title: Metabolites analysis, metabolic enzyme activities and bioaccumulation in the clam Ruditapes philippinarum exposed to benzo[a]pyrene.
abstract: A study was performed on clams (Ruditapes philippinarum) exposed to 0.03, 0.3 and 3μg/L benzo[a]pyrene (B[a]P) for 21 days. B[a]P metabolite contents, activities of aryl hydrocarbon hydroxylase (AHH), 7-ethoxyresorufin O-deethylase (EROD), epoxide hydrolase (EH), dihydrodiol dehydrogenase (DD), glutathione-S-transferase (GST), sulfotransferase (SULT) and uridinediphosphate glucuronyltransferase (UGT) and B[a]P bioaccumulation were assayed in gills and digestive glands. Results showed that the order of B[a]P phase I metabolite contents was 9-hydroxy-B[a]P>B[a]P-1,6-dione>B[a]P-7,8-dihydrodiol, and the concentration of B[a]P-7,8-dihydrodiol sulfate conjugates was higher than that of B[a]P-7,8-dihydrodiol glucuronide conjugates. B[a]P accumulation and the activities of AHH, EROD, EH, DD, SULT and UGT increased first and then reached equilibrium. GST activity was induced first and then depressed. The concentration of B[a]P was far higher than that of its metabolites. Besides, there were no significant differences between enzyme activities in gills and those in digestive glands. These results provided information on B[a]P metabolic mechanism in bivalve and scientific data for pollution monitoring and food security.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Cai
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Miao
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D049872:Bivalvia / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004784:Environmental Monitoring; D005260:Female; D005880:Gills / Q000737:chemistry / Q000201:enzymology; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D012758:Shellfish / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase
keywords: Benzo[a]pyrene; Bioaccumulation; Detoxification; Metabolite; Ruditapes philippinarum