title: Bio-inspired cryo-ink preserves red blood cell phenotype and function during nanoliter vitrification.
abstract: Current red-blood-cell cryopreservation methods utilize bulk volumes, causing cryo-injury of cells, which results in irreversible disruption of cell morphology, mechanics, and function. An innovative approach to preserve human red-blood-cell morphology, mechanics, and function following vitrification in nanoliter volumes is developed using a novel cryo-ink integrated with a bioprinting approach.
authors.affiliation: Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, Stanford University School of Medicine, Palo Alto, CA, 94304, USA; Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Division for Biomedical Engineering, Division of Infectious Diseases, Renal Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA.
authors.forename: Rami
authors.initials: R
authors.lastname: El Assal
authors.forename: Sinan
authors.initials: S
authors.lastname: Guven
authors.forename: Umut Atakan
authors.initials: UA
authors.lastname: Gurkan
authors.forename: Irep
authors.initials: I
authors.lastname: Gozen
authors.forename: Hadi
authors.initials: H
authors.lastname: Shafiee
authors.forename: Sedef
authors.initials: S
authors.lastname: Dalbeyler
authors.forename: Noor
authors.initials: N
authors.lastname: Abdalla
authors.forename: Gawain
authors.initials: G
authors.lastname: Thomas
authors.forename: Wendy
authors.initials: W
authors.lastname: Fuld
authors.forename: Ben M W
authors.initials: BM
authors.lastname: Illigens
authors.forename: Jessica
authors.initials: J
authors.lastname: Estanislau
authors.forename: Joseph
authors.initials: J
authors.lastname: Khoory
authors.forename: Richard
authors.initials: R
authors.lastname: Kaufman
authors.forename: Claudia
authors.initials: C
authors.lastname: Zylberberg
authors.forename: Neal
authors.initials: N
authors.lastname: Lindeman
authors.forename: Qi
authors.initials: Q
authors.lastname: Wen
authors.forename: Ionita
authors.initials: I
authors.lastname: Ghiran
authors.forename: Utkan
authors.initials: U
authors.lastname: Demirci
mesh_terms: D000599:Amino Acids, Diamino / Q000737:chemistry; D001696:Biomechanical Phenomena; D062028:Bioprinting / Q000295:instrumentation / Q000379:methods*; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D007281:Ink; D042541:Intracellular Space / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D017463:Receptors, Complement 3b / Q000378:metabolism; D058989:Vitrification
chemical_list: D000599:Amino Acids, Diamino; C509991:CR1 protein, human; D017382:Reactive Oxygen Species; D017463:Receptors, Complement 3b; D009569:Nitric Oxide; C045628:ectoine
keywords: bio-inspired materials; bio-printing; cryoprotective agents; nanoliter vitrification; red blood cell cryopreservation