title: Ethanol Extract of Alismatis rhizome Inhibits Adipocyte Differentiation of OP9 Cells.
abstract: The rhizome of Alisma orientale (Alismatis rhizome) has been used in Asia for promoting diuresis to eliminate dampness from the lower-jiao and to expel heat. In this study, an ethanol extract of the rhizome of Alisma orientale (AOE) was prepared and its effects on adipocyte differentiation of OP9 cells were investigated. Treatment with AOE in a differentiation medium for 5 days resulted in dose-dependent inhibition of lipid droplet formation in OP9 cells. Furthermore, AOE significantly inhibited adipocyte differentiation by downregulating the expression of the master transcription factor of adipogenesis, peroxisome proliferation-activity receptor γ (PPAR γ ), and related genes, including CCAAT/enhancer binding protein β (C/EBP β ), fatty acid-binding protein (aP2), and fatty acid synthase (FAS). AOE exerted its inhibitory effects primarily during the early adipogenesis stage (days 1-2), at which time it also exerted dose-dependent inhibition of the expression of C/EBP β , a protein related to the inhibition of mitotic clonal expansion. Additionally, AOE decreased the expression of autophagy-related proteins, including beclin 1, and the autophagy-related genes, (Atg) 7 and Atg12. Our results indicate that AOE's inhibitory effects on adipocyte differentiation of OP9 cells are mediated by reduced C/EBP β expression, causing inhibition of mitotic clonal expansion and autophagy.
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Park
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Mi-Seong
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea ; BK21 Plus program & Department of Smart Life-Care Convergence, Wonkwang University, Graduate School, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Ha-Rim
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Jeong-Mi
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Jin-Ki
authors.initials: JK
authors.lastname: Hwang
authors.affiliation: Department of Internal Medicine, Wonkwang University School of Medicine, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Sei-Hoon
authors.initials: SH
authors.lastname: Yang
authors.affiliation: Department of Family Medicine, The Catholic University of Korea, Incheon St. Mary's Hospital, 56 Dongsu-ro, Bupyeong-gu, Incheon 403-720, Republic of Korea.
authors.forename: Hye-Jung
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Hanbang Body-Fluid Research Center, Wonkwang University, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Dong-Sung
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Hanbang Body-Fluid Research Center, Wonkwang University, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea ; Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea ; Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Hyuncheol
authors.identifier: 0000-0001-9394-4358
authors.initials: H
authors.lastname: Oh
authors.affiliation: Hanbang Body-Fluid Research Center, Wonkwang University, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea ; Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea ; Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.affiliation: Department of Korean Physiology, Wonkwang University School of Korean Medicine, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Do-Gon
authors.initials: DG
authors.lastname: Ryu
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea ; BK21 Plus program & Department of Smart Life-Care Convergence, Wonkwang University, Graduate School, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea ; Department of Oral Biochemistry, and Institute of Biomaterials-Implant, Wonkwang University School of Dentistry, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Young-Rae
authors.initials: YR
authors.lastname: Lee
authors.affiliation: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea ; BK21 Plus program & Department of Smart Life-Care Convergence, Wonkwang University, Graduate School, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea ; Department of Korean Physiology, Wonkwang University School of Korean Medicine, 460 Iksan-daero, Iksan City, Jeonbuk 570-749, Republic of Korea.
authors.forename: Kang-Beom
authors.initials: KB
authors.lastname: Kwon