title: Autophagy-inducing peptides from mammalian VSV and fish VHSV rhabdoviral G glycoproteins (G) as models for the development of new therapeutic molecules.
abstract: It has not been elucidated whether or not autophagy is induced by rhabdoviral G glycoproteins (G) in vertebrate organisms for which rhabdovirus infection is lethal. Our work provides the first evidence that both mammalian (vesicular stomatitis virus, VSV) and fish (viral hemorrhagic septicemia virus, VHSV, and spring viremia carp virus, SVCV) rhabdoviral Gs induce an autophagic antiviral program in vertebrate cell lines. The transcriptomic profiles obtained from zebrafish genetically immunized with either Gsvcv or Gvhsv suggest that autophagy is induced shortly after immunization and therefore, it may be an important component of the strong antiviral immune responses elicited by these viral proteins. Pepscan mapping of autophagy-inducing linear determinants of Gvhsv and Gvsv showed that peptides located in their fusion domains induce autophagy. Altogether these results suggest that strategies aimed at modulating autophagy could be used for the prevention and treatment of rhabdoviral infections such as rabies, which causes thousands of human deaths every year.
authors.affiliation: IBMC; Miguel Hernández University; Elche, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: García-Valtanen
authors.affiliation: IBMC; Miguel Hernández University; Elche, Spain.
authors.forename: María Del Mar
authors.initials: Mdel M
authors.lastname: Ortega-Villaizán
authors.affiliation: IBMC; Miguel Hernández University; Elche, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Martínez-López
authors.affiliation: Labiofam; La Habana, Cuba.
authors.forename: Regla
authors.initials: R
authors.lastname: Medina-Gali
authors.affiliation: IBMC; Miguel Hernández University; Elche, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Pérez
authors.affiliation: Institute of Aquaculture; University of Stirling; Stirling, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Mackenzie
authors.affiliation: Instituto de Investigaciones Marinas (IIM); CSIC; Vigo, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Figueras
authors.affiliation: INIA-SIGT-Biotecnología; Madrid, Spain.
authors.forename: Julio M
authors.initials: JM
authors.lastname: Coll
authors.affiliation: IBMC; Miguel Hernández University; Elche, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Estepa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001343:Autophagy / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D029141:Novirhabdovirus / Q000302:isolation & purification*; D010455:Peptides / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D015027:Zebrafish
chemical_list: C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D010455:Peptides; D014759:Viral Envelope Proteins; D014764:Viral Proteins
keywords: LC3; SVCV; VHSV; VSV; antiviral; autophagy; immune response; microarrays; pepscan; rhabdovirus; viral glycoprotein; zebrafish