title: Targeted metabolomic study indicating glycyrrhizin’s protection against acetaminophen-induced liver damage through reversing fatty acid metabolism.
abstract: The present study aimed to give a short report on a possible mechanism of glycyrrhizin to acetaminophen-induced liver toxicity. Seven-day intraperitoneal administration of glycyrrhizin (400 mg/kg/day) to 2- to 3-month-old male C57BL/6N mice (mean weight 27 g) significantly prevents acetaminophen-induced liver damage, as indicated by the activity of alanine transaminase and aspartate aminotransferase. Metabolomics analysis and principal component analysis (PCA) using ultra-fast liquid chromatography coupled to triple time-of-flight mass spectrometer were performed. PCA separated well the control, glycyrrhizin-treated, acetaminophen-treated, and glycyrrhizin+acetaminophen-treated groups. Long-chain acylcarnitines were listed as the top ions that contribute to this good separation, which include oleoylcarnitine, palmitoylcarnitine, palmitoleoylcarnitine, and myristoylcarnitine. The treatment of glycyrrhizin significantly reversed the increased levels of long-chain acylcarnitines induced by acetaminophen administration. In conclusion, this metabolomic study indicates a significant glycyrrhizin protection effect against acetaminophen-induced liver damage through reversing fatty acid metabolism.
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
authors.forename: Yang-Shen
authors.initials: YS
authors.lastname: Jiang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Peng
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Sun
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Dai
authors.forename: Qiu-Ting
authors.initials: QT
authors.lastname: Cao
authors.forename: Ying-Ming
authors.initials: YM
authors.lastname: Sun
authors.forename: Jing-Chun
authors.initials: JC
authors.lastname: Han
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Gao
mesh_terms: D000082:Acetaminophen / Q000633:toxicity*; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002331:Carnitine / Q000031:analogs & derivatives / Q000737:chemistry; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D002853:Chromatography, Liquid; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D013058:Mass Spectrometry; D055442:Metabolome; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000082:Acetaminophen; D019695:Glycyrrhizic Acid; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D002331:Carnitine