title: Rice SAPs are responsive to multiple biotic stresses and overexpression of OsSAP1, an A20/AN1 zinc-finger protein, enhances the basal resistance against pathogen infection in tobacco.
abstract: Eukaryotic A20/AN1 zinc-finger proteins (ZFPs) play an important role in the regulation of immune and stress response. After elucidation of the role of first such protein, OsSAP1, in abiotic stress tolerance, 18 rice stress associated protein (SAP) genes have been shown to be regulated by multiple abiotic stresses. In the present study, expression pattern of all the 18 OsSAP genes have been analysed in response to different biotic stress simulators, in order to get insights into their possible involvement in biotic stress tolerance. Our results showed the upregulation of OsSAP1 and OsSAP11 by all biotic stress simulator treatments. Furthermore, the functional role of OsSAP1 in plant defence responses has been explored through overexpression in transgenic plants. Constitutive expression of OsSAP1 in transgenic tobacco resulted into enhanced disease resistance against virulent bacterial pathogen, together with the upregulation of known defence-related genes. Present investigation suggests that rice SAPs are responsive to multiple biotic stresses and OsSAP1 plays a key role in basal resistance against pathogen infection. This strongly supports the involvement of rice SAPs in cross-talk between biotic and abiotic stress signalling pathways, which makes them ideal candidate to design strategies for protecting crop plants against multiple stresses.
authors.affiliation: Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India. Electronic address: himanityagi23@gmail.com.
authors.forename: Himani
authors.initials: H
authors.lastname: Tyagi
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Road, New Delhi 110067, India. Electronic address: sj.bo@jnvu.edu.in.
authors.forename: Shweta
authors.initials: S
authors.lastname: Jha
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Road, New Delhi 110067, India. Electronic address: molikule.sharma@gmail.com.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Sharma
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Road, New Delhi 110067, India. Electronic address: jitender@nipgr.ac.in.
authors.forename: Jitender
authors.initials: J
authors.lastname: Giri
authors.affiliation: Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India; National Institute of Plant Genome Research, Aruna Asaf Ali Road, New Delhi 110067, India. Electronic address: akhilesh@genomeindia.org.
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Tyagi
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D001419:Bacteria; D060467:Disease Resistance / Q000235:genetics*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D015398:Signal Transduction; D013312:Stress, Physiological / Q000235:genetics; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D010940:Plant Proteins
keywords: Biotic stress; Defence signalling; Elicitors; Pseudomonas syringae; Reactive oxygen species (ROS); Stress associated proteins (SAPs)