title: Lymphoid tissue and plasmacytoid dendritic cells and macrophages do not share a common macrophage-dendritic cell-restricted progenitor.
abstract: The relationship between dendritic cells (DCs) and macrophages is often debated. Here we ask whether steady-state, lymphoid-tissue-resident conventional DCs (cDCs), plasmacytoid DCs (pDCs), and macrophages share a common macrophage-DC-restricted precursor (MDP). Using new clonal culture assays combined with adoptive transfer, we found that MDP fractions isolated by previous strategies are dominated by precursors of macrophages and monocytes, include some multipotent precursors of other hematopoietic lineages, but contain few precursors of resident cDCs and pDCs and no detectable common precursors restricted to these DC types and macrophages. Overall we find no evidence for a common restricted MDP leading to both macrophages and FL-dependent, resident cDCs and pDCs.
authors.affiliation: The Walter and Eliza Hall Institute, Parkville, VIC 3050, Australia; Department of Medical Biology, The University of Melbourne, VIC 3010, Australia. Electronic address: sathe@wehi.edu.au.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Sathe
authors.affiliation: The Walter and Eliza Hall Institute, Parkville, VIC 3050, Australia; Department of Medical Biology, The University of Melbourne, VIC 3010, Australia.
authors.forename: Donald
authors.initials: D
authors.lastname: Metcalf
authors.affiliation: The Walter and Eliza Hall Institute, Parkville, VIC 3050, Australia; Department of Medical Biology, The University of Melbourne, VIC 3010, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Vremec
authors.affiliation: The Walter and Eliza Hall Institute, Parkville, VIC 3050, Australia; Department of Medical Biology, The University of Melbourne, VIC 3010, Australia; The Netherlands Cancer Institute, 1066CX Amsterdam, the Netherlands.
authors.forename: Shalin H
authors.initials: SH
authors.lastname: Naik
authors.affiliation: School of Pathology and Laboratory Medicine, University of Western Australia, WA 6009, Australia.
authors.forename: Wallace Y
authors.initials: WY
authors.lastname: Langdon
authors.affiliation: The Walter and Eliza Hall Institute, Parkville, VIC 3050, Australia; Department of Medical Biology, The University of Melbourne, VIC 3010, Australia.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Huntington
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University School of Medicine, Beijing 100084, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.affiliation: The Walter and Eliza Hall Institute, Parkville, VIC 3050, Australia; Department of Medical Biology, The University of Melbourne, VIC 3010, Australia; Burnet Institute, Melbourne, VIC 3010, Australia. Electronic address: shortman@wehi.edu.au.
authors.forename: Ken
authors.initials: K
authors.lastname: Shortman
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D000073883:CX3C Chemokine Receptor 1; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology; D006098:Granulocytes / Q000166:cytology / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology*; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D055017:Monocyte-Macrophage Precursor Cells / Q000166:cytology* / Q000276:immunology; D009000:Monocytes / Q000166:cytology; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000276:immunology; D019707:Receptors, Chemokine / Q000276:immunology
chemical_list: D000073883:CX3C Chemokine Receptor 1; C471885:Cx3cr1 protein, mouse; D016207:Cytokines; D019707:Receptors, Chemokine; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor