title: Effective method for the heat inactivation of Blastomyces dermatitidis.
abstract: Manipulation of Blastomyces dermatitidis requires the use of containment level 3 (CL3) practices. However, access to CL3 laboratories is limited and working conditions are restrictive. We describe the validation of a "heat-killing" method to inactivate B. dermatitidis, thus allowing cellular material to be removed from the CL3 laboratory for subsequent DNA isolation that is suitable for genetic applications.
authors.affiliation: Public Health Laboratories Toronto, Public Health Ontario, Canada Department of Microbiology, Mount Sinai Hospital, Toronto, Ontario, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Brown
authors.affiliation: Public Health Laboratories Toronto, Public Health Ontario, Canada lisa.mctaggart@oahpp.ca.
authors.forename: Lisa R
authors.initials: LR
authors.lastname: McTaggart
authors.affiliation: Department of Microbiology, Mount Sinai Hospital, Toronto, Ontario, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada.
authors.forename: Donald E
authors.initials: DE
authors.lastname: Low
authors.affiliation: Public Health Laboratories Toronto, Public Health Ontario, Canada Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada Division of Microbiology, Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: Susan E
authors.initials: SE
authors.lastname: Richardson
mesh_terms: D000818:Animals; D001758:Blastomyces / Q000235:genetics / Q000528:radiation effects*; D003264:Containment of Biohazards; D004203:Disinfection / Q000379:methods*; D005827:Genetics, Microbial / Q000379:methods; D006358:Hot Temperature; D006801:Humans; D050296:Microbial Viability / Q000528:radiation effects*; D008967:Molecular Biology / Q000379:methods; D017751:Safety Management / Q000379:methods*
keywords: Blastomyces dermatitidis; deactivation of Blastomyces dermatitidis; heat inactivation; inactivation of Blastomyces dermatitidis