title: Lrrc34, a novel nucleolar protein, interacts with npm1 and ncl and has an impact on pluripotent stem cells.
abstract: The gene Lrrc34 (leucine rich repeat containing 34) is highly expressed in pluripotent stem cells and its expression is strongly downregulated upon differentiation. These results let us to suggest a role for Lrrc34 in the regulation and maintenance of pluripotency. Expression analyses revealed that Lrrc34 is predominantly expressed in pluripotent stem cells and has an impact on the expression of known pluripotency genes, such as Oct4. Methylation studies of the Lrrc34 promoter showed a hypomethylation in undifferentiated stem cells and chromatin immunoprecipitation-quantitative polymerase chain reaction analyses of histone modifications revealed an enrichment of activating histone modifications on the Lrrc34 promoter region. Further, we could verify the nucleolus-the place of ribosome biogenesis-as the major subcellular localization of the LRRC34 protein. We have verified the interaction of LRRC34 with two major nucleolar proteins, Nucleophosmin and Nucleolin, by two independent methods, suggesting a role for Lrrc34 in ribosome biogenesis of pluripotent stem cells. In conclusion, LRRC34 is a novel nucleolar protein that is predominantly expressed in pluripotent stem cells. Its altered expression has an impact on pluripotency-regulating genes and it interacts with proteins known to be involved in ribosome biogenesis. Therefore we suggest a role for Lrrc34 in ribosome biogenesis of pluripotent stem cells.
authors.affiliation: 1 Institute of Human Genetics, University of Göttingen , Göttingen, Germany .
authors.forename: Sandra
authors.initials: S
authors.lastname: Lührig
authors.forename: Iliana
authors.initials: I
authors.lastname: Siamishi
authors.forename: Marieke
authors.initials: M
authors.lastname: Tesmer-Wolf
authors.forename: Ulrich
authors.initials: U
authors.lastname: Zechner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Engel
authors.forename: Jessica
authors.initials: J
authors.lastname: Nolte
mesh_terms: D000818:Animals; D019175:DNA Methylation / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000502:physiology; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D000097796:Nucleolin
chemical_list: D009687:Nuclear Proteins; D000090243:Nucleophosmin; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D000097796:Nucleolin; C000716967:NPM1 protein, human; C000715570:Npm1 protein, mouse; C000592432:leucine-rich repeat-containing protein 34, mouse