title: Morphologic and morphometric analysis of alternations in the oral cavity caused by Candida albicans--experimental work.
abstract: BACKGROUND
  Candidiasis has become a human disease of increasing importance in the last decades. The aim of the study is to establish pathomorphological alterations caused by the blastospores of the Candida albicans as well as morphometric alterations.
  
  
  METHODS
  The experiment was carried out on 2.5-month-old rats, weighting 110-130 g. The study sample was divided into the animals infected by a submucous inoculation in the periodontal region and the controls. The gingival specimens were taken, preparations were done and stained by the hematoxylin-eosin and Periodic acid Schiff methods.
  
  
  RESULTS
  The following alterations were found out by the stereological analysis: an average volume of nuclei of the gingival epithelial cells was 111.82 microm3 (SD = 25.34) on the first day. A statistically significant increase in the volume of nuclei in the experimental group began to occur from the fourth day (202.97 microm3; SD = 31.16, p < 0.05) and the highest value of the nuclei volume was found out on the eight day of the experiment (316.83 microm3; SD = 40.15).
  
  
  CONCLUSIONS
  Blastospores of Candida albicans are pathogenic for the gingival tissue where they cause degenerative necrotic alterations of the granulomatous character and after the fourth day from the inoculation, the development of the pseudohyphae was observed. The obtained values of stereologic measurement show the acute increase in the volume of nuclei.
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Kesić
authors.forename: Radojka
authors.initials: R
authors.lastname: Delić
authors.forename: Dragan
authors.initials: D
authors.lastname: Mihailović
authors.forename: Milica S
authors.initials: MS
authors.lastname: Petrović
authors.forename: Tijana D
authors.initials: TD
authors.lastname: Delić
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000254:growth & development* / Q000378:metabolism; D002180:Candidiasis, Oral / Q000378:metabolism / Q000382:microbiology*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D005881:Gingiva / Q000378:metabolism / Q000382:microbiology*; D051381:Rats