title: Engineered silybin nanoparticles educe efficient control in experimental diabetes.
abstract: Silybin, is one imminent therapeutic for drug induced hepatotoxicity, human prostate adenocarcinoma and other degenerative organ diseases. Recent evidences suggest that silybin influences gluconeogenesis pathways favorably and is beneficial in the treatment of type 1 and type 2 diabetes. The compound however is constrained due to solubility (0.4 mg/mL) and bioavailabilty limitations. Appropriate nanoparticle design for silybin in biocompatible polymers was thus proposed as a probable solution for therapeutic inadequacy. New surface engineered biopolymeric nanoparticles with high silybin encapsulation efficiency of 92.11% and zeta potential of +21 mV were designed. Both the pure compound and the nanoparticles were evaluated in vivo for the first time in experimental diabetic conditions. Animal health recovered substantially and the blood glucose levels came down to near normal values after 28 days treatment schedule with the engineered nanoparticles. Restoration from hyperglycemic damage condition was traced to serum insulin regeneration. Serum insulin recovered from the streptozotocin induced pancreatic damage levels of 0.17 ± 0.01 µg/lit to 0.57 ± 0.11 µg/lit after nanoparticle treatment. Significant reduction in glycated hemoglobin level, and restoration of liver glycogen content were some of the other interesting observations. Engineered silybin nanoparticle assisted recovery in diabetic conditions was reasoned due to improved silybin dissolution, passive transport in nanoscale, and restoration of antioxidant status.
authors.affiliation: Department of Chemical Technology, University of Calcutta, Kolkata, West Bengal, India.
authors.forename: Suvadra
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Chemical Technology, University of Calcutta, Kolkata, West Bengal, India; Faculty of Technology (Pharmaceutical) University Malaysia Pahang (UMP), Pahang, Malaysia.
authors.forename: Partha
authors.initials: P
authors.lastname: Roy
authors.affiliation: Department of Bio-Physics, Molecular Biology and Bioinformatics, University of Calcutta, Kolkata, West Bengal, India.
authors.forename: Rajat
authors.initials: R
authors.lastname: Pal
authors.affiliation: Department of Chemical Technology, University of Calcutta, Kolkata, West Bengal, India; Centre for Research in Nanoscience and Nanotechnology, University of Calcutta, Kolkata, West Bengal, India.
authors.forename: Runa Ghosh
authors.initials: RG
authors.lastname: Auddy
authors.affiliation: Department of Bio-Physics, Molecular Biology and Bioinformatics, University of Calcutta, Kolkata, West Bengal, India; Centre for Research in Nanoscience and Nanotechnology, University of Calcutta, Kolkata, West Bengal, India.
authors.forename: Abhay Sankar
authors.initials: AS
authors.lastname: Chakraborti
authors.affiliation: Department of Chemical Technology, University of Calcutta, Kolkata, West Bengal, India; Centre for Research in Nanoscience and Nanotechnology, University of Calcutta, Kolkata, West Bengal, India.
authors.forename: Arup
authors.initials: A
authors.lastname: Mukherjee
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000493:pharmacokinetics; D001786:Blood Glucose / Q000378:metabolism*; D048271:Chitosan / Q000008:administration & dosage; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D000077385:Silybin; D012838:Silymarin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D013311:Streptozocin
chemical_list: D001672:Biocompatible Materials; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D012838:Silymarin; D000077385:Silybin; D013311:Streptozocin; D048271:Chitosan