title: Determination of the ruminant origin of bone particles using fluorescence in situ hybridization (FISH).
abstract: Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone identification. Despite the vast array of techniques used to decontaminate bone fragments, the isolation and determination of bone DNA content are still problematic. Within the framework of the eradication of transmissible spongiform encephalopathies (including BSE, commonly known as "mad cow disease"), a fluorescence in situ hybridization (FISH) protocol was developed. Results from the described study showed that this method can be applied directly to bones without a demineralisation step and that it allows the identification of bovine and ruminant bones even after severe processing. The results also showed that the method is independent of exogenous contamination and that it is therefore entirely appropriate for this application.
authors.affiliation: 1] Walloon Agricultural Research Centre CRA-W, Chaussée de Namur, 24, B-5030 Gembloux, Belgium [2] Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonster 20, B-42 Sart-Tilman, B-4000 Liege, Belgium [3].
authors.forename: M C
authors.initials: MC
authors.lastname: Lecrenier
authors.affiliation: 1] Walloon Agricultural Research Centre CRA-W, Chaussée de Namur, 24, B-5030 Gembloux, Belgium [2].
authors.forename: Q
authors.initials: Q
authors.lastname: Ledoux
authors.affiliation: Walloon Agricultural Research Centre CRA-W, Chaussée de Namur, 24, B-5030 Gembloux, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Berben
authors.affiliation: Walloon Agricultural Research Centre CRA-W, Chaussée de Namur, 24, B-5030 Gembloux, Belgium.
authors.forename: O
authors.initials: O
authors.lastname: Fumière
authors.affiliation: Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonster 20, B-42 Sart-Tilman, B-4000 Liege, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Saegerman
authors.affiliation: Walloon Agricultural Research Centre CRA-W, Chaussée de Namur, 24, B-5030 Gembloux, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Baeten
authors.affiliation: Walloon Agricultural Research Centre CRA-W, Chaussée de Namur, 24, B-5030 Gembloux, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Veys
mesh_terms: D000821:Animal Feed; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D004247:DNA / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence
chemical_list: D015342:DNA Probes; D004247:DNA