title: On-chip monitoring of skeletal myoblast transplantation for the treatment of hypoxia-induced myocardial injury.
abstract: A comprehensive elucidation of the unexpected adverse events that occur in skeletal myoblast transplantation is fundamental for the optimization of myocardial therapeutic effects. However, a well-defined method to study the interactions between skeletal myoblasts and cardiomyocytes during the healing process is out of reach. Here, we describe a microfluidic method for monitoring the interactions between skeletal myoblasts and hypoxia-injured cardiomyocytes in a spatiotemporally-controlled manner, mimicking the in vivo cell transplantation process. A myocardial hypoxia environment was created using an oxygen consumption blocking reagent, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone. Meanwhile, the interactions between the skeletal L6 myoblasts and hypoxia-injured myocardium H9c2 cells were investigated, and the effects of a L6 conditional medium on H9c2 cells were comparatively analyzed by quantitatively measuring the morphological and pathophysiological dynamics of H9c2 cells. The results showed that skeletal myoblasts could repair hypoxia-injured H9c2 cells mainly through direct cell-to-cell interactions. This simple on-chip assay for investigating myocardial repair processes may provide avenues for the in vitro screening of drug-induced cardiotoxicity.
authors.affiliation: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China. jywang@nwsuaf.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: He
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Wenming
authors.initials: W
authors.lastname: Liu
authors.forename: Jinyi
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002450:Cell Communication; D002460:Cell Line; D004867:Equipment Design; D000860:Hypoxia / Q000150:complications*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000637:transplantation*; D017202:Myocardial Ischemia / Q000209:etiology* / Q000473:pathology / Q000628:therapy*; D009206:Myocardium; D032383:Myocytes, Cardiac / Q000166:cytology / Q000473:pathology*; D051381:Rats