title: Bioassay-guided isolation and identification of anti-platelet-active compounds from the root of Ashitaba (Angelica keiskei Koidz.).
abstract: Platelet aggregation is fundamental to a wide range of physiological and pathological processes, including the induction of thrombosis and arteriosclerosis. Anti-platelet activity of a crude methanol extract and solvent fractions of Ashitaba roots (Angelica keiskei Koidz.) was evaluated using a turbidimetric method using washed rabbit platelets. We identified the anti-platelet activities of two chalcones, 4-hydroxyderricin and xanthoangelol, isolated from the ethyl acetate-soluble fraction of Ashitaba roots by using a bioassay-guided isolation method. 4-Hydroxyderricin and xanthoangelol effectively inhibited platelet aggregation induced by collagen (IC50 of 41.9 and 35.9 μM, respectively), platelet-activating factor (IC50 of 46.1 and 42.3 μM, respectively) and phorbol 12-myristate 13-acetate (IC50 of 16.5 and 45.9 μM, respectively). These compounds did not inhibit thrombin-induced platelet aggregation (IC50 of>80 μM). The results suggest that the chalcones 4-hydroxyderricin and xanthoangelol may be potent anti-thrombotic components of A. keiskei Koidz.
authors.affiliation: a School of Applied Bioscience, Kyungpook National University , Daegu 702-701 , South Korea.
authors.forename: Dong Ju
authors.initials: DJ
authors.lastname: Son
authors.forename: Ye Oak
authors.initials: YO
authors.lastname: Park
authors.forename: Chengguang
authors.initials: C
authors.lastname: Yu
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Park
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D000933:Antifibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001681:Biological Assay; D002599:Chalcone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D018517:Plant Roots / Q000737:chemistry; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D000933:Antifibrinolytic Agents; D010975:Platelet Aggregation Inhibitors; C068243:4-hydroxyderricin; D002599:Chalcone; C068244:xanthoangelol
keywords: 4-hydroxyderricin; Angelica keiskei Koidaz; Ashitaba; anti-platelet; xanthoangelol