title: Unsaturated long-chain fatty acids inhibit the binding of oxidized low-density lipoproteins to a model CD36.
abstract: Transmembrane protein CD36 binds multiple ligands, including oxidized low-density lipoproteins (oxLDLs) and long-chain fatty acids (LCFAs). Our aim was to determine whether LCFAs compete with oxLDLs for binding to CD36. We addressed this issue by examining the inhibitory effect of LCFAs against the binding of Alexa-fluor-labeled oxLDLs (AFL-oxLDL) to a synthetic peptide representing the oxLDL-binding site on CD36 (3S-CD36₁₅₀₋₁₆₈). All of the unsaturated LCFAs tested, inhibited the binding of AFL-oxLDL to 3S-CD36₁₅₀₋₁₆₈, albeit to varying degrees. For instance, the concentrations required for 50% inhibition of binding for oleic, linoleic, and α-linolenic acids were 0.25, 0.97, and 1.2 mM, respectively. None of the saturated LCFAs tested (e.g. stearic acid) exhibited inhibitory effects. These results suggest that at least unsaturated LCFAs can compete with oxLDLs for binding to CD36. The study also provides information on the structural requirements of LCFAs for inhibition of oxLDLs-CD36 binding.
authors.affiliation: a Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Marie
authors.initials: M
authors.lastname: Takai
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kozai
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsuzuki
authors.forename: Yukari
authors.initials: Y
authors.lastname: Matsuno
authors.forename: Maiko
authors.initials: M
authors.lastname: Fujioka
authors.forename: Kozue
authors.initials: K
authors.lastname: Kamei
authors.forename: Hitomi
authors.initials: H
authors.lastname: Inagaki
authors.forename: Ai
authors.initials: A
authors.lastname: Eguchi
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Matsumura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Inoue
authors.forename: Tohru
authors.initials: T
authors.lastname: Fushiki
mesh_terms: D000595:Amino Acid Sequence; D018955:CD36 Antigens / Q000737:chemistry / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry* / Q000494:pharmacology*; D020404:Glycerophospholipids / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding
chemical_list: D018955:CD36 Antigens; D005231:Fatty Acids, Unsaturated; D020404:Glycerophospholipids; D008077:Lipoproteins, LDL; D010446:Peptide Fragments; C099252:oxidized low density lipoprotein
keywords: CD36; binding inhibition; long-chain fatty acids; oxidized low-density lipoproteins; synthetic peptide