title: Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture.
abstract: The purpose of this study was to investigate the relationship between the osteoblastic cells behavior and biotribocorrosion phenomena on bioactive titanium (Ti). Ti substrates submitted to bioactive anodic oxidation and etching treatments were cultured up to 28 days with MG63 osteoblast-like cells. Important parameters of in vitro bone-like tissue formation were assessed. Although no major differences were observed between the surfaces topography (both rough) and wettability (both hydrophobic), a significant increase in cell attachment and differentiation was detected on the anodized substrates as product of favorable surface morphology and chemical composition. Alkaline phosphatase production has increased (≈20 nmol/min/mg of protein) on the anodized materials, while phosphate concentration has reached the double of the etched material and calcium production increased (over 20 µg/mL). The mechanical and biological stability of the anodic surfaces were also put to test through biotribocorrosion sliding solicitations, putting in evidence the resistance of the anodic layer and the cells capacity of regeneration after implant degradation. The Ti osteointegration abilities were also confirmed by the development of strong cell-biomaterial bonds at the interface, on both substrates. By combining the biological and mechanical results, the anodized Ti can be considered a viable option for dentistry.
authors.affiliation: Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017, Bobigny, Paris, France; University of Minho, CT2M, Centre for Mechanical and Materials Technologies, Campus de Azurém, 4800-058, Guimarães, Portugal.
authors.forename: H P
authors.initials: HP
authors.lastname: Felgueiras
authors.forename: L
authors.initials: L
authors.lastname: Castanheira
authors.forename: S
authors.initials: S
authors.lastname: Changotade
authors.forename: F
authors.initials: F
authors.lastname: Poirier
authors.forename: S
authors.initials: S
authors.lastname: Oughlis
authors.forename: M
authors.initials: M
authors.lastname: Henriques
authors.forename: C
authors.initials: C
authors.lastname: Chakar
authors.forename: N
authors.initials: N
authors.lastname: Naaman
authors.forename: R
authors.initials: R
authors.lastname: Younes
authors.forename: V
authors.initials: V
authors.lastname: Migonney
authors.forename: J P
authors.initials: JP
authors.lastname: Celis
authors.forename: P
authors.initials: P
authors.lastname: Ponthiaux
authors.forename: L A
authors.initials: LA
authors.lastname: Rocha
authors.forename: D
authors.initials: D
authors.lastname: Lutomski
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D001859:Bone Neoplasms / Q000473:pathology; D017610:Calcium Compounds / Q000494:pharmacology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D048430:Cell Shape; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003343:Corrosion; D003764:Dental Materials / Q000737:chemistry*; D004563:Electrochemistry; D017276:Friction; D005994:Glycerophosphates / Q000494:pharmacology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D016348:Osseointegration; D010006:Osteoblasts / Q000378:metabolism* / Q000648:ultrastructure; D012516:Osteosarcoma / Q000473:pathology; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D000085:Acetates; D017610:Calcium Compounds; D020099:Coated Materials, Biocompatible; D003764:Dental Materials; D005994:Glycerophosphates; D010087:Oxides; D010710:Phosphates; D010758:Phosphorus; D014025:Titanium; D000469:Alkaline Phosphatase; C031463:beta-glycerophosphoric acid; C120662:calcium acetate
keywords: anodization; biotribocorrosion; osteoblastic cells; titanium biomaterial; wearing