title: A fluorescent nucleic acid nanodevice quantitatively images elevated cyclic adenosine monophosphate in membrane-bound compartments.
abstract: cAMPhor: In the presence of cAMP, cAMPhor folds into a structure that binds DFHBI (green), increasing its fluorescence, while Alexa 647 (red) functions as a normalizing dye. It can thus be used to spatially image cAMP quantitatively in membrane-bound compartments.
authors.affiliation: Department Biophysics and Biochemistry, National Centre for Biological Sciences, UAS-GKVK, Bellary Road, Bangalore-, 560065, India.
authors.forename: Suruchi
authors.initials: S
authors.lastname: Sharma
authors.forename: Anisha
authors.initials: A
authors.lastname: Zaveri
authors.forename: Sandhya S
authors.initials: SS
authors.lastname: Visweswariah
authors.forename: Yamuna
authors.initials: Y
authors.lastname: Krishnan
mesh_terms: D001593:Benzyl Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D000242:Cyclic AMP / Q000032:analysis* / Q000378:metabolism*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005453:Fluorescence; D054884:Host-Pathogen Interactions; D048288:Imidazolines / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D009165:Mycobacterium Infections, Nontuberculous / Q000378:metabolism / Q000473:pathology; D020102:Mycobacterium smegmatis / Q000502:physiology; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D061848:Optical Imaging / Q000295:instrumentation* / Q000379:methods; D012313:RNA / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: C560120:3,5-difluoro-4-hydroxybenzylidene imidazolinone; D001593:Benzyl Compounds; D048288:Imidazolines; D008051:Lipid Bilayers; D009696:Nucleic Acids; D012313:RNA; D000242:Cyclic AMP; D004134:Dimyristoylphosphatidylcholine
keywords: DFHBI; GUVs; aptamers; cAMP; fluorescence; sensors