title: The serum level of transforming growth factor beta1 and its association with Foxp3 gene polymorphism in Iranian women with recurrent spontaneous abortion.
abstract: The aim of the study was to determine the role of transforming growth factor-beta1 (TGF-β1) and Foxp3 (rs3761548) promoter polymorphisms in Iranian women with recurrent spontaneous abortion (RSA). Eighty women with RSA were compared with eighty in a control group. Serum levels of TGF-β1 were measured using ELISA and Foxp3 (rs3761548) promoter polymorphisms using a PCR-RFLP technique. In addition, serum levels of TGF-β1 were compared in different genotypes in the two groups. The women's ages in the two groups were similar (30.15 ± 4.42 years [RSA] vs. 29.97 ± 4.51 [control]) as were serum TGF-β1 concentrations in case and control groups (53.42 ± 2.08 ng/ml in control and 56.31 ± 2.58 ng/ml in the RSA group; p = 0.4). Furthermore, there was no significant difference in the genotype frequencies of the rs3761548 Foxp3 gene between the two groups (p = 0.3) and the levels of TGF-β1 were similar in different genotypes. In conclusion, the data indicate that serum TGF-β1 levels and Foxp3 (rs3761548) promoter polymorphism is not a risk factor for RSA and that there is no association between the polymorphism and serum TGF-β1 levels.
authors.affiliation: Department of Immunology, Reproductive Immunology Research Center, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences , Yazd , Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Hadinedoushan
authors.forename: Narjes
authors.initials: N
authors.lastname: Abbasirad
authors.forename: Abbas
authors.initials: A
authors.lastname: Aflatoonian
authors.forename: Gilda
authors.initials: G
authors.lastname: Eslami
mesh_terms: D000026:Abortion, Habitual / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D056726:Genetic Association Studies; D006801:Humans; D007492:Iran; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D053773:Transforming Growth Factor beta1 / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1
keywords: Foxp3 gene; T regulatory; TGF-β1; polymorphism; recurrent abortion