title: Localization of prestin and expression in the early period after radiation in mice.
abstract: This study aimed to examine expression and microstructural distribution of prestin in outer hair cells, and the effect of dose and time of radiation on prestin expression in the BALB/c mouse. We also investigated the molecular biological characteristics of prestin and possible mechanisms of sensorineural hearing loss caused by radiation. Seventy 4-week-old mice were randomly divided into four groups, including one control group and three experimental groups. Each experimental group was randomly divided into two groups, which were killed to collect specimens of the cochlea on the 3rd and 7th days after exposure to different doses of 8, 12, and 16 Gy radiation. These cochleas were embedded in paraffin, and then cut into sections. The sections were immunostained with anti-prestin antibodies. The distribution of prestin was observed under optical microscopy and the density of prestin-positive expression was quantitatively calculated by Image-Pro Plus. Prestin had high expression in the lateral membrane and low expression in the cytoplasm of outer hair cells above the nucleus. The density of prestin protein expression of the basal turn was not significantly different after exposure to the different doses of radiation compared with the control group, but up-regulation occurred after radiation in the apex turn. We conclude that prestin protein is mainly expressed in the lateral membrane above the nucleus. Prestin protein may be responsible for the mechanism of injury to the inner ear caused by radiation.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Nan Fang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Yuan
authors.forename: Chen
authors.initials: C
authors.lastname: Ren
authors.forename: Jin-Hao
authors.initials: JH
authors.lastname: Peng
mesh_terms: D000818:Animals; D005500:Follow-Up Studies; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism* / Q000528:radiation effects; D006319:Hearing Loss, Sensorineural / Q000209:etiology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020409:Molecular Motor Proteins / Q000096:biosynthesis*; D011833:Radiation Injuries, Experimental / Q000150:complications / Q000378:metabolism*; D013997:Time Factors
chemical_list: D020409:Molecular Motor Proteins; C498720:Pres protein, mouse