title: Preparation and characterization of anionic pullulan thermoassociative nanoparticles for drug delivery.
abstract: Thermoassociative nanoparticles were obtained through the crosslinking reaction of periodate oxidized carboxymethyl pullulan with two difunctional Jeffamines: ED-600 and ED-2003. The nanoparticles were characterized through (1)H NMR spectra; their particle size, determined by dynamic light scattering (DLS) presented a bimodal distribution, with dimensions varying as a function of amount and type of crosslinking agent used; transmission electron microscopy photos confirmed the spherical shape of the nanoparticles and their dimensions determined by DLS. Their amphiphilic character was evidenced by retention of the dyes: hydrophobic (Rose Bengal), amphiphilic (Brilliant Blue) and hydrophilic (Vitamin B12). The thermosensitive properties, more pronounced for ED-2003 crosslinked nanoparticles, were evidenced through absorbance variation, fluorescence and DLS measurements as a temperature function. The nanoparticles retain important amounts of anionic (diclofenac: 40-80 mg/g), cationic (methylene blue: 70-125 mg/g) and hydrophobic (alpha-tocopherol: 220-350 mg/g) drugs. The in vitro characterization of the drug-polymer conjugates recommends the synthesized nanoparticles as supports for drug delivery.
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41a, 700487 Iasi, Romania. Electronic address: gmocanu@icmpp.ro.
authors.forename: G
authors.initials: G
authors.lastname: Mocanu
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41a, 700487 Iasi, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Nichifor
authors.affiliation: Normandie Université, France; Université de Rouen, Laboratoire Polymères Biopolymères Surfaces, F-76821 Mont Saint Aignan, France; CNRS UMR 6270 & FR3038, F-76821 Mont Saint Aignan, France.
authors.forename: L
authors.initials: L
authors.lastname: Picton
authors.affiliation: Normandie Université, France; Université de Rouen, Laboratoire Polymères Biopolymères Surfaces, F-76821 Mont Saint Aignan, France; CNRS UMR 6270 & FR3038, F-76821 Mont Saint Aignan, France.
authors.forename: E
authors.initials: E
authors.lastname: About-Jaudet
authors.affiliation: Normandie Université, France; Université de Rouen, Laboratoire Polymères Biopolymères Surfaces, F-76821 Mont Saint Aignan, France; CNRS UMR 6270 & FR3038, F-76821 Mont Saint Aignan, France.
authors.forename: D
authors.initials: D
authors.lastname: Le Cerf
mesh_terms: D000838:Anions / Q000737:chemistry; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage; D004396:Coloring Agents / Q000008:administration & dosage; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004008:Diclofenac / Q000008:administration & dosage; D065546:Drug Liberation; D005936:Glucans / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D013696:Temperature; D024502:alpha-Tocopherol / Q000008:administration & dosage
chemical_list: D000838:Anions; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004396:Coloring Agents; D003692:Delayed-Action Preparations; D005936:Glucans; D004008:Diclofenac; C009109:pullulan; D024502:alpha-Tocopherol
keywords: Amphiphilic; Drug release; Nanoparticles; Pullulan; Thermosensitive