title: Characterization and expression analysis of an interferon-γ2 induced chemokine receptor CXCR3 in common carp (Cyprinus carpio L.).
abstract: Chemokine and chemokine receptor signalling pairs play a crucial role in regulation of cell migration, morphogenesis, and cell activation. Expressed in mammals on activated T and NK cells, chemokine receptor CXCR3 binds interferon-γ inducible chemokines CXCL9-11 and CCL21. Here we sequenced the carp CXCR3 chemokine receptor and showed its relationship to CXCR3a receptors found in other teleosts. We found high expression of the CXCR3 gene in most of the organs and tissues of the immune system and in immune-related tissues such as gills and gut, corroborating a predominantly immune-related function. The very high expression in gill and gut moreover indicates a role for CXCR3 in cell recruitment during infection. High in vivo expression of CXCR3 at later stages of inflammation, as well as its in vitro sensitivity to IFN-γ2 stimulation indicate that in carp, CXCR3 is involved in macrophage-mediated responses. Moreover, as expression of the CXCR3 and CXCb genes coincides in the focus of inflammation and as both the CXCb chemokines and the CXCR3 receptor are significantly up-regulated upon IFN-γ stimulation it is hypothesized that CXCb chemokines may be putative ligands for CXCR3.
authors.affiliation: Department of Evolutionary Immunology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, PL30-387 Krakow, Poland. Electronic address: magdalena.chadzinska@uj.edu.pl.
authors.forename: M
authors.initials: M
authors.lastname: Chadzinska
authors.affiliation: Cell Biology and Immunology Group, Dept of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH Wageningen, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Golbach
authors.affiliation: Department of Evolutionary Immunology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, PL30-387 Krakow, Poland.
authors.forename: L
authors.initials: L
authors.lastname: Pijanowski
authors.affiliation: Cell Biology and Immunology Group, Dept of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH Wageningen, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Scheer
authors.affiliation: Cell Biology and Immunology Group, Dept of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH Wageningen, The Netherlands.
authors.forename: B M L
authors.initials: BM
authors.lastname: Verburg-van Kemenade
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D060226:Head Kidney / Q000166:cytology / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D008969:Molecular Sequence Data; D010802:Phylogeny; D054367:Receptors, CXCR3 / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D029941:Fish Proteins; D054367:Receptors, CXCR3; D007371:Interferon-gamma
keywords: CXCR3; Carp; Chemokine; Chemokine receptor; Interferon-γ; Macrophage