title: Blood concentrations of D- and L-lactate in healthy rabbits.
abstract: OBJECTIVE
  To determine whole blood and serum concentrations of l-lactate and serum concentrations of d-lactate in healthy rabbits and compare three methods of analysis for l-lactate measurement.
  
  
  METHODS
  Prospective study using 25 rabbits. Concentrations of whole blood l-lactate were measured using a portable analyser and a blood gas analyser. The remainder of the sample was allowed to clot for centrifugation. Serum was stored at -20°C for determination of l- and d- lactate by high-performance liquid chromatography.
  
  
  RESULTS
  d-lactate values by high-performance liquid chromatography were 0 · 17 ± 0 · 08 mmol/L. l-lactate values were 5 · 1 (±2 · 1) mmol/L by high-performance liquid chromatography, 6 · 9 (±2 · 7) mmol/L with the portable analyser and 7 · 1 (±1 · 6) mmol/L with the blood gas analyser. No significant difference (P > 0 · 05) was found between the two analysers. Significant difference existed between serum l-lactate values obtained by high-performance liquid chromatography and the whole blood values obtained with the blood gas analyser (P < 0 · 01) and portable analyser (P < 0 · 05).
  
  
  CONCLUSIONS
  Serum concentrations of d-lactate in healthy rabbits are in the range of those of other mammals. l-lactate values in healthy rabbits are higher compared with other mammals. Good correlation was found between the portable and blood gas analysers for whole blood l-lactate measurement in healthy rabbits.
authors.affiliation: Department of Clinical Sciences, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, J2S 2M2, Canada.
authors.forename: I
authors.initials: I
authors.lastname: Langlois
authors.forename: A
authors.initials: A
authors.lastname: Planché
authors.forename: S R
authors.initials: SR
authors.lastname: Boysen
authors.forename: S
authors.initials: S
authors.lastname: Abeysekara
authors.forename: G A
authors.initials: GA
authors.lastname: Zello
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D005260:Female; D007773:Lactates / Q000097:blood*; D008297:Male; D011446:Prospective Studies; D011817:Rabbits / Q000097:blood*; D012016:Reference Values
chemical_list: D007773:Lactates