title: The synthesis, self-assembling, and biocompatibility of a novel O-carboxymethyl chitosan cholate decorated with glycyrrhetinic acid.
abstract: O-carboxymethyl chitosan (OCMC) was firstly decorated with cholic acid (CA) to acquire an amphiphilic polymer under alkaline condition. Then glycyrrhetinic acid (GA) was conjugated to the polymer via a succinate linker and finally treated with NaCO3 solution to obtain new conjugates for potential liver targeted delivery. These conjugates formed uniform aggregates with low critical aggregation concentrations (0.028-0.079 mg/mL) in PBS. The average diameter of cholic acid modified carboxymethyl chitosan (CMCA) aggregates (110-257 nm) decreased with the increase of CA substitution degree and became slightly larger after GA modification. Negative zeta potential (-15 mV) of GA decorated CMCA (GA-CMCA) revealed that the formation of negatively charged shells and spherical morphology was observed under transmission electron microscopy. Furthermore, hemolysis test, in vitro cytotoxicity assay and cellular uptake study all demonstrated the safety and feasibility of these conjugates as a promising carrier for liver targeted drug delivery.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Shandong University, 44 Wenhua Xilu, Jinan 250012, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Du
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Shandong University, 44 Wenhua Xilu, Jinan 250012, China.
authors.forename: Xiaoye
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Shandong University, 44 Wenhua Xilu, Jinan 250012, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Pang
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Shandong University, 44 Wenhua Xilu, Jinan 250012, China. Electronic address: professorgxzhai@aliyun.com.
authors.forename: Guangxi
authors.initials: G
authors.lastname: Zhai
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D045744:Cell Line, Tumor; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D020355:Cholates / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D016503:Drug Delivery Systems; D006034:Glycyrrhetinic Acid / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans
chemical_list: D001672:Biocompatible Materials; D020355:Cholates; D004337:Drug Carriers; C504164:O-carboxymethylchitosan; D048271:Chitosan; D006034:Glycyrrhetinic Acid
keywords: Cholic acid; Glycyrrhetinic acid; O-carboxymethylated chitosan; Self-aggregates