title: Automated derivatization and fluorimetric determination of biogenic amines in milk by zone fluidics coupled to liquid chromatography.
abstract: A novel zone-fluidics/high pressure liquid chromatographic (ZF-HPLC) method is described for the simultaneous determination of six biogenic monoamines, in the presence of hexylamine as internal standard. Automated on-line derivatization of the analytes with naphthalene-2,3-dicarboxaldehyde/cyanide ions was performed using the ZF concept and the derivatives were injected on the HPLC column for separation/detection. The influence of the ZF operation conditions on the derivatization reaction was investigated. The isoindoles formed were separated on a Kromasil C18 column (250 mm × 4 mm i.d., 5 μm), using an isocratic mobile phase of methanol/water (80:20, v/v) at a flow rate of 1.0 mL min(-1). Monitoring and quantification was carried out by fluorescence detection at 424/494 nm. The limits of detection were at the pg level with a sample volume of 20 μL. The whole procedure was evaluated and fully validated for the determination of biogenic amines in milk samples.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Notou
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece. Electronic address: azotou@chem.auth.gr.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Zotou
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
authors.forename: Paraskevas D
authors.initials: PD
authors.lastname: Tzanavaras
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
authors.forename: Demetrius G
authors.initials: DG
authors.lastname: Themelis
mesh_terms: D000818:Animals; D001322:Autoanalysis; D001679:Biogenic Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D005470:Fluorometry; D008892:Milk / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry; D012015:Reference Standards
chemical_list: D001679:Biogenic Amines; D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde
keywords: Biogenic amines; Liquid chromatography; Milk; Naphthalene-2,3-dicarboxaldehyde; Sequential injection analysis