title: TNF-related Apoptosis-inducing Ligand Delivered by rNDV is a Novel Agent for Cancer Gene Therapy.
abstract: Recombinant Newcastle disease virus (rNDV) as antitumor agent has been shown to be effective for cancer therapy. And TNF-related apoptosis-inducing ligand (TRAIL) also has been demonstrated potentially cancer-therapeutic effects. In this study, we constructed TRAIL delivered by rNDV (rNDV-TRAIL) and investigated whether TRAIL would generate the potential synergistic therapeutic effects with rNDV for cancer therapy. In vitro experiments indicated that TRAIL expressed by rNDV demonstrated good biological activity. TRAIL significantly enhanced inducing apoptosis of rNDV in death receptor expression cancer cell lines. Experiments in malignant melanoma-bearing mice demonstrated that expression of TRAIL delivered by rNDV significantly inhibited the tumor growth and prolonged the survival of treated animals compared to control. In conclusion, oncolytic capacity of rNDV was augmented by TRAIL and the inherent anti-neoplastic properties of NDV were enhanced by the introduction of therapeutic TRAIL gene.
authors.affiliation: Department of Biopharmaceutical Teaching and Research, College of Life Science, Northeast Agricultural University, Mucai street 59, Xiangfang District, Harbin 150030, China.
authors.forename: Fu-Liang
authors.initials: FL
authors.lastname: Bai
authors.affiliation: Department of Biopharmaceutical Teaching and Research, College of Life Science, Northeast Agricultural University, Mucai street 59, Xiangfang District, Harbin 150030, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tian
authors.affiliation: Department of Biopharmaceutical Teaching and Research, College of Life Science, Northeast Agricultural University, Mucai street 59, Xiangfang District, Harbin 150030, China.
authors.forename: Yin-Hang
authors.initials: YH
authors.lastname: Yu
authors.affiliation: Department of Biopharmaceutical Teaching and Research, College of Life Science, Northeast Agricultural University, Mucai street 59, Xiangfang District, Harbin 150030, China.
authors.forename: Jie-Chao
authors.initials: JC
authors.lastname: Yin
authors.affiliation: Department of Biopharmaceutical Teaching and Research, College of Life Science, Northeast Agricultural University, Mucai street 59, Xiangfang District, Harbin 150030, China.
authors.forename: Gui-Ping
authors.initials: GP
authors.lastname: Ren
authors.affiliation: Department of Biopharmaceutical Teaching and Research, College of Life Science, Northeast Agricultural University, Mucai street 59, Xiangfang District, Harbin 150030, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of Biopharmaceutical Teaching and Research, College of Life Science, Northeast Agricultural University, Mucai street 59, Xiangfang District, Harbin 150030, China desganli@163.com.
authors.forename: De-Shan
authors.initials: DS
authors.lastname: Li
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D008546:Melanoma, Experimental / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009522:Newcastle disease virus; D016133:Polymerase Chain Reaction; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000008:administration & dosage*; D023041:Xenograft Model Antitumor Assays
chemical_list: D053221:TNF-Related Apoptosis-Inducing Ligand
keywords: Antitumor drug; Cancer gene therapy; Cancer therapy; Recombinant Newcastle disease virus; TNF-related apoptosis-inducing ligand