title: [Cardiac ischemia in type 2 diabetes mellitus rats induced by high sucrose and high fat diet and STZ treated].
abstract: OBJECTIVE
  To build a type 2 diabetes mellitus rat model with cardiac ischemia.
  
  
  METHODS
  Male Wistar rats were fed high sucrose and high fat diet for four weeks and then injected with streptozoticin (STZ) (40 mg/kg .i.p.). The levels of fasting blood glucose and serum insulin were monitored every week. The body weights of rats were also measured every week. The blood levels of creatine kinase and lactate dehydrogenase (LDH) were measured following the electrocardiograph used BL-410 biological experiment system.
  
  
  RESULTS
  The serum insulin levels of diabetic rats were 4.05 ng/ml after four weeks high sucrose and high fat diet. The fasting blood glucose levels of diabetic rats were 17.9 mmol/L after injection. Compared with normal group, there was obvious change of S-T segment in the electrocardiograph of diabetic group at the fourteenth week. The levels of creatine kinase and lactate dehydrogenase in diabetic group significantly increased in comparison with those in normal group.
  
  
  CONCLUSIONS
  The cardiac ischemia of diabetic rats model is suitable for investigating cardiac disease of diabetes mellitus.
authors.forename: Xue-Li
authors.initials: XL
authors.lastname: Yao
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Zhang
authors.forename: Xiao-Liang
authors.initials: XL
authors.lastname: Wang
authors.forename: Hui-Rong
authors.initials: HR
authors.lastname: Liu
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003924:Diabetes Mellitus, Type 2 / Q000139:chemically induced / Q000503:physiopathology*; D059305:Diet, High-Fat / Q000009:adverse effects*; D019422:Dietary Sucrose / Q000009:adverse effects*; D004195:Disease Models, Animal; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D017202:Myocardial Ischemia / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
chemical_list: D019422:Dietary Sucrose; D013311:Streptozocin; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase