title: [Up-regulation of the CXCR4 expression by hypoxia in maxillary sinus carcinoma cells IMC3].
abstract: OBJECTIVE
  To investigate the expression of CXCR4 in maxillary sinus carcinoma cells IMC3 under hypoxia.
  
  
  METHODS
  IMC3 cells were cultured for 6 h, 12 h, 24 h and 48 h under normoxia and hypoxia. Real-Time PCR was applied to detect the expression of mRNA of CXCR4 and immunohistochemisrty was applied to investigate its protein level.
  
  
  RESULTS
  CXCR4 mRNA level was about 0.035 under normal conditions, which was obviously upregulated by hypoxia. The mRNA levels after culturing under hypoxia for 6 h, 12 h, 24 h and 48 h were 0.283, 0.313, 0.426, 0.510 respectively. There was statistically significant difference between the mRNA levels of each two groups (P < 0.05, Mann-Whiney Test) with a time dependent course, except for the difference between the groups of 6 h and 12 h. Immunohistochemistry showed that there was almost negative staining for CXCR4 in the cell cultured in nomoxia, while stong positive staining of CXCR4 was observed in cells cultured in hypoxia . The positive staining was located mainly in the cell membrane and cytoplasm and little in the nucleus.
  
  
  CONCLUSIONS
  Hypoxia could induce expression of CXCR4 in IMC3 cells at both mRNA and ptrotein levels. The upregulation of CXCR4 by hypoxia showed an obvious time dependent course.
authors.forename: Lizhong
authors.initials: L
authors.lastname: Su
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Zhang
authors.forename: Wenyue
authors.initials: W
authors.lastname: Ji
mesh_terms: D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008443:Maxillary Sinus / Q000378:metabolism* / Q000473:pathology; D019718:Receptors, CXCR4 / Q000378:metabolism*
chemical_list: C527276:CXCR4 protein, human; D019718:Receptors, CXCR4