title: The effect of anthocyans on the expression of selected phase II xenobiotic-metabolizing enzymes in primary cultures of human hepatocytes.
abstract: Anthocyans are biologically active constituents of various berry fruits and they are also contained in nutritional supplements derived from extracts or dry matter from berry fruits. In this study we evaluated the effects of anthocyans on the expression of selected drug-metabolizing phase II genes in primary cultures of human hepatocytes by qRT-PCR. Most of the tested anthocyanidins (6) and anthocyanins (21) did not induce the expression of mRNA of UGT1A/2B members in human hepatocytes. The same can be stated for expression of selected GST genes on the mRNA level. However, some of them e.g. cyanidin-3-O-rutinoside consistently decreased the level of GSTP1 mRNA in all tested cultures. In conclusion, most of the anthocyans did not affect the expression of selected phase II metabolizing enzymes in vitro.
authors.affiliation: Department of Cell Biology and Genetics, Faculty of Science, Palacky University, Slechtitelu 11, 783 71 Olomouc, Czech Republic. radim.vrzal@email.cz.
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Dvorak
authors.forename: Alzbeta
authors.initials: A
authors.lastname: Srovnalova
authors.forename: Michaela
authors.initials: M
authors.lastname: Svecarova
authors.forename: Radim
authors.initials: R
authors.lastname: Vrzal
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology*; D002478:Cells, Cultured; D014453:Glucuronosyltransferase / Q000235:genetics / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D050217:Metabolic Detoxication, Phase II; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D015262:Xenobiotics; D014453:Glucuronosyltransferase