title: Anti-hypertensive effect of radiofrequency renal denervation in spontaneously hypertensive rats.
abstract: OBJECTIVE
  We aimed to investigate the anti-hypertensive effect of radiofrequency (RF) renal denervation (RDN) in an animal model of hypertension.
  
  
  METHODS
  RF energy was delivered to bilateral renal arteries through a 2 Fr catheter with opening abdomen in 8 spontaneously hypertensive rats (SHRs) and 8 Wistar-Kyoto rats (WKYs). Sham operation was performed in other 8 SHRs and 8 WKYs. Blood pressure (BP), heart rate (HR), and urinary norepinephrine excretion were followed up for 3 months. Plasma and renal tissue concentrations of norepinephrine and plasma renin activity were measured 3 months after the procedure. The RDN was confirmed by a decrease in renal tissue norepinephrine.
  
  
  RESULTS
  RF-RDN restrained a spontaneous rise in systolic BP (46 ± 12% increase from 158 ± 8 to 230 ± 14 mmHg vs. 21 ± 18% increase from 165 ± 9 to 197 ± 20 mmHg, p = 0.01) and diastolic BP (55 ± 27% increase from 117 ± 9 to 179 ± 23 mmHg vs. 28 ± 13% increase from 120 ± 7 to 154 ± 13 mmHg, p = 0.04) in SHRs; however, WKYs were not affected. Although there were no changes in HR and systemic norepinephrine, the renal tissue norepinephrine was decreased by RF-RDN in both SHR (302 ± 41 vs. 159 ± 44 ng/g kidney, p<0.01) and WKY (203 ± 33 vs. 145 ± 26 ng/g kidney, p = 0.01). Plasma renin activity was reduced by the RF-RDN only in SHR (35.3 ± 9.5 vs. 21.4 ± 8.6 ng/mL/h, p<0.01).
  
  
  CONCLUSIONS
  RF-RDN demonstrated an anti-hypertensive effect with a reduction of renal tissue norepinephrine and plasma renin activity in SHR.
authors.affiliation: Cardiovascular Division, Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Machino
authors.affiliation: Cardiovascular Division, Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan. Electronic address: n.murakoshi@md.tsukuba.ac.jp.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Murakoshi
authors.affiliation: Cardiovascular Division, Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Sato
authors.affiliation: Cardiovascular Division, Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: DongZhu
authors.initials: D
authors.lastname: Xu
authors.affiliation: Cardiovascular Division, Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Hoshi
authors.affiliation: Cardiovascular Division, Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Taizo
authors.initials: T
authors.lastname: Kimura
authors.affiliation: Cardiovascular Division, Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Aonuma
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000528:radiation effects*; D003714:Denervation / Q000379:methods*; D006339:Heart Rate / Q000528:radiation effects; D007668:Kidney / Q000294:innervation* / Q000528:radiation effects*; D008297:Male; D009638:Norepinephrine / Q000097:blood / Q000652:urine; D061208:Pulsed Radiofrequency Treatment / Q000379:methods*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012083:Renin / Q000097:blood
chemical_list: D012083:Renin; D009638:Norepinephrine
keywords: Endothelin; Norepinephrine; Renal denervation; Renin; Resistant hypertension; SHR