title: Structural determination of an N-glycan moiety attached to the prothoracicotropic hormone from the silkmoth Bombyx mori.
abstract: The predominant structure of the N-glycan on the prothoracicotropic hormone (PTTH) isolated from 1.8 million adult heads of silkmoths was determined to be Manα1-6Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAc-OH, which is identical to that of the baculovirus-expressed recombinant PTTH. An ecdysis progression assay demonstrated that N-glycosylated PTTH exhibited a slightly higher activity than the recombinant PTTH without N-glycosylation produced by an Escherichia coli expression system.
authors.affiliation: a Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo , Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Nagata
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kataoka
authors.forename: Akinori
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D002240:Carbohydrate Sequence; D007301:Insect Hormones / Q000737:chemistry*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D007301:Insect Hormones; D011134:Polysaccharides; C009089:prothoracicotropic hormone
keywords: Bombyx mori; N-glycan, peptide; metamorphosis; prothoracicotropic hormone