title: Very fast capillary electrophoresis with electrochemical detection for high-throughput analysis using short, vertically aligned capillaries.
abstract: A method for conducting fast and efficient capillary electrophoresis (CE) based on short separation capillaries in vertical alignment was developed. The strategy enables for high-throughput analysis from small sample vials (low microliter to nanoliter range). The system consists of a lab-made miniaturized autosampling unit and an amperometric end-column detection (AD) cell. The device enables a throughput of up to 200 separations per hour. CE-AD separations of a dye model system in capillaries of only 4 to 7.5 cm length with inner diameters (ID) of 10 or 15 μm were carried out under conditions of very high electric field strengths (up to 3.0 kV/cm) with high separation efficiency (half peak widths below 0.2 s) in less than 3.5 s migration time. A non-aqueous background electrolyte, consisting of 10 mM ammonium acetate and 1 M acetic acid in acetonitrile, was used. The practical suitability of the system was evaluated by applying it to the determination of dyes in overhead projector pens.
authors.affiliation: University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, Universitätsstrasse 31, 93040, Regensburg, Germany.
authors.forename: Jonas Josef Peter
authors.initials: JJ
authors.lastname: Mark
authors.forename: Paolo
authors.initials: P
authors.lastname: Piccinelli
authors.forename: Frank-Michael
authors.initials: FM
authors.lastname: Matysik
mesh_terms: D004396:Coloring Agents / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*
chemical_list: D004396:Coloring Agents