title: Bacterioplanes sanyensis gen. nov., sp. nov., a PHB-accumulating bacterium isolated from a pool of Spirulina platensis cultivation.
abstract: A Gram-negative, poly-3-hydroxybutyrate-accumulating rod bacterium, strain GYP-2(T), was isolated from a pool of marine Spirulina platensis cultivation, Sanya, China. Growth was observed at 10-45 °C and pH 6-10 in the presence of 1-10 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate belonged to Gammaproteobacteria and displayed 93.8-95.3 % 16S rRNA gene sequences similarities to members of the genera Thalassolituus, Oleibacter, and Oceanobacter, but house-keeping gene gyrB (encode DNA gyrase beta subunit) demonstrated that the new isolate was distantly related to Thalassolituus, Oleibacter, and Oceanobacter species (only 77-83 % gene gyrB sequences similarities).The G+C content of genomic DNA was 55 mol%. The major respiratory quinone was Q-9, while that for Oceanobacter kriegii LMG 6238(T) was Q-8. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. On the basis of its physiological, chemotaxonomic, and molecular properties, strain GYP-2(T) is suggested to represent a novel species of a new genus in Gammaproteobacteria, for which the name Bacterioplanes sanyensis gen. nov., sp. nov. is proposed. The type strain is GYP-2(T) (=CGMCC 1.12392(T)=KCTC 32220(T)).
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB-CAS), Guangdong Key Laboratory of Marine Materia Medica (LMMM-GD), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Wang
authors.forename: Qikun
authors.initials: Q
authors.lastname: Jia
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Shikun
authors.initials: S
authors.lastname: Dai
authors.forename: Huanlian
authors.initials: H
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.forename: Jiewei
authors.initials: J
authors.lastname: Fan
authors.forename: Wenzhou
authors.initials: W
authors.lastname: Xiang
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006885:Hydroxybutyrates / Q000378:metabolism*; D044130:Oceanospirillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010714:Phosphatidylethanolamines; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism*; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D053545:Spirulina / Q000254:growth & development; D000094122:Polyhydroxybutyrates
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D005227:Fatty Acids; D006885:Hydroxybutyrates; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D011091:Polyesters; D011809:Quinones; D012336:RNA, Ribosomal, 16S; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C483858:phosphatidylethanolamine