title: Characterization of industrially-valuable xylano-pectinolytic enzymes produced concurrently by a novel isolate of Bacillus pumilus.
abstract: Xylanase and polygalacturonase were concurrently produced by a novel alkalo-thermotolerant Bacillus pumilus AJK. They were purified and characterized to evaluate their potential for various industrial applications. Xylanase was purified to 19-fold with 67 % recovery and polygalacturonase up to 23-fold with 75 % recovery. Existence of multiple forms of xylanase was indicated by its elution-profile through Sephadex G-100 as two peaks, xylanase-I and xylanase-II, with molecular weights of ~24.5 and ~13 kDa, respectively, and by the presence of two pH optima, one at pH 6.0 and other at pH 8.5. The molecular weight of polygalacturonase was ~40 kDa by gel-filtration chromatography. Zymographic studies confirmed the presence of seven isozymic forms of xylanase. Xylanase and polygalacturonase are stable over a broad range of pH and temperature.
authors.affiliation: Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India, amanjotbiotech@gmail.com.
authors.forename: Amanjot
authors.initials: A
authors.lastname: Kaur
authors.forename: Avtar
authors.initials: A
authors.lastname: Singh
authors.forename: Ritu
authors.initials: R
authors.lastname: Mahajan
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008670:Metals; D008970:Molecular Weight; D011096:Polygalacturonase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D008670:Metals; D011096:Polygalacturonase; D043364:Endo-1,4-beta Xylanases