title: Lessons from yeast: the spindle pole body and the centrosome.
abstract: The yeast spindle pole body (SPB) is the functional equivalent of the centrosome. Most SPB components have been identified and their functions partly established. This involved a large variety of techniques which are described here, and the potential use of some of these in the centrosome field is highlighted. In particular, very useful structural information on the SPB was obtained from a reconstituted complex, the γ-tubulin complex, and also from a sub-particle, SPB cores, prepared by extraction of an enriched SPB preparation. The labelling of SPB proteins with GFP at the N or C termini, using GFP tags inserted into the genome, gave informative electron microscopy localization and fluorescence resonance energy transfer data. Examples are given of more precise functional data obtained by removing domains from one SPB protein, Spc110p, without affecting its essential function. Finally, a structural model for SPB duplication is described and the differences between SPB and centrosome duplication discussed.
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK jvk@mrc-lmb.cam.ac.uk.
authors.forename: John V
authors.initials: JV
authors.lastname: Kilmartin
mesh_terms: D002148:Calmodulin-Binding Proteins; D018385:Centrosome / Q000502:physiology*; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism; D010641:Phenotype; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D064048:Spindle Pole Bodies / Q000502:physiology* / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D015003:Yeasts
chemical_list: D002148:Calmodulin-Binding Proteins; D003598:Cytoskeletal Proteins; D009687:Nuclear Proteins; C073277:SPC110 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014404:Tubulin; D049452:Green Fluorescent Proteins
keywords: bodies; centrosomes; pole; spindle