title: Large-scale preparation of glycopeptides harboring the TF-antigen unit from royal jelly.
abstract: We have reported that new N-glycans carrying the TF-antigen occurred on a major royal jelly glycoprotein, and we have identified the glycosylation site to which the antigenic N-glycan is linked, but an appropriate procedure has not been established to prepare non-labeled immunoreactive glycopeptides in large amounts for functional analysis. In this study, we developed an effective method of preparing Asn-glycopeptide bearing TF-antigen.
authors.affiliation: a Department of Biofunctional Chemistry , Graduate School of Environmental and Life Science, Okayama University , Okayama , Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Maeda
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tanaka
authors.forename: Mariko
authors.initials: M
authors.lastname: Kimura
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry*; D006020:Glycopeptides / Q000378:metabolism*; D006031:Glycosylation; D000099219:Royal Jelly
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D005227:Fatty Acids; D006020:Glycopeptides; D000099219:Royal Jelly; C016467:Thomsen-Friedenreich antigen
keywords: TF-antigen unit; glycopeptide preparation; immunoreactive glycan; insect N-glycan; royal jelly