title: An application of embryonic skin cells to repair diabetic skin wound: a wound reparation trail.
abstract: Cell therapy has shown its power to promote diabetic chronic wound healing. However, problems of scar formation and loss of appendages have not yet been solved. Our study aims to explore the potential of using embryonic skin cells (ESkCs) to repair diabetic wounds. Circular wound was created on the back of the diabetic mice, and ESkCs stained with CM-DIL were transplanted into the wound. Wound area was recorded at the day 4, 7, 11, and 14 after transplantation. The tissue samples were obtained at week 1, 2, and 3, and the tissue sections were stained by transforming growth factor β1 (TGF-β1), TGF-β3, vascular endothelial growth factor (VEGF), and CD31. The new skin formed on the wound of the diabetic mice with ESkC treatment at week 1 but not on the wounds of the non-treatment group. The histological scores of diabetic group with ESkC treatment were significantly better than the non-treatment group (P < 0.05). The fluorescence examination of CM-DIL and CD31 staining indicated that the ESkCs participated in the tissue regeneration, hair follicles formation, and angiogenesis. The expression of TGF-β1 and VEGF in ESkC-treated groups was noticeable in week 1 but disappeared in week 2. TGF-β3 was not expressed at week 1 but expressed markedly around hair follicles in week 2 in ESkC-treated groups. Our study demonstrated that ESkCs are capable of developing new skin with appendage restoration to repair the diabetic wounds.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shandong Province Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong 250014, China Department of Emergency Surgery, Shandong Province Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong 250014, China.
authors.forename: De Jian
authors.initials: de J
authors.lastname: Qian
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shandong Province Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong 250014, China School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan, Shandong 250014, China.
authors.forename: Xiang Kai
authors.initials: XK
authors.lastname: Guo
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai 200011, China.
authors.forename: Hui Chuan
authors.initials: HC
authors.lastname: Duan
authors.affiliation: Department of Cardiology, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
authors.forename: Zhi Hua
authors.initials: ZH
authors.lastname: Han
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shandong Province Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong 250014, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Meng
authors.affiliation: Laboratory of Microvascular Medicine, Medical Research Center, Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong, 250014, China wangyandr@126.com jliu0228@gmail.com.
authors.forename: Ju
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shandong Province Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong 250014, China wangyandr@126.com jliu0228@gmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D048909:Diabetes Complications; D004195:Disease Models, Animal; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008297:Male; D008810:Mice, Inbred C57BL; D008853:Microscopy; D012867:Skin / Q000033:anatomy & histology; D012879:Skin Physiological Phenomena; D014945:Wound Healing; D014947:Wounds and Injuries / Q000628:therapy*
keywords: Embryonic skin cells; TGF-β; diabetic wound; skin regeneration