title: Generation of an attenuated Salmonella-delivery strains expressing adhesin and toxin antigens for progressive atrophic rhinitis, and evaluation of its immune responses in a murine model.
abstract: An expression/secretion plasmid containing genes encoding the FimA, CP39, PtfA, ToxA and F1P2 antigens associated with porcine pneumonic pasteurellosis and progressive atrophic rhinitis (PAR) was constructed and harbored in an attenuated Salmonella Typhimurium, which was used as the vaccine candidate. The immune responses induced by this delivery strain were investigated in a murine model. Each antigen secreted from the delivery strain was confirmed by Western blot analysis. Thirty BALB/c mice were divided equally into two groups; group A were intranasally inoculated with the mixture of the five delivery strains, and group B were inoculated with sterile PBS. In group A, all antigen-specific serum IgG were significantly increased compared to those of group B from the 2nd week post-inoculation (WPI) till the 8th WPI. All antigen-specific mucosal IgA in group A were also significantly greater than those of group B. In addition, the significant splenic lymphocyte proliferative responses, the elevations of CD3(+)CD4(+), CD3(+)CD8(+) and B-cell populations, and the induction of IFN-γ expression in group A were observed. In conclusion, the mixture of five delivery strains expressing specific antigen for these diseases was found to be capable of inducing significant humoral and cellular immune responses.
authors.affiliation: Veterinary Public Health, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea.
authors.forename: Hoyeon
authors.initials: H
authors.lastname: Byeon
authors.affiliation: Veterinary Public Health, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Hur
authors.affiliation: Veterinary Public Health, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea.
authors.forename: Bo Ram
authors.initials: BR
authors.lastname: Kim
authors.affiliation: Veterinary Public Health, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea. Electronic address: johnhlee@chonbuk.ac.kr.
authors.forename: John Hwa
authors.initials: JH
authors.lastname: Lee
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D001427:Bacterial Toxins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D016950:Bordetella bronchiseptica; D004195:Disease Models, Animal; D005260:Female; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D008807:Mice, Inbred BALB C; D016979:Pasteurella multocida; D012222:Rhinitis, Atrophic / Q000276:immunology* / Q000517:prevention & control / Q000662:veterinary; D012486:Salmonella typhimurium / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Atrophic rhinitis; Bordetella bronchiseptica; Pasteurella multocida; Pasteurellosis; Salmonella expression and secretion system