title: Eosinophil shape change and secretion.
abstract: The analysis of eosinophil shape change and mediator secretion is a useful tool in understanding how eosinophils respond to immunological stimuli and chemotactic factors. Eosinophils undergo dramatic shape changes, along with secretion of the granule-derived enzyme eosinophil peroxidase (EPX) in response to chemotactic stimuli including platelet-activating factor and CCL11 (eotaxin-1). Here, we describe the analysis of eosinophil shape change by confocal microscopy analysis and provide an experimental approach for comparing unstimulated cells with those that have been stimulated to undergo chemotaxis. In addition, we illustrate two different degranulation assays for EPX using OPD and an enzyme-linked immunosorbent assay technique and show how eosinophil degranulation may be assessed from in vitro as well as ex vivo stimulation.
authors.affiliation: Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, AB, Canada.
authors.forename: Lian
authors.initials: L
authors.lastname: Willetts
authors.forename: Sergei I
authors.initials: SI
authors.lastname: Ochkur
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Jacobsen
authors.forename: James J
authors.initials: JJ
authors.lastname: Lee
authors.forename: Paige
authors.initials: P
authors.lastname: Lacy
mesh_terms: D054413:Chemokine CCL11 / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects; D004804:Eosinophils / Q000166:cytology*; D006801:Humans; D018613:Microscopy, Confocal
chemical_list: D054413:Chemokine CCL11