title: Osteoblast-like cell behavior on porous scaffolds based on poly(styrene) fibers.
abstract: Scaffolds of nonresorbable biomaterials can represent an interesting alternative for replacing large bone defects in some particular clinical cases with massive bone loss. Poly(styrene) microfibers were prepared by a dry spinning method. They were partially melted to provide 3D porous scaffolds. The quality of the material was assessed by Raman spectroscopy. Surface roughness was determined by atomic force microscopy and vertical interference microscopy. Saos-2 osteoblast-like cells were seeded on the surface of the fibers and left to proliferate. Cell morphology, evaluated by scanning electron microscopy, revealed that they can spread and elongate on the rough microfiber surface. Porous 3D scaffolds made of nonresorbable poly(styrene) fibers are cytocompatible biomaterials mimicking allogenic bone trabeculae and allowing the growth and development of osteoblast-like cells in vitro.
authors.affiliation: Advanced Polymer Materials Group, University Politehnica of Bucharest, 149 Calea Victoriei, Sector 1, 010072 Bucharest, Romania.
authors.forename: Andrada
authors.initials: A
authors.lastname: Serafim
authors.affiliation: GEROM Groupe Etudes Remodelage Osseux et bioMatériaux-LHEA, IRIS-IBS Institut de Biologie en Santé, LUNAM Université, 49933 Angers Cedex, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Mallet
authors.affiliation: GEROM Groupe Etudes Remodelage Osseux et bioMatériaux-LHEA, IRIS-IBS Institut de Biologie en Santé, LUNAM Université, 49933 Angers Cedex, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Pascaretti-Grizon
authors.affiliation: Advanced Polymer Materials Group, University Politehnica of Bucharest, 149 Calea Victoriei, Sector 1, 010072 Bucharest, Romania.
authors.forename: Izabela-Cristina
authors.identifier: 0000-0003-0685-3947
authors.initials: IC
authors.lastname: Stancu
authors.affiliation: GEROM Groupe Etudes Remodelage Osseux et bioMatériaux-LHEA, IRIS-IBS Institut de Biologie en Santé, LUNAM Université, 49933 Angers Cedex, France.
authors.forename: Daniel
authors.identifier: 0000-0002-5886-9513
authors.initials: D
authors.lastname: Chappard
mesh_terms: D001862:Bone Resorption / Q000473:pathology* / Q000628:therapy; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004547:Elastic Tissue / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000737:chemistry / Q000648:ultrastructure*; D011108:Polymers / Q000737:chemistry / Q000627:therapeutic use; D016062:Porosity; D013059:Spectrum Analysis, Raman; D020058:Styrene / Q000737:chemistry / Q000627:therapeutic use; D054457:Tissue Scaffolds
chemical_list: D011108:Polymers; D020058:Styrene