title: An adaptable mesocosm platform for performing integrated assessments of nanomaterial risk in complex environmental systems.
abstract: Physical-chemists, (micro)biologists, and ecologists need to conduct meaningful experiments to study the environmental risk of engineered nanomaterials with access to relevant mechanistic data across several spatial and temporal scales. Indoor aquatic mesocosms (60L) that can be tailored to virtually mimic any ecosystem appear as a particularly well-suited device. Here, this concept is illustrated by a pilot study aimed at assessing the distribution of a CeO₂-based nanomaterial within our system at low concentration (1.5 mg/L). Physico-chemical as well as microbiological parameters took two weeks to equilibrate. These parameters were found to be reproducible across the 9-mesocosm setup over a 45-day period of time. Recovery mass balances of 115 ± 18% and 60 ± 30% of the Ce were obtained for the pulse dosing and the chronic dosing, respectively. This demonstrated the relevance of our experimental approach that allows for adequately monitoring the fate and impact of a given nanomaterial.
authors.affiliation: 1] CNRS, Aix-Marseille Université, CEREGE UM34, UMR 7330, 13545 Aix en Provence, France [2] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [3] Center for the Environmental Implications of NanoTechnology CEINT, Duke University, Durham, North Carolina 27707, USA.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Auffan
authors.affiliation: 1] CNRS, Aix-Marseille Université, CEREGE UM34, UMR 7330, 13545 Aix en Provence, France [2] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Tella
authors.affiliation: 1] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [2] CNRS, Aix-Marseille Université, CEA Cadarache DSV/IBEB/SBVME, Lab Ecol Microb Rhizosphere & Environ Extrem (LEMiRE), UMR 7265, Saint Paul lez Durance, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Santaella
authors.affiliation: 1] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [2] CNRS, Aix-Marseille Université, CNRS, Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale (IMBE), UMR 7263, Marseille, France.
authors.forename: Lenka
authors.initials: L
authors.lastname: Brousset
authors.affiliation: 1] CNRS, Aix-Marseille Université, CEREGE UM34, UMR 7330, 13545 Aix en Provence, France [2] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Paillès
authors.affiliation: 1] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [2] CNRS, Aix-Marseille Université, CEA Cadarache DSV/IBEB/SBVME, Lab Ecol Microb Rhizosphere & Environ Extrem (LEMiRE), UMR 7265, Saint Paul lez Durance, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Barakat
authors.affiliation: 1] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [2] Center for the Environmental Implications of NanoTechnology CEINT, Duke University, Durham, North Carolina 27707, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Espinasse
authors.affiliation: 1] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [2] CNRS, Aix-Marseille Université, CNRS, Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale (IMBE), UMR 7263, Marseille, France.
authors.forename: Ester
authors.initials: E
authors.lastname: Artells
authors.affiliation: 1] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [2] CNRS, Aix-Marseille Université, CNRS, Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale (IMBE), UMR 7263, Marseille, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Issartel
authors.affiliation: 1] CNRS, Aix-Marseille Université, CEREGE UM34, UMR 7330, 13545 Aix en Provence, France [2] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France.
authors.forename: Armand
authors.initials: A
authors.lastname: Masion
authors.affiliation: 1] CNRS, Aix-Marseille Université, CEREGE UM34, UMR 7330, 13545 Aix en Provence, France [2] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [3] Center for the Environmental Implications of NanoTechnology CEINT, Duke University, Durham, North Carolina 27707, USA.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Rose
authors.affiliation: 1] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [2] Center for the Environmental Implications of NanoTechnology CEINT, Duke University, Durham, North Carolina 27707, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Wiesner
authors.affiliation: 1] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [2] CNRS, Aix-Marseille Université, CEA Cadarache DSV/IBEB/SBVME, Lab Ecol Microb Rhizosphere & Environ Extrem (LEMiRE), UMR 7265, Saint Paul lez Durance, France.
authors.forename: Wafa
authors.initials: W
authors.lastname: Achouak
authors.affiliation: 1] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [2] CNRS, Aix-Marseille Université, CNRS, Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale (IMBE), UMR 7263, Marseille, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Thiéry
authors.affiliation: 1] CNRS, Aix-Marseille Université, CEREGE UM34, UMR 7330, 13545 Aix en Provence, France [2] International Consortium for the Environmental Implications of Nanotechnology iCEINT, CNRS-Duke University, Aix en Provence, France [3] Center for the Environmental Implications of NanoTechnology CEINT, Duke University, Durham, North Carolina 27707, USA.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Bottero
mesh_terms: D001681:Biological Assay / Q000295:instrumentation*; D019149:Bioreactors; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D017753:Ecosystem; D004780:Environment, Controlled; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008422:Materials Testing / Q000295:instrumentation; D053758:Nanoparticles / Q000633:toxicity*; D036103:Nanotechnology / Q000295:instrumentation; D010839:Phytoplankton / Q000187:drug effects / Q000502:physiology*; D018511:Systems Integration; D018675:Toxicity Tests / Q000295:instrumentation*