title: Pseudomonas aeruginosa biofilm growth inhibition on medical plastic materials by immobilized esterases and acylase.
abstract: Biofilms are matrix-encapsulated cell aggregates that cause problems in technical and health-related areas; for example, 65 % of all human infections are biofilm associated. This is mainly due to their ameliorated resistance against antimicrobials and immune systems. Pseudomonas aeruginosa, a biofilm-forming organism, is commonly responsible for nosocomial infections. Biofilm development is partly mediated by signal molecules, such as acyl-homoserine lactones (AHLs) in Gram-negative bacteria. We applied horse liver esterase, porcine kidney acylase, and porcine liver esterase; these can hydrolyze AHLs, thereby inhibiting biofilm formation. As biofilm infections are often related to foreign material introduced into the human body, we immobilized the enzymes on medical plastic materials. Biofilm formation was quantified by Crystal Violet staining and confocal laser scanning microscopy, revealing up to 97 % (on silicone), 54 % (on polyvinyl chloride), and 77 % (on polyurethane) reduced biomass after 68 h growth.
authors.affiliation: Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestr. 15, 21071 Hamburg (Germany).
authors.forename: Johannes Martin
authors.initials: JM
authors.lastname: Kisch
authors.forename: Christian
authors.initials: C
authors.lastname: Utpatel
authors.forename: Lutz
authors.initials: L
authors.lastname: Hilterhaus
authors.forename: Wolfgang R
authors.initials: WR
authors.lastname: Streit
authors.forename: Andreas
authors.initials: A
authors.lastname: Liese
mesh_terms: D000581:Amidohydrolases / Q000494:pharmacology*; D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D018533:Biomass; D004800:Enzymes, Immobilized / Q000494:pharmacology*; D004950:Esterases / Q000494:pharmacology*; D006736:Horses; D008099:Liver / Q000201:enzymology; D010969:Plastics; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development*; D055672:Static Electricity; D013552:Swine
chemical_list: D004800:Enzymes, Immobilized; D010969:Plastics; D004950:Esterases; D000581:Amidohydrolases; C019665:amidase
keywords: biofilms; immobilization; lactones; polymer coating; quorum quenching