title: Novobiocin sensitivity of Salmonella typhimurium dam and/or seqA mutants.
abstract: This study was carried out to determine the effects of novobiocin, a gyrase inhibitor, on the growth, survival, motility and whole cell proteins of S. Typhimurium dam and/or seqA strains. Our results showed that the dam and seqA/dam mutants are the most sensitive to novobiocin, compared to wild type and seqA strains. Surprisingly, the motility of seqA mutants increased after exposure to novobiocin only in stationary phase cells. All the other strains showed a significant decrease in their motility. The analysis of protein profiles of all strains demonstrated several modifications as manifested by the alteration of the expression levels of certain bands. Our work is therefore of great interest in understanding the effects of novobiocin on S. Typhimurium and the involvement of DNA methylation.
authors.forename: Abdelwaheb
authors.initials: A
authors.lastname: Chatt
authors.forename: Meriem
authors.initials: M
authors.lastname: Aloui
authors.forename: Jihen
authors.initials: J
authors.lastname: Tagourti
authors.forename: Mouadh
authors.initials: M
authors.lastname: Mihoub
authors.forename: Ahmed
authors.initials: A
authors.lastname: Landoulsi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009675:Novobiocin / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D009675:Novobiocin