title: Expression of SGTA correlates with neuronal apoptosis and reactive gliosis after spinal cord injury.
abstract: Small glutamine-rich tetratricopeptide repeat (TPR)-containing protein alpha (SGTA) is a novel TPR-containing protein involved in various biological processes. However, the expression and roles of SGTA in the central nervous system remain unknown. We have produced an acute spinal cord injury (SCI) model in adult rats and found that SGTA protein levels first significantly increase, reach a peak at day 3 and then gradually return to normal level at day 14 after SCI. These changes are striking in neurons, astrocytes and microglia. Additionally, colocalization of SGTA/active caspase-3 has been detected in neurons and colocalization of SGTA/proliferating cell nuclear antigen has been detected in astrocytes and microglial. In vitro, SGTA depletion by short interfering RNA inhibits astrocyte proliferation and decreases cyclinA and cyclinD1 protein levels. SGTA knockdown also reduces neuronal apoptosis. We speculate that SGTA is involved in biochemical and physiological responses after SCI.
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Minhao
authors.initials: M
authors.lastname: Chen
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Xia
authors.forename: Xinhui
authors.initials: X
authors.lastname: Zhu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.forename: Dawei
authors.initials: D
authors.lastname: Xu
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Ni
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Shixian
authors.initials: S
authors.lastname: Yan
authors.forename: Xinghai
authors.initials: X
authors.lastname: Cheng
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Liu
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D059447:Cell Cycle Checkpoints; D049109:Cell Proliferation; D005455:Fluorescent Antibody Technique; D055785:Gene Knockdown Techniques; D005911:Gliosis / Q000209:etiology* / Q000473:pathology; D008297:Male; D018832:Molecular Chaperones; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D010641:Phenotype; D034741:RNA, Small Interfering / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000150:complications* / Q000473:pathology
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D018832:Molecular Chaperones; D034741:RNA, Small Interfering; C512694:Sgta protein, rat