title: High-resolution reconstruction of the beating zebrafish heart.
abstract: The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Mickoleit
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schmid
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Weber
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Florian O
authors.initials: FO
authors.lastname: Fahrbach
authors.affiliation: 1] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. [2].
authors.forename: Sonja
authors.initials: S
authors.lastname: Hombach
authors.affiliation: 1] Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany. [2] Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA.
authors.forename: Sven
authors.initials: S
authors.lastname: Reischauer
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Jan
authors.identifier: 0000000172503756
authors.initials: J
authors.lastname: Huisken
mesh_terms: D000465:Algorithms; D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique; D015027:Zebrafish / Q000033:anatomy & histology* / Q000502:physiology