title: Regioselective glucuronidation of the isoflavone calycosin by human liver microsomes and recombinant human UDP-glucuronosyltransferases.
abstract: Hepatic conjugation plays important roles in systemic exposure and drug interactions of flavonoids. In the present study, the hepatic metabolism of calycosin, a major isoflavone from Astragali Radix, was characterized and the regioselectivity in the predominant glucuronidation pathway was first delineated in human liver microsomes (HLMs) and a panel of recombinant human UDP-glucuronosyltransferases (UGTs). Calycosin underwent major glucuronidation and minor oxidation and sulfation in human liver subcellular fractions. The major glucuronide (G2) of calycosin was isolated and identified as calycosin 3'-glucuronide by NMR analysis, and thus, the minor glucuronide (G1) was tentatively assigned as calycosin 7-glucuronide. The formations of both glucuronides in HLMs fit typical Michaelis-Menten kinetics. HLMs exhibited higher affinity (Km, G2 12.37±1.20 μM vs G1 40.90±5.51 μM) and velocity (Vmax, G2 5.39±0.13 nmol/min/mg protein vs G1 2.80±0.13 nmol/min/mg protein) on G2 formation, leading to the intrinsic clearance of calycosin via 3'-glucuronidation 6 times that through 7-glucuronidation. UGT1A1, 1A3 and 1A10 showed activities on both 3'-OH and 7-OH, whereas UGT1A7, 1A8, 1A9, and 2B7 were only capable of catalyzing 3'-OH glucuronidation of calycosin. Among them, UGT1A9 exhibited the highest activity (Clint, 2169.50 μL/min/mg protein) for 3'-glucuronide formation followed by UGT1A7 (Clint, 396.38 μL/min/mg protein). UGT1A1 showed the highest activity towards 7-OH glucuronidation (Clint, 224.34 μL/min/mg protein), which was comparable to its activity on 3'-OH glucuronidation (Clint, 203.82 μL/min/mg protein). Propofol (UGT1A9 inhibitor) produced a complete inhibition of 3'-glucuronide formation accompanied by an increase of 7-glucuronide in HLMs, while bilirubin (UGT1A1 inhibitor) only partially (∼60%) inhibited the 7-OH glucuronidation. These findings demonstrated the regioselective glucuronidation at the 3'-OH of the isoflavone calycosin in HLMs and shed light on potential drug interactions of calycosin with other UGT1A9 substrates.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China.
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Ruan
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China. Electronic address: ruyan@umac.mo.
authors.forename: Ru
authors.initials: R
authors.lastname: Yan
mesh_terms: D005965:Glucuronates / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006801:Humans; D006900:Hydroxylation; D007529:Isoflavones / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008862:Microsomes, Liver / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D013237:Stereoisomerism; D000090264:UDP-Glucuronosyltransferase 1A9
chemical_list: D005965:Glucuronates; D007529:Isoflavones; D011994:Recombinant Proteins; C000715591:UGT1A9 protein, human; C121707:7,3'-dihydroxy-4'-methoxyisoflavone; D014453:Glucuronosyltransferase; D000090264:UDP-Glucuronosyltransferase 1A9
keywords: Calycosin; Glucuronidation; Metabolism; Regioselectivity; UDP-glucuronosyltransferases