title: Preparation and antibacterial activity of the oligosaccharides derived from Rhizoma Phragmites.
abstract: In this study, we prepared Rhizoma Phragmites derived oligosaccharides (ROs) by hydrolysis with hydrogen peroxide (H2O2). The ROs yield was affected by reaction time, temperature, and H2O2 concentration. Too long reaction time and too high temperature decreased the ROs yield. Maximum ROs yield (11.26%) was obtained at reaction time 4h, temperature 75 °C, and H2O2 concentration 3.5% (v/v). The oligosaccharides sample contained 93.16% sugar, of which the average degree was approximately 11, was water-soluble, and showed white. The ROs had the highest antibacterial activity against Staphylococcus aureus, followed by Bacillus subtilis and Escherichia coli at the concentration of 100 μg/mL.
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China. Electronic address: qianzhiganghu@sina.cn.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Qian
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China.
authors.forename: Long-Fa
authors.initials: LF
authors.lastname: Jiang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000737:chemistry; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D006861:Hydrogen Peroxide
keywords: Antibacterial activity; Hydrogen peroxide; Rhizoma Phragmites derived oligosaccharides