title: Metal mesh device sensor immobilized with a trimethoxysilane-containing glycopolymer for label-free detection of proteins and bacteria.
abstract: Biosensors for the detection of proteins and bacteria have been developed using glycopolymer-immobilized metal mesh devices. The trimethoxysilane-containing glycopolymer was immobilized onto a metal mesh device using the silane coupling reaction. The surface shape and transmittance properties of the original metal mesh device were maintained following the immobilization of the glycopolymer. The mannose-binding protein (concanavalin A) could be detected at concentrations in the range of 10(-9) to 10(-6) mol L(-1) using the glycopolymer-immobilized metal mesh device sensor, whereas another protein (bovine serum albumin) was not detected. A detection limit of 1 ng mm(-2) was achieved for the amount of adsorbed concanavalin A. The glycopolymer-immobilized metal mesh device sensor could also detect bacteria as well as protein. The mannose-binding strain of Escherichia coli was specifically detected by the glycopolymer-immobilized metal mesh device sensor. The glycopolymer-immobilized metal mesh device could therefore be used as a label-free biosensor showing high levels of selectivity and sensitivity toward proteins and bacteria.
authors.affiliation: Graduate School of Engineering, Kyushu University , 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Seto
authors.forename: Seiji
authors.initials: S
authors.lastname: Kamba
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Makoto
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Shigeki
authors.initials: S
authors.lastname: Nashima
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ehara
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Yu
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Miura
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001422:Bacterial Adhesion; D015374:Biosensing Techniques / Q000295:instrumentation*; D003198:Computer Simulation; D003208:Concanavalin A / Q000302:isolation & purification; D004574:Electromagnetic Fields; D004926:Escherichia coli / Q000302:isolation & purification; D008362:Mannosides / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D012821:Silanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling; D013499:Surface Properties
chemical_list: D008362:Mannosides; D008670:Metals; D011506:Proteins; D012821:Silanes; D003208:Concanavalin A; D012710:Serum Albumin, Bovine; C071717:trimethoxysilane