title: ECD of tyrosine phosphorylation in a triple quadrupole mass spectrometer with a radio-frequency-free electromagnetostatic cell.
abstract: A radio frequency-free electromagnetostatic (EMS) cell devised for electron-capture dissociation (ECD) of ions has been retrofitted into the collision-induced dissociation (CID) section of a triple quadrupole mass spectrometer to enable recording of ECD product-ion mass spectra and simultaneous recording of ECD-CID product-ion mass spectra. This modified instrument can be used to produce easily interpretable ECD and ECD-CID product-ion mass spectra of tyrosine-phosphorylated peptides that cover over 50% of their respective amino-acid sequences and readily identify their respective sites of phosphorylation. ECD fragmentation of doubly protonated, tyrosine-phosphorylated peptides, which was difficult to observe with FT-ICR instruments, occurs efficiently in the EMS cell.
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis, OR, 97333, USA, valery.voinov@orst.edu.
authors.forename: Valery G
authors.initials: VG
authors.lastname: Voinov
authors.forename: Samuel E
authors.initials: SE
authors.lastname: Bennett
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Beckman
authors.forename: Douglas F
authors.initials: DF
authors.lastname: Barofsky
mesh_terms: D000595:Amino Acid Sequence; D004574:Electromagnetic Fields; D010455:Peptides / Q000737:chemistry*; D010766:Phosphorylation; D053719:Tandem Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D010455:Peptides; D014443:Tyrosine