title: MicroRNA-32 inhibits osteosarcoma cell proliferation and invasion by targeting Sox9.
abstract: Increasing reports suggest that discovery of microRNAs (miRNAs) might provide a novel therapeutical target for human cancers, including osteosarcoma. Previous studies have shown that miR-32 was dysregulated in breast and endometrial cancer. However, its biological roles in osteosarcoma remain unclear. In the current study, we found that miR-32 was significantly down-regulated in osteosarcoma tissues, compared with the adjacent normal tissues. In vitro studies further demonstrated that miR-32 mimics were able to suppress, while its antisense oligos promoted cell proliferation in Saos-2 and U2OS cells. At the molecular level, our data further revealed that expression of Sox9 was negatively regulated by miR-32. Therefore, our results identify an important role for miR-32 in the osteosarcoma through regulating Sox9 expression.
authors.affiliation: Department of Orthopaedics, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, No.197, Ruijin 2nd Road, Shanghai, 200025, China.
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Xu
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Wan
authors.forename: Yao-Qi
authors.initials: YQ
authors.lastname: Yang
mesh_terms: D015153:Blotting, Western; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055758:SOX9 Transcription Factor / Q000378:metabolism*
chemical_list: C568529:MIRN32 microRNA, human; D035683:MicroRNAs; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human