title: Mycobacterium marinum infection in Japanese forest green tree frogs (Rhacophorus arboreus).
abstract: Four Japanese forest green tree frogs (Rhacophorus arboreus) were presented with emaciation, abdominal distention and ulcerative and nodular cutaneous lesions affecting the brisket, limbs, digits and ventral abdomen. Another three frogs had been found dead in the same tank 1 year previously. Necropsy examination of these seven frogs revealed splenomegaly and hepatomegaly, with multiple tan-yellow nodular foci present in the liver, spleen, heart, lungs, ovaries and kidneys. Microscopically, five frogs had necrosis and surrounding granulomatous inflammation in the liver, spleen, kidneys, lungs, intestine and ovaries, with numerous acid-fast bacilli in the areas of necrosis. Two frogs had granulomatous lesions in the lungs, liver, spleen, heart, coelomic membrane, stomach and intestinal wall. These lesions had no or minimal necrosis and few acid-fast bacilli. Mycobacterium spp. was cultured from three frogs and identified as Mycobacterium marinum by colony growth rate and photochromogenicity and DNA sequencing. This is the first report of M. marinum infection in Japanese forest green tree frogs.
authors.affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Haridy
authors.affiliation: Gifu World Fresh Water Aquarium, Gifu, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tachikawa
authors.affiliation: National Hospital Organization, Kinki-Chuo Chest Medical Centre, Sakai, Osaka 591-8555, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: National Hospital Organization, Kinki-Chuo Chest Medical Centre, Sakai, Osaka 591-8555, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tsuyuguchi
authors.affiliation: National Hospital Organization, Kinki-Chuo Chest Medical Centre, Sakai, Osaka 591-8555, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tomita
authors.affiliation: The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose-shi, Tokyo 204-8533, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Maeda
authors.affiliation: Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Wada
authors.affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ibi
authors.affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.affiliation: Department of Pathogenetic Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. Electronic address: yanai@gifu-u.ac.jp.
authors.forename: T
authors.initials: T
authors.lastname: Yanai
mesh_terms: D000818:Animals; D065928:Forests; D007564:Japan; D009165:Mycobacterium Infections, Nontuberculous / Q000473:pathology / Q000662:veterinary*; D063188:Rana clamitans
keywords: Japanese forest green tree frog; Mycobacterium marinum; Rhacophorus arboreus; mycobacteriosis