title: Establishment of highly metastatic cell line (Lu10) from murine mammary carcinoma cell line MCH66 and biological characteristics of Lu10.
abstract: OBJECTIVE
  Mouse mammary cancer cell line MCH66 shows invasion-independent metastasis. To elucidate this metastatic mechanism, the biological characteristics putatively related to metastasis were analyzed using several cell lines with different metastatic abilities derived from MCH66.
  
  
  METHODS
  Metastatic capacity, invasive activity, growth property, and mRNA expressions of factors associated with endothelial cell proliferation were comparatively analyzed in MCH66 and its sublines.
  
  
  RESULTS
  Lu10 subline exhibited higher metastatic potential to the lungs and lymph nodes (100%) than MCH66 or Lu1 subline (0/5, 0/5 each). The growth rate was almost identical between Lu10 and MCH66, and Lu10 revealed weaker invasive activity in vitro than MCH66. In Lu10 tumors in mice, well-developed sinusoidal blood vessels and dilated lymphatics were noted compared with in Lu1 tumors. Accordingly, Lu10 showed higher expression of vascular endothelial growth factor (VEGF)-C, -D, platelet-derived growth factor (PDGF)-B and pleiotrophin than Lu1, while the expression of other growth factors such as VEGF-A, midkine, angiogenin, hepatocyte growth factor, PDGF-A, and basic fibroblast growth factor remained unchanged between Lu1 and Lu10.
  
  
  CONCLUSIONS
  These data indicate that high invasiveness and rapid growth are not required for this metastatic process, and some angiogenic mediators are involved in blood-borne and lymphatic metastasis.
authors.affiliation: Department of Pathology, Tokai University school of medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan. g-ogura@tokai-u.jp.
authors.forename: Go
authors.initials: G
authors.lastname: Ogura
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugino
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Naoya
authors.initials: N
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D016207:Cytokines / Q000378:metabolism; D008207:Lymphatic Metastasis; D015674:Mammary Neoplasms, Animal / Q000098:blood supply / Q000235:genetics* / Q000473:pathology*; D008809:Mice, Inbred C3H; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D016207:Cytokines; D020574:Proto-Oncogene Proteins c-sis; D042582:Vascular Endothelial Growth Factor C; C066845:pleiotrophin