title: An in vitro study of the impact of 4mT static magnetic field to modify the differentiation rate of rat bone marrow stem cells into primordial germ cells.
abstract: This investigation was performed to evaluate the differentiation capacity and alteration in genes expression patterns during in vitro differentiation of bone marrow stem cells into primordial germ cells using static magnetic field (4mT) and BMP-4 (25ng/ml). The rate of differentiation was investigated using the Real Time-PCR method for tracing expression of differentiation markers (Oct-4, Nanog, C-Myc, Fragilis, Mvh and Stella). Then, immunocytochemical reaction was carried out for detection of marker proteins (Oct4 and Mvh). Increasing of the exposure time of the 4mT SMF (24 and 48h) and treatment time with 25ng/ml BMP4 (48 and 96h) indicated a marked decrease in expression of pluripotency genes (Oct-4, Nanog and C-Myc) and Oct4 protein and increase in primordial germ cell-specific genes (Fragilis, Mvh and Stella) and Mvh protein compared with the control group. Final results showed that in a synergistic manner, the combination of SMF with BMP4 exaggerates the differentiation potential of BMSCs to PGCs by activating the MAPK pathway and altering the Ca(2+) concentration.
authors.affiliation: Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University (TMU), P.O.B 14115-154, Tehran, Iran. Electronic address: Javani@modares.ac.ir.
authors.forename: Fatemeh Javani
authors.initials: FJ
authors.lastname: Jouni
authors.affiliation: Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University (TMU), P.O.B 14115-154, Tehran, Iran. Electronic address: Parviz@modares.ac.ir.
authors.forename: Parviz
authors.initials: P
authors.lastname: Abdolmaleki
authors.affiliation: Department of Genetics, Faculty of Biological Sciences. Tarbiat Modares University (TMU), P.O.B 14115-154, Tehran, Iran. Electronic address: Behmanesh@modares.ac.ir.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Behmanesh
authors.affiliation: Department of Anatomical sciences, Medical Sciences Faculty, Tarbiat Modares University (TMU), P.O.B 14115-175, Tehran, Iran. Electronic address: Movahed.m@modares.ac.ir.
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Movahedin
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000528:radiation effects*; D055415:Bone Morphogenetic Protein 4 / Q000096:biosynthesis; D002454:Cell Differentiation / Q000528:radiation effects*; D005854:Germ Cells / Q000166:cytology / Q000254:growth & development / Q000528:radiation effects*; D066298:In Vitro Techniques; D060526:Magnetic Fields; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D051381:Rats
chemical_list: D000943:Antigens, Differentiation; D055415:Bone Morphogenetic Protein 4; D050814:Octamer Transcription Factor-3
keywords: Bone marrow stem cell; Bone morphogenetic protein 4; Differentiation; Primordial germ cell; Static magnetic field