title: Chemopreventive effects of oplopantriol A, a novel compound isolated from Oplopanax horridus, on colorectal cancer.
abstract: Oplopanax horridus is a North American botanical that has received limited investigations. We previously isolated over a dozen of the constituents from O. horridus, and among them oplopantriol A (OPT A) is a novel compound. In this study, we firstly evaluated the in vivo chemoprevention activities of OPT A using the xenograft colon cancer mouse model. Our data showed that this compound significantly suppressed tumor growth with dose-related effects (p < 0.01). Next, we characterized the compound's growth inhibitory effects in human colorectal cancer cell lines HCT-116 and SW-480. With OPT A treatment, these malignant cells were significantly inhibited in both a concentration- and time-dependent manner (both p < 0.01). The IC50 was approximately 5 µM for HCT-116 and 7 µM for SW-480 cells. OPT A significantly induced apoptosis and arrested the cell cycle at the G2/M phase. From further mechanism explorations, our data showed that OPT A significantly upregulated the expression of a cluster of genes, especially the tumor necrosis factor receptor family and caspase family, suggesting that the tumor necrosis factor-related apoptotic pathway plays a key role in OPT A induced apoptosis.
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA. zzhang2@bsd.uchicago.edu.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA. CYuan@dacc.uchicago.edu.
authors.forename: Chunhao
authors.initials: C
authors.lastname: Yu
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA. zhangchunfeng67@163.com.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Zhang
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA. cpuwxd@gmail.com.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Wu
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA. cpuwxd@gmail.com.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Wen
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA. sanderson6@uchicago.edu.
authors.forename: Samantha
authors.initials: S
authors.lastname: Anderson
authors.affiliation: Ben May Department for Cancer Research, Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA. wei@uchicago.edu.
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.affiliation: Institute of Clinical Pharmacology, Central South University, Changsha 410000, China. endeavor34852@aliyun.com.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, and Institute of Chinese Medical Sciences, University of Macau, Macao, China. spli@umac.mo.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Li
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA. cwang@dacc.uchicago.edu.
authors.forename: Chong-Zhi
authors.initials: CZ
authors.lastname: Wang
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA. CYuan@dacc.uchicago.edu.
authors.forename: Chun-Su
authors.initials: CS
authors.lastname: Yuan
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000188:drug therapy; D004195:Disease Models, Animal; D005260:Female; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D028002:Oplopanax / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts