title: Genomic profiling of human Leishmania braziliensis lesions identifies transcriptional modules associated with cutaneous immunopathology.
abstract: The host immune response has a critical role not only in protection from human leishmaniasis but also in promoting disease severity. Although candidate gene approaches in mouse models of leishmaniasis have been extremely informative, a global understanding of the immune pathways active in lesions from human patients is lacking. To address this issue, genome-wide transcriptional profiling of Leishmania braziliensis-infected cutaneous lesions and normal skin controls was carried out. A signature of the L. braziliensis skin lesion was defined, which includes over 2,000 differentially regulated genes. Pathway-level analysis of this transcriptional response revealed key biological pathways present in cutaneous lesions, generating a testable 'metapathway' model of immunopathology and providing new insights for treatment of human leishmaniasis.
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Fernanda O
authors.initials: FO
authors.lastname: Novais
authors.affiliation: Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais-INCT-DT(CNPq/MCT), Serviço de Imunologia, Hospital Universitario Prof. Edgard Santos, Universidade Federal da Bahia Salvador, Bahia, Brazil.
authors.forename: Lucas P
authors.initials: LP
authors.lastname: Carvalho
authors.affiliation: Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais-INCT-DT(CNPq/MCT), Serviço de Imunologia, Hospital Universitario Prof. Edgard Santos, Universidade Federal da Bahia Salvador, Bahia, Brazil.
authors.forename: Sara
authors.initials: S
authors.lastname: Passos
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: David S
authors.initials: DS
authors.lastname: Roos
authors.affiliation: Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais-INCT-DT(CNPq/MCT), Serviço de Imunologia, Hospital Universitario Prof. Edgard Santos, Universidade Federal da Bahia Salvador, Bahia, Brazil.
authors.forename: Edgar M
authors.initials: EM
authors.lastname: Carvalho
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. Electronic address: pscott@vet.upenn.edu.
authors.forename: Phillip
authors.initials: P
authors.lastname: Scott
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. Electronic address: beiting@vet.upenn.edu.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Beiting
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D023281:Genomics / Q000379:methods*; D006801:Humans; D058847:Inflammasomes / Q000235:genetics / Q000276:immunology; D007892:Leishmania braziliensis / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000235:genetics / Q000276:immunology; D012867:Skin / Q000276:immunology / Q000469:parasitology; D012883:Skin Ulcer / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D014158:Transcription, Genetic / Q000276:immunology; D059467:Transcriptome / Q000276:immunology; D055815:Young Adult
chemical_list: D058847:Inflammasomes