title: Simultaneous determination of liquiritin, nodakenin and glycyrrhizinin Guibi-tang, a traditional herbal prescription by HPLC-PDA.
abstract: A simple, convenient, rapid and accurate high-performance liquid chromatographic (HPLC) method was established for the simultaneous determination about three ingredients of a traditional herbal prescription, Guibi-tang (GBT): liquiritin (1), nodakenin (2) and glycyrrhizin (3). Chromatographic analysis on the three components was separated within 35 min on a Gemini C18 column and maintained at 40°C. The mobile phase consisted of water with 1.0% (v/v) acetic acid (solvent A) and acetonitrile with 1.0% (v/v) acetic acid (solvent B) in gradient mode at a flow-rate of 1.0 mL/min. Chromatograms were acquired at 254, 280 and 330 nm in a photodiode array (PDA) detector. The calibration curves showed excellent linearity (R(2)=1.0000). The average recovery of three compounds was >93.5%, with a relative standard deviation (RSD) of <2.0%. The intra-day and inter-day precision (RSD) of the three components were 0.04%-3.04% and 0.11%-2.48%, respectively. The contents of compounds 1-3 in GBT were 1.09-1.10, 2.35-2.37 and 0.81-0.82 mg/g, respectively.
authors.affiliation: Herbal Medicine Formulation Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Chang-Seob
authors.initials: CS
authors.lastname: Seo
authors.affiliation: Herbal Medicine Formulation Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Herbal Medicine Formulation Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Hyeun-Kyoo
authors.initials: HK
authors.lastname: Shin
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D044950:Flavanones / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D019695:Glycyrrhizic Acid / Q000032:analysis*; D057230:Limit of Detection
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D019695:Glycyrrhizic Acid; C471579:nodakenin; C512196:liquiritin