title: Interactions between anticancer trans-platinum compounds and proteins: crystal structures and ESI-MS spectra of two protein adducts of trans-(dimethylamino)(methylamino)dichloridoplatinum(II).
abstract: The adducts formed between trans-(dimethylamino)(methylamino)dichloridoplatinum(II), [t-PtCl2(dma)(ma)], and two model proteins, i.e., hen egg white lysozyme and bovine pancreatic ribonuclease, were independently characterized by X-ray crystallography and electrospray ionization mass spectrometry. In these adducts, the Pt(II) center, upon chloride release, coordinates either to histidine or aspartic acid residues while both alkylamino ligands remain bound to the metal. Comparison with the cisplatin derivatives of the same proteins highlights for [t-PtCl2(dma)(ma)] a kind of biomolecular metalation remarkably different from that of cisplatin.
authors.affiliation: Department of Chemistry, University of Florence , Via della Lastruccia 3, 50019, Sesto Fiorentino, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Messori
authors.forename: Tiziano
authors.initials: T
authors.lastname: Marzo
authors.forename: Elena
authors.initials: E
authors.lastname: Michelucci
authors.forename: Irene
authors.initials: I
authors.lastname: Russo Krauss
authors.forename: Carmen
authors.initials: C
authors.lastname: Navarro-Ranninger
authors.forename: Adoracion G
authors.initials: AG
authors.lastname: Quiroga
authors.forename: Antonello
authors.initials: A
authors.lastname: Merlino
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D002417:Cattle; D018360:Crystallography, X-Ray; D009113:Muramidase / Q000737:chemistry; D017671:Platinum Compounds / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000970:Antineoplastic Agents; D017671:Platinum Compounds; D011506:Proteins; D012259:Ribonuclease, Pancreatic; C421864:hen egg lysozyme; D009113:Muramidase