title: [Simultaneous determination of ten constituents in the Chinese medicinal preparation fufangxingxiangtu' erfeng capsules by ultra performance liquid chromatography with tandem mass spectrometry].
abstract: Using caffeic acid and icariin as internal standards, a method for the simultaneous determination of protocatechuic acid, protocatechuic aldehyde, chlorogenic acid, scutellarin, isochlorogenic acid C, baicalin, luteolin, apigenin, atractylenolide III and atractylenolide I in Fufangxingxiangtu' erfeng capsules were established by ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The separation was performed on a ZORBAX RRHD Eclipse Plus C18 column (50 mm x 2.1 mm, 1.8 microm) by using water containing 0.3% formic acid and methanol as mobile phases with the gradient elution at a flow rate of 0.3 mL/min. The analytes were detected by a tandem mass spectrometer in the multiple reaction monitoring (MRM) mode via the switching of positive electrospray ionization (ESI(+)) and negative electrospray ionization (ESI(-)). Under optimum conditions, the calibration curves were linear in the range of 0.003 00-24.0 mg/L for protocatechuic acid, 0.017 0-2.00 mg/L for protocatechuic aldehyde, 0.015 0-30.0 mg/L for chlorogenic acid, 0.004 00-30.0 mg/L for scutellarin, 0.010 5-24.0 mg/L for isochlorogenic acid C, 0.003 00-30.0 mg/L for baicalin, 0.003 00-5.0 mg/L for luteolin, 0.006 00-1.50 mg/L for apigenin, 0.001 50-4.00 mg/L for atractylenolide III, and 0.000 600-0.900 mg/L for atractylenolide I with the detection limits of 1.0, 11, 5.0, 1.5, 3.5, 1.0, 1.0, 2.0, 0.50, 0.20 microg/L, respectively. The average recoveries of the ten effective components were between 92.5% and 106% with all relative standard deviations not more than 3.2%. The developed method was rapid, simple, accurate, reproducible, and suitable for the quality control of the Fufangxingxiangtu' erfeng capsules.
authors.forename: Xiaosu
authors.initials: X
authors.lastname: Fan
authors.forename: Qian
authors.initials: Q
authors.lastname: Pang
authors.forename: Yuanjin
authors.initials: Y
authors.lastname: Xu
mesh_terms: D002109:Caffeic Acids; D002214:Capsules; D002726:Chlorogenic Acid / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005419:Flavonoids; D062385:Hydroxybenzoates; D053719:Tandem Mass Spectrometry
chemical_list: D002109:Caffeic Acids; D002214:Capsules; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D062385:Hydroxybenzoates; C469310:isochlorogenic acid; D002726:Chlorogenic Acid; C038044:baicalin; C009091:protocatechuic acid; C040048:caffeic acid; C056599:icariin