title: MiR-204 promotes apoptosis in oxidative stress-induced rat Schwann cells by suppressing neuritin expression.
abstract: Neuritin (Nrn1) is a neurotrophin that plays an important role in nervous system plasticity and repair following nerve injury. MicroRNAs (miRNAs) are a type of small non-coding RNA that regulate nearly all aspects of nerve development and survival, including apoptosis. Here it was found that miR-204 negatively regulates Nrn1 protein expression through direct interaction with Nrn1 transcript. Moreover, miR-204 activates cleaved caspase-3, enhancing the sensitivity of RSC96 Schwann cells to H2O2-induced oxidative stress and apoptosis. Thus, miR-204 expressed at a low level may create a microenvironment suitable for the repair of injured nerves by relieving the inhibition of Nrn1 transcription and stimulating the anti-apoptotic function of Schwann cells. These results provide novel insights into the roles of miR-204 in nerve injury and repair.
authors.affiliation: Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Department of Basic Medical Science, School of Medicine, Shihezi University, Shihezi 832000, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
authors.affiliation: Key Laboratories of Sheep Breeding and Reproduce, Xinjiang Academy of Agricultural And Reclamation Science, Shihezi 832000, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Basic Medical Science, School of Medicine, Shihezi University, Shihezi 832000, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Nie
authors.affiliation: Department of Basic Medical Science, School of Medicine, Shihezi University, Shihezi 832000, China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Jian
authors.affiliation: Key Laboratories of Sheep Breeding and Reproduce, Xinjiang Academy of Agricultural And Reclamation Science, Shihezi 832000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Basic Medical Science, School of Medicine, Shihezi University, Shihezi 832000, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Liao
authors.affiliation: Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Haiyuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Department of Basic Medical Science, School of Medicine, Shihezi University, Shihezi 832000, China. Electronic address: huangjin623@163.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Laboratories of Sheep Breeding and Reproduce, Xinjiang Academy of Agricultural And Reclamation Science, Shihezi 832000, China. Electronic address: shangquangan@163.com.
authors.forename: Shangquan
authors.initials: S
authors.lastname: Gan
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009479:Neuropeptides / Q000235:genetics*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D058851:GPI-Linked Proteins; C549404:MIRN204 microRNA, human; D035683:MicroRNAs; D009479:Neuropeptides; C105161:Nrn1 protein, rat; D006861:Hydrogen Peroxide
keywords: Apoptosis; MicroRNA; Nerve injury and repair; Neuritin