title: Composite chitosan/silk fibroin nanofibers for modulation of osteogenic differentiation and proliferation of human mesenchymal stem cells.
abstract: Nanofibrous membrane scaffolds of chitosan (CS), silk fibroin (SF) and CS/SF blend were prepared by electrospinning and studied for growth and osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs). The morphology and physico-chemical properties of all membrane scaffolds were compared. The influence of CS and SF on cell proliferation was assessed by the MTS assay, whereas osteogenic differentiation was determined from the Alizarin Red staining, alkaline phosphatase activity and expression of osteogenic marker genes. The osteogenic differentiation and proliferation of hMSCs were enhanced by CS and SF nanofibers, respectively. Blending CS with SF retained the osteogenesis nature of CS without negatively influencing the cell proliferative effect of SF. By taking advantage of the differentiation/proliferation cues from individual components, the electrospun CS/SF composite nanofibrous membrane scaffold is suitable for bone tissue engineering.
authors.affiliation: Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan, ROC.
authors.forename: Guo-Jyun
authors.initials: GJ
authors.lastname: Lai
authors.affiliation: Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan, ROC.
authors.forename: K T
authors.initials: KT
authors.lastname: Shalumon
authors.affiliation: Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan, ROC.
authors.forename: Shih-Hsien
authors.initials: SH
authors.lastname: Chen
authors.affiliation: Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan 333, Taiwan, ROC; Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Kweishan, Taoyuan 333, Taiwan, ROC. Electronic address: jpchen@mail.cgu.edu.tw.
authors.forename: Jyh-Ping
authors.initials: JP
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000201:enzymology; D002113:Calcification, Physiologic; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology; D057139:Nanofibers / Q000737:chemistry; D010012:Osteogenesis / Q000187:drug effects*; D047011:Silk / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D047011:Silk; D048271:Chitosan; D000469:Alkaline Phosphatase
keywords: Bone tissue engineering; Chitosan; Composite nanofiber; Electrospinning; Human mesenchymal stem cells; Silk fibroin