title: Enhanced degradation of five organophosphorus pesticides in skimmed milk by lactic acid bacteria and its potential relationship with phosphatase production.
abstract: Skimmed milk spiked with five organophosphorus pesticides (OPPs), chlorpyrifos, diazinon, fenitrothion, malathion and methyl parathion, was fermented by ten lactic acid bacteria (LAB) and four strain combinations at 42°C for 24h. OPPs left in the samples at different times were extracted, purified, detected by gas chromatography and calculated for degradation rate constants, based on a first-order reaction model. OPPs degradation was enhanced by the inoculated LAB, resulting in 0.8-225.4% increase in the rate constants. Diazinon and methyl parathion were more stable whereas chlorpyrifos, fenitrothion and malathion were more labile. Lactobacillus brevis 1.0209 showed the strongest acceleration on OPPs degradation while strain combination could bring about a synergy between the strains of lower ability. Phosphatase production of the strains might be one of the key factors responsible for the enhanced OPPs degradation, as the detected phosphatase activities were positively correlated to the measured degradation rate constants of OPPs (r=0.636-0.970, P<0.05).
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR China; Department of Food Science, Northeast Agricultural University, Harbin 150030, PR China; Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR China; Department of Food Science, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Xu
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR China; Department of Food Science, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR China; Department of Food Science, Northeast Agricultural University, Harbin 150030, PR China; Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: zhaoxh@mail.neau.edu.cn.
authors.forename: Xin-Huai
authors.initials: XH
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002417:Cattle; D004390:Chlorpyrifos / Q000737:chemistry / Q000378:metabolism; D002849:Chromatography, Gas; D005278:Fenitrothion / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D019344:Lactic Acid / Q000032:analysis; D052196:Levilactobacillus brevis / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D008294:Malathion / Q000378:metabolism; D008892:Milk / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000378:metabolism*; D010575:Pesticides / Q000737:chemistry / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D009943:Organophosphorus Compounds; D010575:Pesticides; D019344:Lactic Acid; D010744:Phosphoric Monoester Hydrolases; D004390:Chlorpyrifos; D008294:Malathion; D005278:Fenitrothion
keywords: Degradation; Lactic acid bacteria; Organophosphorus pesticides; Phosphatase; Skimmed milk