title: The RcsCDB regulatory system plays a crucial role in the protection of Salmonella enterica serovar Typhimurium against oxidative stress.
abstract: Dps, the most abundant protein during the stationary growth phase, in Salmonella enterica is required for resistance to reactive oxygen species produced by the host during infection. It has been reported that in Salmonella dps expression is controlled by RpoS and Fur proteins. However, the regulation and function of Dps remain to be resolved. In the present work we demonstrate that activation of the complex RcsCDB regulatory system increases dps expression during exponential growth of Salmonella. In addition, we show that such dps upregulation produces high levels of H2O2 resistance. This phenotype allows the bacteria to avoid reactive oxygen species killing at early stages of growth, thus protecting its genetic material.
authors.affiliation: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica 'Dr. Bernabé Bloj', Facultad de Bioquímica, Química y Farmacia, UNT. Chacabuco 461, T4000ILI - San Miguel de Tucumán, Argentina.
authors.forename: Juan V
authors.initials: JV
authors.lastname: Farizano
authors.affiliation: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica 'Dr. Bernabé Bloj', Facultad de Bioquímica, Química y Farmacia, UNT. Chacabuco 461, T4000ILI - San Miguel de Tucumán, Argentina.
authors.forename: Mariela A
authors.initials: MA
authors.lastname: Torres
authors.affiliation: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica 'Dr. Bernabé Bloj', Facultad de Bioquímica, Química y Farmacia, UNT. Chacabuco 461, T4000ILI - San Miguel de Tucumán, Argentina.
authors.forename: María de Las Mercedes
authors.initials: MLM
authors.lastname: Pescaretti
authors.affiliation: Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica 'Dr. Bernabé Bloj', Facultad de Bioquímica, Química y Farmacia, UNT. Chacabuco 461, T4000ILI - San Miguel de Tucumán, Argentina.
authors.forename: Mónica A
authors.initials: MA
authors.lastname: Delgado
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004361:Drug Tolerance; D015964:Gene Expression Regulation, Bacterial; D053263:Gene Regulatory Networks; D050296:Microbial Viability; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000633:toxicity; D012486:Salmonella typhimurium / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C083911:DPS protein, Bacteria; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C495282:YojN protein, Salmonella typhimurium; C074889:RcsB protein, Bacteria