title: Measuring the rate constants of Ca²⁺ indicators.
abstract: Fluorescent Ca(2+) indicators are widely used to measure the concentration of free Ca(2+) ([Ca(2+)](free)) in biological processes. To determine the exact kinetics of changes in [Ca(2+)](free) and the processes underlying these changes (e.g., Ca(2+) binding to Ca(2+)-binding proteins), it is necessary to know the exact binding properties of the indicator used. Here, we describe how to determine the binding rate constants (k(on) and k(off)) of Ca(2+) indicators.
authors.affiliation: Department of Neurology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095;
authors.forename: Guido C
authors.initials: GC
authors.lastname: Faas
authors.affiliation: Department of Neurology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095; Department of Physiology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095.
authors.forename: Istvan
authors.initials: I
authors.lastname: Mody
mesh_terms: D002118:Calcium / Q000032:analysis* / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D005456:Fluorescent Dyes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium