title: A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications.
abstract: A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG™, is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.
authors.affiliation: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden. karim@organ.su.se jeb@organ.su.se.
authors.forename: K
authors.initials: K
authors.lastname: Engelmark Cassimjee
authors.forename: M
authors.initials: M
authors.lastname: Kadow
authors.forename: Y
authors.initials: Y
authors.lastname: Wikmark
authors.forename: M
authors.initials: M
authors.lastname: Svedendahl Humble
authors.forename: M L
authors.initials: ML
authors.lastname: Rothstein
authors.forename: D M
authors.initials: DM
authors.lastname: Rothstein
authors.forename: J-E
authors.initials: JE
authors.lastname: Bäckvall
mesh_terms: D055162:Biocatalysis; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D005898:Glass; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D010105:Oxygenases / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D002787:Sterol Esterase / Q000737:chemistry / Q000378:metabolism; D000637:Transaminases / Q000737:chemistry / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011108:Polymers; D010105:Oxygenases; C047193:2,5-diketocamphane 1,2-monooxygenase; D000637:Transaminases; D002787:Sterol Esterase; D008049:Lipase; C431349:lipase B, Candida antarctica