title: Microneedle enhanced delivery of cosmeceutically relevant peptides in human skin.
abstract: Peptides and proteins play an important role in skin health and well-being. They are also found to contribute to skin aging and melanogenesis. Microneedles have been shown to substantially enhance skin penetration and may offer an effective means of peptide delivery enhancement. The aim of this investigation was to assess the influence of microneedles on the skin penetration of peptides using fluorescence imaging to determine skin distribution. In particular the effect of peptide chain length (3, 4, 5 amino acid chain length) on passive and MN facilitated skin penetration was investigated. Confocal laser scanning microscopy was used to image fluorescence intensity and the area of penetration of fluorescently tagged peptides. Penetration studies were conducted on excised full thickness human skin in Franz type diffusion cells for 1 and 24 hours. A 2 to 22 fold signal improvement in microneedle enhanced delivery of melanostatin, rigin and pal-KTTKS was observed. To our knowledge this is the first description of microneedle enhanced skin permeation studies on these peptides.
authors.affiliation: Dermatology Research Centre, The University of Queensland, School of Medicine, Translational Research Institute, Brisbane, Queensland, Australia; School of Pharmacy, CHIRI-Biosciences, Curtin University, Perth, Western Australia, Australia; Therapeutics Research Centre, The University of Queensland, School of Medicine, Princess Alexandra Hospital, Brisbane, Queensland, Australia.
authors.forename: Yousuf H
authors.initials: YH
authors.lastname: Mohammed
authors.affiliation: Dermatology Research Centre, The University of Queensland, School of Medicine, Translational Research Institute, Brisbane, Queensland, Australia.
authors.forename: Miko
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Dermatology Research Centre, The University of Queensland, School of Medicine, Translational Research Institute, Brisbane, Queensland, Australia.
authors.forename: Lynlee L
authors.initials: LL
authors.lastname: Lin
authors.affiliation: Therapeutics Research Centre, The University of Queensland, School of Medicine, Princess Alexandra Hospital, Brisbane, Queensland, Australia.
authors.forename: Jeffrey E
authors.initials: JE
authors.lastname: Grice
authors.affiliation: Therapeutics Research Centre, The University of Queensland, School of Medicine, Princess Alexandra Hospital, Brisbane, Queensland, Australia.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Roberts
authors.affiliation: Dermatology Research Centre, The University of Queensland, School of Medicine, Translational Research Institute, Brisbane, Queensland, Australia.
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Raphael
authors.affiliation: School of Pharmacy, CHIRI-Biosciences, Curtin University, Perth, Western Australia, Australia.
authors.forename: Heather A E
authors.initials: HA
authors.lastname: Benson
authors.affiliation: Dermatology Research Centre, The University of Queensland, School of Medicine, Translational Research Institute, Brisbane, Queensland, Australia.
authors.forename: Tarl W
authors.initials: TW
authors.lastname: Prow
mesh_terms: D061645:Abdominoplasty; D000279:Administration, Cutaneous; D003357:Cosmetic Techniques; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D009075:MSH Release-Inhibiting Hormone / Q000008:administration & dosage; D008845:Microinjections / Q000379:methods*; D018613:Microscopy, Confocal; D009842:Oligopeptides / Q000008:administration & dosage; D061848:Optical Imaging; D010455:Peptides / Q000008:administration & dosage* / Q000627:therapeutic use; D012867:Skin; D012879:Skin Physiological Phenomena; D013518:Surgery, Plastic / Q000379:methods*
chemical_list: D009842:Oligopeptides; D010455:Peptides; D009075:MSH Release-Inhibiting Hormone; C541250:palmitoyl-lysyl-threonyl-threonyl-lysyl-serine; C031491:rigin