title: Lactobacillus paracasei CBA L74 interferes with gliadin peptides entrance in Caco-2 cells.
abstract: Several recent reports describe a role of probiotics as a therapeutic approach for celiac disease (CD). Two undigested A-gliadin peptides, P31-43 and P57-68, are central to CD pathogenesis, inducing an innate and an adaptive immune response, respectively. They enter enterocytes and localize to vesicular compartment to induce their toxic/immunogenics effects. In this article, we tested the effect of probiotic Lactobacillus paracasei (LP) CBA L74 (International Depository Accession Number LMG P-24778), its supernatant and LP-fermented cereals on gliadin peptides, P31-43 and P57-68, entrance in Caco-2 cells. Both LP CBA L74 and its supernatant inhibit P31-43 (intensity of fluorescence; FI: 75%) and P57-68 (FI: 50%) entrance in Caco2 cells, indicating that this biological effect is due to some product included in LP CBA L74 supernatant. This effect was present also after fermentation of cereals. This study describes a novel effect of probiotics in the prevention of undigested gliadin peptides toxic effects.
authors.affiliation: Department of Translational Medical Science and European Laboratory for the Investigation of Food Induced Disease (ELFID), University of Naples Federico II , Naples , Italy .
authors.forename: Marco
authors.initials: M
authors.lastname: Sarno
authors.forename: Giuliana
authors.initials: G
authors.lastname: Lania
authors.forename: Marialaura
authors.initials: M
authors.lastname: Cuomo
authors.forename: Federica
authors.initials: F
authors.lastname: Nigro
authors.forename: Francesca
authors.initials: F
authors.lastname: Passannanti
authors.forename: Andrea
authors.initials: A
authors.lastname: Budelli
authors.forename: Francesca
authors.initials: F
authors.lastname: Fasano
authors.forename: Riccardo
authors.initials: R
authors.lastname: Troncone
authors.forename: Salvatore
authors.initials: S
authors.lastname: Auricchio
authors.forename: Maria Vittoria
authors.initials: MV
authors.lastname: Barone
authors.forename: Roberto
authors.initials: R
authors.lastname: Nigro
authors.forename: Merlin
authors.initials: M
authors.lastname: Nanayakkara
mesh_terms: D001688:Biological Products / Q000494:pharmacology* / Q000627:therapeutic use; D018938:Caco-2 Cells; D002446:Celiac Disease / Q000188:drug therapy / Q000378:metabolism*; D002478:Cells, Cultured; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D002523:Edible Grain / Q000382:microbiology; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism; D005285:Fermentation; D005903:Gliadin / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007778:Lactobacillus; D010455:Peptides / Q000378:metabolism*; D019936:Probiotics / Q000627:therapeutic use
chemical_list: D001688:Biological Products; D010455:Peptides; D005903:Gliadin
keywords: Celiac disease; Lactobacillus paracasei CBA L74; fermented food; gliadin peptides; vesicular trafficking