title: Hypoglycemic effect of triterpenoid-rich extracts from Euryale ferox shell on normal and streptozotocin-diabetic mice.
abstract: The antioxidant effects of the triterpenoid-rich extracts from Euryale ferox shell (ES) have been confirmed in vitro. This study examined whether the triterpenoid-rich extract from ES eases human hyperglycemia and diabetes caused by metabolic disorders. Normal and streptozocin (STZ)-induced diabetic mice were used as controls for the four groups that received the triterpenoid-rich extracts of ES suspended in distilled water orally at doses of 200, 300, 400, 500±2 mg/L. Body weight, blood glucose and pancreatic tissue morphology were observed after 4 weeks. The expression of protein tyrosine phosphatase-1B (PTP1B) and insulin receptor substrate (IRS-1) proteins, which are related to the regulation of glucose metabolism in vivo, were also investigated. Compared with the model group (LD50 900±2 mg/L), it was found that the triterpenoid-rich extracts of ES could regulate glucose metabolism (P<0.01) and cause body weight to return to normal levels (P<0.05). Islet morphology recovered well, the expression of the negative regulation protein PTP1B gene was reduced and insulin receptor IRS-1 protein expression was increased. These data prove that the triterpenoid-rich extracts from ES have a therapeutic effect on diabetes by insulin resistance.
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China /College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, China.
authors.forename: Huaibo
authors.initials: H
authors.lastname: Yuan
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Meng
authors.affiliation: School of Mathematical Physics and Biology Engineering, Inner Mongolia University of Science and Technology, Baotou, China / College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, China.
authors.forename: Guoze
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China.
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: Gong
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Sun
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: He
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D055504:Insulin Receptor Substrate Proteins / Q000502:physiology; D008297:Male; D051379:Mice; D030702:Nymphaeaceae; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000502:physiology; D013311:Streptozocin; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D007004:Hypoglycemic Agents; D055504:Insulin Receptor Substrate Proteins; C526295:Irs1 protein, mouse; D010936:Plant Extracts; D014315:Triterpenes; D013311:Streptozocin; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; C516609:Ptpn1 protein, mouse