title: Enantioselective high performance liquid chromatography and supercritical fluid chromatography separation of spirocyclic terpenoid flavor compounds.
abstract: Chiral spirocyclic terpenoids are abundant natural flavors with significant impact particularly on the food industry. Chromatographic methods for analytical and preparative separation of these compounds are therefore of high interest to natural product chemists in academia and industry. Gas chromatography on chiral stationary phases is currently the standard method for the separation of volatile terpenoids, limiting the scale to analytical quantities. We report herein high performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) protocols for the chiral separation of several racemic spirocyclic terpenoids such as the important flavors theaspirane and vitispirane. A screening of mobile phases and 16 commercially available chiral stationary phases (CSPs) largely based on polysaccharides led to identification of protocols for the separation of all terpenoids tested. SFC methods were found to be particularly useful for the separation of these spirocyclic flavors due to the volatility and low polarity of the compounds. The reported chiral HPLC and SFC protocols are scalable alternatives to gas chromatographic separations of volatile terpenoid flavors.
authors.affiliation: Sanofi, LGCR Chemistry, 65926 Frankfurt am Main, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Schaffrath
authors.affiliation: University of Hamburg, Department of Chemistry, Pharmaceutical and Medicinal Chemistry, Bundesstraße 45, 20146 Hamburg, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Weidmann
authors.affiliation: University of Hamburg, Department of Chemistry, Pharmaceutical and Medicinal Chemistry, Bundesstraße 45, 20146 Hamburg, Germany. Electronic address: maison@chemie.uni-hamburg.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Maison
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D013237:Stereoisomerism; D013729:Terpenes / Q000032:analysis*
chemical_list: D013729:Terpenes
keywords: Allylic oxidation; Chiral separation; Chiral stationary phases; Flavors; Natural products; Terpenes