title: Development of a porcine renal extracellular matrix scaffold as a platform for kidney regeneration.
abstract: Acellular scaffolds, possessing an intact three-dimensional extracellular matrix (ECM) architecture and biochemical components, are promising for regeneration of complex organs, such as the kidney. We have successfully developed a porcine renal acellular scaffold and analyzed its physical/biochemical characteristics, biocompatibility, and kidney reconstructive potential. Segmented porcine kidney cortexes were treated with either 1% (v/v) Triton X-100 (Triton) or sodium dodecyl sulfate (SDS). Scanning electron microscopy showed both treatments preserved native tissue architecture, including porosity and composition. Swelling behavior was higher in the Triton-treated compared with the SDS-treated scaffold. Maximum compressive strength was lower in the Triton-treated compared with the SDS-treated scaffold. Attenuated total reflective-infrared spectroscopy showed the presence of amide II (-NH) in both scaffolds. Furthermore, richer ECM protein and growth factor contents were observed in the Triton-treated compared with SDS-treated scaffold. Primary human kidney cell adherence, viability, and proliferation were enhanced on the Triton-treated scaffold compared with SDS-treated scaffold. Following murine in vivo implantation, tumorigenecity was absent for both scaffolds after 8 weeks and in the Triton-treated scaffold only, glomeruli-like structure formation and neovascularity were observed. We identified 1% Triton X-100 as a more suitable decellularizing agent for porcine renal ECM scaffolds prior to kidney regeneration.
authors.affiliation: Department of Urology, School of Medicine, Kyungpook National University, Daegu, Korea.
authors.forename: Seock Hwan
authors.initials: SH
authors.lastname: Choi
authors.forename: So Young
authors.initials: SY
authors.lastname: Chun
authors.forename: Seon Yeong
authors.initials: SY
authors.lastname: Chae
authors.forename: Jin Rae
authors.initials: JR
authors.lastname: Kim
authors.forename: Se Heang
authors.initials: SH
authors.lastname: Oh
authors.forename: Sung Kwang
authors.initials: SK
authors.lastname: Chung
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Phil Hyun
authors.initials: PH
authors.lastname: Song
authors.forename: Gyu-Seog
authors.initials: GS
authors.lastname: Choi
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Kim
authors.forename: Tae Gyun
authors.initials: TG
authors.lastname: Kwon
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D063646:Carcinogenesis / Q000187:drug effects / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D051379:Mice; D017830:Octoxynol / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D034421:Sus scrofa; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D003902:Detergents; D016326:Extracellular Matrix Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol; D004247:DNA
keywords: Triton X-100; extracellular matrix scaffold; kidney reconstruction; partial nephrectomy; sodium dodecyl sulfate