title: The defective bone marrow-derived mesenchymal stem cells in patients with chronic immune thrombocytopenia.
abstract: Chronic immune thrombocytopenia (ITP) is characterized by autoimmune-mediated platelet destruction and impairment of thrombopoiesis. Mesenchymal stem cells (MSCs) are proposed to exhibit immune modulatory functions in self-tolerance maintenance. In this study, we aimed to characterize phenotypically and functionally bone marrow (BM)-derived MSCs from adult chronic ITP patients. Our results showed that BM-MSCs from patients with chronic ITP exhibited impaired proliferation, abnormal morphology and excessive apoptosis, and these defects could be ameliorated by modifying the culture environment. BM-MSCs from chronic ITP patients were shown to have similar immunophenotype and capacities to differentiate along adipogenic and osteogenic lineages as those from normal controls. However, the immune-inhibiting potential and the regulatory T cell-inducing ability of BM-MSCs from patients were defective compared to that of normal BM-MSCs. These findings suggest that the BM-MSCs were defective in chronic ITP patients. Whether the defective BM-MSCs are relevant to the pathogenesis of chronic ITP remains to be determined.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College , Tianjin , China .
authors.forename: Donglei
authors.initials: D
authors.lastname: Zhang
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Zeping
authors.initials: Z
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Chi
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Liu
authors.forename: Yueting
authors.initials: Y
authors.lastname: Huang
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Yang
authors.forename: Renchi
authors.initials: R
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology* / Q000473:pathology; D002454:Cell Differentiation / Q000276:immunology; D048708:Cell Growth Processes / Q000276:immunology; D018920:Coculture Techniques; D016207:Cytokines / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000473:pathology; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology* / Q000473:pathology; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D016207:Cytokines
keywords: Autoimmune; immunomodulation; mesenchymal stem cell; primary immune thrombocytopenia; regulatory T cells