title: A magnetic nanoparticle-based multiple-gene delivery system for transfection of porcine kidney cells.
abstract: Superparamagnetic nanoparticles are promising candidates for gene delivery into mammalian somatic cells and may be useful for reproductive cloning using the somatic cell nuclear transfer technique. However, limited investigations of their potential applications in animal genetics and breeding, particularly multiple-gene delivery by magnetofection, have been performed. Here, we developed a stable, targetable and convenient system for delivering multiple genes into the nuclei of porcine somatic cells using magnetic Fe3O4 nanoparticles as gene carriers. After surface modification by polyethylenimine, the spherical magnetic Fe3O4 nanoparticles showed strong binding affinity for DNA plasmids expressing the genes encoding a green (DNAGFP) or red (DNADsRed) fluorescent protein. At weight ratios of DNAGFP or DNADsRed to magnetic nanoparticles lower than or equal to 10∶1 or 5∶1, respectively, the DNA molecules were completely bound by the magnetic nanoparticles. Atomic force microscopy analyses confirmed binding of the spherical magnetic nanoparticles to stretched DNA strands up to several hundred nanometers in length. As a result, stable and efficient co-expression of GFP and DsRed in porcine kidney PK-15 cells was achieved by magnetofection. The results presented here demonstrate the potential application of magnetic nanoparticles as an attractive delivery system for animal genetics and breeding studies.
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Zhongguancun Haidian District, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Zhongguancun Haidian District, Beijing, China.
authors.forename: Haixin
authors.initials: H
authors.lastname: Cui
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Haidian District, Beijing, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Li
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Zhongguancun Haidian District, Beijing, China.
authors.forename: Changjiao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Zhongguancun Haidian District, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Zhongguancun Haidian District, Beijing, China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Cui
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Zhongguancun Haidian District, Beijing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Zhongguancun Haidian District, Beijing, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004247:DNA; D018014:Gene Transfer Techniques; D007668:Kidney; D058185:Magnetite Nanoparticles; D010957:Plasmids; D013552:Swine; D014162:Transfection / Q000379:methods*
chemical_list: D058185:Magnetite Nanoparticles; D004247:DNA