title: Impacts of agricultural irrigation on nearby freshwater ecosystems: the seasonal influence of triazine herbicides in benthic algal communities.
abstract: A small hydrological basin (Lerma, NE Spain), transformed from its natural state (steppe) to rain-fed agriculture and recently to irrigation agriculture, has been monitored across four seasons of an agricultural year. The goal of this study was to assess how and whether agricultural activities impacted the nearby freshwater ecosystems via runoff. Specifically, we assessed the toxicity of three triazine herbicides, terbuthylazine, atrazine and simazine on the photosynthetic efficiency and structure of algal benthic biofilms (i.e., phototropic periphyton) in the small creek draining the basin. It was expected that the seasonal runoff of the herbicides in the creek affected the sensitivity of the periphyton in accord with the rationale of the Pollution Induced Community Tolerance (PICT): the exposure of the community to pollutants result in the replacement of sensitive species by more tolerant ones. In this way, PICT can serve to establish causal linkages between pollutants and the observed biological impacts. The periphyton presented significantly different sensitivities against terbuthylazine through the year in accord with the seasonal application of this herbicide in the crops nowadays. The sensitivity of already banned herbicides, atrazine and simazine does not display a clear seasonality. The different sensitivities to herbicides were in agreement with the expected exposures scenarios, according to the agricultural calendar, but not with the concentrations measured in water, which altogether indicates that the use of PICT approach may serve for long-term monitoring purposes. That will provide not only causal links between the occurrence of chemicals and their impacts on natural communities, but also information about the occurrence of chemicals that may escape from traditional sampling methods (water analysis). In addition, the EC50 and EC10 of periphyton for terbuthylazine or simazine are the first to be published and can be used for impact assessments.
authors.affiliation: Pyrenean Institute of Ecology (CSIC), Av. Montañana 1005, Zaragoza 50059, Spain; Biologisk Institut, NordCEE, Syddansk Universitet, Campusvej 55, 5230 Odense, Denmark.
authors.forename: Carmen
authors.initials: C
authors.lastname: Lorente
authors.affiliation: Geological Survey of Spain, C/Manuel Lasala no. 44, 50006 Zaragoza, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Causapé
authors.affiliation: Biologisk Institut, NordCEE, Syddansk Universitet, Campusvej 55, 5230 Odense, Denmark.
authors.forename: Ronnie N
authors.initials: RN
authors.lastname: Glud
authors.affiliation: Biologisk Institut, NordCEE, Syddansk Universitet, Campusvej 55, 5230 Odense, Denmark.
authors.forename: Kasper
authors.initials: K
authors.lastname: Hancke
authors.affiliation: Geological Survey of Spain, C/Manuel Lasala no. 44, 50006 Zaragoza, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Merchán
authors.affiliation: Pyrenean Institute of Ecology (CSIC), Av. Montañana 1005, Zaragoza 50059, Spain.
authors.forename: Selene
authors.initials: S
authors.lastname: Muñiz
authors.affiliation: Pyrenean Institute of Ecology (CSIC), Av. Montañana 1005, Zaragoza 50059, Spain.
authors.forename: Jonatan
authors.initials: J
authors.lastname: Val
authors.affiliation: Pyrenean Institute of Ecology (CSIC), Av. Montañana 1005, Zaragoza 50059, Spain. Electronic address: enrique.navarro@ipe.csic.es.
authors.forename: Enrique
authors.initials: E
authors.lastname: Navarro
mesh_terms: D057914:Agricultural Irrigation / Q000379:methods*; D001280:Atrazine / Q000633:toxicity*; D017753:Ecosystem; D006540:Herbicides / Q000633:toxicity*; D058086:Microalgae / Q000145:classification / Q000187:drug effects* / Q000254:growth & development; D010788:Photosynthesis; D012621:Seasons; D013030:Spain; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D006540:Herbicides; D014874:Water Pollutants, Chemical; D001280:Atrazine
keywords: Atrazine; Community tolerance; Periphyton; Photosynthetic efficiency; Simazine; Terbuthylazine