title: A dynamic interface between vacuoles and mitochondria in yeast.
abstract: Cellular life depends on continuous transport of lipids and small molecules between mitochondria and the endomembrane system. Recently, endoplasmic reticulum-mitochondrial encounter structure (ERMES) was identified as an important yet nonessential contact for such transport. Using a high-content screen in yeast, we found a contact site, marked by Vam6/Vps39, between vacuoles (the yeast lysosomal compartment) and mitochondria, named vCLAMP (vacuole and mitochondria patch). vCLAMP is enriched with ion and amino-acid transporters and has a role in lipid relay between the endomembrane system and mitochondria. Critically, we show that mitochondria are dependent on having one of two contact sites, ERMES or vCLAMP. The absence of one causes expansion of the other, and elimination of both is lethal. Identification of vCLAMP adds to our ability to understand the complexity of interorganellar crosstalk.
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Yael
authors.initials: Y
authors.lastname: Elbaz-Alon
authors.affiliation: Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Eden
authors.initials: E
authors.lastname: Rosenfeld-Gur
authors.affiliation: Electron Microscopy Unit, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Vera
authors.initials: V
authors.lastname: Shinder
authors.affiliation: Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Anthony H
authors.initials: AH
authors.lastname: Futerman
authors.affiliation: Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel.
authors.forename: Tamar
authors.initials: T
authors.lastname: Geiger
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel. Electronic address: maya.schuldiner@weizmann.ac.il.
authors.forename: Maya
authors.initials: M
authors.lastname: Schuldiner
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D001692:Biological Transport; D002468:Cell Physiological Phenomena; D002853:Chromatography, Liquid; D004721:Endoplasmic Reticulum / Q000378:metabolism; D047468:Immunoprecipitation; D008561:Membrane Fusion / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis*; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D010743:Phospholipids; D029701:Saccharomyces cerevisiae Proteins; C406817:VAM6 protein, S cerevisiae