title: Evaluation of ALA-PDT of ovarian cancer in the Fisher 344 rat tumor model.
abstract: OBJECTIVE
  This study aimed to evaluate aminolevulinic acid-photodynamic therapy (ALA-PDT) in an experimental tumor model to expand the use of PDT in the treatment of ovarian cancer with peritoneal carcinosis.
  
  
  METHODS
  5-Aminolevulinic acid (ALA) (Photocure ASA, Norway) 60mg/kg was injected in the peritoneum cavity of 45 female rats Fisher with induced peritoneal metastases of ovarian cancer. ALA-PDT was performed 4h later with two different lasers: (1) laser diode (Diomed, Cambridge, UK), at 630nm, 100mW/cm(2), or (2) KTP laser (Laser Quantum, Stockport, UK), 532nm, 30mW/cm(2). The animals were divided into five groups: LASER ALONE group, CTRL group (no cancer), NO LASER group, 1 DOSE group (PDT during 1s) and 1.5 DOSE group (PDT during 1.5s). Biopsies were taken 24h after treatment. A semi-quantitative score called necrosis value (NV) was assigned to each sample that reflected the depth of the peritoneal necrosis.
  
  
  RESULTS
  In the first two groups, the peritoneum remained intact irrespective of the wavelength. In the 1 DOSE group, necrosis was observed for 532nm and 630nm. In the 1.5 DOSE group, necrosis was observed for 532nm (NV: 3.22±0.83) and 630nm (NV: 2.67±1.00) (p<0.05). The mesothelium strongly thinned out in the diffuse shape of the tumor.
  
  
  CONCLUSIONS
  Only ALA-PDT induces tumor necrosis with either 532nm and 630nm and should be considered an effective therapy for micrometastasis of ovarian cancer. This preliminary study deserves further experiments.
authors.affiliation: Department of Gynecology and Obstetrics, Lille University Hospital, Lille, France; INSERM (French National Institute of Health and Medical Research) IFR 114 & Lille University Hospital, Lille, France.
authors.forename: Manuel
authors.initials: M
authors.lastname: Ascencio
authors.affiliation: Department of Gynecology and Obstetrics, Lille University Hospital, Lille, France; INSERM (French National Institute of Health and Medical Research) IFR 114 & Lille University Hospital, Lille, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Delemer
authors.affiliation: Department of Anatomo-Pathology, Lille University Hospital, Lille, France.
authors.forename: M O
authors.initials: MO
authors.lastname: Farine
authors.affiliation: Department of Gynecology and Obstetrics, Lille University Hospital, Lille, France; INSERM (French National Institute of Health and Medical Research) IFR 114 & Lille University Hospital, Lille, France.
authors.forename: Eva
authors.initials: E
authors.lastname: Jouve
authors.affiliation: Department of Gynecology and Obstetrics, Lille University Hospital, Lille, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Collinet
authors.affiliation: INSERM (French National Institute of Health and Medical Research) IFR 114 & Lille University Hospital, Lille, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Mordon