title: Serum paraoxonase 1 activity and oxidative stress in pediatric patients with pulmonary tuberculosis.
abstract: OBJECTIVE
  The aim of this study was to determine the oxidative stress and paraoxonase 1 (PON1) levels in children with pulmonary tuberculosis (TB) compared to healthy controls, and to examine the association of demographical with oxidative stress.
  
  
  METHODS
  Forty children diagnosed with pulmonary TB and 40 age- and gender-matched healthy controls were enrolled in the study. Serum total antioxidant status (TAS), total oxidant status (TOS) and PON1 levels were measured. The oxidative stress index (OSI) was calculated to indicate the degree of oxidative stress.
  
  
  RESULTS
  The TAS levels were lower (1.73 ± 0.5 vs. 2.54 ± 1.2 μmol Trolox Eq/l) while TOS levels were significantly higher (26.9 ± 14.4 vs. 13.4 ± 7.7 μmol H2O2 Eq/l) in the TB group than in the controls (p < 0.001). The OSI was significantly higher in the TB group than in the controls (21.2 ± 5.1 vs. 6.5 ± 4.9 units, p = 0.006). Serum PON1 levels were significantly lower in the TB group than in the controls (14.2 ± 13.2 vs. 28.4 ± 17.3 U/l, p < 0.001). The lower PON1 levels correlated with TAS and OSI levels but not with anthropometric parameters (r = 0.264, p = 0.018 and r = -0.255, p = 0.023, respectively).
  
  
  CONCLUSIONS
  The TOS and OSI levels were higher and the TAS and PON1 levels were lower in pediatric patients with pulmonary TB when compared to healthy controls. This indicates greater oxidative stress in the patients.
authors.affiliation: Department of Pediatrics, Bezmialem Vakif University, Istanbul, Turkey.
authors.forename: Emel
authors.initials: E
authors.lastname: Torun
authors.forename: Ahmet Hakan
authors.initials: AH
authors.lastname: Gedik
authors.forename: Erkan
authors.initials: E
authors.lastname: Cakir
authors.forename: Tarik
authors.initials: T
authors.lastname: Umutoglu
authors.forename: Ozlem
authors.initials: O
authors.lastname: Gok
authors.forename: Ulkan
authors.initials: U
authors.lastname: Kilic
mesh_terms: D000367:Age Factors; D043303:Aryldialkylphosphatase / Q000097:blood*; D015992:Body Mass Index; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000175:diagnosis / Q000201:enzymology*
chemical_list: D043303:Aryldialkylphosphatase; C468406:PON1 protein, human