title: Analysis of gene-disruption mutants of a sucrose phosphate synthase gene in rice, OsSPS1, shows the importance of sucrose synthesis in pollen germination.
abstract: The molecular function of an isoform of sucrose phosphate synthase (SPS) in rice, OsSPS1, was investigated using gene-disruption mutant lines generated by retrotransposon insertion. The progeny of the heterozygote of disrupted OsSPS1 (SPS1(+/-)) segregated into SPS1(+/+), SPS1(+/-), and SPS1(-/-) at a ratio of 1:1:0. This distorted segregation ratio, together with the expression of OsSPS1 in the developing pollen revealed by quantitative RT-PCR analysis and promoter-beta-glucuronidase (GUS) fusion assay, suggested that the disruption of OsSPS1 results in sterile pollen. This hypothesis was reinforced by reciprocal crosses of SPS1(+/-) plants with wild-type plants in which the disrupted OsSPS1 was not paternally transmitted to the progeny. While the pollen grains of SPS(+/-) plants normally accumulated starch during their development, pollen germination on the artificial media was reduced to half of that observed in the wild-type control. Overall, our data suggests that sucrose synthesis via OsSPS1 is essential in pollen germination in rice.
authors.affiliation: NARO Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan; Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: dragon@affrc.go.jp.
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Hirose
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Hashida
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Aoki
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Okamura
authors.affiliation: Bio Research Laboratory, Toyota Motor Corporation, 1 Toyota-cho, Toyota, Aichi 471-8572, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Yonekura
authors.affiliation: Bio Research Laboratory, Toyota Motor Corporation, 1 Toyota-cho, Toyota, Aichi 471-8572, Japan.
authors.forename: Chikara
authors.initials: C
authors.lastname: Ohto
authors.affiliation: NARO Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan.
authors.forename: Tomio
authors.initials: T
authors.lastname: Terao
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Ryu
authors.initials: R
authors.lastname: Ohsugi
mesh_terms: D003433:Crosses, Genetic; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D006579:Heterozygote; D009154:Mutation; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011058:Pollen / Q000378:metabolism*; D018626:Retroelements; D013213:Starch / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D018626:Retroelements; D013395:Sucrose; D013213:Starch; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase
keywords: Oryza sativa L; Pollen germination; Pollen maturation; Sucrose phosphate synthase