title: Increased Meningeal T and Plasma Cell Infiltration is Associated with Early Subpial Cortical Demyelination in Common Marmosets with Experimental Autoimmune Encephalomyelitis.
abstract: Subpial cortical demyelination (SCD) accounts for the greatest proportion of demyelinated cortex in multiple sclerosis (MS). SCD is already found in biopsy cases with early MS and in marmosets with experimental autoimmune encephalomyelitis (EAE), but the pathogenesis of SCD is not well understood. The objective of this study was to investigate whether and, if so, which meningeal inflammatory cells were associated with early SCD in marmosets with EAE. Immunohistochemistry was performed to analyze brain samples from eight control animals and eight marmosets immunized with myelin oligodendrocyte glycoprotein. Meningeal T, B and plasma cells were quantified adjacent to SCD, normal-appearing EAE cortex (NAC) and control marmoset cortex. SCD areas appeared mostly hypocellular with low-grade microglial activation. In marmosets with EAE, meninges adjacent to SCD showed significantly increased T cells paralleled by elevated plasma cells, but unaltered B cell numbers compared with NAC. The elevation of meningeal T and plasma cells was a specific finding topographically associated with SCD, as the meninges overlying NAC displayed similarly low T, B and plasma cell numbers as control cortex. These findings suggest that local meningeal T and plasma cell infiltration contributes to the pathogenesis of SCD in marmosets with EAE.
authors.affiliation: Institute of Neuropathology, University Medical Center Göttingen, Göttingen, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Kramann
authors.forename: Katharina
authors.initials: K
authors.lastname: Neid
authors.forename: Lena
authors.initials: L
authors.lastname: Menken
authors.forename: Christina
authors.initials: C
authors.lastname: Schlumbohm
authors.forename: Christine
authors.initials: C
authors.lastname: Stadelmann
authors.forename: Eberhard
authors.initials: E
authors.lastname: Fuchs
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Brück
authors.forename: Christiane
authors.initials: C
authors.lastname: Wegner
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D040502:Calgranulin B / Q000378:metabolism; D002144:Callithrix; D016022:Case-Control Studies; D002540:Cerebral Cortex / Q000473:pathology*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D005260:Female; D008297:Male; D008578:Meninges / Q000473:pathology*; D009103:Multiple Sclerosis / Q000473:pathology*; D004676:Myelin Basic Protein / Q000378:metabolism; D010950:Plasma Cells / Q000378:metabolism / Q000473:pathology*; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology*; D066127:White Matter / Q000473:pathology
chemical_list: D015703:Antigens, CD; D040502:Calgranulin B; D004676:Myelin Basic Protein
keywords: cortical demyelination; experimental autoimmune encephalomyelitis; marmoset; meningeal inflammation; multiple sclerosis; neuropathology