title: Evaluation of two point-of-care ethylene glycol tests for dogs.
abstract: OBJECTIVE
  To evaluate 2 point-of-care ethylene glycol (EG) tests in dogs.
  
  
  METHODS
  Prospective, randomized, blinded laboratory evaluation.
  
  
  METHODS
  University teaching hospital.
  
  
  METHODS
  Ten healthy adult dogs.
  
  
  METHODS
  Jugular venipuncture and in vitro evaluation for detection of EG in canine blood.
  
  
  METHODS
  Whole blood samples were centrifuged and separated, and the plasma was divided into 30 aliquots. The aliquots were mixed with EG to provide EG concentrations ranging from 0 to 100 mg/dL. The EG concentration of each sample was confirmed using gas chromatography. For the VetSpec EG Qualitative Reagent Test Kit, 100 μL of each sample was added to test vials and compared with 20 and 50 mg/dL reference vials. For the Kacey EG Test Strips, 20 μL of each sample was added to the test circle and compared with the color chart provided by the manufacturer. For each test, samples were prepared in groups of 5 and presented in randomized order to 2 readers who were blinded to the presumed EG concentration. Samples were scored as negative, 20-50 mg/dL, or greater than 50 mg/dL. For each test, the sensitivity and specificity for detecting EG was calculated. Cohen's unweighted kappa coefficient was calculated to determine the degree of agreement between readers.
  
  
  RESULTS
  For detecting EG, the Kacey EG Test Strips had excellent sensitivity and specificity (both 100%) and good agreement between readers. The VetSpec EG Qualitative Reagent Test Kit was less sensitive and specific (65% and 70% for the first reader, 95% and 40% for the second) with less agreement.
  
  
  CONCLUSIONS
  Of the 2 systems evaluated, the Kacey EG Test Strips displayed greater accuracy and ease of use.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI, 48824.
authors.forename: Karina J
authors.initials: KJ
authors.lastname: Creighton
authors.forename: Amy M
authors.initials: AM
authors.lastname: Koenigshof
authors.forename: Christian D
authors.initials: CD
authors.lastname: Weder
authors.forename: L Ari
authors.initials: LA
authors.lastname: Jutkowitz
mesh_terms: D000818:Animals; D003124:Colorimetry / Q000379:methods; D004285:Dogs / Q000097:blood*; D019855:Ethylene Glycol / Q000097:blood*; D019095:Point-of-Care Systems; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic; D019855:Ethylene Glycol
keywords: antifreeze; canine; point-of-care systems; toxicology