title: Local rhBMP-2 injection after drill-hole injury in rats: does it have systemic effects?
abstract: The aim of this study is to investigate the histopathological findings of drill hole healing and interactions of parathyroid hormone (PTH), &#946;-catenin and transcription factor-4 (TCF7L2/Tcf-4) after local application of recombinant human bone morphogenic protein-2 (rhBMP-2). Sprague Dawley rats were used in two groups of femoral cortex hole model. In the non-treated group, a hole was opened with a 3 mm K-wire in the distal and mid third junction of the right femur. In the treated group, local rhBMP-2 protein was injected into the similar femoral hole. Sterile 18M H2O was injected into the femoral hole at contralateral femur. There was more subperiosteal membranous bone reaction in the group treated with rhBMP-2 injection compared to the non-treated group. This was also proven immunohistochemically in both ipsilateral and contralateral femur with increased anti bone morphogenic protein-2 (anti BMP-2) expression. Moreover, there was an increased subperiosteal reaction at the contralateral femur. Also, in the treated group, PTH expression was increased in cells that form callus, and nuclear beta-catenin expression was increased in chondrocytes of periosteal ossification. Future studies should try to find whether the effects of rhBMP-2 on PTH and Wnt signaling pathway changes with different fracture models, also the systemic effects of local rhBMP-2 application should be investigated.
authors.affiliation: Department of Pathology, Medical School of Pamukkale University, Denizli, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Demirkan
authors.affiliation: Department of Orthopaedics and Traumatology, Medical School of Pamukkale University Denizli, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Demirkan
authors.affiliation: Department of Pathology, Ankara University Medical School, Ankara, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Aksoy
authors.affiliation: Department of Orthopaedics and Traumatology, Ozel Mardin Park Hastanesi, Mardin, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Ozdemir
authors.affiliation: Department of Orthopaedics and Traumatology, Medical School of Pamukkale University Denizli, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Akman
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D016723:Bone Remodeling / Q000187:drug effects*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D005264:Femoral Fractures / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D005269:Femur / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D017102:Fracture Healing / Q000187:drug effects*; D007267:Injections; D008297:Male; D010281:Parathyroid Hormone / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; C495268:Ctnnb1 protein, rat; D004268:DNA-Binding Proteins; D010281:Parathyroid Hormone; D011994:Recombinant Proteins; C491192:Tcf4 protein, rat; D000073940:Transcription Factor 4; D014157:Transcription Factors; D051176:beta Catenin