title: Identification of QTLs associated with resistance to Phomopsis pod blight (Diaporthe toxica) in Lupinus albus.
abstract: Phomopsis blight in Lupinus albus is caused by a fungal pathogen, Diaporthe toxica. It can invade all plant parts, leading to plant material becoming toxic to grazing animals, and potentially resulting in lupinosis. Identifying sources of resistance and breeding for resistance remains the best strategy for controlling Phomopsis and reducing lupinosis risks. However, loci associated with resistance to Phomopsis blight have not yet been identified. In this study, quantitative trait locus (QTL) analysis identified genomic regions associated with resistance to Phomopsis pod blight (PPB) using a linkage map of L. albus constructed previously from an F8 recombinant inbred line population derived from a cross between Kiev-Mutant (susceptible to PPB) and P27174 (resistant to PPB). Phenotyping was undertaken using a detached pod assay. In total, we identified eight QTLs for resistance to PPB on linkage group (LG) 3, LG6, LG10, LG12, LG17 and LG27 from different phenotyping environments. However, at least one QTL, QTL-5 on LG10 was consistently detected in both phenotyping environments and accounted for up to 28.2% of the total phenotypic variance. The results of this study showed that the QTL-2 on LG3 interacts epistatically with QTL-5 and QTL-6, which map on LG10 and LG12, respectively.
authors.affiliation: Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University) , Pine Gully Road, Wagga Wagga, NSW 2650 , Australia.
authors.forename: Raymond
authors.initials: R
authors.lastname: Cowley
authors.affiliation: Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University) , Pine Gully Road, Wagga Wagga, NSW 2650 , Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Luckett
authors.affiliation: Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), School of Agricultural & Wine Sciences, Charles Sturt University , Locked Bag 588, Wagga Wagga, NSW 2650 , Australia.
authors.forename: Gavin J
authors.initials: GJ
authors.lastname: Ash
authors.affiliation: Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), School of Agricultural & Wine Sciences, Charles Sturt University , Locked Bag 588, Wagga Wagga, NSW 2650 , Australia.
authors.forename: John D I
authors.initials: JD
authors.lastname: Harper
authors.affiliation: Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), School of Agricultural & Wine Sciences, Charles Sturt University , Locked Bag 588, Wagga Wagga, NSW 2650 , Australia.
authors.forename: Cina A
authors.initials: CA
authors.lastname: Vipin
authors.affiliation: Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University) , Pine Gully Road, Wagga Wagga, NSW 2650 , Australia.
authors.forename: Harsh
authors.initials: H
authors.lastname: Raman
authors.affiliation: Australian Centre for Necrotrophic Fungal Pathogens, Curtin University , Perth , Western Australia.
authors.forename: Simon
authors.initials: S
authors.lastname: Ellwood
keywords: DArT; Rqtl; anamorph Phomopsis leptostromiformis; broad-leaf lupin; multi-phase experiments