title: Determination of selected parameters for non-specific and specific immunity in cows with subclinical endometritis.
abstract: Endometritis in dairy cow herds is a serious economic problem all over the world due to the large economic losses. The aim of the study was a comparative evaluation of selected indicators of non-specific and specific immunity in cows with subclinical endometritis and in cows without inflammation of the uterus. The study was performed on 40 cows on day 65 after delivery. Based on the results of cytological tests, the cows were divided into two groups: experimental (subclinical endometritis) and control (20 cows in each group). A flow cytometric analysis was performed for the leukocyte surface molecules CD4, CD8, CD14, CD21, CD25. Moreover the phagocytic activity of granulocytes and monocytes/macrophages in peripheral blood and uterine washings was determined. It has been demonstrated that the percentage of phagocytic granulocytes and monocytes/macrophages in both the peripheral blood and uterine washings was significantly lower for cows with subclinical endometritis when compared to cows undergoing a normal puerperal period (p<0.001). A significant (p≤0.001) decrease in the percentage of CD4+, CD14+, CD25+ and CD4+CD25+ leukocytes was also observed in peripheral blood of the cows from the experimental group. In uterine washings a significant decrease (p<0.001) in CD21+ and increase in CD8+ lymphocytes was detected. The results indicate that dysfunction of cell immunity coexisting with subclinical endometritis may be the main factor causing advanced inflammation of the uterus. Knowledge of immunological mechanisms observed in cows with subclinical endometritis could aid in choosing the right adjuvant therapy using immunomodulating agents.
authors.affiliation: Department and Clinic of Reproduction, University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland. Electronic address: wetdoc@interia.pl.
authors.forename: P
authors.initials: P
authors.lastname: Brodzki
authors.affiliation: Department of Epizootiology and Clinic of Infections Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-033 Lublin, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Kostro
authors.affiliation: Department and Clinic of Animal Surgery, University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Brodzki
authors.affiliation: Department of Epizootiology and Clinic of Infections Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-033 Lublin, Poland.
authors.forename: U
authors.initials: U
authors.lastname: Lisiecka
mesh_terms: D000818:Animals; D058070:Asymptomatic Diseases; D015415:Biomarkers / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D001769:Blood / Q000378:metabolism; D001826:Body Fluids / Q000378:metabolism; D016022:Case-Control Studies; D002417:Cattle / Q000276:immunology; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism; D004716:Endometritis / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism; D005260:Female; D007109:Immunity / Q000502:physiology*; D008828:Microbiological Techniques; D011379:Prognosis; D014599:Uterus / Q000276:immunology / Q000378:metabolism / Q000382:microbiology
chemical_list: D015415:Biomarkers
keywords: Cytology; Phagocytosis; Subclinical endometritis; Subpopulations leukocytes