title: Effects of nutritional level of concentrate-based diets on meat quality and expression levels of genes related to meat quality in Hainan black goats.
abstract: The present study investigated the effects of the nutritional levels of diets on meat quality and related gene expression in Hainan black goat. Twenty-four goats were divided into six dietary treatments and were fed a concentrate-based diet with two levels of crude protein (CP) (15% or 17%) and three levels of digestive energy (DE) (11.72, 12.55 or 13.39 MJ/kg DM) for 90 days. Goats fed the concentrate-based diet with 17% CP had significantly (P < 0.05) higher average daily gains (ADG) and better feed conversion rates (FCR). The pH 24h value tended to decrease (P < 0.05) with increasing DE levels. The tenderness of Longissimus dorsi muscle (LD) and Semimembranosus muscle (SM) reduced with increasing CP levels (P < 0.05). With increasing DE levels, tenderness was increased (P < 0.05). The heart fatty acid-binding protein (H-FABP) mRNA expression levels in LD and SM increased with increasing DE levels (P < 0.05), but decreased with increasing CP levels (P < 0.05). The calpastatin (CAST) and μ-calpain mRNA expressions levels in LD and SM were affected significantly (P < 0.05) by CP and DE levels in the diet. Therefore, the nutritional levels of diets affect meat quality and expression levels of genes associated with meat quality in Hainan black goats.
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China.
authors.forename: Dingfa
authors.initials: D
authors.lastname: Wang
authors.forename: Luli
authors.initials: L
authors.lastname: Zhou
authors.forename: Hanlin
authors.initials: H
authors.lastname: Zhou
authors.forename: Guanyu
authors.initials: G
authors.lastname: Hou
authors.forename: Liguang
authors.initials: L
authors.lastname: Shi
authors.forename: Mao
authors.initials: M
authors.lastname: Li
authors.forename: Xianzhou
authors.initials: X
authors.lastname: Huang
authors.forename: Song
authors.initials: S
authors.lastname: Guan
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D002135:Calcium-Binding Proteins / Q000235:genetics; D002154:Calpain / Q000235:genetics / Q000378:metabolism; D004044:Dietary Proteins / Q000032:analysis; D004063:Digestion / Q000502:physiology*; D004734:Energy Metabolism; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D063427:Food Quality; D015870:Gene Expression; D006041:Goats / Q000235:genetics* / Q000502:physiology*; D008460:Meat; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D009753:Nutritive Value; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D004044:Dietary Proteins; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; C033668:calpastatin; D002154:Calpain; C449002:mu-calpain
keywords: Hainan black goat; calpastatin; heart fatty acid-binding protein; meat quality; μ-calpain