title: Efficient strategies for TALEN-mediated genome editing in mammalian cell lines.
abstract: TALEN is one of the most widely used tools in the field of genome editing. It enables gene integration and gene inactivation in a highly efficient and specific fashion. Although very attractive, the apparent simplicity and high success rate of TALEN could be misleading for novices in the field of gene editing. Depending on the application, specific TALEN designs, activity assessments and screening strategies need to be adopted. Here we report different methods to efficiently perform TALEN-mediated gene integration and inactivation in different mammalian cell systems including induced pluripotent stem cells and delineate experimental examples associated with these approaches.
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France. Electronic address: julien.valton@cellectis.com.
authors.forename: Julien
authors.initials: J
authors.lastname: Valton
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France. Electronic address: jean-pierre.cabaniols@cellectis.com.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Cabaniols
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Romàn
authors.initials: R
authors.lastname: Galetto
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Delacote
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Duhamel
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Paris
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Domique Alain
authors.initials: DA
authors.lastname: Blanchard
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Lebuhotel
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Thomas
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Moriceau
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Raffy
authors.initials: R
authors.lastname: Demirdjian
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Gil
authors.initials: G
authors.lastname: Letort
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Jacquet
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Annabelle
authors.initials: A
authors.lastname: Gariboldi
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Rolland
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Fayza
authors.initials: F
authors.lastname: Daboussi
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Juillerat
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Claudia
authors.initials: C
authors.lastname: Bertonati
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Aymeric
authors.initials: A
authors.lastname: Duclert
authors.affiliation: Cellectis SA, 8 rue de la croix Jarry, 75013 Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Duchateau
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D016678:Genome / Q000235:genetics*; D045325:HCT116 Cells; D006801:Humans; D008969:Molecular Sequence Data; D015533:Transcriptional Activation / Q000235:genetics*; D014162:Transfection / Q000379:methods*
chemical_list: D004268:DNA-Binding Proteins
keywords: Gene editing; Gene inactivation; Gene insertion; Gene tagging; Methylation; TALEN