title: Novel dynamic culture system to support initiation of primordial follicle growth in prepubertal mouse ovaries.
abstract: OBJECTIVE
  To evaluate the impact of dynamic in vitro culture on initiation of early follicular growth in prepubertal mouse ovaries.
  
  
  METHODS
  Ovaries from 8-day-old BALB/c mice were cultured either in a dynamic system (n=28) or in a static system (n=20) for 4 days. Uncultured 8-day-old (n=9) or 12-day-old (n=17) ovaries served as baseline or in vivo controls, respectively.
  
  
  METHODS
  Academic research center.
  
  
  METHODS
  Newborn female BALB/c mice (n=37).
  
  
  METHODS
  None.
  
  
  METHODS
  Histologic follicle classification and counting and assessment of follicular viability via immunofluorescent staining.
  
  
  RESULTS
  The percentage of secondary follicles after dynamic culture was identical to the 12-day-old in vivo control. In contrast, after static culture ovaries showed a significantly higher percentage of secondary follicles. For immunofluorescent viability assessment 6.78 follicles per ovary could be isolated after dynamic culture, whereas only 3.8 follicles per ovary could be isolated after static culture.
  
  
  CONCLUSIONS
  Dynamic in vitro culture supports physiologic follicular growth initiation, comparable to that observed in vivo. In contrast, accelerated follicular growth was observed after static culture. These findings add additional evidence to the idea that dynamic culture might be a beneficial first step to initiate follicle growth in vitro within the context of fertility preservation.
authors.affiliation: Department of Gynecological Endocrinology and Reproductive Medicine, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Winkler-Crepaz
authors.affiliation: Department of Gynecological Endocrinology and Reproductive Medicine, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Verena
authors.initials: V
authors.lastname: Nederegger
authors.affiliation: Department of Gynecological Endocrinology and Reproductive Medicine, Innsbruck Medical University, Innsbruck, Austria; Department of Obstetrics and Gynecology, Gadjah Mada University, Yogyakarta, Indonesia.
authors.forename: Sarrah
authors.initials: S
authors.lastname: Ayuandari
authors.affiliation: Department of Gynecological Endocrinology and Reproductive Medicine, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Doris
authors.initials: D
authors.lastname: Rosenfellner
authors.affiliation: Department of Gynecological Endocrinology and Reproductive Medicine, Innsbruck Medical University, Innsbruck, Austria; Mitera IVF, Mitera Assisted Reproduction Unit, Athens, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Zervomanolakis
authors.affiliation: Department of Gynecological Endocrinology and Reproductive Medicine, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Susanne
authors.initials: S
authors.lastname: Hofer
authors.affiliation: Department of Gynecological Endocrinology and Reproductive Medicine, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Ludwig
authors.initials: L
authors.lastname: Wildt
authors.affiliation: Department of Gynecological Endocrinology and Reproductive Medicine, Innsbruck Medical University, Innsbruck, Austria. Electronic address: stephanie.ziehr@i-med.ac.at.
authors.forename: Stephanie C
authors.initials: SC
authors.lastname: Ziehr
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology*; D012741:Sexual Maturation
keywords: Dynamic in vitro culture; female fertility preservation; ovarian tissue culture