title: Low-dose bisphenol A disrupts gonad development and steroidogenic genes expression in adult female rare minnow Gobiocypris rarus.
abstract: Bisphenol A (BPA), an estrogenic monomer, has attracted many researchers to study its adverse effects in animal reproduction, especially in fish. To explore the effects of low dose BPA on adult female rare minnow Gobiocypris rarus, we exposed the fish to BPA at nominal concentrations of 5, 15, and 50 μg L(-1) for 14 and 35 d. The 35-d BPA exposure at 50 μg L(-1) had obviously suppressive effects on oocyte development, and BPA at all the three concentrations in both exposure durations of 14 and 35 d had stimulative effects on hepatic vitellogenin (vtg) transcription. BPA at lower concentrations (5 and 15 μg L(-1)) exhibited stimulative effects on the expressions of ovarian steroidogenic genes while at higher concentration (50 μg L(-1)) displayed inhibitive effects. Analysis of ovarian steroidogenic genes 5'-flanking regions and mRNA expressions of their potential regulatory factors revealed that the BPA-mediated actions on steroidogenesis in G. rarus ovary probably involve estrogen receptor (Esr) and androgen receptor (Ar) signaling, nuclear receptor subfamily 5, group A, number 1 (Nr5a1) pathway, and epigenetic regulation.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Jiancao
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Qin
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. Electronic address: zzwang@nwsuaf.edu.cn.
authors.forename: Zaizhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D003530:Cyprinidae / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012733:Sex Differentiation / Q000187:drug effects; D013256:Steroids / Q000096:biosynthesis*; D014819:Vitellogenins / Q000235:genetics; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; D012333:RNA, Messenger; D013256:Steroids; D014819:Vitellogenins; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Gobiocypris rarus; Sex hormone receptor; Steroidogenic gene; Transcription factors