title: The glucose RQ-feedback control leading to improved erythromycin production by a recombinant strain Saccharopolyspora erythraea ZL1004 and its scale-up to 372-m(3) fermenter.
abstract: In this paper, glucose respiratory quotient (RQ)-feedback control was developed for erythromycin production with a recombinant strain Saccharopolyspora erythraea ZL1004. RQ was confirmed to be an ideal online parameter for regulating glucose feed rate. Through feeding glucose to control RQ at 0.85 during 45-100 h and 0.95 during 100-185 h, erythromycin titer and erythromycin A concentration were reached 11.88 and 8.82 g l(-1) in 50 l fermenter, which were increased by 8.3 and 6.1 % as compared to that with glucose pH-feedback control, respectively. When glucose RQ-feedback control was scaled up to 372-m(3) fermenter, erythromycin titer and erythromycin A concentration at 155 h were reached 9.12 and 7.12 g l(-1), respectively, which were 10.5 and 9.4 % higher than that with the original technology (glucose pH-feedback control). To the best of our knowledge, this is the first report on the successful application of glucose RQ-feedback control in erythromycin production, especially in 372-m(3) fermenter.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. Box 329, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Zejiang
authors.initials: Z
authors.lastname: Wang
authors.forename: Ju
authors.initials: J
authors.lastname: Chu
authors.forename: Beili
authors.initials: B
authors.lastname: Xi
authors.forename: Yingping
authors.initials: Y
authors.lastname: Zhuang
mesh_terms: D019149:Bioreactors; D004917:Erythromycin / Q000096:biosynthesis*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D011995:Recombination, Genetic; D016930:Saccharopolyspora / Q000235:genetics / Q000378:metabolism*
chemical_list: D004917:Erythromycin; D005947:Glucose