title: Effect of enzyme-aided cell wall disintegration on protein extractability from intact and dehulled rapeseed (Brassica rapa L. and Brassica napus L.) press cakes.
abstract: Cell-wall- and pectin-degrading enzyme preparations were used to enhance extractability of proteins from rapeseed press cake. Rapeseed press cakes from cold pressing of intact Brassica rapa and partially dehulled Brassica napus seeds, containing 36-40% protein and 35% carbohydrates, were treated with pectinolytic (Pectinex Ultra SP-L), xylanolytic (Depol 740L), and cellulolytic (Celluclast 1.5L) enzyme preparations. Pectinex caused effective disintegration of embryonic cell walls through hydrolysis of pectic polysaccharides and glucans and increased protein extraction by up to 1.7-fold in comparison to treatment without enzyme addition. Accordingly, 56% and 74% of the total protein in the intact and dehulled press cakes was extracted. Light microscopy of the press cakes suggested the presence of pectins colocalized with proteins inside the embryo cells. Hydrolysis of these intracellular pectins and deconstruction of embryonic cell walls during Pectinex treatment were concluded to relate with enhanced protein release.
authors.affiliation: VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Finland.
authors.forename: Katariina
authors.initials: K
authors.lastname: Rommi
authors.forename: Terhi K
authors.initials: TK
authors.lastname: Hakala
authors.forename: Ulla
authors.initials: U
authors.lastname: Holopainen
authors.forename: Emilia
authors.initials: E
authors.lastname: Nordlund
authors.forename: Kaisa
authors.initials: K
authors.lastname: Poutanen
authors.forename: Raija
authors.initials: R
authors.lastname: Lantto
mesh_terms: D000821:Animal Feed / Q000032:analysis; D029688:Brassica napus / Q000737:chemistry* / Q000166:cytology; D029741:Brassica rapa / Q000737:chemistry* / Q000166:cytology; D002473:Cell Wall / Q000378:metabolism*; D004044:Dietary Proteins / Q000737:chemistry / Q000302:isolation & purification; D005387:Finland; D005511:Food Handling; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008856:Microscopy, Fluorescence; D019441:Plant Epidermis / Q000737:chemistry / Q000166:cytology; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D011134:Polysaccharides / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry* / Q000166:cytology; D012995:Solubility
chemical_list: D004044:Dietary Proteins; D010940:Plant Proteins; D011134:Polysaccharides; D006026:Glycoside Hydrolases