title: Determination of bisphenol A, 4-octylphenol, and 4-nonylphenol in soft drinks and dairy products by ultrasound-assisted dispersive liquid-liquid microextraction combined with derivatization and high-performance liquid chromatography with fluorescence detection.
abstract: A novel hyphenated method based on ultrasound-assisted dispersive liquid-liquid microextraction coupled to precolumn derivatization has been established for the simultaneous determination of bisphenol A, 4-octylphenol, and 4-nonylphenol by high-performance liquid chromatography with fluorescence detection. Different parameters that influence microextraction and derivatization have been optimized. The quantitative linear range of analytes is 5.0-400.0 ng/L, and the correlation coefficients are more than 0.9998. Limits of detection for soft drinks and dairy products have been obtained in the range of 0.5-1.2 ng/kg and 0.01-0.04 μg/kg, respectively. Relative standard deviations of intra- and inter-day precision for retention time and peak area are in the range of 0.47-2.31 and 2.76-8.79%, respectively. Accuracy is satisfactory in the range of 81.5-118.7%. Relative standard deviations of repeatability are in the range of 0.35-1.43 and 2.36-4.75% for retention time and peak area, respectively. Enrichment factors for bisphenol A, 4-octylphenol, and 4-nonylphenol are 170.5, 240.3, and 283.2, respectively. The results of recovery and matrix effect are in the range of 82.7-114.9 and 92.0-109.0%, respectively. The proposed method has been applied to the determination of bisphenol A, 4-octylphenol, and 4-nonylphenol in soft drinks and dairy products with much higher sensitivity than many other methods.
authors.affiliation: Shandong Provincial Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, P.R. China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lv
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhao
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhu
authors.forename: Fei
authors.initials: F
authors.lastname: Qu
authors.forename: Cuihua
authors.initials: C
authors.lastname: Song
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
authors.forename: Yourui
authors.initials: Y
authors.lastname: Suo
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis*; D002253:Carbonated Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003611:Dairy Products / Q000032:analysis*; D005453:Fluorescence; D059627:Liquid Phase Microextraction; D033401:Microscopy, Acoustic; D010636:Phenols / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; C080417:4-octylphenol; C041594:4-nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Derivatization; Endocrine disruptors; Fluorescence detection; Food analysis; Microextraction