title: Decreased expression of a novel lncRNA CADM1-AS1 is associated with poor prognosis in patients with clear cell renal cell carcinomas.
abstract: The clear cell renal cell carcinoma (ccRCC) is the most common subtype in renal cell carcinomas. Rapidly accumulating studies show that the long non-coding RNAs (lncRNAs) may play essential roles in cancers. In this study, we investigated the expression pattern of a novel lncRNA CADM1-AS1 in ccRCC by quantitative real time PCR. The results showed that CADM1-AS1 expression was down-regulated in tumor tissues in 64 patients with ccRCC compared with adjacent non-tumor tissues. Furthermore, the expression of CADM1-AS1 was positively correlated with the expression of mRNA CADM1 in ccRCC specimens (R = 0.611, P <0.0001). Decreased CADM1-AS1 expression was correlated with the progression of AJCC stage (P = 0.039) and worse survival of ccRCC patients (P <0.05). Also, multivariate analysis identified low CADM1-AS1 expression as an independent prognostic factor for ccRCC (P <0.001, HR = 0.211, 95% CI = 0.088-0.504). In addition, we used small interfering RNA (siRNA) to evaluate the biological function of CADM1-AS1 in vitro. The results showed that CADM1-AS1 expression was positively associated with CADM1 mRNA expression in 786-O cells and ACHN cells. Functional experiments demonstrated markedly enhanced ability of growth and migration, and reduced apoptotic rate in CADM1-AS1 knocking down in 786-O cells. Conversely, overexpression of CADM1-AS1 showed a significant decrease in growth and migration, along with an increase in apoptotic rate in ACHN cells. In conclusion, our data demonstrated CADM1-AS1 is a new tumor suppressor in ccRCC which regulates cell proliferation, apoptosis and migration via the expression pattern of "CADM1-AS1/CADM1 mRNA gene pairs". CADM1-AS1 may be a potential biomarker and therapeutic target in patients with ccRCC.
authors.affiliation: Department of Oncology, 161 Hospital of PLA Wuhan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yao
authors.affiliation: Division of Internal Medicine, Chinese PLA General Hospital Medical School Beijing 100853, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital Medical School Beijing 100853, China.
authors.forename: Yongcheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, 253 Hospital of PLA Huhhot 010050, China.
authors.forename: Shouying
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital Medical School Beijing 100853, China.
authors.forename: Xueling
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Division of Internal Medicine, Chinese PLA General Hospital Medical School Beijing 100853, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Pan
authors.affiliation: Division of Internal Medicine, Chinese PLA General Hospital Medical School Beijing 100853, China.
authors.forename: Peiliang
authors.initials: P
authors.lastname: Geng
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis / Q000235:genetics; D053208:Kaplan-Meier Estimate; D007680:Kidney Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; C403514:CADM1 protein, human; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D062085:RNA, Long Noncoding
keywords: CADM1-AS1; clear cell renal cell carcinoma; lnc RNAs