title: The use of aloe vera extract as a novel storage media for the avulsed tooth.
abstract: BACKGROUND
  Tooth avulsion is one of the most severe dental traumas which most often occur in children. When immediate replantation is not possible, storage in a proper media may lead to a prolonged survival rate. Aloe Vera is a cactus like plant with green, tapered leaves that are filled with a transparent viscous gel. This medicinal plant has significant anti-inflammatory, antioxidant, antibacterial and antifungal effects. The purpose of this study was to assess the effectiveness of different concentrations of Aloe Vera extract compared to DMEM (cell culture medium) and egg white.
  
  
  METHODS
  The periodontal ligament (PDL) cells were cultured and certain number of cells were treated with Aloe Vera extract (in four different concentrations), egg white and culture media for 1, 3, 6, and 9 hours. Cell viability was determined by using the (3-[4, 5-dimethylthiazolyl-2]-2, 5-diphenyltetrazolium bromide) assay. Moreover, One-way ANOVA and post hoc (LSD) test were used for analyzing the study groups.
  
  
  RESULTS
  The results indicate that culture media and Aloe Vera extract (10, 30, and 50% concentration) were statistically similar and significantly preserved more PDL cells compared to other experimental storage media.
  
  
  CONCLUSIONS
  Aloe Vera 10, 30, and 50% may be recommended as a suitable storage media for avulsed teeth.
authors.affiliation: Department of Pediatric Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran;
authors.forename: Samaneh
authors.initials: S
authors.lastname: Badakhsh
authors.affiliation: Department of Pediatric Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran;
authors.forename: Tahereh
authors.initials: T
authors.lastname: Eskandarian
authors.affiliation: Laboratory of Stem Cell Research, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Esmaeilpour
keywords: Aloe Vera; Avulsed tooth; Cell viability; Egg white; Periodontal ligament