title: Azumiobodo hoyamushi, the kinetoplastid causing soft tunic syndrome in ascidians, may invade through the siphon wall.
abstract: The infectious kinetoplastid Azumiobodo hoyamushi causes 'soft tunic syndrome', a serious problem in aquaculture of the edible ascidian Halocynthia roretzi. Infection tests using diseased tunics demonstrated that juvenile (0.8 yr old) individuals never developed soft tunic syndrome, but all individuals in the other age groups (1.8, 2.8, and 3.8 yr old) showed the disease symptoms. In the infection tests, tunic softening was first observed at the tunic around siphons. Based on ultrastructural observation of the inner wall of the branchial siphon, the tunic lining the inner wall in juveniles (0.5 yr old) was completely covered with cuticle, which had a dense structure to prevent bacterial and protist invasion. In contrast, the tunic was often partly damaged and not covered with cuticle in healthy adults (≥2.5 yr old). The damaged tunic in the siphon wall could be an entrance for A. hoyamushi into the tunic of adult hosts.
authors.affiliation: Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.
authors.forename: Euichi
authors.initials: E
authors.lastname: Hirose
authors.forename: Akira
authors.initials: A
authors.lastname: Kumagai
authors.forename: Akatsuki
authors.initials: A
authors.lastname: Nawata
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D006790:Host-Parasite Interactions; D034582:Integumentary System; D016830:Kinetoplastida / Q000502:physiology*; D014561:Urochordata / Q000469:parasitology*