title: Leptospiral lipopolysaccharide stimulates the expression of toll-like receptor 2 and cytokines in pig fibroblasts.
abstract: Pigs throughout the world are afflicted with leptospirosis, causing serious economic losses and potential hazards to human health. Although it has been known that leptospiral lipopolysaccharide (L-LPS) is involved in an immunological reaction between an antigen and a host cell, little is known about how the immune system of pigs can respond to L-LPS. Here, we stimulated pig fibroblasts by L-LPS and then quantitatively measured gene and protein expression levels of two toll-like receptors (TLRs), TLR2 and TLR4, by real-time PCR and Western blotting. As a result, expression of TLR2 was found to be significantly up-regulated within 24 h after L-LPS stimulation whereas induction of TLR4 expression was relatively weak. We also revealed that of myeloid differentiation primary response gene 88 (MyD88), interleukin 6 (IL-6) and IL-8 gene expressions were markedly up-regulated by L-LPS stimulation. These results may suggest that the pig cell can activate TLR2 rather than TLR4 by L-LPS stimulation, thereby inducing expression of cytokines.
authors.affiliation: Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Guo
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Fukuda
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Donai
authors.forename: Kengo
authors.initials: K
authors.lastname: Kuroda
authors.forename: Mizuki
authors.initials: M
authors.lastname: Masuda
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoneyama
authors.forename: Emiko
authors.initials: E
authors.lastname: Isogai
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism*; D007921:Leptospira interrogans / Q000737:chemistry; D008070:Lipopolysaccharides / Q000009:adverse effects* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013268:Stimulation, Chemical; D013552:Swine; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018836:Inflammation Mediators; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D053594:Myeloid Differentiation Factor 88; D012333:RNA, Messenger; D051195:Toll-Like Receptor 2
keywords: Leptospiral lipopolysaccharide; pig fibroblasts; toll-like receptor 2