title: Preparation and biological activity of quaternized carboxymethyl chitosan conjugated with collagen peptide.
abstract: Tissue repair is a spontaneous process which initiated on wounding. If this complex mechanism is disturbed or impaired, the use of biomaterials might increase the chance of successful healing. In this view, a water-soluble chitosan derivative, quaternized carboxymethyl chitosan (QCMC) was prepared and collagen peptides (COPs) were grafted to the backbone by carbodiimide method. The reaction conditions affecting the degree of substitution (DS) were studied including the mass ratio of collagen peptide to QCMC, reaction temperature and reaction time. The hydrogen peroxide-scavenging activity could be different by changing the DS, concentration and molecular weight. MTT assay was used to investigate the cell viability of the derivative. The results indicated that the introduction of collagen peptide into the QCMC improved its hydrogen peroxide-scavenging activity and cell viability with the DS and concentration increased. Therefore, QCMC conjugated with collagen peptides may prove beneficial to the process of the wound-healing.
authors.affiliation: Hubei Collaboration Innovation Center of Non-power Nuclear Technology, Hubei University of Science and Technology, Xianning 437100, China; School of Nuclear Technology & Chemistry and Biology, Hubei University of Science and Technology, Xianning 437100, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhu
authors.affiliation: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Jiayan
authors.initials: J
authors.lastname: Yi
authors.affiliation: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tong
authors.affiliation: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wu
authors.affiliation: Hubei Collaboration Innovation Center of Non-power Nuclear Technology, Hubei University of Science and Technology, Xianning 437100, China; College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China. Electronic address: lihongfan2000@hotmail.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry*; D005399:Fishes; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D051379:Mice; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D016166:Free Radical Scavengers; D010446:Peptide Fragments; C514968:carboxymethyl-chitosan; D003094:Collagen; D048271:Chitosan; D006861:Hydrogen Peroxide
keywords: Antioxidant activity; Cell viability; Collagen peptide; Quaternized carboxymethyl chitosan