title: Toward enantioselective nano ultrahigh-performance liquid chromatography with Whelk-O1 chiral stationary phase.
abstract: In this study, a Whelk-O1 chiral stationary phase immobilized on 2.5 μm silica particles was employed in nanoLC. Two nanocolumns (180 and 250 mm long, 75 μm id) with a single polymeric organic monolithic outlet frit were packed under high-pressure ultrasonic-assisted packing procedure. The monolithic outlet frit was prepared by thermal polymerization of methacrylate-based monomers affording high-mechanical stability and high-pressure resistance. Very efficient enantioseparations with more than 70 000 plates/m were achieved in normal phase mode by eluting (+/-) acenaphthenol. Nanocolumns were also tested in RP mode by using on-line MS detection with nano-spray ESI ion source. Kinetic performances of columns in RP mode were comparable to those in normal phase-conditions.
authors.affiliation: Department of Drug Chemistry and Technology, "Sapienza" University of Rome, Rome, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Ciogli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pierri
authors.forename: Dorina
authors.initials: D
authors.lastname: Kotoni
authors.forename: Alberto
authors.initials: A
authors.lastname: Cavazzini
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Botta
authors.forename: Claudio
authors.initials: C
authors.lastname: Villani
authors.forename: Jelena
authors.initials: J
authors.lastname: Kocergin
authors.forename: Francesco
authors.initials: F
authors.lastname: Gasparrini
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation*; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D010616:Phenanthrenes / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: C584868:(S,S)-whelk-O1; D004364:Pharmaceutical Preparations; D010616:Phenanthrenes
keywords: Enantioselective near-UHPLC; HRMS detection; Nano chiral columns