title: Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae.
abstract: A novel Gram-stain-negative, facultatively anaerobic, catalase- and oxidase-positive, non-motile and pink-pigmented bacterium, designated G22(T), was isolated from Gahai, a saltwater lake in Qinghai province, China. Optimal growth occurred at 33-35 °C, pH 7.0-7.5, and in the presence of 2-4% (w/v) NaCl. The DNA G+C content was 40.0 mol%. The major polar lipids were phosphatidylethanolamine and three unknown lipids. The predominant cellular fatty acids were iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and iso-C15:0 3-OH, and MK-7 was the main respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain G22(T) fell within the class Bacteroidia. Its closest phylogenetic neighbour was the recently described species Draconibacterium orientale, the sole member of the family Draconibacteriaceae, with merely 90.04% sequence similarity. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence observed, a novel species in a new genus, Tangfeifania diversioriginum gen. nov., sp. nov., is proposed within the family Draconibacteriaceae. The type strain is G22(T) ( = CICC 10587(T) =DSM 27063(T)).
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Liu
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Li
authors.affiliation: National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA.
authors.forename: Alejandro P
authors.initials: AP
authors.lastname: Rooney
authors.affiliation: State key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.|College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
authors.forename: Zong-Jun
authors.initials: ZJ
authors.lastname: Du
authors.affiliation: State key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.|College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Chen
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7