title: Tebipenem pivoxyl. Derivative spectroscopy study of stability of the first oral carbapenem.
abstract: A simple and selective derivative spectrophotometric method was developed for the quantitative determination of tebipenem and its pivoxyl ester in the presence of degradation products formed during degradation in aqueous solutions (hydrolysis, oxidation, phosphate buffer pH ∼6.0) and in the solid state (photolysis, thermolysis in dry air and at an increased relative air humidity). The method was based on zero-crossing first-derivative spectrophotometry (λ=341 nm for tebipenem pivoxyl and λ=320 nm for tebipenem), which eliminated the overlapping caused by various degradation products. The selectivity of the method for determination of tebipenem pivoxyl and tepipenem during stability studies was an effect of lack of substituents containing π-bond system chromophores in degradation products. It was also confirmed by comparison of the experimental spectra sample with the theoretical UV spectra and their first derivatives which were obtained by using the density functional theory with the B3LYP hybrid functional and 6-31G(d,p) basis set. The method were linear in the concentration range 16.70-220.0 μg mL(-1) for tebipenem (λ=320 nm; r=0.9989) and 10.70-160.0 μg mL(-1) for tebipenem pivoxyl (λ=341 nm, r=0.9990). The limits of detection and quantitation were 4.72 and 15.60 μg mL(-1) for tebipenem and 2.54 and 8.40 μg mL(-1) for tebipenem pivoxyl, respectively. The method had a good intra-day precision (RSD from 0.12% to 0.62%) and inter-day precision (RSD from 0.22% to 2.13%). The recovery of tebipenem and tebipenem pivoxyl ranged from 99.61% to 99.86% and from 99.38% to 99.87%, respectively. First-derivative spectrophotometry was used for a routine analysis of tebipenem and its ester as well as to monitor the conversion of tebipenem pivoxyl to tebipenem and to predict their degradation pathways.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland. Electronic address: jpiontek@ump.edu.pl.
authors.forename: Judyta
authors.initials: J
authors.lastname: Cielecka-Piontek
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Paczkowska
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Zalewski
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Talaczyńska
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland.
authors.forename: Mikołaj
authors.initials: M
authors.lastname: Mizera
mesh_terms: D000284:Administration, Oral; D015780:Carbapenems / Q000737:chemistry*; D007700:Kinetics; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D015780:Carbapenems; C500135:tebipenem
keywords: Derivative spectrophotometry; Stability studies; Tebipenem; Tebipenem pivoxyl; Validation