title: Treadmill training increases SIRT-1 and PGC-1 α protein levels and AMPK phosphorylation in quadriceps of middle-aged rats in an intensity-dependent manner.
abstract: The present study investigated the effects of running at 0.8 or 1.2 km/h on inflammatory proteins (i.e., protein levels of TNF- α , IL-1 β , and NF- κ B) and metabolic proteins (i.e., protein levels of SIRT-1 and PGC-1 α , and AMPK phosphorylation) in quadriceps of rats. Male Wistar rats at 3 (young) and 18 months (middle-aged rats) of age were divided into nonexercised (NE) and exercised at 0.8 or 1.2 km/h. The rats were trained on treadmill, 50 min per day, 5 days per week, during 8 weeks. Forty-eight hours after the last training session, muscles were removed, homogenized, and analyzed using biochemical and western blot techniques. Our results showed that: (a) running at 0.8 km/h decreased the inflammatory proteins and increased the metabolic proteins compared with NE rats; (b) these responses were lower for the inflammatory proteins and higher for the metabolic proteins in young rats compared with middle-aged rats; (c) running at 1.2 km/h decreased the inflammatory proteins and increased the metabolic proteins compared with 0.8 km/h; (d) these responses were similar between young and middle-aged rats when trained at 1.2 km. In summary, the age-related increases in inflammatory proteins, and the age-related declines in metabolic proteins can be reversed and largely improved by treadmill training.
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, Extremo Sul Catarinense University (UNESC), 88806-000 Criciuma, SC, Brazil.
authors.forename: Nara R C
authors.initials: NR
authors.lastname: Oliveira
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, Extremo Sul Catarinense University (UNESC), 88806-000 Criciuma, SC, Brazil.
authors.forename: Scherolin O
authors.initials: SO
authors.lastname: Marques
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, Extremo Sul Catarinense University (UNESC), 88806-000 Criciuma, SC, Brazil.
authors.forename: Thais F
authors.initials: TF
authors.lastname: Luciano
authors.affiliation: Faculty of Applied Sciences, University of Campinas (UNICAMP), 13083-872 Limeira, SP, Brazil ; Post Graduation Program in Motricity Science, Instituto de Biociências, São Paulo State University (UNESP), 13506-900 Rio Claro, SP, Brazil.
authors.forename: José R
authors.initials: JR
authors.lastname: Pauli
authors.affiliation: Post Graduation Program in Motricity Science, Instituto de Biociências, São Paulo State University (UNESP), 13506-900 Rio Claro, SP, Brazil.
authors.forename: Leandro P
authors.initials: LP
authors.lastname: Moura
authors.affiliation: Human Movement Laboratory, São Judas Tadeu University, 03166-000 São Paulo, SP, Brazil.
authors.forename: Erico
authors.initials: E
authors.lastname: Caperuto
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, Extremo Sul Catarinense University (UNESC), 88806-000 Criciuma, SC, Brazil.
authors.forename: Bruno L S
authors.initials: BL
authors.lastname: Pieri
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, Extremo Sul Catarinense University (UNESC), 88806-000 Criciuma, SC, Brazil.
authors.forename: Julia
authors.initials: J
authors.lastname: Engelmann
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, Extremo Sul Catarinense University (UNESC), 88806-000 Criciuma, SC, Brazil.
authors.forename: Gisele
authors.initials: G
authors.lastname: Scaini
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, Extremo Sul Catarinense University (UNESC), 88806-000 Criciuma, SC, Brazil.
authors.forename: Emilio L
authors.initials: EL
authors.lastname: Streck
authors.affiliation: Immunometabolism Research Group, Department of Physical Education, São Paulo State University (UNESP), 19060-900 Presidente Prudente, SP, Brazil.
authors.forename: Fabio S
authors.initials: FS
authors.lastname: Lira
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, Extremo Sul Catarinense University (UNESC), 88806-000 Criciuma, SC, Brazil.
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Pinho
authors.affiliation: Faculty of Applied Sciences, University of Campinas (UNICAMP), 13083-872 Limeira, SP, Brazil.
authors.forename: Eduardo R
authors.initials: ER
authors.lastname: Ropelle
authors.affiliation: School of Physical Education and Sport of Ribeirão Preto, São Paulo University (USP), 14040-907 Ribeirão Preto, SP, Brazil.
authors.forename: Adelino S R
authors.initials: AS
authors.lastname: Silva
authors.affiliation: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, Extremo Sul Catarinense University (UNESC), 88806-000 Criciuma, SC, Brazil.
authors.forename: Cláudio T
authors.initials: CT
authors.lastname: De Souza
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010766:Phosphorylation; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D056564:Sirtuin 1 / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; D014157:Transcription Factors; D055372:AMP-Activated Protein Kinases; C488368:Sirt1 protein, rat; D056564:Sirtuin 1