title: Assessing CpG island methylator phenotype, 1p/19q codeletion, and MGMT promoter methylation from epigenome-wide data in the biomarker cohort of the NOA-04 trial.
abstract: BACKGROUND
  Molecular biomarkers including isocitrate dehydrogenase 1 or 2 (IDH1/2) mutation, 1p/19q codeletion, and O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter methylation may improve prognostication and guide treatment decisions for patients with World Health Organization (WHO) anaplastic gliomas. At present, each marker is individually tested by distinct assays. Illumina Infinium HumanMethylation450 BeadChip arrays (HM450) enable the determination of large-scale methylation profiles and genome-wide DNA copy number changes. Algorithms have been developed to detect the glioma CpG island methylator phenotype (G-CIMP) associated with IDH1/2 mutation, 1p/19q codeletion, and MGMT promoter methylation using a single assay.
  
  
  METHODS
  Here, we retrospectively investigated the diagnostic and prognostic performance of these algorithms in comparison to individual marker testing and patient outcome in the biomarker cohort (n = 115 patients) of the NOA-04 trial.
  
  
  RESULTS
  Concordance for IDH and 1p/19q status was very high: In 92% of samples, the HM450 and reference data agreed. In discordant samples, survival analysis by Kaplan-Meier and Cox regression analyses suggested a more accurate assessment of biological phenotype by the HM450 analysis. The HM450-derived MGMT-STP27 model to calculate MGMT promoter methylation probability revealed this aberration in a significantly higher fraction of samples than conventional methylation-specific PCR, with 87 of 91 G-CIMP tumors predicted as MGMT promoter-methylated. Pyrosequencing of discordant samples confirmed the HM450 assessment in 14 of 17 cases.
  
  
  CONCLUSIONS
  G-CIMP and 1p/19q codeletion are reliably detectable by HM450 analysis and are associated with prognosis in the NOA-04 trial. For MGMT, HM450 suggests promoter methylation in the vast majority of G-CIMP tumors, which is supported by pyrosequencing.
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Benedikt
authors.initials: B
authors.lastname: Wiestler
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: David
authors.initials: D
authors.lastname: Capper
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Volker
authors.initials: V
authors.lastname: Hovestadt
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Martin
authors.initials: M
authors.lastname: Sill
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: David T W
authors.initials: DT
authors.lastname: Jones
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Christian
authors.initials: C
authors.lastname: Hartmann
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Joerg
authors.initials: J
authors.lastname: Felsberg
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Michael
authors.initials: M
authors.lastname: Platten
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Feiden
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Kathy
authors.initials: K
authors.lastname: Keyvani
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Stefan M
authors.initials: SM
authors.lastname: Pfister
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Otmar D
authors.initials: OD
authors.lastname: Wiestler
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Richard
authors.initials: R
authors.lastname: Meyermann
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Guido
authors.initials: G
authors.lastname: Reifenberger
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Thorsten
authors.initials: T
authors.lastname: Pietsch
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Andreas
authors.initials: A
authors.lastname: von Deimling
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Michael
authors.initials: M
authors.lastname: Weller
authors.affiliation: Department of Neurooncology (B.W., M.P., W.W.); Department of Neuropathology (D.C., A.v.D.); Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital (S.M.P.); Clinical Cooperation Units Neurooncology (B.W., W.W.), Neuropathology (D.C, A.v.D.); Neuroimmunology and Brain Tumor Immunology (M.P.); Division of Molecular Genetics (V.H.); Division of Biostatistics (M.S.); Division of Pediatric Neurooncology (S.M.P., D.T.W.J.), German Cancer Research Center (DKFZ), Heidelberg, Germany (O.D.W.) and German Cancer Consortium (DKTK); Department for Neuropathology, Institute of Pathology, Medical University of Hannover, Hannover, Germany (C.H.); Department of Neuropathology, Heinrich-Heine-University, Düsseldorf, Germany (J.F., G.R.); German Consortium for Translational Cancer Research (DKTK), Düsseldorf, Germany (M.P., G.R.); Institute for Neuropathology, Saarland University, 66421 Saarbrücken, Germany (W.F.); Institute for Neuropathology, University of Essen Medical School, Essen, Germany (K.K.); Institute for Neuropathology, University of Tübingen, Tübingen, Germany (R.M.); Department of Neuropathology, University of Bonn Medical Center, Bonn, Germany (T.P.); Department of Neurology, University Hospital Zurich, Zürich, Switzerland (M.W.).
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wick
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D015415:Biomarkers; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D018899:CpG Islands; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics; D045643:DNA Repair Enzymes / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D005910:Glioma / Q000175:diagnosis* / Q000235:genetics*; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012189:Retrospective Studies; D025521:Tumor Suppressor Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: D015415:Biomarkers; D025521:Tumor Suppressor Proteins; D007521:Isocitrate Dehydrogenase; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes
keywords: 1p/19q; 450k; G-CIMP; MGMT; anaplastic glioma