title: Genome-wide translational changes induced by the prion [PSI+].
abstract: Prions are infectious proteins that can adopt a structural conformation that is then propagated among other molecules of the same protein. [PSI(+)] is an aggregated conformation of the translational release factor eRF3. [PSI(+)] modifies cellular fitness, inducing various phenotypes depending on genetic background. However, the genes displaying [PSI(+)]-controlled expression remain unknown. We used ribosome profiling in isogenic [PSI(+)] and [psi(-)] strains to identify the changes induced by [PSI(+)]. We found 100 genes with stop codon readthrough events and showed that many stress-response genes were repressed in the presence of [PSI(+)]. Surprisingly, [PSI(+)] was also found to affect reading frame selection independently of its effect on translation termination efficiency. These results indicate that [PSI(+)] has a broader impact than initially anticipated, providing explanations for the phenotypic differences between [psi(-)] and [PSI(+)] strains.
authors.affiliation: IGM, University of Paris-Sud, 91405 Orsay, France; CNRS, UMR 8621, 91405 Orsay, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Baudin-Baillieu
authors.affiliation: IGM, University of Paris-Sud, 91405 Orsay, France; CNRS, UMR 8621, 91405 Orsay, France.
authors.forename: Rachel
authors.initials: R
authors.lastname: Legendre
authors.affiliation: IGM, University of Paris-Sud, 91405 Orsay, France; CNRS, UMR 8621, 91405 Orsay, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Kuchly
authors.affiliation: IGM, University of Paris-Sud, 91405 Orsay, France; CNRS, UMR 8621, 91405 Orsay, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Hatin
authors.affiliation: IGM, University of Paris-Sud, 91405 Orsay, France; CNRS, UMR 8621, 91405 Orsay, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Demais
authors.affiliation: IGM, University of Paris-Sud, 91405 Orsay, France; CNRS, UMR 8621, 91405 Orsay, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Mestdagh
authors.affiliation: IGM, University of Paris-Sud, 91405 Orsay, France; CNRS, UMR 8621, 91405 Orsay, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gautheret
authors.affiliation: IGM, University of Paris-Sud, 91405 Orsay, France; CNRS, UMR 8621, 91405 Orsay, France. Electronic address: olivier.namy@igmors.u-psud.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Namy
mesh_terms: D004474:Ecthyma, Contagious; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D010443:Peptide Chain Termination, Translational; D011328:Prions / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D011328:Prions