title: DESI analysis of mammalian cell cultures--preparation and method optimisation.
abstract: Desorption electrospray ion source (DESI) is widely used as an MS imaging technique. It is a rapid and convenient method of surface analysis, but to date, there are methodological obstacles to its application to the analysis of cell culture. This study reported optimised conditions for the analysis of cell culture samples. Parameters such as the surface, medium removal and sample desiccation techniques were assessed as a function of output data quality. Supercharging agents, surfactants and optimal parameters for the DESI ion source were evaluated for use in cell culture analyses. Data indicated that plastic dishes or sodium glass coated with poly-l-lysine and washing cell cultures with 150 mM ammonium acetate followed by drying with inert gas were superior for DESI analyses. The addition of 1 μM surfactin to the DESI spray solvent significantly improved the results for negative and positive ion modes.
authors.affiliation: Department of Biochemistry and Neurobiology, Faculty of Materials Sciences and Ceramics, AGH University of Science and Technology, Mickiewicza 30 ave, 30-059, Krakow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Bodzon-Kulakowska
authors.forename: Anna
authors.initials: A
authors.lastname: Drabik
authors.forename: Marta
authors.initials: M
authors.lastname: Marszalek
authors.forename: Jolanta Helena
authors.initials: JH
authors.lastname: Kotlinska
authors.forename: Piotr
authors.initials: P
authors.lastname: Suder
mesh_terms: D000085:Acetates; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002460:Cell Line; D000432:Methanol; D051379:Mice; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013499:Surface Properties; D013501:Surface-Active Agents
chemical_list: D000085:Acetates; D013501:Surface-Active Agents; C018824:ammonium acetate; D000432:Methanol
keywords: MS imaging; cell culture; desorption electrospray; optimisation; sample preparation