title: Cluster of differentiation 24 monoclonal antibody induces apoptosis in the osteosarcoma cells.
abstract: OBJECTIVE
  Cluster of differentiation 24 (CD24) was overexpressed in osteosarcoma and positive CD24 expression correlates significantly with distant metastasis invasion and poor survival in osteosarcomas. We, therefore, suggested that CD24 would be a new molecular target for therapeutic strategies. In the present study, we aimed to investigate the effects of CD24 down-regulation using monoclonal antibodies (mAb) on apoptosis in osteosarcoma cells in vitro and in vivo.
  
  
  METHODS
  Osteosarcoma MG-63 cells were treated with Anti-CD24 mAb, and the effects on growth and apoptosis were evaluated in vitro and in vivo.
  
  
  RESULTS
  Anti-CD24 mAb could induce the apoptosis of cultured MG63 cells and anti-CD24 mAb treatment inhibited the tumor growth after cancer cell grafting and enhanced the cell apoptosis inside the tumor tissue.
  
  
  CONCLUSIONS
  The findings showed that anti-CD24 mAb targeting therapy provides a new avenue toward treating osteosarcoma.
authors.affiliation: Department of Orthopedics, Yishui central hospital, Linyi, Shan Dong Province, China. zhanglinmzs@126.com.
authors.forename: Y-H
authors.initials: YH
authors.lastname: Zhang
authors.forename: Z-Y
authors.initials: ZY
authors.lastname: Wang
authors.forename: F-Y
authors.initials: FY
authors.lastname: Hao
authors.forename: L
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D051927:CD24 Antigen / Q000096:biosynthesis / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D058990:Molecular Targeted Therapy; D012516:Osteosarcoma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D011897:Random Allocation; D023041:Xenograft Model Antitumor Assays
chemical_list: D000911:Antibodies, Monoclonal; D051927:CD24 Antigen; C497889:CD24 protein, human