title: Click synthesis of glucose-functionalized hydrophilic magnetic mesoporous nanoparticles for highly selective enrichment of glycopeptides and glycans.
abstract: Selective enrichment of glycopeptides from complex biological samples is essential for MS-based glycoproteomics, but challenges still remain. In this work, glucose-functionalized magnetic mesoporous nanoparticles (MMNs), which hold the attractive features of well-defined core/shell structure, high specific surface area (324m(2)/g), narrow pore size distribution (2.2nm) and high magnetic responsivity (69.1emug(-1)), were synthesized via click chemistry and applied to enrich glycopeptides and glycans. Taking advantages of the size-exclusive effect of mesopore against proteins and the hydrophilic interaction between glycans and glucose, the hydrophilic MMNs possessed high selectivity for glycopeptides at the digested mixtures of horseradish peroxidase (HRP), myoglobin and β-casein at molar ratio of 1:1:10, large enrichment capacity (as high as 250mg/g), high sensitivity (50fmol), excellent speed (5min for enrichment) and high recovery of glycopeptides (as high as 94.6%). Additionally, the MMNs exhibited excellent performance in enrichment of N-linked glycans from the digested human serum that are made up of peptides, large proteins and other compounds. These outstanding features will give the hydrophilic MMNs high benefit for MS analysis of low-abundance glycopeptides/glycans.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address: zianlin@fzu.edu.cn.
authors.forename: Zian
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Huanghao
authors.initials: H
authors.lastname: Yang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address: zlan@fzu.edu.cn.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification; D057930:Click Chemistry; D005947:Glucose / Q000737:chemistry*; D006020:Glycopeptides / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D060328:Magnetic Phenomena; D008969:Molecular Sequence Data; D009211:Myoglobin / Q000737:chemistry / Q000302:isolation & purification; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D016062:Porosity
chemical_list: D002364:Caseins; D006020:Glycopeptides; D009211:Myoglobin; D010446:Peptide Fragments; D011134:Polysaccharides; D006735:Horseradish Peroxidase; D005947:Glucose
keywords: Click chemistry; Enrichment; Glycan; Glycopeptide; Hydrophilic; Magnetic mesoporous nanoparticles