title: Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico.
abstract: A rapid protocol for polar lipid profiling was applied to Euglena gracilis lipid metabolism by LipidBlast, an MS/MS spectral similarity search tool. The similarity search results suggested anoxia-induced polar lipid metabolism in Euglena characterized by the accumulation of differential lipid classes, carbon chain lengths, and unsaturated bond numbers. The informatics-supported MS spectral search provides an alternative option for global lipid profiling studies.
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Sakai , Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Furuhashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Okazawa
authors.forename: Rai
authors.initials: R
authors.lastname: Nakai
authors.forename: Masami
authors.initials: M
authors.lastname: Nakazawa
authors.forename: Tobias
authors.initials: T
authors.lastname: Kind
authors.forename: Oliver
authors.initials: O
authors.lastname: Fiehn
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Kanaya
authors.forename: Masanori
authors.initials: M
authors.lastname: Arita
authors.forename: Daisaku
authors.initials: D
authors.lastname: Ohta
mesh_terms: D000693:Anaerobiosis; D003198:Computer Simulation; D005056:Euglena gracilis / Q000254:growth & development / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D008055:Lipids
keywords: Euglena gracilis; LC–MS/MS; LipidBlast; polar lipid profiling