title: Human protein kinase CK2 phosphorylates matrix metalloproteinase 2 and inhibits its activity.
abstract: Matrix metalloproteinase 2 (MMP-2) is involved in cancer development and is overexpressed in a variety of malignant tumors. MMP-2 activity is controlled mainly by transcription, proteolytic activation, and inhibition by endogenous inhibitors. It had previously been demonstrated that MMP-2 activity is also regulated by phosphorylation at several sites by protein kinase C. Here we demonstrate, by means of bioinformatics and biochemical and cellular assays, that protein kinase CK2 also acts as a modulator of MMP-2 activity. CK2 down-regulates MMP-2 in vitro, and inhibition of CK2 in human fibrosarcoma cells results in up-regulation of MMP-2. The discovery of the crosstalk between MMP-2 and CK2 opens the possibility of new combined anticancer therapies.
authors.affiliation: Departamento de Química y Bioquímica, Facultad de Farmacia, Universidad CEU San Pablo, Urbanización Monteprincipe, 28668 Madrid (Spain); Department of Molecular Biology, Faculty of Biotechnology and Environment Sciences, The John Paul II Catholic University of Lublin, 20-718 Lublin (Poland).
authors.forename: Kamila
authors.initials: K
authors.lastname: Filipiak
authors.forename: Konrad
authors.initials: K
authors.lastname: Kubiński
authors.forename: Ulf
authors.initials: U
authors.lastname: Hellman
authors.forename: Ana
authors.initials: A
authors.lastname: Ramos
authors.forename: Beatriz
authors.initials: B
authors.lastname: de Pascual-Teresa
mesh_terms: D047390:Casein Kinase II / Q000378:metabolism* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019295:Computational Biology; D015536:Down-Regulation / Q000187:drug effects; D005354:Fibrosarcoma / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology*; D010766:Phosphorylation; D020239:Receptor Cross-Talk / Q000187:drug effects
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D047390:Casein Kinase II; D020778:Matrix Metalloproteinase 2
keywords: MMP-2; cancer; metalloproteins; phosphorylation; protein kinase CK2