title: Screening and selection of synthetic peptides for a novel and optimized endotoxin detection method.
abstract: The current validated endotoxin detection methods, in spite of being highly sensitive, present several drawbacks in terms of reproducibility, handling and cost. Therefore novel approaches are being carried out in the scientific community to overcome these difficulties. Remarkable efforts are focused on the development of endotoxin-specific biosensors. The key feature of these solutions relies on the proper definition of the capture protocol, especially of the bio-receptor or ligand. The aim of the presented work is the screening and selection of a synthetic peptide specifically designed for LPS detection, as well as the optimization of a procedure for its immobilization onto gold substrates for further application to biosensors.
authors.affiliation: CEIT-IK4 and Tecnun, University of Navarra, Manuel de Lardizabal 15, 20018 San Sebastian, Spain. Electronic address: mmujika@ceit.es.
authors.forename: M
authors.initials: M
authors.lastname: Mujika
authors.affiliation: CEIT-IK4 and Tecnun, University of Navarra, Manuel de Lardizabal 15, 20018 San Sebastian, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Zuzuarregui
authors.affiliation: Department of Microbiology and Parasitology, University of Navarra, Irunlarrea 1, E-31008, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Sánchez-Gómez
authors.affiliation: Department of Microbiology and Parasitology, University of Navarra, Irunlarrea 1, E-31008, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Martínez de Tejada
authors.affiliation: CEIT-IK4 and Tecnun, University of Navarra, Manuel de Lardizabal 15, 20018 San Sebastian, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Arana
authors.affiliation: CEIT-IK4 and Tecnun, University of Navarra, Manuel de Lardizabal 15, 20018 San Sebastian, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Pérez-Lorenzo
mesh_terms: D001419:Bacteria; D015374:Biosensing Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000032:analysis* / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D055767:Immobilized Proteins; D008070:Lipopolysaccharides; D010455:Peptides
keywords: LPS; Peptide; QCM