title: Highly sensitive fluorescence and SERS detection of azide through a simple click reaction of 8-chloroquinoline and phenylacetylene.
abstract: In 0.19 mol/L acetic acid (HAc), a click reaction of 8-chloroquinoline/azide/phenylacetylene take places in aqueous solution without Cu(I) as a catalyst. 8-Chloroquinoline (CQN) exhibited a strong fluorescence peak at 430 nm that was quenched linearly as the concentration of azide increased from 20 to 1000 ng/mL. This quenching was due to consumption of CQN in the click reaction and a decrease in the number of efficiently excited photons due to the presence of triazole-quinoline ramification molecules with strong hydrophobicity. Using blue nanosilver sol as the substrate, CQN absorbed onto the surface of nanosilver particles, showing a strong surface-enhanced Raman scattering (SERS) peak at 1585 cm(-1) that decreased linearly as the azide concentration increased from 8 to 500 ng/mL; the detection limit was 4 ng/mL. Thus, two new, simple and sensitive fluorescence and SERS methods have been developed for the determination of azide via the click reaction.
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zeng
authors.forename: Lingling
authors.initials: L
authors.lastname: Ye
authors.forename: Lu
authors.initials: L
authors.lastname: Ma
authors.forename: Wenqing
authors.initials: W
authors.lastname: Yin
authors.forename: Tingsheng
authors.initials: T
authors.lastname: Li
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D000114:Acetylene / Q000031:analogs & derivatives* / Q000737:chemistry; D001386:Azides / Q000032:analysis* / Q000737:chemistry; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D005453:Fluorescence; D007834:Lasers; D057230:Limit of Detection; D011804:Quinolines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: C000607208:8-chloroquinoline; D001386:Azides; D011804:Quinolines; C044736:phenylacetylene; D012834:Silver; D003300:Copper; D000114:Acetylene
keywords: N3-; SERS; blue nanosilver sol; click reaction; fluorescence