title: Simultaneous quantitation and validation of triterpenoids and phytosteroids in Phaseolus angularis seeds.
abstract: A reproducible analytical method using reverse-phase high liquid performance chromatography combined with UV detecting was developed for the quantitative determination of four compounds isolated from the ethanol extract of Phaseolus angularis seeds (PASE): oleanolic acid (1), oleanolic acid acetate (2), stigmasterol (3) and β-sitosterol (4). This method was fully validated in terms of linearity (r2 > 0.999), accuracy (98.5%-100.8%), precision (<0.92%), LOD (<0.0035 mg/mL), and LOQ (<0.0115 mg/mL). The effects of the PASE and isolated compounds 1-4 on TLR4 activation were tested in THP1-Blue cells. Among the tested substances, compound 2 showed potent inhibitory activity with an IC50 value of 3.89 ± 0.17 µM.
authors.affiliation: Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea.
authors.forename: Joo Tae
authors.initials: JT
authors.lastname: Hwang
authors.affiliation: Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea.
authors.forename: Hyun-Mee
authors.initials: HM
authors.lastname: Oh
authors.affiliation: Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea.
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea.
authors.forename: Hyung Jae
authors.initials: HJ
authors.lastname: Jeong
authors.affiliation: Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea.
authors.forename: Chul
authors.initials: C
authors.lastname: Lee
authors.affiliation: Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea.
authors.forename: Hyun-Jae
authors.initials: HJ
authors.lastname: Jang
authors.affiliation: Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea.
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea.
authors.forename: Chan Sun
authors.initials: CS
authors.lastname: Park
authors.affiliation: Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea. rho-m@kribb.re.kr.
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
mesh_terms: D002851:Chromatography, High Pressure Liquid; D020128:Inhibitory Concentration 50; D027805:Phaseolus / Q000737:chemistry*; D010840:Phytosterols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D010840:Phytosterols; D010936:Plant Extracts; D051197:Toll-Like Receptor 4; D014315:Triterpenes