title: Enhancement of (R)-1,3-butanediol production by engineered Escherichia coli using a bioreactor system with strict regulation of overall oxygen transfer coefficient and pH.
abstract: (R)-1,3-butanediol ((R)-1,3-BD) is an important substrate for the synthesis of industrial chemicals. Despite its large demand, a bioprocess for the efficient production of 1,3-BD from renewable resources has not been developed. We previously reported the construction of recombinant Escherichia coli that could efficiently produce (R)-1,3-BD from glucose. In this study, the fermentation conditions were optimized to further improve 1,3-BD production by the recombinant strain. A batch fermentation was performed with an optimized overall oxygen transfer coefficient (82.3 h(-1)) and pH (5.5); the 1,3-BD concentration reached 98.5 mM after 36 h with high-yield (0.444 mol (mol glucose)(-1)) and a high maximum production rate (3.63 mM h(-1)). In addition, a fed-batch fermentation enabled the recombinant strain to produce 174.8 mM 1,3-BD after 96 h cultivation with a yield of 0.372 mol (mol glucose)(-1), a maximum production rate of 3.90 mM h(-1), and a 98.6% enantiomeric excess (% ee) of (R)-1,3-BD.
authors.affiliation: a Japan Society for the Promotion of Science , Hiroshima , Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Kataoka
authors.forename: Alisa S
authors.initials: AS
authors.lastname: Vangnai
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Ueda
authors.forename: Takahisa
authors.initials: T
authors.lastname: Tajima
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakashimada
authors.forename: Junichi
authors.initials: J
authors.lastname: Kato
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology*; D002072:Butylene Glycols / Q000378:metabolism*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D060847:Metabolic Engineering; D010100:Oxygen / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; C028491:1,3-butylene glycol; D010100:Oxygen
keywords: 1,3-butanediol; bioreactor; engineered E. coli; metabolic engineering; synthetic biology