title: Studies on quantitative determination of total alkaloids and berberine in five origins of crude medicine "Sankezhen".
abstract: The roots of Berberis plants are widely used as a traditional Chinese medicine called "Sankezhen", having the activities of antibacterial and anti-inflammatory, and the ingredients are alkaloids. This work aims to study and compare the total alkaloids and individual alkaloid (berberine) contents in roots and stems from five origins of Berberis plants (Berberis soulieana Schneid., B. henryana Schneid., B. triacanthophora Fedde, B. gagnepainii Schneid. and B. bergmanniae Schneid.) and provides some references for resource and quality evaluation of the medicine. Acid dye colorimetry and high-performance liquid chromatography were used in the determination. The results showed that the contents for the total alkaloids in root and stem samples were in the range of 1.60-4.72% and 0.76-2.70%, while those of the berberine were 0.70-2.92% and 0.23-1.07%. With higher contents of the total alkaloids and berberine, the roots of B. soulieana, B. gagnepainii and B. bergmanniae were good sources of "Sankezhen". Meanwhile, the contents were also high in stems of the three plants, indicating that the stems were likely to be alternative sources of "Sankezhen" after further research. As the results of precision, stability and recovery tests shown, the methods were simple, rapid and reliable, and provided valuable basis for quality evaluation and new resource investigation of "Sankezhen".
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan 430074, China.
authors.forename: Luyang
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan 430074, China.
authors.forename: Weifang
authors.initials: W
authors.lastname: Long
authors.affiliation: Luojia College, Wuhan University, Wuhan 430064, China.
authors.forename: Xiangluan
authors.initials: X
authors.lastname: Wan
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan 430074, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Ding
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan 430074, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan 430074, China Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Wuhan 430074, China wandr666@163.com.
authors.forename: Dingrong
authors.initials: D
authors.lastname: Wan
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry; D001599:Berberine / Q000032:analysis* / Q000737:chemistry; D028142:Berberis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D016014:Linear Models; D015203:Reproducibility of Results
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D001599:Berberine