title: Identification and characterization of thermotolerant acetic acid bacteria strains isolated from coconut water vinegar in Sri Lanka.
abstract: From the pellicle formed on top of brewing coconut water vinegar in Sri Lanka, three Acetobacter strains (SL13E-2, SL13E-3, and SL13E-4) that grow at 42 °C and four Gluconobacter strains (SL13-5, SL13-6, SL13-7, and SL13-8) grow at 37 °C were identified as Acetobacter pasteurianus and Gluconobacter frateurii, respectively. Acetic acid production by the isolated Acetobacter strains was examined. All three strains gave 4% acetic acid from 6% initial ethanol at 37 °C, and 2.5% acetic acid from 4% initial ethanol at 40 °C. Compared with the two other strains, SL13E-4 showed both slower growth and slower acetic acid production. As well as the thermotolerant SKU1108 strain, the activities of the alcohol dehydrogenase and the aldehyde dehydrogenase of SL13E-2 and SL13E-4 were more stable than those of the mesophilic strain. The isolated strains were used to produce coconut water vinegar at higher temperatures than typically used for vinegar production.
authors.affiliation: a United Graduate School of Agricultural Science, Kagoshima University , Kagoshima , Japan.
authors.forename: P A B N
authors.initials: PA
authors.lastname: Perumpuli
authors.forename: Taisuke
authors.initials: T
authors.lastname: Watanabe
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
mesh_terms: D019342:Acetic Acid / Q000737:chemistry / Q000378:metabolism*; D000426:Alcohol Dehydrogenase / Q000737:chemistry; D000444:Aldehyde Dehydrogenase / Q000737:chemistry; D003059:Cocos / Q000382:microbiology*; D004795:Enzyme Stability; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D020576:Gluconobacter / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D006358:Hot Temperature; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013188:Sri Lanka
chemical_list: D012336:RNA, Ribosomal, 16S; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase; D019342:Acetic Acid
keywords: Acetobacter pasteurianus; Gluconobacter frateurii; acetic acid bacteria (AAB); coconut water vinegar; thermotolerant bacteria