title: Short-term periodic consumption of multiprobiotic from childhood improves insulin sensitivity, prevents development of non-alcoholic fatty liver disease and adiposity in adult rats with glutamate-induced obesity.
abstract: BACKGROUND
  Today the impairment of metabolism and obesity are being extensively investigated due to the significant increase of the prevalence of these diseases. There is scientific evidence that probiotics are beneficial for human health. Thus, the aim of the study was to investigate the effect of multiprobiotic "Symbiter acidophilic concentrated" on obesity parameters in the rats under experimental obesity.
  
  
  METHODS
  The study was carried out on 60 newborn Wistar rats, divided into 3 groups, 20 animals in each (females - n = 10, males - n = 10): intact rats, monosodium glutamate (MSG-) and MSG + probiotic group. Rats of intact group were administered with saline (8 μl/g, subcutaneously (s.c.)). Newborns rats of MSG-group and MSG + probiotic group were injected with a solution of MSG (4.0 mg/g) s.c. at 2nd - 10th postnatal days. The MSG + probiotic group was treated with 140 mg/kg (1.4 × 10(10) CFU/kg) of multiprobiotic "Symbiter". MSG-group was treated with 2.5 ml/kg of water (per os) respectively. Administration was started at the age of 4 weeks just after wean and continued for 3 month intermittently alternating two-week course of introduction with two-week course of break.
  
  
  RESULTS
  Neonatal treatment with MSG caused a stunted growth in both MSG-groups, which manifested with significantly smaller naso-anal length compared to adult intact rats. There was no significant difference in weight between intact and MSG-groups on 120th day. The adiponectin level in the serum of rats with MSG-induced obesity decreased by 2.43 times (p = 0.001) in males and 1.75 (p = 0.020) in females. Concentration of leptin in adipose tissue were significantly higher by 45.9% (p = 0.019) and 61.2% (p = 0.009) respectively in males and females compared to intact rats. Our study has indicated that daily oral administration of multiprobiotic to neonatal MSG-treated rats by 2-week courses led to significant reduce of total body and VAT weight with subsequent improvement in insulin sensitivity and prevention of non-alcoholic fatty liver (NAFLD) development.
  
  
  CONCLUSIONS
  These results have shown that periodic treatment with multiprobiotic prevents the MSG-induced obesity and NAFLD development.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Savcheniuk
authors.affiliation: Bogomolets National Medical University, T, Shevchenko boulevard, 13, Kyiv 01601, Ukraine. nazariikobyliak@gmail.com.
authors.forename: Nazarii
authors.initials: N
authors.lastname: Kobyliak
authors.forename: Maryana
authors.initials: M
authors.lastname: Kondro
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Virchenko
authors.forename: Tetyana
authors.initials: T
authors.lastname: Falalyeyeva
authors.forename: Tetyana
authors.initials: T
authors.lastname: Beregova
mesh_terms: D052242:Adiponectin / Q000097:blood; D050154:Adiposity / Q000187:drug effects*; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects; D004334:Drug Administration Schedule; D005234:Fatty Liver / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D005260:Female; D007333:Insulin Resistance; D020738:Leptin / Q000097:blood; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease; D009765:Obesity / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism*; D019936:Probiotics / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012970:Sodium Glutamate
chemical_list: D052242:Adiponectin; D020738:Leptin; D012970:Sodium Glutamate