title: Comparison of N-methyl-D-aspartate receptor subunit 1 and 4-hydroxynonenal in the hippocampus of natural and chemical-induced aging accelerated mice.
abstract: In this study, we compared N-methyl-D-aspartate receptor type 1 (NMDAR1) and 4-hydroxynonenal (4-HNE) in the hippocampus of D-galactose (D-gal)-induced and naturally aging models of mice. These markers represent general phenotypes in aging, and they allowed us to examine the possibility of D-gal as a chemical model agent for aging. We observed an age-dependent reduction of NMDAR1 and an increase in 4-HNE in the dentate gyrus, CA1, and CA3 regions of the hippocampus via immunohistochemistry and western blot analyses. In the D-gal-induced chemical aging model, we observed similar changes in NMDAR1 and 4-HNE although the degree of reduction/increase in NMDAR1/4-HNE was not as severe as that in the naturally aged mice. These results suggest that the D-gal-induced aging model is comparable to naturally aged mice and may be useful for studies of the aging hippocampus.
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Nam
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Chung
authors.forename: Jong Whi
authors.initials: JW
authors.lastname: Kim
authors.forename: Hyo Young
authors.initials: HY
authors.lastname: Jung
authors.forename: Hee Seon
authors.initials: HS
authors.lastname: Yim
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Yoo
authors.forename: Hajin
authors.initials: H
authors.lastname: Nam
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Jun-Gyo
authors.initials: JG
authors.lastname: Suh
authors.forename: Yeo Sung
authors.initials: YS
authors.lastname: Yoon
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D016194:Receptors, N-Methyl-D-Aspartate / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000447:Aldehydes; D016194:Receptors, N-Methyl-D-Aspartate; C027576:4-hydroxy-2-nonenal