title: Antioxidant and anti-inflammatory active dihydrobenzofuran neolignans from the seeds of Prunus tomentosa.
abstract: Prunus tomentosa seeds were researched for antioxidant and anti-inflammatory constituents. By activity-guided fractionation of P. tomentosa seed extract, six new dihydrobenzofuran neolignans, prunustosanans AI-IV (1-4) and prunustosanansides AI and AII (5 and 6), together with 10 known compounds (7-16) were isolated from bioactive fraction. The structures were determined by spectroscopic analyses, especially NMR, HRESIMS, and CD spectra. The antioxidant activity was greatest for 5, 10, and 12 against DPPH radical and for 8, 9, and 13 against ABTS radical. Moreover, compounds 7 and 11 exhibited much stronger inhibitory activity on nitric oxide (NO) production in murine microglia BV-2 compared with positive control minocycline (IC50 = 19.7 ± 1.5 μM). The results show that P. tomentosa seeds can be regarded as a potential source of antioxidants and inflammation inhibitors.
authors.affiliation: School of Traditional Chinese Materia Medica, ‡Key Laboratory of Structure-Based Drug Design and Discovery (Ministry of Education), and #School of Pharmacy, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Qing-Bo
authors.initials: QB
authors.lastname: Liu
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Huang
authors.forename: Ming
authors.initials: M
authors.lastname: Bai
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Chang
authors.forename: Xin-Jia
authors.initials: XJ
authors.lastname: Yan
authors.forename: Tao
authors.initials: T
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Shao-Jiang
authors.initials: SJ
authors.lastname: Song
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis* / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001572:Benzofurans / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D017705:Lignans / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D017628:Microglia; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D027861:Prunus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001572:Benzofurans; D017705:Lignans; D010936:Plant Extracts