title: Presence of osteoinductive factors in bovine colostrum.
abstract: New approaches in the treatment of skeletal defects may benefit from the use of soluble biological factors. We previously standardized a derivative of bovine colostrum (SBCD), deprived of casein and fat and rich in cytokines. In the present study, we tested its possible use as an adjuvant in bone healing. SBCD contained factors involved in stromal cell stimulation and differentiation and induced cytokine production from stimulated mesenchymal stem cells (MSCs). In vitro, SBCD promoted proliferation, migration and, in association with osteogenic factors, osteogenic differentiation of osteoblastic and MSCs. In in vivo experiments of subcutaneous Matrigel injection in mice, SBCD plus hydroxyapatite, but not hydroxyapatite nor SBCD alone, induced recruitment of macrophages and stromal cells. After 60 days, plugs containing SBCD and hydroxyapatite were densely calcified and diffusely positive for osteocalcin, supporting the occurrence of an early osteogenic process. These results indicate that SBCD is a rich source of factors with osteoinductive properties.
authors.affiliation: a Department of Surgical Sciences, Dental School , University of Turin , Torino , Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Mussano
authors.forename: Alberto
authors.initials: A
authors.lastname: Bartorelli Cusani
authors.forename: Alessia
authors.initials: A
authors.lastname: Brossa
authors.forename: Stefano
authors.initials: S
authors.lastname: Carossa
authors.forename: Gianni
authors.initials: G
authors.lastname: Bussolati
authors.forename: Benedetta
authors.initials: B
authors.lastname: Bussolati
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003126:Colostrum / Q000737:chemistry*; D017886:Durapatite / Q000378:metabolism; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D011247:Pregnancy
chemical_list: D017886:Durapatite
keywords: colostrum; hydroxyapatite; mesenchymal stem cells; osteogenesis