title: Measuring NAD(+) levels in mouse blood and tissue samples via a surrogate matrix approach using LC-MS/MS.
abstract: BACKGROUND
  NAD(+) is an endogenous analyte and is unstable during blood sample collection, both of which present obstacles for quantitation. Moreover, current procedures for NAD(+) sample collection require onsite treatment with strong acid to stabilize the NAD(+) in mouse blood cells.
  
  
  RESULTS
  NAD(+) can be stabilized by addition of acid before the frozen mouse blood sample was thawed. A simple sample collection procedure was proposed to facilitate the analysis of NAD(+) in mouse blood and tissue samples. A LC-MS/MS method was developed for quantifying NAD(+) in mouse blood and various tissue samples. The described method was used to measure endogenous NAD(+) levels in mouse blood following oral administration of the nicotinamide phosphoribosyltransferase inhibitor GNE-617.
  
  
  CONCLUSIONS
  This study presents a suitable assay and sample collection procedure for high throughput screening of NAD(+) samples in preclinical discovery studies.
authors.affiliation: Drug Metabolism & Pharmacokinetics, Genentech Inc., South San Francisco, CA 94080, USA.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Liang
authors.forename: Lulu
authors.initials: L
authors.lastname: Yang
authors.forename: Ann R
authors.initials: AR
authors.lastname: Qin
authors.forename: Justin
authors.initials: J
authors.lastname: Ly
authors.forename: Bianca M
authors.initials: BM
authors.lastname: Liederer
authors.forename: Kirsten
authors.initials: K
authors.lastname: Messick
authors.forename: Shuguang
authors.initials: S
authors.lastname: Ma
authors.forename: Mark
authors.initials: M
authors.lastname: Zak
authors.forename: Peter S
authors.initials: PS
authors.lastname: Dragovich
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Dean
authors.forename: Cornelis E C A
authors.initials: CE
authors.lastname: Hop
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Deng
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D009243:NAD / Q000097:blood*
chemical_list: D009243:NAD