title: TGF β1 mediates epithelial mesenchymal transition via β6 integrin signaling pathway in breast cancer.
abstract: BACKGROUND
  To understand the function of β6 integrin and elucidate its signaling pathways in TGF-β-induced EMT in breast cancer.
  
  
  METHODS
  The interactions between TGF-β1 and β6 integrin were measured by coimmunoprecipitation. The EMT responses, phospherlation of PI3K/Akt and COX-2 expression were determined by real-time PCR, transwell assay, and western blot after the blockage of β6 integrin.
  
  
  RESULTS
  TGF-β1 and β6 integrin could bind with each other. Blockage of β6 integrin rescued TGF-β1-induced EMT phenotype and reduced expression of COX-2 via dephosphorylation of PI3K/Akt.
  
  
  CONCLUSIONS
  β6 integrin plays a critical role in TGF-β1-induced EMT and overexpression of COX-2 in breast cancer.
authors.affiliation: Department of Pathology, Weifang Medical University , Weifang, Shandong Province , P.R. China , 1.
authors.forename: Wentong
authors.initials: W
authors.lastname: Li
authors.forename: Baogang
authors.initials: B
authors.lastname: Zhang
authors.forename: Hongli
authors.initials: H
authors.lastname: Li
authors.forename: Chunling
authors.initials: C
authors.lastname: Zhao
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhong
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Shijun
authors.initials: S
authors.lastname: Lv
mesh_terms: D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006801:Humans; D047468:Immunoprecipitation; D039641:Integrin beta Chains / Q000235:genetics / Q000378:metabolism*; D061986:MCF-7 Cells; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D039641:Integrin beta Chains; D053773:Transforming Growth Factor beta1; C072170:integrin beta6; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: Breast carcinoma; COX-2; EMT; PI3K pathways; TGF-β; β6 integrin