title: Changes in the number of circulating TCMand TEMsubsets in renal transplantation: relationship with acute rejection and induction therapy.
abstract: Effector (TEM) and central memory (TCM) T cells have been recently described as the main memory T-cell subsets generated after primary immune response, with a potential role in graft rejection after rechallenge with alloantigen. Because of their effector function, they could be involved in driving the response against the allograft, leading to rejection. In this study, we sought to investigate the different memory T-cell subpopulations in peripheral blood from a cohort of 90 patients who underwent consecutive renal transplant, and their association with acute rejection (AR) episodes and induction therapy. Twenty-one of them were monitored in the short term during the first 2 months after transplantation. Three of them suffered an AR but no changes in the circulating levels of either CD4+or CD8+TEMwere observed as compared with rejection-free renal transplant patients. In total, 69 patients out of 90 were monitored in the long term. Even 2 years after transplantation, maintained increased numbers of peripheral blood CD4+TEMwere observed in patients suffering with AR. Interestingly, induction therapy with thymoglobulin, but not with basiliximab, produced an increase in circulating CD4+TEMcells at 6 months after transplantation. In conclusion, our data suggest that AR episodes favor the induction of TEMcells in the periphery of renal transplant patients in the long term. It remains to be determined whether such an effect has any impact on long-term renal transplantation.
authors.affiliation: Servicios Inmunología, Hospital Universitario Marqués de Valdecilla-IFIMAV , Santander, Spain.
authors.forename: David S
authors.initials: DS
authors.lastname: Segundo
authors.affiliation: Nefrología, Hospital Universitario Marqués de Valdecilla-IFIMAV , Santander, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: Fernández-Fresnedo
authors.affiliation: Nefrología, Hospital Universitario Marqués de Valdecilla-IFIMAV , Santander, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Gago
authors.affiliation: Servicios Inmunología, Hospital Universitario Marqués de Valdecilla-IFIMAV , Santander, Spain.
authors.forename: Iñaki
authors.initials: I
authors.lastname: Beares
authors.affiliation: Servicios Inmunología, Hospital Universitario Marqués de Valdecilla-IFIMAV , Santander, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: González
authors.affiliation: Nefrología, Hospital Universitario Marqués de Valdecilla-IFIMAV , Santander, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Ruiz
authors.affiliation: Nefrología, Hospital Universitario Marqués de Valdecilla-IFIMAV , Santander, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Arias
authors.affiliation: Servicios Inmunología, Hospital Universitario Marqués de Valdecilla-IFIMAV , Santander, Spain.
authors.forename: Marcos
authors.initials: M
authors.lastname: López-Hoyos
keywords: acute rejection; effector T cells; kidney transplantation; memory T cells