title: Targeted lipid analysis of haemolytic mycelial extracts of Aspergillus niger.
abstract: Ethanolic extracts of mycelia from Aspergillus niger (strain N402) grown in liquid media were observed to have haemolytic activity on bovine erythrocytes. This haemolytic activity decreased significantly during the time of growth (1-3 days). Moreover, when A. niger was grown on carbon-deprived medium, the efficiency of this haemolytic activity in the ethanolic extracts was much lower than when grown in carbon-enriched medium, and became almost undetectable after 3 days of growth in carbon-deprived medium. The lipid composition of these ethanolic extracts was analysed by liquid chromatography-electrospray ionisation tandem mass spectrometry. This haemolytic activity can be mainly linked to the relative levels of the molar ratios of the unsaturated fatty acids and lysophosphatidylcholines.
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia. marusa.novak@bf.uni-lj.si.
authors.forename: Maruša
authors.initials: M
authors.lastname: Novak
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia. kristina.sepcic@bf.uni-lj.si.
authors.forename: Kristina
authors.initials: K
authors.lastname: Sepčić
authors.affiliation: National Institute of Chemistry, 1000 Ljubljana, Slovenia. nada.krasevec@ki.si.
authors.forename: Nada
authors.initials: N
authors.lastname: Kraševec
authors.affiliation: Department of Molecular and Biomedical Sciences, Jožef Stefan Institute, 1000 Ljubljana, Slovenia. igor.krizaj@ijs.si.
authors.forename: Igor
authors.initials: I
authors.lastname: Križaj
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia. peter.macek@bf.uni-lj.si.
authors.forename: Peter
authors.initials: P
authors.lastname: Maček
authors.affiliation: National Institute of Chemistry, 1000 Ljubljana, Slovenia. gregor.anderluh@ki.si.
authors.forename: Gregor
authors.initials: G
authors.lastname: Anderluh
authors.affiliation: Department of Physics, Bioorganic Chemistry Laboratory, University of Trento, Via Sommarive 14, I-38123 Povo-Trento, Italy. graziano.guella@unitn.it.
authors.forename: Graziano
authors.initials: G
authors.lastname: Guella
authors.affiliation: Department of Physics, Bioorganic Chemistry Laboratory, University of Trento, Via Sommarive 14, I-38123 Povo-Trento, Italy. ines.mancini@unitn.it.
authors.forename: Ines
authors.initials: I
authors.lastname: Mancini
mesh_terms: D000818:Animals; D001234:Aspergillus niger / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D003470:Culture Media; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D006461:Hemolysis; D053878:Hemolytic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D050356:Lipid Metabolism; D008244:Lysophosphatidylcholines / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D025282:Mycelium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D003470:Culture Media; D005231:Fatty Acids, Unsaturated; D053878:Hemolytic Agents; D008244:Lysophosphatidylcholines