title: Acetone-butanol-ethanol (ABE) production by Clostridium beijerinckii from wheat straw hydrolysates: efficient use of penta and hexa carbohydrates.
abstract: ABE fermentation by Clostridium beijerinckii of steam-exploded and ozonated wheat straw hydrolysates was investigated. In steam-exploded hydrolysates, highest yields of 0.40 g/g ABE yield and 127.71 g ABE/kg wheat straw were achieved when the whole slurry from the pretreatment was used. In ozonated hydrolysates, 0.32 g/g ABE yield and 79.65 g ABE/kg wheat straw were obtained from washed ozonated wheat straw. Diverse effects were observed in steam explosion and ozonolysis of wheat straw which resulted in hemicellulose removal and acid insoluble lignin solubilization, respectively. SEM analysis showed structural differences in untreated and pretreated biomass. Depending on the operational strategy, after pretreatment and enzymatic hydrolysis, the glucose recovery ranged between 65.73-66.49% and 63.22-65.23% and the xylose recovery ranged between 45.19-61.00% and 34.54-40.91% in steam-exploded and ozonated hydrolysates, respectively. The effect of the main inhibitory compounds found in hydrolysates (oxalic acid, acetic acid, 5-hydroxymethylfurfural and furfural) was studied through ABE fermentation in model media.
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s/n, 47011 Valladolid, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Bellido
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s/n, 47011 Valladolid, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Loureiro Pinto
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s/n, 47011 Valladolid, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Coca
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s/n, 47011 Valladolid, Spain.
authors.forename: Gerardo
authors.initials: G
authors.lastname: González-Benito
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s/n, 47011 Valladolid, Spain. Electronic address: maite@iq.uva.es.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: García-Cubero
mesh_terms: D000096:Acetone / Q000378:metabolism*; D000440:Butanols / Q000378:metabolism*; D002241:Carbohydrates / Q000737:chemistry*; D046970:Clostridium beijerinckii / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D010126:Ozone / Q000494:pharmacology; D012995:Solubility; D013227:Steam; D014908:Triticum / Q000187:drug effects / Q000378:metabolism*; D014866:Waste Products
chemical_list: D000440:Butanols; D002241:Carbohydrates; D013227:Steam; D014866:Waste Products; D000096:Acetone; D000431:Ethanol; D010126:Ozone
keywords: ABE fermentation; Clostridium beijerinckii; Ozone pretreatment; Steam explosion; Wheat straw