title: An efficient preparative procedure for main flavonoids from the peel of Trichosanthes kirilowii Maxim. using polyamide resin followed by semi-preparative high performance liquid chromatography.
abstract: In this study, a simple and efficient preparative procedure was developed for preparation of seven flavonoids from the peel of Trichosanthes kirilowii Maxim. using polyamide resin followed by semi-preparative high performance liquid chromatography (SPHPLC). First, the ethyl acetate fraction from the peel of T. kirilowii Maxim. obtained "prefractionation" using polyamide resin, which yielded two subfractions. And then the two subfractions were isolated by SPHPLC with an isocratic elution of methanol-water. Finally, seven known flavonoids were purified from 35 g of ethyl acetate extract including quercetin-3-O-[α-l-rhamnose (1→2)-β-d-glucopyranosyl]-5-O-β-d-glucopyranoside (19 mg), quercetin-3-O-rutinoside (24 mg), apigenin-7-O-β-d-glucopyranoside (10mg), diosmetin-7-O-β-d-glucopyranoside (45 mg), luteolin (21 mg), apigenin (15 mg), and diosmetin (56 mg). The purities of the compounds were determined by HPLC and the chemical structures were confirmed by UV and NMR analysis. In the present study, a simple, effective, and rapid procedure was established for preparative separation of multiple components from the peel of T. kirilowii Maxim. Furthermore, it was scalable and economical, so it was a promising basis for large-scale preparation of flavonoids from other plant extracts.
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, PR China; College of Pharmaceutical Science, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China. Electronic address: lxpsdta2001@sina.com.
authors.forename: Aifeng
authors.initials: A
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, PR China.
authors.forename: Ailing
authors.initials: A
authors.lastname: Sun
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, PR China.
authors.forename: Renmin
authors.initials: R
authors.lastname: Liu
authors.affiliation: College of Pharmaceutical Science, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, PR China.
authors.forename: Jichun
authors.initials: J
authors.lastname: Cui
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D029778:Trichosanthes / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D009757:Nylons; D010936:Plant Extracts
keywords: Flavonoids; Polyamide resin; Semi-preparative high performance liquid chromatography; T. kirilowii Maxim.