title: Aberrant promoter methylation of the cadherin 13 gene in serum and its relationship with clinicopathological features of prostate cancer.
abstract: OBJECTIVE
  To investigate the clinical significance of cadherin 13 (CDH13) gene promoter methylation in the serum of patients with prostate cancer.
  
  
  METHODS
  This prospective study examined the methylation status of CDH13 in serum samples obtained from patients with primary prostate cancer and age-matched control subjects, using methylation-specific polymerase chain reaction. Associations between methylation status of CDH13 and various clinicopathological features and patient survival were evaluated.
  
  
  RESULTS
  A total of 98 patients with prostate cancer and 47 control subjects were enrolled in the study. CDH13 promoter methylation was detected in 44 out of 98 (44.9%) patients with prostate cancer; no methylation was found in control subjects. Methylation of CDH13 was significantly associated with an increased Gleason score, an advanced tumour stage, and a high prostate-specific antigen level. CDH13 methylation was associated with a worse survival outcome and a relative risk of death of 6.132 (95% confidence interval: 3.160, 12.187).
  
  
  CONCLUSIONS
  Promoter methylation of CDH13 occurred frequently in the serum of patients with prostate cancer, was associated with an increased risk of death, and may become a useful independent predictor of a poor prognosis.
authors.affiliation: Department of Urology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Urology, Xuzhou Tumour Hospital, Xuzhou, Jiangsu Province, China.
authors.forename: Ying-Li
authors.initials: YL
authors.lastname: Lin
authors.affiliation: Department of Urology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Urology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei Province, China.
authors.forename: Yu-Zhong
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: Department of Urology, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China lwp2014sjz@163.com.
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Li
authors.affiliation: Department of Urology, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Ma
mesh_terms: D000368:Aged; D015820:Cadherins / Q000097:blood* / Q000235:genetics*; D016022:Case-Control Studies; D019175:DNA Methylation; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011446:Prospective Studies; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D015996:Survival Rate
chemical_list: D015820:Cadherins; C099972:H-cadherin
keywords: Prostate cancer; cadherin 13; methylation; serum