title: Detection of S-adenosylhomocysteine and methylation index in blood by capillary electrophoresis.
abstract: This work proposes an approach to the direct analysis of S-adenosylhomocysteine (SAH) and the methylation index in blood using CE with UV detection (CE-UV). After application of meglumine postinjection, we achieved SAH in-capillary preconcentration in the HClO4 extracts of erythrocytes, which improved the detection limit (S/N = 3) of SAH up to 3 fmol or 180 nmol/L at the injection volume of 50 nL, taking into account the sample dilution rate. CE-UV was carried out in 30 mM glycine and 45 mmol/L HCl (pH ~1.8) at 17 kV in a capillary 48 cm in length and 50 μm id. Accuracy of the technique was 101% and reproducibility was about 12%.
authors.affiliation: FSBI "Institute of general pathology and pathophysiology RAMS", Moscow, Russia.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Ivanov
authors.forename: Boris P
authors.initials: BP
authors.lastname: Luzyanin
authors.forename: Edward D
authors.initials: ED
authors.lastname: Virus
authors.forename: Anna S
authors.initials: AS
authors.lastname: Rotkina
authors.forename: Aslan A
authors.initials: AA
authors.lastname: Kubatiev
mesh_terms: D000293:Adolescent; D000328:Adult; D019075:Electrophoresis, Capillary / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008536:Meglumine; D008745:Methylation; D010472:Perchlorates / Q000737:chemistry; D015203:Reproducibility of Results; D012435:S-Adenosylhomocysteine / Q000097:blood* / Q000737:chemistry; D055815:Young Adult
chemical_list: D010472:Perchlorates; D008536:Meglumine; D012435:S-Adenosylhomocysteine; C576518:Perchloric Acid
keywords: Blood; CE; Meglumine; Methylation index; S-adenosylhomocysteine; S-adenosylmethionine