title: Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas sobria strain Pt312.
abstract: The O-specific polysaccharide (OPS) obtained by mild-acid degradation of the lipopolysaccharide from Aeromonas sobria strain Pt312 was studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, NOESY, 1H-detected 1H,13C HSQC, and HMBC experiments. The sequence of the sugar residues was determined using 1H,1H NOESY and 1H,13C HMBC experiments. It was found that the OPS was built up of disaccharide repeating units composed of GlcpNAc and non-stoichiometrically O-acetylated Rhap residues, and had the structure.
authors.affiliation: Department of Genetics and Microbiology, M. Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. Electronic address: aturska@hektor.umcs.lublin.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Turska-Szewczuk
authors.affiliation: Department of Genetics and Microbiology, M. Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Hubert
authors.initials: H
authors.lastname: Pietras
authors.affiliation: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a/c, D-23845 Borstel, Germany.
authors.forename: Katarzyna A
authors.initials: KA
authors.lastname: Duda
authors.affiliation: Department of Fish Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Kozińska
authors.affiliation: Department of Fish Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Puławy, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Pękala
authors.affiliation: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a/c, D-23845 Borstel, Germany.
authors.forename: Otto
authors.initials: O
authors.lastname: Holst
mesh_terms: D000333:Aeromonas / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D002240:Carbohydrate Sequence; D007668:Kidney / Q000382:microbiology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D017686:Oncorhynchus mykiss / Q000382:microbiology
chemical_list: D019081:O Antigens
keywords: Aeromonas sobria; Fish pathogen; Lipopolysaccharide; NMR; O-Acetylation; O-specific polysaccharide