title: Development of a fluorescence-based in vivo phagocytosis assay to measure mononuclear phagocyte system function in the rat.
abstract: The mononuclear phagocyte system (MPS) which provides protection against infection is made up of phagocytic cells that engulf and digest bacteria or other foreign substances. Suppression of the MPS may lead to decreased clearance of pathogenic microbes. Drug delivery systems and immunomodulatory therapeutics that target phagocytes have a potential to inhibit MPS function. Available methods to measure inhibition of MPS function use uptake of radioactively-labeled cells or labor-intensive semi-quantitative histologic techniques. The objective of this work was to develop a non-radioactive quantitative method to measure MPS function in vivo by administering heat-killed E. coli conjugated to a pH-sensitive fluorescent dye (Bioparticles(®)). Fluorescence of the Bioparticles(®) is increased at low pH when they are in phagocytic lysosomes. The amount of Bioparticles(®) phagocytosed by MPS organs in rats was determined by measuring fluorescence intensity in livers and spleens ex vivo using an IVIS(®) Spectrum Pre-clinical In Vivo Imaging System. Phagocytosis of the particles by peripheral blood neutrophils was measured by flow cytometry. To assess method sensitivity, compounds likely to suppress the MPS [clodronate-containing liposomes, carboxylate-modified latex particles, maleic vinyl ether (MVE) polymer] were administered to rats prior to injection of the Bioparticles(®). The E. coli particles consistently co-localized with macrophage markers in the liver but not in the spleen. All of the compounds tested decreased phagocytosis in the liver, but had no consistent effects on phagocytic activity in the spleen. In addition, administration of clodronate liposomes and MVE polymer increased the percentage of peripheral blood neutrophils that phagocytosed the Bioparticles(®). In conclusion, an in vivo rat model was developed that measures phagocytosis of E. coli particles in the liver and may be used to assess the impact of test compounds on MPS function. Still, the detection of inhibition of splenic macrophage function will require further assay development.
authors.affiliation: Pfizer Worldwide Research and Development, Drug Safety Research and Development , Groton, CT 06340 , USA.
authors.forename: Karrie
authors.initials: K
authors.lastname: Tartaro
authors.forename: Maria
authors.initials: M
authors.lastname: VanVolkenburg
authors.forename: Dean
authors.initials: D
authors.lastname: Wilkie
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Coskran
authors.forename: John M
authors.initials: JM
authors.lastname: Kreeger
authors.forename: Thomas T
authors.initials: TT
authors.lastname: Kawabata
authors.forename: Sandra
authors.initials: S
authors.lastname: Casinghino
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D004002:Clodronic Acid / Q000008:administration & dosage; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry; D006358:Hot Temperature; D008099:Liver / Q000166:cytology*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008297:Male; D012157:Mononuclear Phagocyte System / Q000378:metabolism*; D061848:Optical Imaging; D010587:Phagocytosis / Q000187:drug effects; D010588:Phagosomes / Q000378:metabolism*; D011713:Pyran Copolymer / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D004002:Clodronic Acid; D011713:Pyran Copolymer
keywords: In vivo phagocytosis; Kupffer cell function; mononuclear phagocyte system; phagocytosis; reticuloendothelial system