title: Cloning and characterization of the mouse JDP2 gene promoter reveal negative regulation by p53.
abstract: Jun dimerization protein 2 (JDP2) is a repressor of transcription factor AP-1. To investigate the transcriptional regulation of the JDP2 gene, we cloned the 5'-flanking region of the mouse JDP2 gene. Primer extension analysis revealed a new transcription start site (+1). Promoter analysis showed that the region from nt -343 to nt +177 contains basal transcriptional activity. Interestingly, the tumor suppressor p53 significantly repressed the transcriptional activity of the JDP2 promoter. Given that JDP2 inhibits expression of p53, our results suggest a negative feedback loop between JDP2 and p53, and a direct link between JDP2 and a key oncogenic pathway.
authors.affiliation: Department of Pancreatic Gastroenterologic Surgery, First Hospital of China Medical University, No. 92, Nanjing Rd, Shenyang 110001, China. Electronic address: xuyhsy@yeah.net.
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Rd, Tuxedo Park, NY 10987, USA.
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Jin
authors.affiliation: Department of Pancreatic Gastroenterologic Surgery, First Hospital of China Medical University, No. 92, Nanjing Rd, Shenyang 110001, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, China.
authors.forename: Jianzhi
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Rd, Tuxedo Park, NY 10987, USA.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pancreatic Gastroenterologic Surgery, First Hospital of China Medical University, No. 92, Nanjing Rd, Shenyang 110001, China.
authors.forename: Zhongbo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Pancreatic Gastroenterologic Surgery, First Hospital of China Medical University, No. 92, Nanjing Rd, Shenyang 110001, China.
authors.forename: Shulong
authors.initials: S
authors.lastname: Bi
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, 100 Shih-Chuan 1st Rd, San-Ming District, Kaohsiung 807, Taiwan. Electronic address: kazu@kmu.edu.tw.
authors.forename: Kazunari K
authors.initials: KK
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*
chemical_list: C491386:Jundp2 protein, mouse; D012097:Repressor Proteins; D016159:Tumor Suppressor Protein p53; D004247:DNA
keywords: JDP2; Promoter; Transcriptional regulation; p53