title: Purification and cDNA cloning of a lectin and a lectin-like protein from Apios americana Medikus tubers.
abstract: An Apios americana lectin (AAL) and a lectin-like protein (AALP) were purified from tubers by chromatography on Butyl-Cellulofine, ovomucoid-Cellulofine, and DEAE-Cellulofine columns. AAL showed strong hemagglutinating activity toward chicken and goose erythrocytes, but AALP showed no such activity toward any of the erythrocytes tested. The hemagglutinating activity of AAL was not inhibited by mono- or disaccharides, but was inhibited by glycoproteins, such as asialofetuin and ovomucoid, suggesting that AAL is an oligosaccharide-specific lectin. The cDNAs of AAL and AALP consist of 1,093 and 1,104 nucleotides and encode proteins of 302 and 274 amino acid residues, respectively. Both amino acid sequences showed high similarity to known legume lectins, and those of their amino acids involved in carbohydrate and metal binding were conserved.
authors.affiliation: a Laboratory of Food Molecular Functionality , College of Agriculture, Ibaraki University , Ibaraki , Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kouzuma
authors.forename: Satoshi
authors.initials: S
authors.lastname: Irie
authors.forename: Rikiya
authors.initials: R
authors.lastname: Yamazaki
authors.forename: Masami
authors.initials: M
authors.lastname: Yonekura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D007887:Fabaceae / Q000235:genetics*; D006023:Glycoproteins / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000494:pharmacology; D018514:Plant Structures / Q000235:genetics*; D017421:Sequence Analysis; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D018076:DNA, Complementary; D006023:Glycoproteins; D037102:Lectins; D010940:Plant Proteins; D000516:alpha-Amylases
keywords: Apios americana; cDNA cloning; lectin; purification