title: Autofluorescence (safe) bronchoscopy and p21/ki-67 immunostaining related to carcinogenesis.
abstract: Archival biopsy materials from 20 randomly selected asymptomatic volunteers from the Czech uranium miners (CZ UM) risk group (n=98) were examined for p21 and ki-67 immunostatning. There were 16 areas with normal respiratory epithelium and 22 areas with bronchial intra-epithelial neoplasia (IEN). Normal and IEN areas were identified by autofluorescence (System Autofluorescence Endoscopy, SAFE-1000) and monitored during 1998-2002. The majority of specimens from areas with normal autofluorescence intensity with ciliated columnar bronchial epithelium showed strong predominantly cytoplasmic p21 positivity. The SAFE monitoring divided areas of decreased autofluorescence intensity with early stage IEN lesions into two groups. Persistent lesions (P)-showing a spectrum of p21 cytoplasmic staining ranging from negative or isolated negativity to weak or moderate positivity combined with higher proliferative capacity proved by ki-67 nuclear staining. Disappearing lesions (D)-showing strong cytoplasmic p21 positivity and negative ki-67 staining. The IEN lesions were classified into three groups based on p21/ki-67 immunostaining: proliferative lesions at risk (R) with low or without p21 plasma immunostaining combined with high ki-67 nuclear reactivity; ambiguous lesions (A) including cases combining strong p21 cytoplasmic positivity with high ki-67 nuclear reactivity or p21 cytoplasmic negativity with ki-67 negativity staining patterns; the quiescent lesion group (Q) was characterized by strong p21 cytoplasmic positivity and negative ki-67 immunostaining.
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: T A
authors.initials: TA
authors.lastname: Horvath
authors.affiliation: First Department of Surgery, Tokyo Medical University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hirano
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: M D
authors.initials: MD
authors.lastname: Horvathova
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Vomela
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: R
authors.initials: R
authors.lastname: Vyzula
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Cı́halová
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: F
authors.initials: F
authors.lastname: Dorociak
authors.affiliation: Department of Histopathology, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ebihara
authors.affiliation: First Department of Surgery, Tokyo Medical University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: B
authors.initials: B
authors.lastname: Habanec
authors.affiliation: First Department of Surgery, Tokyo Medical University, Tokyo, Japan.
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authors.initials: N
authors.lastname: Ikeda
authors.affiliation: First Department of Surgery, Tokyo Medical University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kakihana
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Klusáková
authors.affiliation: Department of Histopathology, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Okada
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Rejthar
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: D
authors.initials: D
authors.lastname: Stratil
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Shaffi-Sahely
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: B
authors.initials: B
authors.lastname: Smajer
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: S
authors.initials: S
authors.lastname: Spelda
authors.affiliation: First Department of Surgery, Tokyo Medical University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tsuboi
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Vagunda
authors.affiliation: Department of Surgery, University Hospital Bohunice, Masaryk Memorial Cancer Institute and University Oncology Center Brno, Žlutý kopec 7, CZ-65653 Brno, Czech Republic.
authors.forename: F
authors.initials: F
authors.lastname: Wurst
authors.affiliation: First Department of Surgery, Tokyo Medical University, Tokyo, Japan.
authors.forename: H
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authors.lastname: Kato