title: One-stop microfiber spinning and fabrication of a fibrous cell-encapsulated scaffold on a single microfluidic platform.
abstract: This paper provides a method for microscale fiber spinning and the in situ construction of a 3D fibrous scaffold on a single microfluidic platform. This platform was also used to fabricate a variety of fibrous scaffolds with diverse compositions without the use of complicated devices. We explored the potential utility of the fibrous scaffolds for tissue engineering applications by constructing a fibrous scaffold encapsulating primary hepatocytes. The cells in scaffold were cultured over seven days and maintained higher viability comparing with 3D alginate non-fibrous block. The main advantage of this platform is that the fibrous structure used to form a scaffold can be generated without damaging the mechanically weak alginate fibers or encapsulated cells because all procedures are performed in a single platform without the intervention of the operator. In addition, the proposed fibrous scaffold permitted high diffusion capability of molecules, which enabled better viability of encapsulated cells than non-fibrous scaffold even in massive cell culture.
authors.forename: D Y
authors.initials: DY
authors.lastname: Park
authors.forename: C H
authors.initials: CH
authors.lastname: Mun
authors.forename: E
authors.initials: E
authors.lastname: Kang
authors.forename: D Y
authors.initials: DY
authors.lastname: No
authors.forename: J
authors.initials: J
authors.lastname: Ju
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D004058:Diffusion; D004867:Equipment Design; D020723:Glucuronic Acid / Q000737:chemistry; D022781:Hepatocytes / Q000166:cytology; D006603:Hexuronic Acids / Q000737:chemistry; D008297:Male; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid