title: Structural peculiarities of polysaccharide from sterile form of Far Eastern red alga Ahnfeltiopsis flabelliformis.
abstract: KCl-insoluble sulfated polysaccharide from sterile alga Ahnfeltiopsis flabelliformis was investigated. Partial reductive hydrolysis and NMR spectroscopy showed that the polysaccharide comprises disaccharide units of carrabiose only. According to FT-IR-, 1D, 2D NMR spectroscopies and mass-spectrometry this polysaccharide is kappa/beta-carrageenan with ratio of kappa- and beta-types units 3:1 and contains minor amounts of iota- and gamma-carrageenans (precursor of beta-carrageenan). In addition, ESIMS/MS data suggested that xylose (minor amount) is present in the polysaccharide as a substituent one of hydroxyl group of galactose. According to aPTT and PT assays the studied carrageenan affected mostly intrinsic pathway of coagulation, while it effect on the extrinsic pathway is absent.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation. Electronic address: kravchenko_25.89@mail.ru.
authors.forename: Anna O
authors.initials: AO
authors.lastname: Kravchenko
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Stanislav D
authors.initials: SD
authors.lastname: Anastyuk
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Isakov
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Sokolova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Valery P
authors.initials: VP
authors.lastname: Glazunov
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russian Federation.
authors.forename: Irina M
authors.initials: IM
authors.lastname: Yermak
mesh_terms: D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D002351:Carrageenan / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D000461:Rhodophyta / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000925:Anticoagulants; D002351:Carrageenan
keywords: Ahnfeltiopsis flabelliformis; Carrageenan; Mass spectrometry; NMR spectroscopy; Structure