title: [Interaction of FABP4 with plasma membrane proteins of endothelial cells].
abstract: BACKGROUND
  Fatty acid binding protein (FABP4) is an adipose tissue-secreted adipokine implicated in the regulation of the energetic metabolism and inflammation. High levels of circulating FABP4 have been described in people with obesity, atherogenic dyslipidemia, diabetes and metabolic syndrome. Recent studies have demonstrated that FABP4 could have a direct effect on peripheral tissues and, specifically, on vascular function. It is still unknown how the interaction between FABP4 and the endothelial cells is produced to prompt these effects on vascular function. The objective of this work is studying the interaction between FABP4 and the plasma membrane proteins of endothelial cells.
  
  
  METHODS
  HUVEC cells were incubated with and without FABP4 (100 ng/ml) for 5 minutes. Immunolocalization of FABP4 was studied by confocal microscopy. The results showed that FABP4 colocalizates with CD31, a membrane protein marker. A strategy which combines 6XHistidine-tag FABP4 (FABP4-His), incubations with or without FABP4-His (100 ng/ml), formaldehyde cross-linking, cellular membrane protein extraction and western blot, was designed to study the FABP4 interactions with membrane proteins of HUVECs.
  
  
  RESULTS
  The results showed different western blot profiles depending of the incubation with or without FABP4-His. The immunoblot revelead three covalent protein complexes of about 108, 77 and 33 kDa containing FAPB4 and its putative receptor.
  
  
  CONCLUSIONS
  The existence of a specific binding protein complex able to bind FABP4 to endothelial cells is supported by these results. The obtained results will permit us advance in the molecular knowledge of FABP4 effects as well as use this protein and its receptor as therapeutic target to prevent cardiovascular.
authors.affiliation: Unitat de Recerca en Lípids i Arteriosclerosi, Universitat Rovira i Virgili, Departamento de Medicina Interna, Hospital Universitari Sant Joan de Reus, IISPV, CIBERDEM, Reus, Tarragona, España.
authors.forename: Paula
authors.initials: P
authors.lastname: Saavedra
authors.affiliation: Unitat de Recerca en Lípids i Arteriosclerosi, Universitat Rovira i Virgili, Departamento de Medicina Interna, Hospital Universitari Sant Joan de Reus, IISPV, CIBERDEM, Reus, Tarragona, España.
authors.forename: Josefa
authors.initials: J
authors.lastname: Girona
authors.affiliation: Unitat de Recerca en Lípids i Arteriosclerosi, Universitat Rovira i Virgili, Departamento de Medicina Interna, Hospital Universitari Sant Joan de Reus, IISPV, CIBERDEM, Reus, Tarragona, España.
authors.forename: Gemma
authors.initials: G
authors.lastname: Aragonès
authors.affiliation: Unitat de Recerca en Lípids i Arteriosclerosi, Universitat Rovira i Virgili, Departamento de Medicina Interna, Hospital Universitari Sant Joan de Reus, IISPV, CIBERDEM, Reus, Tarragona, España.
authors.forename: Anna
authors.initials: A
authors.lastname: Cabré
authors.affiliation: Unitat de Recerca en Lípids i Arteriosclerosi, Universitat Rovira i Virgili, Departamento de Medicina Interna, Hospital Universitari Sant Joan de Reus, IISPV, CIBERDEM, Reus, Tarragona, España.
authors.forename: Sandra
authors.initials: S
authors.lastname: Guaita
authors.affiliation: Unitat de Recerca en Lípids i Arteriosclerosi, Universitat Rovira i Virgili, Departamento de Medicina Interna, Hospital Universitari Sant Joan de Reus, IISPV, CIBERDEM, Reus, Tarragona, España.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Heras
authors.affiliation: Unitat de Recerca en Lípids i Arteriosclerosi, Universitat Rovira i Virgili, Departamento de Medicina Interna, Hospital Universitari Sant Joan de Reus, IISPV, CIBERDEM, Reus, Tarragona, España. Electronic address: luis.masana@urv.cat.
authors.forename: Lluís
authors.initials: L
authors.lastname: Masana
mesh_terms: D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D008565:Membrane Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: Células endoteliales; Endothelial cells; FABP4; Membrana celular; Plasma membrane; Receptor