title: Artificial neural network optimization of Althaea rosea seeds polysaccharides and its antioxidant activity.
abstract: A combination of an orthogonal L16(4)4 test design and a three-layer artificial neural network (ANN) model was applied to optimize polysaccharides from Althaea rosea seeds extracted by hot water method. The highest optimal experimental yield of A. rosea seed polysaccharides (ARSPs) of 59.85 mg/g was obtained using three extraction numbers, 113 min extraction time, 60.0% ethanol concentration, and 1:41 solid-liquid ratio. Under these optimized conditions, the ARSP experimental yield was very close to the predicted yield of 60.07 mg/g and was higher than the orthogonal test results (40.86 mg/g). Structural characterizations were conducted using physicochemical property and FTIR analysis. In addition, the study of ARSP antioxidant activity demonstrated that polysaccharides exhibited high superoxide dismutase activity, strong reducing power, and positive scavenging activity on superoxide anion, hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl, and reducing power. Our results indicated that ANNs were efficient quantitative tools for predicting the total ARSP content.
authors.affiliation: College of Chemistry and Chemical Engineering, Xinjiang Normal University, Xinjiang 830054, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Information Sciences and Technology, Xinjiang Education Institute, Xinjiang 830043, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Liu
authors.affiliation: Central Laboratory of Xinjiang Medical University, Xinjiang 830011, China. Electronic address: tsgyz@sina.com.
authors.forename: Shuge
authors.initials: S
authors.lastname: Tian
mesh_terms: D031583:Althaea / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D016571:Neural Networks, Computer; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000037:antagonists & inhibitors
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical; D013482:Superoxide Dismutase
keywords: Althaea rosea seed; Antioxidant activities; Artificial neural network model; Orthogonal test design; Polysaccharide