title: Sequence analysis and feeding responses evoked by the large molecular form of gastrin releasing peptide (GRP) in the rat GRP-29.
abstract: Microisolation techniques utilizing several reverse phase high performance liquid chromatography (HPLC) steps have resulted in the purification of two rat gastrin releasing peptide (GRP) forms suitable for microsequence and mass spectral analysis. The sequence of the larger form is APVSTGAGGGTVLAKMYPRGSHWAVGHLM-amide and the smaller form is GSHWAVGHLM-amide which is the carboxyl terminal decapeptide of the larger peptide. The peptides were synthesized and their feeding patterns e.g. first meal size (MS), intermeal interval (IMI) and satiety ratio (SR, IMI/MS) were determined in overnight food-, but not water deprived, male Sprague Dawley rats. The peptides were administered in the femoral vein (0, 0.21, 0.41 and 1.03 nmol/kg) immediately before presenting the rats with a 10% sucrose solution. We found that (1) GRP-10 (all doses) and GRP-29 (0.41 nmol/kg) reduced first MS, (2) both peptides prolonged IMI length and (3) both peptides increased the SR to similar extents. In conclusion, GRP-10 and GRP-29 are the two endogenous forms of GRP in the rat intestine and they reduce short term feeding to similar extents when administered intravenously.
authors.affiliation: CURE: Digestive Diseases Research Center VA GLAHS, Los Angeles, CA, USA; Digestive Diseases Division, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Reeve
authors.affiliation: Gastroenterology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL, USA.
authors.forename: Martha C
authors.initials: MC
authors.lastname: Washington
authors.affiliation: Gastroenterology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL, USA.
authors.forename: Karen H
authors.initials: KH
authors.lastname: Park
authors.affiliation: Gastroenterology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL, USA.
authors.forename: Tanisha
authors.initials: T
authors.lastname: Johnson
authors.affiliation: Gastroenterology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL, USA.
authors.forename: Jizette
authors.initials: J
authors.lastname: Hunt
authors.affiliation: Division of Immunology, Beckman Research Institute of City of Hope, Duarte, CA, USA.
authors.forename: John E
authors.initials: JE
authors.lastname: Shively
authors.affiliation: Division of Immunology, Beckman Research Institute of City of Hope, Duarte, CA, USA.
authors.forename: Mike
authors.initials: M
authors.lastname: Ronk
authors.affiliation: Division of Immunology, Beckman Research Institute of City of Hope, Duarte, CA, USA.
authors.forename: Terry D
authors.initials: TD
authors.lastname: Lee
authors.affiliation: Department of Physiology, Tokushima Bunri University, Tokushima, Japan.
authors.forename: Yoshi
authors.initials: Y
authors.lastname: Goto
authors.affiliation: CURE: Digestive Diseases Research Center VA GLAHS, Los Angeles, CA, USA; Digestive Diseases Division, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Chew
authors.affiliation: CURE: Digestive Diseases Research Center VA GLAHS, Los Angeles, CA, USA; Digestive Diseases Division, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
authors.forename: Fang-Jen
authors.initials: FJ
authors.lastname: Ho
authors.affiliation: Gastroenterology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL, USA. Electronic address: sayeghai@mytu.tuskegee.edu.
authors.forename: Ayman I
authors.initials: AI
authors.lastname: Sayegh
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005247:Feeding Behavior / Q000187:drug effects*; D019886:Gastrin-Releasing Peptide / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D040081:Protein Array Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019886:Gastrin-Releasing Peptide
keywords: Gastrin releasing peptide-29; Intermeal interval; Intravenous; Meal size; Rat