title: Identification of T cell receptor signaling pathway proteins in a feline large granular lymphoma cell line by liquid chromatography tandem mass spectrometry.
abstract: Tryptic peptides of a feline large granular lymphoma cell line were analyzed by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Seventeen proteins of the T cell receptor signaling pathway could be identified by this approach. So far the existence of these proteins has only been postulated in the protein databases while experimental proof of their expression is predominantly pending. This article suggests where these proteins are located within the T cell receptor signaling pathway, thereby giving a short overview of the structure and function of this cascade.
authors.affiliation: Institute of Veterinary Pathology, Justus-Liebig University Giessen, Frankfurter Strasse 96, 35392 Giessen, Germany. Electronic address: Manfred.Henrich@vetmed.uni-giessen.de.
authors.forename: Manfred
authors.initials: M
authors.lastname: Henrich
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Justus-Liebig University Giessen, Schubertstrasse 60, Building 16, 35392 Giessen, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Huber
authors.affiliation: Institute of Veterinary Pathology, Justus-Liebig University Giessen, Frankfurter Strasse 96, 35392 Giessen, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Rydzewski
authors.affiliation: Institute of Veterinary Pathology, Justus-Liebig University Giessen, Frankfurter Strasse 96, 35392 Giessen, Germany.
authors.forename: Svenja
authors.initials: S
authors.lastname: Kirsten
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Justus-Liebig University Giessen, Schubertstrasse 60, Building 16, 35392 Giessen, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Spengler
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Justus-Liebig University Giessen, Schubertstrasse 60, Building 16, 35392 Giessen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Römpp
authors.affiliation: Institute of Veterinary Pathology, Justus-Liebig University Giessen, Frankfurter Strasse 96, 35392 Giessen, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Reinacher
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000378:metabolism*; D002415:Cats; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D008223:Lymphoma / Q000378:metabolism / Q000662:veterinary*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D053719:Tandem Mass Spectrometry
chemical_list: D011948:Receptors, Antigen, T-Cell
keywords: Cat; Cell line; LC–ESI–MS/MS; Large granular lymphoma; Mass spectrometry; T cell receptor signaling proteins