title: The effect of dibenzo-p-dioxin- and dibenzofuran-contaminated soil on the earthworm Eisenia andrei.
abstract: Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) belong to the group of persistent organic pollutants, highly toxic environmental pollutants that include hydrophobic compounds with the tendency to bioaccumulate. Earthworms (Eisenia andrei) were exposed to PCDD/Fs-contaminated soil, and changes in their lipophilic structures and the gene expression of their defense molecules were followed. Damage to the intestinal wall and adjacent chloragogenous tissue was observed. Further, the up-regulation of the expression of several genes was detected. On the basis of these results, the mechanism of the impact of PCDD/Fs on earthworms has been proposed. Dioxins that accumulate in the lipophilic structures cause an increase in reactive oxidative species that triggers oxidative stress followed by the gene expression of two molecules that play a role in protection against oxidant toxicity, calreticulin (CRT) and Hsp70. Moreover, the effect of microbial biomass on the expression of coelomic cytolytic factor (CCF), a pattern recognition receptor, was also observed.
authors.affiliation: Department of Immunology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Vídeňská 1083, 142 20, Praha 4, Czech Republic. Electronic address: r.roubalova@biomed.cas.cz.
authors.forename: Radka
authors.initials: R
authors.lastname: Roubalová
authors.affiliation: Department of Immunology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Vídeňská 1083, 142 20, Praha 4, Czech Republic. Electronic address: dvorak@biomed.cas.cz.
authors.forename: Jiří
authors.initials: J
authors.lastname: Dvořák
authors.affiliation: Department of Immunology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Vídeňská 1083, 142 20, Praha 4, Czech Republic. Electronic address: kohler@biomed.cas.cz.
authors.forename: Petra
authors.initials: P
authors.lastname: Procházková
authors.affiliation: Biology Centre of the Academy of Sciences of the Czech Republic, v. v. i., Institute of Soil Biology, Na Sádkách 7, 370 05, České Budějovice, Czech Republic. Electronic address: danael@upb.cas.cz.
authors.forename: Dana
authors.initials: D
authors.lastname: Elhottová
authors.affiliation: Department of Immunology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Vídeňská 1083, 142 20, Praha 4, Czech Republic. Electronic address: rossmann@biomed.cas.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Rossmann
authors.affiliation: Department of Immunology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Vídeňská 1083, 142 20, Praha 4, Czech Republic. Electronic address: skanta@biomed.cas.cz.
authors.forename: František
authors.initials: F
authors.lastname: Škanta
authors.affiliation: Department of Immunology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Vídeňská 1083, 142 20, Praha 4, Czech Republic. Electronic address: mbilej@biomed.cas.cz.
authors.forename: Martin
authors.initials: M
authors.lastname: Bilej
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000032:analysis / Q000378:metabolism*; D004147:Dioxins / Q000032:analysis / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D009835:Oligochaeta / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: D001572:Benzofurans; D004147:Dioxins; D012987:Soil; D012989:Soil Pollutants; C023614:dibenzofuran; C020887:dibenzo(1,4)dioxin
keywords: CCF; Calreticulin; Chloragogenous tissue; Eisenia andrei; Hsp70; PCDD/Fs; ROS