title: Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1.
abstract: BACKGROUND
  Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches.
  
  
  RESULTS
  The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant genetic component. Two families with contrasted survival rates were selected. A total of 39 viral genes and five host genes were monitored by real-time PCR. Initial results provided information on (i) the virus cycle of OsHV-1 based on the kinetics of viral DNA replication and transcription and (ii) host defense mechanisms against the virus.
  
  
  CONCLUSIONS
  In the two selected families, the detected amounts of viral DNA and RNA were significantly different. This result suggests that Pacific oysters are genetically diverse in terms of their susceptibility to OsHV-1 infection. This contrasted susceptibility was associated with dissimilar host gene expression profiles. Moreover, the present study showed a positive correlation between viral DNA amounts and the level of expression of selected oyster genes.
authors.forename: Amélie
authors.initials: A
authors.lastname: Segarra
authors.forename: Florian
authors.initials: F
authors.lastname: Mauduit
authors.forename: Nicole
authors.initials: N
authors.lastname: Faury
authors.forename: Suzanne
authors.initials: S
authors.lastname: Trancart
authors.forename: Lionel
authors.initials: L
authors.lastname: Dégremont
authors.forename: Delphine
authors.initials: D
authors.lastname: Tourbiez
authors.forename: Philippe
authors.initials: P
authors.lastname: Haffner
authors.forename: Valérie
authors.initials: V
authors.lastname: Barbosa-Solomieu
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Pépin
authors.forename: Marie-Agnès
authors.initials: MA
authors.lastname: Travers
authors.affiliation: Ifremer (Institut Français de Recherche pour l'Exploitation de la Mer), Unité Santé Génétique et Microbiologie des Mollusques (SG2M), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La Tremblade, France. Tristan.Renault@ifremer.fr.
authors.forename: Tristan
authors.initials: T
authors.lastname: Renault
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D004198:Disease Susceptibility; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics* / Q000378:metabolism; D054884:Host-Pathogen Interactions; D010125:Ostreidae / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D059467:Transcriptome; D019562:Viral Load
chemical_list: D004279:DNA, Viral