title: Effect of thermal processing on estimated metabolizable protein supply to dairy cattle from camelina seeds: relationship with protein molecular structural changes.
abstract: This study evaluated the effect of thermal processing on the estimated metabolizable protein (MP) supply to dairy cattle from camelina seeds (Camelina sativa L. Crantz) and determined the relationship between heat-induced changes in protein molecular structural characteristics and the MP supply. Seeds from two camelina varieties were sampled in two consecutive years and were either kept raw or were heated in an autoclave (moist heating) or in an air-draft oven (dry heating) at 120 °C for 1 h. The MP supply to dairy cattle was modeled by three commonly used protein evaluation systems. The protein molecular structures were analyzed by Fourier transform/infrared-attenuated total reflectance molecular spectroscopy. The results showed that both the dry and moist heating increased the contents of truly absorbable rumen-undegraded protein (ARUP) and total MP and decreased the degraded protein balance (DPB). However, the moist-heated camelina seeds had a significantly higher (P < 0.05) content of ARUP and total MP and a significantly lower (P < 0.05) content of DPB than did the dry-heated camelina seeds. The regression equations showed that intensities of the protein molecular structural bands can be used to estimate the contents of ARUP, MP, and DPB with high accuracy (R(2) > 0.70). These results show that protein molecular structural characteristics can be used to rapidly assess the MP supply to dairy cattle from raw and heat-treated camelina seeds.
authors.affiliation: Department of Animal and Poultry Science, University of Saskatchewan , Saskatoon, SK S7N 5A8, Canada.
authors.forename: Quanhui
authors.initials: Q
authors.lastname: Peng
authors.forename: Nazir A
authors.initials: NA
authors.lastname: Khan
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Xuewei
authors.initials: X
authors.lastname: Zhang
authors.forename: Peiqiang
authors.initials: P
authors.lastname: Yu
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D019607:Brassicaceae / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D003296:Cooking / Q000379:methods*; D006358:Hot Temperature; D009753:Nutritive Value; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010940:Plant Proteins
keywords: camelina seed; protein evaluation system; protein metabolic characteristics; protein molecular structure