title: Transcriptional regulation of genes encoding ABA metabolism enzymes during the fruit development and dehydration stress of pear 'Gold Nijisseiki'.
abstract: To investigate the contribution of abscisic acid (ABA) in pear 'Gold Nijisseiki' during fruit ripening and under dehydration stress, two cDNAs (PpNCED1 and PpNCED2) which encode 9-cis-epoxycarotenoid dioxygenase (NCED) (a key enzyme in ABA biosynthesis), two cDNAs (PpCYP707A1 and PpCYP707A2) which encode 8'-hydroxylase (a key enzyme in the oxidative catabolism of ABA), one cDNA (PpACS3) which encodes 1-aminocyclopropane-1-carboxylic acid (ACC), and one cDNA (PpACO1) which encodes ACC oxidase involved in ethylene biosynthesis were cloned from 'Gold Nijisseiki' fruit. In the pulp, peel and seed, expressions of PpNCED1 and PpNCED2 rose in two stages which corresponded with the increase of ABA levels. The expression of PpCYP707A1 dramatically declined after 60-90 days after full bloom (DAFB) in contrast to the changes of ABA levels during this period, while PpCYP707A2 stayed low during the whole development of fruit. Application of exogenous ABA at 100 DAFB increased the soluble sugar content and the ethylene release but significantly decreased the titratable acid and chlorophyll contents in fruits. When fruits harvested at 100 DAFB were stored in the laboratory (25 °C, 50% relative humidity), the ABA content and the expressions of PpNCED1/2 and PpCYP707A1 in the pulp, peel and seed increased significantly, while ethylene reached its highest value after the maximum peak of ABA accompanied with the expressions of PpACS3 and PpACO1. In sum the endogenous ABA may play an important role in the fruit ripening and dehydration of pear 'Gold Nijisseiki' and the ABA level was regulated mainly by the dynamics of PpNCED1, PpNCED2 and PpCYP707A1 at the transcriptional level.
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: daishengjie1988@163.com.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Dai
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: hbliping2006@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: 529210500@qq.com.
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: woshiliqian0204@126.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: peiyuelincau@126.com.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Pei
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: 790182596@qq.com.
authors.forename: Suihuan
authors.initials: S
authors.lastname: He
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: 269299478@qq.com.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: 763913588@qq.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: kwb19910520@163.com.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Kai
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: 1074854034@qq.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: yalan@cau.edu.cn.
authors.forename: Yalan
authors.initials: Y
authors.lastname: Liao
authors.affiliation: College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, PR China. Electronic address: pleng@cau.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Leng
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D005638:Fruit / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D031989:Pyrus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid
keywords: ABA; Dehydration; Ethylene; Pear fruit; PpCYP707As; PpNCEDs