title: An electrochemically switched smart surface for peptide immobilization and conformation control.
abstract: We report an electrochemically switched smart surface for controlled peptide immobilization and conformation control. This dynamic surface is based on self-assembled monolayers (SAMs) containing surface-bound trimethoxybenzene moieties, which can undergo electrochemically modulated surface activation to be stepwisely converted to two catechol derivatives. This new smart surface can be used to realize stepwise immobilization of a peptide, and more importantly, to control peptide conformation on a surface. We demonstrate herein that with one electrochemical activation step, a linear peptide containing an RGD sequence can be attached onto the SAMs. With the subsequence activation step, the attached linear RGD peptide can be converted into cyclic conformation. The SAMs bounded with linear and cyclic RGD exhibit different adhesion behaviors to fibroblasts cells. The reaction procedure can be well-monitored by cyclic voltammetry (CV), electrochemical surface enhanced Raman microscopy (EC-SERS), and X-ray photoelectron spectroscopy (XPS). It is believed this robust smart surface can find wide applications in surface immobilization of bioactive moieties.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry (SKLAOC), College of Chemistry and Chemical Engineering, ‡School of Basic Medical Sciences Lanzhou University , 222 Tianshui South Road, Lanzhou, 730000 China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Sun
authors.forename: Rong
authors.initials: R
authors.lastname: Shen
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Cao
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: De-Cheng
authors.initials: DC
authors.lastname: Bai
authors.forename: Hao-Li
authors.initials: HL
authors.lastname: Zhang
mesh_terms: D001554:Benzene / Q000737:chemistry; D003500:Cyclization; D004563:Electrochemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D011487:Protein Conformation; D013499:Surface Properties
chemical_list: D055767:Immobilized Proteins; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D001554:Benzene