title: Effect of leaf extracts of Taraxacum officinale on CCl4 induced hepatotoxicity in rats, in vivo study.
abstract: Taraxacum officinale L is a medicinal plant, which has enormous medicinal values against various types of liver disorders and it has traditionally been used for the treatment of liver problems by people from the South East Asia. Previously we have screened the crude methanolic extract of T. officinale against cytotoxicity induced by CCl4. Present study was designed to compare the protective effect of ethanolic and n-hexane extract of leaves in carbon tetrachloride (CCl4) induced liver toxicity in rats. The extract (200 mg/kg and 400mg/kg body weight) along with silymarin (100 mg/kg) a standard drug was administered to experimental animals. It was observed that ethanolic plant extract has significantly reduced the negative effect of CCl4 as compared to n-hexane extract and effect of extract was increased with increasing dose level. Although both leaf extracts decreased the concentration of TBARS, H2O2 and nitrite contents which enhance due to CCl4 toxicity but effect was higher in ethanolic extract. The results clearly indicated that Taraxacum officinale ethanolic leaves extract has better protective effect against CCl4 induced liver tissues toxicity. This claim was also supported by histopathological results obtained during this study and this might be due to presence of various polar phytochemicals that might be more prevent in this extract.
authors.affiliation: Institute of Biochemistry and Biotechnology, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Gulfraz
authors.affiliation: Institute of Biochemistry and Biotechnology, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: Dawood
authors.initials: D
authors.lastname: Ahamd
authors.affiliation: Institute of Biochemistry and Biotechnology, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: Muhammad Sheeraz
authors.initials: MS
authors.lastname: Ahmad
authors.affiliation: Institute of Biochemistry and Biotechnology, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: Rehmatullah
authors.initials: R
authors.lastname: Qureshi
authors.affiliation: Institute of Biochemistry and Biotechnology, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
authors.forename: Raja Tahir
authors.initials: RT
authors.lastname: Mahmood
authors.affiliation: Department of Biotechnology, Islamic International University, Islamabad, Pakistan.
authors.forename: Nyla
authors.initials: N
authors.lastname: Jabeen
authors.affiliation: Department of Agriculture, University of Hairpure, KPK, Pakistan.
authors.forename: Kashif Sarfraz
authors.initials: KS
authors.lastname: Abbasi
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D031205:Taraxacum / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D002251:Carbon Tetrachloride