title: A design for the control of apoptosis in genetically modified Saccharomyces cerevisiae.
abstract: We have engineered a system that holds potential for use as a safety switch in genetically modified yeasts. Human apoptotic factor BAX (no homolog in yeast), under the control of the FBP1 (gluconeogenesis enzyme) promoter, was conditionally expressed to induce yeast cell apoptosis after glucose depletion. Such systems might prove useful for the safe use of genetically modified organisms.
authors.affiliation: a Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Nishida
authors.forename: Misa
authors.initials: M
authors.lastname: Noguchi
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D006597:Fructose-Bisphosphatase / Q000235:genetics; D005818:Genetic Engineering; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D051028:bcl-2-Associated X Protein; C111172:FBP1 protein, S cerevisiae; D006597:Fructose-Bisphosphatase
keywords: Bax; Saccharomyces cerevisiae; apoptosis; genetically modified organism (GMO); gluconeogenesis