title: Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34.
abstract: The phage-proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of the 1289 amino-acid gene product 34 (gp34). Different carboxy-terminal parts of gp34 have been produced and crystallized. Crystals of gp34(726-1289) diffracting X-rays to 2.9 Å resolution, crystals of gp34(781-1289) diffracting to 1.9 Å resolution and crystals of gp34(894-1289) diffracting to 3.0 and 2.0 Å resolution and belonging to different crystal forms were obtained. Native data were collected for gp34(726-1289) and gp34(894-1289), while single-wavelength anomalous diffraction data were collected for selenomethionine-containing gp34(781-1289) and gp34(894-1289). For the latter, high-quality anomalous signal was obtained.
authors.affiliation: Departamento de Estructura de Macromoleculas, Centro Nacional de Biotecnologia (CNB-CSIC), Calle Darwin 3, 28046 Madrid, Spain.
authors.forename: Meritxell
authors.initials: M
authors.lastname: Granell
authors.affiliation: Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, B-9 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Mikiyoshi
authors.initials: M
authors.lastname: Namura
authors.affiliation: Departamento de Estructura de Macromoleculas, Centro Nacional de Biotecnologia (CNB-CSIC), Calle Darwin 3, 28046 Madrid, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Alvira
authors.affiliation: Departamento de Estructura de Macromoleculas, Centro Nacional de Biotecnologia (CNB-CSIC), Calle Darwin 3, 28046 Madrid, Spain.
authors.forename: Carmela
authors.initials: C
authors.lastname: Garcia-Doval
authors.affiliation: Departamento de Estructura de Macromoleculas, Centro Nacional de Biotecnologia (CNB-CSIC), Calle Darwin 3, 28046 Madrid, Spain.
authors.forename: Abhimanyu K
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Unit for Virus Host-Cell Interactions, Université Grenoble Alpes-EMBL-CNRS, 6 Rue Jules Horowitz, 38042 Grenoble, France.
authors.forename: Irina
authors.initials: I
authors.lastname: Gutsche
authors.affiliation: Departamento de Estructura de Macromoleculas, Centro Nacional de Biotecnologia (CNB-CSIC), Calle Darwin 3, 28046 Madrid, Spain.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: van Raaij
authors.affiliation: Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, B-9 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Kanamaru
mesh_terms: D017122:Bacteriophage T4 / Q000737:chemistry* / Q000378:metabolism; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012645:Selenomethionine / Q000737:chemistry*; D017376:Viral Tail Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D017376:Viral Tail Proteins; D012645:Selenomethionine
keywords: bacteriophage T4; gene product 34 (gp34)