title: Structural characterization and stimulating effect on osteoblast differentiation of a purified heteropolysaccharide isolated from Hedysarum polybotrys.
abstract: Radix Hedysari polysaccharides (HPS) is the principal active fraction of Radix Hedysari (RH). The information about HPS3d, the main fraction of HPS3, and its effect on bone is still unknown. In the present study, the purified HPS3d was obtained by anion-exchange column. It consisted of 94.38% polysaccharide, 3.40% protein and 13.30% uronic acid. The molecular weight was measured to be 84.6kDa. The backbone consisted of galactopyranose and galacturonopyranose, and the side chains were composed of glucopyranose, rhamnopyranose and arabinofuranose. The FT-IR and elemental analysis showed that HPS3d was the sulfated polysaccharide. HPS3d upregulated alkaline phosphatase (ALP) activity and the expression of other osteogenic marker genes in osteoblast. In addition, HPS3d increased the expression and transcriptional activity of Runt-related transcription factor 2 (Runx-2) and Osterix, the two master genes of osteoblast differentiation. These findings suggest that HPS3d stimulates osteoblast differentiation by activation of Runx-2 and Osterix.
authors.affiliation: The Second Clinical Medical College of Lanzhou University, 82 Cuiying Men, Lanzhou 730030, PR China; Orthopaedics Key Laboratory of Gansu Province, Lanzhou 730000, PR China.
authors.forename: Lianggong
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy, Lanzhou University Second Hospital, Lanzhou 730000, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Orthopaedics Department Ward 1, Lanzhou University Second Hospital, Lanzhou 730000, PR China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Sheng
authors.affiliation: Food quality supervision and Inspection Institute of Hunan Province, Changsha 410000, PR China.
authors.forename: Tongqiang
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Lanzhou University First Hospital, Lanzhou 730000, PR China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Dang
authors.affiliation: Orthopaedics Key Laboratory of Gansu Province, Lanzhou 730000, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: An
authors.affiliation: Orthopaedics Key Laboratory of Gansu Province, Lanzhou 730000, PR China.
authors.forename: Jinglin
authors.initials: J
authors.lastname: Ma
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Shilan
authors.initials: S
authors.lastname: Feng
authors.affiliation: Orthopaedics Key Laboratory of Gansu Province, Lanzhou 730000, PR China; Department of Pharmacy, Lanzhou University Second Hospital, Lanzhou 730000, PR China. Electronic address: xiayy@lzu.edu.cn.
authors.forename: Yayi
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D014574:Uronic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D010940:Plant Proteins; D011134:Polysaccharides; D014574:Uronic Acids; D005690:Galactose
keywords: Osteoblast differentiation; Osterix; Radix Hedysari polysaccharides (HPS); Runx-2