title: Effects of Regioselectivity and Lipid Class Specificity of Lipases on Transesterification, Exemplified by Biodiesel Production.
abstract: Lipase-catalyzed ethanolysis of triolein was studied as a model for biodiesel production. Four lipases were immobilized on porous polypropylene, and ethanolysis reactions were carried out in methylt-butyl ether. The reaction products were analyzed using gas chromatography. Three of the four lipases studied were efficient in the conversion of triolein to 2-monoolein, but slow in the final step of producing glycerol. However,Candida antarcticalipase B was slow in the conversion of triolein, but more efficient in the subsequent two steps than the other lipases. The 1,3-selectivity of the lipases was less pronounced for the monooleins than for triolein. Silica gel was investigated as a catalyst for acyl migration, showing an increase in biodiesel yield with three of the lipases, but a reduction in yield whenC. antarcticalipase B was used. The highest biodiesel yield (96 %) was obtained with a combination ofRhizopus arrhizuslipase andC. antarcticalipase B.
authors.affiliation: Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden ; Department of Biochemistry and Molecular Biology, Vilnius University, M. K. Čiurlionio g. 21, 03101 Vilnius, Lithuania.
authors.forename: Dovilė
authors.initials: D
authors.lastname: Sinkūnienė
authors.affiliation: Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Patrick
authors.initials: P
authors.lastname: Adlercreutz
keywords: Acyl migration; Alcoholysis; Biodiesel; Lipase; Transesterification