title: Antibacterial and antioxidant activities of derriobtusone A isolated from Lonchocarpus obtusus.
abstract: This study evaluated the effect of derriobtusone A, a flavonoid isolated from Lonchocarpus obtusus, on two important pathogenic bacteria, Staphylococcus aureus and Escherichia coli, as well as its antioxidant activity and toxicity. Planktonic growth assays were performed, and the inhibition of biofilm formation was evaluated. In addition, antioxidant activity was assessed by DPPH radical scavenging assay, ferrous ion chelating assay, ferric-reducing antioxidant power assay, and β -carotene bleaching assay. Toxicity was evaluated by the brine shrimp lethality test. Results showed that derriobtusone A completely inhibited the planktonic growth of S. aureus at 250 and 500  μ g/mL; however, it did not have the same activity on E. coli. Derriobtusone A reduced the biomass and colony-forming unit (cfu) of S. aureus biofilm at concentrations of 250 and 500  μ g/mL. In various concentrations, it reduced the biofilm biomass of E. coli, and, in all concentrations, it weakly reduced the cfu. Derriobtusone A showed highly efficient antioxidant ability in scavenging DPPH radical and inhibiting β -carotene oxidation. The compound showed no lethality to Artemia sp. nauplii. In conclusion, derriobtusone A may be an effective molecule against S. aureus and its biofilm, as well as a potential antioxidant compound with no toxicity.
authors.affiliation: Laboratory of Biologically Active Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60.440-970 Fortaleza, CE, Brazil ; Institute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal ; Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Federal University of Ceará, 62.042-280 Fortaleza, CE, Brazil.
authors.forename: Mayron Alves
authors.initials: MA
authors.lastname: Vasconcelos
authors.affiliation: Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Federal University of Ceará, 62.042-280 Fortaleza, CE, Brazil.
authors.forename: Francisco Vassiliepe Sousa
authors.initials: FV
authors.lastname: Arruda
authors.affiliation: Marine Natural Products Laboratory, Department of Fishing Engineering, Federal University of Ceará, 60.440-970 Fortaleza, CE, Brazil.
authors.forename: Daniel Barroso
authors.initials: DB
authors.lastname: de Alencar
authors.affiliation: Marine Natural Products Laboratory, Department of Fishing Engineering, Federal University of Ceará, 60.440-970 Fortaleza, CE, Brazil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Saker-Sampaio
authors.affiliation: Centre of Exact Sciences and Technology, Acaraú Valley State University, 62.040-370 Sobral, CE, Brazil.
authors.forename: Maria Rose Jane Ribeiro
authors.initials: MR
authors.lastname: Albuquerque
authors.affiliation: Centre of Exact Sciences and Technology, Acaraú Valley State University, 62.040-370 Sobral, CE, Brazil.
authors.forename: Hélcio Silva
authors.initials: HS
authors.lastname: Dos Santos
authors.affiliation: Centre of Exact Sciences and Technology, Acaraú Valley State University, 62.040-370 Sobral, CE, Brazil.
authors.forename: Paulo Nogueira
authors.initials: PN
authors.lastname: Bandeira
authors.affiliation: Department of Organic and Inorganic Chemistry, Federal University of Ceará, 60.021-940 Fortaleza, CE, Brazil.
authors.forename: Otília Deusdênia Loiola
authors.initials: OD
authors.lastname: Pessoa
authors.affiliation: Laboratory of Biologically Active Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60.440-970 Fortaleza, CE, Brazil.
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.affiliation: Institute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Mariana
authors.identifier: 0000-0003-0317-4877
authors.initials: M
authors.lastname: Henriques
authors.affiliation: Institute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Maria Olivia
authors.identifier: 0000-0002-4307-3985
authors.initials: MO
authors.lastname: Pereira
authors.affiliation: Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Federal University of Ceará, 62.042-280 Fortaleza, CE, Brazil.
authors.forename: Edson Holanda
authors.initials: EH
authors.lastname: Teixeira
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D018441:Biofilms / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity; D005419:Flavonoids / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; C000595382:derriobtusone A