title: Preparation and characterization of brush-like PEGMA-graft-PDA coating and its application for protein separation by CE.
abstract: In this paper, a novel brush-like copolymer consisting of poly(ethylene glycol) methyl ether methacrylate and 2-aminoethyl methacrylate (AEMA) named as poly(PEGMA300-co-AEMA) was synthesized by atom transfer radical polymerization (ATRP), and then, poly(PEGMA300-co-AEMA) copolymer was immobilized onto material surfaces through polydopamine (PDA)-anchored coating. The defined copolymer structure was characterized by nuclear magnetic resonance hydrogen spectroscopy ((1)H NMR) and gel permeation chromatography (GPC). The chemical component and surface morphology of the brush-like copolymer-graft-PDA coating were studied by using X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM), respectively. The hydrophilicity of the brush-like copolymer-graft-PDA coating was investigated by using static water contact angle. The protein-resistant property of the brush-like copolymer-graft-PDA coating was investigated by using quartz crystal microbalance with dissipation (QCM-D), and finally the coating was applied to capillary inner surface for protein separation by capillary electrophoresis (CE).
authors.affiliation: a CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering , University of Science and Technology of China , Hefei 230026 , P.R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
authors.forename: Guangming
authors.initials: G
authors.lastname: Liu
authors.forename: Songtao
authors.initials: S
authors.lastname: Liu
authors.forename: Longchao
authors.initials: L
authors.lastname: Bai
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002417:Cattle; D004355:Drug Stability; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000379:methods*; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D007211:Indoles; D008689:Methacrylates; D011108:Polymers; D011506:Proteins; C568283:polydopamine; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; C072703:2-aminoethylmethacrylate
keywords: brush-like copolymer; polydopamine; protein resistance; protein separation