title: The simultaneous detection of free and total prostate antigen in serum samples with high sensitivity and specificity by using the dual-channel surface plasmon resonance.
abstract: Free/total prostate antigen (f/t-PSA) ratio in serum as a promising parameter has been used to improve the differentiation of benign and malignant prostate disease. In order to obtain the accurate and reliable f/t-PSA ratio, the simultaneous detection of f-PSA and t-PSA with high sensitivity and specificity is required. In this work, the dual-channel surface plasmon resonance (SPR) has been employed to meet the requirement. In one channel, t-PSA was directly measured with a linear range from 1.0 to 20.0 ng/mL. In another channel, due to the low concentration of f-PSA in serum, the asynchronous competitive inhibition immunoassay with f-PSA@Au nanoparticles (AuNPs) was developed. As expected, the detection sensitivity of f-PSA was greatly enhanced, and a linear correlation with wider linear range from 0.010 to 0.40 ng/mL was also achieved. On the other hand, a simple method was explored for significantly reducing the non-specific adsorption of co-existing proteins. On basis of this, the f/t-PSA ratios in serum samples from prostate cancer (PCa) or benign prostatic hyperplasia (BPH) patients were measured. And it was found that there was significant difference between the distributions of f/t-PSA ratio in BPH patients (16.44±1.77%) and those in PCa patients (24.53±4.97%). This present work provides an effective method for distinguishing PCa from BPH, which lays a potential foundation for the early diagnosis of PCa.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Zhongxiu
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Qin
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Peng
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Shu
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: dengchunyan@csu.edu.cn.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Deng
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: xiangj@csu.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Xiang
mesh_terms: D001667:Binding, Competitive; D003937:Diagnosis, Differential; D042241:Early Diagnosis; D006046:Gold; D006801:Humans; D007610:Kallikreins / Q000097:blood*; D008297:Male; D053768:Metal Nanoparticles; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000097:blood*; D011470:Prostatic Hyperplasia / Q000097:blood / Q000175:diagnosis; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data
chemical_list: D006046:Gold; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen
keywords: Free/total prostate antigen ratio; Prostate cancer; Signal amplification; Simultaneous detection; Surface plasmon resonance