title: Anticancer activity of CopA3 dimer peptide in human gastric cancer cells.
abstract: CopA3 is a homodimeric α-helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigated the anticancer activity of CopA3 in human gastric cancer cells. CopA3 reduced cell viability and it was cytotoxic to gastric cancer cells in the MTS and LDH release assay, respectively. CopA3 was shown to induce necrotic cell death of the gastric cancer cells by flow cytometric analysis and acridine orange/ethidium bromide staining. CopA3-induced cell death was mediated by specific interactions with phosphatidylserine, a membrane component of cancer cells. Taken together, these data indicated that CopA3 mainly caused necrosis of gastric cancer cells, probably through interactions with phosphatidylserine, which suggests the potential utility of CopA3 as a cancer therapeutic.
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Korea.
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Korea.
authors.forename: In-Woo
authors.initials: IW
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Korea.
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Yun
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Korea.
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Nam
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Ahn
authors.affiliation: Ilsong Institute of Life Science, Hallym University, Anyang 431-060, Korea.
authors.forename: Dong-Chul
authors.initials: DC
authors.lastname: Kang
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Korea.
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D019476:Insect Proteins / Q000494:pharmacology*; D051379:Mice; D010718:Phosphatidylserines / Q000378:metabolism; D000067996:RAW 264.7 Cells; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; C583724:CopA3 peptide, Copris tripartitus; D019476:Insect Proteins; D010718:Phosphatidylserines