title: Production of manganese peroxidase and laccase in a solid-state bioreactor and modeling of enzyme production kinetics.
abstract: Lignin-modifying enzymes have various promising applications such as biobleaching, biopulping, the functionalization of lignocellulosic materials, the modification of wood fibers, the remediation of contaminated soil and effluents, as well as improvement of the enzymatic hydrolysis of lignocellulosic substrates. In this study, the production of laccase and manganese peroxidase (MnP) in solid-state cultivation was examined. Oat husks were used as an inexpensive substrate for the white-rot fungus Cerrena unicolor PM170798 (FBCC 387). The addition of a fines fraction (consisting of oat flour and finely ground husks) enhanced MnP production fivefold and laccase production almost threefold. The enzyme production was studied first on a 100 g scale, and the cultivation experiments were then repeated at a larger laboratory-scale (4 kg) in a solid-state bioreactor. High enzyme activity levels were obtained (MnP: 340 nkat g(-1) DM, laccase: 470 nkat g(-1) DM). In addition, the correlation between the CO2 evolution rate and enzyme production was mathematically modeled from the bioreactor experimental data. The model parameters could be used to predict enzyme production.
authors.affiliation: Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, PO Box 16100, 00076, Espoo, Finland, ulla.moilanen@helsinki.fi.
authors.forename: Ulla
authors.initials: U
authors.lastname: Moilanen
authors.forename: Erika
authors.initials: E
authors.lastname: Winquist
authors.forename: Tuomas
authors.initials: T
authors.lastname: Mattila
authors.forename: Annele
authors.initials: A
authors.lastname: Hatakka
authors.forename: Tero
authors.initials: T
authors.lastname: Eerikäinen
mesh_terms: D018554:Avena / Q000378:metabolism; D019149:Bioreactors; D007700:Kinetics; D042845:Laccase / Q000096:biosynthesis*; D008962:Models, Theoretical; D010544:Peroxidases / Q000096:biosynthesis*; D011124:Polyporaceae / Q000378:metabolism
chemical_list: D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase