title: Automated analytical standard production with supercritical fluid chromatography for the quantification of bioactive C17-polyacetylenes: a case study on food processing waste.
abstract: Food processing enterprises produce enormous amounts of organic waste that contains valuable phytochemicals (e.g. C17-polyacetylenes). Knowledge on the phytochemicals content is a first step towards valorisation. Quantification of C17-polyacetylenes is however often hampered by the lack of commercially available standards or by tedious multistep in-house standard production procedures. In the current study, a new and straightforward supercritical fluid chromatography purification procedure is described for the simultaneous production of 2 analytical C17-polyacetylene standards. Respectively, 5 and 6 mg of falcarinol and falcarindiol were purified in 17 h on analytical scale. After confirming the identity and quality (97% purity) by Nuclear Magnetic Resonance, accurate mass-Mass Spectrometry (am-MS) and Photo Diode Array (PDA) detection the C17-polyacetylene standards were used for the analysis of industrial vegetable waste with Liquid Chromatography coupled to PDA and am-MS detection. Measurements showed varying concentrations of C17-polyacetylenes in the organic waste depending on its nature and origin.
authors.affiliation: Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology (SCT), Boeretang 200, 2400 Mol, Belgium; University of Antwerp, NatuRA, Universiteitsplein 1, 2610 Antwerp, Belgium. Electronic address: sebastiaan.bijttebier@vito.be.
authors.forename: Sebastiaan
authors.initials: S
authors.lastname: Bijttebier
authors.affiliation: Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology (SCT), Boeretang 200, 2400 Mol, Belgium. Electronic address: els.dhondt@vito.be.
authors.forename: Els
authors.initials: E
authors.lastname: D'Hondt
authors.affiliation: Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology (SCT), Boeretang 200, 2400 Mol, Belgium. Electronic address: bart.noten@vito.be.
authors.forename: Bart
authors.initials: B
authors.lastname: Noten
authors.affiliation: University of Antwerp, NatuRA, Universiteitsplein 1, 2610 Antwerp, Belgium. Electronic address: nina.hermans@uantwerpen.be.
authors.forename: Nina
authors.initials: N
authors.lastname: Hermans
authors.affiliation: University of Antwerp, NatuRA, Universiteitsplein 1, 2610 Antwerp, Belgium. Electronic address: sandra.apers@uantwerpen.be.
authors.forename: Sandra
authors.initials: S
authors.lastname: Apers
authors.affiliation: University of Antwerp, NatuRA, Universiteitsplein 1, 2610 Antwerp, Belgium. Electronic address: vasiliki.exarchou@uantwerpen.be.
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Exarchou
authors.affiliation: Flemish Institute for Technological Research (VITO), Business Unit Separation and Conversion Technology (SCT), Boeretang 200, 2400 Mol, Belgium. Electronic address: stefan.voorspoels@vito.be.
authors.forename: Stefan
authors.initials: S
authors.lastname: Voorspoels
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D005511:Food Handling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D053279:Polyynes / Q000032:analysis*
chemical_list: D053279:Polyynes
keywords: C17-polyacetylenes; Carotenoids; Food processing waste; LC-PDA–am-MS; Purification; Semi-prep SFC