title: Cytotoxic effects of the compound cis-tetraammine(oxalato)ruthenium(III) dithionate on K-562 human chronic myelogenous leukemia cells.
abstract: Chemotherapy is a common treatment for leukemia. Ruthenium complexes have shown potential utility in chemotherapy and photodynamic therapy. The identification of new chemotherapeutics agents is critical for further progress in the treatment of leukemia. Ruthenium complexes generally have lower toxicities compared to cisplatin attributed to their specific accumulation in cancer tissues. Based on these evidences, in the present work we studied the cytotoxic activity of the ruthenium(III) compound cis-tetraammine(oxalato)ruthenium(III) dithionate - {cis-[Ru(C2O4)(NH3)4]2(S2O6)} against human chronic myelogenous leukemia cells (K-562) tumor cell line. The tested compound induces cell death in a dose and time dependent manner on K-562 cells. It is found that the effect was improved linearly while prolonging the incubation time. Compared to the cell cycle profiles of untreated cells, flow cytometric analysis indicated the sub-G1 arresting effect of ruthenium compound on K-562 cells. In our study, {cis-[Ru(C2O4)(NH3)4]2(S2O6)} shows a significant increase in tailed cells in any of the concentrations tested compared with negative control. Consequently, the concentration of {cis-[Ru(C2O4)(NH3)4]2(S2O6)} might be associated cytotoxicity with direct effect on K-562 cells DNA. Thus, it can be deducted that ruthenium-based compounds present selectivity to enter both tumor and normal cells. Additional studies are needed to determine the molecular mechanisms of the active components and to evaluate the potential in vivo anticancer activity of the cis-tetraammine(oxalato)ruthenium(III) dithionate.
authors.affiliation: Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas, Universidade Federal de Goiás - UFG, Campus Samambaia (Campus II), Cx. Postal: 131, Goiânia, GO 74001-970 Brazil.
authors.forename: Flávia de Castro
authors.initials: Fde C
authors.lastname: Pereira
authors.affiliation: Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas, Universidade Federal de Goiás - UFG, Campus Samambaia (Campus II), Cx. Postal: 131, Goiânia, GO 74001-970 Brazil.
authors.forename: Aliny Pereira
authors.initials: AP
authors.lastname: de Lima
authors.affiliation: Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas, Universidade Federal de Goiás - UFG, Campus Samambaia (Campus II), Cx. Postal: 131, Goiânia, GO 74001-970 Brazil.
authors.forename: Cesar Augusto Sam Tiago
authors.initials: CA
authors.lastname: Vilanova-Costa
authors.affiliation: Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas, Universidade Federal de Goiás - UFG, Campus Samambaia (Campus II), Cx. Postal: 131, Goiânia, GO 74001-970 Brazil.
authors.forename: Wanessa Carvalho
authors.initials: WC
authors.lastname: Pires
authors.affiliation: Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas, Universidade Federal de Goiás - UFG, Campus Samambaia (Campus II), Cx. Postal: 131, Goiânia, GO 74001-970 Brazil.
authors.forename: Alessandra de Santana Braga Barbosa
authors.initials: Ade S
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas, Universidade Federal de Goiás - UFG, Campus Samambaia (Campus II), Cx. Postal: 131, Goiânia, GO 74001-970 Brazil.
authors.forename: Lucas Carlos Gomes
authors.initials: LC
authors.lastname: Pereira
authors.affiliation: Instituto de Química, Universidade Federal de Uberlândia - UFU, Uberlândia, MG 38400-902 Brazil.
authors.forename: Luiz Alfredo
authors.initials: LA
authors.lastname: Pavanin
authors.affiliation: Instituto de Ciências Exatas e da Terra, Universidade Federal de Mato Grosso - UFMT, Barra do Garças, MT 78698-000 Brazil.
authors.forename: Wagner Batista
authors.initials: WB
authors.lastname: Dos Santos
authors.affiliation: Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas, Universidade Federal de Goiás - UFG, Campus Samambaia (Campus II), Cx. Postal: 131, Goiânia, GO 74001-970 Brazil.
authors.forename: Elisângela de Paula
authors.initials: Ede P
authors.lastname: Silveira-Lacerda
keywords: Apoptosis; Cytotoxic activity; Immunomodulatory activity; K-562; Ruthenium(III) compounds; cis-tetraammine(oxalato)ruthenium(III) dithionate