title: Highly discriminatory variable-number tandem-repeat markers for genotyping of Trichophyton interdigitale strains.
abstract: Trichophyton interdigitale is the second most frequent cause of superficial fungal infections of various parts of the human body. Studying the population structure and genotype differentiation of T. interdigitale strains may lead to significant improvements in clinical practice. The present study aimed to develop and select suitable variable-number tandem-repeat (VNTR) markers for 92 clinical strains of T. interdigitale. On the basis of an analysis of four VNTR markers, four to eight distinct alleles were detected for each marker. The marker with the highest discriminatory power had eight alleles and a D value of 0.802. The combination of all four markers yielded a D value of 0.969 with 29 distinct multilocus genotypes. VNTR typing revealed the genetic diversity of the strains, identifying three populations according to their colonization sites. A correlation between phenotypic characteristics and multilocus genotypes was observed. Seven patients harbored T. interdigitale strains with different genotypes. Typing of clinical T. interdigitale samples by VNTR markers displayed excellent discriminatory power and 100% reproducibility.
authors.affiliation: Laboratory of Fungal and Parasitic Molecular Biology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Drira
authors.affiliation: Laboratory of Fungal and Parasitic Molecular Biology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Hadrich
authors.affiliation: Laboratory of Fungal and Parasitic Molecular Biology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Sourour
authors.initials: S
authors.lastname: Neji
authors.affiliation: Laboratory Service, UHC-Hedi-Chaker, Sfax, Tunisia.
authors.forename: Nedia
authors.initials: N
authors.lastname: Mahfouth
authors.affiliation: Laboratory of Fungal and Parasitic Molecular Biology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Houaida
authors.initials: H
authors.lastname: Trabelsi
authors.affiliation: Laboratory of Fungal and Parasitic Molecular Biology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Hayet
authors.initials: H
authors.lastname: Sellami
authors.affiliation: Laboratory of Fungal and Parasitic Molecular Biology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Fattouma
authors.initials: F
authors.lastname: Makni
authors.affiliation: Laboratory of Fungal and Parasitic Molecular Biology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia ali.ayadi@rns.tn.
authors.forename: Ali
authors.initials: A
authors.lastname: Ayadi
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D058889:Molecular Typing / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D014005:Tinea / Q000175:diagnosis / Q000382:microbiology*; D014249:Trichophyton / Q000145:classification* / Q000235:genetics*; D055815:Young Adult