title: Enhancing extracellular lipolytic enzyme production in an arctic bacterium, Psychrobacter sp. ArcL13, by using statistical optimization and fed-batch fermentation.
abstract: A strain isolated from seawater samples in the Chuckchi Sea and exhibiting extracellular lipolytic activity was identified using 16S rRNA gene sequence analysis as Psychrobacter sp. ArcL13. The lipolytic enzyme exhibited cold-active properties and high hydrolytic activity toward p-nitrophenyl caprylate (C8), p-nitrophenyl decanoate (C10), and sunflower oil. Statistical optimization of the medium components was performed to enhance the production of cold-active extracellular lipolytic activity. Glucose, yeast extract (YE), and NaCl were selected as the main efficient nutrient sources. Fed-batch fermentation using optimized medium with concentrated YE as the main feeding material showed a maximum lipolytic activity of 10.7 U/mL, which was a 21-fold increase in production over unoptimized flask culture conditions. The information obtained in the present study could prove applicable to the production of cold-active lipase on a large scale.
authors.affiliation: a Division of Life Sciences , Korea Polar Research Institute , KIOST , Incheon , South Korea.
authors.forename: Sunghui
authors.initials: S
authors.lastname: Kim
authors.forename: Ah Ram
authors.initials: AR
authors.lastname: Wi
authors.forename: Ha Ju
authors.initials: HJ
authors.lastname: Park
authors.forename: Dockyu
authors.initials: D
authors.lastname: Kim
authors.forename: Han-Woo
authors.initials: HW
authors.lastname: Kim
authors.forename: Joung Han
authors.initials: JH
authors.lastname: Yim
authors.forename: Se Jong
authors.initials: SJ
authors.lastname: Han
mesh_terms: D001110:Arctic Regions; D061249:Batch Cell Culture Techniques / Q000379:methods; D056808:Biostatistics / Q000379:methods*; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002210:Caprylates / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D044096:Psychrobacter / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D002210:Caprylates; D003470:Culture Media; D004798:Enzymes; D012336:RNA, Ribosomal, 16S; C034106:4-nitrophenyloctanoate; D002244:Carbon; D002265:Carboxylic Ester Hydrolases; D009584:Nitrogen
keywords: arctic bacterium; fed-batch fermentation; lipolytic activity; psychrophile; statistical optimization