title: Acetylcholinesterase inhibition by biofumigant (Coumaran) from leaves of Lantana camara in stored grain and household insect pests.
abstract: Recent studies proved that the biofumigants could be an alternative to chemical fumigants against stored grain insect pests. For this reason, it is necessary to understand the mode of action of biofumigants. In the present study the prospectus of utilising Lantana camara as a potent fumigant insecticide is being discussed. Inhibition of acetylcholinesterase (AChE) by Coumaran, an active ingredient extracted from the plant L. camara, was studied. The biofumigant was used as an enzyme inhibitor and acetylthiocholine iodide as a substrate along with Ellman's reagent to carry out the reactions. The in vivo inhibition was observed in both dose dependent and time dependent in case of housefly, and the nervous tissue (ganglion) and the whole insect homogenate of stored grain insect exposed to Coumaran. The possible mode of action of Coumaran as an acetylcholinesterase inhibitor is discussed.
authors.affiliation: Animal Bioresources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal, Manipur 795001, India.
authors.forename: Yallappa
authors.identifier: 0000-0001-6707-4643
authors.initials: Y
authors.lastname: Rajashekar
authors.affiliation: Division of Insect Ecology, National Bureau of Agriculturally Important Insects, Bangalore, Karnataka 560024, India.
authors.forename: Anjanappa
authors.initials: A
authors.lastname: Raghavendra
authors.affiliation: Division of Insect Ecology, National Bureau of Agriculturally Important Insects, Bangalore, Karnataka 560024, India.
authors.forename: Nandagopal
authors.initials: N
authors.lastname: Bakthavatsalam
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects; D000818:Animals; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D002523:Edible Grain; D007313:Insecta / Q000187:drug effects*; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D032410:Lantana / Q000737:chemistry; D010575:Pesticides / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D001572:Benzofurans; D007306:Insecticides; D010575:Pesticides; D010936:Plant Extracts; C043168:coumaran; D000110:Acetylcholinesterase