title: Identification and characterization of a short-chain acyl dehydrogenase from Klebsiella pneumoniae and its application for high-level production of L-2,3-butanediol.
abstract: Klebsiella pneumoniae synthesize large amounts of L-2,3-butanediol (L-2,3-BD), but the underlying mechanism has been unknown. In this study, we provide the first identification and characterization of an L-2,3-BD dehydrogenase from K. pneumoniae, demonstrating its reductive activities toward diacetyl and acetoin, and oxidative activity toward L-2,3-BD. Optimum pH, temperature, and kinetics determined for reductive and oxidative reactions support the preferential production of 2,3-BD during cell growth. Synthesis of L-2,3-BD was remarkably enhanced by increasing gene dosage, reaching levels that, to the best of our knowledge, are the highest achieved to date.
authors.affiliation: Biorefinery Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, 580-185, South Korea.
authors.forename: Jang Min
authors.initials: JM
authors.lastname: Park
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Hong
authors.forename: Sung-Mok
authors.initials: SM
authors.lastname: Lee
authors.forename: Sun-Yeon
authors.initials: SY
authors.lastname: Heo
authors.forename: You Ree
authors.initials: YR
authors.lastname: Jung
authors.forename: In Yeong
authors.initials: IY
authors.lastname: Kang
authors.forename: Baek-Rock
authors.initials: BR
authors.lastname: Oh
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000093:Acetoin / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002072:Butylene Glycols / Q000378:metabolism*; D044926:Butyryl-CoA Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D007711:Klebsiella pneumoniae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D002072:Butylene Glycols; C026978:2,3-butylene glycol; D000093:Acetoin; D044926:Butyryl-CoA Dehydrogenase