title: Comparison of the genomes and transcriptomes associated with the different protease secretions of Aspergillus oryzae 100-8 and 3.042.
abstract: Aspergillus oryzae is used to produce traditional fermented foods and beverages. A. oryzae 3.042 produces a neutral protease and an alkaline protease but rarely an acid protease, which is unfavourable to soy-sauce fermentation. A. oryzae 100-8 was obtained by N(+) ion implantation mutagenesis of A. oryzae 3.042, and the protease secretions of these two strains are different. Sequencing the genome of A. oryzae 100-8 and comparing it to the genomes of A. oryzae 100-8 and 3.042 revealed some differences, such as single nucleotide polymorphisms, nucleotide deletion or insertion. Some of these differences may reflect the ability of A. oryzae to secrete proteases. Transcriptional sequencing and analysis of the two strains during the same growth processes provided further insights into the genes and pathways involved in protease secretion.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Ministry of Education, Tianjin, 300457, People's Republic of China, zgzjiangnan@163.com.
authors.forename: Guozhong
authors.initials: G
authors.lastname: Zhao
authors.forename: Yunping
authors.initials: Y
authors.lastname: Yao
authors.forename: Lihua
authors.initials: L
authors.lastname: Hou
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Cao
mesh_terms: D001236:Aspergillus oryzae / Q000145:classification* / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D014644:Genetic Variation; D016681:Genome, Fungal; D007218:Industrial Microbiology; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D005656:Fungal Proteins; D010447:Peptide Hydrolases