title: Over-inhibition of NADPH oxidase reduce the wound healing in liver of finfish.
abstract: Wound healing is a complex process involving soluble mediators, blood cells, extracellular matrix, and parenchymal cells in a response that occurs after surgical procedure or traumatic injury. The present study aims to investigate the ROS producing from the injury that involved in the wound healing using the ZFL (zebrafish liver cell) and tilapia partial hepatectomy model. In the ZFL, we observed that while over-inhibition of NADPH activity leading to reduce the wound healing moreover, experiment of the oxidative stress by the extracellular hydrogen peroxide exactly presented to increase the PCNA, BrdU and Ki-67 histopathological repair response of tilapia liver follow partial hepatectomy. We conclude that over inhibition of the NADPH oxidase by DPI may reduce the cell even the tissue in the progress of healing after the injury.
authors.affiliation: Institute of Fisheries Science, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Wu
authors.affiliation: Freshwater Aquaculture Research Center, Fisheries Research Institute, Council of Agriculture, Changhua 50562, Taiwan.
authors.forename: Shih-Ling
authors.initials: SL
authors.lastname: Huang
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung 20248, Taiwan.
authors.forename: Fan-Hua
authors.initials: FH
authors.lastname: Nan
authors.affiliation: Institute of Fisheries Science, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Ching-Sheng
authors.initials: CS
authors.lastname: Chang
authors.affiliation: Institute of Fisheries Science, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Chien-Mei
authors.initials: CM
authors.lastname: Hsiao
authors.affiliation: College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Kam-Chiu
authors.initials: KC
authors.lastname: Lai
authors.affiliation: Institute of Fisheries Science, National Taiwan University, Taipei 10617, Taiwan; College of Life Science, National Taiwan University, Taipei 10617, Taiwan. Electronic address: snchen@ntu.edu.tw.
authors.forename: Shiu-Nan
authors.initials: SN
authors.lastname: Chen
mesh_terms: D000818:Animals; D023681:Cichlids / Q000235:genetics / Q000502:physiology*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D029941:Fish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006498:Hepatectomy; D022781:Hepatocytes / Q000201:enzymology; D006861:Hydrogen Peroxide / Q000378:metabolism; D008099:Liver / Q000502:physiology*; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*; D015027:Zebrafish / Q000502:physiology*
chemical_list: D004791:Enzyme Inhibitors; D029941:Fish Proteins; D009861:Onium Compounds; C007517:diphenyleneiodonium; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases
keywords: Histopathology; Hydrogen peroxide; NADPH oxidase; Partial hepatectomy; Repair