title: Characterization and expression pattern of a novel β-defensin in common carp (Cyprinus carpio L.): implications for its role in mucosal immunity.
abstract: β-defensins are a group of cysteine-rich cationic antimicrobial peptides that play antibacterial and antiviral roles in immune systems of vertebrates. Here, we report the cloning and identification of a β-defensin 3 cDNA sequence from the common carp (Cyprinus carpio L.). Sequence alignment and phylogenetic analysis indicated that this β-defensin 3 belonged to the BD-2 group of fish. Real-time PCR showed that the β-defensin 3 mRNA was expressed in all the tissues of normal common carp that we examined and was highly expressed in the spleen and gills. When challenged with Vibrio anguillarum, the expression level of common carp β-defensin 3 mRNA was quickly upregulated in various tissues. Our results indicate that the β-defensin 3 showed markedly high constitutive expression in the gills, and significantly upregulated expression in the hindgut of the common carp after infection, suggesting it plays an important role in the innate and mucosal immunity of common carp.
authors.affiliation: a Shandong Provincial Key Laboratory of Animal Resistance Biology , College of Life Science, Shandong Normal University , Jinan , China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Hongyan
authors.initials: H
authors.lastname: Guo
authors.forename: Shijuan
authors.initials: S
authors.lastname: Shan
authors.forename: Chenchen
authors.initials: C
authors.lastname: Qi
authors.forename: Liguo
authors.initials: L
authors.lastname: An
authors.forename: Guiwen
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D018928:Immunity, Mucosal / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis; D016415:Sequence Alignment; D023083:beta-Defensins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D023083:beta-Defensins
keywords: common carp (Cyprinus carpio L.); innate immunity; mRNA expression; molecular cloning; β-defensin