title: Pressure effects on the chimeric 3-isopropylmalate dehydrogenases of the deep-sea piezophilic Shewanella benthica and the atmospheric pressure-adapted Shewanella oneidensis.
abstract: The chimeric 3-isopropylmalate dehydrogenase enzymes were constructed from the deep-sea piezophilic Shewanella benthica and the shallow water Shewanella oneidensis genes. The properties of the enzymatic activities under pressure conditions indicated that the central region, which contained the active center and the dimer forming domains, was shown to be the most important region for pressure tolerance in the deep-sea enzyme.
authors.affiliation: a Department of Life Science , Rikkyo University , Tokyo , Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hamajima
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nagae
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Watanabe
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kato-Yamada
authors.forename: Takeo
authors.initials: T
authors.lastname: Imai
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kato
mesh_terms: D050539:3-Isopropylmalate Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics*; D011312:Pressure; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012623:Seawater; D020592:Shewanella / Q000201:enzymology / Q000502:physiology*
chemical_list: D011993:Recombinant Fusion Proteins; D050539:3-Isopropylmalate Dehydrogenase
keywords: Shewanella; chimeric enzyme; high pressure; isopropylmalate dehydrogenase; piezophilic bacteria