title: Measuring zeta potential of protein nano-particles using electroacoustics.
abstract: Electroacoustic spectroscopy offers a simple way for measuring the zeta potential of proteins in physiological solutions with high ionic strength. Ultrasound as a driving force does not generate the heat effects which complicate traditional electrophoretic measurements at high ionic strength. In addition, measurements can be conducted with concentrated protein dispersions without dilution, as is required by electrophoretic methods. This paper presents results for electroacoustic measurements of 5wt.% bovine serum albumin suspended in aqueous solutions. In these suspensions the proteins are not completely dissolved; they form nano-particles with a median size of about 180nm. We studied the dependence of zeta potential on ionic strength within a wide range of salt molarities, up to as high as 0.5mol/L. Dialysis was used for performing measurements at lower ionic strength range. We also conducted pH titrations of this system and titrations with Ca(2+) ions. Our results agree well with published data for samples where such data is available.
authors.affiliation: Dispersion Technology Inc, 364 Adams Street, Bedford Hills, NY 10507, USA. Electronic address: adukhin@dispersion.com.
authors.forename: A S
authors.initials: AS
authors.lastname: Dukhin
authors.affiliation: Dispersion Technology Inc, 364 Adams Street, Bedford Hills, NY 10507, USA.
authors.forename: S
authors.initials: S
authors.lastname: Parlia
mesh_terms: D000162:Acoustics; D000818:Animals; D002417:Cattle; D004553:Electric Conductivity; D055664:Electrochemical Techniques / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry*; D009994:Osmolar Concentration; D010316:Particle Size; D011189:Potassium Chloride / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D055672:Static Electricity; D017186:Titrimetry
chemical_list: D012710:Serum Albumin, Bovine; D011189:Potassium Chloride
keywords: Calcium ion; High ionic strength; Protein; Protein zeta potential; Zeta potential; pH titration