title: Regulation of myeloid ecotropic viral integration site 1 and its expression in normal and abnormal endometrium.
abstract: OBJECTIVE
  To identify the expression profile and sex steroid regulation pattern of myeloid ecotropic viral integration site 1 (MEIS1) in endometrium.
  
  
  METHODS
  Molecular studies in human and animal tissue.
  
  
  METHODS
  Reproductive medicine center of a university hospital.
  
  
  METHODS
  Women with normal menstrual cycles for male infertility and female infertility with endometriosis. Sexually mature female mice (Kunming White strain).
  
  
  METHODS
  Primary cultured endometrial stromal cells, Ishikawa cells, and oophorectomized mice were treated with sex steroid.
  
  
  METHODS
  MEIS1 expression in the human endometrium during the menstrual cycle, mouse uterus during the peri-implantation period of pregnancy, and eutopic endometrium from patients with endometriosis was analyzed by immunohistochemistry staining and western blot. In addition, MEIS1 expression in response to sex steroid was examined both in vitro and in vivo by immunohistochemistry staining and western blot.
  
  
  RESULTS
  MEIS1 expression was markedly increased in endometrium during the implantation period, and in decidualizing stromal cells in human endometrium and murine uterus. Steroid hormones increased MEIS1 expression in primary cultured endometrial stromal cells, Ishikawa cells, and endometrium of oophorectomized mice. The effects of estrogen and progesterone were more marked in oophorectomized mice and were additive. MEIS1 expression was significantly lower in eutopic endometrium compared with normal endometrium in the midsecretory stage.
  
  
  CONCLUSIONS
  MEIS1 is likely a key mediator between sex steroid and genes for uterine receptivity. Diminished endometrium MEIS1 expression may contribute to implantation failure in endometriosis.
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Repubic of China; Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Repubic of China.
authors.forename: Linli
authors.initials: L
authors.lastname: Hu
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Repubic of China; Reproductive Medicine Center, Guangzhou General Hospital, Guangzhou Military Command of the Chinese People's Liberation Army, Guangzhou, People's Repubic of China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Li
authors.affiliation: Waisman Center, University of Wisconsin, Madison, Wisconsin.
authors.forename: CindyTzu-Ling
authors.initials: CL
authors.lastname: Huang
authors.affiliation: Waisman Center, University of Wisconsin, Madison, Wisconsin.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Repubic of China.
authors.forename: Guijin
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Repubic of China. Electronic address: qk1129@tjh.tjmu.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003656:Decidua / Q000378:metabolism; D010064:Embryo Implantation / Q000235:genetics*; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D000075064:Myeloid Ecotropic Viral Integration Site 1 Protein; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D011247:Pregnancy; D014591:Uterine Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D012739:Gonadal Steroid Hormones; D018398:Homeodomain Proteins; C000616391:MEIS1 protein, human; C000616392:Meis1 protein, mouse; D000075064:Myeloid Ecotropic Viral Integration Site 1 Protein; D009363:Neoplasm Proteins
keywords: MEIS1; endometriosis; implantation; steroids