title: Simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in Swertia mussotii Franch by capillary zone electrophoresis with running buffer modifier.
abstract: The method of capillary zone electrophoresis (CZE) with direct UV detection was developed for the determination of oleanolic acid, ursolic acid, quercetin and apigenin. and then for the first time successfully applied to the analysis of four analytes in Swertia mussotii Franch and its preparations. Various factors affecting the CZE procedure were investigated and optimized, and the optimal conditions were: 50 × 10(-3) mol/L borate-phosphate buffer (pH 9.5) with 5.0 × 10(-3) mol/L β-cyclodextrin, 15 kV separation voltage, 20 °C column temperature, 250 nm detection wavelength and 5 s electrokinetic injection time (voltage 20 psi). Under the conditions, oleanolic acid, ursolic acid, quercetin and apigenin could be determined within the test ranges with a good correlation coefficient (r(2) > 0.9991). The limits of detection for conditions, oleanolic acid, ursolic acid, quercetin and apigenin were 0.3415, 0.2003, 0.0062 and 0.2538 µg/mL, respectively, and the intra- and inter-day relative standard deviations were no more than 4.72%. This procedure provided a convenient, sensitive and accurate method for simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in S. mussotii Franch.
authors.affiliation: Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province, Department of Pharmacy, Gansu College of Traditional Chinese Medicine, Lanzhou, 730000, People's Republic of China.
authors.forename: Ruibin
authors.initials: R
authors.lastname: Gao
authors.forename: Litao
authors.initials: L
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Jingman
authors.initials: J
authors.lastname: Ni
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Shuqing
authors.initials: S
authors.lastname: Dong
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
mesh_terms: D047310:Apigenin / Q000032:analysis*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D009828:Oleanolic Acid / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000032:analysis*; D015203:Reproducibility of Results; D029967:Swertia / Q000737:chemistry*; D013607:Tablets / Q000032:analysis; D013997:Time Factors; D014315:Triterpenes / Q000032:analysis*; D014466:Ultraviolet Rays; D047392:beta-Cyclodextrins / Q000737:chemistry; D000097245:Ursolic Acid
chemical_list: D047310:Apigenin; D002021:Buffers; D009828:Oleanolic Acid; D011794:Quercetin; D013607:Tablets; D014315:Triterpenes; D047392:beta-Cyclodextrins; D000097245:Ursolic Acid; C031215:betadex
keywords: CZE; Swertia mussotii Franch; flavonoids; pentacyclic triterpenoids; β- cyclodextrin