title: Amino-polyvinyl alcohol coated superparamagnetic iron oxide nanoparticles are suitable for monitoring of human mesenchymal stromal cells in vivo.
abstract: Mesenchymal stromal cells (MSCs) are promising candidates in regenerative cell-therapies. However, optimizing their number and route of delivery remains a critical issue, which can be addressed by monitoring the MSCs' bio-distribution in vivo using super-paramagnetic iron-oxide nanoparticles (SPIONs). In this study, amino-polyvinyl alcohol coated (A-PVA) SPIONs are introduced for cell-labeling and visualization by magnetic resonance imaging (MRI) of human MSCs. Size and surface charge of A-PVA-SPIONs differ depending on their solvent. Under MSC-labeling conditions, A-PVA-SPIONs have a hydrodynamic diameter of 42 ± 2 nm and a negative Zeta potential of 25 ± 5 mV, which enable efficient internalization by MSCs without the need to use transfection agents. Transmission X-ray microscopy localizes A-PVA-SPIONs in intracellular vesicles and as cytosolic single particles. After identifying non-interfering cell-assays and determining the delivered and cellular dose, in addition to the administered dose, A-PVA-SPIONs are found to be non-toxic to MSCs and non-destructive towards their multi-lineage differentiation potential. Surprisingly, MSC migration is increased. In MRI, A-PVA-SPION-labeled MSCs are successfully visualized in vitro and in vivo. In conclusion, A-PVA-SPIONs have no unfavorable influences on MSCs, although it becomes evident how sensitive their functional behavior is towards SPION-labeling. And A-PVA-SPIONs allow MSC-monitoring in vivo.
authors.affiliation: Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353, Berlin, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Schulze
authors.forename: Anke
authors.initials: A
authors.lastname: Dienelt
authors.forename: Sven
authors.initials: S
authors.lastname: Geissler
authors.forename: Paul
authors.initials: P
authors.lastname: Zaslansky
authors.forename: Janosch
authors.initials: J
authors.lastname: Schoon
authors.forename: Katja
authors.initials: K
authors.lastname: Henzler
authors.forename: Peter
authors.initials: P
authors.lastname: Guttmann
authors.forename: Azza
authors.initials: A
authors.lastname: Gramoun
authors.forename: Lindsey A
authors.initials: LA
authors.lastname: Crowe
authors.forename: Lionel
authors.initials: L
authors.lastname: Maurizi
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Vallée
authors.forename: Heinrich
authors.initials: H
authors.lastname: Hofmann
authors.forename: Georg N
authors.initials: GN
authors.lastname: Duda
authors.forename: Andrea
authors.initials: A
authors.lastname: Ode
mesh_terms: D000368:Aged; D000818:Animals; D002454:Cell Differentiation; D058948:Cell Tracking / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry; D003911:Dextrans / Q000138:chemical synthesis / Q000737:chemistry*; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008875:Middle Aged; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D011142:Polyvinyl Alcohol; C065507:ferumoxides
keywords: cell based therapies; magnetic resonance imaging; mesenchymal stromal cells; polyvinylalcohol; superparamagnetic iron oxide nanoparticles