title: Description of Micrococcus aloeverae sp. nov., an endophytic actinobacterium isolated from Aloe vera.
abstract: A yellow Gram-stain-positive, non-motile, non-endospore -forming, spherical endophytic actinobacterium, designated strain AE-6(T), was isolated from the inner fleshy leaf tissues of Aloe barbadensis (Aloe vera) collected from Pune, Maharashtra, India. Strain AE-6(T) grew at high salt concentrations [10% (w/v) NaCl], temperatures of 15-41 °C and a pH range of 5-12. It showed highest (99.7%) 16S rRNA gene sequence similarity with Micrococcus yunnanensis YIM 65004(T) followed by Micrococcus luteus NCTC 2665(T) (99.6%) and Micrococcus endophyticus YIM 56238(T) (99.0%). Ribosomal protein profiling by MALDI-TOF/MS also showed it was most closely related to M. yunnanensis YIM 65004(T) and M. luteus NCTC 2665(T). Like other members of the genus Micrococcus, strain AE-6(T) had a high content of branched chain fatty acids (iso-C15:0 and anteiso-C15:0). MK-8(H2) and MK-8 were the predominant isoprenoid quinones. Cell wall analysis showed an 'A2 L-Lys-peptide subunit' type of peptidoglycan and ribose to be the major cell wall sugar. The DNA G+C content was 70 mol%. Results of DNA-DNA hybridization of AE-6(T) with its closest relatives from the genus Micrococcus produced a value of less than 70%. Based on the results of this study, strain AE-6(T) could be clearly differentiated from other members of the genus Micrococcus. We propose that it represents a novel species of the genus Micrococcus and suggest the name Micrococcus aloeverae sp. nov., with strain AE-6(T) ( = MCC 2184(T) = DSM 27472(T)) as the type strain of the species.
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Om
authors.initials: O
authors.lastname: Prakash
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Nimonkar
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Hitendra
authors.initials: H
authors.lastname: Munot
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Avinash
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Venkata Ramana
authors.initials: VR
authors.lastname: Vemuluri
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Mahesh S
authors.initials: MS
authors.lastname: Chavadar
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Pune, Maharastra 411007, India.
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
mesh_terms: D000504:Aloe / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007194:India; D008837:Micrococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8