title: Proteomics profiling of keratocystic odontogenic tumours reveals AIDA as novel biomarker candidate.
abstract: BACKGROUND
  Keratocystic odontogenic tumour (KCOT) is a benign, yet aggressive odontogenic tumour. Herein, proteome analysis of KCOT lesions in comparison with control patient-matched tissue unaffected by the disease and with inflammatory odontogenic cysts, namely radicular cysts is presented.
  
  
  METHODS
  For the proteomics profiling, two complementary proteomics techniques MALDI-MS/MS and LC-ESI-MS/MS were employed. Potential candidate biomarkers were validated by immunohistochemistry.
  
  
  RESULTS
  More than 43 proteins were found to be differentially expressed or up-regulated in KCOT lesions in comparison with patient-matched unaffected oral mucosa. These proteins bear important biological functions and are involved in cell proliferation, cytoskeletal re-organization, transcription, cellular motility and apoptosis. In particular, a number of differentially expressed proteins participate in autocrine regulation and signalization within JNK and p38 MAPK signalling pathways.
  
  
  CONCLUSIONS
  Immunohistochemical validation of chosen putative biomarkers revealed axin interaction partner and dorsalization-antagonist (AIDA), known as a protein that blocks activation of JNK signalling pathway, as a differential biomarker for KCOT lesions on an independent cohort of KCOT tissue samples in comparison with most prevalent intra-oseal lesions inflammatory odontogenic cysts.
authors.affiliation: Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Ivanišević Malčić
authors.forename: Lucas
authors.initials: L
authors.lastname: Breen
authors.forename: Djuro
authors.initials: D
authors.lastname: Josić
authors.forename: Silvana
authors.initials: S
authors.lastname: Jukić Krmek
authors.forename: Tihana
authors.initials: T
authors.lastname: Džombeta
authors.forename: Jurica
authors.initials: J
authors.lastname: Matijević
authors.forename: Lovro
authors.initials: L
authors.lastname: Grgurević
authors.forename: Krešimir
authors.initials: K
authors.lastname: Pavelić
authors.forename: Božo
authors.initials: B
authors.lastname: Krušlin
authors.forename: Sandra
authors.initials: S
authors.lastname: Kraljević Pavelić
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000502:physiology; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000737:chemistry / Q000378:metabolism; D009808:Odontogenic Tumors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D020543:Proteome
keywords: AIDA; biomarkers; immunohistochemistry; keratocystic odontogenic tumour; mass spectrometry