title: Cloning, overexpression, purification and preliminary X-ray analysis of the protein kinase domain of enhanced disease resistance 1 (EDR1) from Arabidopsis thaliana.
abstract: Enhanced disease resistance 1 is a member of the Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK) family that negatively regulates disease resistance, ethylene-induced senescence and programmed cell death in response to both abiotic and biotic stresses. A catalytically inactive form of the EDR1 kinase domain was successfully cloned, expressed, purified and crystallized. Crystallization was conducted in the presence of the ATP analogue AMP-PNP. The crystals belonged to space group P3221 and contained two molecules in the asymmetric unit. The crystals diffracted X-rays to 2.55 Å resolution.
authors.affiliation: EMBL Hamburg Outstation, Germany.
authors.forename: Heidi
authors.initials: H
authors.lastname: Kaljunen
authors.affiliation: Photon Science, DESY, Germany.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Panneerselvam
authors.affiliation: University of Hamburg, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Mueller-Dieckmann
mesh_terms: D000266:Adenylyl Imidodiphosphate / Q000737:chemistry*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry* / Q000276:immunology; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C423568:EDR1 protein, Arabidopsis; D011994:Recombinant Proteins; D000266:Adenylyl Imidodiphosphate
keywords: Arabidopsis thaliana; MAPKKK family; enhanced disease resistance 1