title: Fabrication of biomimetically patterned surfaces and their application to probing plant-bacteria interactions.
abstract: We have developed a two-step replica molding method for rapid fabrication of biomimetically patterned plant surfaces (BPS) using polydimethylsiloxane (PDMS-BPS) and agarose (AGAR-BPS). Beyond providing multiple identical specimens that faithfully reproduce leaf surface microstructure, this approach also offers unique chemical, physical, and biological features. PDMS-BPS provide good structural durability for SEM examination, have surface wettability comparable to plant surfaces for coating development, and allow for real-time monitoring of biosynthesis through incorporation into microfluidic devices. AGAR-BPS are compatible with bacterial growth, recovery, and quantification, and enable investigation of the effects of surface topography on spatially varying survival and inactivation of Escherichia coli cells during biocide treatment. Further development and application of these biomimetically patterned surfaces to study (and possibly modify) other aspects of plant-bacteria interactions can provide insight into controlling pathogen contamination in a wide range of applications.
authors.affiliation: Environmental Microbial and Food Safety Lab, Agricultural Research Service, United States Department of Agriculture , Beltsville, Maryland 20705, United States.
authors.forename: Boce
authors.initials: B
authors.lastname: Zhang
authors.forename: Yaguang
authors.initials: Y
authors.lastname: Luo
authors.forename: Arne J
authors.initials: AJ
authors.lastname: Pearlstein
authors.forename: Jesse
authors.initials: J
authors.lastname: Aplin
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Gary R
authors.initials: GR
authors.lastname: Bauchan
authors.forename: Gregory F
authors.initials: GF
authors.lastname: Payne
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiangwu
authors.initials: X
authors.lastname: Nou
authors.forename: Patricia D
authors.initials: PD
authors.lastname: Millner
mesh_terms: D032701:Biomimetics / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D005434:Flow Cytometry; D050296:Microbial Viability; D008853:Microscopy; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects; D011312:Pressure; D012685:Sepharose / Q000737:chemistry; D018724:Spinacia oleracea / Q000187:drug effects / Q000382:microbiology*; D017155:Wettability
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D012685:Sepharose