title: Selection of DNA-encoded small molecule libraries against unmodified and non-immobilized protein targets.
abstract: The selection of DNA-encoded libraries against biological targets has become an important discovery method in chemical biology and drug discovery, but the requirement of modified and immobilized targets remains a significant disadvantage. With a terminal protection strategy and ligand-induced photo-crosslinking, we show that iterated selections of DNA-encoded libraries can be realized with unmodified and non-immobilized protein targets.
authors.affiliation: College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871 (China).
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Zitian
authors.initials: Z
authors.lastname: Chen
authors.forename: Yizhou
authors.initials: Y
authors.lastname: Li
authors.forename: Dawei
authors.initials: D
authors.lastname: Sun
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D015394:Molecular Structure; D011506:Proteins / Q000037:antagonists & inhibitors*; D054852:Small Molecule Libraries / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D008024:Ligands; D011506:Proteins; D054852:Small Molecule Libraries; D004247:DNA
keywords: DNA; DNA-encoded library; DNA-templated chemistry; drug discovery; selection