title: Gastroprotective effect of taurine zinc solid dispersions against absolute ethanol-induced gastric lesions is mediated by enhancement of antioxidant activity and endogenous PGE2 production and attenuation of NO production.
abstract: Zinc plays a key role in maintaining gastric mucosal integrity, while alcohol dependency can lead to low zinc status. Complexes containing zinc have been reported to have better ability to protect gastric mucosa than the compounds alone. In this study, taurine zinc [Zn(NH3CH2CH2SO3)2] solid dispersions (SDs) were synthesized and investigated in an ethanol-induced ulcer model in rats. Gastric ulcer index; gastric mucosa malondialdehyde (MDA) level, glutathione (GSH) content, superoxide dismutase (SOD) activity and prostaglandin E2 (PGE2) production; and serum nitric oxide (NO) were assessed and histological analysis of the gastric mucosa tissue was performed. Taurine zinc (100, 200 mg/kg) SDs protected rat gastric mucosa from ethanol-induced injury. Moreover, the gastroprotective effect of taurine zinc SDs was accompanied by a decrease in serum NO and significant increase in gastric prostaglandin E2 (PGE2). When indomethacin, a non-selective COX inhibitor was administered before the last dose of taurine zinc, the gastroprotective effect of taurine zinc was weakened. Furthermore, taurine zinc (200 mg/kg) SDs protected against ulceration more significantly than the same dose of taurine alone, suggesting a synergistic effect between taurine and zinc. These results indicate taurine zinc protects the gastric mucosa against ethanol-induced damage by elevating antioxidants, decreasing lipid peroxidation and inhibiting the production of nitric oxide. The gastroprotective effect of taurine zinc was also partially mediated by endogenous PGE2 production.
authors.affiliation: Lab of Traditional Chinese Medicine and Marine Drugs, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong Province, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Yu
authors.affiliation: Lab of Traditional Chinese Medicine and Marine Drugs, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong Province, China.
authors.forename: Xue-Ting
authors.initials: XT
authors.lastname: Mei
authors.affiliation: Lab of Traditional Chinese Medicine and Marine Drugs, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong Province, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Zheng
authors.affiliation: Lab of Traditional Chinese Medicine and Marine Drugs, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong Province, China. Electronic address: Donghuixu007@163.com.
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Xu
mesh_terms: D000818:Animals; D015232:Dinoprostone / Q000378:metabolism; D000431:Ethanol; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000097:blood; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology* / Q000627:therapeutic use*
chemical_list: D020011:Protective Agents; D013654:Taurine; D009569:Nitric Oxide; D000431:Ethanol; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D015232:Dinoprostone
keywords: Antioxidant; Antiulcer; Indomethacin; NO; PGE(2); Taurine zinc