title: Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-ε-caprolactone nanofibers and growth factors for prevention of incisional hernia formation.
abstract: Incisional hernia affects up to 20% of patients after abdominal surgery. Unlike other types of hernia, its prognosis is poor, and patients suffer from recurrence within 10 years of the operation. Currently used hernia-repair meshes do not guarantee success, but only extend the recurrence-free period by about 5 years. Most of them are nonresorbable, and these implants can lead to many complications that are in some cases life-threatening. Electrospun nanofibers of various polymers have been used as tissue scaffolds and have been explored extensively in the last decade, due to their low cost and good biocompatibility. Their architecture mimics the natural extracellular matrix. We tested a biodegradable polyester poly-ε-caprolactone in the form of nanofibers as a scaffold for fascia healing in an abdominal closure-reinforcement model for prevention of incisional hernia formation. Both in vitro tests and an experiment on a rabbit model showed promising results.
authors.affiliation: Institute of Biophysics, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Plencner
authors.affiliation: Department of Surgery, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: East
authors.affiliation: Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic.
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Tonar
authors.affiliation: Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University in Prague, Prague, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Otáhal
authors.affiliation: Institute of Biophysics, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Prosecká
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; University Center for Energy Efficient Buildings, Czech Technical University in Prague, Buštěhrad, Czech Republic.
authors.forename: Michala
authors.initials: M
authors.lastname: Rampichová
authors.affiliation: Department of Surgery, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Krejčí
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; Department of Breeding and Zoohygiene of Laboratory Animals, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Andrej
authors.initials: A
authors.lastname: Litvinec
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; University Center for Energy Efficient Buildings, Czech Technical University in Prague, Buštěhrad, Czech Republic.
authors.forename: Matej
authors.initials: M
authors.lastname: Buzgo
authors.affiliation: Institute of Biophysics, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; University Center for Energy Efficient Buildings, Czech Technical University in Prague, Buštěhrad, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Míčková
authors.affiliation: Department of Surgery and Orthopedics, Small Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science Brno, Central European Institute of Technology, Brno, Czech Republic.
authors.forename: Alois
authors.initials: A
authors.lastname: Nečas
authors.affiliation: Department of Surgery, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Hoch
authors.affiliation: Institute of Biophysics, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic.
authors.forename: Evžen
authors.initials: E
authors.lastname: Amler
mesh_terms: D016475:3T3 Cells; D000005:Abdomen / Q000601:surgery; D058107:Abdominal Wound Closure Techniques / Q000295:instrumentation*; D000818:Animals; D001696:Biomechanical Phenomena; D048091:Guided Tissue Regeneration; D006547:Hernia / Q000517:prevention & control*; D006651:Histocytochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry / Q000627:therapeutic use*; D011091:Polyesters / Q000737:chemistry / Q000627:therapeutic use*; D011126:Polypropylenes / Q000737:chemistry / Q000627:therapeutic use*; D011183:Postoperative Complications / Q000517:prevention & control*; D011817:Rabbits; D013526:Surgical Mesh; D014945:Wound Healing / Q000187:drug effects
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D011091:Polyesters; D011126:Polypropylenes; C016240:polycaprolactone
keywords: growth factors; hernia regeneration; in vivo; nanofibers; surgical mesh