title: Voltage-gated potassium+ channel expression in coronary artery smooth muscle cells of SHR and WKY.
abstract: This study aims to compare the expression of genes and the molecular characteristic of voltage-gated K(+) channels, which make great effort in maintaining and controlling smooth muscle contraction, cellular membrane potential, and intracellular calcium ion currents in artery smooth muscle cells of SHR and WKY. Expression of potassium ions family in coronary artery was detected through reverse transcription polymerase chain reaction quantitatively. Significant levels of voltage-gated K(+) channels α1.2, α1.5, and β1.1 expression were all proved to be significantly higher in smooth muscles of SHR than WKY. Whole-cell voltage-gated K(+) channel currents were larger in SHR artery smooth muscles than the ones of WKY. Moreover, the voltage dependence of voltage-gated potassium channel activation was more negative in artery smooth muscle of SHR than that of WKY, while voltage dependence of availability was not different. The above diversity of voltage-gated potassium channel detected in gene expression and electrical character in coronary artery smooth muscle of SHR than that of WKY might be an underling mechanism associated with the membrane potential depolarization in artery smooth muscle of SHR.
authors.affiliation: Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, 710061, Shaanxi, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Hu
authors.forename: Aiqun
authors.initials: A
authors.lastname: Ma
authors.forename: Yushun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yutao
authors.initials: Y
authors.lastname: Xi
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
authors.forename: Tingzhong
authors.initials: T
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003331:Coronary Vessels / Q000166:cytology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D018408:Patch-Clamp Techniques; D026902:Potassium Channel Blockers / Q000494:pharmacology; D024642:Potassium Channels, Voltage-Gated / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D026902:Potassium Channel Blockers; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger