title: Streptomyces zagrosensis sp. nov., isolated from soil.
abstract: The taxonomic position of a novel actinomycete isolated from soil in Fars Province (Iran) was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolate matched those described for members of the genus Streptomyces. On ISP2 medium, strain HM 1154(T) produced a dark cream, branched substrate mycelium and Retinaculiaperti aerial hyphae that in some images also appeared spiral and that developed into greyish-white spore chains with a smooth surface. The isolate showed optimal growth at 28 °C and pH 6-9 with 0-4% (w/v) NaCl. Whole-cell hydrolysates contained ll-diaminopimelic acid as diagnostic diamino acid, ribose and glucose. The main phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, three unknown phospholipids and an unknown aminophospholipid; MK-9(H4) and MK-9(H2) were the predominant menaquinones. The major cellular fatty acids were the branched saturated iso-C16:0 and anteiso-C15:0. Strain HM 1154(T) exhibited the highest 16S rRNA gene sequence similarities to Streptomyces coerulescens DSM 40146(T) (99.4%), Streptomyces varsoviensis DSM 40346(T) (99.3%), Streptomyces youssoufiensis DSM 41920(T) (99.2%), Streptomyces abikoensis DSM 40831(T) (99.2%), Streptomyces rimosus subsp. rimosus DSM 40260(T) (99.1%), Streptomyces luteireticuli DSM 40509(T) (99.1%), Streptomyces thioluteus DSM 40027(T) (99.1%), Streptomyces blastmyceticus DSM 40029(T) (99.0%) and Streptomyces hiroshimensis DSM 40037(T) (99.0%). DNA-DNA hybridization studies showed relatedness values of 11.0-35.8% with the closest related species. Based on these results, strain HM 1154(T) is considered to represent a novel species within the genus Streptomyces, for which the name Streptomyces zagrosensis sp. nov. is proposed. The type strain is HM 1154(T) ( = DSM 42018(T) = UTMC 1154(T) = CECT 8305(T)).
authors.affiliation: University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.|Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455 Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mohammadipanah
authors.affiliation: University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.|Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455 Tehran, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Hamedi
authors.affiliation: Leibniz-Institut DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, 38124 Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: HZI - Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.affiliation: Leibniz-Institut DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, 38124 Braunschweig, Germany.
authors.forename: María Del Carmen
authors.initials: MDC
authors.lastname: Montero-Calasanz
authors.affiliation: Leibniz-Institut DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, 38124 Braunschweig, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007492:Iran; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid