title: Parallel recognition of cancer cells using an addressable array of solid-state micropores.
abstract: Early stage detection and precise quantification of circulating tumor cells (CTCs) in the peripheral blood of cancer patients are important for early diagnosis. Early diagnosis improves the effectiveness of the therapy and results in better prognosis. Several techniques have been used for CTC detection but are limited by their need for dye tagging, low throughput and lack of statistical reliability at single cell level. Solid-state micropores can characterize each cell in a sample providing interesting information about cellular populations. We report a multi-channel device which utilized solid-state micropores array assembly for simultaneous measurement of cell translocation. This increased the throughput of measurement and as the cells passed the micropores, tumor cells showed distinctive current blockade pulses, when compared to leukocytes. The ionic current across each micropore channel was continuously monitored and recorded. The measurement system not only increased throughput but also provided on-chip cross-relation. The whole blood was lysed to get rid of red blood cells, so the blood dilution was not needed. The approach facilitated faster processing of blood samples with tumor cell detection efficiency of about 70%. The design provided a simple and inexpensive method for rapid and reliable detection of tumor cells without any cell staining or surface functionalization. The device can also be used for high throughput electrophysiological analysis of other cell types.
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, Arlington, TX 76019, USA; Department of Electrical Engineering, University of Texas at Arlington, Arlington, TX 76011, USA; Nanotechnology Research Center, University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Azhar
authors.initials: A
authors.lastname: Ilyas
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, Arlington, TX 76019, USA; Department of Electrical Engineering, University of Texas at Arlington, Arlington, TX 76011, USA; Nanotechnology Research Center, University of Texas at Arlington, Arlington, TX 76019, USA.
authors.forename: Waseem
authors.initials: W
authors.lastname: Asghar
authors.affiliation: Nanotechnology Research Center, University of Texas at Arlington, Arlington, TX 76019, USA; Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76010, USA.
authors.forename: Young-tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, Arlington, TX 76019, USA; Department of Electrical Engineering, University of Texas at Arlington, Arlington, TX 76011, USA; Nanotechnology Research Center, University of Texas at Arlington, Arlington, TX 76019, USA; Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76010, USA; Joint Graduate Committee of Bioengineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, University of Texas at Arlington, Arlington, TX 76010, USA; Department of Urology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA. Electronic address: smiqbal@uta.edu.
authors.forename: Samir M
authors.initials: SM
authors.lastname: Iqbal
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001932:Brain Neoplasms / Q000473:pathology / Q000556:secondary; D055088:Early Detection of Cancer; D004867:Equipment Design; D006461:Hemolysis; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology; D008851:Micropore Filters; D009369:Neoplasms / Q000097:blood* / Q000175:diagnosis*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*
keywords: CTC; Detection efficiency; Leukocytes; Pulse signal; Single-cell analysis; Single-cell measurement; Translocation profile