title: Bovine milk-derived α-lactalbumin inhibits colon inflammation and carcinogenesis in azoxymethane and dextran sodium sulfate-treated mice.
abstract: Cyclooxygenase-2 is expressed early in colon carcinogenesis and plays crucial role in the progress of the disease. Recently, we found that α-lactalbumin had anti-inflammatory activity by inhibiting cyclooxygenase-2. In experiment 1, we investigated the effects of α-lactalbumin on the colon carcinogenesis initiated with azoxymethane (AOM) followed by promotion with dextran sodium sulfate (DSS) in mice. Dietary treatment with α-lactalbumin decreased fecal occult blood score at 3 days after DSS intake. α-Lactalbumin also decreased the colon tumor at week 9. In experiment 2, AOM-treated mice were sacrificed at 7 days after DSS intake. The plasma and colon prostaglandin E2 (PGE2) levels in AOM/DSS-treated mice were higher than those in the DSS-treated mice without initiation by AOM. α-Lactalbumin decreased PGE2 in both plasma and colon. These results suggest that α-lactalbumin effectively inhibited colon carcinogenesis, and the inhibition may be due to the decreased PGE2 by inhibiting cyclooxygenase-2 at cancer promotion stages.
authors.affiliation: a Food Science Research Labs, R&D Division, Meiji Co., Ltd. , Odawara , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Shoko
authors.initials: S
authors.lastname: Takai
authors.forename: Akira
authors.initials: A
authors.lastname: Hosono
authors.forename: Taiichiro
authors.initials: T
authors.lastname: Seki
mesh_terms: D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D000236:Adenoma / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology* / Q000627:therapeutic use; D001397:Azoxymethane / Q000494:pharmacology*; D063646:Carcinogenesis / Q000139:chemically induced / Q000187:drug effects*; D002417:Cattle; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D016264:Dextran Sulfate / Q000494:pharmacology*; D019587:Dietary Supplements; D015232:Dinoprostone / Q000097:blood / Q000378:metabolism; D007249:Inflammation / Q000097:blood / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism; D007768:Lactalbumin / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008892:Milk / Q000737:chemistry*; D009780:Occult Blood; D009929:Organ Size / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D007768:Lactalbumin; D016264:Dextran Sulfate; D015232:Dinoprostone; D001397:Azoxymethane
keywords: colon carcinogenesis; cyclooxygenase; prostaglandin E2; α-lactalbumin