title: Isolation and selection of new biosurfactant producing bacteria from degraded palm kernel cake under liquid state fermentation.
abstract: Biosurfactants are surface-active compounds produced by different microorganisms. The aim of this study was to introduce palm kernel cake (PKC) as a novel substrate for biosurfactant production using a potent bacterial strain under liquid state fermentation. This study was primarily based on the isolation and identification of biosurfactant-producing bacteria that could utilize palm kernel cake as a new major substrate. Potential bacterial strains were isolated from degraded PKC and screened for biosurfactant production with the help of the drop collapse assay and by analyzing the surface tension activity. From the screened isolates, a new strain, SM03, showed the best and most consistent results, and was therefore selected as the most potent biosurfactant-producing bacterial strain. The new strain was identified as Providencia alcalifaciens SM03 using the Gen III MicroPlate Biolog Microbial Identification System. The yield of the produced biosurfactant was 8.3 g/L.
authors.affiliation: Bioenvironmental Engineering Research Centre (BERC), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia.
authors.forename: Parveen
authors.initials: P
authors.lastname: Jamal
authors.forename: Shajrat
authors.initials: S
authors.lastname: Mir
authors.forename: Md Zahangir
authors.initials: MZ
authors.lastname: Alam
authors.forename: Wan M Fazli
authors.initials: WM
authors.lastname: Wan Nawawi
mesh_terms: D028023:Arecaceae / Q000382:microbiology; D001431:Bacteriological Techniques; D005285:Fermentation; D000073878:Palm Oil; D010938:Plant Oils; D011532:Providencia / Q000302:isolation & purification* / Q000378:metabolism*; D012037:Refuse Disposal; D012639:Seeds / Q000382:microbiology; D013501:Surface-Active Agents / Q000302:isolation & purification / Q000378:metabolism*; D018505:Waste Management / Q000379:methods
chemical_list: D010938:Plant Oils; D013501:Surface-Active Agents; D000073878:Palm Oil