title: Identification of speckle-type POZ protein somatic mutations in African American prostate cancer.
abstract: The speckle-type POZ protein (SPOP) is a tumor suppressor in prostate cancer (PCa). SPOP somatic mutations have been reported in up to 15% of PCa of those of European descent. However, the genetic roles of SPOP in African American (AA)-PCa are currently unknown. We sequenced the SPOP gene to identify somatic mutations in 49 AA prostate tumors and identified three missense mutations (p.Y87C, p.F102S, and p.G111E) in five AA prostate tumors (10%) and one synonymous variant (p.I106I) in one tumor. Intriguingly, all of mutations and variants clustered in exon six, and all of the mutations altered conserved amino acids. Moreover, two mutations (p.F102S and p.G111E) have only been identified in AA-PCa to date. Quantitative real-time polymerase chain reaction analysis showed a lower level of SPOP expression in tumors carrying SPOP mutations than their matched normal prostate tissues. In addition, SPOP mutations and novel variants were detected in 5 of 27 aggressive PCa and one of 22 less aggressive PCa (P < 0.05). Further studies with increased sample size are needed to validate the clinicopathological significance of these SPOP mutations in AA-PCa.
authors.forename: Eric
authors.initials: E
authors.lastname: Buckles
authors.forename: Chiping
authors.initials: C
authors.lastname: Qian
authors.forename: Andrew
authors.initials: A
authors.lastname: Tadros
authors.forename: Sumana
authors.initials: S
authors.lastname: Majumdar
authors.forename: Jennifer
authors.initials: J
authors.lastname: Cvitanovic
authors.forename: Jovanny
authors.initials: J
authors.lastname: Zabaleta
authors.forename: John
authors.initials: J
authors.lastname: Estrada
authors.forename: John
authors.initials: J
authors.lastname: Wilson
authors.affiliation: Department of Genetics; Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA.
authors.forename: Wanguo
authors.initials: W
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D044383:Black People; D017931:DNA Primers; D006801:Humans; D008297:Male; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D001741:Black or African American
chemical_list: D017931:DNA Primers; D009687:Nuclear Proteins; D012097:Repressor Proteins; C509761:SPOP protein, human