title: Simultaneous determination of atropine, scopolamine, and anisodamine from Hyoscyamus niger L. in rat plasma by high-performance liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetics study.
abstract: In order to investigate the pharmacokinetics of tropane alkaloids in Hyoscyamus niger L., a sensitive and specific high-performance liquid chromatography with tandem mass spectrometry method for the simultaneous determination of atropine, scopolamine, and anisodamine in rat plasma is developed and fully validated, using homatropine as an internal standard. The separation of the four compounds was carried out on a BDS Hypersil C18 column using a mobile phase consisting of acetonitrile and water (containing 10 mmol ammonium acetate). Calibration curves were linear from 0.2 to 40 ng/mL for atropine, scopolamine, and from 0.08 to 20 ng/mL for anisodamine. The precision of three analytes was <5.89% and the accuracy was between -1.04 to 2.94%. This method is successfully applied to rat pharmacokinetics analysis of the three tropane alkaloids after oral administration of H. niger extract. The maximum concentration of these three tropane alkaloids was reached within 15 min, and the maximum concentrations were 31.36 ± 7.35 ng/mL for atropine, 49.94 ± 2.67 ng/mL for scopolamine, and 2.83 ± 1.49 ng/mL for anisodamine. The pharmacokinetic parameters revealed areas under the curve of 22.76 ± 5.80, 16.80 ± 3.08, and 4.31 ± 1.21 ng/h mL and mean residence times of 2.08 ± 0.55, 1.19 ± 0.45, and 3.28 ± 0.78 h for atropine, scopolamine, and anisodamine, respectively.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Peiting
authors.initials: P
authors.lastname: Zhang
authors.forename: Yemeng
authors.initials: Y
authors.lastname: Li
authors.forename: Guanghui
authors.initials: G
authors.lastname: Liu
authors.forename: Xiuman
authors.initials: X
authors.lastname: Sun
authors.forename: Yuting
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Deng
authors.forename: Qiongfeng
authors.initials: Q
authors.lastname: Liao
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Xie
mesh_terms: D000818:Animals; D001285:Atropine / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D032304:Hyoscyamus / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012601:Scopolamine / Q000097:blood* / Q000493:pharmacokinetics*; D012991:Solanaceous Alkaloids / Q000097:blood* / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry
chemical_list: D010936:Plant Extracts; D012991:Solanaceous Alkaloids; C003922:anisodamine; D001285:Atropine; D012601:Scopolamine
keywords: Antispasmodic agent; Hyoscyamus niger L; Pharmacokinetics; Tropane alkaloids