title: [Permanent proteins in healthy human's urine in the experiment with 520-day isolation].
abstract: Purpose of the study was to track permanent proteins of urine proteome in the 520-day isolation experiment at the IBMP Ground-Based Test Facility with controlled environmental parameters. Object of the investigation was urine sampled from 6 normal male subjects at the age of 25 to 37 years. Second morning aliquots were gathered during baseline data collection, on days 50, 93, 124, 153, 180, 251, 274, 303, 330, 371, 400 and 427 of isolation, and in 7 days after its completion. Samples were subject to chromatography-mass spectrometry; results were analyzed with the help of bioinformatics resources. The following 7 permanent proteins were observed in urine over the entire length of the investigation: epidermal growth factor, polymer immunoglobulin receptor, plasma serine protease inhibitor, protein AMBP, keratin, type II cytoskeletal 1, collagen alpha-1 (vi) chain, serum albumin.
authors.forename: L Kh
authors.initials: LKh
authors.lastname: Pastushkova
authors.forename: K S
authors.initials: KS
authors.lastname: Kireev
authors.forename: A S
authors.initials: AS
authors.lastname: Kononikhin
authors.forename: E S
authors.initials: ES
authors.lastname: Tiys
authors.forename: I A
authors.initials: IA
authors.lastname: Popov
authors.forename: I V
authors.initials: IV
authors.lastname: Dobrokhotov
authors.forename: M A
authors.initials: MA
authors.lastname: Custaud
authors.forename: V A
authors.initials: VA
authors.lastname: Ivanisenko
authors.forename: N A
authors.initials: NA
authors.lastname: Kolchanov
authors.forename: E N
authors.initials: EN
authors.lastname: Nikolaev
authors.forename: V I
authors.initials: VI
authors.lastname: Pochuev
authors.forename: I M
authors.initials: IM
authors.lastname: Larina
mesh_terms: D000328:Adult; D002845:Chromatography; D019295:Computational Biology; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D011506:Proteins / Q000378:metabolism*; D011507:Proteinuria / Q000209:etiology*; D020543:Proteome; D012934:Social Isolation; D013026:Space Flight; D018561:Space Simulation; D013997:Time Factors; D014556:Urine / Q000737:chemistry
chemical_list: D011506:Proteins; D020543:Proteome