title: Type III polyketide synthase is involved in the biosynthesis of protocatechuic acid in Aspergillus niger.
abstract: Genomic studies have shown that not only plants but also filamentous fungi contain type III polyketide synthases. To study the function of type III polyketide synthase (AnPKSIII) in Aspergillus niger, a deletion strain (delAnPKSIII) and an overexpression strain (oeAnPKSIII) were constructed in A. niger MA169.4, a derivative of the wild-type (WT) A. niger ATCC 9029 that produces large quantities of gluconic acid. Alterations in the metabolites were analyzed by HPLC when the extract of the overexpression strain was compared with extracts of the WT and deletion strains. Protocatechuic acid (PCA; 3,4-dihydroxybenzoic acid, 3.2 mg/l) was isolated and identified as the main product of AnPKSIII when inductively expressed in A. niger MA169.4. The molecular weight of PCA was 154.1 (m/z 153.1 [M-H](-)), was detected by ESI-MS in the negative ionization mode, and (1)H and (13)C NMR data confirmed its structure.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, People's Republic of China.
authors.forename: Yangyong
authors.initials: Y
authors.lastname: Lv
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.forename: Li
authors.initials: L
authors.lastname: Pan
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D062385:Hydroxybenzoates / Q000032:analysis / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D062385:Hydroxybenzoates; C009091:protocatechuic acid; D000217:Acyltransferases; C020141:flavanone synthetase