title: Quantitative analysis of CD3ε in a cloned canine lymphoma cell line by selected reaction monitoring assay.
abstract: We established a mass spectrometry-based quantitative method of assaying CD3ε, a component of the T-cell receptor complex. It revealed a CD3ε level of 1 mol per cell in a newly derived canine T-cell lymphoma cell line. Our results suggest that this method has sufficient sensitivity to quantify CD3ε levels in canine lymphoma cells reliably.
authors.affiliation: a Laboratory of Veterinary Pathology , School of Veterinary Medicine, Azabu University , Sagamihara , Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Kamiie
authors.forename: Naoto
authors.initials: N
authors.lastname: Shimoyama
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Aihara
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hisasue
authors.forename: Yuko
authors.initials: Y
authors.lastname: Naya
authors.forename: Kikumi
authors.initials: K
authors.lastname: Ogihara
authors.forename: Kinji
authors.initials: K
authors.lastname: Shirota
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000378:metabolism*; D045744:Cell Line, Tumor; D002999:Clone Cells / Q000378:metabolism; D004285:Dogs; D005786:Gene Expression Regulation; D008223:Lymphoma / Q000473:pathology*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D017252:CD3 Complex
keywords: CD3; lymphoma; mass spectrometry; quantitative proteomics; selected reaction monitoring