title: [Establishment and identification of a H-2 completely mismatched microtransplantation model of leukemia mouse].
abstract: This study was purposed to establish and identify a H-2 completely mismatched microtransplantation model of leukemia mouse. The recipients were female BALB/c mice, while donors were male C57BL/6J mice. Recipients were inoculated intravenously with 1×10(6) of WEHI-3 cells, a cell line of myelomonocytic leukemia. Donors received 100 µg/kg G-CSF mobilization through hypodermic injection, every 12 hours, and it last 5 days. Chemotherapy regimens was MA (mitoxantrone+cytarabine), and it last 4 days. Recipients were given chemotherapy conditioning without GVHD prophylaxis after inoculation of leukemic cells for 2 days, and within 8 hours after last chemotherapy received donor mobilized spleen mononuclear cells (sMNC). The number of sMNC was (3, 6, 12) ×10(7), respectively. The early death rate, recovery level of WBC in peripheral blood and leukemia load were compared between chemotherapy and microtransplantation groups. The donor chimerism was detected by RT-PCR. From the clinical manifestation and pathological features, the GVHD in recipients was evaluated. The results showed that the early mortality in chemotherapy group was 25%, meanwhile those in the (3, 6, 12)×10(7) groups were 16.67%, 8.33%, 8.33%, respectively. The(3, 6)×10(7) groups has a stronger hematopoietic recovery capability than that in chemotherapy and 12×10(7) groups (P < 0.05) . There were more leukemic cells in chemotherapy mice than that in microtransplantation mice (P < 0.01) , and (12, 6)×10(7) groups had lower leukemia load than that in 3×10(7) group (P < 0.05) . No signs of GVHD were observed in microtransplantation mice. The donor microchimerism could be discovered at eraly 2 weeks after donor cell transfusion. It is concluded that a H-2 completely mismatched microtransplantation model of leukemia mouse has been successfully established, and it will provide a experimental base for studying microtransplantation in clinic.
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Wu
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Tie-Qiang
authors.initials: TQ
authors.lastname: Liu
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Xue-Dong
authors.initials: XD
authors.lastname: Sun
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Huang
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Man
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Ya-Jing
authors.initials: YJ
authors.lastname: Huang
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Qi-Yun
authors.initials: QY
authors.lastname: Sun
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Zuo
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Qiao
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Chang-Lin
authors.initials: CL
authors.lastname: Yu
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Kai-Xun
authors.initials: KX
authors.lastname: Hu
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Hui-Sheng
authors.initials: HS
authors.lastname: Ai
authors.affiliation: Department of Hematology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China. E-mail: guom196801@aliyun.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D007938:Leukemia / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018183:Transplantation Chimera; D014184:Transplantation, Homologous