title: Antepartum screening for group B Streptococcus by three FDA-cleared molecular tests and effect of shortened enrichment culture on molecular detection rates.
abstract: Neonatal Streptococcus agalactiae infections cause significant morbidity and mortality, and antenatal screening is recommended. We compared three U.S. Food and Drug Administration (FDA)-cleared nucleic acid amplification tests (NAATs) to culture using 314 vaginal/rectal swabs after 18 to 24 h (recommended period) and 4 to 8 h (shortened period) of broth enrichment. Agreement of the NAATs with each other was high (97.1% to 98.4%), but culture was less sensitive than all NAATs (67% to 73%). A shortened period of broth culture enrichment resulted in 1 false-negative result in 68 (1.5%). The NAATs performed comparably and were more sensitive than culture.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
authors.forename: Brianne A
authors.initials: BA
authors.lastname: Couturier
authors.affiliation: ARUP Laboratories, Salt Lake City, Utah, USA.
authors.forename: Trent
authors.initials: T
authors.lastname: Weight
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
authors.forename: Haley
authors.initials: H
authors.lastname: Elmer
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA robert.schlaberg@path.utah.edu.
authors.forename: Robert
authors.initials: R
authors.lastname: Schlaberg
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D003951:Diagnostic Errors; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008403:Mass Screening / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000235:genetics*; D011296:Prenatal Diagnosis / Q000379:methods*; D012007:Rectum / Q000382:microbiology; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000302:isolation & purification*; D013997:Time Factors; D014481:United States; D014621:Vagina / Q000382:microbiology