title: Bio-specific and bio-orthogonal chemistries to switch-off the quencher of a FRET-based fluorescent probe: application to living-cell biothiol imaging.
abstract: We report the first molecular system that is responsive to both a bio-specific and a bio-orthogonal stimulus. This dual activation process was applied to the design of a biothiol-specific FRET-based fluorescent probe that could be turned-on via an original concept of quencher bleaching.
authors.affiliation: Laboratory of Functional Chemo Systems, UMR 7199, CNRS-UdS, LABEX MEDALIS, UMR 7199 UdS/CNRS, Faculty of Pharmacy, 74 Route du Rhin, 67401 Illkirch-Graffenstaden, France. alwag@unistra.fr.
authors.forename: C
authors.initials: C
authors.lastname: Egloff
authors.forename: S A
authors.initials: SA
authors.lastname: Jacques
authors.forename: M
authors.initials: M
authors.lastname: Nothisen
authors.forename: D
authors.initials: D
authors.lastname: Weltin
authors.forename: C
authors.initials: C
authors.lastname: Calligaro
authors.forename: M
authors.initials: M
authors.lastname: Mosser
authors.forename: J-S
authors.initials: JS
authors.lastname: Remy
authors.forename: A
authors.initials: A
authors.lastname: Wagner
mesh_terms: D016475:3T3 Cells; D000818:Animals; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds