title: Maternal and fetal metabonomic alterations in prenatal nicotine exposure-induced rat intrauterine growth retardation.
abstract: Prenatal nicotine exposure causes adverse birth outcome. However, the corresponding metabonomic alterations and underlying mechanisms of nicotine-induced developmental toxicity remain unclear. The aims of this study were to characterize the metabolic alterations in biofluids in nicotine-induced intrauterine growth retardation (IUGR) rat model. In the present study, pregnant Wistar rats were intragastrically administered with different doses of nicotine (0.5, 1.0 and 2.0 mg/kg d) from gestational day (GD) 11-20. The metabolic profiles of the biofluids, including maternal plasma, fetal plasma and amniotic fluid, were analyzed using (1)H nuclear magnetic resonance (NMR)-based metabonomic techniques. Prenatal nicotine exposure caused noticeably lower body weights, higher IUGR rates of fetal rats, and elevated maternal and fetal corticosterone (CORT) levels compared to the controls. The correlation analysis among maternal, fetal serum CORT levels and fetal bodyweight suggested that the levels of maternal and fetal serum CORT presented a positive correlation (r=0.356, n=32, P<0.05), while there was a negative correlation between fetal (r=-0.639, n=32, P<0.01) and maternal (r=-0.530, n=32, P<0.01) serum CORT level and fetal bodyweight. The fetal metabonome alterations included the stimulation of lipogenesis and the decreased levels of glucose and amino acids. The maternal metabonome alterations involved the enhanced blood glucose levels, fatty acid oxygenolysis, proteolysis and amino acid accumulation. These results suggested that prenatal nicotine exposure is associated with an altered maternal and fetal metabonome, which may be related to maternal increased glucocorticoid level induced by nicotine.
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, China. Electronic address: jianghua.feng@xmu.edu.cn.
authors.forename: Jiang-hua
authors.initials: JH
authors.lastname: Feng
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Wuhan University, Wuhan 430071, China.
authors.forename: You-e
authors.initials: YE
authors.lastname: Yan
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Wuhan University, Wuhan 430071, China.
authors.forename: Gai
authors.initials: G
authors.lastname: Liang
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Wuhan University, Wuhan 430071, China.
authors.forename: Yan-song
authors.initials: YS
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Wuhan University, Wuhan 430071, China.
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Wuhan University, Wuhan 430071, China.
authors.forename: Ben-jian
authors.initials: BJ
authors.lastname: Zhang
authors.affiliation: Research Center of Food and Drug Evaluation, Wuhan University, Wuhan 430071, China.
authors.forename: Liao-bin
authors.initials: LB
authors.lastname: Chen
authors.affiliation: Research Center of Food and Drug Evaluation, Wuhan University, Wuhan 430071, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.affiliation: Research Center of Food and Drug Evaluation, Wuhan University, Wuhan 430071, China.
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: He
authors.affiliation: Department of Pharmacology, School of Basic Medical Science, Wuhan University, Wuhan 430071, China; Research Center of Food and Drug Evaluation, Wuhan University, Wuhan 430071, China. Electronic address: wanghui19@whu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000653:Amniotic Fluid / Q000737:chemistry; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005317:Fetal Growth Retardation / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D020567:Fetal Weight / Q000187:drug effects; D005333:Fetus / Q000187:drug effects* / Q000473:pathology; D005865:Gestational Age; D050155:Lipogenesis; D008297:Male; D018811:Maternal Exposure; D055432:Metabolomics; D009538:Nicotine / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D005227:Fatty Acids; D009538:Nicotine; D003345:Corticosterone
keywords: Biomarker; Intrauterine growth retardation; Metabonomics; Nicotine; Nuclear magnetic resonance