title: Heavy oils, principally long-chain n-alkanes secreted by Aureobasidium pullulans var. melanogenum strain P5 isolated from mangrove system.
abstract: In this study, the yeast strain P5 isolated from a mangrove system was identified to be a strain of Aureobasidium pullulans var. melanogenum and was found to be able to secrete a large amount of heavy oil into medium. After optimization of the medium for heavy oil production and cell growth by the yeast strain P5, it was found that 120.0 g/l of glucose and 0.1 % corn steep liquor were the most suitable for heavy oil production. During 10-l fermentation, the yeast strain P5 produced 32.5 g/l of heavy oil and cell mass was 23.0 g/l within 168 h. The secreted heavy oils contained 66.15 % of the long-chain n-alkanes and 26.4 % of the fatty acids, whereas the compositions of the fatty acids in the yeast cells were only C16:0 (21.2 %), C16:1(2.8 %), C18:0 (2.9 %), C18:1 (39.8 %), and C18:2 (33.3 %). We think that the secreted heavy oils may be used as a new source of petroleum in marine environments. This is the first report of yeast cells which can secrete the long-chain n-alkanes.
authors.affiliation: UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, 266003, Qingdao, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chi
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Wang
authors.forename: Guang-Lei
authors.initials: GL
authors.lastname: Liu
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Chi
mesh_terms: D000473:Alkanes / Q000737:chemistry / Q000378:metabolism*; D001203:Ascomycota / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D008969:Molecular Sequence Data; D010578:Petroleum / Q000032:analysis / Q000378:metabolism*; D010802:Phylogeny; D010944:Plants / Q000382:microbiology*; D053833:Wetlands
chemical_list: D000473:Alkanes; D005227:Fatty Acids; D010578:Petroleum