title: Vitamin E-diffused highly cross-linked UHMWPE particles induce less osteolysis compared to highly cross-linked virgin UHMWPE particles in vivo.
abstract: Recent in vitro findings suggest that UHMWPE wear particles containing vitamin E (VE) may have reduced biologic activity and decreased osteolytic potential. We hypothesized that particles from VE-stabilized, radiation cross-linked UHMWPE would cause less osteolysis in a murine calvarial bone model when compared to virgin gamma irradiated cross-linked UHMWPE. Groups received equal amount of particulate debris overlaying the calvarium for 10 days. Calvarial bone was examined using high resolution micro-CT and histomorphometric analyses. There was a statistically significant difference between virgin (12.2%±8%) and VE-UHMWPE (3%±1.4%) groups in regards to bone resorption (P=0.005) and inflammatory fibrous tissue overlaying the calvaria (0.48 vs. 0.20, P<0.0001). These results suggest that VE-UHMWPE particles have reduced osteolytic potential in vivo when compared to virgin UHMWPE.
authors.affiliation: Harris Orthopaedic Laboratory, Massachusetts General Hospital, Boston, Massachusetts; Harvard Medical School, Boston, Massachusetts.
authors.forename: David A
authors.initials: DA
authors.lastname: Bichara
authors.affiliation: Harris Orthopaedic Laboratory, Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: Erik
authors.initials: E
authors.lastname: Malchau
authors.affiliation: Harris Orthopaedic Laboratory, Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: Nanna H
authors.initials: NH
authors.lastname: Sillesen
authors.affiliation: Harris Orthopaedic Laboratory, Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: Selami
authors.initials: S
authors.lastname: Cakmak
authors.affiliation: Harvard Medical School, Boston, Massachusetts; Bone and Soft Tissue Pathology, Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: G Petur
authors.initials: GP
authors.lastname: Nielsen
authors.affiliation: Harris Orthopaedic Laboratory, Massachusetts General Hospital, Boston, Massachusetts; Harvard Medical School, Boston, Massachusetts.
authors.forename: Orhun K
authors.initials: OK
authors.lastname: Muratoglu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005720:Gamma Rays; D008297:Male; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D010014:Osteolysis / Q000209:etiology* / Q000517:prevention & control*; D010316:Particle Size; D011095:Polyethylenes / Q000494:pharmacology*; D011897:Random Allocation; D012886:Skull / Q000000981:diagnostic imaging / Q000473:pathology*; D014810:Vitamin E / Q000494:pharmacology*; D055114:X-Ray Microtomography
chemical_list: D011095:Polyethylenes; C111601:ultra-high molecular weight polyethylene; D014810:Vitamin E
keywords: UHMWPE; murine calvarial model; particle-induced osteolysis; polyethylene wear debris; vitamin E