title: Use of an automated system for detection of canine serum antibodies against Ehrlichia canis glycoprotein 36.
abstract: Ehrlichia canis is the most common cause of monocytotropic ehrlichiosis in dogs around the world. The purpose of the present study was to validate a new automated fluorescence system (Accuplex4™ BioCD system; Antech Diagnostics, Lake Success, New York) to detect antibodies against the E. canis immunodominant glycoprotein 36 (gp36). Sera and blood samples (ethylenediamine tetra-acetic acid) were collected from mixed sex beagles ( n = 8) on days 0, 3, 7, 10, 14, 17, 21, 28, 42, 49, 56, 63, 70, 77, 84, and 98 after intravenous inoculation with culture-derived E. canis. Sera were assayed using the Accuplex4 BioCD system (Accuplex4), an E. canis indirect fluorescent antibody test (IFAT), and a commercially available kit. A complete blood cell count and a proprietary E. canis polymerase chain reaction (PCR) were performed on each blood sample. On the day thrombocytopenia was first detected for each dog, E. canis DNA was amplified from blood of all dogs. At those times, E. canis antibodies were detected in 7 of 8 dogs by the Accuplex4, 1 of 8 dogs by the commercial kit, and 4 of 8 dogs by IFAT. Ehrlichia canis DNA was amplified from blood before seroconversion in any antibody assay for 6 dogs. Antibodies against gp36 were detected by Accuplex4 within 3 days of PCR-positive test results and were detected up to 25 days sooner than the commercial kit. After starting doxycycline treatment, E. canis DNA was no longer amplified by PCR assay, but serum antibodies remained detectable by all assays.
authors.affiliation: Antech Diagnostics, Lake Success, NY (Moroff, Sokolchik, Woodring, Woodruff, Atkinson).|Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin).
authors.forename: Scott
authors.initials: S
authors.lastname: Moroff
authors.affiliation: Antech Diagnostics, Lake Success, NY (Moroff, Sokolchik, Woodring, Woodruff, Atkinson).|Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin).
authors.forename: Irene
authors.initials: I
authors.lastname: Sokolchik
authors.affiliation: Antech Diagnostics, Lake Success, NY (Moroff, Sokolchik, Woodring, Woodruff, Atkinson).|Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin).
authors.forename: Todd
authors.initials: T
authors.lastname: Woodring
authors.affiliation: Antech Diagnostics, Lake Success, NY (Moroff, Sokolchik, Woodring, Woodruff, Atkinson).|Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin).
authors.forename: Colby
authors.initials: C
authors.lastname: Woodruff
authors.affiliation: Antech Diagnostics, Lake Success, NY (Moroff, Sokolchik, Woodring, Woodruff, Atkinson).|Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin).
authors.forename: Brett
authors.initials: B
authors.lastname: Atkinson
authors.affiliation: Antech Diagnostics, Lake Success, NY (Moroff, Sokolchik, Woodring, Woodruff, Atkinson).|Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin).
authors.forename: Michael R
authors.initials: MR
authors.lastname: Lappin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000032:analysis; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004285:Dogs; D041061:Ehrlichia canis / Q000276:immunology*; D016873:Ehrlichiosis / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D005455:Fluorescent Antibody Technique / Q000379:methods / Q000662:veterinary*; D006023:Glycoproteins / Q000032:analysis
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D006023:Glycoproteins
keywords: Antigen; Ehrlichia canis; dogs; enzyme-linked immunosorbent assay; glycoprotein 36; indirect fluorescent antibody test; polymerase chain reaction