the formation skeletal myotubes in an in vitro model of myogenesis. The application of CTS for 48h resulted in the formation of a highly ordered array of parallel ASCs, but failed to support skeletal muscle terminal differentiation. When the same stimulation paradigm was applied to cocultures with mouse skeletal muscle myoblasts, the percentage of ASCs contributing to the formation of myotubes significantly exceeded the levels reported in the literature hitherto. In perspective, the mechanical strain may be used to increase the efficiency of incorporation of ASCs in the skeletal muscles, which could be found useful in diverse traumatic or pathologic scenarios.
authors.affiliation: Laboratory for Stem Cell Research, Dept. of Health Science and Technology, Aalborg University, Denmark.
authors.forename: Jens Isak
authors.initials: JI
authors.lastname: Andersen
authors.affiliation: Laboratory for Stem Cell Research, Dept. of Health Science and Technology, Aalborg University, Denmark.
authors.forename: Morten
authors.initials: M
authors.lastname: Juhl
authors.affiliation: Laboratory for Stem Cell Research, Dept. of Health Science and Technology, Aalborg University, Denmark.
authors.forename: Thøger
authors.initials: T
authors.lastname: Nielsen
authors.affiliation: Laboratory for Stem Cell Research, Dept. of Health Science and Technology, Aalborg University, Denmark.
authors.forename: Jeppe
authors.initials: J
authors.lastname: Emmersen
authors.affiliation: Laboratory for Stem Cell Research, Dept. of Health Science and Technology, Aalborg University, Denmark.
authors.forename: Trine
authors.initials: T
authors.lastname: Fink
authors.affiliation: Laboratory for Stem Cell Research, Dept. of Health Science and Technology, Aalborg University, Denmark.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zachar
authors.affiliation: Laboratory for Stem Cell Research, Dept. of Health Science and Technology, Aalborg University, Denmark. Electronic address: cpennisi@hst.aau.dk.
authors.forename: Cristian Pablo
authors.initials: CP
authors.lastname: Pennisi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D018920:Coculture Techniques; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D032446:Myoblasts / Q000166:cytology / Q000502:physiology; D013314:Stress, Mechanical
keywords: Adipose-derived stem cells; C2C12; Cell fusion; Coculture; Myogenic differentiation; Uniaxial cyclic strain