title: Differential expression of cholecystokinin A receptor in gallbladder cancer in the young and elderly suggests two subsets of the same disease?
abstract: BACKGROUND
  Cholecystokinin type A receptor (CCKAR) is known to be overexpressed in variety of human malignancies but information regarding its expression in gallbladder cancer (GBC) is limited. Attempts were now made to investigate expression pattern of CCKAR mRNA and protein in controls and GBC patients and correlate it with various clinicopathological parameters following surgical resection.
  
  
  METHODS
  Gallbladder tissue samples from 64 subjects (GBC: 39; control: 25) were studied. Expression of CCKAR mRNA was evaluated by reverse transcriptase-polymerase chain reaction and confirmed using real-time polymerase chain reaction. Protein expression was studied by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Significantly higher expression of CCKAR mRNA (P < 0.0001) and protein (P < 0.0001) was observed in GBC tissues. Overexpression was also observed for stage III and in moderately and poorly differentiated tumors. When the clinicopathological parameters were compared, we found age dependent decrease in CCKAR expression. Relatively higher expression of CCKAR was observed in younger patients (age < 45 years) having more aggressive disease when compared with elderly ones (age ≥ 45 years).
  
  
  CONCLUSIONS
  Age related differential expression of CCKAR in GBC may suggest two possible variants of the disease in this endemic belt.
authors.affiliation: Department of Surgical Gastroenterology, King George's Medical University, Lucknow 226003, India.
authors.forename: Hasan Raza
authors.initials: HR
authors.lastname: Kazmi
authors.affiliation: Department of Surgical Gastroenterology, King George's Medical University, Lucknow 226003, India.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chandra
authors.affiliation: Department of Surgical Gastroenterology, King George's Medical University, Lucknow 226003, India.
authors.forename: Kavita
authors.initials: K
authors.lastname: Baghel
authors.affiliation: Developmental Toxicology Division, Indian Institute of Toxicology Research, Lucknow 226003, India.
authors.forename: Anshuman
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Surgical Gastroenterology, King George's Medical University, Lucknow 226003, India.
authors.forename: Jaya
authors.initials: J
authors.lastname: Nigam
authors.affiliation: Developmental Toxicology Division, Indian Institute of Toxicology Research, Lucknow 226003, India.
authors.forename: Devendra
authors.initials: D
authors.lastname: Parmar
authors.affiliation: Department of Biochemistry, King George's Medical University, Lucknow 226003, India.
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Mahdi
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh 462024, India.
authors.forename: Sudhir K
authors.initials: SK
authors.lastname: Goel
authors.affiliation: All India Institute of Medical Sciences, Bhopal, Madhya Pradesh 462024, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000096:biosynthesis*; D043886:Receptor, Cholecystokinin A / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D043886:Receptor, Cholecystokinin A