title: Polymorphisms of glutathione peroxidase 1 (GPX1 Pro198Leu) and catalase (CAT C-262T) in women with spontaneous abortion.
abstract: About 10%-15% of conceptions are lost spontaneously prior to 20 weeks. Apart from the clinical problems, genetic variations have also been proposed as a susceptibility factor to miscarriage. Glutathione peroxidase 1 (GPX1) and catalase (CAT) encode two antioxidant enzymes that detoxify H2O2 and protect the cells from oxidative damage. A functional polymorphism at codon 198 of the GPX1 gene causes a C/T substitution in exon 2, which encodes for either proline or leucine (Pro198Leu). The CAT gene has a polymorphic site in the promoter region at position -262 (C-262T) which alters the expression and enzyme blood levels, leading to some pathological clinical conditions. In this study, we evaluated the association of these two polymorphisms with the risk of spontaneous abortion. Genomic DNA from 105 cases with spontaneous abortion and 90 healthy women were genotyped using allele-specific PCR (AS-PCR) and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The genetic distributions for GPX1 did not differ significantly between cases and controls (p = 0.680). However, C-262T polymorphism was significantly associated with the risk of the disease (OR, 5.50; 95% CI, 1.43-21.09; p = 0.012). In conclusion, this study indicates that CAT -262T/T genotype confers less susceptibility to spontaneous abortion, while GPX1 Pro198Leu polymorphism may not be correlated with the disease.
authors.affiliation: Department of Biology, Faculty of Sciences, University of Guilan , Rasht , Iran .
authors.forename: Eliza Eskafi
authors.initials: EE
authors.lastname: Sabet
authors.forename: Zivar
authors.initials: Z
authors.lastname: Salehi
authors.forename: Siamak
authors.initials: S
authors.lastname: Khodayari
authors.forename: Samin Sabouhi
authors.initials: SS
authors.lastname: Zarafshan
authors.forename: Ziba
authors.initials: Z
authors.lastname: Zahiri
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002374:Catalase / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007930:Leucine / Q000235:genetics*; D011110:Polymorphism, Genetic; D011392:Proline / Q000235:genetics*; D055815:Young Adult; D000094765:Glutathione Peroxidase GPX1
chemical_list: D017931:DNA Primers; D011392:Proline; D002374:Catalase; D005979:Glutathione Peroxidase; D007930:Leucine; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human
keywords: Catalase; gene polymorphism; glutathione peroxidase 1; reactive oxygen species; spontaneous abortion