title: Enhanced laminin adsorption on nanowires compared to flat surfaces.
abstract: Semiconductor nanowires are widely used to interface living cells, and numerous nanowire-based devices have been developed to manipulate or sense cell behavior. We have, however, little knowledge on the nature of the cell-nanowire interface. Laminin is an extracellular matrix protein promoting cell attachment and growth. Here, we used a method based on fluorescence microscopy and measured the relative amount of laminin adsorbed on nanowires compared to flat surfaces. The amount of adsorbed laminin per surface area is up to 4 times higher on 55nm diameter gallium phosphide nanowires compared to the flat gallium phosphide surface between the nanowires. We show that this enhanced adsorption on nanowires cannot be attributed to electrostatic effects, nor to differences in surface chemistry, but possibly to pure geometrical effects, as increasing the nanowire diameter results in a decreased amount of adsorbed protein. The increased adsorption of laminin on nanowires may explain the exceptionally beneficial properties of nanowire substrates for cellular growth reported in the literature since laminin is often used as surface coating prior to cell cultures in order to promote cell growth, and also because primary cell suspensions contain endogenous laminin.
authors.affiliation: Division of Solid State Physics, Lund University, Lund, Sweden; The Nanometer Structure Consortium, Lund University, Lund, Sweden.
authors.forename: Greger
authors.initials: G
authors.lastname: Hammarin
authors.affiliation: Division of Solid State Physics, Lund University, Lund, Sweden; The Nanometer Structure Consortium, Lund University, Lund, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Persson
authors.affiliation: The Nanometer Structure Consortium, Lund University, Lund, Sweden; Division of Physical Chemistry, Lund University, Lund, Sweden.
authors.forename: Aleksandra P
authors.initials: AP
authors.lastname: Dabkowska
authors.affiliation: Division of Solid State Physics, Lund University, Lund, Sweden; The Nanometer Structure Consortium, Lund University, Lund, Sweden; Neuronano Research Center, Lund University, Lund, Sweden. Electronic address: christelle.prinz@ftf.lth.se.
authors.forename: Christelle N
authors.initials: CN
authors.lastname: Prinz
mesh_terms: D000327:Adsorption; D007797:Laminin / Q000737:chemistry*; D018613:Microscopy, Confocal; D053770:Nanowires; D013499:Surface Properties
chemical_list: D007797:Laminin
keywords: Cell adhesion; Curvature; Laminin; Nanotopography; Nanowires; Protein