title: Analysis of genetic diversity of certain species of Piper using RAPD-based molecular markers.
abstract: The utility of RAPD markers in assessing genetic diversity and phenetic relationships of six different species of Piper from Northeast India was investigated. Polymerase chain reaction (PCR) with four arbitrary 10-mer oligonucleotide primers applied to the six species produced a total of 195 marker bands, of which, 159 were polymorphic. On average, six RAPD fragments were amplified per reaction. In the UPGMA phenetic dendrogram based on Jaccard's coefficient, the different accessions of Piper showed a high level of genetic variation. This study may be useful in identifying diverse genetic stocks of Piper, which may then be conserved on a priority basis.
authors.affiliation: Department of Botany, B. Borooah College, Guwahati, Assam, India, chowdhury_u@rediffmail.com.
authors.forename: Utpal
authors.initials: U
authors.lastname: Chowdhury
authors.forename: Bhaben
authors.initials: B
authors.lastname: Tanti
authors.forename: Parakkal
authors.initials: P
authors.lastname: Rethy
authors.forename: Padma Raj
authors.initials: PR
authors.lastname: Gajurel
mesh_terms: D017931:DNA Primers / Q000737:chemistry*; D014644:Genetic Variation; D007194:India; D031701:Piper / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D017931:DNA Primers