title: Hydrogen sulfide detection based on reflection: from a poison test approach of ancient China to single-cell accurate localization.
abstract: With the inspiration of an ancient Chinese poison test approach, we report a rapid hydrogen sulfide detection strategy in specific areas of live cells using silver needles with good spatial resolution of 2 × 2 μm(2). Besides the accurate-localization ability, this reflection-based strategy also has attractive merits of convenience and robust response when free pretreatment and short detection time are concerned. The success of endogenous H2S level evaluation in cellular cytoplasm and nuclear of human A549 cells promises the application potential of our strategy in scientific research and medical diagnosis.
authors.affiliation: Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Center for Atomic and Molecular Nanosciences, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Kong
authors.forename: Zhuoran
authors.initials: Z
authors.lastname: Ma
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Gong
authors.forename: Sichun
authors.initials: S
authors.lastname: Zhang
authors.forename: Xinrong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002681:China; D056945:Hep G2 Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D009339:Needles; D059010:Single-Cell Analysis
chemical_list: D006862:Hydrogen Sulfide