title: IL-8 promote carcinogenesis of primary epithelial cells from familial adenomatous polyposis.
abstract: Accumulated evidences supported IL-8 play an important role during colorectal carcinogenesis. However, few direct-related evidences are available. In this paper, we found that high level of IL-8 was constitutively present both in familial adenomatous polyposis (FAP) tissue and carcinoma tissue. Using primary epithelial cells from FAP samples, we study IL-8 effect on their growth, migration, and colonies formation. The results showed that IL-8 stimulated cells proliferation, migration, and colonies formation. Furthermore, High level of CEA, CK20, and EGFR was detected after exposure to IL-8 in primary epithelial cells. In conclusion, our findings showed that IL-8 promotes the adenoma-carcinoma transition in primary epithelial cells from FAP. Targeting IL-8 at adenoma stage may prevent or reduce carcinogenesis.
authors.affiliation: Department of Gastroenterology, China-Japan Friendship Hospital, No.2 Yinghua East Road, Beijing, 100029, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Yu
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000378:metabolism / Q000473:pathology*; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000378:metabolism; D063646:Carcinogenesis / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D053554:Keratin-20 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D002272:Carcinoembryonic Antigen; D016209:Interleukin-8; D053554:Keratin-20; D012333:RNA, Messenger; D066246:ErbB Receptors