title: Serology of paracoccidioidomycosis due to Paracoccidioides lutzii.
abstract: Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. In western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.
authors.affiliation: Universidade Federal de São Paulo (UNIFESP), Departamento de Microbiologia, Imunologia e Parasitologia, Disciplina de Biologia Celular, São Paulo, São Paulo, Brasil.
authors.forename: Gregory
authors.initials: G
authors.lastname: Gegembauer
authors.affiliation: Universidade Federal de São Paulo (UNIFESP), Departamento de Microbiologia, Imunologia e Parasitologia, Disciplina de Biologia Celular, São Paulo, São Paulo, Brasil.
authors.forename: Leticia Mendes
authors.initials: LM
authors.lastname: Araujo
authors.affiliation: Faculdade de Medicina (FAMED) da Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brasil.
authors.forename: Edy Firmina
authors.initials: EF
authors.lastname: Pereira
authors.affiliation: Universidade Federal de São Paulo (UNIFESP), Departamento de Microbiologia, Imunologia e Parasitologia, Disciplina de Biologia Celular, São Paulo, São Paulo, Brasil.
authors.forename: Anderson Messias
authors.initials: AM
authors.lastname: Rodrigues
authors.affiliation: Faculdade de Medicina (FAMED) da Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brasil.
authors.forename: Anamaria Mello Miranda
authors.initials: AM
authors.lastname: Paniago
authors.affiliation: Núcleo de Doenças Infecciosas e Tropicais, Universidade Federal do Mato Grosso (UFMT), Cuiabá, Mato Grosso, Brasil.
authors.forename: Rosane Christine
authors.initials: RC
authors.lastname: Hahn
authors.affiliation: Universidade Federal de São Paulo (UNIFESP), Departamento de Microbiologia, Imunologia e Parasitologia, Disciplina de Biologia Celular, São Paulo, São Paulo, Brasil.
authors.forename: Zoilo Pires
authors.initials: ZP
authors.lastname: de Camargo
mesh_terms: D000328:Adult; D000368:Aged; D000946:Antigens, Fungal / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western; D001938:Brazil; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D008297:Male; D008875:Middle Aged; D010228:Paracoccidioides / Q000276:immunology / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology; D055815:Young Adult
chemical_list: D000946:Antigens, Fungal