title: The utility of Caco-2 cells in isolation of enteroviruses from environmental and clinical material.
abstract: The work presented here demonstrates the utility of Caco-2 cells in the isolation of enteroviruses (EVs) from environmental and clinical materials. Thirty-two samples of cerebrospinal fluid positive in Pan-entero RT-PCR were taken for EV strain isolation in cell culture. Out of the 32 samples analysed, 22 (68.75%) were positive for enteroviruses by isolation in Caco-2 cells, and 10 (31.25%) were positive by isolation in RD cells. High viral titre in clinical specimens resulted in rate increase for isolation in Caco-2 cells and RD cells (87.5% and 50%, respectively). Also, the probability of isolation of enteroviruses from sewage in Caco-2 cells was 20 times higher that in RD cells. We proved that Caco-2 cells were more effective than RD cells in enterovirus isolation, irrespective of the material used in the inoculation process.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wieczorek
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ciaćka
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Witek
authors.forename: Bogumiła
authors.initials: B
authors.lastname: Litwińska
mesh_terms: D018938:Caco-2 Cells; D002555:Cerebrospinal Fluid / Q000821:virology; D004770:Enterovirus / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000134:cerebrospinal fluid / Q000821:virology; D006801:Humans; D008587:Meningitis, Viral / Q000175:diagnosis / Q000821:virology; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000821:virology*; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D012367:RNA, Viral; D012722:Sewage