title: The PsbS protein plays important roles in photosystem II supercomplex remodeling under elevated light conditions.
abstract: Leaves from three different Arabidopsis lines with different expression levels of PsbS protein showed different levels of non-photochemical quenching. The PsbS deficient plant npq4 showed remarkable reduction of electron transport rate, while the other two lines with a moderate amount (wild type) or an overexpression of PsbS (L17) presented unchanged electron transport rates under the same range of high light intensities. Biochemical investigation revealed that the plant with the highest PsbS content (L17) sustained the highest level of stable PSII-LHCII supercomplex structure, and displayed the smallest fluorescence quenching in the thylakoid membranes, the most efficient linear electron transport and the smallest cyclic electron transport. Based on these observations, it is proposed that the remodeling of PSII-LHCII supercomplexes affected by PsbS plays important roles in regulating the energy balance in thylakoid membrane and in ensuring the sophisticated coordination between energy excitation and dissipation.
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Lianqing
authors.initials: L
authors.lastname: Dong
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Tu
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Sun
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address: yangch@ibcas.ac.cn.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Yang
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D004579:Electron Transport; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000235:genetics* / Q000378:metabolism; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; C489731:NPQ4 protein, Arabidopsis; D045332:Photosystem II Protein Complex
keywords: Arabidopsis; Non-photochemical quenching; PsbS; Thylakoid membrane remodeling