title: Role of the nucleocapsid region in HIV-1 Gag assembly as investigated by quantitative fluorescence-based microscopy.
abstract: The Gag precursor of HIV-1, formed of the four proteic regions matrix (MA), capsid (CA), nucleocapsid (NC) and p6, orchestrates virus morphogenesis. This complex process relies on three major interactions, NC-RNA acting as a scaffold, CA-CA and MA-membrane that targets assembly to the plasma membrane (PM). The characterization of the molecular mechanism of retroviral assembly has extensively benefited from biochemical studies and more recently an important step forward was achieved with the use of fluorescence-based techniques and fluorescently labeled viral proteins. In this review, we summarize the findings obtained with such techniques, notably quantitative-based approaches, which highlight the role of the NC region in Gag assembly.
authors.affiliation: UMR 7213 CNRS, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch, France. Electronic address: hderocquigny@unistra.fr.
authors.forename: Hugues
authors.initials: H
authors.lastname: de Rocquigny
authors.affiliation: UMR 7213 CNRS, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch, France.
authors.forename: Salah Edin
authors.initials: SE
authors.lastname: El Meshri
authors.affiliation: UMR 7213 CNRS, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Richert
authors.affiliation: UMR 7213 CNRS, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Didier
authors.affiliation: UMR 7213 CNRS, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Darlix
authors.affiliation: UMR 7213 CNRS, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, 74 route du Rhin, F-67401 Illkirch, France. Electronic address: yves.mely@unistra.fr.
authors.forename: Yves
authors.initials: Y
authors.lastname: Mély
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008856:Microscopy, Fluorescence; D019251:Nucleocapsid / Q000737:chemistry / Q000378:metabolism*; D019590:Nucleocapsid Proteins / Q000378:metabolism; D011485:Protein Binding; D055503:Protein Multimerization; D021381:Protein Transport; D052736:Rous sarcoma virus / Q000502:physiology; D019065:Virus Assembly; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000378:metabolism*
chemical_list: D019590:Nucleocapsid Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
keywords: Assembly; FRET-FLIM; Fluorescence; Gag; HIV; Microscopy; NC