title: Ultrasensitive aptamer based detection of β-conglutin food allergen.
abstract: Lupine has been increasingly used in food applications due to its high nutritional value and excellent functional properties. However, there has been a response to the increasing number of severe cases of lupine allergies reported during the last decade, and as a result lupine was recently added to the list of substances requiring mandatory advisory labelling on foodstuffs sold in the European Union. In this paper we report the robust and ultrasensitive detection of the anaphylactic β-conglutin allergen using Apta-PCR achieving a detection limit of 85 pM (25 ng mL(-1)). No cross-reactivity with other conglutins or plant species potentially used in lupine containing foodstuffs was observed. This robust method provides an effective analytical tool for the detection and quantification of the toxic β-conglutin subunit present in lupine flour.
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Department of Chemical Engineering, Universitat Rovira i Virgili, 43007 Tarragona, Spain. Electronic address: marketa.svobodova@urv.cat.
authors.forename: Marketa
authors.initials: M
authors.lastname: Svobodova
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Department of Chemical Engineering, Universitat Rovira i Virgili, 43007 Tarragona, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Mairal
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Department of Chemical Engineering, Universitat Rovira i Virgili, 43007 Tarragona, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Nadal
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Department of Chemical Engineering, Universitat Rovira i Virgili, 43007 Tarragona, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Bermudo
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Department of Chemical Engineering, Universitat Rovira i Virgili, 43007 Tarragona, Spain; Institució Catalana de Recerca I Estudis Avancats, Passeig Lluís Companys 23, 08010 Barcelona, Spain. Electronic address: ckosulli@etse.urv.es.
authors.forename: Ciara K
authors.initials: CK
authors.lastname: O'Sullivan
mesh_terms: D000485:Allergens / Q000276:immunology*; D052157:Aptamers, Nucleotide; D005512:Food Hypersensitivity / Q000276:immunology*; D031297:Lupinus / Q000737:chemistry*; D055314:Seed Storage Proteins / Q000737:chemistry / Q000276:immunology*
chemical_list: D000485:Allergens; D052157:Aptamers, Nucleotide; D055314:Seed Storage Proteins; C042457:conglutin protein, Lupinus angustifolius
keywords: Apta-PCR; Lupine; β-Conglutin allergen; β-Conglutin binding aptamer (β-CBA)