title: Vanadate influence on metabolism of sugar phosphates in fungus Phycomyces blakesleeanus.
abstract: The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in 31P NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (31P NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.
authors.affiliation: Institute for Multidisciplinary Research, Department of Life Sciences, University of Belgrade, Belgrade, Serbia.
authors.forename: Milan
authors.initials: M
authors.lastname: Žižić
authors.affiliation: University of Belgrade-Faculty of Biology, Department of Physiology and Biophysics, Belgrade, Serbia.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Živić
authors.affiliation: Institute for Multidisciplinary Research, Department of Life Sciences, University of Belgrade, Belgrade, Serbia.
authors.forename: Vuk
authors.initials: V
authors.lastname: Maksimović
authors.affiliation: Institute for Multidisciplinary Research, Department of Life Sciences, University of Belgrade, Belgrade, Serbia.
authors.forename: Marina
authors.initials: M
authors.lastname: Stanić
authors.affiliation: Institute for Multidisciplinary Research, Department of Life Sciences, University of Belgrade, Belgrade, Serbia.
authors.forename: Strahinja
authors.initials: S
authors.lastname: Križak
authors.affiliation: University of Belgrade-Faculty of Biology, Department of Physiology and Biophysics, Belgrade, Serbia.
authors.forename: Tijana Cvetić
authors.initials: TC
authors.lastname: Antić
authors.affiliation: Institute of General and Physical Chemistry, NMR Laboratory, Belgrade, Serbia.
authors.forename: Joanna
authors.initials: J
authors.lastname: Zakrzewska
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000502:physiology; D002384:Catalysis; D005658:Fungi / Q000378:metabolism*; D006019:Glycolysis / Q000502:physiology; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010800:Phycomyces / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism; D014638:Vanadates / Q000378:metabolism*
chemical_list: D011122:Polyphosphates; D013403:Sugar Phosphates; D014638:Vanadates; D000255:Adenosine Triphosphate; D014532:Uridine Diphosphate Glucose