title: Acellular blood vessels combined human hair follicle mesenchymal stem cells for engineering of functional arterial grafts.
abstract: Tissue-engineered vessels offer options for autologous vascular grafts in cardiovascular repair and regeneration. The experiments aimed to construct functional arterial grafts by combining human hair follicle mesenchymal stem cells (HF-MSCs) with acellular umbilical arteries. We isolated mesenchymal stem cells from human hair follicles. Under appropriate culture conditions, these cells displayed CD44, CD90 and CD105, and exhibited the potential for differentiation to adipocytes, osteoblasts and chondrocytes. Very promisingly, HF-MSCs expressed the vascular smooth muscle specific markers in the presence of transforming growth factor-β. We created acellular arterial scaffolds by digesting human umbilical arteries with trypsin and sodium dodecyl sulfate. These acellular arterial scaffolds retained major components of the extracellular matrix. The mechanical properties of these acellular arterial scaffolds were very similar to those of native blood vessels. We then seeded HF-MSCs into acellular arterial scaffolds and found that they still expressed vascular smooth muscle specific markers. The arterial grafts derived from HF-MSCs demonstrated vasoreactivity in response to humoral constrictors. We constructed arterial grafts that are very close to native blood vessels in their structures and physiological functions. These properties suggest that these arterial grafts could be used as small diameter arterial grafts for cardiovascular repair and regeneration.
authors.affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, 130021, Jilin, China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Gao
authors.forename: Feilin
authors.initials: F
authors.lastname: Liu
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhang
authors.forename: Xuejin
authors.initials: X
authors.lastname: Su
authors.forename: Jin Yu
authors.initials: JY
authors.lastname: Liu
authors.forename: Yulin
authors.initials: Y
authors.lastname: Li
mesh_terms: D001807:Blood Vessel Prosthesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D042783:Endothelial Cells / Q000502:physiology; D006025:Glycosaminoglycans / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000502:physiology; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014469:Umbilical Arteries / Q000502:physiology*
chemical_list: D006025:Glycosaminoglycans; D003094:Collagen