title: Antitumor metastasis activity of pectic polysaccharide purified from the peels of Korean Citrus Hallabong.
abstract: A polysaccharide fraction, HBE-III, was successfully purified in a high yield (40.4%) from its crude polysaccharide (HBE-0) which was prepared from pectinase hydrolysates of the peels of the Korean Citrus Hallabong. In experimental lung metastasis studies of Colon 26-M3.1 carcinoma cells, prophylactic administration of HBE-III significantly inhibited lung metastasis in a dose-dependent manner. In an in vitro cytotoxicity analysis, HBE-III (up to 1000 μg/mL) did not affect the growth of Colon 26-M3.1 cells and normal cells. HBE-III enhanced production of IL-6 and IL-12 by murine peritoneal macrophages. In an assay for natural killer (NK) cell activity, HBE-III (1000 μg/mouse, i.v.) significantly augmented NK cytotoxicity against Yac-1 tumor cells. The depletion of NK cells by injection of mouse anti-asialo GM1 serum abolished the inhibitory effect of HBE-III on lung metastasis of Colon 26-M3.1 cells. These data suggest that HBE-III may inhibit tumor metastasis via activation of macrophages and NK cells.
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, San 94-6, Ieui-dong, Youngtong-gu, Suwon, Gyeonggi-do 443-760, Republic of Korea.
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Lee
authors.affiliation: Department of Integrated Biomedical and Life Science, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Hye-Ryung
authors.initials: HR
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, San 94-6, Ieui-dong, Youngtong-gu, Suwon, Gyeonggi-do 443-760, Republic of Korea.
authors.forename: Myoung-Sook
authors.initials: MS
authors.lastname: Shin
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, San 94-6, Ieui-dong, Youngtong-gu, Suwon, Gyeonggi-do 443-760, Republic of Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Cho
authors.affiliation: BK-Bio Co. Ltd., 5439-1, Sangdaewon-dong, Jungwon-gu, Sungnam, Gyeonggi-do 462-819, Republic of Korea.
authors.forename: Hyuk-Joon
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, San 94-6, Ieui-dong, Youngtong-gu, Suwon, Gyeonggi-do 443-760, Republic of Korea. Electronic address: ksshin@kyonggi.ac.kr.
authors.forename: Kwang-Soon
authors.initials: KS
authors.lastname: Shin
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D002460:Cell Line; D045744:Cell Line, Tumor; D002957:Citrus / Q000737:chemistry*; D003106:Colon / Q000276:immunology / Q000473:pathology; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology*; D005260:Female; D006868:Hydrolysis; D018664:Interleukin-12 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology / Q000517:prevention & control* / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D015850:Interleukin-6; D011134:Polysaccharides; C042492:rhamnogalacturonan II; D018664:Interleukin-12; D010368:Pectins
keywords: Antimetastasis activity; Citrus Hallabong peel; Natural killer cell; Polysaccharide