title: In vitro diagnosis of immediate IgE-mediated drug hypersensitivity: warnings and (unmet) needs.
abstract: Immediate drug hypersensitivity reactions (DHR) constitute an important health condition, with serious consequences of inadequate diagnosis. In this article, some of the most important issues related to in vitro diagnosis of IgE-mediated allergies are discussed. In vitro diagnostics will benefit from expanded and novel insights and understandings in drug chemical reactivity, protein binding, biotransformation, degradation, identification of (cross-reactive) drug antigenic determinants, and deeper understanding of sensitization routes. Collective efforts should be undertaken to activate fundamental and clinical investigations.
authors.affiliation: Department of Immunology, Allergology, Rheumatology, Faculty of Medicine and Health Science, Antwerp University Hospital, University of Antwerp, Antwerp 2610, Belgium.
authors.forename: Astrid P
authors.initials: AP
authors.lastname: Uyttebroek
authors.affiliation: Department of Immunology, Allergology, Rheumatology, Faculty of Medicine and Health Science, Antwerp University Hospital, University of Antwerp, Antwerp 2610, Belgium.
authors.forename: Vito
authors.initials: V
authors.lastname: Sabato
authors.affiliation: Department of Immunology, Allergology, Rheumatology, Faculty of Medicine and Health Science, Antwerp University Hospital, University of Antwerp, Antwerp 2610, Belgium.
authors.forename: Chris H
authors.initials: CH
authors.lastname: Bridts
authors.affiliation: Department of Immunology, Allergology, Rheumatology, Faculty of Medicine and Health Science, Antwerp University Hospital, University of Antwerp, Antwerp 2610, Belgium. Electronic address: immuno@uantwerpen.be.
authors.forename: Didier G
authors.initials: DG
authors.lastname: Ebo
mesh_terms: D004342:Drug Hypersensitivity / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology
chemical_list: D007073:Immunoglobulin E
keywords: Basophil activation; Drug provocation; Flow cytometry; IgE; Immediate drug allergy