title: A gene variant in the transcription factor 7-like 2 (TCF7L2) is associated with an increased risk of gestational diabetes mellitus.
abstract: OBJECTIVE
  Adipokines play an important role in the pathogenesis of insulin resistance during pregnancy. We studied the association of genetic variants linked with type 2 diabetes in gestational diabetes mellitus (GDM) subjects and its influence on maternal adipokines.
  
  
  METHODS
  We recruited 25 healthy pregnant women (Controls) and 45 women with GDM at 24-28 weeks of gestation. Maternal blood samples were collected at recruitment and delivery. Adipokines were determined at both sampling times. Genomic DNA was extracted from recruitment samples and FTO rs9939609, TCF7L2 rs4506565, rs7901695, rs12243326, rs12255372 and rs7903146, INSIG2 rs7566605, SREBF1 rs114001633, rs45535737 and rs12941356 and FATP4 rs2003560 genotyped.
  
  
  RESULTS
  Serum adiponectin was significantly lower in GDM than Controls at recruitment and showed a similar trend at delivery (p=0.060). In contrast, resistin tended to higher levels in GDM only at recruitment. TCF7L2 rs4506565 (OR=2.31, 95% CI: 1.97-5.01; p=0.031) and FTO rs9939609 (OR=2.17, 95% CI: 1.07-4.41; p=0.039) were associated with GDM risk. Women carrying the T allele of TCF7L2 rs4506565 had increases in plasma resistin of 9.38 μg/L (95% CI 1.39-17.37; p=0.022) per allele; this association remained significant after adjusting for pre-gestational body weight.
  
  
  CONCLUSIONS
  TCF7L2 rs4506565 variant (T/T) is associated with increased risk of GDM and plasma resistin concentrations in women with GDM.
authors.affiliation: Physiology Department, Faculty of Biology, University of Murcia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Pagán
authors.affiliation: Clinical Analysis Service, Virgen de la Arrixaca Hospital, Murcia, Spain. Electronic address: mariasm@um.es.
authors.forename: María
authors.initials: M
authors.lastname: Sabater-Molina
authors.affiliation: Department of Biochemistry and Molecular Biology II, University of Granada, Spain.
authors.forename: Josune
authors.initials: J
authors.lastname: Olza
authors.affiliation: Gynecology Service, Virgen de la Arrixaca Hospital, Murcia, Spain.
authors.forename: María T
authors.initials: MT
authors.lastname: Prieto-Sánchez
authors.affiliation: Gynecology Service, Virgen de la Arrixaca Hospital, Murcia, Spain.
authors.forename: Jose E
authors.initials: JE
authors.lastname: Blanco-Carnero
authors.affiliation: Gynecology Service, Virgen de la Arrixaca Hospital, Murcia, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Parrilla
authors.affiliation: Department of Biochemistry and Molecular Biology II, University of Granada, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Gil
authors.affiliation: Physiology Department, Faculty of Biology, University of Murcia, Spain.
authors.forename: Elvira
authors.initials: E
authors.lastname: Larqué
mesh_terms: D052242:Adiponectin / Q000097:blood; D000328:Adult; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; D016022:Case-Control Studies; D016640:Diabetes, Gestational / Q000097:blood / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011506:Proteins / Q000235:genetics*; D052243:Resistin / Q000097:blood; D058118:Transcription Factor 7-Like 2 Protein / Q000235:genetics*
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D011506:Proteins; C499624:RETN protein, human; D052243:Resistin; C544894:TCF7L2 protein, human; D058118:Transcription Factor 7-Like 2 Protein; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human
keywords: Adiponectin; Gestational diabetes mellitus; Resistin; Single-nucleotide polymorphism; TCF7L2