title: Using Novel Method to Detect Different Cancer-Cell Stages of Model Human Lung Carcinoma.
abstract: BACKGROUND
  Synchrotron radiation infrared (SR-IR) microspectroscopy and SR-IR spectroscopic imaging are extremely valuable techniques for determining the molecular composition of biological and biomedical samples. In this work, SR-IR is applied in the study of the lung cancer cells in different cell cycles.
  
  
  METHODS
  We use a novel synchrotron based radiation infrared system combined synchronized model human lung carcinoma to reveal its unique character pattern.
  
  
  RESULTS
  After using SR-IR microspectroscopy, we discovered that the ratio of protein to lipid in G1 and G2 states is around 4.0 and 6.1, respectively. Moreover, for the DNA at the wavenumber position of 1225 cm(-1) , the intensity ratio of G2 state to G1 state is approximately 1.6. These data indicate that the cell in G1 state has more lipid composition to prepare for the DNA synthesis, but the cell in G2 state has more protein composition to prepare for the mitosis. The cell has larger DNA concentration in G2 state, which can be explained for the DNA synthesis.
  
  
  CONCLUSIONS
  Through our research, we demonstrate that different growth state of cancer cell presenting unique functional groups concentration profiles and distribution via using SR-IR microspectrometry. These applications will provide another ways to improve modern cancer screening in the future.
authors.affiliation: Department of Materials Science and Engineering, National Chiao Tung University, Taiwan, ROC.
authors.forename: Hao-Yuan
authors.initials: HY
authors.lastname: Cheng
authors.affiliation: Department of Materials Science and Engineering, National Chiao Tung University, Taiwan, ROC.
authors.forename: Fu-Hsiang
authors.initials: FH
authors.lastname: Ko
authors.affiliation: National Synchrotron Radiation Research Center, Taiwan, ROC.
authors.forename: Lee-Jene
authors.initials: LJ
authors.lastname: Lai
mesh_terms: D000577:Amides / Q000032:analysis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008055:Lipids / Q000032:analysis; D008175:Lung Neoplasms / Q000473:pathology*; D008954:Models, Biological; D009367:Neoplasm Staging; D013055:Spectrophotometry, Infrared / Q000379:methods*; D017356:Synchrotrons
chemical_list: D000577:Amides; D008055:Lipids
keywords: cell cycle; lung carcinoma; microspectrometry; synchrotron; synchrotron radiation infrared