title: Association study of a common genetic variant in pre-miR-1596 with chicken performance traits.
abstract: Increasing reports have verified that miRNAs had an important effect on the growth and development in farm animals. To evaluate the possible effect of miR-1596 polymorphisms on chicken economic traits, directly sequencing and polymerase chain reaction-restriction fragment length polymorphism, association analysis as well as online software were used. The results showed that a C > T polymorphism existed in the miR-1596 gene of the Gushi × Anka F2 resource population. The association analysis showed that it was significantly relevant with the potential of hydrogen of leg muscle, fat content of dry sample and fat content of fresh sample, shank length at 0 day and 4 weeks of age; leg weight, leg muscle weight, and breast muscle weight (P < 0.05); and highly significant association with shank girth at 8 weeks of age and abdominal fat weight (P < 0.01). We predicted the secondary structure of Gallus gallus-miR-1596 (gga-miR-1596) and the free energy by using M-fold, which were not altered. MiR-1596 is conserved between chicken and turkey. Our data implied that miR-1596 might participate in regulating the muscle development and adipogenesis.
authors.affiliation: Henan Innovative Engineering Research Center of Poultry Germplasm Resource, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Yadong
authors.initials: Y
authors.lastname: Tian
authors.forename: Guirong
authors.initials: G
authors.lastname: Sun
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Liu
authors.forename: Ruirui
authors.initials: R
authors.lastname: Jiang
authors.forename: Ruili
authors.initials: R
authors.lastname: Han
authors.forename: Guoxi
authors.initials: G
authors.lastname: Li
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Kang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000254:growth & development*; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D056726:Genetic Association Studies; D016014:Linear Models; D008460:Meat; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D035683:MicroRNAs