title: Stabilization of antimicrobial silver nanoparticles by a polyhydroxyalkanoate obtained from mixed bacterial culture.
abstract: The incorporation of antimicrobials into polymer matrices is a promising technology in the food packaging and biomedical areas. Among the most widely used antimicrobials, silver nanoparticles (AgNPs) have emerged as one of the most researched technologies to prevent microbial outbreaks. However, it is known that AgNPs are rather unstable and present patterns of agglomeration that might limit their application. In this work, AgNPs were produced by chemical reduction in suspensions of an unpurified poly(3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) which was previously obtained from a mixed culture fermentation using a synthetic medium mimicking fermented cheese whey. The synthesis of AgNPs was carried out within the unpurified PHBV suspension (in situ) and by physical mixing (mix). The stability of crystalline and spherical nanoparticles (7±3nm) obtained in situ was found to be stable during at least 40 days. The results suggest that the unpurified PHBV appears to be a very efficient capping agent, preventing agglomeration and, thereby, stabilizing successfully the silver nanoparticles. The in situ obtained AgNP-PHBV materials were also found to exhibit a strong antibacterial activity against Salmonella enterica at low concentration (0.1-1ppm).
authors.affiliation: Novel Materials and Nanotechnology Laboratory, Institute of Agrochemistry and Food Technology (IATA), CSIC, 46980 Valencia, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Castro-Mayorga
authors.affiliation: Novel Materials and Nanotechnology Laboratory, Institute of Agrochemistry and Food Technology (IATA), CSIC, 46980 Valencia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martínez-Abad
authors.affiliation: Novel Materials and Nanotechnology Laboratory, Institute of Agrochemistry and Food Technology (IATA), CSIC, 46980 Valencia, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Fabra
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Catarina
authors.initials: C
authors.lastname: Olivera
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Reis
authors.affiliation: Novel Materials and Nanotechnology Laboratory, Institute of Agrochemistry and Food Technology (IATA), CSIC, 46980 Valencia, Spain. Electronic address: lagaron@iata.csic.es.
authors.forename: J M
authors.initials: JM
authors.lastname: Lagarón
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D004355:Drug Stability; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D011108:Polymers; D019779:Salmonella enterica / Q000187:drug effects / Q000648:ultrastructure; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000890:Anti-Infective Agents; D054813:Polyhydroxyalkanoates; D011108:Polymers; D012834:Silver
keywords: Antimicrobial properties; Polyhydroxyalkanoates; Silver nanoparticles