title: Identification of a putative antifreeze protein gene that is highly expressed during preparation for winter in the sunn pest, Eurygaster maura.
abstract: A cDNA library generated from the fat body of field-collected, diapausing adults of the sunn pest, Eurygaster maura revealed the presence of a transcript that encodes a protein that shares the distinct physiochemical and structural features of an insect antifreeze protein. The transcript, which is most abundant in the midgut, accumulates in adults as they leave the fields in late summer and migrate to surrounding mountainous areas to overwinter. Transcript abundance again declines when adults return to the fields the following spring. This winter pattern of abundance suggests that this protein may be critical for winter survival in the cold regions where the bug enters its obligatory diapause.
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, University of Ankara, Ankara, Turkey. Electronic address: nurperguz@agri.ankara.edu.tr.
authors.forename: Nurper
authors.initials: N
authors.lastname: Guz
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, University of Ankara, Ankara, Turkey.
authors.forename: Umut
authors.initials: U
authors.lastname: Toprak
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, University of Ankara, Ankara, Turkey.
authors.forename: Asli
authors.initials: A
authors.lastname: Dageri
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, University of Ankara, Ankara, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Oktay Gurkan
authors.affiliation: Departments of Entomology and Evolution, Ecology and Organismal Biology, Ohio State University, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Denlinger
mesh_terms: D000596:Amino Acids; D000818:Animals; D021301:Antifreeze Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018076:DNA, Complementary; D064666:Diapause, Insect / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D020062:Heteroptera / Q000235:genetics* / Q000502:physiology*; D019476:Insect Proteins; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012621:Seasons; D014421:Turkey
chemical_list: D000596:Amino Acids; D021301:Antifreeze Proteins; D018076:DNA, Complementary; D019476:Insect Proteins
keywords: Antifreeze protein; Cold tolerance; Diapause; Sunn pest