title: Zebrafish as a new model for phenotype-based screening of positive inotropic agents.
abstract: The zebrafish-based assay is a widely used animal model system for cardiovascular research. In this study, we investigated the cardiac defects caused by terfenadine and tested the pharmacological response of digoxin to zebrafish with cardiac defects. The study suggested that zebrafish could be a suitable model for phenotype-based screening and evaluation of positive inotropic agents. This phenotype-based heart failure zebrafish model system was then utilized in in-house library screen. Some positive inotropic compound was discovered, which could attenuate the cardiac defects by increasing the flow velocity of caudal artery blood.
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Tang
authors.forename: Desheng
authors.initials: D
authors.lastname: Xie
authors.forename: Bainian
authors.initials: B
authors.lastname: Feng
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002316:Cardiotonic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D004077:Digoxin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000502:physiology; D006321:Heart / Q000187:drug effects / Q000502:physiology; D006333:Heart Failure / Q000139:chemically induced / Q000188:drug therapy*; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000473:pathology; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D016593:Terfenadine / Q000633:toxicity; D015027:Zebrafish / Q000254:growth & development
chemical_list: D002316:Cardiotonic Agents; D004077:Digoxin; D016593:Terfenadine
keywords: heart failure; phenotype-based; positive inotropic agent; zebrafish