title: [Genetic characterization of the isolates of measles viruses in Hongkou district of Shanghai, China in 2012].
abstract: OBJECTIVE
  To ascertain the genotype of measles viruses isolated in 2012 and genetic characterization of measles viruses in Hongkou district of Shanghai during 2000-2012.
  
  
  METHODS
  Measles virus was isolated from throat swab specimens collected from suspected measles cases and 450 bp fragment of C terminus on nucleoprotein (N) gene was amplified by RT-PCR. Sequence analysis was conducted to ascertain the genotype and to compare the difference of nucleotide with other measles virus strain announced by GenBank during 2000-2012. Measles virus genotype was analyzed. Epidemiological investigation was conducted.
  
  
  RESULTS
  Phylogenetic analysis showed that 7 measles virus samples were isolated from 34 throat swab specimens with 6 of them belonged to H1 genotype, 1 belonged to D8 genotype of H1 genotype. H1a appeared the main part of Shanghai measles virus. Epidemiological survey showed that D8 was an imported case, also the first case detected since 2000.
  
  
  CONCLUSIONS
  The genotype distribution of measles virus in Hongkou was identified the same as elsewhere in Shanghai. D8 was an imported case, detected for the first time since 2000. The results suggested that viral gene sequencing and genotyping should be regularly conducted at the measles laboratories in Shanghai to strengthen the networking monitoring program of the disease.
authors.affiliation: Hongkou District Center for Disease Control and Prevention of Shanghai, Shanghai 200082, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Li
authors.affiliation: Hongkou District Center for Disease Control and Prevention of Shanghai, Shanghai 200082, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Tang
authors.affiliation: Hongkou District Center for Disease Control and Prevention of Shanghai, Shanghai 200082, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Qian
authors.affiliation: Shanghai Municipal Center for Disease Control and Prevention.
authors.forename: Zheng'an
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Shanghai Municipal Center for Disease Control and Prevention.
authors.forename: Chongshan
authors.initials: C
authors.lastname: Li
authors.affiliation: Hongkou District Center for Disease Control and Prevention of Shanghai, Shanghai 200082, China.
authors.forename: Yanji
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Hongkou District Center for Disease Control and Prevention of Shanghai, Shanghai 200082, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Yu
authors.affiliation: Hongkou District Center for Disease Control and Prevention of Shanghai, Shanghai 200082, China.
authors.forename: Xiangzhen
authors.initials: X
authors.lastname: Gong
mesh_terms: D000328:Adult; D002681:China / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008459:Measles virus / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014777:Virus Diseases / Q000235:genetics