title: Prognostic value of coexistence of abnormal expression of micro-RNA-200b and cyclic adenosine monophosphate-responsive element-binding protein 1 in human astrocytoma.
abstract: Our aim was to investigate the expression of micro-RNA-200b (miR-200b) and cAMP-responsive element-binding protein 1 (CREB-1) in astrocytoma and its efficacy for predicting outcome. Both miR-200b and CREB-1 messenger RNA expression was measured in 122 astrocytomas and 30 nonneoplastic brain specimens by quantitative real-time polymerase chain reaction. Expression of miR-200b was significantly lower in astrocytoma than in nonneoplastic brain (P < .001), whereas CREB-1 messenger RNA expression was significantly elevated in the tumors (P < .001). Both miR-200b down-regulation and CREB-1 up-regulation were significantly associated with advanced pathologic grade (P = .002 and P = .006, respectively). Low miR-200b expression correlated negatively with Karnofsky performance score (P = .03), and high CREB-1 expression correlated positively with mean tumor diameter (P = .03). By Kaplan-Meier analysis, low miR-200b, high CREB-1, and coexistence of abnormal miR-200b and CREB-1 expression (low miR-200b/high CREB-1) were predictive of shorter progression-free survival and overall survival in both grade III and grade IV astrocytoma. By multivariate analysis, only low miR-200b/high CREB-1 expression was an independent prognostic factor for poor prognosis in astrocytoma of advanced grade. Both miR-200b and CREB-1 may play important cooperative roles in the progression of human astrocytoma. The efficacy of miR-200b and CREB-1 together as a predictor of prognosis in astrocytoma patients is shown for the first time.
authors.affiliation: Department of Oncology, the 264th Hospital of PLA, Taiyuan, 030001, China.
authors.forename: Jun-qing
authors.initials: JQ
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Baoji Central Hospital, Baoji, 721008, China.
authors.forename: Qing-he
authors.initials: QH
authors.lastname: Yao
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: Yong-qin
authors.initials: YQ
authors.lastname: Kuang
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: Li-bin
authors.initials: LB
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: Hai-dong
authors.initials: HD
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: Jing-ming
authors.initials: JM
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: En-yu
authors.initials: EY
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: Ke-xia
authors.initials: KX
authors.lastname: Fan
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Xia
authors.affiliation: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan Province, 610083, China. Electronic address: gujwcd@126.com.
authors.forename: Jian-wen
authors.initials: JW
authors.lastname: Gu
mesh_terms: D000368:Aged; D001254:Astrocytoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis; D001932:Brain Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000032:analysis / Q000096:biosynthesis*; D018450:Disease Progression; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000096:biosynthesis*; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; C533896:MIRN200 microRNA, human; D035683:MicroRNAs
keywords: Astrocytoma; CREB-1; Micro-RNA-200b; Prognosis; Tumor progression