title: Characterization and evaluation of 5-fluorouracil-loaded solid lipid nanoparticles prepared via a temperature-modulated solidification technique.
abstract: The aim of this research was to advance solid lipid nanoparticle (SLN) preparation methodology by preparing glyceryl monostearate (GMS) nanoparticles using a temperature-modulated solidification process. The technique was reproducible and prepared nanoparticles without the need of organic solvents. An anticancer agent, 5-fluorouracil (5-FU), was incorporated in the SLNs. The SLNs were characterized by particle size analysis, zeta potential analysis, differential scanning calorimetry (DSC), infrared spectroscopy, atomic force microscopy (AFM), transmission electron microscopy (TEM), drug encapsulation efficiency, in vitro drug release, and in vitro cell viability studies. Particle size of the SLN dispersion was below 100 nm, and that of redispersed lyophilizates was ~500 nm. DSC and infrared spectroscopy suggested that the degree of crystallinity did not decrease appreciably when compared to GMS. TEM and AFM images showed well-defined spherical to oval particles. The drug encapsulation efficiency was found to be approximately 46%. In vitro drug release studies showed that 80% of the encapsulated drug was released within 1 h. In vitro cell cultures were biocompatible with blank SLNs but demonstrated concentration-dependent changes in cell viability to 5-FU-loaded SLNs. The 5-FU-loaded SLNs can potentially be utilized in an anticancer drug delivery system.
authors.affiliation: Department of Pharmacy Practice, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, 3000 Arlington Avenue, MS1013, Toledo, Ohio, 43614, USA.
authors.forename: Meghavi N
authors.initials: MN
authors.lastname: Patel
authors.forename: Sushant
authors.initials: S
authors.lastname: Lakkadwala
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Majrad
authors.forename: Elisha R
authors.initials: ER
authors.lastname: Injeti
authors.forename: Steven M
authors.initials: SM
authors.lastname: Gollmer
authors.forename: Zahoor A
authors.initials: ZA
authors.lastname: Shah
authors.forename: Sai Hanuman Sagar
authors.initials: SH
authors.lastname: Boddu
authors.forename: Jerry
authors.initials: J
authors.lastname: Nesamony
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000737:chemistry* / Q000494:pharmacology; D018938:Caco-2 Cells; D002152:Calorimetry, Differential Scanning; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D005472:Fluorouracil / Q000737:chemistry*; D005989:Glycerides / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D036103:Nanotechnology; D010316:Particle Size; D044367:Phase Transition; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature
chemical_list: D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; D005989:Glycerides; C048159:glyceryl monostearate; D005472:Fluorouracil