title: TLR9 and NF-κB are partially involved in activation of human neutrophils by Helicobacter pylori and its purified DNA.
abstract: Helicobacter pylori infection represents one of the most common bacterial infections worldwide. The inflammatory response to this bacterium involves a large influx of neutrophils to the lamina propria of the gastric mucosa. However, little is known about the receptors and molecular mechanisms involved in activation of these neutrophils. In this study, we aimed to determine the role of toll-like receptor 9 (TLR9) in the response of human neutrophils to H. pylori and purified H. pylori DNA (Hp-DNA). Neutrophils were isolated from the blood of adult volunteers and challenged with either H. pylori or Hp-DNA. We found that both, H. pylori and Hp-DNA induced increased expression and release of IL-8. Furthermore, we showed that TLR9 is involved in the induction of IL-8 production by H. pylori and Hp-DNA. IL-8 production induced by H. pylori but not by Hp-DNA was partially mediated by NF-κB. In conclusion, this study showed for first time that both, H. pylori and Hp-DNA activate TLR9 and induce a different inflammatory response that leads to activation of neutrophils.
authors.affiliation: Unidad de Investigación en Enfermedades Infecciosas, Hospital de Pediatría del Instituto Mexicano del Seguro Social, Mexico City, México; Laboratorio de Neuroinmunobiología del Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología de la Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Alvarez-Arellano
authors.affiliation: Departamento de Biología Celular del Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional, Mexico City, México.
authors.forename: Pedro
authors.initials: P
authors.lastname: Cortés-Reynosa
authors.affiliation: Unidad de Investigación en Enfermedades Infecciosas, Hospital de Pediatría del Instituto Mexicano del Seguro Social, Mexico City, México; Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, Mexico City, México.
authors.forename: Norma
authors.initials: N
authors.lastname: Sánchez-Zauco
authors.affiliation: Departamento de Biología Celular del Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional, Mexico City, México.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Salazar
authors.affiliation: Unidad de Investigación en Enfermedades Infecciosas, Hospital de Pediatría del Instituto Mexicano del Seguro Social, Mexico City, México.
authors.forename: Javier
authors.initials: J
authors.lastname: Torres
authors.affiliation: Unidad de Investigación en Enfermedades Infecciosas, Hospital de Pediatría del Instituto Mexicano del Seguro Social, Mexico City, México; Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, Mexico City, México.
authors.forename: Carmen
authors.initials: C
authors.lastname: Maldonado-Bernal
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000276:immunology*; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D016328:NF-kappa B / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology*; D051217:Toll-Like Receptor 9 / Q000276:immunology*
chemical_list: D004269:DNA, Bacterial; D016328:NF-kappa B; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9