title: Effect of gibberellic acid (GA), indole acetic acid (IAA) and benzylaminopurine (BAP) on the synthesis of essential oils and the isomerization of methyl chavicol and trans-anethole in Ocimum gratissimum L.
abstract: Basil (O. gratissimum L) is a aromatic and medicinal plant widely used in traditional medicine in Morocco. The aim of this work was to study the effect of three plant growth regulators gibberellic acid (GA), indole 3-acetic acid (IAA) and benzylaminopurine (BAP) on the content and composition of essential oils of this plant, especially on the main compound (methyl chavicol) and its isomer (the trans-anethole). The results showed a wide variation on yield, content and range of the molecule constituent of oil, with a balance of appearances and/or disappearances of a few molecules. GA caused a slight decrease in the oil yield (0.2%), but it increased the diversity of compounds (17 molecules) with the appearance of four new compounds (naphthalene, camphor, germacrene-D, and ledene) and disappearance of (β cedrene, azulene). This variation also caused a very important decrease in the main compound (methyl chavicol) and increases its isomer (trans-anethole). IAA and BAP caused an increase in the yield of essential oil (0.30% and 0.32% respectively) without much influence on the main compounds, but with some change in the composition such as the appearance of (germacrene-D) and the disappearance of (aristolene).
authors.affiliation: Laboratory of Bioactive Molecules: Structure and Function, Faculty of Science and Technology Fez, B.P. 2202, Route d'Imouzzer, FEZ Morocco.
authors.forename: Zakaria
authors.initials: Z
authors.lastname: Hazzoumi
authors.affiliation: Laboratory of Bioactive Molecules: Structure and Function, Faculty of Science and Technology Fez, B.P. 2202, Route d'Imouzzer, FEZ Morocco.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Moustakime
authors.affiliation: Laboratory of Bioactive Molecules: Structure and Function, Faculty of Science and Technology Fez, B.P. 2202, Route d'Imouzzer, FEZ Morocco.
authors.forename: Khalid
authors.initials: K
authors.lastname: Amrani Joutei
keywords: Essential oil; Methyl chavicol; O. gratissimum L; Plant growth regulators; Trans-anethole