title: Identification and characterization of an oocyte factor required for sperm decondensation in pig.
abstract: Mammalian oocytes possess factors to support fertilization and embryonic development, but knowledge on these oocyte-specific factors is limited. In the current study, we demonstrated that porcine oocytes with the first polar body collected at 33 h of in vitro maturation sustain IVF with higher sperm decondensation and pronuclear formation rates and support in vitro development with higher cleavage and blastocyst rates, compared with those collected at 42 h (P<0.05). Proteomic analysis performed to clarify the mechanisms underlying the differences in developmental competence between oocytes collected at 33 and 42 h led to the identification of 18 differentially expressed proteins, among which protein disulfide isomerase associated 3 (PDIA3) was selected for further study. Inhibition of maternal PDIA3 via antibody injection disrupted sperm decondensation; conversely, overexpression of PDIA3 in oocytes improved sperm decondensation. In addition, sperm decondensation failure in PDIA3 antibody-injected oocytes was rescued by dithiothreitol, a commonly used disulfide bond reducer. Our results collectively report that maternal PDIA3 plays a crucial role in sperm decondensation by reducing protamine disulfide bonds in porcine oocytes, supporting its utility as a potential tool for oocyte selection in assisted reproduction techniques.
authors.affiliation: Laboratory of Embryo BiotechnologyCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, ChinaShandong Academy of Agricultural SciencesDairy Cattle Research Center, Jinan, Shandong Province 250100, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory of Embryo BiotechnologyCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, ChinaShandong Academy of Agricultural SciencesDairy Cattle Research Center, Jinan, Shandong Province 250100, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Huan
authors.affiliation: Laboratory of Embryo BiotechnologyCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, ChinaShandong Academy of Agricultural SciencesDairy Cattle Research Center, Jinan, Shandong Province 250100, China.
authors.forename: Bingteng
authors.initials: B
authors.lastname: Xie
authors.affiliation: Laboratory of Embryo BiotechnologyCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, ChinaShandong Academy of Agricultural SciencesDairy Cattle Research Center, Jinan, Shandong Province 250100, China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Embryo BiotechnologyCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, ChinaShandong Academy of Agricultural SciencesDairy Cattle Research Center, Jinan, Shandong Province 250100, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Laboratory of Embryo BiotechnologyCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, ChinaShandong Academy of Agricultural SciencesDairy Cattle Research Center, Jinan, Shandong Province 250100, China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Jiao
authors.affiliation: Laboratory of Embryo BiotechnologyCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, ChinaShandong Academy of Agricultural SciencesDairy Cattle Research Center, Jinan, Shandong Province 250100, China.
authors.forename: Tianqing
authors.initials: T
authors.lastname: Huang
authors.affiliation: Laboratory of Embryo BiotechnologyCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, ChinaShandong Academy of Agricultural SciencesDairy Cattle Research Center, Jinan, Shandong Province 250100, China liu086@126.com kqr086@yahoo.com.
authors.forename: Qingran
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Laboratory of Embryo BiotechnologyCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, ChinaShandong Academy of Agricultural SciencesDairy Cattle Research Center, Jinan, Shandong Province 250100, China liu086@126.com kqr086@yahoo.com.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D059471:In Vitro Oocyte Maturation Techniques; D008297:Male; D009865:Oocytes / Q000201:enzymology*; D019899:Paracrine Communication; D019704:Protein Disulfide-Isomerases / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D020554:Sperm Injections, Intracytoplasmic; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000201:enzymology*; D013552:Swine; D013997:Time Factors; D014162:Transfection
chemical_list: D012333:RNA, Messenger; C513195:Pdia3 protein, mouse; D019704:Protein Disulfide-Isomerases