title: Biological characteristics of rat dorsal root ganglion cell and human vascular endothelial cell in mono- and co-culture.
abstract: This study aimed to evaluate the biological activity of rat dorsal root ganglion cell (DRGC) and human vascular endothelial cell (HMVEC) in mono- and co-culture. Expression levels of vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) mRNA were measured by quantitative real-time RT-PCR (qRT-PCR). Western blot analysis was used to identify VEGF and NGF protein expressions. Cell injury was assessed by measuring cell viability with methylthiazol tetrazolium (MTT) assay. The results showed that VEGF and NGF mRNA levels in the HMVEC+DRGC group were significantly higher than those in the DRGC and HMVEC groups (all p < 0.05). There were also greater increases in both VEGF and NGF protein expressions in the HMVEC+DRGC group than those in the DRGC and HMVEC groups (all p < 0.05). The results of MTT analysis revealed significant differences in cell viability among the HMVEC+DRGC group and the DRGC and HMVEC groups (all p < 0.05). In summary, our findings provide evidence that DRGC and HMVEC in co-culture may exhibit greater biological activity than DRGC in mono-culture.
authors.affiliation: Department of Orthopedics, Shengjing Hospital, China Medical University, San Hao Street No. 36, Heping District, Shenyang, 110004, People's Republic of China, yuanquanyq127@163.com.
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Li
authors.forename: Chun-Hou
authors.initials: CH
authors.lastname: An
authors.forename: Li
authors.initials: L
authors.lastname: Sun
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D020932:Nerve Growth Factor