title: Transcriptional activation of OCT4 by the ETS transcription factor PEA3 in NCCIT human embryonic carcinoma cells.
abstract: We examined the molecular mechanism of OCT4 gene regulation by polyomavirus enhancer activator 3 (PEA3) in NCCIT cells. Endogenous PEA3 and OCT4 were significantly elevated in undifferentiated cells and reduced upon differentiation. PEA3 knockdown led to a reduction in OCT4 levels. OCT4 promoter activity was significantly up-regulated by dose-dependent PEA3 overexpression. Deletion and site-directed mutagenesis of the OCT4 promoter revealed a putative binding site within the conserved region 2 (CR2). PEA3 interacted with the binding element within CR2 in NCCIT cells. This study reveals the molecular details of the mechanism by which the oncogenic factor PEA3 regulates OCT4 gene expression as a transcriptional activator.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam-si, Gyeonggi-Do, South Korea.
authors.forename: Sung-Won
authors.initials: SW
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam-si, Gyeonggi-Do, South Korea.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Do
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam-si, Gyeonggi-Do, South Korea.
authors.forename: Woo Tae
authors.initials: WT
authors.lastname: Ha
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam-si, Gyeonggi-Do, South Korea.
authors.forename: Mi-Hee
authors.initials: MH
authors.lastname: Han
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam-si, Gyeonggi-Do, South Korea.
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
authors.affiliation: Department of Animal & Food Bioscience, College of Natural Science, Konkuk University, Chungju, Chungbuk, South Korea.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, South Korea.
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam-si, Gyeonggi-Do, South Korea. Electronic address: jaehwan_k@cha.ac.kr.
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D047108:Embryonic Development; D006801:Humans; D014411:Neoplastic Stem Cells / Q000473:pathology; D050814:Octamer Transcription Factor-3 / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D018239:Seminoma / Q000473:pathology; D017384:Sequence Deletion; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D014157:Transcription Factors; C065788:transcription factor PEA3
keywords: ETS family transcription factor; Embryonic carcinoma; NCCIT; OCT4 promoter; Polyomavirus enhancer activator 3