title: Silencing [Formula: see text] Rescues Tau Pathologies and Memory Deficits through Rescuing PP2A and Inhibiting GSK-3β Signaling in Human Tau Transgenic Mice.
abstract: Increase of inhibitor-2 of protein phosphatase-2A [Formula: see text] is associated with protein phosphatase-2A (PP2A) inhibition and tau hyperphosphorylation in Alzheimer's disease (AD). Down-regulating [Formula: see text] attenuated amyloidogenesis and improved the cognitive functions in transgenic mice expressing amyloid precursor protein (tg2576). Here, we found that silencing [Formula: see text] by hippocampal infusion of [Formula: see text] down-regulated [Formula: see text] (~45%) with reduction of tau phosphorylation/accumulation, improvement of memory deficits, and dendritic plasticity in 12-month-old human tau transgenic mice. Silencing [Formula: see text] not only restored PP2A activity but also inhibited glycogen synthase kinase-3β (GSK-3β) with a significant activation of protein kinase A (PKA) and Akt. In HEK293/tau and N2a/tau cells, silencing [Formula: see text] by [Formula: see text] also significantly reduced tau hyperphosphorylation with restoration of PP2A activity and inhibition of GSK-3β, demonstrated by the decreased GSK-3β total protein and mRNA levels, and the increased inhibitory phosphorylation of GSK-3β at serine-9. Furthermore, activation of PKA but not Akt mediated the inhibition of GSK-3β by [Formula: see text] silencing. We conclude that targeting [Formula: see text] can improve tau pathologies and memory deficits in human tau transgenic mice, and activation of PKA contributes to GSK-3β inhibition induced by silencing [Formula: see text]in vitro, suggesting that [Formula: see text] is a promising multiple target of AD.
authors.affiliation: Key Laboratory of Neurological Disease of Chinese Ministry of Education, Huazhong University of Science and Technology, Wuhan, China.|Department of Endocrinology, Liyuan Hospital, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Rong-Hong
authors.initials: RH
authors.lastname: Ma
authors.affiliation: Key Laboratory of Neurological Disease of Chinese Ministry of Education, Huazhong University of Science and Technology, Wuhan, China.|Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Xia-Chun
authors.initials: XC
authors.lastname: Li
authors.affiliation: Key Laboratory of Neurological Disease of Chinese Ministry of Education, Huazhong University of Science and Technology, Wuhan, China.|Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Neurological Disease of Chinese Ministry of Education, Huazhong University of Science and Technology, Wuhan, China.|Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Hai-Rong
authors.initials: HR
authors.lastname: Shi
authors.affiliation: Key Laboratory of Neurological Disease of Chinese Ministry of Education, Huazhong University of Science and Technology, Wuhan, China.|Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.|Department of Pathophysiology, Jinan University, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Key Laboratory of Neurological Disease of Chinese Ministry of Education, Huazhong University of Science and Technology, Wuhan, China.|Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Dan-Ju
authors.initials: DJ
authors.lastname: Luo
authors.affiliation: Key Laboratory of Neurological Disease of Chinese Ministry of Education, Huazhong University of Science and Technology, Wuhan, China.|Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Neurological Disease of Chinese Ministry of Education, Huazhong University of Science and Technology, Wuhan, China.|Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Jian-Zhi
authors.initials: JZ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Neurological Disease of Chinese Ministry of Education, Huazhong University of Science and Technology, Wuhan, China.|Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Gong-Ping
authors.initials: GP
authors.lastname: Liu
keywords: Alzheimer disease; glycogen synthase kinase-3β; inhibitor-2 of protein phosphatase-2A; memory; tau hyperphosphorylation