title: Evaluation of fractional photothermolysis effect in a mouse model using nonlinear optical microscopy.
abstract: Fractional photothermolysis (FP) induces discrete columns of photothermal damage in skin dermis, thereby promoting collagen regeneration. This technique has been widely used for treating wrinkles, sun damage, and scar. In this study, we evaluate the potential of multiphoton microscopy as a noninvasive imaging modality for the monitoring of skin rejuvenation following FP treatment. The dorsal skin of a nude mouse underwent FP treatment in order to induce microthermal zones (MTZs). We evaluated the effect of FP on skin remodeling at 7 and 14 days after treatment. Corresponding histology was performed for comparison. After 14 days of FP treatment at 10 mJ, the second harmonic generation signal recovered faster than the skin treated with 30 mJ, indicating a more rapid regeneration of dermal collagen at 10 mJ. Our results indicate that nonlinear optical microscopy is effective in detecting the damaged areas of MTZ and monitoring collagen regeneration following FP treatment.
authors.affiliation: National Taiwan University, Department of Physics, 1 Roosevelt Road, Section 4, Taipei 106, Taiwan.
authors.forename: Han Wen
authors.initials: HW
authors.lastname: Guo
authors.affiliation: National Taiwan University, Department of Physics, 1 Roosevelt Road, Section 4, Taipei 106, Taiwan.
authors.forename: Te-Yu
authors.initials: TY
authors.lastname: Tseng
authors.affiliation: National Taiwan University, Department of Physics, 1 Roosevelt Road, Section 4, Taipei 106, TaiwanbNational Taiwan University, Center for Quantum Science and Engineering, 1 Roosevelt Road, Section 4, Taipei 106, TaiwancNational Taiwan University, Center f.
authors.forename: Chen-Yuan
authors.initials: CY
authors.lastname: Dong
authors.affiliation: Cathay General Hospital, Department of Dermatology, 280 Renai Road, Section 4, Taipei 106, Taiwan.
authors.forename: Tsung-Hua
authors.initials: TH
authors.lastname: Tsai
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D003357:Cosmetic Techniques; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods*; D010789:Phototherapy / Q000379:methods*; D012867:Skin / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects
chemical_list: D003094:Collagen