title: Biotechnological approach towards a highly efficient production of natural prostaglandins.
abstract: Prostaglandins (PGs) act as potent local hormones in nearly all tissues of the human body and are used for various medical applications. Heterologous expression of PG endoperoxide H-synthase from the alga, Gracilaria vermiculophylla, into E. coli and the application of this strain in biotransformation experiments resulted in a highly efficient conversion of arachidonic acid (ARA) yielding up to 130 mg natural PGs l(-1) in a laboratory scale approach. Detailed analyses of the products and production kinetics were performed, confirming a rapid conversion of ARA to PGs.
authors.affiliation: Biochemical Engineering, DECHEMA Research Institute, Theodor-Heuss-Allee 25, 60486, Frankfurt/Main, Germany.
authors.forename: J C
authors.initials: JC
authors.lastname: Guder
authors.forename: M
authors.initials: M
authors.lastname: Buchhaupt
authors.forename: I
authors.initials: I
authors.lastname: Huth
authors.forename: A
authors.initials: A
authors.lastname: Hannappel
authors.forename: N
authors.initials: N
authors.lastname: Ferreirós
authors.forename: G
authors.initials: G
authors.lastname: Geisslinger
authors.forename: J
authors.initials: J
authors.lastname: Schrader
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D044724:Gracilaria / Q000201:enzymology / Q000235:genetics; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics / Q000378:metabolism; D011453:Prostaglandins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*
chemical_list: D011453:Prostaglandins; D016718:Arachidonic Acid; D011451:Prostaglandin-Endoperoxide Synthases