title: Molecularly imprinted polymer on magnetic graphene oxide for fast and selective extraction of 17β-estradiol.
abstract: A novel nanosized substrate imprinted polymer (MIPs-GO-Fe3O4) was developed on a magnetic graphene oxide (GO-Fe3O4) surface for selective recognition and fast removal of 17β-estradiol (17β-E2). The characteristics of MIPs-GO-Fe3O4 were evaluated by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy. and vibrating sample magnetometer (VSM). Results suggested that GO had a thin single-layer structure anchoring Fe3O4 nanoparticles and that the imprinted film was coated on the GO-Fe3O4 surface. MIPs-GO-Fe3O4 was sensitive to the magnetic field and could be easily separated using an external magnet. The adsorption results indicated that the kinetic value and binding capacity of MIPs-GO-Fe3O4 were 0.0062 g (mg·min)(-1) and 4.378 μmol g(-1), respectively. The Langmuir-Freundlich isotherm and pseudo-second-order kinetic models were the main adsorption mechanisms for MIPs-GO-Fe3O4. MIPs-GO-Fe3O4 showed excellent recognition selectivity, as well as enrichment and separation abilities for 17β-E2 in complex matrices. MIPs-GO-Fe3O4 was also used to analyze 17β-E2 in real food samples, and satisfactory recoveries such as 84.20% with relative standard deviation (RSD) of 4.67% at a spike of 0.5 μmol L(-1) were obtained. Thus, the MIPs-GO-Fe3O4-based method provided a convenient and practical platform for the separation, enrichment, and removal of 17β-E2 in food samples.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang 330047, People's Republic of China.
authors.forename: Fangjian
authors.initials: F
authors.lastname: Ning
authors.forename: Hailong
authors.initials: H
authors.lastname: Peng
authors.forename: Jinhua
authors.initials: J
authors.lastname: Li
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Xiong
mesh_terms: D004958:Estradiol / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis; D006108:Graphite / Q000737:chemistry*; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D010087:Oxides; D011108:Polymers; D004958:Estradiol; D006108:Graphite