title: Cloning, expression, and characterization of miR058 and its target PPO during the development of grapevine berry stone.
abstract: Polyphenol oxidases catalyzing the oxygen-dependent oxidation of phenols to quinones are ubiquitous among angiosperms. They are key enzymes playing a significant role during the synthesis of lignin. The inhibition of the synthesis of lignin in grapevine can cause seedless grapevine berry development. In this study, grapevine PPO (Vv-PPO) was predicted as the target gene of Vv-miR058 by bioinformatics analysis, and it was further cloned and its homologous conservation in various plants was analyzed. The expression profiles of miR058 and its target Vv-PPO were detected by qRT-PCR in peel, pulp and seeds of three grapevine cultivars and Vv-PPO was expressed in an opposite variation way with Vv-miR058 where both of them could be detected, suggesting that Vv-miR058 can play an important role by regulating the expression of Vv-PPO. In addition, the potential target gene Vv-PPO for Vv-miR058 was verified by RLM-RACE. This result would be helpful in theoretical basis for further research and seedless grapevine berry production.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Ren
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Baoju
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Mu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: wangchen@njau.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Tao
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jinggui
authors.initials: J
authors.lastname: Fang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004156:Catechol Oxidase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D035264:Flowers / Q000235:genetics; D005638:Fruit / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008031:Lignin / Q000096:biosynthesis; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D022821:RNA Splice Sites / Q000235:genetics; D016415:Sequence Alignment; D027843:Vitis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D010940:Plant Proteins; D022821:RNA Splice Sites; D008031:Lignin; D004156:Catechol Oxidase
keywords: Grapevine berry stone; MicroRNA; Target genes