title: Discovery of lipids from B. longum subsp. infantis using whole cell MALDI analysis.
abstract: Bifidobacteria are dominant members of the microbial community in the intestinal tract of infants, and studies have shown that glycolipids extracted from the cell surface of these bacteria elicit beneficial immune responses. Accordingly, the identification and structural characterization of glycolipids from the cell wall of bifidobacteria is the first step in correlating glycolipid structure with biological activity. Using whole cell MALDI as a screening tool, we herein present for the first time the identification and structural elucidation of the major polar lipids from Bifidobacterium longum subs. infantis. The lipids identified include an unprecedented plasmenyl cyclophosphatidic acid and a mixed acetal glycolipid, with the latter subsequently being isolated and found to suppress the innate immune response.
authors.affiliation: School of Chemical and Physical Sciences, Victoria University of Wellington , P.O. Box 600, Wellington 6140, New Zealand.
authors.forename: Mattie S M
authors.initials: MS
authors.lastname: Timmer
authors.forename: Janelle
authors.initials: J
authors.lastname: Sauvageau
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Foster
authors.forename: Jason
authors.initials: J
authors.lastname: Ryan
authors.forename: Kirill
authors.initials: K
authors.lastname: Lagutin
authors.forename: Odette
authors.initials: O
authors.lastname: Shaw
authors.forename: Jacquie L
authors.initials: JL
authors.lastname: Harper
authors.forename: Ian M
authors.initials: IM
authors.lastname: Sims
authors.forename: Bridget L
authors.initials: BL
authors.lastname: Stocker
mesh_terms: D001422:Bacterial Adhesion / Q000276:immunology; D001644:Bifidobacterium / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007422:Intestines / Q000737:chemistry* / Q000276:immunology* / Q000382:microbiology*; D008055:Lipids / Q000737:chemistry* / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013415:Sulfalene / Q000737:chemistry*
chemical_list: D006017:Glycolipids; D008055:Lipids; D013415:Sulfalene