title: A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry.
abstract: A label-free double amplification system has been developed by using a ternary DNA probe containing the poly(adenine-thymine) sequence assisted by exonuclease III degradation. The method achieved more than 600-fold signal amplification and allowed sensitive detection of single-stranded DNA and thrombin at the pM level by using liquid chromatography/mass spectrometry.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn hluo@pku.edu.cn.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qin
authors.forename: Chengsen
authors.initials: C
authors.lastname: Zhang
authors.forename: Meiping
authors.initials: M
authors.lastname: Zhao
authors.forename: Hai
authors.initials: H
authors.lastname: Luo
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001483:Base Sequence; D002853:Chromatography, Liquid / Q000379:methods; D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D013917:Thrombin / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D015342:DNA Probes; D004277:DNA, Single-Stranded; C081676:thrombin aptamer; D013917:Thrombin