title: Quantitative evaluation of biological reaction kinetics in confined nanospaces.
abstract: Evaluating the kinetics of biological reaction occurring in confined nanospaces is of great significance in studying the molecular biological processes in vivo. Herein, we developed a nanochannel-based electrochemical reactor and a kinetic model to investigate the immunological reaction in confined nanochannels simply by the electrochemical method. As a result, except for the reaction kinetic constant that was previously studied, more insightful kinetic information such as the moving speed of the antibody and the immunological reaction progress in nanochannels were successfully revealed in a quantitative way for the first time. This study would not only pave the investigation of molecular biological processes in confined nanospaces but also be promising to extend to other fields such as biological detection and clinical diagnosis.
authors.affiliation: Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University , Nanjing 211189, China.
authors.forename: Jiachao
authors.initials: J
authors.lastname: Yu
authors.forename: Peicheng
authors.initials: P
authors.lastname: Luo
authors.forename: Chuanxian
authors.initials: C
authors.lastname: Xin
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cao
authors.forename: Yuanjian
authors.initials: Y
authors.lastname: Zhang
authors.forename: Songqin
authors.initials: S
authors.lastname: Liu
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D004077:Digoxin / Q000737:chemistry / Q000276:immunology*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D007158:Immunologic Techniques / Q000295:instrumentation*; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation*
chemical_list: D000911:Antibodies, Monoclonal; D004077:Digoxin; D004247:DNA; D000537:Aluminum Oxide