title: Enhancement of broccoli indole glucosinolates by methyl jasmonate treatment and effects on prostate carcinogenesis.
abstract: Broccoli is rich in bioactive components, such as sulforaphane and indole-3-carbinol, which may impact cancer risk. The glucosinolate profile of broccoli can be manipulated through treatment with the plant stress hormone methyl jasmonate (MeJA). Our objective was to produce broccoli with enhanced levels of indole glucosinolates and determine its impact on prostate carcinogenesis. Brassica oleracea var. Green Magic was treated with a 250 μM MeJA solution 4 days prior to harvest. MeJA-treated broccoli had significantly increased levels of glucobrassicin, neoglucobrassicin, and gluconasturtiin (P < .05). Male transgenic adenocarcinoma of mouse prostate (TRAMP) mice (n = 99) were randomized into three diet groups at 5-7 weeks of age: AIN-93G control, 10% standard broccoli powder, or 10% MeJA broccoli powder. Diets were fed throughout the study until termination at 20 weeks of age. Hepatic CYP1A was induced with MeJA broccoli powder feeding, indicating biological activity of the indole glucosinolates. Following ∼ 15 weeks on diets, neither of the broccoli treatments significantly altered genitourinary tract weight, pathologic score, or metastasis incidence, indicating that broccoli powder at 10% of the diet was ineffective at reducing prostate carcinogenesis in the TRAMP model. Whereas broccoli powder feeding had no effect in this model of prostate cancer, our work demonstrates the feasibility of employing plant stress hormones exogenously to stimulate changes in phytochemical profiles, an approach that may be useful for optimizing bioactive component patterns in foods for chronic-disease-prevention studies.
authors.affiliation: 1 Division of Nutritional Sciences, Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign , Urbana, Illinois, USA .
authors.forename: Ann G
authors.initials: AG
authors.lastname: Liu
authors.forename: John A
authors.initials: JA
authors.lastname: Juvik
authors.forename: Elizabeth H
authors.initials: EH
authors.lastname: Jeffery
authors.forename: Lisa D
authors.initials: LD
authors.lastname: Berman-Booty
authors.forename: Steven K
authors.initials: SK
authors.lastname: Clinton
authors.forename: John W
authors.initials: JW
authors.lastname: Erdman
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D001937:Brassica / Q000187:drug effects / Q000378:metabolism*; D063646:Carcinogenesis / Q000187:drug effects*; D003517:Cyclopentanes / Q000494:pharmacology*; D005961:Glucosinolates / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D007211:Indoles / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008810:Mice, Inbred C57BL; D054883:Oxylipins / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010937:Plant Growth Regulators / Q000494:pharmacology; D011471:Prostatic Neoplasms / Q000188:drug therapy
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005961:Glucosinolates; D007211:Indoles; D054883:Oxylipins; D010936:Plant Extracts; D010937:Plant Growth Regulators; C555404:neoglucobrassicin; C443697:gluconasturtiin; C030374:indole; C072239:methyl jasmonate; C048308:glucobrassicin
keywords: Brassica oleracea; indole glucosinolates; indole-3-carbinol; neoglucobrassicin; sulforaphane