title: Halovivax limisalsi sp. nov., an extremely halophilic archaeon from hypersaline mud.
abstract: A Gram-stain-negative, cream-pigmented, motile, extremely halophilic archaeon, designated strain IC38(T), was isolated from a saline mud sample taken from a hypersaline lake, Aran-Bidgol, in Iran. The strain required at least 2.5 M NaCl for growth. However, MgCl2 was not required. Optimal growth occurred with 4.3 M NaCl and 0.2 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 35 °C, respectively, and strain IC38(T) was able to grow over a pH range of 6.5-9.0, and a temperature range of 25-45 °C. Analysis of the 16S rRNA gene sequence revealed that strain IC38(T) clustered with the two species of the genus Halovivax, Halovivax asiaticus EJ-46(T) and Halovivax ruber XH-70(T), with sequence similarities of 96.4% and 96.1%, respectively. The similarities between the rpoB' gene of the novel strain and Halovivax asiaticus and Halovivax ruber were 90.7% and 90.3%, respectively. The polar lipid pattern of strain IC38(T) consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. Three unidentified glycolipids and two minor phospholipids were also observed. The DNA G+C content of strain IC38(T) was 62.6 mol%. On the basis of the phylogenetic analysis, as well as the biochemical and physiological characteristics, the new isolate is suggested to be a representative of a novel species of the genus Halovivax, for which the name Halovivax limisalsi sp. nov. is proposed. The type strain of Halovivax limisalsi is IC38(T) ( = IBRC-M 10022(T) = KCTC 4051(T)).
authors.affiliation: Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Amoozegar
authors.affiliation: Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Makhdoumi-Kakhki
authors.affiliation: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.|Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Maliheh
authors.initials: M
authors.lastname: Mehrshad
authors.affiliation: Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Siavash
authors.initials: S
authors.lastname: Riazi
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ventosa
mesh_terms: D001482:Base Composition; D019641:DNA, Archaeal; D019015:Geologic Sediments / Q000382:microbiology*; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007492:Iran; D060106:Lakes / Q000382:microbiology*; D008055:Lipids / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D008055:Lipids; D012336:RNA, Ribosomal, 16S