title: Bioactive constituents of Cirsium japonicum var. australe.
abstract: Cirsium japonicum var. australe, used as a folk medicine in Taiwan, has been employed traditionally in the treatment of diabetes and inflammatory symptoms. Bioactivity-guided fractionation of its ethanolic extract, utilizing centrifugal partition chromatography monitored by DPPH-TLC analysis, led to the isolation of three new acetylenic phenylacrylic acid esters (1-3) and two new polyacetylenes (4 and 5), together with seven known compounds (6-12). The structures of 1-5 were elucidated by spectroscopic methods including 1D and 2D NMR techniques. The absolute configurations of 4 and 7 were determined utilizing Mosher's method and ECD/CD experiments. The DPPH scavenging activity of the constituents isolated from the C. japonicum var. australe ethanolic extract was evaluated. The potential antidiabetic activity of some of the isolates was evaluated using in vitro cellular glucose uptake and oil red staining assays.
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University , Kaohsiung 80708, Taiwan.
authors.forename: Wan-Chun
authors.initials: WC
authors.lastname: Lai
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.forename: Balázs
authors.initials: B
authors.lastname: Dankó
authors.forename: Yuan-Bin
authors.initials: YB
authors.lastname: Cheng
authors.forename: Tusty-Jiuan
authors.initials: TJ
authors.lastname: Hsieh
authors.forename: Chi-Ting
authors.initials: CT
authors.lastname: Hsieh
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Tsai
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Shazly
authors.forename: Ana
authors.initials: A
authors.lastname: Martins
authors.forename: Judit
authors.initials: J
authors.lastname: Hohmann
authors.forename: Attila
authors.initials: A
authors.lastname: Hunyadi
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
mesh_terms: D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D001391:Azo Compounds; D001713:Biphenyl Compounds / Q000494:pharmacology; D031180:Cirsium / Q000737:chemistry*; D003920:Diabetes Mellitus / Q000188:drug therapy; D005947:Glucose / Q000378:metabolism; D008519:Medicine, Traditional; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010851:Picrates / Q000494:pharmacology; D053279:Polyynes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013624:Taiwan
chemical_list: D000893:Anti-Inflammatory Agents; D001391:Azo Compounds; D001713:Biphenyl Compounds; D010851:Picrates; D053279:Polyynes; C004931:1,1-diphenyl-2-picrylhydrazyl; C011049:oil red O; D005947:Glucose