title: [Effects of leukotriene D4 on proliferation and migration of lung epithelial A549 cells in vitro].
abstract: To investigate the effects of cysteinyl leukotriene (CysLT) receptor agonist leukotriene D4 (LTD4) on proliferation and migration in lung epithelial A549 cells.
  
  
  
  The expression of CysLT1 receptor and CysLT2 receptor was determined by immunofluoresence staining in A549 cells. A549 cells were treated with LTD4 (0.01-100 nmol/L) for 24-72 h. Cell viability was detected by MTT reduction assay. Cell migration was determined by modified scratch and healing model.
  
  
  
  In A549 cells, CysLT1 receptor and CysLT2 receptor were mainly expressed in the cytoplasm, membrane and few in the nuclei. The treatment of LTD4 (0.01-100 nmol/L) for 24-72 h caused no effect on cell viability (Ps>0.05); when A549 cells were treated with 100 nmol/L LTD4 for 24, 48 and 72 h the cell viability was (103.00±4.46)%，(107.00±9.45)% and (105.00±9.02)% of control, respectively (Ps>0.05). The migration rate of A549 cells after scratching during the first 24 h was markedly greater than that during the second and third 24 h in the same concentration groups; however, no significant difference in migration rate was noticed when the cells were treated with different concentrations of LTD4 (0.01-100 nmol/L)(Ps>0.05). The migration of A549 cells was 1.15-fold, 1.21-fold and 1.06-fold of that of control when the cells were treated with 100 nmol/L LTD4 for 24, 48 and 72 h, respectively (Ps>0.05).
  
  
  
  The proliferation and migration of A549 cells are not changed when treated with 0.01-100 nmol LTD4 for up to 72h.
authors.affiliation: Department of Pharmacology， Zhejiang University School of Medicine， Hangzhou 310058， China; Department of Pharmacy， the Childrens Hospital， Zhejiang University School of Medicine， Hangzhou 310003， China.
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Han
authors.affiliation: Department of Pharmacology， Zhejiang University School of Medicine， Hangzhou 310058， China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology， Zhejiang University School of Medicine， Hangzhou 310058， China.
authors.forename: Zhi-xian
authors.initials: ZX
authors.lastname: Liu
authors.affiliation: Department of Pharmacology， Zhejiang University School of Medicine， Hangzhou 310058， China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Pharmacology， Zhejiang University School of Medicine， Hangzhou 310058， China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology， Zhejiang University School of Medicine， Hangzhou 310058， China.
authors.forename: San-hua
authors.initials: SH
authors.lastname: Fang
authors.affiliation: Department of Pharmacology， Zhejiang University School of Medicine， Hangzhou 310058， China.
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology， Zhejiang University School of Medicine， Hangzhou 310058， China.
authors.forename: Er-qing
authors.initials: EQ
authors.lastname: Wei
authors.affiliation: Department of Pharmacology， Zhejiang University School of Medicine， Hangzhou 310058， China.
authors.forename: Yun-bi
authors.initials: YB
authors.lastname: Lu
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D017998:Leukotriene D4 / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000166:cytology*
chemical_list: D017998:Leukotriene D4