title: Maspin expression patterns differ in the invasive versus lepidic growth pattern of pulmonary adenocarcinoma.
abstract: OBJECTIVE
  To test whether changes in the subcellular localization of maspin parallel morphological progression in pulmonary adenocarcinoma, we compared its expression between lepidic and invasive growth patterns.
  
  
  METHODS
  Applying immunohistochemistry, we compared maspin expression in lepidic and invasive growth patterns occurring in different tumours (series #1, n = 86) as well as within the same tumour and in the same section (series #2, n = 29).
  
  
  RESULTS
  In both series, the lepidic growth pattern (n = 45) was significantly associated with nuclear maspin, while the invasive (n = 70) with combined nuclear and cytoplasmic maspin (P < 0.05). In the second series, transition from a lepidic to an invasive pattern in the same tumour was associated predominantly with a shift respectively from a nuclear to a combined nuclear and cytoplasmic maspin (15/29) or preservation of nuclear expression (8/29). A shift from nuclear maspin to negative expression (3/29) or other patterns (3/29) were also observed.
  
  
  CONCLUSIONS
  Nuclear maspin is a typical but not exclusive feature of the lepidic growth pattern of pulmonary adenocarcinoma, whereas combined nuclear and cytoplasmic maspin characterizes invasion. These data show that changes of expression and subcellular localization of maspin may constitute an important biological end point of tumour progression and aid in the classification of lung adenocarcinoma.
authors.affiliation: Department of Pathology, School of Medicine, Karmanos Cancer Institute, Wayne State University, Detroit, MI, USA; Tumour Biology and Microenvironment Program, Karmanos Cancer Institute, Wayne State University, Detroit, MI, USA.
authors.forename: Fulvio
authors.initials: F
authors.lastname: Lonardo
authors.forename: Hui
authors.initials: H
authors.lastname: Guan
authors.forename: Sijana
authors.initials: S
authors.lastname: Dzinic
authors.forename: Shijie
authors.initials: S
authors.lastname: Sheng
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D018450:Disease Progression; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D015843:Serpins / Q000096:biosynthesis*; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; C085344:SERPIN-B5; D015843:Serpins
keywords: invasive adenocarcinoma; lepidic growth pattern; maspin; pulmonary adenocarcinoma progression; subcellular localization