title: Pattern recognition of estradiol, testosterone and dihydrotestosterone in children's saliva samples using stochastic microsensors.
abstract: Stochastic microsensors based on diamond paste and three types of electroactive materials (maltodextrin (MD), α-cyclodextrin (α-CD) and 5,10,15,20-tetraphenyl-21H,23H porphyrin (P)) were developed for the assay of estradiol (E2), testosterone (T2) and dihydrotestosterone (DHT) in children's saliva. The main advantage of utilization of such tools is the possibility to identify and quantify all three hormones within minutes in small volumes of childen's saliva. The limits of quantification obtained for DHT, T2, and E2 (1 fmol/L for DHT, 1 pmol/L for T2, and 66 fmol/L for E2) determined using the proposed tools allows the utilization of these new methods with high reliability for the screening of saliva samples from children. This new method proposed for the assay of the three hormones overcomes the limitations (regarding limits of determination) of ELISA method which is the standard method used in clinical laboratories for the assay of DHT, T2, and E2 in saliva samples. The main feature of its utilization for children's saliva is to identify earlier problems related to early puberty and obesity.
authors.affiliation: 1] Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania [2] Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, Bucharest, Romania.
authors.forename: Raluca-Ioana
authors.initials: RI
authors.lastname: Stefan-van  Staden
authors.affiliation: 1] Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania [2] Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, Bucharest, Romania.
authors.forename: Livia Alexandra
authors.initials: LA
authors.lastname: Gugoaşă
authors.affiliation: Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Calenic
authors.affiliation: Institute for Environmental Studies, VU University Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
authors.forename: Juliette
authors.initials: J
authors.lastname: Legler
mesh_terms: D015374:Biosensing Techniques; D002648:Child; D002675:Child, Preschool; D013196:Dihydrotestosterone / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008297:Male; D010363:Pattern Recognition, Automated; D012463:Saliva / Q000378:metabolism*; D013269:Stochastic Processes; D013739:Testosterone / Q000378:metabolism*
chemical_list: D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol