title: Characterization and genome sequence of Dev2, a new T7-like bacteriophage infecting Cronobacter turicensis.
abstract: Cronobacter spp. are opportunistic pathogenic bacteria that are responsible for severe infections in neonates. Powdered infant formula was confirmed to be the source in some cases. Bacteriophages offer a safe means for eliminating this pathogen. In the present study, we investigated the growth parameters and genome organization of a new bacteriophage, Dev2, isolated from sewage. The Dev2 phage contains DNA with a length of 39 kb and belongs to the T7 branch of the subfamily Autographivirinae, with the highest degree of identity to the phage K1F. The host specificity of Dev2 is limited to C. turicensis strains of the CT O:1 serotype. With a lower efficiency, this phage also infects some Salmonella and E. coli strains. The Dev2 phage can inactivate sensitive Cronobacter strains in reconstituted milk formula. The results obtained in this study are an important prerequisite for application of Dev2 in food control.
authors.affiliation: Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina 1, 84215, Bratislava, Slovakia.
authors.forename: Michal
authors.initials: M
authors.lastname: Kajsík
authors.forename: Lucia
authors.initials: L
authors.lastname: Oslanecová
authors.forename: Tomáš
authors.initials: T
authors.lastname: Szemes
authors.forename: Michalea
authors.initials: M
authors.lastname: Hýblová
authors.forename: Andrea
authors.initials: A
authors.lastname: Bilková
authors.forename: Hana
authors.initials: H
authors.lastname: Drahovská
authors.forename: Ján
authors.initials: J
authors.lastname: Turňa
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D059126:Cronobacter / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017902:Podoviridae / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*