title: Effects of topical application of patchouli alcohol on the UV-induced skin photoaging in mice.
abstract: Ultraviolet (UV) irradiation, known to generate reactive oxygen species (ROS) excessively and elicit inflammatory response, is a potent inducer for skin photoaging. Overproduction of ROS in conjunction with the resulting inflammation stimulate the over-expression of matrix metalloproteinases (MMPs), which in turn causes degradation of extracellular matrix, leading finally to coarse wrinkling, dryness, and laxity of the skin. In this study, patchouli alcohol (PA, C15H26O), an active chemical ingredient reputed for free radical scavenging and anti-inflammatory properties, was investigated for its anti-photoaging action using a mouse model whose dorsal skin was depilated. The dorsal skin areas of six-week-old mice were smeared with PA solution or vehicle, followed by UV irradiation for nine consecutive weeks. Protective effects of PA were evaluated macroscopically and histologically, as well as by assaying the antioxidant enzymes (SOD, GSH-Px) activities, the contents of inflammatory factors (IL-10, IL-6, TNF-α), and the levels of MMP-1 and MMP-3. Our findings amply demonstrated that PA significantly accelerated the recovery of the UV-induced skin lesions, evidently through anti-oxidant and anti-inflammatory action, as well as down-regulation of the MMP-1 and MMP-3 expression.
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.
authors.forename: Xue-Xuan
authors.initials: XX
authors.lastname: Feng
authors.affiliation: The First Affiliated Hospital of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.
authors.forename: Xiu-Ting
authors.initials: XT
authors.lastname: Yu
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Li
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.
authors.forename: Song-Zhi
authors.initials: SZ
authors.lastname: Kong
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Liu
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.
authors.forename: Xie
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong Special Administrative Region.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Xian
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China. Electronic address: suziren@gzucm.edu.cn.
authors.forename: Zi-Ren
authors.initials: ZR
authors.lastname: Su
authors.affiliation: School of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong Special Administrative Region. Electronic address: linzx@cuhk.edu.hk.
authors.forename: Zhi-Xiu
authors.initials: ZX
authors.lastname: Lin
mesh_terms: D000287:Administration, Topical; D000818:Animals; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D012717:Sesquiterpenes / Q000008:administration & dosage* / Q000494:pharmacology*; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects*; D012882:Skin Tests; D014466:Ultraviolet Rays
chemical_list: D012717:Sesquiterpenes; C052983:patchouli alcohol
keywords: Anti-inflammation; Anti-oxidant; Matrix metalloproteinases; Patchouli alcohol; Skin photoaging; Ultraviolet