title: Requirement of dual stimulation by homologous recombinant IL-2 and recombinant IL-12 for the in vitro production of interferon gamma by canine peripheral blood mononuclear cells.
abstract: BACKGROUND
  Very few studies have been carried out so far aiming at modulating cellular immune responses in dogs. In this study, we evaluated the ability of recombinant canine IL-2 (rcaIL-2) and IL-12, in the form of a single-chain fusion protein (rsccaIL-12), to stimulate peripheral blood mononuclear cells (PBMC) of healthy mongrel dogs.
  
  
  RESULTS
  Recombinant canine IL-2 purified from Escherichia coli or present in the supernatant of COS-7 cells transfected with pcDNA3.1-caIL-2 (COS-7 caIL-2 supernatant) was able to induce proliferation of CTLL-2 cells, thus showing their functional activity. In addition, purified rcaIL-2 and COS-7 caIL-2 supernatant stimulated resting canine PBMC proliferation to a level higher than baseline level. Neither COS-7 sccaIL-12 supernatant nor COS-7 caIL-2 supernatant alone was able to induce significant production of interferon gamma by resting PBMC. However, COS-7 sccaIL-12 supernatant in combination with COS-7 caIL-2 supernatant induced production of IFN-γ by those cells.
  
  
  CONCLUSIONS
  The data shown herein suggest that the combination of canine recombinant IL-12 and IL-2 can be useful to promote cellular immune responses in dogs.
authors.forename: Andrea Mendes
authors.initials: AM
authors.lastname: Pereira
authors.forename: Cristiane Garboggini Melo
authors.initials: CG
authors.lastname: de Pinheiro
authors.forename: Lenita Ramires
authors.initials: LR
authors.lastname: Dos Santos
authors.forename: Naiara Carvalho
authors.initials: NC
authors.lastname: Teixeira
authors.forename: Yung-Fu
authors.initials: YF
authors.lastname: Chang
authors.forename: Lain Carlos
authors.initials: LC
authors.lastname: Pontes-de-Carvalho
authors.affiliation: Laboratório de Patologia e Bio-Intervenção, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Rua Waldemar Falcão, No, 121, Candeal, Salvador, Bahia, Brazil. ggileno@bahia.fiocruz.br.
authors.forename: Geraldo Gileno
authors.initials: GG
authors.lastname: de Sá Oliveira
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004285:Dogs; D004357:Drug Synergism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism; D018664:Interleukin-12 / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D061251:Primary Cell Culture; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*
chemical_list: D007376:Interleukin-2; D011993:Recombinant Fusion Proteins; D018664:Interleukin-12; D007371:Interferon-gamma