title: Lipid metabolism disturbances contribute to insulin resistance and decrease insulin sensitivity by malathion exposure in Wistar rat.
abstract: Several studies showed that organophosphorus pesticides disturb glucose homeostasis and can increase incidence of metabolic disorders and diabetes via insulin resistance. The current study investigates the influence of malathion on glucose metabolism regulation, in vivo, during subchronic exposure. Malathion was administered orally (200 mg/kg), once a day for 28 consecutive days. Plasma glucose, insulin and Glycated hemoglobin levels were significantly increased while hepatic glycogen content was decreased in intoxicated animals compared with the control group. Furthermore, there was a significant disturbance of lipid content in subchronic treated and post-treated rats deprived of malathion for one month. In addition, we used the homeostasis model assessment (HOMA) to assess insulin resistance (HOMA-IR) and pancreatic β-cell function (HOMA-β). Our results show that malathion increases insulin resistance biomarkers and decreases insulin sensitivity indices. Statistical analysis demonstrates that there was a positive and strong significant correlation between insulin level and insulin resistance indices, HOMA-IR, HOMA-β. Similarly, a negative and significant correlation was also found between insulin level and insulin sensitivity indices. For the first time, we demonstrate that malathion induces insulin resistance in vivo using homeostasis model assessment and these changes were detectable one month after the end of exposure. To explain insulin resistance induced by malathion we focus on lipid metabolism disturbances and their interaction with many proteins involved in insulin signaling pathways.
authors.affiliation: Laboratoire de physiologie des agressions: études métaboliques et endocriniens, Département de biologie, Faculté des sciences de Tunis, Université El-Manar , Tunis , Tunisie and.
authors.forename: Mohamed Montassar
authors.initials: MM
authors.lastname: Lasram
authors.forename: Kahena
authors.initials: K
authors.lastname: Bouzid
authors.forename: Ines Bini
authors.initials: IB
authors.lastname: Douib
authors.forename: Alya
authors.initials: A
authors.lastname: Annabi
authors.forename: Naziha
authors.initials: N
authors.lastname: El Elj
authors.forename: Saloua
authors.initials: S
authors.lastname: El Fazaa
authors.forename: Jaouida
authors.initials: J
authors.lastname: Abdelmoula
authors.forename: Najoua
authors.initials: N
authors.lastname: Gharbi
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D006442:Glycated Hemoglobin / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008294:Malathion / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin; D007306:Insecticides; D007328:Insulin; D008294:Malathion
keywords: Dyslipidemia; glucose metabolism; insulin resistance; malathion