title: Structural elucidation of the exopolysaccharide produced by the mangrove fungus Penicillium solitum.
abstract: A water soluble extracellular polysaccharide, designated GW-12, was obtained from the liquid culture broth of the mangrove fungus Penicillium solitum by ethanol precipitation, anion-exchange and size-exclusion chromatography. Reversed-phase high performance liquid chromatography analysis showed that GW-12 mainly consisted of d-mannose, and its molecular weight was estimated to be about 11.3 kDa determined by high performance gel permeation chromatography. On the basis of chemical and spectroscopic analyses, including methylation analysis and nuclear magnetic resonance (NMR) spectroscopy, the structure of GW-12 may be represented as a mannan with branches. The main chain of GW-12 was composed of (1 → 2)-linked α-D-mannopyranose and (1 → 6)-linked α-D-mannopyranose residues, branched by single α-d-mannopyranose units attached to the main chain at C-6 positions of (1 → 2)-linked α-D-mannopyranose residues. There was three branch points for every seven sugar residues in the backbone.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Mengxia
authors.initials: M
authors.lastname: Yan
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China. Electronic address: wenjunmqd@hotmail.com.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Mao
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Xianglan
authors.initials: X
authors.lastname: Kong
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Qianqun
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Baofeng
authors.initials: B
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Shuyao
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
mesh_terms: D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008358:Mannose / Q000032:analysis / Q000302:isolation & purification; D008745:Methylation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010407:Penicillium / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D008351:Mannans; D011134:Polysaccharides; D008358:Mannose
keywords: Extracellular polysaccharide; Mangrove fungus; NMR; Penicillium solitum; Structure