title: In vivo studies: comparing the administration via and the impact on the biodistribution of radiopharmaceuticals.
abstract: The use of in vivo assay to determine the biodistribution and subsequent inter-comparison with human parameters has been used since the dawn of science. The use of this type of test admits the metabolic equity among animals for inter-comparison. Thus, the use of Wistar rats in particular is quite frequent. Regarding routes of administration, there are three ways to test priority: jugular vein, intraocular (eye plexus) and caudal; there is a consensus that these three pathways behave in the same way, or at least very similar. Biodistribution studies of drugs, especially radiopharmaceuticals, have been using randomly any of these pathways believed to be effective in their likeness without worrying about your real analytic equity. In this study, we performed in vivo assay in 8 Wistar rats using 99mTc -labeled Herceptin to review the route of administration on the biodistribution result. Thus, four mice were injected via the intraocular (eye plexus), and four were injected via tail (caudal plexus). The results were quite disparate and call the attention of the scientific community to reassess the protocols for animal experiments, in order to have uniformity and fairness between the data and may represent a test for human inter-comparison of more reliable and trustworthy way.
authors.affiliation: Zona Oeste Estadual University, Laboratory of Radiopharmacy, Rio de Janeiro, Brazil.
authors.forename: Suyene Rocha
authors.initials: SR
authors.lastname: Pinto
authors.affiliation: Laboratory of Nanoradiopharmaceuticals, Brazilian Association of Radiopharmacy, Rio de Janeiro, Brazil.
authors.forename: Michelle Alvares
authors.initials: MA
authors.lastname: Sarcinelle
authors.affiliation: Laboratory of Nanoradiopharmaceuticals, Brazilian Association of Radiopharmacy, Rio de Janeiro, Brazil.
authors.forename: Marta
authors.initials: M
authors.lastname: de Souza Albernaz
authors.affiliation: Laboratory of Nanoradiopharmaceuticals, Brazilian Association of Radiopharmacy, Rio de Janeiro, Brazil.
authors.forename: Franciana Maria Rosa
authors.initials: FM
authors.lastname: da Silva
authors.affiliation: Zona Oeste Estadual University, Laboratory of Celullar Biology, Rio de Janeiro, Brazil.
authors.forename: Sergio Henrique
authors.initials: SH
authors.lastname: Seabra
authors.affiliation: Zona Oeste Estadual University, Vivarium Coordination, Rio de Janeiro, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Almeida do Nascimento
authors.affiliation: Zona Oeste Estadual University, Vivarium Coordination, Rio de Janeiro, Brazil.
authors.forename: Cosme Leonardo Gomes
authors.initials: CL
authors.lastname: Carvalho
authors.affiliation: Zona Oeste Estadual University, Laboratory of Radiopharmacy, Rio de Janeiro, Brazil; Laboratory of Nanoradiopharmaceuticals, Brazilian Association of Radiopharmacy, Rio de Janeiro, Brazil. Electronic address: ralpholiveira@uezo.rj.gov.br.
authors.forename: Ralph
authors.initials: R
authors.lastname: Santos-Oliveira
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D001681:Biological Assay / Q000379:methods*; D005260:Female; D008297:Male; D008657:Metabolic Clearance Rate; D009928:Organ Specificity; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013667:Technetium / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D000068878:Trastuzumab
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D019275:Radiopharmaceuticals; D013667:Technetium; D000068878:Trastuzumab
keywords: Animal studies; Biodistribution; Monoclonal antibodies; Radiopharmacy