title: Genotyping of Giardia duodenalis isolates from dogs in Guangdong, China based on multi-locus sequence.
abstract: This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Guochao
authors.initials: G
authors.lastname: Zheng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Muhamd
authors.initials: M
authors.lastname: Alsarakibi
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Yuanjia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Tan
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D002681:China; D016000:Cluster Analysis; D060085:Coinfection / Q000469:parasitology / Q000662:veterinary; D003598:Cytoskeletal Proteins / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000469:parasitology / Q000662:veterinary*; D005969:Glutamate Dehydrogenase / Q000235:genetics; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D014305:Triose-Phosphate Isomerase / Q000235:genetics
chemical_list: D003598:Cytoskeletal Proteins; D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S; D005969:Glutamate Dehydrogenase; D014305:Triose-Phosphate Isomerase
keywords: China; Giardia duodenalis; Guangdong; dog; multi-locus sequence typing