title: Ascorbic acid enhances oxidative stress tolerance and biological control efficacy of Pichia caribbica against postharvest blue mold decay of apples.
abstract: The effect of ascorbic acid (VC) on improving oxidative stress tolerance of Pichia caribbica and biocontrol efficacy against blue mold caused by Penicillium expansum on apples was investigated. P. caribbica showed susceptibility to the oxidative stress in vitro test, and 250 μg/mL VC treatment improved its oxidative stress tolerance. The higher viability exhibited by VC-treated yeast was associated with a lower intracellular ROS level. The activities of antioxidant enzymes of P. caribbica were improved by VC treatment, including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX). Additionally, VC-treated yeast exhibited greater biocontrol activity against P. expansum and faster growth when stored at 25 and 4 °C, respectively, compared to the performance of the non-VC-treated yeast. In response to the VC treatment under oxidative stress, several differentially expressed proteins were identified in P. caribbica, and most of the poteins were confirmed to be related to basic metabolism. Therefore, the application of ascorbic acid is a useful approach to improve oxidative stress tolerance of P. caribbica and its biocontrol efficacy on apples.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University , Zhenjiang 212013, Jiangsu, People's Republic of China.
authors.forename: Chaolan
authors.initials: C
authors.lastname: Li
authors.forename: Hongyin
authors.initials: H
authors.lastname: Zhang
authors.forename: Qiya
authors.initials: Q
authors.lastname: Yang
authors.forename: Mahunu Gustav
authors.initials: MG
authors.lastname: Komla
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhang
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhu
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D061046:Biological Control Agents; D002374:Catalase / Q000378:metabolism; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods; D005638:Fruit / Q000378:metabolism / Q000382:microbiology; D015966:Gene Expression Regulation, Fungal; D005979:Glutathione Peroxidase / Q000378:metabolism; D027845:Malus / Q000187:drug effects / Q000382:microbiology*; D018384:Oxidative Stress / Q000187:drug effects*; D010407:Penicillium / Q000187:drug effects* / Q000254:growth & development; D010843:Pichia / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D017382:Reactive Oxygen Species; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013482:Superoxide Dismutase / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D061046:Biological Control Agents; D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid