title: Admission cell free DNA as a prognostic factor in burns: quantification by use of a direct rapid fluorometric technique.
abstract: BACKGROUND
  Despite great advances in the treatment of burn patients, useful prognostic markers are sparse. During the past years there has been increasing interest in circulating plasma cell free DNA as a potential marker for tissue injury. We have developed a rapid direct fluorescent assay for cell free DNA quantification that allows obtaining accurate, fast, and inexpensive measurements.
  
  
  OBJECTIVE
  To use this technique for measuring plasma cell free DNA levels in burn patients and to further explore the use of cell free DNA as a potential marker of patient outcome in burns.
  
  
  METHODS
  Cell free DNA levels obtained from 14 burn victims within 6 hours of injury and 14 healthy controls were quantified by a direct rapid fluorometric assay.
  
  
  RESULTS
  Patient admission cell free DNA levels were significantly elevated compared with that of controls (1797 ± 1523 ng/mL versus 374 ± 245 ng/mL, P = 0.004). There are statistically significant correlations between cell free DNA admission levels and burn degree (Spearman's correlation = 0.78, P = 0.001), total body surface area (Spearman's correlation = 0.61, P = 0.02), and total burn volume (Spearman's correlation = 0.64, P = 0.014).
  
  
  CONCLUSIONS
  Admission cell free DNA levels can serve as a prognostic factor in burns and future routine use can be made possible by use of our direct rapid fluorometric assay.
authors.affiliation: Plastic and Reconstructive Surgery Department and Burn Unit, Soroka University Medical Center and Ben-Gurion University of the Negev Faculty of Health Sciences, Beer Sheva 8410100, Israel.
authors.forename: Yaron
authors.identifier: 0000-0002-6222-5657
authors.initials: Y
authors.lastname: Shoham
authors.affiliation: Plastic and Reconstructive Surgery Department and Burn Unit, Soroka University Medical Center and Ben-Gurion University of the Negev Faculty of Health Sciences, Beer Sheva 8410100, Israel.
authors.forename: Yuval
authors.initials: Y
authors.lastname: Krieger
authors.affiliation: Surgery A Department, Soroka University Medical Center and Ben-Gurion University of the Negev Faculty of Health Sciences, Beer Sheva 8410100, Israel.
authors.forename: Zvi H
authors.identifier: 0000-0002-1131-5230
authors.initials: ZH
authors.lastname: Perry
authors.affiliation: Surgery B Department, Soroka University Medical Center and Ben-Gurion University of the Negev Faculty of Health Sciences, Beer Sheva 8410100, Israel.
authors.forename: Gad
authors.initials: G
authors.lastname: Shaked
authors.affiliation: Plastic and Reconstructive Surgery Department and Burn Unit, Soroka University Medical Center and Ben-Gurion University of the Negev Faculty of Health Sciences, Beer Sheva 8410100, Israel.
authors.forename: Alexander
authors.initials: A
authors.lastname: Bogdanov-Berezovsky
authors.affiliation: Plastic and Reconstructive Surgery Department and Burn Unit, Soroka University Medical Center and Ben-Gurion University of the Negev Faculty of Health Sciences, Beer Sheva 8410100, Israel.
authors.forename: Eldad
authors.initials: E
authors.lastname: Silberstein
authors.affiliation: Plastic and Reconstructive Surgery Department and Burn Unit, Soroka University Medical Center and Ben-Gurion University of the Negev Faculty of Health Sciences, Beer Sheva 8410100, Israel.
authors.forename: Amiram
authors.initials: A
authors.lastname: Sagi
authors.affiliation: Clinical Biochemistry Department, Soroka University Medical Center and Ben-Gurion University of the Negev Faculty of Health Sciences, Beer Sheva 8410100, Israel.
authors.forename: Amos
authors.initials: A
authors.lastname: Douvdevani
mesh_terms: D000328:Adult; D002056:Burns / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002474:Cell-Free System; D004247:DNA / Q000235:genetics*; D005260:Female; D005470:Fluorometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis
chemical_list: D004247:DNA