title: Detection of survivin 2α gene expression in thyroid nodules.
abstract: BACKGROUND
  Functional studies of the survivin splice variants have been performed almost exclusively in various types of cancer and produced remarkable advances in our understanding of cancer biology and cancer genetics.
  
  
  OBJECTIVE
  To observation the expression of survivin 2α in thyroid nodules and estimate its potential as a new molecular marker in thyroid nodules screening and malignant thyroid, as well.
  
  
  METHODS
  We detected the expression of a splice variant of survivin, survivin 2α, in thyroid nodules.
  
  
  METHODS
  Expression of survivin 2α mRNA was evaluated with specific primers by Hemi-Nested RT-PCR in 77 thyroid nodules including malignant and benign tumors, non-tumoral (goiter and thyroiditis) as well as surgical margin, non-neoplastic normal tissues adjacent to the malignant lesions.
  
  
  RESULTS
  Our data revealed for the first time the expression of survivin 2α in thyroid nodules. It was detected in 85.7% of non-neoplastic surgical margin tissues, 71.4% of non tumoral, 63.2% of tumoral samples. Also, the expression of survivin 2α in benign tumor samples (64.2%) is more than malignant groups (62.8%).
  
  
  CONCLUSIONS
  Survivin 2α expression is the highest in non-neoplastic surgical margin rather than other samples and the lowest expression was that of malignancy. According to the results, it can be concluded that survivin 2α protein may be has a vital protective effect throw survivin quenching due to the high expression in normal tissue compared with lesions.
authors.forename: Keyhaneh
authors.initials: K
authors.lastname: Kyani
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Babaei
authors.affiliation: Department of Animal Biology, School of Natural Sciences, University of Tabriz, Tabriz, Iran.
authors.forename: Mohammad Ali Hosseinpour
authors.initials: MA
authors.lastname: Feizi
authors.forename: Somayeh
authors.initials: S
authors.lastname: Vandghanooni
authors.forename: Vahid
authors.initials: V
authors.lastname: Montazeri
authors.forename: Monireh
authors.initials: M
authors.lastname: Halimi
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D000077022:Survivin; D016606:Thyroid Nodule / Q000378:metabolism*
chemical_list: C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D020033:Protein Isoforms; D000077022:Survivin