title: Copper(II) complexes with 4-hydroxyacetophenone-derived acylhydrazones: synthesis, characterization, DNA binding and cleavage properties.
abstract: Two new Cu(II) complexes of Schiff base-hydrazone ligands, hydroxy-N'-[(1Z)-1-(4-hydroxyphenyl)ethylidene]benzohydrazide [H₃L(1)] and ethyl 2-(4-(1-(2-(4-(2-ethoxy-2-oxoethoxy)benzoyl)hydrazono)ethyl)phenoxy)acetate (HL(2)) have been synthesized and then characterized by microcopy and spectral studies. X-ray powder diffraction illustrates that [Cu(L(2))₂] complex is crystalline in nature whereas [Cu(H₂L(1))₂]·2H₂O has an amorphous structure. Binding of the copper complexes with Calf thymus DNA (CT-DNA) has been investigated by UV-visible spectra, exhibiting non-covalent binding to CT-DNA. DNA cleavage experiments have been also investigated by agarose gel electrophoresis in the presence and absence of an oxidative agent (H₂O₂). The effect of complex concentration on the DNA cleavage reaction has been also studied. Both copper complexes show nuclease activity, which significantly depends on concentrations of the complexes, in the presence of H₂O₂ through oxidative mechanism whereas they slightly cleavage DNA in the absence an oxidative agent.
authors.affiliation: Department of Chemistry, Mugla Sıtkı Koçman University, 48100 Mugla, Turkey. Electronic address: rgup@mu.edu.tr.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Gup
authors.affiliation: Department of Chemistry, Mugla Sıtkı Koçman University, 48100 Mugla, Turkey.
authors.forename: Cansu
authors.initials: C
authors.lastname: Gökçe
authors.affiliation: Department of Physic, Mugla Sıtkı Koçman University, 48100 Mugla, Turkey.
authors.forename: Selçuk
authors.initials: S
authors.lastname: Aktürk
mesh_terms: D000098:Acetophenones / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D053837:DNA Cleavage / Q000187:drug effects*; D006835:Hydrazones / Q000737:chemistry* / Q000494:pharmacology; D008958:Models, Molecular
chemical_list: D000098:Acetophenones; D056831:Coordination Complexes; D006835:Hydrazones; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; C031335:4-hydroxyacetophenone
keywords: Acylhydrazone; Copper(II) complex; DNA binding; DNA cleavage