title: Undibacterium squillarum sp. nov., isolated from a freshwater shrimp culture pond.
abstract: A bacterial strain designated CMJ-15(T) was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-15(T) were facultatively anaerobic, Gram-stain-negative and motile by a single polar flagellum and formed light-yellow colonies. Growth occurred at 20-40 °C (optimum, 25-30 °C), with 0-0.7% NaCl (optimum, 0-0.2%) and at pH 5.0-9.0 (optimum, pH 6.0-7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-15(T) belonged to the genus Undibacterium and its closest neighbour was Undibacterium oligocarboniphilum EM 1(T), with sequence similarity of 96.3%. The predominant cellular fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16:0 and C18:1ω7c. The major cellular hydroxy fatty acid was C10:0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 52.2 mol%. On the basis of these phylogenetic and phenotypic data, strain CMJ-15(T) should be classified as representing a novel species, for which the name Undibacterium squillarum sp. nov. is proposed. The type strain is CMJ-15(T) ( = BCRC 80404(T) = LMG 26892(T) = KCTC 23917(T)).
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Yang-Shun
authors.initials: YS
authors.lastname: Lin
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Jhen-Ci
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd Nan-Tzu, Kaohsiung City 811, Taiwan.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
mesh_terms: D000818:Animals; D017756:Aquaculture; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D033364:Decapoda; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D042802:Oxalobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D010858:Pigmentation; D061005:Ponds / Q000382:microbiology*; D011700:Putrescine / Q000031:analogs & derivatives / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; C002952:2-hydroxyputrescine; D011700:Putrescine