title: Ozone therapy ameliorates paraquat-induced lung injury in rats.
abstract: Paraquat (PQ) overdose can cause acute lung injury and death. Ozone therapy (OT) was previously demonstrated to alleviate inflammation and necrosis in various pathologies. We therefore hypothesized that OT has ameliorative and preventive effects on PQ-induced lung damage due to anti-inflammatory and antioxidants properties. Sprague-Dawley rats (n = 24) were separated into three groups: sham, PQ, and PQ+OT groups. 15 mg/kg PQ was administered intraperitoneally in PQ and PQ+OT groups to induce experimental lung injury. One hour after PQ treatment, PQ+OT group was administered a single dose of ozone-oxygen mixture (1 mg/kg/day) by intraperitoneal route for four consecutive days. The animals were sacrificed on fifth day after PQ administration. Blood samples and lung tissues were collected to evaluate the inflammatory processes, antioxidant defense and pulmonary damage. Serum lactate dehydrogenase (LDH) and neopterin levels, tissue oxidative stress parameters, total TGF-β1 levels, and histological injury scores in PQ+OT group were significantly lower than PQ group (P<0.05, PQ vs. PQ+OT). Total antioxidant capacity in PQ+OT group was significantly higher than PQ group (P < 0.05, PQ+OT vs. PQ). These findings suggest that outcome in PQ-induced lung injury may be improved by using OT as an adjuvant therapy.
authors.affiliation: Department of Emergency Medicine Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey.
authors.forename: Umit
authors.initials: U
authors.lastname: Kaldirim
authors.affiliation: Department of Physiology, Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey buysal@gata.edu.tr.
authors.forename: Bulent
authors.initials: B
authors.lastname: Uysal
authors.affiliation: Department of Physiology, Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Yuksel
authors.affiliation: Department of Toxicolgy, Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey.
authors.forename: Enis
authors.initials: E
authors.lastname: Macit
authors.affiliation: Department of Emergency Medicine Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey.
authors.forename: Yusuf E
authors.initials: YE
authors.lastname: Eyi
authors.affiliation: Department of Forensic Medicine, Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Toygar
authors.affiliation: Department of Emergency Medicine Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey.
authors.forename: Salim K
authors.initials: SK
authors.lastname: Tuncer
authors.affiliation: Department of Emergency Medicine Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey.
authors.forename: Sukru
authors.initials: S
authors.lastname: Ardic
authors.affiliation: Department of Emergency Medicine Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Arziman
authors.affiliation: Department of Biochemistry, Sarikamis Military Hospital, Sarikamis, 36500 Kars, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Aydin
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Hacettepe University, Sihhiye 06100, Ankara, Turkey.
authors.forename: Yesim
authors.initials: Y
authors.lastname: Oztas
authors.affiliation: Department of Pathology, Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey.
authors.forename: Yildirim
authors.initials: Y
authors.lastname: Karslioglu
authors.affiliation: Department of Physiology, Gulhane Military Medical Academy, 06010 Etlik, Ankara, Turkey.
authors.forename: Turgut
authors.initials: T
authors.lastname: Topal
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D001774:Blood Chemical Analysis; D005260:Female; D007274:Injections, Intraperitoneal; D008168:Lung / Q000473:pathology; D055370:Lung Injury / Q000139:chemically induced* / Q000628:therapy*; D016877:Oxidants / Q000633:toxicity*; D010126:Ozone / Q000627:therapeutic use*; D010269:Paraquat / Q000633:toxicity*; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016877:Oxidants; D010126:Ozone; D010269:Paraquat
keywords: Paraquat; TGF-β1; lung injury; neopterin; oxidative stress; ozone therapy