title: [Preparation of total alkaloids from Sophora alopecuroides freeze-dried powders and investigation of its antitumor effects].
abstract: OBJECTIVE
  To investigate molding technology of total alkaloids from Sophora alopecuroides freeze-dried powders and observe its inhibition effects on liver transplantation tumor in mice.
  
  
  METHODS
  With color, clarity, water-soluble and formability as indexes, single factor tests were adopted to screen type and amount of filler, the concentration of total alkaloids in drug liquid, pH in order to determine optimum prescription of total alkaloids from S. alopecuroides freeze-dried powders, the lowest melting point was determined and freeze drying curve was drafted. Mice hepatoma H22 ascites tumor strain was sterile inoculated in right axillary subcutaneous of mice, and antitumor effect of total alkaloids from S. alopecuroides freeze-dried powders on liver transplantation tumor H22 in mice.
  
  
  RESULTS
  When selected 80 g x L(-1) as mannitol as filler, the concentration of total alkaloids in drug liquid was 25 g x L(-1) and pH 6.5-7.5, freeze-dried effect was optimum with fast reconstitute speed. Average inhibition rate of the big (120 mg x kg(-1)) and medium (60 mg x kg(-1)) dose group of total alkaloids from S. alopecuroides freeze-dried powders on liver transplantation tumor H22 in mice were 56.08% and 35.49%, respectively.
  
  
  CONCLUSIONS
  Preparation technology was reasonable, reproducible and stable, total alkaloids from S. alopecuroides freeze-dried powders had significant antitumor effect and showed a dose-effect relationship.
authors.forename: Jia-Fu
authors.initials: JF
authors.lastname: Yan
authors.forename: Yan
authors.initials: Y
authors.lastname: Tong
authors.forename: Chun
authors.initials: C
authors.lastname: Pu
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Wang
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003116:Color; D005612:Freeze Drying; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D051379:Mice; D011208:Powders / Q000737:chemistry; D029909:Sophora / Q000737:chemistry*; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D011208:Powders