title: [Shenfu injection induces the apoptosis of prostate cancer PC-3 cells].
abstract: OBJECTIVE
  To study the effect of Shenfu Injection (SF) on the apoptosis of prostate cancer PC-3 cells and its possible mechanism.
  
  
  METHODS
  We divided prostate cancer PC-3 cells into a blank control group and three experimental groups, the latter treated with SF at 50, 100, and 200 microl/ml, respectively, for 24, 48, and 72 hours. Then we determined the proliferation of the cells by MTT assay, measured their apoptosis by Annexin V/PI flow cytometry, and detected the expression of P53 mRNA by RT-qPCR.
  
  
  RESULTS
  Compared with the blank control group, the survival rates of the prostate cancer PC-3 cells in the 50, 100, and 200 microl/ml SF groups were (93.76 +/- 2.63)%, (81.21 +/- 1.80)% and (18.01 +/- 3.84)% at 24 hours, (94.67 +/-1.11)%, (78.33 +/- 2.89)% and (10.34 +/- 1.44)% at48 hours, and (91.30 +/- 0.47)%, (36.67 +/- 1.56)% and (1.33 +/- 0.32)% at 72 hours, all significantly increased in a dose- and time-dependent manner (P < 0.05). The expression of p53 mRNA was also markedly increased in all the three experimental groups at 48 hours (P < 0. 05).
  
  
  CONCLUSIONS
  SF can inhibit the proliferation and induce the apoptosis of PC-3 cells, which may due to its upregulation of the p53 mRNA expression.
authors.forename: Li-Guo
authors.initials: LG
authors.lastname: Lü
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Zun-Guang
authors.initials: ZG
authors.lastname: Bai
authors.forename: Qiao-Ling
authors.initials: QL
authors.lastname: Wu
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Wang
authors.forename: Rui-Xin
authors.initials: RX
authors.lastname: Dai
authors.forename: Xiu-Qiong
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Si-Yi
authors.initials: SY
authors.lastname: Li
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; C492793:Shen-Fu; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53