title: Evaluation of plant phenolic metabolites as a source of Alzheimer's drug leads.
abstract: Epidemiological studies have proven an association between consumption of polyphenols and prevention of Alzheimer's disease, the most common form of dementia characterized by extracellular deposition of amyloid beta plaques. The aim of this study is pharmacological screening of the aqueous alcohol extract of Markhamia platycalyx leaves, Schotia brachypetala leaves and stalks, and piceatannol compared to aqueous alcohol extract of Camellia sinensis leaves as potential Alzheimer's disease drugs. LC-HRESI(-ve)-MS(n) was performed to identify phenolics' profile of Schotia brachypetala stalks aqueous alcohol extract and revealed ten phenolic compounds as first report: daidzein, naringin, procyanidin isomers, procyanidin dimer gallate, quercetin 3-O-rhamnoside, quercetin 3-O-glucuronide, quercetin hexose gallic acid, quercetin hexose protocatechuic acid, and ellagic acid. Alzheimer's disease was induced by a single intraperitoneal injection of LPS. Adult male Swiss albino mice were divided into groups of 8-10 mice each receiving treatment for six days. In vivo behavioral tests (Y maze and object recognition) and in vitro estimation of amyloid beta 42 by ELISA showed significant differences between results of treated and nontreated animals.
authors.affiliation: Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, Egypt.
authors.forename: Yara
authors.initials: Y
authors.lastname: Hassaan
authors.affiliation: Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, Egypt.
authors.forename: Heba
authors.initials: H
authors.lastname: Handoussa
authors.affiliation: Laboratory of Analytical and Environmental Chemistry, Department of Chemistry, Humboldt-Universität zu Berlin, 12489 Berlin, Germany.
authors.forename: Ahmed H
authors.identifier: 0000-0002-2012-5893
authors.initials: AH
authors.lastname: El-Khatib
authors.affiliation: Laboratory of Analytical and Environmental Chemistry, Department of Chemistry, Humboldt-Universität zu Berlin, 12489 Berlin, Germany.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Linscheid
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt ; Department of Pharmacology and Toxicology, Faculty of Pharmacy & Biotechnology, German University in Cairo, Cairo 11835, Egypt.
authors.forename: Nesrine
authors.identifier: 0000-0003-3124-5118
authors.initials: N
authors.lastname: El Sayed
authors.affiliation: Department of Pharmaceutical Biology, Faculty of Pharmacy, Ain-Shams University, Cairo 11613, Egypt ; Department of Pharmaceutical Biology, Faculty of Pharmacy, British University in Cairo, Cairo 11613, Egypt.
authors.forename: Nahla
authors.initials: N
authors.lastname: Ayoub
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry; D005419:Flavonoids / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols