title: Induction of apoptosis by 2,3-dehydrosilybin via a caspase-dependent pathway in human HeLa cells.
abstract: The aim of this study was to investigate the mechanisms involved in the apoptosis of HeLa cells due to 2,3-dehydrosilybin (DHS) treatment. DHS treatment over 24 h significantly inhibited cell viability and induced apoptosis in a dose-dependent manner. It also triggered the cleavage of caspase-8, caspase-9, caspase-3, and PARP, and significantly increased caspase-3 activity in a dose-dependent manner. Moreover, it triggered the depolarization of the mitochondrial membrane potential (Δψm), the release of cytochrome c into the cytosol, the cleavage of Bid, and the downregulation of Bcl-2 in a dose-dependent manner. Furthermore, z-VAD-fmk (a pan-caspase inhibitor) and z-IETD-fmk (a specific caspase-8 inhibitor) abolished the DHS-induced activation of the caspase-8, -9, and -3, cleavage of PARP, the depolarization of Δψm, the release of cytochrome c, the cleavage of Bid, and the downregulation of Bcl-2. Taken together, these results suggest that DHS-induced apoptosis is mediated by a caspase-dependent pathway in human HeLa cells.
authors.affiliation: a Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute , Jeongeup , Republic of Korea.
authors.forename: Byoung Ok
authors.initials: BO
authors.lastname: Cho
authors.forename: Yangkang
authors.initials: Y
authors.lastname: So
authors.forename: Chang Hyun
authors.initials: CH
authors.lastname: Jin
authors.forename: Myung Woo
authors.initials: MW
authors.lastname: Byun
authors.forename: Kwon Il
authors.initials: KI
authors.lastname: Seo
authors.forename: Kisung
authors.initials: K
authors.lastname: Ko
authors.forename: Myoung Sook
authors.initials: MS
authors.lastname: Chun
authors.forename: Il Yun
authors.initials: IY
authors.lastname: Jeong
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051023:BH3 Interacting Domain Death Agonist Protein / Q000378:metabolism; D061945:Caspase Inhibitors / Q000494:pharmacology; D020169:Caspases / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D059748:Proteolysis / Q000187:drug effects; D000077385:Silybin; D012838:Silymarin / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D051023:BH3 Interacting Domain Death Agonist Protein; D061945:Caspase Inhibitors; D012838:Silymarin; D000077385:Silybin; D045304:Cytochromes c; D020169:Caspases
keywords: 2,3-dehydrosilybin; apoptosis; caspase; cytochrome c; mitochondrial membrane potential (Δψm)