title: Genome-wide determination of poly(A) sites in Medicago truncatula: evolutionary conservation of alternative poly(A) site choice.
abstract: BACKGROUND
  Alternative polyadenylation (APA) plays an important role in the post-transcriptional regulation of gene expression. Little is known about how APA sites may evolve in homologous genes in different plant species. To this end, comparative studies of APA sites in different organisms are needed. In this study, a collection of poly(A) sites in Medicago truncatula, a model system for legume plants, has been generated and compared with APA sites in Arabidopsis thaliana.
  
  
  RESULTS
  The poly(A) tags from a deep-sequencing protocol were mapped to the annotated M. truncatula genome, and the identified poly(A) sites used to update the annotations of 14,203 genes. The results show that 64% of M. truncatula genes possess more than one poly(A) site, comparable to the percentages reported for Arabidopsis and rice. In addition, the poly(A) signals associated with M. truncatula genes were similar to those seen in Arabidopsis and other plants. The 3'-UTR lengths are correlated in pairs of orthologous genes between M. truncatula and Arabidopsis. Very little conservation of intronic poly(A) sites was found between Arabidopsis and M. truncatula, which suggests that such sites are likely to be species-specific in plants. In contrast, there is a greater conservation of CDS-localized poly(A) sites in these two species. A sizeable number of M. truncatula antisense poly(A) sites were found. A high percentage of the associated target genes possess Arabidopsis orthologs that are also associated with antisense sites. This is suggestive of important roles for antisense regulation of these target genes.
  
  
  CONCLUSIONS
  Our results reveal some distinct patterns of sense and antisense poly(A) sites in Arabidopsis and M. truncatula. In so doing, this study lends insight into general evolutionary trends of alternative polyadenylation in plants.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wu
authors.forename: Bobby
authors.initials: B
authors.lastname: Gaffney
authors.affiliation: Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, USA. aghunt00@uky.edu.
authors.forename: Arthur G
authors.initials: AG
authors.lastname: Hunt
authors.forename: Qingshun Q
authors.initials: QQ
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D017360:Arabidopsis / Q000235:genetics; D005075:Biological Evolution; D018745:Genome, Plant; D059014:High-Throughput Nucleotide Sequencing; D007438:Introns; D046913:Medicago truncatula / Q000145:classification / Q000235:genetics*; D012275:Oryza / Q000235:genetics; D011061:Poly A / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D017422:Sequence Analysis, DNA
chemical_list: D020413:3' Untranslated Regions; D011061:Poly A