title: Controlled glycosylation of plant-produced recombinant proteins.
abstract: Despite their recognized importance for therapeutic proteins, the production of structurally defined glycans is still a challenging issue. However, an increased understanding of glycosylation pathways, recent advances in analytical tools, and emerging technologies for subcellular targeting using chimeric glycosyltransferases are facilitating the rational design of new glycan biosynthetic pathways. Plants are particularly amenable to glyco-engineering approaches and thus they are increasingly being used for the production of recombinant proteins. Here we summarize the main achievements in the field of in planta glyco-engineering for the production of therapeutically relevant proteins.
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences (BOKU), Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Richard
authors.initials: R
authors.lastname: Strasser
authors.affiliation: Department of Chemistry, University of Natural Resources and Life Sciences (BOKU), Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Altmann
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences (BOKU), Muthgasse 18, A-1190 Vienna, Austria. Electronic address: herta.steinkellner@boku.ac.at.
authors.forename: Herta
authors.initials: H
authors.lastname: Steinkellner
mesh_terms: D000818:Animals; D006031:Glycosylation; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D008322:Mammals / Q000235:genetics; D060847:Metabolic Engineering; D059828:Plant Cells / Q000378:metabolism; D010944:Plants / Q000235:genetics* / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D011134:Polysaccharides; D011994:Recombinant Proteins; D016695:Glycosyltransferases