title: Single-step approach for fabrication of vancomycin-bonded silica monolith as chiral stationary phase.
abstract: A vancomycin-bonded silica monolithic column for capillary electrochromatography (CEC) was prepared by a single-step in situ sol-gel approach. This sol-gel process incorporates a synthetic sol-gel precursor which contains a macrocyclic antibiotic, vancomycin, to form a porous silica network inside a fused-silica capillary. To avoid degradation of vancomycin during the column fabrication, a mild step was adopted into the sol-gel process. The performance of the vancomycin chiral stationary phase was investigated by CEC in both the reversed-phase mode and the normal-phase mode. The vancomycin chiral stationary phase was optimized with respect to vancomycin loading in the reversed-phase mode for chiral separation of thalidomide enantiomers. The best efficiency and resolution values of 94600plates/m and 5.79, respectively, were achieved. The optimized column was further applied to chiral separation of alprenolol enantiomers. A plate height of less than 7μm for the first eluted enantiomer of alprenolol was obtained in an aqueous mobile phase at a flow rate of 0.74mm/s. Using enantiomers of seven β-blockers and some other basic enantiomers as test analytes, separation efficiencies of up to 148100plates/m in the reversed-phase mode and up to 138100plates/m in the normal-phase mode were achieved.
authors.affiliation: Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Min-Hsiung, Chia-Yi 62102, Taiwan, ROC.
authors.forename: Ming-Lung
authors.initials: ML
authors.lastname: Hsieh
authors.affiliation: Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Min-Hsiung, Chia-Yi 62102, Taiwan, ROC; Center for Nano Bio-Detection and Advanced Institute of Manufacturing with High-tech Innovations, National Chung Cheng University, 168 University Road, Min-Hsiung, Chia-Yi 62102, Taiwan, ROC. Electronic address: chelkc@ccu.edu.tw.
authors.forename: Lai-Kwan
authors.initials: LK
authors.lastname: Chau
authors.affiliation: Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Min-Hsiung, Chia-Yi 62102, Taiwan, ROC.
authors.forename: Yung-Son
authors.initials: YS
authors.lastname: Hon
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000302:isolation & purification; D000526:Alprenolol / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D005782:Gels; D044367:Phase Transition; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D013499:Surface Properties; D013792:Thalidomide / Q000302:isolation & purification; D014640:Vancomycin / Q000737:chemistry*
chemical_list: D000319:Adrenergic beta-Antagonists; D005782:Gels; D013792:Thalidomide; D014640:Vancomycin; D012822:Silicon Dioxide; D000526:Alprenolol
keywords: Capillary electrochromatography; Chiral separation; Monolithic column; Sol–gel process; Vancomycin