title: Colorimetric estimation of human glucose level using γ-Fe₂O₃ nanoparticles: an easily recoverable effective mimic peroxidase.
abstract: This article reports simple, green and efficient synthesis of γ-Fe₂O₃ nanoparticles (NPs) (maghemite) through single-source precursor approach for colorimetric estimation of human glucose level. The γ-Fe₂O₃ NPs, having cubic morphology with an average particle size of 30 nm, exhibited effective peroxidase-like activity through the catalytic oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ producing a blue-colored solution. On the basis of this colored-reaction, we have developed a simple, cheap, highly sensitive and selective colorimetric method for estimation of glucose using γ-Fe₂O₃/TMB/glucose-glucose oxidase (GOx) system in the linear range from 1 to 80 μM with detection limit of 0.21 μM. The proposed glucose sensor displays faster response, good stability, reproducibility and anti-interference ability. Based on this simple reaction process, human blood and urine glucose level can be monitored conveniently.
authors.affiliation: Department of Chemistry, Prasanta Chandra Mahalanobis Mahavidyalaya, 111/3 B.T.Road, Kolkata 700108, India.
authors.forename: Kamala
authors.initials: K
authors.lastname: Mitra
authors.affiliation: Department of Chemistry, Indian Institute of Engineering Science and Technology, Shibpur, Howrah 711 103, West Bengal, India.
authors.forename: Abhisek Brata
authors.initials: AB
authors.lastname: Ghosh
authors.affiliation: Department of Chemistry, Indian Institute of Engineering Science and Technology, Shibpur, Howrah 711 103, West Bengal, India.
authors.forename: Arpita
authors.initials: A
authors.lastname: Sarkar
authors.affiliation: Department of Chemistry, Indian Institute of Engineering Science and Technology, Shibpur, Howrah 711 103, West Bengal, India.
authors.forename: Namrata
authors.initials: N
authors.lastname: Saha
authors.affiliation: Department of Chemistry, Indian Institute of Engineering Science and Technology, Shibpur, Howrah 711 103, West Bengal, India. Electronic address: amtdtta@rediffmail.com.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Dutta
mesh_terms: D001560:Benzidines / Q000737:chemistry; D032701:Biomimetics; D015374:Biosensing Techniques / Q000379:methods; D001786:Blood Glucose / Q000032:analysis; D003124:Colorimetry / Q000379:methods*; D005290:Ferric Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D006029:Glycosuria / Q000175:diagnosis; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D001560:Benzidines; D001786:Blood Glucose; D005290:Ferric Compounds; C000499:ferric oxide; C021758:3,3',5,5'-tetramethylbenzidine; D006861:Hydrogen Peroxide; D010544:Peroxidases; D005947:Glucose
keywords: Colorimetric glucose determination; Human blood-urine; Hydrogen peroxide; Peroxidase-like activity; γ-Fe(2)O(3) nanoparticles