title: Sensitive detection of microRNA by chronocoulometry and rolling circle amplification on a gold electrode.
abstract: We developed a quantitative detection scheme for nucleic acids, combining solid-phase rolling circle amplification and chronocoulometry (RCA-CC). A gold electrode was directly formed on a polystyrene substrate as a cost-effective and flexible biosensor for sensitive detection of microRNA (mir-143) in blood samples.
authors.affiliation: Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China. yaobo08@zju.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Yao
authors.forename: Yichen
authors.initials: Y
authors.lastname: Liu
authors.forename: Miyuki
authors.initials: M
authors.lastname: Tabata
authors.forename: Huangtianzhi
authors.initials: H
authors.lastname: Zhu
authors.forename: Yuji
authors.initials: Y
authors.lastname: Miyahara
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D035683:MicroRNAs / Q000097:blood*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation
chemical_list: D035683:MicroRNAs; D006046:Gold