title: Discordance of two interferon-γ release assays and tuberculin skin test in patients with uveitis.
abstract: OBJECTIVE
  To describe interferon-γ release assay (IGRA) and IGRA/tuberculin skin test (TST) discordance and their associated factors in patients with uveitis.
  
  
  METHODS
  Prospective study of 102 consecutive new patients presenting with uveitis to a tertiary referral centre. All patients underwent a complete ocular and systemic examination at presentation, with QuantiFERON-Gold in-tube (QFT), T-SPOT.TB and TST performed. Discordant results of TST/QFT, TST/T-SPOT.TB and QFT/T-SPOT.TB were analysed. Binary logistic regression was used to identify factors associated with discordant results.
  
  
  RESULTS
  There was fair agreement between TST and T-SPOT.TB (κ=0.371, p<0.001); moderate agreement between TST and QFT (κ=0.471, p<0.001). Both IGRAs showed good agreement (κ=0.668, p<0.001). The discordance rate between the TST/QFT was 26.5%, while TST/T-SPOT.TB was 31.4%. The discordance rate between the IGRAs was 16.7%. There was a significant association between TST/IGRA discordance with increasing age (OR 0.95, 95% CI 0.92 to 0.99; p=0.022), less likely with panuveitis (OR 0.18, 95% CI 0.04 to 0.86; p=0.032), and with Indian ethnicity (OR 14.07, 95% CI 1.45 to 137.03; p=0.023).
  
  
  CONCLUSIONS
  Discordance of IGRA and TST results is common and requires further study. In our study in patients with uveitis, we found that ethnicity, age, and type of uveitis were some significant factors associated with TST/IGRA discordance, which may need to be taken into consideration when interpreting these results.
authors.affiliation: Singapore National Eye Centre, Singapore, Singapore Singapore Eye Research Institute, Singapore, Singapore Department of Ophthalmology, Yong Yoo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
authors.forename: Marcus
authors.initials: M
authors.lastname: Ang
authors.affiliation: Singapore National Eye Centre, Singapore, Singapore.
authors.forename: Sieh Yean
authors.initials: SY
authors.lastname: Kiew
authors.affiliation: Singapore Eye Research Institute, Singapore, Singapore.
authors.forename: Wan Ling
authors.initials: WL
authors.lastname: Wong
authors.affiliation: Singapore National Eye Centre, Singapore, Singapore Singapore Eye Research Institute, Singapore, Singapore Department of Ophthalmology, Yong Yoo Lin School of Medicine, National University of Singapore, Singapore, Singapore Duke-National University of Singapore, Graduate Medical School, Singapore, Singapore.
authors.forename: Soon-Phaik
authors.initials: SP
authors.lastname: Chee
mesh_terms: D000328:Adult; D000368:Aged; D000995:Antitubercular Agents / Q000627:therapeutic use; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D059425:Interferon-gamma Release Tests; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D013902:Radiography, Thoracic; D015203:Reproducibility of Results; D014374:Tuberculin Test; D014392:Tuberculosis, Ocular / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D014605:Uveitis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D055815:Young Adult
chemical_list: D000995:Antitubercular Agents
keywords: Infection; Inflammation; Microbiology; Vitreous