title: Novel polymeric ionic liquid microspheres with high exchange capacity for fast extraction of plasmid DNA.
abstract: A novel polymeric ionic liquid (PIL) microsphere, poly(1-vinyl-3-(2-methoxy-2-oxyl ethyl)imidazolium) hexafluorophosphate, is prepared via W/O emulsion polymerization. Rapid ion-exchange between the anionic moieties of PIL and DNA fragments is demonstrated facilitating the exchange equilibrium to be reached within 1 min. The PIL microspheres exhibit a high capacity of 190.7 μg mg(-1) for DNA adsorption. A fast DNA isolation protocol is thus developed with the PIL microspheres as solid phase adsorbent. It is feasible to facilitate DNA adsorption or stripping from the microspheres by simply regulating the concentration of salt. DNA adsorption is facilitated at low salt concentration, while higher concentration of salt entails DNA recovery from the microspheres. In practice, the retained DNA could be readily recovered with 1.0 mol L(-1) NaCl as stripping reagent, giving rise to a recovery of ca. 80.7%. The PIL microspheres are used for the adsorption/isolation of plasmid DNA from E. coli cell culture, demonstrating a superior adsorption performance with respect to that achieved by a commercial Plasmid Miniprep Kit.
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Box 332, Shenyang 110819, China; Department of Life Science and Technology, Yangtze Normal University, Chongqing 408100, China.
authors.forename: Ligang
authors.initials: L
authors.lastname: Xing
authors.affiliation: Institute of Biological Technology, Colleges of Sciences, Northeastern University, Shenyang 110189, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: chenxuwei@mail.neu.edu.cn.
authors.forename: Xuwei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Research Center for Analytical Sciences, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: jianhuajrz@mail.neu.edu.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D015394:Molecular Structure; D010957:Plasmids; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D013094:Spermatozoa
chemical_list: D007093:Imidazoles; D052578:Ionic Liquids; D011108:Polymers; D011145:Polyvinyls; D004247:DNA
keywords: Isolation; Plasmid DNA; Polymeric ionic liquid; Solid phase extraction