title: Effects of vitamin D on renal fibrosis in diabetic nephropathy model rats.
abstract: This study is to investigate the effects of vitamin D on renal fibrosis in rat diabetic nephropathy models, as well as the changes and interactions in the expressions of renal fibrogenesis- and inflammation-related genes. Rat diabetic nephropathy models were established by high-fat diets, which were subjected to TGF-β1 manipulation, as well as vitamin D treatment. H&E staining, Masson staining, and TEM detection were performed to assess the effects of vitamin D treatment and/or TGF-β1 manipulation on pathological changes in the renal tissues in these rat diabetic nephropathy models. Immunohistology and real-time PCR were used to evaluate the expressions of TGF-β1, MCP-1, CTGF, and VDR. Histological staining and TEM detection showed that, in both TGF-β1 over-expressed and interfered groups, vitamin D administration alleviated the renal fibrosis, compared with the vehicle treatment. Similar results were observed with the immunohistological staining. Real-time PCR analysis indicated that, when TGF-β1 was over-expressed in diabetic nephropathy, the expressions of MCP-1 and CTGF were also up-regulated, which would be decreased by the treatment of vitamin D. On the other hand, when TGF-β1 was interfered in DN, the expressions of MCP-1 and CTGF were relatively down-regulated, which would be further lowered by vitamin D administration. The mRNA expression of VDR was elevated by vitamin D treatment in these diabetic nephropathy models. Active vitamin D3 and lentivirus-mediated TGF-β1 interference could effectively reduce the renal fibrosis and protect the renal function in diabetic nephropathy rat models, which makes a promising therapeutic strategy for the disease.
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Institute of Research, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Lv
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yu
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China.
authors.forename: Lati
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003928:Diabetic Nephropathies / Q000150:complications* / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D005355:Fibrosis / Q000473:pathology; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D046529:Microscopy, Electron, Transmission; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D018167:Receptors, Calcitriol / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014807:Vitamin D / Q000494:pharmacology*
chemical_list: C526316:CCN2 protein, rat; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D018167:Receptors, Calcitriol; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor; D014807:Vitamin D
keywords: CTGF; Diabetic nephropathy; MCP-1; TGF-β1; VDR; vitamin D