title: Short chain N-acyl homoserine lactone production in tropical marine Vibrio sinaloensis strain T47.
abstract: Quorum sensing (QS), acts as one of the gene regulatory systems that allow bacteria to regulate their physiological activities by sensing the population density with synchronization of the signaling molecules that they produce. Here, we report a marine isolate, namely strain T47, and its unique AHL profile. Strain T47 was identified using 16S rRNA sequence analysis confirming that it is a member of Vibrio closely clustered to Vibrio sinaloensis. The isolated V. sinaloensis strain T47 was confirmed to produce N-butanoyl-L-homoserine lactone (C4-HSL) by using high resolution liquid chromatography tandem mass spectrometry. V. sinaloensis strain T47 also formed biofilms and its biofilm formation could be affected by anti-QS compound (cathechin) suggesting this is a QS-regulated trait in V. sinaloensis strain T47. To our knowledge, this is the first documentation of AHL and biofilm production in V. sinaloensis strain T47.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. acelinetan38@yahoo.com.
authors.forename: Pui-Wan
authors.initials: PW
authors.lastname: Tan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. tmarilyn36@gmail.com.
authors.forename: Wen-Si
authors.initials: WS
authors.lastname: Tan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. ninayusrina@hotmail.com.
authors.forename: Nina Yusrina Muhamad
authors.initials: NY
authors.lastname: Yunos
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. zetty_mohamad@yahoo.com.
authors.forename: Nur Izzati
authors.initials: NI
authors.lastname: Mohamad
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. adrian_tan_1991@yahoo.com.
authors.forename: Tan-Guan-Sheng
authors.initials: TG
authors.lastname: Adrian
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. yinwaifong@yahoo.com.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. kokgan@um.edu.my.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D053038:Quorum Sensing / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014733:Vibrio / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: C092312:N-butyrylhomoserine lactone; D012336:RNA, Ribosomal, 16S; D015107:4-Butyrolactone