title: Secoiridoid type of antiallergic substances in olive waste materials of three Japanese varieties of Olea europaea.
abstract: 2-Hydroxy-3-ethylidene-5-(methoxycarbonyl)-3,4-dihydro-2H-pyran-4-acetic acid 2-(3,4-dihydroxyphenyl)ethyl ester (3,4-DHPEA-EA) is a kind of secoiridoid first found in three Japanese olive pomaces: Mission, Lucca, and Manzanillo. These varieties showed high activity of 3,4-DHPEA-EA as an antiallergic active substance with IC50 at 33.5 ± 0.6 μg/mL. Because 3,4-DHPEA-EA was the most abundant among the active substances in the pomaces and the activity of 3,4-DHPEA-EA was greater than that of hydroxytyrosol and elenolic acid, 3,4-DHPEA-EA, which has the ester linkage of hydroxytyrosol and elenolic acid, should be essential for antiallergic activity. Although a trace amount (1.04 mg/kg) of luteolin in the pomace showed the highest antiallergic activity with IC50 at 0.752 ± 0.1 μg/mL, we concluded that the entire antiallergic effect derives from the abundance of 3,4-DHPEA-EA, especially in the green olive pomace of the Mission variety in October, which showed the highest level of 3,4-DHPEA-EA (5033 ± 118 mg/kg). Therefore, the Mission variety had the most effective antiallergy property.
authors.affiliation: The United Graduate School of Agricultural Sciences, Ehime University , 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sato
authors.forename: Noboru
authors.initials: N
authors.lastname: Shinozaki
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Tamura
mesh_terms: D018926:Anti-Allergic Agents / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D005511:Food Handling / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D007220:Industrial Waste / Q000032:analysis*; D039823:Iridoids / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D007564:Japan; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D011714:Pyrans / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D012621:Seasons; D013045:Species Specificity
chemical_list: C121925:3,4-dixydroxyphenylethanol elenolic acid; D018926:Anti-Allergic Agents; D007220:Industrial Waste; D039823:Iridoids; D010636:Phenols; D010936:Plant Extracts; D011714:Pyrans