title: Increased release time of antibiotics from bone allografts through a novel biodegradable coating.
abstract: The use of bone allografts is contraindicated in septic revision surgery due to the high risk of graft reinfection. Antibiotic release from the graft may solve the problem and these combinations can theoretically be used for prevention or even therapy of infection. The present study investigated whether amoxicillin, ciprofloxacin, and vancomycin alone or in combination with chitosan or alginate are suitable for short-term or long-term bone coating. Human bone allografts were prepared from femoral head and lyophilized. Antibiotic coating was achieved by incubating the grafts in antibiotic solution and freeze-drying again. Two biopolymers chitosan and alginate were used for creating sustained-release implantable coatings and the drug release profile was characterized in vitro by spectrophotometry. Using lyophilization with or without chitosan only resulted in short-term release that lasted up to 48 hours. Alginate coating enabled a sustained release that lasted for 8 days with amoxicillin, 28 days with ciprofloxacin coating, and 50 days with vancomycin coating. Using only implantable biodegradable allograft and polymers, a sustained release of antibiotics was achieved with ciprofloxacin and vancomycin for several weeks. Since the calculated daily release of the antibiotic was lower than the recommended IV dose, the calcium alginate coated bone graft can support endoprosthesis revision surgery.
authors.affiliation: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Tűzoltó utca 37-47, Budapest 1094, Hungary.
authors.forename: István
authors.identifier: 0000-0002-7183-8973
authors.initials: I
authors.lastname: Hornyák
authors.affiliation: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Tűzoltó utca 37-47, Budapest 1094, Hungary.
authors.forename: Edit
authors.initials: E
authors.lastname: Madácsi
authors.affiliation: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Tűzoltó utca 37-47, Budapest 1094, Hungary.
authors.forename: Pálma
authors.initials: P
authors.lastname: Kalugyer
authors.affiliation: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Tűzoltó utca 37-47, Budapest 1094, Hungary.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Vácz
authors.affiliation: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Tűzoltó utca 37-47, Budapest 1094, Hungary.
authors.forename: Dénes B
authors.initials: DB
authors.lastname: Horváthy
authors.affiliation: Department of Orthopedics, Semmelweis University, Karolina út 27, Budapest 1113, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Szendrői
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Fusionopolis Way, Singapore 138632.
authors.forename: Weiping
authors.initials: W
authors.lastname: Han
authors.affiliation: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Tűzoltó utca 37-47, Budapest 1094, Hungary ; Department of Orthopedics, Semmelweis University, Karolina út 27, Budapest 1113, Hungary.
authors.forename: Zsombor
authors.initials: Z
authors.lastname: Lacza
mesh_terms: D064591:Allografts / Q000187:drug effects*; D000658:Amoxicillin / Q000008:administration & dosage; D001672:Biocompatible Materials; D016025:Bone Transplantation / Q000009:adverse effects*; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry; D002939:Ciprofloxacin / Q000008:administration & dosage; D006801:Humans; D007239:Infections / Q000188:drug therapy* / Q000473:pathology; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry; D014640:Vancomycin / Q000008:administration & dosage
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D002939:Ciprofloxacin; D014640:Vancomycin; D000658:Amoxicillin; D048271:Chitosan