title: Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation.
abstract: Extracellular vesicles (EVs) facilitate intercellular communication by carrying bioactive molecules such as proteins, messenger RNA, and micro (mi)RNAs. Recently, high-density lipoproteins (HDL) isolated from human plasma were also reported to transport miRNA to other cells. HDL, when isolated from human plasma, ranges in density between 1.063 and 1.21 g/mL, which grossly overlap with the reported density of EVs. Consequently, HDL and EV will be co-isolated when using density gradient ultracentrifugation. Thus, more stringent isolation/separation procedures of EV and HDL are essential to know their relative contribution to the pool of circulating bioactive molecules.
authors.affiliation: Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Yuana
authors.initials: Y
authors.lastname: Yuana
authors.affiliation: Department of Experimental Vascular Medicine, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Johannes
authors.initials: J
authors.lastname: Levels
authors.affiliation: Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Anita
authors.initials: A
authors.lastname: Grootemaat
authors.affiliation: Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Auguste
authors.initials: A
authors.lastname: Sturk
authors.affiliation: Department of Clinical Chemistry, Academic Medical Centre of the University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Rienk
authors.initials: R
authors.lastname: Nieuwland
keywords: Isolation; RNA; extracellular vesicles; lipoproteins