title: Promicromonospora iranensis sp. nov., an actinobacterium isolated from rhizospheric soil.
abstract: A novel strain of the genus Promicromonospora, designated HM 792(T), was isolated from soil in Fars Province, Iran. On ISP 2 medium, the yellow-pigmented isolate produced long and branched hyphae that developed into a large number of irregularly shaped spores. It showed growth at 25-30 °C and pH 6.0-9.0 with 0-8 % (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Promicromonospora. Whole-cell hydrolysates of strain HM 792(T) contained the amino acids d-glutamic acid, l-alanine and l-lysine along with the sugars glucose and ribose. The main polar lipids were diphosphatidylglycerol, two unknown phospholipids, two unknown glycolipids and two unknown phosphoglycolipids, complemented by minor concentrations of phosphatidylinositol and phosphatidylglycerol. MK-9(H4) was the predominant menaquinone. The fatty-acid pattern was composed mainly of the saturated branched-chain acids anteiso-C15 : 0 and iso-C15 : 0. 16S rRNA gene sequence analysis showed the highest pairwise sequence identity (96.6-99.0 %) with the members of the genus Promicromonospora. Based on phenotypic and genotypic features, strain HM 792(T) is considered to represent a novel species of the genus Promicromonospora, for which the name Promicromonospora iranensis sp. nov. is proposed. Strain HM 792(T) ( = DSM 45554(T) = UTMC00792(T) = CCUG 63022(T)) is the type strain.
authors.affiliation: University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research, Center, University of Tehran, Tehran, Iran.|Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455 Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mohammadipanah
authors.affiliation: University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research, Center, University of Tehran, Tehran, Iran.|Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455 Tehran, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Hamedi
authors.affiliation: Leibniz-Institut DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, 38124 Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Leibniz-Institut DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, 38124 Braunschweig, Germany.
authors.forename: María Del Carmen
authors.initials: MDC
authors.lastname: Montero-Calasanz
authors.affiliation: Leibniz-Institut DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, 38124 Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Leibniz-Institut DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, 38124 Braunschweig, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007492:Iran; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9