title: Genome sequencing, annotation and comparative genomic analysis of Shigella dysenteriae strain SD1D.
abstract: BACKGROUND
  Shigellosis is an acute form of gastroenteritis caused by the bacteria belonging to the genus Shigella. It is the most common cause of morbidity and mortality in children. Shigella belongs to the family Enterobactericeae, which is a Gram-negative and rod shaped bacterium. In the present study, we report the draft genome of Shigella dysenteriae strain SD1D, which was isolated from the stool sample of a healthy individual.
  
  
  RESULTS
  Based on 16S rRNA gene sequence and phylogenetic analysis, the strain SD1D was identified as Shigella dysenteriae. The draft genome of SD1D consisted of 45, 93, 159 bp with a G + C content of 50.7%, 4, 960 predicted CDSs, 75 tRNAs and 2 rRNAs. The final assembly contained 146 contigs of total length 45, 93, 159 bp with N50 contig length of 77, 053 bp; the largest contig assembled measured 3, 85, 550 bp.
  
  
  CONCLUSIONS
  We have for the first time performed the whole genome sequencing of Shigella dysenteriae strain SD1D. The comparative genomic analysis revealed several genes responsible for the pathogenesis, virulence, defense, resistance to antibiotics and toxic compounds, multidrug resistance efflux pumps and other genomic features of the bacterium.
authors.affiliation: Microbial Type Culture Collection and Gene bank, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
authors.forename: Gurwinder
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Immunology Laboratory, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
authors.forename: Sathyaseelan
authors.initials: S
authors.lastname: Sathyabama
authors.affiliation: Microbial Type Culture Collection and Gene bank, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Arora
authors.affiliation: Immunology Laboratory, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
authors.forename: Sheenam
authors.initials: S
authors.lastname: Verma
authors.affiliation: Immunology Laboratory, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
authors.forename: Nida
authors.initials: N
authors.lastname: Mubin
authors.affiliation: Immunology Laboratory, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
authors.forename: Javed N
authors.initials: JN
authors.lastname: Agrewala
authors.affiliation: Microbial Type Culture Collection and Gene bank, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Mayilraj
keywords: EzTaxon; G + C content; RAST; Shigella dysenteriae; Shigellosis