title: Localisation of the Notch family in the human endometrium of fertile and infertile women.
abstract: To investigate the spatial and temporal immunolocalisation and staining intensity of the Notch signalling family in endometrium of fertile and infertile women, endometrial biopsies were collected by curettage from 25 fertile women across the menstrual cycle and 10 infertile women in the mid secretory phase of menstrual cycle. Immunohisotchemistry was completed for NOTCH1, -2, -3, -4, cleaved Notch, DLL1, -3, -4, JAGGED1, -2, HES and NUMB and immunostaining intensity measured in both the endometrial glandular and luminal epithelium. NOTCH1 and the ligands DLL1 and JAGGED1 were key proteins displaying increased staining intensity during the receptive phase of the menstrual cycle and dysregulated in infertile endometrium. Conversely, NUMB a negative regulator of Notch signalling was decreased in the mid secretory phase of the menstrual cycle in fertile women and increased with infertility.
authors.affiliation: MIMR-PHI Institute of Medical Research, 27-31 Wright Street, Clayton, VIC, 3168, Australia.
authors.forename: Michelle
authors.initials: M
authors.lastname: Van Sinderen
authors.forename: Carly
authors.initials: C
authors.lastname: Cuman
authors.forename: Thilini
authors.initials: T
authors.lastname: Gamage
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Rainczuk
authors.forename: Tiki
authors.initials: T
authors.lastname: Osianlis
authors.forename: Luk
authors.initials: L
authors.lastname: Rombauts
authors.forename: Evdokia
authors.initials: E
authors.lastname: Dimitriadis
mesh_terms: D002135:Calcium-Binding Proteins / Q000378:metabolism; D016022:Case-Control Studies; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism* / Q000473:pathology; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D008565:Membrane Proteins / Q000378:metabolism; D021381:Protein Transport; D051880:Receptors, Notch / Q000378:metabolism*; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction
chemical_list: D002135:Calcium-Binding Proteins; C496666:DLL3 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; C490504:JAG2 protein, human; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D008565:Membrane Proteins; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins