title: Synthesis and properties of oligonucleotides modified with 2'-O-(2-carboxyethyl)nucleotides and their carbamoyl derivatives.
abstract: 2'-O-Methyl oligoribonucleotides with four kinds of 2'-O-modified uridine derivatives were synthesised. Their duplex stability, hydration behavior and exonuclease resistance were studied by spectroscopic analyses and molecular dynamics simulations. Consequently, 2'-O-modification of the uridine residue with 2-carbamoylethyl or 2-(N-methylcarbamoyl)ethyl groups resulted in a significant improvement of the exonuclease resistance without the loss of duplex stability.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, J2-12, 4259 Nagatsuta-cho, Midoriku, Yokohama, Japan. msekine@bio.titech.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Masaki
authors.forename: Natsuki
authors.initials: N
authors.lastname: Okaniwa
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanamori
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Tsunoda
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D056004:Molecular Dynamics Simulation; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D014529:Uridine / Q000031:analogs & derivatives / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D014529:Uridine