title: NADH dehydrogenase-like behavior of nitrogen-doped graphene and its application in NAD(+)-dependent dehydrogenase biosensing.
abstract: A novel electrochemical biosensing platform for nicotinamide adenine dinucleotide (NAD(+))-dependent dehydrogenase catalysis was designed using the nitrogen-doped graphene (NG), which had properties similar to NADH dehydrogenase (CoI). NG mimicked flavin mononucleotide (FMN) in CoI and efficiently catalyzed NADH oxidation. NG also acted as an electron transport "bridge" from NADH to the electrode due to its excellent conductivity. In comparison with a bare gold electrode, an 800 mV decrease in the overpotential for NADH oxidation and CoI-like behavior were observed at NG-modified electrode, which is the largest decrease in overpotential for NADH oxidation reported to date. The catalytic rate constant (k) for the CoI-like behavior of NG was estimated to be 2.3×10(5) M(-1) s(-1), which is much higher than that of other previously reported FMN analogs. The Michaelis-Menten constant (Km) of NG was 26 μM, which is comparable to the Km of CoI (10 μM). Electrodes modified with NG and NG/gold nanoparticals/formate dehydrogenase (NG/AuNPs/FDH) showed excellent analytical performance for the detection of NADH and formate. This electrode fabrication strategy could be used to create a universal biosensing platform for developing NAD(+)-dependent dehydrogenase biosensors and biofuel cells.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Gai
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Cui-E
authors.initials: CE
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Petrochemical Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: E S
authors.initials: ES
authors.lastname: Abdel-Halim
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China; School of Chemistry and Life Science, Nanjing University Jinling College, Nanjing 210089, PR China. Electronic address: jrzhang@nju.edu.cn.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, PR China. Electronic address: jjzhu@nju.edu.cn.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques; D005486:Flavin Mononucleotide; D005560:Formate Dehydrogenases; D005561:Formates / Q000032:analysis; D006108:Graphite; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD; D009245:NADH Dehydrogenase; D009584:Nitrogen; D010084:Oxidation-Reduction; D010088:Oxidoreductases
chemical_list: D005561:Formates; D009243:NAD; C030544:formic acid; D006108:Graphite; D005486:Flavin Mononucleotide; D010088:Oxidoreductases; D005560:Formate Dehydrogenases; D009245:NADH Dehydrogenase; D009584:Nitrogen
keywords: Biosensing platform; NAD(+)-dependent dehydrogenase; NADH dehydrogenase (CoI)-like behavior of nitrogen-doped graphene