title: Analysis of the linear epitope for Fc-binding on the mouse IgG Fc receptor (moFcγRI) by synthetic peptide.
abstract: To identify the linear epitope for Fc-binding to the mouse immunoglobulin G (IgG) Fc receptor (moFcγRI), peptides derived from the membrane-distal extracellular domain (EC2) of moFcγRI, corresponding to the homologous region of human FcγRI (huFcγRI) and huFcγRII, were synthesized. Using a dot-blot assay, six peptides were tested. The results showed that the moRI3 peptide (CVFYRNGKSFQFS) could combine with mouse IgG efficiently. A competitive enzyme-linked immunosorbent assay (ELISA) showed that the IC50 value of the moRI3 peptide was 38.03 mM. The moRI3 peptide could inhibit the combination of mouse IgG to the transfected COS 7 cells significantly with an IC50 value of 72.68 mM. The IgG-binding region of moFcγRI was also localized in the C'-E loop of the EC2 domain as predicted according to huFcγRI and huFcγRII. We predicted that the minimum effective IgG-binding region of moFcγRI may be the peptide 153SFQFSS158. The linear epitope for immunoglobulin-binding to mouse FcγR is also described. Thus, we generated a peptide that targets a fundamental aspect of ligand recognition by this receptor class.
authors.affiliation: Henan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China.
authors.forename: F Y
authors.initials: FY
authors.lastname: Wang
authors.affiliation: Henan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Guo
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China sprinkle.w@126.com.
authors.forename: G P
authors.initials: GP
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D051379:Mice; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000276:immunology / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism*
chemical_list: D000939:Epitopes; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D010446:Peptide Fragments; D017452:Receptors, IgG