title: Inhibition of Hepatitis B virus replication by SAMHD1.
abstract: Sterile alpha motif and HD-domain containing protein 1 (SAMHD1) is a newly identified intracellular antiviral factor. By depleting the dNTPs pool of host cells to a low level that cannot support the efficient synthesis of viral cDNA, it restricts replication of some retroviruses. As a DNA virus, Hepatitis B virus (HBV) experiences a process of reverse transcription in its life cycle akin to that of retroviruses. However, whether SAMHD1 can restrict HBV replication in liver cells is unknown. Here, we reported that SAMHD1 expression was detectable in four liver cell lines. Exogenous expression of SAMHD1 in SMMC-7721 cells restrained HBV replication. Similarly, SAMHD1 impeded HBV replication in another liver cell line, BEL-7402. Remarkably, the catalytically inactive mutant, SAMHD1 HD/AA also hampered HBV replication. Additionally, HBV replication reduced SAMHD1 expression in HepG2 cells. Moreover, it was found that IFN-α induced expression of SAMHD1 in liver cells. Together, these findings suggested that IFN-α-inducible SAMHD1 inhibited HBV replication in liver cells.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Zhangming
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Mengying
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Yongji
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Yan
authors.affiliation: School of Basic Medical Sciences and Biopharmaceutical Research Institute, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Tongcui
authors.initials: T
authors.lastname: Jiang
authors.affiliation: School of Basic Medical Sciences and Biopharmaceutical Research Institute, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Xiaowan
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province 230032, China.
authors.forename: Jinsen
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui Province 230032, China. Electronic address: yingsc@ahmu.edu.cn.
authors.forename: Songcheng
authors.initials: S
authors.lastname: Ying
authors.affiliation: School of Basic Medical Sciences and Biopharmaceutical Research Institute, Anhui Medical University, Hefei, Anhui Province 230032, China. Electronic address: shenyx@ahmu.edu.cn.
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Shen
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D016898:Interferon-alpha / Q000502:physiology; D020559:Monomeric GTP-Binding Proteins / Q000502:physiology*; D000076106:SAM Domain and HD Domain-Containing Protein 1; D014779:Virus Replication / Q000502:physiology*
chemical_list: D016898:Interferon-alpha; D000076106:SAM Domain and HD Domain-Containing Protein 1; C484280:SAMHD1 protein, human; D020559:Monomeric GTP-Binding Proteins
keywords: HBV; IFN-α; Liver cell; SAMHD1