title: Resveratrol increases nephrin and podocin expression and alleviates renal damage in rats fed a high-fat diet.
abstract: Resveratrol is well known for its anti-inflammation and anti-oxidant properties, and has been shown to be effective in alleviating the development of obesity. The purpose of this investigation was to analyze the effect of resveratrol on renal damage in obese rats induced by a high-fat diet (HFD) and its possible mechanisms. Male Sprague-Dawley rats were divided into three groups: control, HFD, and HFD plus resveratrol (treated with 100 mg/kg/day resveratrol). Body weight, serum and urine metabolic parameters, and kidney histology were measured. Meanwhile, the activities of nuclear factor-κB (NF-κB) and superoxide dismutase (SOD), the content of malondialdehyde (MDA), and the protein levels of tumor necrosis factor (TNF-α), monocyte chemotactic protein-1 (MCP-1), nephrin and podocin in kidney were detected. Our work showed that resveratrol alleviated dyslipidemia and renal damage induced by HFD, decreased MDA level and increased SOD activity. Furthermore, the elevated NF-κB activity, increased TNF-α and MCP-1 levels, and reduced expressions of nephrin and podocin induced by HFD were significantly reversed by resveratrol. These results suggest resveratrol could ameliorate renal injury in rats fed a HFD, and the mechanisms are associated with suppressing oxidative stress and NF-κB signaling pathway that in turn up-regulate nephrin and podocin protein expression.
authors.affiliation: Department of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China. panqingrongcn@163.com.
authors.forename: Qing-Rong
authors.initials: QR
authors.lastname: Pan
authors.affiliation: Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. renyanlong@yeah.net.
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Ren
authors.affiliation: Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. zhujiajia83@126.com.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Zhu
authors.affiliation: Department of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China. longwesthill@163.com.
authors.forename: Yan-Jin
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: Department of Traditional Chinese Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China. rosamand@163.com.
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Zheng
authors.affiliation: Department of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China. newmoon163@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China. xuyuan32001@sina.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China. drwg6688@163.com.
authors.forename: Guang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. liuwenxian20@sina.com.
authors.forename: Wen-Xian
authors.initials: WX
authors.lastname: Liu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D005951:Glucose Tolerance Test; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001786:Blood Glucose; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D016328:NF-kappa B; C407223:NPHS2 protein; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; C113267:nephrin; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D000077185:Resveratrol