title: Optimization of ultrasonic-assisted extraction and in vitro antioxidant activities of polysaccharides from Trametes orientalis.
abstract: A Box-Behnken design was employed to optimize ultrasonic-assisted extraction of Trametes orientalis polysaccharides (TOP). The crude polysaccharides were purified by DEAE cellulose-52 chromatography, giving a main fraction named as PTOP. The antioxidant properties of PTOP were evaluated by different in vitro antioxidant assays, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities, reducing power, superoxide radical scavenging activities, and chelating ability of ferrous ions. The results showed that optimal extraction parameters were as follows: ratio of water to raw material 30.6 mL/g, ultrasonic power 109.8 W, extraction temperature 40.2 °C, and extraction time 42.2 min. Under these conditions, the experimental yield of polysaccharides was 7.49 ± 0.14%, which agreed closely with the predicted value (7.47%). Furthermore, PTOP exhibited antioxidant capacity in a concentration-dependent manner in all assays.
authors.affiliation: College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221000, China; Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou 221000, China. Electronic address: biozheng@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221000, China. Electronic address: lishaoyong693@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221000, China. Electronic address: wwd.123@163.com.
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D045424:Complex Mixtures / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D013010:Sonication; D013481:Superoxides / Q000737:chemistry; D013696:Temperature; D055454:Trametes / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D045424:Complex Mixtures; D016166:Free Radical Scavengers; D011134:Polysaccharides; D014867:Water; D013481:Superoxides
keywords: Antioxidant; Polysaccharides; Trametes orientalis; Ultrasonic-assisted extraction