title: Femtogram detection of horseradish peroxidase by a common desktop scanner.
abstract: We report an image-based detection of horseradish peroxidase (HRP) by different color spaces. The results show excellent correlation between color saturation and absorbance (Pearson correlation coefficient; 0.9868) with respect to HRP. The present method can detect 185 and 46.45 fg/ml of HRP using o-phenylenediamine dihydrochloride and 3,3',5,5'-tetramethylbenzidine as chromogenic substrates respectively.
authors.affiliation: Department of Chemical and Systems Biology, CSIR-Institute of Genomics and Integrative Biology, Mall Road, New Delhi 110 007, India; Academy of Scientific and Innovative Research, Anushandhan Bhavan, 2, Rafi Marg, New Delhi 110001, India.
authors.forename: Shahila
authors.initials: S
authors.lastname: Parween
authors.affiliation: Department of Chemical and Systems Biology, CSIR-Institute of Genomics and Integrative Biology, Mall Road, New Delhi 110 007, India; Academy of Scientific and Innovative Research, Anushandhan Bhavan, 2, Rafi Marg, New Delhi 110001, India. Electronic address: pnahar@igib.res.in.
authors.forename: Pradip
authors.initials: P
authors.lastname: Nahar
mesh_terms: D001560:Benzidines / Q000378:metabolism; D002863:Chromogenic Compounds / Q000032:analysis / Q000378:metabolism; D003116:Color; D057075:Enzyme Assays / Q000295:instrumentation* / Q000379:methods*; D006735:Horseradish Peroxidase / Q000032:analysis* / Q000378:metabolism; D010655:Phenylenediamines / Q000378:metabolism
chemical_list: D001560:Benzidines; D002863:Chromogenic Compounds; D010655:Phenylenediamines; C021758:3,3',5,5'-tetramethylbenzidine; C034193:1,2-diaminobenzene; D006735:Horseradish Peroxidase
keywords: 3,3′,5,5′-Tetramethylbenzidine; Color space; Horseradish peroxidase; Image-based assay; Microtest plate; o-Phenylene diamine dihydrochloride