title: Body weight management effect of burdock (Arctium lappa L.) root is associated with the activation of AMP-activated protein kinase in human HepG2 cells.
abstract: Burdock (Arcticum lappa L.) root is used in folk medicine and also as a vegetable in Asian countries. In the present study, burdock root treatment significantly reduced body weight in rats. To evaluate the bioactive compounds, we successively extracted the burdock root with ethanol (AL-1), and fractionated it with n-hexane (AL-2), ethyl acetate (AL-3), n-butanol (AL-4), and water (AL-5). Among these fractions, AL-2 contained components with the most effective hypolipidemic potential in human hepatoma HepG2 cells. AL-2 decreased the expression of fatty acid synthase (FASN) and inhibited the activity of acetyl-coenzyme A carboxylase (ACC) by stimulating AMP-activated protein kinase (AMPK) through the LKB1 pathway. Three active compounds were identified from the AL-2, namely α-linolenic acid, methyl α-linolenate, and methyl oleate. These results suggest that burdock root is expected to be useful for body weight management.
authors.affiliation: Department of Pharmacy, Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung, Taiwan, ROC.
authors.forename: Daih-Huang
authors.initials: DH
authors.lastname: Kuo
authors.affiliation: Department of Pharmacy, Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung, Taiwan, ROC.
authors.forename: Ming-Chi
authors.initials: MC
authors.lastname: Hung
authors.affiliation: Department of General Surgery, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan, ROC.
authors.forename: Chao-Ming
authors.initials: CM
authors.lastname: Hung
authors.affiliation: Graduate Institute of Pharmaceutical Chemistry, College of Pharmacy, China Medical University, Taichung, Taiwan, ROC.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung, Taiwan, ROC.
authors.forename: Fu-An
authors.initials: FA
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung, Taiwan, ROC.
authors.forename: Po-Chuen
authors.initials: PC
authors.lastname: Shieh
authors.affiliation: Department of Food Science, Rutgers University, New Brunswick, NJ, USA.
authors.forename: Chi-Tang
authors.initials: CT
authors.lastname: Ho
authors.affiliation: Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, Taiwan, ROC; Institute of Biochemistry, College of Life Science, National Chung Hsing University, Taichung, Taiwan, ROC.
authors.forename: Tzong-Der
authors.initials: TD
authors.lastname: Way
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D031170:Arctium / Q000737:chemistry*; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010936:Plant Extracts; D055372:AMP-Activated Protein Kinases