title: Rapid, sensitive and specific detection of OXA-48-like-producing Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: The emergence of Enterobacteriaceae producing the class D carbapenemase OXA-48 has become a public health concern, particularly in southern Europe and in the Mediterranean area. The existing MALDI-TOF MS assays that detect carbapenemase activity have disregarded the Enterobacteriaceae producing OXA-48-like enzymes. Here, we assessed the ability of an optimized MALDI-TOF MS imipenem hydrolysis assay to detect OXA-48-like-producing Enterobacteriaceae. We tested 372 non duplicate isolates of Enterobacteriaceae from 9 species, among which 92 harbored a blaOXA-48-like gene and 180 were non-susceptible to ertapenem. The vast majority (98.9%) of the OXA-48-like producers were detected and 97.8% of the ertapenem non-susceptible isolates were correctly classified. From the agar plate to the final result, the turnaround time of the test was 90min. This quick, easy and accurate MALDI-TOF MS test can expedite the implementation of hygiene measures to prevent dissemination of OXA-48-like-producing Enterobacteriaceae and limit outbreaks.
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France; Centre de Ressources Biologiques - Ferdinand Cabanne, Centre Hospitalier Régional Universitaire, Besançon, France.
authors.forename: Marlène
authors.initials: M
authors.lastname: Sauget
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France.
authors.forename: Nadège
authors.initials: N
authors.lastname: Cabrolier
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Manzoni
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France; Centre de Ressources Biologiques - Ferdinand Cabanne, Centre Hospitalier Régional Universitaire, Besançon, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Bertrand
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France; Centre de Ressources Biologiques - Ferdinand Cabanne, Centre Hospitalier Régional Universitaire, Besançon, France. Electronic address: dhocquet@chu-besancon.fr.
authors.forename: Didier
authors.initials: D
authors.lastname: Hocquet
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism*; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D015378:Imipenem / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; D001618:beta-Lactamases; C108449:oxacillinase
keywords: Carbapenemase; Infection control; Klebsiella pneumoniae; MALDI-TOF MS