title: Antibiotic susceptibility pattern of genital tract bacteria in pregnant women with preterm premature rupture of membranes in a resource-limited setting.
abstract: OBJECTIVE
  To identify microbes prevalent in the genital tract of pregnant women with preterm premature rupture of membranes (PPROM) and to assess the susceptibility of the microbial isolates to a range of antibiotics to determine appropriate antibiotics for treating cases of PPROM in resource-limited settings.
  
  
  METHODS
  A prospective cross-sectional study was undertaken involving women with (n=105) and without (n=105) a confirmed diagnosis of PPROM admitted to Nnamdi Azikiwe University Teaching Hospital, southeast Nigeria, between January 1, 2011, and April 30, 2013. Endocervical swabs were collected from all participants and examined microbiologically. Antibiotic sensitivity testing was performed using Kirby-Bauer disk diffusion.
  
  
  RESULTS
  Streptococcus spp., Staphylococcus aureus, and Escherichia coli were significantly more prevalent among women with PPROM than among those without PPROM (P<0.01). Among the antibiotics considered safe to use during pregnancy, the bacteria were most sensitive to ampicillin-sulbactam, cefixime, cefuroxime, and erythromycin.
  
  
  CONCLUSIONS
  For the first 48hours, women with PPROM should receive an intravenous dose combining ampicillin-sulbactam, cefixime, cefuroxime, or erythromycin with metronidazole followed by oral administration of the chosen antibiotic combination to complete a 7-day course.
authors.affiliation: Department of Obstetrics and Gynecology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria. Electronic address: georgel21@yahoo.com.
authors.forename: George U
authors.initials: GU
authors.lastname: Eleje
authors.affiliation: Department of Obstetrics and Gynecology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria.
authors.forename: Joseph I
authors.initials: JI
authors.lastname: Adinma
authors.affiliation: Department of Pharmacology and Therapeutics, College of Medicine, University of Nigeria, Enugu, Nigeria.
authors.forename: Samuel
authors.initials: S
authors.lastname: Ghasi
authors.affiliation: Department of Obstetrics and Gynecology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria.
authors.forename: Joseph I
authors.initials: JI
authors.lastname: Ikechebelu
authors.affiliation: Department of Obstetrics and Gynecology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria.
authors.forename: Anthony O
authors.initials: AO
authors.lastname: Igwegbe
authors.affiliation: Department of Obstetrics and Gynecology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria.
authors.forename: John E
authors.initials: JE
authors.lastname: Okonkwo
authors.affiliation: Department of Obstetrics and Gynecology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria.
authors.forename: Charles I
authors.initials: CI
authors.lastname: Okafor
authors.affiliation: Department of Obstetrics and Gynecology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria.
authors.forename: Chukwuemeka O
authors.initials: CO
authors.lastname: Ezeama
authors.affiliation: Department of Obstetrics and Gynecology, Anambra State University Teaching Hospital, Awka, Nigeria.
authors.forename: Ifeanyichukwu U
authors.initials: IU
authors.lastname: Ezebialu
authors.affiliation: Department of Parasitology and Entomology, Nnamdi Azikiwe University, Awka, Nigeria.
authors.forename: Chukwuanugo N
authors.initials: CN
authors.lastname: Ogbuagu
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D003430:Cross-Sectional Studies; D003906:Developing Countries; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000382:microbiology*; D005836:Genitalia, Female / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000517:prevention & control; D011446:Prospective Studies
chemical_list: D000900:Anti-Bacterial Agents
keywords: Aerobic cervical bacteriology; Antibiotic sensitivity testing; Antibiotic susceptibility; Female genital tract; Microbiology; Pregnant women; Preterm premature rupture of membranes (PPROM)