title: Plasmonic ELISA for the detection of gp120 at ultralow concentrations with the naked eye.
abstract: The technique of plasmonic ELISA is utilised here to detect the HIV-1 protein gp120 with the ultralow limit of detection of 8 × 10(-20) M (10(-17) g mL(-1)) in an independent laboratory. It was corroborated that changes in the concentration of hydrogen peroxide as small as 0.05 μM could lead to nanoparticle solutions of completely different tonality.
authors.affiliation: Department of Chemistry, The MRC/UCL Centre for Medical Molecular Virology, University College London, 20 Gordon Street, London, WC1H 0AJ, UK. g.battaglia@ucl.ac.uk.
authors.forename: D
authors.initials: D
authors.lastname: Cecchin
authors.forename: R
authors.initials: R
authors.lastname: de la Rica
authors.forename: R E S
authors.initials: RE
authors.lastname: Bain
authors.forename: M W
authors.initials: MW
authors.lastname: Finnis
authors.forename: M M
authors.initials: MM
authors.lastname: Stevens
authors.forename: G
authors.initials: G
authors.lastname: Battaglia
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015699:HIV Envelope Protein gp120 / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000378:metabolism; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D016012:Poisson Distribution
chemical_list: D015699:HIV Envelope Protein gp120; D006861:Hydrogen Peroxide