title: Calcium signaling mediates the response to cadmium toxicity in Saccharomyces cerevisiae cells.
abstract: The involvement of Ca(2+) in the response to high Mn(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Cd(2+), and Hg(2+) was investigated in Saccharomyces cerevisiae. The yeast cells responded through a sharp increase in cytosolic Ca(2+) when exposed to Cd(2+), and to a lesser extent to Cu(2+), but not to Mn(2+), Co(2+), Ni(2+), Zn(2+), or Hg(2+). The response to high Cd(2+) depended mainly on external Ca(2+) (transported through the Cch1p/Mid1p channel) but also on vacuolar Ca(2+) (released into the cytosol through the Yvc1p channel). The adaptation to high Cd(2+) was influenced by perturbations in Ca(2+) homeostasis. Thus, the tolerance to Cd(2+) often correlated with sharp Cd(2+)-induced cytosolic Ca(2+) pulses, while the Cd(2+) sensitivity was accompanied by the incapacity to rapidly restore the low cytosolic Ca(2+).
authors.affiliation: University of Bucharest, Faculty of Chemistry, Sos. Panduri 90-92, 050663 Bucharest, Romania.
authors.forename: Lavinia L
authors.initials: LL
authors.lastname: Ruta
authors.affiliation: University of Bucharest, Faculty of Chemistry, Sos. Panduri 90-92, 050663 Bucharest, Romania.
authors.forename: Valentina C
authors.initials: VC
authors.lastname: Popa
authors.affiliation: University of Bucharest, Faculty of Chemistry, Sos. Panduri 90-92, 050663 Bucharest, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Nicolau
authors.affiliation: University of Bucharest, Faculty of Chemistry, Sos. Panduri 90-92, 050663 Bucharest, Romania.
authors.forename: Andrei F
authors.initials: AF
authors.lastname: Danet
authors.affiliation: University of Bucharest, Faculty of Biology, Spl. Independentei 91-95, 050095 Bucharest, Romania.
authors.forename: Virgil
authors.initials: V
authors.lastname: Iordache
authors.affiliation: University of Bucharest, Faculty of Biology, Spl. Independentei 91-95, 050095 Bucharest, Romania.
authors.forename: Aurora D
authors.initials: AD
authors.lastname: Neagoe
authors.affiliation: University of Bucharest, Faculty of Chemistry, Sos. Panduri 90-92, 050663 Bucharest, Romania. Electronic address: ileana.farcasanu@g.unibuc.ro.
authors.forename: Ileana C
authors.initials: IC
authors.lastname: Farcasanu
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects*; D027682:Cation Transport Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D000079922:Copper Transport Proteins; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D006706:Homeostasis / Q000187:drug effects; D033862:Iron-Binding Proteins / Q000378:metabolism; D009154:Mutation; D018384:Oxidative Stress / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D027682:Cation Transport Proteins; D000079922:Copper Transport Proteins; D004785:Environmental Pollutants; C105847:FET4 protein, S cerevisiae; D033862:Iron-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; D002104:Cadmium; D002118:Calcium
keywords: Aequorin; Cadmium; Calcium signaling; Heavy metal stress; Saccharomyces cerevisiae