title: Effect of exogenous amylolytic enzymes on the accumulation of chlorogenic acid isomers in wounded potato tubers.
abstract: Potato tubers under wounding stress synthesize chlorogenic acid isomers, which are phenolic compounds that prevent chronic diseases. The biosynthesis of phenolic compounds in plants requires aromatic amino acids that are produced from sugars. Therefore, in this study, we hypothesized that the wound-induced accumulation of chlorogenic acid isomers in potatoes could be enhanced if the availability of sugars is increased by exogenous amylolytic enzymes applied to the surface of the site of wounding. To test this hypothesis, wounded potatoes stored at 20 °C were treated with amylolytic enzymes (pullulanase and amyloglucosidase, 282 units/mL, 10 mL/kg) after being stored for 0 (E0h), 48 (E48h), or 96 h (E96h). The highest level of accumulation of total chlorogenic acid isomers (∼210% higher than that of time 0 h samples) was observed after storage for 120 h for the E96h treatment. The results suggest that increasing the availability of carbon sources needed for the biosynthesis of phenolic compounds would trigger their accumulation in wounded plants.
authors.affiliation: Department of Biotechnology and Food Engineering, Centro de Biotecnología-FEMSA, Tecnológico de Monterrey-Campus Monterrey , E. Garza Sada 2501 Sur, C.P. 64849 Monterrey, NL, México.
authors.forename: Ana Mariel
authors.initials: AM
authors.lastname: Torres-Contreras
authors.forename: Vimal
authors.initials: V
authors.lastname: Nair
authors.forename: Luis
authors.initials: L
authors.lastname: Cisneros-Zevallos
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Jacobo-Velázquez
mesh_terms: D050260:Carbohydrate Metabolism; D002726:Chlorogenic Acid / Q000378:metabolism*; D005087:Glucan 1,4-alpha-Glucosidase / Q000008:administration & dosage / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000008:administration & dosage / Q000378:metabolism*; D007536:Isomerism; D035281:Plant Tubers / Q000378:metabolism*; D011198:Solanum tuberosum / Q000378:metabolism*
chemical_list: D002726:Chlorogenic Acid; D006026:Glycoside Hydrolases; D005087:Glucan 1,4-alpha-Glucosidase; C100181:pullulanase