title: Comparative proteomic analysis reveals growth inhibition by 3-N-alkyloxyestradiol derivative (SERM) in prostate cancer cells.
abstract: OBJECTIVE
  In this study we evaluated the proteomic profile of PC-3 cells treated with novel, 3-N-alkyloxyestradiol derivative, 3-[2-diisopropylamino]-ethoxy-D1,3,5 (10)-estrien-17-one (DI) (USPTO #7,687,486).
  
  
  METHODS
  The growth inhibitory potential of DI was determined by the National Cancer Institute (NCI) Developmental Therapeutics Program. 2-D gel electrophoresis and mass spectrometry were employed to identify differentially expressed proteins after treatment with DI.
  
  
  RESULTS
  Growth inhibitory (GI(50)) results showed that DI inhibited the growth of PC-3 and DU-145 cells, at 13.9 μM and 30.8 μM, respectively. Out of the proteins differentially expressed, five were selected for identification with four of those being successfully identified. The identified proteins play a role in protein folding, cell motility, carbohydrate biosynthesis, and carbohydrate degradation.
  
  
  CONCLUSIONS
  Our studies resulted in the identification of targets associated with the glycolytic pathway and cell motility which have been implicated in the development and progression of many cancers.
authors.affiliation: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, Florida, U.S.A.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Green
authors.affiliation: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, Florida, U.S.A.
authors.forename: John S
authors.initials: JS
authors.lastname: Cooperwood
authors.affiliation: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, Florida, U.S.A.
authors.forename: Equar
authors.initials: E
authors.lastname: Taka
authors.affiliation: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, Florida, U.S.A.
authors.forename: Karam Fa
authors.initials: KF
authors.lastname: Soliman
authors.affiliation: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, Florida, U.S.A.
authors.forename: Carl B
authors.initials: CB
authors.lastname: Goodman
authors.affiliation: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, Florida, U.S.A.
authors.forename: Romonia Renee
authors.initials: RR
authors.lastname: Reams
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000091342:Endoplasmic Reticulum Chaperone BiP; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D020543:Proteome; D040901:Proteomics; D020845:Selective Estrogen Receptor Modulators / Q000494:pharmacology*
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D020543:Proteome; D020845:Selective Estrogen Receptor Modulators; D004958:Estradiol
keywords: GAPDH; GRP78; NCI-60; Prostate cancer; SERMs; proteomics; selective estrogen receptor modulators; triosephosphate isomerase