title: The effect of patient age on bone formation using a fully synthetic nanocrystalline bone augmentation material in maxillary sinus grafting.
abstract: OBJECTIVE
  Maxillary sinus floor augmentation is a treatment that has been proposed for patients in whom the alveolar bone height is insufficient. This procedure is commonly used in patients aged 40 to 70 years and older. However, little information exists whether the factor of age might influence the outcome of augmentation procedures. The aim of this study was to investigate whether the patient's age has an effect on bone formation and incorporation in maxillary sinus floor augmentation procedures.
  
  
  METHODS
  A fully synthetic nanocrystalline bone augmentation material (NanoBone, Artoss) was used for sinus floor augmentation in patients with a subantral vertical bone height of at least 3 mm and maximum of 7 mm. After 7 months healing time, biopsy specimens were taken and were divided into two groups according to the patient's age. Exclusion criteria were poor general health (eg, severe renal/and or liver disease), history of a radiotherapy in the head region, chemotherapy at the time of surgical procedure, noncompensated diabetes mellitus, symptoms of a maxillary sinus disease, active periodontal or systemic diseases, smoking, and poor oral hygiene. Histologic analyses with hematoxylin-eosin stain were performed. Multinucleated osteoclast-like cells were identified by histochemical staining (tartrate-resistant acid phosphatase [TRAP]). Quantitative and age-dependent assessment of bone formation, residual bone grafting material, and soft tissue formation following sinus augmentation was performed using histomorphometric analysis and the Bonferroni adjustment of the Student t test.
  
  
  RESULTS
  Twenty biopsy specimens from 17 patients were taken and divided into two groups according to age (group 1: 41 to 52 years; group 2: 66 to 71 years) containing 10 specimens each, which were analyzed in triplicate resulting in a total of 30 specimens per group. A regeneration process with varying amounts of newly formed bone surrounded by marrow-like tissue was present in all augmented regions. No signs of inflammation or immune reactions were visible. Residual particles of the augmentation material could be observed within the specimens. An age-dependent difference in investigated parameters between the two age groups could not be documented.
  
  
  CONCLUSIONS
  The histologic examinations confirm that the fully synthetic nanocrystalline bone augmentation material used in this study is biocompatible and allows maxillary sinus augmentation in patients aged 41 to 70 years.
authors.forename: Michael
authors.initials: M
authors.lastname: Wolf
authors.forename: Alexander
authors.initials: A
authors.lastname: Wurm
authors.forename: Friedhelm
authors.initials: F
authors.lastname: Heinemann
authors.forename: Thomas
authors.initials: T
authors.lastname: Gerber
authors.forename: Christoph
authors.initials: C
authors.lastname: Reichert
authors.forename: Andreas
authors.initials: A
authors.lastname: Jäger
authors.forename: Werner
authors.initials: W
authors.lastname: Götz
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D018786:Bone Substitutes / Q000008:administration & dosage*; D004338:Drug Combinations; D017886:Durapatite / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008297:Male; D008443:Maxillary Sinus; D008875:Middle Aged; D013516:Oral Surgical Procedures, Preprosthetic / Q000379:methods; D010012:Osteogenesis / Q000502:physiology*; D012822:Silicon Dioxide / Q000008:administration & dosage*; D059546:Sinus Floor Augmentation / Q000379:methods*; D014945:Wound Healing
chemical_list: D018786:Bone Substitutes; D004338:Drug Combinations; C534915:NanoBone; D012822:Silicon Dioxide; D017886:Durapatite