title: Viability, differentiation capacity, and detectability of super-paramagnetic iron oxide-labeled muscle precursor cells for magnetic-resonance imaging.
abstract: Cell therapies are a promising approach for the treatment of a variety of human conditions including stress urinary incontinence, but their success greatly depends on the biodistribution, migration, survival, and differentiation of the transplanted cells. Noninvasive in vivo cell tracking therefore presents an important aspect for translation of such a procedure into the clinics. Upon labeling with superparamagnetic iron oxide (SPIO) nanoparticles, cells can be tracked by magnetic resonance imaging (MRI), but possible adverse effect of the labeling have to be considered when labeling stem cells with SPIOs. In this study, human muscle precursor cells (hMPC) were labeled with increasing concentrations of SPIO nanoparticles (100-1600 μg/mL) and cell viability and differentiation capacity upon labeling was assessed in vitro. While a linear dependence between cell viability and nanoparticle concentration could be observed, differentiation capacity was not affected by the presence of SPIOs. Using a nude mouse model, a concentration (400 μg/mL) could be defined that allows reliable detection of hMPCs by MRI but does not influence myogenic in vivo differentiation to mature and functional muscle tissue. This suggests that such an approach can be safely used in a clinical setting to track muscle regeneration in patients undergoing cell therapy without negative effects on the functionality of the bioengineered muscle.
authors.affiliation: 1 Division of Urology, University Hospital Zurich , Zurich, Switzerland .
authors.forename: Fahd
authors.initials: F
authors.lastname: Azzabi
authors.forename: Markus
authors.initials: M
authors.lastname: Rottmar
authors.forename: Virginija
authors.initials: V
authors.lastname: Jovaisaite
authors.forename: Markus
authors.initials: M
authors.lastname: Rudin
authors.forename: Tullio
authors.initials: T
authors.lastname: Sulser
authors.forename: Andreas
authors.initials: A
authors.lastname: Boss
authors.forename: Daniel
authors.initials: D
authors.lastname: Eberli
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D003911:Dextrans / Q000378:metabolism*; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D008819:Mice, Nude; D009119:Muscle Contraction; D024510:Muscle Development; D009124:Muscle Proteins / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology* / Q000637:transplantation; D009928:Organ Specificity; D010641:Phenotype; D013194:Staining and Labeling
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D009124:Muscle Proteins; C065507:ferumoxides