title: Frozen adipose-derived mesenchymal stem cells maintain high capability to grow and differentiate.
abstract: In recent years, there has been a shift toward tissue-engineering strategies using stem cells for plastic and reconstructive surgical procedures. Therefore, it is important to develop safe and reproducible protocols for the extraction of adipose-derived stromal cells (ASCs) to allow cells to be stored in liquid nitrogen for future needs. The aspirated liposuction obtained from healthy donors were immediately processed after the suction using a protocol developed in our laboratory. The resulting stromal vascular fraction (SVF) was then characterized by the presence of adipose-derived stromal cells, at later stage frozen in liquid nitrogen. After that, cells were thawed and again characterized by adipose-derived stromal cells, cellular survival, differentiation ability and Colony Forming Unit-Fibroblast like colonies (CFU-F). Extraction and freezing of cells contained in the stromal vascular fraction demonstrate that thawed cells maintain the full capability to grow and differentiate in culture. The advent of adipose-derived stromal cells use in tissue engineering will assume a wide role in esthetic restoration in plastic surgery. It is thus important to develop clinically translatable protocols for the preparation and storage of adipose-derived stromal cells. Our results show that adipose-derived stromal cells in serum free can easily be frozen and stored in liquid nitrogen with retention of 85% of cell viability and 180,890 cell/g yield plus normal proliferative capacity and differentiation potential compared with fresh controls. These observations set the basis for adipose-derived stromal cells banking.
authors.affiliation: Swiss Stem Cell Foundation, In Pasquée, 6925 Gentilino, Switzerland.
authors.forename: Greta
authors.initials: G
authors.lastname: Minonzio
authors.affiliation: Swiss Stem Cell Foundation, In Pasquée, 6925 Gentilino, Switzerland.
authors.forename: Mattia
authors.initials: M
authors.lastname: Corazza
authors.affiliation: Swiss Stem Cell Foundation, In Pasquée, 6925 Gentilino, Switzerland.
authors.forename: Luca
authors.initials: L
authors.lastname: Mariotta
authors.affiliation: Molecular Diagnostic Laboratory, In Pasquée, 6925 Gentilino, Switzerland.
authors.forename: Mauro
authors.initials: M
authors.lastname: Gola
authors.affiliation: Centre de Chirurgie Plastique, Lausanne, Switzerland.
authors.forename: Michele
authors.initials: M
authors.lastname: Zanzi
authors.affiliation: Plastic Surgery, Academia Day Clinic, Chiasso, Switzerland.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Gandolfi
authors.affiliation: Via Visconti di Modrone 8/10, Milano, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: De Fazio
authors.affiliation: Swiss Stem Cell Foundation, In Pasquée, 6925 Gentilino, Switzerland. Electronic address: gianni.soldati@gmail.com.
authors.forename: Gianni
authors.initials: G
authors.lastname: Soldati
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000293:Adolescent; D000328:Adult; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005615:Freezing; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D055815:Young Adult
keywords: Adipose stem cells; Adipose-derived stem cells; Cryopreservation; Human cell therapy; Mesenchymal stem cells; Tissue banking; Tissue engineering