title: Hepatoma-derived growth factor: from the bovine uterus to the in vitro embryo culture.
abstract: Early in cow embryo development, hepatoma-derived growth factor (HDGF) is detectable in uterine fluid. The origin of HDGF in maternal tissues is unknown, as is the effect of the induction on developing embryos. Herein, we analyze HDGF expression in day 8 endometrium exposed to embryos, as well as the effects of recombinant HDGF (rHDGF) on embryo growth. Exposure to embryos did not alter endometrial levels of HDGF mRNA or protein. HDGF protein localized to cell nuclei in the luminal epithelium and superficial glands and to the apical cytoplasm in deep glands. After uterine passage, levels of embryonic HDGF mRNA decreased and HDGF protein was detected only in the trophectoderm. In fetal fibroblast cultures, addition of rHDGF promoted cell proliferation. In experiments with group cultures of morulae in protein-free medium containing polyvinyl alcohol, adding rHDGF inhibited blastocyst development and did not affect cell counts when the morulae were early (day 5), whereas it enhanced blastocyst development and increased cell counts when the morulae were compact (day 6). In cultures of individual day 6 morulae, adding rHDGF promoted blastocyst development and increased cell counts. Our experiments with rHDGF indicate that the growth factor stimulates embryonic development and cell proliferation. HDGF is synthesized similarly by the endometrium and embryo, and it may exert embryotropic effects by autocrine and/or paracrine mechanisms.
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France egomez@serida.org.
authors.forename: E
authors.initials: E
authors.lastname: Gómez
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: E
authors.initials: E
authors.lastname: Correia-Álvarez
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: J N
authors.initials: JN
authors.lastname: Caamaño
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Díez
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: S
authors.initials: S
authors.lastname: Carrocera
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: N
authors.initials: N
authors.lastname: Peynot
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: D
authors.initials: D
authors.lastname: Martín
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Giraud-Delville
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: V
authors.initials: V
authors.lastname: Duranthon
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: O
authors.initials: O
authors.lastname: Sandra
authors.affiliation: Área de Genética y Reproducción AnimalCentro de Biotecnología Animal - SERIDA, Camino de Rioseco 1225, La Olla - Deva, 33394 Gijón, Asturias, Spain andINRAUMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France.
authors.forename: M
authors.initials: M
authors.lastname: Muñoz
mesh_terms: D000818:Animals; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C051610:hepatoma-derived growth factor