title: Association of MDR-TB isolates with clinical characteristics of patients from Northern region of India.
abstract: OBJECTIVE
  We sought to determine the characteristics and relative frequency of transmission of MDR-TB in North India and their association with the clinical and epidemiological characteristics of TB-patients.
  
  
  METHODS
  To achieve the objectives PCR-SSCP, MAS-PCR and direct DNA sequencing were used against 101 Mycobacterium tuberculosis isolates.
  
  
  RESULTS
  Multidrug-resistant-TB isolates were found to be significantly higher (P=0.000) in previously treated patients in comparison to newly diagnosed patients. Further, significant differences (P=0.003) were observed between different age groups (Mean±SD, 28.6±11.77) of the TB patients and multidrug resistance. Most frequent mutations were observed at codons 531 and 315 of rpoB and katG genes, respectively, in MDR-TB isolates.
  
  
  CONCLUSIONS
  Routine surveillance of resistance to anti-TB drugs will improve timely recognition of MDR-TB cases and help prevent further transmission in Northern India.
authors.forename: A
authors.initials: A
authors.lastname: Gupta
authors.forename: M R
authors.initials: MR
authors.lastname: Nagaraja
authors.forename: P
authors.initials: P
authors.lastname: Kumari
authors.forename: G
authors.initials: G
authors.lastname: Singh
authors.forename: R
authors.initials: R
authors.lastname: Raman
authors.forename: S K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Department of Microbiology, Institute of Medical Sciences, Uttar Pradesh, India.
authors.forename: S
authors.initials: S
authors.lastname: Anupurb
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics; D002374:Catalase / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D018562:Disease Transmission, Infectious; D016903:Drug Monitoring; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology / Q000635:transmission*; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit