title: The Cu²⁺ binding properties of branched peptides based on L-2,3-diaminopropionic acid.
abstract: Three new branched peptides, namely, H-Gly-Dap(H-Gly)-Gly-NH2 (3G), H-His-Dap(H-His)-Gly-NH2 (2HG), and H-Gly-Dap(H-Gly)-His-NH2 (2GH), where Dap stands for the 2,3-diaminopropionic acid residue, were synthesized by solid phase procedures. Because of the junction at Dap these peptides have three available pending arms for metal chelation. The complex formation between these peptides and 1 equiv of Cu(2+) was investigated as a function of pH by potentiometry ultraviolet-visible absorption, circular dichroism, and X-band electron paramagnetic resonance spectroscopy in aqueous medium. Our results clearly demonstrate that cooperation between all three peptide arms essentially contributes to the stability of copper(II) complexes.
authors.affiliation: CNRS/UPPA, LCABIE, UMR5254 , Hélioparc, 2, av. Pr. Angot, F-64053 Pau, France.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Szyrwiel
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Szczukowski
authors.forename: József S
authors.initials: JS
authors.lastname: Pap
authors.forename: Bartosz
authors.initials: B
authors.lastname: Setner
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Szewczuk
authors.forename: Wiesław
authors.initials: W
authors.lastname: Malinka
mesh_terms: D003300:Copper / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000138:chemical synthesis*; D011199:Potentiometry; D021241:Spectrometry, Mass, Electrospray Ionization; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D010455:Peptides; D015091:beta-Alanine; D003300:Copper; C011757:2,3-diaminopropionic acid