title: Same-day subtyping of Campylobacter jejuni and C. coli isolates by use of multiplex ligation-dependent probe amplification-binary typing.
abstract: Campylobacteriosis is the most commonly reported form of human bacterial gastroenteritis in the world. Sound identification of infectious sources requires subtyping, but the most widely used methods have turnaround times measured in days and require specialist equipment and skills. A multiplex ligation-dependent probe amplification-binary typing (MBiT) assay was developed for subtyping Campylobacter jejuni and Campylobacter coli. It was tested on 245 isolates, including recent isolates from Belgium and New Zealand, and compared to multilocus sequence typing (MLST). When used in an outbreak setting, MBiT identified the predominant genotype and possible additional cases days before pulsed-field gel electrophoresis (PFGE) results were available. MBiT was more discriminatory than MLST and, being a single assay with results produced within 6 h, was more rapid and cost-effective than both MLST and PFGE. In addition, MBiT requires only basic molecular biology equipment and skills.
authors.affiliation: Institute of Environmental Science and Research (ESR), Christchurch, New Zealand angela.cornelius@esr.cri.nz.
authors.forename: Angela J
authors.initials: AJ
authors.lastname: Cornelius
authors.affiliation: National Reference Center for Campylobacter, Saint Pierre University Hospital, Brussels, Belgium Ecole de Santé Publique, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Olivier
authors.initials: O
authors.lastname: Vandenberg
authors.affiliation: Institute of Environmental Science and Research (ESR), Christchurch, New Zealand.
authors.forename: Beth
authors.initials: B
authors.lastname: Robson
authors.affiliation: Institute of Environmental Science and Research (ESR), Christchurch, New Zealand.
authors.forename: Brent J
authors.initials: BJ
authors.lastname: Gilpin
authors.affiliation: Institute of Environmental Science and Research (ESR), Christchurch, New Zealand.
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Brandt
authors.affiliation: Institute of Environmental Science and Research (ESR), Christchurch, New Zealand.
authors.forename: Paula
authors.initials: P
authors.lastname: Scholes
authors.affiliation: National Reference Center for Campylobacter, Saint Pierre University Hospital, Brussels, Belgium.
authors.forename: Delphine
authors.initials: D
authors.lastname: Martiny
authors.affiliation: Institute of Environmental Science and Research (ESR), Wellington, New Zealand.
authors.forename: Philip E
authors.initials: PE
authors.lastname: Carter
authors.affiliation: MRC-Holland, Amsterdam, The Netherlands.
authors.forename: Paul
authors.initials: P
authors.lastname: van Vught
authors.affiliation: MRC-Holland, Amsterdam, The Netherlands.
authors.forename: Jan
authors.initials: J
authors.lastname: Schouten
authors.affiliation: Institute of Environmental Science and Research (ESR), Christchurch, New Zealand.
authors.forename: Stephen L W
authors.initials: SL
authors.lastname: On
mesh_terms: D001530:Belgium; D002169:Campylobacter Infections / Q000382:microbiology*; D017000:Campylobacter coli / Q000145:classification* / Q000235:genetics; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009520:New Zealand; D012680:Sensitivity and Specificity; D013997:Time Factors