title: Hydrogen sulfide reduces kidney injury due to urinary-derived sepsis by inhibiting NF-κB expression, decreasing TNF-α levels and increasing IL-10 levels.
abstract: The present study aimed to investigate the effect of hydrogen sulfide (H2S) on kidney injury induced by urinary-derived sepsis. Rabbits were randomly divided into control, sham, sepsis, NaHS 2.8 μmol/kg and NaHS 8.4 μmol/kg groups, with six rabbits in each group. Upper urinary tract obstruction and acute infection was induced to establish the sepsis model. Blood was collected to carry out a white blood cell (WBC) count, and creatinine (Cr) and blood urea nitrogen (BUN) analysis. Morphological changes were observed by hematoxylin and eosin (H&E) staining and transmission electron microscopy. Immunohistochemical staining was used to detect the expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-10 and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB). Cystathionine-γ-lyase (CSE) activity was measured by the spectrophotometric methylene blue method and the blood H2S concentration was measured by deproteinization. WBC, Cr and BUN levels were significantly elevated in the sepsis group compared with those in the control group (P<0.05). Following treatment with NaHS, the WBC, Cr and BUN levels were significantly decreased in the NaHS groups compared with those in the sepsis group (P<0.05). The pathological features of kidney injury were also alleviated by NaHS. In the sepsis group, the levels of TNF-α, IL-10 and NF-κB were significantly increased compared with those in the control group (P<0.05). In the NaHS groups, the TNF-α and NF-κB levels were significantly reduced whereas the IL-10 level was significantly increased compared with the respective levels in the sepsis group (P<0.05). The H2S concentration was significantly decreased in the sepsis group and this reduction was attenuated in the NaHS groups (P<0.05). Furthermore, the NaHS 8.4 μmol/kg dose revealed a more potent effect than the NaHS 2.8 μmol/kg dose. Thus, exogenous H2S reduced kidney injury from urinary-derived sepsis by decreasing the levels of NF-κB and TNF-α, and increasing the level of IL-10.
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Xian
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Wujun
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Suqin
authors.initials: S
authors.lastname: Quan
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Long
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Neng
authors.initials: N
authors.lastname: Zhu
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Huang
authors.initials: H
authors.lastname: Xie
authors.affiliation: Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Luo
keywords: IL-10; NF-κB; TNF-α; hydrogen sulfide; kidney injury; urinary-derived sepsis