title: Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa.
abstract: The anti-quorum sensing activities towards the bacterium Pseudomonas aeruginosa PA01 (pyocyanin production, biofilm formation and twitching and flagella motility) of two crude extracts (methanol and acetone) of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) were evaluated in vitro for the first time. Both extracts demonstrated P. aeruginosa pyocyanin inhibitory activity, reducing its production for 49.90% and 42.44%, respectively. In addition, they both showed higher anti-biofilm activity (48.29% and 53.99%, respectively) than ampicillin (30.84%). Finally, O. rotunda extracts effectively reduced twitching and flagella motility of P. aeruginosa. Taken all together, these results suggest that endemic sponge species from the oldest lake in Europe may offer novel bioactive natural products with promising medicinal potential towards P. aeruginosa infections.
authors.affiliation: a Department of Life Sciences , Institute for Multidisciplinary Research (IMSI), University of Belgrade , Belgrade , Serbia.
authors.forename: Boris
authors.initials: B
authors.lastname: Pejin
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Talevska
authors.forename: Ana
authors.initials: A
authors.lastname: Ciric
authors.forename: Jasmina
authors.initials: J
authors.lastname: Glamoclija
authors.forename: Milos
authors.initials: M
authors.lastname: Nikolic
authors.forename: Trajce
authors.initials: T
authors.lastname: Talevski
authors.forename: Marina
authors.initials: M
authors.lastname: Sokovic
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects; D005060:Europe; D011161:Porifera / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D011710:Pyocyanine / Q000096:biosynthesis; D053038:Quorum Sensing / Q000187:drug effects*
chemical_list: D011710:Pyocyanine
keywords: Lake Ohrid; Ochridaspongia rotunda; Porifera; bioactivity