title: Overexpression of β3 Chains of Laminin-332 is Associated With Clinicopathologic Features and Decreased Survival in Patients With Pancreatic Adenocarcinoma.
abstract: BACKGROUND
  Laminin-332 (LM-332, formerly termed laminin-5) is a heterotrimeric glycoprotein that promotes cellular adhesion and migration. The heterotrimer consists of an α3, a β3, and a γ2 chain. The aim of this investigation was to clarify the clinicopathologic significance of laminin-332β3 (LNβ3) chain expression and determine its influence on survival in pancreatic ductal adenocarcinoma.
  
  
  METHODS
  Quantitative real-time polymerase chain reaction was used to validate and detect the expression of LNβ3 mRNA in 37 pancreatic carcinoma tissue specimens and non-neoplastic pancreatic tissue samples. In addition, the protein expression of LNβ3 was detected by immunohistochemistry methods in 96 pancreatic carcinoma specimens and 90 non-neoplastic pancreatic tissues. We analyzed the association between immunohistochemically detected LNβ3 expression in pancreatic ductal adenocarcinoma and clinicopathologic characteristics. Survival curves were completed using the Kaplan-Meier method and compared using log-rank analysis.
  
  
  RESULTS
  Quantitative real-time polymerase chain reaction indicated that the relative value of LNβ3 mRNA was 1.427±1.554 and 1.423±1.439 by 2 in pancreatic carcinoma and non-neoplastic pancreatic tissues, respectively, values that were not statistically associated (P=0.991). Immunostaining for LNβ3 was expressed in all patients with pancreatic ductal adenocarcinoma. LNβ3 expression was related to differentiation (P=0.000) and advanced stage (P=0.034). Tumors with low expression of LNβ3 had a survival advantage compared with tumors that had high expression of LNβ3 (P=0.016). Multivariate analysis indicated that location is an independent predictor of overall survival, whereas other clinicopathologic characteristics such as tumor size, duodenal invasion, differentiation, extent of invasion, hepatic metastasis, and expression of LNβ3 were not.
  
  
  CONCLUSIONS
  Our results suggest that LNβ3 expression may play a key role in the progression and prognosis of pancreatic ductal adenocarcinoma.
authors.affiliation: *Department of Surgery, Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University †Key Laboratory of Combined Multiorgan Transplantation, Ministry of Public Health Key Laboratory of Organ Transplantation, Hangzhou ‡Department of Surgery, Jinhua Municipal Central Hospital, Jinhua, Zhejiang Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Weilin
authors.initials: W
authors.lastname: Wang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wei
authors.forename: Dongkai
authors.initials: D
authors.lastname: Zhou
authors.forename: Xinyi
authors.initials: X
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.forename: Pengfei
authors.initials: P
authors.lastname: Huang
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D015996:Survival Rate; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D009363:Neoplasm Proteins; D000097794:Kalinin