title: Interactions between acidified dispersions of milk proteins and dextran or dextran sulfate.
abstract: Polysaccharides are often used to stabilize cultured milk products, although the nature of these interactions is not entirely clear. The objective of this study was to investigate phase behavior of milk protein dispersions with added dextran (DX; molecular weight = 2 × 10(6) Da) or dextran sulfate (DS; molecular weight = 1.4 × 10(6) Da) as examples of uncharged and charged polysaccharides, respectively. Reconstituted skim milk (5-20% milk solids, wt/wt) was acidified to pH 4.4, 4.6, 4.8, or 4.9 at approximately 0°C (to inhibit gelation) by addition of 3 N HCl. Dextran or DS was added to acidified milk samples to give concentrations of 0 to 2% (wt/wt) and 0 to 1% (wt/wt) polysaccharide, respectively. Milk samples were observed for possible phase separation after storage at 0°C for 1 and 24h. Possible gelation of these systems was determined by using dynamic oscillatory rheology. The type of interactions between caseins and DX or DS was probed by determining the total carbohydrate analysis of supernatants from phase-separated samples. At 5.0 to 7.5% milk solids, phase separation of milk samples occurred after 24h even without DX or DS addition, due to destabilization of caseins in these acidic conditions, and a stabilizing effect was observed when 0.7 or 1.0% DS was added. At higher milk solids content, phase separation was not observed without DX or DS addition. Similar results were observed at all pH levels. Gelation occurred in samples containing high milk solids (≥10%) with the addition of 1.0 to 2.0% DX or 0.4 to 1.0% DS. Based on carbohydrate analysis of supernatants, we believe that DX interacted with milk proteins through a type of depletion flocculation mechanism, whereas DS appeared to interact via electrostatic-type interactions with milk proteins. This study helps to explain how uncharged and charged stabilizers influence the texture of cultured dairy products.
authors.affiliation: Department of Food Science, University of Wisconsin-Madison, 1605 Linden Drive, Madison 53706.
authors.forename: U
authors.initials: U
authors.lastname: Pachekrepapol
authors.affiliation: Wisconsin Center for Dairy Research, University of Wisconsin-Madison, 1605 Linden Drive, Madison 53706.
authors.forename: D S
authors.initials: DS
authors.lastname: Horne
authors.affiliation: Wisconsin Center for Dairy Research, University of Wisconsin-Madison, 1605 Linden Drive, Madison 53706. Electronic address: jlucey@cdr.wisc.edu.
authors.forename: J A
authors.initials: JA
authors.lastname: Lucey
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry; D016264:Dextran Sulfate / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000737:chemistry*; D008970:Molecular Weight; D012212:Rheology; D014783:Viscosity
chemical_list: D002364:Caseins; D003911:Dextrans; D005782:Gels; D008894:Milk Proteins; D016264:Dextran Sulfate
keywords: cultured dairy product; depletion flocculation; dextran