title: A metabonomic study of adjuvant-induced arthritis in rats using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
abstract: Rheumatoid arthritis (RA) is a chronic systemic inflammatory and autoimmune disease accompanied by the destruction and deformities of joints. Adjuvant-induced arthritis (AIA) is one of the excellent animal models of RA used to understand disease pathogenesis and screen potential drugs. In this paper, a urinary metabonomics method based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) has been established to investigate the disease progression of AIA and find potential biomarkers of secondary inflammation in AIA rats. 24 potential biomarkers were identified, including xanthurenic acid, kynurenic acid, 4-pyridoxic acid, and phenylalanine, which revealed that tryptophan metabolism, phenylalanine metabolism, gut microbiota metabolism and energy metabolism were disturbed in AIA rats. These potential biomarkers and their corresponding pathways are helpful to further understand the mechanisms of AIA and pathogenesis of RA. This study demonstrates that metabonomics based on UPLC-Q-TOF-MS is a powerful methodology to analyze the underlying disease pathogenesis.
authors.affiliation: Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. mslab21@ciac.ac.cn liuzq@ciac.ac.cn.
authors.forename: Yao
authors.initials: Y
authors.lastname: Qi
authors.forename: Zifeng
authors.initials: Z
authors.lastname: Pi
authors.forename: Shu
authors.initials: S
authors.lastname: Liu
authors.forename: Fengrui
authors.initials: F
authors.lastname: Song
authors.forename: Na
authors.initials: N
authors.lastname: Lin
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism / Q000652:urine; D001835:Body Weight; D002853:Chromatography, Liquid / Q000379:methods; D007596:Joints / Q000378:metabolism / Q000473:pathology; D007737:Kynurenine / Q000378:metabolism; D008297:Male; D055442:Metabolome; D055432:Metabolomics; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014364:Tryptophan / Q000378:metabolism
chemical_list: D015415:Biomarkers; D007737:Kynurenine; D014364:Tryptophan