title: Enterovirus71 (EV71) utilise host microRNAs to mediate host immune system enhancing survival during infection.
abstract: Hand, Foot and Mouth Disease (HFMD) is a self-limiting viral disease that mainly affects infants and children. In contrast with other HFMD causing enteroviruses, Enterovirus71 (EV71) has commonly been associated with severe clinical manifestation leading to death. Currently, due to a lack in understanding of EV71 pathogenesis, there is no antiviral therapeutics for the treatment of HFMD patients. Therefore the need to better understand the mechanism of EV71 pathogenesis is warranted. We have previously reported a human colorectal adenocarcinoma cell line (HT29) based model to study the pathogenesis of EV71. Using this system, we showed that knockdown of DGCR8, an essential cofactor for microRNAs biogenesis resulted in a reduction of EV71 replication. We also demonstrated that there are miRNAs changes during EV71 pathogenesis and EV71 utilise host miRNAs to attenuate antiviral pathways during infection. Together, data from this study provide critical information on the role of miRNAs during EV71 infection.
authors.affiliation: School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia; School of Chemical and Life Sciences, Singapore Polytechnic, Singapore, Singapore; Centre for Biomedical and Life Sciences, Singapore Polytechnic, Singapore, Singapore.
authors.forename: Yan Long Edmund
authors.initials: YL
authors.lastname: Lui
authors.affiliation: School of Chemical and Life Sciences, Singapore Polytechnic, Singapore, Singapore.
authors.forename: Tuan Lin
authors.initials: TL
authors.lastname: Tan
authors.affiliation: School of Chemical and Life Sciences, Singapore Polytechnic, Singapore, Singapore.
authors.forename: Wee Hong
authors.initials: WH
authors.lastname: Woo
authors.affiliation: School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia; Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sippy Downs, Queensland, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Timms
authors.affiliation: School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
authors.forename: Louise Marie
authors.initials: LM
authors.lastname: Hafner
authors.affiliation: School of Chemical and Life Sciences, Singapore Polytechnic, Singapore, Singapore.
authors.forename: Kian Hwa
authors.initials: KH
authors.lastname: Tan
authors.affiliation: Centre for Biomedical and Life Sciences, Singapore Polytechnic, Singapore, Singapore; Department of Paediatrics, University Children's Medical Institute, National University Hospital, Singapore, Singapore.
authors.forename: Eng Lee
authors.initials: EL
authors.lastname: Tan
mesh_terms: D045744:Cell Line, Tumor; D029821:Enterovirus A, Human / Q000235:genetics*; D004769:Enterovirus Infections / Q000821:virology*; D006801:Humans; D007107:Immune System / Q000821:virology*; D035683:MicroRNAs; D014779:Virus Replication / Q000235:genetics*
chemical_list: D035683:MicroRNAs