title: Antioxidative effect of epigallocatechin gallate against D-galactosamine-induced injury in primary culture of rat hepatocytes.
abstract: Literature data support that green tea and its major component epigallocatechin gallate (EGCG) have powerful antioxidant effects. Contrary, hepatotoxicity can be induced by high-dose EGCG. The timing of exposure to green tea in relation to administration of hepatotoxic agent plays an import role too. The aim of our work was a verification of antioxidative effect of EGCG on D-galactosamine-induced injury in primary culture of rat hepatocytes. Hepatocytes were incubated with EGCG at concentrations of 1.25-10 μM and toxic D-galactosamine (GalN) for 24 hrs. Alternatively, hepatocytes were pretreated with EGCG for 24 hrs, and then incubated with EGCG and GalN for further 24 hrs. Cytotoxicity was analysed by lactate dehydrogenase activity, functional capacity by albumin production. Oxidative stress was evaluated from a production of malondialdehyde and glutathione content in the cells. EGCG protected hepatocytes against GalN-induced cytotoxicity but preventive treatment of intact hepatocytes with EGCG was required to diminish the development of hepatocyte injury. Oxidative stress induced in our study seems to overcome the ability of hepatocytes to improve GSH depletion and albumin production. Prolongation of the pretreatment with EGCG could be a promising strategy leading to amelioration of its hepatoprotective effect.
authors.affiliation: Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of Physiology.
authors.forename: Alena
authors.initials: A
authors.lastname: Moravcova
authors.affiliation: Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of Physiology.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Cervinkova
authors.affiliation: Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of Physiology.
authors.forename: Otto
authors.initials: O
authors.lastname: Kucera
authors.affiliation: Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of Physiology.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Mezera
authors.affiliation: Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of Physiology.
authors.forename: Halka
authors.initials: H
authors.lastname: Lotkova
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D018929:Cell Culture Techniques; D005688:Galactosamine / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D051381:Rats
chemical_list: D000975:Antioxidants; D005688:Galactosamine; D002392:Catechin; C045651:epigallocatechin gallate; D005978:Glutathione
keywords: D-galactosamine; Epigallocatechin gallate; Hepatocytes; Oxidative stress