title: Trehalose-based eye drops preserve viability and functionality of cultured human corneal epithelial cells during desiccation.
abstract: This paper presents the evaluation of cytoprotective ability of trehalose-based eye drops in comparison with commercially available preparations during the experimental desiccation of cultured human corneal epithelial cells. Cultured human corneal epithelial cells (hCEC) underwent incubation with 7 different, commercially available medicaments used commonly in dry eye syndrome treatment, followed by desiccation trial performed on air under the flow hood for 5, 15, 30, and 45 minutes. Cell viability was quantified by live/dead fluorescent assay, while the presence of apoptotic cells was estimated by immunofluorescent staining for active caspase 3 protein. The preservation of membrane functions was evaluated using neutral red staining, while the preservation of proper morphology and phenotype was determined by fluorescent staining for actin filaments, nuclei, and p63 protein. The trehalose-based eye drops showed the highest efficiency in prevention of cell death from desiccation; moreover, this preparation preserved the normal cellular morphology, functions of cell membrane, and proliferative activity more effectively than other tested medicaments.
authors.affiliation: Department of Ophthalmology, Medical University Wroclaw, Borowska 213, 50-556 Wroclaw, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Hill-Bator
authors.affiliation: Department of Ophthalmology, Medical University Wroclaw, Borowska 213, 50-556 Wroclaw, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Misiuk-Hojło
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences in Wroclaw, Kozuchowska 5b, 51-631 Wroclaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
authors.affiliation: Electron Microscopy Laboratory, University of Environmental and Life Sciences in Wroclaw, Kozuchowska 5b, 51-631 Wroclaw, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Grzesiak
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003890:Desiccation; D015352:Dry Eye Syndromes / Q000188:drug therapy* / Q000473:pathology; D019573:Epithelium, Corneal / Q000187:drug effects*; D006801:Humans; D009883:Ophthalmic Solutions / Q000008:administration & dosage* / Q000737:chemistry; D014199:Trehalose / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D009883:Ophthalmic Solutions; D014199:Trehalose