title: MicroRNA-340 as a modulator of RAS-RAF-MAPK signaling in melanoma.
abstract: microRNA (miRNA)-dependent regulation of gene expression is increasingly linked to development and progression of melanoma. In this study we evaluated the functions of miR-340 in human melanoma cells. Here, we show that miR-340 inhibits the tumorigenic phenotype of melanoma cells. We also found that miR-340 regulates RAS-RAF-Mitogen Activated Protein Kinase (MAPK) signaling by modulating the expression of multiple components of this pathway. Given the importance of MAPK signaling in melanoma, these results provide further insight into the pathogenesis of melanoma.
authors.affiliation: Department of Dermatology, University of Wisconsin-Madison, School of Medicine and Public Health, 1300 University Avenue, Madison, WI 53706, United States; Molecular and Environmental Toxicology Center, University of Wisconsin-Madison, School of Medicine and Public Health, 1300 University Avenue, Madison, WI 53706, United States.
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Poenitzsch Strong
authors.affiliation: Department of Dermatology, University of Wisconsin-Madison, School of Medicine and Public Health, 1300 University Avenue, Madison, WI 53706, United States; Molecular and Environmental Toxicology Center, University of Wisconsin-Madison, School of Medicine and Public Health, 1300 University Avenue, Madison, WI 53706, United States; UW Carbone Cancer Center, University of Wisconsin-Madison, School of Medicine and Public Health, 600 Highland Avenue, Madison, WI 53792, United States.
authors.forename: Vijayasaradhi
authors.initials: V
authors.lastname: Setaluri
authors.affiliation: Department of Dermatology, University of Wisconsin-Madison, School of Medicine and Public Health, 1300 University Avenue, Madison, WI 53706, United States; Molecular and Environmental Toxicology Center, University of Wisconsin-Madison, School of Medicine and Public Health, 1300 University Avenue, Madison, WI 53706, United States; UW Carbone Cancer Center, University of Wisconsin-Madison, School of Medicine and Public Health, 600 Highland Avenue, Madison, WI 53792, United States. Electronic address: spiegelman@dermatology.wisc.edu.
authors.forename: Vladimir S
authors.initials: VS
authors.lastname: Spiegelman
mesh_terms: D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D020935:MAP Kinase Signaling System / Q000235:genetics*; D008545:Melanoma / Q000209:etiology / Q000235:genetics* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D015398:Signal Transduction / Q000235:genetics; D048490:raf Kinases / Q000378:metabolism*; D018631:ras Proteins / Q000378:metabolism*
chemical_list: C570046:MIRN340 microRNA, human; D035683:MicroRNAs; D048490:raf Kinases; D018631:ras Proteins
keywords: Invasion; MAPK; Melanoma; Proliferation; RAF; RAS; Tumor suppressor; miR-340; microRNA