title: Bioinformatic analysis and characteristics of glycoprotein C encoded by the newly identified UL44 gene of duck plague virus.
abstract: Glycoprotein C is one of the duck plague virus (DPV) glycoproteins and is encoded by the DPV UL44 gene. DPV glycoprotein C (DPV-gC) comprises 431 amino acids with a putative molecular mass of 47.35 kDa. Sequence analysis indicated that the protein possesses typical characteristics of type-I membrane glycoproteins, containing an N-terminal signal sequence, an external domain, a C-terminal membrane anchor region, and a short cytoplasmic domain. Comparisons of 22 alphaherpesvirus-gC protein sequences revealed eight conservative Cys-residue sites, which may play a crucial role in the biological functions and structural stabilization of the DPV-gC protein. Estimates of potential antigenic epitopes and secondary structure identified four B cell dominant epitopes, which are located at amino acids 68-71, 87-91, 369-352, and 372-374. A model for the structure of DPV-gC was derived by associating its predicted secondary and three-dimensional structures. In conclusion, these results will provide a basis for further functional studies of DPV-gC, establishing novel clinical diagnoses of DPV, and in the development of a new DPV vaccine.
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, China.
authors.forename: K F
authors.initials: KF
authors.lastname: Sun
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, China chenganchun@vip.163.com.
authors.forename: A C
authors.initials: AC
authors.lastname: Cheng
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, China.
authors.forename: M S
authors.initials: MS
authors.lastname: Wang
mesh_terms: D018138:Alphaherpesvirinae / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods*; D018604:Epitope Mapping; D005814:Genes, Viral; D008958:Models, Molecular; D010802:Phylogeny; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D014759:Viral Envelope Proteins