title: Gene silencing effect of SiRNA-magnetic modified with biodegradable copolymer nanoparticles on hTERT gene expression in lung cancer cell line.
abstract: BACKGROUND
  Telomerase is expressed in most of malignant cells, including lung cancer cells. The success of small interfering RNA (siRNA) in silencing of the telomerase catalytic subunit depends upon carriers ability to efficiently deliver therapeutic agent to cells with minimal toxicity and most biocompatibility. In this study, a potential carrier for efficient delivery was assessed by siRNA encapsulating into Iron MNPs modified with biodegradable polyester nanoparticles consisting of PLGA and PEG.
  
  
  RESULTS
  Data analysis shows that the self-assemble diblock copolymers were synthesized, and then the siRNA designed against hTERT catalytic subunit was encapsulated. Also, the rate of telomerase gene expression in equivalent with magnetic copolymers/siRNA was lower than that of free siRNA (P = 0.001).
  
  
  CONCLUSIONS
  In conclusion, regarding the enhancing of siRNA stability by magnetic copolymer, the expression of telomerase gene was significantly lower in the cells treated with siRNA-magnetic copolymers than those treated with free siRNA.
authors.affiliation: a Lung Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology , School of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Fekri Aval
authors.affiliation: c Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Akbarzadeh
authors.affiliation: b Department of Medical Biotechnology , School of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.|d Department of Clinical Biochemistry , Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Mohammad Rahmati
authors.initials: MR
authors.lastname: Yamchi
authors.affiliation: d Department of Clinical Biochemistry , Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Faraz
authors.initials: F
authors.lastname: Zarghami
authors.affiliation: b Department of Medical Biotechnology , School of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Kazem
authors.initials: K
authors.lastname: Nejati-Koshki
authors.affiliation: a Lung Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology , School of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.|d Department of Clinical Biochemistry , Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D004337:Drug Carriers / Q000737:chemistry*; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D052203:Ferrosoferric Oxide / Q000737:chemistry; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008168:Lung / Q000378:metabolism / Q000473:pathology; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection / Q000379:methods*
chemical_list: D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D034741:RNA, Small Interfering; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C509186:TERT protein, human; D019098:Telomerase; D052203:Ferrosoferric Oxide
keywords: SiRNA delivery; gene silencing; lung cancer; nanoparticles; telomerase