title: Antimicrobial activity of human eosinophil granule proteins.
abstract: Eosinophils secrete a number of proinflammatory mediators, like cytokines, chemokines, and granule proteins which are responsible for the initiation and sustenance of inflammatory response caused by them. The eosinophil granule proteins, ECP, EDN, MBP, and EPO possess antimicrobial activity against bacteria, helminths, protozoa, and viruses. In this chapter, we describe various assays used to detect and quantitate the antimicrobial activities of eosinophil granule proteins, particularly ECP and EDN. We have taken a model organism for each assay and described the method for antiviral, antihelminthic, antiprotozoan, and antibacterial activity of purified eosinophil granule proteins.
authors.affiliation: Immunochemistry Laboratory, National Institute of Immunology, New Delhi, India.
authors.forename: Anu
authors.initials: A
authors.lastname: Chopra
authors.forename: Janendra K
authors.initials: JK
authors.lastname: Batra
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D047091:Eosinophil Granule Proteins / Q000494:pharmacology*; D006376:Helminths / Q000187:drug effects; D006801:Humans; D014780:Viruses / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D047091:Eosinophil Granule Proteins