title: Multipotent neural crest stem cell-like cells from rat vibrissa dermal papilla induce neuronal differentiation of PC12 cells.
abstract: Bone marrow mesenchymal stem cells (BMSCs) transplants have been approved for treating central nervous system (CNS) injuries and diseases; however, their clinical applications are limited. Here, we model the therapeutic potential of dermal papilla cells (DPCs) in vitro. DPCs were isolated from rat vibrissae and characterized by immunocytofluorescence, RT-PCR, and multidifferentiation assays. We examined whether these cells could secrete neurotrophic factors (NTFs) by using cocultures of rat pheochromocytoma cells (PC12) with conditioned medium and ELISA assay. DPCs expressed Sox10, P75, Nestin, Sox9, and differentiated into adipocytes, osteoblasts, smooth muscle cells, and neurons under specific inducing conditions. The DPC-conditioned medium (DPC-CM) induced neuronal differentiation of PC12 cells and promoted neurite outgrowth. Results of ELISA assay showed that compared to BMSCs, DPCs secreted more brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF). Moreover, we observed that, compared with the total DPC population, sphere-forming DPCs expressed higher levels of Nestin and P75 and secreted greater amounts of GDNF. The DPCs from craniofacial hair follicle papilla may be a new and promising source for treating CNS injuries and diseases.
authors.affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China.
authors.forename: Meiying
authors.identifier: 0000-0003-4802-5859
authors.initials: M
authors.lastname: Li
authors.affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China.
authors.forename: Jin Yu
authors.identifier: 0000-0002-1000-9434
authors.initials: JY
authors.lastname: Liu
authors.affiliation: Department of Gynaecology, Third Hospital of Jilin University, Changchun, China.
authors.forename: Shichao
authors.identifier: 0000-0002-4111-6283
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Biological Engineering, College of Pharmacy, Jilin University, Changchun, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Gynaecology, First Hospital of Jilin University, Changchun, China.
authors.forename: Lifeng
authors.identifier: 0000-0003-0755-8333
authors.initials: L
authors.lastname: Cui
authors.affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China.
authors.forename: Shuang
authors.identifier: 0000-0002-2741-0291
authors.initials: S
authors.lastname: Lv
authors.affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China.
authors.forename: Jinying
authors.initials: J
authors.lastname: Xu
authors.affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China.
authors.forename: Shutong
authors.initials: S
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China.
authors.forename: Guangfan
authors.identifier: 0000-0001-7096-9106
authors.initials: G
authors.lastname: Chi
authors.affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 126 Xinmin Street, Changchun, Jilin 130021, China.
authors.forename: Yulin
authors.identifier: 0000-0002-3758-7568
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D018920:Coculture Techniques; D018859:Hair Follicle / Q000166:cytology; D039902:Multipotent Stem Cells / Q000166:cytology*; D009432:Neural Crest / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D009925:Organ of Corti / Q000166:cytology / Q000254:growth & development; D016716:PC12 Cells; D051381:Rats; D014738:Vibrissae / Q000166:cytology* / Q000254:growth & development