title: Streptomyces lushanensis sp. nov., a novel actinomycete with anti-cyanobacterial activity.
abstract: Strain JXJ 0135(T), an anti-cyanobacterial actinomycete, was isolated from a soil sample collected from Lushan Mountain, south China, and identified by using polyphasic approach. Phylogenetic analysis of the near-complete 16S rRNA gene sequence indicated that strain JXJ 0135(T) belongs to the genus Streptomyces and exhibited distinct subclade and also highest similarity (98.6%) to Streptomyces scopuliridis RB72(T). The strain developed well-branched substrate and aerial mycelia, and produced spiral spore chains. Spores were elliptical and the spore surface was smooth. The strain contained LL-diaminopimelic acid with whole-cell sugars of mannose, rhamnose, glucose and galactose. Phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides, phosphotidylinositol dimannoside, an unidentified aminophospholipid and an unknown phospholipid. The menaquinones were MK-9(H6) and MK-9(H8). The major components of the fatty acids were anteiso-C15:0, iso-C16:0, anteiso-C17:0, iso-C15:0, C16:0, iso-C17:0 and iso-C14:0. The G+C content was 69.3 mol%. The DNA-DNA hybridization value between JXJ 0135(T) and S. scopuliridis RB72(T) was 41.2±1.4%. On the basis of the polyphasic data, strain JXJ 0135(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces lushanensis sp. nov. is proposed. The type strain is JXJ 0135(T) (=DSM 42121(T)=JCM 19628(T)=KCTC 29261(T)=KACC 17834(T)=NRRL B-24994(T)).
authors.affiliation: 1] Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China [2] College of Life Science, Jiujiang University, Jiujiang, China.
authors.forename: Bing-Huo
authors.initials: BH
authors.lastname: Zhang
authors.affiliation: 1] Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China [2] College of Life Science, Jiujiang University, Jiujiang, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Hongyun Honghe Tobacco (Group) Co., Ltd, Kunming, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Life Science, Jiujiang University, Jiujiang, China.
authors.forename: Han-Quan
authors.initials: HQ
authors.lastname: Li
authors.affiliation: College of Life Science, Jiujiang University, Jiujiang, China.
authors.forename: Jian-Yuan
authors.initials: JY
authors.lastname: Yang
authors.affiliation: Microbial Resource Center, KRIBB, Daejeon, Republic of Korea.
authors.forename: Dong-Jin
authors.initials: DJ
authors.lastname: Park
authors.affiliation: Microbial Resource Center, KRIBB, Daejeon, Republic of Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Shen
authors.affiliation: Hongyun Honghe Tobacco (Group) Co., Ltd, Kunming, China.
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Duan
authors.affiliation: 1] Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China [2] State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001482:Base Composition; D002681:China; D000458:Cyanobacteria / Q000187:drug effects*; D004269:DNA, Bacterial / Q000235:genetics*; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000737:chemistry; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S