title: RNA-binding protein research with transcriptome-wide technologies in neural development.
abstract: An increasing number of studies have demonstrated that neural RNA-binding proteins (nRNABPs) participate in several steps of neural development through post-transcriptional regulation of their RNA targets (Grabowski Curr Opin Genet Dev 21:388-394, 2011). Classical genetics and in vitro biochemical approaches have identified several important RNA targets of nRNABPs linked to cell-fate decision and neuronal functions. In recent years, new technologies, such as unbiased in vivo protein-RNA interaction approaches, high-throughput sequencing-cross-linked immunoprecipitation (HITS-CLIP), microarrays, RNAseq and others, have been developed. The use of these with genetics has succeeded in defining a dynamic range of RNA targets of RNABPs at the transcriptome-wide level. This new platform also provides the mechanistic insights into a specific biological function of nRNABPs. This review highlights the discoveries and challenges of the interplay between the nRNABPs and their biological functions in neural development.
authors.affiliation: Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan, myano@a2.keio.jp.
authors.forename: Masato
authors.initials: M
authors.lastname: Yano
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ohtsuka
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D009420:Nervous System / Q000254:growth & development*; D055495:Neurogenesis; D009474:Neurons / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012106:Research
chemical_list: D016601:RNA-Binding Proteins