title: Purification and antioxidant activities of intracellular zinc polysaccharides from Pleurotus cornucopiae SS-03.
abstract: Intracellular zinc polysaccharides (IZPS) from Pleurotus cornucopiae SS-03 were extracted and purified, and three subfractions (IZPS-1, IZPS-2, and IZPS-3) were separated by DEAE-52 cellulose anion-exchange column chromatography. They showed certain scavenging effects on superoxide anion (O2(*-)) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and positive rising of reducing power in vitro. All the subfractions were found upregulated the enzyme activities of superoxide dismutase (SOD), GSH peroxide (GSH-Px) and catalase (CAT) significantly, decreased the contents of malondialdehyde (MDA) and lipid peroxidation (LPO) remarkably in vivo. In addition, five monosaccharides components, including rhamnose, xylose, mannose, glucose, and galactose with the molar ratio of 1.65:0.05:1:1.50:1.07 and contents of 29.25%, 0.78%, 19.45%, 29.84%, and 20.67%, were investigated by gas chromatography.
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ma
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zheng
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Guoyin
authors.initials: G
authors.lastname: Zhai
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Wang
authors.affiliation: The Second High School of Taian, Taian, Shandong 271000, PR China.
authors.forename: Mengshi
authors.initials: M
authors.lastname: Jia
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: ourlaboratory@163.com.
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010851:Picrates / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D015032:Zinc / Q000032:analysis* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D011134:Polysaccharides; D013481:Superoxides; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D013482:Superoxide Dismutase; D015032:Zinc
keywords: Antioxidant activities; Pleurotus cornucopiae SS-03; Polysaccharides; Purification; Zinc