title: Regulatory B cells in allergic airways disease and asthma.
abstract: B cells have long been known to participate in both innate and adaptive immune responses by contributing to antigen presentation and by producing antigen-specific antibodies. Recent evidence shows that certain B-cell subsets can also inhibit T-cell immune responses. Like regulatory T cells (Treg), these regulatory B cells (Breg) appear to comprise several subpopulations. How Breg cells are generated and how they control immune responses in vivo are just beginning to be elucidated. Here, we provide detailed instructions for the identification, isolation, and functional characterization of Breg cells in a murine model of allergic airway disease.
authors.affiliation: Department of Immunology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA.
authors.forename: Prabitha
authors.initials: P
authors.lastname: Natarajan
authors.forename: Linda A
authors.initials: LA
authors.lastname: Guernsey
authors.forename: Craig M
authors.initials: CM
authors.lastname: Schramm
mesh_terms: D000818:Animals; D060151:B-Lymphocytes, Regulatory / Q000276:immunology* / Q000473:pathology*; D018893:Bronchoalveolar Lavage / Q000379:methods; D002469:Cell Separation / Q000379:methods; D016616:Cryoultramicrotomy / Q000379:methods; D004195:Disease Models, Animal; D051379:Mice / Q000276:immunology*; D018613:Microscopy, Confocal / Q000379:methods; D012130:Respiratory Hypersensitivity / Q000097:blood / Q000276:immunology* / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology