title: Integration of platinum nanoparticles with a volumetric bar-chart chip for biomarker assays.
abstract: Platinum nanoparticles (PtNPs) efficiently catalyze the transformation of H2 O2 into oxygen gas. However, owing to the lack of an efficient approach or device that can measure the produced oxygen gas, the catalytic reaction has never been used for diagnostic applications. Microfluidics technology provides a platform that meets these requirements. The volumetric bar-chart chip (V-Chip) volumetrically measures the production of oxygen gas by PtNPs and can be integrated with ELISA technology to provide visible and quantitative readouts without expensive instrumentation or complicated data processing. Herein we show that PtNPs outperform catalase with respect to stability at high H2 O2 concentrations or temperatures or in long-term reactions, and are resistant to most catalase inhibitors. We also show that the catalase-like activity of PtNPs can be used in combination with the V-Chip to sensitively and specifically detect cancer biomarkers both in serum and on the cell surface.
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, Department of Pathology and Genomic Medicine, Houston Methodist Hospital, Department of Cell and Developmental Biology, Weill Medical College of Cornell University, 6670 Bertener Avenue, Houston, TX 77030 (USA).
authors.forename: Yujun
authors.initials: Y
authors.lastname: Song
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Xia
authors.forename: Xifeng
authors.initials: X
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Lidong
authors.initials: L
authors.lastname: Qin
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood; D045744:Cell Line, Tumor; D004867:Equipment Design; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009369:Neoplasms / Q000175:diagnosis*; D010984:Platinum / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D010984:Platinum; D006861:Hydrogen Peroxide
keywords: biomarker assays; catalytic activity; immunoassays; microfluidics; platinum nanoparticles