title: Characterization and potential use of cuttlefish skin gelatin hydrolysates prepared by different microbial proteases.
abstract: Composition, functional properties, and in vitro antioxidant activities of gelatin hydrolysates prepared from cuttlefish skin were investigated. Cuttlefish skin gelatin hydrolysates (CSGHs) were obtained by treatment with crude enzyme preparations from Bacillus licheniformis NH1, Bacillus mojavensis A21, Bacillus subtilis A26, and commercial alcalase. All CSGHs had high protein contents, 74.3-78.3%, and showed excellent solubility (over 90%). CSGH obtained by alcalase demonstrated high antioxidant activities monitored by β-carotene bleaching, DPPH radical scavenging, lipid peroxidation inhibition, and reducing power activity. Its antioxidant activity remained stable or increased in a wide range of pH (1-9), during heating treatment (100°C for 240 min) and after gastrointestinal digestion simulation. In addition, alcalase-CSGH was incorporated into turkey meat sausage to determine its effect on lipid oxidation during 35 days of storage period. At 0.5 mg/g, alcalase-CSGH delayed lipid oxidation monitored by TBARS and conjugated diene up to 10 days compared to vitamin C. The results reveal that CSGHs could be used as food additives possessing both antioxidant activity and functional properties.
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
authors.forename: Mourad
authors.initials: M
authors.lastname: Jridi
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Lassoued
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Nasri
authors.affiliation: Laboratoire d'Analyse Alimentaire, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
authors.forename: Mohamed Ali
authors.initials: MA
authors.lastname: Ayadi
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
authors.affiliation: Laboratoire de Biodiversité et de Biotechnologie Marine, Centre de Sfax, Institut National des Sciences et Technologies de la Mer, B.P. 1037, 3018 Sfax, Tunisia.
authors.forename: Nabil
authors.identifier: 0000-0001-6244-1395
authors.initials: N
authors.lastname: Souissi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D001407:Bacillus / Q000201:enzymology*; D049832:Decapodiformes / Q000737:chemistry; D005780:Gelatin / Q000008:administration & dosage* / Q000737:chemistry; D006868:Hydrolysis; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D010447:Peptide Hydrolases / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000008:administration & dosage / Q000737:chemistry; D012867:Skin / Q000737:chemistry
chemical_list: D000975:Antioxidants; D011492:Protein Hydrolysates; D005780:Gelatin; D010447:Peptide Hydrolases