title: In tobacco BY-2 cells xyloglucan oligosaccharides alter the expression of genes involved in cell wall metabolism, signalling, stress responses, cell division and transcriptional control.
abstract: Xyloglucan oligosaccharides (XGOs) are breakdown products of XGs, the most abundant hemicelluloses of the primary cell walls of non-Poalean species. Treatment of cell cultures or whole plants with XGOs results in accelerated cell elongation and cell division, changes in primary root growth, and a stimulation of defence responses. They may therefore act as signalling molecules regulating plant growth and development. Previous work suggests an interaction with auxins and effects on cell wall loosening, however their mode of action is not fully understood. The effect of an XGO extract from tamarind (Tamarindus indica) on global gene expression was therefore investigated in tobacco BY-2 cells using microarrays. Over 500 genes were differentially regulated with similar numbers and functional classes of genes up- and down-regulated, indicating a complex interaction with the cellular machinery. Up-regulation of a putative XG endotransglycosylase/hydrolase-related (XTH) gene supports the mechanism of XGO action through cell wall loosening. Differential expression of defence-related genes supports a role for XGOs as elicitors. Changes in the expression of genes related to mitotic control and differentiation also support previous work showing that XGOs are mitotic inducers. XGOs also affected expression of several receptor-like kinase genes and transcription factors. Hence, XGOs have significant effects on expression of genes related to cell wall metabolism, signalling, stress responses, cell division and transcriptional control.
authors.affiliation: Plant Biology Department, Faculty of Biology, University of Havana, Havana City, Cuba.
authors.forename: Lien
authors.initials: L
authors.lastname: González-Pérez
authors.forename: Lara
authors.initials: L
authors.lastname: Perrotta
authors.forename: Alexis
authors.initials: A
authors.lastname: Acosta
authors.forename: Esteban
authors.initials: E
authors.lastname: Orellana
authors.forename: Natasha
authors.initials: N
authors.lastname: Spadafora
authors.forename: Leonardo
authors.initials: L
authors.lastname: Bruno
authors.forename: Beatrice M
authors.initials: BM
authors.lastname: Bitonti
authors.forename: Diego
authors.initials: D
authors.lastname: Albani
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Cabrera
authors.forename: Dennis
authors.initials: D
authors.lastname: Francis
authors.forename: Hilary J
authors.initials: HJ
authors.lastname: Rogers
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002473:Cell Wall / Q000502:physiology*; D002478:Cells, Cultured; D042002:Chromatin Assembly and Disassembly; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005936:Glucans / Q000378:metabolism* / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013312:Stress, Physiological; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D014990:Xylans / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D005936:Glucans; D006657:Histones; D014990:Xylans; C029353:xyloglucan