title: Binding between lead ions and the high-abundance serum proteins.
abstract: The interaction between three of the most abundant bovine serum proteins (serum albumin, transferrin and IgG) with Pb(2+) was investigated using electrochemistry. The data was used to construct a new theoretical model of Pb(2+) binding to the high-abundance serum proteins under non-ideal conditions. The binding constants (β) of Pb(2+) to the individual proteins and a mixture of proteins were measured according to a new theoretical equation (non-ideal state) as well as the McGhee-Von Hippel equation (ideal state). Differences between the models suggested that the β values obtained using the non-ideal state model was more realistic. Protein-protein interactions and micro-environmental influences affected binding between Pb(2+) and the high-abundance serum proteins. We included a micro-environmental influence factor for the model (Fm), which accurately quantified the effect of micro-environment of the proteome of Pb(2+) binding with the serum proteins. This research provides a useful reference of theoretical and experimental work regarding heavy-metal binding interactions with serum proteins.
authors.affiliation: School of Science, Zhejiang Agricultural & Forestry University, Lin'an, Zhejiang Province 311300, China. Electronic address: tdky@hotmail.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Guo
authors.affiliation: School of Science, Zhejiang Agricultural & Forestry University, Lin'an, Zhejiang Province 311300, China.
authors.forename: Ling
authors.initials: L
authors.lastname: He
authors.affiliation: Centre for Solid Waste Bioprocessing, School of Civil Engineering and School of Chemical Engineering, The University of Queensland, Brisbane St Lucia, QLD 4072, Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: Strong
authors.affiliation: School of Environmental and Resource Sciences, Zhejiang Agricultural & Forestry University, Lin'an, Zhejiang Province 311300, China. Electronic address: nzhailongwang@gmail.com.
authors.forename: Hailong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002417:Cattle; D004563:Electrochemistry; D004785:Environmental Pollutants / Q000378:metabolism*; D007854:Lead / Q000378:metabolism*; D008956:Models, Chemical; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D004785:Environmental Pollutants; D012709:Serum Albumin; D007854:Lead
keywords: BSA; Binding interaction; Capillary zone electrophoresis; Electrochemical method; Lead ion; Serum proteome