title: Effect of tempol on redox homeostasis and stress tolerance in mimetically aged Drosophila.
abstract: We aimed to test our hypothesis that scavenging reactive oxygen species (ROS) with tempol, a membrane permeable antioxidant, affects the type and magnitude of oxidative damage and stress tolerance through mimetic aging process in Drosophila. Drosophila colonies were randomly divided into three groups: (1) no D-galactose, no tempol; (2) D-galactose without tempol; (3) D-galactose, but with tempol. Mimetic aging was induced by d-galactose administration. The tempol-administered flies received tempol at the concentration of 0.2% in addition to d-galactose. Thiobarbituric acid reacting substance (TBARS) concentrations, advanced oxidation protein products (AOPPs), Cu,Zn-superoxide dismutase (Cu,Zn-SOD), sialic acid (SA) were determined. Additionally, stress tolerances were tested. Mimetically aged group without tempol led to a significant decrease in tolerance to heat, cold, and starvation (P < 0.05), but tempol was used for these parameters. The Cu,Zn-SOD activity and SA concentrations were lower in both mimetically aged and tempol-administered Drosophila groups compared to control (P < 0.05), whereas there were no significantly difference between mimetically aged and tempol-administered groups. Mimetically aged group without tempol led to a significant increase in tissue TBARS and AOPPs concentrations (P < 0.05). Coadministration of tempol could prevent these alterations. Scavenging ROS using tempol also restores redox homeostasis in mimetically aged group. Tempol partly restores age-related oxidative injury and increases stress tolerance.
authors.affiliation: Department of Biology, Science Faculty, Zoology Division, Istanbul University, Istanbul, Turkey.
authors.forename: Ugur
authors.initials: U
authors.lastname: Aksu
authors.forename: Karolin
authors.initials: K
authors.lastname: Yanar
authors.forename: Duygu
authors.initials: D
authors.lastname: Terzioglu
authors.forename: Tugçe
authors.initials: T
authors.lastname: Erkol
authors.forename: Evrim
authors.initials: E
authors.lastname: Ece
authors.forename: Seval
authors.initials: S
authors.lastname: Aydin
authors.forename: Ezel
authors.initials: E
authors.lastname: Uslu
authors.forename: Ufuk
authors.initials: U
authors.lastname: Çakatay
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D004330:Drosophila / Q000378:metabolism / Q000502:physiology*; D005690:Galactose / Q000009:adverse effects / Q000378:metabolism; D006706:Homeostasis / Q000502:physiology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013113:Spin Labels; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003497:Cyclic N-Oxides; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013482:Superoxide Dismutase; C001803:tempol; D005690:Galactose
keywords: Drosophila; mimetic aging; oxidative stress; sialic acid; tempol