title: Use of fractional factorial design to study the compatibility of viral ribonucleoprotein gene segments of human H7N9 virus and circulating human influenza subtypes.
abstract: Avian H7N9 influenza viruses may pose a further threat to humans by reassortment with human viruses, which could lead to generation of novel reassortants with enhanced polymerase activity. We previously established a novel statistical approach to study the polymerase activity of reassorted vRNPs (Influenza Other Respir Viruses. 2013;7:969-78). Here, we report the use of this method to study recombinant vRNPs with subunits derived from human H1N1, H3N2, and H7N9 viruses. Our results demonstrate that some reassortant vRNPs with subunits derived from the H7N9 and other human viruses can have much higher polymerase activities than the wild-type levels.
authors.affiliation: Centre of Influenza Research and School of Public Health, The University of Hong Kong, Hong Kong SAR, China.
authors.forename: Alex W H
authors.initials: AW
authors.lastname: Chin
authors.forename: Chris K P
authors.initials: CK
authors.lastname: Mok
authors.forename: Huachen
authors.initials: H
authors.lastname: Zhu
authors.forename: Yi
authors.initials: Y
authors.lastname: Guan
authors.forename: Joseph S M
authors.initials: JS
authors.lastname: Peiris
authors.forename: Leo L M
authors.initials: LL
authors.lastname: Poon
mesh_terms: D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000378:metabolism; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000378:metabolism; D064766:Influenza A Virus, H7N9 Subtype / Q000235:genetics* / Q000378:metabolism; D007251:Influenza, Human / Q000821:virology*; D016865:Reassortant Viruses / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012261:Ribonucleoproteins; D014764:Viral Proteins
keywords: Fractional factorial design; H7N9; influenza polymerase