title: Cholesteryl-modification of a glucomannan from Bletilla striata and its hydrogel properties.
abstract: A glucomannan-type polysaccharide, named BSP, was obtained from the tubers of Bletilla striata by ultrasonic-assisted extraction, ethanol precipitation, deproteination and gel-permeation chromatography. HPLC analysis revealed that BSP contained mannose and glucose in the molar ratio of 3.5:1. Its molecular weight (Mw) was estimated to be 20 kDa. Methylation analysis, FT-IR and NMR analyses indicated that BSP consisted of (1→4)-linked β-D-glucopyranosyl residues and (1→4)-linked β-D-mannopyranosyl residues. Cholesteryl succinate was linked to BSP to make it more amphiphilic and the degree of substitution of cholesteryl succinate-BSP was 3.2%. The critical micelle concentration of modified BSP was 0.001 mg/mL, suggesting it could self-assemble into nanoparticles in aqueous solution.
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, China. zhangms253@nenu.edu.cn.
authors.forename: Mengshan
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, China. sunl925@nenu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.affiliation: Pharmacy Department, Chinese People's Liberation Army 208 Hospital, Changchun 130062, China. zhaowencui208@126.com.
authors.forename: Wencui
authors.initials: W
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, China. pengxx039@nenu.edu.cn.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Peng
authors.affiliation: Pharmacy Department, Chinese People's Liberation Army 208 Hospital, Changchun 130062, China. cclfq@126.com.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Pharmacy Department, Chinese People's Liberation Army 208 Hospital, Changchun 130062, China. aswangyanping@163.com.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Pharmacy Department, Chinese People's Liberation Army 208 Hospital, Changchun 130062, China. asbiyajing@163.com.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Pharmacy Department, Chinese People's Liberation Army 208 Hospital, Changchun 130062, China. zqi8229@126.com.
authors.forename: Hengbi
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, China. zhouyf383@nenu.edu.cn.
authors.forename: Yifa
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002788:Cholesterol Esters / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008745:Methylation; D008823:Micelles; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D035281:Plant Tubers / Q000737:chemistry
chemical_list: D002788:Cholesterol Esters; D020100:Hydrogels; D008351:Mannans; D008823:Micelles; D010936:Plant Extracts