title: The application of chromosome abnormality chip detection in male infertility.
abstract: To discuss the application of microarray technology in the diagnosis of male infertility.
  
  
  
  Sixteen loci, including a sex-determining region on the Y chromosome, were investigated by polymerase chain reaction (PCR) in infertile male patients. Chromosome abnormality chip with 180 000 probes was used to detect small deletion, small amplification and loss of heterozygosity.
  
  
  
  By PCR, nine of 103 infertile patients were found to have sequence-tagged site microdeletions. Microdeletions were not observed in control samples. The deletions detected by PCR were present in six azoospermic men (6/44, 13.6%) and in three oligoasthenoteratozoospermic (OATS) men (3/59, 5%).The overall frequency of microdeletions in infertile men was 8.7% (9/103). Chromosome abnormality chip detection 500+ detected more amplification or deletion in 51 infertile patients and the overall frequency of microdeletions in infertile men was 49.5% (51/103).
  
  
  
  Chromosome abnormality chip detection system provides a sensitive, economic and high-throughput method for detecting the deletion or amplification of genomic DNA sequences of infertile patients. Not only can it identify Yq deletions, but it can also find other chromosome abnormalities and facilitate the understanding of male infertility.
authors.affiliation: Yantai Yuhuangding Hospital Biochip Laboratory, Shandong Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Yantai Yuhuangding Hospital Center Laboratory, Shandong Province, China.
authors.forename: Wt
authors.initials: W
authors.lastname: Wang
authors.affiliation: Yantai Yuhuangding Hospital Examination Center, Shandong Province, China.
authors.forename: Rm
authors.initials: R
authors.lastname: Lui
authors.affiliation: Yantai Yuhuangding Hospital Biochip Laboratory, Shandong Province, China.
authors.forename: Sx
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Yantai Yuhuangding Hospital Examination Center, Shandong Province, China.
authors.forename: Xb
authors.initials: X
authors.lastname: Wang
authors.affiliation: Yantai Yuhuangding Hospital Biochip Laboratory, Shandong Province, China.
authors.forename: L
authors.initials: L
authors.lastname: Gong
authors.affiliation: Yantai Yuhuangding Hospital Biochip Laboratory, Shandong Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.affiliation: Yantai Yuhuangding Hospital Biochip Laboratory, Shandong Province, China.
authors.forename: Lj
authors.initials: Lj
authors.lastname: Duan
authors.affiliation: Yantai Yuhuangding Hospital Biochip Laboratory, Shandong Province, China.liulocus@126.com.
authors.forename: C M
authors.initials: CM
authors.lastname: Sun