title: In vitro toxicity evaluation of Ti(4+)-stabilized γ-Bi2O3 sillenites.
abstract: We report results regarding the in vitro toxicology of γ-Bi2O3 represented by its isomorphous phase Bi12TiO20 (γ-BTO). The γ-BTO microparticles were synthesized by two methods: coprecipitation from a bismuth nitrate-tetrabutyl titanate solution and solid state reaction of Bi2O3 and TiO2 oxides. The structural and morphological characteristics of the obtained materials were determined using X-ray diffraction (XRD), selected area electron diffraction (SAED), transmission (TEM) and scanning (SEM) electron microscopy. The elemental composition was investigated using energy dispersive spectrometry (EDS). The cytotoxicity and oxidative/nitrosative stress (intracellular reactive oxygen species (ROS) and nitric oxide (NO) release) induced by the studied microparticles in HepG2, SH-SY5Y and 3T3-L1 cell cultures were determined using the MTT, DCF-DA (2',7'-dichlorfluorescein-diacetate) and Griess methods respectively. Depending on the cell type and γ-BTO concentration, results showed only weak cytotoxic effects after 24h of γ-BTO exposure and cell proliferation effects for longer treatment times. Only reduced NO release increases (corresponding to high γ-BTO concentrations) were detected in case of SH-SY5Y and 3T3-L1 cells. The intracellular ROS production (higher for HepG2 cells) appeared inversely proportional to the γ-BTO concentration. The obtained results indicated a promising in vitro biocompatibility of γ-BTO and encourage further studies regarding its potential for biomedical applications.
authors.affiliation: National Institute of Materials Physics, P.O. Box MG-7, Bucharest, Romania; University of Bucharest, Faculty of Physics, Bucharest, Romania. Electronic address: tr.popescu@gmail.com.
authors.forename: T
authors.initials: T
authors.lastname: Popescu
authors.affiliation: Cantacuzino National Institute of Research and Development for Microbiology and Immunology, Bucharest, Romania.
authors.forename: A R
authors.initials: AR
authors.lastname: Lupu
authors.affiliation: National Institute of Materials Physics, P.O. Box MG-7, Bucharest, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Feder
authors.affiliation: National Institute of Materials Physics, P.O. Box MG-7, Bucharest, Romania.
authors.forename: D
authors.initials: D
authors.lastname: Tarabasanu-Mihaila
authors.affiliation: National Institute of Materials Physics, P.O. Box MG-7, Bucharest, Romania.
authors.forename: V S
authors.initials: VS
authors.lastname: Teodorescu
authors.affiliation: National Institute of Materials Physics, P.O. Box MG-7, Bucharest, Romania.
authors.forename: A M
authors.initials: AM
authors.lastname: Vlaicu
authors.affiliation: National Institute of Materials Physics, P.O. Box MG-7, Bucharest, Romania.
authors.forename: L
authors.initials: L
authors.lastname: Diamandescu
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D001729:Bismuth / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012666:Semiconductors / Q000009:adverse effects*; D014025:Titanium / Q000633:toxicity*; D014961:X-Ray Diffraction
chemical_list: D017382:Reactive Oxygen Species; C550183:bismuth titanate; D009569:Nitric Oxide; D014025:Titanium; D001729:Bismuth
keywords: Bi(12)TiO(20); Bismuth oxide; Cytotoxicity; Oxidative stress; Sillenites; γ-Bi(2)O(3)