title: Collagen membrane wrapping around methotrexate-containing calcium-phosphate cement reduces the side effects on soft tissue healing.
abstract: OBJECTIVE
  This study aims to evaluate whether the collagen membrane (membrane) wrapping around the methotrexate (MTX)-containing calcium-phosphate cement (CPC) reduces the side effects on soft tissue healing.
  
  
  METHODS
  In 36 rats, femoral bone defects were created and treated in six groups which were CPC only, CPC and membrane wrapping around, CPC containing 2% MTX, CPC containing 2% MTX and membrane wrapping around, CPC containing 5% MTX, CPC containing 5% MTX and membrane wrapping around.
  
  
  RESULTS
  Histological examinations revealed a statistically significantly improved healing in the connective tissue samples of the CPC containing 5% MTX group wrapped around by membrane compared to those without membrane (p=0.04). However, this was not seen in other groups.
  
  
  CONCLUSIONS
  Membrane wrapping around the CPC containing MTX reduces the side effect of MTX on cellular proliferation at its highest concentration, particularly. Membrane wrapping may allow administration of higher doses of an anti-neoplastic drug which can be more effective.
authors.affiliation: Department of Orthopedics and Traumatology, Akşehir State Hospital, 42550 Akşehir, Konya, Turkey.
authors.forename: Anıl S
authors.initials: AS
authors.lastname: Uzun
authors.forename: Alp
authors.initials: A
authors.lastname: Akman
authors.forename: A Fahir
authors.initials: AF
authors.lastname: Demirkan
authors.forename: Semih
authors.initials: S
authors.lastname: Akkaya
authors.forename: Gamze Gököz
authors.initials: GG
authors.lastname: Doğu
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000009:adverse effects*; D001843:Bone Cements; D002130:Calcium Phosphates; D003094:Collagen / Q000008:administration & dosage*; D003238:Connective Tissue / Q000473:pathology / Q000502:physiology*; D005269:Femur / Q000601:surgery; D008727:Methotrexate / Q000008:administration & dosage / Q000009:adverse effects*; D051381:Rats; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000964:Antimetabolites, Antineoplastic; D001843:Bone Cements; D002130:Calcium Phosphates; D003094:Collagen; C020243:calcium phosphate; D008727:Methotrexate