title: Combination of grape seed proanthocyanidin extract and docosahexaenoic acid-rich oil increases the hepatic detoxification by GST mediated GSH conjugation in a lipidic postprandial state.
abstract: The ingestion of dietary lipids leads to oxidative stress. This postprandial oxidative stress may potentiate the adverse effects of postprandial hyperlipidaemia. Proanthocyanidins have been shown to alleviate oxidative stress and hypertriglyceridaemia associated with the postprandial state. Additionally, omega-3 polyunsaturated fatty acids (PUFAs) also have beneficial effects on lipoprotein metabolism and oxidative stress. The present study was designed to investigate the possible additive effects in liver of an acute dose of grape seed proanthocyanidins extract (GSPE) and oil rich in docosahexaenoic acid (DHA-OR) on lipidic postprandial oxidative stress in Wistar rats. GSPE+DHA-OR modifies the hepatic antioxidant enzymatic activities (GST and GPx), clearly showing that this combination increases the detoxification of postprandial xenobiotics via the GST action mediated hepatic GSH conjugation. In conclusion, this study provides evidence that the combination of GSPE and DHA-OR ameliorate the transient imbalance between the lipid hydroperoxide level and antioxidant status related to a lipidic postprandial state.
authors.affiliation: Grup de Nutrigenòmica, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Campus Sescel·lades, 43007 Tarragona, Spain.
authors.forename: Anabel
authors.initials: A
authors.lastname: Fernández-Iglesias
authors.affiliation: Grup de Nutrigenòmica, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Campus Sescel·lades, 43007 Tarragona, Spain.
authors.forename: Helena
authors.initials: H
authors.lastname: Quesada
authors.affiliation: Grup de Nutrigenòmica, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Campus Sescel·lades, 43007 Tarragona, Spain.
authors.forename: Sabina
authors.initials: S
authors.lastname: Díaz
authors.affiliation: Grup de Nutrigenòmica, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Campus Sescel·lades, 43007 Tarragona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Pajuelo
authors.affiliation: Grup de Nutrigenòmica, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Campus Sescel·lades, 43007 Tarragona, Spain.
authors.forename: Cinta
authors.initials: C
authors.lastname: Bladé
authors.affiliation: Grup de Nutrigenòmica, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Campus Sescel·lades, 43007 Tarragona, Spain.
authors.forename: Lluís
authors.initials: L
authors.lastname: Arola
authors.affiliation: Grup de Nutrigenòmica, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Campus Sescel·lades, 43007 Tarragona, Spain.
authors.forename: M Josepa
authors.initials: MJ
authors.lastname: Salvadó
authors.affiliation: Grup de Nutrigenòmica, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Campus Sescel·lades, 43007 Tarragona, Spain. Electronic address: miquel.mulero@urv.cat.
authors.forename: Miquel
authors.initials: M
authors.lastname: Mulero
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D056604:Grape Seed Extract / Q000737:chemistry*; D008099:Liver / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D019518:Postprandial Period; D044945:Proanthocyanidins / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D044945:Proanthocyanidins; D004281:Docosahexaenoic Acids
keywords: GSH; GST; Oxidative stress; PUFAs omega-3; Postprandial; Proanthocyanidins