title: Nivulian-II a new milk clotting cysteine protease of Euphorbia nivulia latex.
abstract: Nivulian-II, new milk clotting cysteine protease has been purified from the latex of Euphorbia nivulia Buch.-Ham. Nivulian-II is a monomeric protein with an apparent molecular mass 43670.846 Da. It presents its optimum activity at pH 6.3 and temperature of 50°C. The enzyme was strongly inhibited by common thiol-blocking reagents thereby indicating that it belongs to cysteine protease family. Nivulian-II is a type of glycoprotein and its pI is 3.4. The N-terminal amino acid sequence of Nivulian-II is DFPPNTCCCICC. This sequence showed relatively low homology with several other proteases of Euphorbian plants, suggesting that the isolated enzyme is a new cysteine protease.
authors.affiliation: Department of Biochemistry, National Institute for Research in Reproductive Health (ICMR), Jehangir Merwanji Street, Parel, Mumbai 400012, Maharashtra, India; Faculty of Science, Department of Biotechnology, Moolji Jaitha College, North Maharashtra University, Jalgaon 425002, Maharashtra, India. Electronic address: sham83badgujar@gmail.com.
authors.forename: Shamkant B
authors.initials: SB
authors.lastname: Badgujar
authors.affiliation: Faculty of Science, Department of Biotechnology, Moolji Jaitha College, North Maharashtra University, Jalgaon 425002, Maharashtra, India.
authors.forename: Raghunath T
authors.initials: RT
authors.lastname: Mahajan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057056:Cysteine Proteases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004789:Enzyme Activation; D004795:Enzyme Stability; D028481:Euphorbia / Q000737:chemistry* / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007840:Latex / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D013696:Temperature
chemical_list: D007840:Latex; D057056:Cysteine Proteases
keywords: Cysteine protease; Euphorbia nivulia; Milk coagulation