title: Cross-reactivity among non-specific lipid-transfer proteins from food and pollen allergenic sources.
abstract: Non-specific lipid-transfer proteins (nsLTPs) are a family of pan-allergens present in foods and pollen. However, sequence homology among them is limited. The objective of this study was to evaluate the IgE-mediated cross-reactivity between nsLTPs from different sources and evaluate the allergenic properties of LTPs from peach (Pru p 3) and pellitory (Par j 1/Par j 2), major fruit and pollen allergens. Both proteins were purified and characterised. Cross-reactivity studies among nsLTPs from different foods and pollens were performed by immunoblot inhibition using sera specific to peach or pellitory pollen. Cross-reactivity with Pru p 3 was observed in hazelnut, onion, corn, peanut and apple while in pollens, none of the extracts was inhibited with Par j 1/2. In conclusion, Pru p 3 did not inhibit LTPs from most fruits. Therefore, although Pru p 3 covers the largest number of epitopes, diagnosis with only this allergen may not detect all LTP sensitivities.
authors.affiliation: R&D Department, Laboratorios LETI, Calle del Sol, 5, 28760 Tres Cantos (Madrid), Spain.
authors.forename: María
authors.initials: M
authors.lastname: Morales
authors.affiliation: R&D Department, Laboratorios LETI, Calle del Sol, 5, 28760 Tres Cantos (Madrid), Spain.
authors.forename: M Ángeles
authors.initials: MÁ
authors.lastname: López-Matas
authors.affiliation: R&D Department, Laboratorios LETI, Calle del Sol, 5, 28760 Tres Cantos (Madrid), Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Moya
authors.affiliation: R&D Department, Laboratorios LETI, Calle del Sol, 5, 28760 Tres Cantos (Madrid), Spain. Electronic address: jcarnes@leti.com.
authors.forename: Jerónimo
authors.initials: J
authors.lastname: Carnés
mesh_terms: D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D003429:Cross Reactions; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology*
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D007073:Immunoglobulin E
keywords: Cross-reactivity; Food allergy; LTP; Panallergens