title: Assessment of cytokine expression profile in acute myeloid leukemia patients before and after chemotherapy.
abstract: OBJECTIVE
  One of the major goals of cancer treatment is the monitoring of chemotherapeutic protocols. Quantitative and comparative cytokine expression profiling could be reliable to be used for biomarkers in deadly and fast-growing cancers such as acute myeloid leukemia (AML). The present study aims to assess and further validate cytokines with probable effects on proliferation and maturation of blood cells in AML.
  
  
  METHODS
  Gene expression levels of IL-1β, IL-10, IL-8, TNF-α, and IFN-γ were analyzed before and after chemotherapy and after granulocyte colony-stimulating factor (G-CSF) therapy in 46 AML patients by an in-house quantitative comparative RT-PCR method.
  
  
  RESULTS
  Our findings indicated that although the gene expression level of TNF-α was almost constant in all 3 samples, IL-1β, IL-8, and IL-10 expression levels showed a decrease after chemotherapy and an increase after G-CSF therapy. On the other hand, the expression level of IFN-γ had a different pattern with an increase after chemotherapy and a decrease after G-CSF therapy.
  
  
  CONCLUSIONS
  Taken together, the results of this study are in support of the idea that the analyzed cytokines could be useful biomarkers for AML treatment monitoring. However, further molecular epidemiological investigations are suggested to elaborate more cancer monitoring biomarkers.
authors.affiliation: Tehran University of Medical Sciences Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Tehran, Iran ; Tehran University of Medical Sciences, Biotechnology Research Center, Tehran, Iran.
authors.forename: Zargham
authors.initials: Z
authors.lastname: Sepehrizadeh
authors.affiliation: Tehran University of Medical Sciences, Biotechnology Research Center, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mohammadi
authors.affiliation: Tehran University of Medical Sciences Fa culty of Medicine, Department of Hematology and Oncology, Tehran, Iran.
authors.forename: Amirhossein
authors.initials: A
authors.lastname: Emami
authors.affiliation: Tehran University of Medical Sciences Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Tehran, Iran.
authors.forename: Mojtaba Tabatabaei
authors.initials: MT
authors.lastname: Yazdi
authors.affiliation: Tehran University of Medical Sciences Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Tehran, Iran.
authors.forename: Saeed Hashemi
authors.initials: SH
authors.lastname: Bozchlou
authors.affiliation: Tehran University of Medical Sciences, Biotechnology Research Center, Tehran, Iran ; Tehran University of Medical Sciences Faculty of Public Health, Department of Pathobiology, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Khorramizadeh
authors.affiliation: Tehran University of Medical Sciences Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Tehran, Iran.
authors.forename: Mina Bahrololoumi
authors.initials: MB
authors.lastname: Shapourabadi
authors.affiliation: Tehran University of Medical Sciences Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Jaberi
authors.affiliation: Tehran University of Medical Sciences Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Tehran, Iran.
authors.forename: Naghmeh
authors.initials: N
authors.lastname: Rajaei
authors.affiliation: Tehran University of Medical Sciences Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Tehran, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Setayesh
keywords: Acute myeloid leukemia; Granulocyte colony-stimulating factor; RT-PCR; cytokines