title: Raman spectroscopy of serum: A study on 'pre' and 'post' breast adenocarcinoma resection in rat models.
abstract: Risk of recurrence is a major problem in breast cancer management. Currently available prognostic markers have several disadvantages including low sensitivity and specificity, highlighting the need for new prognostic techniques. One of the candidate techniques is serum-based Raman spectroscopy (RS). In this study, feasibility of using RS to distinguish 'pre' from 'post' breast tumor resection serum in rats was explored. Spectral analysis suggests change in proteins and amino acid profiles in 'post' compared to 'pre-surgical' group. Principal-Component-Linear-Discriminant-Analysis shows 87% and 91% classification efficiency for 'pre' and 'post-surgical' groups respectively. Thus, the study further supports efficacy of RS for theranostic applications.
authors.affiliation: Chilakapati Lab, ACTREC, Tata Memorial Centre, Navi Mumbai, India.
authors.forename: Tanmoy
authors.initials: T
authors.lastname: Bhattacharjee
authors.affiliation: Chilakapati Lab, ACTREC, Tata Memorial Centre, Navi Mumbai, India.
authors.forename: Aarif
authors.initials: A
authors.lastname: Khan
authors.affiliation: Chilakapati Lab, ACTREC, Tata Memorial Centre, Navi Mumbai, India.
authors.forename: Piyush
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Laboratory Animal Facility, ACTREC, Tata Memorial Centre, Navi Mumbai, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Ingle
authors.affiliation: Maru Lab, ACTREC, Tata Memorial Centre, Navi Mumbai, India.
authors.forename: Girish
authors.initials: G
authors.lastname: Maru
authors.affiliation: Chilakapati Lab, ACTREC, Tata Memorial Centre, Navi Mumbai, India.
authors.forename: C Murali
authors.initials: CM
authors.lastname: Krishna
mesh_terms: D000230:Adenocarcinoma / Q000097:blood* / Q000601:surgery*; D000818:Animals; D016002:Discriminant Analysis; D005240:Feasibility Studies; D008325:Mammary Neoplasms, Experimental / Q000097:blood* / Q000601:surgery*; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013059:Spectrum Analysis, Raman
keywords: PC-LDA; Raman spectroscopy; animal model; breast adenocarcinomas; pre and post surgical resection; serum; tumor resection surgery