title: Contribution of aberrant GluK2-containing kainate receptors to chronic seizures in temporal lobe epilepsy.
abstract: Kainate is a potent neurotoxin known to induce acute seizures. However, whether kainate receptors (KARs) play any role in the pathophysiology of temporal lobe epilepsy (TLE) is not known. In TLE, recurrent mossy fiber (rMF) axons form abnormal excitatory synapses onto other dentate granule cells that operate via KARs. The present study explores the pathophysiological implications of KARs in generating recurrent seizures in chronic epilepsy. In an in vitro model of TLE, seizure-like activity was minimized in mice lacking the GluK2 subunit, which is a main component of aberrant synaptic KARs at rMF synapses. In vivo, the frequency of interictal spikes and ictal discharges was strongly reduced in GluK2(-/-) mice or in the presence of a GluK2/GluK5 receptor antagonist. Our data show that aberrant GluK2-containing KARs play a major role in the chronic seizures that characterize TLE and thus constitute a promising antiepileptic target.
authors.affiliation: INSERM, INMED, U901, 13009 Marseille, France; Aix-Marseille Université, UMR 901, 13009 Marseille, France.
authors.forename: Angélique
authors.initials: A
authors.lastname: Peret
authors.affiliation: INSERM, INMED, U901, 13009 Marseille, France; Aix-Marseille Université, UMR 901, 13009 Marseille, France.
authors.forename: Louisa A
authors.initials: LA
authors.lastname: Christie
authors.affiliation: INSERM, INMED, U901, 13009 Marseille, France; Aix-Marseille Université, UMR 901, 13009 Marseille, France.
authors.forename: David W
authors.initials: DW
authors.lastname: Ouedraogo
authors.affiliation: Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, University of Bordeaux, 33000 Bordeaux, France.
authors.forename: Adam
authors.initials: A
authors.lastname: Gorlewicz
authors.affiliation: INSERM, INMED, U901, 13009 Marseille, France; Aix-Marseille Université, UMR 901, 13009 Marseille, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Epsztein
authors.affiliation: Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, University of Bordeaux, 33000 Bordeaux, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Mulle
authors.affiliation: INSERM, INMED, U901, 13009 Marseille, France; Aix-Marseille Université, UMR 901, 13009 Marseille, France. Electronic address: valerie.crepel@inserm.fr.
authors.forename: Valérie
authors.initials: V
authors.lastname: Crépel
mesh_terms: D000818:Animals; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism* / Q000503:physiopathology; D019706:Excitatory Postsynaptic Potentials; D008297:Male; D051379:Mice; D019599:Mossy Fibers, Hippocampal / Q000378:metabolism / Q000502:physiology*; D018092:Kainic Acid Receptors / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D012640:Seizures / Q000378:metabolism* / Q000503:physiopathology; D000097806:GluK2 Kainate Receptor
chemical_list: D018092:Kainic Acid Receptors; D000097806:GluK2 Kainate Receptor