title: Significance of neuronal cytochrome P450 activity in opioid-mediated stress-induced analgesia.
abstract: Stressful environmental changes can suppress nociceptive transmission, a phenomenon known as "stress-induced analgesia". Depending on the stressor and the subject, opioid or non-opioid mechanisms are activated. Brain μ opioid receptors mediate analgesia evoked either by exogenous agents (e.g. morphine), or by the release of endogenous opioids following stressful procedures. Recent work with morphine and neuronal cytochrome P450 (P450)-deficient mice proposed a signal transduction role for P450 enzymes in µ analgesia. Since µ opioid receptors also mediate some forms of stress-induced analgesia, the present studies assessed the significance of brain P450 activity in opioid-mediated stress-induced analgesia. Two widely-used models of opioid stress-induced analgesia (restraint and warm water swim) were studied in both sexes of wild-type control and P450-deficient (Null) mice. In control mice, both stressors evoked moderate analgesic responses which were blocked by pretreatment with the opioid antagonist naltrexone, confirming the opioid nature of these responses. Consistent with literature, sex differences (control female>control male) were seen in swim-induced, but not restraint-induced, analgesia. Null mice showed differential responses to the two stress paradigms. As compared with control subjects, Null mice showed highly attenuated restraint-induced analgesia, showing a critical role for neuronal P450s in this response. However, warm water swim-induced analgesia was unchanged in Null vs. control mice. Additional control experiments confirmed the absence of morphine analgesia in Null mice. These results are the first to show that some forms of opioid-mediated stress-induced analgesia require brain neuronal P450 activity.
authors.affiliation: Center for Neuropharmacology and Neuroscience, Albany Medical College, Albany, NY, USA. Electronic address: houghl@mail.amc.edu.
authors.forename: Lindsay B
authors.initials: LB
authors.lastname: Hough
authors.affiliation: Center for Neuropharmacology and Neuroscience, Albany Medical College, Albany, NY, USA.
authors.forename: Julia W
authors.initials: JW
authors.lastname: Nalwalk
authors.affiliation: Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York at Albany, Albany, NY, USA.
authors.forename: Weizhu
authors.initials: W
authors.lastname: Yang
authors.affiliation: Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York at Albany, Albany, NY, USA.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Ding
mesh_terms: D000698:Analgesia; D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009020:Morphine / Q000494:pharmacology*; D009043:Motor Activity / Q000187:drug effects; D009474:Neurons / Q000201:enzymology; D059225:Nociception / Q000502:physiology; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000201:enzymology*; D013550:Swimming
chemical_list: D000701:Analgesics, Opioid; D009020:Morphine; D003577:Cytochrome P-450 Enzyme System
keywords: Analgesia; Brain; Cytochrome P450; Opioid; Stress; μ Opioid receptor