title: Effect of dietary n - 3 polyunsaturated fatty acids on oxidant/antioxidant status in macrosomic offspring of diabetic rats.
abstract: The aim of this work was to determine the effect of dietary n - 3 PUFA on oxidant/antioxidant status, in vitro very low and low density lipoprotein (VLDL-LDL), and VLDL-LDL-fatty acid composition in macrosomic pups of diabetic mothers. We hypothesized that n - 3 PUFA would improve oxidative stress in macrosomia. Diabetes was induced in female Wistar rats fed with the ISIO diet (control) or with the EPAX diet (enriched in n - 3 PUFAs), by streptozotocin. The macrosomic pups were killed at birth (day 0) and at adulthood (day 90). Lipid parameters and VLDL-LDL-fatty acid composition were investigated. The oxidant/antioxidant status was determined by measuring plasma oxygen radical absorbance capacity (ORAC), hydroperoxides, carbonyl proteins, and VLDL-LDL oxidation. Macrosomic rats of ISIO fed diabetic mothers showed an increase in plasma and VLDL-LDL-triglycerides and VLDL-LDL-cholesterol levels and altered VLDL-LDL-fatty acid composition. Plasma ORAC was low with high hydroperoxide and carbonyl protein levels. The in vitro oxidizability of VLDL-LDL was enhanced in these macrosomic rats. The EPAX diet corrected lipid parameters and improved oxidant/antioxidant status but increased VLDL-LDL susceptibility to oxidation. Macrosomia is associated with lipid abnormalities and oxidative stress. n - 3 PUFA exerts favorable effects on lipid metabolism and on the oxidant/antioxidant status of macrosomic rats. However, there are no evident effects on VLDL-LDL oxidation.
authors.affiliation: Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou Bekr Belkaid, BP 119, 13000 Tlemcen, Algeria.
authors.forename: B
authors.initials: B
authors.lastname: Guermouche
authors.affiliation: Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou Bekr Belkaid, BP 119, 13000 Tlemcen, Algeria.
authors.forename: N A
authors.initials: NA
authors.lastname: Soulimane-Mokhtari
authors.affiliation: Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou Bekr Belkaid, BP 119, 13000 Tlemcen, Algeria.
authors.forename: S
authors.initials: S
authors.lastname: Bouanane
authors.affiliation: Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Abou Bekr Belkaid, BP 119, 13000 Tlemcen, Algeria.
authors.forename: H
authors.initials: H
authors.lastname: Merzouk
authors.affiliation: Department of Technical Sciences, Faculty of Engineering, University of Abou Bekr Belkaid, 13000 Tlemcen, Algeria.
authors.forename: S
authors.initials: S
authors.lastname: Merzouk
authors.affiliation: INSERM UMR 866, "Lipids Nutrition Cancer," Faculty of Life, Earth and Environment Sciences, University of Burgundy, 21000 Dijon, France.
authors.forename: M
authors.initials: M
authors.lastname: Narce
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental; D004041:Dietary Fats / Q000494:pharmacology*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005260:Female; D005320:Fetal Macrosomia / Q000097:blood* / Q000178:diet therapy; D008297:Male; D016877:Oxidants / Q000097:blood; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D016877:Oxidants