title: Multifunctional inorganic nanocontainers for DNA and drug delivery into living cells.
abstract: The design and synthesis of multifunctional nanomaterials could lead to applications relevant for biomedicine. Manufacturing porous particles to make them able to carry bioactive molecules into living cells represents a substantial goal towards the development of powerful tools for nanomedicine. This work describes a first example of using zeolite-L crystals as multifunctional nanocontainers to simultaneously deliver DNA oligonucleotides and organic molecules into living cells. Multifunctional zeolite-L was prepared by filling the pore system with guest molecules, whilst DNA was adsorbed electrostatically on their surface. The release kinetics of DNA and of the guest molecules into living cells was studied to prove the multiple-drug-delivery ability of the system. The localization of all the components in different cellular compartments was followed. The presented system may be a prototype for the development of novel nanoparticles for drug delivery and gene therapy.
authors.affiliation: Institut de science et d'ingénierie supramoleculaire (ISIS), Université de Strasbourg, 8 alleé Gaspard Monge, 67000 Strasbourg (France), Fax: (+33) 3-68-85-52-42.
authors.forename: Henning
authors.initials: H
authors.lastname: Lülf
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bertucci
authors.forename: Dedy
authors.initials: D
authors.lastname: Septiadi
authors.forename: Roberto
authors.initials: R
authors.lastname: Corradini
authors.forename: Luisa
authors.initials: L
authors.lastname: De Cola
mesh_terms: D004247:DNA / Q000737:chemistry*; D016503:Drug Delivery Systems; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D004247:DNA
keywords: DNA; drug delivery; fluorescence; mesoporous materials; nanotechnology; zeolites