title: Crystallization and preliminary crystallographic analysis of histamine dehydrogenase from Natrinema gari BCC 24369.
abstract: Histamine dehydrogenase (HADH) catalyzes the oxidative deamination of histamine, resulting in the production of imidazole acetaldehyde and an ammonium ion. The enzyme isolated from the newly identified halophilic archaeon Natrinema gari BCC 24369 is significantly different from the previously described protein from Nocardioides simplex. This newly identified HADH comprises three subunits with molecular weights of 49.0, 24.7 and 23.9 kDa, respectively, and is optimally active under high-salt conditions (3.5-5 M NaCl). As a step in the exploration of the unique properties of the protein, the HADH heterotrimer was purified and crystallized. Crystals were obtained using the sitting-drop vapor-diffusion method from a solution composed of 0.2 M calcium chloride dihydrate, 0.1 M HEPES pH 7.5, 28% PEG 400. Diffraction data were collected at -173°C to a resolution limit of 2.4 Å on the Southeast Regional Collaborative Access Team (SER-CAT) beamline 22-ID at the Advanced Photon Source, Argonne National Laboratory. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a=211.9, b=58.6, c=135.4 Å, β=103.0°. The estimated Matthews coefficient is 3.21 Å3 Da(-1), corresponding to 61.7% solvent content.
authors.affiliation: Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA.
authors.forename: Dongwen
authors.initials: D
authors.lastname: Zhou
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand.
authors.forename: Wonnop
authors.initials: W
authors.lastname: Visessanguan
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand.
authors.forename: Siriporn
authors.initials: S
authors.lastname: Chaikaew
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Soottawat
authors.initials: S
authors.lastname: Benjakul
authors.affiliation: Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Oda
authors.affiliation: Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wlodawer
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D006216:Halobacteriaceae / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D008970:Molecular Weight; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D055503:Protein Multimerization; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D021122:Protein Subunits; D000587:Oxidoreductases Acting on CH-NH Group Donors; C506665:histamine dehydrogenase
keywords: Natrinema gari BCC 24369; histamine dehydrogenase