title: Horseradish peroxidase-catalyzed formation of hydrogels from chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups.
abstract: Horseradish peroxidase-catalyzed cross-linking was applied to prepare hydrogels from aqueous solutions containing chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups (denoted as Ph-chitosan and Ph-PVA, respectively). Comparing the hydrogels prepared from the solution of 1.0% (w/v) Ph-chitosan and 3.0% (w/v) Ph-PVA and that of 3.0% (w/v) Ph-chitosan and 1.0% (w/v) Ph-PVA, the gelation time of the former hydrogel was 47 s, while was 10s longer than that of the latter one. The breaking point for the former hydrogel under stretching (114% strain) was approximately twice larger than that for the latter one. The swelling ratio of the former hydrogel in saline was about half of the latter one. Fibroblastic cells did not adhere on the former hydrogel but adhered and spread on the latter one. The growth of Escherichia coli cells was fully suppressed on the latter hydrogel during 48 h cultivation.
authors.affiliation: Division of Chemical Engineering, Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan. Electronic address: sakai@cheng.es.osaka-u.ac.jp.
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.affiliation: Department of Chemical Engineering, Sahand University of Technology, Tabriz 51335-1996, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Khanmohammadi
authors.affiliation: Department of Chemical Engineering, Sahand University of Technology, Tabriz 51335-1996, Iran.
authors.forename: Ali Baradar
authors.initials: AB
authors.lastname: Khoshfetrat
authors.affiliation: Division of Chemical Engineering, Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000517:prevention & control; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011142:Polyvinyl Alcohol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D020100:Hydrogels; D010636:Phenols; D011142:Polyvinyl Alcohol; D048271:Chitosan; D006735:Horseradish Peroxidase
keywords: Antibacterial activity; Chitosan; Enzymatic cross-linking; Horseradish peroxidase; Hydrogel; Poly(vinyl alcohol)