title: Spatiotemporal intracellular nitric oxide signaling captured using internalized, near-infrared fluorescent carbon nanotube nanosensors.
abstract: Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
authors.forename: Zachary W
authors.initials: ZW
authors.lastname: Ulissi
authors.forename: Fatih
authors.initials: F
authors.lastname: Sen
authors.forename: Xun
authors.initials: X
authors.lastname: Gong
authors.forename: Selda
authors.initials: S
authors.lastname: Sen
authors.forename: Nicole
authors.initials: N
authors.lastname: Iverson
authors.forename: Ardemis A
authors.initials: AA
authors.lastname: Boghossian
authors.forename: Luiz C
authors.initials: LC
authors.lastname: Godoy
authors.forename: Gerald N
authors.initials: GN
authors.lastname: Wogan
authors.forename: Debabrata
authors.initials: D
authors.lastname: Mukhopadhyay
authors.forename: Michael S
authors.initials: MS
authors.lastname: Strano
mesh_terms: D045744:Cell Line, Tumor; D005453:Fluorescence; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009569:Nitric Oxide / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D037742:Nanotubes, Carbon; D009569:Nitric Oxide