title: Partial sequencing of Bm86 gene for studying the phylogeny of an Indian isolate of Rhipicephalus (Boophilus) microplus tick.
abstract: Tick gut glycoprotein, designated as Bm86, found on the luminal surface of the plasma membrane of gut epithelial cells of Boophilus microplus, which is a concealed antigen, has been used as vaccine candidate molecule for immunization against ticks. To better understand the molecular diversity of Bm86 gene in ticks, a portion of the cDNA was sequenced from an Indian isolate of B. microplus. Comparison of nucleotide sequence revealed that Indian isolate had 97 % homology (18 polymorphisms) with that of the Australian isolate and 96 % homology (20 polymorphisms) with that of the Cuban vaccine strain. Further, the Indian isolate differed from the Cuban vaccine isolate at 7 amino acid loci, including 5 substitutions (at residues 88, 94, 175, 176 and 177) and 2 deletions (at 183 and 184). However, protein prediction studies did not show any difference in the putative antigenic epitopes of the protein expressed.
authors.affiliation: Department of Veterinary Parasitology, Veterinary College and Research Institute, Namakkal, 2 India.
authors.forename: P
authors.initials: P
authors.lastname: Anbarasi
authors.affiliation: Department of Veterinary Parasitology, Madras Veterinary College, TANUVAS, Chennai, 600007 India.
authors.forename: B R
authors.initials: BR
authors.lastname: Latha
authors.affiliation: Department of Bio-technology, Madras Veterinary College, TANUVAS, Chennai, 600007 India.
authors.forename: G
authors.initials: G
authors.lastname: Dhinakar Raj
authors.affiliation: Livestock Research Station, TANUVAS, Chennai, 600007 India.
authors.forename: C
authors.initials: C
authors.lastname: Sreekumar
authors.affiliation: Madras Veterinary College, TANUVAS, Chennai, 600007 India.
authors.forename: S
authors.initials: S
authors.lastname: Senthuran
keywords: Bm86; Concealed antigen; Phylogenetic tree; Rhipicephalus microplus; Tick vaccine