title: A rapid and sensitive LC-MS/MS method for the determination of Pulsatilla saponin D in rat plasma and its application in a rat pharmacokinetic and bioavailability study.
abstract: A simple, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of Pulsatilla saponin D, a potential antitumor constituent isolated from Pulsatilla chinensis in rat plasma. Rat plasma samples were pretreated by protein precipitation with methanol. The method validation was performed in accordance with US Food and Drug Administration guidelines and the results met the acceptance criteria. The method was successfully applied to assess the pharmacokinetics and oral bioavailability of Pulsatilla saponin D in rats.
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, 56 Yangmin Road, Nanchang, 330006, China; Guangzhou University of Chinese Medicine, 232 Waihuandong Road, Guangzhou, 510405, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ouyang
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Guo
authors.forename: Mingzhen
authors.initials: M
authors.lastname: He
authors.forename: Jinlian
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Hongliang
authors.initials: H
authors.lastname: Jiang
authors.forename: Yulin
authors.initials: Y
authors.lastname: Feng
authors.forename: Shilin
authors.initials: S
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000493:pharmacokinetics; D001682:Biological Availability; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000493:pharmacokinetics; D031946:Pulsatilla / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012503:Saponins / Q000008:administration & dosage / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014481:United States; D014486:United States Food and Drug Administration
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C000601508:Pulsatilla saponin D; D012503:Saponins; C084875:saponin D, hupehensis; D009828:Oleanolic Acid
keywords: LC-MS/MS; Pulsatilla saponin D; bioavailability; pharmacokinetics