title: Real-time label-free measurement of HIV-1 protease activity by nanopore analysis.
abstract: A label-free method for the measurement of the activity of HIV-1 protease is developed by real-time monitoring of the cleavage of a peptide substrate by HIV-1 protease in a nanopore. The method is rapid and sensitive: picomolar concentrations of HIV-1 protease could be detected in ~10 min. Simulated clinical samples are analyzed, and the activity of HIV-1 protease could be accurately detected. Our developed nanopore sensor design strategy should find useful applications in the development of stochastic sensors for other proteases of medical, pharmaceutical, and biological importance.
authors.affiliation: Department of Biological and Chemical Sciences, Illinois Institute of Technology, Chicago, IL 60616, United States.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Biological and Chemical Sciences, Illinois Institute of Technology, Chicago, IL 60616, United States.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Biological and Chemical Sciences, Illinois Institute of Technology, Chicago, IL 60616, United States.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Biological and Chemical Sciences, Illinois Institute of Technology, Chicago, IL 60616, United States. Electronic address: xguan5@iit.edu.
authors.forename: Xiyun
authors.initials: X
authors.lastname: Guan
mesh_terms: D000595:Amino Acid Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003199:Computer Systems; D016333:HIV Protease / Q000032:analysis* / Q000378:metabolism; D015497:HIV-1 / Q000201:enzymology / Q000302:isolation & purification; D006801:Humans; D058608:Nanopores; D009842:Oligopeptides / Q000737:chemistry; D059748:Proteolysis; D013379:Substrate Specificity
chemical_list: D009842:Oligopeptides; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1
keywords: Biosensors; HIV-1 protease; Nanopore; Peptide; Proteolysis