title: Replication-associated proteins encoded by Wheat dwarf virus act as RNA silencing suppressors.
abstract: Wheat dwarf virus (WDV) is an economically important and widespread single-stranded DNA virus in the genus Mastrevirus, family Geminiviridae. So far, an RNA silencing suppressor in the genus Mastrevirus has not been reported. In this study, the viral replication-associated proteins of WDV, Rep and RepA proteins, were demonstrated to have RNA silencing suppressor activities when expressed in agro-infiltrated leaves of Nicotiana benthamiana line 16c, by their ability to inhibit posttranscriptional gene silencing (PTGS) of green fluorescent protein (GFP). On the basis of the analysis of GFP expression and siRNA accumulation, we concluded that both Rep and RepA proteins suppressed local silencing induced by either single-stranded GFP (ssGFP) or inverted repeat GFP (dsGFP) constructs, although their activity can be characterized as relatively weak in comparison to HC-Pro of tobacco etch virus.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuan Ming Yuan Road, Beijing 100193, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuan Ming Yuan Road, Beijing 100193, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Jin
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuan Ming Yuan Road, Beijing 100193, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuan Ming Yuan Road, Beijing 100193, China. Electronic address: wangxifeng@caas.cn.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D017791:Geminiviridae / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D012097:Repressor Proteins; D014764:Viral Proteins
keywords: Mastrevirus; PTGS; RNA silencing suppressor; Wheat dwarf virus