title: Rabdosia japonica var. glaucocalyx Flavonoids Fraction Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Mice.
abstract: Rabdosia japonica var. glaucocalyx (Maxim.) Hara, belonging to the Labiatae family, is widely used as an anti-inflammatory and antitumor drug for the treatment of different inflammations and cancers. Aim of the Study. To investigate therapeutic effects and possible mechanism of the flavonoids fraction of Rabdosia japonica var. glaucocalyx (Maxim.) Hara (RJFs) in acute lung injury (ALI) mice induced by lipopolysaccharide (LPS). Materials and Methods. Mice were orally administrated with RJFs (6.4, 12.8, and 25.6 mg/kg) per day for 7 days, consecutively, before LPS challenge. Lung specimens and the bronchoalveolar lavage fluid (BALF) were isolated for histopathological examinations and biochemical analysis. The level of complement 3 (C3) in serum was quantified by a sandwich ELISA kit. Results. RJFs significantly attenuated LPS-induced ALI via reducing productions of the level of inflammatory mediators (TNF- α , IL-6, and IL-1 β ), and significantly reduced complement deposition with decreasing the level of C3 in serum, which was exhibited together with the lowered myeloperoxidase (MPO) activity and nitric oxide (NO) and protein concentration in BALF. Conclusions. RJFs significantly attenuate LPS-induced ALI via reducing productions of proinflammatory mediators, decreasing the level of complement, and reducing radicals.
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
authors.forename: Chun-Jun
authors.identifier: 0000-0001-8867-3821
authors.initials: CJ
authors.lastname: Chu
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
authors.forename: Nai-Yu
authors.initials: NY
authors.lastname: Xu
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
authors.forename: Xian-Lun
authors.initials: XL
authors.lastname: Li
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
authors.forename: Long
authors.initials: L
authors.lastname: Xia
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong.
authors.forename: Zhi-Tao
authors.initials: ZT
authors.lastname: Liang
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong.
authors.forename: Zhong-Zhen
authors.initials: ZZ
authors.lastname: Zhao
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China.
authors.forename: Dao-Feng
authors.initials: DF
authors.lastname: Chen