title: Heterologous expression of Ceratophyllum demersum phytochelatin synthase, CdPCS1, in rice leads to lower arsenic accumulation in grain.
abstract: Recent studies have identified rice (Oryza sativa) as a major dietary source of inorganic arsenic (As) and poses a significant human health risk. The predominant model for plant detoxification of heavy metals is complexation of heavy metals with phytochelatins (PCs), synthesized non-translationally by PC synthase (PCS) and compartmentalized in vacuoles. In this study, in order to restrict As in the rice roots as a detoxification mechanism, a transgenic approach has been followed through expression of phytochelatin synthase, CdPCS1, from Ceratophyllum demersum, an aquatic As-accumulator plant. CdPCS1 expressing rice transgenic lines showed marked increase in PCS activity and enhanced synthesis of PCs in comparison to non-transgenic plant. Transgenic lines showed enhanced accumulation of As in root and shoot. This enhanced metal accumulation potential of transgenic lines was positively correlated to the content of PCs, which also increased several-fold higher in transgenic lines. However, all the transgenic lines accumulated significantly lower As in grain and husk in comparison to non-transgenic plant. The higher level of PCs in transgenic plants relative to non-transgenic presumably allowed sequestering and detoxification of higher amounts of As in roots and shoots, thereby restricting its accumulation in grain.
authors.affiliation: 1] Council of Scientific and Industrial Research - National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow-226001, INDIA [2] Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi-110 001, India.
authors.forename: Manju
authors.initials: M
authors.lastname: Shri
authors.affiliation: Council of Scientific and Industrial Research - National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow-226001, INDIA.
authors.forename: Richa
authors.initials: R
authors.lastname: Dave
authors.affiliation: Council of Scientific and Industrial Research - National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow-226001, INDIA.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Diwedi
authors.affiliation: Council of Scientific and Industrial Research - National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow-226001, INDIA.
authors.forename: Devesh
authors.initials: D
authors.lastname: Shukla
authors.affiliation: Council of Scientific and Industrial Research - National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow-226001, INDIA.
authors.forename: Ravi
authors.initials: R
authors.lastname: Kesari
authors.affiliation: 1] Council of Scientific and Industrial Research - National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow-226001, INDIA [2] Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi-110 001, India.
authors.forename: Rudra Deo
authors.initials: RD
authors.lastname: Tripathi
authors.affiliation: 1] Council of Scientific and Industrial Research - National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow-226001, INDIA [2] Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi-110 001, India.
authors.forename: Prabodh Kumar
authors.initials: PK
authors.lastname: Trivedi
authors.affiliation: 1] Council of Scientific and Industrial Research - National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow-226001, INDIA [2] Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi-110 001, India.
authors.forename: Debasis
authors.initials: D
authors.lastname: Chakrabarty
mesh_terms: D019881:Aminoacyltransferases / Q000096:biosynthesis* / Q000235:genetics; D001151:Arsenic / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D059022:Food Safety; D015870:Gene Expression; D005978:Glutathione / Q000378:metabolism; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
chemical_list: D002273:Carcinogens; D010940:Plant Proteins; D019881:Aminoacyltransferases; C093784:glutathione gamma-glutamylcysteinyltransferase; D005978:Glutathione; D001151:Arsenic