title: Mimotope-based prediction of B-cell epitopes.
abstract: Mimotopes are peptides mimicking epitopes on the corresponding antigen. They can be obtained via panning the phage-displayed random peptide library against the corresponding monoclonal antibody or specific sera. Besides mimotopes however, the experimental results also include all kinds of unwanted sequences called "target-unrelated peptides," which often interfere with the subsequent experimental and computational analyses. Nevertheless, the prediction of B-cell epitopes based on the experimental result of phage display has shown to be a promising and reliable strategy with acceptable precision. In this chapter, we summarize mimotope-based prediction of B-cell epitopes under three conditions and focus on protocols and tips for retrieving, cleaning, and decoding the data from phage display technology.
authors.affiliation: Center of Bioinformatics (COBI), Key Laboratory for NeuroInformation of Ministry of Education, University of Electronic Science and Technology of China, Room 341, Yifu Building, Shahe Campus, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, China, hj@uestc.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Bifang
authors.initials: B
authors.lastname: He
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D016247:Information Storage and Retrieval / Q000379:methods; D019151:Peptide Library
chemical_list: D018985:Epitopes, B-Lymphocyte; D019151:Peptide Library