title: Outside-out "sniffer-patch" clamp technique for in situ measures of neurotransmitter release.
abstract: The mechanism underlying neurotransmitter release is a critical research domain for the understanding of neuronal network function; however, few techniques are available for the direct detection and measurement of neurotransmitter release. To date, the sniffer-patch clamp technique is mainly used to investigate these mechanisms from individual cultured cells. In this study, we propose to adapt the sniffer-patch clamp technique to in situ detection of neurosecretion. Using outside-out patches from donor cells as specific biosensors plunged in acute cerebral slices, this technique allows for proper detection and quantification of neurotransmitter release at the level of the neuronal network.
authors.affiliation: Centre d'Investigation Clinique, Hospices Civils de Lyon, Hôpital des Charpennes, 27 rue Gabriel Péri, Villeurbanne, 69100, France, Emilie.chretien@chu-lyon.fr.
authors.forename: Emilie
authors.initials: E
authors.lastname: Muller-Chrétien
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D016466:CHO Cells; D003001:Cloning, Molecular / Q000379:methods; D003412:Cricetulus; D004867:Equipment Design; D005998:Glycine / Q000032:analysis / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D051379:Mice; D018377:Neurotransmitter Agents / Q000032:analysis / Q000378:metabolism*; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*; D018009:Receptors, Glycine / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: D018377:Neurotransmitter Agents; D018009:Receptors, Glycine; D005998:Glycine