title: Proteomic analysis reveals differences in tolerance to acid rain in two broad-leaf tree species, Liquidambar formosana and Schima superba.
abstract: Acid rain (AR) is a serious environmental issue inducing harmful impacts on plant growth and development. It has been reported that Liquidambar formosana, considered as an AR-sensitive tree species, was largely injured by AR, compared with Schima superba, an AR-tolerant tree species. To clarify the different responses of these two species to AR, a comparative proteomic analysis was conducted in this study. More than 1000 protein spots were reproducibly detected on two-dimensional electrophoresis gels. Among them, 74 protein spots from L. formosana gels and 34 protein spots from S. superba gels showed significant changes in their abundances under AR stress. In both L. formosana and S. superba, the majority proteins with more than 2 fold changes were involved in photosynthesis and energy production, followed by material metabolism, stress and defense, transcription, post-translational and modification, and signal transduction. In contrast with L. formosana, no hormone response-related protein was found in S. superba. Moreover, the changes of proteins involved in photosynthesis, starch synthesis, and translation were distinctly different between L. formosana and S. superba. Protein expression analysis of three proteins (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, ascorbate peroxidase and glutathione-S-transferase) by Western blot was well correlated with the results of proteomics. In conclusion, our study provides new insights into AR stress responses in woody plants and clarifies the differences in strategies to cope with AR between L. formosana and S. superba.
authors.affiliation: Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Hu
authors.affiliation: Institute of Urban and Environment, Chinese Academy of Sciences, Xiamen, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Biology, Huaiyin Normal University, Huaian, Jiangsu, P.R. China; Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China.
authors.forename: Ting-Wu
authors.initials: TW
authors.lastname: Liu
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong, SAR, China.
authors.forename: Annie
authors.initials: A
authors.lastname: Chalifour
authors.affiliation: Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Shen
authors.affiliation: Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Wang
authors.affiliation: Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China.
authors.forename: Hai-Lei
authors.initials: HL
authors.lastname: Zheng
mesh_terms: D015258:Acid Rain; D000222:Adaptation, Physiological; D031317:Liquidambar / Q000378:metabolism*; D010788:Photosynthesis; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D013312:Stress, Physiological; D028242:Theaceae / Q000378:metabolism*
chemical_list: D015258:Acid Rain; D010940:Plant Proteins; D020543:Proteome