title: Synthetic biodegradable hydrogel delivery of demineralized bone matrix for bone augmentation in a rat model.
abstract: There exists a strong clinical need for a more capable and robust method to achieve bone augmentation, and a system with fine-tuned delivery of demineralized bone matrix (DBM) has the potential to meet that need. As such, the objective of the present study was to investigate a synthetic biodegradable hydrogel for the delivery of DBM for bone augmentation in a rat model. Oligo(poly(ethylene glycol) fumarate) (OPF) constructs were designed and fabricated by varying the content of rat-derived DBM particles (either 1:3, 1:1 or 3:1 DBM:OPF weight ratio on a dry basis) and using two DBM particle size ranges (50-150 or 150-250 μm). The physical properties of the constructs and the bioactivity of the DBM were evaluated. Selected formulations (1:1 and 3:1 with 50-150 μm DBM) were evaluated in vivo compared to an empty control to investigate the effect of DBM dose and construct properties on bone augmentation. Overall, 3:1 constructs with higher DBM content achieved the greatest volume of bone augmentation, exceeding 1:1 constructs and empty implants by 3- and 5-fold, respectively. As such, we have established that a synthetic, biodegradable hydrogel can function as a carrier for DBM, and that the volume of bone augmentation achieved by the constructs correlates directly to the DBM dose.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77251, USA.
authors.forename: Lucas A
authors.initials: LA
authors.lastname: Kinard
authors.affiliation: Department of Bioengineering, Rice University, Houston, TX 77251, USA.
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Dahlin
authors.affiliation: Department of Bioengineering, Rice University, Houston, TX 77251, USA.
authors.forename: Johnny
authors.initials: J
authors.lastname: Lam
authors.affiliation: Department of Bioengineering, Rice University, Houston, TX 77251, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Bioengineering, Rice University, Houston, TX 77251, USA.
authors.forename: Esther J
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Bioengineering, Rice University, Houston, TX 77251, USA.
authors.forename: F Kurtis
authors.initials: FK
authors.lastname: Kasper
authors.affiliation: Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77251, USA; Department of Bioengineering, Rice University, Houston, TX 77251, USA. Electronic address: mikos@rice.edu.
authors.forename: Antonios G
authors.initials: AG
authors.lastname: Mikos
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001857:Bone Matrix / Q000737:chemistry*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects; D019245:Compressive Strength / Q000187:drug effects; D004195:Disease Models, Animal; D005650:Fumarates / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D008297:Male; D008422:Materials Testing; D051379:Mice; D019637:Orthopedic Procedures / Q000379:methods*; D010012:Osteogenesis / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry; D011916:Rats, Inbred F344; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D005650:Fumarates; C508734:poly(ethylene glycol fumarate); D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D000469:Alkaline Phosphatase
keywords: Bone augmentation; Demineralized bone matrix; Oligo(poly(ethylene glycol) fumarate); Synthetic hydrogel