title: Plasma proteins in the acquired denture pellicle enhance substrate surface free energy and Candida albicans phospholipase and proteinase activities.
abstract: OBJECTIVE
  The objective of the present study was to determine if blood plasma proteins could change the proteome of the acquired denture pellicle by label-free quantitative proteomics. As pellicle proteome modulates the interaction between substrates and Candida cells, we investigated its effect on the surface free energy (SFE) of the coated resin and on Candida albicans phospholipase and aspartyl proteinase activities.
  
  
  METHODS
  Poly(methylmethacrylate) discs were exposed to saliva (control) or saliva enriched with blood plasma (experimental group). The pellicle proteome was analyzed by mass spectrometry coupled with liquid chromatography. SFE was determined by acid-base technique. After biofilm formation, phospholipase and proteinase activities were determined accordingly to classic plate methods. Data were analyzed by two-way anova and Tukey test (P < 0.05).
  
  
  RESULTS
  α-Amylase, cystatins, mucins, and host-immune system proteins were the main proteins identified in the control group. Fibrinogen and albumin were observed only in the experimental group. Coated discs of the experimental group presented an increased SFE (P < 0.05). For both enzymes tested, the experimental group showed higher proteolytic activity (P < 0.001).
  
  
  CONCLUSIONS
  Blood plasma changes the proteome of the acquired denture pellicle, increasing surface free energy and the activity of Candida albicans phospholipase and aspartyl proteinase.
authors.affiliation: Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil.
authors.forename: William
authors.initials: W
authors.lastname: Custodio
authors.affiliation: Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil.
authors.forename: Wander J
authors.initials: WJ
authors.lastname: Silva
authors.affiliation: Brazilian Biosciences National Laboratory, Campinas, São Paulo, Brazil.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Paes Leme
authors.affiliation: Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil.
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Cury
authors.affiliation: Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil.
authors.forename: Altair A
authors.initials: AA
authors.lastname: Del Bel Cury
mesh_terms: D000328:Adult; D057055:Aspartic Acid Proteases / Q000032:analysis*; D018441:Biofilms; D001798:Blood Proteins / Q000032:analysis / Q000502:physiology*; D002176:Candida albicans / Q000201:enzymology*; D002853:Chromatography, Liquid / Q000379:methods; D015891:Cystatins / Q000032:analysis; D044622:Dental Pellicle / Q000737:chemistry / Q000502:physiology*; D003819:Denture Bases; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D007162:Immunoproteins / Q000032:analysis; D066298:In Vitro Techniques; D008297:Male; D009077:Mucins / Q000032:analysis; D010740:Phospholipases / Q000032:analysis*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D020543:Proteome / Q000378:metabolism; D011897:Random Allocation; D012709:Serum Albumin / Q000032:analysis; D013500:Surface Tension; D053719:Tandem Mass Spectrometry / Q000379:methods; D000516:alpha-Amylases / Q000032:analysis
chemical_list: D001798:Blood Proteins; D015891:Cystatins; D007162:Immunoproteins; D009077:Mucins; D020543:Proteome; D012709:Serum Albumin; D005340:Fibrinogen; D019904:Polymethyl Methacrylate; D010740:Phospholipases; D000516:alpha-Amylases; D057055:Aspartic Acid Proteases
keywords: Candida; acquired denture pellicle; plasma protein; proteome; substrate surface free energy