title: Cerebral hemorrhage increases plasma concentrations of noradrenalin and creatine kinase MB fraction with induction of cardiomyocyte damage in rats.
abstract: The incidence of cardiac damage is high during acute cerebral hemorrhage. The animal data on the relationship between cerebral apoplexy and cardiac damage are lacking. Thus, the aim of the study was to evaluate the effects of cerebral hemorrhage on plasma concentrations of monoamine transmitter noradrenalin (NA), creatine kinase muscle and brain (CK-MB) isoenzyme fraction, and cardiomyocyte changes in the rat model. In this study, 140 Wistar rats were randomly and equally divided into experimental and control groups, and collagenase was injected into the right caudate nucleus to induce cerebral hemorrhage in the experimental group. Plasma NA was analyzed using high-performance liquid chromatography with electrochemical detection and serum CK-MB was measured by enzyme reaction rate method. We found that both NA and CK-MB were elevated (p < 0.05) at 6 h after cerebral hematoma formation; the levels were 2.46 ± 0.05 μg/L and 3.51 ± 0.23 μkat/L, respectively. NA and CK-MB concentrations reached peak levels at 24 h which were found to be 3.52 ± 0.06 μg/L and 5.47 ± 0.49 μkat/L, respectively. Thereafter, NA and CK-MB concentrations decreased gradually. Plasma NA declined to the preoperative level (1.66 ± 0.03 μg/L) at 72 h, while CK-MB level (2.71 ± 0.17 μkat/L) was found to be still higher than its preoperative level. It was, therefore, concluded that plasma NA might be involved in the induction and development of cardiomyocytes damage during cerebral hemorrhage.
authors.affiliation: Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, 400042, China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Liang
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Jiang
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Xu
mesh_terms: D000818:Animals; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D002851:Chromatography, High Pressure Liquid; D017364:Collagenases / Q000633:toxicity; D052279:Creatine Kinase, MB Form / Q000097:blood*; D004195:Disease Models, Animal; D055664:Electrochemical Techniques; D008297:Male; D008854:Microscopy, Electron; D009206:Myocardium / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D009638:Norepinephrine / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D052279:Creatine Kinase, MB Form; D017364:Collagenases; D009638:Norepinephrine