title: Efficient utilization of cassava pulp for succinate production by metabolically engineered Escherichia coli KJ122.
abstract: A metabolically engineered Escherichia coli KJ122 was efficiently utilized for succinate production from cassava pulp during batch separate hydrolysis and fermentation (SHF) under simple anaerobic conditions. Succinate concentration of 41.46 ± 0.05 g/L with yield and productivity of 82.33 ± 0.14 g/100 g dry pulp and 0.84 ± 0.02 g/L/h was obtained. In batch simultaneous saccharification and fermentation (SSF), hydrolysis of 12 % (w/v) cassava pulp with an enzyme loading of 2 % AMG + 3 % Cel (v/w) at pH 6.5 was optimized at 39 °C. Succinate concentration of 80.86 ± 0.49 g/L with a yield of 70.34 ± 0.37 g/100 g dry pulp and a productivity of 0.84 ± 0.01 g/L/h was attained using E. coli KJ122. Fed-batch SSF significantly enhanced succinate concentration to 98.63 ± 0.12 g/L at yield and productivity of 71.64 ± 0.97 g/100 g dry pulp and 1.03 ± 0.01 g/L/h. This result indicated an efficient and economical succinate production from cassava pulp using SHF and SSF by the use of E. coli KJ122.
authors.affiliation: Metabolic Engineering Research Unit, Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-District, Muang District, Nakhon Ratchasima, 30000, Thailand.
authors.forename: Apichai
authors.initials: A
authors.lastname: Sawisit
authors.forename: Sirima Suvarnakuta
authors.initials: SS
authors.lastname: Jantama
authors.forename: Sunthorn
authors.initials: S
authors.lastname: Kanchanatawee
authors.forename: Kaemwich
authors.initials: K
authors.lastname: Jantama
mesh_terms: D018533:Biomass; D019149:Bioreactors; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D002365:Manihot / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D019802:Succinic Acid