title: Phylogenetic relationships of 3 Korean Neodiplostomum species (Digenea: Neodiplostomidae) based on partial CO1 gene.
abstract: The phylogenetic relationships of the 3 Neodiplostomum spp. (Digenea: Neodiplostomidae) occurring in Korea (N. seoulense, N. leei, and N. boryongense) were analyzed using the partial mitochondrial cytochrome c oxidase subunit 1 (CO1) gene. The adult flukes were recovered from Sprague-Dawley rats (N. seoulense) and newborn chicks (N. leei and N. boryongense) experimentally infected with the neodiplostomula from the grass snake, Rhabdophis tigrinus tigrinus. The genomic DNA was amplified using specific primers, and the sequence of CO1 was obtained. According to the results, the pairwise similarity was 96.1% between N. boryongense and N. seoulense, but was 95.0% between N. boryongense and N. leei and 94.2% between N. leei and N. seoulense. The results demonstrated a closer phylogenetic relationship between N. seoulense and N. boryongense. This high relationship of N. seoulense and N. boryongense may be related to their similar morphologic features including the limited distribution of vitellaria and the presence of a genital cone. N. leei is distinct on the other hand with an extensive distribution of vitellaria and the absence of a genital cone.
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Kyoung-Ho
authors.initials: KH
authors.lastname: Pyo
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Jo Woon
authors.initials: JW
authors.lastname: Yi Lee
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: Department of Parasitology, Inha University College of Medicine, Incheon 400-712, Korea.
authors.forename: Yun-Kyu
authors.initials: YK
authors.lastname: Park
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea. ; Seoul National University Bundang Hospital, Seongnam 463-707, Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Shin
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D016000:Cluster Analysis; D017812:Colubridae / Q000469:parasitology*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014200:Trematoda / Q000145:classification* / Q000235:genetics*
chemical_list: D018509:DNA, Helminth; D003576:Electron Transport Complex IV
keywords: CO1 gene; Neodiplostomum boryongense; Neodiplostomum leei; Neodiplostomum seoulense; phylogeny