title: Surprising prenatal toxicity of epidermal lipoxygenase-3.
abstract: Metabolites of the epidermal lipoxygenase-3 (eLOX-3) are involved in various metabolic pathways. Most unexpectedly, intra-amniotic delivery of eLOX-3 to mice at gestational day 14.5, both via an adenoviral vector and as recombinant protein, resulted in fetal growth restriction and intrauterine death. Periodic acid-Schiff staining and RT-PCR analysis of placentae from fetuses exposed to eLOX-3 indicated a lack of glycogen trophoblasts in the junctional zone. Placenta-specific gene expression was altered. Thus, the observed prenatal toxicity of eLOX-3 could be due to a strong effect on placental development.
authors.affiliation: Department of Pediatrics, University of Erlangen-Nürnberg, Children's Hospital, 91054 Erlangen, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Vierling
authors.affiliation: Department of Pediatrics, University of Erlangen-Nürnberg, Children's Hospital, 91054 Erlangen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Dick
authors.affiliation: Department of Pediatrics, University of Erlangen-Nürnberg, Children's Hospital, 91054 Erlangen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Wahlbuhl
authors.affiliation: German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Krieg
authors.affiliation: Department of Obstetrics and Gynaecology, Laboratory for Molecular Medicine, University Hospital Erlangen, 91054 Erlangen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Henke
authors.affiliation: Department of Obstetrics and Gynaecology, Laboratory for Molecular Medicine, University Hospital Erlangen, 91054 Erlangen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Rübner
authors.affiliation: Department of Pediatrics, University of Erlangen-Nürnberg, Children's Hospital, 91054 Erlangen, Germany. Electronic address: holm.schneider@uk-erlangen.de.
authors.forename: H
authors.initials: H
authors.lastname: Schneider
mesh_terms: D000256:Adenoviridae; D000818:Animals; D005260:Female; D005315:Fetal Diseases / Q000628:therapy; D046128:Fetal Therapies; D015316:Genetic Therapy; D007057:Ichthyosis / Q000628:therapy; D008084:Lipoxygenase / Q000008:administration & dosage / Q000009:adverse effects* / Q000235:genetics; D051379:Mice; D011247:Pregnancy; D017211:Treatment Failure
chemical_list: D008084:Lipoxygenase; C518403:eLOX3 protein, mouse
keywords: Epidermal lipoxygenase; Fetal growth restriction; Glycogen trophoblast; Intrauterine death; Prenatal toxicity