title: Enhanced fluorescence quenching in an acridine orange - alizarin red system through matrine and its analytical application.
abstract: This study shows that alizarin red (AR) only slightly quenched fluorescence for acridine orange (AO) in an AR/AO mixed solution at pH=5-6. The reduced fluorescent signal was closely and linearly associated with the level of MT added to the system, which is the basis for a new quantitative MT assay method using the fluorescence quenching reaction in the AO-AR system. The results show that under optimal conditions, this method had a 14.9-43.5 mg L(-1) linear detection range with a 1.38 mg L(-1) detection limit and 1.24% precision. In addition, this method was used to determine the MT levels in the commercially available MT-containing pesticides and suppositories, which showed a 96.6-103% recovery. Therefore, this method has multiple advantages, including simple and fast operation, high accuracy and low cost. Moreover, herein, we investigated the underlying mechanism in-depth using an ultraviolet (UV) spectroscopic technique.
authors.affiliation: College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wei
authors.affiliation: College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Gong
authors.affiliation: College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. Electronic address: wxl2558@163.com.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
authors.forename: Shiwu
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000165:Acridine Orange / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis*; D000880:Anthraquinones / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D057230:Limit of Detection; D011807:Quinolizines / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000880:Anthraquinones; D005456:Fluorescent Dyes; D011807:Quinolizines; C010078:alizarin; D000165:Acridine Orange; D000093842:Matrines
keywords: Acridine orange (AO); Alizarin red (AR); Energy transfer; Fluorescence quenching; Matrine