title: Binding of Aβ peptide creates lipid density depression in DMPC bilayer.
abstract: Using isobaric-isothermal replica exchange molecular dynamics and all-atom explicit water model we study the impact of Aβ monomer binding on the equilibrium properties of DMPC bilayer. We found that partial insertion of Aβ peptide into the bilayer reduces the density of lipids in the binding "footprint" and indents the bilayer thus creating a lipid density depression. Our simulations also reveal thinning of the bilayer and a decrease in the area per lipid in the proximity of Aβ. Although structural analysis of lipid hydrophobic core detects disordering in the orientations of lipid tails, it also shows surprisingly minor structural perturbations in the tail conformations. Finally, partial insertion of Aβ monomer does not enhance water permeation through the DMPC bilayer and even causes considerable dehydration of the lipid-water interface. Therefore, we conclude that Aβ monomer bound to the DMPC bilayer fails to perturb the bilayer structure in both leaflets. Limited scope of structural perturbations in the DMPC bilayer caused by Aβ monomer may constitute the molecular basis of its low cytotoxicity.
authors.affiliation: School of Systems Biology, George Mason University, Manassas, VA 20110, United States.
authors.forename: Christopher
authors.initials: C
authors.lastname: Lockhart
authors.affiliation: School of Systems Biology, George Mason University, Manassas, VA 20110, United States. Electronic address: dklimov@gmu.edu.
authors.forename: Dmitri K
authors.initials: DK
authors.lastname: Klimov
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D008051:Lipid Bilayers; D004134:Dimyristoylphosphatidylcholine
keywords: Alzheimer's disease; Aβ peptide; DMPC lipid bilayer; Isobaric–isothermal replica exchange; Molecular dynamics