title: Enzyme-linked immunosorbent assay for the determination of five organophosphorus pesticides in camellia oil.
abstract: A matrix solid-phase dispersion and direct competitive enzyme-linked immunosorbent assay (MSPD-ELISA) was developed for five organophosphorus pesticides (OPs) in camellia oil. Seven haptens with different substituents in the aromatic ring were used to prepare different competitors; the ELISA showed highest sensitivity and specificity to OPs when the competitor had moderate heterology to the immunizing hapten. Several assay conditions were optimized to increase the ELISA sensitivity. The optimized ELISA for five OPs had 50% inhibitory concentrations of 6.3 ng/ml (parathion), 18.9 ng/ml (methyl parathion), 120.7 ng/ml (fenitrothion), 110.4 ng/ml (fenthion), and 20.7 ng/ml (phoxim). The average recoveries of five OPs in camellia oil ranged from 75.7 to 105.3%, with the interassay coefficient of variations ranging from 6.0 to 13.4%. Compared with the results previously reported, the ELISA that was developed in the present study showed a much higher sensitivity. Additionally, MSPD was used in the sample preparation to minimize the matrix effect. Recoveries from the method developed here were in agreement with those obtained by gas chromatography, which indicated that the detection performance of the MSPD-ELISA could meet the regulatory requirements of different governments and international organizations.
authors.affiliation: Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, People's Republic of China; Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, People's Republic of China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, People's Republic of China.
authors.forename: Yirong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, People's Republic of China.
authors.forename: Guonian
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, People's Republic of China. yalin_zj@163.com.
authors.forename: Fubin
authors.initials: F
authors.lastname: Tang
mesh_terms: D028244:Camellia / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D010938:Plant Oils / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D009943:Organophosphorus Compounds; D010575:Pesticides; D010938:Plant Oils