title: In vivo magnetic resonance imaging at 11.7 Tesla visualized the effects of neonatal transection of infraorbital nerve upon primary and secondary trigeminal pathways in rats.
abstract: Using 11.7T ultra high-field T2-weighted MRI, the present study aimed to investigate pathological changes of primary and secondary trigeminal pathways following neonatal transection of infraorbital nerve in rats. The trigeminal pathways consist of spinal trigeminal tract, trigeminal sensory nuclear complex, medial lemniscus, ventromedial portion of external medullary lamina and ventral posterior nucleus of thalamus. By selecting optimum parameters of MRI such as repetition time, echo time, and slice orientation, this study visualized the trigeminal pathways in rats without any contrast agents. Pathological changes due to the nerve transection were found at 8 weeks of age as a marked reduction of the areas of the trigeminal pathways connecting from the injured nerve. In addition, T2-weighted MR images of the trigeminal nerve trunk and the spinal trigeminal tract suggest a communication of CSF through the trigeminal nerve between the inside and outside of the brain stem. These results support the utility of ultra high-field MRI system for noninvasive assessment of effects of trigeminal nerve injury upon the trigeminal pathways.
authors.affiliation: Division of Pathogenesis and Control of Oral Disease, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan; CREST, Japan Science and Technology Agency (JST), Saitama 332-0012, Japan. Electronic address: ooiyasuhiro03411@gmail.com.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ooi
authors.affiliation: CREST, Japan Science and Technology Agency (JST), Saitama 332-0012, Japan; High Performance Bioimaging Research Facility, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Chizuko
authors.initials: C
authors.lastname: Inui-Yamamoto
authors.affiliation: CREST, Japan Science and Technology Agency (JST), Saitama 332-0012, Japan; Department of Biomedical Engineering, National Cerebral & Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: CREST, Japan Science and Technology Agency (JST), Saitama 332-0012, Japan; Department of Biomedical Engineering, National Cerebral & Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nakadate
authors.affiliation: Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nagase
authors.affiliation: CREST, Japan Science and Technology Agency (JST), Saitama 332-0012, Japan; Division of Medical Devices for Diagnoses, Faculty of Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Akitoshi
authors.initials: A
authors.lastname: Seiyama
authors.affiliation: CREST, Japan Science and Technology Agency (JST), Saitama 332-0012, Japan; Biofunctional Imaging Laboratory, Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Yoshioka
authors.affiliation: CREST, Japan Science and Technology Agency (JST), Saitama 332-0012, Japan; Department of Biomedical Engineering, National Cerebral & Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Seki
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D008279:Magnetic Resonance Imaging; D008297:Male; D008442:Maxillary Nerve / Q000293:injuries*; D009434:Neural Pathways / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013788:Thalamus / Q000473:pathology; D012668:Trigeminal Ganglion / Q000473:pathology; D014276:Trigeminal Nerve / Q000473:pathology*; D014278:Trigeminal Nuclei / Q000473:pathology*
keywords: Critical period; Neonatal nerve transaction; Trigeminal pathways; Ultra high-field T2-weighted MR imaging