title: Derivative emission spectrofluorimetry for the simultaneous determination of guaifenesin and phenylephrine hydrochloride in pharmaceutical tablets.
abstract: Rapid, simple and sensitive derivative emission spectrofluorimetric methods have been developed for the simultaneous analysis of binary mixtures of guaifenesin (GUA) and phenylephrine hydrochloride (PHE). The methods are based upon measurement of the native fluorescence intensity of the two drugs at λex = 275 nm in methanolic solutions, followed by differentiation using first (D1) and second (D2) derivative techniques. The derivative fluorescence intensity-concentration plots were rectilinear over a range of 0.1-2 µg/mL for both GUA and PHE. The limits of detection were 0.027 (D1, GUA), 0.025 (D2, GUA), 0.031 (D1, PHE) and 0.033 (D2, PHE) µg/mL and limits of quantitation were 0.089 (D1, GUA), 0.083 (D2, GUA), 0.095 (D1, PHE) and 0.097 (D2, PHE) µg/mL. The proposed derivative emission spectrofluorimetric methods (D1 and D2) were successfully applied for the determination of the two compounds in binary mixtures and tablets with high precision and accuracy. The proposed methods were fully validated as per ICH guidelines.
authors.affiliation: College of Pharmacy, Department of Pharmaceutical Chemistry, King Saud University, P.O. Box 22452, Riyadh, 11495, Saudi Arabia; Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, University of Alexandria, El-Messalah, Alexandria, 21521, Egypt.
authors.forename: Hadir M
authors.initials: HM
authors.lastname: Maher
authors.forename: Mona M
authors.initials: MM
authors.lastname: Alshehri
authors.forename: Shorog M
authors.initials: SM
authors.lastname: Al-taweel
mesh_terms: D002138:Calibration; D006140:Guaifenesin / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D010656:Phenylephrine / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013607:Tablets / Q000032:analysis*
chemical_list: D013607:Tablets; D010656:Phenylephrine; D006140:Guaifenesin
keywords: derivative emission; guaifenesin; phenylephrine; spectrofluorimetry; tablets