title: Immunolocalization of MMP-2 and MMP-9 in human rheumatoid synovium.
abstract: Matrix metalloproteinase (MMP)-2 and MMP-9, two important members of the matrix metalloproteinase family, have been shown critical contributions in intra-tumor angiogenesis and invasion of tumor progression, and they might also play important roles in the angiogenesis as well as the pannus formation of rheumatoid arthritis (RA). In the present study, we used the immunohistochemistry, the immunofluorescence staining and the con-focal scanning methods to characterize the immunolocalization of MMP-2 and MMP-9 in RA synovium tissues. Our results showed that both MMP-2 and MMP-9 immunostaining could be found in synoviocytes and vascular endothelial cells. Moreover, our con-focal scanning also showed that MMP-2 could be found in infiltrating CD14(+) monocytes and CD68(+) macrophages, and MMP-9 could be found in infiltrating CD68(+) macrophages in RA synovium tissues, while weak or negative staining of these two MMPs could be found in infiltrating CD20(+)B cells and CD3(+)T cells in RA synovium. Thus, our finding suggests that both MMP-2 and MMP-9 expressed by synoviocytes as well as certain infiltrating immune cells role importantly in the angiogenesis in RA progression.
authors.affiliation: Department of General Internal Medicine, The Third Affiliated Hospital of Soochow University Changzhou 213003, Jiangsu, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of General Internal Medicine, The Third Affiliated Hospital of Soochow University Changzhou 213003, Jiangsu, China.
authors.forename: Si
authors.initials: S
authors.lastname: Qin
authors.affiliation: Department of General Internal Medicine, The Third Affiliated Hospital of Soochow University Changzhou 213003, Jiangsu, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Testing Center, Center for Disease Prevention and Control Changzhou 213000, Jiangsu, China.
authors.forename: Fengming
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of General Internal Medicine, The Third Affiliated Hospital of Soochow University Changzhou 213003, Jiangsu, China.
authors.forename: Lujun
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of General Internal Medicine, The Third Affiliated Hospital of Soochow University Changzhou 213003, Jiangsu, China.
authors.forename: Yahua
authors.initials: Y
authors.lastname: Lu
mesh_terms: D001172:Arthritis, Rheumatoid / Q000201:enzymology* / Q000473:pathology; D042783:Endothelial Cells / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007719:Knee Joint; D008264:Macrophages / Q000201:enzymology; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000096:biosynthesis*; D018613:Microscopy, Confocal; D009000:Monocytes / Q000201:enzymology; D009389:Neovascularization, Pathologic / Q000201:enzymology; D013583:Synovial Membrane / Q000201:enzymology* / Q000473:pathology
chemical_list: C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Matrix metalloproteinase-2; confocal scanning; matrix metalloproteinase-9; rheumatoid arthritis