title: Effects of WT1 down-regulation on oocyte maturation and preimplantation embryo development in pigs.
abstract: The Wilms' tumour 1 (WT1) gene originally identified as a tumour suppressor associated with WTs encodes a zinc finger-containing transcription factor that is expressed in multiple tissues and is an important regulator of cellular and organ growth, proliferation, development, migration and survival. However, there is a deficiency of data regarding the expression and function of WT1 during oocyte maturation and preimplantation embryonic development. Herein, we sought to define the expression characteristics and functions of WT1 during oocyte maturation and preimplantation embryonic development in pigs. We show that WT1 is expressed in porcine oocytes and at all preimplantation stages in embryos generated by ICSI. We then evaluated the effects of down-regulating WT1 expression at germinal vesicle and early ICSI stages using a recombinant plasmid (pGLV3-WT1-shRNA). Down-regulation of WT1 did not affect oocyte maturation but significantly decreased preimplantation embryonic development and increased apoptosis in blastocysts. These results indicate that WT1 plays important roles in the development of porcine preimplantation embryos.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Guan
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: An
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China.
authors.forename: Anran
authors.initials: A
authors.lastname: Fan
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China.
authors.forename: Guangqi
authors.initials: G
authors.lastname: Song
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China.
authors.forename: Tianchuang
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China ziyi@jlu.edu.cn zhxueming@yahoo.com.
authors.forename: Xueming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo EngineeringCollege of Veterinary Medicine, Center for Animal Embryo Engineering of Jilin Province, Jilin University, 5333 Xi An Da Lu, Changchun, Jilin 130062, ChinaCollege of Medical Laboratory ScienceBeihua University, Jilin, Jilin 132013, ChinaInstitute of Special Animal and Plant ScienceChinese Academy of Agricultural Sciences, Changchun, Jilin 130122, China ziyi@jlu.edu.cn zhxueming@yahoo.com.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D001755:Blastocyst / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D018920:Coculture Techniques; D015536:Down-Regulation; D046149:Embryo Culture Techniques; D005260:Female; D018507:Gene Expression Regulation, Developmental; D059471:In Vitro Oocyte Maturation Techniques; D008297:Male; D009865:Oocytes / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D020554:Sperm Injections, Intracytoplasmic; D013552:Swine; D013997:Time Factors; D014162:Transfection; D025721:WT1 Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D025721:WT1 Proteins