title: Selective expression of Narp in primary nociceptive neurons: role in microglia/macrophage activation following nerve injury.
abstract: Neuronal activity regulated pentraxin (Narp) is a secreted protein implicated in regulating synaptic plasticity via its association with the extracellular surface of AMPA receptors. We found robust Narp immunostaining in dorsal root ganglia (DRG) that is largely restricted to small diameter neurons, and in the superficial layers of the dorsal horn of the spinal cord. In double staining studies of DRG, we found that Narp is expressed in both IB4- and CGRP-positive neurons, markers of distinct populations of nociceptive neurons. Although a panel of standard pain behavioral assays were unaffected by Narp deletion, we found that Narp knockout mice displayed an exaggerated microglia/macrophage response in the dorsal horn of the spinal cord to sciatic nerve transection 3days after surgery compared with wild type mice. As other members of the pentraxin family have been implicated in regulating innate immunity, these findings suggest that Narp, and perhaps other neuronal pentraxins, also regulate inflammation in the nervous system.
authors.affiliation: Department of Neuroscience, Johns Hopkins University, United States.
authors.forename: M
authors.initials: M
authors.lastname: Miskimon
authors.affiliation: Department of Psychiatry, Johns Hopkins University, United States.
authors.forename: S
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Psychiatry, Johns Hopkins University, United States.
authors.forename: J J
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: Department of Neurosurgery, Johns Hopkins University, United States.
authors.forename: M
authors.initials: M
authors.lastname: Ringkamp
authors.affiliation: Department of Neuroscience, Johns Hopkins University, United States.
authors.forename: M A
authors.initials: MA
authors.lastname: Wilson
authors.affiliation: NIDCD, NIH, Johns Hopkins University, United States.
authors.forename: R S
authors.initials: RS
authors.lastname: Petralia
authors.affiliation: Department of Neuroscience, Johns Hopkins University, United States.
authors.forename: X
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Neuroscience, Johns Hopkins University, United States.
authors.forename: P F
authors.initials: PF
authors.lastname: Worley
authors.affiliation: Department of Neuroscience, Johns Hopkins University, United States; Department of Psychiatry, Johns Hopkins University, United States.
authors.forename: J M
authors.initials: JM
authors.lastname: Baraban
authors.affiliation: Department of Neuroscience, Johns Hopkins University, United States; Department of Psychiatry, Johns Hopkins University, United States; Laboratory of Origin, United States. Electronic address: imreti@jhmi.edu.
authors.forename: I M
authors.initials: IM
authors.lastname: Reti
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000235:genetics / Q000276:immunology*; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000276:immunology; D015870:Gene Expression / Q000276:immunology; D006930:Hyperalgesia / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017628:Microglia / Q000166:cytology / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000276:immunology*; D009619:Nociceptors / Q000276:immunology*; D020671:Posterior Horn Cells / Q000166:cytology / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019051:Rhizotomy; D020426:Sciatic Neuropathy / Q000276:immunology / Q000473:pathology; D011984:Sensory Receptor Cells / Q000166:cytology / Q000276:immunology*; D013979:Tibial Nerve / Q000276:immunology / Q000293:injuries; D020429:Tibial Neuropathy / Q000276:immunology* / Q000473:pathology; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D009419:Nerve Tissue Proteins; D000099153:Pentraxins
keywords: AMPA; CGRP; Dorsal root ganglion; Inflammation; Pain; Pentraxin