title: PIKfyve inhibition interferes with phagosome and endosome maturation in macrophages.
abstract: Macrophages eliminate pathogens and cell debris through phagocytosis, a process by which particulate matter is engulfed and sequestered into a phagosome. Nascent phagosomes are innocuous organelles resembling the plasma membrane. However, through a maturation process, phagosomes are quickly remodeled by fusion with endosomes and lysosomes to form the phagolysosome. Phagolysosomes are highly acidic and degradative leading to particle decomposition. Phagosome maturation is intimately dependent on the endosomal pathway, during which diverse cargoes are sorted for recycling to the plasma membrane or for degradation in lysosomes. Not surprisingly, various regulators of the endosomal pathway are also required for phagosome maturation, including phosphatidylinositol-3-phosphate, an early endosomal regulator. However, phosphatidylinositol-3-phosphate can be modified by the lipid kinase PIKfyve into phosphatidylinositol-3,5-bisphosphate, which controls late endosome/lysosome functions. The role of phosphatidylinositol-3,5-bisphosphate in macrophages and phagosome maturation remains basically unexplored. Using Fcγ receptor-mediated phagocytosis as a model, we describe our research showing that inhibition of PIKfyve hindered certain steps of phagosome maturation. In particular, PIKfyve antagonists delayed removal of phosphatidylinositol-3-phosphate and reduced acquisition of LAMP1 and cathepsin D, both common lysosomal proteins. Consistent with this, the degradative capacity of phagosomes was reduced but phagosomes appeared to still acidify. We also showed that trafficking to lysosomes and their degradative capacity was reduced by PIKfyve inhibition. Overall, we provide evidence that PIKfyve, likely through phosphatidylinositol-3,5-bisphosphate synthesis, plays a significant role in endolysosomal and phagosome maturation in macrophages.
authors.affiliation: Deparment of Chemistry and Biology and the Molecular Science Program, Ryerson University, Toronto, Ontario, M5B2K3, Canada.
authors.forename: Grace H E
authors.initials: GH
authors.lastname: Kim
authors.forename: Roya M
authors.initials: RM
authors.lastname: Dayam
authors.forename: Akriti
authors.initials: A
authors.lastname: Prashar
authors.forename: Mauricio
authors.initials: M
authors.lastname: Terebiznik
authors.forename: Roberto J
authors.initials: RJ
authors.lastname: Botelho
mesh_terms: D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D002402:Cathepsin D / Q000378:metabolism; D002460:Cell Line; D011992:Endosomes / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006575:Heterocyclic Compounds, 3-Ring / Q000494:pharmacology; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D021381:Protein Transport; D017452:Receptors, IgG / Q000378:metabolism
chemical_list: D000631:Aminopyridines; D002402:Cathepsin D; D004791:Enzyme Inhibitors; D006575:Heterocyclic Compounds, 3-Ring; D052118:Lysosomal-Associated Membrane Protein 1; D019869:Phosphatidylinositol 3-Kinases; D018129:Phosphatidylinositol Phosphates; D000081082:Phosphoinositide-3 Kinase Inhibitors; D017452:Receptors, IgG; C540576:6-amino-N-(3-(4-(4-morpholinyl)pyrido(3',2'-4,5)furo(3,2-d)pyrimidin-2-yl)phenyl)-3-pyridinecarboxamide; C055525:phosphatidylinositol 3-phosphate
keywords: endosome; lysosomes; macrophages; organelles; phagocytosis; phagosome maturation; phosphoinositides