title: Candida galli as a cause of tinea unguium-molecular characterization of a rare clinical fungal entity.
abstract: We present the first clinical report of an infection caused by Candida galli, an anamorphic yeast species in the Yarrowia clade. C. galli has been described in the literature only four times, but never before it has been isolated from clinical samples. The colony morphology on Sabouraud medium and morphotype on CHROMagar Candida medium were similar to C. lipolytica as well as the carbon assimilation profile. The phenotypic differences with C. lipolytica were the non-assimilation of N-acetyl glucosamine, the absence of urease activity, growth in 10 % NaCl with 5 % glucose and in vitamin-free medium. MALDI-TOF MS could not generate reliable identification of the strain. Molecular analysis based on amplification of the ITS1-5.8S-ITS2 rDNA regions confirmed the identity as C. galli. Antifungal susceptibility test clearly demonstrated high MICs to 5-fluorocytosine, amphotericin B and fluconazole, as in the species belonging to the Yarrowia clade.
authors.affiliation: Clinical Microbiology Laboratory, Puerta del Mar University Hospital, Cádiz, Spain, fatima.galan@uca.es.
authors.forename: Fátima
authors.initials: F
authors.lastname: Galán-Sánchez
authors.forename: Lidia
authors.initials: L
authors.lastname: García-Agudo
authors.forename: Pedro
authors.initials: P
authors.lastname: García-Martos
authors.forename: Manuel
authors.initials: M
authors.lastname: Rodríguez-Iglesias
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques; D008875:Middle Aged; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D014009:Onychomycosis / Q000175:diagnosis* / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012965:Sodium Chloride; D002244:Carbon