title: Optimization of polysaccharides extraction from Clematis huchouensis Tamura and its antioxidant activity.
abstract: In this study, response surface methodology (RSM), based on Box-Behnken design, was employed to optimize the extraction conditions of polysaccharide from Clematis huchouensis Tamura (CP). And then the antioxidant activities of the samples were investigated including scavenging effects of superoxide and hydroxyl radicals and their reducing power. The results of chemical composition and FT-IR spectrum analysis showed the polysaccharide was acidic proteoglycan. And moreover, CP showed excellent antioxidant activity in these three assays. The purification and structure of CP need to be further studied.
authors.affiliation: Department of Medicine, Huzhou Teachers College, Huzhou 313000, China. Electronic address: zhzs@hutc.zj.cn.
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Medicine, Huzhou Teachers College, Huzhou 313000, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Medicine, Huzhou Teachers College, Huzhou 313000, China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Medicine, Huzhou Teachers College, Huzhou 313000, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D039324:Clematis / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical
keywords: Antioxidant activities; Clematis huchouensis Tamura; Polysaccharide; Response surface methodology