title: Decorin accelerates the liver regeneration after partial hepatectomy in fibrotic mice.
abstract: BACKGROUND
  Considering the existence of a large number of liver cell degeneration and necrosis in fibrotic liver, liver function was damaged severely and could not effectively regenerate after partial hepatectomy (PHx). The aim of this study was to investigate whether decorin (DCN) could promote the liver regeneration after PHx in fibrotic mice.
  
  
  METHODS
  Forty mice (5-week-old, Balb/c) were injected with CCl4 intraperitoneally and liver fibrosis model was established after 5 weeks. The survival mice were randomly divided into two groups: control group and DCN group. Then, we performed 70% PHx on all these mice and injected DCN or phosphate-buffered saline plus normal saline (NS) to each group, respectively, after surgery. Liver body weight ratio (LBR), quantitative real-time polymerase chain reaction, and immunohistochemistry were used to analyze liver regeneration and fibrosis degree in both groups, and to find out whether exogenous protein DCN could promote the regeneration of fibrosis liver after PHx.
  
  
  RESULTS
  Expressions of a-smooth muscle actin (SMA) mRNA and LBR had significant increases in the DCN group at postoperative Day 3 (POD 3, P < 0.05). The protein expressions of CD31, a-SMA, and tumor necrosis factor (TNF)-a were higher in the DCN group than those in the control group by immunohistochemistry at POD 3 (P < 0.05).
  
  
  CONCLUSIONS
  Exogenous protein DCN could promote liver regeneration after PHx in fibrotic mice.
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital, College of Medicine; the Institute of Minimally Invasive Surgery, Zhejiang University, Hangzhou, Zhejiang 310016, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital, College of Medicine; the Institute of Minimally Invasive Surgery, Zhejiang University, Hangzhou, Zhejiang 310016, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital, College of Medicine; the Institute of Minimally Invasive Surgery, Zhejiang University, Hangzhou, Zhejiang 310016, China.
authors.forename: Zheyong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital, College of Medicine; the Institute of Minimally Invasive Surgery, Zhejiang University, Hangzhou, Zhejiang 310016, China.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital, College of Medicine; the Institute of Minimally Invasive Surgery, Zhejiang University, Hangzhou, Zhejiang 310016, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital, College of Medicine; the Institute of Minimally Invasive Surgery, Zhejiang University, Hangzhou, Zhejiang 310016, China. Email: cxjzu@hotmail.com.
authors.forename: Xiujun
authors.initials: X
authors.lastname: Cai
mesh_terms: D000818:Animals; D058575:Decorin / Q000627:therapeutic use*; D006498:Hepatectomy; D007150:Immunohistochemistry; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000378:metabolism / Q000601:surgery*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D058575:Decorin; D019408:Platelet Endothelial Cell Adhesion Molecule-1