title: MiR-9 promotes microglial activation by targeting MCPIP1.
abstract: Microglia participate in innate inflammatory responses within the central nervous system. The highly conserved microRNA-9 (miR-9) plays critical roles in neurogenesis as well as axonal extension. Its role in microglial inflammatory responses, however, remains poorly understood. Here we identify a unique role of miR-9 in mediating the microglial inflammatory response via distinct signalling pathways. MiR-9-mediated regulation of cellular activation involved downregulated expression of the target protein, monocyte chemotactic protein-induced protein 1 (MCPIP1) that is crucial for controlling inflammation. Results indicate that miR-9-mediated cellular activation involved signalling via the NF-κB pathway, but not the β-catenin pathway.
authors.affiliation: 1] Department of Pharmacology, Medical School of Southeast University, Nanjing, Jiangsu 210009, China [2].
authors.forename: Honghong
authors.initials: H
authors.lastname: Yao
authors.affiliation: 1] Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198-5880, USA [2].
authors.forename: Rong
authors.initials: R
authors.lastname: Ma
authors.affiliation: 1] Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198-5880, USA [2].
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198-5880, USA.
authors.forename: Guoku
authors.initials: G
authors.lastname: Hu
authors.affiliation: The first Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xufeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Duan
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198-5880, USA.
authors.forename: Yeonhee
authors.initials: Y
authors.lastname: Kook
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198-5880, USA.
authors.forename: Fang
authors.initials: F
authors.lastname: Niu
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198-5880, USA.
authors.forename: Ke
authors.initials: K
authors.lastname: Liao
authors.affiliation: Department of Basic Medical Science, School of Medicine, University of Missouri-Kansas City, Kansas City, Missouri 64108, USA.
authors.forename: Minggui
authors.initials: M
authors.lastname: Fu
authors.affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.affiliation: Burnett School of Biomedical Science, College of Medicine, University of Central Florida, Orlando, Florida 32816, USA.
authors.forename: Pappachan
authors.initials: P
authors.lastname: Kolattukudy
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198-5880, USA.
authors.forename: Shilpa
authors.initials: S
authors.lastname: Buch
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D005434:Flow Cytometry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D017628:Microglia / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: C551804:MIRN9 microRNA, mouse; C582333:MIRN9 microRNA, rat; D035683:MicroRNAs; D016328:NF-kappa B; D051176:beta Catenin; D012260:Ribonucleases; C541320:Zc3h12a protein, mouse