title: Quantitative Detection of Purines in Biologically-Relevant Films with TOF-Secondary Ion Mass Spectrometry.
abstract: In purine-depleted environments, thede novopurine biosynthetic pathway is catalyzed to ultimately produce inosine monophosphate (IMP), a purine invisible using current optical microscopy methodology. These enzymes form a complex, termed the "purinosome," to replenish IMP levels. Before cellular chemical imaging may be applied to monitor the distributions and fluctuations in purine levels, it is necessary to develop a scheme to quantitatively detect purines. Here, IMP and other purines in biologically-relevant matrices have been detected quantitatively. These methods provide a TOF-SIMS protocol using C60+primary ions to determine the concentration of biomolecules in a cell such as HeLa at the nanomolar level.
authors.affiliation: Department of Chemistry, Penn State University, University Park, PA 16802.
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Jackson
authors.affiliation: Department of Chemistry, Penn State University, University Park, PA 16802.
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Hue
authors.affiliation: Department of Chemistry, Penn State University, University Park, PA 16802.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Winograd
keywords: C60; TOF-SIMS; Trehalose film; cellular imaging; depth profiling