title: Floral glycerolipid profiles in homeotic mutants of Arabidopsis thaliana.
abstract: Flowers have distinct glycerolipid composition, yet its floral organ-specific profile remains elusive in Arabidopsis whose flowers are too tiny to dissect different floral organs. Here, we employed known floral homeotic mutants agamous-1 (ag-1) and apetala3-3 (ap3-3) to facilitate sample preparation enriched in different floral organs. The result of analysis on different polar glycerolipid classes and their fatty acid composition demonstrated that flowers of ap3-3 and ag-1 have distinct glycerolipid composition from that of wild type. Moreover, distinct set of glycerolipid biosynthetic genes is expressed in these mutants by qRT-PCR gene expression analysis. These data suggest that glycerolipid profile is distinct among different floral organs of Arabidopsis thaliana.
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, 128 sec.2, Academia Rd., Nankang, Taipei 11529, Taiwan. Electronic address: nakamura@gate.sinica.edu.tw.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, 128 sec.2, Academia Rd., Nankang, Taipei 11529, Taiwan.
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, 128 sec.2, Academia Rd., Nankang, Taipei 11529, Taiwan.
authors.forename: Ying-Chen
authors.initials: YC
authors.lastname: Lin
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D035264:Flowers / Q000378:metabolism*; D020869:Gene Expression Profiling; D005801:Genes, Homeobox; D017343:Genes, Plant; D006017:Glycolipids / Q000378:metabolism*; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D006017:Glycolipids
keywords: Arabidopsis; Floral organs; Glycerolipids; Homeotic mutants