title: Advanced mass spectrometry technologies for the study of microbial pathogenesis.
abstract: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has been successfully applied to the field of microbial pathogenesis with promising results, principally in diagnostic microbiology to rapidly identify bacteria based on the molecular profiles of small cell populations. Direct profiling of molecules from serum and tissue samples by MALDI MS provides a means to study the pathogen-host interaction and to discover potential markers of infection. Systematic molecular profiling across tissue sections represents a new imaging modality, enabling regiospecific molecular measurements to be made in situ, in both two-dimensional and three-dimensional analyses. Herein, we briefly summarize work that employs MALDI MS to study the pathogenesis of microbial infection.
authors.affiliation: Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, United States.
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Moore
authors.affiliation: Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, United States. Electronic address: R.Caprioli@Vanderbilt.edu.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Caprioli
authors.affiliation: Department of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, TN, United States. Electronic address: Eric.Skaar@Vanderbilt.edu.
authors.forename: Eric P
authors.initials: EP
authors.lastname: Skaar
mesh_terms: D000818:Animals; D054884:Host-Pathogen Interactions; D006801:Humans; D013058:Mass Spectrometry; D008828:Microbiological Techniques / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization