title: Recruitment of Gr1(+)CD11b (+)F4/80 (+) population in the bone marrow and spleen by irradiation-induced pulmonary damage.
abstract: Radiation-induced lung injury is a kind of sterile inflammation, which may lead to morbidity and mortality. The mechanism by which ionizing radiation activate the immune system is not well understood. In the present study, we have investigated the immunological responses induced by local irradiation-induced damage in mouse lung. The left lungs of C57BL/6 mice were irradiated at a high dose of 100 Gy. The histology of the lungs and spleen showed evidences of alveolar inflammation and congestion at 2 weeks after X-ray treatment. Also, prominent increase in cells expressing the cell surface markers, Gr(+)CD11b(+)F4/80(+) and Ly6C(+) Ly6G(+) were observed 2 weeks after X-ray treatment (100 Gy). Gr1(+)CD11b(+)F4/80(+) cell depletion by clodronate treatment reversed the histological effects and also failed to recruit Gr(+)CD11b(+) cells or F4/80(+) cells caused by irradiation. The origin of recruited Gr1(+)CD11b(+) cells was found to be a mixed resident and recruited phenotype.
authors.affiliation: Department of Immunology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.
authors.forename: Suganya
authors.initials: S
authors.lastname: Thanasegaran
authors.forename: Sachiko
authors.initials: S
authors.lastname: Ito
authors.forename: Naomi
authors.initials: N
authors.lastname: Nishio
authors.forename: Mohammad Nizam
authors.initials: MN
authors.lastname: Uddin
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Isobe
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism*; D000950:Antigens, Ly / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism; D016026:Bone Marrow Transplantation; D039481:CD11b Antigen / Q000378:metabolism*; D002452:Cell Count; D004002:Clodronic Acid / Q000494:pharmacology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D008212:Lymphocyte Depletion; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011650:Pulmonary Alveoli / Q000276:immunology / Q000473:pathology / Q000528:radiation effects; D012333:RNA, Messenger / Q000096:biosynthesis; D017564:Radiation Pneumonitis / Q000276:immunology* / Q000473:pathology; D013154:Spleen / Q000187:drug effects* / Q000276:immunology / Q000473:pathology
chemical_list: D000943:Antigens, Differentiation; D000950:Antigens, Ly; D039481:CD11b Antigen; D016207:Cytokines; C494192:Ly-6C antigen, mouse; C480309:Ly6G antigen, mouse; D012333:RNA, Messenger; C036763:monocyte-macrophage differentiation antigen; D004002:Clodronic Acid