title: Early stages of we/we wal/wal mouse hair morphogenesis: light and fluorescent microscopy of the whole-mount epidermis.
abstract: In adult skin, hair follicles cyclically self-renew in a manner that recapitulates embryonic hair follicle morphogenesis. The most common pathology of hair in adults is alopecia, which is hair loss to different extent. There are a number of murine models of alopecia including spontaneous mutations. In the present study, we worked with double homozygous we/we wal/wal mice which demonstrate symptoms closely resembling human alopecia. Using whole-mount preparations of epidermis of E18.5 embryos we show that hair follicle defects can be revealed as early as during embryonic morphogenesis in these mutants. The number of hair follicles was reduced almost 1.5-fold in mutant skin. The shape of the early stage small follicles was altered in mutant animals as compared to control ones. Additionally, follicles of mutant embryos were wider at the point of conjunction with interfollicular epidermis. We believe that the mutant mice studied represent a fascinating model to address the problem of hair loss. We demonstrated alterations in the morphogenesis of embryonic hair follicle in we/we wal/wal double homozygous mice developing alopecia postnatally. We suppose that incorrect morphogenesis of hair follicles during embryogenesis is closely related to alopecia in the adult life. Unveiling the mechanisms involved in altered embryogenesis may elucidate the pathogenesis of alopecia.
authors.affiliation: Department of Biomedical Technologies, Pirogov Russian National Research Medical University, 1 Ostrovityanova, Moscow 117997, Russia.
authors.forename: Alexandra
authors.identifier: 0000-0001-7300-8466
authors.initials: A
authors.lastname: Rippa
authors.affiliation: Laboratory of Cell Basics for Cancer Research, Engelhardt Institute of Molecular Biology, 32 Vavilov Street, Moscow 119991, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Leonova
authors.affiliation: Laboratory of Cell Basics for Cancer Research, Engelhardt Institute of Molecular Biology, 32 Vavilov Street, Moscow 119991, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Popenko
authors.affiliation: Laboratory of Cell Proliferation, N.K. Koltsov Institute of Developmental Biology, 26 Vavilov Street, Moscow 119334, Russia.
authors.forename: Andrey
authors.initials: A
authors.lastname: Vasiliev
authors.affiliation: Laboratory of Cell Proliferation, N.K. Koltsov Institute of Developmental Biology, 26 Vavilov Street, Moscow 119334, Russia.
authors.forename: Vasily
authors.identifier: 0000-0001-5006-4254
authors.initials: V
authors.lastname: Terskikh
authors.affiliation: Laboratory of Cell Proliferation, N.K. Koltsov Institute of Developmental Biology, 26 Vavilov Street, Moscow 119334, Russia ; Department of Cell Biology and Histology, Lomonosov Moscow State University, 1 Leninskiye Gory, Moscow 119234, Russia.
authors.forename: Ekaterina
authors.identifier: 0000-0001-5405-0212
authors.initials: E
authors.lastname: Vorotelyak
mesh_terms: D000505:Alopecia / Q000235:genetics* / Q000473:pathology; D000818:Animals; D047108:Embryonic Development / Q000235:genetics; D004817:Epidermis / Q000254:growth & development / Q000648:ultrastructure*; D018859:Hair Follicle / Q000254:growth & development* / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D009024:Morphogenesis / Q000235:genetics*; D009154:Mutation