title: Asphodosides A-E, anti-MRSA metabolites from Asphodelus microcarpus.
abstract: Bioassay guided fractionation of the ethanolic extract of Asphodelus microcarpus Salzm. et Viv. (Xanthorrhoeaceae or Asphodelaceae) resulted in isolation of five compounds identified as asphodosides A-E (1-5). Compounds 2-4 showed activity against methicillin resistant Staphylococcus aureus (MRSA) with IC50 values of 1.62, 7.0 and 9.0μg/mL, respectively. They also exhibited activity against Staphylococcus aureus (non-MRSA) with IC50 values of 1.0, 3.4 and 2.2μg/mL, respectively. The structure elucidation of isolated metabolites was carried out using spectroscopic data (1D and 2D NMR), optical rotation and both experimental and calculated electronic circular dichroism (ECD).
authors.affiliation: National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA; Department of Pharmacognosy, Faculty of Pharmacy, The University of Al-Azhar, Cairo 11371, Egypt.
authors.forename: Mohammed M
authors.initials: MM
authors.lastname: Ghoneim
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, The University of Mississippi, University, MS 38677, USA.
authors.forename: Khaled M
authors.initials: KM
authors.lastname: Elokely
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, The University of Al-Azhar, Cairo 11371, Egypt.
authors.forename: Atef A
authors.initials: AA
authors.lastname: El-Hela
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, The University of Al-Azhar, Cairo 11371, Egypt.
authors.forename: Abd-Elsalam I
authors.initials: AE
authors.lastname: Mohammad
authors.affiliation: National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Jacob
authors.affiliation: National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA; Department of Pharmacognosy, Faculty of Pharmacy, The University of Alexandria, Alexandria 21521, Egypt.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Radwan
authors.affiliation: National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA; Department of Medicinal Chemistry, School of Pharmacy, The University of Mississippi, University, MS 38677, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Doerksen
authors.affiliation: National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA; Department of Medicinal Chemistry, School of Pharmacy, The University of Mississippi, University, MS 38677, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Cutler
authors.affiliation: National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA; Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA. Electronic address: sross@olemiss.edu.
authors.forename: Samir A
authors.initials: SA
authors.lastname: Ross
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002942:Circular Dichroism; D024881:Drug Resistance, Bacterial; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019668:Liliaceae / Q000737:chemistry; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D000900:Anti-Bacterial Agents; D006576:Heterocyclic Compounds, 4 or More Rings
keywords: Asphodelaceae; Asphodelus microcarpus; Electronic circular dichroism; MRSA