title: Response surface optimization of mucilage aqueous extraction from flixweed (Descurainia sophia) seeds.
abstract: The effects of four factors of pH (4-8), temperature (50-95°C), weed-seed ratio (W/S, 15-45), and time (1-4 h) on the yield of mucilage extraction of Descurainia sophia seeds were investigated using response surface methodology-Box-Behnken design (RSM-BBD). Results showed that a second-order model for the studied response was adequately fitted with a coefficient of determination of 98.7% (p<0.0001). The optimum conditions to achieve the highest yield (10.45%) were extraction time of 2.9 h, extraction temperature of 94.32°C, pH of 7.55 and the W/S ratio of 44.2. The extracted mucilage at the optimal point effectively scavenged DPPH free radical, and more concentrations of this polysaccharide indicated potent antioxidant activity in a dose-dependent manner.
authors.affiliation: Bioprocess and Biodetection Laboratory (BBL), Department of Food Science, Engineering & Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran.
authors.forename: Mahshid
authors.initials: M
authors.lastname: Golalikhani
authors.affiliation: Bioprocess and Biodetection Laboratory (BBL), Department of Food Science, Engineering & Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran. Electronic address: khodaiyan@ut.ac.ir.
authors.forename: Faramarz
authors.initials: F
authors.lastname: Khodaiyan
authors.affiliation: Bioprocess and Biodetection Laboratory (BBL), Department of Food Science, Engineering & Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran.
authors.forename: Azin
authors.initials: A
authors.lastname: Khosravi
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D019607:Brassicaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D010936:Plant Extracts / Q000737:chemistry*; D062087:Plant Mucilage / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D013696:Temperature
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D062087:Plant Mucilage
keywords: Descurainia sophia mucilage; Extraction process; Free radical scavenging activity; Polysaccharide-hydrocolloid; Response surface methodology (RSM)