title: Altered microRNA expression profiles are involved in resistance to low-dose ionizing radiation in the absence of BMI1 in human dermal fibroblasts.
abstract: The polycomb group RING finger protein, B-cell‑specific moloney murine leukemia virus integration site 1 (BMI1), has emerged as a key regulator of cell proliferation, cell cycle, cell immortalization, chemoresistance and radioresistance. Although the radioresistant effect of BMI1 has been thoroughly investigated, the effectiveness of this factor on low-dose radiation (LDR) resistance has not been explored. Here, we demonstrate that BMI1 is not critical for altering cell viability or cell growth in response to LDR, but BMI1 changes cellular gene expression profiles in response to LDR. Normal human dermal fibroblasts (NHDFs) stably expressing BMI1 short hairpin RNA (shRNA) did not exhibit changes in cell viability or cell cycle distribution assays following exposure to 0.1 Gy of γ-radiation. However, microRNA (miRNA) microarrays revealed that a lack of BMI1 leads to changes in miRNA expression in response to LDR. Bioinformatics analyses demonstrated that predicted target genes of the altered miRNAs are functionally involved in both negative and positive regulation of cell growth, cell proliferation, cell cycle and apoptosis. Therefore, these results indicate that low radiosensitivity even in the absence of the radioresistant factor BMI1 is related with the altered miRNA expression profiles in NHDF.
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Seunghee
authors.initials: S
authors.lastname: Bae
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Karam
authors.initials: K
authors.lastname: Kim
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hwa Jun
authors.initials: HJ
authors.lastname: Cha
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Yeongmin
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Shang Hun
authors.initials: SH
authors.lastname: Shin
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: In-Sook
authors.initials: IS
authors.lastname: An
authors.affiliation: Laboratory of Molecular Oncology, Cheil General Hospital and Women's Healthcare Center, Kwandong University, College of Medicine, Seoul 100-380, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Division of Radiation Cancer Science, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, Republic of Korea.
authors.forename: Jie-Young
authors.initials: JY
authors.lastname: Song
authors.affiliation: Radiation Effect Research Team, Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd., Seoul 132-703, Republic of Korea.
authors.forename: Kwang Hee
authors.initials: KH
authors.lastname: Yang
authors.affiliation: Radiation Effect Research Team, Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd., Seoul 132-703, Republic of Korea.
authors.forename: Seon Young
authors.initials: SY
authors.lastname: Nam
authors.affiliation: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
mesh_terms: D002453:Cell Cycle; D002460:Cell Line; D002470:Cell Survival; D020405:Dermis / Q000166:cytology* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000528:radiation effects*; D005720:Gamma Rays / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000528:radiation effects; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D048054:Mitogen-Activated Protein Kinase 7 / Q000235:genetics / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; C481581:MAPK7 protein, human; D048054:Mitogen-Activated Protein Kinase 7