title: [Detection and analysis of Toll-like receptors and DC-SIGN expression in peripheral blood mononuclear cells from patients with chronic urticaria].
abstract: OBJECTIVE
  To detect the expressions of Toll-like receptor 2 (TLR2), TLR7, TLR9 and dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) mRNA and protein in peripheral blood mononuclear cells (PBMCs) from patients with chronic urticaria (CU).
  
  
  METHODS
  PBMCs were isolated from 20 patients with CU and 20 normal controls. The expressions of TLR2, TLR7, TLR9 and DC-SIGN mRNA were detected by real-time fluorescent quantitative PCR and the expressions of their proteins were detected by flow cytometry.
  
  
  RESULTS
  The expression of DC-SIGN mRNA in PBMCs from patients with CU was significantly lower than that from normal controls (P<0.01). The expression of TLR2 protein was significantly higher (P<0.05), but the expression of DC-SIGN protein was significantly lower (P<0.05) in CU patients than those in normal controls.
  
  
  CONCLUSIONS
  The expression of DC-SIGN decreases and that of TLR2 protein increases in patients with CU.
authors.affiliation: No.306 Clinical College of PLA, Anhui Medical University, Beijing 100101, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Dermatology, No.306 Hospital of PLA, Beijing 100101, China.
authors.forename: Junlian
authors.initials: J
authors.lastname: Liu
authors.affiliation: Center for Special Medicine and Experimental Research, No.306 Hospital of PLA, Beijing 100101, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Song
authors.affiliation: Center for Special Medicine and Experimental Research, No.306 Hospital of PLA, Beijing 100101, China.
authors.forename: Bingxin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Center for Special Medicine and Experimental Research, No.306 Hospital of PLA, Beijing 100101, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Tan
authors.affiliation: Center for Special Medicine and Experimental Research, No.306 Hospital of PLA, Beijing 100101, China.
authors.forename: Shaoyan
authors.initials: S
authors.lastname: Si
mesh_terms: D000328:Adult; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D002908:Chronic Disease; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D051199:Toll-Like Receptor 7 / Q000235:genetics / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000235:genetics / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics* / Q000378:metabolism; D014581:Urticaria / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D051195:Toll-Like Receptor 2; D051199:Toll-Like Receptor 7; D051217:Toll-Like Receptor 9; D051193:Toll-Like Receptors; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C495348:TLR7 protein, human