title: Isolation and characterization of a novel lectin with mitogenic activity from Pleurotus ferulae.
abstract: Lectins are the tools for the determination of sugar chain structure. Recently, lectin arrays have become a popular new technology; therefore, lectins with specific sugar-binding properties are required. The objective of the study was to isolate a novel lectin from Pleurotus ferulae mushrooms and characterize its various biological activities. A novel lectin was extracted with deionized water, precipitated from the aqueous extract using 75% saturated (NH4)2SO4, and subjected on DEAE-cellulose followed by affinity chromatography on sepharose-6B. The activity was tested using hemagglutination assays, and carbohydrate-binding specificity was determined by glycan microarray analysis. Its effects on the mitogenic activity of mouse splenocytes were determined by MTT assay. The novel lectin was adsorbed on ion-exchange chromatography DEAE-cellulose and shown as a band with the molecular mass of 17.5 kDa on a SDS-PAGE and as a single 35.0-kDa peak in gel filtration on Superdex G-75. The hemagglutinating activity of the lectin was inhibited by D-glucose, lactose, D-galactose, and galactosamine. The lectin was stable on 60°C. The hemagglutinating activity of lectin was reduced by 50% at 70°C. At 80°C, it was further reduced to 6.25% of its original activity. The hemagglutinating activity was the highest at pH 6-9. Moreover, its hemagglutinating activity was inhibited by Mg2+ and Ca2+ ions. The lectin isolated from P. ferulae in the current study possessed highly potent hemagglutinating and proliferative activities toward mouse splenocytes.
authors.affiliation: Food College, Shihezi University, Xinjiang, P.R. China.
authors.forename: Cheng-Jian
authors.initials: CJ
authors.lastname: Xu
authors.affiliation: Department of Geriatrics, the First Affiliated Hospital of Shihezi University, Xinjiang, P.R. China.
authors.forename: Yue-Xiang
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Food College, Shihezi University, Xinjiang, P.R. China.
authors.forename: Bo-Nan
authors.initials: BN
authors.lastname: Niu
authors.affiliation: Food College, Shihezi University, Xinjiang, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Food College, Shihezi University, Xinjiang, P.R. China.
authors.forename: Ying-Biao
authors.initials: YB
authors.lastname: Li
authors.affiliation: Food College, Shihezi University, Xinjiang, P.R. China.
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Food College, Shihezi University, Xinjiang, P.R. China.
authors.forename: Shi-Ling
authors.initials: SL
authors.lastname: Lu
mesh_terms: D000818:Animals; D005260:Female; D006386:Hemagglutination Tests; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D020076:Pleurotus / Q000737:chemistry*
chemical_list: D037102:Lectins