title: Increasing magnetite contents of polymeric magnetic particles dramatically improves labeling of neural stem cell transplant populations.
abstract: Safe and efficient delivery of therapeutic cells to sites of injury/disease in the central nervous system is a key goal for the translation of clinical cell transplantation therapies. Recently, 'magnetic cell localization strategies' have emerged as a promising and safe approach for targeted delivery of magnetic particle (MP) labeled stem cells to pathology sites. For neuroregenerative applications, this approach is limited by the lack of available neurocompatible MPs, and low cell labeling achieved in neural stem/precursor populations. We demonstrate that high magnetite content, self-sedimenting polymeric MPs [unfunctionalized poly(lactic acid) coated, without a transfecting component] achieve efficient labeling (≥90%) of primary neural stem cells (NSCs)-a 'hard-to-label' transplant population of major clinical relevance. Our protocols showed high safety with respect to key stem cell regenerative parameters. Critically, labeled cells were effectively localized in an in vitro flow system by magnetic force highlighting the translational potential of the methods used.
authors.affiliation: Cellular and Neural Engineering Group, Institute for Science and Technology in Medicine, Keele University, Keele, Staffordshire, United Kingdom.
authors.forename: Christopher F
authors.initials: CF
authors.lastname: Adams
authors.affiliation: Department of Surgery, Drexel University College of Medicine, Philadelphia, PA, USA.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Rai
authors.affiliation: Department of Chemical Engineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, United Kingdom.
authors.forename: Gregor
authors.initials: G
authors.lastname: Sneddon
authors.affiliation: Department of Chemical Engineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, United Kingdom.
authors.forename: Humphrey H P
authors.initials: HH
authors.lastname: Yiu
authors.affiliation: Department of Surgery, Drexel University College of Medicine, Philadelphia, PA, USA; Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA, USA. Electronic address: bpolyak@drexelmed.edu.
authors.forename: Boris
authors.initials: B
authors.lastname: Polyak
authors.affiliation: Cellular and Neural Engineering Group, Institute for Science and Technology in Medicine, Keele University, Keele, Staffordshire, United Kingdom. Electronic address: d.chari@keele.ac.uk.
authors.forename: Divya M
authors.initials: DM
authors.lastname: Chari
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D050997:Nanomedicine / Q000379:methods*; D058953:Neural Stem Cells / Q000166:cytology*; D009474:Neurons / Q000378:metabolism; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D012038:Regeneration; D033581:Stem Cell Transplantation
chemical_list: D001672:Biocompatible Materials; D058185:Magnetite Nanoparticles; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)
keywords: Labeling; Magnetic cell targeting; Neural stem cells; Polymeric magnetic particles; Transplant cells