title: Analysis of the response of human keratinocytes to Malassezia globosa and restricta strains.
abstract: Malassezia spp. are saprophyte yeasts involved in skin diseases with different degrees of severity. The aim of our study was to analyze the response of human epidermal keratinocytes to Malassezia globosa and restricta strains evaluating the host defence mechanisms induced by Malassezia spp. colonization. Our results showed a different modulation of the inflammatory and immunomodulatory cytokine pathways obtained with the different strains of Malassezia tested. In addition, this expression is altered by blocking the TLR2 receptor. In comparison with M. furfur, M. globosa and restricta displayed an unexpected and striking cytotoxicity on keratinocytes. The differences observed could be related to the different modalities of interaction between keratinocytes and Malassezia strains, but also to their growth condition. Taken together, these results indicate that M. globosa or M. restricta colonization exert a different control on the cytokine inflammatory response activated in the human keratinocyte in which TLR2 might be involved. M. globosa and M. restricta may play a synergistic role in the exacerbation of skin diseases in which both are found.
authors.affiliation: Microbiology Section, Department of Experimental Medicine, Second University of Naples, Via Costantinopoli, 16, 80138, Naples, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Donnarumma
authors.forename: Brunella
authors.initials: B
authors.lastname: Perfetto
authors.forename: Iole
authors.initials: I
authors.lastname: Paoletti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Oliviero
authors.forename: Cécile
authors.initials: C
authors.lastname: Clavaud
authors.forename: Aurelia
authors.initials: A
authors.lastname: Del Bufalo
authors.forename: Audrey
authors.initials: A
authors.lastname: Guéniche
authors.forename: Roland
authors.initials: R
authors.lastname: Jourdain
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Tufano
authors.forename: Lionel
authors.initials: L
authors.lastname: Breton
mesh_terms: D017209:Apoptosis; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003881:Dermatomycoses / Q000276:immunology* / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology; D015603:Keratinocytes / Q000276:immunology* / Q000382:microbiology*; D008290:Malassezia / Q000254:growth & development*; D051195:Toll-Like Receptor 2 / Q000276:immunology
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2