title: Construction of an infectious clone of simian foamy virus of Japanese macaque (SFVjm) and phylogenetic analyses of SFVjm isolates.
abstract: Foamy viruses belong to the genus Spumavirus of the family Retroviridae and have been isolated from many mammalian species. It was reported that simian foamy viruses (SFVs) have co-evolved with host species. In this study, we isolated four strains (WK1, WK2, AR1 and AR2) of SFV (named SFVjm) from Japanese macaques (Macaca fuscata) in main island Honshu of Japan. We constructed an infectious molecular clone of SFVjm strain WK1, termed pJM356. The virus derived from the clone replicated and induced syncytia in human (human embryonic kidney 293T cells), African green monkey (Vero cells) and mouse cell lines (Mus dunni tail fibroblast cells). Phylogenetic analysis also revealed that these four SFVjm strains formed two distinct SFVjm clusters. SFVjm strains WK1 and WK2 and SFV isolated from Taiwanese macaques (Macaca cyclopis) formed one cluster, whereas strains AR1 and AR2 formed the other cluster with SFV isolated from a rhesus macaque (Macaca mulatta).
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, Kyoto 606-8507, Japan; Laboratory of Virolution, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, Kyoto 606-8507, Japan.
authors.forename: Rokusuke
authors.initials: R
authors.lastname: Yoshikawa
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan. Electronic address: so.nakagawa@tokai-u.jp.
authors.forename: So
authors.initials: S
authors.lastname: Nakagawa
authors.affiliation: Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors.forename: Munehiro
authors.initials: M
authors.lastname: Okamoto
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, Kyoto 606-8507, Japan; Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, Kyoto 606-8507, Japan. Electronic address: takavet@gmail.com.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004272:DNA, Mitochondrial; D057809:HEK293 Cells / Q000821:virology; D006801:Humans; D007564:Japan; D008251:Macaca / Q000821:virology; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D017386:Sequence Homology, Amino Acid; D053150:Simian foamy virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D016092:Spumavirus / Q000235:genetics / Q000302:isolation & purification; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D014764:Viral Proteins
keywords: Foamy virus; Infectious molecular clone; Japanese macaque