title: Eosinophils and respiratory virus infection: a dual-standard curve qRT-PCR-based method for determining virus recovery from mouse lung tissue.
abstract: Several lines of investigation have indicated a role for eosinophilic leukocytes in limiting virus infectivity and promoting virion clearance. We have established a respiratory virus infection model with pneumonia virus of mice (PVM; family Paramyxoviridae), a natural mouse pathogen that replicates the more severe forms of human disease elicited by the phylogenetically related respiratory syncytial virus (RSV). In this chapter, we present a rapid and highly reproducible dual-standard curve qRT-PCR based method for quantitative detection of PVM replication in mouse lung tissue. We have used this assay to evaluate eosinophil-mediated antiviral host defense in mouse models of cytokine and antigen-driven eosinophilic inflammation.
authors.affiliation: Inflammation Immunobiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, 20892, Bethesda, MD, USA.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Percopo
authors.forename: Kimberly D
authors.initials: KD
authors.lastname: Dyer
authors.forename: Kendal A
authors.initials: KA
authors.lastname: Karpe
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Domachowske
authors.forename: Helene F
authors.initials: HF
authors.lastname: Rosenberg
mesh_terms: D000818:Animals; D004804:Eosinophils / Q000166:cytology* / Q000502:physiology; D008168:Lung / Q000276:immunology* / Q000821:virology*; D051379:Mice; D016133:Polymerase Chain Reaction; D012136:Respiratory Syncytial Viruses / Q000276:immunology / Q000302:isolation & purification*