title: Quantitative characterization of the impact of pulp refining on enzymatic saccharification of the alkaline pretreated corn stover.
abstract: In this work, corn stover was refined by a pulp refining instrument (PFI refiner) after NaOH pretreatment under varied conditions. The quantitative characterization of the influence of PFI refining on enzymatic hydrolysis was studied, and it was proved that the enhancement of enzymatic saccharification by PFI refining of the pretreated corn stover was largely due to the significant increment of porosity of substrates and the reduction of cellulose crystallinity. Furthermore, a linear relationship between beating degree and final total sugar yields was found, and a simple way to predict the final total sugar yields by easily testing the beating degree of PFI refined corn stover was established. Therefore, this paper provided the possibility and feasibility for easily monitoring the fermentable sugar production by the simple test of beating degree, and this will be of significant importance for the monitoring and controlling of industrial production in the future.
authors.affiliation: CAS Key Laboratory of Bio-Based Material, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Huanfei
authors.initials: H
authors.lastname: Xu
authors.affiliation: CAS Key Laboratory of Bio-Based Material, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Bio-Based Material, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, People's Republic of China.
authors.forename: Xindong
authors.initials: X
authors.lastname: Mu
authors.affiliation: CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yu
authors.affiliation: CAS Key Laboratory of Bio-Based Material, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, People's Republic of China.
authors.forename: Yuedong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, People's Republic of China. Electronic address: wanghs@qibebt.ac.cn.
authors.forename: Haisong
authors.initials: H
authors.lastname: Wang
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002138:Calibration; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000378:metabolism*; D003460:Crystallization; D005936:Glucans / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D010209:Paper; D016062:Porosity; D012972:Sodium Hydroxide / Q000494:pharmacology*; D014866:Waste Products; D014990:Xylans / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000187:drug effects; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D005936:Glucans; D014866:Waste Products; D014990:Xylans; D012972:Sodium Hydroxide; D001617:beta-Glucosidase; D002480:Cellulase
keywords: Alkaline pretreatment; Corn stover; Enzymatic hydrolysis; PFI refining; Quantitative characterization