title: Comparative proteomic analysis of amaranth mesophyll and bundle sheath chloroplasts and their adaptation to salt stress.
abstract: The effect of salt stress was analyzed in chloroplasts of Amaranthus cruentus var. Amaranteca, a plant NAD-malic enzyme (NAD-ME) type. Morphology of chloroplasts from bundle sheath (BSC) and mesophyll (MC) was observed by transmission electron microscopy (TEM). BSC and MC from control plants showed similar morphology, however under stress, changes in BSC were observed. The presence of ribulose bisphosphate carboxylase/oxygenase (RuBisCO) was confirmed by immunohistochemical staining in both types of chloroplasts. Proteomic profiles of thylakoid protein complexes from BSC and MC, and their changes induced by salt stress were analyzed by blue-native polyacrylamide gel electrophoresis followed by SDS-PAGE (2-D BN/SDS-PAGE). Differentially accumulated protein spots were analyzed by LC-MS/MS. Although A. cruentus photosynthetic tissue showed the Kranz anatomy, the thylakoid proteins showed some differences at photosystem structure level. Our results suggest that A. cruentus var. Amaranteca could be better classified as a C3-C4 photosynthetic plant.
authors.affiliation: IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Lomas 4a Sección, San Luis Potosí, S.L.P. 78216, Mexico.
authors.forename: Ahuitzolt
authors.initials: A
authors.lastname: Joaquín-Ramos
authors.affiliation: IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Lomas 4a Sección, San Luis Potosí, S.L.P. 78216, Mexico.
authors.forename: José Á
authors.initials: JÁ
authors.lastname: Huerta-Ocampo
authors.affiliation: IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Lomas 4a Sección, San Luis Potosí, S.L.P. 78216, Mexico.
authors.forename: Alberto
authors.initials: A
authors.lastname: Barrera-Pacheco
authors.affiliation: IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Lomas 4a Sección, San Luis Potosí, S.L.P. 78216, Mexico.
authors.forename: Antonio
authors.initials: A
authors.lastname: De León-Rodríguez
authors.affiliation: Martin-Luther-Universität Halle-Wittenberg, Institut für Biochemie, Abteilung Pflanzenbiochemie, Weinbergweg 22 (Biozentrum), 06120 Halle (Saale), Germany.
authors.forename: Sacha
authors.initials: S
authors.lastname: Baginsky
authors.affiliation: IPICyT, Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Lomas 4a Sección, San Luis Potosí, S.L.P. 78216, Mexico. Electronic address: apbarba@ipicyt.edu.mx.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Barba de la Rosa
mesh_terms: D000222:Adaptation, Physiological; D027721:Amaranthus / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D002853:Chromatography, Liquid; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel; D045342:Light-Harvesting Protein Complexes; D058503:Mesophyll Cells; D046529:Microscopy, Electron, Transmission; D046912:Multiprotein Complexes; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D058526:Plant Vascular Bundle / Q000378:metabolism; D040901:Proteomics; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry; D020524:Thylakoids / Q000378:metabolism
chemical_list: D045342:Light-Harvesting Protein Complexes; D046912:Multiprotein Complexes; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D012965:Sodium Chloride
keywords: BN/SDS-PAGE; Bundle sheath; Chloroplasts; LC/MS–MS; Mesophyll