title: Characterisation of phosvitin phosphopeptides using MALDI-TOF mass spectrometry.
abstract: Putative phosphopeptides produced from enzyme hydrolysis of phosvitin were identified and characterised using MALDI-TOF/MS. Phosvitin was heat-pretreated and then hydrolysed using pepsin, thermolysin, and trypsin at their optimal pH and temperature conditions with or without partial dephosphorylation. Pepsin and thermolysin were not effective in producing phosphopeptides, but trypsin hydrolysis produced many peptides from phosvitin: 12 peptides, 10 of which were phosphopeptides, were identified from the trypsin hydrolysate. Twelve peptides were also identified from the trypsin hydrolysate of partially dephosphorylated phosvitin, but the phosphate groups remaining with the peptides were much smaller than those from the trypsin hydrolysate of intact phosvitin. This suggested that the phosphopeptides produced from the partially dephosphorylated phosvitin lost most of their phosphate groups during the dephosphorylation step. Therefore, partial dephosphorylation of phosvitin before trypsin hydrolysis may not be always recommendable in producing functional phosphopeptides if the phosphate groups play important roles for their functionalities.
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors.forename: Himali
authors.initials: H
authors.lastname: Samaraweera
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Department of Food and Nutrition, University of Wisconsin-Stout, Menomonie, WI 54751, USA.
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Biology, University of Wisconsin-Stout, Menomonie, WI 54751, USA.
authors.forename: Jenifer
authors.initials: J
authors.lastname: Grant
authors.affiliation: Applied Science Program, University of Wisconsin-Stout, Menomonie, WI 54751, USA.
authors.forename: Jordan
authors.initials: J
authors.lastname: Fouks
authors.affiliation: Functional Materials Research Group, Korea Food Research Institute, Sungnam, Gyeonggi-Do 463-746, South Korea.
authors.forename: Inwook
authors.initials: I
authors.lastname: Choi
authors.affiliation: Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do 449-728, South Korea. Electronic address: jwsuh@mju.ac.kr.
authors.forename: Joo Won
authors.initials: JW
authors.lastname: Suh
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA 50011, USA; Department of Animal Science and Technology, Sunchon National University, Sunchon 540-742, South Korea. Electronic address: duahn@iastate.edu.
authors.forename: Dong U
authors.initials: DU
authors.lastname: Ahn
mesh_terms: D010748:Phosphopeptides / Q000737:chemistry*; D010774:Phosvitin / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: D010748:Phosphopeptides; D010774:Phosvitin; D014357:Trypsin
keywords: Dephosphorylation; Enzymatic hydrolysis; MALDI-TOF/MS; Phosphopeptides; Phosvitin