title: Cell reprogramming into the pluripotent state using graphene based substrates.
abstract: Graphene has been attracting considerable interest in the field of biomedical engineering because graphene and its derivatives are considered to be ideal platforms for supporting cell growth and differentiation. Here we report that graphene promotes the reprogramming of mouse somatic fibroblasts into induced pluripotent stem cells (iPSCs). We constructed a layer of graphene film on a glass substrate and characterized it as a monolayer using Raman spectroscopy. We found that the graphene substrate significantly improved cellular reprogramming efficiency by inducing mesenchymal-to-epithelial-transition (MET) which is known to affect H3K4me3 levels. Thus, our results reveal that a graphene substrate directly regulates dynamic epigenetic changes associated with reprogramming, providing an efficient tool for epigenetic pluripotent reprogramming.
authors.affiliation: Laboratory of Stem Cells and Cell Reprogramming, Department of Biomedical Engineering, Dongguk University, Seoul 100-715, Republic of Korea.
authors.forename: Junsang
authors.initials: J
authors.lastname: Yoo
authors.affiliation: Division of Physics and Semiconductor Science, Dongguk University, Seoul 100-715, Republic of Korea.
authors.forename: Jongmin
authors.initials: J
authors.lastname: Kim
authors.affiliation: Laboratory of Stem Cells and Cell Reprogramming, Department of Biomedical Engineering, Dongguk University, Seoul 100-715, Republic of Korea.
authors.forename: Soonbong
authors.initials: S
authors.lastname: Baek
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan 689-798, Republic of Korea.
authors.forename: Youngsin
authors.initials: Y
authors.lastname: Park
authors.affiliation: Division of Physics and Semiconductor Science, Dongguk University, Seoul 100-715, Republic of Korea.
authors.forename: Hyunsik
authors.initials: H
authors.lastname: Im
authors.affiliation: Laboratory of Stem Cells and Cell Reprogramming, Department of Biomedical Engineering, Dongguk University, Seoul 100-715, Republic of Korea. Electronic address: jk2316@gmail.com.
authors.forename: Jongpil
authors.initials: J
authors.lastname: Kim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming / Q000187:drug effects*; D044127:Epigenesis, Genetic; D058750:Epithelial-Mesenchymal Transition; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006108:Graphite / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID
chemical_list: D001672:Biocompatible Materials; D006108:Graphite
keywords: Graphene; Induced pluripotent stem cells; Mesenchymal-to-epithelial transition; Reprogramming