title: Regulation of the differentiation of osteoblasts and osteoclasts by a hot-water extract of adzuki beans (Vigna angularis).
abstract: Osteoporosis is a global public health problem thought to be caused by an imbalance in bone metabolism. We examined in this study the 40% ethanol fraction of HP-20 resin in combination with a hot-water adzuki extract (EtEx.40) for its effect on osteoblast and osteoclast differentiation. EtEx.40-treated murine preosteoblast MC3T3-E1 cells exhibited significantly elevated alkaline phosphatase activity and mineralization. EtEx.40 facilitated osteoblast differentiation by up-regulating such osteoblast differentiation-related molecules as runt-related transcription factor 2, distal-less homeobox 5, and osterix via p38 mitogen-activated protein kinase. EtEx.40 also suppressed the formation of large tartrate-resistant acid phosphatase-positive multinucleated cells in RAW264.7 cells that had been stimulated with the receptor activator of the nuclear factor κB ligand/macrophage colony-stimulating factor. EtEx.40 significantly inhibited NF-κB activation, thus reducing the expression of such downstream molecules as c-Fos and NFATc1. Our findings suggest that EtEx.40 could be used to maintain bone mass.
authors.affiliation: a Faculty of Agriculture , Kinki University , Nara , Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Itoh
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakamura
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Nakamichi
authors.forename: Masashi
authors.initials: M
authors.lastname: Ando
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Tsukamasa
authors.forename: Yukio
authors.initials: Y
authors.lastname: Furuichi
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D007887:Fabaceae / Q000737:chemistry*; D006358:Hot Temperature; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D053245:RANK Ligand / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects; D014867:Water / Q000737:chemistry*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D010936:Plant Extracts; D053245:RANK Ligand; D014867:Water; D016173:Macrophage Colony-Stimulating Factor; D048051:p38 Mitogen-Activated Protein Kinases
keywords: adzuki; differentiation; osteoblast; osteoclast; osteoporosis