title: Blocked autophagy by miR-101 enhances osteosarcoma cell chemosensitivity in vitro.
abstract: The adjuvant chemotherapy, such as cisplatin, doxorubicin, and methotrexate has significantly improved survival of osteosarcoma patients. However, the chemoresistance which arose with the chemotherapy blocks achieving favorable outcomes for some patients and finally led to relapse or metastatic disease. Studies have shown paradoxical functions of autophagy in tumor development, which has been demonstrated by microRNAs. In the present study, we determined the involvement of autophagy during the chemotherapy of osteosarcoma cell line, U-2 OS, and further determined the regulation of miR-101 on the autophagy in the U-2 OS cells. Results demonstrated that doxorubicin treatment of U-2 OS cells induced significantly high level of autophagy-characteristic acidic vesicular organelles (AVOs), and induced significant autophagy related protein expression in U-2 OS cells. While the miR-101 could significantly reduce the doxorubicin-induced AVOs and block the autophagy related protein expression in U-2 OS cells. Moreover, the autophagy blockage by miR-101 sensitized the U-2 OS cells to doxorubicin treatment. In summary, miR-101 blocks autophagy during the chemotherapy in osteosarcoma cells and enhances chemosensitivity in vitro.
authors.affiliation: The Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing 400016, China ; The Department of Cervical Surgery, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Chang
authors.affiliation: The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Huo
authors.affiliation: The Department of Anesthesiology, The PLA 264 Hospital, Taiyuan 030001, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.affiliation: The Department of Cervical Surgery, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wu
authors.affiliation: The Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing 400016, China.
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001343:Autophagy; D022162:Cytoplasmic Vesicles / Q000187:drug effects / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D004317:Doxorubicin