title: Vascular accumulation of the small leucine-rich proteoglycan decorin in CADASIL.
abstract: Small penetrating brain artery thickening is a major feature of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Although affected fibrotic arteries of CADASIL have been shown to accumulate collagen, other components that compose pathological arterial walls remain incompletely characterized. We investigated the expression of decorin (DCN), the first collagen-binding small leucine-rich proteoglycan identified, in CADASIL. DCN was markedly upregulated in pathologically affected leptomeningeal and small penetrating arteries in CADASIL and was notably weaker in normal arteries from control brains. DCN protein was localized principally to the media and adventitia and only occasionally expressed in the intima. Immunoblotting of brain lysates showed a three-fold increase of DCN in CADASIL brains (compared with controls). Messenger RNA encoding DCN was five-fold increased in CADASIL. We conclude that DCN is the first identified proteoglycan to be identified in CADASIL arteries and may accumulate through transcriptional mechanisms. Additional studies are warranted to determine whether DCN localizes broadly to pathological small vessels in other cerebrovascular disorders.
authors.affiliation: Departments of aNeurology bMolecular & Integrative Physiology, University of Michigan cNeurology Service, VA Ann Arbor Healthcare System, Ann Arbor, Michigan, USA.
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Lee
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Zhang
authors.forename: Michael M
authors.initials: MM
authors.lastname: Wang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001158:Arteries / Q000378:metabolism*; D015153:Blotting, Western; D001921:Brain / Q000098:blood supply / Q000378:metabolism*; D046589:CADASIL / Q000378:metabolism*; D058575:Decorin / Q000378:metabolism*; D006801:Humans; D007930:Leucine; D008578:Meninges / Q000098:blood supply / Q000378:metabolism*; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C546878:DCN protein, human; D058575:Decorin; D012333:RNA, Messenger; D007930:Leucine