title: A novel, simplified technique to amplify Eimeria (Coccidia: Apicomplexa) DNA from oocysts.
abstract: A new method to amplify coccidia DNA by polymerase chain reaction (PCR) was developed by placing freeze-thawed oocysts in Ready-to-Go PCR bead tubes and using a 5-min initial heat denaturation step. Positive PCR reactions were found in 3 of 3 samples containing 20 or 50 oocysts; when ≤5 oocysts were used, 1 of 3 samples was positive. This technique shows potential for effectively and efficiently detecting and identifying oocysts from soil, feces, and other matter.
authors.affiliation: Department of Poultry Science, University of Georgia, Athens, Georgia 30602;
authors.forename: R W
authors.initials: RW
authors.lastname: Gerhold
authors.forename: L R
authors.initials: LR
authors.lastname: McDougald
authors.forename: R B
authors.initials: RB
authors.lastname: Beckstead
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000175:diagnosis / Q000469:parasitology; D001717:Birds; D003048:Coccidiosis / Q000175:diagnosis / Q000469:parasitology; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification*; D004539:Eimeria / Q000235:genetics* / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D008863:Microspheres; D033661:Oocysts / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012987:Soil / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D012987:Soil