title: Oral fluid as an alternative matrix to determine ethanol for forensic purposes.
abstract: The present work aimed to evaluate whether it is possible to use oral fluid to monitor alcohol in drivers. In a control experiment the subjects ingested beer with an alcoholic percentage of 4.7%, in an amount that furnished 0.5 g ethanol per kg of body weight. Volunteer's urine, oral fluid, and breath were collected at 10, 30, 60, and 90 min after alcohol intake. Urine and oral fluid were analyzed by gas chromatography with FID (Flame Ionization Detector); breath was analyzed by Alcotest 7410 (Dräger). The absorption profiles correlated well. The Pearson correlation value between samples of oral fluid and urine, and oral fluid and exhaled air, was close to 1, showing that oral fluid is a promising matrix to monitor drivers in traffic or involved in accidents.
authors.affiliation: Laboratório de Análises Toxicológicas Forenses, Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Brazil. Electronic address: laishbueno@usp.br.
authors.forename: Laís Helena Picolo
authors.initials: LHP
authors.lastname: Bueno
authors.affiliation: Departamento de Estomatologia, Saúde Coletiva e Odontologia Legal, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Brazil.
authors.forename: Ricardo Henrique Alves
authors.initials: RHA
authors.lastname: da Silva
authors.affiliation: Laboratório de Análises Toxicológicas Forenses, Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Brazil.
authors.forename: Aline Vieira
authors.initials: AV
authors.lastname: Azenha
authors.affiliation: Laboratório de Análises Toxicológicas Forenses, Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Brazil.
authors.forename: Mariane Cristine
authors.initials: MC
authors.lastname: de Souza Dias
authors.affiliation: Laboratório de Análises Toxicológicas Forenses, Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Brazil.
authors.forename: Bruno Spinosa
authors.initials: BS
authors.lastname: De Martinis
mesh_terms: D000428:Alcohol Drinking; D001334:Automobile Driving / Q000331:legislation & jurisprudence; D001944:Breath Tests; D002492:Central Nervous System Depressants / Q000032:analysis*; D000431:Ethanol / Q000032:analysis*; D005240:Feasibility Studies; D005260:Female; D005410:Flame Ionization; D053593:Forensic Toxicology; D006801:Humans; D057230:Limit of Detection; D008297:Male; D012463:Saliva / Q000737:chemistry*; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol
keywords: Alcohol; Breath; Gas chromatography; Oral fluid; Urine