title: A "click" chemistry constructed affinity system for 2-oxoglutaric acid receptors and binding proteins.
abstract: An ingenious and specific affinity resin designed to capture the 2-oxoglutaric acid (2-OG) binding proteins was constructed by appending a 2-OG tag to the solid resin via a Cu-catalyzed Huisgen "click" reaction. The so-obtained affinity resin was able to recognize, retain and separate the established 2-OG binding protein NtcA in both the pure form and crude cellular extract, thus constituting a valuable means of searching for novel 2-OG receptors with a view to exploring the signalling pathways of 2-OG, a key Krebs cycle intermediate with unprecedented signalling functions.
authors.affiliation: Aix-Marseille Université and CNRS, Centre Interdisciplinaire de Nanoscience de Marseille, UMR 7325, 13288, Marseille, France. ling.peng@univ-amu.fr.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Assaf
authors.forename: Xinjun
authors.initials: X
authors.lastname: Liu
authors.forename: Fabio
authors.initials: F
authors.lastname: Ziarelli
authors.forename: Amel
authors.initials: A
authors.lastname: Latifi
authors.forename: Otmane
authors.initials: O
authors.lastname: Lamrabet
authors.forename: Gilles
authors.initials: G
authors.lastname: Quéléver
authors.forename: Fanqi
authors.initials: F
authors.lastname: Qu
authors.forename: Cheng-Cai
authors.initials: CC
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Peng
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D057930:Click Chemistry; D007656:Ketoglutaric Acids / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D002352:Carrier Proteins; D007656:Ketoglutaric Acids