title: KIF20A regulates porcine oocyte maturation and early embryo development.
abstract: KIF20A (Kinesin-like family member 20A), also called mitotic kinesin-like proteins 2 (MKLP2), is a mammalian mitotic kinesin-like motor protein of the Kinesin superfamily proteins (KIFs), which was originally involved in Golgi apparatus dynamics and thought to essential for cell cycle regulation during successful cytokinesis. In the present study, we investigated whether KIF20A has roles on porcine oocyte meiotic maturation and subsequent early embryo development. By immunofluorescence staining, KIF20A was found to exhibit a dynamic localization pattern during meiosis. KIF20A was restricted to centromeres after germinal vesicle breakdown (GVBD), transferred to the midbody at telophase I (TI), and again associated with centromeres at metaphase II (MII). Inhibition of endogenous KIF20A via a specific inhibitor, Paprotrain, resulted in failure of polar body extrusion. Further cell cycle analysis showed that the percentage of oocytes that arrested at early metaphase I (MI) stage increased after KIF20A activity inhibition; however, the proportion of oocytes at anaphase/telophase I (ATI) and MII stages decreased significantly. Our results also showed that KIF20A inhibition did not affect spindle morphology. In addition, KIF20A was localized at the nucleus of early embryos, and KIF20A inhibition resulted in failure of early parthenogenetic embryo development. These results demonstrated that KIF20A is critical for porcine oocyte meiotic maturation and subsequent early embryo development.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, China; College of Animal Sciences and Technology, Nanjing Agricultural University, Jiangsu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Sciences and Technology, Nanjing Agricultural University, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Peng
authors.affiliation: College of Animal Sciences and Technology, Nanjing Agricultural University, Jiangsu, China.
authors.forename: Cheng-Cheng
authors.initials: CC
authors.lastname: Zhu
authors.affiliation: College of Animal Sciences and Technology, Nanjing Agricultural University, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.affiliation: Technology Centre of Guangxi Entry-Exit Inspection and Quarantine Bureau, Nanning, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Rui
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology*; D016547:Kinesins / Q000378:metabolism*; D008540:Meiosis / Q000502:physiology; D008677:Metaphase / Q000502:physiology; D059705:Polar Bodies / Q000378:metabolism* / Q000502:physiology*; D013552:Swine; D013692:Telophase / Q000502:physiology
chemical_list: D018797:Cell Cycle Proteins; D016547:Kinesins