title: Enhancing the lipolysis-stimulating activity of soy protein using limited hydrolysis with Flavourzyme and ultrafiltration.
abstract: The aim of this study was to explore the lipolysis-stimulating activity of soy protein isolate (SPI) hydrolysate using 3T3-L1 adipocytes. Intracellular triglyceride residue (TR) was employed as a marker for lipolysis in cells. The lower TR represents the better lipolysis-stimulating activity. SPI was hydrolysed with Flavourzyme for 2 h to obtain the hydrolysate FH2h, which showed lipolysis-stimulating activity in adipocytes at 400-1600 ppm levels. The sequential fractionation of FH2h with 30-0.3 kDa molecular weight cut-off (MWCO) membranes in order to obtain a 1 kDa retentate resulted in further enhancement of its lipolysis-stimulating activity in the cells. The TR decreased significantly from 2.73 to 2.30 μmole/mg protein at the 400 ppm level (p<0.05). Based on the western immunoblot and immunostaining analysis, the 1 kDa retentate promotes lipolysis by increasing phosphorylation and translocation of the hormone-sensitive lipase in 3T3-L1 adipocytes.
authors.affiliation: Department of Culinary Arts, Chung Chou University of Science and Technology, 6 Lane 3 Sec. 3 Shanchiao Road, Yuanlin, Changhua 51003, Taiwan, ROC.
authors.forename: May-June
authors.initials: MJ
authors.lastname: Tsou
authors.affiliation: Department of Food Science, National I-Lan University, 1 Shenlung Road, I-Lan 26015, Taiwan, ROC.
authors.forename: Shin-Bin
authors.initials: SB
authors.lastname: Lin
authors.affiliation: Department of Food Science, Tunghai University, 181 Sec. 3 Taichung Kan Road, Taichung 40704, Taiwan, ROC.
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Chao
authors.affiliation: Department of Food Science, Tunghai University, 181 Sec. 3 Taichung Kan Road, Taichung 40704, Taiwan, ROC.
authors.forename: Wen-Dee
authors.initials: WD
authors.lastname: Chiang
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D010450:Endopeptidases / Q000737:chemistry*; D006868:Hydrolysis; D008066:Lipolysis / Q000276:immunology*; D051379:Mice; D030262:Soybean Proteins / Q000737:chemistry*; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D030262:Soybean Proteins; D010450:Endopeptidases; C412470:flavourzyme