title: A cost-effective method for preparing, maintaining, and transfecting neurons in organotypic slices.
abstract: The cellular and molecular mechanisms that underlie brain function are challenging to study in the living brain. The development of organotypic slices has provided a welcomed addition to our arsenal of experimental brain preparations by allowing both genetic and prolonged pharmacological manipulations in a system that, much like the acute slice preparation, retains several core features of the cellular and network architecture found in situ. Neurons in organotypic slices can survive in culture for several weeks, can be molecularly manipulated by transfection procedures and their function can be interrogated by traditional cellular electrophysiological or imaging techniques. Here, we describe a cost-effective protocol for the preparation and maintenance of organotypic slices and also describe a protocol for biolistic transfection that can be used to introduce plasmids in a small subset of neurons living in an otherwise molecularly unperturbed network. The implementation of these techniques offers a flexible experimental paradigm that can be used to study a multitude of neuronal mechanisms.
authors.affiliation: Department of Cellular and Molecular Medicine, University of Ottawa, 451 Smyth Road, RGN 3501N, Ottawa, ON, Canada, csoar047@gmail.com.
authors.forename: Cary
authors.initials: C
authors.lastname: Soares
authors.forename: Kevin F H
authors.initials: KF
authors.lastname: Lee
authors.forename: Denise
authors.initials: D
authors.lastname: Cook
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Béïque
mesh_terms: D000818:Animals; D019470:Biolistics / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D001921:Brain / Q000166:cytology / Q000378:metabolism; D004867:Equipment Design; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009924:Organ Culture Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D051381:Rats; D014162:Transfection / Q000191:economics / Q000295:instrumentation / Q000379:methods*