title: Isolation of Chandipura virus (Vesiculovirus: Rhabdoviridae) from Sergentomyia species of sandflies from Nagpur, Maharashtra, India.
abstract: OBJECTIVE
  An outbreak of acute encephalitis syndrome was reported from Vidarbha region of Maharashtra s0 tate, India, during July 2012. Anti-IgM antibodies against Chandipura virus (CHPV) were detected in clinical samples. Sandfly collections were done to determine their role in CHPV transmission.
  
  
  METHODS
  Twenty nine pools of Sergentomyia spp. comprising 625 specimens were processed for virus isolation in Vero E6 cell line. Diagnostic RT-PCR targeting N-gene was carried out with the sample that showed cytopathic effects (CPE). The PCR product was sequenced, analysed and the sequences were deposited in Genbank database.
  
  
  RESULTS
  CPE in Vero E6 cell line infected with three pools was detected at 48 h post infection. However, virus could be isolated only from one pool. RT-PCR studies demonstrated 527 nucleotide product that confirmed the agent as CHPV. Sequence analysis of the new isolate showed difference in 10-12 nucleotides in comparison to earlier isolates.
  
  
  CONCLUSIONS
  This is perhaps the first isolation of CHPV from Sergentomyia spp. in India and virus isolation during transmission season suggests their probable role in CHPV transmission. Further studies need to be done to confirm the precise role of Sargentomyia spp. in CHPV transmission.
authors.affiliation: Microbial Containment Complex, National Institute of Virology (ICMR), Pune, India.
authors.forename: A B
authors.initials: AB
authors.lastname: Sudeep
authors.forename: V P
authors.initials: VP
authors.lastname: Bondre
authors.forename: Y K
authors.initials: YK
authors.lastname: Gurav
authors.forename: M D
authors.initials: MD
authors.lastname: Gokhale
authors.forename: G N
authors.initials: GN
authors.lastname: Sapkal
authors.forename: M S
authors.initials: MS
authors.lastname: Mavale
authors.forename: R P
authors.initials: RP
authors.lastname: George
authors.forename: A C
authors.initials: AC
authors.lastname: Mishra
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification; D002522:Chlorocebus aethiops; D004660:Encephalitis / Q000453:epidemiology / Q000821:virology; D007194:India; D010691:Phlebotomus / Q000472:pathogenicity* / Q000821:virology; D011576:Psychodidae / Q000472:pathogenicity / Q000821:virology*; D018353:Rhabdoviridae Infections / Q000453:epidemiology / Q000635:transmission* / Q000821:virology; D014709:Vero Cells; D018116:Vesiculovirus / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D000888:Antibodies, Anti-Idiotypic; C044203:anti-IgM