title: Forced expression of FLO11 confers pellicle-forming ability and furfural tolerance on Saccharomyces cerevisiae in ethanol production.
abstract: We constructed a plasmid that expresses FLO11 encoding a cell surface glycoprotein of Saccharomyces cerevisiae under the control of a constitutive promoter. This plasmid conferred pellicle-forming ability on the non-pellicle-forming industrial strain of S. cerevisiae at the air-liquid interface of the glucose-containing liquid medium. The induced pellicle-forming cells exhibited tolerance to furfural, which is a key toxin in lignocellulosic hydrolysates, in ethanol production.
authors.affiliation: a Division of Biotechnology, Interdisciplinary Graduate School of Medicine and Engineering , University of Yamanashi , 4-3-11 Takeda, Kofu, Yamanashi, 400-8511 , Japan.
authors.forename: Youji
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Toru
authors.initials: T
authors.lastname: Hasebe
authors.forename: Miyuki
authors.initials: M
authors.lastname: Ishiai
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamura
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Iimura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hayakawa
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005662:Furaldehyde / Q000494:pharmacology*; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
chemical_list: C110690:FLO11 protein, S cerevisiae; D008562:Membrane Glycoproteins; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D005662:Furaldehyde; D005947:Glucose
keywords: FLO11; Saccharomyces cerevisiae; bioethanol; furfural; pellicle formation