title: Analysis of the chromosomal aneuploidy by interphase fluorescence in situ hybridization (FISH) in Squamous cell carcinoma of the cervix in Jammu region of J and K state.
abstract: OBJECTIVE
  The present study was aimed to analyze the chromosomal changes obtained by using FISH. Fluorescence in situ hybridization (FISH) is a powerful technique used in the detection of chromosomal abnormalities.
  
  
  METHODS
  During the present study, FISH technique was carried out in 26 cases of Squamous cell carcinoma of the cervix using Centromere Enumeration Probes, CEP for chromosome 3 and 17. Statistical tests including ANOVA and Chi square tests were used to analyze the numerical chromosome aberration data obtained by FISH analysis. FISH technique was also carried out in 10 healthy samples (control) by using both CEP 3 and CEP 17 probe.
  
  
  CONCLUSIONS
  FISH results of CEP 3 in the present study showed the frequent gains of chromosome 3 in Squamous cell carcinoma over the chromosome 17. ANOVA results were found to be significant for both CEP 3 and CEP-17 in Squamous cell carcinoma of the cervix. Chi square test (disease v/s control) was also used to study the significant difference between the patients and the controls and it was found to be significant for both chromosomes 3 and 17.
authors.forename: Roopali
authors.initials: R
authors.lastname: Fotra
authors.forename: Shashi
authors.initials: S
authors.lastname: Gupta
authors.forename: Sumit
authors.initials: S
authors.lastname: Koul
authors.affiliation: Human Genetic Research Cum Counseling Centre, Govt. Medical College/University of Jammu, Jammu, India.
authors.forename: Subash
authors.initials: S
authors.lastname: Gupta
mesh_terms: D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000473:pathology; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007194:India; D007399:Interphase; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes