title: Stromal cell-derived factor 1 gene polymorphism is associated with susceptibility to adverse long-term allograft outcomes in non-diabetic kidney transplant recipients.
abstract: Although the genetic polymorphism of Stromal Cell-Derived Factor 1 (SDF-1) is associated with higher mortality of liver allograft recipients, the role of SDF-1 in the modulation of renal allograft outcomes is unclear. Between March 2000 and January 2008, we recruited 252 non-diabetic renal transplant recipients (RTRs). Baseline characteristics and blood chemistry were recorded. Genomic DNA extraction with polymerase chain reaction-restriction fragment length polymorphism was utilized to analyze the genetic polymorphisms of SDF-1 (rs1801157). The influence of SDF-1 on an adverse renal allograft outcome, defined as either a doubling of serum creatinine, graft failure, or patient death was evaluated. Sixteen patients with the SDF-1 AA/AG genotype and nine with the SDF-1 GG genotype reached an adverse outcome. According to Kaplan-Meier analysis, patients carrying the SDF-1 AA/AG genotype or A allele showed a significantly higher risk of reaching an adverse outcome than those carrying the SDF-1 GG genotype or G allele (p=0.041; p=0.0051, respectively; log rank test). Stepwise multivariate Cox proportional regression analysis revealed that patients carrying the SDF-1 AA/AG genotype and A allele had a 2.742-fold (95% CI. 1.106-6.799, p=0.03) and 2.306-fold (95% CI. 1.254-4.24, p=0.008) risk of experiencing an adverse outcome. The SDF-1 AA/AG genotype and A allele have a detrimental impact on the long-term outcome of RTRs.
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan. chung-jieh@yahoo.com.tw.
authors.forename: Chung-Jieh
authors.initials: CJ
authors.lastname: Wang
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan. abi0918@yahoo.com.tw.
authors.forename: Jen-Pi
authors.initials: JP
authors.lastname: Tsai
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan. ysf@csmu.edu.tw.
authors.forename: Shun-Fa
authors.initials: SF
authors.lastname: Yang
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung 402, Taiwan. cshy525@csh.org.tw.
authors.forename: Jong-Da
authors.initials: JD
authors.lastname: Lian
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan. chr@csmu.edu.tw.
authors.forename: Horng-Rong
authors.initials: HR
authors.lastname: Chang
mesh_terms: D000328:Adult; D000483:Alleles; D054377:Chemokine CXCL12 / Q000235:genetics*; D003404:Creatinine / Q000097:blood; D005260:Female; D005838:Genotype; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D023001:Transplantation Tolerance / Q000235:genetics*
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D003404:Creatinine