title: Putative signal peptides of two BURP proteins can direct proteins to their destinations in tobacco cell system.
abstract: Plant-specific BURP family proteins have a diverse subcellular localization with different functions. However, only limited studies have investigated the functions of their different domains. In the present study, the role of the N-terminal putative signal peptide in protein subcellular localization was investigated using a tobacco cell system. The results showed that SALI3-2 was present in vacuoles, whereas AtRD22 was directed to the apoplast. The N-terminal putative signal peptides of both proteins were confirmed to be the essential and critical domains for targeting the proteins to their destinations. We also demonstrate that the expression and accumulation of mGFP in tobacco cells was increased when mGFP was fused to the putative signal peptide of SALI3-2. The findings offer the potential application of this short peptide in protein production in plants.
authors.affiliation: College of Life Science, Shenzhen University, Shenzhen, 518060, China, yltang@szu.edu.cn.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Tang
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Ou
authors.forename: Jianbin
authors.initials: J
authors.lastname: Qiu
authors.forename: Zilan
authors.initials: Z
authors.lastname: Mi
mesh_terms: D002460:Cell Line; D042541:Intracellular Space / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics*; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D014617:Vacuoles / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins