title: Heavy ion radiation can promote greater motility and enolase protein expression in ram sperm in in vitro liquid storage.
abstract: The aim of the study was to determine the effects of heavy ion radiation (HIR) on ram sperm quality during 24h of in vitro liquid storage at 15°C, and identify the most appropriate dose which did not injure, but actually improved sperm quality and confirmed the relationship between highly expressed enolase and ram sperm quality during storage in vitro. Six Dorset ram (Ovis aries) semen pools from five mature and healthy rams were each divided into seven experimental groups with different doses of HIR (0, 0.05, 0.1, 0.2, 0.3, 0.4, and 0.5Gy) under the same experimental conditions. Sperm motility, viability, ATP content, and the gene and protein expression of enolase were measured at 24h of storage. Irradiated semen which had been stored for 24h, retained not only greater sperm motility, viability, and ATP content, but had greater enolase protein expression. This was evidenced by increased amounts of mRNA for this enzyme and amount of enolase protein as compared with semen from control rams, especially for the 0.1Gy group (P<0.001). These results indicate that HIR can promote enhanced motility and viability during in vitro liquid storage, and the 0.1Gy may be a suitable dose for improving sperm quality. Greater amounts of enolase and ATP content may results from enhanced sperm glycolysis by HIR. HIR enhances sperm glycolysis to generate sufficient ATP for maintaining sperm motility during storage.
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Lanzhou Agricultural Science and Technology Research Promotion Center, Lanzhou 730030, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. Electronic address: zhaoxx@gsau.edu.cn.
authors.forename: Xingxu
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects; D020450:Heavy Ions; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism*; D011829:Radiation Dosage; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D012756:Sheep / Q000235:genetics / Q000378:metabolism; D013081:Sperm Motility / Q000528:radiation effects*; D013094:Spermatozoa / Q000378:metabolism / Q000528:radiation effects*; D015854:Up-Regulation / Q000528:radiation effects
chemical_list: D000255:Adenosine Triphosphate; D010751:Phosphopyruvate Hydratase
keywords: Heavy ion radiation; Motility; Ram sperm