title: Proteomics elucidates key molecules involved in exsheathment in vitro in Oesophagostomum dentatum.
abstract: We explored molecules involved in in vitro exsheathment of Oesophagostomum dentatum L3s using a proteomic-transcriptomic-bioinformatic approach. Analysis of L3s before, during and after exsheathment identified 11 proteins that were over-expressed exclusively during exsheathment. These proteins (including key enzymes, heat shock, structural and nematode-specific proteins) were inferred to be involved in development, metabolism, structure, motility and/or host-parasite interactions. Some of these molecules represented homologues linked to entry into and exit from the dauer stage in the free-living nematode Caenorhabditis elegans. The approach established here provides a basis for investigations of ecdysis in other strongylid nematodes.
authors.affiliation: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Ondrovics
authors.affiliation: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria.
authors.forename: Katja
authors.initials: K
authors.lastname: Silbermayr
authors.affiliation: The Genome Institute, Washington University School of Medicine, MO 63108, USA; Division of Infectious Diseases, Department of Internal Medicine, Washington University School of Medicine, MO 63110, USA.
authors.forename: Makedonka
authors.initials: M
authors.lastname: Mitreva
authors.affiliation: Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Neil D
authors.initials: ND
authors.lastname: Young
authors.affiliation: Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: robinbg@unimelb.edu.au.
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
authors.affiliation: Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria. Electronic address: Anja.Joachim@vetmeduni.ac.at.
authors.forename: Anja
authors.initials: A
authors.lastname: Joachim
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology*; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D009815:Oesophagostomum / Q000235:genetics / Q000502:physiology*; D040901:Proteomics
chemical_list: D015801:Helminth Proteins
keywords: Exsheathment; Oesophagostomum dentatum; Parasitic nematode; Proteomics