title: Antigen unmasking induced by superheating.
abstract: This paper is dedicated to the description of superheating as a method for antigen retrieval. In our investigation, this antigen retrieval method was used on thermal plate, heating tissue sections at temperature 120° C for 90 minutes. In the research we conducted the superheating method was applied to formalin-fixed paraffin-embedded tissue sections of breast tumors. The following monoclonal antibodies were used: estrogen receptor, progesterone receptor, epithelial membrane antigen, CD34, and Ki-67. With these tested antibodies we had good staining and no loss of tissue sections during the staining process.
authors.affiliation: Faculty of Medicine, University of Nis, Nis, Serbia.
authors.forename: Marin
authors.initials: M
authors.lastname: Basic
authors.forename: Maja
authors.initials: M
authors.lastname: Jovicic Milentijevic
authors.forename: David
authors.initials: D
authors.lastname: Basic
authors.forename: Hakija
authors.initials: H
authors.lastname: Basic
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D018952:Antigens, CD34 / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D001943:Breast Neoplasms / Q000175:diagnosis*; D005260:Female; D005557:Formaldehyde / Q000737:chemistry; D006361:Heating; D006801:Humans; D019394:Ki-67 Antigen / Q000276:immunology; D018396:Mucin-1 / Q000276:immunology; D016612:Paraffin Embedding; D011960:Receptors, Estrogen / Q000276:immunology; D011980:Receptors, Progesterone / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D000951:Antigens, Neoplasm; D019394:Ki-67 Antigen; D018396:Mucin-1; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D005557:Formaldehyde