title: Isolation, structural analyses and biological activity assays against chronic lymphocytic leukemia of two novel cytochalasins - sclerotionigrin A and B.
abstract: Two new cytochalasins, sclerotionigrin A (1) and B (2) were isolated together with the known proxiphomin (3) from the filamentous fungus Aspergillus sclerotioniger. The structures and relative stereochemistry of 1 and 2 were determined based on comparison with 3, and from extensive 1D and 2D NMR spectroscopic analysis, supported by high resolution mass spectrometry (HRMS). Compounds 2 and 3 displayed cytotoxic activity towards chronic lymphocytic leukemia cells in vitro, with 3 being the most active.
authors.affiliation: Department of Systems Biology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark. lmape@bio.dtu.dk.
authors.forename: Lene M
authors.initials: LM
authors.lastname: Petersen
authors.affiliation: Department of Systems Biology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark. ttb@bio.dtu.dk.
authors.forename: Tanja T
authors.initials: TT
authors.lastname: Bladt
authors.affiliation: German Cancer Research Center, Molecular Genetics, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany. C.Duerr@dkfz-heidelberg.de.
authors.forename: Claudia
authors.initials: C
authors.lastname: Dürr
authors.affiliation: German Cancer Research Center, Molecular Genetics, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany. M.Seiffert@dkfz-heidelberg.de.
authors.forename: Martina
authors.initials: M
authors.lastname: Seiffert
authors.affiliation: Department of Systems Biology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark. jcf@bio.dtu.dk.
authors.forename: Jens C
authors.initials: JC
authors.lastname: Frisvad
authors.affiliation: Department of Chemistry, Technical University of Denmark, Kemitorvet, B201, DK-2800 Kgs. Lyngby, Denmark. chg@kemi.dtu.dk.
authors.forename: Charlotte H
authors.initials: CH
authors.lastname: Gotfredsen
authors.affiliation: Department of Systems Biology, Technical University of Denmark, Søltofts Plads B221, DK-2800 Kgs. Lyngby, Denmark. tol@bio.dtu.dk.
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Larsen
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003572:Cytochalasins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D000970:Antineoplastic Agents; D003572:Cytochalasins; C000596160:sclerotionigrin B