title: Effects of different sizes of glass beads on the release of sporocysts from Eimeria tenella oocysts.
abstract: The oocyst wall is severed by means of mechanical injury or chemical agents. This study reports the percentage of in vitro sporocyst release following mechanical shaking in the presence of varying sizes of glass beads. Glass beads measured 0.5, 1, and 3 mm in diameter and were shaken with the oocysts for different times ranging from 5 sec to 5 min. Approximately 80% of sporocysts were released with 5 min of shaking in the presence of 3 mm glass beads, as well as 30 sec with 0.5 mm beads and 1 mm glass beads. The release of sporocysts of E. tenella was most efficient using 1 mm glass beads and treatment times of 30 sec to 1 min. Therefore, the use of 1 mm glass beads with 30 sec to 1 min of agitation is recommended in order to maximize sporocyst release and recovery and to improve the yield of viable sporozoites for use in biochemical, tissue culture, and immunological applications of coccidia.
authors.affiliation: Laboratory of Veterinary Parasitology, College of Veterinary Medicine and Bio-safety Research Center, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Myung-Jo
authors.initials: MJ
authors.lastname: You
mesh_terms: D016786:Eimeria tenella / Q000502:physiology*; D005898:Glass; D055595:Mechanical Phenomena; D008863:Microspheres; D033661:Oocysts / Q000502:physiology*; D010274:Parasitology / Q000379:methods*; D013312:Stress, Physiological; D013997:Time Factors
keywords: Eimeria tenella; glass bead; oocyst; sporocyst