title: [MiR-124 suppresses the proliferation of human prostate cancer PC3 cells by targeting PKM2].
abstract: OBJECTIVE
  To explore the mechanism of miR-124 inhibiting the proliferative activity of prostate cancer PC3 cells.
  
  
  METHODS
  Luciferase reporter gene assay was used to examine the specific binding ability of miR-124 to PKM2 mRNA 3'-UTR. After miR-124 was transfected mimic to PC3 cells, the expression levels of PKM2 mRNA and protein were detected by real-time fluorescence quantitative PCR (qRT-PCR) and Western blot, respectively. The effects of miR-124 mimic and PKM2 siRNA on the proliferative activity of the PC3 cells were determined by MTT assay.
  
  
  RESULTS
  The expressions of PKM2 mRNA and protein were upregulated (5.12 +/- 0.35) times and (4.05 +/- 0.20) times respectively in the PC3 cells as compared with those in the RWPE-1 cells (P < 0.05). Luciferase reporter gene assay demonstrated that miR-124 targeted PKM2 3'-UTR. At 24 hours after transfection with miR-124 mimic, the PKM2 protein expression in the PC3 cells was downregulated (0.16 +/- 0.04) times (P < 0.05), while the PKM2 mRNA level was not changed significantly (P > 0.05), as compared with the control group. MTT assay showed that both miRNA-124 mimic and PKM2 siRNA could inhibit the proliferation of the PC3 cells, but the former exhibited a greater inhibitory effect than the latter. After transfection with miR-124 mimic and PKM2 siRNA, the cell growth rates were (66.20 +/- 5.10)% vs (82.10 +/- 6.35)% at 24 hours (P < 0.05) and (49.34 +/- 2.37)% vs (70.10 +/- 5.80)% at 48 hours (P < 0.05).
  
  
  CONCLUSIONS
  miR-124 can suppress the proliferation of PC3 cells by regulating the PKM2 gene.
authors.forename: Lei
authors.initials: L
authors.lastname: Lü
authors.forename: Jing-Dong
authors.initials: JD
authors.lastname: Yuan
authors.forename: Zuo-Liang
authors.initials: ZL
authors.lastname: Cao
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.forename: Chuan-Hua
authors.initials: CH
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Fu-Qing
authors.initials: FQ
authors.lastname: Zeng
mesh_terms: D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D013963:Thyroid Hormones / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D000097810:Thyroid Hormone-Binding Proteins
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D035683:MicroRNAs; D013963:Thyroid Hormones; D000097810:Thyroid Hormone-Binding Proteins; C509207:MIRN124 microRNA, human