title: Excision efficiency is not strongly coupled to transgenic rate: cell type-dependent transposition efficiency of sleeping beauty and piggyBac DNA transposons.
abstract: The Sleeping Beauty (SB) and piggyBac (PB) DNA transposons represent an emerging new gene delivery technology, potentially suitable for human gene therapy applications. Previous studies pointed to important differences between these transposon systems, depending on the cell types examined and the methodologies applied. However, efficiencies cannot always be compared because of differences in applications. In addition, "overproduction inhibition," a phenomenon believed to be a characteristic of DNA transposons, can remarkably reduce the overall transgenic rate, emphasizing the importance of transposase dose applied. Therefore, because of lack of comprehensive analysis, researchers are forced to optimize the technology for their own "in-house" platforms. In this study, we investigated the transposition of several SB (SB11, SB32, SB100X) and PB (mPB and hyPB) variants in various cell types at three levels: comparing the excision efficiency of the reaction by real-time PCR, testing the overall transgenic rate by detecting cells with stable integrations, and determining the average copy number when using different transposon systems and conditions. We concluded that high excision activity is not always followed by a higher transgenic rate, as exemplified by the hyperactive transposases, indicating that the excision and the integration steps of transposition are not strongly coupled as previously thought. In general, all levels of transposition show remarkable differences depending on the transposase used and cell lines examined, being the least efficient in human embryonic stem cells (hESCs). In spite of the comparably low activity in those special cell types, the hyperactive SB100X and hyPB systems could be used in hESCs with similar transgenic efficiency and with reasonably low (2-3) transgene copy numbers, indicating their potential applicability for gene therapy purposes in the future.
authors.affiliation: 1 Institute of Enzymology, Research Center for Natural Sciences , Hungarian Academy of Sciences, 1117 Budapest, Hungary .
authors.forename: Orsolya
authors.initials: O
authors.lastname: Kolacsek
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Erdei
authors.forename: Agota
authors.initials: A
authors.lastname: Apáti
authors.forename: Sára
authors.initials: S
authors.lastname: Sándor
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Izsvák
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Ivics
authors.forename: Balázs
authors.initials: B
authors.lastname: Sarkadi
authors.forename: Tamás I
authors.initials: TI
authors.lastname: Orbán
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D018628:Gene Dosage; D005822:Genetic Vectors / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D014162:Transfection; D019076:Transgenes / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements