title: Antinociceptive effect of intrathecal microencapsulated human pheochromocytoma cell in a rat model of bone cancer pain.
abstract: Human pheochromocytoma cells, which are demonstrated to contain and release met-enkephalin and norepinephrine, may be a promising resource for cell therapy in cancer-induced intractable pain. Intrathecal injection of alginate-poly (l) lysine-alginate (APA) microencapsulated human pheochromocytoma cells leads to antinociceptive effect in a rat model of bone cancer pain, and this effect was blocked by opioid antagonist naloxone and alpha 2-adrenergic antagonist rauwolscine. Neurochemical changes of cerebrospinal fluid are in accordance with the analgesic responses. Taken together, these data support that human pheochromocytoma cell implant-induced antinociception was mediated by met-enkephalin and norepinephrine secreted from the cell implants and acting at spinal receptors. Spinal implantation of microencapsulated human pheochromocytoma cells may provide an alternative approach for the therapy of chronic intractable pain.
authors.affiliation: Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. lixiao204011987@sina.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cardiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. liguoqi09@163.com.
authors.forename: Guoqi
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. 13660183777@126.com.
authors.forename: Shaoling
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. baiyuzsu@yahoo.com.cn.
authors.forename: Baiyu
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. soswq@hotmail.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. 13654456833@163.com.
authors.forename: Ding
authors.initials: D
authors.lastname: Yu
authors.affiliation: Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. zhouruijun6666@163.com.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. ma_chao99@126.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials; D001859:Bone Neoplasms / Q000150:complications; D017690:Cell Transplantation; D004745:Enkephalins / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D009270:Naloxone / Q000494:pharmacology; D059226:Nociceptive Pain / Q000209:etiology / Q000628:therapy*; D009638:Norepinephrine / Q000134:cerebrospinal fluid; D010673:Pheochromocytoma / Q000378:metabolism* / Q000473:pathology; D011107:Polylysine / Q000031:analogs & derivatives / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D015016:Yohimbine / Q000494:pharmacology
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D004745:Enkephalins; C051546:alginate-polylysine-alginate; D011107:Polylysine; D015016:Yohimbine; D009270:Naloxone; D009638:Norepinephrine