title: Enhanced electrocatalytic oxidation of isoniazid at electrochemically modified rhodium electrode for biological and pharmaceutical analysis.
abstract: A simple and sensitive electrochemical method has been proposed for the determination of isoniazid (INZ). For the first time, rhodium (Rh) modified glassy carbon electrode (GCE) has been employed for the determination of INZ by linear sweep voltammetry technique (LSV). Compared with the unmodified electrode, the proposed Rh modified electrode provides strong electrocatalytic activity toward INZ with significant enhancement in the anodic peak current. Scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) results reveal the morphology of Rh particles. With the advantages of wide linearity (70-1300μM), good sensitivity (0.139μAμM(-1)cm(-2)) and low detection limit (13μM), this proposed sensor holds great potential for the determination of INZ in real samples. The practicality of the proposed electrode for the detection of INZ in human urine and blood plasma samples has been successfully demonstrated using LSV technique. Through the determination of INZ in commercially available pharmaceutical tablets, the practical applicability of the proposed method has been validated. The recovery results are found to be in good agreement with the labeled amounts of INZ in tablets, thus showing its great potential for use in clinical and pharmaceutical analysis.
authors.affiliation: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Cheemalapati
authors.affiliation: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC. Electronic address: smchen78@ms15.hinet.net.
authors.forename: Shen-Ming
authors.initials: SM
authors.lastname: Chen
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: M Ajmal
authors.initials: MA
authors.lastname: Ali
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Fahad M A
authors.initials: FM
authors.lastname: Al-Hemaid
mesh_terms: D000328:Adult; D002244:Carbon / Q000737:chemistry; D002384:Catalysis; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007538:Isoniazid / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012238:Rhodium / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D002244:Carbon; D012238:Rhodium; D007538:Isoniazid
keywords: Biological sample; Isoniazid; Plasma; Rhodium; Urine