title: Volatile compounds in cryptic species of the Aneura pinguis complex and Aneura maxima (Marchantiophyta, Metzgeriidae).
abstract: Aneura pinguis is one of the liverwort species complexes that consist of several cryptic species. Ten samples collected from different regions in Poland are in the focus of our research. Eight of the A. pinguis complex belonging to four cryptic species (A, B, C, E) and two samples of closely related species Aneura maxima were tested for the composition of volatile compounds. The HS-SPME technique coupled to GC/FID and GC/MS analysis has been applied. The fiber coated with DVB/CAR/PDMS has been used. The results of the present study, revealed the qualitative and quantitative differences in the composition of the volatile compounds between the studied species. Mainly they are from the group of sesquiterpenoids, oxygenated sesquiterpenoids and aliphatic hydrocarbons. The statistical methods (CA and PCA) showed that detected volatile compounds allow to distinguish cryptic species of A. pinguis. All examined cryptic species of the A. pinguis complex differ from A. maxima. Species A and E of A. pinguis, in CA and PCA, form separate clusters remote from two remaining cryptic species of A. pinguis (B and C) and A. maxima. Relationship between the cryptic species appeared from the chemical studies are in accordance with that revealed on the basis of DNA sequences.
authors.affiliation: Faculty of Chemistry, Adam Mickiewicz University, Umultowska 89b, 61-614 Poznań, Poland. Electronic address: rafwawrz@amu.edu.pl.
authors.forename: Rafał
authors.initials: R
authors.lastname: Wawrzyniak
authors.affiliation: Faculty of Chemistry, Adam Mickiewicz University, Umultowska 89b, 61-614 Poznań, Poland.
authors.forename: Wiesław
authors.initials: W
authors.lastname: Wasiak
authors.affiliation: Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Bączkiewicz
authors.affiliation: Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Buczkowska
mesh_terms: D004129:Dimethylpolysiloxanes; D008401:Gas Chromatography-Mass Spectrometry; D035926:Hepatophyta / Q000737:chemistry* / Q000235:genetics; D006838:Hydrocarbons; D015394:Molecular Structure; D011145:Polyvinyls; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004129:Dimethylpolysiloxanes; D006838:Hydrocarbons; D011145:Polyvinyls; D013729:Terpenes; D055549:Volatile Organic Compounds; C445089:poly(dimethylsiloxane-divinylbenzene)
keywords: Aneura maxima; Aneura pinguis; CA; Cryptic species; DNA sequences; GC/MS; HS-SPME; Liverworts; PCA; Terpenoids