title: GHIP in Streptococcus pneumoniae is involved in antibacterial resistance and elicits a strong innate immune response through TLR2 and JNK/p38MAPK.
abstract: Interaction between pneumococcal virulence factors and innate immune receptors triggers host responses via specific signaling pathways after infection. By generating a deficient mutant, we show here that, compared with the wild-type parent strain, glycosyl hydrolase 25 relating to invasion protein (GHIP) mutant strain was impaired in rapid dissemination into vessels and caused less severe inflammation in mice lungs. Further study demonstrated that the lack of this protein in Streptococcus pneumoniae caused an increased susceptibility to whole blood or neutrophils, while this impairment could be recovered by supplementing recombinant GHIP (rGHIP). Additionally, secreted GHIP could be detected in culture medium, and purified protein was able to induce the release of tumor necrosis factor α and interleukin 6 from peritoneal macrophages. Further investigations revealed that the induction of interleukin 6 by this virulence factor depended on the phosphorylation of c-Jun N-terminal kinase and p38 mitogen activated protein kinase and Toll-like receptor 2. Taken together, GHIP, a novel pneumococcal virulence factor, appeared to play a critical role in bacterial survival and the induction of host innate immune response during pneumococcal infection.
authors.affiliation: Key Laboratory of Diagnostic Medicine designated by the Ministry of Education, Chongqing Medical University, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Yujuan
authors.initials: Y
authors.lastname: He
authors.forename: Chenwei
authors.initials: C
authors.lastname: Li
authors.forename: Aie
authors.initials: A
authors.lastname: Zhou
authors.forename: Jin
authors.initials: J
authors.lastname: Cui
authors.forename: Wenchun
authors.initials: W
authors.lastname: Xu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhong
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yin
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D007113:Immunity, Innate; D015850:Interleukin-6 / Q000096:biosynthesis; D048031:JNK Mitogen-Activated Protein Kinases / Q000502:physiology; D008168:Lung / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology; D010766:Phosphorylation; D011008:Pneumococcal Infections / Q000276:immunology / Q000382:microbiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000472:pathogenicity; D051195:Toll-Like Receptor 2 / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D037521:Virulence Factors / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D015850:Interleukin-6; D011994:Recombinant Proteins; D051195:Toll-Like Receptor 2; D014409:Tumor Necrosis Factor-alpha; D037521:Virulence Factors; C000592809:glycosyl hydrolase 25 relating to invasion protein, Streptococcus pneumoniae; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Streptococcus pneumoniae; Toll-like receptor 2; antibacterial resistance; glycosyl hydrolase 25; leukocyte recruitment