title: Dicentric chromosome aberration analysis using giemsa and centromere specific fluorescence in-situ hybridization for biological dosimetry: An inter- and intra-laboratory comparison in Indian laboratories.
abstract: To facilitate efficient handling of large samples, an attempt towards networking of laboratories in India for biological dosimetry was carried out. Human peripheral blood samples were exposed to (60)Co γ-radiation for ten different doses (0-5Gy) at a dose rate of 0.7 and 2Gy/min. The chromosomal aberrations (CA) were scored in Giemsa-stained and fluorescence in-situ hybridization with centromere-specific probes. No significant difference (p>0.05) was observed in the CA yield for given doses except 4 and 5Gy, between the laboratories, among the scorers and also staining methods adapted suggest the reliability and validates the inter-lab comparisons exercise for triage applications.
authors.affiliation: Department of Human Genetics, College of Biomedical Sciences Technology and Research, Sri Ramachandra University, Porur, Chennai 600116, Tamil Nadu, India. Electronic address: bhavanigenetics@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Bhavani
authors.affiliation: Department of Human Genetics, College of Biomedical Sciences Technology and Research, Sri Ramachandra University, Porur, Chennai 600116, Tamil Nadu, India; Chemical Radioprotector and Radiation Dosimetry Research Group, Institute of Nuclear Medicine and Allied Sciences, Brig Mazumdar Road, DRDO, Timarpur, New Delhi 110054, India. Electronic address: tamizh096@gmail.co.in.
authors.forename: G
authors.initials: G
authors.lastname: Tamizh Selvan
authors.affiliation: Department of Human Genetics, College of Biomedical Sciences Technology and Research, Sri Ramachandra University, Porur, Chennai 600116, Tamil Nadu, India. Electronic address: pre_singh@yahoo.co.in.
authors.forename: Harpreet
authors.initials: H
authors.lastname: Kaur
authors.affiliation: Chemical Radioprotector and Radiation Dosimetry Research Group, Institute of Nuclear Medicine and Allied Sciences, Brig Mazumdar Road, DRDO, Timarpur, New Delhi 110054, India. Electronic address: adhikari_56@yahoo.co.in.
authors.forename: J S
authors.initials: JS
authors.lastname: Adhikari
authors.affiliation: Department of Human Genetics, College of Biomedical Sciences Technology and Research, Sri Ramachandra University, Porur, Chennai 600116, Tamil Nadu, India. Electronic address: karthivi@yahoo.com.
authors.forename: J
authors.initials: J
authors.lastname: Vijayalakshmi
authors.affiliation: Department of Human Genetics, College of Biomedical Sciences Technology and Research, Sri Ramachandra University, Porur, Chennai 600116, Tamil Nadu, India. Electronic address: venkip@yahoo.com.
authors.forename: P
authors.initials: P
authors.lastname: Venkatachalam
authors.affiliation: Chemical Radioprotector and Radiation Dosimetry Research Group, Institute of Nuclear Medicine and Allied Sciences, Brig Mazumdar Road, DRDO, Timarpur, New Delhi 110054, India. Electronic address: nkcinmas@rediffmail.com.
authors.forename: N K
authors.initials: NK
authors.lastname: Chaudhury
mesh_terms: D001399:Azure Stains / Q000737:chemistry; D001681:Biological Assay / Q000379:methods*; D002478:Cells, Cultured; D002503:Centromere / Q000235:genetics* / Q000528:radiation effects*; D002869:Chromosome Aberrations / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007194:India; D008214:Lymphocytes / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage; D011874:Radiometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001399:Azure Stains
keywords: Biodosimetry; Dicentric chromosome; FISH and Dose‐response curve