title: Twinkle, twinkle little star: photoswitchable fluorophores for super-resolution imaging.
abstract: Photoswitchable fluorescent probes are key elements of newly developed super-resolution fluorescence microscopy techniques that enable far-field interrogation of biological systems with a resolution of 50 nm or better. In contrast to most conventional fluorescence imaging techniques, the performance achievable by most super-resolution techniques is critically impacted by the photoswitching properties of the fluorophores. Here we review photoswitchable fluorophores for super-resolution imaging with discussion of the fundamental principles involved, a focus on practical implementation with available tools, and an outlook on future directions.
authors.affiliation: Department of Chemistry, University of Washington, Seattle, WA, USA.
authors.forename: Tyler J
authors.initials: TJ
authors.lastname: Chozinski
authors.affiliation: Department of Chemistry, University of Washington, Seattle, WA, USA.
authors.forename: Lauren A
authors.initials: LA
authors.lastname: Gagnon
authors.affiliation: Department of Chemistry, University of Washington, Seattle, WA, USA; Department of Physiology and Biophysics, University of Washington, Seattle, WA, USA. Electronic address: jcv2@uw.edu.
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Vaughan
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009930:Organic Chemicals / Q000737:chemistry; D055668:Photochemical Processes
chemical_list: D005456:Fluorescent Dyes; D009930:Organic Chemicals
keywords: Fluorophore; Photoswitch; Super-resolution imaging