title: Effects of two sulfated triterpene saponins echinoside A and holothurin A on the inhibition of dietary fat absorption and obesity reduction.
abstract: Two similarly sulfated triterpene saponins from Pearsonothuria graeffei were prepared to investigate the anti-obesity effects of echinoside A (EA) and holothurin A (HA). The in vitro inhibitory activities of EA and HA toward pancreatic lipase were investigated, and two in vivo studies were performed: (i) Male Wistar rats were orally administered the lipid emulsion with or without a saponin (HA or EA). The serum's total triglyceride concentration was measured at various times. (ii) C57BL/6 mice were assigned to four groups, high fat (HF), EA (0.03%), HA (0.04%), and orlistat (0.01%), and the weight of adipose tissue and level of fatty acids excreted in the feces were determined. Both EA and HA repressed the pancreatic lipase activity and increased fatty acid excretion in the feces. Treatment with EA and HA significantly decreased the adipose tissue accumulation in mice. EA and HA manifested different inhibitory activities in vitro, but each of them dramatically inhibited lipid absorption in vivo and showed strong anti-obesity activity.
authors.affiliation: a College of Food Science and Engineering , Ocean University of China , Qingdao , China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiahui
authors.initials: J
authors.lastname: Wang
authors.forename: Ryo C
authors.initials: RC
authors.lastname: Yanagita
authors.forename: Chunhua
authors.initials: C
authors.lastname: Liu
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Hu
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
authors.forename: Yong
authors.initials: Y
authors.lastname: Xue
mesh_terms: D065966:Absorption, Physicochemical / Q000187:drug effects*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004041:Dietary Fats / Q000378:metabolism*; D004435:Eating / Q000187:drug effects; D006697:Holothurin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D008049:Lipase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D062105:Molecular Docking Simulation; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000201:enzymology; D011487:Protein Conformation; D051381:Rats
chemical_list: D004041:Dietary Fats; D006697:Holothurin; C034723:holothurin A; C043973:echinoside A; D008049:Lipase
keywords: echinoside A; holothurin A; lipid absorption; obesity; saponin