title: Comprehensive proteomic analysis of a Chinese 2-herb formula (Astragali Radix and Rehmanniae Radix) on mature endothelial cells.
abstract: Endothelial cells are crucially involved in wound healing angiogenesis, restoring blood flow to wound tissues. Our previous study demonstrated that the Chinese 2-herb formula (NF3) possesses significant wound healing effect in diabetic foot ulcer rats with promising in vitro proangiogenic effects on human umbilical vein endothelial cells (HUVEC). Here, we present the comparative global proteome analysis of NF3-treated HUVEC in static or scratch conditions, screening the comprehensive molecular targets in governing the proangiogenic response in wound healing. Our results suggest plasminogen activator inhibitor-1, specifically down-regulated in static condition and Annexin A1 and Annexin A2, up-regulated in scratch condition, as principal proteins responsible for the proangiogenesis in wound healing. We also identified a panel of cytoskeleton regulatory proteins in static and scratch condition, mediating the migratory behavior of NF3-treated HUVEC. The key proteins in static state include myosin regulatory light polypeptide 9, SPAST, tropomyosin (TPM)2, and Vimentin while that in scratch state contained prelamin-A/C, TPM1, TPM2, and Vimentin. In addition, NF3 was shown to regulate transcription and translation, cell-cell interaction, and ROS defense in HUVEC. Proliferation and migration assays further confirmed the identified principal proteins plasminogen activator inhibitor-1 and Annexin A2 which are responsible for NF3-induced proangiogenesis of HUVEC in wound healing. This is the first study on the global proteome expression of NF3-treated HUVEC with the identification of the differences at the molecular level, between static and scratch conditions involved in wound healing angiogenesis.
authors.affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
authors.forename: Jacqueline Chor Wing
authors.initials: JC
authors.lastname: Tam
authors.forename: Chun Hay
authors.initials: CH
authors.lastname: Ko
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Ching Po
authors.initials: CP
authors.lastname: Lau
authors.forename: Wai Yee
authors.initials: WY
authors.lastname: Chan
authors.forename: Ping Chung
authors.initials: PC
authors.lastname: Leung
authors.forename: Kwok Pui
authors.initials: KP
authors.lastname: Fung
authors.forename: Jin Fang
authors.initials: JF
authors.lastname: Zhang
authors.forename: Clara Bik San
authors.initials: CB
authors.lastname: Lau
mesh_terms: D027883:Astragalus Plant / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D060066:Protein Interaction Maps / Q000187:drug effects; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000145:classification; D020543:Proteome / Q000032:analysis / Q000187:drug effects*; D040901:Proteomics / Q000379:methods*; D032267:Rehmannia / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D011506:Proteins; D020543:Proteome
keywords: Angiogenesis; Astragali Radix; Biomedicine; Endothelial cells; Rehmanniae Radix; Wound healing