title: Interferon regulatory factor 5-dependent immune responses in the draining lymph node protect against West Nile virus infection.
abstract: Upon activation of Toll-like and RIG-I-like receptor signaling pathways, the transcription factor IRF5 translocates to the nucleus and induces antiviral immune programs. The recent discovery of a homozygous mutation in the immunoregulatory gene guanine exchange factor dedicator of cytokinesis 2 (Dock2mu/mu) in several Irf5-/- mouse colonies has complicated interpretation of immune functions previously ascribed to IRF5. To define the antiviral functions of IRF5 in vivo, we infected backcrossed Irf5-/-×Dock2wt/wt mice (here called Irf5-/- mice) and independently generated CMV-Cre Irf5fl/fl mice with West Nile virus (WNV), a pathogenic neurotropic flavivirus. Compared to congenic wild-type animals, Irf5-/- and CMV-Cre Irf5fl/fl mice were more vulnerable to WNV infection, and this phenotype was associated with increased infection in peripheral organs, which resulted in higher virus titers in the central nervous system. The loss of IRF5, however, was associated with only small differences in the type I interferon response systemically and in the draining lymph node during WNV infection. Instead, lower levels of several other proinflammatory cytokines and chemokines, as well as fewer and less activated immune cells, were detected in the draining lymph node 2 days after WNV infection. WNV-specific antibody responses in Irf5-/- mice also were blunted in the context of live or inactivated virus infection and this was associated with fewer antigen-specific memory B cells and long-lived plasma cells. Our results with Irf5-/- mice establish a key role for IRF5 in shaping the early innate immune response in the draining lymph node, which impacts the spread of virus infection, optimal B cell immunity, and disease pathogenesis.
  
  
  OBJECTIVE
  Although the roles of IRF3 and IRF7 in orchestrating innate and adaptive immunity after viral infection are established, the function of the related transcription factor IRF5 remains less certain. Prior studies in Irf5-/- mice reported conflicting results as to the contribution of IRF5 in regulating type I interferon and adaptive immune responses. The lack of clarity may stem from a recently discovered homozygous loss-of-function mutation of the immunoregulatory gene Dock2 in several colonies of Irf5-/- mice. Here, using a mouse model with a deficiency in IRF5 and wild-type Dock2 alleles, we investigated how IRF5 modulates West Nile virus (WNV) pathogenesis and host immune responses. Our in vivo studies indicate that IRF5 has a key role in shaping the early proinflammatory cytokine response in the draining lymph node, which impacts immunity and control of WNV infection.
authors.affiliation: Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri, USA.
authors.forename: Larissa B
authors.initials: LB
authors.lastname: Thackray
authors.affiliation: Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri, USA.
authors.forename: Bimmi
authors.initials: B
authors.lastname: Shrestha
authors.affiliation: Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri, USA.
authors.forename: Justin M
authors.initials: JM
authors.lastname: Richner
authors.affiliation: Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri, USA.
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Miner
authors.affiliation: Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri, USA.
authors.forename: Amelia K
authors.initials: AK
authors.lastname: Pinto
authors.affiliation: Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri, USA.
authors.forename: Helen M
authors.initials: HM
authors.lastname: Lazear
authors.affiliation: Department of Immunology, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Gale
authors.affiliation: Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri, USA Department of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, Missouri, USA The Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, Saint Louis, Missouri, USA diamond@borcim.wustl.edu.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Diamond
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology / Q000821:virology; D002490:Central Nervous System / Q000276:immunology / Q000473:pathology / Q000821:virology; D003433:Crosses, Genetic; D005260:Female; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000276:immunology; D017353:Gene Deletion; D015870:Gene Expression / Q000276:immunology; D020662:Guanine Nucleotide Exchange Factors; D007113:Immunity, Innate; D050835:Interferon Regulatory Factors / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology / Q000821:virology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D019562:Viral Load; D014901:West Nile Fever / Q000235:genetics / Q000276:immunology* / Q000821:virology; D014902:West Nile virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; C471356:DOCK2 protein, mouse; D020690:GTPase-Activating Proteins; D020662:Guanine Nucleotide Exchange Factors; D050835:Interferon Regulatory Factors; C498178:Irf5 protein, mouse