title: ENU Mutagenesis in the Mouse.
abstract: This unit describes the treatment of laboratory mice with the mutagen N-ethyl-N-nitrosourea (ENU) to induce very highly increased rates of mutation throughout the genome. Further, it describes several popular mating schemes designed to produce animals displaying phenotypes associated with the induced mutations.
authors.affiliation: Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.
authors.forename: Rolf
authors.initials: R
authors.lastname: Stottmann
authors.affiliation: Center for Developmental Biology and Regenerative Medicine, Seattle Children's Research Institute, Seattle, Washington.
authors.forename: David
authors.initials: D
authors.lastname: Beier
mesh_terms: D000818:Animals; D005038:Ethylnitrosourea / Q000633:toxicity*; D051379:Mice; D016296:Mutagenesis
chemical_list: D005038:Ethylnitrosourea
keywords: alkylating agent; genome sequencing; per locus mutation frequency; phenotype-driven mutagenesis; spermatogonial stem cells