title: Prognostic significance of γ-H2AX in laryngeal squamous cell carcinoma after surgery.
abstract: BACKGROUND
  The clinical significance of γ-H2AX in laryngeal squamous cell carcinoma (LSCC) has not yet been established. This study was performed to assess the expression of nuclear γ-H2AX in benign and malignant laryngeal lesions and to assess its clinicopathological significance.
  
  
  METHODS
  A total of 70 LSCC tumor-normal tissue paired samples were evaluated for γ-H2AX expression using immunohistochemical staining. Their expression was correlated with different clinicopathological parameters.
  
  
  RESULTS
  Nuclear γ-H2AX expression was frequently detected in LSCC tissues (P < 0.001). High nuclear γ-H2AX levels were not associated with any clinicopathological characteristics of LSCC (P > 0.05). Univariate Kaplan-Meier analysis showed that positive nuclear γ-H2AX expression was associated with a decreased overall survival (P = 0.017). Multivariate Cox regression analysis showed that nuclear γ-H2AX expression was an independent risk factor for overall survival.
  
  
  CONCLUSIONS
  The expression of nuclear γ-H2AX might be closely related to the prognosis of LSCC.
authors.affiliation: Department of ENT, Eye, Ear, Nose & Throat Hospital, Fudan University, Shanghai 200437, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of ENT, Eye, Ear, Nose & Throat Hospital, Fudan University, Shanghai 200437, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of ENT, Eye, Ear, Nose & Throat Hospital, Fudan University, Shanghai 200437, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D006657:Histones / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: C468783:H2AX protein, human; D006657:Histones