title: Using pooled miR30-shRNA library for cancer lethal and synthetic lethal screens.
abstract: Pooled shRNA library is a powerful, rapid, and cost-effective technology to carry out functional genomic screens in mammalian cells. This approach has been applied extensively to identify genetic dependencies in cancer cells that might be exploited for therapeutic purposes. In this chapter we provide a detailed protocol for using the Hannon-Elledge miR30-based library to conduct dropout screens in cancer cell lines. This protocol is readily adaptable to other pooled shRNA libraries and should facilitate the functional annotation of the human genome.
authors.affiliation: Medical Oncology Branch, Center For Cancer Research, National Cancer Institute, Building 10, Magnuson CC, Room 3B43, Bethesda, MD, 20892, USA.
authors.forename: Liam Changwoo
authors.initials: LC
authors.lastname: Lee
authors.forename: Shaojian
authors.initials: S
authors.lastname: Gao
authors.forename: Qiuning
authors.initials: Q
authors.lastname: Li
authors.forename: Ji
authors.initials: J
authors.lastname: Luo
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D015723:Gene Library; D005804:Genes, Lethal; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation; D009369:Neoplasms / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D034741:RNA, Small Interfering