title: Ethanol at low concentration attenuates diabetes induced lung injury in rats model.
abstract: To observe the changes of lung injury when diabetic rats were treated with low concentration of ethanol (EtOH) and analyze the related mechanisms, male Sprague-Dawley (SD) rats were divided into control, diabetic (DM), and EtOH+DM groups. Diabetic rat was mimicked by injection of streptozotocin intraperitoneally. Fasting blood glucose (FBG) level, lung weight (LW), body weight (BW), and LW/BW were measured. The changes of lung tissue and Type II alveolar cell were detected. Pulmonary malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were measured; meanwhile, ALDH2 mRNA and protein expressions were detected by RT-PCR and western blotting, respectively. Compared with control group, in DM group, SOD activity was decreased; FBG level, LW/BW, MDA content, ALDH2 mRNA, and protein expressions were decreased. Compared with DM group, in EtOH+DM group, SOD activity, ALDH2 mRNA, and protein expressions were increased; LW/BW and MDA content were decreased. The structures of lung tissue and lamellar bodies were collapsed in DM group; the injury was attenuated in EtOH+DM group. Our findings suggested that, in diabetic rat, pulmonary ALDH2 expression was decreased accompanying lung injury. EtOH at low concentration decreased diabetes induced lung injury through activating ALDH2 expression.
authors.affiliation: Department of Respiratory Disease, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China.
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Hu
authors.affiliation: Department of Physiology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu 233030, China.
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu 233030, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Disease, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China.
authors.forename: Pin-Fang
authors.initials: PF
authors.lastname: Kang
authors.affiliation: Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Disease, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China.
authors.forename: Hong-Ju
authors.initials: HJ
authors.lastname: Wang
authors.affiliation: Department of Physiology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu 233030, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Respiratory Disease, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Chen
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002492:Central Nervous System Depressants / Q000008:administration & dosage; D048909:Diabetes Complications / Q000188:drug therapy*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage*; D005786:Gene Expression Regulation; D008168:Lung / Q000378:metabolism; D055370:Lung Injury / Q000188:drug therapy*; D008297:Male; D008315:Malondialdehyde / Q000008:administration & dosage; D024101:Mitochondrial Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D002492:Central Nervous System Depressants; D024101:Mitochondrial Proteins; D000431:Ethanol; D008315:Malondialdehyde; D013311:Streptozocin; D013482:Superoxide Dismutase; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial; C505112:Aldh2 protein, rat