title: Bioactivity of essential oil of Artemisia argyi Lévl. et Van. and its main compounds against Lasioderma serricorne.
abstract: Artemisia argyi Lévl. et Van., a perennial herb with a strong volatile odor, is widely distrbuted in the world. Essential oil obtained from Artemisia argyi was analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 32 components representing 91.74% of the total oil were identified and the main compounds in the oil were found to be eucalyptol (22.03%), β-pinene (14.53%), β-caryophyllene (9.24%) and (-)-camphor (5.45%). With a further isolation, four active constituents were obtained from the essential oil and identified as eucalyptol, β-pinene, β-caryophyllene and camphor. The essential oil and the four isolated compounds exhibited potential bioactivity against Lasioderma serricorne adults. In the progress of assay, it showed that the essential oil, camphor, eucalyptol, β-caryophyllene and β-pinene exhibited strong contact toxicity against L. serricorne adults with LD50 values of 6.42, 11.30, 15.58, 35.52, and 65.55 μg/adult, respectively. During the fumigant toxicity test, the essential oil, eucalyptol and camphor showed stronger fumigant toxicity against L. serricorne adults than β-pinene (LC50 = 29.03 mg/L air) with LC50 values of 8.04, 5.18 and 2.91 mg/L air. Moreover, the essential oil, eucalyptol, β-pinene and camphor also exhibited the strong repellency against L. serricorne adults, while, β-caryophyllene exhibited attracting activity relative to the positive control, DEET. The study revealed that the bioactivity properties of the essential oil can be attributed to the synergistic effects of its diverse major and minor components. The results indicate that the essential oil of A. argyi and the isolated compounds have potential to be developed into natural insecticides, fumigants or repellents in controlling insects in stored grains and traditional Chinese medicinal materials.
authors.affiliation: Protection and Utilization of Traditional Chinese Medicine of Beijing Area Major Laboratory, College of Resources Science and Technology, Beijing Normal University.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Chun-Xue
authors.initials: CX
authors.lastname: You
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.forename: Ran
authors.initials: R
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Zhu-Feng
authors.initials: ZF
authors.lastname: Geng
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Chen
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Jiang
authors.forename: Yang
authors.initials: Y
authors.lastname: Su
authors.forename: Ning
authors.initials: N
authors.lastname: Lei
authors.forename: Ping
authors.initials: P
authors.lastname: Ma
authors.forename: Shu-Shan
authors.initials: SS
authors.lastname: Du
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Deng
mesh_terms: D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D001517:Coleoptera / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000737:chemistry; D007928:Lethal Dose 50; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D007306:Insecticides; D009822:Oils, Volatile