title: [The use of real time PCR for quantitative determination of some propionic bacteria residing on human skin].
abstract: A test system has been developed for determination of propionic bacterial species residing on human skin. This system developed in the real time PCR format is applicable for quantitative determination and also detection of genomes of the following Propionibacterium species: P. acnes, P. granulosum and P. avidum. This system was used for analysis of wash samples from the skin of 17 pentathlon sportsmen and 16 students. All three species of propionic bacteria were found in all skin wash samples. However, contamination with P. acnes was two times higher in control group than in the group of pentathlon sportsmen.
authors.forename: A G
authors.initials: AG
authors.lastname: Globa
authors.forename: Ia I
authors.initials: IaI
authors.lastname: Alekseev
authors.forename: V G
authors.initials: VG
authors.lastname: Arzumanian
authors.forename: V A
authors.initials: VA
authors.lastname: Zaborova
authors.forename: A A
authors.initials: AA
authors.lastname: Guridova
mesh_terms: D000328:Adult; D056352:Athletes; D015373:Bacterial Typing Techniques; D017931:DNA Primers / Q000737:chemistry*; D006801:Humans; D008297:Male; D011424:Propionibacterium / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D011425:Propionibacterium acnes / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S
keywords: microbiota; polymerase chain reaction; propionic bacteria