title: Rural-urban focus of canine visceral leishmaniosis in the far western region of Santa Catarina State, Brazil.
abstract: The aim of this work was to investigate the occurrence of canine visceral leishmaniosis (CVL) in the far western region of Santa Catarina State, bordering Argentina and Parana State, southern Brazil, where in recent years, VL has been recorded in both dogs and humans. Clinical signs, enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescent antibody test (IFAT) and polymerase chain reaction (PCR) were used for Leishmania investigation. Among the 252 dogs surveyed, 41 were positive by ELISA assay, 43 in IFAT (titer>40), and 55 by PCR. From the 48 positive for VL by both serological and molecular methods, 19 (39.6%) presented clinical symptoms of leishmaniosis, 35 (72.9%) were from rural areas, and 13 (27.1%) were from urban areas. This pilot study confirms the occurrence of VL among dogs in the far western region of Santa Catarina, southern Brazil, with high risk of CVL outbreaks and presenting a threat to humans.
authors.affiliation: Programa de pós graduação em engenharia de bioprocessos e biotecnologia, Universidade Federal do Parana, Curitiba, PR, Brasil.
authors.forename: Nelí
authors.initials: N
authors.lastname: Maziero
authors.affiliation: Programa de pós graduação em engenharia de bioprocessos e biotecnologia, Universidade Federal do Parana, Curitiba, PR, Brasil. Electronic address: vanetesoccol@gmail.com.
authors.forename: Vanete
authors.initials: V
authors.lastname: Thomaz-Soccol
authors.affiliation: Departemanro de microbiologia, imunologia e parasitologia, Universidade Federal de Santa Catarina, Florianopolis, SC, Brasil.
authors.forename: Mário
authors.initials: M
authors.lastname: Steindel
authors.affiliation: Programa de pós graduação em microbiologia, parasitologia e patologia, Universidade Federal do Parana, Curitiba, PR, Brasil.
authors.forename: Juliana Seger
authors.initials: JS
authors.lastname: Link
authors.affiliation: Clínica Veterinária e Pet Shop Canis e Felis, São Miguel do Oeste, SC, Brasil.
authors.forename: Diego
authors.initials: D
authors.lastname: Rossini
authors.affiliation: Programa de pós graduação em engenharia de bioprocessos e biotecnologia, Universidade Federal do Parana, Curitiba, PR, Brasil.
authors.forename: Silvana M
authors.initials: SM
authors.lastname: Alban
authors.affiliation: Programa de pós graduação em ciências farmacêuticas, Universidade Federal do Parana, Curitiba, PR, Brasil.
authors.forename: Aguinaldo J
authors.initials: AJ
authors.lastname: Nascimento
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000662:veterinary*; D012423:Rural Health; D014504:Urban Health
keywords: Diagnosis; Dogs; Leishmania infantum; PCR; Serology