title: Effect of 17β-estradiol on striatal dopaminergic transmission induced by permethrin in early childhood rats.
abstract: A positive effect of estrogen treatment has been observed in neurodegenerative diseases such as Parkinson's disease. Since 17β-estradiol can modulate positively dopaminergic system, here we sought to evaluate the effect of 17β-estradiol supplementation on an animal model developing dopaminergic alterations on nucleus of striatum after neonatal exposure to permethrin pesticide. The goal of the study was to verify if the co-treatment with 17β-estradiol could protect against the damage induced by pesticide exposure in early life. Permethrin treated rats showed a decrease of dopamine and Nurr1 gene expression in striatum, while a more pronounced decrease of dopamine was observed in rats co-administered with permethrin+17β-estradiol. No difference between control and permethrin treated rats was observed in both mRNA of ERα and ERβ, whereas the rats co-administered with permethrin+17β-estradiol showed a down-regulation of ERα expression. The in vitro studies showed that permethrin, at high concentration may have an antagonist effect on ERα and even more pronounced in ERβ, thus suggesting that permethrin may block the estrogen neuroprotective effects. In conclusion, in male rats, the administration of estrogen further enhanced the impairment of dopaminergic transmission due to exposure to permethrin.
authors.affiliation: School of Pharmacy, Pharmacology Unit, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, MC, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Nasuti
authors.affiliation: School of Pharmacy, Molecular Biology Unit, University of Camerino, Via Gentile III da Varano, 62032 Camerino, MC, Italy.
authors.forename: Manuel
authors.initials: M
authors.lastname: Carloni
authors.affiliation: School of Pharmacy, Molecular Biology Unit, University of Camerino, Via Gentile III da Varano, 62032 Camerino, MC, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Fedeli
authors.affiliation: Dipartimento di Scienze del Farmaco, Università G. d'Annunzio, Via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Di Stefano
authors.affiliation: Dipartimento di Scienze del Farmaco, Università G. d'Annunzio, Via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Lisa
authors.initials: L
authors.lastname: Marinelli
authors.affiliation: Dipartimento di Scienze del Farmaco, Università G. d'Annunzio, Via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Laura Serafina
authors.initials: LS
authors.lastname: Cerasa
authors.affiliation: Center of Excellence on Neurodegenerative Diseases University of Milan, Via Balzaretti, 9 20133 Milan, Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Meda
authors.affiliation: Center of Excellence on Neurodegenerative Diseases University of Milan, Via Balzaretti, 9 20133 Milan, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Maggi
authors.affiliation: School of Pharmacy, Molecular Biology Unit, University of Camerino, Via Gentile III da Varano, 62032 Camerino, MC, Italy. Electronic address: rosita.gabbianelli@unicam.it.
authors.forename: Rosita
authors.initials: R
authors.lastname: Gabbianelli
mesh_terms: D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000235:genetics; D047629:Estrogen Receptor beta / Q000235:genetics; D004967:Estrogens / Q000494:pharmacology*; D061986:MCF-7 Cells; D008297:Male; D017072:Neostriatum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2 / Q000235:genetics; D026023:Permethrin / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000187:drug effects*; D013997:Time Factors
chemical_list: D004785:Environmental Pollutants; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D018696:Neuroprotective Agents; C539884:Nr4a2 protein, rat; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D012333:RNA, Messenger; D004958:Estradiol; D026023:Permethrin; D004298:Dopamine
keywords: 17β-Estradiol; Dopaminergic neurons; Neurodegenerative disease; Permethrin; Rat