title: Protective effects of bifidobacterial adhesin on intestinal mucosa of stressed male rats via modulation of inflammation.
abstract: This study aimed to assess BA impact on inflammation markers and repair of intestinal mucosa. Forty-eight rats were randomly divided into stress (n = 24) and BA (n = 24) groups. Stress was induced by fettering in all animals, fed enterally with 125.4 kJ/kg/d and 0.2 g/kg/d nitrogen. Then, rats were treated for 8 days with 5 mg/kg/d BA (BA group) or 5 mg/kg/d saline (Stress group). Levels of NF-κB, IL-10, TNF-α, and IFN-γ were measured at different time points, in plasma and intestinal mucosa samples. Changes in intestinal mucosa morphology were observed by electron microscopy. Plasma and/or mucosal levels of NF-κB, TNF-α, and IFN-γ were significantly higher in both groups after stress induction (P < 0.05). These high levels persisted in control animals throughout the experiment, and were significantly reduced in the BA group, 3 and 8 days after stress induction (P < 0.05). Interestingly, IL-10 levels were increased after BA treatment (P < 0.05). At day 8, ileal mucosal villi and crypt structure were significantly restored in the BA group. Bifidobacterial adhesin plays a role in repairing intestinal mucosa injury after stress by regulating the release of inflammatory mediators in the intestinal mucosa.
authors.affiliation: Department of Nutrition, East Hospital, Tong Ji University School of Medicine Shanghai, 200000, China.
authors.forename: Xiao-Liang
authors.initials: XL
authors.lastname: Shu
authors.affiliation: Department of Nutrition, East Hospital, Tong Ji University School of Medicine Shanghai, 200000, China.
authors.forename: Tin-Tin
authors.initials: TT
authors.lastname: Yu
authors.affiliation: Department of Nutrition, East Hospital, Tong Ji University School of Medicine Shanghai, 200000, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Kang
authors.affiliation: Department of Nutrition, East Hospital, Tong Ji University School of Medicine Shanghai, 200000, China.
authors.forename: Han
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Endorine, Tong Ji Hospital, Tong Ji University School of Medicine Shanghai, 200000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lei
mesh_terms: D018829:Adhesins, Bacterial / Q000494:pharmacology*; D000818:Animals; D001644:Bifidobacterium; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism / Q000473:pathology*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000150:complications*
chemical_list: D018829:Adhesins, Bacterial
keywords: Bifidobacterial adhesion; NF-κB; cytokines; intestinal mucosa; restrain stress