title: Traditional chinese medicine shuang shen ning xin attenuates myocardial ischemia/reperfusion injury by preserving of mitochondrial function.
abstract: To investigate the potential cardioprotective effects of Shuang Shen Ning Xin on myocardial ischemia/reperfusion injury. Wistar rats were treated with trimetazidine (10 mg/kg/day, ig), Shuang Shen Ning Xin (22.5, 45 mg/kg/day, ig), or saline for 5 consecutive days. Myocardial ischemia/reperfusion injury was induced by ligation of the left anterior descending coronary artery for 40 min and reperfusion for 120 min on the last day of administration. It is found that Shuang Shen Ning Xin pretreatment markedly decreased infarct size and serum LDH levels, and this observed protection was associated with reduced myocardial oxidative stress and cardiomyocyte apoptosis after myocardial ischemia/reperfusion injury. In addition, further studies on mitochondrial function showed that rats treated with Shuang Shen Ning Xin displayed decreased mitochondrial swelling and cytosolic cytochrome c levels, which were accompanied by a preservation of complex I activities and inhibition of mitochondrial permeability transition. In conclusion, the mitochondrial protective effect of Shuang Shen Ning Xin could be a new mechanism, by which Shuang Shen Ning Xin attenuates myocardial ischemia/reperfusion injury.
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China ; Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1 Xiyuan Caochang, Haidian District, Beijing 100091, China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1 Xiyuan Caochang, Haidian District, Beijing 100091, China.
authors.forename: Jianxun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1 Xiyuan Caochang, Haidian District, Beijing 100091, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1 Xiyuan Caochang, Haidian District, Beijing 100091, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1 Xiyuan Caochang, Haidian District, Beijing 100091, China.
authors.forename: Chengren
authors.initials: C
authors.lastname: Lin
authors.affiliation: Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1 Xiyuan Caochang, Haidian District, Beijing 100091, China.
authors.forename: Cuixiang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, No. 1 Xiyuan Caochang, Haidian District, Beijing 100091, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang