title: Distribution of HLA-A, -B, and -C alleles and HLA/KIR combinations in Han population in China.
abstract: We investigated polymorphisms of the human leukocyte antigen (HLA) class I (A, B, and C) loci of a Han population (n, 239) from the Yunnan province, Southwest China, using high-resolution polymerase chain reaction-Luminex (PCR-Luminex) typing. We combined the HLA data from this study with the KIR genotypes from a previous study of this Han population to analyze the combination of KIR/HLA ligands. A total of 27 HLA-A, 54 HLA-B, and 31 HLA-C alleles were found in this population. The frequencies of A*11:01, A*24:02, B*40:01, B*46:01, C*01:02, C*03:04, and C*07:02 were all > 10%. The following haplotypes were common, with frequencies > 5%: 1 A-B (A*02:07-B*46:01), 2 A-C (A*02:07-C*01:02, and A*11:01-C*07:02), 4 C-B (B*13:01-C*03:04, B*40:01-C*07:02, B*46:01-C*01:02 and B*58:01-C*03:02), and 1 A-C-B (A*02:07-C*01:02-B*46:01). Analysis of KIR3D and their ligands HLA-A3/A11 and HLA-Bw4 showed that the frequencies of 3DL2(+)-A3/A11(+) and 3DL2(+)-A3/A11(-) were 0.527 and 0.473, and the frequencies of 3DL1(+)-Bw4(+), 3DL1(+)-Bw4(-), 3DL1(-)-Bw4(+), and 3DL1(-)-Bw4(-) were 0.552, 0.397, 0.038, and 0.013, respectively. The results of KIR/HLA-C combination analysis showed that all individuals had at least one inhibitory or activating KIR/HLA-C pair, and one KIR/HLA-C pair was the most frequent (157/239), followed by two pairs (46/239), three pairs (33/239), and no pairs (3/239). Comparison of KIR gene and HLA gene and their pair frequency between Yunnan Han and the isolated Han (FYDH) who also lived in Yunnan province showed no significant difference (P > 0.05) in KIR frequencies, but significant differences (P < 0.05) for some HLA allele frequencies. In addition, there was no significant difference (P > 0.05) between the two populations for KIR/HLA pairs.
authors.affiliation: The First People's Hospital in Yunnan Province and The Affiliated Hospital of Kunming Science and Technology University, Kunming 650032, China.
authors.forename: Yunsong
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Kunming 650118, China.
authors.forename: Danfeng
authors.initials: D
authors.lastname: Cao
authors.affiliation: The First People's Hospital in Yunnan Province and The Affiliated Hospital of Kunming Science and Technology University, Kunming 650032, China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Centre for Forensic Science, University of Western Australia, Nedlands, WA 6009, Australia.
authors.forename: J K
authors.initials: JK
authors.lastname: Kulski
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Kunming 650118, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: The First People's Hospital in Yunnan Province and The Affiliated Hospital of Kunming Science and Technology University, Kunming 650032, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Jiang
authors.affiliation: The No. 3 Affiliated Hospital of Kunming Medical University, Kunming 650118, China.
authors.forename: Qianli
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Kunming 650118, China.
authors.forename: Jiankun
authors.initials: J
authors.lastname: Yu
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Kunming 650118, China.
authors.forename: Jingxian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Kunming 650118, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Kunming 650118, China.
authors.forename: Li
authors.initials: L
authors.lastname: Shi
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D015236:HLA-C Antigens / Q000235:genetics; D006239:Haplotypes; D006579:Heterozygote; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008024:Ligands; D015810:Linkage Disequilibrium; D011110:Polymorphism, Genetic; D054340:Receptors, KIR / Q000235:genetics*
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D015395:Histocompatibility Antigens Class I; D008024:Ligands; D054340:Receptors, KIR