title: Efficient screening of environmental isolates for Saccharomyces cerevisiae strains that are suitable for brewing.
abstract: We developed an efficient screening method for Saccharomyces cerevisiae strains from environmental isolates. MultiPlex PCR was performed targeting four brewing S. cerevisiae genes (SSU1, AWA1, BIO6, and FLO1). At least three genes among the four were amplified from all S. cerevisiae strains. The use of this method allowed us to successfully obtain S. cerevisiae strains.
authors.affiliation: a Faculty of Food and Nutrition, Department of Food and Fermentation Science , Beppu University , Oita , Japan.
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Fujihara
authors.forename: Mika
authors.initials: M
authors.lastname: Hino
authors.forename: Hideharu
authors.initials: H
authors.lastname: Takashita
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kajiwara
authors.forename: Keiko
authors.initials: K
authors.lastname: Okamoto
authors.forename: Kensuke
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D001515:Beer / Q000382:microbiology*; D004777:Environment; D005285:Fermentation; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
keywords: MultiPlex PCR; S. cerevisiae; brewing yeast; screening