title: siRNA: novel therapeutics from functional genomics.
abstract: Long-term research on RNA interference led to an unfathomed understanding of the mechanism of siRNA-mediated silencing and finally siRNA has emerged as a powerful therapeutic tool. With siRNAs virtually every gene in the human genome contributing to a disease becomes amenable to regulation, thus opening unprecedented opportunities for drug discovery. siRNA has a well-established role as a tool for in vitro target screening and validation, besides these recent progresses of siRNA delivery in vivo, this has raised more expectations for siRNA-based drugs as the up-and-coming 'magic bullet'. Although a plethora of articles have been published with siRNA, the fundamentals of siRNA-mediated gene silencing and transforming the functional genomics to novel therapeutics are reviewed in this article with consideration to present hurdles as a new generation challenge.
authors.affiliation: a Department of Food Quality Assurance , College of Food Processing Technology and Bio Energy, Anand Agricultural University , Anand 388 110 , Gujarat , India.
authors.forename: B H
authors.initials: BH
authors.lastname: Joshi
authors.forename: K P
authors.initials: KP
authors.lastname: Pachchigar
mesh_terms: D018014:Gene Transfer Techniques; D005818:Genetic Engineering / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D023281:Genomics / Q000379:methods*; D058990:Molecular Targeted Therapy / Q000379:methods*; D034741:RNA, Small Interfering / Q000235:genetics* / Q000627:therapeutic use*
chemical_list: D034741:RNA, Small Interfering
keywords: RNA interference; clinical trial; high-throughput; in silico design; siRNA-based therapeutic