title: Mulberry leaf phenolics ameliorate hyperglycemia-induced oxidative stress and stabilize mitochondrial membrane potential in HepG2 cells.
abstract: To investigate the effect of phenolics in mulberry leaves (mulberry leaf phenolics; MLP) on hyperglycemia-induced oxidative stress and mitochondrial membrane potential (ΔΨm) in HepG2 cells; we treated HepG2 with glucose [5.5 (N-Glc) or 50 mmol/L (Hi-Glc)] with or without MLP at 10 or 100 µmol/L gallic acid equivalents and assessed level of reactive oxidant species (ROS), ΔΨm, malondialdehyde (MDA) and nuclear factor-kappaB (NF-κB) activation. Hi-Glc-induced oxidative damage was demonstrated by a series of increase in superoxides (560%, 0.5 h), MDA (400%, 24 h), NF-κB activation (474%, 4 h) and a wild fluctuation of ΔΨm relative to the control cells (p ≤ 0.05). MLP treatments ameliorate Hi-Glc-induced negative effects by a 40% reduction in ROS production, 34-44% reduction in MDA production, over 35% inhibition of NF-κB activation, as well as exert protective effect on HepG2 cells from change in ΔΨm. Our data show that MLP in vitro can protect hepatoctyes from hyperglycemia-induced oxidative damages.
authors.affiliation: Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences , Guangzhou , PR China and.
authors.forename: Yu-Xiao
authors.initials: YX
authors.lastname: Zou
authors.forename: Wei-Zhi
authors.initials: WZ
authors.lastname: Shen
authors.forename: Sen-Tai
authors.initials: ST
authors.lastname: Liao
authors.forename: Fan
authors.initials: F
authors.lastname: Liu
authors.forename: Shan-Qing
authors.initials: SQ
authors.lastname: Zheng
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Blumberg
authors.forename: C-Y Oliver
authors.initials: CY
authors.lastname: Chen
mesh_terms: D005947:Glucose / Q000008:administration & dosage / Q000009:adverse effects / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D006943:Hyperglycemia / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D031625:Morus / Q000737:chemistry*; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D013481:Superoxides / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D010636:Phenols; D010936:Plant Extracts; D013481:Superoxides; D008315:Malondialdehyde; D005947:Glucose
keywords: HepG2; hyperglycemia; mitochondria; mulberry leaves; oxidative stress; phenolics