title: ZNF217 is associated with poor prognosis and enhances proliferation and metastasis in ovarian cancer.
abstract: ZNF217 is an alternatively spliced Kruppel-like transcription factor that has recently been implicated to play a role in human carcinogenesis. Here, we used immunohistochemistry (IHC) to show that ZNF217 protein is overexpressed in nearly 60% of ovarian tumor samples. The disease-free survival time was shorter in patients with positive ZNF217 expression than in ZNF217-negative patients (P=0.042). Fluorescence in situ hybridization (FISH) analysis showed ZNF217 genomic amplification in the poorly differentiated tumors, suggesting that ZNF217 is associated with the progression of ovarian cancer. Invasion was enhanced in HO-8910 cells stably transfected with constructs carrying full-length ZNF217 relative to cells transfected with the empty vector. To confirm our findings in vivo, we performed a tumorigenicity assay in nude mice inoculated with the HO-8910 overexpressing ZNF217 cells. As expected, tumors grown in the ZNF217 group were more invasive and prone to metastasis than those formed control groups. Based on these clinical and laboratory observations, we conclude that ZNF217 may contribute to ovarian cancer invasion and metastasis, and associated with worse clinical outcomes.
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University Guangzhou, Guangdong Province 510515, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University Guangzhou, Guangdong Province 510515, China.
authors.forename: Lanlin
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University Guangzhou, Guangdong Province 510515, China.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University Guangzhou, Guangdong Province 510515, China.
authors.forename: Ailan
authors.initials: A
authors.lastname: Yin
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University Guangzhou, Guangdong Province 510515, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D049109:Cell Proliferation; D018284:Cystadenocarcinoma, Serous / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D018572:Disease-Free Survival; D005260:Female; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D053208:Kaplan-Meier Estimate; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D015534:Trans-Activators; C113698:ZNF217 protein, human
keywords: Ovarian cancer; ZNF217 gene; gene expression; proliferation and invasion; tumor metastasis