title: Caseins from bovine colostrum and milk strongly bind piscidin-1, an antimicrobial peptide from fish.
abstract: A model system of bovine colostrum and piscidin, a fish-derived antimicrobial peptide, was developed to study potential interactions of antimicrobial peptides in colostrum. We did not detect any antimicrobial activity of colostrum using the radial plate diffusion assay; in fact colostrum completely abrogated activity of added piscidin. This could not be explained by degradation of piscidin by colostrum, which was less than ten percent. We found that colostrum even protected piscidin against degradation by added proteases. We further observed that colostrum and milk rapidly quenched the fluorescence of fluorescein-piscidin but not that of fluorescein. This effect was not seen with BSA and the specific quenching of fluorescein-piscidin by colostrum was saturably inhibited with unlabeled piscidin. Size exclusion chromatography indicated that fluorescein-piscidin bound to casein micelles with no apparent binding to IgG or whey proteins. Further, addition of pure caseins was able to quench fluorescence of fluorescein-piscidin and to inhibit the antimicrobial activity of piscidin. The interaction between caseins and piscidin could be dissociated by guanidine hydrochloride and recovered piscidin had antimicrobial activity against bacteria. Based on our results we propose that caseins could be carriers for antimicrobial peptides in colostrum and milk.
authors.affiliation: Department of Food Science, Aarhus University, Aarhus, Tjele, Denmark; Competence Center of Food and Fermentation Technologies, Tallinn, Estonia; Department of Food Processing, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Mary-Liis
authors.initials: ML
authors.lastname: Kütt
authors.affiliation: Department of Food Science, Aarhus University, Aarhus, Tjele, Denmark. Electronic address: jan.stagsted@agrsci.dk.
authors.forename: Jan
authors.initials: J
authors.lastname: Stagsted
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002364:Caseins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002417:Cattle; D003126:Colostrum / Q000737:chemistry*; D029941:Fish Proteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D005399:Fishes; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000737:chemistry*; D008970:Molecular Weight; D011485:Protein Binding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D002364:Caseins; D029941:Fish Proteins; C449802:moronecidin protein, Morone saxatilis
keywords: Antimicrobial peptides; Caseins; Colostrum; Milk; Piscidin