title: Regulatory B cells in mouse models of intestinal inflammation.
abstract: Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory condition with increasing incidence and prevalence around the world. Although B cells had generally been believed to play a pathogenic role in IBD due to the production of autoantibodies, a growing body of evidence from mouse models suggests the coexistence of pathogenic B cells and regulatory B cells, termed Breg, in this disorder. Since some unique techniques are required to closely study the Breg in gut-associated lymphoid tissues (GALT), we herein describe how to induce colitis in mice and how to analyze the phenotype and function of GALT-specific Breg.
authors.affiliation: Molecular Pathology Unit, Massachusetts General Hospital, 149 CNY-6024, 13th Street, Charlestown, MA, 02114, USA.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nishida
authors.forename: Cindy W
authors.initials: CW
authors.lastname: Lau
authors.forename: Emiko
authors.initials: E
authors.lastname: Mizoguchi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mizoguchi
mesh_terms: D000818:Animals; D060151:B-Lymphocytes, Regulatory / Q000276:immunology* / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation / Q000379:methods; D003092:Colitis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D003106:Colon / Q000166:cytology / Q000276:immunology / Q000473:pathology*; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology / Q000473:pathology; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D016207:Cytokines; D016264:Dextran Sulfate