title: DNA alterations triggered by environmentally relevant polymetallic concentrations in marine clams Ruditapes philippinarum and polychaete worms Hediste diversicolor.
abstract: We exposed marine clams (Ruditapes philippinarum) and aquatic worms (Hediste diversicolor) to environmentally relevant concentrations of two metal mixtures each containing three divalent metals [(C₁ in µg/L) cadmium (Cd) 1, mercury (Hg) 0.1, and lead (Pb) 4] and [C₂ in µg/L) Cd 17, Hg 1.1, and Pb 55]. Animals collected in the Arcachon Bay were exposed for 8 days in microcosms made up of a mixed biotope consisting of a water column and natural marine sediment both taken up from the Arcachon Bay. Bioaccumulation analysis showed a significant increase of Cd, Hg, and Pb in clams, particularly at C₂ concentration in the water column reaching, in soft body, 2.3 ± 0.3 µg Cd/g, 0.7 ± 0.2 µg Hg/g, and 45 µg Pb/g dry weight (dw). DNA alterations and upregulation of the cox1 mitochondrial gene were also observed in clam gill after exposure to the metal blend. For worms exposed to the C₂ metal blend, DNA alterations and significant increase of Cd and Hg concentrations were observed reaching 0.5 ± 0.1 µg Cd/g and 2 ± 0.6 µg Hg/g dw.
authors.affiliation: CNRS, UMR EPOC 5805, University of Bordeaux, Arcachon Marine Station, Place du Dr Peyneau, 33120, Arcachon, France, a.dedeh@epoc.u-bordeaux1.fr.
authors.forename: Amina
authors.initials: A
authors.lastname: Dedeh
authors.forename: Aurélie
authors.initials: A
authors.lastname: Ciutat
authors.forename: Damien
authors.initials: D
authors.lastname: Tran
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Bourdineaud
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000235:genetics*; D004249:DNA Damage; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000737:chemistry; D019216:Metals, Heavy / Q000378:metabolism / Q000633:toxicity*; D011077:Polychaeta / Q000235:genetics*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical