title: Interrogation of in vivo protein-protein interactions using transgenic mouse models and stable isotope labeling.
abstract: Methods in mass spectrometry have evolved in recent years, facilitating proteomic analyses that were previously beyond the limits of the technology. Transgenic mouse models, coupled with mass spectrometry proteomics, have served as valuable platform for elucidating the in vivo function of individual genes and proteins. Here we discuss the methods we have recently employed to characterize protein-protein interactions and posttranslational modifications in tagged knock-in mouse models. These methods can be broadly applied to other systems for various applications in both basic and translational science.
authors.affiliation: Department of Molecular Biology, Genentech, Inc., 1 DNA Way, South San Francisco, CA, 94080, USA, dey.anwesha@gene.com.
authors.forename: Anwesha
authors.initials: A
authors.lastname: Dey
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Wu
authors.forename: Donald S
authors.initials: DS
authors.lastname: Kirkpatrick
mesh_terms: D000818:Animals; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D025941:Protein Interaction Mapping / Q000379:methods*; D040901:Proteomics / Q000379:methods