title: Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants.
abstract: Virus induced gene silencing (VIGS) is an effective technology for gene function analysis in plants. We assessed the VIGS effectiveness in Nicotiana benthamiana and citrus plants of different Citrus leaf blotch virus (CLBV)-based vectors, using insets of the phytoene desaturase (pds) gene. While in N. benthamiana the silencing phenotype was induced only by the construct carrying a 58-nt pds hairpin, in citrus plants all the constructs induced the silencing phenotype. Differences in the generation of secondary small interfering RNAs in both species are believed to be responsible for differential host-species effects. The ability of CLBV-based vectors to silence different endogenous citrus genes was further confirmed. Since CLBV-based vectors are known to be stable and induce VIGS in successive flushes for several months, these vectors provide an important genomic tool and it is expected that they will be useful to analyze gene function by reverse genetics in the long-lived citrus plants.
authors.affiliation: Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera Km. 4.5, Moncada, 46113 Valencia, Spain.
authors.forename: Jesus
authors.initials: J
authors.lastname: Agüero
authors.affiliation: Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera Km. 4.5, Moncada, 46113 Valencia, Spain.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Vives
authors.affiliation: Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera Km. 4.5, Moncada, 46113 Valencia, Spain.
authors.forename: Karelia
authors.initials: K
authors.lastname: Velázquez
authors.affiliation: Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera Km. 4.5, Moncada, 46113 Valencia, Spain.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Pina
authors.affiliation: Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera Km. 4.5, Moncada, 46113 Valencia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Navarro
authors.affiliation: Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera Km. 4.5, Moncada, 46113 Valencia, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Moreno
authors.affiliation: Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera Km. 4.5, Moncada, 46113 Valencia, Spain. Electronic address: jguerri@ivia.es.
authors.forename: Jose
authors.initials: J
authors.lastname: Guerri
mesh_terms: D002957:Citrus / Q000235:genetics / Q000378:metabolism / Q000821:virology; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics* / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D010942:Plant Viruses / Q000235:genetics / Q000502:physiology*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism / Q000821:virology
chemical_list: D010940:Plant Proteins
keywords: CLBV; Citrus; Nicotiana benthamiana; Silencing; VIGS; Viral vector