title: Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis.
abstract: A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1mL of deionized water. Double opposite end injection capillary electrophoresis with contactless conductometric detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3min in a background electrolyte containing 20mM 2-(N-morpholino)ethanesulfonic acid, 20mM l-histidine and 2mM 18-crown-6. By using the target ion ratios rather than the concentrations of each individual ion combined with principal component analysis, the diagnosis of CF can be made more accurately and greatly reduce the number of false positive or negative results as is often the case when single ion (chloride) is analyzed.
authors.affiliation: Bioanalytical instrumentation, CEITEC Masaryk University, Veveri 97, 602 00 Brno, Czech Republic. Electronic address: petr.kuban@ceitec.muni.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Kubáň
authors.affiliation: Bioanalytical instrumentation, CEITEC Masaryk University, Veveri 97, 602 00 Brno, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Greguš
authors.affiliation: Department of Respiratory Diseases and TB, University Hospital Brno and Faculty of Medicine, Masaryk University Brno, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Pokojová
authors.affiliation: Department of Respiratory Diseases and TB, University Hospital Brno and Faculty of Medicine, Masaryk University Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Skřičková
authors.affiliation: Bioanalytical instrumentation, CEITEC Masaryk University, Veveri 97, 602 00 Brno, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Foret
mesh_terms: D016022:Case-Control Studies; D002712:Chlorides / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000175:diagnosis / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods; D005189:False Positive Reactions; D006801:Humans; D011188:Potassium / Q000378:metabolism*; D025341:Principal Component Analysis; D012964:Sodium / Q000378:metabolism*; D013542:Sweat / Q000378:metabolism*
chemical_list: D002712:Chlorides; D012964:Sodium; D011188:Potassium
keywords: Capillary electrophoresis; Contactless conductivity detection; Cystic fibrosis; Ions; Principal component analysis