title: Love-wave sensors combined with microfluidics for fast detection of biological warfare agents.
abstract: The following paper examines a time-efficient method for detecting biological warfare agents (BWAs). The method is based on a system of a Love-wave immunosensor combined with a microfluidic chip which detects BWA samples in a dynamic mode. In this way a continuous flow-through of the sample is created, promoting the reaction between antigen and antibody and allowing a fast detection of the BWAs. In order to prove this method, static and dynamic modes have been simulated and different concentrations of BWA simulants have been tested with two immunoreactions: phage M13 has been detected using the mouse monoclonal antibody anti-M13 (AM13), and the rabbit immunoglobulin (Rabbit IgG) has been detected using the polyclonal antibody goat anti-rabbit (GAR). Finally, different concentrations of each BWA simulants have been detected with a fast response time and a desirable level of discrimination among them has been achieved.
authors.affiliation: Instituto de Tecnologías Físicas y de la Información (ITEFI), CSIC, Serrano 144, Madrid 28006, Spain. daniel.matatagui@ccadet.unam.mx.
authors.forename: Daniel
authors.initials: D
authors.lastname: Matatagui
authors.affiliation: Instituto de Tecnologías Físicas y de la Información (ITEFI), CSIC, Serrano 144, Madrid 28006, Spain. joseluis.fontecha@csic.es.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Fontecha
authors.affiliation: Instituto de Tecnologías Físicas y de la Información (ITEFI), CSIC, Serrano 144, Madrid 28006, Spain. mj.fernandez@csic.es.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Fernández
authors.affiliation: Instituto de Microelectrónica de Barcelona, CSIC, Campus UAB, Bellaterra 08193, Spain. isabel.gracia@csic.es.
authors.forename: Isabel
authors.initials: I
authors.lastname: Gràcia
authors.affiliation: Instituto de Microelectrónica de Barcelona, CSIC, Campus UAB, Bellaterra 08193, Spain. carles.cane@imb-cnm.csic.es.
authors.forename: Carles
authors.initials: C
authors.lastname: Cané
authors.affiliation: Instituto de Tecnologías Físicas y de la Información (ITEFI), CSIC, Serrano 144, Madrid 28006, Spain. jp.santos@csic.es.
authors.forename: José Pedro
authors.initials: JP
authors.lastname: Santos
authors.affiliation: Instituto de Tecnologías Físicas y de la Información (ITEFI), CSIC, Serrano 144, Madrid 28006, Spain. carmen.horrillo.guemes@csic.es.
authors.forename: María Carmen
authors.initials: MC
authors.lastname: Horrillo
mesh_terms: D000162:Acoustics / Q000295:instrumentation*; D000818:Animals; D017104:Bacteriophage M13 / Q000302:isolation & purification*; D054045:Biological Warfare Agents / Q000145:classification*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004784:Environmental Monitoring / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006041:Goats; D007118:Immunoassay / Q000295:instrumentation; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011817:Rabbits; D014159:Transducers
chemical_list: D054045:Biological Warfare Agents