title: Glycan based detection and drug susceptibility of influenza virus.
abstract: We have developed a panel of synthetic glycans as receptor mimics for the specific capture of influenza viruses. The glycans were printed onto commercial glass slides using a free amine at the end of a spacer to generate a small focused microarray. The microarray was evaluated for its ability to capture three different strains of influenza A virus, two H1N1, A/Brisbane/59/2007 and A/Solomon Islands/3/2006 and one H3N2, A/Aichi/2/1968. We observed an excellent detection ability with some compounds exhibiting clinically relevant (10(1) plaque forming units) limit of detection. We also tested the drug susceptibility of current antivirals, Zanamivir and Ostelamivir using this microarray and could determine antiviral resistance for these strains.
authors.affiliation: 788 Petit Science Center, Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University , Atlanta, Georgia 30302, United States.
authors.forename: Hieu
authors.initials: H
authors.lastname: Dinh
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Zhang
authors.forename: Joyce
authors.initials: J
authors.lastname: Sweeney
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: He
authors.forename: Abasaheb
authors.initials: A
authors.lastname: Dhawane
authors.forename: Suri S
authors.initials: SS
authors.lastname: Iyer
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D015374:Biosensing Techniques / Q000295:instrumentation*; D024882:Drug Resistance, Viral; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects / Q000302:isolation & purification; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects / Q000302:isolation & purification; D009980:Influenza A virus / Q000187:drug effects / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000188:drug therapy / Q000821:virology; D057230:Limit of Detection; D046228:Microarray Analysis / Q000295:instrumentation*; D008826:Microbial Sensitivity Tests; D053139:Oseltamivir / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry*; D053243:Zanamivir / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D011134:Polysaccharides; D053139:Oseltamivir; D053243:Zanamivir