title: Do the same traffic rules apply? Directional chromosome segregation by SpoIIIE and FtsK.
abstract: Over a decade of studies have tackled the question of how FtsK/SpoIIIE translocases establish and maintain directional DNA translocation during chromosome segregation in bacteria. FtsK/SpoIIIE translocases move DNA in a highly processive, directional manner, where directionality is facilitated by sequences on the substrate DNA molecules that are being transported. In recent years, structural, biochemical, single-molecule and high-resolution microscopic studies have provided new insight into the mechanistic details of directional DNA segregation. Out of this body of work, a series of models have emerged and, ultimately, yielded two seemingly opposing models: the loading model and the target search model. We review these recent mechanistic insights into directional DNA movement and discuss the data that may serve to unite these suggested models, as well as propose future directions that may ultimately solve the debate.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA, 02138, USA.
authors.forename: Marina
authors.initials: M
authors.lastname: Besprozvannaya
authors.forename: Briana M
authors.initials: BM
authors.lastname: Burton
mesh_terms: D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002455:Cell Division; D020090:Chromosome Segregation; D008954:Models, Biological
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; C030069:spore-specific proteins, Bacillus