title: Functional mapping of the auditory tract in rodent tinnitus model using manganese-enhanced magnetic resonance imaging.
abstract: Animal models of salicylate-induced tinnitus have demonstrated that salicylate modulates neuronal activity in several brain structures leading to neuronal hyperactivity in auditory and non-auditory brain areas. In addition, these animal tinnitus models indicate that tinnitus can be a perceptual consequence of altered spontaneous neural activity along the auditory pathway. Peripheral and/or central effects of salicylate can account for neuronal activity changes in salicylate-induced tinnitus. Because of this ambiguity, an in vivo imaging study would be able to address the peripheral and/or central involvement of salicylate-induced tinnitus. Therefore, in the present study, we developed a novel manganese-enhanced magnetic resonance imaging (MEMRI) method to map the in vivo functional auditory tract in a salicylate-induced tinnitus animal model by administrating manganese through the round window. We found that acute salicylate-induced tinnitus resulted in higher manganese uptake in the cochlea and in the central auditory structures. Furthermore, serial MRI scans demonstrated that the manganese signal increased in an anterograde fashion from the cochlea to the cochlear nucleus. Therefore, our in vivo MEMRI data suggest that acute salicylate-induced tinnitus is associated with higher spontaneous neural activity both in peripheral and central auditory pathways.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors.forename: Da Jung
authors.initials: DJ
authors.lastname: Jung
authors.affiliation: Department of Medical and Biological Engineering, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors.forename: Mun
authors.initials: M
authors.lastname: Han
authors.affiliation: Department of Medical and Biological Engineering, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors.forename: Seong-Uk
authors.initials: SU
authors.lastname: Jin
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Daegu Veterans Hospital, Daegu, Republic of Korea.
authors.forename: Sang Heun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, College of Medicine, Dankook University, Cheonan, Republic of Korea.
authors.forename: Ilyong
authors.initials: I
authors.lastname: Park
authors.affiliation: Department of Biology, College of Natural Science, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Cho
authors.affiliation: Department of Biology, College of Natural Science, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Tae-Jun
authors.initials: TJ
authors.lastname: Kwon
authors.affiliation: Department of Radiology, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors.forename: Hui Joong
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Electronic Engineering, College of IT Engineering, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea. Electronic address: kylee@knu.ac.kr.
authors.forename: Kyu-Yup
authors.initials: KY
authors.lastname: Lee
authors.affiliation: Department of Medical and Biological Engineering, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea; Department of Radiology, School of Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea; Department of Molecular Medicine, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University, Daegu, Republic of Korea. Electronic address: ychang@knu.ac.kr.
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Chang
mesh_terms: D000818:Animals; D001306:Auditory Pathways / Q000503:physiopathology; D003051:Cochlea / Q000503:physiopathology*; D003056:Cochlear Nerve / Q000503:physiopathology*; D017626:Cochlear Nucleus / Q000503:physiopathology*; D004195:Disease Models, Animal; D007089:Image Enhancement; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008345:Manganese; D051381:Rats; D017207:Rats, Sprague-Dawley; D014012:Tinnitus / Q000503:physiopathology*
chemical_list: D008345:Manganese
keywords: Auditory pathway; Gap prepulse inhibition of acoustic startle; Manganese-enhanced MRI; Salicylate; Tinnitus