title: Neuroprotective effects of Z-ajoene, an organosulfur compound derived from oil-macerated garlic, in the gerbil hippocampal CA1 region after transient forebrain ischemia.
abstract: The neuroprotective effects of two isomers (Z- and E-) of ajoene, a major compound in oil-macerated garlic products, against ischemic damage were investigated in the gerbil hippocampus. Vehicle (corn oil), Z- or E-ajoenes (25 mg/kg) was orally administered 30 min prior to the induction of transient forebrain ischemia by occlusion of the common carotid arteries for 5 min. One day after ischemia/reperfusion (I/R), I/R-induced hyperactivity significantly reduced in the E- and Z-ajoene-treated groups, compared to that in the vehicle-treated group 5 days after I/R, the number of cresyl violet-positive neurons in the E- and Z-ajoene-treated groups increased, compared to that in the vehicle-treated group. Reactive gliosis in the CA1 region of E- and Z-ajoene-treated groups reduced, compared to that in the vehicle-treated group. These neuroprotective effects were more prominent in animals treated with Z-ajoene, than in those treated with E-ajoene. In addition, Z-ajoene significantly decreased lipid peroxidation, as indicated by 4-hydroxy-2-nonenal levels in hippocampal homogenates, compared to that observed in the vehicle-treated group at a range of time points after I/R. These results suggested that Z-ajoene protected against I/R-induced delayed neuronal death and gliosis by reducing lipid peroxidation in the gerbil hippocampal CA1 region.
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Yoo
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Woosuk
authors.initials: W
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Nam
authors.affiliation: Food Analysis Center, Korea Food Research Institute, Sungnam 463-746, South Korea.
authors.forename: Miyoung
authors.initials: M
authors.lastname: Yoo
authors.affiliation: Food Analysis Center, Korea Food Research Institute, Sungnam 463-746, South Korea.
authors.forename: Sanghee
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Yeo Sung
authors.initials: YS
authors.lastname: Yoon
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea. Electronic address: vetmed2@snu.ac.kr.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.affiliation: Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, South Korea. Electronic address: jhchoi@kangwon.ac.kr.
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D004220:Disulfides / Q000737:chemistry / Q000494:pharmacology*; D005737:Garlic / Q000737:chemistry*; D005849:Gerbillinae; D007150:Immunohistochemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000737:chemistry*; D016548:Prosencephalon / Q000187:drug effects / Q000473:pathology; D013454:Sulfoxides
chemical_list: D000447:Aldehydes; D004220:Disulfides; D018696:Neuroprotective Agents; D010938:Plant Oils; D013454:Sulfoxides; C048980:ajoene; C027576:4-hydroxy-2-nonenal
keywords: Ajoene; Lipid peroxidation; Neuroprotection; Reactive gliosis; Spontaneous motor activity; Transient forebrain ischemia