title: Identification and characterization of a novel phage display-derived peptide with affinity for human brain metastatic breast cancer.
abstract: A novel peptide, BRBP1 (MYPWTEPSYLSN), was identified using an in vitro phage biopanning strategy against human brain-seeking breast carcinoma cells (231-BR cells).The peptide-phage clone, BRBP1-M13 displaying BRBP1 sequence, specifically bound to 231-BR cells and the binding could be competitively abolished by BRBP1. In vivo distribution studies showed that BRBP1-M13 preferentially homed to the 231-BR tumors. Fluorescently-labeled BRBP1, BRBP1-K(5-TAMRA), preferentially bound to 231-BR cells in a dose-dependent and energy-dependent manner and it was efficiently internalized into the cells after 2 h incubation. Near-infrared fluorophores imaging demonstrated the accumulation of Cy5.5-conjugated BRBP1 peptide in the tumors in vivo. Thus, BRBP1 is a promising peptide binding to human brain metastatic breast cancer and it may be applied to targeted delivery of cytotoxic agents to the intended tumor.
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease, Ministry of Education; Department of Microbiology and Immunology, Medical School Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, Jiangsu, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Long
authors.forename: Aifeng
authors.initials: A
authors.lastname: Zhang
authors.forename: Yafen
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanli
authors.initials: Y
authors.lastname: An
authors.forename: Fengqin
authors.initials: F
authors.lastname: Miao
authors.forename: Fang
authors.initials: F
authors.lastname: Nie
authors.forename: Mingli
authors.initials: M
authors.lastname: Li
authors.forename: Youji
authors.initials: Y
authors.lastname: He
authors.forename: Jianqiong
authors.initials: J
authors.lastname: Zhang
authors.forename: Gaojun
authors.initials: G
authors.lastname: Teng
mesh_terms: D000595:Amino Acid Sequence; D001932:Brain Neoplasms / Q000378:metabolism* / Q000556:secondary; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D019151:Peptide Library; D010455:Peptides