title: FANCM-associated proteins MHF1 and MHF2, but not the other Fanconi anemia factors, limit meiotic crossovers.
abstract: Genetic recombination is important for generating diversity and to ensure faithful segregation of chromosomes at meiosis. However, few crossovers (COs) are formed per meiosis despite an excess of DNA double-strand break precursors. This reflects the existence of active mechanisms that limit CO formation. We previously showed that AtFANCM is a meiotic anti-CO factor. The same genetic screen now identified AtMHF2 as another player of the same anti-CO pathway. FANCM and MHF2 are both Fanconi Anemia (FA) associated proteins, prompting us to test the other FA genes conserved in Arabidopsis for a role in CO control at meiosis. This revealed that among the FA proteins tested, only FANCM and its two DNA-binding co-factors MHF1 and MHF2 limit CO formation at meiosis.
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR1318, ERL CNRS 3559,Saclay Plant Sciences, RD10, 78000 Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences,RD10, 78000 Versailles, France.
authors.forename: Chloe
authors.initials: C
authors.lastname: Girard
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR1318, ERL CNRS 3559,Saclay Plant Sciences, RD10, 78000 Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences,RD10, 78000 Versailles, France.
authors.forename: Wayne
authors.initials: W
authors.lastname: Crismani
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR1318, ERL CNRS 3559,Saclay Plant Sciences, RD10, 78000 Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences,RD10, 78000 Versailles, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Froger
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR1318, ERL CNRS 3559,Saclay Plant Sciences, RD10, 78000 Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences,RD10, 78000 Versailles, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Mazel
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR1318, ERL CNRS 3559,Saclay Plant Sciences, RD10, 78000 Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences,RD10, 78000 Versailles, France.
authors.forename: Afef
authors.initials: A
authors.lastname: Lemhemdi
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR1318, ERL CNRS 3559,Saclay Plant Sciences, RD10, 78000 Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences,RD10, 78000 Versailles, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Horlow
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, UMR1318, ERL CNRS 3559,Saclay Plant Sciences, RD10, 78000 Versailles, France AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, ERL CNRS 3559, Saclay Plant Sciences,RD10, 78000 Versailles, France raphael.mercier@versailles.inra.fr.
authors.forename: Raphael
authors.initials: R
authors.lastname: Mercier
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D004265:DNA Helicases / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D051856:Fanconi Anemia Complementation Group Proteins / Q000235:genetics / Q000502:physiology*; D008540:Meiosis / Q000235:genetics*; D009154:Mutation; D011995:Recombination, Genetic
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D051856:Fanconi Anemia Complementation Group Proteins; C000592802:MHF1 protein, Arabidopsis; C000592803:MHF2 protein, Arabidopsis; D004265:DNA Helicases; C573664:FANCM protein, Arabidopsis