title: [Protection and bidirectional effect of rhubarb anthraquinone and tannins for rats' liver].
abstract: OBJECTIVE
  To compare the bidirectional effect of rhubarb total anthraquinone (TA) and total tannins (TT) on rats' liver.
  
  
  METHODS
  One hundred rats were randomly divided into 10 groups, i.e., the blank group, the model group, the blank + high dose TA group, the blank +low dose TA group, the blank + high dose TT group, the blank + low dose TT group, the model + high dose TA group, the model + low dose TA group, the model +high dose TT group, and the model + low dose TT group, 10 in each group. The carbon tetrachloride (CCI4) was used to prepare the acute liver injury rat model. TA and TT of rhubarb (at 5.40 g crude drugs/kg and 14.69 g crude drugs/kg) were intragastrically administrated to rats in all groups except the blank group and the model group, once daily for 6 successive days.The general state of rats, biochemical indices such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), laminin (LN), hyaluronic acid (HA), transforming growth factor beta1 (TGF-beta1), as well pathological results of rat liver tissues. Finally the protection laws of TA and TT for rats' liver were analyzed using factor analysis.
  
  
  RESULTS
  Compared with the blank control group, all biochemical indices increased in the blank group (P < 0.05, P < 0.01). HA also increased in the blank + high dose TA group; AST, ALT, and HA also increased in the blank +high dose TT group (P < 0.05). Compared with the model group, AST, ALT, ALP, HA, and TGF-beta1 significantly decreased in the model + low dose TA group, the model + high dose TA group, the model + low dose TT group (P < 0.05, P < 0.01). Serum AST, ALT, and ALP also decreased in the model + high dose TT group (P < 0.05, P < 0.01). Pathological results showed that mild swollen liver cells in the model + high dose TA group. Fatty degeneration and fragmental necrosis around the central veins occurred in the blank + high dose TA group. The pathological injury was inproved in the model +low dose TA group. Two common factors, liver fibrosis and liver cell injury, were extracted by using factor analysis. TA showed stronger improvement of the two common factors than TT.
  
  
  CONCLUSIONS
  Rhubarb TA and TT showed protective and harmful effects on rats' liver. At an equivalent dosage, TA had better liver protection than TT. High dose TT played a role in liver injury to some extent.
authors.forename: Lu-shan
authors.initials: LS
authors.lastname: Qin
authors.forename: Hai-ping
authors.initials: HP
authors.lastname: Zhao
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Zhao
authors.forename: Zhi-jiel
authors.initials: ZJ
authors.lastname: Ma
authors.forename: Ling-na
authors.initials: LN
authors.lastname: Zeng
authors.forename: Ya-ming
authors.initials: YM
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Yan
authors.forename: Zhao-fang
authors.initials: ZF
authors.lastname: Bai
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Qing-xiu
authors.initials: QX
authors.lastname: Hao
authors.forename: Kui-jun
authors.initials: KJ
authors.lastname: Zhao
authors.forename: Jia-bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Xiao-he
authors.initials: XH
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000009:adverse effects / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005260:Female; D008099:Liver / Q000187:drug effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012250:Rheum / Q000737:chemistry*; D013634:Tannins / Q000009:adverse effects / Q000494:pharmacology*
chemical_list: D000880:Anthraquinones; D013634:Tannins; D002251:Carbon Tetrachloride