title: Oxidative stress markers after a race in professional cyclists.
abstract: The aim was to determine the levels and activities of the oxidative stress markers in erythrocytes, plasma, and urine after a flat cyclist stage. Eight voluntary male professional trained-cyclists participated in the study. Exercise significantly increased erythrocyte, leukocyte, platelet, and reticulocyte counts. The exercise induced significant increases in the erythrocyte activities of catalase (19.8%) and glutathione reductase (19.2%), while glutathione peroxidase activity decreased significantly (29.3%). Erythrocyte GSSG concentration was significantly increased after exercise (21.4%), whereas GSH was significantly diminished (20.4%). Erythrocyte malondialdehyde levels evidenced a significant decrease 3 h after finishing the stage (44.3%). Plasma malondialdehyde, GSH and GSSG levels significantly decreased after 3 hr recovery (26.8%, 48.6%, and 31.1%, respectively). The exercise significantly increased the F2-isoprostane concentration in urine from 359 ± 71 pg/mg creatinine to 686 ± 139 pg/mg creatinine. In conclusion, a flat cycling stage induced changes in oxidative stress markers in erythrocytes, plasma, and urine of professional cyclists. Urine F2-isoprostane is a more useful biomarker for assessing the effects of acute exercise than the traditional malondialdehyde measurement.
authors.affiliation: Dept. of Biochemistry and Physiology, School of Physical Therapy, University of Valladolid, Soria, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Córdova
authors.forename: Antoni
authors.initials: A
authors.lastname: Sureda
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Albina
authors.forename: Victoria
authors.initials: V
authors.lastname: Linares
authors.forename: Montse
authors.initials: M
authors.lastname: Bellés
authors.forename: Domènec J
authors.initials: DJ
authors.lastname: Sánchez
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D056352:Athletes; D001642:Bicycling / Q000502:physiology*; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D015444:Exercise / Q000502:physiology*; D028441:F2-Isoprostanes / Q000652:urine*; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010807:Physical Endurance / Q000502:physiology*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D028441:F2-Isoprostanes; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione