title: Comparing GC-MS, HPLC and ¹H NMR analysis of beef longissimus dorsi tissue extracts to determine the effect of suspension technique and ageing.
abstract: Beef longissimus dorsi muscle samples matured over a 21 day period were analysed using three different analytical techniques; (1)H NMR, GC-MS and HPLC. The data from the three experimental techniques were correlated with each other to determine if the results were statistically similar to each other. From our analysis we determined that the metabolites measured using (1)H NMR were statistically similar to the compounds quantified using the chromatography techniques (p<0.001). In addition, using PCA, we were able to show that different metabolites, measured using the various analytical techniques produced very similar scores and loadings plots for all the analysis and extraction techniques undertaken across the 21 day time domain. Using a combination of these three different techniques provides a unique and holistic insight into the biochemistry behind the conversion of muscle to meat which would not be possible using any single technique alone.
authors.affiliation: Institute of Agri-Food and Land Use, Queen's University Belfast, Stranmillis Road, Belfast, Northern Ireland BT9 5AG, United Kingdom.
authors.forename: Stewart Francis
authors.initials: SF
authors.lastname: Graham
authors.affiliation: Agri-Food and Biosciences Institute, Newforge Lane, Belfast, Northern Ireland, United Kingdom.
authors.forename: David
authors.initials: D
authors.lastname: Farrell
authors.affiliation: Agri-Food and Biosciences Institute, Newforge Lane, Belfast, Northern Ireland, United Kingdom.
authors.forename: Terry
authors.initials: T
authors.lastname: Kennedy
authors.affiliation: Agri-Food and Biosciences Institute, Newforge Lane, Belfast, Northern Ireland, United Kingdom.
authors.forename: Alan
authors.initials: A
authors.lastname: Gordon
authors.affiliation: Institute of Agri-Food and Land Use, Queen's University Belfast, Stranmillis Road, Belfast, Northern Ireland BT9 5AG, United Kingdom; Agri-Food and Biosciences Institute, Newforge Lane, Belfast, Northern Ireland, United Kingdom.
authors.forename: Linda
authors.initials: L
authors.lastname: Farmer
authors.affiliation: Institute of Agri-Food and Land Use, Queen's University Belfast, Stranmillis Road, Belfast, Northern Ireland BT9 5AG, United Kingdom.
authors.forename: Christopher
authors.initials: C
authors.lastname: Elliott
authors.affiliation: Institute of Agri-Food and Land Use, Queen's University Belfast, Stranmillis Road, Belfast, Northern Ireland BT9 5AG, United Kingdom; Agri-Food and Biosciences Institute, Newforge Lane, Belfast, Northern Ireland, United Kingdom.
authors.forename: Bruce
authors.initials: B
authors.lastname: Moss
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D025341:Principal Component Analysis; D066244:Proton Magnetic Resonance Spectroscopy; D014020:Tissue Extracts / Q000032:analysis*
chemical_list: D014020:Tissue Extracts