title: Metabolic engineering of Saccharomyces cerevisiae to improve succinic acid production based on metabolic profiling.
abstract: We performed metabolic engineering on the budding yeast Saccharomyces cerevisiae for enhanced production of succinic acid. Aerobic succinic acid production in S. cerevisiae was achieved by disrupting the SDH1 and SDH2 genes, which encode the catalytic subunits of succinic acid dehydrogenase. Increased succinic acid production was achieved by eliminating the ethanol biosynthesis pathways. Metabolic profiling analysis revealed that succinic acid accumulated intracellularly following disruption of the SDH1 and SDH2 genes, which suggests that enhancing the export of intracellular succinic acid outside of cells increases succinic acid production in S. cerevisiae. The mae1 gene encoding the Schizosaccharomyces pombe malic acid transporter was introduced into S. cerevisiae, and as a result, succinic acid production was successfully improved. Metabolic profiling analysis is useful in producing chemicals for metabolic engineering of microorganisms.
authors.affiliation: a Department of Bioinformatic Engineering, Graduate School of Information Science and Technology , Osaka University , Osaka , Japan.
authors.forename: Yuma
authors.initials: Y
authors.lastname: Ito
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirasawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D060847:Metabolic Engineering / Q000379:methods*; D055432:Metabolomics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D019802:Succinic Acid
keywords: Saccharomyces cerevisiae; capillary electrophoresis time-of-flight mass spectrometry; metabolic engineering; metabolic profiling; succinic acid