title: Identification of human eosinophils in whole blood by flow cytometry.
abstract: Identification of eosinophils in whole blood samples by flow cytometry is often problematic. There are usually only a low number and percentage of cells that may be detected, and it may be difficult to discriminate eosinophils from other granulocytes. Here, we propose a simple approach using the eosinophil's intrinsic autofluorescence properties, combined with detection of CCR3 expression, to reliably identify eosinophils in a mixed leukocyte population.
authors.affiliation: Pulmonary Research Group, University of Alberta, Edmonton, AB, Canada.
authors.forename: Caroline
authors.initials: C
authors.lastname: Ethier
authors.forename: Paige
authors.initials: P
authors.lastname: Lacy
authors.forename: Francis
authors.initials: F
authors.lastname: Davoine
mesh_terms: D004804:Eosinophils / Q000166:cytology*; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology; D006801:Humans