title: Acetone utilization by sulfate-reducing bacteria: draft genome sequence of Desulfococcus biacutus and a proteomic survey of acetone-inducible proteins.
abstract: BACKGROUND
  The sulfate-reducing bacterium Desulfococcus biacutus is able to utilize acetone for growth by an inducible degradation pathway that involves a novel activation reaction for acetone with CO as a co-substrate. The mechanism, enzyme(s) and gene(s) involved in this acetone activation reaction are of great interest because they represent a novel and yet undefined type of activation reaction under strictly anoxic conditions.
  
  
  RESULTS
  In this study, a draft genome sequence of D. biacutus was established. Sequencing, assembly and annotation resulted in 159 contigs with 5,242,029 base pairs and 4773 predicted genes; 4708 were predicted protein-encoding genes, and 3520 of these had a functional prediction. Proteins and genes were identified that are specifically induced during growth with acetone. A thiamine diphosphate-requiring enzyme appeared to be highly induced during growth with acetone and is probably involved in the activation reaction. Moreover, a coenzyme B12- dependent enzyme and proteins that are involved in redox reactions were also induced during growth with acetone.
  
  
  CONCLUSIONS
  We present for the first time the genome of a sulfate reducer that is able to grow with acetone. The genome information of this organism represents an important tool for the elucidation of a novel reaction mechanism that is employed by a sulfate reducer in acetone activation.
authors.forename: Olga B
authors.initials: OB
authors.lastname: Gutiérrez Acosta
authors.forename: David
authors.initials: D
authors.lastname: Schleheck
authors.affiliation: Department of Biology and Konstanz Research School Chemical Biology, University of Konstanz, D-78457 Konstanz, Germany. bernhard.schink@uni-konstanz.de.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schink
mesh_terms: D000096:Acetone / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D020148:Butyric Acid / Q000378:metabolism; D020451:Contig Mapping; D020564:Deltaproteobacteria / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D058977:Molecular Sequence Annotation; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D020148:Butyric Acid; D000096:Acetone