title: Prolactin and prolactin receptor expression in cervical intraepithelial neoplasia and cancer.
abstract: Prolactin receptor (PRLR) overexpression could play a role in tumorigenesis. The aim of this study was to determine prolactin (PRL) and PRLR expression in biopsies from patients with precursor lesions and uterine cervical cancer. PRLR expression was analyzed in 63 paraffin-embedded biopsies of uterine cervical tissue. In total, eleven low-grade squamous intraepithelial lesions (LSIL), 23 high-grade squamous intraepithelial lesions (HSIL), 21 uterine cervical cancers (UCC) and 8 normal epithelium (NE) were examined using immunoperoxidase staining and Western blot analysis. Additionally, PRL expression was identified in human cervical cancer serum and tissues. The PRLR expression was found to be significantly increased in cervical cancer in comparison with normal tissue and precursor lesions (P < 0.0003). The presence of the long isoform of the PRLR was observed only in cervical cancer tissues. Serum PRL levels were normal in all samples and local prolactin expression was similar in precursor lesions and cervical cancer by Western blot analysis. Our data suggest a possible role for PRLR in the progression of cervical cancer.
authors.affiliation: Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Rafael
authors.initials: R
authors.lastname: Ascencio-Cedillo
authors.forename: Edgar Ivan
authors.initials: EI
authors.lastname: López-Pulido
authors.forename: José Francisco
authors.initials: JF
authors.lastname: Muñoz-Valle
authors.forename: Nicolás
authors.initials: N
authors.lastname: Villegas-Sepúlveda
authors.forename: Susana
authors.initials: S
authors.lastname: Del Toro-Arreola
authors.forename: Ciro
authors.initials: C
authors.lastname: Estrada-Chávez
authors.forename: Adrian
authors.initials: A
authors.lastname: Daneri-Navarro
authors.forename: Ramón
authors.initials: R
authors.lastname: Franco-Topete
authors.forename: Delia
authors.initials: D
authors.lastname: Pérez-Montiel
authors.forename: Alejandro
authors.initials: A
authors.lastname: García-Carrancá
authors.forename: Ana Laura
authors.initials: AL
authors.lastname: Pereira-Suárez
mesh_terms: D001706:Biopsy; D045744:Cell Line, Tumor; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D060787:Neoplasm Grading; D011388:Prolactin / Q000235:genetics / Q000378:metabolism*; D011981:Receptors, Prolactin / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
chemical_list: D011981:Receptors, Prolactin; D011388:Prolactin