title: [Clinical observation of haploidentical-hematopoietic stem cell transplantation combined with human umbilical cord-derived mesenchymal stem cells for severe aplastic anemia-II].
abstract: This study was purposed to investigate the efficacy and safety of haploidentical hematopoietic stem cells (allo-HSCT) transplantation combined with human umbilical cord-derived mesenchymal stem cell infusion (hUC-MSC) for severe aplastic anemia-II (SAA-II). Eight SAA-II patients received haploidentical allo-HSCT, the G-CSF mobilized peripheral hematopoietic stem cells and bone marrow haploidentical hematopoietic stem cells were selected as graft, the human umbilical cord-derived mesenchymal stem cells (hUC-MSC) were infused as the third party. Conditioning regimen consisted of rabbit anti-thymic lymphocytes protein(ATG), cyclophosphamide(CTX) and fludarabine(Flu). For two patients out of 8 SAA-II patients the conditioning regimen was combined with busulfan(BU). The graft versus host disease(GVHD) was prevented with CSA, MTX, ATG, CD25 and mycophenolate mofetil. The results showed that the average number of nucleated cells were 9.13×10(8)/kg, and number of CD34(+)cells were 3.76×10(6)/ kg. All the 8 SAA-II patients achieved hematopoietic reconstitution. The average time of neutrophils count>0.5×10(9)/L was 11.9 days, and average time of Plt level >20×10(9)/L was 14.6 days. The incidence of acute GVHD of I-II grade was 25%, and that of III-IVgrade was 12.5%, the transplantation-related mortality was 25%. It is concluded that haploidentical allo-HSCT combined with umbilical cord MSC infusion is an effective approach to cure SAA.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Xu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Zhou-Yang
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Ya-Mei
authors.initials: YM
authors.lastname: Wu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Yong-Bin
authors.initials: YB
authors.lastname: Cao
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Li
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Yan
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Xue-Liang
authors.initials: XL
authors.lastname: Yang
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing 100048, China.
authors.forename: Wan-Ming
authors.initials: WM
authors.lastname: DA
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, China. E-mail: xiongwuxiao@sohu.com.
authors.forename: Xiao-Xiong
authors.initials: XX
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000628:therapy*; D002648:Child; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D008875:Middle Aged; D019172:Transplantation Conditioning / Q000379:methods; D014184:Transplantation, Homologous; D055815:Young Adult