title: Molecular cloning and characterization of pathogenesis-related protein family 10 gene from spinach (SoPR10).
abstract: PR10 genes encode small, intracellular proteins that respond to biotic and abiotic stresses. In this study, a cDNA clone (designated as SoPR10, GenBank Accession No. KC142174) encoding a PR10 protein from spinach (Spinacia oleracea L.) was isolated and characterized. SoPR10 encoded a 161-amino acid polypeptide with a predicted molecular mass of 19.76 kDa and a pI of 4.61. Real-time quantitative analysis indicated that SoPR10 was constitutively expressed in root and shoot. The abundance of SoPR10 in salt-resistant cultivar (Chaoji) was generally greater than in salt-sensitive cultivar (Daye) under 160 mM L(-1) NO3(-) treatment for 0.5, 3, and 6 h. The expression of SoPR10 was also induced by other abiotic stresses including polyethylene glycol, NaCl, salicylic acid, and H2O2. Our results indicated that SoPR10 might play important roles under nitrate stress and other abiotic stresses.
authors.affiliation: a Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming , China.
authors.forename: Xuegui
authors.initials: X
authors.lastname: Bai
authors.forename: Juan
authors.initials: J
authors.lastname: Long
authors.forename: Xiaozhao
authors.initials: X
authors.lastname: He
authors.forename: Shun
authors.initials: S
authors.lastname: Li
authors.forename: Huini
authors.initials: H
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009566:Nitrates / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D011487:Protein Conformation; D021381:Protein Transport / Q000187:drug effects; D017421:Sequence Analysis; D018724:Spinacia oleracea / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D009566:Nitrates; D010940:Plant Proteins
keywords: expression profiles; nitrate stress; pathogenesis-related protein; spinach