title: Microcystin production and regulation under nutrient stress conditions in toxic microcystis strains.
abstract: Microcystin is a common and well-known cyanobacterial toxin whose intracellular role is still under investigation. Increasing knowledge on microcystin gene expression and regulation can contribute to the understanding of its putative cellular function. In this work, reverse transcription-quantitative PCR (RT-qPCR) was used to investigate the transcriptional response of the mcyD gene to nitrogen (nitrate and ammonium) and phosphorus limitation in two toxic Microcystis strains. The existence of a direct correlation between transcripts of mcyD and ntcA genes was also identified. In previous studies, NtcA (global nitrogen regulator) has been described as a potential component in the control of microcystin biosynthesis. This research showed that stress agents linked to nutrient deprivation could lead to a significant increase of microcystin production in both strains studied. The more toxic strain proved to be more resistant to nutrient limitation. The similar outcomes of mcyD regulation observed for all nutrients suggest that this response can be linked to oxidative stress of cells undergoing adverse growth conditions.
authors.affiliation: Department of Botany, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Juliana S M
authors.initials: JS
authors.lastname: Pimentel
authors.affiliation: Department of Botany, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil agiani@icb.ufmg.br.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Giani
mesh_terms: D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D052998:Microcystins / Q000378:metabolism*; D046931:Microcystis / Q000378:metabolism / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism; D018384:Oxidative Stress; D010758:Phosphorus / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological; D014158:Transcription, Genetic
chemical_list: D003470:Culture Media; D052998:Microcystins; D010758:Phosphorus; C078588:microcystin; D009584:Nitrogen