title: Optimization of enzyme assisted extraction of Fructus Mori polysaccharides and its activities on antioxidant and alcohol dehydrogenase.
abstract: In the present study, enzyme assisted extraction of Fructus Mori polysaccharides (FMPS) from F. mori using four kinds of enzymes and three compound enzymes were examined. Research found that glucose oxidase offered a better performance in enhancement of the extraction yields of FMPS, antioxidant and activate alcohol dehydrogenase activities. The glucose oxidase assisted extraction process was further optimized by using response surface method (RSM) to obtain maximum yield of crude FMPS. The results showed that optimized extraction conditions were ratio of enzyme amount 0.40%, enzyme treated time 38 min, treated temperature 58 °C and liquid-solid radio 11.0. Under these conditions, the mean experimental value of extraction yield (16.16 ± 0.14%) corresponded well with the predicted values and increased 160% than none enzyme treated ones. Pharmacological verification tests showed that F. mori crude polysaccharides had good antioxidant and activate alcohol dehydrogenase activities in vitro.
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, 116 Baoshan North Road, Guiyang, Guizhou 550001, PR China; The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, 116 Baoshan North Road, Guiyang, Guizhou 550001, PR China.
authors.forename: Qingfang
authors.initials: Q
authors.lastname: Deng
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, 116 Baoshan North Road, Guiyang, Guizhou 550001, PR China; The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, 116 Baoshan North Road, Guiyang, Guizhou 550001, PR China. Electronic address: alice9800@sina.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, 116 Baoshan North Road, Guiyang, Guizhou 550001, PR China; The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, 116 Baoshan North Road, Guiyang, Guizhou 550001, PR China. Electronic address: huaguochen81@gmail.com.
authors.forename: Huaguo
authors.initials: H
authors.lastname: Chen
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005949:Glucose Oxidase / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D000426:Alcohol Dehydrogenase; D005949:Glucose Oxidase
keywords: Activate alcohol dehydrogenase activity; Antioxidant activity; Enzyme assisted extraction; Fructus Mori polysaccharides; Response surface methodology