title: Hemoglobin regulates the migration of glioma cells along poly(ε-caprolactone)-aligned nanofibers.
abstract: Aligned fibers have been shown to facilitate cell migration in the direction of fiber alignment while oxygen (O2 )-carrying solutions improve the metabolism of cells in hypoxic culture. Therefore, U251 aggregate migration on poly(ε-caprolactone) (PCL)-aligned fibers was studied in cell culture media supplemented with the O2 storage and transport protein hemoglobin (Hb) obtained from bovine, earthworm and human sources at concentrations ranging from 0 to 5 g/L within a cell culture incubator exposed to O2 tensions ranging from 1 to 19% O2 . Individual cell migration was quantified using a wound healing assay. In addition, U251 cell aggregates were developed and aggregate dispersion/cell migration quantified on PCL-aligned fibers. The results of this work show that the presence of bovine or earthworm Hb improved individual cell viability at 1% O2 , while human Hb adversely affected cell viability at increasing Hb concentrations and decreasing O2 levels. The control data suggests that decreasing the O2 tension in the incubator from 5 to 1% O2 decreased aggregate dispersion on the PCL-aligned fibers. However, the addition of bovine Hb at 5% O2 significantly improved aggregate dispersion. At 19% O2 , Hb did not impact aggregate dispersion. Also at 1% O2 , aggregate dispersion appeared to increase in the presence of earthworm Hb, but only at the latter time points. Taken together, these results show that Hb-based O2 carriers can be utilized to improve O2 availability and the migration of glioma spheroids on nanofibers.
authors.affiliation: William G. Lowrie Dept. of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210.
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Roth
authors.forename: Jacob
authors.initials: J
authors.lastname: Elmer
authors.forename: David R
authors.initials: DR
authors.lastname: Harris
authors.forename: Joseph
authors.initials: J
authors.lastname: Huntley
authors.forename: Andre F
authors.initials: AF
authors.lastname: Palmer
authors.forename: Tyler
authors.initials: T
authors.lastname: Nelson
authors.forename: Jed K
authors.initials: JK
authors.lastname: Johnson
authors.forename: Ruipeng
authors.initials: R
authors.lastname: Xue
authors.forename: John J
authors.initials: JJ
authors.lastname: Lannutti
authors.forename: Mariano S
authors.initials: MS
authors.lastname: Viapiano
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D009835:Oligochaeta; D010108:Oxyhemoglobins / Q000494:pharmacology*; D011091:Polyesters / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D003470:Culture Media; D010108:Oxyhemoglobins; D011091:Polyesters; C016240:polycaprolactone
keywords: glioma cells; hemoglobin