title: DETECTION OF LACTOBACILLI IN MONTHLY MAIL-IN STOOL SAMPLES FROM 3-18 MONTHS OLD INFANTS AT GENETIC RISK FOR TYPE 1 DIABETES.
abstract: The feasibility to detect lactobacilli in mail-in infant stools collected monthly from 3-18 months old children was investigated. The aim was to determine total lactobacilli and Lactobacillus plantarum (L. plantarum) content (ng/g feces) in 50 infants each from Colorado (648 samples), Finland (624 samples) and Sweden (685 samples) who participated in the TEDDY (The Environmental Determinants of Diabetes in the Young) study. Total lactobacilli content varied markedly between 5 and 16,800 ng/g feces in the three clinical sites within and between individuals especially in infants. L.plantarum also varied markedly intra- and inter-individually from <0.5 - 736 ng/g feces. A higher variability of total lactobacilli was found before 10 months of age than after in the three different clinical sites. Sweden had the lowest total lactobacilli content compared to Colorado and Finland while the L.plantarum content was higher in Sweden. Mail-in stool samples from infants should prove useful in analyzing probiotics in childhood.
authors.affiliation: Department of Clinical Sciences, Lund University/CRC, Skåne University Hospital SUS, Malmö, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Salami
authors.affiliation: Department of Clinical Sciences, Lund University/CRC, Skåne University Hospital SUS, Malmö, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Abels
authors.affiliation: Department of Virology, Tampere University Hospital, Tampere, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Hyöty
authors.affiliation: Department of Clinical Sciences, Lund University/CRC, Skåne University Hospital SUS, Malmö, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Vaziri-Sani
authors.affiliation: Department of Clinical Sciences, Lund University/CRC, Skåne University Hospital SUS, Malmö, Sweden.
authors.forename: Ca
authors.initials: C
authors.lastname: Aronsson
authors.affiliation: Morsani College of Medicine, Pediatrics Epidemiology Center, University of South Florida, Tampa, FL, USA.
authors.forename: K
authors.initials: K
authors.lastname: Vehik
authors.affiliation: Department of Clinical Sciences, Lund University/CRC, Skåne University Hospital SUS, Malmö, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Delli
authors.affiliation: Pacific Northwest Diabetes Research Institute, Seattle, WA, USA.
authors.forename: Wa
authors.initials: W
authors.lastname: Hagopian
authors.affiliation: Barbara Davis Center for Childhood Diabetes, University of Colorado, Aurora, CO, USA.
authors.forename: M
authors.initials: M
authors.lastname: Rewers
authors.affiliation: Diabetes Research Institute, Technische Universität München, Munich, Germany.
authors.forename: Ag
authors.initials: A
authors.lastname: Ziegler
authors.affiliation: Department of Pediatrics, University of Turku, Turku, Finland.
authors.forename: O
authors.initials: O
authors.lastname: Simell
authors.affiliation: DDEMD, NIDDK, Bethesda, MD, USA.
authors.forename: B
authors.initials: B
authors.lastname: Akolkar
authors.affiliation: Morsani College of Medicine, Pediatrics Epidemiology Center, University of South Florida, Tampa, FL, USA.
authors.forename: J
authors.initials: J
authors.lastname: Krischer
authors.affiliation: Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta, GA, USA.
authors.forename: J
authors.initials: J
authors.lastname: She
authors.affiliation: Department of Clinical Sciences, Lund University/CRC, Skåne University Hospital SUS, Malmö, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Lernmark
keywords: Autoimmune Diabetes; Celiac Disease; Infants; Microflora; Probiotics