title: Semi-automated protocol for purification of Mycobacterium leprae from tissues using the gentleMACS™ Octo Dissociator.
abstract: Mycobacterium leprae, etiologic agent of leprosy, is propagated in athymic nude mouse footpads (FPs). The current purification protocol is tedious and physically demanding. A simpler, semi-automated protocol was developed using gentleMACS™ Octo Dissociator. The gentleMACS protocol provided a very effective means for purification of highly viable M. leprae from tissue.
authors.affiliation: Department of Health and Human Services, Health Resource Services Administration, Health Systems Bureau, National Hansen's Disease Programs, Laboratory Research Branch, SVM-LSU, RM 3517W, Skip Bertman Dr., Baton Rouge, LA 70803, USA. Electronic address: dwilliams2@hrsa.gov.
authors.forename: Diana L
authors.initials: DL
authors.lastname: Williams
authors.affiliation: Department of Health and Human Services, Health Resource Services Administration, Health Systems Bureau, National Hansen's Disease Programs, Laboratory Research Branch, SVM-LSU, RM 3517W, Skip Bertman Dr., Baton Rouge, LA 70803, USA.
authors.forename: Linda B
authors.initials: LB
authors.lastname: Adams
authors.affiliation: Department of Health and Human Services, Health Resource Services Administration, Health Systems Bureau, National Hansen's Disease Programs, Laboratory Research Branch, SVM-LSU, RM 3517W, Skip Bertman Dr., Baton Rouge, LA 70803, USA.
authors.forename: Ramanuj
authors.initials: R
authors.lastname: Lahiri
mesh_terms: D000818:Animals; D057205:Automation, Laboratory / Q000379:methods*; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D008819:Mice, Nude; D009166:Mycobacterium leprae / Q000302:isolation & purification*; D010336:Pathology / Q000379:methods*
keywords: Leprosy; Mouse footpad tissue; Mycobacterium leprae; Purification; gentleMACS Dissociator