title: The microbial cell-functional unit for energy dependent multistep biocatalysis.
abstract: Whole-cell biocatalysis has emerged as an important tool for the synthesis of value-added fine and bulk chemicals as well as pharmaceuticals. Especially, the rapid development of recombinant DNA technologies resulted in a shift from the exploitation of natural enzymes and pathways to the design of recombinant cell factories comprising heterologous enzymes and/or synthetic, orthologous pathways for the synthesis of industrially relevant compounds. This review discusses recent developments and concepts applied in the frame of multistep whole-cell biocatalysis along with representative examples.
authors.affiliation: Laboratory of Chemical Biotechnology, Faculty of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Straße 66, 44227 Dortmund, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Ladkau
authors.affiliation: Laboratory of Chemical Biotechnology, Faculty of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Straße 66, 44227 Dortmund, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmid
authors.affiliation: Laboratory of Chemical Biotechnology, Faculty of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Straße 66, 44227 Dortmund, Germany. Electronic address: Bruno.Buehler@bci.tu-dortmund.de.
authors.forename: Bruno
authors.initials: B
authors.lastname: Bühler
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D004734:Energy Metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins