title: Construction of a novel secretion expression system guided by native signal peptide of PhoD in Zymomonas mobilis.
abstract: In the current study, three native signal peptides (SPs) from PhoC, PhoD, and ZMO0331were investigated and compared to construct novel secretion expression systems in Zymomonas mobilis. The secretion expression of target protein, α-amylase from Bacillus amyloliquefaciens (BAA), guided by PhoD's SP resulted in more hydrolysis of starch than that by the other two SPs. Extracellular and intracellular α-amylase activities of the strain containing PhoD's SP were also higher than the other two strains containing PhoC or ZMO0331's SP. In addition, the evidence by alcohol dehydrogenase activity assay further confirmed that the starch hydrolysis was resulted from the secretion expression of BAA rather than the breakage of cells. Our results indicated that the SP of PhoD is able to serve as a promising candidate to assist secretion expression of heterogeneous genes in Z. mobilis. This will contribute to development of engineered Z. mobilis strains converting starch into ethanol.
authors.affiliation: a College of Life Sciences , Sichuan University , Chengdu , China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Ming-Xiong
authors.initials: MX
authors.lastname: He
authors.forename: Hong
authors.initials: H
authors.lastname: Feng
authors.forename: Zong-Xia
authors.initials: ZX
authors.lastname: Shui
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Tang
authors.forename: Qi-Chun
authors.initials: QC
authors.lastname: Hu
authors.forename: Yi-Zheng
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology; D003001:Cloning, Molecular; D005285:Fermentation; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006868:Hydrolysis; D027723:Ipomoea batatas / Q000737:chemistry; D021382:Protein Sorting Signals / Q000235:genetics*; D017421:Sequence Analysis; D013213:Starch / Q000378:metabolism; D017355:Zymomonas / Q000235:genetics* / Q000378:metabolism; D000516:alpha-Amylases / Q000235:genetics
chemical_list: D021382:Protein Sorting Signals; D013213:Starch; D000426:Alcohol Dehydrogenase; D000469:Alkaline Phosphatase; D000516:alpha-Amylases
keywords: Zymomonas mobilis; consolidated bioprocessing; secretion expression; signal peptide; α-amylase