title: Inhibition of peripheral nociceptors by aminoglycosides produces analgesia in inflammatory pain models in the rat.
abstract: Aminoglycosides (AGs) modulate nociceptors and ionic channels expressed in sensory neurons. The AG applied in situ could be useful to alleviate hyperalgesia in animal models of inflammatory pain. We tested streptomycin (ST) and neomycin (NEO) as analgesic agents applied in situ in rat paw inflammation caused by formalin or carrageenan administration. The action of ST and NEO on the action potential discharge produced by acidic stimuli in isolated dorsal root ganglion neurons was also studied in current-clamp recordings. In the formalin test, ST and NEO significantly reduced the nociceptive behaviour. ST reduced the N-(4-methyl-2-quinazolinyl)-guanidine (GMQ)-induced nociceptive behaviour, and NEO diminished the hyperalgesia to thermonociception and mechanonociception produced by CAR. In the current-clamp experiments, ST and NEO reduced the generation of action potentials when an acidic solution was applied. We conclude that ST and NEO produce analgesia to inflammatory pain, an effect that is due in part to the inhibition of ASIC activation in sensory neurons.
authors.affiliation: Laboratorio de Fisiología Celular, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, México, D.F., México.
authors.forename: Francisco
authors.initials: F
authors.lastname: Mercado
authors.forename: Angélica
authors.initials: A
authors.lastname: Almanza
authors.forename: Karina
authors.initials: K
authors.lastname: Simón-Arceo
authors.forename: Omar
authors.initials: O
authors.lastname: López
authors.forename: Rosario
authors.initials: R
authors.lastname: Vega
authors.forename: Ulises
authors.initials: U
authors.lastname: Coffeen
authors.forename: Bernardo
authors.initials: B
authors.lastname: Contreras
authors.forename: Enrique
authors.initials: E
authors.lastname: Soto
authors.forename: Francisco
authors.initials: F
authors.lastname: Pellicer
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology / Q000627:therapeutic use*; D000698:Analgesia / Q000379:methods*; D000818:Animals; D004195:Disease Models, Animal; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D008297:Male; D009619:Nociceptors / Q000187:drug effects* / Q000473:pathology; D010146:Pain / Q000188:drug therapy* / Q000473:pathology; D010147:Pain Measurement / Q000187:drug effects* / Q000379:methods; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000617:Aminoglycosides