title: Amelioration of LPS-induced inflammation response in microglia by AMPK activation.
abstract: Adenosine 5'-monophosphate-activated protein kinase (AMPK) is a key regulator of cellular energy homeostasis via modulating metabolism of glucose, lipid, and protein. In addition to energy modulation, AMPK has been demonstrated to associate with several important cellular events including inflammation. The results showed that ENERGI-F704 identified from bamboo shoot extract was nontoxic in concentrations up to 80 μM and dose-dependently induced phosphorylation of AMPK (Thr-172) in microglia BV2 cells. Our findings also showed that the treatment of BV2 with ENERGI-F704 ameliorated the LPS-induced elevation of IL-6 and TNF-α production. In addition, ENERGI-F704 reduced increased production of nitric oxide (NO) and prostaglandin E2 (PGE2) via downregulating the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2), respectively. Moreover, ENERGI-F704 decreased activated nuclear translocation and protein level of NF-κB. Inhibition of AMPK with compound C restored decreased NF-κB translocation by ENERGI-F704. In conclusion, ENERGI-F704 exerts inhibitory activity on LPS-induced inflammation through manipulating AMPK signaling and exhibits a potential therapeutic agent for neuroinflammatory disease.
authors.affiliation: Institute of Applied Science and Engineering, Catholic Fu-Jen University, New Taipei City 24205, Taiwan.
authors.forename: Chin-Chen
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Energenesis Biomedical Co., Ltd., New Taipei City 24205, Taiwan.
authors.forename: Jiun-Tsai
authors.initials: JT
authors.lastname: Lin
authors.affiliation: Energenesis Biomedical Co., Ltd., New Taipei City 24205, Taiwan.
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Cheng
authors.affiliation: Energenesis Biomedical Co., Ltd., New Taipei City 24205, Taiwan.
authors.forename: Cheng-Yi
authors.initials: CY
authors.lastname: Kuo
authors.affiliation: Energenesis Biomedical Co., Ltd., New Taipei City 24205, Taiwan.
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Huang
authors.affiliation: Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung City 40201, Taiwan.
authors.forename: Shao-Hsuan
authors.initials: SH
authors.lastname: Kao
authors.affiliation: Institute of Applied Science and Engineering, Catholic Fu-Jen University, New Taipei City 24205, Taiwan ; Department of Life Science, Catholic Fu-Jen University, New Taipei City 24205, Taiwan.
authors.forename: Yao-Jen
authors.initials: YJ
authors.lastname: Liang
authors.affiliation: Research Center for Industry of Human Ecology, Graduate Institute of Health Industry Technology, Department of Cosmetic Science, Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan.
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Cheng
authors.affiliation: Institute of Applied Science and Engineering, Catholic Fu-Jen University, New Taipei City 24205, Taiwan ; Energenesis Biomedical Co., Ltd., New Taipei City 24205, Taiwan ; Department of Life Science, Catholic Fu-Jen University, New Taipei City 24205, Taiwan.
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Chen
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D002460:Cell Line; D015232:Dinoprostone / Q000096:biosynthesis; D004734:Energy Metabolism / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D017628:Microglia / Q000166:cytology / Q000187:drug effects; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D031787:Sasa / Q000737:chemistry; D015533:Transcriptional Activation / Q000187:drug effects*
chemical_list: D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; D055372:AMP-Activated Protein Kinases; D015232:Dinoprostone