title: Expression of a codon-optimized Aspergillus niger pectin methylesterase gene in the methylotrophic yeast Candida boidinii.
abstract: A codon-optimized Aspergillus niger pectin methylesterase (PME) gene was expressed in the methylotrophic yeast Canidia boidinii. The PME-producing strains showed better growth on pectin than the wild-type strains, suggesting that the PME-producing strains could efficiently utilize methyl ester moieties of pectin. On the other hand, overproduction of PME negatively affected the proliferation of C. boidinii on leaves of Arabidopsis thaliana.
authors.affiliation: a Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake , Sakyo-ku, Kyoto , Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Kawaguchi
authors.forename: Hiroya
authors.initials: H
authors.lastname: Yurimoto
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Sakai
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D002175:Candida / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D003062:Codon / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D010368:Pectins / Q000378:metabolism
chemical_list: D003062:Codon; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase
keywords: heterologous expression; methanol; methylotrophic yeast; pectin methylesterase