title: Cellulolytic and xylanolytic enzymes from thermophilic Aspergillus terreus RWY.
abstract: Thermophilic Aspergillus terreus RWY produced cellulases and xylanases in optimal concentrations at 45 °C in solid state fermentation process, though enzyme production was also observed at 50 and 55 °C. Filter paper cellulase (FP), endoglucanase (EG), β-glucosidase (BGL), cellobiohydrolase (CBH), xylanase, β-xylosidase, α-L-arabinofuranosidase and xylan esterase activities for A. terreus RWY at 45 °C in 72 h were 11.3 ± 0.65, 103 ± 6.4, 122.5 ± 8.7, 10.3 ± 0.66, 872 ± 22.5, 22.1 ± 0.75, 126.4 ± 8.4 and 907 ± 15.5 U (g-ds)(-1) , respectively. Enzyme was optimally active at temperatures and pH ranging between 50-60 °C and 4.0-6.0, respectively. The half life (T1/2 ) of 270 and 240 min at 70 and 75 °C, respectively for the enzyme indicates its stability at higher temperatures. The addition of MnCl2 , CoCl2 , and FeCl3 significantly enhanced cellulase activity. Enzyme demonstrated multiplicity by having seven, one and three isoform(s) for EG, CBH and BGL, respectively. Significant production of functionally active consortium of cellulolytic and xylanolytic enzymes from A. terreus RWY makes it a potential candidate in bioprocessing applications.
authors.affiliation: Central Institute of Post-Harvest Engineering and Technology, P.O. PAU, Ludhiana, Punjab, India; Department of Microbiology, Punjab Agricultural University, Ludhiana, Punjab, India.
authors.forename: Reetika
authors.initials: R
authors.lastname: Sharma
authors.forename: Gurvinder Singh
authors.initials: GS
authors.lastname: Kocher
authors.forename: Ravinder Singh
authors.initials: RS
authors.lastname: Bhogal
authors.forename: Harinder Singh
authors.initials: HS
authors.lastname: Oberoi
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D044602:Cellulases / Q000096:biosynthesis* / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis* / Q000737:chemistry; D004795:Enzyme Stability; D005296:Ferrous Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D017895:Manganese Compounds / Q000737:chemistry
chemical_list: D002712:Chlorides; D005296:Ferrous Compounds; D017895:Manganese Compounds; D003035:Cobalt; D044602:Cellulases; D043364:Endo-1,4-beta Xylanases; C018021:cobaltous chloride; C025340:manganese chloride; C029451:ferrous chloride
keywords: Aspergillus terreus RWY; Bioprocessing; Cellulolytic; Thermophilic enzymes; Xylanolytic