title: Effect of alginate and chitosan on viability and release behavior of Bifidobacterium pseudocatenulatum G4 in simulated gastrointestinal fluid.
abstract: The main aim of this study was to investigate the effect of different coating materials (i.e. Na-alginate and chitosan) on the viability and release behavior of Bifidobacterium pseudocatenulatum G4 in the simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). This study reports the viability of encapsulated B. pseudocatenulatum G4 coated using different alginate (2-4 g/100mL) and chitosan (0.2-0.8 g/100mL) concentrations. The results indicated that the highest concentration of alginate (4.4142 g/100mL) along with 0.5578 g/100mL chitosan resulted in the highest viability of B. pseudocatenulatum G4. The release behavior of the encapsulated probiotics in SGF (pH 1.5) in 2h followed by 4h in SIF (pH 7.4) was also assessed. The resistance rate of alginate-chitosan capsule in SGF was higher than SIF. The alginate-chitosan encapsulated cells had also more resistance than alginate capsules. The current study revealed that alginate encapsulated B. Pseudocatenulatum G4 exhibited longer survival than its free cells (control).
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia (UPM), 43400, UPM Serdang, Malaysia. Electronic address: nikikamalian@gmail.com.
authors.forename: Nikoo
authors.initials: N
authors.lastname: Kamalian
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia (UPM), 43400, UPM Serdang, Malaysia.
authors.forename: Hamed
authors.initials: H
authors.lastname: Mirhosseini
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400, UPM Serdang, Malaysia.
authors.forename: Shuhaimi
authors.initials: S
authors.lastname: Mustafa
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia (UPM), 43400, UPM Serdang, Malaysia.
authors.forename: Mohd Yazid Abd
authors.initials: MY
authors.lastname: Manap
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001644:Bifidobacterium / Q000166:cytology* / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D002214:Capsules; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry*; D004339:Drug Compounding; D005750:Gastric Juice / Q000382:microbiology*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007419:Intestinal Secretions / Q000382:microbiology*; D050296:Microbial Viability; D019936:Probiotics / Q000008:administration & dosage* / Q000378:metabolism
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Alginate; Cell viability; Chitosan; Encapsulation; Probiotics; Release behavior