title: High prevalence of pfcrt K76T and mdr1 N86Y mutations in Sonitpur district of Assam, India.
abstract: Present study reports the prevalence and distribution of pfcrt K76T and mdr1 N86Y mutations in malaria endemic areas of Sonitpur district of Assam. Out of 163 individuals tested for malaria, 67 (SPR = 41.1 %) were detected positive for malaria infection using rapid detection kit in the field and PCR assay in the laboratory. Nested PCR-RFLP assay was used to amplify pfcrt K76T and mdr1 N86Y genes flanking the K76T and N86Y mutations. P. falciparum was most abundant (91.04 %) among the three Plasmodium species reported and its prevalence was significantly higher as compared to P. vivax and P. malariae (χ(2) = 150.76; p ≤ 0.0001; df = 2). Malaria was equally distributed among all the age groups and both the sexes. Hemoglobin contents in severe anaemic patients had a significant linear decreasing trend among patients with the increase in age (χ(2) = 4.33; p = 0.03), whereas non severe anaemic patients exhibited significant linear increasing trend among the patients with the increase in age (χ(2) = 18.38; p ≤ 0.0001). Pfcrt K76T mutation was recorded in 44 (72.13 %) isolates, whereas mdr1 N86Y mutation could be detected in 28 (41.79 %) isolates only. Only 32.7 % of the samples had both pfcrt K76T and mdr N86Y mutations. Number of pfcrt K76T mutant isolates was significantly higher than the wild type. However no significant difference was observed among the number of isolates with mdr1 N86Y mutant and wild isolates.
authors.affiliation: Department of Medical Entomology, Defence Research Laboratory, Tezpur, Assam India.
authors.forename: Diganta
authors.initials: D
authors.lastname: Goswami
authors.affiliation: Department of Medical Entomology, Defence Research Laboratory, Tezpur, Assam India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Dhiman
authors.affiliation: Department of Medical Entomology, Defence Research Laboratory, Tezpur, Assam India.
authors.forename: Bipul
authors.initials: B
authors.lastname: Rabha
authors.affiliation: Department of Medical Entomology, Defence Research Laboratory, Tezpur, Assam India.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Department of Medical Entomology, Defence Research Laboratory, Tezpur, Assam India.
authors.forename: Indra
authors.initials: I
authors.lastname: Baruah
authors.affiliation: Department of Medical Entomology, Defence Research Laboratory, Tezpur, Assam India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Veer
authors.affiliation: Department of Zoology, Gauhati University, Guwahati, Assam India.
authors.forename: Rk
authors.initials: R
authors.lastname: Bhola
authors.affiliation: Department of Zoology, Gauhati University, Guwahati, Assam India.
authors.forename: Dk
authors.initials: D
authors.lastname: Sharma
keywords: Antimalarial resistance; Mutation; PCR assay; mdr1 N86Y; pfcrt K76T