title: Porous chitosan scaffolds with surface micropatterning and inner porosity and their effects on Schwann cells.
abstract: Chitosan is found to promote the regeneration of peripheral nerve system in our previous studies, whereas the regeneration speed is not satisfied with clinical request. Micropatterning could promote cell orientation and growth, however, the effect of porous chitosan micropatterning on nerve regeneration is rarely reported. In this study, the porous chitosan micropatterning with surface ridge/groove and inner porosity structure was fabricated using a combination of micromodeling and lyophilization method. The morphology and stability of the prepared chitosan micropatterning were evaluated, the regulation of Schwann cells behavior by chitosan micropatterning was evaluated. The results showed that the chitosan micropatterning displayed stripe-like structure with a clear and complete edge. The micropatterning with 30/30 μm was more stable than 20/20 μm sample. Schwann cells on chitosan micropatterning showed orientation adhesion and began to grow along a certain direction after culture for 2 h, and displayed the minimal orientation angle and the largest length/width ratio on 30/30 μm micropatterning after further culture for 3 d and 5 d, indicating the most obvious cell orientation. Moreover, the secretion of nerve growth factor (NGF) demonstrated that the micropatterned chitosan had no negative influence on the physiological function of Schwann cells. Thus, the results indicate that the porous chitosan micropatterning can regulate Schwann cell growth well, which may have potential application in nerve regeneration. The study provides an important basis for constructing porous nerve conduit with micropatterning structure in the inner wall.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 226001 Nantong, PR China; The Neural Regeneration Co-innovation Center of Jiangsu Province, 226001 Nantong, PR China.
authors.forename: Guicai
authors.initials: G
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 226001 Nantong, PR China; The Neural Regeneration Co-innovation Center of Jiangsu Province, 226001 Nantong, PR China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Blvd, Winston-Salem, NC 27157, USA; The Neural Regeneration Co-innovation Center of Jiangsu Province, 226001 Nantong, PR China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 226001 Nantong, PR China; The Neural Regeneration Co-innovation Center of Jiangsu Province, 226001 Nantong, PR China.
authors.forename: Luzhong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 226001 Nantong, PR China; The Neural Regeneration Co-innovation Center of Jiangsu Province, 226001 Nantong, PR China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 226001 Nantong, PR China; The Neural Regeneration Co-innovation Center of Jiangsu Province, 226001 Nantong, PR China.
authors.forename: Maorong
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 226001 Nantong, PR China; The Neural Regeneration Co-innovation Center of Jiangsu Province, 226001 Nantong, PR China. Electronic address: nervegu@ntu.edu.cn.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 226001 Nantong, PR China; The Neural Regeneration Co-innovation Center of Jiangsu Province, 226001 Nantong, PR China. Electronic address: yangym@ntu.edu.cn.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D020932:Nerve Growth Factor / Q000378:metabolism; D016062:Porosity; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D004129:Dimethylpolysiloxanes; D048271:Chitosan; D020932:Nerve Growth Factor
keywords: Chitosan; Lyophilization; Micromodeling; Micropatterning; Peripheral nerve regeneration; Schwann cell