title: Induction of CYP1A and ABC transporters in European sea bass (Dicentrarchus labrax) upon 2,3,7,8-TCDD waterborne exposure.
abstract: The aim of this study was to characterize the responsiveness of CYP1A and ABC transport proteins in European Sea bass (Dicentrarchus labrax) waterborne exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) (46 pg/L) for 24 h and 7 days. Genes modulation (abcb1, abcc1-2, cyp1a), EROD activity were investigated in liver and 2,3,7,8-TCDD bioconcentration in liver and muscle. TCDD induced significantly cyp1a gene expression and EROD activity at 24 h and 7 d. A significant up-regulation of abcb1 was also observed but only after 7 days. No modulation of abcc1 and abcc2 genes was observed. Waterborne TCDD exposure was able to induce CYP1A and abcb1 encoding for P-glycoprotein in juvenile of European sea bass.
authors.affiliation: Department of Physical Earth and Environmental Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy. Electronic address: dellatorre2@unisi.it.
authors.forename: Camilla
authors.initials: C
authors.lastname: Della Torre
authors.affiliation: Department of Physical Earth and Environmental Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Mariottini
authors.affiliation: Department of Physical Earth and Environmental Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Vannuccini
authors.affiliation: Department of Physical Earth and Environmental Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Trisciani
authors.affiliation: Department of Physical Earth and Environmental Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Marchi
authors.affiliation: Department of Physical Earth and Environmental Sciences, University of Siena, Via Mattioli 4, 53100 Siena, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Corsi
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001492:Bass / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D060888:Real-Time Polymerase Chain Reaction; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D000072317:Polychlorinated Dibenzodioxins; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1
keywords: ABC transport proteins; CYP1A; Detoxification; European sea bass; TCDD