title: Guiding the behaviors of human umbilical vein endothelial cells with patterned silk fibroin films.
abstract: Silk fibroin is an ideal blood vessel substitute due to its advantageous qualities including variable size, good suture retention, low thrombogenicity, non-toxicity, non-immunogenicity, biocompatibility, and controllable biodegradation. In this study, silk fibroin films with a variety of surface patterns (e.g. square wells, round wells plus square pillars, square pillars, and gratings) were prepared for in vitro characterization of human umbilical vein endothelial cell's (HUVEC) response. The affects of biomimetic length-scale topographic cues on the cell orientation/elongation, proliferation, and cell-substrate interactions have been investigated. The density of cells is significantly decreased in response to the grating patterns (70±3nm depth, 600±8nm pitch) and the square pillars (333±42nm gap). Most notably, we observed the contact guidance response of filopodia of cells cultured on the surface of round wells plus square pillars. Overall, our data demonstrates that the patterned silk fibroin films have an impact on the behaviors of human umbilical vein endothelial cells.
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou 215006, China; The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Du
authors.affiliation: The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China. Electronic address: yuanl@suda.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou 215006, China; The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Weng
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou 215006, China; The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China. Electronic address: gchen@suda.edu.cn.
authors.forename: Gaojian
authors.initials: G
authors.lastname: Chen
authors.affiliation: Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou 215006, China; The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China. Electronic address: zhijun.hu@suda.edu.cn.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Hu
mesh_terms: D049109:Cell Proliferation; D005349:Fibroins / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D047011:Silk / Q000737:chemistry*
chemical_list: D047011:Silk; D005349:Fibroins
keywords: Cell morphology; Cell proliferation; Endothelial cell; Silk fibroin film; Surface pattern