title: Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites.
abstract: This study deals with the nonaqueous capillary electrophoretic separation of dextromethorphan and its metabolites using a methanolic background electrolyte. The optimization of separation conditions was performed in terms of the resolution of dextromethorphan and dextrorphan and the effect of separation temperature, voltage, and the characteristics of the background electrolyte were studied. Complete separation of all analytes was achieved in 40 mM ammonium acetate dissolved in methanol. Hydrodynamic injection was performed at 3 kPa for 4 s. The separation voltage was 20 kV accompanied by a low electric current. The ultraviolet detection was performed at 214 nm, the temperature of the capillary was 25°C. These conditions enabled the separation of four analytes plus the internal standard within 9 min. Further, the developed method was validated in terms of linearity, sensitivity, and repeatability. Rat liver perfusate samples were subjected to the nonaqueous capillary electrophoretic method to illustrate its applicability.
authors.affiliation: Masaryk University, Faculty of Science, Department of Biochemistry, Kamenice, Czech Republic; Masaryk University, CEITEC - Central European Institute of Technology, Kamenice, Czech Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Pelcová
authors.forename: Monika
authors.initials: M
authors.lastname: Langmajerová
authors.forename: Eliška
authors.initials: E
authors.lastname: Cvingráfová
authors.forename: Jan
authors.initials: J
authors.lastname: Juřica
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Glatz
mesh_terms: D000818:Animals; D003915:Dextromethorphan / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D008968:Molecular Conformation; D051381:Rats
chemical_list: D004573:Electrolytes; D003915:Dextromethorphan; D000432:Methanol
keywords: Dextromethorphan; Dextrorphan; Methanol; Nonaqueous media