title: Construction and significance analysis of the MicroRNA expression profile of Hemerocallis fulva at low temperature.
abstract: MicroRNA (miRNA) identification was performed in Hemerocallis fulva by high-throughput sequencing in combination with bioinformatics prediction. A total of 14,843,184 and 16,072,575 RNA sequences were explored under normal and low temperature conditions, respectively. There was a significant difference in RNAs species and quantity between the two samples. Of all the miRNAs, 26 were significantly upregulated and 30 were significantly downregulated, while nine were either significantly upregulated or downregulated under low-temperature stress. Twenty-one highly expressed miRNA families were screened in at least six species. The number of miRNA families was very similar between monocotyledons and dicotyledons, and only a few were more frequently found in monocotyledons.
authors.affiliation: a College of Life Science , Northeast Agricultural University , Harbin , China.
authors.forename: Fengxia
authors.initials: F
authors.lastname: An
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Jingfu
authors.initials: J
authors.lastname: Li
authors.forename: Xiuling
authors.initials: X
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Han
authors.forename: Fuheng
authors.initials: F
authors.lastname: Li
mesh_terms: D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D031421:Hemerocallis / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
keywords: Hemerocallis fulva; environmental stress; gene expression profile; high-throughput sequencing; miRNA