title: A simple and rapid method for visualization of male meiotic chromosomes in Arabidopsis thaliana.
abstract: Meiotic chromosomes are of basic interest to the geneticist and cell biologist who study their behavior. A rapid and highly repeatable method for visualization of meiotic chromosomes is useful. Here we describe a fast staining protocol for Arabidopsis male meiotic chromosomes. Meiocytes were squashed into a labeling buffer, the chromosome morphology could be analyzed using fluorescence without any additional treatment.
authors.affiliation: a College of Life Sciences , Henan Normal University , Xinxiang , China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Li
authors.forename: Jinhong
authors.initials: J
authors.lastname: Yuan
authors.forename: Xiting
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.forename: Mingjun
authors.initials: M
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000166:cytology*; D032461:Chromosomes, Plant / Q000378:metabolism*; D008540:Meiosis; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
keywords: Arabidopsis; chromosomes; cytogenetics; meiosis; microscopy