title: Diamond nanowires modified with poly[3-(pyrrolyl)carboxylic acid] for the immobilization of histidine-tagged peptides.
abstract: Coating boron-doped diamond nanowires (BDD NWs) with a conducting polymer, poly[3-(pyrrolyl)carboxylic acid], has been reported. Polymer coating was achieved through electropolymerization of 3-(pyrrolyl)carboxylic acid at the electrode interface by amperometrically biasing the BDD NWs interface until a predefined charge has passed. The poly[3-(pyrrolyl)carboxylic acid] modified BDD NWs (PPA-BDD NWs) were characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV). Using a deposition charge of 11 mC cm(-2) resulted in a thin polymer film deposition. The availability of the carboxylic groups of the polymer coated BDD NWs electrode was demonstrated through copper ion (Cu(2+)) chelation. The resulting complex was successfully used for the site-specific immobilization of histidine-tagged peptides. The binding process was followed by electrochemical impedance spectroscopy (EIS). The Cu(2+)-chelated PPA-BDD NWs interface showed peptide loading capability comparable to those of commercially available interfaces and can be easily regenerated several times using ethylenediaminetetraacetic acid (EDTA).
authors.affiliation: Institut de Recherche Interdisciplinaire (IRI), CNRS USR 3078, Université Lille1, Parc de la Haute Borne, 50 avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France. Sabine.Szunerits@iri.univ-lille1.fr.
authors.forename: Palaniappan
authors.initials: P
authors.lastname: Subramanian
authors.forename: Ievgen
authors.initials: I
authors.lastname: Mazurenko
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zaitsev
authors.forename: Yannick
authors.initials: Y
authors.lastname: Coffinier
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D002264:Carboxylic Acids / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D018130:Diamond / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D006639:Histidine / Q000032:analysis*; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D009842:Oligopeptides / Q000032:analysis*; D010455:Peptides / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D002264:Carboxylic Acids; D002614:Chelating Agents; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D010455:Peptides; D011758:Pyrroles; C569491:poly(3-(pyrrolyl)carboxylic acid); D006639:Histidine; D018130:Diamond; D003300:Copper