title: Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds coated with PhaP-RGD fusion protein promotes the proliferation and chondrogenic differentiation of human umbilical cord mesenchymal stem cells in vitro.
abstract: Human umbilical cord blood-derived mesenchymal stem cells (hUC-MSCs) have been widely used in tissue engineering. The aim of this study is to evaluate the ability of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) scaffolds coated with polyhydroxyalkanoate binding protein fused with arginyl-glycyl-aspartic acid (PhaP-RGD) to promote the proliferation and chondrogenic differentiation of hUC-MSCs seeded on them. The PhaP-RGD fusion protein was expressed by Escherichia coli. PHBHHx films were coated with PhaP-RGD fusion protein and the physiochemical properties were examined. hUC-MSCs were seeded on PHBHHx films with or without PhaP-RGD precoating and tested for changes in morphology, viability, and chondrogenic differentiation. We found that PhaP-RGD-coated PHBHHx films had similar surface morphology to uncoated PHBHHx. The water contact angle of the coated PHBHHx surface was lower than that of the uncoated surface (10.63° vs. 98.69°). At 7 and 14 days after seeding, the PhaP-RGD-coated PHBHHx group showed greater numbers of viable cells compared to the uncoated PHBHHx group. The expression levels of aggrecan and collagen II were enhanced in the PhaP-RGD-coated PHBHHx group relative to the uncoated PHBHHx group. Histological analysis using toluidine blue staining showed elevated formation of proteoglycan producing chondrocytes in the PhaP-RGD-coated PHBHHx group. Additionally, the synthesis of proteoglycan and collagen was significantly enhanced within the PhaP-RGD constructs. Taken together, PhaP-RGD coating promotes the proliferation and chondrogenic differentiation of hUC-MSCs seeded on PHBHHx films. PhaP-RGD-coated PHBHHx may be a useful scaffold for cartilage tissue engineering.
authors.affiliation: Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Li
authors.forename: Huimin
authors.initials: H
authors.lastname: Chang
authors.forename: Huanan
authors.initials: H
authors.lastname: Luo
authors.forename: Zhenghui
authors.initials: Z
authors.lastname: Wang
authors.forename: Guoxi
authors.initials: G
authors.lastname: Zheng
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Lu
authors.forename: Xijing
authors.initials: X
authors.lastname: He
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Liang
authors.forename: Min
authors.initials: M
authors.lastname: Xu
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D002208:Caproates / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020219:Chondrogenesis; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry*; D011993:Recombinant Fusion Proteins; D054457:Tissue Scaffolds / Q000737:chemistry*; D014470:Umbilical Cord / Q000166:cytology / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D001426:Bacterial Proteins; D002208:Caproates; D020099:Coated Materials, Biocompatible; D004268:DNA-Binding Proteins; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; C118828:PHAP protein, Bacteria; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C047981:arginyl-glycyl-aspartic acid
keywords: cartilage; polyhydroxyalkanoate; scaffold; surface modification; tissue engineering