title: Implication of intracellular localization of transcriptional repressor PLZF in thyroid neoplasms.
abstract: BACKGROUND
  Promyelocytic leukaemia zinc finger (PLZF) is a transcriptional repressor that was originally isolated from a patient with promyelocytic leukaemia. PLZF also affects key elements for cell cycle progression, such as cyclin A, and can affect the tumourigenicity of various cancers. Thus far, the behaviour of PLZF in thyroid carcinoma remains unclear.
  
  
  METHODS
  We analysed the expression profile of PLZF in different types of benign and malignant thyroid lesions as well as in normal thyroid tissue. Specifically, we examined PLZF expression in normal thyroid (N; n = 4), adenomatous lesion (AL; n = 5), follicular adenoma (FA; n = 2), papillary thyroid carcinoma (PTC; n = 20), and anaplastic thyroid carcinoma (ATC; n = 3) samples. PLZF expression was estimated by western blotting and immunohistochemical (IHC) staining.
  
  
  RESULTS
  PLZF was expressed in all samples of thyroid lesions examined. In N, AL, and FA, PLZF was mainly localized in the nucleus. In contrast, in PTC and ATC, PLZF was mainly expressed in the cytosol with high intensity. In more detail, the cytoplasmic IHC scores in PTC with capsular invasion (CI) and lymph node (LN) metastasis were higher than those in PTC without CI and LN metastasis.
  
  
  CONCLUSIONS
  PLZF shows different subcellular localizations among PTC, ATC, and other thyroid lesions. Furthermore, high cytoplasmic expression of PLZF may be correlated with CI and LN metastasis in thyroid carcinoma. The present report is the first to describe the implications of intracellular PLZF expression in thyroid carcinomas.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Matsuzawa
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Izawa
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ohkura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ohkura
authors.forename: Kiyosuke
authors.initials: K
authors.lastname: Ishiguro
authors.forename: Akio
authors.initials: A
authors.lastname: Yoshida
authors.forename: Yumi
authors.initials: Y
authors.lastname: Takiyama
authors.forename: Masakazu
authors.initials: M
authors.lastname: Haneda
authors.forename: Chiaki
authors.initials: C
authors.lastname: Shigemasa
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Department of Regional Medicine, Tottori University Faculty of Medicine, 86 Nishi-cho, Yonago 683-8503, Japan. stani@med.tottori-u.ac.jp.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Taniguchi
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002291:Carcinoma, Papillary / Q000378:metabolism* / Q000556:secondary; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D013961:Thyroid Gland / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D051741:Kruppel-Like Transcription Factors; D000075722:Promyelocytic Leukemia Zinc Finger Protein; C092037:ZBTB16 protein, human