title: Anticancer activity of starch/poly[N-(2-hydroxypropyl)methacrylamide]: biomaterial film to treat skin cancer.
abstract: In the present work, films of pHPMA, pHPMAS hybrid, pHPMA-CPT and pHPMAS-CPT hybrid were prepared by solvent casting method and characterized by FT-IR, FT-Raman, XRD and DSC, respectively. The biocompatibility of the prepared pHPMA film and pHPMAS hybrid film were assessed using VERO cell lines and the percentage cell viability was found to be 97.4 and 98.3% for 7.8 μg/ml of the film extracts after 72 h of incubation. The cancer cell viability of the pHPMA-CPT film and pHPMAS-CPT film using MCF7 cell lines at pH 5.5 and 7.4 were found to be 4.9 and 8.6% and 7.7 and 12.3%, respectively. In vitro release of camptothecin from pHPMA-CPT and pHPMAS-CPT films in phosphate-buffered saline solution at pH 5.5 and 7.4 were monitored and analyzed using UV-vis spectrophotometer at λmax of 360 nm.
authors.affiliation: Department of Chemistry, Anna University, Chennai, Tamil Nadu 600 025, India.
authors.forename: Subashree
authors.initials: S
authors.lastname: Mohanraj
authors.affiliation: Department of Chemistry, Anna University, Chennai, Tamil Nadu 600 025, India.
authors.forename: Dhanalakshmi
authors.initials: D
authors.lastname: Murugan
authors.affiliation: Department of Chemistry, Anna University, Chennai, Tamil Nadu 600 025, India.
authors.forename: Aburva
authors.initials: A
authors.lastname: Rengarajan
authors.affiliation: Department of Chemistry, Anna University, Chennai, Tamil Nadu 600 025, India. Electronic address: sheeja@annauniv.edu.
authors.forename: Sheeja
authors.initials: S
authors.lastname: Rajiv
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D002166:Camptothecin / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D012878:Skin Neoplasms / Q000188:drug therapy; D017550:Spectroscopy, Fourier Transform Infrared; D013213:Starch / Q000737:chemistry*; D013818:Thermogravimetry; D014709:Vero Cells
chemical_list: D000178:Acrylamides; D000972:Antineoplastic Agents, Phytogenic; D001672:Biocompatible Materials; D004337:Drug Carriers; D013213:Starch; C032976:N-(2-hydroxypropyl)methacrylamide; D002166:Camptothecin
keywords: Anticancer activity; Biocompatibility; Camptothecin (CPT); Poly[N-(2-hydroxypropyl)methacrylamide] (pHPMA); Poly[N-(2-hydroxypropyl)methacrylamide]-starch hybrid (pHPMAS) film