title: Effect of maternal protein restriction on liver metabolism in rat offspring.
abstract: Consequences of gestational protein restriction (GPR) on liver metabolism in rat offspring were investigated. Pregnant dams were divided into groups: normal (NP, 17% casein) or low-protein diet (LP, 6% casein). Livers were collected from 30-day-old offspring (d30) for analysis or isolation of mitochondria. At d30, hepatic and muscle glycogen was increased in LP group. Mitochondrial swelling and oxygen uptake (recorded with a Clark-type electrode) were significantly reduced in NP female and LP pups. Thiobarbituric acid reactive substances production was lower in females (NP or LP), suggesting significant inhibition of lipid peroxidation. Measurement of mitochondrial respiration (states 3 and 4 stimulated by succinate) showed a higher ADP/O ratio in LP pups, particularly females, suggesting higher phosphorylation efficiency. In the 1st month of life, under our experimental conditions, GPR protects liver mitochondria against oxidative stress and females seem to be more resistant or more suitable for survival.
authors.affiliation: Biomedical Sciences Graduate Program, Centro Universitário Hermínio Ometto, Uniararas, Av Maximiliano Baruto 500, 13607-339, Araras, SP, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Moraes
authors.forename: Hércules J
authors.initials: HJ
authors.lastname: Rebelato
authors.forename: Maria Esmeria C
authors.initials: ME
authors.lastname: Amaral
authors.forename: Thais Marangoni
authors.initials: TM
authors.lastname: Resende
authors.forename: Eduarda V C
authors.initials: EV
authors.lastname: Silva
authors.forename: Marcelo A M
authors.initials: MA
authors.lastname: Esquisatto
authors.forename: Rosana
authors.initials: R
authors.lastname: Catisti
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism* / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D019069:Cell Respiration / Q000502:physiology; D018753:Diet, Protein-Restricted / Q000379:methods; D005260:Female; D006003:Glycogen / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D008099:Liver / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology; D010100:Oxygen / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism*; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D006003:Glycogen; D010100:Oxygen