title: Administered chrysanthemum flower oil attenuates hyperuricemia: mechanism of action as revealed by DNA microarray analysis.
abstract: We applied Chrysanthemum flower oil (CFO) to a hyperuricemia model by feeding rats a hyperuricemia-inducing diet (HID) and investigated its effect on serum uric acid (SUA) levels and its mode of action. CFO is the oily fraction that contains polyphenols derived from chrysanthemum flowers. Oral administration of CFO to HID-fed rats significantly decreased their SUA levels. It also inhibited xanthine oxidase activities in the liver and increased urine uric acid levels. The effects of CFO on the renal gene expressions that accompanied the induction of hyperuricemia were comprehensively confirmed by DNA microarray analysis. The analysis showed up-regulation of those genes for uric acid excretion by CFO administration. These results suggest that CFO suppresses the increase in SUA levels via two mechanisms: suppression of uric acid production by inhibition of xanthine oxidase in the liver and acceleration of its excretion by up-regulation of uric acid transporter genes in the kidney.
authors.affiliation: a Frontier Biochemical & Medical Research Laboratories, Kaneka Corporation , Takasago , Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Honda
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawamoto
authors.forename: Hozumi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kishida
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakai
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.forename: Dai
authors.initials: D
authors.lastname: Hirata
mesh_terms: D000818:Animals; D002417:Cattle; D031202:Chrysanthemum / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry*; D033461:Hyperuricemia / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D014527:Uric Acid / Q000097:blood; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D010938:Plant Oils; D014527:Uric Acid; D014969:Xanthine Oxidase
keywords: DNA microarray; chrysanthemum; hyperuricemia; uric acid; xanthine oxidase