title: [Alanine solution as enzyme reaction buffer used in A to O blood group conversion].
abstract: The aim of this study was to investigate the effect of alanine solution as α-N-acetylgalactosaminidase enzyme reaction buffer on the enzymatic activity of A antigen. The binding ability of α-N-acetylgalactosaminidase with RBC in different reaction buffer such as alanine solution, glycine solution, normal saline (0.9% NaCl), PBS, PCS was detected by Western blot. The results showed that the efficiency of A to O conversion in alanine solution was similar to that in glycine solution, and Western blot confirmed that most of enzymes blinded with RBC in glycine or alanine solution, but few enzymes blinded with RBC in PBS, PCS or normal saline. The evidences indicated that binding of enzyme with RBC was a key element for A to O blood group conversion, while the binding ability of α-N-acetylgalactosaminidase with RBC in alanine or glycine solution was similar. It is concluded that alanine solution can be used as enzyme reaction buffer in A to O blood group conversion. In this buffer, the α-N-acetylgalactosaminidase is closely blinded with RBC and α-N-acetylgalactosaminidase plays efficient enzymatic activity of A antigen.
authors.affiliation: Department of Blood Molecular Biology,Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Su-Bo
authors.initials: SB
authors.lastname: Li
authors.affiliation: Department of Blood Molecular Biology,Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 2Shenzhen Transfusion Medicine Institute, Shenzhen 518035, Guangdong Province, China. E-mail: zyz200157@sohu.com.
authors.forename: Yin-Ze
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Ying-Xia
authors.initials: YX
authors.lastname: Tan
authors.affiliation: Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Bao
authors.affiliation: Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Ying-Li
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Department of Blood Molecular Biology,Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Shou-Ping
authors.initials: SP
authors.lastname: Ji
authors.affiliation: Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gong
authors.affiliation: Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China. E-mail:gaohongwei1976@126.com.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Gao
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000409:Alanine; D001788:Blood Grouping and Crossmatching / Q000379:methods; D006801:Humans; D012996:Solutions; D048809:alpha-N-Acetylgalactosaminidase / Q000276:immunology*
chemical_list: D000017:ABO Blood-Group System; D012996:Solutions; D048809:alpha-N-Acetylgalactosaminidase; D000409:Alanine