title: The neutral self-assembling peptide hydrogel SPG-178 as a topical hemostatic agent.
abstract: Conventional self-assembling peptide hydrogels are effective as topical hemostatic agents. However, there is a possibility to harm living tissues due to their low pH. The aim of the present study was to demonstrate the efficacy of SPG-178, a neutral self-assembling peptide hydrogel, as a topical hemostatic agent. First, we measured the bleeding duration of incisions made on rat livers after application of SPG-178 (1.0% w/v), SPG-178 (1.5% w/v), RADA16 (1.0% w/v), and saline (n = 12/group). Second, we observed the bleeding surfaces by transmission electron microscopy immediately after hemostasis. Third, we measured the elastic and viscous responses (G' and G″, respectively) of the hydrogels using a rheometer. Our results showed that bleeding duration was significantly shorter in the SPG-178 group than in the RADA16 group and that there were no significant differences in transmission electron microscopy findings between the groups. The greater the G' value of a hydrogel, the shorter was the bleeding duration. We concluded that SPG-178 is more effective and has several advantages: it is non-biological, transparent, nonadherent, and neutral and can be sterilized by autoclaving.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Okayama Saiseikai General Hospital, Okayama, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Komatsu
authors.affiliation: Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan; Menicon Co., Ltd., Nagoya, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nagai
authors.affiliation: Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Naruse
authors.affiliation: Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kimata
mesh_terms: D000818:Animals; D006470:Hemorrhage / Q000188:drug therapy*; D006490:Hemostatics / Q000627:therapeutic use*; D020100:Hydrogels / Q000627:therapeutic use*; D008297:Male; D010455:Peptides; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D006490:Hemostatics; D020100:Hydrogels; D010455:Peptides