title: Water-soluble polysaccharides from Pleurotus ostreatus var. florida mycelial biomass.
abstract: Pleurotus ostreatus var. florida known as Hiratake has a high nutritional value, presents medicinal and nutraceutical properties and it is one of the consumed mushrooms in Brazil. Thus, the aim of this study was to characterize the chemical structure of polysaccharides found in mycelial biomass produced by submerged culture of P. ostreatus var. florida in order to compare with those found in P. ostreatus var. florida fruit bodies. Aqueous and alkali extracts obtained from mycelial biomass were purified, 13C NMR, GC-MS and chemical techniques were used to characterize three polysaccharide structures: a mannogalactan (MG-PfM) with α-D-Galp and 3-O-Me-α-D-Galp units, both (1→6)-linked, highly substituted at O-2 by D-Manp, a glycogen-like polymer (GLY-PfM) with α-D-Glp (1→4)-linked main chain, partially substituted at O-6 by α-D-Glcp side chains and a (1→3), (1→6) β-D-glucan (βGLC-PfM) with a main chain of β-D-Glcp (1→3)-linked units, partially substituted at O-6 by side chains of 6-O-substituted β-D-glucopyranosyl units, on an average of one to every two residues of the backbone. These results show the possibility to obtain similar and also different molecules from those found in the fruiting body of the same mushroom species, therefore the submerged culture of mushroom is a promising way to give raise molecules of interest.
authors.affiliation: Instituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo, 2936, CEP 69060-001 Manaus, AM, Brazil. Electronic address: leimybio@gmail.com.
authors.forename: Dirce L
authors.initials: DL
authors.lastname: Komura
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba CEP 81531-980, Brazil. Electronic address: andrearuthes@gmail.com.
authors.forename: Andrea C
authors.initials: AC
authors.lastname: Ruthes
authors.affiliation: Departamento de Química, Universidade Federal de Goiás, Campus Catalão, CEP 75704-020 Catalão, GO, Brazil. Electronic address: elainecarbonero@gmail.com.
authors.forename: Elaine R
authors.initials: ER
authors.lastname: Carbonero
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba CEP 81531-980, Brazil. Electronic address: cesarat@ufpr.br.
authors.forename: Philip A J
authors.initials: PA
authors.lastname: Gorin
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba CEP 81531-980, Brazil. Electronic address: iacomini@ufpr.br.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
mesh_terms: D018533:Biomass; D062610:Fungal Polysaccharides / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D006003:Glycogen / Q000737:chemistry; D008970:Molecular Weight; D025282:Mycelium / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D020076:Pleurotus / Q000737:chemistry*; D012995:Solubility
chemical_list: D062610:Fungal Polysaccharides; D005685:Galactans; D005936:Glucans; D006003:Glycogen
keywords: Glucans; Mannogalactan; Mycelia; Pleurotus spp.