title: Bioactive electrospun fish sarcoplasmic proteins as a drug delivery system.
abstract: Nano-microfibers were made from cod (Gadus morhua) sarcoplasmic proteins (FSP) (Mw<200kDa) using the electrospinning technique. The FSP fibers were studied by scanning electron microscopy, and the fiber morphology was found to be strongly dependent on FSP concentration. Interestingly, the FSP fibers were insoluble in water. However, when exposed to proteolytic enzymes, the fibers were degraded. The degradation products of the FSP fibers proved to be inhibitors of the diabetes-related enzyme DPP-IV. The FSP fibers may have biomedical applications, among others as a delivery system. To demonstrate this, a dipeptide (Ala-Trp) was encapsulated into the FSP fibers, and the release properties were investigated in gastric buffer and in intestinal buffer. The release profile showed an initial burst release, where 30% of the compound was released within the first minute, after which an additional 40% was released (still exponential) within the next 30min (gastric buffer) or 15min (intestinal buffer). The remaining 30% was not released in the timespan of the experiment.
authors.affiliation: National Food Institute, Technical University of Denmark, Søltofts Plads 227, 2800 Kongens Lyngby, Denmark. Electronic address: kaste@food.dtu.dk.
authors.forename: Karen
authors.initials: K
authors.lastname: Stephansen
authors.affiliation: National Food Institute, Technical University of Denmark, Søltofts Plads 227, 2800 Kongens Lyngby, Denmark.
authors.forename: Ioannis S
authors.initials: IS
authors.lastname: Chronakis
authors.affiliation: National Food Institute, Technical University of Denmark, Søltofts Plads 227, 2800 Kongens Lyngby, Denmark.
authors.forename: Flemming
authors.initials: F
authors.lastname: Jessen
mesh_terms: D000818:Animals; D016503:Drug Delivery Systems; D004591:Electrophoresis, Polyacrylamide Gel; D048389:Gadus morhua; D008855:Microscopy, Electron, Scanning; D011506:Proteins / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
chemical_list: D011506:Proteins
keywords: Bioactive; Biodegradable; DPP-IV inhibitors; Drug delivery; Electrospinning; Proteins