title: A label-free activatable aptamer probe for colorimetric detection of cancer cells based on binding-triggered in situ catalysis of split DNAzyme.
abstract: A novel label-free tailed hairpin-shaped activatable aptamer probe (THAAP) was developed by rationally integrating an aptamer and a split G-quadruplex into one sequence. Based on target recognition-triggered in situ catalysis of split DNAzyme, the THAAP strategy achieved a simple, fast, washing-free, specific and quantitative colorimetric assay of human leukemic CCRF-CEM cells.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Institute of Biology, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, China. kmwang@hnu.edu.cn xiaoxiaohe@hnu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.forename: Duo
authors.initials: D
authors.lastname: Li
authors.forename: Fengzhou
authors.initials: F
authors.lastname: Xu
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: He
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Ye
authors.forename: Jinlu
authors.initials: J
authors.lastname: Tang
authors.forename: Chunmei
authors.initials: C
authors.lastname: He
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D003124:Colorimetry / Q000379:methods; D021881:DNA, Catalytic / Q000378:metabolism*; D054856:G-Quadruplexes; D006801:Humans; D007938:Leukemia / Q000175:diagnosis*
chemical_list: D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic