title: Optimization of laccase production by two strains of Ganoderma lucidum using phenolic and metallic inducers.
abstract: Ganoderma lucidum (Curtis) P. Karst is a white rot fungus that is able to degrade the lignin component in wood. The ability of two strains of this species to produce the ligninolytic enzyme laccase was assessed. After the evaluation of induction with heavy metals and phenolic compounds, it was found that among the tested substances, copper and ferulic acid are the best laccase inducers. It was also observed that the two types of inducers (phenolic and metallic) produce different electrophoretic patterns of laccase activity. Optimized concentrations of inducers were obtained through a factorial design and the thermal stability of optimized supernatants was studied at a wide range of acidic pH. We found that the enzyme is more thermostable at higher pH values.
authors.affiliation: Centro de Investigación y Extensión Forestal Andino Patagónico (CIEFAP), Esquel, Chubut, Argentina. Electronic address: fkuhar@ciefap.org.ar.
authors.forename: Francisco
authors.initials: F
authors.lastname: Kuhar
authors.affiliation: Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Leandro
authors.initials: L
authors.lastname: Papinutti
mesh_terms: D003300:Copper / Q000494:pharmacology; D003373:Coumaric Acids; D042845:Laccase / Q000096:biosynthesis*; D008345:Manganese / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology; D020940:Reishi / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D003373:Coumaric Acids; D010636:Phenols; D008345:Manganese; D003300:Copper; C004999:ferulic acid; D042845:Laccase
keywords: Cobre; Compuestos fenólicos; Copper; Ganoderma; Inducción; Induction; Lacasa; Laccase; Optimización; Optimization; Phenolic compounds