title: The bacterial metabolite 2-aminoacetophenone promotes association of pathogenic bacteria with flies.
abstract: Bacteria contaminate insects and secrete metabolites that may affect insect behaviour and potentially fitness through unknown mechanisms. Here we show that the 'grape-like' odorant 2-aminoacetophenone (2AA), secreted by Pseudomonas aeruginosa (a ubiquitous opportunistic human pathogen), facilitates attraction to food for several fly species including Musca domestica, Ceratitis capitata and Drosophila melanogaster. Constant feeding on 2AA increases the level of long-term colonization of the flies' intestine by P. aeruginosa. Odour perception is necessary for enhanced attraction to food containing 2AA, and expression in the Drosophila olfactory organs of odorant receptors Or49b and Or10a potentiates, while expression of Or85a inhibits, preference for 2AA. Our study shows that 2AA lures the flies to the bacterial source and increases the extent of colonization of the fly intestine by the bacteria that produce it, as a means to facilitate bacterial dissemination to new locations.
authors.affiliation: 1] Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology Hellas, Nikolaou Plastira 100, 71003 Heraklion, Greece [2].
authors.forename: Stefania-Elisavet
authors.initials: SE
authors.lastname: Kapsetaki
authors.affiliation: 1] Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology Hellas, Nikolaou Plastira 100, 71003 Heraklion, Greece [2].
authors.forename: Ilias
authors.initials: I
authors.lastname: Tzelepis
authors.affiliation: 1] Department of Biological Sciences, University of Cyprus, 2109 Nicosia, Cyprus [2].
authors.forename: Kalodoti
authors.initials: K
authors.lastname: Avgousti
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology Hellas, Nikolaou Plastira 100, 71003 Heraklion, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Livadaras
authors.affiliation: Institute for Olive Tree & Subtropical Plants, National Agricultural Research Foundation, Agrokipio, 73100 Chania, Greece.
authors.forename: Nikos
authors.initials: N
authors.lastname: Garantonakis
authors.affiliation: Institute for Olive Tree & Subtropical Plants, National Agricultural Research Foundation, Agrokipio, 73100 Chania, Greece.
authors.forename: Kyriaki
authors.initials: K
authors.lastname: Varikou
authors.affiliation: 1] Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology Hellas, Nikolaou Plastira 100, 71003 Heraklion, Greece [2] Department of Biological Sciences, University of Cyprus, 2109 Nicosia, Cyprus.
authors.forename: Yiorgos
authors.initials: Y
authors.lastname: Apidianakis
mesh_terms: D000098:Acetophenones / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D058491:Bacterial Load; D001522:Behavior, Animal / Q000187:drug effects; D033622:Ceratitis capitata / Q000502:physiology; D004175:Diptera / Q000187:drug effects / Q000382:microbiology / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000382:microbiology / Q000502:physiology; D006793:Houseflies / Q000502:physiology; D007422:Intestines / Q000382:microbiology; D009812:Odorants; D011550:Pseudomonas aeruginosa / Q000378:metabolism* / Q000472:pathogenicity; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism
chemical_list: D000098:Acetophenones; D029721:Drosophila Proteins; D018035:Receptors, Odorant; C055495:2-aminoacetophenone