title: Detection of quorum sensing activity in the multidrug-resistant clinical isolate Pseudomonas aeruginosa strain GB11.
abstract: A multidrug-resistant clinical bacteria strain GB11 was isolated from a wound swab on the leg of a patient. Identity of stain GB11 as Pseudomonas aeruginosa was validated by using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Detection of the production of signaling molecules, N-acylhomoserine lactones (AHLs), was conducted using three different bacterial biosensors. A total of four different AHLs were found to be produced by strain GB11, namely N-butyryl homoserine lactone (C4-HSL), N-hexanoylhomoserine lactone (C6-HSL), N-octanoyl homoserine lactone (C8-HSL) and N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL) using high resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these detected AHLs, 3-oxo-C12-HSL was found to be the most abundant AHL produced by P. aeruginosa GB11.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. chenghj90@gmail.com.
authors.forename: Huey Jia
authors.initials: HJ
authors.lastname: Cheng
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. robsonee@live.com.
authors.forename: Robson
authors.initials: R
authors.lastname: Ee
authors.affiliation: Jeffrey Cheah School of Medicine & Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor Darul Ehsan, Malaysia. cheong.yuet.meng@monash.edu.
authors.forename: Yuet Meng
authors.initials: YM
authors.lastname: Cheong
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. tmarilyn36@gmail.com.
authors.forename: Wen-Si
authors.initials: WS
authors.lastname: Tan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. yinwaifong@yahoo.com.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. kokgan@um.edu.my.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D053038:Quorum Sensing / Q000502:physiology*; D053719:Tandem Mass Spectrometry / Q000379:methods