title: Development and evaluation of a molecularly imprinted polymer for the detection and cleanup of benzylpenicillin in milk.
abstract: A molecularly imprinted polymer (MIP) was designed for benzylpenicillin via suspension polymerization. The specific absorption of benzylpenicillin to the MIP, applied in a molecularly imprinted solid-phase extraction (MISPE), was compared to the nonspecific binding using a NIP (nonimprinted polymer without a target molecule) in a non-molecularly imprinted solid-phase extraction. This validation was performed successfully in acetonitrile solutions and milk extracts spiked with benzylpenicillin. Significant differences in absorption were observed. In acetonitrile, the recoveries using MISPE (90-95%) were a fraction higher than those in milk extracts (70-80%). The validation revealed the limit of detection and the limit of quantitation for the MISPE application in milk samples to be 0.51 and 1.02 μg/kg, respectively. In addition, comparing the results of the analysis of positive milk samples using MISPE with those using a classic sample preparation step showed a Pearson correlation of 0.989. Finally, cross reactivity tests using other antibiotics showed a certain cross reactivity, but non-β-lactams were barely bound.
authors.affiliation: Technology and Food Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Government of Flanders , Brusselsesteenweg 370, B-9090 Melle, Belgium.
authors.forename: Geert
authors.initials: G
authors.lastname: Van Royen
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
authors.forename: Els
authors.initials: E
authors.lastname: Daeseleire
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D002417:Cattle; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting; D010400:Penicillin G / Q000737:chemistry* / Q000302:isolation & purification; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D010400:Penicillin G