title: Effect of mitochondrial ATP-sensitive potassium channel opening on the translocation of protein kinase C epsilon in adult rat ventricular myocytes.
abstract: This study aimed to investigate the effects of mitochondrial ATP-sensitive potassium (MitoKATP) channel opening on the translocation of protein kinase C epsilon (PKCε). In addition, we aimed to determine the relationship between PKCε translocation and the production of reactive oxygen species (ROS). PKCε protein expression in cultured adult rat ventricular myocytes was investigated by immunofluorescence and Western blotting. Diazoxide (DZ), a selective MitoKATP channel activator, caused a significant translocation to myofibrillar-like structures in cultured adult rat ventricular myocytes. N-2-Mercaptopropionylglycine, a free radical scavenger, could partially inhibit the translocation of PKCε induced by DZ. By contrast, chelerythrine, a selective PKC inhibitor, could completely block the translocation of PKCε induced by DZ. The opening of MitoKATP channels might activate and cause PKCε to translocate into myofibrillar-like structures. PKCε activation occurred downstream of the MitoKATP channel, possibly as a result of ROS production that occurred after the MitoKATP channels opened.
authors.affiliation: Department of Anesthesiology, Xinqiao Hospital, The Third Military Medical University, Chongqing, China lhdoccn@yeah.net.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Xinqiao Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: T
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, Xinqiao Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Long
authors.affiliation: Department of Anesthesiology, Xinqiao Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: J
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000818:Animals; D053119:Benzophenanthridines / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003981:Diazoxide / Q000494:pharmacology*; D054086:KATP Channels / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051744:Protein Kinase C-epsilon / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D008625:Tiopronin / Q000494:pharmacology
chemical_list: D053119:Benzophenanthridines; D054086:KATP Channels; D017382:Reactive Oxygen Species; D008625:Tiopronin; C016299:chelerythrine; C497257:Prkce protein, rat; D051744:Protein Kinase C-epsilon; D003981:Diazoxide