title: A comparative immunohistochemistry study of diagnostic tools in salivary gland tumors: usefulness of mammaglobin, gross cystic disease fluid protein 15, and p63 cytoplasmic staining for the diagnosis of mammary analog secretory carcinoma?
abstract: BACKGROUND
  Mammary analog secretory carcinoma (MASC) of the salivary gland has been recently described according to morphological, immunohistochemical, and molecular (ETV6-NTRK3 translocation) similarities with the mammary secretory carcinoma. The most important differential diagnostic considerations of MASC are low-grade adenocarcinoma not otherwise specified (NOS), cystadenocarcinoma, and acinic cell carcinoma (AciCC). These tumors may share an overlapping morphology with MASC, and additional immunohistochemical studies are required to reinforce the diagnosis. Mammaglobin, GCDFP-15, and p63 staining have been reported in MASC. Our study was designed to check the specificity of these antibodies in MASC compared to other frequent tumors of salivary glands.
  
  
  METHODS
  A series of 62 salivary gland tumors [10 MASCs, 5 adenocarcinomas NOS and 2 cystadenocarcinomas with MASC features and without ETV6 rearrangement, one low-grade cribriform cystadenocarcinoma (LGCCC), 9 AciCCs, 10 MECs, 10 adenoid cystic carcinomas (AdeCCs), 5 polymorphous low-grade adenocarcinomas (PLGAs), and 10 pleomorphic adenomas (PAs)] was analyzed by immunohistochemistry with mammaglobin, GCDFP-15, and p63 antibodies.
  
  
  RESULTS
  Positivity for mammaglobin was observed in all MASCs, cystadenocarcinomas, LGCCC, and PLGAs, in some adenocarcinomas NOS, PAs, and MECs, rarely in AciCCs and never in AdeCCs. Positivity for GCDFP-15 was observed in most of the tumor types except in AdeCCs. Interestingly, cytoplasmic positivity for p63 was observed in most of MASCs and PLGAs while rarely in adenocarcinomas NOS and PAs, and never in the other tumor types.
  
  
  CONCLUSIONS
  Our study revealed the usefulness of mammaglobin and p63 cytoplasmic staining to define which tumors are worth to be screened for ETV6 rearrangement.
authors.affiliation: Department of Pathology, Rangueil University Hospital, Toulouse, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Projetti
authors.forename: Magali
authors.initials: M
authors.lastname: Lacroix-Triki
authors.forename: Elie
authors.initials: E
authors.lastname: Serrano
authors.forename: Sebastien
authors.initials: S
authors.lastname: Vergez
authors.forename: Béatrice Herbault
authors.initials: BH
authors.lastname: Barres
authors.forename: Julie
authors.initials: J
authors.lastname: Meilleroux
authors.forename: Marie-Bernadette
authors.initials: MB
authors.lastname: Delisle
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Uro-Coste
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis*; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D060785:Mammaglobin A / Q000032:analysis*; D000069295:Mammary Analogue Secretory Carcinoma / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D026901:Membrane Transport Proteins; D008875:Middle Aged; D050783:Proto-Oncogene Proteins c-ets / Q000032:analysis; D012097:Repressor Proteins / Q000032:analysis; D012468:Salivary Gland Neoplasms / Q000737:chemistry* / Q000175:diagnosis* / Q000473:pathology; D014157:Transcription Factors / Q000032:analysis*; D025521:Tumor Suppressor Proteins / Q000032:analysis*; D055815:Young Adult; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D006023:Glycoproteins; D060785:Mammaglobin A; D026901:Membrane Transport Proteins; D050783:Proto-Oncogene Proteins c-ets; D012097:Repressor Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D000097764:ETS Translocation Variant 6 Protein; C488892:PIP protein, human; C115051:TP63 protein, human
keywords: gross cystic disease fluid protein 15; immunohistochemistry; mammaglobin; mammary analog secretory carcinoma; p63