title: Efficient free fatty acid production from woody biomass hydrolysate using metabolically engineered Escherichia coli.
abstract: Four engineered Escherichia coli strains, ML103(pXZ18), ML103(pXZ18Z), ML190(pXZ18) and ML190(pXZ18Z), were constructed to investigate free fatty acid production using hydrolysate as carbon source. These strains exhibited efficient fatty acid production when xylose was used as the sole carbon source. For mixed sugars, ML103 based strains utilized glucose and xylose sequentially under the carbon catabolite repression (CCR) regulation, while ML190 based strains, with ptsG mutation, used glucose and xylose simultaneously. The total free fatty acid concentration and yield of the strain ML190(pXZ18Z) based on the mixed sugar reached 3.64 g/L and 24.88%, respectively. Furthermore, when hydrolysate from a commercial plant was used as the carbon source, the strain ML190(pXZ18Z) can produce 3.79 g/L FFAs with a high yield of 21.42%.
authors.affiliation: Department of Bioengineering, Rice University, Houston, TX, United States.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Bioengineering, Rice University, Houston, TX, United States.
authors.forename: Jane
authors.initials: J
authors.lastname: Lee
authors.affiliation: Technology Holding, Salt Lake City, UT, United States.
authors.forename: Mukund
authors.initials: M
authors.lastname: Karanjikar
authors.affiliation: Department of Bioengineering, Rice University, Houston, TX, United States; Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, United States. Electronic address: ksan@rice.edu.
authors.forename: Ka-Yiu
authors.initials: KY
authors.lastname: San
mesh_terms: D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis*; D005947:Glucose / Q000494:pharmacology; D006868:Hydrolysis; D060847:Metabolic Engineering / Q000379:methods*; D014934:Wood / Q000737:chemistry*; D014994:Xylose / Q000494:pharmacology
chemical_list: D005230:Fatty Acids, Nonesterified; D002244:Carbon; D014994:Xylose; D005947:Glucose
keywords: Escherichia coli; Fatty acid production; Hydrolysate; Xylose; ptsG