title: Characterization and genetic analyses of a carbazole-degrading gram-positive marine isolate, Janibacter sp. strain OC11.
abstract: Strain OC11 was isolated from seawater sampled at the coast of Chiba, Japan, in artificial seawater medium with carbazole (CAR) as the sole carbon source. Its 16S ribosomal RNA gene sequence suggested that strain OC11 belongs to the genus Janibacter. The CAR-degradation genes (car genes) of strain OC11 were PCR amplified, using degenerate primers designed based on the car gene sequences of other CAR-degrading bacteria. Complete nucleotide sequences encoding six complete open reading frames were determined, and the first known ferredoxin reductase gene (carAd) was found from a CAR-degrading bacterium isolated from the marine environment. An experiment using a mutant strain suggested that the car genes of strain OC11 are functional in CAR degradation. Southern hybridization indicated that strain OC11 had one car gene cluster in vivo. RT-PCR revealed that transcription of carOC11 constitutes an operon.
authors.affiliation: a Graduate School of Engineering and Science , Shibaura Institute of Technology , Saitama , Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Oba
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Rintaro
authors.initials: R
authors.lastname: Maeda
authors.forename: Toshio
authors.initials: T
authors.lastname: Omori
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fuse
mesh_terms: D000192:Actinomycetales / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D059001:Aquatic Organisms / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002227:Carbazoles / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002227:Carbazoles; D004269:DNA, Bacterial; C041514:carbazole
keywords: Janibacter; car gene cluster; carbazole