title: Matrix metalloproteinase-8 promoter gene polymorphisms in Mexican women with ovarian cancer.
abstract: Increased levels of matrix metalloproteinase-8 (MMP-8) have been associated with tumor grade and stage in ovarian cancer. Also, it has been reported that higher concentrations of this enzyme in fluid from malignant ovarian cysts compared with benign ovarian cysts. However, no genetic analysis has been conducted yet to assess the contribution of MMP-8 polymorphisms in ovarian cancer. Thus, this study was performed to investigate the frequencies of MMP-8 genotypes in Mexican women with ovarian cancer. MMP-8 promoter genotypes were examined in 35 malignant ovarian tumors, 51 benign tumors, and 37 normal ovary tissues. Two single nucleotide polymorphisms were selected and characterized using polymerase chain reaction-restriction fragment length polymorphism analysis. The chi-square test was used to calculate statistical significance. Haplotype analysis was performed using the SNPstats web tool. Of the two polymorphisms, only the MMP-8 -799 T/T genotype was significantly associated with an increased risk of ovarian cancer (OR 3.78, 95 % CI 1.18-12.13). The Kaplan-Meier analysis for this polymorphism showed that patients with the T/T genetic variant had a tendency toward significant worse overall survival compared with patients with the C/C + C/T genotypes. Haplotype analysis revealed no significant differences in haplotype distribution between benign ovarian tumors, malignant ovarian cancer, and controls. This study suggests that MMP-8 promoter gene polymorphism -799 T/T is significantly associated with an increased risk of ovarian cancer in Mexican women.
authors.affiliation: Research Unit in Reproductive Medicine, UMAE Hospital de Gineco-Obstetricia No. 4 Luis Castelazo Ayala, IMSS, Rio Magdalena 289, Sexto Piso, Tizapan San Angel, CP 01090, México, D. F., Mexico.
authors.forename: Fabian
authors.initials: F
authors.lastname: Arechavaleta-Velasco
authors.forename: Rocio
authors.initials: R
authors.lastname: Cuevas-Antonio
authors.forename: Pablo
authors.initials: P
authors.lastname: Dominguez-Lopez
authors.forename: Isaias
authors.initials: I
authors.lastname: Estrada-Moscoso
authors.forename: Fazlollah Shahram
authors.initials: FS
authors.lastname: Imani-Razavi
authors.forename: Moises
authors.initials: M
authors.lastname: Zeferino-Toquero
authors.forename: Laura
authors.initials: L
authors.lastname: Diaz-Cueto
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D053208:Kaplan-Meier Estimate; D020784:Matrix Metalloproteinase 8 / Q000235:genetics*; D008800:Mexico; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000401:mortality; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic
chemical_list: C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8