title: Amine functionalized nanodiamond promotes cellular adhesion, proliferation and neurite outgrowth.
abstract: In this study, we report the production of amine functionalized nanodiamond. The amine functionalized nanodiamond forms a conformal monolayer on a negatively charged surface produced via plasma polymerization of acrylic acid. Nanodiamond terminated surfaces were studied as substrates for neuronal cell culture. NG108-15 neuroblastoma-glioma hybrid cells were successfully cultured upon amine functionalized nanodiamond coated surfaces for between 1 and 7 d. Additionally, primary dorsal root ganglion (DRG) neurons and Schwann cells isolated from Wistar rats were also successfully cultured over a period of 21 d illustrating the potential of the coating for applications in the treatment of peripheral nerve injury.
authors.affiliation: Department of Materials Science and Engineering, The University of Sheffield, Broad Lane, Sheffield, S3 7HQ, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: Hopper
authors.forename: J M
authors.initials: JM
authors.lastname: Dugan
authors.forename: A A
authors.initials: AA
authors.lastname: Gill
authors.forename: O J L
authors.initials: OJ
authors.lastname: Fox
authors.forename: P W
authors.initials: PW
authors.lastname: May
authors.forename: J W
authors.initials: JW
authors.lastname: Haycock
authors.forename: F
authors.initials: F
authors.lastname: Claeyssens
mesh_terms: D000199:Actins / Q000737:chemistry; D000588:Amines / Q000737:chemistry*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D005463:Fluoroacetates / Q000737:chemistry; D005727:Ganglia, Spinal / Q000378:metabolism; D005910:Glioma / Q000188:drug therapy; D006801:Humans; D008297:Male; D058612:Nanodiamonds / Q000737:chemistry*; D036103:Nanotechnology; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism; D009447:Neuroblastoma / Q000188:drug therapy; D009474:Neurons / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D014269:Trifluoroacetic Acid / Q000737:chemistry
chemical_list: D000199:Actins; D000588:Amines; D005463:Fluoroacetates; D058612:Nanodiamonds; D014269:Trifluoroacetic Acid