title: miR-143 inhibits NSCLC cell growth and metastasis by targeting Limk1.
abstract: MicroRNAs (miRNAs) have essential roles in carcinogenesis and tumor progression. Here, we investigated the roles and mechanisms of miR-143 in non-small cell lung cancer (NSCLC). miR-143 was significantly decreased in NSCLC tissues and cell lines. Overexpression of miR-143 suppressed NSCLC cell proliferation, induced apoptosis, and inhibited migration and invasion in vitro. Integrated analysis identified LIM domain kinase 1 (Limk1) as a direct and functional target of miR-143. Overexpression of Limk1 attenuated the tumor suppressive effects of miR-143 in NSCLC cells. Moreover, miR-143 was inversely correlated with Limk1 expression in NSCLC tissues. Together, our results highlight the significance of miR-143 and Limk1 in the development and progression of NSCLC.
authors.affiliation: Department of Thoracic-Cardio Surgery, the First Affiliated Hospital of PLA General Hospital, Beijing 100048, China. hui_xiaa@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.affiliation: Department of Medical Oncology, the General Hospital of People's Liberation Army, Beijing 100853, China. shengjie_sunn@126.com.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Thoracic Surgery, the General Hospital of People's Liberation Army, Beijing 100853, China. wang_boa@126.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, the General Hospital of People's Liberation Army, Beijing 100853, China. wang_taow@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, the General Hospital of People's Liberation Army, Beijing 100853, China. liang_cyc@126.com.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Liang
authors.affiliation: Department of Thoracic Surgery, the General Hospital of People's Liberation Army, Beijing 100853, China. li_gguo@126.com.
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Cancer Prevention and Therapy of Tianjin, Department of Senior Ward, Tianjin Medical University Cancer Institute and Hospital, Huanhu Xilu, Hexi District, Tianjin 300060, China. huang_chongbiao@126.com.
authors.forename: Chongbiao
authors.initials: C
authors.lastname: Huang
authors.affiliation: Key Laboratory of Cancer Prevention and Therapy of Tianjin, Department of Senior Ward, Tianjin Medical University Cancer Institute and Hospital, Huanhu Xilu, Hexi District, Tianjin 300060, China. dalingqi1999@126.com.
authors.forename: Daliang
authors.initials: D
authors.lastname: Qi
authors.affiliation: Department of Thoracic Surgery, the General Hospital of People's Liberation Army, Beijing 100853, China. chuxiangyang52301@126.com.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Chu
mesh_terms: D017209:Apoptosis; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D054461:Lim Kinases / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*
chemical_list: C542359:MIRN143 microRNA, human; D035683:MicroRNAs; C515892:LIMK1 protein, human; D054461:Lim Kinases