title: [Conjunctival impression cytology in patients with normal and impaired OSDI scores].
abstract: OBJECTIVE
  To describe goblet cell density and Nelson grading in different areas of the ocular surface using conjunctival impression cytology (CIC) among patients with normal and impaired Ocular Surface Disease Index (OSDI) scores.
  
  
  METHODS
  Patients (n=166) under assessment for dry eye were recruited between 2011 and 2012 and classified according to the OSDI score in 4 categories (normal and impaired). Cytological study (CIC plus Papanicolaou staining) using the Nelson grading system, with modifications in staging, and goblet cell counting were performed on the nasal, temporal, inferior, and superior bulbar conjunctival surfaces.
  
  
  RESULTS
  Nelson grading was significantly higher in patients with a severely impaired OSDI score (1.41±0.14) compared to normal patients (0.86±0.09) (P<.01). Goblet cell density was significantly reduced in patients with a severely impaired OSDI score (310.24±56.24 cells per sample) compared with normal subjects (497.31±50.07 cells per sample) (P<.001). Compared with the photoexposed bulbar conjunctiva, goblet cell density on the non-photoexposed conjunctiva was significantly higher both in patients with mild (P<.01) and moderate (P<.001) OSDI scores.
  
  
  CONCLUSIONS
  Patients with severely impaired OSDI scores have less goblet cells and a higher Nelson grade. Goblet cells are more abundant on the non-photoexposed conjunctiva.
authors.affiliation: Fundación Oftalmológica Los Andes (FOLA), Santiago, Chile.
authors.forename: F
authors.initials: F
authors.lastname: Zuazo
authors.affiliation: Fundación Oftalmológica Los Andes (FOLA), Santiago, Chile.
authors.forename: D
authors.initials: D
authors.lastname: López-Ponce
authors.affiliation: Fundación Oftalmológica Los Andes (FOLA), Santiago, Chile.
authors.forename: D
authors.initials: D
authors.lastname: Salinas-Toro
authors.affiliation: Fundación Oftalmológica Los Andes (FOLA), Santiago, Chile.
authors.forename: F
authors.initials: F
authors.lastname: Valenzuela
authors.affiliation: Programa de Biología Celular y Molecular, Facultad de Medicina-ICBM, Universidad de Chile, Santiago, Chile.
authors.forename: J
authors.initials: J
authors.lastname: Sans-Puroja
authors.affiliation: Fundación Oftalmológica Los Andes (FOLA), Santiago, Chile.
authors.forename: M
authors.initials: M
authors.lastname: Srur
authors.affiliation: Programa de Biología Celular y Molecular, Facultad de Medicina-ICBM, Universidad de Chile, Santiago, Chile.
authors.forename: R O
authors.initials: RO
authors.lastname: López-Solís
authors.affiliation: Fundación Oftalmológica Los Andes (FOLA), Santiago, Chile. Electronic address: ltraipe@gmail.com.
authors.forename: L
authors.initials: L
authors.lastname: Traipe-Castro
mesh_terms: D003228:Conjunctiva / Q000166:cytology / Q000473:pathology*; D003584:Cytological Techniques; D015352:Dry Eye Syndromes / Q000473:pathology*; D005260:Female; D020397:Goblet Cells; D006801:Humans; D008297:Male; D011446:Prospective Studies; D012016:Reference Values
keywords: Citología de impresión conjuntival; Conjunctival impression cytology; Células caliciformes; Dry eye; Goblet cells; Graduación de Nelson; Nelson grading; OSDI; Ojo seco