title: Application of enrofloxacin and orbifloxacin disks approved in Japan for susceptibility testing of representative veterinary respiratory pathogens.
abstract: In this study, susceptibilities of Pasteurella multocida, Mannheimia haemolytica and Actinobacillus pleuropneumoniae to enrofloxacin and orbifloxacin were tested using an agar diffusion method with the commercial disks and a broth microdilution method. Good correlation between the 2 methods for enrofloxacin and orbifloxacin was observed for P. multocida (r = -0.743 and -0.818, respectively), M. haemolytica (r = -0.739 and -0.800, respectively) and A. pleuropneumoniae (r = -0.785 and -0.809, respectively). Based on the Clinical and Laboratory Standards Institute interpretive criteria for enrofloxacin, high-level categorical agreement between the 2 methods was found for P. multocida (97.9%), M. haemolytica (93.8%) and A. pleuropneumoniae (92.0%). Our findings indicate that the tested commercial disks can be applied for susceptibility testing of veterinary respiratory pathogens.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University, 101 Minami 4-chome, Koyama, Tottori 680-8533, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Harada
authors.forename: Masaru
authors.initials: M
authors.lastname: Usui
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Asai
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000494:pharmacology*; D007564:Japan; D016978:Mannheimia haemolytica / Q000187:drug effects*; D016979:Pasteurella multocida / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin; C095163:orbifloxacin