title: A low-cost miniaturized potentiostat for point-of-care diagnosis.
abstract: This paper presents a novel approach of using a miniaturized potentiostat (M-P) chip (LMP91000) to perform full range cyclic voltammetry (CV) measurements for the detection of biomarkers. The LMP91000 evaluation board was reconfigured to perform three-electrode CV measurements in order to achieve electrochemical cortisol immunosensing. The microelectrodes for cortisol estimation were fabricated by immobilizing monoclonal anti-cortisol antibody (Anti-M-Cab) onto self-assembled monolayer (SAM) modified Au microelectrodes. The results obtained using the M-P were compared to those obtained using a conventional potentiostat. The M-P was successful in measuring cortisol levels in the range of pM. The outcomes of the studies suggest that M-P can effectively perform biochemical measurements on three electrode systems, enabling the development of miniature systems for point-of-care (POC) diagnosis.
authors.affiliation: BioMEMS Microsystems Laboratory, Electrical and Computer Engineering, Florida International Laboratory, Miami, FL 33174, USA.
authors.forename: Andres Felipe Diaz
authors.initials: AF
authors.lastname: Cruz
authors.affiliation: BioMEMS Microsystems Laboratory, Electrical and Computer Engineering, Florida International Laboratory, Miami, FL 33174, USA.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Norena
authors.affiliation: Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33199, USA.
authors.forename: Ajeet
authors.initials: A
authors.lastname: Kaushik
authors.affiliation: BioMEMS Microsystems Laboratory, Electrical and Computer Engineering, Florida International Laboratory, Miami, FL 33174, USA. Electronic address: sbhansa@fiu.edu.
authors.forename: Shekhar
authors.initials: S
authors.lastname: Bhansali
mesh_terms: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques; D004867:Equipment Design; D006046:Gold; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis / Q000276:immunology; D008839:Microelectrodes; D019095:Point-of-Care Systems
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D006046:Gold; D006854:Hydrocortisone
keywords: Cortisol; Electrochemical immunosensing; Miniaturized potentiostat; Personalized health monitoring/diagnostic; Point-of-care