title: Toxic effects of paraquat on cytokine expression in common carp, Cyprinus carpio L.
abstract: In this study, the acute toxicity of paraquat (PQ) in common carp was determined. Then, the contents and mRNA levels of the cytokines interleukin-1β (IL-1β), interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) were evaluated following subacute exposure to PQ. The results show that the LC50 of PQ for common carp at 72 and 96 h was 15.962 and 15.106 mg/L, respectively. Moreover, after 7 days of subacute PQ exposure, the IL-1β content in the fish liver and kidney increased, although it decreased in spleen. However, changes in the IFN-γ content showed an irregular trend. The TNF-α content increased in the liver and spleen but decreased in the kidney. Additionally, PQ exposure also induced alterations in the mRNA levels of IL-1β, IFN-γ, and TNF-α. These results suggest that PQ exposure may result in suppression or excessive activation of the immune system in treated fish and lead to immune dysfunction and reduced immunity.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, People's Republic of China.
authors.forename: Junguo
authors.initials: J
authors.lastname: Ma
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.forename: Daichun
authors.initials: D
authors.lastname: Niu
mesh_terms: D000818:Animals; D002347:Carps / Q000276:immunology* / Q000378:metabolism; D002681:China; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010269:Paraquat / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics; D013154:Spleen / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D010269:Paraquat
keywords: Acute Toxicity; Common Carp; Cytokine; Immunotoxicity; Paraquat