title: Experimental protection of diabetic mice against Lethal P. aeruginosa infection by bacteriophage.
abstract: The emergence of antibiotic-resistant bacterial strains has become a global crisis and is vulnerable for the exploration of alternative antibacterial therapies. The present study emphasizes the use of bacteriophage for the treatment of multidrug resistant P. aeruginosa. P. aeruginosa was used to induce septicemia in streptozotocin (STZ) induced diabetic and nondiabetic mice by intraperitoneal (i.p.) injection of 3 × 10(8) CFU, resulting in a fatal bacteremia within 48 hrs. A single i.p. injection of 3 × 10(9) PFU phage GNCP showed efficient protection in both diabetic (90%) and nondiabetic (100%) bacteremic mice. It was further noted that the protection rate was reduced in diabetic mice when phage GNCP was administered after 4 h and 6 h of lethal bacterial challenge. In contrast, nondiabetic bacteremic mice were rescued even when treatment was delayed up to 20 h after lethal bacterial challenge. Evaluation of results confirmed that a single intraperitoneal injection of the phage dose (3 × 10(9) PFU/mL) was more effective than the multiple doses of imipenem. These results uphold the efficacy of phage therapy against pernicious P. aeruginosa infections, especially in cases of immunocompromised host.
authors.affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
authors.forename: Nagaveni
authors.initials: N
authors.lastname: Shivshetty
authors.affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
authors.forename: Rajeshwari
authors.initials: R
authors.lastname: Hosamani
authors.affiliation: Luqman College of Pharmacy, Gulbarga, Karnataka 585101, India.
authors.forename: Liyakat
authors.initials: L
authors.lastname: Ahmed
authors.affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Oli
authors.affiliation: Luqman College of Pharmacy, Gulbarga, Karnataka 585101, India.
authors.forename: Syed
authors.initials: S
authors.lastname: Sannauallah
authors.affiliation: Department of Pathology, M. R. Medical College, Gulbarga, Karnataka 585104, India.
authors.forename: Shivshetty
authors.initials: S
authors.lastname: Sharanbassappa
authors.affiliation: Department of Neuromicrobiology, NIMHANS, Bangalore, Karnataka 560 029, India.
authors.forename: S A
authors.initials: SA
authors.lastname: Patil
authors.affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.
authors.forename: Chandrakanth R
authors.initials: CR
authors.lastname: Kelmani
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics* / Q000472:pathogenicity; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000628:therapy* / Q000821:virology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity / Q000821:virology; D018805:Sepsis / Q000382:microbiology / Q000473:pathology / Q000628:therapy* / Q000821:virology