title: LASP-1 promotes tumor proliferation and metastasis and is an independent unfavorable prognostic factor in gastric cancer.
abstract: OBJECTIVE
  The LIM and SH3 protein 1 (LASP-1) is a focal adhesion protein, and its expression has been reported to be increased in many malignant tumors. However, the role of LASP-1 in gastric cancer is still unknown. The aim of this study was to determine the relationship of LASP-1 expression with the progression and prognosis of gastric cancer.
  
  
  METHODS
  Expression of LASP-1 was evaluated in gastric cancer tissues and cell lines by immunohistochemistry and Western blot analysis. The relationship between LASP-1 expression and clinicopathological characteristics was analyzed. Using RNA interference, the effects of LASP-1 on cell proliferation, migration and invasion were investigated in gastric cancer cell lines both in vitro and in vivo.
  
  
  RESULTS
  The LASP-1 was overexpressed in gastric cancer tissues and cell lines. LASP-1 expression was significantly associated with tumor size, invasive depth, TNM stage, lymph node metastasis and p53 expression (all P < 0.05). Multivariate survival analysis showed that LASP-1 expression was recognized as an independent prognostic factor of patient's survival. Knockdown of LASP-1 inhibited cell proliferation, migration and invasion in vitro as well as tumorigenesis and metastasis in vivo.
  
  
  CONCLUSIONS
  Our study showed that LASP-1, overexpressed in gastric cancer and associated with poor prognosis, plays an important role in the growth and metastasis of gastric cancer.
authors.affiliation: Department of Pathology, Weifang Medical University, Baotong Street No. 7166, Weifang, 261053, Shandong, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Shuna
authors.initials: S
authors.lastname: Yu
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Qiao
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhang
authors.forename: Shujuan
authors.initials: S
authors.lastname: Liang
authors.forename: Hailiang
authors.initials: H
authors.lastname: Wang
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Liu
authors.forename: Fenghua
authors.initials: F
authors.lastname: Zhou
authors.forename: Jiying
authors.initials: J
authors.lastname: Jiang
authors.forename: Shijun
authors.initials: S
authors.lastname: Lu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000502:physiology*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D060588:LIM Domain Proteins / Q000502:physiology*; D008207:Lymphatic Metastasis; D008297:Male; D016513:Mice, SCID; D008875:Middle Aged; D009368:Neoplasm Transplantation; D011379:Prognosis; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C096353:LASP1 protein, human; D060588:LIM Domain Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53