title: Local Renin-Angiotensin system in normal hematopoietic and multiple myeloma-related progenitor cells.
abstract: OBJECTIVE
  The prominent functions of the local renin-angiotensin system (RAS) in primitive hematopoiesis further support the hypothesis that local autocrine bone marrow RAS could also be active in neoplastic hematopoiesis. The aim of this study is to examine critical RAS elements in normal CD34+ hematopoietic stem cells and multiple myeloma (MM)-related progenitor cells.
  
  
  METHODS
  The study group comprised the total bone marrow cells (CBM) of 10 hematologically normal people, the CD34+ stem cell samples (CD34+CBM) of 9 healthy donors for allogeneic peripheral stem cell transplantation, and the CD34+ stem cell samples (CD34+MM) of 9 MM patients undergoing autologous peripheral stem cell transplantation. We searched for the gene expression of the major RAS components in healthy hematopoietic cells and myeloma cells by quantitative real-time polymerase chain reaction analysis.
  
  
  RESULTS
  RENIN, angiotensinogen (ANGTS), and angiotensin converting enzyme-I (ACE I) mRNA expression levels of CBM were significantly higher than those in myeloma patients (p=0.03, p=0.002, and p=0.0008, respectively). Moreover, RENIN and ANGTS mRNA expression levels were significantly higher in CD34+ stem cell samples of healthy allogeneic donors compared to those in myeloma patients (p=0.001 and p=0.01). However, ACE I expression levels were similar in CD34+CBM and CD34+MM hematopoietic cells (p=0.89).
  
  
  CONCLUSIONS
  Although found to be lower than in the CBM and CD34+CBM hematopoietic cells, the local RAS components were also expressed in CD34+MM hematopoietic cells. This point should be kept in mind while focusing on the immunobiology of MM and the processing of autologous cells during the formation of transplantation treatment protocols.
authors.affiliation: Hacettepe University Faculty of Medicine, Department of Internal Medicine, Division of Hematology, Ankara, Turkey.
authors.forename: Burak
authors.initials: B
authors.lastname: Uz
authors.affiliation: İstanbul University, Institute for Experimental Medicine Research, Department of Genetics, İstanbul, Turkey.
authors.forename: Suzin Çatal
authors.initials: SÇ
authors.lastname: Tatonyan
authors.affiliation: İstanbul University, Institute for Experimental Medicine Research, Department of Genetics, İstanbul, Turkey.
authors.forename: Müge
authors.initials: M
authors.lastname: Sayitoğlu
authors.affiliation: İstanbul University, Institute for Experimental Medicine Research, Department of Genetics, İstanbul, Turkey.
authors.forename: Yücel
authors.initials: Y
authors.lastname: Erbilgin
authors.affiliation: İstanbul University, Institute for Experimental Medicine Research, Department of Genetics, İstanbul, Turkey.
authors.forename: Ozden
authors.initials: O
authors.lastname: Hatırnaz
authors.affiliation: Hacettepe University Faculty of Medicine, Department of Internal Medicine, Division of Hematology, Ankara, Turkey.
authors.forename: Salih
authors.initials: S
authors.lastname: Aksu
authors.affiliation: Hacettepe University Faculty of Medicine, Department of Internal Medicine, Division of Hematology, Ankara, Turkey.
authors.forename: Yahya
authors.initials: Y
authors.lastname: Büyükaşık
authors.affiliation: Hacettepe University Faculty of Medicine, Department of Internal Medicine, Division of Hematology, Ankara, Turkey.
authors.forename: Nilgün
authors.initials: N
authors.lastname: Sayınalp
authors.affiliation: Hacettepe University Faculty of Medicine, Department of Internal Medicine, Division of Hematology, Ankara, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Göker
authors.affiliation: Hacettepe University Faculty of Medicine, Department of Internal Medicine, Division of Hematology, Ankara, Turkey.
authors.forename: Osman İ
authors.initials: Oİ
authors.lastname: Ozcebe
authors.affiliation: İstanbul University, Institute for Experimental Medicine Research, Department of Genetics, İstanbul, Turkey.
authors.forename: Uğur
authors.initials: U
authors.lastname: Ozbek
authors.affiliation: Hacettepe University Faculty of Medicine, Department of Internal Medicine, Division of Hematology, Ankara, Turkey.
authors.forename: Ibrahim C
authors.initials: IC
authors.lastname: Haznedaroğlu
keywords: CD34+; Multiple myeloma; Progenitor cell; Renin-angiotensin system