title: LPS-challenged TNFα production, prostaglandin secretion, and TNFα/TNFRs expression in the endometrium of domestic cats in estrus or diestrus, and in cats with pyometra or receiving medroxyprogesterone acetate.
abstract: Progesterone (P4) derivatives which are commonly used to block the cyclicity of domestic cats disturb the endocrine balance in the endometrium. The aims of this study were (i) to examine whether lipopolysaccharide (LPS) is responsible for enhancement of tumor necrosis factor-α (TNFα) secretion by the feline endometrial epithelial and stromal cells in vitro, (ii) to know whether immunolocalization of TNFα/TNFR1 and TNFR2 differs in cats at estrus or diestrus, receiving medroxyprogesterone acetate and suffering from pyometra, and (iii) to determine if TNFα-challenged prostaglandin secretion is stopped by prostaglandin synthases inhibitors. A total of 37 domestic adult cats in estrus or diestrus, receiving octane medroxyprogesterone or having clinical symptoms of pyometra, were enrolled in this study. The results obtained showed a distinct increase in LPS-challenged TNFα secretion in endometrial epithelial, but not stromal cells. TNFα augmented PG secretion was blocked by phospholipase A2 (PLA2) and cyclooxygeanase-2 (COX-2), but not by mitogen-activated protein kinase (MAPK) inhibitor. TNFα/TNFR1 and 2 protein expressions were limited mostly to the surface and glandular epithelium. TNFα/TNFRs protein was upregulated in the inflammatory uterus and hence may be involved in development of pathologic changes in the endometrial glands in cats receiving exogenous P4 as a hormonal contraceptive.
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Street, 10-748 Olsztyn, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Jursza
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Street, 10-748 Olsztyn, Poland.
authors.forename: Anna Z
authors.initials: AZ
authors.lastname: Szóstek
authors.affiliation: Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland.
authors.forename: Mariusz P
authors.initials: MP
authors.lastname: Kowalewski
authors.affiliation: Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland.
authors.forename: Alois
authors.initials: A
authors.lastname: Boos
authors.affiliation: Graduate School of Natural Science and Technology, Okayama University, Tsushima Naka, Okayama 700-8530, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okuda
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Street, 10-748 Olsztyn, Poland.
authors.forename: Marta J
authors.initials: MJ
authors.lastname: Siemieniuch
mesh_terms: D000818:Animals; D002415:Cats; D004031:Diestrus / Q000187:drug effects / Q000378:metabolism*; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism*; D004971:Estrus / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology*; D011453:Prostaglandins / Q000378:metabolism*; D055112:Pyometra / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D011453:Prostaglandins; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D017258:Medroxyprogesterone Acetate