title: Hyperthermia inhibited the migration of tongue squamous cell carcinoma through TWIST2.
abstract: BACKGROUND
  Hyperthermia has been shown promising in the treatment of head and neck squamous cell carcinoma (HNSCC); however, the mechanism underlying hyperthermia reducing tumor metastasis is poorly elucidated. TWIST2, an important transcription factor of epithelial-mesenchymal transition (EMT), plays a critical role in the tumor progression and metastasis. The role of TWIST2 in tongue squamous cell carcinoma (TSCC) and its association with hyperthermia still have not been reported.
  
  
  METHODS
  The correlations between TWIST2 expression and the clinical-pathologic characteristics of 89 patients with TSCC were evaluated by immunohistochemical staining. TSCC cell lines transfected with siRNA against TWIST2 were heated for 40 min at 42.5°C, and the migration capability of cells was examined by migration assay. Xenograft tumors in nude mice were treated by hyperthermia, and TWIST2 expression was measured.
  
  
  RESULTS
  Our data showed that TWIST2 expression was associated with the metastasis of human TSCC. In Tca8113 and Cal-27 cells, TWIST2-siRNA treatment can reduce cell migration ability and has no effect on the cell proliferation and apoptosis. Hyperthermia can decrease the level of TWIST2 in TSCC and inhibit the migration of cells.
  
  
  CONCLUSIONS
  This demonstrated that hyperthermia might decrease the migration of Tca8113 and Cal-27 cells by reducing TWIST2 expression. Altogether, these findings suggest an as yet undescribed link between TWIST2 and hyperthermia in TSCC.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, (Sichuan University), Chengdu, Sichuan, China; Department of Oral Pathology, West China Hospital of Stomatology, (Sichuan University), Chengdu, Sichuan, China.
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Tang
authors.forename: Jian
authors.initials: J
authors.lastname: Jiang
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.forename: Yong-wen
authors.initials: YW
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Yun-long
authors.initials: YL
authors.lastname: Fan
authors.forename: Qian-ming
authors.initials: QM
authors.lastname: Chen
authors.forename: Chu-hang
authors.initials: CH
authors.lastname: Liao
authors.forename: Xin-hua
authors.initials: XH
authors.lastname: Liang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D011897:Random Allocation; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D000077195:Squamous Cell Carcinoma of Head and Neck; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D014162:Transfection; D051879:Twist-Related Protein 1 / Q000096:biosynthesis* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D034741:RNA, Small Interfering; D012097:Repressor Proteins; C486274:TWIST2 protein, human; D051879:Twist-Related Protein 1
keywords: TWIST2; epithelial-mesenchymal transition; hyperthermia; migration