title: Complete genome sequence of Salmonella enterica serovar Typhimurium VNP20009, a strain engineered for tumor targeting.
abstract: A mutagenized and genetically modified derivative of Salmonella enterica serovar Typhimurium 14028S, VNP20009 (ATCC 202165), is attenuated in host virulence and accumulates preferentially in tumors. Here, we report the complete genome of this anticancer therapy agent consisting of one chromosome and one virulence plasmid. The major genetic features that distinguish VNP20009 from its parental strain are: an engineered msbB deletion, a 3'-extension in pykA, a Tn10-caused 16.6-kbp inversion leading to the disruption of purM, a 108-kbp Suwwan deletion resulting in the loss of 128 genes, and 50 non-synonymous SNPs.
authors.affiliation: Department of Biological Sciences, Life Sciences I, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Broadway
authors.affiliation: Department of Biological Sciences, Life Sciences I, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors.forename: Thero
authors.initials: T
authors.lastname: Modise
authors.affiliation: Department of Biological Sciences, Life Sciences I, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors.forename: Roderick V
authors.initials: RV
authors.lastname: Jensen
authors.affiliation: Department of Biological Sciences, Life Sciences I, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. Electronic address: bscharf@vt.edu.
authors.forename: Birgit E
authors.initials: BE
authors.lastname: Scharf
mesh_terms: D001483:Base Sequence; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009369:Neoplasms; D012486:Salmonella typhimurium / Q000235:genetics*; D017422:Sequence Analysis, DNA; D065288:Serogroup
keywords: Anticancer therapy; Attenuated; Auxotrophic; Phase I clinical trial; Tumor colonization