title: [Study of codon 72 of p53 gene as a risk-factor in cervical cancer in Senegal].
abstract: Beside human papilloma virus infection, several genetic factors have been involved in susceptibility to cervical cancer. The arginine allele at codon 72 in p53 tumor suppressor gene has been reported to be a risk-factor in different ethnic groups. Our aim was to study this polymorphism as a risk-factor in Senegal. We conducted a case-control association study by recruiting 30 patients with cervical cancer clinically followed up in the Curie Institute in Dakar, and 93 healthy female controls without diagnosed cervical cancer. For each individual, DNA was extracted from whole blood. The codon 72 polymorphism was genotyped by PCR-RFLP. We did not find any association between the arginine allele and susceptibility to cervical cancer in our population (P = 0.354). Moreover, any correlation between the arginine allele and histological lesions was observed. Even if we did not find any correlation between the arginine allele and susceptibility to cervical cancer, p53 as a tumor suppressor gene remains a good genetic marker in tumours biology.
authors.affiliation: Hôpital Aristide-Le-Dantec, laboratoire de cytologie clinique, cytogénétique et biologie de la reproduction, Dakar, Sénégal, Université Cheikh-Anta-Diop, faculté de médecine, pharmacie et odontologie, laboratoire de biochimie pharmaceutique, Dakar, Sénégal.
authors.forename: Rokhaya
authors.initials: R
authors.lastname: Ndiaye
authors.affiliation: Institut Juliot-Curie, hôpital Aristide-Le-Dantec, Dakar, Sénégal.
authors.forename: Ahmadou
authors.initials: A
authors.lastname: Dem
authors.affiliation: Hôpital Aristide-Le-Dantec, laboratoire de cytologie clinique, cytogénétique et biologie de la reproduction, Dakar, Sénégal.
authors.forename: Pape Matar
authors.initials: PM
authors.lastname: Mbaye
authors.affiliation: Université Cheikh-Anta-Diop, faculté de médecine, pharmacie et odontologie, laboratoire de biochimie pharmaceutique, Dakar, Sénégal.
authors.forename: Papa Madièye
authors.initials: PM
authors.lastname: Guèye
authors.affiliation: Université Cheikh-Anta-Diop, faculté des sciences et techniques, Dakar, Sénégal.
authors.forename: Gora
authors.initials: G
authors.lastname: Diop
authors.affiliation: Université Cheikh-Anta-Diop, faculté de médecine, pharmacie et odontologie, laboratoire de biochimie pharmaceutique, Dakar, Sénégal.
authors.forename: Papa Amadou
authors.initials: PA
authors.lastname: Diop
authors.affiliation: Hôpital Aristide-Le-Dantec, laboratoire de cytologie clinique, cytogénétique et biologie de la reproduction, Dakar, Sénégal.
authors.forename: Oumar
authors.initials: O
authors.lastname: Faye
mesh_terms: D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001120:Arginine / Q000235:genetics*; D016022:Case-Control Studies; D003062:Codon; D005260:Female; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012675:Senegal; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D003062:Codon; D001120:Arginine
keywords: Senegal; cervical cancer; codon 72; genetic risk factor; p53