title: Modern mass spectrometry-based structural proteomics.
abstract: Recent developments in the modern mass spectrometry of proteins and peptides have resulted in significant progress in structural proteomics techniques for studying protein structure. A variety of protein structural questions, ranging from defining protein interaction networks to the study of conformational changes and the structure of single proteins, can be addressed using multiple mass spectrometry-based structural proteomics approaches. Each technique provides specific structural information which can be used as experimental structural constraints in protein structure modeling. Here, we describe recent developments in limited proteolysis, surface modification, hydrogen-deuterium exchange, ion mobility, and cross-linking-all combined with modern mass spectrometric techniques-for the studying protein structure.
authors.affiliation: University of Victoria-Genome British Columbia Proteomics Centre, Victoria, British Columbia, Canada.
authors.forename: Evgeniy V
authors.initials: EV
authors.lastname: Petrotchenko
authors.affiliation: University of Victoria-Genome British Columbia Proteomics Centre, Victoria, British Columbia, Canada; Department of Biochemistry and Microbiology, University of Victoria, Petch Building Room 207, Victoria, British Columbia, Canada. Electronic address: christoph@proteincentre.com.
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Borchers
mesh_terms: D041961:Deuterium Exchange Measurement; D013058:Mass Spectrometry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D059748:Proteolysis; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins
keywords: Cross-linking; Hydrogen–deuterium exchange; Limited proteolysis; Mass spectrometry; Prion protein; Structural proteomics; Surface modification