title: Signal amplification of glucosamine-6-phosphate based on ribozyme glmS.
abstract: Ribozyme glmS based isothermal amplification assay is developed for the colorimetric detection of glucosamine-6-phosphate (GlcN6P). Upon binding to the metabolite target GlcN6P, self-cleavage of glmS ribozyme is initiated to release RNA fragment that can trigger the cascade signal amplification to release large amount of G-quadruplex DNAzymes as reporter for colorimetric detection. Given the importance of GlcN6P for cell wall biosynthesis, the glmS riboswitch has become a new drug target for the development of antibiotics. This assay not only offers a convenient detection of GlcN6P with high specificity and sensitivity, but also provides a platform for high-throughput screening of antibiotics based on glmS riboswitches.
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, PR China.
authors.forename: Yongyun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, PR China.
authors.forename: Haodong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Du
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, PR China.
authors.forename: Afshan
authors.initials: A
authors.lastname: Yasmeen
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Dong
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, PR China. Electronic address: cuixin@cib.ac.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Cui
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, PR China. Electronic address: tangzhuo@cib.ac.cn.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Tang
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000032:analysis; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives* / Q000032:analysis; D021141:Nucleic Acid Amplification Techniques; D016337:RNA, Catalytic / Q000235:genetics; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
chemical_list: D001426:Bacterial Proteins; D005944:Glucosamine; D019298:Glucose-6-Phosphate; D016337:RNA, Catalytic; C001293:glucosamine 6-phosphate; C000731191:component S, glutamate mutase protein, Bacteria; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
keywords: Colorimetric assay; Glucosamine-6-phosphate; Isothermal amplification; Strand-displacement amplifications; glmS riboswitch