title: Genotypes and antibiotic resistances of Campylobacter jejuni isolates from cattle and pigeons in dairy farms.
abstract: Campylobacter jejuni is the most common food-borne zoonotic pathogen causing human gastroenteritis worldwide and has assumed more importance in Italy following the increased consumption of raw milk. Our objectives were to get an overview of genotypes and antibiotic resistances in C. jejuni isolated from milk, cattle feces, and pigeons in dairy herds of Northern Italy. flaB-typing was applied to 78 C. jejuni isolates, previously characterized by Multi-Locus Sequence Typing, and genotypic resistances towards macrolides and quinolones based on point mutations in the 23S rRNA and gyrA genes, respectively, were determined. flaB-typing revealed 22 different types with one of them being novel and was useful to further differentiate strains with an identical Sequence Type (ST) and to identify a pigeon-specific clone. Macrolide resistance was not found, while quinolone resistance was detected in 23.3% of isolates. A relationship between specific genotypes and antibiotic resistance was observed, but was only significant for the Clonal Complex 206. Our data confirm that pigeons do not play a role in the spread of C. jejuni among cattle and they are not responsible for milk contamination. A relevant number of bulk milk samples were contaminated by C. jejuni resistant to quinolones, representing a possible source of human resistant strains.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Lodi 26900, Italy. bianchini.valentina87@gmail.com.
authors.forename: Valentina
authors.initials: V
authors.lastname: Bianchini
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Lodi 26900, Italy. mariovittorio.luini@izsler.it.
authors.forename: Mario
authors.initials: M
authors.lastname: Luini
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Lodi 26900, Italy. laurabl83@gmail.com.
authors.forename: Laura
authors.initials: L
authors.lastname: Borella
authors.affiliation: Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Putignano (BA) 70017, Italy. parisi.izspb@gmail.com.
authors.forename: Antonio
authors.initials: A
authors.lastname: Parisi
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern CH-3001, Switzerland. r.jonas987@gmail.com.
authors.forename: Romie
authors.initials: R
authors.lastname: Jonas
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern CH-3001, Switzerland. sonja.kittl@vetsuisse.unibe.ch.
authors.forename: Sonja
authors.initials: S
authors.lastname: Kittl
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern CH-3001, Switzerland. peter.kuhnert@vetsuisse.unibe.ch.
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhnert
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle / Q000382:microbiology*; D010856:Columbidae / Q000382:microbiology*; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D003612:Dairying; D024881:Drug Resistance, Bacterial; D004784:Environmental Monitoring; D005243:Feces / Q000382:microbiology*; D005408:Flagellin / Q000235:genetics; D005838:Genotype; D007558:Italy; D018942:Macrolides / Q000494:pharmacology; D008892:Milk / Q000382:microbiology*; D058885:Multilocus Sequence Typing; D015363:Quinolones / Q000494:pharmacology; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides; D015363:Quinolones; D012338:RNA, Ribosomal, 23S; D005408:Flagellin; C416532:flaB flagellin; D027081:DNA Gyrase