title: Non-pretreated O-acyl isopeptide of amyloid β peptide 1-42 is monomeric with a random coil structure but starts to aggregate in a concentration-dependent manner.
abstract: An isopeptide of amyloid β peptide 1-42 (isoAβ42) was considered as a non-aggregative precursor molecule for the highly aggregative Aβ42. It has been applied to biological studies after several pretreatments. Here we report that isoAβ42 is monomeric with a random coil structure at 40 μM without any pretreatment. But we also found that isoAβ42 retains a slight aggregative nature, which is significantly weaker than that of the native Aβ42.
authors.affiliation: Peptide Institute, Inc., 7-2-9 Saito-Asagi, Ibaraki-Shi, Osaka 567-0085, Japan. Electronic address: t.yoshiya@peptide.co.jp.
authors.forename: Taku
authors.initials: T
authors.lastname: Yoshiya
authors.affiliation: Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Maruno
authors.affiliation: Peptide Institute, Inc., 7-2-9 Saito-Asagi, Ibaraki-Shi, Osaka 567-0085, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Uemura
authors.affiliation: Peptide Institute, Inc., 7-2-9 Saito-Asagi, Ibaraki-Shi, Osaka 567-0085, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kubo
authors.affiliation: Laboratory of Peptide Science, Nagahama Institute of Bio-Science and Technology, Shiga 526-0829, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kiso
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Sohma
authors.affiliation: Peptide Institute, Inc., 7-2-9 Saito-Asagi, Ibaraki-Shi, Osaka 567-0085, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshizawa-Kumagaye
authors.affiliation: Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Peptide Institute, Inc., 7-2-9 Saito-Asagi, Ibaraki-Shi, Osaka 567-0085, Japan; Graduate School of Science, Osaka University, Osaka 560-0043, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nishiuchi
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)
keywords: Alzheimer’s disease; Amyloid β peptide; Analytical ultracentrifugation; O-Acyl isopeptide; O-to-N intramolecular acyl migration