title: Two-photon imaging of neural activity in awake mobile mice.
abstract: A method for functional, cellular-resolution imaging of neural populations in awake and mobile mice is presented here. The method is based on the use of a spherical treadmill, head restraint, and motion correction software that facilitate neural imaging in the awake brain with a fixed upright two-photon microscope. These approaches have proven to be applicable to a wide range of brain regions and should help further our understanding of how neuronal population activity within the brain's microcircuitry is connected to animal behavior.
authors.forename: Daniel
authors.initials: D
authors.lastname: Dombeck
authors.forename: David
authors.initials: D
authors.lastname: Tank
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods*; D005080:Exercise Test; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D009474:Neurons / Q000502:physiology*; D017785:Photons; D014851:Wakefulness