title: Spermatozoa isolated from cat testes retain their structural integrity as well as a developmental potential after refrigeration for up to 7 days.
abstract: The objective of this study was to compare the efficiency of preservation media for isolated feline testicular spermatozoa as well as the concentrations of bovine serum albumin (BSA) on: (1) the membrane (sperm membrane integrity (SMI)) and DNA integrity of spermatozoa; and (2) the developmental potential of spermatozoa after intracytoplasmic sperm injection (ICSI). Isolated cat spermatozoa were stored in HEPES-M199 medium (HM) or Dulbecco's phosphate-buffered saline (DPBS) at 4°C for up to 7 days. Results indicated that HM maintained a better SMI than DPBS throughout the storage periods (P > 0.05). When spermatozoa were stored in HM supplemented with BSA at different concentrations (4, 8 or 16 mg/ml), SMI obtained from HM containing 8 and 16 mg/ml BSA was higher than with 4 mg/ml BSA (P 0.05). In summary, cat spermatozoa immediately isolated from testicular tissue can be stored as a suspension in basic buffered medium at 4°C for up to 7 days. BSA supplementation into the medium improves membrane integrity of the spermatozoa during cold storage. Testicular spermatozoa stored in HM containing 16 mg/ml BSA retained full in vitro developmental potential after ICSI, similar to that of fresh controls even though DNA integrity had slightly declined.
authors.affiliation: Department of Obstetrics,Gynaecology and Reproduction,Faculty of Veterinary Science,Chulalongkorn University,Bangkok,Thailand.
authors.forename: Sirirak
authors.initials: S
authors.lastname: Buarpung
authors.affiliation: Department of Obstetrics,Gynaecology and Reproduction,Faculty of Veterinary Science,Chulalongkorn University,Bangkok,Thailand.
authors.forename: Theerawat
authors.initials: T
authors.lastname: Tharasanit
authors.affiliation: Department of Obstetrics,Gynaecology and Reproduction,Faculty of Veterinary Science,Chulalongkorn University,Bangkok,Thailand.
authors.forename: Chommanart
authors.initials: C
authors.lastname: Thongkittidilok
authors.affiliation: Department of Reproductive Sciences,Center for Species Survival,Smithsonian Conservation Biology Institute,Washington,District of Columbia,USA.
authors.forename: Pierre
authors.initials: P
authors.lastname: Comizzoli
authors.affiliation: Department of Obstetrics,Gynaecology and Reproduction,Faculty of Veterinary Science,Chulalongkorn University,Bangkok,Thailand.
authors.forename: Mongkol
authors.initials: M
authors.lastname: Techakumphu
mesh_terms: D000818:Animals; D002415:Cats; D002417:Cattle; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D012034:Refrigeration; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020554:Sperm Injections, Intracytoplasmic / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D053625:Sperm Retrieval / Q000662:veterinary; D013094:Spermatozoa / Q000737:chemistry* / Q000166:cytology* / Q000502:physiology; D013737:Testis / Q000166:cytology* / Q000502:physiology
chemical_list: D012710:Serum Albumin, Bovine
keywords: Cold storage; DNA integrity; ICSI; Membrane integrity; Testicular spermatozoa