title: Effect of detorsion alone and in combination with enoxaparin therapy on ovarian reserve and serum antimüllerian hormone levels in a rat ovarian torsion model.
abstract: OBJECTIVE
  To investigate the effect of enoxaparin on ovarian reserve and serum antimüllerian hormone (AMH) levels in a rat ovarian torsion model.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Experimental surgery laboratory in a training and research hospital.
  
  
  METHODS
  Fourteen female Wistar Hannover rats.
  
  
  METHODS
  1) Control group received no special treatment other than abdominal exposure; 2) detorsion-only group received bilateral adnexal torsion (3-hour ischemia), and then after 3-hour torsion period, detorsion (reperfusion) was performed; and 3) detorsion-enoxaparin group received 0.5 mg/kg enoxaparin subcutaneously 2 hours before the same surgery as the detorsion-only group and a second 0.5 mg/kg dose of enoxaparin 24 hours after the first surgeries. Apart from the surgeries, preoperative and postoperative 1-mL blood samples were drawn from the right jugular vein of each rat.
  
  
  METHODS
  Preoperative and postoperative serum AMH levels, histopathologic damage scores, and follicle counts in the ovarian tissue of the rats.
  
  
  RESULTS
  Vascular congestion and hemorrhage scores were higher in the detorsion-enoxaparin group than in the detorsion-only and control groups. The number of small antral follicles was smaller in the detorsion-only group than in the control group. The difference in the pre- and postoperative AMH levels was higher in the detorsion-only group than in the control and detorsion-enoxaparin groups.
  
  
  CONCLUSIONS
  The combination of enoxaparin therapy with conventional ovarian detorsion is more effective in protecting the ovarian reserve than detorsion alone.
authors.affiliation: Clinic of Obstetrics and Gynecology, Van Özalp State Hospital, Van, Turkey. Electronic address: drcihankaya@gmail.com.
authors.forename: Cihan
authors.initials: C
authors.lastname: Kaya
authors.affiliation: Department of Pathology, Bakırköy Dr. Sadi Konuk Training and Research Hospital, Istanbul, Turkey.
authors.forename: Hürriyet
authors.initials: H
authors.lastname: Turgut
authors.affiliation: Department of Obstetrics and Gynecology, Bakırköy Dr. Sadi Konuk Training and Research Hospital, Istanbul, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Cengiz
authors.affiliation: Division of Midwifery, Faculty of Health Sciences, İstanbul University, Istanbul, Turkey.
authors.forename: Ayşenur
authors.initials: A
authors.lastname: Turan
authors.affiliation: Department of Obstetrics and Gynecology, Bakırköy Dr. Sadi Konuk Training and Research Hospital, Istanbul, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Ekin
authors.affiliation: Department of Obstetrics and Gynecology, Bakırköy Dr. Sadi Konuk Training and Research Hospital, Istanbul, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Yaşar
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood*; D002452:Cell Count; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D017984:Enoxaparin / Q000627:therapeutic use*; D005184:Fallopian Tube Diseases / Q000097:blood / Q000188:drug therapy* / Q000601:surgery*; D005260:Female; D013509:Gynecologic Surgical Procedures / Q000379:methods*; D010053:Ovary / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control; D014102:Torsion Abnormality / Q000097:blood / Q000188:drug therapy* / Q000601:surgery*
chemical_list: D017984:Enoxaparin; D054304:Anti-Mullerian Hormone
keywords: Antimüllerian hormone; enoxaparin; ischemia; ovarian follicle; ovary