title: A prepared anti-MSTN polyclonal antibody reverses insulin resistance of diet-induced obese rats via regulation of PI3K/Akt/mTOR&FoxO1 signal pathways.
abstract: Suppression of myostatin (MSTN) is associated with skeletal muscle atrophy and insulin resistance. However, the mechanisms by which MSTN regulates insulin resistance are not well known. We have explored the signaling pathways through which MSTN regulates insulin resistance in diet-induced obese rats using a polyclonal antibody for MSTN. The anti-MSTN polyclonal antibody significantly improved insulin resistance and whole-body insulin sensitivity, decreased MSTN protein expression in muscle samples by 39% in diet-induced obese rats. Furthermore, the anti-MSTN polyclonal antibody significantly enhanced PI3K activity (140%), Akt phosphorylation (86%), GLUT4 protein expression (23%), the phosphorylation of mTOR (21%), and inhibited the phosphorylation of FoxO1 (57%), but did not affect the phosphorylation of GSK-3β. Thus, suppression of MSTN by the anti-MSTN polyclonal antibody reverses insulin resistance of diet-induced obesity via MSTN/PI3K/Akt/mTOR and MSTN/PI3K/Akt/FoxO1 signaling pathways.
authors.affiliation: Institute of Sports Biology, Shaanxi Normal University, Xi'an, 710062, China, tl531@snnu.edu.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Tang
authors.forename: Chen-tao
authors.initials: CT
authors.lastname: Liu
authors.forename: Xu-dan
authors.initials: XD
authors.lastname: Wang
authors.forename: Kai
authors.initials: K
authors.lastname: Luo
authors.forename: Di-di
authors.initials: DD
authors.lastname: Zhang
authors.forename: Ai-ping
authors.initials: AP
authors.lastname: Chi
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Sun
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000008:administration & dosage*; D004195:Disease Models, Animal; D007333:Insulin Resistance; D055435:Myostatin / Q000037:antagonists & inhibitors* / Q000276:immunology; D009765:Obesity / Q000150:complications*; D051381:Rats; D015398:Signal Transduction
chemical_list: D001323:Autoantibodies; C525913:Mstn protein, rat; D055435:Myostatin