title: Magnetic graphene oxide as adsorbent for the determination of polycyclic aromatic hydrocarbon metabolites in human urine.
abstract: Detection of monohydroxy polycyclic aromatic hydrocarbons metabolites in urine is an advisable and valid method to assess human environmental exposure to polycyclic aromatic hydrocarbons. In this work, novel Fe3O4/graphene oxide composites were prepared and their application in the magnetic solid-phase extraction of monohydroxy polycyclic aromatic hydrocarbons in urine was investigated by coupling with liquid chromatography and mass spectrometry. In the hybrid material, superparamagnetic Fe3O4 nanoparticles provide fast separation to simplify the analytical process and graphene oxide provides a large functional surface for the adsorption. The prepared magnetic nanocomposites were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and vibrating sample magnetometry. The experimental conditions were optimized systematically. Under the optimal conditions, the recoveries of these compounds were in the range of 98.3-125.2%, the relative standard deviations ranged between 6.8 and 15.5%, and the limits of detection were in the range of 0.01-0.15 ng/mL. The simple, quick, and affordable method was successfully used in the analysis of human urinary monohydroxy polycyclic aromatic hydrocarbons in two different cities. The results indicated that the monohydroxy polycyclic aromatic hydrocarbons level in human urine can provide useful information for environmental exposure to polycyclic aromatic hydrocarbons.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Institute of Environmental Chemistry, College of Chemistry, Central China Normal University, Wuhan, China.
authors.forename: Linli
authors.initials: L
authors.lastname: Zhu
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism / Q000652:urine*; D052616:Solid Phase Extraction; D013499:Surface Properties; D055815:Young Adult
chemical_list: D058185:Magnetite Nanoparticles; D010087:Oxides; D011084:Polycyclic Aromatic Hydrocarbons; D006108:Graphite
keywords: Graphene oxide; Liquid chromatography with mass spectrometry; Magnetic solid-phase extraction; Monohydroxy polycyclic aromatic hydrocarbons; Urine