title: Faecal carriage of extended-spectrum β-lactamase (ESBL)-producing enterobacteria in liver disease patients from two hospitals in Egypt and France: a comparative epidemiological study.
abstract: This study aimed to assess and compare the epidemiology of faecal carriage of extended spectrum β-lactamase-producing enterobacteria (ESBL-E) in Hepatology departments of two hospitals specializing in liver diseases, Theodor Bilharz Research Institute (TBRI) in Cairo (Egypt) and Beaujon Hospital (Bj) in Clichy (France). CTX-M groups were identified by PCR, and TEM and SHV derivatives with the check-point system. Phylogenetic groups of E. coli were determined by multiplex PCR, and clone ST131 by PCR of gene pabB. Prevalence of ESBL-E was 77·6% (45/58) in TBRI and 6·5% (13/199) in Bj (P < 10-7). Previous hospitalization was more common (P = 0·003) in Bj patients (93%) than in TBRI patients (45%) suggesting high prevalence of ESBL-E in the Egyptian community. The presence of E. coli B2 ST131 among ESBL-E faecal E. coli in Egypt confirms its pervasiveness in the community and raises concern regarding this highly virulent and resistant clone.
authors.affiliation: Microbiology Department,Theodor Bilharz Research Institute,Cairo,Egypt.
authors.forename: N S
authors.initials: NS
authors.lastname: Fam
authors.affiliation: Microbiology Department,Hôpital AP-HP Beaujon,Clichy,France.
authors.forename: S
authors.initials: S
authors.lastname: Defasque
authors.affiliation: Microbiology Department,Hôpital AP-HP Beaujon,Clichy,France.
authors.forename: F
authors.initials: F
authors.lastname: Bert
authors.affiliation: Microbiology Department,Hôpital AP-HP Beaujon,Clichy,France.
authors.forename: V
authors.initials: V
authors.lastname: Leflon-Guibout
authors.affiliation: Hepatology Department Theodor Bilharz Research Institute Cairo,Egypt.
authors.forename: A
authors.initials: A
authors.lastname: El-Ray
authors.affiliation: Hepatology Department Theodor Bilharz Research Institute Cairo,Egypt.
authors.forename: M
authors.initials: M
authors.lastname: El-Ghannam
authors.affiliation: Hepatology Department Theodor Bilharz Research Institute Cairo,Egypt.
authors.forename: M E
authors.initials: ME
authors.lastname: Attia
authors.affiliation: Microbiology Department,Theodor Bilharz Research Institute,Cairo,Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Omar
authors.affiliation: Microbiology Department,Theodor Bilharz Research Institute,Cairo,Egypt.
authors.forename: D G
authors.initials: DG
authors.lastname: Desouki
authors.affiliation: Hepatology Department,Hôpital AP-HP Beaujon,Clichy,France.
authors.forename: D
authors.initials: D
authors.lastname: Valla
authors.affiliation: Microbiology Department,Hôpital AP-HP Beaujon,Clichy,France.
authors.forename: M-H
authors.initials: MH
authors.lastname: Nicolas-Chanoine
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D004534:Egypt / Q000453:epidemiology; D004755:Enterobacteriaceae / Q000201:enzymology; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005602:France / Q000453:epidemiology; D006782:Hospitals, Special / Q000706:statistics & numerical data; D006801:Humans; D008107:Liver Diseases / Q000382:microbiology*; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D015995:Prevalence; D055815:Young Adult; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases
keywords: typing