title: Treating cell culture media with UV irradiation against adventitious agents: minimal impact on CHO performance.
abstract: Sterility of cell culture media is an important concern in biotherapeutic processing. In large scale biotherapeutic production, a unit contamination of cell culture media can have costly effects. Ultraviolet (UV) irradiation is a sterilization method effective against bacteria and viruses while being non-thermal and non-adulterating in its mechanism of action. This makes UV irradiation attractive for use in sterilization of cell culture media. The objective of this study was to evaluate the effect of UV irradiation of cell culture media in terms of chemical composition and the ability to grow cell cultures in the treated media. The results showed that UV irradiation of commercial cell culture media at relevant disinfection doses impacted the chemical composition of the media with respect to several carboxylic acids, and to a minimal extent, amino acids. The cumulative effect of these changes, however, did not negatively influence the ability to culture Chinese Hamster Ovary cells, as evaluated by cell viability, growth rate, and protein titer measurements in simple batch growth compared with the same cells cultured in control media exposed to visible light.
authors.affiliation: Waterloo Inst. for Nanotechnology, Dept. of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada, N2L3G1.
authors.forename: Sandi
authors.initials: S
authors.lastname: Yen
authors.forename: Stanislav
authors.initials: S
authors.lastname: Sokolenko
authors.forename: Bhavik
authors.initials: B
authors.lastname: Manocha
authors.forename: Eric J M
authors.initials: EJ
authors.lastname: Blondeel
authors.forename: Marc G
authors.initials: MG
authors.lastname: Aucoin
authors.forename: Ankit
authors.initials: A
authors.lastname: Patras
authors.forename: Farnaz
authors.initials: F
authors.lastname: Daynouri-Pancino
authors.forename: Michael
authors.initials: M
authors.lastname: Sasges
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000528:radiation effects*; D004203:Disinfection / Q000379:methods*; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins
keywords: CHO; adventitious agents; cell culture; irradiation; ultraviolet