title: Comparative RNA-sequencing analysis of mthl1 functions and signal transductions in Tribolium castaneum.
abstract: Methuselah-like 1 of Tribolium castaneum (TcMthl1) has been reported to play crucial roles in development, lifespan, stress resistance and reproduction. However, the signaling system of TcMthl1 is unknown. Thus, we compare the transcriptome profile of RNAi treated larvae (ds-Tcmthl1) and control larvae of T. castaneum by RNA-sequencing, and obtained 14,613,514 sequence reads aligned with 13,533 genes; 812 differentially expressed genes (DEGs) were identified. These DEGs were classified into 47 GO functional groups, including such functions as the immune system process, the response to stimulus, the developmental process and reproduction. Interestingly, knock-down of Tcmthl1 suppressed both of Toll and IMD immunity pathways which most likely modulated the effects of Tcmthl1 on lifespan and stress resistance. Additionally, the DEGs encoding Blimp-1, Ftz-F1, E74 and Timeless may participate in the development and reproduction of ds-Tcmthl1 insects. The findings of these DEGs and pathways will provide valuable insight into TcMthl1 signaling and regulation system.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address: lcj3314@163.com.
authors.forename: Chengjun
authors.initials: C
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address: ztaiww1314@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address: sangming1105@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Sang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address: mexing@163.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address: whhuxingxing@163.com.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Hu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address: yunxiaopei619@126.com.
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Yun
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address: libin@njnu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D015398:Signal Transduction / Q000235:genetics*; D059467:Transcriptome; D014231:Tribolium / Q000254:growth & development / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D043562:Receptors, G-Protein-Coupled
keywords: IMD; Immune; Methuselah; RNA-sequencing; Toll; Tribolium castaneum