title: IL-32γ overexpression accelerates streptozotocin (STZ)-induced type 1 diabetes.
abstract: Interleukin-32 (IL-32) is a cytokine produced by T lymphocytes, natural killer (NK) cells, monocytes and epithelial cells. There are five splicing variants (α, β, γ, δ, and ε) and IL-32γ is the most active isoform. We generated human IL-32γ transgenic (IL-32γ TG) mice, displaying a high level of IL-32γ expression in the pancreas. We investigated the effect of IL-32γ on streptozotocin (STZ)-induced type 1 diabetes model using IL-32γ TG mice. After a suboptimal diabetogenic dose of STZ administration, IL-32γ TG mice showed significantly increased blood glucose level comparing with that of wild type (WT) mice at day 5. Inflammatory cytokines levels such as, IL-6, TNFα, IFNγ and IL-1β, in pancreas and liver lysates were accessed by a specific cytokine ELISA. The proinflammatory cytokines were significantly enhanced in the pancreas of IL-32γ TG mice comparing to that of WT mice whereas those cytokines levels in liver of IL-32γ TG and WT mice were not changed by STZ. These data indicate that the overexpression of IL-32γ contributes to initial islet β-cells injury and inflammation in pancreas and aggravates STZ-induced type 1 diabetes.
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hyunjhung
authors.initials: H
authors.lastname: Jhun
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Jida
authors.initials: J
authors.lastname: Choi
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Hong
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Siyoung
authors.initials: S
authors.lastname: Lee
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Areum
authors.initials: A
authors.lastname: Kwak
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Eunsom
authors.initials: E
authors.lastname: Kim
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Seunghyun
authors.initials: S
authors.lastname: Jo
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Soyoon
authors.initials: S
authors.lastname: Ryoo
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Yoojung
authors.initials: Y
authors.lastname: Lim
authors.affiliation: Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Yoon
authors.affiliation: College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: Division of Life Sciences, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Kim
authors.affiliation: Department of Medicine, Pusan Paik Hospital, Collage of Medicine, Inje University, Busan 633-165, Republic of Korea.
authors.forename: Youngmin
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Endocrinology and Metabolism, College of Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Keeho
authors.initials: K
authors.lastname: Song
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: soohyun@konkuk.ac.kr.
authors.forename: Soohyun
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006943:Hyperglycemia / Q000139:chemically induced; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D050417:Insulin-Secreting Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D007371:Interferon-gamma / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D020033:Protein Isoforms / Q000235:genetics; D013311:Streptozocin; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001786:Blood Glucose; C587672:IL1B protein, mouse; C496971:IL32 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D007378:Interleukins; D020033:Protein Isoforms; D014409:Tumor Necrosis Factor-alpha; D013311:Streptozocin; D007371:Interferon-gamma; D005947:Glucose
keywords: Glucose tolerance test; IL-32γ transgenic mice; Inflammatory cytokine; Pancreas; Streptozotocin (STZ)-induced type I diabetes