title: Serogrouping and pulsed-field gel electrophoresis analysis of Listeria monocytogenes isolates from cases of human infection in Hungary 2004–2012 molecular typing of Listeria monocytogenes in Hungary.
abstract: Eighty isolates of Listeria monocytogenes cultured from human infections in Hungary between 2004 and 2012 were serotyped by the PCR technique of Doumithet al. [9] and characterised by pulsed-field gel electrophoresis (PFGE). Most of the isolates belonged to two serogroups: 53 isolates (66.3%) to serovar group 4b,4d,4e and 21isolates (25.8%) to serogroup 1/2a,3a. Although many pulsotypes were identified a particular pulsotype proved highly excelling comprising of 31 isolates after digestion by both ApaI and AscI restriction enzymes. All strains from this pulsotype belonged toserovar group 4b,4d,4e. Interestingly 24% of isolates from invasive samples(cerebrospinal fluid, blood) belonged to two distinct pulsotypes in the less common serovar group 1/2a,3a. Several small clusters of cases caused by isolates with identical pulsotypes were identified.
authors.forename: Judit
authors.initials: J
authors.lastname: Pászti
authors.forename: Józsefné
authors.initials: J
authors.lastname: Király
authors.forename: Miklós
authors.initials: M
authors.lastname: Füzi
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D008089:Listeria monocytogenes / Q000145:classification* / Q000302:isolation & purification; D008088:Listeriosis / Q000453:epidemiology / Q000382:microbiology; D058889:Molecular Typing; D012703:Serotyping; D013997:Time Factors