title: Spectrofluorimetric analysis of famotidine in pharmaceutical preparations and biological fluids by derivatization with benzoin.
abstract: A sensitive and simple spectrofluorimetric method has been developed for the analysis of famotidine, from pharmaceutical preparations and biological fluids after derivatization with benzoin. The reaction was carried out in alkaline medium with measurement of fluorescence intensity at 446 nm with excitation wavelength at 286 nm. Linear calibration was obtained with 0.5-15 μg/ml with coefficient of determination (r(2)) 0.997. The factors affecting the fluorescence intensity were optimized. The pharmaceutical additives and amino acid did not interfere in the determination. The mean percentage recovery (n=4) calculated by standard addition from pharmaceutical preparation was 94.8-98.2% with relative standard deviation (RSD) 1.56-3.34% and recovery from deproteinized spiked serum and urine of healthy volunteers was 98.6-98.9% and 98.0-98.4% with RSD 0.34-0.84% and 0.29-0.87% respectively.
authors.affiliation: Institute of Advance Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan. Electronic address: aalam012@gmail.com.
authors.forename: Malik
authors.initials: M
authors.lastname: Alamgir
authors.affiliation: Institute of Advance Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan.
authors.forename: Muhammad Yar
authors.initials: MY
authors.lastname: Khuhawar
authors.affiliation: M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro, Pakistan.
authors.forename: Saima Q
authors.initials: SQ
authors.lastname: Memon
authors.affiliation: Institute of Advance Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan.
authors.forename: Amir
authors.initials: A
authors.lastname: Hayat
authors.affiliation: Institute of Advance Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan.
authors.forename: Rizwan Ali
authors.initials: RA
authors.lastname: Zounr
mesh_terms: D001573:Benzoin / Q000737:chemistry*; D015738:Famotidine / Q000032:analysis / Q000097:blood* / Q000652:urine*; D006635:Histamine H2 Antagonists / Q000032:analysis / Q000097:blood* / Q000652:urine*; D006801:Humans; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D006635:Histamine H2 Antagonists; D015738:Famotidine; D001573:Benzoin
keywords: Benzoin; Deproteinized; Derivatization; Famotidine; Serum and urine