title: Quantitative response of nitrifying and denitrifying communities to environmental variables in a full-scale membrane bioreactor.
abstract: The abundance and transcription levels of specific gene markers of total bacteria, ammonia-oxidizing Betaproteobacteria, nitrite-oxidizing bacteria (Nitrospira-like) and denitrifiers (N2O-reducers) were analyzed using quantitative PCR (qPCR) and reverse-transcription qPCR during 9 months in a full-scale membrane bioreactor treating urban wastewater. A stable community of N-removal key players was developed; however, the abundance of active populations experienced sharper shifts, demonstrating their fast adaptation to changing conditions. Despite constituting a small percentage of the total bacterial community, the larger abundances of active populations of nitrifiers explained the high N-removal accomplished by the MBR. Multivariate analyses revealed that temperature, accumulation of volatile suspended solids in the sludge, BOD5, NH4(+) concentration and C/N ratio of the wastewater contributed significantly (23-38%) to explain changes in the abundance of nitrifiers and denitrifiers. However, each targeted group showed different responses to shifts in these parameters, evidencing the complexity of the balance among them for successful biological N-removal.
authors.affiliation: Departamento de Microbiología, Facultad de Farmacia, University of Granada, Granada, Spain. Electronic address: cintagomez@ugr.es.
authors.forename: C
authors.initials: C
authors.lastname: Gómez-Silván
authors.affiliation: Microbial Interactions and Processes Research Group, Helmholtz Center for Infection Research, Braunschweig, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Vílchez-Vargas
authors.affiliation: Departamento de Ingeniería Civil, University of Granada, Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Arévalo
authors.affiliation: Departamento de Ingeniería Civil, University of Granada, Granada, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Gómez
authors.affiliation: Departamento de Microbiología, Facultad de Farmacia, University of Granada, Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: González-López
authors.affiliation: Microbial Interactions and Processes Research Group, Helmholtz Center for Infection Research, Braunschweig, Germany.
authors.forename: D H
authors.initials: DH
authors.lastname: Pieper
authors.affiliation: Departamento de Microbiología, Facultad de Farmacia, University of Granada, Granada, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Rodelas
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification / Q000235:genetics; D004777:Environment; D005798:Genes, Bacterial; D057230:Limit of Detection; D008567:Membranes, Artificial; D058465:Nitrification / Q000235:genetics; D016133:Polymerase Chain Reaction; D012621:Seasons; D014158:Transcription, Genetic
chemical_list: D008567:Membranes, Artificial
keywords: AOB; Multivariate analysis; NOB; Reverse-transcription qPCR; Urban wastewater treatment