title: Direct ethanol production from lignocellulosic sugars and sugarcane bagasse by a recombinant Trichoderma reesei strain HJ48.
abstract: Trichoderma reesei can be considered as a candidate for consolidated bioprocessing (CBP) microorganism. However, its ethanol yield needs to be improved significantly. Here the ethanol production of T. reesei CICC 40360 was improved by genome shuffling while simultaneously enhancing the ethanol resistance. The initial mutant population was generated by nitrosoguanidine treatment of the spores, and an improved population producing more than fivefold ethanol than wild type was obtained by genome shuffling. The results show that the shuffled strain HJ48 can efficiently convert lignocellulosic sugars to ethanol under aerobic conditions. Furthermore, it was able to produce ethanol directly from sugarcane bagasse, demonstrating that the shuffled strain HJ48 is a suitable microorganism for consolidated bioprocessing.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China ; College of Life Science and Technology, Guangxi University, Nanning 530004, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China ; College of Life Science and Technology, Guangxi University, Nanning 530004, China ; National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China ; College of Life Science and Technology, Guangxi University, Nanning 530004, China.
authors.forename: Yutuo
authors.initials: Y
authors.lastname: Wei
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China ; College of Life Science and Technology, Guangxi University, Nanning 530004, China ; National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Zhenchong
authors.initials: Z
authors.lastname: Li
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China ; College of Life Science and Technology, Guangxi University, Nanning 530004, China ; National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China.
authors.forename: Ribo
authors.initials: R
authors.lastname: Huang
mesh_terms: D002482:Cellulose / Q000096:biosynthesis* / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D008031:Lignin / Q000096:biosynthesis* / Q000235:genetics; D031786:Saccharum / Q000235:genetics / Q000378:metabolism*; D014242:Trichoderma / Q000235:genetics / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C027433:bagasse