title: Polymeric nanoparticles-embedded organogel for roxithromycin delivery to hair follicles.
abstract: Drug delivery into hair follicles with the use of nanoparticles (NPs) is gaining more importance as drug-loaded NPs may accumulate in hair follicle openings. The aim was to develop and evaluate a pluronic lecithin organogel (PLO) with roxithromycin (ROX)-loaded NPs for follicular targeting. Polymeric NPs were evaluated in terms of particle shape, size, zeta potential, suspension stability, encapsulation efficiency and in vitro drug release. Lyophilized NPs were incorporated into the PLO and rheological measurements of the nanoparticles-embedded organogels were done. The fate of the NPs in the skin was traced by incorporation of a fluorescent dye into the NPs. As a result, ROX was efficiently incorporated into polymeric NPs characterized by the appropriate size (approximately 300 nm) allowing drug delivery to hair follicles. In ex vivo human skin penetration studies, horizontal skin sections revealed fluorescence deep in the hair follicles. Although the organogel has higher affinity to the lipidic follicular area than an aqueous suspension of NPs, it did not seem to improve penetration of the NPs along the hair shaft. The results proved that it was possible to achieve preferential targeting to the pilosebaceous unit using polymeric NPs formulated either into the aqueous suspension or semisolid topical formulation.
authors.affiliation: Department of Pharmaceutical Technology, Poznan University of Medical Sciences, Poznań, Poland. Electronic address: eglowka@ump.edu.pl.
authors.forename: Eliza
authors.initials: E
authors.lastname: Główka
authors.affiliation: Department of Pharmaceutical Technology, Poznan University of Medical Sciences, Poznań, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Wosicka-Frąckowiak
authors.affiliation: Department of Pharmaceutical Technology, Poznan University of Medical Sciences, Poznań, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Hyla
authors.affiliation: Department of Pharmaceutical Technology, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Stefanowska
authors.affiliation: NanoBioMedical Centre, Adam Mickiewicz University, Poznań, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jastrzębska
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Klapiszewski
authors.affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland.
authors.forename: Teofil
authors.initials: T
authors.lastname: Jesionowski
authors.affiliation: Department of Pharmaceutical Technology, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Cal
mesh_terms: D000287:Administration, Topical; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D005453:Fluorescence; D005612:Freeze Drying; D005782:Gels / Q000737:chemistry; D018859:Hair Follicle / Q000187:drug effects*; D006801:Humans; D054709:Lecithins / Q000008:administration & dosage*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry; D010316:Particle Size; D020442:Poloxamer / Q000008:administration & dosage*; D011108:Polymers / Q000737:chemistry*; D012212:Rheology; D015575:Roxithromycin / Q000008:administration & dosage*; D033081:Shear Strength; D012867:Skin / Q000187:drug effects*; D055672:Static Electricity
chemical_list: D004337:Drug Carriers; D005782:Gels; D054709:Lecithins; D010078:Oxazines; D011108:Polymers; D020442:Poloxamer; D015575:Roxithromycin; C044808:nile red
keywords: Apparatus 4 dialysis adapter; Hair follicle targeting; Nanoparticles; Pluronic lecithin organogel; Poly(epsilon-caprolactone); Roxithromycin