title: [Berberine inhibited apoptosis of human umbilical vein endothelial cells induced by Staphylocoocus aureus: an experimental research].
abstract: OBJECTIVE
  To study the inhibition of berberine (BBR) against ECV-304 apoptosis induced by Staphylococcus aureus (S. aureus).
  
  
  METHODS
  ECV-304 cells were pre-treated with 128 microg/mL BBR for 2 h and then S. aureus was added (1:100). The viability of cells was detected by MTT (3-4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The morphological changes were observed by Hoechst 33258 staining. The protection of BBR for infected cells was detected by DNA Ladder.
  
  
  RESULTS
  ECV-304 cells' viability were not obviously affected by berberine. But S. aureus induced ECV-304 cells' viability could be significantly inhibited by pre-treatment of BBR (P < 0.05). Besides S. aureus-induced ECV-304 apoptosis could be reduced, with significantly lessened apoptotic body and unobvious DNA degradation.
  
  
  CONCLUSIONS
  BBR could significantly inhibit S. aureus induced ECV-304 apoptosis.
authors.forename: Chuan-yin
authors.initials: CY
authors.lastname: Xiong
authors.forename: Yong-hang
authors.initials: YH
authors.lastname: Fu
authors.forename: Han-bin
authors.initials: HB
authors.lastname: Hu
authors.forename: Ai-fen
authors.initials: AF
authors.lastname: Bi
authors.forename: De-cui
authors.initials: DC
authors.lastname: Pei
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001599:Berberine / Q000494:pharmacology*; D002460:Cell Line; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D013211:Staphylococcus aureus
chemical_list: D001599:Berberine