title: Pancreatic polypeptide inhibits somatostatin secretion.
abstract: Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of tissues that we tested, including pancreatic islets, duodenum, hippocampus, and hypothalamus. Its agonism by PP decreases somatostatin secretion from human islets. Mouse embryonic hippocampal (mHippo E18) cells expressed NPY4Rs and their activation by PP consistently decreased somatostatin secretion. Furthermore, central injection of PP in mice induced c-Fos immunoreactivity in somatostatin-containing cells in the hippocampus compared with PBS-injected mice. In sum, our results identify PP as a pivotal modulator of somatostatin secretion.
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, South Korea.
authors.forename: Wook
authors.initials: W
authors.lastname: Kim
authors.affiliation: Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Fiori
authors.affiliation: Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Yu-Kyong
authors.initials: YK
authors.lastname: Shin
authors.affiliation: The Mina and Everard Goodman Faculty of Life Sciences, The Leslie and Susan Gonda Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan 52900, Israel.
authors.forename: Eitan
authors.initials: E
authors.lastname: Okun
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, South Korea.
authors.forename: Jung Seok
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Laboratory of Behavioral Neuroscience, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors.forename: Peter R
authors.initials: PR
authors.lastname: Rapp
authors.affiliation: Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA. Electronic address: eganj@grc.nia.nih.gov.
authors.forename: Josephine M
authors.initials: JM
authors.lastname: Egan
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009928:Organ Specificity; D010191:Pancreatic Polypeptide / Q000494:pharmacology*; D017476:Receptors, Neuropeptide Y / Q000819:agonists / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism*
chemical_list: D017476:Receptors, Neuropeptide Y; D013004:Somatostatin; C109491:neuropeptide Y4 receptor; D010191:Pancreatic Polypeptide
keywords: NPY4 receptor; Pancreatic polypeptide; Somatostatin secretion