title: Identification and characterization of androgenic gland specific insulin-like peptide-encoding transcripts in two spiny lobster species: Sagmariasus verreauxi and Jasus edwardsii.
abstract: In this study we describe, for the first time in spiny lobsters, the androgenic gland and its putative hormone. The androgenic gland in crustaceans is the key regulator of crustacean masculinity. The transcript encoding the insulin-like androgenic gland specific factor has recently been identified and characterized in a number of decapod crustacean species including commercially important crabs, crayfish, prawns and shrimps. This insulin-like factor has proven to be the androgenic gland masculinizing hormone, and is absent in females. While the androgenic gland and its putative hormone have been identified in all other commercially valuable groups, none had been identified in lobsters. We identified and characterized the androgenic glands of two spiny lobster species (Sagmariasus verreauxi and Jasus edwardsii) and conducted a transcriptomic analysis of the S. verreauxi androgenic gland. Bioinformatics analysis led to the discovery and characterization of the insulin-like androgenic gland specific factors in both species studied. Changes in androgenic gland cell size and quantity between sub-adult and sexually mature males were evident. The transcriptomic database established for the S. verreauxi androgenic gland might enable to elucidate the mechanisms through which the insulin-like factor is secreted, transported to the target cells and how it triggers the physiological effects of sexual differentiation towards maleness and maintenance of the male gonad.
authors.affiliation: GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, 4 Locked Bag, Maroochydore, Queensland 4558, Australia. Electronic address: tventura@usc.edu.au.
authors.forename: Tomer
authors.initials: T
authors.lastname: Ventura
authors.affiliation: Institute for Marine and Antarctic Studies, University of Tasmania, Private Bag 49, Hobart, Tasmania 7001, Australia.
authors.forename: Quinn
authors.initials: Q
authors.lastname: Fitzgibbon
authors.affiliation: Institute for Marine and Antarctic Studies, University of Tasmania, Private Bag 49, Hobart, Tasmania 7001, Australia.
authors.forename: Stephen
authors.initials: S
authors.lastname: Battaglene
authors.affiliation: Department of Life Sciences, The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva POB 653, Israel.
authors.forename: Amir
authors.initials: A
authors.lastname: Sagi
authors.affiliation: GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, 4 Locked Bag, Maroochydore, Queensland 4558, Australia.
authors.forename: Abigail
authors.initials: A
authors.lastname: Elizur
mesh_terms: D000595:Amino Acid Sequence; D000728:Androgens / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism*; D003001:Cloning, Molecular; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D042341:Gonadal Hormones / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D007328:Insulin / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D033521:Palinuridae / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D036361:Peptide Hormones / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation
chemical_list: D000728:Androgens; D015415:Biomarkers; D042341:Gonadal Hormones; D007328:Insulin; D036361:Peptide Hormones; D012333:RNA, Messenger
keywords: Androgenic gland; Insulin-like androgenic gland hormone; Jasus edwardsii; Sagmariasus verreauxi; Spiny lobster