title: Serratia odorifera mediated enhancement in susceptibility of Aedes aegypti for chikungunya virus.
abstract: OBJECTIVE
  The susceptibility of the mosquito to the invading pathogen is predominantly dictated by the complex interactions between the mosquito midgut and the surface proteins of the invading pathogen. It is well documented that the midgut microbiota plays an important role in determining the susceptibility of the mosquito to the pathogen. In the present study, we investigated the influence of Serratia odorifera, an endogenous cultivable midgut inhabitant of Aedes aegypti on the chikungunya virus (CHIKV) susceptibility to this mosquito.
  
  
  METHODS
  Ae. aegypti females free of gutflora were co-fed with CHIKV and either of the two midgut inhabitants namely, S. odorifeara and Microbacterium oxydans. CHIKV dissemination was checked on 10 th day post feeding (DPF) using indirect immunoflurescence assay and plaque assay. CHIKV interacting proteins of the mosquito midgut were identified using virus overlay protein binding assay and MALDI TOF/TOF analysis.
  
  
  RESULTS
  The observations revealed that co-feeding of S. odorifera with CHIKV significantly enhanced the CHIKV susceptibility in adult Ae. aegypti, as compared to the mosquitoes fed with CHIKV alone and CHIKV co-fed with another midgut inhabitant, M. oxydans. Virus overlay protein binding assay (VOPBA) results revealed that porin and heat shock protein (HSP60) of Ae. aegypti midgut brush border membrane fraction interacted with CHIKV.
  
  
  CONCLUSIONS
  The results of this study indicated that the enhancement in the CHIKV susceptibility of Ae. aegypti females was due to the suppression of immune response of Ae. aegypti as a result of the interaction between S. odorifera P40 protein and porin on the gut membrane.
authors.forename: Anjali D
authors.initials: AD
authors.lastname: Apte-Deshpande
authors.forename: Mandar S
authors.initials: MS
authors.lastname: Paingankar
authors.forename: Mangesh D
authors.initials: MD
authors.lastname: Gokhale
authors.affiliation: Department of Zoology, Centre for Advance Studies, University of Pune, Pune, India.
authors.forename: Dileep N
authors.initials: DN
authors.lastname: Deobagkar
mesh_terms: D000330:Aedes / Q000382:microbiology / Q000821:virology; D000818:Animals; D018834:Chaperonin 60 / Q000378:metabolism; D065632:Chikungunya Fever / Q000473:pathology / Q000635:transmission* / Q000821:virology; D002646:Chikungunya virus / Q000254:growth & development / Q000472:pathogenicity*; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology / Q000821:virology; D006801:Humans; D007303:Insect Vectors / Q000382:microbiology / Q000821:virology; D051379:Mice; D012705:Serratia / Q000254:growth & development / Q000472:pathogenicity*
chemical_list: D018834:Chaperonin 60