title: Immunoinformatics study on highly expressed Mycobacterium tuberculosis genes during infection.
abstract: The most important targets for vaccine development are the proteins that are highly expressed by the microorganisms during infection in-vivo. A number of Mycobacterium tuberculosis (Mtb) proteins are also reported to be expressed in-vivo at different phases of infection. In the present study, we analyzed multiple published databases of gene expression profiles of Mtb in-vivo at different phases of infection in animals and humans and selected 38 proteins that are highly expressed in the active, latent and reactivation phases. We predicted T- and B-cell epitopes from the selected proteins using HLAPred for T-cell epitope prediction and BCEPred combined with ABCPred for B-cell epitope prediction. For each selected proteins, regions containing both T- and B-cell epitopes were identified which might be considered as important candidates for vaccine design against tuberculosis.
authors.affiliation: Biotechnology Centre of Ho Chi Minh City, 176 Hai Ba Trung Street, Ho Chi Minh City, Viet Nam. Electronic address: lenacns83@yahoo.com.
authors.forename: Le Thuy
authors.initials: le T
authors.lastname: Nguyen Thi
authors.affiliation: PPSK, Health Campus, Universiti Sains Malaysia, Malaysia. Electronic address: sarmari14@gmail.com.
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Sarmiento
authors.affiliation: Finlay Institute, Cuba. Electronic address: rcalero@finlay.edu.cu.
authors.forename: Romel
authors.initials: R
authors.lastname: Calero
authors.affiliation: Universidad de Concepcion, Chile. Electronic address: frank.camacho@gmail.com.
authors.forename: Frank
authors.initials: F
authors.lastname: Camacho
authors.affiliation: Universidad de Concepcion, Chile. Electronic address: fatima8526@gmail.com.
authors.forename: Fatima
authors.initials: F
authors.lastname: Reyes
authors.affiliation: PPSK, Health Campus, Universiti Sains Malaysia, Malaysia. Electronic address: mmhossaink140@yahoo.com.
authors.forename: Md Murad
authors.initials: MM
authors.lastname: Hossain
authors.affiliation: Finlay Institute, Cuba. Electronic address: gsierra@finlay.edu.cu.
authors.forename: Gustavo Sierra
authors.initials: GS
authors.lastname: Gonzalez
authors.affiliation: PPSK, Health Campus, Universiti Sains Malaysia, Malaysia; INFORMM, Health Campus, Universiti Sains Malaysia, Malaysia. Electronic address: norazmimn@usm.my.
authors.forename: Mohd Nor
authors.initials: MN
authors.lastname: Norazmi
authors.affiliation: INFORMM, Health Campus, Universiti Sains Malaysia, Malaysia. Electronic address: ducmar13@gmail.com.
authors.forename: Armando
authors.initials: A
authors.lastname: Acosta
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D019295:Computational Biology; D030541:Databases, Genetic; D015195:Drug Design; D018985:Epitopes, B-Lymphocyte / Q000235:genetics*; D018984:Epitopes, T-Lymphocyte / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D016571:Neural Networks, Computer; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D032581:Tuberculosis Vaccines / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D032581:Tuberculosis Vaccines
keywords: Epitope prediction; Gene expression; Mycobacterium tuberculosis