title: Simultaneous quantification of three pyranocoumarins of Peucedanum praeruptorum in rat plasma by liquid chromatography-tandem mass spectrometry: application to pharmacokinetic study.
abstract: A simple, rapid and robust liquid chromatography-tandem mass spectrometry was established and validated for simultaneous quantifications of three pyranocoumarins (praeruptorin A-C) in rat plasma. Following a single-step liquid-liquid extraction, the analytes were separated on a reversed-phase C18 column with a mobile phase consisting of methanol and 10 mM ammonium acetate solution (70 : 30, v/v) at a constant flow rate of 0.3 mL/min. The linear calibration curves were obtained over the concentration ranges 2.93-1470 ng/mL for praeruptorin A, 1.47-734 ng/mL for praeruptorin B and 2.00-1000 ng/mL for praeruptorin C. The within-batch accuracy was -8.6 to 7.5% for praeruptorin A, -9.5 to 12.0% for praeruptorin B and -10.5 to 12.5% for praeruptorin C, respectively. The between-batch accuracy was -3.5 to 1.4% for praeruptorin A, -8.7 to 3.4% for praeruptorin B and -6.0 to 4.3% for praeruptorin C, respectively. The within-batch and between-batch precisions were ≤13.1 and ≤8.2%, respectively. This method is suitable to simultaneously determine the three pyranocoumarins in plasma and thus to investigate the pharmacokinetics of the pyranocoumarins of Peucedanum praeruptorum in rats.
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai 264000, PR China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai 264000, PR China.
authors.forename: Guozhen
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai 264000, PR China hbliu28@outlook.com.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D039681:Pyranocoumarins / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010936:Plant Extracts; D039681:Pyranocoumarins