title: Genome-wide analysis of DNA methylation patterns in horse.
abstract: BACKGROUND
  DNA methylation is an epigenetic regulatory mechanism that plays an essential role in mediating biological processes and determining phenotypic plasticity in organisms. Although the horse reference genome and whole transcriptome data are publically available the global DNA methylation data are yet to be known.
  
  
  RESULTS
  We report the first genome-wide DNA methylation characteristics data from skeletal muscle, heart, lung, and cerebrum tissues of thoroughbred (TH) and Jeju (JH) horses, an indigenous Korea breed, respectively by methyl-DNA immunoprecipitation sequencing. The analysis of the DNA methylation patterns indicated that the average methylation density was the lowest in the promoter region, while the density in the coding DNA sequence region was the highest. Among repeat elements, a relatively high density of methylation was observed in long interspersed nuclear elements compared to short interspersed nuclear elements or long terminal repeat elements. We also successfully identified differential methylated regions through a comparative analysis of corresponding tissues from TH and JH, indicating that the gene body regions showed a high methylation density.
  
  
  CONCLUSIONS
  We provide report the first DNA methylation landscape and differentially methylated genomic regions (DMRs) of thoroughbred and Jeju horses, providing comprehensive DMRs maps of the DNA methylome. These data are invaluable resource to better understanding of epigenetics in the horse providing information for the further biological function analyses.
authors.forename: Ja-Rang
authors.initials: JR
authors.lastname: Lee
authors.forename: Chang Pyo
authors.initials: CP
authors.lastname: Hong
authors.forename: Jae-Woo
authors.initials: JW
authors.lastname: Moon
authors.forename: Yi-Deun
authors.initials: YD
authors.lastname: Jung
authors.forename: Dae-Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Tae-Hyung
authors.initials: TH
authors.lastname: Kim
authors.forename: Jeong-An
authors.initials: JA
authors.lastname: Gim
authors.forename: Jin-Han
authors.initials: JH
authors.lastname: Bae
authors.forename: Yuri
authors.initials: Y
authors.lastname: Choi
authors.forename: Jungwoo
authors.initials: J
authors.lastname: Eo
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Kwon
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Song
authors.forename: Junsu
authors.initials: J
authors.lastname: Ko
authors.forename: Young Mok
authors.initials: YM
authors.lastname: Yang
authors.forename: Hak-Kyo
authors.initials: HK
authors.lastname: Lee
authors.forename: Kyung-Do
authors.initials: KD
authors.lastname: Park
authors.forename: Kung
authors.initials: K
authors.lastname: Ahn
authors.forename: Kyoung-Tag
authors.initials: KT
authors.lastname: Do
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Ha
authors.forename: Kyudong
authors.initials: K
authors.lastname: Han
authors.forename: Joo Mi
authors.initials: JM
authors.lastname: Yi
authors.forename: Hee-Jae
authors.initials: HJ
authors.lastname: Cha
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Cho
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea. jongbhak@genomics.org.
authors.forename: Jong
authors.initials: J
authors.lastname: Bhak
authors.forename: Heui-Soo
authors.initials: HS
authors.lastname: Kim
mesh_terms: D000818:Animals; D054022:Cerebrum / Q000378:metabolism; D019295:Computational Biology; D018899:CpG Islands; D004247:DNA / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D016678:Genome; D006736:Horses / Q000235:genetics*; D008168:Lung / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA