title: Cloning and starch degradation profile of maltotriose-producing amylases from Streptomyces species.
abstract: The end products from starch hydrolysis by amylases have important applications in various industries. Here, two amylases derived from two Streptomyces species that hydrolyze soluble starch from potato produced maltotriose as the primary maltooligosaccharide product. The genes, annotated as putative glycoside hydrolases, were cloned and expressed in Streptomyces lividans. These amylases displayed hydrolysis activity from pH 3 to 9.5 and were not affected by Ca(2+.) Optimal production of maltotriose was between 20 and 30 °C at pH 6.5. At the optimal temperature, both amylases produced maltotriose-rich end products rather than either maltose or maltotetraose.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan.
authors.forename: Norimasa
authors.initials: N
authors.lastname: Kashiwagi
authors.forename: Michiru
authors.initials: M
authors.lastname: Miyake
authors.forename: Shuichi
authors.initials: S
authors.lastname: Hirose
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sota
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000681:Amylases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013213:Starch / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D013696:Temperature; D014312:Trisaccharides / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D014312:Trisaccharides; C008317:maltotriose; D013213:Starch; D000681:Amylases