title: Total flavonoids from Ganshanbian (Herba Hyperici Attenuati) effect the expression of CaL-alpha1C and K(ATP)-Kir6.1 mRNA of the myocardial cell membrane in myocardial ischemia-reperfusion arrhythmia rats.
abstract: OBJECTIVE
  To observe the impact of total flavonoids from Ganshanbian (Herba Hyperici Attenuati) on the expression of vascular smooth muscle membrane L-type calcium channel alpha1 C subunit (CaL-alpha1C) and ATP-sensitive K+ channel (K(ATP))-Kir6.1 mRNA, and explore the mechanisms of the antiarrhythmic effect of Ganshanbian (Herba Hyperici Attenuati) total flavonoids.
  
  
  METHODS
  The treatment group was fed total flavonoids from Ganshanbian (Herba Hyperici Attenuati) for 7 days by gavage with 100 mg x kg(-1) x d(-1). The blank control group and model control group were given the same amount of normal saline for 7 d. Arrhythmias were induced by performing a myocardial ischemia-reperfusion and electrocardiogram was observed. Reverse transcription-polymerase chain reaction was used to detect the expression of CaL-alpha 1C and K(ATP)-Kir6.1 mRNA in the myocardial cell membrane of all groups of rats.
  
  
  RESULTS
  Total flavonoids from Ganshanbian (Herba Hyperici Attenuati) can delay the appearance of myocardial ischemia reperfusion arrhythmias, shorten the duration of myocardial ischemia reperfusion arrhythmias, reduce heart rate, reduce cell membrane expression of CaL-a1C mRNA and enhance the expression of K(ATP)-Kir6.1 mRNA in myocardial ischemia-reperfusion arrhythmic rats.
  
  
  CONCLUSIONS
  Total flavonoids from Ganshanbian (Herba Hyperici Attenuati) can alleviate arrhythmias by affecting the expression of L-type calcium channels and ATP-sensitive K+ channels.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Ma
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanyu
authors.initials: Y
authors.lastname: Gao
authors.forename: Yin
authors.initials: Y
authors.lastname: Fu
authors.forename: Ji
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000008:administration & dosage*; D001145:Arrhythmias, Cardiac / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D005419:Flavonoids / Q000008:administration & dosage*; D006801:Humans; D054086:KATP Channels / Q000235:genetics* / Q000378:metabolism; D008297:Male; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000889:Anti-Arrhythmia Agents; D020746:Calcium Channels, L-Type; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D054086:KATP Channels; C092520:uK-ATP-1 potassium channel