title: In vitro photodynamic inactivation of Sporothrix schenckii complex species.
abstract: Photodynamic therapy has been applied successfully against cutaneous and subcutaneous mycoses. We applied methylene blue as a photosensitizing agent and light emitting diode (InGaAlP) against Sporothrix schenckii complex species in an in vitro assay. The viability of the conidia was determined by counting colony-forming units. Methylene blue in conjunction with laser irradiation was able to inhibit the growth of all tested samples. The in vitro inhibition of Sporothrix spp. isolates by laser light deserves in vivo experimental and clinical studies since it may be a promising treatment for cutaneous and subcutaneous sporotrichosis.
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil debora_alves6@hotmail.com.
authors.forename: Débora Alves
authors.initials: DA
authors.lastname: Nunes Mario
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Laura Bedin
authors.initials: LB
authors.lastname: Denardi
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
authors.forename: Daniela Isabel
authors.initials: DI
authors.lastname: Brayer Pereira
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Janio Morais
authors.initials: JM
authors.lastname: Santurio
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
mesh_terms: D015169:Colony Count, Microbial; D004203:Disinfection / Q000379:methods*; D006801:Humans; D007834:Lasers; D008751:Methylene Blue / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects* / Q000528:radiation effects*; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000378:metabolism*; D013172:Spores, Fungal / Q000187:drug effects / Q000528:radiation effects; D013175:Sporothrix / Q000187:drug effects* / Q000528:radiation effects*
chemical_list: D017319:Photosensitizing Agents; D008751:Methylene Blue
keywords: PDT; laser; methylene blue; mycosis; sporotrichosis