title: Investigation of antiarthritic potential of Plumeria alba L. leaves in acute and chronic models of arthritis.
abstract: OBJECTIVE
  The present investigation was designed to evaluate antiarthritic potential of fractions of hydroalcoholic extract from leaves of P. alba.
  
  
  METHODS
  Plumeria alba L. leaves were extracted with hydroalcohol (30 : 70) to obtain hydroalcoholic extract of P. alba. This extract was further fractionated with solvents ethyl acetate and n-butanol to obtain EAPA and BPA, respectively. These fractions were tested against formaldehyde and Freund's complete adjuvant (FCA) induced arthritis. Arthritis assessment, paw volume, body weight, motor incoordination, and nociceptive threshold were measured. On day 21, the animals were sacrificed and histopathology was done.
  
  
  RESULTS
  The 100 and 200 mg/kg doses of EAPA and BPA caused a significant (P ≤ 0.05-0.01) reduction in paw swelling in both models. Erythrocyte sedimentation rate (ESR) and spleen weight decreased significantly (P < 0.01) in arthritic rats treated with extracts. There was significant (P < 0.05) improvement in thymus weight in EAPA treated rats whereas significant (P < 0.01) improvement was also seen in haemoglobin level (Hb) in diclofenac treated group. Motor incoordination and nociceptive threshold were also significantly (P ≤ 0.05-0.01) improved.
  
  
  CONCLUSIONS
  The present study suggests that Plumeria alba L. has protective activity against arthritis and supports the traditional use of P. alba for rheumatism and other inflammatory diseases.
authors.affiliation: Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana 136119, India.
authors.forename: Manjusha
authors.identifier: 0000-0002-0320-9382
authors.initials: M
authors.lastname: Choudhary
authors.affiliation: Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana 136119, India ; Department of Pharmacy, School of Chemical Science and Pharmacy, Central University of Rajasthan, Ajmer 305801, India.
authors.forename: Vipin
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Dabur Research and Development Centre, Ghaziabad 201002, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Gupta
authors.affiliation: All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Surender
authors.initials: S
authors.lastname: Singh
mesh_terms: D000818:Animals; D029062:Apocynaceae / Q000737:chemistry; D001168:Arthritis / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats
chemical_list: D010936:Plant Extracts