title: A 9 kDa antifreeze protein from the Antarctic springtail, Gomphiocephalus hodgsoni.
abstract: A 9 kDA antifreeze protein (AFP) was isolated and purified from the Antarctic springtail, Gomphiocephalus hodgsoni. By combining selective sampling procedures and a modified ice affinity purification protocol it was possible to directly isolate a single AFP protein without recourse to chromatographic separation techniques. Mass spectrometry identified a single 9 kDa component in the purified ice fraction. Intramolecular disulphide bonding was suggested by the presence of 12 cysteine residues. The specific amino acid composition is unique, particularly with regard to the presence of histidine (11.5%). But it also shows noticeable commonalities with insect AFPs in the abundance of cysteine (13.8%), while simultaneously hinting, through the presence of glycine (11.5%), that the metabolic building blocks of AFPs in Collembola may have a phylogenetically-determined component.
authors.affiliation: Asian University, Banglamung, Chonburi 20260, Thailand; Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand. Electronic address: timothyhawes@hotmail.com.
authors.forename: T C
authors.initials: TC
authors.lastname: Hawes
authors.affiliation: Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand.
authors.forename: C J
authors.initials: CJ
authors.lastname: Marshall
authors.affiliation: Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand.
authors.forename: D A
authors.initials: DA
authors.lastname: Wharton
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D000864:Antarctic Regions; D021301:Antifreeze Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D001181:Arthropods; D003080:Cold Temperature; D007053:Ice / Q000009:adverse effects; D013058:Mass Spectrometry; D017433:Protein Structure, Secondary
chemical_list: D021301:Antifreeze Proteins; D007053:Ice
keywords: Amino acid composition; Antifreeze protein; Collembola; Ice-binding; Springtail