title: New carbohydrate-active enzymes identified by screening two metagenomic libraries derived from the soil of a winter wheat field.
abstract: OBJECTIVE
  Soils are rich, diversified environments where β-glucosidases abound because of their importance in organic matter degradation. The aim of this work was to discover new β-glucosidases by constructing two metagenomic DNA libraries from soil samples collected in winter and spring from a field of winter wheat.
  
  
  RESULTS
  Both libraries were screened on esculin-supplemented medium so as to isolate candidates showing β-glucosidase activity. Candidate analysis revealed seven putative β-glycosidases and two putative glycosyltransferases, displaying 25 to 82% identity to known enzymes. The putative β-glycosidases belong to families GH1, GH3 and GH20 and the two putative glycosyltransferases, probably, to new families. In characterization tests performed on bacteria in suspension or spread on agar plates, some candidates appeared to hydrolyse several natural and synthetic substrates. These tests also highlighted interesting industrial characteristics, such as the activity of four β-glycosidases under alkaline conditions and the esculin-hydrolysing activity of a β-glucosidase candidate in the presence of glucose.
  
  
  CONCLUSIONS
  Seven putative β-glycosidases and two putative glycosyltransferases were found by functional screening of two metagenomic DNA libraries derived from agricultural soil.
  
  
  CONCLUSIONS
  This study has identified β-glycosidases and putative glycosyltransferases that have or may have interesting industrial characteristics.
authors.affiliation: Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Stroobants
authors.forename: D
authors.initials: D
authors.lastname: Portetelle
authors.forename: M
authors.initials: M
authors.lastname: Vandenbol
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000235:genetics; D001530:Belgium; D015723:Gene Library; D016695:Glycosyltransferases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007218:Industrial Microbiology; D056186:Metagenomics; D012988:Soil Microbiology; D014908:Triticum; D001617:beta-Glucosidase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D016695:Glycosyltransferases; D001617:beta-Glucosidase
keywords: carbohydrate esterase; functional screening; glucosidase; glycoside hydrolase; glycosyltransferase; hydrolysing enzyme; metagenomic library; soil