title: Mucoactive effects of naringin in lipopolysaccharide-induced acute lung injury mice and beagle dogs.
abstract: Our previous study has demonstrated that naringin attenuates EGF-induced MUC5AC hypersecretion in A549 cells by suppressing the cooperative activities of MAPKs/AP-1 and IKKs/IκB/NF-κB signaling pathways. However, the volume of airway mucus is determined by two factors including the number of mucous cells and capacity of mucus secretion. The aim of the present study is to explore the mucoactive effects of naringin in lipopolysaccharide (LPS)-induced acute lung injury (ALI) mice and beagle dogs. The results demonstrated that naringin of 12.4 mg/kg treatment significantly decreased LPS-induced enhancement of sputum volume and pulmonary inflammation, remarkably increased the subglottic sputum volume and solids content in sputum of lower trachea, while partially, but not fully, significantly increased the elasticity and viscosity of sputum in lower trachea of beagle dogs. Moreover, the MUC5AC content in BALF and goblet-cells in large airways of LPS-induced ALI mice were significantly attenuated by dexamethasone (5 mg/kg), ambroxol (25 mg/kg), and naringin (15, 60 mg/kg). However, the goblet-cells hyperplasia in small airways induced by LPS was only significantly inhibited by dexamethasone and naringin (60 mg/kg). In conclusion, naringin exhibits mucoactive effects through multiple targets which including reduction of goblet cells hyperplasia and mucus hypersecretion, as well as promotion of sputum excretion.
authors.affiliation: Guangzhou Quality R&D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Chinese Medicine, The University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Guangzhou Quality R&D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Yi-chu
authors.initials: YC
authors.lastname: Nie
authors.affiliation: Guangzhou Quality R&D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Pei-bo
authors.initials: PB
authors.lastname: Li
authors.affiliation: School of Chinese Medicine, The University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: shenjg@hku.hk.
authors.forename: Jian-gang
authors.initials: JG
authors.lastname: Shen
authors.affiliation: Guangzhou Quality R&D Center of Traditional Chinese Medicine, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China. Electronic address: lsssww@126.com.
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Su
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D004285:Dogs; D004548:Elasticity; D005260:Female; D044950:Flavanones / Q000494:pharmacology*; D020397:Goblet Cells / Q000187:drug effects / Q000473:pathology; D006965:Hyperplasia / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism; D016209:Interleukin-8 / Q000276:immunology; D007958:Leukocyte Count; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D055271:Mucin 5AC / Q000378:metabolism; D009093:Mucus / Q000378:metabolism*; D011014:Pneumonia / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D013183:Sputum / Q000737:chemistry / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D014783:Viscosity
chemical_list: D044950:Flavanones; D016209:Interleukin-8; D008070:Lipopolysaccharides; C524679:Muc5ac protein, mouse; D055271:Mucin 5AC; D014409:Tumor Necrosis Factor-alpha; C005274:naringin
keywords: Acute lung injury; Beagle dogs; LPS; Mice; Mucoactive effect; Naringin