title: Cereal byproducts have prebiotic potential in mice fed a high-fat diet.
abstract: Barley husks, rye bran, and a fiber residue from oat milk production were processed by heat pretreatment, various separation steps, and treatment with an endoxylanase in order to improve the prebiotic potential of these cereal byproducts. Metabolic functions were intended to improve along with improved microbial activity. The products obtained were included in a high-fat mouse diet so that all diets contained 5% dietary fiber. In addition, high-fat and low-fat controls as well as partially hydrolyzed guar gum were included in the study. The soluble fiber product obtained from rye bran caused a significant increase in the bifidobacteria (log copies of 16S rRNA genes; median (25-75 percentile): 6.38 (6.04-6.66) and 7.47 (7.30-7.74), respectively; p < 0.001) in parallel with a tendency of increased production of propionic acid and indications of improved metabolic function compared with high-fat fed control mice. The oat-derived product caused an increase in the pool of cecal propionic (from 0.62 ± 0.12 to 0.94 ± 0.08) and butyric acid (from 0.38 ± 0.04 to 0.60 ± 0.04) compared with the high-fat control, and it caused a significant increase in lactobacilli (log copies of 16S rRNA genes; median (25-75 percentile): 6.83 (6.65-7.53) and 8.04 (7.86-8.33), respectively; p < 0.01) in the cecal mucosa. However, no changes in measured metabolic parameters were observed by either oat or barley products.
authors.affiliation: Biomedical Center, Department of Experimental Medical Science, Lund University , BMC C11, Lund SE-22184, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Berger
authors.forename: Peter
authors.initials: P
authors.lastname: Falck
authors.forename: Caroline
authors.initials: C
authors.lastname: Linninge
authors.forename: Ulf
authors.initials: U
authors.lastname: Nilsson
authors.forename: Ulrika
authors.initials: U
authors.lastname: Axling
authors.forename: Carl
authors.initials: C
authors.lastname: Grey
authors.forename: Henrik
authors.initials: H
authors.lastname: Stålbrand
authors.forename: Eva
authors.initials: E
authors.lastname: Nordberg Karlsson
authors.forename: Margareta
authors.initials: M
authors.lastname: Nyman
authors.forename: Cecilia
authors.initials: C
authors.lastname: Holm
authors.forename: Patrick
authors.initials: P
authors.lastname: Adlercreutz
mesh_terms: D000818:Animals; D018554:Avena / Q000737:chemistry*; D001644:Bifidobacterium / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D002432:Cecum / Q000378:metabolism / Q000382:microbiology; D059305:Diet, High-Fat / Q000009:adverse effects; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005525:Food-Processing Industry / Q000191:economics; D001467:Hordeum / Q000737:chemistry*; D006358:Hot Temperature; D006868:Hydrolysis; D007220:Industrial Waste / Q000032:analysis* / Q000191:economics; D007333:Insulin Resistance; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology; D007777:Lactobacillaceae / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000178:diet therapy* / Q000209:etiology / Q000378:metabolism / Q000382:microbiology; D056692:Prebiotics / Q000191:economics; D012434:Secale / Q000737:chemistry*; D013548:Sweden
chemical_list: D005232:Fatty Acids, Volatile; D007220:Industrial Waste; D056692:Prebiotics