title: Eating habits modulate short term memory and epigenetical regulation of brain derived neurotrophic factor in hippocampus of low- and high running capacity rats.
abstract: Exercise capacity and dietary restriction (DR) are linked to improved quality of life, including enhanced brain function and neuro-protection. Brain derived neurotrophic factor (BDNF) is one of the key proteins involved in the beneficial effects of exercise on brain. Low capacity runner (LCR) and high capacity runner (HCR) rats were subjected to DR in order to investigate the regulation of BDNF. HCR-DR rats out-performed other groups in a passive avoidance test. BDNF content increased significantly in the hippocampus of HCR-DR groups compared to control groups (p<0.05). The acetylation of H3 increased significantly only in the LCR-DR group. However, chip-assay revealed that the specific binding between acetylated histone H3 and BNDF promoter was increased in both LCR-DR and HCR-DR groups. In spite of these increases in binding, at the transcriptional level only, the LCR-DR group showed an increase in BDNF mRNA content. Additionally, DR also induced the activity of cAMP response element-binding protein (CREB), while the content of SIRT1 was not altered. Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) was elevated in HCR-DR groups. But, based on the levels of nuclear respiratory factor-1 and cytocrome c oxidase, it appears that DR did not cause mitochondrial biogenesis. The data suggest that DR-mediated induction of BDNF levels includes chromatin remodeling. Moreover, DR does not induce mitochondrial biogenesis in the hippocampus of LCR/HCR rats. DR results in different responses to a passive avoidance test, and BDNF regulation in LCR and HCR rats.
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Torma
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Bori
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Erika
authors.initials: E
authors.lastname: Koltai
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Klara
authors.initials: K
authors.lastname: Felszeghy
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Vacz
authors.affiliation: Department of Anesthesiology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Lauren
authors.initials: L
authors.lastname: Koch
authors.affiliation: Department of Anesthesiology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Britton
authors.affiliation: Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA.
authors.forename: Istvan
authors.initials: I
authors.lastname: Boldogh
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary. Electronic address: radak@tf.hu.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Radak
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D050882:CREB-Binding Protein / Q000378:metabolism; D044127:Epigenesis, Genetic; D005247:Feeding Behavior / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D008297:Male; D008570:Memory, Short-Term / Q000502:physiology*; D051264:Nuclear Respiratory Factors / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D051381:Rats; D012420:Running / Q000502:physiology*; D056564:Sirtuin 1 / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D006657:Histones; D051264:Nuclear Respiratory Factors; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; D014157:Transcription Factors; D050882:CREB-Binding Protein; C494365:Crebbp protein, rat; C488368:Sirt1 protein, rat; D056564:Sirtuin 1
keywords: Brain-derived neurotrophic factor; Dietary restriction; Epigenetics; Exercise capacity; Histone