title: Association of levels of antibodies from patients with inflammatory bowel disease with extracellular proteins of food and probiotic bacteria.
abstract: Inflammatory bowel disease (IBD) is an autoimmune disease characterized by a chronic inflammation of the gastrointestinal tract mucosa and is related to an abnormal immune response to commensal bacteria. Our aim of the present work has been to explore the levels of antibodies (IgG and IgA) raised against extracellular proteins produced by LAB and its association with IBD. We analyzed, by Western-blot and ELISA, the presence of serum antibodies (IgA and IgG) developed against extracellular protein fractions produced by different food bacteria from the genera Bifidobacterium and Lactobacillus. We used a sera collection consisting of healthy individuals (HC, n = 50), Crohn's disease patients (CD, n = 37), and ulcerative colitis patients (UC, n = 15). Levels of IgA antibodies developed against a cell-wall hydrolase from Lactobacillus casei subsp. rhamnosus GG (CWH) were significantly higher in the IBD group (P < 0.002; n = 52). The specificity of our measurements was confirmed by measuring IgA antibodies developed against the CWH peptide 365-VNTSNQTAAVSAS-377. IBD patients appeared to have different immune response to food bacteria. This paper sets the basis for developing systems for early detection of IBD, based on the association of high levels of antibodies developed against extracellular proteins from food and probiotic bacteria.
authors.affiliation: Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, Villaviciosa, 33300 Asturias, Spain.
authors.forename: Arancha
authors.initials: A
authors.lastname: Hevia
authors.affiliation: Department of Functional Biology, Immunology Area, University of Oviedo, C/Julián Clavería s/n, Oviedo, 33006 Asturias, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: López
authors.affiliation: Department of Functional Biology, Immunology Area, University of Oviedo, C/Julián Clavería s/n, Oviedo, 33006 Asturias, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Suárez
authors.affiliation: UMR 5248 CBMN CNRS-Université Bordeaux 1-ENITAB, Laboratoire de Microbiologie et Biochimie Appliquée, 1 Cours du Général de Gaulle, 33175 Gradignan Cedex, France.
authors.forename: Claudine
authors.initials: C
authors.lastname: Jacquot
authors.affiliation: UMR 5248 CBMN CNRS-Université Bordeaux 1-ENITAB, Laboratoire de Microbiologie et Biochimie Appliquée, 1 Cours du Général de Gaulle, 33175 Gradignan Cedex, France.
authors.forename: María C
authors.initials: MC
authors.lastname: Urdaci
authors.affiliation: Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, Villaviciosa, 33300 Asturias, Spain.
authors.forename: Abelardo
authors.initials: A
authors.lastname: Margolles
authors.affiliation: Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Food Science and Technology Faculty, University of Vigo, Ourense Campus, 32004 Ourense, Spain.
authors.forename: Borja
authors.initials: B
authors.lastname: Sánchez
mesh_terms: D000328:Adult; D000906:Antibodies / Q000097:blood / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification; D001644:Bifidobacterium / Q000276:immunology / Q000472:pathogenicity; D003093:Colitis, Ulcerative / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D003424:Crohn Disease / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D005260:Female; D005516:Food Microbiology; D006801:Humans; D007111:Immunity, Cellular; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000276:immunology* / Q000473:pathology; D007778:Lactobacillus / Q000276:immunology / Q000472:pathogenicity; D007780:Lacticaseibacillus casei / Q000276:immunology* / Q000472:pathogenicity; D008297:Male; D008875:Middle Aged; D019936:Probiotics
chemical_list: D000906:Antibodies; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G