title: Enzymatic hydrolysis of beech wood lignocellulose at high solid contents and its utilization as substrate for the production of biobutanol and dicarboxylic acids.
abstract: The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing the synergetic activity of cellulolytic enzymes at solid concentrations of 9%, 17% and 23% (w/w) of crude Organosolv cellulose, glucose concentrations of 57, 113 and 152 g L(-1) are reached. However, the glucose yield decreases from 0.81 to 0.72 g g(-1) at 17% (w/w). Optimal conditions for hydrolysis scale-up under minimal enzyme addition are identified. As result, at 23% (w/w) crude cellulose the glucose yield increases from 0.29 to 0.49 g g(-1). As proof of its applicability, biobutanol, succinic and itaconic acid are produced with the crude hydrolysate. The potential of the substrate is proven e.g. by a high butanol yield of 0.33 g g(-1).
authors.affiliation: University of Kaiserslautern, Institute of Bioprocess Engineering, Kaiserslautern, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Tippkötter
authors.affiliation: University of Kaiserslautern, Institute of Bioprocess Engineering, Kaiserslautern, Germany.
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Duwe
authors.affiliation: University of Kaiserslautern, Institute of Bioprocess Engineering, Kaiserslautern, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wiesen
authors.affiliation: University of Kaiserslautern, Institute of Bioprocess Engineering, Kaiserslautern, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Sieker
authors.affiliation: University of Kaiserslautern, Institute of Bioprocess Engineering, Kaiserslautern, Germany. Electronic address: ulber@mv.uni-kl.de.
authors.forename: Roland
authors.initials: R
authors.lastname: Ulber
mesh_terms: D056804:Biofuels; D000440:Butanols / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism; D003998:Dicarboxylic Acids / Q000378:metabolism*; D029964:Fagus / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D014934:Wood / Q000378:metabolism*; D014994:Xylose / Q000032:analysis
chemical_list: D056804:Biofuels; D000440:Butanols; D003998:Dicarboxylic Acids; C036909:lignocellulose; D008031:Lignin; D014994:Xylose; D002480:Cellulase; D005947:Glucose
keywords: Biobutanol; Enzymatic hydrolysis; Itaconic acid; Lignocellulose; Succinic acid