title: Lack of effect of a P2Y6 receptor antagonist on neuropathic pain behavior in mice.
abstract: Accumulating evidence indicates that various subtypes of purinergic receptors (P2X and P2Y receptor families) play an essential role in the development and the maintenance of neuropathic pain. However, there is only limited data available about the role of P2Y6 receptors in pain processing. Here we detected P2Y6 receptor immunoreactivity in primary afferent neurons of mice and observed an upregulation in response to peripheral nerve injury. However, systemic and intrathecal administration of the P2Y6 receptor antagonist MRS2578 failed to affect the injury-induced neuropathic pain behavior. Our results suggest that P2Y6 receptors, in contrast to other purinergic receptor subtypes, are not critically involved in nerve injury-induced neuropathic pain processing in mice.
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Universitätsklinikum Frankfurt, 60590 Frankfurt am Main, Germany.
authors.forename: Katharina Martina Janice
authors.initials: KM
authors.lastname: Syhr
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Universitätsklinikum Frankfurt, 60590 Frankfurt am Main, Germany.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Kallenborn-Gerhardt
authors.affiliation: Institut für Pharmakologie und Toxikologie, ZBAF, Universität Witten/Herdecke, 58453 Witten, Germany.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Lu
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Universitätsklinikum Frankfurt, 60590 Frankfurt am Main, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Olbrich
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Universitätsklinikum Frankfurt, 60590 Frankfurt am Main, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Schmitz
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Universitätsklinikum Frankfurt, 60590 Frankfurt am Main, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Männich
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Universitätsklinikum Frankfurt, 60590 Frankfurt am Main, Germany.
authors.forename: Nerea
authors.initials: N
authors.lastname: Ferreiros-Bouzas
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Universitätsklinikum Frankfurt, 60590 Frankfurt am Main, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology, Project Group Translational Medicine and Pharmacology (IME-TMP), 60590 Frankfurt am Main, Germany.
authors.forename: Gerd
authors.initials: G
authors.lastname: Geisslinger
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Universitätsklinikum Frankfurt, 60590 Frankfurt am Main, Germany.
authors.forename: Ellen
authors.initials: E
authors.lastname: Niederberger
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Universitätsklinikum Frankfurt, 60590 Frankfurt am Main, Germany; Institut für Pharmakologie und Toxikologie, ZBAF, Universität Witten/Herdecke, 58453 Witten, Germany. Electronic address: achim.schmidtko@uni-wh.de.
authors.forename: Achim
authors.initials: A
authors.lastname: Schmidtko
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D015153:Blotting, Western; D017879:Isothiocyanates / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009437:Neuralgia / Q000188:drug therapy*; D058914:Purinergic Antagonists / Q000627:therapeutic use*; D060888:Real-Time Polymerase Chain Reaction; D018048:Receptors, Purinergic P2 / Q000187:drug effects* / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism; D013890:Thiourea / Q000031:analogs & derivatives* / Q000627:therapeutic use
chemical_list: D017879:Isothiocyanates; C541384:N,N''-1,4-butanediylbis(N'-(3-isothiocyanatophenyl))thiourea; D058914:Purinergic Antagonists; D018048:Receptors, Purinergic P2; C099845:purinoceptor P2Y6; D013890:Thiourea
keywords: Dorsal root ganglia; Nerve injury; Neuropathic pain; Purinergic signaling; Spinal cord