title: [Chemical constituents from a Tibetan medicine Meconopsis horridula].
abstract: A phytochemical investigation on the aerial parts of a Tibetan medicine Meconopsis horridula, by solvent extraction, repeated chromatographies on silica gel, Sephadex LH-20, and preparative TLC techniques, led to the isolation of 9 compounds. By spectroscopic analysis and comparison of its 1H and 13C-NMR data with those in literatures, their structures were identified as oleracein E(1), N-( trans-p-coumaroyl) tyramine (2), chrysoeriol (3), apigenin (4), hydnocarpin (5), p-coumaric acid glucosyl ester (6), stigmast-5-ene-3beta-ylformate (7), 3beta-hydroxy-7alpha-ethoxy-24beta-ethylcholest-5-ene (8), and beta-sitosterol (9), respectively, among which compounds 6-8 were isolated from the genus for the first time,and 1,3 were isolated from the species for the first time. A MTT method was applied to evaluate the cytotoxic activity of compounds 14 against the human hepatocellular liver carcinoma cell line (HepG2), and compound 1 showed significant cytotoxicity against HepG2,with its inhibitory rate of 52.2% at 10 micromol x L(-1).
authors.forename: Zhi-Qin
authors.initials: ZQ
authors.lastname: Guo
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Zhu
authors.forename: Shui-Ying
authors.initials: SY
authors.lastname: Zhang
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.forename: Xing-Yun
authors.initials: XY
authors.lastname: Chai
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Tu
mesh_terms: D038861:Medicine, Tibetan Traditional; D015394:Molecular Structure; D028101:Papaveraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D010936:Plant Extracts