title: Anti-Inflammatory Spirostanol and Furostanol Saponins from Solanum macaonense.
abstract: Eight new spirostanol saponins, macaosides A-H (1-8), and 10 new furostanol saponins, macaosides I-R (9-18), together with six known spirostanol compounds (19-24) were isolated from Solanum macaonense. The structures of the new compounds were determined from their spectroscopic data, and the compounds were tested for in vitro antineutrophilic inflammatory activity. It was found that both immediate inflammation responses including superoxide anion generation and elastase release were significantly inhibited by treatment with compounds 20, 21, and 24 (superoxide anion generation: IC50 7.0, 7.6, 4.0 μM; elastase release: IC50 3.7, 4.4, 1.0 μM, respectively). However, compounds 1 and 4 exhibited effects on the inhibition of elastase release only, with IC50 values of 3.2 and 4.2 μM, respectively, while 19 was active against superoxide anion generation only, with an IC50 value of 6.1 μM. Accordingly, spirostanols may be promising lead compounds for further neutrophilic inflammatory disease studies.
authors.affiliation: School of Pharmacy, College of Pharmacy, China Medical University , Taichung 40402, Taiwan.|Chinese Medicine Research and Development Center, China Medical University Hospital , Taichung 40447, Taiwan.
authors.forename: Chia-Lin
authors.initials: CL
authors.lastname: Lee
authors.affiliation: Graduate Institute of Natural Products, College of Medicine, and Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University , Taoyuan 33302, Taiwan.
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.affiliation: School of Pharmacy, College of Pharmacy, China Medical University , Taichung 40402, Taiwan.|Chinese Medicine Research and Development Center, China Medical University Hospital , Taichung 40447, Taiwan.
authors.forename: Juan-Cheng
authors.initials: JC
authors.lastname: Yang
authors.affiliation: Chinese Medicine Research and Development Center, China Medical University Hospital , Taichung 40447, Taiwan.
authors.forename: Hao-Ting
authors.initials: HT
authors.lastname: Cheng
authors.affiliation: Chinese Medicine Research and Development Center, China Medical University Hospital , Taichung 40447, Taiwan.
authors.forename: Wan-Jung
authors.initials: WJ
authors.lastname: He
authors.affiliation: Graduate Institute of Natural Products, Kaohsiung Medical University , Kaohsiung 80708, Taiwan.
authors.forename: Chiao-Ting
authors.initials: CT
authors.lastname: Yen
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University , Taichung 40402, Taiwan.
authors.forename: Chao-Lin
authors.initials: CL
authors.lastname: Kuo
authors.affiliation: Graduate Institute of Integrated Medicine, China Medical University , Taichung 40402, Taiwan.|Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital , Taichung 40447, Taiwan.
authors.forename: Chao-Jung
authors.initials: CJ
authors.lastname: Chen
authors.affiliation: Graduate Institute of Natural Products, College of Medicine, and Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University , Taoyuan 33302, Taiwan.|Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology , Taoyuan 33303, Taiwan.
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Chang
authors.affiliation: School of Pharmacy, College of Pharmacy, China Medical University , Taichung 40402, Taiwan.|Chinese Medicine Research and Development Center, China Medical University Hospital , Taichung 40447, Taiwan.|Graduate Institute of Natural Products, Kaohsiung Medical University , Kaohsiung 80708, Taiwan.
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010196:Pancreatic Elastase / Q000378:metabolism; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D032322:Solanum / Q000737:chemistry*; D013150:Spirostans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013624:Taiwan
chemical_list: D000893:Anti-Inflammatory Agents; D012503:Saponins; D013150:Spirostans; D010196:Pancreatic Elastase