title: Vibrational spectroscopic methods for cytology and cellular research.
abstract: The use of vibrational spectroscopy, FTIR and Raman, for cytology and cellular research has the potential to revolutionise the approach to cellular analysis. Vibrational spectroscopy is non-destructive, simple to operate and provides direct information. Importantly it does not require expensive exogenous labels that may affect the chemistry of the cell under analysis. In addition, the advent of spectroscopic microscopes provides the ability to image cells and acquire spectra with a subcellular resolution. This introductory review focuses on recent developments within this fast paced field and highlights potential for the future use of FTIR and Raman spectroscopy. We particularly focus on the development of live cell research and the new technologies and methodologies that have enabled this.
authors.affiliation: Centre for Materials Science, Division of Chemistry, University of Central Lancashire, Preston, Lancashire PR1 2HE, UK. gclemens@uclan.ac.uk.
authors.forename: Graeme
authors.initials: G
authors.lastname: Clemens
authors.forename: James R
authors.initials: JR
authors.lastname: Hands
authors.forename: Konrad M
authors.initials: KM
authors.lastname: Dorling
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Baker
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*