title: LC-MS/MS determination of colistin in Mueller-Hinton broth for in vitro pharmacodynamic studies.
abstract: A rapid and simple method was developed and validated for the determination of colistin A and B in Mueller-Hinton broth using LC-tandem MS. Both analyte and internal standard (IS) (polymyxin B1) were determined using ESI. The MS data were obtained via the selected reaction monitoring in positive-ion mode. A linear regression (weighted 1/concentration) was used to fit calibration curves over the concentration range of 0.0241-2.41 μg ml(-1) for colistin A and 0.0439-4.39 μg ml(-1) for colistin B. No interference peaks were found in the blank Mueller-Hinton broth tested. Inter- and intraday precision and accuracy were within 85-115% (coefficient of variation). Colistin was stable in the autosampler for at least 24 h at 4 °C and in Mueller-Hinton broth for at least 120 h at 35 °C. This assay has been successfully used to determine colistin A and B in Mueller-Hinton broth for in vitro pharmacodynamic model studies. Accurate determination of colistin in bacterial growth medium has a vital role in the studies examining dosage regimen of and bacterial resistance to colistin.
authors.affiliation: Institute of Antibiotics, Huashan Hospital, Fudan University and Key Laboratory of Clinical Pharmacology of Antibiotics, National Health and Family Planning Commission, Shanghai, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Institute of Antibiotics, Huashan Hospital, Fudan University and Key Laboratory of Clinical Pharmacology of Antibiotics, National Health and Family Planning Commission, Shanghai, China.
authors.forename: Yu-Ran
authors.initials: YR
authors.lastname: Cao
authors.affiliation: Institute of Antibiotics, Huashan Hospital, Fudan University and Key Laboratory of Clinical Pharmacology of Antibiotics, National Health and Family Planning Commission, Shanghai, China.
authors.forename: Bei-Ning
authors.initials: BN
authors.lastname: Guo
authors.affiliation: Institute of Antibiotics, Huashan Hospital, Fudan University and Key Laboratory of Clinical Pharmacology of Antibiotics, National Health and Family Planning Commission, Shanghai, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Wu
authors.affiliation: Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Antibiotics, Huashan Hospital, Fudan University and Key Laboratory of Clinical Pharmacology of Antibiotics, National Health and Family Planning Commission, Shanghai, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D003091:Colistin / Q000032:analysis*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods
chemical_list: D003470:Culture Media; D003091:Colistin