title: ISO-based assessment of accuracy and precision of glucose meters in dogs.
abstract: BACKGROUND
  Portable blood glucose meters (PBGMs) allow easy glucose measurements. As animal-specific PBGMs are not available everywhere, those for humans are widely used.
  
  
  OBJECTIVE
  To assess the accuracy and precision of 9 PBGMs in canine whole blood (WB) and plasma, based on the ISO 15197:2013.
  
  
  METHODS
  Fifty-nine client-owned dogs attending the Veterinary Teaching Hospital.
  
  
  METHODS
  Analytical evaluation of 100 blood samples was performed for accuracy and 23 for precision (glucose 29-579 mg/dL) following ISO recommendations. A PBGM was considered accurate if 95% of the measurements were within ±15 mg/dL from the reference when glucose was <100 mg/dL and within ±15% when it was ≥100 mg/dL, and if 99% of them were within zones A and B in error grid analysis (EG). A hexokinase-based analyzer was used as reference. Ninety samples were assessed for hematocrit interferences.
  
  
  RESULTS
  Accuracy requirements were not fulfilled by any PBGM in WB (74% of measurements within the limits for the most accurate) and by 1 only in plasma. However, the EG analysis in WB was passed by 6 PBGM and by all in plasma. The most accurate were also the most precise, with coefficients of variation <5% in WB and <3% in plasma. Hematocrit correlated with bias against the reference method in 4 PBGM (r = -0.243 - [-0.371]; P < .021).
  
  
  CONCLUSIONS
  This disparity among PBGM suggests that meters approved for humans need to be evaluated before use in other species.
authors.affiliation: Servicio de Endocrinología y Nutrición, Complejo Hospitalario Universitario Insular Materno Infantil de Gran Canaria, Las Palmas de Gran Canaria, Spain; Instituto Universitario de Investigaciones Biomédicas y Sanitarias, Universidad de Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Spain.
authors.forename: Y
authors.initials: Y
authors.lastname: Brito-Casillas
authors.forename: P
authors.initials: P
authors.lastname: Figueirinhas
authors.forename: J C
authors.initials: JC
authors.lastname: Wiebe
authors.forename: L
authors.initials: L
authors.lastname: López-Ríos
authors.forename: D
authors.initials: D
authors.lastname: Pérez-Barreto
authors.forename: C
authors.initials: C
authors.lastname: Melián
authors.forename: A M
authors.initials: AM
authors.lastname: Wägner
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D004283:Dog Diseases / Q000097:blood; D004285:Dogs / Q000097:blood*; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000592:standards / Q000662:veterinary*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D001786:Blood Glucose
keywords: Diabetes; Dog; ISO 15197:2003; ISO 15197:2013