title: Super-resolution fluorescence imaging of biocompatible carbon dots.
abstract: Carbon Dots (CDs) are a new promising type of small (5 nm), biocompatible and multicolor luminescent nanoparticle. Here, we demonstrate super-resolution imaging of CDs at the nanoscale through STimulated Emission Depletion (STED) microscopy. In addition, we report the application of STED for detection of CD localization in both fixed and living cells, achieving a spatial resolution down to 30 nm, far below the diffraction limit, showing great promise for high resolution visualization of cellular dynamics.
authors.affiliation: Istituto Italiano di Tecnologia, Center for Bio-Molecular Nanotechnologies@UniLe, Via Barsanti - 73010, Arnesano (Lecce), Italy. pierpaolo.pompa@iit.it.
authors.forename: Godefroy
authors.initials: G
authors.lastname: Leménager
authors.forename: Elisa
authors.initials: E
authors.lastname: De Luca
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Sun
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Pompa
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D006801:Humans; D049449:Luminescence; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D055095:Optics and Photonics; D010777:Photochemistry
chemical_list: D001672:Biocompatible Materials; D002244:Carbon