title: Characterization and expression profiles of MaACS and MaACO genes from mulberry (Morus alba L.).
abstract: 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type I, MaACS3 and MaACS4 are type II, and MaACS5 is type III, with different C-terminal sequences. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demonstrated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid (ABA) and ethephon.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China.
authors.forename: Chang-ying
authors.initials: CY
authors.lastname: Liu
authors.forename: Rui-hua
authors.initials: RH
authors.lastname: Lü
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Ai-chun
authors.initials: AC
authors.lastname: Zhao
authors.forename: Xi-ling
authors.initials: XL
authors.lastname: Wang
authors.forename: Umuhoza
authors.initials: U
authors.lastname: Diane
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Wang
authors.forename: Chuan-hong
authors.initials: CH
authors.lastname: Wang
authors.forename: Ya-sheng
authors.initials: YS
authors.lastname: Yu
authors.forename: Shu-mei
authors.initials: SM
authors.lastname: Han
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
authors.forename: Mao-de
authors.initials: MD
authors.lastname: Yu
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D008190:Lyases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D031625:Morus / Q000201:enzymology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase
keywords: Abscisic acid; Ethephon; Gene expression; MaACO; MaACS; Mulberry fruit