title: Annexin A3 plays a role in cytoplasmic calcium oscillation by extracellular calcium in the human promyelocytic leukemia HL-60 cells differentiated by phorbol-12-myristate-13-acetate.
abstract: The roles of annexin A3 (ANXA3) in macrophages are not fully understood. In contrast to C5a, we have demonstrated that C-terminal ribosomal protein S19 (RP S19)-tagged S-tagged C5a (S-tagged C5a/RP S19) raises an alternative cytoplasmic calcium oscillation by extracellular calcium during macrophage migration into apoptotic cells. We here differentiated human promyelocytic leukemia HL-60 cells bearing with either control sense RNA and shRNA for ANXA3 mRNA or a vector cDNA with or without ANXA3 cDNA into macrophage-like cells by phorbol-12-myristate-13-acetate and found that a fluorescence ratio (340 nm/380 nm) upon the S-tagged C5a/RP S19-induced alternative cytoplasmic calcium oscillation by extracellular calcium was an equilateral association with a dose of ANXA3. Moreover, the ANXA3-dependent modification was partially reflected upon the S-tagged C5a-induced classical cytoplasmic calcium oscillation by both intracellular calcium and extracellular calcium. ANXA3 seems to extend the C5aR-mediated cytoplasmic calcium oscillation by extracellular calcium at least in the HL-60 macrophage-like cells.
authors.affiliation: Division of Functional Pathology, Department of Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan. Electronic address: hi-nishiura@hyo-med.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishiura
authors.affiliation: Division of Functional Pathology, Department of Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yamanegi
authors.affiliation: Department of Dentistry and Oral Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan.
authors.forename: Mutsuki
authors.initials: M
authors.lastname: Kawabe
authors.affiliation: Division of Functional Pathology, Department of Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan.
authors.forename: Nahoko
authors.initials: N
authors.lastname: Kato-Kogoe
authors.affiliation: Division of Functional Pathology, Department of Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Yamada
authors.affiliation: Division of Functional Pathology, Department of Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Nakasho
mesh_terms: D017318:Annexin A3 / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D020013:Calcium Signaling; D002454:Cell Differentiation; D018922:HL-60 Cells; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D044087:Receptor, Anaphylatoxin C5a / Q000235:genetics / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: C488033:C5AR1 protein, human; D044087:Receptor, Anaphylatoxin C5a; D017318:Annexin A3; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium
keywords: Annexin A3; C5a receptor; Calcium; Macrophages; RP S19