title: Potential role of circulating microRNAs as early markers of preeclampsia.
abstract: OBJECTIVE
  To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum of pregnant women, who later developed severe preeclampsia (sPE) in the third trimester of pregnancy (n = 24) compared to women with normal pregnancy (n = 24).
  
  
  METHODS
  Sera from 12-14-week-gestation whole blood were subjected to microarray analysis with TaqMan Low Density Array chips (human microRNA panel V3.0), and to quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  By using the TaqMan Low Density Array chip technology, 19 mature miRNAs appeared differentially expressed in the group of women who later developed sPE as compared to normal women. The expression of four miRNAs (miR-1233, miR-520, miR-210, miR-144) was validated by quantitative real-time polymerase chain reaction analysis. MiR-1233 was the most overexpressed in the serum of women who later developed sPE.
  
  
  CONCLUSIONS
  Circulating miRNAs deserve further investigation in order to explore their potential role in the pathogenesis of preeclampsia. In particular, miR-1233 might represent a potential marker of early sPE.
authors.affiliation: Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, Italy. Electronic address: blendi.ura2006@libero.it.
authors.forename: Blendi
authors.initials: B
authors.lastname: Ura
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
authors.forename: Giordana
authors.initials: G
authors.lastname: Feriotto
authors.affiliation: Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Monasta
authors.affiliation: Higher Institute of Technological Studies, University of Bizerte, Bizerte, Tunisia.
authors.forename: Sabrine
authors.initials: S
authors.lastname: Bilel
authors.affiliation: Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Zweyer
authors.affiliation: Department of Life Sciences, University of Trieste, Trieste, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Celeghini
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D005260:Female; D005865:Gestational Age; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000097:blood*; D020411:Oligonucleotide Array Sequence Analysis; D010865:Pilot Projects; D011225:Pre-Eclampsia / Q000097:blood* / Q000235:genetics*; D011247:Pregnancy; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; C000597075:MIRN-1233 microRNA, human; C554293:MIRN144 microRNA, human; C543800:MIRN210 microRNA, human; C575048:MIRN520 microRNA, human; D035683:MicroRNAs
keywords: early biomarkers; microRNA; preeclampsia