title: Nondestructive methods for recovery of biological material from human teeth for DNA extraction.
abstract: The extraction of DNA from human skeletal remains applied to forensic, and evolutionary studies do not exclude risks, which are to be evaluated when working with unique specimens that could be damaged or even destroyed. In the present study were evaluated several nondestructive methods for recovering DNA instead of the most currently used pulverization method. Three different procedures to access inside the dental pieces (occlusal perforation, cervical perforation, and cervical cut) have been compared with the aim of recovering as many cell remains as possible to carry out a DNA extraction. Given the DNA quantitation results, a method was proposed that consists of a cervical cut to facilitate the access to the pulp cavity and a subsequent filing of the root canals down to the apex of the dental root. This methodology allows the recovery of both mitochondrial and nuclear DNA, with the minimum deterioration for the dental pieces.
authors.affiliation: Faculty of Science and Technology, Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country UPV/EHU, Barrio Sarriena s/n, Leioa, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Hervella
authors.forename: Maitane G
authors.initials: MG
authors.lastname: Iñiguez
authors.forename: Neskuts
authors.initials: N
authors.lastname: Izagirre
authors.forename: Alberto
authors.initials: A
authors.lastname: Anta
authors.forename: Concepción
authors.initials: C
authors.lastname: de-la-Rúa
mesh_terms: D003481:Cuspid; D016172:DNA Fingerprinting; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D003782:Dental Pulp / Q000737:chemistry; D005553:Forensic Dentistry; D006801:Humans; D008964:Molar, Third; D016133:Polymerase Chain Reaction; D013048:Specimen Handling / Q000379:methods*; D014092:Tooth Root / Q000737:chemistry*
chemical_list: D004272:DNA, Mitochondrial
keywords: DNA recovery; ancient DNA; forensic science; human teeth; mitochondrial DNA; nuclear DNA