title: Protective role of probiotic lactic acid bacteria against dietary fumonisin B1-induced toxicity and DNA-fragmentation in sprague-dawley rats.
abstract: The genus Fusarium, especially F. verticillioides and F. proliferatum, has been found in several agricultural products worldwide, especially in maize. Regardless the occurrence of symptoms, the presence of Fusarium in maize constitutes an imminent risk due to its ability to produce fumonisins, mycotoxins with proven carcinogenic effect on rats, swine, and equines and already classified as possible carcinogens to humans. The toxicity of incremental levels of fumonisin B1 (FB1), that is, 50, 100, and 200 mg FB1/kg diet, and the role of Lactobacillus delbrueckii subsp. lactis DSM 20076 (LL) and Pediococcus acidilactici NNRL B-5627 (PA) supplementation in counteracting the FB1 effects in intoxicated rats were monitored over a period of 4 weeks. Effects on the feed intake and body weight gain were noticed. A significant (p ≤ 0.05) increase in the level of liver and kidney functions markers and DNA fragmentation was also noticed in rat groups T100 and T200. The lactic acid bacteria (LAB) supplementation could bring back the normal serum biochemical parameters in rats fed on fumonisin B1-contaminated diets (T50 and T100) compared to FB1-treated groups. In rats of high-dosage dietary groups supplemented with LAB (T200-LL and T200-PA), the supplementation reduced the serum activity levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and creatinine by 11.3, 11.9, 32, and 20%, respectively. DNA fragmentations were observed in the rat group treated with 200 mg FB1 after 3 weeks, while fragmentation was noticed in treated groups with 100 and 200 mg FB1 after 4 weeks. No DNA fragmentation was apparent in FB1-treated rats co-administered the LL or PA strain. These results suggest that in male rats consuming diets containing FB1, there is a time- and dose-dependent increase in serum enzyme activities and DNA lesions. Moreover, Lb. delbrueckii subsp. lactis (LL) and P. acidilactici (PA) strains have a protective effect against antigenotoxicity and precancerous lesions.
authors.affiliation: a Department of Protein Technology , Institute of Genetic Engineering and Biotechnology, City of Scientific Research and Technological Applications , Bourg Elarab , Alexandria , Egypt.
authors.forename: Ashraf A
authors.initials: AA
authors.lastname: Khalil
authors.forename: Ashgan E
authors.initials: AE
authors.lastname: Abou-Gabal
authors.forename: Amira A
authors.initials: AA
authors.lastname: Abdellatef
authors.forename: Ahmed E
authors.initials: AE
authors.lastname: Khalid
mesh_terms: D000284:Administration, Oral; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000235:genetics / Q000382:microbiology* / Q000517:prevention & control*; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics*; D053938:DNA Fragmentation / Q000187:drug effects*; D005506:Food Contamination; D037341:Fumonisins / Q000008:administration & dosage / Q000633:toxicity*; D007778:Lactobacillus / Q000502:physiology*; D008297:Male; D019936:Probiotics / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013723:Teratogens / Q000633:toxicity; D016896:Treatment Outcome
chemical_list: D037341:Fumonisins; D013723:Teratogens; C056933:fumonisin B1
keywords: DNA fragmentation; Fusarium; fumonisin B1; kidney function; lactic acid bacteria; liver function; toxicity