title: Hairpin DNA-assisted silicon/silver-based surface-enhanced Raman scattering sensing platform for ultrahighly sensitive and specific discrimination of deafness mutations in a real system.
abstract: Surface-enhanced Raman scattering (SERS) is well-recognized as a powerful analytical tool for ultrahighly sensitive detection of analytes. In this article, we present a kind of silicon-based SERS sensing platform made of a hairpin DNA-modified silver nanoparticles decorated silicon wafer (AgNPs@Si). In particular, the AgNPs@Si with a high enhancement factor (EF) value of ~4.5 × 10(7) is first achieved under optimum reaction conditions (i.e., pH = 12, reaction time = 20 min) based on systematic investigation. Such resultant AgNPs@Si is then employed for construction of a silicon-based SERS sensing platform through surface modification of hairpin DNA, which is superbly suitable for highly reproducible, multiplexed, and ultrasensitive DNA detection. A detection limit of 1 fM is readily achieved in a very reproducible manner along with high specificity. Most significantly, for the first time, we demonstrate that the silicon-based SERS platform is highly efficacious for discriminating deafness-causing mutations in a real system at the femtomolar level (500 fM), which is about 3-4 orders of magnitude lower than that (~5 nM) ever reported by conventional detection methods. Our results raise the exciting potential of practical SERS applications in biology and biomedicine.
authors.affiliation: Institute of Functional Nano and Soft Materials and Collaborative Innovation Center of Suzhou Nano Science and Technology, Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, and ‡Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, First Affiliated Hospital, and Collaborative Innovation Center of Hematology, Soochow University , Suzhou 215123, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xiangxu
authors.initials: X
authors.lastname: Jiang
authors.forename: Xing
authors.initials: X
authors.lastname: Wang
authors.forename: Xinpan
authors.initials: X
authors.lastname: Wei
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Su
authors.forename: Sudan
authors.initials: S
authors.lastname: He
authors.forename: Yao
authors.initials: Y
authors.lastname: He
mesh_terms: D004247:DNA / Q000737:chemistry*; D003638:Deafness / Q000235:genetics*; D006801:Humans; D057230:Limit of Detection; D009154:Mutation; D012825:Silicon / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D012834:Silver; D004247:DNA; D012825:Silicon