title: Infrared LED light therapy influences the expression of fibronectin and tenascin in skin wounds of malnourished rats--a preliminary study.
abstract: The aim of this investigation was to evaluate the effect of infrared (λ 846±20nm) LED irradiation on the expression profile of the extracellular matrix protein components, tenascin and fibronectin on skin wounds induced in well nourished and malnourished rats. Eighteen albino rats (21 days old) were randomly divided into a well-nourished group (standard diet) and a malnourished group (regional basic diet). After receiving the diet for 70 days, skin wounds were created and the animals were subdivided into three groups: well-nourished control (n=6), malnourished control (n=6), and malnourished+LED irradiated (λ 846±20nm, 100mW, 4J/cm(2)) (n=6). The animals were sacrificed 3 and 7 days after injury and histological sections were immunostained for both proteins. They were examined for the presence, intensity, distribution and pattern of immunolabeling. At 3 days, the distribution of tenascin was shown to be greater in the wound bed of malnourished animals compared to the well-nourished group. The intensity and distribution of tenascin was shown to be lower in the malnourished LED irradiated group compared to the malnourished control. There was a significant difference regarding the presence of fibronectin in the malnourished and well-nourished groups after 7 days (p=0.03). The intensity of fibronectin was slight (100%) in the irradiated group and moderate to intense in the malnourished control group. The results of the present study indicate that infrared LED irradiation modulates positively the expression of tenascin and particularly fibronectin.
authors.affiliation: Laboratory of Surgical Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Ana Paula Cavalcanti
authors.initials: AP
authors.lastname: de Sousa
authors.affiliation: Laboratory of Surgical Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Clarissa Araújo
authors.initials: CA
authors.lastname: Gurgel
authors.affiliation: Oswaldo Cruz Foundation, Laboratory of Pathology and Molecular Biology, Salvador, Bahia, Brazil.
authors.forename: Eduardo Antônio Gonçalves
authors.initials: EA
authors.lastname: Ramos
authors.affiliation: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Renan Ferreira
authors.initials: RF
authors.lastname: Trindade
authors.affiliation: Oswaldo Cruz Foundation, Laboratory of Pathology and Molecular Biology, Salvador, Bahia, Brazil.
authors.forename: Ludmila
authors.initials: L
authors.lastname: de Faro Valverde
authors.affiliation: Food Biochemistry Laboratory, School of Nutrition, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Tuânia Soares
authors.initials: TS
authors.lastname: Carneiro
authors.affiliation: Oral Epidemiology and Pediatric Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Maria Cristina Teixeira
authors.initials: MC
authors.lastname: Cangussú
authors.affiliation: Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
authors.forename: Antonio Luiz Barbosa
authors.initials: AL
authors.lastname: Pinheiro
authors.affiliation: Laboratory of Surgical Pathology, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil. Electronic address: jeanpatol@gmail.com.
authors.forename: Jean Nunes
authors.initials: JN
authors.lastname: Dos Santos
mesh_terms: D000818:Animals; D005353:Fibronectins / Q000378:metabolism*; D015870:Gene Expression / Q000528:radiation effects; D007259:Infrared Rays; D008297:Male; D044342:Malnutrition / Q000503:physiopathology*; D017208:Rats, Wistar; D012867:Skin / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects; D019063:Tenascin / Q000378:metabolism*; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D005353:Fibronectins; D019063:Tenascin
keywords: Fibronectin; LED phototherapy; Light therapy; Malnourishment; Tenascin; Undernourishment; Wound healing