title: Sub-chronic lead and cadmium co-induce apoptosis protein expression in liver and kidney of rats.
abstract: The combined subchronic effects of exposure to lead acetate and cadmium chloride on apoptosis protein expression were detected in the liver and kidney of rats to investigate the hazards of environmentally relevant, low-dose exposure to these compounds. The TUNEL assay showed that there were increased numbers of apoptotic cells. Immunohistochemical tests showed increased numbers of positive cells under Bax and caspase-3 protein detection and decreased Bcl-2 protein. Furthermore, mitochondrial injury and increased numbers of apoptotic cells with condensed nuclei were observed by TEM. These results suggested that low-dose exposure to Pb and Cd can cause significant hepatic and renal apoptosis and finally impair their function. Hepatic and renal apoptosis induced by low-dose exposure is associated with mitochondrial injury and changes in levels of apoptogenic proteins, such as Bcl-2, Bax, and caspase-3.
authors.affiliation: Analytical & Testing Center, Sichuan University Chengdu 610065, China.
authors.forename: Guiping
authors.initials: G
authors.lastname: Yuan
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China ; TONGWEI CO., LTD. Chengdu 610041, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Dai
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Zhongqiong
authors.initials: Z
authors.lastname: Yin
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Hongke
authors.initials: H
authors.lastname: Lu
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Renyong
authors.initials: R
authors.lastname: Jia
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shu
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Xinghong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Qiaojia
authors.initials: Q
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Changliang
authors.initials: C
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Lizi
authors.initials: L
authors.lastname: Yin
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University Ya'an 625014, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lv
authors.affiliation: TONGWEI CO., LTD. Chengdu 610041, China.
authors.forename: Qiaobo
authors.initials: Q
authors.lastname: Lei
authors.affiliation: TONGWEI CO., LTD. Chengdu 610041, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: TONGWEI CO., LTD. Chengdu 610041, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Mi
authors.affiliation: TONGWEI CO., LTD. Chengdu 610041, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Yu
authors.affiliation: TONGWEI CO., LTD. Chengdu 610041, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D019256:Cadmium Chloride / Q000633:toxicity*; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D046529:Microscopy, Electron, Transmission; D009942:Organometallic Compounds / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009942:Organometallic Compounds; D019256:Cadmium Chloride; C008261:lead acetate
keywords: Bcl-2; Pb and Cd; apoptosis; bax; caspase-3; subchronic