title: Improved ethanol production from cheese whey, whey powder, and sugar beet molasses by "Vitreoscilla hemoglobin expressing" Escherichia coli.
abstract: This work investigated the improvement of ethanol production by engineered ethanologenic Escherichia coli to express the hemoglobin from the bacterium Vitreoscilla (VHb). Ethanologenic E. coli strain FBR5 and FBR5 transformed with the VHb gene in two constructs (strains TS3 and TS4) were grown in cheese whey (CW) medium at small and large scales, at both high and low aeration, or with whey powder (WP) or sugar beet molasses hydrolysate (SBMH) media at large scale and low aeration. Culture pH, cell growth, VHb levels, and ethanol production were evaluated after 48 h. VHb expression in TS3 and TS4 enhanced their ethanol production in CW (21-419%), in WP (17-362%), or in SBMH (48-118%) media. This work extends the findings that "VHb technology" may be useful for improving the production of ethanol from waste and byproducts of various sources.
authors.affiliation: a Department of Molecular Biology and Genetics , Gebze Institute of Technology , Gebze , Turkey.
authors.forename: Meltem Yesilcimen
authors.initials: MY
authors.lastname: Akbas
authors.forename: Taner
authors.initials: T
authors.lastname: Sar
authors.forename: Busra
authors.initials: B
authors.lastname: Ozcelik
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D027461:Beta vulgaris / Q000737:chemistry*; D019149:Bioreactors / Q000382:microbiology; D002611:Cheese; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005818:Genetic Engineering; D008965:Molasses; D014170:Transformation, Genetic; D054793:Truncated Hemoglobins / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D000431:Ethanol
keywords: Escherichia coli; Vitreoscilla hemoglobin; bioethanol; sugar beet molasses; whey