title: Phytopathogenic bacteria phenotype conversion as a result of their lysogenisation by coliphage P1.
abstract: A set of lysogenic strains of phytopathogenic bacteria Erwinia "horticola" and Erwinia amylovora associated with woody plants was obtained using bacteriophage P1 Cmc1ts100. The phenotype conversion from Cm(S) to Cm(R) was shown to be connected with introducing of authentic prophage DNA of 94.8 kb as a single-copy plasmid into the cells. Prophage state is unstable: P1 plasmid is spontaneously lost with high frequency by the cells. In lysogenic cells the prophage genes of type III restriction-modification complex EcoP1I are actively expressed. The system formed by E. "horticola" 450 and 60 as well as their lysogenic derivatives and specific bacteriophages provides an opportunity to divide the latter into three groups according to the level of restriction in the course of their interaction with the enzyme EcoP1I. The difference in phage responses to the endonuclease presence in a lysogenized host presumably correlates with the number of enzyme recognition sequences and the adsorption sites availability. After the prophage plasmid DNA curing the characteristic value of phage sensitivity of cells is changed. The lysogenic strains obtained in this work allow for the exploration of EcoP1I restriction-modification gene complex interaction with polyvalent phages able to grow not only on E. coli, but also on such phytopathogens as E. "horticola" and E. amylovora.
authors.forename: I V
authors.initials: IV
authors.lastname: Faidiuk
authors.forename: E I
authors.initials: EI
authors.lastname: Tovkach
mesh_terms: D017101:Bacteriophage P1 / Q000235:genetics*; D004279:DNA, Viral; D015263:Deoxyribonucleases, Type III Site-Specific / Q000235:genetics / Q000378:metabolism; D004885:Erwinia / Q000821:virology*; D044084:Erwinia amylovora / Q000821:virology*; D005814:Genes, Viral; D005838:Genotype; D008242:Lysogeny / Q000235:genetics*; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D056265:Microbial Interactions / Q000235:genetics*; D010641:Phenotype; D010944:Plants / Q000382:microbiology; D010957:Plasmids; D039002:Prophages / Q000235:genetics
chemical_list: D004279:DNA, Viral; D008780:Methyltransferases; C030432:endodeoxyribonuclease EcoP1; D015263:Deoxyribonucleases, Type III Site-Specific