title: Managing oxidative stresses in Shewanella oneidensis:  intertwined roles of the OxyR and OhrR regulons.
abstract: Shewanella oneidensis, renowned for its remarkable respiratory abilities, inhabit redox-stratified environments prone to reactive oxygen species (ROS)formation. Two major oxidative stress regulators,analogues of OxyR and OhrR, specifically respond to H(2)O(2) and organic peroxides (OP), respectively, are encoded in the genome based on sequence comparison to well-studied models. Presumably, these analogues provide protection from ROS. An understanding of S. oneidensis OxyR has been established recently, which functions as both repressor and activator to mediate H(2)O(2)-induced oxidative stress. Here,we report the first study of elucidating molecular mechanisms underlying the S. oneidensis response to OP-induced oxidative stress. We show tha tS. oneidensis has OhrR, an OP stress regulator with two novel features. The sensing and responding residues of OhrR are not equally important for regulation and the regulator directly controls transcription of the SO1563 gene, in addition to the ohr gene which encodes the major OP scavenging protein. Importantly,we present evidence suggesting that the OxyR and OhrR regulons of S. oneidensis appear to be functionally intertwined as both OxyR and OhrR systems can sense and response to H(2)O(2) and OP agents.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Qixia
authors.initials: Q
authors.lastname: Luo
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Jiang
authors.forename: Genfu
authors.initials: G
authors.lastname: Wu
authors.forename: Haichun
authors.initials: H
authors.lastname: Gao
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D016384:Consensus Sequence; D015964:Gene Expression Regulation, Bacterial; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics*; D018085:Regulon; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D020592:Shewanella / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; D006861:Hydrogen Peroxide