title: Structural analysis and characterization of new small serum proteins from the serum of a venomous snake (Gloydius blomhoffii).
abstract: Some snakes have several anti-toxic proteins in their sera that neutralize their own venom. Five new small serum proteins (SSPs) were isolated from Japanese mamushi (Gloydius blomhoffii) serum by gel-filtration and RP-HPLC, and their N-Terminal sequences were determined. The amino acid sequences of the precursor proteins were deduced from the nucleotide sequences of cDNAs encoding them. Due to the sequence similarity to those of SSPs in habu snake (Protobothrops flavoviridis) serum (>75% identity), these proteins were designated mSSP-1 to mSSP-5 as the homologs of habu proteins. mSSP-1 was stable at 100 °C and in the pH range of 1-10, and inhibited the proteolytic activity of a certain snake venom metalloproteinase. The inhibitory activity was extinguished by modifying the amino groups of mSSP-1. mSSP-1 is the first prostate secretory protein of the 94 amino acid-family protein with a carbohydrate chain in the Asn37 residue.
authors.affiliation: a Faculty of Science, Department of Chemistry , Fukuoka University , Jonan-ku, Fukuoka , Japan.
authors.forename: Narumi
authors.initials: N
authors.lastname: Shioi
authors.forename: Masanobu
authors.initials: M
authors.lastname: Deshimaru
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Terada
mesh_terms: D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D018076:DNA, Complementary / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012910:Snake Venoms / Q000737:chemistry / Q000235:genetics*; D017841:Trimeresurus / Q000097:blood
chemical_list: D001798:Blood Proteins; D018076:DNA, Complementary; D012910:Snake Venoms
keywords: endogenous inhibitor; self-defense system; snake venom metalloproteinase inhibitor; venomous snake serum protein