title: Changes of costimulatory molecule CD28 on circulating CD8+ T cells correlate with disease pathogenesis of chronic hepatitis B.
abstract: Costimulatory signals are critical for antiviral immunity. The aim of this study was to evaluate the change of costimulatory molecule CD28 on circulating CD8+ T cells in chronic hepatitis B patients (CHB). Seventy CHB patients and fifty-six healthy controls were included, and forty-eight CHB patients were recruited for 52 weeks of longitudinal investigation. The proportions of circulating CD8+CD28+ and CD8+CD28- subpopulations were determined by flow cytometry, and the CD8+CD28+/CD8+CD28- T cells ratio was calculated. Compared with the subpopulation in healthy controls, high proportions of CD8+CD28- subpopulation were observed in CHB patients. Similarly, the CD8+CD28+/CD8+CD28- T cells ratio was significantly decreased in CHB patients compared with healthy controls and correlated significantly with hepatitis B virus (HBV) loads. High proportions of CD8+CD28- subpopulation and low CD8+CD28+/CD8+CD28- T cells ratio were observed in hepatitis B e antigen- (HBeAg-) positive individuals as compared with that in HBeAg-negative subjects. A significant decrease in CD8+CD28- subpopulation, increase in CD8+CD28+ subpopulation, and CD8+CD28+/CD8+CD28- T cells ratio were seen in those patients who received efficient antiviral therapy. Thus, aberrant CD28 expression on circulating CD8+ T cells and the CD8+CD28+/CD8+CD28- T cells ratio reflect the dysregulation of T cell activation and are related to the pathogenesis of chronic HBV infection.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China ; Department of Laboratory Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Xuefen
authors.identifier: 0000-0002-2989-3477
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Haishen
authors.identifier: 0000-0002-9482-5999
authors.initials: H
authors.lastname: Kong
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Qiaoyun
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Yiyin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Yuejiao
authors.initials: Y
authors.lastname: Dong
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ni
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China ; Department of Laboratory Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000328:Adult; D018106:CD28 Antigens / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D019070:Cell Lineage / Q000276:immunology; D005260:Female; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000276:immunology* / Q000472:pathogenicity; D019694:Hepatitis B, Chronic / Q000097:blood / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008137:Longitudinal Studies; D008297:Male
chemical_list: D018106:CD28 Antigens; D006513:Hepatitis B e Antigens