title: Performance of the Abbott Real Time CT/NG assay in urines and cervico-vaginal samples from Senegal.
abstract: BACKGROUND
  Chlamydia trachomatis and Neisseria gonorrhoeae are the most common causes of sexually transmitted disease in Senegal and worldwide. Molecular techniques have become the standard for their detection, and due to the frequency of co-infections, these tests can detect both agents and can be used on urine samples, vaginal swabs, or endocervical samples. In developing countries, the use of these molecular techniques is very limited and there is a need for evaluations of these techniques to be done.
  
  
  METHODS
  A total of 181 samples were tested with the Abbott RealTime CT/NG assay and compared with the Roche Cobas Amplicor CT/NG assay. Specimens were collected from the key population of men having sex with men (urine, n = 60), female sex workers (genital swabs, n = 60) and from women visiting the laboratory for a gynecological checkup (urine, n = 60 and endocervical samples, n = 61).
  
  
  RESULTS
  The agreement between the two techniques was 98.90% with a Kappa coefficient of 0.98. A sensitivity of 93.3%, a specificity of 100%, a positive predictive value of 100%, and a negative predictive value of 93.3% were found for both Chlamydia trachomatis and Neisseria gonorrhoeae.
  
  
  CONCLUSIONS
  These results showed that both methods are similar and suitable for the detection of CT/NG in all types of samples examined in this study.
authors.affiliation: Université Cheikh Anta Diop, Dakar, Senegal. sbgueye@yahoo.fr.
authors.forename: Sokhna B
authors.initials: SB
authors.lastname: Gueye
authors.forename: Halimatou
authors.initials: H
authors.lastname: Diop-Ndiaye
authors.forename: Aliou
authors.initials: A
authors.lastname: Gningue
authors.forename: Ousseynou
authors.initials: O
authors.lastname: Ndiaye
authors.forename: Abdou S
authors.initials: AS
authors.lastname: Mbengue
authors.forename: Aïssatou
authors.initials: A
authors.lastname: Gaye-Diallo
authors.forename: Angelique
authors.initials: A
authors.lastname: Ndjioyi
authors.forename: Souleymane
authors.initials: S
authors.lastname: Mboup
authors.forename: Coumba
authors.initials: C
authors.lastname: Touré-Kane
mesh_terms: D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D060085:Coinfection; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D011237:Predictive Value of Tests; D012675:Senegal; D012680:Sensitivity and Specificity; D060386:Sex Workers; D012749:Sexually Transmitted Diseases / Q000382:microbiology*; D014556:Urine / Q000382:microbiology; D014621:Vagina / Q000382:microbiology