title: The role of wogonin in controlling SOCS3 expression in neuronal cells.
abstract: The mechanism underlying the wogonin-mediated increase in the expression of suppressor of cytokine signaling 3 (SOCS3) is unclear. Promoter deletion assay results revealed that wogonin-induced SOCS3 expression is dependent on the AP-1 consensus sequences and two STAT responsive elements (TTACAAGAA and TTCCAGGAA) in the 5'-flanking region of the SOCS3 gene in SH-SY5Y cells. Wogonin-induced SOCS3 expression was blocked by inhibitors of PI3K, Akt, Raf, p38, JNK, MEK, and STAT3, respectively. However, JAK2 inhibitors did not inhibit wogonin-induced SOCS3 expression. These results indicate that SOCS3-inducing effect of wogonin is caused by the activation of PI3K-mediated MAPK signaling pathways (Akt, ERK1/2, p38, and JNK), and the subsequent activation of AP-1 consensus sequences and STAT responsive elements in SH-SY5Y cells.
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Science, Soonchunhyang University, Asan 336-745, Republic of Korea.
authors.forename: Kung-Woo
authors.initials: KW
authors.lastname: Nam
authors.affiliation: Herbal Quality Control Center, Korea Institute of Oriental Medicine, 488 Expo, Daejeon 305-811, Republic of Korea.
authors.forename: Sungwook
authors.initials: S
authors.lastname: Chae
authors.affiliation: Department of Microbiology, College of Medicine, Soonchunhyang University, Cheonan 330-721, Republic of Korea.
authors.forename: Ho-Yeon
authors.initials: HY
authors.lastname: Song
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Woongchon
authors.initials: W
authors.lastname: Mar
authors.affiliation: Department of Life Science and Biotechnology, College of Natural Science, Soonchunhyang University, Asan 336-745, Republic of Korea. Electronic address: mdhan@sch.ac.kr.
authors.forename: Man-Deuk
authors.initials: MD
authors.lastname: Han
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D044950:Flavanones / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics*
chemical_list: D044950:Flavanones; C106644:SOCS3 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; C085514:wogonin
keywords: AP1; Neuroinflammation; PI3K; SOCS3; STAT3; Wogonin