title: Disseminated Kaposi's sarcoma in patients with HIV infection correlates to high serum levels of IL-10.
abstract: Human herpesvirus 8 (HHV-8) is the etiologic agent of all Kaposi's sarcoma (KS), the outcome of which is associated with immuno-dysregulation, resulting in the abnormal production of inflammatory cytokines and chemokines. We quantified by enzyme-linked immunosorbent assay serum levels of interleukin (IL)-10, IL-17, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α from patients with KS-AIDS, classic KS, and human immunodeficiency virus (HIV) without KS. A correlation between HHV-8 molecular detection and cytokine production was also performed. We observed that IL-10 production was higher in patients with KS-AIDS when compared to those with classic KS or HIV. However, no significant differences were seen for IFN-γ, TNF-α, or IL-17 production between studied groups. When patients with KS-AIDS were analyzed according to lesion topography, IL-10 levels were higher in patients with disseminated disease than those observed in patients with only cutaneous lesions or cutaneous and digestive and/or respiratory tract lesions. Finally, patients with KS-AIDS that presented viral DNA for HHV-8 in serum showed a higher production of IL-10 when compared with those patients with a negative result for nested polymerase chain reaction for the virus. The results presented here are the first to demonstrate that there exists a stratification of patients with KS-AIDS according to lesion topography where IL-10 levels are higher in those individuals with disseminated disease than those with only localized lesions.
authors.affiliation: 1 Department of Internal Medicine, Virology Research Center, School of Medicine of Ribeirão Preto, University of São Paulo , Ribeirão Preto, São Paulo, Brazil .
authors.forename: Paula Renata Lima
authors.initials: PR
authors.lastname: Machado
authors.forename: Kleber Juvenal Silva
authors.initials: KJ
authors.lastname: Farias
authors.forename: Julieta
authors.initials: J
authors.lastname: Genre
authors.forename: Carlo José Freire
authors.initials: CJ
authors.lastname: Oliveira
authors.forename: Paulo Marcos Matta
authors.initials: PM
authors.lastname: Guedes
authors.forename: Benedito Antônio Lopes
authors.initials: BA
authors.lastname: da Fonseca
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D005786:Gene Expression Regulation / Q000276:immunology; D015658:HIV Infections / Q000097:blood* / Q000150:complications* / Q000276:immunology; D019288:Herpesvirus 8, Human / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D012307:Risk Factors; D012514:Sarcoma, Kaposi / Q000097:blood* / Q000150:complications* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D016753:Interleukin-10