title: Theasaponin E₁ as an effective ingredient for anti-angiogenesis and anti-obesity effects.
abstract: Theasaponin E₁ (TSE₁) has been suggested to have higher biological activity than other saponins present in tea seed. Saponins have recently been considered as a potential chemotherapeutic agent for treating cancer. We examined the anti-angiogenic and anti-obesity properties of TSE₁ contributing to anti-cancer efficacy. Treating with a 10 μg/mL concentration of TSE₁ completely inhibited tube formation in human umbilical vein endothelial cells (HUVECs). TSE₁ showed toxicity toward cancer cells and inhibited in vivo growth of the tumor. The vascular endothelial growth factor (VEGF) receptor complex was suppressed, leading to the inhibition of protein kinase B (Akt) expression and down-regulation of nuclear factor-kappa B (NF-kB) activation. The differentiating 3T3-L₁ cells treated with TSE₁ had decreased lipid droplet formation measured by Oil Red O staining. Reduced weight was measured in mice fed with a TSE₁ plus high-fat diet. The results taken together, and particularly the NF-kB inhibition, suggest that TSE₁ may have multi-target action for treating cancer as a novel chemotherapeutic agent.
authors.affiliation: a Department of Biotechnology , Chonnam National University , Yeosu , Korea.
authors.forename: Jong-Deog
authors.initials: JD
authors.lastname: Kim
authors.forename: Narendra
authors.initials: N
authors.lastname: Chaudhary
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Seo
authors.forename: Min-Yong
authors.initials: MY
authors.lastname: Kim
authors.forename: Tai-Sun
authors.initials: TS
authors.lastname: Shin
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051379:Mice; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D020533:Angiogenesis Inhibitors; D019440:Anti-Obesity Agents; D000970:Antineoplastic Agents; D012333:RNA, Messenger; D012503:Saponins; C117120:theasaponin E1; D009828:Oleanolic Acid
keywords: NF-kB; VEGF; anti-angiogenesis; anti-obesity; theasaponin E1