title: Protective effect of inhalation of hydrogen gas on radiation-induced dermatitis and skin injury in rats.
abstract: The effect of inhalation of hydrogen-containing gas (1.3% hydrogen + 20.8% oxygen + 77.9% nitrogen) (HCG) on radiation-induced dermatitis and on the healing of healing-impaired skin wounds in rats was examined using a rat model of radiation-induced skin injury. An X-ray dose of 20 Gy was irradiated onto the lower part of the back through two holes in a lead shield. Irradiation was performed before or after inhalation of HCG for 2 h. Inhalation of HCG significantly reduced the severity of radiodermatitis and accelerated healing-impaired wound repair. Staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) and 8-hydroxy-2(')-deoxyguanosine (8-OHdG) showed that the proportion of apoptotic keratinocytes and the level of staining in the X-irradiated skin of rats that pre-inhaled HCG were significantly lower than that of rats which did not pre-inhale HCG. Cutaneous full-thickness wounds were then created in the X-irradiated area to examine the time-course of wound healing. X-irradiation significantly increased the time required for wound healing, but the inhalation of HCG prior to the irradiation significantly decreased the delay in wound healing compared with the control and post-inhalation of HCG groups. Therefore, radiation-induced skin injury can potentially be alleviated by the pre-inhalation of HCG.
authors.affiliation: Department of Radiology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Sadahiro
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: Division of Environmental Medicine, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan Second Division, Aeromedical Laboratory, Japan Air Self-Defense Force, 1-2-10 Sakae, Tachikawa, Tokyo 190-8585, Japan fujitama@ndmc.ac.jp bxb01424@nifty.com.
authors.forename: Masanori
authors.initials: M
authors.lastname: Fujita
authors.affiliation: Division of Biomedical Engineering, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.affiliation: Division of Environmental Medicine, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Tachibana
authors.affiliation: Division of Environmental Medicine, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Yoritsuna
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Department of Radiology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Tatsumi
authors.initials: T
authors.lastname: Kaji
authors.affiliation: Department of Radiology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Kawauchi
authors.affiliation: Department of Health Crisis Management, National Institute of Public Health, 2-3-6 Minami, Wako, Saitama 351-0197, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kanatani
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D005740:Gases; D006859:Hydrogen / Q000008:administration & dosage*; D008297:Male; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D011855:Radiodermatitis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000528:radiation effects*; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D000975:Antioxidants; D005740:Gases; D011837:Radiation-Protective Agents; D006859:Hydrogen
keywords: antioxidant; healing-impaired wound; hydrogen; radiodermatitis; radioprotection