title: The effect of Pleurotus ostreatus arabinofuranosidase and its evolved variant in lignocellulosic biomasses conversion.
abstract: The fungal arabinofuranosidase from Pleurotus ostreatus PoAbf recombinantly expressed in Pichia pastoris rPoAbf and its evolved variant rPoAbf F435Y/Y446F were tested for their effectiveness to enhance the enzymatic saccharification of three lignocellulosic biomasses, namely Arundo donax, corn cobs and brewer's spent grains (BSG), after chemical or chemical-physical pretreatment. All the raw materials were subjected to an alkaline pretreatment by soaking in aqueous ammonia solution whilst the biomass from A. donax was also pretreated by steam explosion. The capability of the wild-type and mutant rPoAbf to increase the fermentable sugars recovery was assessed by using these enzymes in combination with different (hemi)cellulolytic activities. These enzymatic mixtures were either entirely of commercial origin or contained the cellulase from Streptomyces sp. G12 CelStrep recombinantly expressed in Escherichia coli in substitution to the commercial counterparts. The addition of the arabinofuranosidases from P. ostreatus improved the hydrolytic efficiency of the commercial enzymatic cocktails on all the pretreated biomasses. The best results were obtained using the rPoAbf evolved variant and are represented by increases of the xylose recovery up to 56.4%. These data clearly highlight the important role of the accessory hemicellulolytic activities to optimize the xylan bioconversion yields.
authors.affiliation: Institute of Biosciences and BioResources, National Research Council, Napoli, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Marcolongo
authors.affiliation: Institute of Biosciences and BioResources, National Research Council, Napoli, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Ionata
authors.affiliation: Institute of Biosciences and BioResources, National Research Council, Napoli, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: La Cara
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Napoli, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Amore
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Napoli, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Giacobbe
authors.affiliation: Department of Agriculture, University of Naples "Federico II", Portici (Napoli), Italy.
authors.forename: Olimpia
authors.initials: O
authors.lastname: Pepe
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Napoli, Italy. Electronic address: vfaraco@unina.it.
authors.forename: Vincenza
authors.initials: V
authors.lastname: Faraco
mesh_terms: D018533:Biomass; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D010843:Pichia / Q000201:enzymology / Q000235:genetics; D020076:Pleurotus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics
chemical_list: C036909:lignocellulose; D008031:Lignin; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase
keywords: Alkaline pretreatment; Arabinofuranosidase; Lignocellulose; Pleurotus ostreatus; Saccharification; Steam explosion