title: A multifunctional nanomicelle for real-time targeted imaging and precise near-infrared cancer therapy.
abstract: Simultaneous targeted cancer imaging, therapy and real-time therapeutic monitoring can prevent over- or undertreatment. This work describes the design of a multifunctional nanomicelle for recognition and precise near-infrared (NIR) cancer therapy. The nanomicelle encapsulates a new pH-activatable fluorescent probe and a robust NIR photosensitizer, R16FP, and is functionalized with a newly screened cancer-specific aptamer for targeting viable cancer cells. The fluorescent probe can light up the lysosomes for real-time imaging. Upon NIR irradiation, R16FP-mediated generation of reactive oxygen species causes lysosomal destruction and subsequently trigger lysosomal cell death. Meanwhile the fluorescent probe can reflect the cellular status and in situ visualize the treatment process. This protocol can provide molecular information for precise therapy and therapeutic monitoring.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China).
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Tian
authors.forename: Lin
authors.initials: L
authors.lastname: Ding
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Yang
authors.forename: Xilan
authors.initials: X
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Shen
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Jun-Sheng
authors.initials: JS
authors.lastname: Yu
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
mesh_terms: D000818:Animals; D052158:Aptamers, Peptide / Q000138:chemical synthesis / Q000627:therapeutic use*; D001896:Boron Compounds; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging; D005456:Fluorescent Dyes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007259:Infrared Rays / Q000627:therapeutic use*; D008247:Lysosomes / Q000473:pathology; D051379:Mice; D008823:Micelles; D008991:Monitoring, Physiologic; D053758:Nanoparticles; D009369:Neoplasms / Q000175:diagnosis* / Q000532:radiotherapy*; D017319:Photosensitizing Agents / Q000138:chemical synthesis* / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000737:chemistry
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D052158:Aptamers, Peptide; D001896:Boron Compounds; D005456:Fluorescent Dyes; D008823:Micelles; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species
keywords: cancer; fluorescence imaging; lysosome; photodynamic therapy; therapeutic monitoring