title: Caffeic acid phenethyl ester protects against amphotericin B induced nephrotoxicity in rat model.
abstract: The present study was conducted to investigate whether caffeic acid phenethyl ester (CAPE), an active component of propolis extract, has a protective effect on amphotericin B induced nephrotoxicity in rat models. Male Wistar-Albino rats were randomly divided into four groups: (I) control group (n = 10), (II) CAPE group (n = 9) which received 10 μmol/kg CAPE intraperitoneally (i.p.), (III) amphotericin B group (n = 7) which received one dose of 50 mg/kg amphotericin B, and (IV) amphotericin B plus CAPE group (n = 7) which received 10 μmol/kg CAPE i.p. and one dose of 50 mg/kg amphotericin B. The left kidney was evaluated histopathologically for nephrotoxicity. Levels of malondialdehyde (MDA), nitric oxide (NO), enzyme activities including catalase (CAT), and superoxide dismutase (SOD) were measured in the right kidney. Histopathological damage was prominent in the amphotericin B group compared to controls, and the severity of damage was lowered by CAPE administration. The activity of SOD, MDA, and NO levels increased and catalase activity decreased in the amphotericin B group compared to the control group (P = 0.0001, P = 0.003, P = 0.0001, and P = 0.0001, resp.). Amphotericin B plus CAPE treatment caused a significant decrease in MDA, NO levels, and SOD activity (P = 0.04, P = 0.02, and P = 0.0001, resp.) and caused an increase in CAT activity compared with amphotericin B treatment alone (P = 0.005). CAPE treatment seems to be an effective adjuvant agent for the prevention of amphotericin B nephrotoxicity in rat models.
authors.affiliation: Department of Nephrology, School of Medicine, Internal Medicine, Süleyman Demirel University, East Campus, 32200 Isparta, Turkey.
authors.forename: Atila
authors.initials: A
authors.lastname: Altuntaş
authors.affiliation: Department of Medical Biology, School of Medicine, Mevlana University, 42030 Konya, Turkey.
authors.forename: H Ramazan
authors.initials: HR
authors.lastname: Yılmaz
authors.affiliation: Department of Dermatology, Isparta State Hospital, 32200 Isparta, Turkey.
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Altuntaş
authors.affiliation: Department of Biochemistry, School of Medicine, Süleyman Demirel University, 32200 Isparta, Turkey.
authors.forename: Efkan
authors.initials: E
authors.lastname: Uz
authors.affiliation: Department of Nephrology, School of Medicine, Internal Medicine, Süleyman Demirel University, East Campus, 32200 Isparta, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Demir
authors.affiliation: Department of Histology, School of Medicine, Süleyman Demirel University, 32200 Isparta, Turkey.
authors.forename: Alparslan
authors.initials: A
authors.lastname: Gökçimen
authors.affiliation: Department of Endocrinology and Metabolism, School of Medicine, Internal Medicine, Süleyman Demirel University, 32200 Isparta, Turkey.
authors.forename: Oğuzhan
authors.initials: O
authors.lastname: Aksu
authors.affiliation: Department of Histology, School of Medicine, Süleyman Demirel University, 32200 Isparta, Turkey.
authors.forename: Dilek Şenol
authors.initials: DŞ
authors.lastname: Bayram
authors.affiliation: Department of Nephrology, School of Medicine, Internal Medicine, Süleyman Demirel University, East Campus, 32200 Isparta, Turkey.
authors.forename: Mehmet Tuğrul
authors.initials: MT
authors.lastname: Sezer
mesh_terms: D000666:Amphotericin B / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects* / Q000494:pharmacology; D002109:Caffeic Acids / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D002109:Caffeic Acids; D009569:Nitric Oxide; D008315:Malondialdehyde; D000666:Amphotericin B; D002374:Catalase; D013482:Superoxide Dismutase; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol