title: Far upstream element-binding protein 1(FUBP1) expression differs between human colorectal cancer and non-cancerous tissue.
abstract: Presented study aimed to detect the expression of far upstream element-binding protein 1 (FUBP1) in clinical samples of colorectal carcinoma (CRC) and explore the correlations of their expression with the clinicopathological characteristics of CRC.The streptavidin-perosidase (SP) method of immunohistochemistry was used to detect the expression of FUBP1 in 34 cases of colorectal cancer and their surrounding surrounding normal tissue, 30 cases of adenoma tissue. Using fluorescent quantitative reverse transcription polymerase chain reaction (qPCR), the expression of FUBP1 mRNA was measured in colorectal cancer and its surrounding normal tissue from 32 patients. FUBP1 protein expression level was detected by the Western blot method in 32 pairs of colorectal cancer tissue and surrounding normal tissue, and 30 cases of adenoma tissue.The positive rate of FUBP1 was detected through histochemistry in colorectal carcinomas (82.3%) which was higher than that in colorectal adenomas (46.7%) and surrounding normal tissues (20.5%). The relative amount of FUBP1 mRNA by qPCR method in colorectal carcinoma tissues (0.2703±0.1118) was higher than that of surrounding normal tissues (0.1898±0.0635; P<0.05). The Western blot showed that FUBP1 was mainly expressed in colorectal carcinoma tissues (0.6499±0.1473),which barely expressed in adenoma tissues (0.3756±0.1377; P<0.05) and surrounding normal tissues (0.1675±0.0613; P < 0.05).FUBP1 expression differs among colorectal tissues, which is overexpressed in colorectal carcinoma tissue. Further studies are needed to explore the role of FUBP1 in the pathogenesis of colorectal carcinoma.
authors.forename: M Y
authors.initials: MY
authors.lastname: Jia
authors.forename: Y J
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology; D004265:DNA Helicases / Q000032:analysis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004268:DNA-Binding Proteins; C492258:FUBP1 protein, human; D016601:RNA-Binding Proteins; D004265:DNA Helicases