title: Microcirculation density and maturity in uterine and soft tissue leiomyosarcomas: an immunohistochemical study.
abstract: The role of angiogenesis as a hallmark of tumor progression has been poorly explored in leiomyosarcoma, a rare but aggressive mesenchymal malignancy. We aimed to characterize microvessel distribution and morphology - including pericyte coverage - in a retrospective series of leyomiosarcomas of the soft tissues and the uterus. 41 whole-block tumor slides from formalin-fixed paraffin-embedded tissues were immunostained for endothelial-specific marker CD31 and microvessel density was quantified by assigning a grade to the frequency of CD31 positive microvessels. Vessel morphology and pericyte coverage were investigated by double-labeling for CD31 and either PDGFRβ, αSMA, desmin, CD90, or CD146. We found that microvessel density correlated with tumor grade in leiomyosarcoma of soft tissues, in analogy with what has been established in several types of carcinoma. This did not apply to uterine leiomyosarcoma, possibly due to the abundant myometrial vascularization. The evaluation of perivascular cell markers related to vessel stability revealed immature microvascular networks with aberrant pericyte coverage, irrespective of tumor origin or grade. Our observations substantiate the role of angiogenesis in the progression of soft tissue leiomyosarcoma. A multiple-marker approach to the assessment of pericyte coverage can identify different profiles of vessel immaturity correlated with tumor grade.
authors.affiliation: Surgical Pathology Unit, University Hospital of Parma, Parma, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Caraffi
authors.affiliation: Department of Biomedical, Biotechnological and Translational Sciences, University of Parma, Parma, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Corradi
authors.affiliation: Department of Biomedical, Biotechnological and Translational Sciences, University of Parma, Parma, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Campanini
authors.affiliation: Department of Biomedical, Biotechnological and Translational Sciences, University of Parma, Parma, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Govoni
authors.affiliation: Surgical Pathology Unit, University Hospital of Parma, Parma, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Rocchi
authors.affiliation: Centre for Molecular and Translational Oncology (COMT), University of Parma, Parma, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Perris
authors.affiliation: Surgical Pathology Unit, University Hospital of Parma, and Centre for Molecular and Translational Oncology (COMT), University of Parma, Parma, Italy. dmangieri@ao.pr.it.
authors.forename: Domenica
authors.initials: D
authors.lastname: Mangieri
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D006801:Humans; D007890:Leiomyosarcoma / Q000378:metabolism*; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism* / Q000473:pathology; D020286:Pericytes / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D012189:Retrospective Studies; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000473:pathology; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor