title: Production of P-aminobenzoic acid by metabolically engineered escherichia coli.
abstract: The production of chemical compounds from renewable resources is an important issue in building a sustainable society. In this study, Escherichia coli was metabolically engineered by introducing T7lac promoter-controlled aroF(fbr), pabA, pabB, and pabC genes into the chromosome to overproduce para-aminobenzoic acid (PABA) from glucose. Elevating the copy number of chromosomal PT7lac-pabA-pabB distinctly increased the PABA titer, indicating that elevation of 4-amino-4-deoxychorismic acid synthesis is a significant factor in PABA production. The introduction of a counterpart derived from Corynebacterium efficiens, pabAB (ce), encoding a fused PabA and PabB protein, resulted in a considerable increase in the PABA titer. The introduction of more than two copies of PT7lac-pabAB (ce-mod), a codon-optimized pabAB (ce), into the chromosome of a strain that simultaneously overexpressed aroF(fbr) and pabC resulted in 5.1 mM PABA from 55.6 mM glucose (yield 9.2%). The generated strain produced 35 mM (4.8 g L(-1)) PABA from 167 mM glucose (yield 21.0%) in fed-batch culture.
authors.affiliation: a Osaka Municipal Technical Research Institute , Osaka , Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Koma
authors.forename: Hayato
authors.initials: H
authors.lastname: Yamanaka
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Moriyoshi
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Sakai
authors.forename: Takaya
authors.initials: T
authors.lastname: Masuda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sato
authors.forename: Kozo
authors.initials: K
authors.lastname: Toida
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohmoto
mesh_terms: D010129:4-Aminobenzoic Acid / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D060847:Metabolic Engineering; D011401:Promoter Regions, Genetic / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D005947:Glucose; D010129:4-Aminobenzoic Acid
keywords: Escherichia coli; aromatic compound; metabolic engineering; para-aminobenzoic acid