title: Enhanced lipid accumulation of green microalga Scenedesmus sp. by metal ions and EDTA addition.
abstract: Effects of Fe(3+) (0-0.12 g/L), Mg(2+) (0-0.73 g/L) and Ca(2+) (0-0.98 g/L) on the biomass and lipid accumulation of heterotrophic microalgae were investigated in dark environment. The biomass and lipid production exhibited an increasing trend with increasing the concentrations of metal ions. In cultures with 1.2 × 10(-3) g/L Fe(3+), 7.3 × 10(-3) g/L Mg(2+) and 9.8 × 10(-4) g/L Ca(2+), the maximum biomass, total lipid content and lipid productivity reached 3.49 g/L, 47.4% and 275.7 mg/L/d, respectively. More importantly, EDTA addition (1.0 × 10(-3) g/L) could enhance the solubility of metal ions (iron and calcium) and increase their availability by microalgae, which evidently promote the lipid accumulation. Compared with the control, the total lipid content and lipid productivity increased 28.2% and 29.7%, respectively. These show that appropriate concentrations of metal ions and EDTA in the culture medium were beneficial to lipid accumulation of heterotrophic Scenedesmus sp. cells.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: lbf@hit.edu.cn.
authors.forename: Bing-Feng
authors.initials: BF
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Fanying
authors.initials: F
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: rnq@hit.edu.cn.
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
mesh_terms: D002118:Calcium / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology*; D007477:Ions; D007501:Iron / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000096:biosynthesis; D008274:Magnesium / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D044444:Scenedesmus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
chemical_list: D007477:Ions; D008055:Lipids; D008670:Metals; D004492:Edetic Acid; D007501:Iron; D008274:Magnesium; D002118:Calcium
keywords: Algal cultivation; EDTA; Heterotrophic; Lipid accumulation; Metal ion