title: DNase 1 retains endodeoxyribonuclease activity following gold nanocluster synthesis.
abstract: Here we present the synthesis of the enzyme DNase 1 stabilized gold nanoclusters (DNase 1:AuNCs) with core size consisting of either 8 or 25 atoms. The DNase 1:Au8NCs exhibit blue fluorescence whereas the DNase 1:Au25NCs are red emitting. In addition to the intense fluorescence emission, the synthesized DNase 1:AuNC hybrid retains the native functionality of the protein, allowing simultaneous detection and digestion of DNA with a detection limit of 2 μg/mL. The DNase 1:AuNCs could be conveniently employed as efficient and fast sensors to augment the current time-consuming DNA contamination analysis techniques.
authors.affiliation: Weapons and Materials Research Directorate, U.S. Army Research Laboratory , ATTN: RDRL-WM, 4600 Deer Creek Loop, Aberdeen Proving Ground, Aberdeen, Maryland 21005-5069, United States.
authors.forename: Abby L
authors.initials: AL
authors.lastname: West
authors.forename: Mark H
authors.initials: MH
authors.lastname: Griep
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Cole
authors.forename: Shashi P
authors.initials: SP
authors.lastname: Karna
mesh_terms: D003850:Deoxyribonuclease I / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures; D013057:Spectrum Analysis
chemical_list: D006046:Gold; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I