title: Cellulase-assisted extraction and antioxidant activity of the polysaccharides from garlic.
abstract: In the present study, the polysaccharides were prepared from garlic by using a cellulase-assisted extraction method and the antioxidant activity of garlic polysaccharides (GPs) was evaluated. To improve the yield of GPs, the influences of the several factors such as extraction time, temperature, pH, and cellulase amount on the extraction efficiency were studied. The optimal conditions for extraction of GPs were determined as follows: time, 80 min; temperature, 45 °C; pH, 5; cellulase amount, 8000 U/g. Under the optimised extraction conditions, the yield of GPS reached up to 35.34%. The GPs product exhibited strong antioxidant activity including hydroxyl radical scavenging activity, 2,2-diphenyl-β-picrylhydrazyl radical scavenging activity, and reducing power. The results suggest that the GPs could be used as potential antioxidants.
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China.
authors.forename: Saikun
authors.initials: S
authors.lastname: Pan
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China. Electronic address: wushengjun008@sina.com.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D002480:Cellulase / Q000378:metabolism; D005737:Garlic / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011134:Polysaccharides; D017665:Hydroxyl Radical; D002480:Cellulase
keywords: Antioxidant activity; Cellulase-assisted extraction; Polysaccharides