title: Attenuation and immunogenicity of a live high pathogenic PRRSV vaccine candidate with a 32-amino acid deletion in the nsp2 protein.
abstract: A porcine reproductive and respiratory syndrome virus (PRRSV) QY1 was serially passed on Marc-145 cells. Virulence of different intermediate derivatives of QY1 (P5, P60, P80, and P100) were determined. The study found that QY1 had been gradually attenuated during the in vitro process. Pathogenicity study showed that pigs inoculated with QY1 P100 and P80 did not develop any significant PRRS clinic symptoms. However, mild-to-moderate clinical signs and acute HP-PRRSV symptoms of infection were observed in pigs inoculated with QY1 P60 and P5, respectively. Furthermore, we determined the whole genome sequences of these four intermediate viruses. The results showed that after 100 passages, compared to QY1 P5, a total of 32 amino acid mutations were found. Moreover, there were one nucleotide deletion and a unique 34-amino acid deletion found at 5'UTR and in nsp2 gene during the attenuation process, respectively. Such deletions were genetically stable in vivo. Following PRRSV experimental challenge, pigs inoculated with a single dose of QY1 P100 developed no significant clinic symptoms and well tolerated lethal challenge, while QY1 P80 group still developed mild fever in the clinic trial after challenge. Thus, we concluded that QY1 P100 was a promising and highly attenuated PRRSV vaccine candidate.
authors.affiliation: College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Lu
authors.affiliation: College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Sun
authors.affiliation: College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, China.
authors.forename: Jianyue
authors.initials: J
authors.lastname: Mo
authors.affiliation: College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, China.
authors.forename: Xiduo
authors.identifier: 0000-0001-5964-8213
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Guangdong Wen's Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, Guangdong 527400, China.
authors.forename: Guanqun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Guangdong Wen's Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, Guangdong 527400, China.
authors.forename: Lianxiang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Guangdong Wen's Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, Guangdong 527400, China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, China.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Xie
authors.affiliation: College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, China ; Guangdong Wen's Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, Guangdong 527400, China.
authors.forename: Yinzuo
authors.initials: Y
authors.lastname: Bi
authors.affiliation: College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road, Guangzhou, Guangdong 510642, China.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Ma
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001706:Biopsy; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D009154:Mutation; D009500:Neutralization Tests; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000276:immunology*; D017384:Sequence Deletion; D013552:Swine; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D014766:Viremia
chemical_list: D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines