title: Wide-field multiplexed imaging of EGFR-targeted cancers using topical application of NIR SERS nanoprobes.
abstract: OBJECTIVE
  As the possibilities of molecular imaging in personalized medicine evolve rapidly, the optical advantages of extremely narrow and intense spectral bands makes surface-enhanced Raman scattering (SERS) an appealing candidate for multiplexed recognition of targeted biomarkers over other optical imaging modalities.
  
  
  METHODS
  In this proof-of-concept study, we report wide-field Raman detection of lung cancer using multimodal SERS nanoprobes specific to the EGF receptor family, both in vitro and in vivo.
  
  
  RESULTS
  For the first time, we demonstrate wide-field multiplexed Raman imaging for cancer detection in vivo after topical application of a 'cocktail' of SERS nanoprobes.
  
  
  CONCLUSIONS
  This advancement represents a key step towards sensitive wide-field Raman endoscopic imaging of multiple biomarkers for early and accurate diagnosis of EGF receptor-expressing tumors of different internal organs.
authors.affiliation: Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
authors.forename: Rupananda J
authors.initials: RJ
authors.lastname: Mallia
authors.forename: Patrick Z
authors.initials: PZ
authors.lastname: McVeigh
authors.forename: Carl J
authors.initials: CJ
authors.lastname: Fisher
authors.forename: Israel
authors.initials: I
authors.lastname: Veilleux
authors.forename: Brian C
authors.initials: BC
authors.lastname: Wilson
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000235:genetics / Q000473:pathology; D051379:Mice; D011859:Radiography; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: EGF receptor; anti-EGFR nanoprobe; cancer biomarker; gold nanoparticle; in vivo imaging; molecular imaging; multiplexed detection; ratiometric imaging; surface-enhanced Raman scattering; wide-field Raman