title: Influence of ribosomal protein L39-L in the drug resistance mechanisms of lacrimal gland adenoid cystic carcinoma cells.
abstract: BACKGROUND
  Cancer constitutes a key pressure on public health regardless of the economy state in different countries. As a kind of highly malignant epithelial tumor, lacrimal gland adenoid cystic carcinoma can occur in any part of the body, such as salivary gland, submandibular gland, trachea, lung, breast, skin and lacrimal gland. Chemotherapy is one of the key treatment techniques, but drug resistance, especially MDR, seriously blunts its effects. As an element of the 60S large ribosomal subunit, the ribosomal protein L39-L gene appears to be documented specifically in the human testis and many human cancer samples of different origins.
  
  
  METHODS
  Total RNA of cultured drug-resistant and susceptible lacrimal gland adenoid cystic carcinoma cells was seperated, and real time quantitative RT-PCR were used to reveal transcription differences between amycin resistant and susceptible strains of lacrimal gland adenoid cystic carcinoma cells. Viability assays were used to present the amycin resistance difference in a RPL39-L transfected lacrimal gland adenoid cystic carcinoma cell line as compared to control vector and null-transfected lacrimal gland adenoid cystic carcinoma cell lines.
  
  
  RESULTS
  The ribosomal protein L39-L transcription level was 6.5-fold higher in the drug-resistant human lacrimal gland adenoid cystic carcinoma cell line than in the susceptible cell line by quantitative RT-PCR analysis. The ribosomal protein L39-L transfected cells revealed enhanced drug resistance compared to plasmid vector-transfected or null-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation.
  
  
  CONCLUSIONS
  The ribosomal protein L39-L gene could possibly have influence on the drug resistance mechanism of lacrimal gland adenoid cystic carcinoma cells.
authors.affiliation: Department of Ophthalmology, Jining First People's Hospital, Jining, China E-mail : 1392144@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ye
authors.forename: Shao-Feng
authors.initials: SF
authors.lastname: Ding
authors.forename: Zhi-An
authors.initials: ZA
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Tan
mesh_terms: D015153:Blotting, Western; D003528:Carcinoma, Adenoid Cystic / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D005134:Eye Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D007766:Lacrimal Apparatus Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D006146:Guanidines; D012333:RNA, Messenger; C471571:RPL39L protein, human; D012269:Ribosomal Proteins; C065212:amycin A