title: Layer-by-layer assembly of chitosan stabilized multilayered liposomes for paclitaxel delivery.
abstract: Paclitaxel (PTX) loaded multilayered liposomes were prepared using layer-by-layer assembly in an effort to improve the stabilization of the liposomal compositions for PTX delivery. Stearyl amine was used to provide positive charge to the PTX-liposomes, and subsequently coated with anionic polyacrylic acid (PAA) followed by cationic chitosan. Various process variables were optimized and the optimum formulation was found to have particle size of 215 ± 17 nm, zeta potential of +27.9 ± 3.4 mV and encapsulation efficiency of 70.93 ± 2.39%. The lyophilized chitosan-PAA-PTX-liposomes formulation was stable in simulated gastrointestinal fluids and at different environmental conditions (4 °C and 25 °C). In vitro drug release experiments demonstrated that chitosan-PAA-PTX-liposomes formulation exhibited obvious sustained release behaviors compared to PTX-liposomes. Furthermore, chitosan-PAA-PTX-liposomes formulation revealed enhanced PTX induced cytotoxicity in human cervical cancer cell culture experiments compared to PTX-liposomes. In conclusion, the approach presented herein will provide a promising solution for PTX delivery.
authors.affiliation: School of Pharmacy, Anhui University of Chinese Medicine, Meishan Road, Hefei 230038, PR China.
authors.forename: Meng-Xia
authors.initials: MX
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Anhui University of Chinese Medicine, Meishan Road, Hefei 230038, PR China.
authors.forename: Bai-Kun
authors.initials: BK
authors.lastname: Li
authors.affiliation: School of Pharmacy, Anhui University of Chinese Medicine, Meishan Road, Hefei 230038, PR China.
authors.forename: Deng-Ke
authors.initials: DK
authors.lastname: Yin
authors.affiliation: School of Pharmacy, Anhui University of Chinese Medicine, Meishan Road, Hefei 230038, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: School of Pharmacy, Anhui University of Chinese Medicine, Meishan Road, Hefei 230038, PR China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Li
authors.affiliation: School of Pharmacy, Anhui University of Chinese Medicine, Meishan Road, Hefei 230038, PR China. Electronic address: pengdy@ahtcm.edu.cn.
authors.forename: Dai-Yin
authors.initials: DY
authors.lastname: Peng
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry* / Q000633:toxicity; D002784:Cholesterol / Q000737:chemistry; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D008081:Liposomes; D017239:Paclitaxel / Q000008:administration & dosage*; D010743:Phospholipids / Q000737:chemistry; D013696:Temperature; D002583:Uterine Cervical Neoplasms / Q000628:therapy*
chemical_list: D000180:Acrylic Resins; D000972:Antineoplastic Agents, Phytogenic; D008081:Liposomes; D010743:Phospholipids; C006903:carbopol 940; D048271:Chitosan; D002784:Cholesterol; D017239:Paclitaxel
keywords: Chitosan; Layer-by-layer assembly; Liposomes; Paclitaxel