title: Pueraria mirifica leaves, an alternative potential isoflavonoid source.
abstract: We investigated the major leaf isoflavonoid contents of Pueraria mirifica from three different cultivars (PM-III, PM-IV, and PM-V) using reverse RP-HPLC analysis. The proportions and net levels of puerarin, daidzin, genistin, and daidzein in P. mirifica leaves were found to depend on the plant cultivar and to correlate with cultivation temperature and rainfall amount. The crude leaf-extracts were tested using the Yeast Estrogen Screen (YES) assay with both human estrogen receptors (hERα and hERβ). Their estrogenic activity was higher when determined by the YES system containing hERβ than that with hERα and was also higher when the Δsnq2 than the wildtype yeast was employed. The results open the possibility of selecting and cultivating certain P. mirifica cultivars at a farm scale to produce a sufficient supply of leaf material to act as a starting source for the commercial scale extraction of these major isoflavonoids.
authors.affiliation: a Faculty of Science, Program in Biotechnology , Chulalongkorn University , Bangkok , Thailand.
authors.forename: Jutarmas
authors.initials: J
authors.lastname: Jungsukcharoen
authors.forename: Binar Asrining
authors.initials: BA
authors.lastname: Dhiani
authors.forename: Wichai
authors.initials: W
authors.lastname: Cherdshewasart
authors.forename: Nawaporn
authors.initials: N
authors.lastname: Vinayavekhin
authors.forename: Polkit
authors.initials: P
authors.lastname: Sangvanich
authors.forename: Chuenchit
authors.initials: C
authors.lastname: Boonchird
mesh_terms: D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D047628:Estrogen Receptor alpha / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D018515:Plant Leaves / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry* / Q000254:growth & development
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D005419:Flavonoids
keywords: Pueraria mirifica; YES assay; estrogenic activity; isoflavonoid