title: MiR-15b targets cyclin D1 to regulate proliferation and apoptosis in glioma cells.
abstract: OBJECTIVE
  To investigate the role and mechanism of miR-15b in the proliferation and apoptosis of glioma.
  
  
  METHODS
  The miR-15b mimics were transfected into human glioma cells to upregulate the miR-15b expression. Cyclin D1 was determined by both western blotting analysis and luciferase reporter assay. Methylthiazol tetrazolium (MTT) and flow cytometry were employed to detect the cell proliferation, cell cycle, and apoptosis.
  
  
  RESULTS
  Overexpression of miR-15b inhibits proliferation by arrested cell cycle progression and induces apoptosis, possibly by directly targeting Cyclin D1. Both luciferase assay and bioinformatics search revealed a putative target site of miR-15b binding to the 3'-UTR of Cyclin D1. Moreover, expression of miR-15b in glioma tissues was found to be inversely correlated with Cyclin D1 expression. Enforced Cyclin D1 could abrogate the miR-15b-mediated cell cycle arrest and apoptosis.
  
  
  CONCLUSIONS
  Our findings identified that miR-15b may function as a glioma suppressor by targeting the Cyclin D1, which may provide a novel therapeutic strategy for treatment of glioma.
authors.affiliation: Department of Neurosurgery, Fourth Affiliated Hospital of Nantong University, First Hospital of Yancheng, Yancheng 224001, China.
authors.forename: Guan
authors.initials: G
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215300, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Medical Oncology, The Eighty-First Hospital of People's Liberation Army, Nanjing 210002, China.
authors.forename: Shushan
authors.initials: S
authors.lastname: Yan
authors.affiliation: Department of Neurosurgery, Fourth Affiliated Hospital of Nantong University, First Hospital of Yancheng, Yancheng 224001, China.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Wan
authors.affiliation: Department of Neurosurgery, Fourth Affiliated Hospital of Nantong University, First Hospital of Yancheng, Yancheng 224001, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, Fourth Affiliated Hospital of Nantong University, First Hospital of Yancheng, Yancheng 224001, China.
authors.forename: Linshan
authors.initials: L
authors.lastname: Fu
authors.affiliation: Department of Neurosurgery, Jiangning Hospital Affiliated with Nanjing Medical University, Nanjing 211100, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Fourth Affiliated Hospital of Nantong University, First Hospital of Yancheng, Yancheng 224001, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D019938:Cyclin D1 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*
chemical_list: C505361:MIRN15 microRNA, human; D035683:MicroRNAs; D019938:Cyclin D1