title: A role for exocyst in maturation and bactericidal function of staphylococci-containing endothelial cell phagosomes.
abstract: Bacteria that invade human endothelial cells can be efficiently eliminated in phagolysosomes. We investigated the role of vesicle tethering exocyst complex in maturation and function of endothelial cell phagosomes harbouring staphylococci or latex beads. Exocyst complex proteins (Sec5, -8, -10, Exo70) together with recycling endosome marker Rab11 were detected in vesicles that dynamically interacted and seemingly fused with endothelial cell phagosomes. Knockdown of exocyst proteins Sec8 and Exo70 inhibited the accumulation of Rab11-positive vesicles at the phagosomes. Furthermore, knockdown of exocyst proteins and Rab11 greatly reduced acidification of phagosomes and significantly diminished the elimination of invaded staphylococci in endothelial cells. The inhibitory effect of Exo70 knockdown on bacterial elimination could be rescued by constitutively active Rab11-Q70L. Our data suggest that exocyst complex controls the interaction of recycling endocytic vesicles with phagosomes and this process is involved in maturation and functioning of the phagosomes in endothelial cells.
authors.affiliation: Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, D-20246, Hamburg, Germany.
authors.forename: Liane
authors.initials: L
authors.lastname: Rauch
authors.forename: Kirsten
authors.initials: K
authors.lastname: Hennings
authors.forename: Martin
authors.initials: M
authors.lastname: Aepfelbacher
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism*; D002478:Cells, Cultured; D004705:Endocytosis; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008863:Microspheres; D010588:Phagosomes / Q000378:metabolism*; D013210:Staphylococcus / Q000737:chemistry; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D000099193:rab11 GTP-Binding Proteins
chemical_list: D018829:Adhesins, Bacterial; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C489607:EXOC4 protein, human; C547754:EXOC7 protein, human; C078635:fibronectin-binding proteins, bacterial
keywords: endothelium; exocyst; phagosome maturation; recycling endosome; staphylococci