title: Dietary manganese supplementation influences the expression of transporters involved in iron metabolism in chickens.
abstract: To investigate the effects of dietary manganese (Mn) supplementation on iron (Fe) metabolism, a total of 480 50-week-old hens were fed the basal diet (control, 24.35 mg Mn/kg) without Mn supplementation for 6 weeks to reduce Mn storage in the body. Hens were then randomly assigned to one of three treatments, which included the control and control added with 60 or 300 mg Mn/kg diet (M-Mn or H-Mn). Duodenum, heart, liver, and tibia were collected in hens after 12-week feeding period. No significant differences were observed in egg production, feed/egg ratio, shell breaking strength, and shell thickness among different treatments. Compared with control or M-Mn, H-Mn decreased (P < 0.05) serum Fe concentration, while increased (P < 0.05) total Fe-binding capacity (TIBC). The Fe concentration decreased (P < 0.05) in duodenum, and tended to reduce (P < 0.10) in liver from control to M-Mn and to H-Mn; whereas, dietary Mn supplementation did not influence (P > 0.10) Fe concentration in the heart and tibia. In conjunction with reduced Fe retention, DMT1 mRNA expression decreased (P < 0.05) with dietary Mn concentration increasing in the duodenum and liver. Duodenal FPN1 mRNA level was higher (P < 0.05) in H-Mn group than that in control or M-Mn group, while hepatic FPN1 mRNA expression was lower (P < 0.05) in M-Mn or H-Mn group when compared with control. The results demonstrated that dietary Mn supplementation decreased Fe concentration in duodenum and liver of hens, which may be related to the alteration of DMT1 and FPN1 expression in these tissues.
authors.affiliation: Institute of Animal Nutrition, Feed Engineering Research Centre of Sichuan Province, Sichuan Agricultural University, Xinkang Road 46#, Yucheng District, 625014, Ya'an, Sichuan, People's Republic of China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Bai
authors.forename: Lirong
authors.initials: L
authors.lastname: Huang
authors.forename: Yuheng
authors.initials: Y
authors.lastname: Luo
authors.forename: Leilei
authors.initials: L
authors.lastname: Wang
authors.forename: Xuemei
authors.initials: X
authors.lastname: Ding
authors.forename: Jianping
authors.initials: J
authors.lastname: Wang
authors.forename: Qiufeng
authors.initials: Q
authors.lastname: Zeng
authors.forename: Keying
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins / Q000096:biosynthesis*; D002645:Chickens; D019587:Dietary Supplements; D005786:Gene Expression Regulation / Q000187:drug effects*; D007501:Iron / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology*; D009928:Organ Specificity / Q000187:drug effects; D000098742:Ferroportin; D000099236:Solute Carrier Family 11, Member 2
chemical_list: D027682:Cation Transport Proteins; D007501:Iron; D008345:Manganese; D000098742:Ferroportin; D000099236:Solute Carrier Family 11, Member 2