title: Artificial enzymes with protein scaffolds: structural design and modification.
abstract: Recent development in biochemical experiment techniques and bioinformatics has enabled us to create a variety of artificial biocatalysts with protein scaffolds (namely 'artificial enzymes'). The construction methods of these catalysts include genetic mutation, chemical modification using synthetic molecules and/or a combination of these methods. Designed evolution strategy based on the structural information of host proteins has become more and more popular as an effective approach to construct artificial protein-based biocatalysts with desired reactivities. From the viewpoint of application of artificial enzymes for organic synthesis, recently constructed artificial enzymes mediating oxidation, reduction and C-C bond formation/cleavage are introduced in this review article.
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology (NAIST), Ikoma, Nara 630-0192, Japan. Electronic address: tmatsuo@ms.naist.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuo
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology (NAIST), Ikoma, Nara 630-0192, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Hirota
mesh_terms: D055162:Biocatalysis; D019020:Directed Molecular Evolution; D004798:Enzymes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011487:Protein Conformation
chemical_list: D004798:Enzymes
keywords: Artificial enzyme; Biocatalyst; C–C bond formation/cleavage; Oxidation; Reduction