title: Lichen scrofulosorum caused by Mycobacterium leprae: first report.
abstract: BACKGROUND
  Tuberculides are skin lesions caused by the hematogeneous dissemination of Mycobacterium tuberculosis. Bacilli are rapidly destroyed in the skin and are thus neither visible histologically nor identifiable by culture. Diagnosis depends on previous knowledge of systemic and/or cutaneous tuberculosis. Lichen scrofulosorum (LS), the most uncommon variant of tuberculid, is usually associated with M. tuberculosis infection of lymph nodes or bone but was also reported in association with other mycobacterioses.
  
  
  OBJECTIVE
  We report a case of LS in a patient with M. leprae infection.
  
  
  METHODS
  In 2008, a 51-year-old woman from the Philippines was diagnosed with tuberculoid leprosy and treated. In 2010 the leprosy was considered to have been cured, and treatment was stopped. In 2011 the patient presented with lesions on the trunk and legs. Biopsy specimens were obtained for histopathologic examination and DNA detection for polymerase chain reaction (PCR).
  
  
  RESULTS
  Histopathology in the biopsy from the trunk revealed the dermis to be diffusely occupied by granulomas with perineural and periadnexal disposition. Granulomas were composed of epithelioid cells and lymphocytes. Fite-Faraco staining revealed a few solid acid-fast bacilli within nerve fascicles. Reinfection or the re-reactivation of multibacillary borderline tuberculoid leprosy was diagnosed. Histopathology in the biopsy taken from the leg showed superficial, well-formed granulomas in the vicinity of hair follicles and sweat ducts. No acid-fast bacilli were seen. Analysis by PCR revealed M. leprae DNA in specimens from both the leg and trunk. The clinical features of the papular eruption and the histopathologic findings and concomitant mycobacterial infection with M. leprae led to a diagnosis of LS. Treatment was commenced with dapsone 100 mg/day, clofazimine 50 mg/day and 300 mg/month, and rifampicin 600 mg/day. The lichenoid eruption on the legs disappeared at one month of therapy, whereas the other skin lesions resolved in one year leaving residual hypochromic macules.
  
  
  CONCLUSIONS
  Infection with M. leprae may cause LS. The use of PCR in skin biopsies from granulomatous dermatitis of unknown origin can help to identify the responsible agents.
authors.affiliation: Unit of Social Dermatology and National Reference Center for Hansen's Disease, National Institute for Cancer Research, Genoa, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Fiallo
authors.forename: Francesco
authors.initials: F
authors.lastname: Cabiddu
authors.forename: Andrea
authors.initials: A
authors.lastname: Clapasson
authors.forename: Aurora
authors.initials: A
authors.lastname: Parodi
mesh_terms: D005260:Female; D006801:Humans; D007918:Leprosy / Q000150:complications*; D017512:Lichenoid Eruptions / Q000382:microbiology*; D008875:Middle Aged; D009166:Mycobacterium leprae; D014382:Tuberculosis, Cutaneous / Q000382:microbiology*