title: [Expression profiling of metastasis-related microRNAs in early esophageal squamous cell carcinoma].
abstract: OBJECTIVE
  To study the difference of microRNA (miRNA) expression between two groups of early stage (pT1N0) esophageal squamous cell carcinoma (ESCC) patients who had different outcome and the prognostic significance of different miRNA in metastatic of early ESCC, and to identify useful prognostic markers in the selection of appropriate treatment for early ESCC patients.
  
  
  METHODS
  TaqMan human miRNA arrays and bioinformatics were used to detect and analyze the expression profiles of miRNAs in the two groups, and RT-PCR was used to verify the differences in miRNA expression.
  
  
  RESULTS
  The miRNA arrays revealed a total of 41 markedly changed miRNAs in the survival group compared with the death group. Bioinformatics analysis, prediction and significant function analyses of targeted genes and pathway analysis identified that miR-27a, miR-143 and miR-886-5p levels were increased or decreased by seven-folds or more. The enriched target genes were GRB2, SOS1, MAPK1, EGFR, CBL, SPRY2, RPS6KA5, IGF1R, NGFR, MAPK14 and CREB1. These genes were significantly related to the following signaling pathways, i.e.Sprouty regulation of tyrosine kinase signals pathway, Erk1/Erk2 Mapk signaling pathway and transcription factor CREB and its extracellular signals.
  
  
  CONCLUSIONS
  miR-27a, miR-886-5p, and miR-143 may be potential prognostic markers of metastasis for early ESCC. The detection of these miRNAs plays a directive role for the treatment options of early ESCC. The regulation of targeted genes and mechanism remain to be further studied.
authors.affiliation: Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Liyan
authors.initials: L
authors.lastname: Xue
authors.forename: Mulan
authors.initials: M
authors.lastname: Jin
authors.affiliation: Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China. E-mail:nlu03@126.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Lyu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000077277:Esophageal Squamous Cell Carcinoma; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000378:metabolism*; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D015398:Signal Transduction
chemical_list: D014408:Biomarkers, Tumor; C542359:MIRN143 microRNA, human; C531388:MIRN27 microRNA, human; C559060:MIRN886 microRNA, human; D035683:MicroRNAs