title: Poly(urethane-dimethylsiloxane) copolymers displaying a range of soft segment contents, noncytotoxic chemistry, and nonadherent properties toward endothelial cells.
abstract: Polyurethane copolymers based on α,ω-dihydroxypropyl poly(dimethylsiloxane) (PDMS) with a range of soft segment contents were prepared by two-stage polymerization, and their microstructures, thermal, thermomechanical, and surface properties, as well as in vitro hemo- and cytocompatibility were evaluated. All utilized characterization methods confirmed the existence of moderately microphase separated structures with the appearance of some microphase mixing between segments as the PDMS (i.e., soft segment) content increased. Copolymers showed higher crystallinity, storage moduli, surface roughness, and surface free energy, but less hydrophobicity with decreasing PDMS content. Biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact, an extraction method and after pretreatment of copolymers with multicomponent protein mixture, as well as by a competitive protein adsorption assay. Copolymers showed no toxic effect to endothelial cells and all copolymers, except that with the lowest PDMS content, exhibited resistance to endothelial cell adhesion, suggesting their unsuitability for long-term biomedical devices which particularly require re-endothelialization. All copolymers exhibited excellent resistance to fibrinogen adsorption and adsorbed more albumin than fibrinogen in the competitive adsorption assay, suggesting their good hemocompatibility. The noncytotoxic chemistry of these synthesized materials, combined with their nonadherent properties which are inhospitable to cell attachment and growth, underlie the need for further investigations to clarify their potential for use in short-term biomedical devices.
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000, Belgrade, Serbia.
authors.forename: Ivan S
authors.initials: IS
authors.lastname: Stefanović
authors.forename: Jasna
authors.initials: J
authors.lastname: Djonlagić
authors.forename: Gordana
authors.initials: G
authors.lastname: Tovilović
authors.forename: Jelena
authors.initials: J
authors.lastname: Nestorov
authors.forename: Vesna V
authors.initials: VV
authors.lastname: Antić
authors.forename: Sanja
authors.initials: S
authors.lastname: Ostojić
authors.forename: Marija V
authors.initials: MV
authors.lastname: Pergal
mesh_terms: D000327:Adsorption; D000818:Animals; D002152:Calorimetry, Differential Scanning; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003460:Crystallization; D004129:Dimethylpolysiloxanes / Q000633:toxicity*; D020724:Elastomers / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D018625:Microscopy, Atomic Force; D058105:Polymerization; D011140:Polyurethanes / Q000737:chemistry / Q000633:toxicity; D011506:Proteins / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014867:Water
chemical_list: D004129:Dimethylpolysiloxanes; D020724:Elastomers; D011140:Polyurethanes; D011506:Proteins; D014867:Water; C013830:baysilon
keywords: biocompatibility; competitive protein adsorption; cytotoxicity; endothelial cells; poly(urethane-dimethylsiloxane)s