title: Characterization of a proposed dichorhavirus associated with the citrus leprosis disease and analysis of the host response.
abstract: The causal agents of Citrus leprosis are viruses; however, extant diagnostic methods to identify them have failed to detect known viruses in orange, mandarin, lime and bitter orange trees with severe leprosis symptoms in Mexico, an important citrus producer. Using high throughput sequencing, a virus associated with citrus leprosis was identified, belonging to the proposed Dichorhavirus genus. The virus was termed Citrus Necrotic Spot Virus (CNSV) and contains two negative-strand RNA components; virions accumulate in the cytoplasm and are associated with plasmodesmata-channels interconnecting neighboring cells-suggesting a mode of spread within the plant. The present study provides insights into the nature of this pathogen and the corresponding plant response, which is likely similar to other pathogens that do not spread systemically in plants.
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Av. IPN 2508, Zacatenco 07360, México D.F., Mexico. lcruz@cinvestav.mx.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Cruz-Jaramillo
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Av. IPN 2508, Zacatenco 07360, México D.F., Mexico. rmedrano@cinvestav.mx.
authors.forename: Roberto
authors.initials: R
authors.lastname: Ruiz-Medrano
authors.affiliation: LaNSE, Centro de Investigación y de Estudios Avanzados del IPN Av. IPN 2508, Zacatenco 07360, México D.F., Mexico. mlrojas@cinvestav.mx.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Rojas-Morales
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Av. IPN 2508, Zacatenco 07360, México D.F., Mexico. abel.lopez@senasica.gob.mx.
authors.forename: José Abel
authors.initials: JA
authors.lastname: López-Buenfil
authors.affiliation: Servicio Nacional de Sanidad Inocuidad y Calidad Agroalimentaria, Guillermo Pérez Valenzuela 127, Coyoacán 04100, México D.F., Mexico. oscar.morales@senasica.gob.mx.
authors.forename: Oscar
authors.initials: O
authors.lastname: Morales-Galván
authors.affiliation: Servicio Nacional de Sanidad Inocuidad y Calidad Agroalimentaria, Guillermo Pérez Valenzuela 127, Coyoacán 04100, México D.F., Mexico. cchavap@gmail.com.
authors.forename: Claudio
authors.initials: C
authors.lastname: Chavarín-Palacio
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Av. IPN 2508, Zacatenco 07360, México D.F., Mexico. jarp1985@hotmail.com.
authors.forename: José Abrahán
authors.initials: JA
authors.lastname: Ramírez-Pool
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Av. IPN 2508, Zacatenco 07360, México D.F., Mexico. bxoconos@cinvestav.mx.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Xoconostle-Cázares
mesh_terms: D002957:Citrus / Q000276:immunology / Q000821:virology*; D005638:Fruit / Q000276:immunology / Q000821:virology; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions; D008800:Mexico; D019251:Nucleocapsid / Q000235:genetics* / Q000648:ultrastructure; D010802:Phylogeny; D059828:Plant Cells / Q000276:immunology / Q000821:virology; D010935:Plant Diseases / Q000276:immunology / Q000821:virology*; D057865:Plant Immunity; D018515:Plant Leaves / Q000276:immunology / Q000821:virology; D010942:Plant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D031425:Plasmodesmata / Q000276:immunology / Q000821:virology; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure