title: Coating gigaporous polystyrene microspheres with cross-linked poly(vinyl alcohol) hydrogel as a rapid protein chromatography matrix.
abstract: Gigaporous polystyrene (PS) microspheres were hydrophilized by in situ polymerization to give a stable cross-linked poly(vinyl alcohol) (PVA) hydrogel coating, which can shield proteins from the hydrophobic PS surface underneath. The amination of microspheres (PS-NH2) was first carried out through acetylization, oximation and reduction, and then 4,4'-azobis (4-cyanovaleric acid) (ACV), a polymerization initiator, was covalently immobilized on PS-NH2 through amide bond formation, and the cross-linked poly(vinyl acetate) (PVAc) was prepared by radical polymerization at the surfaces of ACV-immobilized PS microspheres (PS-ACV). Finally, the cross-linked PVA hydrogel coated gigaporous PS microspheres (PS-PVA) was easily achieved through alcoholysis of PVAc. Results suggested that the PS microspheres were effectively coated with cross-linked PVA hydrogel, where the gigaporrous structure remained under optimal conditions. After hydrophilic modification (PS-PVA), the protein-resistant ability of microspheres was greatly improved. The hydroxyl-rich PS-PVA surface can be easily derivatized by classical chemical methods. Performance advantages of the PS-PVA column in flow experiment include good permeability, low backpressure, and mechanical stability. These results indicated that PS-PVA should be promising in rapid protein chromatography.
authors.affiliation: State Key Laboratory of Heavy Oil Processing, Center for Bioengineering and Biotechnology, China University of Petroleum (East China) , Qingdao 266580, P. R. China.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Qu
authors.forename: Guan-Sheng
authors.initials: GS
authors.lastname: Huan
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Chen
authors.forename: Wei-Qing
authors.initials: WQ
authors.lastname: Zhou
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
mesh_terms: D000327:Adsorption; D000818:Animals; D001391:Azo Compounds / Q000737:chemistry; D002417:Cattle; D002845:Chromatography / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000302:isolation & purification; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010539:Permeability; D056951:Photoelectron Spectroscopy; D011137:Polystyrenes / Q000737:chemistry*; D011143:Polyvinyl Chloride / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry; D016062:Porosity; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014631:Valerates / Q000737:chemistry
chemical_list: D001391:Azo Compounds; D003432:Cross-Linking Reagents; D011137:Polystyrenes; D011145:Polyvinyls; D012997:Solvents; D014631:Valerates; C039217:fluorescein isothiocyanate bovine serum albumin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C091250:4,4'-azobis(4-cyanovaleric acid); D012710:Serum Albumin, Bovine; C013215:polyvinyl acetate; D011143:Polyvinyl Chloride; D016650:Fluorescein-5-isothiocyanate