title: Development and characterization of mouse monoclonal antibodies reactive with chicken IL-1β.
abstract: Interleukin-1β proteins from chicken, duck, goose, turkey, and pigeon share 77 to 99% amino acid sequence similarity among themselves, and only 31 to 35% sequence similarity is shared between avian and mammalian IL-1β. There have been no antibodies that specifically detect avian IL-1β, and the current study was conducted to develop mouse monoclonal antibodies (mAb) against chicken IL-1β (chIL-1β) to further define its biochemical and immunological properties. In this study, 2 mouse mAb that are specific for chIL-1β were produced and characterized. Both mAb identified a 66.0 kDa recombinant chIL-1β protein expressed in Escherichia coli by Western blot analysis that corresponded to the expected molecular weight of a recombinant fusion protein containing the full-length 23.0 kDa chIL-1β protein and a 43.0 kDa maltose binding protein tag. Immunohistochemical analysis identified cells producing endogenous chIL-1β in the cecal tonsils, bursa of Fabricius, and spleen. Purified recombinant chIL-1β dose-dependently stimulated the proliferation and nitric oxide production by thymocytes, and both activities were inhibited by co-incubation with the 2 chIL-1β mAb described in this paper. These mAb will be important immune reagents for basic and applied poultry research of IL-1β in poultry.
authors.affiliation: National Academy of Agricultural Science, Rural Development Administration, Suwon, Gyeonggi, 441-853, Korea Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705.
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 Hyun.Lillehoj@ars.usda.gov.
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705.
authors.forename: M S
authors.initials: MS
authors.lastname: Jeong
authors.affiliation: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza 500015, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Del Cacho
authors.affiliation: National Academy of Agricultural Science, Rural Development Administration, Suwon, Gyeonggi, 441-853, Korea.
authors.forename: J B
authors.initials: JB
authors.lastname: Kim
authors.affiliation: National Academy of Agricultural Science, Rural Development Administration, Suwon, Gyeonggi, 441-853, Korea.
authors.forename: H R
authors.initials: HR
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine and Research Institute of Life Science, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea.
authors.forename: W
authors.initials: W
authors.lastname: Min
authors.affiliation: Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang City, Kyunggido, 430-824, Korea.
authors.forename: H Y
authors.initials: HY
authors.lastname: Jeoung
authors.affiliation: Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang City, Kyunggido, 430-824, Korea.
authors.forename: D J
authors.initials: DJ
authors.lastname: An
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D030161:Avian Proteins / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology*; D008221:Lymphoid Tissue; D051379:Mice / Q000276:immunology; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D030161:Avian Proteins; D053583:Interleukin-1beta; D011993:Recombinant Fusion Proteins
keywords: chicken; interleukin 1β; monoclonal antibody; proliferation; thymus