title: The mouse intranasal challenge model for potency testing of whole-cell pertussis vaccines.
abstract: BACKGROUND
  A mouse intracerebral challenge model is used for potency testing of whole-cell pertussis (wP) vaccines. We investigated the use of a mouse nasopharyngeal challenge model, which better reflects the clinical features of pertussis disease, to differentiate between efficacy of wP vaccines.
  
  
  METHODS
  Efficacy of three wP vaccines (Quinvaxem(®), Easyfive(®) and Pentavac(®)) was tested in the nasopharyngeal challenge model. Mice were vaccinated at 4 and 7 weeks and challenged with Bordetella pertussis at 9 weeks. Vaccine efficacy was determined based on CFU in the lungs 5 days after challenge.
  
  
  RESULTS
  The mouse nasopharyngeal challenge model has the capacity to differentiate between the efficacy of whole cell pertussis vaccines.
  
  
  CONCLUSIONS
  The mouse nasopharyngeal challenge model could be considered as a potency and release assay for wP vaccines. Whether this model directly correlates with clinical vaccine efficacy requires further investigations. Whether this model directly correlates with clinical vaccine efficacy requires further investigations. The mouse nasopharyngeal challenge model could be considered as a potency and release assay for wP vaccines.
authors.affiliation: Janssen Pharmaceutical Research and Development - Bacterial Vaccines Research and Development, 600 Welsh and McKean Rd, Spring House, Pennsylvania, USA.
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Queenan
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Fernandez
authors.forename: Wenchi
authors.initials: W
authors.lastname: Shang
authors.forename: Selma
authors.initials: S
authors.lastname: Wiertsema
authors.forename: Germie P J M
authors.initials: GP
authors.lastname: van den Dobbelsteen
authors.forename: Jan
authors.initials: J
authors.lastname: Poolman
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D001886:Bordetella pertussis / Q000276:immunology; D003114:Colony-Forming Units Assay; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000276:immunology*; D014917:Whooping Cough / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D010567:Pertussis Vaccine
keywords: Bordetella pertussis; Kendrick test; mouse nasopharyngeal challenge model; vaccine release assay; whole cell pertussis vaccine