title: [Glybenclamide regulate ERK1/2 signal pathway during hypoxia hypercapnia pulmonary vasoconstriction in rats].
abstract: OBJECTIVE
  To investigate the role and significance of ATP-sensitive K+ channels in the pathological process of hypoxia hypercapnia-induced pulmonary vasoconstriction (HHPV) and the relationship with ERK1/2 signal pathway in rats.
  
  
  METHODS
  We made the third pulmonary artery rings of SD rats, used the model of pulmonary artery rings perfusion in vitro. Under acute hypoxia hypercapnia condition, and observed the effects of the three stages of HHPV incubated by glybenclamide(Gly) and the combined application of Gly and U0126. At the same time, the values of rings' tension changes were recorded via the method of hypoxia hypercapnia conditions reactivity.
  
  
  RESULTS
  Under the normoxia condition, the values of the third pulmonary artery rings tension were relatively stable, but under the hypoxia hypercapnia condition, we observed a biphasic pulmonary artery contractile response compared with N group (P < 0.05, P < 0.01). When the third pulmonary artery rings incubated by Gly, it's phase II persistent vasoconstriction was enhanced compared with the H group (P < 0.05, P < 0.01), and the phase I vasoconstriction was also heightened. Moreover, under the hypoxia hypercapnia condition, U0126 could significantly relieve the phase II persistent vasoconstriction compared with HD group (P < 0.05, P < 0.01) induced by Gly, but the phase I acute vasoconstriction and the phase I vasodilation had no changes (P > 0.05).
  
  
  CONCLUSIONS
  Gly may mediate HHPV via activating ERK1/2 signal transduction pathway.
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Ma
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Wang
authors.forename: Hai-E
authors.initials: HE
authors.lastname: Chen
authors.forename: Lin-Jing
authors.initials: LJ
authors.lastname: Huang
authors.forename: Jin-Bo
authors.initials: JB
authors.lastname: He
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Wan-Tie
authors.initials: WT
authors.lastname: Wang
mesh_terms: D000818:Animals; D005905:Glyburide / Q000494:pharmacology*; D006935:Hypercapnia / Q000378:metabolism / Q000503:physiopathology; D000860:Hypoxia / Q000378:metabolism / Q000503:physiopathology*; D066298:In Vitro Techniques; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D011651:Pulmonary Artery / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: D005905:Glyburide