title: trans-10,cis-12 CLA promotes osteoblastogenesis via SMAD mediated mechanism in bone marrow mesenchymal stem cells.
abstract: The inverse relationship between osteoblast and adipocyte differentiation in bone marrow mesenchymal stem cells has been linked to overall bone mass. It has previously been reported that conjugated linoleic acid (CLA) inhibits adipogenesis via a peroxisome-proliferator activated receptor-γ (PPARγ) mediated mechanism, while it increases osteoblastogenesis via a PPARγ-independent mechanism in mesenchymal stem cells. This suggests potential implication of CLA on improving bone mass. Thus the purpose of this study was to determine involvement of CLA on regulation of osteoblastogenesis in murine mesenchymal stem cells by focusing on the Mothers against decapentaplegic (MAD)-related family of molecules 8 (SMAD8), one of key regulators of osteoblastogenesis. Thetrans-10,cis-12 CLA, but not thecis-9,trans-11, significantly increased osteoblastogenesis via SMAD8, and inhibited adipogenesis independent of SMAD8, while inhibiting factors regulating osteoclastogenesis in this model. These suggest that CLA may help improve osteoblastogenesis via a SMAD8 mediated mechanism.
authors.affiliation: Department of Food Science, University of Massachusetts, Amherst, 102 Holdsworth Way, Amherst, MA 01003.
authors.forename: Jonggun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Food Science, University of Massachusetts, Amherst, 102 Holdsworth Way, Amherst, MA 01003.
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Food Science, University of Massachusetts, Amherst, 102 Holdsworth Way, Amherst, MA 01003.
authors.forename: Yeonhwa
authors.initials: Y
authors.lastname: Park
keywords: CLA; adipogenesis; cis-9, trans-11 CLA; osteoblastogenesis; trans-10,cis-12 CLA