title: Correlation of HER2 gene status assessment by fluorescence in situ hybridization between histological sections and cytological specimens of breast cancer.
abstract: BACKGROUND
  While HER2 gene detection in cytological specimens using fluorescence in situ hybridization (FISH) has been reported, the appropriate criteria for such specimens remain controversial.
  
  
  METHODS
  Fine needle aspiration (FNA) samples collected from surgically resected breast cancer specimens were rinsed in a cytopreservative solution containing fixative. Then, slides of the FNA samples were prepared by liquid-based cytology (LBC) (ThinPrep system, Hologic) according to the manufacturer's instructions, and a PathVision HER2 DNA probe kit (Abbott) was used for FISH staining. The results were evaluated using an automated MetaCyte imaging system (MetaSystems, Altlussheim, Germany). HER2 gene amplification was scored using the HER2/chromosome enumeration probe 17 (CEP17) signal count ratio as follows: amplified, >2.2; equivocal, 1.8-2.2; and unamplified, <1.8. The cytology results were compared with the histology results from concordant cases.
  
  
  RESULTS
  Successful results were obtained in 98 of 100 cases, and results from the FNA specimens were in agreement with those from the histological sections in 97 of these 98 cases (accuracy rate, 99 %; kappa, 0.962).
  
  
  CONCLUSIONS
  FISH-based assessment of the HER2 gene status is consistent between histological sections and cytological specimens of breast cancer.
authors.affiliation: Department of Clinical Laboratory, National Hospital Organization Shikoku Cancer Center, 160 Kou, Minamiumemoto-machi, Matsuyama, Ehime, 791-0280, Japan. rnishimu@shikoku-cc.go.jp.
authors.forename: Rieko
authors.initials: R
authors.lastname: Nishimura
authors.affiliation: Department of Clinical Laboratory, National Hospital Organization Shikoku Cancer Center, 160 Kou, Minamiumemoto-machi, Matsuyama, Ehime, 791-0280, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kagawa
authors.affiliation: Department of Clinical Laboratory, National Hospital Organization Shikoku Cancer Center, 160 Kou, Minamiumemoto-machi, Matsuyama, Ehime, 791-0280, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tamogami
authors.affiliation: Department of Clinical Laboratory, National Hospital Organization Shikoku Cancer Center, 160 Kou, Minamiumemoto-machi, Matsuyama, Ehime, 791-0280, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Kojima
authors.affiliation: Department of Clinical Laboratory, National Hospital Organization Shikoku Cancer Center, 160 Kou, Minamiumemoto-machi, Matsuyama, Ehime, 791-0280, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Satou
authors.affiliation: Division of Clinical Biostatistics, Section of Cancer Prevention and Epidemiology, Clinical Research Center, National Hospital Organization Shikoku Cancer Center, Matsuyama, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Yamashita
authors.affiliation: Division of Clinical Biostatistics, Section of Cancer Prevention and Epidemiology, Clinical Research Center, National Hospital Organization Shikoku Cancer Center, Matsuyama, Japan.|Department of Pathology, National Hospital Organization Shikoku Cancer Center, Matsuyama, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Teramoto
authors.affiliation: Department of Breast Oncology, National Hospital Organization Shikoku Cancer Center, Matsuyama, Japan.
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Aogi
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D044963:Biopsy, Fine-Needle; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000601:surgery; D005260:Female; D005784:Gene Amplification; D005858:Germany; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009367:Neoplasm Staging; D016612:Paraffin Embedding; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
keywords: Breast cancer; Cytology; Fluorescence in situ hybridization; HER2; Histological section