title: Simultaneous determination of 11 bioactive compounds in Jaeumganghwa-tang by high performance liquid chromatography-diode array detection.
abstract: BACKGROUND
  Jaeumganghwa-tang (JEGH) is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus.
  
  
  OBJECTIVE
  A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for simultaneous determination of 11 major compounds such as 5- hydroxymethylfurfural, mangiferin, paeoniflorin, nodakenin, naringin, hesperidin, decursinol, berberine, glycyrrhizin, atractylenolide III and decursin, in JEGH.
  
  
  METHODS
  The separation was conducted on Shishedo C18 column with gradient elution of 0.1% trifluoroacetic acid-acetonitrile. Detection of wavelength was set at 205, 250, 280 and 330 nm.
  
  
  RESULTS
  The developed analysis showed a good linearity (R (2) >0.9997). The range of limit of detection and limit of quantification were observed from 0.04 to 0.43 and from 0.11 to 1.30, respectively. The intra- and inter-day test relative standard deviations (RSD) were less than 3% and the accuracy was 95.98-108.44%. The recoveries were between 92.75% and 109.19% and RSD range of recoveries was measured from 0.52% to 2.78%.
  
  
  CONCLUSIONS
  This HPLC-DAD method can be successfully applied for simultaneous determination of 11 major compounds in JEGH samples.
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Yun
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Jiwoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Min Rye
authors.initials: MR
authors.lastname: Eom
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea ; Research Institute of Biotechnology, Kangwon National University, Chuncheon, Republic of Korea.
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
keywords: High performance liquid chromatography-diode array detection; Jaeumganghwa-tang; liquid chromatography-mass spectrometry; simultaneous determination