title: Genetic diversity in Enterocytozoon bieneusi isolates from dogs and cats in China: host specificity and public health implications.
abstract: To explore the genetic diversity, host specificity, and zoonotic potential of Enterocytozoon bieneusi, feces from 348 stray and pet dogs and 96 pet cats from different locations in China were examined by internal transcribed spacer (ITS)-based PCR. E. bieneusi was detected in 15.5% of the dogs, including 20.5% of stray dogs and 11.7% of pet dogs, and in 11.5% of the pet cats. Higher infection rates were recorded in the >2-year and the 1- to 2-year age groups in dogs and cats, respectively. Altogether, 24 genotypes, including 11 known and 13 new, were detected in 65 infected animals. In 54 positive dogs, 18 genotypes, 9 known (PtEbIX, O, D, CM1, EbpA, Peru8, type IV, EbpC, and PigEBITS5) and 9 new (CD1 to CD9), were found. In contrast, 8 genotypes, 4 known (D, BEB6, I, and PtEbIX) and 4 new (CC1 to CC4), were identified in 11 infected cats. The dominant genotype in dogs was PtEbIX (26/54). Phylogenetic analysis revealed that 8 known genotypes (D, Peru8, type IV, CM1, EbpC, PigEBITS5, O, and EbpA) and 7 new genotypes (CD1 to CD4 and CC2 to CC4) were the members of zoonotic group 1, whereas genotypes CD7, CD8, and CD9 together with PtEbIX belonged to the dog-specific group, and genotypes CD6 and CC1 were placed in group 2 with BEB6 and I. Conversely, genotype CD5 clustered with CM4 without belonging to any previous groups. We conclude that zoonotic genotypes are common in dogs and cats, as are host-specific genotypes in dogs.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
authors.forename: Md Robiul
authors.initials: MR
authors.lastname: Karim
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
authors.forename: Haiju
authors.initials: H
authors.lastname: Dong
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
authors.forename: Fuchang
authors.initials: F
authors.lastname: Yu
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
authors.forename: Fuchun
authors.initials: F
authors.lastname: Jian
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China zhanglx8999@gmail.com lxiao@cdc.gov.
authors.forename: Longxian
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
authors.forename: Rongjun
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Patuakhali Science and Technology University, Patuakhali, Bangladesh.
authors.forename: Farzana Islam
authors.initials: FI
authors.lastname: Rume
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
authors.forename: Changshen
authors.initials: C
authors.lastname: Ning
authors.affiliation: Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA zhanglx8999@gmail.com lxiao@cdc.gov.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000367:Age Factors; D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000382:microbiology*; D002415:Cats; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology / Q000382:microbiology*; D004285:Dogs; D021862:Enterocytozoon / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D008297:Male; D016881:Microsporidiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer