title: Comparison of clinical outcomes and genomic characteristics of single focus and multifocal glioblastoma.
abstract: We investigate the differences in molecular signature and clinical outcomes between multiple lesion glioblastoma (GBM) and single focus GBM in the modern treatment era. Between August 2000 and May 2010, 161 patients with GBM were treated with modern radiotherapy techniques. Of this group, 33 were considered to have multiple lesion GBM (25 multifocal and 8 multicentric). Patterns of failure, time to progression and overall survival were compared based on whether the tumor was considered a single focus or multiple lesion GBM. Genomic groupings and methylation status were also investigated as a possible predictor of multifocality in a cohort of 41 patients with available tissue for analysis. There was no statistically significant difference in overall survival (p < 0.3) between the multiple lesion tumors (8.2 months) and single focus GBM (11 months). Progression free survival was superior in the single focus tumors (7.1 months) as compared to multi-focal (5.6 months, p = 0.02). For patients with single focus, multifocal and multicentric GBM, 81, 76 and 88 % of treatment failures occurred in the 60 Gy volume (p < 0.5), while 54, 72, and 38 % of treatment failures occurred in the 46 Gy volume (p < 0.4). Out of field failures were rare in both single focus and multiple foci GBM (7 vs 3 %). Genomic groupings and methylation status were not found to predict for multifocality. Patterns of failure, survival and genomic signatures for multiple lesion GBM do not appreciably differ when compared to single focus tumors.
authors.affiliation: Wake Forest School of Medicine, Medical Center Blvd, Winston-Salem, NC, 27157, USA.
authors.forename: Anna K
authors.initials: AK
authors.lastname: Paulsson
authors.forename: Jordan A
authors.initials: JA
authors.lastname: Holmes
authors.forename: Ann M
authors.initials: AM
authors.lastname: Peiffer
authors.forename: Lance D
authors.initials: LD
authors.lastname: Miller
authors.forename: Wennuan
authors.initials: W
authors.lastname: Liu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xu
authors.forename: William H
authors.initials: WH
authors.lastname: Hinson
authors.forename: Glenn J
authors.initials: GJ
authors.lastname: Lesser
authors.forename: Adrian W
authors.initials: AW
authors.lastname: Laxton
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Tatter
authors.forename: Waldemar
authors.initials: W
authors.lastname: Debinski
authors.forename: Michael D
authors.initials: MD
authors.lastname: Chan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001932:Brain Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy*; D015331:Cohort Studies; D019175:DNA Methylation; D018572:Disease-Free Survival; D005260:Female; D005909:Glioblastoma / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D014057:Tomography, X-Ray Computed; D055815:Young Adult