title: Lactate production as representative of the fermentation potential of Corynebacterium glutamicum 2262 in a one-step process.
abstract: The fermentative properties of thermo-sensitive strain Corynebacterium glutamicum 2262 were investigated in processes coupling aerobic cell growth and the anaerobic fermentation phase. In particular, the influence of two modes of fermentation on the production of lactate, the fermentation product model, was studied. In both processes, lactate was produced in significant amount, 27 g/L in batch culture, and up to 55.8 g/L in fed-batch culture, but the specific production rate in the fed-batch culture was four times lower than that in the batch culture. Compared to other investigated fermentation processes, our strategy resulted in the highest yield of lactic acid from biomass. Lactate production by C. glutamicum 2262 thus revealed the capability of the strain to produce various fermentation products from pyruvate.
authors.affiliation: a Laboratoire Réactions et Génie des Procédés , UMR CNRS 7274, ENSAIA - Université de Lorraine , Vandoeuvre Cedex , France.
authors.forename: Hoang Bao Truc
authors.initials: HB
authors.lastname: Khuat
authors.forename: Abdoul Karim
authors.initials: AK
authors.lastname: Kaboré
authors.forename: Eric
authors.initials: E
authors.lastname: Olmos
authors.forename: Michel
authors.initials: M
authors.lastname: Fick
authors.forename: Joseph
authors.initials: J
authors.lastname: Boudrant
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Goergen
authors.forename: Stéphane
authors.initials: S
authors.lastname: Delaunay
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guedon
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D061249:Batch Cell Culture Techniques; D049109:Cell Proliferation; D048230:Corynebacterium glutamicum / Q000166:cytology / Q000378:metabolism*; D005285:Fermentation; D019344:Lactic Acid / Q000096:biosynthesis*; D010100:Oxygen / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D019344:Lactic Acid; D010100:Oxygen
keywords: Corynebacterium glutamicum; batch; fed batch; fermentation; lactate