title: Organophosphate flame retardants and plasticizers in the air and dust in German daycare centers and human biomonitoring in visiting children (LUPE 3).
abstract: Organophosphate (OP) flame retardants and plasticizers are chemicals that have been used in large quantities in diverse consumer and building-related products for decades. In the present study, OPs were measured in paired indoor air and dust samples from 63 daycare centers in Germany. Moreover, the urine of 312 children between 22 and 80 months old who attend these facilities was analyzed for the presence of eight OP metabolites. Tri-(2-butoxyethyl)-phosphate (TBEP), tris-(2-chloroisopropyl) phosphate (TCPP), and tri-n-butyl-phosphate (TnBP) were present in low concentrations in indoor air, with median values of 49 ng/m(3), 2.7 ng/m(3), and 2.2 ng/m(3), respectively. In dust, median values of 225 mg/kg for TBEP, 2.7 mg/kg for TCPP, 1.1mg/kg for diphenyl(2-ethylhexyl) phosphate, and 0.5mg/kg for tri-phenyl-phosphate (TPhP) were found. In the urine samples, the metabolites di-phenyl-phosphate, di-n-butyl-phosphate, and di-(2-butoxyethyl)-phosphate had median values (95th percentiles) of 0.8 μg/l (4.0 μg/l), 0.2 μg/l (0.9 μg/l), and 2.0 μg/l (10.7 μg/l), respectively. A significant correlation was found between the dust and air samples in the levels of TnBP, tris(2-chloroethyl) phosphate (TCEP), and TBEP. For TCEP and TBEP, significant correlations were also observed between the levels in dust and the respective metabolite levels in urine. For TCEP, there was also a significant correlation between the concentration in indoor air and metabolite levels in urine. Based on the 95th percentile in dust and air in our study and data from residences in a previously published study, the daily intake of the most abundant OP (TBEP) is high (i.e., 3.2 μg/kg b.w.). This level is approximately 6.4% of the reference dose (RfD) established by the NSF, U.S.A. Overall, our study shows that daycare centers are indoor environments that contribute to OP exposure.
authors.affiliation: Bavarian Health and Food Safety Authority, Department of Chemical Safety and Toxicology, D-80538 Munich, Germany. Electronic address: hermann.fromme@lgl.bayern.de.
authors.forename: H
authors.initials: H
authors.lastname: Fromme
authors.affiliation: Berlin-Brandenburg State Laboratory, Department of Environmental Health Protection, Invalidenstr. 60, D-10557 Berlin, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Lahrz
authors.affiliation: North Rhine-Westphalia State Agency for Nature, Environment and Consumer Protection, D-45659 Recklinghausen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kraft
authors.affiliation: Bavarian Health and Food Safety Authority, Department of Chemical Safety and Toxicology, D-80538 Munich, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Fembacher
authors.affiliation: Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen-Nuremberg, D-91054 Erlangen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Mach
authors.affiliation: Bavarian Health and Food Safety Authority, Department of Chemical Safety and Toxicology, D-80538 Munich, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Dietrich
authors.affiliation: Berlin-Brandenburg State Laboratory, Department of Environmental Health Protection, Invalidenstr. 60, D-10557 Berlin, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Burkardt
authors.affiliation: Bavarian Health and Food Safety Authority, Department of Chemical Safety and Toxicology, D-80538 Munich, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Völkel
authors.affiliation: Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen-Nuremberg, D-91054 Erlangen, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Göen
mesh_terms: D016902:Air Pollution, Indoor / Q000032:analysis*; D002648:Child; D002656:Child Day Care Centers / Q000706:statistics & numerical data*; D002675:Child, Preschool; D004391:Dust / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods / Q000706:statistics & numerical data*; D005260:Female; D005411:Flame Retardants / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005858:Germany; D006801:Humans; D007223:Infant; D008297:Male; D010755:Organophosphates / Q000032:analysis* / Q000652:urine; D010968:Plasticizers / Q000032:analysis*; D018709:Statistics, Nonparametric
chemical_list: D004391:Dust; D005411:Flame Retardants; D010755:Organophosphates; D010968:Plasticizers
keywords: Flame retardant; Human biomonitoring; Indoor air; Metabolite; TBEP; TCEP