title: Natural humic substances effects on the life history traits of Latonopsis australis SARS (1888) (Cladocera--Crustacea).
abstract: Cultivation medium is one of the first aspects to be considered in zooplankton laboratory cultivation. The use of artificial media does not concern to reproduce natural conditions to the cultivations, which may be achieved by using natural organic compounds like humic substances (HS). This study aimed to evaluate the effects of a concentrate of dissolved organic carbon (DOC) from the Negro River (NR(1)) and an extraction of humic acids (HA) from humus produced by Eisenia andrei on the life history traits of laboratory-based Latonopsis australis SARS (1888). A cohort life table approach was used to provide information about the effectiveness of NR and HA as supplements for the artificial cultivation of L. australis. Additionally, we seek to observe a maximization of L. australis artificial cultivation fitness by expanding the range of HS concentrations. The first experiment demonstrated that the females of L. australis reared under NR10 (mgDOCL(-1)) may have experienced an acceleration of the population life cycle, as the females have proportionally reproduced more and lived shorter than controls. By contrast, the use of the HA did not improve life history traits considered. The expansion of the concentration range (5, 10, 20 and 50 mgDOCL(-1)) corroborated the patterns observed on the first assay. Results for the fitness estimates combined with shorter lifespans than controls demonstrated trade-offs between reproductive output and female longevity reared under NR conditions, with NR20 been suggested as the best L. australis cultivation medium. This response might be associated with hormone-like effects.
authors.affiliation: Biology Institute - Federal University of Bahia (UFBA), Campus de Ondina, 40170-115 Salvador, Bahia, Brazil. Electronic address: carvalhopereira.tsa@hotmail.com.
authors.forename: Ticiana Soares de Andrade
authors.initials: TS
authors.lastname: de Carvalho-Pereira
authors.affiliation: Biology Institute - Federal University of Bahia (UFBA), Campus de Ondina, 40170-115 Salvador, Bahia, Brazil.
authors.forename: Thirza de Santana
authors.initials: Tde S
authors.lastname: Santos
authors.affiliation: Biology Institute - Federal University of Bahia (UFBA), Campus de Ondina, 40170-115 Salvador, Bahia, Brazil.
authors.forename: Edilene M S
authors.initials: EM
authors.lastname: Pestana
authors.affiliation: Biology Institute - Federal University of Bahia (UFBA), Campus de Ondina, 40170-115 Salvador, Bahia, Brazil.
authors.forename: Fábio Neves
authors.initials: FN
authors.lastname: Souza
authors.affiliation: Biology Institute - Federal University of Bahia (UFBA), Campus de Ondina, 40170-115 Salvador, Bahia, Brazil.
authors.forename: Vivian Marina Gomes Barbosa
authors.initials: VM
authors.lastname: Lage
authors.affiliation: Biology Institute - Federal University of Bahia (UFBA), Campus de Ondina, 40170-115 Salvador, Bahia, Brazil.
authors.forename: Bárbara Janaína Bezerra
authors.initials: BJ
authors.lastname: Nunesmaia
authors.affiliation: Biology Institute - Federal University of Bahia (UFBA), Campus de Ondina, 40170-115 Salvador, Bahia, Brazil.
authors.forename: Palloma Thaís Souza
authors.initials: PT
authors.lastname: Sena
authors.affiliation: Biology Institute - Federal University of Bahia (UFBA), Campus de Ondina, 40170-115 Salvador, Bahia, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Mariano-Neto
authors.affiliation: Biology Institute - Federal University of Bahia (UFBA), Campus de Ondina, 40170-115 Salvador, Bahia, Brazil. Electronic address: marenba@gmail.com.
authors.forename: Eduardo Mendes
authors.initials: EM
authors.lastname: da Silva
mesh_terms: D000818:Animals; D001938:Brazil; D033361:Cladocera / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D005260:Female; D056084:Genetic Fitness; D006812:Humic Substances / Q000633:toxicity*; D011157:Population Dynamics; D045483:Rivers; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D006812:Humic Substances; D014874:Water Pollutants, Chemical
keywords: Artificial cultivation; Dissolved organic carbon; Latonopsis australis; Life history traits; Trade-off