title: [Highly active fractions of the medicinal leech recombinant destabilase-lysozyme].
abstract: From the highly purified but lowly active recombinant protein Destabilas-Lysozyme (Dest-Lys) by use cation-exchange column TSK CM 3-SW chromatography, it was separated non-active fraction IV, contained 90% of protein. Fractions I, II and III, represented proteins with lysozyme and isopeptidase activities. Their lysozyme activity correlates with the activity of natural Des-Lys. The ratio of the activities in fractions I - III is such, that maximal lysozyme activity is concentrated in fraction III, isopeptidase - in fraction I. It is discussed the possibility of Dest-Lys different functions regulation is depended on the formation of protein complex forms.
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Fadeeva
authors.forename: N V
authors.initials: NV
authors.lastname: Antipova
authors.forename: I P
authors.initials: IP
authors.lastname: Baskova
authors.forename: L L
authors.initials: LL
authors.lastname: Zavalova
mesh_terms: D000818:Animals; D019759:Carbon-Nitrogen Lyases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D010450:Endopeptidases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D047709:Hirudo medicinalis / Q000737:chemistry* / Q000201:enzymology; D007700:Kinetics; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D005343:Fibrinolytic Agents; D011994:Recombinant Proteins; D009113:Muramidase; D010450:Endopeptidases; C045348:fibrin destabilase; D019759:Carbon-Nitrogen Lyases; C034300:isopeptidase
keywords: destabilase-lysozyme of Hirudo medicinalis; forms of recombinant enzyme; lysozyme and isopeptidase functions