title: Choline and acetylcholine detection based on peroxidase-like activity and protein antifouling property of platinum nanoparticles in bovine serum albumin scaffold.
abstract: Platinum nanoparticles (PtNPs) in the scaffold of bovine serum albumin (BSA) through biomineralization are found to possess excellent peroxidase-like activity that can catalyze N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt (TOPS) coupled with 4-amino-antipyrine (4-AAP) by the action of hydrogen peroxide to give an obvious purple product. Based on this phenomenon, acetylcholinesterase (AChE) and choline oxidase (ChOx) are used to catalyze ACh and choline to form the active product H2O2 and the as-produced H2O2 is detected optically. Owning to the protection effect of the protein shell, BSA-PtNPs turn out to be very stable and preserve the catalytic activity in the presence of protein and even in the real plasma samples. This protein antifouling property makes the BSA-PtNPs suitable for a wide range of applications in sensors for biological samples. Choline in infant formula and ACh in plasma have been successfully detected.
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Shao-Bin
authors.initials: SB
authors.lastname: He
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Department of Pharmacy, Fujian Provincial Hospital, Fuzhou 350001, China.
authors.forename: Gang-Wei
authors.initials: GW
authors.lastname: Wu
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Hao-Hua
authors.initials: HH
authors.lastname: Deng
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Ai-Lin
authors.initials: AL
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Xia
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China. Electronic address: chenandhu@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000109:Acetylcholine / Q000032:analysis* / Q000097:blood; D000110:Acetylcholinesterase; D000429:Alcohol Oxidoreductases; D000675:Ampyrone; D000814:Aniline Compounds; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D002794:Choline / Q000032:analysis*; D004800:Enzymes, Immobilized; D006801:Humans; D006861:Hydrogen Peroxide; D007223:Infant; D041943:Infant Formula / Q000737:chemistry; D053768:Metal Nanoparticles; D010084:Oxidation-Reduction; D009195:Peroxidase; D010984:Platinum; D012710:Serum Albumin, Bovine
chemical_list: D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D000675:Ampyrone; D012710:Serum Albumin, Bovine; D010984:Platinum; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D009195:Peroxidase; D000110:Acetylcholinesterase; D002794:Choline; D000109:Acetylcholine
keywords: Acetylcholine; BSA–PtNPs; Choline; Peroxidase-like activity; Protein antifouling property