title: CpG-ODNs induced changes in cytokine/chemokines genes expression associated with suppression of infectious bronchitis virus replication in chicken lungs.
abstract: The process of virus replication in host cells is greatly influenced by the set of cytokines, chemokines and antiviral substances activated as a result of host-virus interaction. Alteration of cytokines profiles through manipulation of the innate immune system by innate immune stimulants may be helpful in inhibiting virus replication in otherwise permissive cells. The aim of present studies was to characterize innate immune responses capable of inhibiting infectious bronchitis virus (IBV) replication in chicken lungs after in ovo administration of CpG ODN. In our experiments, CpG ODN 2007 or PBS solution was injected on 18th embryonic day (ED) via the chorioallontoic route. CpG ODN and PBS inoculated embryos were challenged with virulent IBV on the 19th ED. Lung tissue samples from experimental chicks were analysed for cytokines/chemokines gene expression at 24h, 48h, and 72h, post infection. Our data showed significant differential up-regulation of IFN-γ, IL-8 (CXCLi2) and MIP-1β genes and suppression of IL-6 gene expression being associated with inhibition of IBV replication in lungs tissue retrieved from embryos pre-treated with CpG ODN. It is expected that understanding of the innate immune modulation of target tissues by the virus and innate immune stimulants will be helpful in identification of valuable targets for development of novel, safe, effective and economical control strategies against IBV infection in chickens.
authors.affiliation: Vaccine and Infectious Disease Organization-International Vaccine Center, University of Saskatchewan, SK, Saskatoon, SK, Canada S7N 5E3. Electronic address: Arshud.Dar@usask.ca.
authors.forename: Arshud
authors.initials: A
authors.lastname: Dar
authors.affiliation: Vaccine and Infectious Disease Organization-International Vaccine Center, University of Saskatchewan, SK, Saskatoon, SK, Canada S7N 5E3.
authors.forename: Suresh
authors.initials: S
authors.lastname: Tikoo
authors.affiliation: Vaccine and Infectious Disease Organization-International Vaccine Center, University of Saskatchewan, SK, Saskatoon, SK, Canada S7N 5E3.
authors.forename: Andy
authors.initials: A
authors.lastname: Potter
authors.affiliation: University of Alberta, 2-51 South Academic Building, Edmonton, AB, Canada T6G 2G7.
authors.forename: Lorne A
authors.initials: LA
authors.lastname: Babiuk
authors.affiliation: Vaccine and Infectious Disease Organization-International Vaccine Center, University of Saskatchewan, SK, Saskatoon, SK, Canada S7N 5E3.
authors.forename: Hugh
authors.initials: H
authors.lastname: Townsend
authors.affiliation: Vaccine and Infectious Disease Organization-International Vaccine Center, University of Saskatchewan, SK, Saskatoon, SK, Canada S7N 5E3.
authors.forename: Volker
authors.initials: V
authors.lastname: Gerdts
authors.affiliation: Vaccine and Infectious Disease Organization-International Vaccine Center, University of Saskatchewan, SK, Saskatoon, SK, Canada S7N 5E3.
authors.forename: George
authors.initials: G
authors.lastname: Mutwiri
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics; D054407:Chemokine CCL4 / Q000235:genetics; D018925:Chemokines / Q000235:genetics*; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D018899:CpG Islands; D016207:Cytokines / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral; D007113:Immunity, Innate / Q000235:genetics; D001351:Infectious bronchitis virus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D007371:Interferon-gamma / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D008168:Lung / Q000276:immunology / Q000821:virology; D019590:Nucleocapsid Proteins / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000235:genetics / Q000276:immunology
chemical_list: D030161:Avian Proteins; C408982:CPG-oligonucleotide; D054407:Chemokine CCL4; D018925:Chemokines; D016207:Cytokines; D016209:Interleukin-8; D019590:Nucleocapsid Proteins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D007371:Interferon-gamma
keywords: Avian infectious bronchitis virus; CpG ODN cytokines/chemokines; Inhibition of virus replication