title: In vitro antioxidant activity of Retama monosperma (L.) Boiss.
abstract: The relationship between the antioxidant activity and the phenolic contents (total polyphenol, flavonoid and condensed tannin) of Retama monosperma (Fabaceae), used commonly in the traditional medicine of Mediterranean regions, was investigated. The antioxidant activities of the various fractions (toluene, chloroform, ethyl acetate and butanol) of the hydromethanolic extract of the seeds, stems and flowers have been evaluated using in vitro 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) radical scavenging activities and Phosphomolybdic acid assays and were compared to ascorbic acid. A significant high Pearson's correlations between flavonoid content and antioxidant activities (r = 0.91) with Phosphomolybdic acid assays and (r = - 0.79) with IC50 DPPH radical scavenging activities. However, there was no correlation between condensed tannin and antioxidant activities. The results obtained in the present study indicate that the ethyl acetate fraction of seeds is a potential source of natural antioxidant for R. monosperma.
authors.affiliation: a Laboratoire des Productions Valorisations Végétales et Microbiennes (LP2VM) , Faculty of Natural Sciences and Life, University of Sciences and Technology, Mohamed Boudiaf (USTOMB) , Oran 31000 , Algeria.
authors.forename: Zoubir
authors.initials: Z
authors.lastname: Belmokhtar
authors.forename: Meriem Kaid
authors.initials: MK
authors.lastname: Harche
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D066298:In Vitro Techniques; D008519:Medicine, Traditional; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D010851:Picrates / Q000494:pharmacology; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D013634:Tannins / Q000032:analysis / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D059808:Polyphenols; D013634:Tannins; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Retama monosperma; antioxidant; polyphenol; relationship