title: Progesterone receptor B promoter hypermethylation in human placenta after labor onset.
abstract: To determine the methylation status of progesterone receptor B (PR-B) promoter and how PR-B regulates progesterone action in placenta during human pregnancy. Placentas were obtained from the pregnancy women at term who underwent cesarean delivery and vaginal delivery. The methylation status of the PR-B promoter was analyzed using the methylation-specific polymerase chain reaction (PCR) and bisulfite sequencing PCR. And the messenger RNA (mRNA) and protein expression of the PR-B and DNA methyltransferases (DNMTs) were determined by quantitative real-time PCR and Western blot. Compared with the cesarean group, the placentas of vaginal delivery group had greater levels of PR-B DNA methylation, and the PR-B, DNMT1, DNMT3a, and DNMT3b mRNA and protein expression were significantly decreased. Progesterone receptor B methylation occurs with high frequency after labor onset and may play an important epigenetic mechanism of labor-related PR-B negative expression, thereby mediating the biological process of functional progesterone withdrawal at term for parturition.
authors.affiliation: Division of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Division of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cui
authors.affiliation: Division of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning, China.
authors.forename: Sishi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Division of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning, China.
authors.forename: Dongming
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Division of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning, China liucx0109@sina.com.
authors.forename: Caixia
authors.initials: C
authors.lastname: Liu
mesh_terms: D000328:Adult; D015153:Blotting, Western; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D000090205:DNA Methyltransferase 3A; D044127:Epigenesis, Genetic; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007743:Labor, Obstetric / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011980:Receptors, Progesterone / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097584:DNA Methyltransferase 3B
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D000097584:DNA Methyltransferase 3B; C000717967:DNMT3A protein, human; C097314:progesterone receptor B; C000619380:DNMT1 protein, human
keywords: DNA methylation; DNA methyltransferases; human parturition; placenta; progesterone receptor B