title: Identification and crude protein extract of endophytic bacteria strain KLXD06 antagonistic against MRSA isolated from Hemsleya sinesis.
abstract: Hemsleya sinesis Cogn has a variety of medicinal value. Strain KLXD06 is an endophytic bacteria isolated from H. sinesis exhibited significant inhibitory activity against methicillin-resistant Staphylococcus aureus (MRSA). This strain was identified by methods including 16S rDNA sequence homology and phylogenetic analysis morphological, biochemical and physiological characteristics analysis. Ccrude protein from KLXD06 was extracted by ammon ium sulfate salting-out. The results showed that strain KLXD06 was identified as Serratia marcescens. Antibacterial crude protein from KLXD06 was extracted by ammon ium sulfate salting-out, has a thermal stability.
authors.affiliation: School of Life Sciences, Leshan Normal University, Leshan, Sichuan, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Tang
authors.affiliation: School of Life Sciences, Leshan Normal University, Leshan, Sichuan, China.
authors.forename: Mingfu
authors.initials: M
authors.lastname: Gong
authors.affiliation: School of Life Sciences, Leshan Normal University, Leshan, Sichuan, China.
authors.forename: Chenghao
authors.initials: C
authors.lastname: Xu
authors.affiliation: School of Life Sciences, Leshan Normal University, Leshan, Sichuan, China.
authors.forename: Sanlin
authors.initials: S
authors.lastname: Wu
authors.affiliation: School of Life Sciences, Leshan Normal University, Leshan, Sichuan, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
mesh_terms: D001426:Bacterial Proteins / Q000494:pharmacology*; D019662:Cucurbitaceae / Q000382:microbiology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D010802:Phylogeny; D012706:Serratia marcescens / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins