title: Equine herpesvirus type 1 pUL56 modulates innate responses of airway epithelial cells.
abstract: Recently, the product of equine herpesvirus type 1 (EHV-1) ORF1, a homolog to HSV-1 pUL56, was shown to modulate MHC-I expression and innate immunity. Here, we investigated modulation of respiratory epithelial immunity by EHV-1 pUL56 and compared responses to those of PBMCs, which are important target cells that allow cell-associated EHV-1 viremia. The salient observations are as follows: (i) EHV-1 significantly down-modulated MHC-I and MHC-II expression in equine respiratory epithelial cells (ERECs). MHC-I expression remained unaffected in PBMCs and MHC-II expression was increased. (ii) Infection with an EHV-1 ORF1 deletion mutant partially restored MHC-I and MHC-II expression and altered IFN-alpha and IL-10 mRNA expression. (iii) Deletion of EHV-1 ORF1 also significantly increased chemokine expression and chemotaxis of monocytes and neutrophils in ERECs. Collectively, these results suggest a role for EHV-1 pUL56 in modulation of antigen presentation, cytokine expression and chemotaxis at the respiratory epithelium, but not in PBMC.
authors.affiliation: Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, East Lansing, MI, USA. Electronic address: husseygi@msu.edu.
authors.forename: Gisela
authors.initials: G
authors.lastname: Soboll Hussey
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
authors.forename: Laura V
authors.initials: LV
authors.lastname: Ashton
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
authors.forename: Ayshea M
authors.initials: AM
authors.lastname: Quintana
authors.affiliation: Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
authors.forename: Gerlinde R
authors.initials: GR
authors.lastname: Van de Walle
authors.affiliation: Institut für Virologie, Freie Universität Berlin, Germany.
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Osterrieder
authors.affiliation: North Carolina State University, Raleigh, NC, USA.
authors.forename: David P
authors.initials: DP
authors.lastname: Lunn
mesh_terms: D000818:Animals; D018925:Chemokines / Q000235:genetics / Q000276:immunology; D004847:Epithelial Cells / Q000276:immunology* / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000235:genetics / Q000276:immunology*; D006734:Horse Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology; D006736:Horses; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology; D020545:Respiratory Mucosa / Q000166:cytology / Q000276:immunology* / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D018925:Chemokines; D017361:Viral Nonstructural Proteins
keywords: Equine herpesvirus-1; Innate immunity; Respiratory epithelium; pUL56