title: Paradoxical role of DNA methylation in activation of FoxA2 gene expression during endoderm development.
abstract: The transcription factor FoxA2 is a master regulator of endoderm development and pancreatic beta cell gene expression. To elucidate the mechanisms underlying the activation of the FoxA2 gene during differentiation, we have compared the epigenetic status of undifferentiated human embryonic stem cells (hESCs), hESC-derived early endoderm stage cells (CXCR4+ cells), and pancreatic islet cells. Unexpectedly, a CpG island in the promoter region of the FoxA2 gene displayed paradoxically high levels of DNA methylation in expressing tissues (CXCR4+, islets) and low levels in nonexpressing tissues. This CpG island region was found to repress reporter gene expression and bind the Polycomb group protein SUZ12 and the DNA methyltransferase (DNMT)3b preferentially in undifferentiated hESCs as compared with CXCR4+ or islets cells. Consistent with this, activation of FoxA2 gene expression, but not CXCR4 or SOX17, was strongly inhibited by 5-aza-2'-deoxycytidine and by knockdown of DNMT3b. We hypothesize that in nonexpressing tissues, the lack of DNA methylation allows the binding of DNA methyltransferases and repressing proteins, such as Polycomb group proteins; upon differentiation, DNMT activation leads to CpG island methylation, causing loss of repressor protein binding. These results suggest a novel and unexpected role for DNA methylation in the activation of FoxA2 gene expression during differentiation.
authors.affiliation: From the Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Keren
authors.initials: K
authors.lastname: Bahar Halpern
authors.affiliation: From the Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Tal
authors.initials: T
authors.lastname: Vana
authors.affiliation: From the Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel m.walker@weizmann.ac.il.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Walker
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D019175:DNA Methylation; D017931:DNA Primers; D004707:Endoderm / Q000254:growth & development*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D051542:Hepatocyte Nuclear Factor 3-beta / Q000235:genetics*; D006801:Humans; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C496502:FOXA2 protein, human; D051542:Hepatocyte Nuclear Factor 3-beta
keywords: DNA Methylation; Development; Endoderm; Polycomb; Transcription; Transcription Enhancer