title: Upregulated expression of SSTR1 is involved in neuronal apoptosis and is coupled to the reduction of bcl-2 following intracerebral hemorrhage in adult rats.
abstract: Somatostatins are peptide hormones that regulate diverse cellular processes, such as neurotransmission, cell proliferation, apoptosis, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. SSTR1 is a member of the superfamily of somatostatin receptors possessing seven-transmembrane segments. Aberrant expression of SSTR1 has been implicated in several human diseases, including pseudotumor cerebri, and oncogenic osteomalacia. In this study, we investigated a potential role of SSTR1 in the regulation of neuronal apoptosis in the course of intracerebral hemorrhage (ICH). A rat ICH model in the caudate putamen was established and subjected to behavioral tests. Western blot and immunohistochemistry indicated a remarkable up-regulation of SSTR1 expression surrounding the hematoma after ICH. Double-labeled immunofluorescence showed that SSTR1 was mostly co-localized with neurons, and was rarely distributed in activated astrocytes and microglia. Additionally, SSTR1 co-localized with active-caspase-3 and bcl-2 around the hematoma. The expression of active-caspase-3 was parallel with that of SSTR1 in a time-dependent manner. In addition, SSTR1 knockdown specifically resulted in reduced neuronal apoptosis in PC12 cells. All our findings suggested that up-regulated SSTR1 contributed to neuronal apoptosis after ICH, which was accompanied with reduced expression of bcl-2.
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Immunology, Medical College, Nantong University, Nantong, 226001, People's Republic of China.
authors.forename: Damin
authors.initials: D
authors.lastname: Yuan
authors.forename: Jianhong
authors.initials: J
authors.lastname: Shen
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Yan
authors.forename: Xinmin
authors.initials: X
authors.lastname: Wu
authors.forename: Aihong
authors.initials: A
authors.lastname: Li
authors.forename: Aisong
authors.initials: A
authors.lastname: Guo
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wu
authors.forename: Chengwei
authors.initials: C
authors.lastname: Duan
authors.forename: Jiabing
authors.initials: J
authors.lastname: Shen
authors.forename: Cuiying
authors.initials: C
authors.lastname: Tang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Ji
mesh_terms: D000375:Aging / Q000473:pathology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006406:Hematoma / Q000378:metabolism / Q000473:pathology; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017481:Receptors, Somatostatin / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D015415:Biomarkers; D019253:Proto-Oncogene Proteins c-bcl-2; D017481:Receptors, Somatostatin; C101130:somatostatin receptor type 1; D006427:Hemin; D053148:Caspase 3