title: A virulent bioluminescent and fluorescent dual-reporter Marek's disease virus unveils an alternative spreading pathway in addition to cell-to-cell contact.
abstract: Marek's disease virus (MDV) is a growing threat for the poultry industry. Unfortunately, despite successful vaccination against the disease, MDV remains in circulation within vaccinated flocks, leading to the selection of increasingly virulent pathotypes. Detailed knowledge of the virus biology and the host-virus interaction is required to improve the vaccine efficiency. In the present study, I engineered an original, dual-reporter MDV to track and quantify virus replication in vitro and in vivo.
authors.affiliation: Institut National de la Recherche Agronomique (INRA), Centre Val de Loire, Nouzilly, France Université François Rabelais, UMR1282 Infectiologie et Santé Publique, Tours, France abdallah.harmache@tours.inra.fr.
authors.forename: Abdallah
authors.initials: A
authors.lastname: Harmache
mesh_terms: D000818:Animals; D002450:Cell Communication; D045744:Cell Line, Tumor; D002645:Chickens; D017930:Genes, Reporter; D016679:Genome, Viral; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements; D008380:Marek Disease / Q000401:mortality / Q000473:pathology / Q000821:virology*; D008856:Microscopy, Fluorescence; D011401:Promoter Regions, Genetic; D016865:Reassortant Viruses / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D016019:Survival Analysis; D013552:Swine; D052700:Teschovirus / Q000235:genetics; D014774:Virulence; D014779:Virus Replication
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D008156:Luciferases