title: Sequence-specific DNA interactions with calixarene-based langmuir monolayers.
abstract: The interactions of an amphiphilic calixarene, namely p-guanidino-dodecyloxy-calix[4]arene, 1, self-assembled as Langmuir monolayers, with short double stranded DNA, were investigated by surface pressure-area (π-A) isotherms, surface ellipsometry and Brewster angle microscopy (BAM). Three DNA 30mers were used as models, poly(AT), poly(GC) and a random DNA sequence with 50% of G:C base pairs. The interactions of these model DNA duplexes with 1-based Langmuir monolayers were studied by measuring compression isotherms using increasing DNA concentrations (10(-6), 10(-5), 10(-4), and 5 × 10(-4) g L(-1)) in the aqueous subphase. The isotherms of 1 showed an expansion of the monolayer with, interestingly, significant differences depending on the duplex DNA sequence studied. Indeed, the interactions of 1-based monolayers with poly(AT) led to an expansion of the monolayer that was significantly more pronounced that for monolayers on subphases of poly(GC) and the random DNA sequence. The structure and thickness of 1-based Langmuir monolayers were investigated by BAM and surface ellipsometry that showed differences in thickness and structure between a monolayer formed on pure water or on a DNA subphase, with here again relevant dissimilarities depending on the DNA composition.
authors.affiliation: Institute of Chemistry and Bioanalytics, University of Applied Sciences and Arts Northwestern Switzerland , Gründenstrasse 40, CH-4132 Muttenz, Switzerland.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Rullaud
authors.forename: Negar
authors.initials: N
authors.lastname: Moridi
authors.forename: Patrick
authors.initials: P
authors.lastname: Shahgaldian
mesh_terms: D000388:Air / Q000032:analysis; D001482:Base Composition; D001483:Base Sequence; D047250:Calixarenes / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007700:Kinetics; D008969:Molecular Sequence Data; D011312:Pressure; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D047250:Calixarenes; D004247:DNA