title: Beneficial effects of houttuynin on ventricular remodeling induced by coronary artery ligation in rats.
abstract: To examine the effects of houttuynin on ventricular remodeling induced by coronary artery ligation in rats and the underlying mechanisms. A rat model of ventricular remodeling was established by left coronary artery ligation (CAL). Rats were randomly divided into four groups: CAL control, CAL plus 40 mg/kg captopril, CAL plus 100 mg/kg houttuynin and sham-operated control. The rats were administered intragastrically with the corresponding drugs or distilled water for 7 weeks. At the end of the experiment, the left ventricular weight index (LVWI) and heart weight index (HWI) were determined. Myocardium tissue was stained with hematoxylin and eosin or picric acid/Sirius red for cardiomyocyte cross-section area or collagen content measurements respectively. The concentrations of angiotensin I (Ang I), angiotensin II (Ang II), aldosterone (ALD) and endothelin-1 (ET-1) in myocardium or serum were detected by radioimmunoassay. The hydroxyproline (Hyp) concentration was measured by alkali hydrolysis. Ultraviolet spectrophotometry was used to determine glutathione peroxidase (GSH-Px) and catalase (CAT) activities in serum. Houttuynin significantly diminished LVWI and HWI, decreased Ang I, Ang II, ALD, ET-1 and Hyp concentrations in myocardium or serum, increased NO concentration and GSH-Px, CAT activities after 7 weeks of treatment. Houttuynin could also reduce cardiomyocyte cross-section area and collagen deposition. Houttuynin attenuates ventricular remodeling in coronary artery ligation rats by restricting the excessive activation of rennin-angiotensin-aldosterone system (RAAS) and the peroxidation.
authors.affiliation: Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, NO. 1200 Cailun Road, Shanghai 201203, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, NO. 1200 Cailun Road, Shanghai 201203, China. Electronic address: zydgjp@hotmail.com.
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Gao
authors.affiliation: Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, NO. 1200 Cailun Road, Shanghai 201203, China.
authors.forename: Chang Xun
authors.initials: CX
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, NO. 1200 Cailun Road, Shanghai 201203, China.
authors.forename: Hui Lin
authors.initials: HL
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, NO. 1200 Cailun Road, Shanghai 201203, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, NO. 1200 Cailun Road, Shanghai 201203, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wu
mesh_terms: D000447:Aldehydes / Q000494:pharmacology*; D000450:Aldosterone / Q000097:blood / Q000378:metabolism; D000803:Angiotensin I / Q000097:blood / Q000378:metabolism; D000804:Angiotensin II / Q000097:blood / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000097:blood; D003094:Collagen / Q000378:metabolism; D003331:Coronary Vessels / Q000601:surgery; D019332:Endothelin-1 / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood; D008026:Ligation; D008297:Male; D009206:Myocardium / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020257:Ventricular Remodeling / Q000187:drug effects*
chemical_list: D000447:Aldehydes; D019332:Endothelin-1; D000804:Angiotensin II; D000450:Aldosterone; C023969:decanoyl acetaldehyde; D003094:Collagen; D000803:Angiotensin I; D002374:Catalase; D005979:Glutathione Peroxidase
keywords: Coronary artery ligation; Houttuynin; Myocardial ischemia; Peroxidase; RAAS; Ventricular remodeling