title: [An effective method of isolation and primary culture of human umbilical arterial endothelial cells].
abstract: OBJECTIVE
  To explore and establish an efficient system for isolation and culture of human umbilical arterial endothelial cells (HUAECs).
  
  
  METHODS
  The human umbilical arteries were perfused and washed by PBS, and then digested by 0.1 g/L type 1 collagenase. Digested cell supernatants were collected, centrifuged, and the re-suspended cells were cultured in a specific medium. The identification of endothelial cells was carried out by observing the cellular morphology, immunostaining the endothelial surface marker CD31 under fluorescent microscopy and examining the capability to form tube-like structure.
  
  
  RESULTS
  The isolated cells grew in a monolayer and exhibited mosaic-like pattern under an inverted phase-contrast microscope. They strongly expressed CD31, an endothelium-specific surface marker, and had an obvious capability to form tube-like structure.
  
  
  CONCLUSIONS
  The method established for isolation of HUAECs in this experiment is simple, rapid and efficient. In addition, the isolated cells are of high purity and high survival.
authors.affiliation: Laboratory of Vascular Biology, Institute of Molecular Medicine, Peking University, Beijing 100871, China.
authors.forename: Yingqing
authors.initials: Y
authors.lastname: Huo
authors.affiliation: Haidian Maternal and Child Health Hospital of Beijing, Beijing 100080, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Haidian Maternal and Child Health Hospital of Beijing, Beijing 100080, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: He
authors.affiliation: Laboratory of Vascular Biology, Institute of Molecular Medicine, Peking University, Beijing 100871, China.
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Han
authors.affiliation: Laboratory of Vascular Biology, Institute of Molecular Medicine, Peking University, Beijing 100871, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Laboratory of Vascular Biology, Institute of Molecular Medicine, Peking University, Beijing 100871, China.
authors.forename: Jincai
authors.initials: J
authors.lastname: Luo
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D061251:Primary Cell Culture / Q000379:methods*; D015203:Reproducibility of Results; D014469:Umbilical Arteries / Q000166:cytology*
chemical_list: D015415:Biomarkers; D019408:Platelet Endothelial Cell Adhesion Molecule-1