title: Epigallocatechin-3-gallate suppresses the lipid deposition through the apoptosis during differentiation in bovine bone marrow mesenchymal stem cells.
abstract: Epigallocatechin gallate (EGCG), a major component of tea, has known effects on obesity, fatty liver, and obesity-related cancer. We explored the effects of EGCG on the differentiation of bovine mesenchymal stem cells (BMSCs, which are multipotent) in a dose- and time-dependent manner. Differentiating BMSCs were exposed to various concentrations of EGCG (0, 10, 50, 100, and 200 µM) for 2, 4, and 6 days. BMSCs were cultured in Dulbecco's modified Eagle's medium (DMEM)/high-glucose medium with adipogenic inducers for 6 days, and the expression levels of various genes involved in adipogenesis were measured using real-time polymerase chain reaction (PCR) and Western blotting. We assessed apoptosis by flow cytometry and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining of control and EGCG-exposed cells. We found that EGCG significantly suppressed fat deposition and cell viability (P < 0.05). The mRNA and protein levels of various adipogenic factors were measured. Expression of the genes encoding peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer-binding protein alpha (CEBPA), fatty acid-binding protein 4 (FABP4), and stearoyl-CoA desaturase (SCD) were diminished by EGCG during adipogenic differentiation (P < 0.05). We also found that EGCG lowered the expression levels of the adipogenic proteins encoded by these genes (P < 0.05). EGCG induced apoptosis during adipogenic differentiation (P < 0.05). Thus, exposure to EGCG potentially inhibits adipogenesis by triggering apoptosis; the data suggest that EGCG inhibits adipogenic differentiation in BMSCs.
authors.affiliation: Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, #564 Omockchun-dong, Suwon, 441-706, Republic of Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Jeong
authors.forename: Sekar
authors.initials: S
authors.lastname: Suresh
authors.forename: Mi
authors.initials: M
authors.lastname: Jang
authors.forename: Mi Na
authors.initials: MN
authors.lastname: Park
authors.forename: Kuppannan
authors.initials: K
authors.lastname: Gobianand
authors.forename: Seungkwon
authors.initials: S
authors.lastname: You
authors.forename: Sung-Heom
authors.initials: SH
authors.lastname: Yeon
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000166:cytology*; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D050556:Fatty Acid-Binding Proteins; D047495:PPAR gamma; D002392:Catechin; C045651:epigallocatechin gallate; D013230:Stearoyl-CoA Desaturase
keywords: BMSC; EGCG; adipogenic factor; apoptosis; differentiation