title: Application of 3.0 tesla magnetic resonance imaging for diagnosis in the orthotopic nude mouse model of pancreatic cancer.
abstract: The aim of this study was to successfully establish an orthotopic murine model using two different human pancreatic adenocarcinoma cell lines and to propose a 3.0 tesla MRI protocol for noninvasive characterization of this model. SW1990 and MIAPaca-2 tumor cells were injected into the pancreas of BALB/C nu/nu mice. Tumor growth rate and morphological information were assessed by 3.0 tesla MRI (T1WI, T2WI and DCE-MRI) and immunohistology. Proliferation of SW1990 was significantly faster than that of MIAPaca-2 (P=0.000), but MIAPaca-2 mice had a significantly shorter survival than SW1990 mice (41 days and 44 days respectively, P=0.027). MRI could reliably monitor tumor growth in both cell lines: the tumors exhibiting a spherical growth pattern showed a high-intensity signal, and the SW1990 group developed significantly larger tumors compared with the MIAPaCa-2 group. There were no statistical differences between the two groups in which tumor size was assessed using electronic calipers and an MRI scan (P=0.680). Both tumors showed a slow gradual enhancement pattern. Immunohistochemistry demonstrated tumor tissues showing high expression of Ki-67. This model closely mimics human pancreatic cancer and permits monitoring of tumor growth and morphological information by noninvasive 3.0 tesla MRI studies reducing the number of mice required.
authors.affiliation: Department of Intervetional Radiology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 20032, P.R.China.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Xiuzhong
authors.initials: X
authors.lastname: Yao
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Xu
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhang
authors.forename: Caixia
authors.initials: C
authors.lastname: Fu
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology
chemical_list: D019394:Ki-67 Antigen; C498355:Mki67 protein, mouse