title: Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars.
abstract: Classical swine fever is one of the most important infectious diseases for the pig industry worldwide due to its economic impact. Vaccination is an effective means to control disease, however within the EU its regular use is banned owing to the inability to differentiate infected and vaccinated animals, the so called DIVA principle. This inability complicates monitoring of disease and stops international trade thereby limiting use of the vaccine in many regions. The C-strain vaccine is safe to use and gives good protection. It is licensed for emergency vaccination in the EU in event of an outbreak. Two genetic assays that can distinguish between wild type virus and C-strain vaccines have recently been developed. Here the results from a comparison of these two real-time RT-PCR assays in an interlaboratory exercise are presented. Both assays showed similar performance.
authors.affiliation: Department of Virology, Immunobiology and Parasitology, The National Veterinary Institute (SVA), Uppsala, Sweden. Electronic address: frederik.widen@sva.se.
authors.forename: F
authors.initials: F
authors.lastname: Widén
authors.affiliation: Virology Department, Animal Health and Veterinary Laboratories Agency (AHVLA), Addlestone, UK.
authors.forename: H
authors.initials: H
authors.lastname: Everett
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), Greifswald-Insel Riems, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Blome
authors.affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA), Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Fernandez Pinero
authors.affiliation: National Veterinary Institute, Technical University of Denmark (DTU-VET), Lindholm, Kalvehave, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Uttenthal
authors.affiliation: Department of Virology, Immunobiology and Parasitology, The National Veterinary Institute (SVA), Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Cortey
authors.affiliation: National Veterinary Institute, Technical University of Denmark (DTU-VET), Lindholm, Kalvehave, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: von Rosen
authors.affiliation: Veterinary and Agrochemical Research Centre CODA-CERVA, Uccle, Brussel, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Tignon
authors.affiliation: Department of Virology, Immunobiology and Parasitology, The National Veterinary Institute (SVA), Uppsala, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology; D006691:Classical Swine Fever / Q000175:diagnosis* / Q000276:immunology*; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000276:immunology; D004279:DNA, Viral / Q000235:genetics; D003937:Diagnosis, Differential; D003955:Diagnostic Tests, Routine / Q000662:veterinary; D005062:European Union; D005838:Genotype; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D034421:Sus scrofa / Q000821:virology*; D013552:Swine / Q000821:virology*; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000145:classification / Q000235:genetics* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D014765:Viral Vaccines
keywords: C-strain; CSFV; Classical swine fever; DIVA; PCR; Vaccine