title: The role of duplex stability for wavelength-shifting fluorescent DNA probes: energy transfer vs. exciton interactions in DNA "traffic lights".
abstract: Exciton interactions between thiazole orange and thiazole red as nucleotide substitutes in DNA hairpins interfere with efficient energy transfer and fluorescence color change as readout. This interference can be tuned by two structural parameters that control the hairpin duplex stability.
authors.affiliation: Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131 Karlsruhe, Germany. Wagenknecht@kit.edu.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Barrois
authors.forename: Samantha
authors.initials: S
authors.lastname: Wörner
authors.forename: Hans-Achim
authors.initials: HA
authors.lastname: Wagenknecht
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D002232:Carbocyanines / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry* / Q000528:radiation effects; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D055668:Photochemical Processes; D011804:Quinolines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
chemical_list: D052160:Benzothiazoles; D002232:Carbocyanines; D015342:DNA Probes; D005456:Fluorescent Dyes; D011804:Quinolines; C584189:thiazole red; C051000:thiazole orange