title: Oligonucleotide microarray analysis reveals dysregulation of energy-related metabolism in insulin-sensitive tissues of type 2 diabetes patients.
abstract: Impaired insulin action within skeletal muscle, adipose tissue, and the liver is an important characteristic of type 2 diabetes (T2D). In order to identify common underlying defects in insulin-sensitive tissues that may be involved in the pathogenesis of T2D, the gene expression profiles of skeletal muscle, visceral adipose tissue, and liver from autopsy donors with or without T2D were examined using oligonucleotide microarrays and quantitative reverse transcriptase-PCR. Compared with controls, 691 genes were commonly dysregulated in these three insulin-sensitive tissues of humans with T2D. These co-expressed genes were enriched within the mitochondrion, with suggested involvement in energy metabolic processes such as glycolysis and gluconeogenesis, fatty acid beta oxidative, tricarboxylic acid cycle, and electron transport. Genes related to energy metabolism were mostly downregulated in diabetic skeletal muscle and visceral adipose tissue, while they were upregulated in the diabetic liver. This observed dysregulation in energy-related metabolism may be the underlying factor leading to the molecular mechanisms responsible for the insulin resistance of patients with T2D.
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Jiaotong University, Shanghai Peoples Hospital Affiliated, Shanghai, China.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, Huashan Hospital, Institute of Endocrinology and Diabetology at Fudan University, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: X C
authors.initials: XC
authors.lastname: Wang
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Jiaotong University, Shanghai Peoples Hospital Affiliated, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Jiaotong University, Shanghai Peoples Hospital Affiliated, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Jiaotong University, Shanghai Peoples Hospital Affiliated, Shanghai, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Yao
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Jiaotong University, Shanghai Peoples Hospital Affiliated, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Jiaotong University, Shanghai Peoples Hospital Affiliated, Shanghai, China pyongde@hotmail.com.
authors.forename: Y D
authors.initials: YD
authors.lastname: Peng
authors.affiliation: Department of Endocrinology, Huashan Hospital, Institute of Endocrinology and Diabetology at Fudan University, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: R M
authors.initials: RM
authors.lastname: Hu
mesh_terms: D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000473:pathology; D004734:Energy Metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D007328:Insulin / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008928:Mitochondria / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D007328:Insulin