title: RNAi-mediated pinoresinol lariciresinol reductase gene silencing in flax (Linum usitatissimum L.) seed coat: consequences on lignans and neolignans accumulation.
abstract: RNAi technology was applied to down regulate LuPLR1 gene expression in flax (Linum usitatissimum L.) seeds. This gene encodes a pinoresinol lariciresinol reductase responsible for the synthesis of (+)-secoisolariciresinol diglucoside (SDG), the major lignan accumulated in the seed coat. If flax lignans biological properties and health benefits are well documented their roles in planta remain unclear. This loss of function strategy was developed to better understand the implication of the PLR1 enzyme in the lignan biosynthetic pathway and to provide new insights on the functions of these compounds. RNAi plants generated exhibited LuPLR1 gene silencing as demonstrated by quantitative RT-PCR experiments and the failed to accumulate SDG. The accumulation of pinoresinol the substrate of the PLR1 enzyme under its diglucosylated form (PDG) was increased in transgenic seeds but did not compensate the overall loss of SDG. The monolignol flux was also deviated through the synthesis of 8-5' linked neolignans dehydrodiconiferyl alcohol glucoside (DCG) and dihydro-dehydrodiconiferyl alcohol glucoside (DDCG) which were observed for the first time in flax seeds.
authors.affiliation: Laboratoire LBLGC EA 1207, Antenne Scientifique Universitaire de Chartres Univ Orleans, 21 rue de Loigny la Bataille, 28000 Chartres, France. Electronic address: sullivan.renouard@univ-orleans.fr.
authors.forename: Sullivan
authors.initials: S
authors.lastname: Renouard
authors.affiliation: Laboratoire BIOPI EA 3900, UFR de Pharmacie, 1 rue des Louvels 80037 Amiens, France.
authors.forename: Marie-Aude
authors.initials: MA
authors.lastname: Tribalatc
authors.affiliation: Laboratoire LBLGC EA 1207, Antenne Scientifique Universitaire de Chartres Univ Orleans, 21 rue de Loigny la Bataille, 28000 Chartres, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Lamblin
authors.affiliation: CRRBM, Univ Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Mongelard
authors.affiliation: Laboratoire BIOPI EA 3900, UFR de Pharmacie, 1 rue des Louvels 80037 Amiens, France.
authors.forename: Ophélie
authors.initials: O
authors.lastname: Fliniaux
authors.affiliation: Laboratoire LBLGC EA 1207, Antenne Scientifique Universitaire de Chartres Univ Orleans, 21 rue de Loigny la Bataille, 28000 Chartres, France.
authors.forename: Cyrielle
authors.initials: C
authors.lastname: Corbin
authors.affiliation: Laboratoire LBLGC EA 1207, Antenne Scientifique Universitaire de Chartres Univ Orleans, 21 rue de Loigny la Bataille, 28000 Chartres, France.
authors.forename: Djurdjica
authors.initials: D
authors.lastname: Marosevic
authors.affiliation: Plateforme analytique, UFR Sciences, 80039 Amiens, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Pilard
authors.affiliation: CRRBM, Univ Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Demailly
authors.affiliation: CRRBM, Univ Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Gutierrez
authors.affiliation: Laboratoire LBLGC EA 1207, Antenne Scientifique Universitaire de Chartres Univ Orleans, 21 rue de Loigny la Bataille, 28000 Chartres, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hano
authors.affiliation: Laboratoire BIOPI EA 3900, UFR de Pharmacie, 1 rue des Louvels 80037 Amiens, France.
authors.forename: François
authors.initials: F
authors.lastname: Mesnard
authors.affiliation: Laboratoire LBLGC EA 1207, Antenne Scientifique Universitaire de Chartres Univ Orleans, 21 rue de Loigny la Bataille, 28000 Chartres, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Lainé
mesh_terms: D002072:Butylene Glycols / Q000737:chemistry / Q000378:metabolism; D019597:Flax / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D005663:Furans / Q000737:chemistry / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism; D017705:Lignans / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D034622:RNA Interference; D012639:Seeds / Q000737:chemistry / Q000201:enzymology / Q000235:genetics
chemical_list: D002072:Butylene Glycols; D005663:Furans; D005960:Glucosides; D017705:Lignans; D010940:Plant Proteins; C051457:dehydrodiconiferyl glucoside; C060282:lariciresinol; D010088:Oxidoreductases; C084968:lariciresinol reductase; C084969:pinoresinol reductase; C060283:secoisolariciresinol; C103298:pinoresinol
keywords: Flax; Lignans; RNA interference; Secoisolariciresinol