title: A quick and accurate screening method for fungal gene-deletion mutants by direct, priority-based, and inverse PCRs.
abstract: Using two-step-PCR screening which consists of direct and priority-based PCR and inverse PCR, fungal gene-deletion mutants were selected quickly and accurately. It omits genomic DNA extraction and Southern blotting steps and prevents misinterpretations caused by PCR failure. It is anticipated to facilitate large-scale reverse genetic studies in fungi.
authors.affiliation: Department of Agricultural Biotechnology, Center for Fungal Genetic Resources, Center for Fungal Pathogenesis, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: asdfppp2@gmail.com.
authors.forename: Jaejin
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Agricultural Biotechnology, Center for Fungal Genetic Resources, Center for Fungal Pathogenesis, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: ssungbum2@gmail.com.
authors.forename: Seongbeom
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Center for Fungal Genetic Resources, Center for Fungal Pathogenesis, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: kwon.seomun@gmail.com.
authors.forename: Seomun
authors.initials: S
authors.lastname: Kwon
authors.affiliation: Department of Agricultural Biotechnology, Center for Fungal Genetic Resources, Center for Fungal Pathogenesis, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: yonglee@snu.ac.kr.
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Lee
mesh_terms: D005658:Fungi / Q000235:genetics*; D005800:Genes, Fungal; D005820:Genetic Testing / Q000379:methods*; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D059386:Reverse Genetics / Q000379:methods; D017384:Sequence Deletion
keywords: Direct PCR; Fungal mutant screening; Inverse PCR; Priority-based PCR