title: An electrochemical impedimetric aptasensing platform for sensitive and selective detection of small molecules such as chloramphenicol.
abstract: We report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partially blocked due to the formation of the aptamer-CAP complex, resulting in an increase of the interfacial electron-transfer resistance of the redox probe detected by electrochemical impedance spectroscopy or cyclic voltammetry. We observed that the ratio of polarization resistance had a linear relationship with the concentrations of CAP in the range of 1.76-127 nM, and a detection limit of 1.76 nM was obtained. The covalent binding of CAP-aptamer on the electrode surface combined with the unique properties of aptamers and impedimetric transduction leads to the development of a stable and sensitive electrochemical aptasensor for CAP.
authors.affiliation: AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. sanaz.pilehvar@uantwerpen.be.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Pilehvar
authors.affiliation: Applied Engineering and Technology-Chemistry, University of Antwerp, Salesianenlaan 30, 2000 Antwerp, Belgium. tarryn.dierckx@gmail.com.
authors.forename: Tarryn
authors.initials: T
authors.lastname: Dierckx
authors.affiliation: Sphere Research Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. ronny.blust@uantwerpen.be.
authors.forename: Ronny
authors.initials: R
authors.lastname: Blust
authors.affiliation: Applied Engineering and Technology-Chemistry, University of Antwerp, Salesianenlaan 30, 2000 Antwerp, Belgium. tom.breugelmans@uantwerpen.be.
authors.forename: Tom
authors.initials: T
authors.lastname: Breugelmans
authors.affiliation: AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Karolien.dewael@uantwerpen.be.
authors.forename: Karolien
authors.initials: K
authors.lastname: De Wael
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002701:Chloramphenicol / Q000032:analysis* / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D008970:Molecular Weight; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D002701:Chloramphenicol