title: Structure of Escherichia coli Grx2 in complex with glutathione: a dual-function hybrid of glutaredoxin and glutathione S-transferase.
abstract: The structure of glutaredoxin 2 (Grx2) from Escherichia coli co-crystallized with glutathione (GSH) was solved at 1.60 Å resolution. The structure of a mutant with the active-site residues Cys9 and Cys12 changed to serine crystallized in the absence of glutathione was solved to 2.4 Å resolution. Grx2 has an N-terminal domain characteristic of glutaredoxins, and the overall structure is congruent with the structure of glutathione S-transferases (GSTs). Purified Grx2 exhibited GST activity. Grx2, which is the physiological electron donor for arsenate reduction by E. coli ArsC, was docked with ArsC. The docked structure could be fitted with GSH bridging the active sites of the two proteins. It is proposed that Grx2 is a novel Grx/GST hybrid that functions in two steps of the ArsC catalytic cycle: as a GST it catalyzes glutathionylation of the ArsC-As(V) intermediate and as a glutaredoxin it catalyzes deglutathionylation of the ArsC-As(III)-SG intermediate.
authors.affiliation: Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Cellular Biology and Pharmacology, Florida International University, Herbert Wertheim College of Medicine, Miami, FL 33199, USA.
authors.forename: S Venkadesh
authors.initials: SV
authors.lastname: Nadar
authors.affiliation: Department of Cellular Biology and Pharmacology, Florida International University, Herbert Wertheim College of Medicine, Miami, FL 33199, USA.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cellular Biology and Pharmacology, Florida International University, Herbert Wertheim College of Medicine, Miami, FL 33199, USA.
authors.forename: Barry P
authors.initials: BP
authors.lastname: Rosen
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry*; D054477:Glutaredoxins / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D054477:Glutaredoxins; D005978:Glutathione
keywords: glutaredoxin 2; glutaredoxin/glutathione S-transferase hybrid; glutathione