title: Inhibitory effects of the κ-casein macropeptide isolated from milk protein on the biofilm formation and virulence of Listeria monocytogenes.
abstract: We demonstrate the inhibitory effects of κ-casein macropeptide (CMP) on the biofilm formation and virulence of Listeria monocytogenes Scott A. The inhibition of biofilm formation by CMP was initially investigated by using the protocol applied for the 96-well microtiter plate assay. Low concentrations of CMP (0.1, 0.2, 0.3, 0.4, and 0.5 mg/mL) that were tested resulted in a profound inhibitory effect on biofilm formation at a concentration of 0.4 mg/mL. CMP also significantly repressed the transcription of inlA (encoding internalin A) that was responsible for the initial adhesion and invasion event, and prolonged the survival of Caenorhabditis elegans infected by L. monocytogenes. Two-dimensional gel electrophoresis showed that newly identified proteins in the presence of CMP were involved in the stress response and metabolic processes that have important roles in developing listerial biofilms. Our results suggest that CMP from milk protein would be capable of eliminating biofilm formation and virulence by L. monocytogenes in the food industry.
authors.affiliation: a BK21 Plus Graduate Program, Department of Animal Science, Institute of Rare Earth for Biological Application , Chonbuk National University , Jeonju , Korea.
authors.forename: Hyun Sun
authors.initials: HS
authors.lastname: Yun
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Kim
authors.forename: Mi Ri
authors.initials: MR
authors.lastname: Park
authors.forename: Sae Hun
authors.initials: SH
authors.lastname: Kim
authors.forename: Sejong
authors.initials: S
authors.lastname: Oh
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000382:microbiology; D002364:Caseins / Q000008:administration & dosage* / Q000737:chemistry; D002417:Cattle; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000472:pathogenicity; D008894:Milk Proteins / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D002364:Caseins; D008894:Milk Proteins
keywords: Caenorhabditis elegans; Listeria monocytogenes; biofilm formation; two-dimensional gel electrophoresis; κ-casein macropeptide