title: Detection of Neospora caninum-DNA in feces collected from dogs in Shenyang (China) and ITS1 phylogenetic analysis.
abstract: Neospora caninum is an intracellular protozoan that infects many domestic and wild animals. Dog is known as a definitive host of N. caninum and involved in transmitting infections to intermediate hosts by shedding oocysts. To investigate the epidemiology of dog neosporosis in China, 212 dog feces specimens in Shenyang were screened by nested-PCR using Nc5 primers and confirmed by N. caninum ITS1 PCR. The positive rate of N. caninum DNA was 34.90% (74/212). There were no significant correlations in prevalence of Neospora infections between different ages and genders. N. caninum DNA positive samples were further examined by PCR using Hammondia heydorni-specific primers. 37 out of 74 N. caninum DNA positive samples were also H. heydorni DNA positive. Only Nc5 primers positive and H. heydorni primers negative samples were used for ITS1 gene sequence analysis. Sequencing results from the 37 N. caninum positive samples revealed that ITS1 gene has 96-100% similarity with N. caninum sequences deposited in Genbank. Also, the presence of a new genotype indicated genetic polymorphism of N. caninum in infected dog feces in Shenyang of China.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China; Key Laboratory of Animal Clinical Diagnosis Technology, Ministry of Agriculture, Inter Mongolia Agricultural University, Huhhot 010018, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: He
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Du
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Guocai
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. Electronic address: xczhang@jlu.edu.cn.
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005260:Female; D008297:Male; D018688:Neospora / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer; D015800:Protozoan Proteins
keywords: Dog; ITS1; Nc5; Neospora caninum; Nested-PCR