title: Hemocompatibility and selective cell fate of polydopamine-assisted heparinized PEO/PLLA composite coating on biodegradable AZ31 alloy.
abstract: Biodegradable magnesium and its alloys have attracted much attention, as they have been used as cardiovascular stents recently because of their biodegradation after implantation. However, their corrosion resistance, hemocompatibility and surface biocompatibility are needed for practical applications. In this work, heparinization of the plasma electrolytic oxidation/poly(l-lactic acid) (PEO/PLLA) composite coating on biodegradable AZ31 alloy was achieved by the strong adhesion of mussel-inspired polydopamine (PDAM). The corrosion resistance of the coated substrates was evaluated in simulated body fluid. In particular, the hemolysis ratio and platelet adhesion tests were conducted to evaluate the hemocompatibility of the composite coatings. The in vitro cytotoxicity of the composite coatings was evaluated with human umbilical vein endothelial cells (HUVECs). The adhesion and proliferation of HUVECs and human umbilical artery smooth muscle cells (HUASMCs) directly incubated on the composite coatings were also investigated. The results showed that although PDAM modification and further heparinization reduced the corrosion resistance of the PEO/PLLA composite coating, the protection of the coating for the substrate was mainly maintained. Moreover, PDAM modification and further heparinization significantly suppressed the adhesion of platelets and had little influence on sustaining a low hemolysis ratio thus resulting in good surface hemocompatibility of the composite coating. The in vitro cell test demonstrated that none of the composite coatings presented obvious cytotoxicity. Significantly, after surface heparinization, the composite coating became more suitable for HUVEC growth and simultaneously inhibited HUASMC growth. The results show that further modification of the PEO/PLLA composite coating on biodegradable magnesium alloy is a promising method to obtain good surface hemocompatibility for anticoagulation and to regulate the cell fate for fast re-endothelialization while sustaining the corrosion resistance of biodegradable magnesium-based cardiovascular stents.
authors.affiliation: Institute of Materials, Shanghai University, Shanghai 200072, P.R. China. Electronic address: rocky.wei@163.com.
authors.forename: Zhongling
authors.initials: Z
authors.lastname: Wei
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Tian
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, P.R. China. Electronic address: xyliu@mail.sic.ac.cn.
authors.forename: Xuanyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Materials, Shanghai University, Shanghai 200072, P.R. China.
authors.forename: Bangxin
authors.initials: B
authors.lastname: Zhou
mesh_terms: D000497:Alloys / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003343:Corrosion; D004572:Electrolysis; D006461:Hemolysis / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing / Q000379:methods*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000737:chemistry / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D014469:Umbilical Arteries / Q000166:cytology
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D007211:Indoles; C586533:Mg-Al-Zn-Mn-Si-Cu alloy; D058626:Plasma Gases; D011091:Polyesters; D011108:Polymers; C568283:polydopamine; D019344:Lactic Acid; C033616:poly(lactide); D006493:Heparin
keywords: Cytocompatibility; Hemocompatibility; Heparinization; Magnesium alloy; PEO/PLLA composite coating