title: Chemical composition and anti-inflammatory activity of Chamaecyparis obtusa f.formosana wood essential oil from Taiwan.
abstract: Chamaecyparis obtusa Siebold & Zucc. f.formosana Hayata (Cupressaceae) is one of the treasured conifers in Taiwan. In this study, the wood essential oil of the species was extracted and its anti-inflammatory bioactivities analyzed. The composition analysis of the essential oil yielded a total of 57 compounds with alpha-terpineol (19.4%), tau-muurolol (16.9%), borneol (16%), and alpha-cadinol (10.9%) predominating. The anti-inflammatory assays of the essential oil suggest that C obtusa f. formnosana wood oil has no cytotoxicity, and was capable of inhibiting the expressions of tumor necrosis factor (TNF-alpha), IL-6, IL-1, pro IL-1beta, NO, iNOS, and NLRP3 in murine macrophage cells. It was unable, however, to inhibit the expression of COX-2. Further investigation of the effect of the essential oil on the cellular signal transduction indicated that it was capable of inhibiting ERKI/2, JNKI/2 and p38; it also showed excellent inhibition against ROS. Thus, the overall results indicated that C. obtusa f.formnosana wood oil had very good anti-inflammatory efficacies.
authors.forename: Tzu-Chao
authors.initials: TC
authors.lastname: Chien
authors.forename: Sheng-Fong
authors.initials: SF
authors.lastname: Lo
authors.forename: Chen-Lung
authors.initials: CL
authors.lastname: Ho
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D029780:Chamaecyparis / Q000737:chemistry*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D009822:Oils, Volatile / Q000032:analysis / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013624:Taiwan
chemical_list: D000893:Anti-Inflammatory Agents; D052246:Cyclooxygenase 2 Inhibitors; D009822:Oils, Volatile; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D020928:Mitogen-Activated Protein Kinases