title: Food entrains clock genes but not metabolic genes in the liver of suprachiasmatic nucleus lesioned rats.
abstract: Hepatic circadian transcription, considered to be driven by the liver clock, is largely influenced by food even uncoupling it from the suprachiasmatic nucleus (SCN). In SCN lesioned rats (SCNx) we determined the influence of a physiological feeding schedule on the entrainment of clock and clock-controlled (CCG) genes in the liver. We show that clock genes and the CCG Rev-erbα and peroxisome proliferator-activated receptor alpha (PPARα) in food-scheduled intact and SCNx have a robust diurnal differential expression persisting after a 24h fast. However, hepatic nicotinamide phosphoribosyl transferase (Nampt) shows time dependent changes that are lost in intact animals under fasting; moreover, it is unresponsive to the nutrient status in SCNx, indicating a poor reliance on liver clock genes and highlighting the relevance of SCN-derived signals for its metabolic status-related expression.
authors.affiliation: Department of Cell Biology and Physiology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Sabath
authors.affiliation: Department of Cell Biology and Physiology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Salgado-Delgado
authors.affiliation: Department of Cell Biology and Physiology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Natali N
authors.initials: NN
authors.lastname: Guerrero-Vargas
authors.affiliation: Department of Cell Biology and Physiology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Mara A
authors.initials: MA
authors.lastname: Guzman-Ruiz
authors.affiliation: Department of Cell Biology and Physiology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Maria
authors.initials: M
authors.lastname: del Carmen Basualdo
authors.affiliation: Department of Anatomy, Faculty of Medicine, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Carolina
authors.initials: C
authors.lastname: Escobar
authors.affiliation: Department of Cell Biology and Physiology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, Mexico City, Mexico. Electronic address: ruudbuijs@gmail.com.
authors.forename: Ruud M
authors.initials: RM
authors.lastname: Buijs
mesh_terms: D000818:Animals; D057906:Circadian Clocks / Q000235:genetics*; D005215:Fasting / Q000378:metabolism; D005502:Food; D005786:Gene Expression Regulation; D051797:Inhibitor of Differentiation Protein 2 / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D054409:Nicotinamide Phosphoribosyltransferase / Q000235:genetics; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1 / Q000235:genetics; D047493:PPAR alpha / Q000235:genetics; D051381:Rats; D013493:Suprachiasmatic Nucleus / Q000503:physiopathology*; D013696:Temperature
chemical_list: C497451:Id2 protein, rat; D051797:Inhibitor of Differentiation Protein 2; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1; D047493:PPAR alpha; D054409:Nicotinamide Phosphoribosyltransferase
keywords: Clock genes; Food-restriction; Inhibitor of DNA binding 2; Nicotinamide phosphoribosyl transferase; Peroxisome proliferator-activated receptor alpha; Sirt1