title: Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress.
abstract: Soil alkalinity is one of the most serious agricultural problems limiting crop yields. The legume Lotus tenuis is an important forage acknowledged by its ability to naturally grow in alkaline soils. To gain insight into the molecular responses that are activated by alkalinity in L. tenuis plants, subtractive cDNA libraries were generated from leaves and roots of these plants. Total RNAs of non-stressed plants (pH 5.8; E.C. 1.2), and plants stressed by the addition of 10 mM of NaHCO3 (pH 9.0; E.C. 1.9), were used as source of the driver and the tester samples, respectively. RNA samples were collected after 14 and 28 days of treatment. A total of 158 unigenes from leaves and 92 unigenes from roots were obtained and classified into 11 functional categories. Unigenes from these categories (4 for leaves and 8 for roots), that were related with nutrient metabolism and oxidative stress relief were selected, and their differential expression analyzed by qRT-PCR. These genes were found to be differentially expressed in a time dependent manner in L. tenuis during the alkaline stress application. Data generated from this study will contribute to the understanding of the general molecular mechanisms associated to plant tolerance under long-term alkaline stress in plants.
authors.affiliation: Grupo INTERBIODES (Interacciones Biológicas del Desierto/Biological Interactions of Desert), CIGEOBIO (FCEFyN, UNSJ/CONICET), Dpto. de Biología, Av. Ignacio de la Roza 590 (Oeste), J5402DCS Rivadavia, San Juan, Argentina. Electronic address: rpaz@fca.uncu.edu.ar.
authors.forename: Rosalía Cristina
authors.initials: RC
authors.lastname: Paz
authors.affiliation: Unidad de Biotecnología 1, IIB-IINTECH/UNSAM-CONICET, Chascomús, Buenos Aires, Argentina. Electronic address: rubenrocco@intech.gov.ar.
authors.forename: Rubén Anibal
authors.initials: RA
authors.lastname: Rocco
authors.affiliation: Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), Camino a la Presa de San José No. 2055, Lomas 4a Sección, CP 78216 San Luis Potosí, SLP, México. Electronic address: jbremont@ipicyt.edu.mx.
authors.forename: Juan Francisco
authors.initials: JF
authors.lastname: Jiménez-Bremont
authors.affiliation: Facultad de Ciencias, Universidad Autónoma de San Luis Potosí, Av. Salvador Nava s/n, Zona Universitaria, C.P. 78290 San Luis Potosí, SLP, México. Electronic address: mrodriguez@fc.uaslp.mx.
authors.forename: Margarita
authors.initials: M
authors.lastname: Rodríguez-Kessler
authors.affiliation: Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), Camino a la Presa de San José No. 2055, Lomas 4a Sección, CP 78216 San Luis Potosí, SLP, México. Electronic address: abecerra@ipicyt.edu.mx.
authors.forename: Alicia
authors.initials: A
authors.lastname: Becerra-Flora
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina; PROPLAME-PRHIDEB (CONICET), Argentina. Electronic address: anamen@bg.fcen.uba.ar.
authors.forename: Ana Bernardina
authors.initials: AB
authors.lastname: Menéndez
authors.affiliation: Unidad de Biotecnología 1, IIB-IINTECH/UNSAM-CONICET, Chascomús, Buenos Aires, Argentina. Electronic address: ruiz@intech.gov.ar.
authors.forename: Oscar Adolfo
authors.initials: OA
authors.lastname: Ruíz
mesh_terms: D000863:Antacids / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D000070116:Lotus / Q000187:drug effects / Q000235:genetics*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics*; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics*; D066251:Subtractive Hybridization Techniques
chemical_list: D000863:Antacids
keywords: Adaptive response; Alkaline stress; Long-term response; Lotus tenuis; Root and shoot tissues; Stress induced transcripts; Suppressive Subtractive Hybridization