title: Peptide-based electrochemical approach for apoptosis evaluation.
abstract: This paper reports a strategy to assemble apoptotic cells on a solid surface using a peptide as the recognition element. And a peptide-based electrochemical biosensor to directly evaluate apoptosis is described for the first time. The peptide modified on an electrode is designed to contain the sequence that can recognize externalized phosphatidylserine on apoptotic cells, and can then capture the cells onto the electrode surface. In the electrochemical system, the immobilized cells can not only provide significant steric hindrance for electron transfer, but also shield the positive charges of the peptide that can attract negatively charged electrochemical probes. Therefore, the obtained electrochemical signals drop significantly after the incubation of apoptotic cells, which can be used to reveal the apoptosis level. The experimental results of this approach are well in line with other standard methods. Moreover, this electrochemical method is simple, cost-effective, convenient, sensitive, and holds great potential toward apoptosis evaluation, therapeutic effect assessment and deeper cellular biological studies.
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: miaopeng@sibet.ac.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Miao
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China. Electronic address: yinj@sibet.ac.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
authors.forename: Limin
authors.initials: L
authors.lastname: Ning
authors.affiliation: Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006801:Humans; D061986:MCF-7 Cells; D010455:Peptides / Q000737:chemistry*; D010718:Phosphatidylserines / Q000032:analysis
chemical_list: D010455:Peptides; D010718:Phosphatidylserines
keywords: Apoptosis; Biosensor; Electrochemistry; Peptide; Phosphatidylserine