title: MiR-126 inhibits the invasion of gastric cancer cell in part by targeting Crk.
abstract: OBJECTIVE
  Accumulating evidence has shown that microRNAs (miRNAs) are aberrantly expressed in human gastric cancer and crucial to tumorigenesis. Herein, we identified the role of miR-126 in human gastric cancer (GC) growth and development in vitro.
  
  
  METHODS
  MiR-126 expression was investigated in GC tissue samples and cell lines by real-time PCR. Crystal violet test and Transwell assay were conducted to explore the effects of miR-126 on the proliferation and invasion of human GC cell lines, respectively. The impaction of miR-126 over expression on putative target Crk (v-crk sarcoma virus CT10 oncogene homolog) was subsequently confirmed via Western blot. Crk specific siRNA was used to suppress Crk expression.
  
  
  RESULTS
  MiR-126 expression was frequently and markedly downregulated (p < 0.05) in human gastric cancer tissues. Overexpression of miR-126 inhibited GC cells invasion but did not affect its proliferation in vitro. Moreover, overexpression of miR-126 significantly decreased (p < 0.05) the protein levels of Crk, which has previously been identified as a direct target of miR-126. Knockdown of Crk also markedly suppressed GC cells invasion.
  
  
  CONCLUSIONS
  Our results demonstrated that overexpression of miR-126 inhibited GC cells invasion in part by targeting Crk. These findings suggested that miR-126 played major roles in the malignant behavior of GC and it might be a promising therapeutic target of GC.
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China. qiyu2002@yahoo.com.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Qi
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D050737:Proto-Oncogene Proteins c-crk / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C485285:CRK protein, human; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D050737:Proto-Oncogene Proteins c-crk; D034741:RNA, Small Interfering