title: Rhodoligotrophos jinshengii sp. nov., isolated from activated sludge.
abstract: A Gram-stain-negative, non-spore-forming, non-motile, ovoid, aerobic bacterial strain, designated BUT-3(T), was isolated from activated sludge from the wastewater treatment facility of a herbicide-manufacturing plant in Kunshan city, Jiangsu province, PR China. Strain BUT-3(T) grew between 15 and 40 °C, with optimum growth at 30 °C. The pH range for growth was between 5.0 and 10.0 (optimum pH 7.0). The range of NaCl concentrations for growth of strain BUT-3(T) was 0-7.0 % (w/v), with an optimum of 1.5-3.0 % (w/v). A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain BUT-3(T) clustered closely with Rhodoligotrophos appendicifer 120-1(T) (98.32 % similarity), with a bootstrap confidence level of 100 %. The major fatty acids (>5 % of total fatty acids) were C19 : 0 cyclo ω8c, C18 : 1ω7c, C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. Strain BUT-3(T) contained ubiquinone Q-10 as the predominant respiratory quinone. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, three unidentified aminolipids (AL1-3), two unknown phospholipids (PL1, 5), four unidentified glycolipids (GL1-4) and two unknown lipids (L1, 2). The G+C content of the genomic DNA was 67.7 mol%. The DNA-DNA relatedness between BUT-3(T) and R. appendicifer 120-1(T) was 44.1±0.6 %. Based on the polyphasic taxonomic data, strain BUT-3(T) should be classified as a representative of a novel species of the genus Rhodoligotrophos, for which the name Rhodoligotrophos jinshengii sp. nov. is proposed. The type strain is BUT-3(T) ( = CCTCC AB2013083(T) = KACC 17220(T)).
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shi-Kai
authors.initials: SK
authors.lastname: Deng
authors.affiliation: Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing, 210001, PR China.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shu
authors.initials: S
authors.lastname: Cai
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Yao
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014451:Ubiquinone / Q000737:chemistry; D062065:Wastewater
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014451:Ubiquinone; D062065:Wastewater; C025204:Ubiquinone Q2