title: Assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant Aspergillus oryzae.
abstract: Anhydromevalonolactone (AMVL) is a bioactive natural product that arises from a molecular biology technique using Aspergillus oryzae as a heterologous host. AMVL has been used as a precursor for the synthesis of insect pest control reagents and has numerous applications in the biotechnological and medical industries. In this study, the Plackett-Burman Design and the Central Composite Design, which offer efficient and feasible approaches, were complemented to screen significant parameters and identify the optimal values for maximum AMVL production. The results suggested that sucrose, NaNO3, yeast extract and K2HPO4 were the key factors affecting AMVL production in a complex medium, whereas the major components required for a defined medium were NaNO3, K2HPO4, KH2PO4 and trace elements. These factors were subsequently optimized using the response surface methodology. Under optimal conditions, a maximum AMVL production of 250 mg/L in the complex medium and 200 mg/L in the defined medium was achieved, which represents an increase of approximately 3-4-fold compared to the commonly used malt extract medium.
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, 49 Soi Thianthale 25, Bangkhunthian-Chaithale Rd., Thakham, Bangkok 10150, Bangkhunthian, Thailand.
authors.forename: Songsak
authors.initials: S
authors.lastname: Wattanachaisaereekul
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, 49 Soi Thianthale 25, Bangkhunthian-Chaithale Rd., Thakham, Bangkok 10150, Bangkhunthian, Thailand.
authors.forename: Anuwat
authors.initials: A
authors.lastname: Tachaleat
authors.affiliation: Bioresource Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Klong Luang 12120, Pathum Thani, Thailand.
authors.forename: Juntira
authors.initials: J
authors.lastname: Punya
authors.affiliation: Bioresource Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Klong Luang 12120, Pathum Thani, Thailand.
authors.forename: Rachada
authors.initials: R
authors.lastname: Haritakun
authors.affiliation: Bioresource Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Klong Luang 12120, Pathum Thani, Thailand.
authors.forename: Chollaratt
authors.initials: C
authors.lastname: Boonlarppradab
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, 49 Soi Thianthale 25, Bangkhunthian-Chaithale Rd., Thakham, Bangkok 10150, Bangkhunthian, Thailand ; School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Soi Thianthale 25, Bangkhunthian-Chaithale Rd., Thakham, Bangkok 10150, Bangkhunthian, Thailand.
authors.forename: Supapon
authors.initials: S
authors.lastname: Cheevadhanarak
keywords: Anhydromevalonolactone; Aspergillus oryzae; Central composite design; Plackett-Burman design; Response surface methodology