title: Effects of feeding plant-derived agents on the colonization of Campylobacter jejuni in broiler chickens.
abstract: The aim of this work was to test the potential use of plant-derived extracts and compounds to control Campylobacter jejuni in broiler chickens. Over a 7-wk feeding period, birds were fed a commercial diet with or without plant extracts (Acacia decurrens, Eremophila glabra), essential oil [lemon myrtle oil (LMO)], plant secondary compounds [terpinene-4-ol and α-tops (including α-terpineol, cineole, and terpinene-4-ol)], and the antibiotic virginiamycin. Traditional culture and real-time quantitative PCR techniques were used to enumerate the numbers of C. jejuni in chicken fecal and cecal samples. In addition, BW and feed intake were recorded weekly for the calculation of BW gain and feed conversion ratio. The mean log10 counts of C. jejuni were similar (P > 0.05) across treatments. However, significantly lower levels of fecal Campylobacter counts (P < 0.05) were recorded at d 41 for the α-tops treatment by culture methods. No differences (P > 0.05) in BW gain were obtained for dietary supplementation, except for the E. glabra extract, which had a negative impact (P < 0.001) on BW, resulting in sporadic death. Results from this study suggest that supplemental natural compounds used in the current study did not reduce the shedding of C. jejuni to desired levels.
authors.affiliation: School of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD 4343, Australia CSIRO Animal, Food and Health Sciences, Queensland Biosciences Precinct, 306 Carmody Road, St Lucia, QLD 4067, Australia Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Mustafa Kemal University, Hatay, Turkey 31100 ckurekci@hotmail.com.
authors.forename: Cemil
authors.initials: C
authors.lastname: Kurekci
authors.affiliation: School of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD 4343, Australia.
authors.forename: Rafat
authors.initials: R
authors.lastname: Al Jassim
authors.affiliation: School of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD 4343, Australia.
authors.forename: Errol
authors.initials: E
authors.lastname: Hassan
authors.affiliation: CSIRO Animal, Food and Health Sciences, Queensland Biosciences Precinct, 306 Carmody Road, St Lucia, QLD 4067, Australia.
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Bishop-Hurley
authors.affiliation: CSIRO Animal, Food and Health Sciences, Queensland Biosciences Precinct, 306 Carmody Road, St Lucia, QLD 4067, Australia.
authors.forename: Jagadish
authors.initials: J
authors.lastname: Padmanabha
authors.affiliation: CSIRO Animal, Food and Health Sciences, Queensland Biosciences Precinct, 306 Carmody Road, St Lucia, QLD 4067, Australia.
authors.forename: Christopher S
authors.initials: CS
authors.lastname: McSweeney
mesh_terms: D000045:Acacia / Q000737:chemistry; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D057138:Bacterial Shedding / Q000187:drug effects; D002169:Campylobacter Infections / Q000517:prevention & control / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000187:drug effects*; D002432:Cecum / Q000382:microbiology; D002645:Chickens / Q000254:growth & development; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D041621:Eremophila Plant / Q000737:chemistry; D005243:Feces / Q000382:microbiology; D008297:Male; D010936:Plant Extracts; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D011897:Random Allocation; D014769:Virginiamycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D014769:Virginiamycin
keywords: Campylobacter jejuni; chicken; essential oil; plant extract