title: Azo-sulforhodamine dyes: a novel class of broad spectrum dark quenchers.
abstract: A rapid access to a novel class of water-soluble dark quencher dyes was achieved using an azo-coupling reaction between a fluorescent primary arylamine derived from a sulforhodamine 101 scaffold and a tertiary aniline equipped with different bioconjugatable groups. The thus obtained nonfluorescent azo-sulforhodamine hybrids display a broad quenching range spanning the visible to NIR regions. This was demonstrated through the preparation and enzymatic activation of FRET-based fluorogenic substrates of urokinase.
authors.affiliation: Normandie Université , COBRA UMR 6014 & FR 3038, UNIV Rouen, INSA Rouen, CNRS, IRCOF, 1 Rue Tesnières, 76821 Mont-Saint-Aignan Cedex, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Chevalier
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Renard
authors.forename: Anthony
authors.initials: A
authors.lastname: Romieu
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry*; D004396:Coloring Agents / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes; D015394:Molecular Structure; D012235:Rhodamines / Q000138:chemical synthesis* / Q000737:chemistry; D012995:Solubility; D013055:Spectrophotometry, Infrared; D014867:Water
chemical_list: D000814:Aniline Compounds; D001391:Azo Compounds; D004396:Coloring Agents; D005456:Fluorescent Dyes; D012235:Rhodamines; D014867:Water; C050829:sulforhodamine 101; C023650:aniline