title: Synthesis of poly(N-isopropylacrylamide)-co-poly(phenylboronate ester) acrylate and study on their glucose-responsive behavior.
abstract: We introduced thermo-sensitive poly(N-isopropylacrylamide) (PNIPAM) into the polymer structure of poly(ethylene glycol)-block-poly(phenylboronate ester) acrylate (MPEG-block-PPBDEMA) by block and random polymerization pathways in order to investigate the effect of polymer architecture on the glucose-responsiveness and enhance their insulin release controllability. By following the structure, the continuous PNIPAM shell of the triblock polymer MPEG-block-PNIPAM-block-PPBDEMA collapsing on the glucose-responsive PPBDEMA core formed the polymeric micelles with a core-shell-corona structure, and MPEG-block-(PNIPAM-rand-PPBDEMA) exhibited core-corona micelles in which the hydrophobic core consisted of PNIPAM and PPBDEMA segments when the environmental temperature was increased above low critical solution temperature (LCST) of PNIPAM. The micellar morphologies can be precisely controlled by temperature change between 15 and 37°C. As a result, the introduction of PNIPAM greatly enhanced the overall stability of insulin encapsulated in the polymeric micelles in the absence of glucose over incubation 80 h at 37°C. Comparing to MPEG-block-PNIPAM-block-PPBDEMA, the nanocarriers from MPEG-block-(PNIPAM-rand-PPBDEMA) showed great insulin release behavior which is zero insulin release without glucose, low release at normal blood glucose concentration (1.0 mg/mL). Therefore, these nanocarriers may be served as promising self-regulated insulin delivery system for diabetes treatment.
authors.affiliation: State Key Laboratory of Chemical Resource, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Chemical Resource, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Heyun
authors.initials: H
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Chemical Resource, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Chemical Resource, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: yangj@mail.buct.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Anesthesia and Pain Therapy, Beijing Tiantan Hospital Affiliated to Capital Medical University, Beijing 100050, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Jin
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry*; D008823:Micelles; D011091:Polyesters / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D007328:Insulin; D008823:Micelles; D011091:Polyesters; C052970:poly-N-isopropylacrylamide; D005947:Glucose
keywords: Glucose-responsive; Insulin release; PNIPAM; Random polymerization; Triblock polymer