title: Fast and accurate identification of dermatophytes by matrix-assisted laser desorption ionization-time of flight mass spectrometry: validation in the clinical laboratory.
abstract: The performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.
authors.affiliation: Scientific Institute of Public Health, Service of Mycology and Aerobiology, Brussels, Belgium ann.packeu@wiv-isp.be.
authors.forename: A
authors.initials: A
authors.lastname: Packeu
authors.affiliation: Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: De Bel
authors.affiliation: Aix Marseille Université, IP-TPT UMR MD3, Marseille, France Parasitology and Mycology Laboratory, CHU Timone-adultes, APHM, Marseille, France.
authors.forename: C
authors.initials: C
authors.lastname: l'Ollivier
authors.affiliation: Aix Marseille Université, IP-TPT UMR MD3, Marseille, France Parasitology and Mycology Laboratory, CHU Timone-adultes, APHM, Marseille, France.
authors.forename: S
authors.initials: S
authors.lastname: Ranque
authors.affiliation: Scientific Institute of Public Health, Service of Mycology and Aerobiology, Brussels, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Detandt
authors.affiliation: Scientific Institute of Public Health, Service of Mycology and Aerobiology, Brussels, Belgium BCCM/IHEM Fungal Collection, Scientific Institute of Public Health, Mycology and Aerobiology Section, Brussels, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Hendrickx
mesh_terms: D003883:Arthrodermataceae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003881:Dermatomycoses / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D013048:Specimen Handling / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors