title: Cellular metabolism regulates contact sites between vacuoles and mitochondria.
abstract: Emerging evidence suggests that contact sites between different organelles form central hubs in the coordination of cellular physiology. Although recent work has emphasized the crucial role of the endoplasmic reticulum in interorganellar crosstalk, the cooperative behavior of other organelles is largely unexplored. Here, we identify a contact site named vCLAMP (vacuole and mitochondria patch) that integrates mitochondria with the lysosome-like vacuole and thus the endocytic pathway. vCLAMPs depend on the vacuolar HOPS tethering complex subunit Vps39/Vam6 and the Rab GTPase Ypt7, which also participate in membrane fusion at the vacuole. Intriguingly, vCLAMPs are located proximal to the ER-mitochondria encounter structure (ERMES) complexes, and an increase in vCLAMPs can rescue the growth defect of ERMES mutants. Importantly, the persistence of vCLAMPs is regulated by phosphorylation of Vps39 and is strongly reduced during respiratory growth. The identification of this organelle contact site reveals a physical and metabolic interconnection between the endocytic pathway and mitochondria.
authors.affiliation: Biochemistry Section, Department of Biology/Chemistry, University of Osnabrück, Barbarastrasse 13, 49076 Osnabrück, Germany.
authors.forename: Carina
authors.initials: C
authors.lastname: Hönscher
authors.affiliation: Department of Cell Biology, Center for Molecular Medicine, University Medical Centre Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the Netherlands.
authors.forename: Muriel
authors.initials: M
authors.lastname: Mari
authors.affiliation: Biochemistry Section, Department of Biology/Chemistry, University of Osnabrück, Barbarastrasse 13, 49076 Osnabrück, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Auffarth
authors.affiliation: Institute for Biochemistry and Molecular Biology, ZBMZ, and BIOSS Centre for Biological Signalling Studies, University of Freiburg, Stefan-Meier-Strasse 17, 79104 Freiburg, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Bohnert
authors.affiliation: Department of Cell Biology, Center for Molecular Medicine, University Medical Centre Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the Netherlands.
authors.forename: Janice
authors.initials: J
authors.lastname: Griffith
authors.affiliation: Molecular Cell Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
authors.forename: Willie
authors.initials: W
authors.lastname: Geerts
authors.affiliation: Institute for Biochemistry and Molecular Biology, ZBMZ, and BIOSS Centre for Biological Signalling Studies, University of Freiburg, Stefan-Meier-Strasse 17, 79104 Freiburg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: van der Laan
authors.affiliation: Biochemistry Section, Department of Biology/Chemistry, University of Osnabrück, Barbarastrasse 13, 49076 Osnabrück, Germany.
authors.forename: Margarita
authors.initials: M
authors.lastname: Cabrera
authors.affiliation: Department of Cell Biology, Center for Molecular Medicine, University Medical Centre Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the Netherlands.
authors.forename: Fulvio
authors.initials: F
authors.lastname: Reggiori
authors.affiliation: Biochemistry Section, Department of Biology/Chemistry, University of Osnabrück, Barbarastrasse 13, 49076 Osnabrück, Germany. Electronic address: cu@uos.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Ungermann
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D001692:Biological Transport; D002468:Cell Physiological Phenomena; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D008561:Membrane Fusion; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism*; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D029701:Saccharomyces cerevisiae Proteins; C406817:VAM6 protein, S cerevisiae