title: Inhibition of TRPM7 channels reduces degranulation and release of cytokines in rat bone marrow-derived mast cells.
abstract: BACKGROUND
  mast cells play an important role in airway inflammation in asthma. The transient receptor potential melastatin-like 7 (TRPM7) channel is expressed in primary human lung mast cells and plays a critical role for cell survival. This study aimed to investigate the role of TRPM7 on degranulation and release of cytokines in rat bone marrow-derived mast cells (BMMCs).
  
  
  METHODS
  the expression levels of TRPM7 were observed by immunocytochemistry and RT-PCR between normal and asthmatic rat BMMCs. TRPM7-specific shRNA and 2-aminoethoxydiphenyl borate (2-APB) and specific shTRPM7 were used to inhibit the function of TRPM7. Degranulation levels were analyzed by beta-hexosaminidase assay. Histamine, TNF-α, IL-6 and IL-13 levels were measured by ELISA.
  
  
  RESULTS
  the expression of TRPM7 was significantly higher in asthmatic rat BMMCs than in the normal control group. After application of 2-APB and down-regulation of TRPM7, the beta-hexosaminidase activity and secretion of histamine, IL-6, IL-13 and TNF-α were significantly decreased in the asthmatic group compared to the control group.
  
  
  CONCLUSIONS
  this study indicates that TRPM7 channels may be involved in the process of degranulation and release of cytokines in rat bone marrow-derived mast cells.
authors.affiliation: Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Institute for Respiratory Disease of Sun Yat-sen University, Sun Yat-sen University, Guangzhou 510120, China. hlinj@mail.sysu.edu.cn.
authors.forename: Linjie
authors.initials: L
authors.lastname: Huang
authors.affiliation: Clinical Department of School of Medicine, Shenzhen University, Shenzhen 518060, China. wuyimei@szu.edu.cn.
authors.forename: Ngai-Mui
authors.initials: NM
authors.lastname: Ng
authors.affiliation: Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Institute for Respiratory Disease of Sun Yat-sen University, Sun Yat-sen University, Guangzhou 510120, China. chenmingsysu@gmail.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Institute for Respiratory Disease of Sun Yat-sen University, Sun Yat-sen University, Guangzhou 510120, China. xiaolinglinsysu@gmail.com.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Institute for Respiratory Disease of Sun Yat-sen University, Sun Yat-sen University, Guangzhou 510120, China. tiantiantangsysu@gmail.com.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Tang
authors.affiliation: Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Institute for Respiratory Disease of Sun Yat-sen University, Sun Yat-sen University, Guangzhou 510120, China. huihuachengsysu@gmail.com.
authors.forename: Huihua
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Respiratory Medicine, Mei Zhou People's Hospital, Meizhou Affiliated Hospital of Sun Yat-sen University, Meizhou 514031, China. yangchengsysu@gmail.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Institute for Respiratory Disease of Sun Yat-sen University, Sun Yat-sen University, Guangzhou 510120, China. shanpingjiang@126.com.
authors.forename: Shanping
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001249:Asthma / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism*; D001896:Boron Compounds / Q000494:pharmacology; D015550:Cell Degranulation; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006632:Histamine / Q000378:metabolism; D008407:Mast Cells / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D050053:TRPM Cation Channels / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D001896:Boron Compounds; D016207:Cytokines; D050053:TRPM Cation Channels; D006632:Histamine; C109986:2-aminoethoxydiphenyl borate; C486584:Trpm7 protein, rat