title: Autologous platelet-rich plasma: a biological supplement to enhance adipose-derived mesenchymal stem cell expansion.
abstract: Currently the use of non-autologous cell culture media (e.g., animal-derived or allogeneic serum) for clinical applications of mesenchymal stem cells (MSCs) is criticized by regulatory agencies. Autologous platelet-rich plasma (PRP) is proposed as a safer alternative medium supplement for adipose-derived mesenchymal stem cells (AT-MSC) culture. To study its efficiency on cell proliferation, AT-MSCs were cultured for 10 days in media supplemented with different concentrations of autologous non-activated PRP (nPRP) or thrombin-activated PRP (tPRP) (1-60%). AT-MSC proliferation, cell phenotype, multipotency capacity, and chromosome stability were assessed and compared to AT-MSCs expanded in a classical medium supplemented with 10% of fetal bovine serum (FBS). Culture media supplemented with nPRP showed dose-dependent higher AT-MSC proliferation than did FBS or tPRP. Twenty percent nPRP was the most effective concentration to promote cell proliferation. This condition increased 13.9 times greater AT-MSC number in comparison to culture with FBS, without changing the AT-MSC phenotype, differentiation capacity, and chromosome status. We concluded that 20% autologous nPRP is a safe, efficient, and cost-effective supplement for AT-MSC expansion. It should be considered as an alternative to FBS or other nonautologous blood derivatives. It could serve as a potent substitute for the validation of future clinical protocols as it respects good manufacturing practices and regulatory agencies' standards.
authors.affiliation: 1 Department of Plastic, Reconstructive and Aesthetic Surgery, University Hospitals of Geneva (HUG) , Faculty of Medicine, Geneva University, Geneva, Switzerland .
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Atashi
authors.forename: Marisa E E
authors.initials: ME
authors.lastname: Jaconi
authors.forename: Brigitte
authors.initials: B
authors.lastname: Pittet-Cuénod
authors.forename: Ali
authors.initials: A
authors.lastname: Modarressi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D001792:Blood Platelets / Q000166:cytology; D002452:Cell Count; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D013194:Staining and Labeling; D013997:Time Factors; D014182:Transplantation, Autologous
chemical_list: D015415:Biomarkers