title: In vivo co-localization of enzymes on RNA scaffolds increases metabolic production in a geometrically dependent manner.
abstract: Co-localization of biochemical processes plays a key role in the directional control of metabolic fluxes toward specific products in cells. Here, we employ in vivo scaffolds made of RNA that can bind engineered proteins fused to specific RNA binding domains. This allows proteins to be co-localized on RNA scaffolds inside living Escherichia coli. We assembled a library of eight aptamers and corresponding RNA binding domains fused to partial fragments of fluorescent proteins. New scaffold designs could co-localize split green fluorescent protein fragments to produce activity as measured by cell-based fluorescence. The scaffolds consisted of either single bivalent RNAs or RNAs designed to polymerize in one or two dimensions. The new scaffolds were used to increase metabolic output from a two-enzyme pentadecane production pathway that contains a fatty aldehyde intermediate, as well as three and four enzymes in the succinate production pathway. Pentadecane synthesis depended on the geometry of enzymes on the scaffold, as determined through systematic reorientation of the acyl-ACP reductase fusion by rotation via addition of base pairs to its cognate RNA aptamer. Together, these data suggest that intra-cellular scaffolding of enzymatic reactions may enhance the direct channeling of a variety of substrates.
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA Wyss Institute for Biologically Inspired Engineering, 3 Blackfan St., Boston, MA 02115, USA.
authors.forename: Gairik
authors.initials: G
authors.lastname: Sachdeva
authors.affiliation: Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Garg
authors.affiliation: Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
authors.forename: David
authors.initials: D
authors.lastname: Godding
authors.affiliation: Wyss Institute for Biologically Inspired Engineering, 3 Blackfan St., Boston, MA 02115, USA.
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Way
authors.affiliation: Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA Wyss Institute for Biologically Inspired Engineering, 3 Blackfan St., Boston, MA 02115, USA pamela_silver@hms.harvard.edu.
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Silver
mesh_terms: D000473:Alkanes / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins / Q000235:genetics; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D000473:Alkanes; D052157:Aptamers, Nucleotide; D004798:Enzymes; D005456:Fluorescent Dyes; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C033245:pentadecane; D012313:RNA; D019802:Succinic Acid