title: Degradation of fucoidans from Sargassum fulvellum and their biological activities.
abstract: A fucoidan extracted from Sargassum fulvellum, was degraded by ultrasound (US) or electron beam (EB) irradiation in the presence of hydrogen peroxide aqueous solution. From the energetic point of view, the most effective method for the fucoidan degradation was found to be EB radiation method in H2O2 with a yield of scission Gs 3.310 × 10(-8)mol/J at the reaction condition of 1.5% hydrogen peroxide and irradiation 2.5 kGy. The degradation took place by the formation of a reactive hydroxyl radical due to the dissociation of H2O2 in the presence of US or EB. The low molecular weight fucoidans (LMWFs) prevented P-selectin binding to Sialyl Lewis X with an IC50 (inhibitory concentration 50) of 20 nM as compared to 400 nM for heparin and 25,000 nM for dextran sulfate. The LMWFs showed no hemolytic activity at concentrations up to 950 μg/ml.
authors.affiliation: Department of Chemical and Biochemical Engineering, Chosun University, Gwangju 501-759, Republic of Korea.
authors.forename: Byung Wook
authors.initials: BW
authors.lastname: Jo
authors.affiliation: Department of Chemical and Biochemical Engineering, Chosun University, Gwangju 501-759, Republic of Korea. Electronic address: sookchoi@chosun.ac.kr.
authors.forename: Soo-Kyung
authors.initials: SK
authors.lastname: Choi
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004583:Electrons; D006461:Hemolysis / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D019007:P-Selectin / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*; D013010:Sonication
chemical_list: D019007:P-Selectin; D011134:Polysaccharides; C007789:fucoidan; D006861:Hydrogen Peroxide
keywords: Degradation; Electron beam irradiation; Fucoidan; Heamolytic activity; P-selectin; Ultrasound