title: Alternative splicing of TAF6: downstream transcriptome impacts and upstream RNA splice control elements.
abstract: The TAF6δ pathway of apoptosis can dictate life versus death decisions independently of the status of p53 tumor suppressor. TAF6δ is an inducible pro-apoptotic subunit of the general RNA polymerase II (Pol II) transcription factor TFIID. Alternative splice site choice of TAF6δ has been shown to be a pivotal event in triggering death via the TAF6δ pathway, yet nothing is currently known about the mechanisms that promote TAF6δ splicing. Furthermore the transcriptome impact of the gain of function of TAF6δ versus the loss of function of the major TAF6α splice form remains undefined. Here we employ comparative microarray analysis to show that TAF6δ drives a transcriptome profile distinct from that resulting from depletion of TAF6α. To define the cis-acting RNA elements responsible for TAF6δ alternative splicing we performed a mutational analysis of a TAF6 minigene system. The data point to several new RNA elements that can modulate TAF6δ and also reveal a role for RNA secondary structure in the selection of TAF6δ.
authors.affiliation: RNA Group, Département de microbiologie et d'infectiologie, Faculté de médecine et sciences de la santé, Université de Sherbrooke, and Centre de recherche du CHUS, Pavillon de recherche appliquée sur le cancer, 3201 rue Jean-Migneault, Sherbrooke, Québec, Canada.
authors.forename: Catherine
authors.initials: C
authors.lastname: Kamtchueng
authors.affiliation: RNA Group, Département de microbiologie et d'infectiologie, Faculté de médecine et sciences de la santé, Université de Sherbrooke, and Centre de recherche du CHUS, Pavillon de recherche appliquée sur le cancer, 3201 rue Jean-Migneault, Sherbrooke, Québec, Canada.
authors.forename: Marie-Éve
authors.initials: MÉ
authors.lastname: Stébenne
authors.affiliation: RNA Group, Département de microbiologie et d'infectiologie, Faculté de médecine et sciences de la santé, Université de Sherbrooke, and Centre de recherche du CHUS, Pavillon de recherche appliquée sur le cancer, 3201 rue Jean-Migneault, Sherbrooke, Québec, Canada.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Delannoy
authors.affiliation: RNA Group, Département de microbiologie et d'infectiologie, Faculté de médecine et sciences de la santé, Université de Sherbrooke, and Centre de recherche du CHUS, Pavillon de recherche appliquée sur le cancer, 3201 rue Jean-Migneault, Sherbrooke, Québec, Canada.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Wilhelm
authors.affiliation: Institut des Hautes Etudes Scientifiques, Centre National de la Recherche Scientifique, 35 route de Chartres, Bures sur Yvette, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Léger
authors.affiliation: Institut des Hautes Etudes Scientifiques, Centre National de la Recherche Scientifique, 35 route de Chartres, Bures sur Yvette, France; Université Pierre et Marie Curie, UMR8246 CNRS, 7 quai Saint Bernard, Paris, France.
authors.forename: Arndt G
authors.initials: AG
authors.lastname: Benecke
authors.affiliation: RNA Group, Département de microbiologie et d'infectiologie, Faculté de médecine et sciences de la santé, Université de Sherbrooke, and Centre de recherche du CHUS, Pavillon de recherche appliquée sur le cancer, 3201 rue Jean-Migneault, Sherbrooke, Québec, Canada.
authors.forename: Brendan
authors.initials: B
authors.lastname: Bell
mesh_terms: D017398:Alternative Splicing; D005091:Exons; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D035381:TATA-Binding Protein Associated Factors / Q000235:genetics*; D059467:Transcriptome
chemical_list: D034741:RNA, Small Interfering; C084977:TAF6 protein, human; D035381:TATA-Binding Protein Associated Factors; D012313:RNA