title: Striatal dopamine dynamics in mice following acute and repeated toluene exposure.
abstract: BACKGROUND
  The abused inhalant toluene has potent behavioral effects, but only recently has progress been made in understanding the neurochemical actions that mediate the action of toluene in the brain. Available evidence suggests that toluene inhalation alters dopamine (DA) neurotransmission, but toluene's mechanism of action is unknown.
  
  
  OBJECTIVE
  The present study evaluated the effect of acute and repeated toluene inhalation (0, 2,000, or 4,000 ppm) on locomotor activity as well as striatal DA release and uptake using slice fast-scan cyclic voltammetry.
  
  
  RESULTS
  Acutely, 2,000 and 4,000 ppm toluene increased locomotor activity, while neurochemically only 4,000 ppm toluene potentiated electrically evoked DA release across the caudate-putamen and the nucleus accumbens. Repeated administration of toluene resulted in sensitization to toluene's locomotor activity effects. Brain slices obtained from mice repeatedly exposed to toluene demonstrated no difference in stimulated DA release in the caudate-putamen as compared to control animals. Repeated exposure to 2,000 and 4,000 ppm toluene caused a concentration-dependent decrease of 25-50 % in evoked DA release in the nucleus accumbens core and shell relative to air-exposed mice.
  
  
  CONCLUSIONS
  These voltammetric neurochemical findings following repeated toluene exposure suggest that there may be a compensatory downregulation of the DA system. Acute or repeated toluene exposure had no effect on the DA uptake kinetics. Taken together, these results demonstrate that acute toluene inhalation potentiates DA release, while repeated toluene exposure attenuates DA release in the nucleus accumbens only.
authors.affiliation: Department of Chemistry, Wayne State University, 5101 Cass Ave, Detroit, MI, 48202, USA.
authors.forename: Aaron K
authors.initials: AK
authors.lastname: Apawu
authors.forename: Tiffany A
authors.initials: TA
authors.lastname: Mathews
authors.forename: Scott E
authors.initials: SE
authors.lastname: Bowen
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects* / Q000502:physiology; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism; D009924:Organ Culture Techniques; D014050:Toluene / Q000008:administration & dosage* / Q000633:toxicity
chemical_list: D014050:Toluene; D004298:Dopamine