title: Selective isolation of β-glucan from corn pericarp hemicelluloses by affinity chromatography on cellulose column.
abstract: A combination of anion-exchange chromatography and affinity chromatography on a cellulose column was found to be effective for the isolation of β-(1,3;1,4)-glucan (BG) from corn pericarp hemicelluloses (CPHs). CPHs containing 6.6% BG were extracted from corn pericarp with 6M urea-2 wt% NaOH solution and initially fractionated into neutral and acidic parts by anion exchange chromatography to remove acidic arabinoxylan consisting of arabinose (35.6%) and xylose (50.9%). The neutral fraction (yield; 10.1% on the basis of CPHs) consisting of 1.0% arabinose, 10.1% xylose and 80.3% glucose containing 28.4% BG was then applied to a cellulose column of Whatman CF-11. BG could be recovered from the adsorbed fraction on the cellulose column by elution with 2% NaOH in a yield of 2.6% on the basis of CPHs with a purity of 84.7%. The chemical structure of the isolated corn pericarp BG was confirmed by (13)C NMR spectroscopic, methylation and lichenase treatment analyses. The results indicate that the ratios of (1,4)/(1,3) linkage and cellotriosyl/cellotetraosyl segments of the BG were 2.60 and 2.5, respectively.
authors.affiliation: Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: tonki@kais.kyoto-u.ac.jp.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yoshida
authors.affiliation: Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Honda
authors.affiliation: Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Yamadaoka, Suita 565-0871, Osaka, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsujimoto
authors.affiliation: Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Yamadaoka, Suita 565-0871, Osaka, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uyama
authors.affiliation: Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Yamadaoka, Suita 565-0871, Osaka, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Azuma
mesh_terms: D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D011134:Polysaccharides / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*; D047071:beta-Glucans / Q000302:isolation & purification*
chemical_list: D011134:Polysaccharides; D047071:beta-Glucans; C007916:hemicellulose
keywords: Affinity chromatography; Cellulose column; Corn pericarp hemicelluloses; β-Glucan