title: Cancer metabolism: cross talk between signaling and O-GlcNAcylation.
abstract: Cancer cells exhibit a unique metabolic shift to aerobic glycolysis that has been exploited diagnostically and therapeutically in the clinic. Oncogenes and tumor suppressors alter signaling pathways that lead to alterations of glycolytic flux. Stemming from glycolysis, the hexosamine biosynthetic pathway leads to elevated posttranslational addition of O-linked-β-N-acetylglucosamine (O-GlcNAc) on a diverse population of nuclear and cytosolic proteins, many of which regulate signaling pathways. This unit outlines techniques used to detect metabolic alterations in cancer cells, regulation by signaling pathways, and cellular O-GlcNAcylation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15th Street, Philadelphia, PA, 19102, USA.
authors.forename: Christina M
authors.initials: CM
authors.lastname: Ferrer
authors.forename: Mauricio J
authors.initials: MJ
authors.lastname: Reginato
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D053898:Biosynthetic Pathways; D045744:Cell Line, Tumor; D004734:Energy Metabolism; D006019:Glycolysis; D006031:Glycosylation; D006595:Hexosamines / Q000378:metabolism; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: D006595:Hexosamines; D015513:Oncogene Proteins; D025521:Tumor Suppressor Proteins; D000117:Acetylglucosamine