title: In vitro antimicrobial activity of Medilox® super-oxidized water.
abstract: OBJECTIVE
  Super-oxidized water is one of the broad spectrum disinfectants, which was introduced recently. There are many researches to find reliable chemicals which are effective, inexpensive, easy to obtain and use, and effective for disinfection of microorganisms leading hospital infections. Antimicrobial activity of super-oxidized water is promising. The aim of this study was to investigate the in-vitro antimicrobial activity of different concentrations of Medilox® super-oxidized water that is approved by the Food and Drug Administration (FDA) as high level disinfectant.
  
  
  METHODS
  In this study, super-oxidized water obtained from Medilox® [Soosan E & C, Korea] device, which had been already installed in our hospital, was used. Antimicrobial activities of different concentrations of super-oxidized water (1/1, 1/2, 1/5, 1/10, 1/20, 1/50, 1/100) at different exposure times (1, 2, 5, 10, 30 min) against six ATCC strains, eight antibiotic resistant bacteria, yeasts and molds were evaluated using qualitative suspension test. Dey-Engley Neutralizing Broth [Sigma-Aldrich, USA] was used as neutralizing agent.
  
  
  RESULTS
  Medilox® was found to be effective against all standard strains (Acinetobacter baumannii 19606, Escherichia coli 25922, Enterococcus faecalis 29212, Klebsiella pneumoniae 254988, Pseudomonas aeruginosa 27853, Staphylococcus aureus 29213), all clinical isolates (Acinetobacter baumannii, Escherichia coli, vancomycin-resistant Enterococcus faecium, Klebsiella pneumoniae, Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, Bacillus subtilis, Myroides spp.), and all yeastsat 1/1 dilution in ≥1 minute. It was found to be effective on Aspergillus flavus at 1/1 dilution in ≥2 minutes and on certain molds in ≥5 minutes.
  
  
  CONCLUSIONS
  Medilox® super-oxidized water is a broad spectrum, on-site producible disinfectant, which is effective on bacteria and fungi and can be used for the control of nosocomial infection.
authors.affiliation: Department of Medical Microbiology, Istanbul University, Cerrahpasa Medical Faculty, Istanbul, Turkey. murat.gunaydin@istanbul.edu.tr.
authors.forename: Murat
authors.initials: M
authors.lastname: Gunaydin
authors.forename: Saban
authors.initials: S
authors.lastname: Esen
authors.forename: Adil
authors.initials: A
authors.lastname: Karadag
authors.forename: Nevzat
authors.initials: N
authors.lastname: Unal
authors.forename: Keramettin
authors.initials: K
authors.lastname: Yanik
authors.forename: Hakan
authors.initials: H
authors.lastname: Odabasi
authors.forename: Asuman
authors.initials: A
authors.lastname: Birinci
mesh_terms: D001419:Bacteria / Q000187:drug effects*; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005658:Fungi / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D013997:Time Factors; D014481:United States
chemical_list: D004202:Disinfectants; C118085:superoxidized water; D006861:Hydrogen Peroxide