title: Analysis of small RNAs derived from Chinese wheat mosaic virus.
abstract: The virus-derived small interfering RNAs (vsiRNAs) of Chinese wheat mosaic virus (CWMV), a member of the genus Furovirus, were characterised from wheat plants by deep sequencing. CWMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CWMV-infected wheat plants. The 5'-terminal base of vsiRNAs was biased towards A/U, suggesting that CWMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were predicted.
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Shi-Ling
authors.initials: SL
authors.lastname: Zheng
authors.forename: Heng-Mu
authors.initials: HM
authors.lastname: Zhang
authors.forename: Xiao-Ya
authors.initials: XY
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jun-Min
authors.initials: JM
authors.lastname: Li
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Chen
mesh_terms: D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D034741:RNA, Small Interfering / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D017423:Sequence Analysis, RNA; D014908:Triticum / Q000235:genetics / Q000821:virology*
chemical_list: D034741:RNA, Small Interfering; D012367:RNA, Viral