title: Transcriptomic and peptidomic analysis of protein hydrolysates from the white shrimp (L. vannamei).
abstract: An RNAseq approach associated to mass spectrometry was conducted to assess the composition, molecular mass distribution and primary sequence of hydrolytic peptides issued from hydrolysates of white shrimp (Litopenaeus vannamei) by-products. High performance size exclusion chromatography (HPSEC) analyses indicated that 69.2% of the 214-nm-absorbing components had apparent molecular masses below 1000 Da, and 88.3% below 2000 Da. OFFGEL-nLC-MALDI-TOF/TOF and nLC-ESI-MS/MS analyses led to the identification of 808 peptides based on the NCBI EST databank (161,397 entries) completed by the new L. vannamei databank (58,508 entries) that we created from the RNAs of tissues used for hydrolysate production. Whereas most of hydrolytic peptides have a MW below 2000 Da, preliminary investigations of antimicrobial properties revealed three antibacterial fractions that demonstrate functional activities. The abundance of small peptides as well as the biological activities detected could imply very interesting applications for shrimp hydrolysate in the field of aquaculture feeding.
authors.affiliation: Université de Caen Basse-Normandie, UMR BOREA, IBFA, F-14032 Caen, France; UMR BOREA Biologie des ORganismes et Ecosystèmes Aquatiques" MNHN, UPMC, UCBN, CNRS-7208, IRD-207, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Robert
authors.affiliation: Université de Caen Basse-Normandie, UMR BOREA, IBFA, F-14032 Caen, France; UMR BOREA Biologie des ORganismes et Ecosystèmes Aquatiques" MNHN, UPMC, UCBN, CNRS-7208, IRD-207, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Zatylny-Gaudin
authors.affiliation: Aquativ (Diana Group), ZA du Gohelis, 56250 Elven, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Fournier
authors.affiliation: ABiMS, FR2424 CNRS-UPMC, Station Biologique, 29680 Roscoff, France.
authors.forename: Erwan
authors.initials: E
authors.lastname: Corre
authors.affiliation: ABiMS, FR2424 CNRS-UPMC, Station Biologique, 29680 Roscoff, France.
authors.forename: Gildas
authors.initials: G
authors.lastname: Le Corguillé
authors.affiliation: Proteogen, SF 4206 ICORE, Université de Caen Basse-Normandie, F-14032 Caen, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Bernay
authors.affiliation: Université de Caen Basse-Normandie, UMR BOREA, IBFA, F-14032 Caen, France; UMR BOREA Biologie des ORganismes et Ecosystèmes Aquatiques" MNHN, UPMC, UCBN, CNRS-7208, IRD-207, France; Proteogen, SF 4206 ICORE, Université de Caen Basse-Normandie, F-14032 Caen, France. Electronic address: joel.henry@unicaen.fr.
authors.forename: Joël
authors.initials: J
authors.lastname: Henry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002853:Chromatography, Liquid; D020869:Gene Expression Profiling; D006868:Hydrolysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000737:chemistry*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D020543:Proteome; D040901:Proteomics; D020539:Sequence Analysis, Protein
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D011492:Protein Hydrolysates; D020543:Proteome
keywords: Antimicrobial activity; Litopenaeus vannamei hydrolysate; Mass spectrometry; OFFGEL electrophoresis; RNAseq