title: [Study on oral absorption enhancers of astragalus polysaccharides].
abstract: Astragalus polysaccharides was lounded to 4-(2-aminoethylphenol), followed by labeling the APS-Tyr with fluorescein-5-isothiocyanate (FITC) at the secondary amino group. The absorption enhancement effects of low molecular weight chitosan and protamine on astragalus polysaccharides were evaluated via Caco-2 cell culture model. The results show that the fluorecent labeling compound has good stability and high sensitivity. On the other hand low molecular weight chitosan and protamine also can promoted absorption of the astragalus polysaccharides without any cytotoxity, and the absorption increase was more significant with increasing the amount of low molecular weight chitosan and protamine. At the same time, the low molecular weight chitosan has slightly better effect. The transepithelial electric resistance (TEER) of Caco-2 cells show that absorption enhancers could improve its membrane transport permeability by opening tight junctions between cells and increasing the cell membrane fluidity.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Chen
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Tan
authors.forename: E
authors.initials: E
authors.lastname: Sun
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Jia
authors.forename: Zhen-Hai
authors.initials: ZH
authors.lastname: Zhang
mesh_terms: D000042:Absorption; D027883:Astragalus Plant / Q000737:chemistry*; D001692:Biological Transport; D018938:Caco-2 Cells; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000493:pharmacokinetics*; D011134:Polysaccharides / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D005456:Fluorescent Dyes; D010936:Plant Extracts; D011134:Polysaccharides; D016650:Fluorescein-5-isothiocyanate