title: Effect of ultrasound on the isoflavonoid production in Genista tinctoria L. suspension cultures.
abstract: BACKGROUND
  Application of ultrasound (US) to biotechnology is relatively new but several processes that take place in the presence of cells or enzymes are activated by ultrasonic waves. Genista tinctoria L. (Fabaceae) is rich on various kind of flavonoids, including isoflavones with valuable estrogenic activity.
  
  
  OBJECTIVE
  This study verified use of low-energy US elicitor to enhance secondary metabolite production in plant cell cultures.
  
  
  METHODS
  Suspension cultures of G. tinctoria cells was exposed to low-power US (with fixed frequency 35 kHz and power level 0.1 mW/cm(3)) for period 1-5 min.
  
  
  RESULTS
  The US exposure significantly stimulated genistin content (0.8 mg/g DW) after 3 min of US treatment (sampled after 72 h). The highest daidzein level (1.4 mg/g DW) was reached after US irradiation for 5 min and 168 h sampling.
  
  
  CONCLUSIONS
  The achieved results suggest that US can act as a potent abiotic elicitor to induce the defense responses of plant cells and to stimulate secondary metabolite production in plant cell cultures.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy in Hradec Králové, Charles University Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Tůmová
authors.affiliation: Department of Biology, University of Hradec Králové, Faculty of Natural Sciences, Rokitanského 62 500 03 Hradec Králové, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Tůma
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy in Hradec Králové, Charles University Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Hendrychová
keywords: Daidzein; Genista tinctoria; genistin; suspension; ultrasound