title: Expression of P450 and nuclear receptors in normal and end-stage Chinese livers.
abstract: OBJECTIVE
  To investigate the expression of P450 enzyme genes by using end-stage liver disease samples and trimmed normal Chinese donor livers.
  
  
  METHODS
  The end-stage liver disease samples [n = 93, including hepatocellular carcinoma (HCC), peri-HCC tissue, hepatitis B virus cirrhosis, alcoholic cirrhosis, and severe cirrhosis] and trimmed normal Chinese donor livers (n = 35) from The Institute of Organ Transplantation in Beijing, China. Total RNA was extracted, purified, and subjected to real-time RT-PCR analysis.
  
  
  RESULTS
  For cytochrome P450 enzymes 1 (CYP1) family, the expression of CYP1A2 was decreased 90% in HCC, 80% in alcoholic cirrhosis, and 65% in severe cirrhosis. For CYP2 family, the expression of CAR was decreased 50% in HCC, but increased 50% in peri-HCC tissues. Similar decreases (about 50%) of CYP2B6, CYP2C9, CYP2C19, CYP2D6 and CYP2E1 were observed in HCC, as compared to peri-HCC tissues and normal livers. CYP2C19 were decreased in all end-stage liver diseases and CYP2E1 also decreased in alcoholic cirrhosis and severe cirrhosis. For CYP3 family, the expression of PXR was decreased 60% in HCC, together with decreases in CYP3A4, CYP3A5, and CYP3A7. In contrast, the expression of CYP3A7 was slightly increased in HBV cirrhosis. The expression of CYP4A11 was decreased 85% in HCC, 7% in alcoholic cirrhosis and severe liver cirrhosis, along with decreases in PPARα. The 93 end-stage livers had much higher inter-individual variations in gene expression than 35 normal livers.
  
  
  CONCLUSIONS
  The expression of CYP enzyme genes and corresponding nuclear receptors was generally decreased in end-stage liver diseases, and significant differences in gene expression were evident between peri-HCC and HCC.
authors.affiliation: Hong Chen, Zhong-Yang Shen, Wang Xu, Tie-Yan Fan, Jun Li, The Institute of Organ Transplantation, The General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Hong Chen, Zhong-Yang Shen, Wang Xu, Tie-Yan Fan, Jun Li, The Institute of Organ Transplantation, The General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors.forename: Zhong-Yang
authors.initials: ZY
authors.lastname: Shen
authors.affiliation: Hong Chen, Zhong-Yang Shen, Wang Xu, Tie-Yan Fan, Jun Li, The Institute of Organ Transplantation, The General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Xu
authors.affiliation: Hong Chen, Zhong-Yang Shen, Wang Xu, Tie-Yan Fan, Jun Li, The Institute of Organ Transplantation, The General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors.forename: Tie-Yan
authors.initials: TY
authors.lastname: Fan
authors.affiliation: Hong Chen, Zhong-Yang Shen, Wang Xu, Tie-Yan Fan, Jun Li, The Institute of Organ Transplantation, The General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Hong Chen, Zhong-Yang Shen, Wang Xu, Tie-Yan Fan, Jun Li, The Institute of Organ Transplantation, The General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors.forename: Yuan-Fu
authors.initials: YF
authors.lastname: Lu
authors.affiliation: Hong Chen, Zhong-Yang Shen, Wang Xu, Tie-Yan Fan, Jun Li, The Institute of Organ Transplantation, The General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors.forename: Ming-Liang
authors.initials: ML
authors.lastname: Cheng
authors.affiliation: Hong Chen, Zhong-Yang Shen, Wang Xu, Tie-Yan Fan, Jun Li, The Institute of Organ Transplantation, The General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D044466:Asian People / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000208:ethnology / Q000821:virology; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000235:genetics; D058625:End Stage Liver Disease / Q000175:diagnosis / Q000201:enzymology* / Q000208:ethnology / Q000235:genetics / Q000821:virology; D006509:Hepatitis B / Q000201:enzymology / Q000208:ethnology / Q000821:virology; D006801:Humans; D007527:Isoenzymes; D008099:Liver / Q000201:enzymology*; D008103:Liver Cirrhosis / Q000201:enzymology / Q000208:ethnology / Q000821:virology; D008104:Liver Cirrhosis, Alcoholic / Q000201:enzymology / Q000208:ethnology; D008113:Liver Neoplasms / Q000201:enzymology / Q000208:ethnology / Q000821:virology; D012333:RNA, Messenger / Q000032:analysis; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D003577:Cytochrome P-450 Enzyme System
keywords: Chinese donor livers; Cytochrome P450; End-stage livers; Hepatocellular carcinoma; Nuclear receptors; mRNA expression