title: CD39/NTPDase-1 expression and activity in human umbilical vein endothelial cells are differentially regulated by leaf extracts from Rubus caesius and Rubus idaeus.
abstract: Many experimental studies have demonstrated the favorable biological activities of plants belonging to the genus Rubus, but little is known of the role of Rubus leaf extracts in the modulation of the surface membrane expression and activity of endothelial apyrase. The aim of this study was to assess the influence of 1-15 μg/ml Rubus extracts on CD39 expression and enzymatic activity, and on the activation (ICAM-1 expression) and viability of human umbilical vein endothelial cells (HUVEC). The polyphenolic contents and antioxidative capacities of extracts from dewberry (R. caesius L.) and raspberry (R. idaeus L.) leaves were also investigated. The techniques applied were flow cytometry (endothelial surface membrane expression of ICAM-1 and CD39), malachite green assay (CD39 activity), HPLC-DAD (quantitative analysis of polyphenolic extract), ABTS, DPPH and FRAP spectrometric assays (antioxidant capacity), and the MTT test (cell viability). Significantly increased CD39 expressions and significantly decreased ATPDase activities were found in the cells treated with 15 μg/ml of either extract compared to the results for the controls. Neither of the extracts affected cell proliferation, but both significantly augmented endothelial cell ICAM-1 expression. The overall antioxidant capacities of the examined extracts remained relatively high and corresponded well to the determined total polyphenol contents. Overall, the results indicate that under in vitro conditions dewberry and raspberry leaf extracts have unfavorable impact on endothelial cells.
authors.affiliation: Department of Haemostasis and Haemostatic Disorders, Medical University of Łódź, Łódź, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Dudzinska
authors.forename: Boguslawa
authors.initials: B
authors.lastname: Luzak
authors.forename: Magdalena
authors.initials: M
authors.lastname: Boncler
authors.forename: Joanna
authors.initials: J
authors.lastname: Rywaniak
authors.forename: Dorota
authors.initials: D
authors.lastname: Sosnowska
authors.forename: Anna
authors.initials: A
authors.lastname: Podsedek
authors.forename: Cezary
authors.initials: C
authors.lastname: Watala
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001081:Apyrase / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates / Q000037:antagonists & inhibitors / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D065856:Rubus / Q000737:chemistry* / Q000145:classification; D013045:Species Specificity
chemical_list: D015703:Antigens, CD; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D018799:Intercellular Adhesion Molecule-1; C004931:1,1-diphenyl-2-picrylhydrazyl; D001081:Apyrase; C067869:CD39 antigen