title: Capillary coated with graphene oxide as stationary phase for the separation of brucine and strychnine by capillary electrophoresis.
abstract: A new capillary electrophoresis (CE) method was developed by using graphene oxide (GO) as a stationary phase for the separation of brucine and strychnine. The separation performance, reproducibility and stability of GO-coated capillary were investigated for the analysis of brucine and strychnine. After optimization of the separation conditions, a phosphate solution (40 mM, pH 7.0) containing 25% (v/v) acetonitrile was selected as the running buffer. Compared with uncoated capillary, higher separation efficiency was achieved by GO-coated capillary as a result of the increasing interactions between the analytes and the stationary phase of capillary. The linear ranges of these two alkaloids were 4.0-100.0 μg mL(-1) with a satisfied correlation coefficients (R > 0.9994), and this novel method provided an efficient separation of brucine and strychnine as well as a good reproducibility and stability. Finally, the developed method was successfully applied for the determination of these two alkaloids in a pharmaceutical formulation of traditional Chinese medicines.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, PR China yensh@cnu.edu.cn.
authors.forename: Nengsheng
authors.initials: N
authors.lastname: Ye
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, PR China.
authors.forename: Chong
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, PR China.
authors.forename: Jichao
authors.initials: J
authors.lastname: Ma