title: Assessment of a portable prothrombin time analyzer (CoaguChek-XS) in dogs.
abstract: OBJECTIVE
  To assess the performance of a portable prothrombin time (PT) analyzer (CoaguChek-XS) in dogs.
  
  
  METHODS
  Ninety-seven dogs composed of 20 healthy dogs, 45 ill dogs, and 32 ill dogs with altered coagulation.
  
  
  METHODS
  Samples were concurrently obtained and PT was measured using the CoaguChek-XS and an automated coagulation analyzer. Agreement between methods was assessed using Spearman's correlation and Bland-Altman analysis.
  
  
  RESULTS
  The reference interval for PT using the CoaguChek-XS was 9.6-11.5 s. The CoaguChek-XS was easy to use, required a small volume of whole blood, and gave results within 1 min. The mean difference in PT between the 2 methods was 2.58 s (SD 3.10), and 94% of the samples fell within limits of agreement. The correlation was moderate, but significant (r = 0.35, P < 0.001). Sensitivity and specificity of the CoaguCheck-XS PT compared to analyzer PT were 92% and 56%, respectively, and increased to 95% and 77% on intent to treat basis. The coefficient of variance was 0.72%. The CoaguChek-XS identified all 8 dogs with anticoagulant rodenticide intoxication, although a discrepancy was noted from the analyzer results. In anemic dogs (PCV≤25%), the CoaguChek-XS did not display accurate results.
  
  
  CONCLUSIONS
  The CoaguChek-XS is a simple, user-friendly, highly precise PT analyzer. Results had moderate correlation and good agreement with a standard method. It can be used reliably for screening dogs when the PT is normal. However, when the CoaguChek-XS PT is prolonged or when the PCV is ≤25%, results should be confirmed using a standard method.
authors.affiliation: Department of Small Animal Emergency and Critical Care, the Hebrew University Veterinary Teaching Hospital, Koret School of Veterinary Medicine, Rehovot, Israel.
authors.forename: Efrat
authors.initials: E
authors.lastname: Kelmer
authors.forename: Gilad
authors.initials: G
authors.lastname: Segev
authors.forename: Carolina
authors.initials: C
authors.lastname: Codner
authors.forename: Yaron
authors.initials: Y
authors.lastname: Bruchim
authors.forename: Sigal
authors.initials: S
authors.lastname: Klainbart
authors.forename: Itamar
authors.initials: I
authors.lastname: Aroch
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage; D001777:Blood Coagulation / Q000187:drug effects*; D004283:Dog Diseases / Q000097:blood / Q000188:drug therapy*; D004285:Dogs; D005260:Female; D019934:International Normalized Ratio / Q000295:instrumentation / Q000662:veterinary*; D008297:Male; D019095:Point-of-Care Systems; D011517:Prothrombin Time / Q000295:instrumentation / Q000662:veterinary*; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000925:Anticoagulants
keywords: coagulation testing; coagulopathy; point-of-care testing