title: Non-targeted analysis of tea by hydrophilic interaction liquid chromatography and high resolution mass spectrometry.
abstract: Tea is the second most consumed beverage in the world and its consumption has been associated with numerous potential health benefits. Factors such as fermentation methods, geographical origin and season can affect the primary and secondary metabolite composition of tea. In this study, a hydrophilic interaction liquid chromatography (HILIC) method coupled to high resolution mass spectrometry in both positive and negative ionisation modes was developed and optimised. The method when combined with principal component analysis to analyse three different types of tea, successfully distinguished samples into different categories, and provided evidence of the metabolites which differed between them. The accurate mass and high resolution attributes of the mass spectrometric data were utilised and relative quantification data were extracted post-data acquisition on 18 amino acids, showing significant differences in amino acid concentrations between tea types and countries. This study highlights the potential of HILIC chromatography combined with non-targeted mass spectrometric methods to provide a comprehensive understanding of polar metabolites in plant extracts.
authors.affiliation: AgResearch, Private Bag 11008, Tennent Drive, Palmerston North 4410, New Zealand; School of Chemical Science, Auckland University, Auckland 1142, New Zealand.
authors.forename: Karl
authors.initials: K
authors.lastname: Fraser
authors.affiliation: AgResearch, Private Bag 11008, Tennent Drive, Palmerston North 4410, New Zealand; School of Biological Science, Auckland University, Auckland 1142, New Zealand.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Harrison
authors.affiliation: AgResearch, Private Bag 11008, Tennent Drive, Palmerston North 4410, New Zealand.
authors.forename: Geoff A
authors.initials: GA
authors.lastname: Lane
authors.affiliation: AgResearch, Private Bag 11008, Tennent Drive, Palmerston North 4410, New Zealand.
authors.forename: Don E
authors.initials: DE
authors.lastname: Otter
authors.affiliation: School of Chemical Science, Auckland University, Auckland 1142, New Zealand.
authors.forename: Yacine
authors.initials: Y
authors.lastname: Hemar
authors.affiliation: School of Chemical Science, Auckland University, Auckland 1142, New Zealand.
authors.forename: Siew-Young
authors.initials: SY
authors.lastname: Quek
authors.affiliation: AgResearch, Private Bag 11008, Tennent Drive, Palmerston North 4410, New Zealand.
authors.forename: Susanne
authors.initials: S
authors.lastname: Rasmussen
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D013662:Tea / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D013662:Tea