title: Genetic diversity and structure of natural Dactylis glomerata L. populations revealed by morphological and microsatellite-based (SSR/ISSR) markers.
abstract: Dactylis glomerata L. is an important forage species in the Mediterranean region, and in other regions with a similar climate. Genetic material from 3 locations in north, central, and south Greece was studied, using morphological traits, SSR, and ISSR molecular markers. Morphological analysis revealed differences among the geographic locations studied for all morphological traits, except the number of reproductive tillers. Moreover, the highest phenotypic variation was observed on the accessions from south, while the lowest was observed on the accessions from the north. Although the results of the molecular marker analysis are indicative, a high level of genetic diversity at the species level was revealed by ISSRs (GST=0.291) and SSRs (FST=0.186). Analysis of molecular variance showed that a high level of genetic diversity existed for ISSRs and SSRs within populations (62 and 83%, respectively), rather than among populations (38 and 17%, respectively). Cluster analysis divided the 3 populations in 2 groups, with the population originating from the island of Crete forming 1 group, while the populations from north Greece (Taxiarchis) and central Greece (Pertouli) were clustered in a 2nd group. In general, the results indicate that SSRs are more informative compared to ISSRs about the genetic variation within a population, whereas the ISSRs were more informative about the genetic diversity among populations However, a similar trend in diversity (genotypic and phenotypic) was observed in the morphological traits and microsatellite-based (SSR/ISSR) markers at the locations studied.
authors.affiliation: Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece.
authors.forename: P
authors.initials: P
authors.lastname: Madesis
authors.affiliation: Laboratory of Range Science, Department of Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, Greece eabraham@for.auth.gr.
authors.forename: E M
authors.initials: EM
authors.lastname: Abraham
authors.affiliation: Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Kalivas
authors.affiliation: Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece.
authors.forename: Iota
authors.initials: I
authors.lastname: Ganopoulos
authors.affiliation: Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Tsaftaris
mesh_terms: D000704:Analysis of Variance; D016000:Cluster Analysis; D031745:Dactylis / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D018745:Genome, Plant; D018895:Microsatellite Repeats; D025341:Principal Component Analysis; D012639:Seeds / Q000235:genetics*