title: In vitro evaluation of the probiotic and functional potential of Lactobacillus strains isolated from fermented food and human intestine.
abstract: This study aims to evaluate the functional and probiotic characteristics of eight indigenous Lactobacillus strains in vitro. The selected lactobacilli include strains of Lactobacillus casei subsp. casei, Lactobacillus salivarius subsp. salicinius, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus delbrueckii subsp. lactis, Lactobacillus delbrueckii subsp. bulgaricus, and Lactobacillus rhamnosus. All strains tolerated both pH 2 for 3 h and 1% bile salt for 24 h. The strains CICC 23174 and CGMCC 1.557 were the most adhesive strains producing the highest quantity of EPS. Although a wide variation in the ability of the eight strains to deplete cholesterol and nitrite, antagonize pathogens, scavenge free radical, and stimulate innate immune response were observed, the strains CICC 23174 and CGMCC 1.557 showed the widest range of these useful traits. Taken together, the strains CICC 23174 and CGMCC 1.557 exhibited the best probiotic properties with the potential for use in the production of probiotic fermented foods.
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Ren
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China. Electronic address: lichang78@163.com.
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Academy of Animal Science and Veterinary Medicine in Jilin Province, Changchun 130062, PR China.
authors.forename: Ronglan
authors.initials: R
authors.lastname: Yin
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China.
authors.forename: Shouwen
authors.initials: S
authors.lastname: Du
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ye
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China.
authors.forename: Cunxia
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China.
authors.forename: Hongfeng
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China.
authors.forename: Maopeng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China.
authors.forename: Mingyao
authors.initials: M
authors.lastname: Tian
authors.affiliation: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, PR China. Electronic address: ningyij@hotmail.com.
authors.forename: Ningyi
authors.initials: N
authors.lastname: Jin
mesh_terms: D000143:Acids / Q000378:metabolism; D000898:Antibiosis; D001422:Bacterial Adhesion; D001647:Bile Acids and Salts / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D003902:Detergents / Q000633:toxicity; D005516:Food Microbiology; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007113:Immunity, Innate; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology*; D050296:Microbial Viability; D009573:Nitrites / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D019936:Probiotics / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D000143:Acids; D001647:Bile Acids and Salts; D003902:Detergents; D005609:Free Radicals; D009573:Nitrites; D011135:Polysaccharides, Bacterial; D002784:Cholesterol
keywords: Adhesion; Gastrointestinal tolerance; In vitro properties; Lactobacillus spp; Probiotics