title: Thermo-sensitive liposomes loaded with doxorubicin and lysine modified single-walled carbon nanotubes as tumor-targeting drug delivery system.
abstract: This report focuses on the thermo-sensitive liposomes loaded with doxorubicin and lysine-modified single-walled carbon nanotube drug delivery system, which was designed to enhance the anti-tumor effect and reduce the side effects of doxorubicin. Doxorubicin-lysine/single-walled carbon nanotube-thermo-sensitive liposomes was prepared by reverse-phase evaporation method, the mean particle size was 232.0 ± 5.6 nm, and drug entrapment efficiency was 86.5 ± 3.7%. The drug release test showed that doxorubicin released more quickly at 42℃ than at 37℃. Compared with free doxorubicin, doxorubicin-lysine/single-walled carbon nanotube-thermo-sensitive liposomes could efficiently cross the cell membranes and afford higher anti-tumor efficacy on the human hepatic carcinoma cell line (SMMC-7721) cells in vitro. For in vivo experiments, the relative tumor volumes of the sarcomaia 180-bearing mice in thermo-sensitive liposomes group and doxorubicin group were significantly smaller than those of N.S. group. Meanwhile, the combination of near-infrared laser irradiation at 808 nm significantly enhanced the tumor growth inhibition both on SMMC-7721 cells and the sarcomaia 180-bearing mice. The quality of life such as body weight, mental state, food and water intake of sarcomaia 180 tumor-bearing mice treated with doxorubicin-lysine/single-walled carbon nanotube-thermo-sensitive liposomes were much higher than those treated with doxorubicin. In conclusion, doxorubicin-lysine/single-walled carbon nanotube-thermo-sensitive liposomes combined with near-infrared laser irradiation at 808 nm may potentially provide viable clinical strategies for targeting delivery of anti-cancer drugs.
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China Pharmacy College, Henan University of TCM, Zhengzhou, PR China.
authors.forename: Xiali
authors.initials: X
authors.lastname: Zhu
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China.
authors.forename: Yingxia
authors.initials: Y
authors.lastname: Xie
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China.
authors.forename: Heqing
authors.initials: H
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Shi
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China zhangzz08@126.com.
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001835:Body Weight; D002244:Carbon / Q000737:chemistry; D002453:Cell Cycle; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000737:chemistry*; D004337:Drug Carriers; D005260:Female; D006801:Humans; D007834:Lasers; D008081:Liposomes / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009368:Neoplasm Transplantation; D010316:Particle Size; D010778:Photochemotherapy / Q000379:methods; D011788:Quality of Life; D013696:Temperature
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D037742:Nanotubes, Carbon; D002244:Carbon; D004317:Doxorubicin; D008239:Lysine
keywords: Single-walled carbon nanotubes; doxorubicin; near-infrared radiation; thermo-sensitive liposomes; tumor-targeting