title: P22 mediated recombination of frt-sites.
abstract: Flp mediated site specific recombination of frt-sites is frequently used in genetic engineering to excise, insert or invert DNA-cassettes in the chromosome. While constructs flanked by frt-sites are generally considered to be stable in the absence of the Flp enzyme, we observed that P22 chromosomes exceeding wild-type length tend to lose frt-flanked insertions via Flp independent recombination of frt-sites during phage propagation. This spontaneous recombination should be considered when engineering the chromosome of P22 and perhaps of other phages as well.
authors.affiliation: Laboratory of Food Microbiology, Department of Microbial and Molecular Systems (M(2)S), Faculty of Bioscience Engineering, KU Leuven, Kasteelpark Arenberg 23, B-3001 Leuven, Belgium.
authors.forename: Angela
authors.initials: A
authors.lastname: Makumi
authors.affiliation: Laboratory of Food Microbiology, Department of Microbial and Molecular Systems (M(2)S), Faculty of Bioscience Engineering, KU Leuven, Kasteelpark Arenberg 23, B-3001 Leuven, Belgium.
authors.forename: William
authors.initials: W
authors.lastname: Cenens
authors.affiliation: Laboratory of Gene Technology, Department of Biosystems, Faculty of Bioscience Engineering, KU Leuven, Leuven, Belgium.
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
authors.affiliation: Laboratory of Food Microbiology, Department of Microbial and Molecular Systems (M(2)S), Faculty of Bioscience Engineering, KU Leuven, Kasteelpark Arenberg 23, B-3001 Leuven, Belgium. Electronic address: Abram.Aertsen@biw.kuleuven.be.
authors.forename: Abram
authors.initials: A
authors.lastname: Aertsen
mesh_terms: D017100:Bacteriophage P22 / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D004279:DNA, Viral
keywords: Bacteriophage P22; Recombination; Salmonella Typhimurium; frt-sites