title: A region corresponding to second aspartate-rich motif in tryptophan isoprenylating enzyme, ComQ, serves as a substrate-binding site.
abstract: Posttranslational isoprenylation of a tryptophan residue identified from Bacillus quorum sensing pheromone, ComX pheromone, is unique and essential for the bioactivity. A modifying enzyme, ComQ, forms ComX pheromone from the ComX precursor and isoprenyl pyrophosphate and exhibits moderate similarity to isoprenyl pyrophosphate synthases. We investigated non-conserved region in ComQ, corresponding to isopentenyl pyrophosphate binding region of the synthases, using in vitro cell-free isoprenylation. These results suggested that the only conserved aspartic acid residue in the region of ComQ is critical for enzyme activity and responsible for ComX binding. Our findings should contribute to basic understanding of the mechanism of tryptophan isoprenylation.
authors.affiliation: a Graduate School of Bioscience and Biotechnology, Chubu University , Kasugai , Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okada
authors.forename: Ayako
authors.initials: A
authors.lastname: Ishihara
authors.forename: Risa
authors.initials: R
authors.lastname: Yamasaki
authors.forename: Fumitada
authors.initials: F
authors.lastname: Tsuji
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Hayashi
authors.forename: Syohei
authors.initials: S
authors.lastname: Usami
authors.forename: Youji
authors.initials: Y
authors.lastname: Sakagami
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001224:Aspartic Acid; D001412:Bacillus subtilis; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D054876:Prenylation; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C070739:comQ protein, Bacillus subtilis; D001224:Aspartic Acid; D014364:Tryptophan
keywords: Bacillus subtilis; isoprenylation; posttranslational modification; quorum sensing; tryptophan