title: Surface modification of solid-state nanopores for sticky-free translocation of single-stranded DNA.
abstract: Nanopore technology is one of the most promising approaches for fast and low-cost DNA sequencing application. Single-stranded DNA detection is primary objective in such device, while solid-state nanopores remain less explored than their biological counterparts due to bio-molecule clogging issue caused by surface interaction between DNA and nanopore wall. By surface coating a layer of polyethylene glycol (PEG), solid-state nanopore can achieve long lifetime for single-stranded DNA sticky-free translocation at pH 11.5. Associated with elimination of non-specific binding of molecule, PEG coated nanopore presents new surface characteristic as less hydrophility, lower 1/f noise, and passivated surface charge responsiveness on pH. Meanwhile, conductance blockage of single-stranded DNA is found to be deeper than double-stranded DNA, which can be well described by a string of blobs model for a quasi-equilibrium state polymer in constraint space.
authors.affiliation: State Key Laboratory for Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, P. R. China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Tang
authors.forename: Bo
authors.initials: B
authors.lastname: Lu
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jiajun
authors.initials: J
authors.lastname: Wang
authors.forename: Kaifu
authors.initials: K
authors.lastname: Luo
authors.forename: Dapeng
authors.initials: D
authors.lastname: Yu
mesh_terms: D000268:Adhesiveness / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000737:chemistry* / Q000187:drug effects; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods; D009068:Movement / Q000187:drug effects; D058608:Nanopores; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D004277:DNA, Single-Stranded; D011092:Polyethylene Glycols
keywords: DNA sequencing; single-stranded DNA detection; solid-state nanopores; string of blobs; surface modification