title: Highly selective fluorescence off-on probes for biothiols and imaging in live cells.
abstract: Three sulfonyl benzothiazole-based fluorescent probes (, , and ) for the detection of biothiols (cysteine, homocysteine, and glutathione) are developed based on thiol-mediated nucleophilic aromatic substitutions. The probes exhibited good selectivity and sensitivity toward biothiols over other analytes. The probes were successfully applied for visualizing endogenous thiols in living cells.
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, China. yeyong03@tsinghua.org.cn.
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Jianming
authors.initials: J
authors.lastname: Kang
authors.forename: Yong
authors.initials: Y
authors.lastname: Ye
authors.forename: Yufen
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Xian
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D002625:Chemistry, Organic / Q000379:methods; D003545:Cysteine / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D006367:HeLa Cells; D006710:Homocysteine / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D009682:Magnetic Resonance Spectroscopy; D016877:Oxidants / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D052160:Benzothiazoles; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007477:Ions; D016877:Oxidants; C000598069:RSHP3 compound; D013438:Sulfhydryl Compounds; D006710:Homocysteine; C005465:benzothiazole; D005978:Glutathione; D003545:Cysteine