title: Cimetidine effects on the immunosuppression induced by burn injury.
abstract: Although many studies on the immune response following burn injuries have been reported, more attention has been given to the immunosuppression mechanism and mediators that shape the process of immune suppression. Specifically, information is not available concerning the immunomodulatory effects of the drugs which are involved in the immune response restoration. In this study, we investigated the effects of Cimetidine on the modulation of immune response in patients with burn injury of 20-60%. Two groups of patients were involved in this study; the patients in one group were treated with 15 mg/kg per day of Cimetidine while the patients in the other group were treated with placebo. Peripheral blood mononuclear cell (PBMC) expressing CD3, CD4, CD8, CD19 and CD3/HLA-DR was analyzed by flow cytometry. Cell proliferation assay using H3 thymidine was performed on PBMC samples. The proliferation assay showed a significant suppression of cell proliferation rate in post-burn patients (p = 0.001). We observed a significant reduction in the lymphocyte count (p = 0.001) and frequency of CD3 (p = 0.007) and CD4 (p = 0.001) T cells in post-burn patients. Also, the frequency of CD 19+ and HLA DR+ cells was increased compare to normal donors following burn injury. Treatment with Cimetidine increased the frequency of CD8+ T cells in the patient's peripheral blood. The PBMC proliferation rate was restored following the treatment with Cimetidine (p = 0.02). Our data indicates that Cimetidine may have beneficial effects on cell mediated immunity following burn injury.
authors.affiliation: Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran; Immune Gene therapy Lab, CCK, Karolinska University Hospital Solna, Stockholm, Sweden. Electronic address: parviz.kokhaei@ki.se.
authors.forename: Parviz
authors.initials: P
authors.lastname: Kokhaei
authors.affiliation: Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran; Student's Research Committee, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Mahdieh Shokrollahi
authors.initials: MS
authors.lastname: Barough
authors.affiliation: Department of Immunology, Medical School, Tarbiat Modarres University, Tehran, Iran.
authors.forename: Zuhair M
authors.initials: ZM
authors.lastname: Hassan
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D002056:Burns / Q000188:drug therapy* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002927:Cimetidine / Q000008:administration & dosage* / Q000009:adverse effects; D006684:HLA-DR Antigens / Q000378:metabolism; D006635:Histamine H2 Antagonists / Q000008:administration & dosage* / Q000009:adverse effects; D006706:Homeostasis / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D016130:Immunophenotyping; D007165:Immunosuppression Therapy; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D018655:Lymphocyte Count
chemical_list: D015703:Antigens, CD; D006684:HLA-DR Antigens; D006635:Histamine H2 Antagonists; D002927:Cimetidine
keywords: B lymphocyte; Burn injury; Cimetidine; T lymphocyte