title: Contamination of absorbent paper points in clinical practice: a critical approach.
abstract: This study sought to evaluate the contamination level of absorbent paper points used routinely in dental clinical practice. For this study, 60 absorbent paper points were collected and separated into 3 groups: 20 paper points from sealed commercial packages (Group 1), 20 paper points from open commercial packages in use for 30 days (Group 2), and 20 paper points from a sealed commercial package that were manipulated by the operator (Group 3). Evaluation criteria was the presence or absence of turbidity in the brain heart infusion (BHI) broth used as the culture medium. The results (Kruskal-Wallis test; significance level = 5%) demonstrated bacterial growth in most of the samples for all groups, with a statistically significant difference in Group 3 compared to Groups 1 and 2. It was concluded that inadequate manipulation of paper points by the operator caused these materials to become contaminated; in addition, the bacterial growth in absorbent paper points that are still in their commercial packages indicates the importance of sterilization before the paper points are used in clinical practice.
authors.forename: Larissa
authors.initials: L
authors.lastname: Pessoa de Andrade
authors.forename: Nikeila
authors.initials: N
authors.lastname: Chacon de Oliveira Conde
authors.forename: Emilio Carlos
authors.initials: EC
authors.lastname: Sponchiado Junior
authors.forename: Andre Augusto
authors.initials: AA
authors.lastname: Franco Marques
authors.forename: Juliana Vianna
authors.initials: JV
authors.lastname: Pereira
authors.forename: Lucas Fonseca Roberti
authors.initials: LF
authors.lastname: Garcia
mesh_terms: D003470:Culture Media; D004866:Equipment Contamination; D010209:Paper
chemical_list: D003470:Culture Media
keywords: absorbent paper points; contamination; endodontics