title: Diphenyl diselenide protects endothelial cells against oxidized low density lipoprotein-induced injury: Involvement of mitochondrial function.
abstract: Elevated levels of oxidized low density lipoprotein (oxLDL) are considered to be one of the major risk factors for atherosclerosis and cardiovascular morbidity. The early stages of atherosclerosis are initiated by the accumulation of oxLDL and the induction of toxic effects on endothelial cells, resulting in endothelial dysfunction. The aim of this study was to investigate how diphenyl diselenide (DD), an organoselenium compound, protect vascular endothelial cells against the toxic effects of oxLDL in vitro. Our data showed that the treatment of bovine endothelial aortic cells (BAEC) with DD (0.1-1 μM) for 24 h protected from oxLDL-induced reactive species (RS) production and reduced glutathione (GSH) depletion. Moreover, DD (1 μM) per se improved the maximal mitochondrial respiratory capacity and prevented oxLDL-induced mitochondrial damage. In addition, DD could prevent apoptosis induced by oxLDL in BAEC. Results from this study may provide insight into a possible molecular mechanism underlying DD suppression of oxLDL-mediated vascular endothelial dysfunction.
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88040900 Florianópolis, Santa Catarina, Brazil; Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.
authors.forename: Mariana Appel
authors.initials: MA
authors.lastname: Hort
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88040900 Florianópolis, Santa Catarina, Brazil.
authors.forename: Marcos Raniel
authors.initials: MR
authors.lastname: Straliotto
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88040900 Florianópolis, Santa Catarina, Brazil.
authors.forename: Jade
authors.initials: J
authors.lastname: de Oliveira
authors.affiliation: Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Laboratório de Bioquímica e Biologia Molecular do Câncer, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Nívea Dias
authors.initials: ND
authors.lastname: Amoêdo
authors.affiliation: Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: da Rocha
authors.affiliation: Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Laboratório de Bioquímica e Biologia Molecular do Câncer, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Antônio
authors.initials: A
authors.lastname: Galina
authors.affiliation: Departamento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Ribeiro-do-Valle
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88040900 Florianópolis, Santa Catarina, Brazil. Electronic address: andreza.bem@ufsc.br.
authors.forename: Andreza Fabro
authors.initials: AF
authors.lastname: de Bem
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D050197:Atherosclerosis / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D001555:Benzene Derivatives / Q000008:administration & dosage*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000633:toxicity; D008928:Mitochondria / Q000187:drug effects; D016566:Organoselenium Compounds / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000008:administration & dosage* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D001555:Benzene Derivatives; D008077:Lipoproteins, LDL; D016566:Organoselenium Compounds; D020011:Protective Agents; D017382:Reactive Oxygen Species; C099252:oxidized low density lipoprotein; C061132:diphenyldiselenide; D005978:Glutathione
keywords: Diphenyl diselenide; Endothelial cells; Mitochondria; Oxidative stress; Oxidized LDL