title: Low junctional adhesion molecule A expression correlates with poor prognosis in gastric cancer.
abstract: BACKGROUND
  The aberrant expression of junctional adhesion molecule A (JAM-A), which has a close correlation with the development, progression, metastasis, and prognosis of cancer, has been frequently reported. However, neither JAM-A expression nor its correlation with clinicopathologic variables and patient survival has been defined in gastric cancers. Moreover, little is known about the role of JAM-A in gastric cancer progression. We carried out the present study to investigate the prognostic value of JAM-A expression in gastric cancer patients. Furthermore, the biological roles of JAM-A in gastric cancer progression were also investigated.
  
  
  METHODS
  We determined JAM-A expression in 167 primary gastric cancer tissues and 94 matched adjacent non-tumor tissues by immunohistochemistry. Transwell migration assays and matrigel invasion assays were used to explore the role of JAM-A in gastric cancer cells migration and invasion. CCK-8 assays were used to examine the effect of JAM-A on the proliferation of gastric cancer cells.
  
  
  RESULTS
  JAM-A was downregulated in gastric cancer tissues. Low JAM-A expression was significantly associated with tumor size, lymphatic vessel invasion, lymph node metastasis, and TNM stage. Low JAM-A expression was also significantly associated with poor disease-specific survival in gastric cancer patients. Multivariate analysis demonstrated low JAM-A expression as an independent factor predicting poor survival. In addition, JAM-A had the effect on inhibition of gastric cancer cells migration and invasion. However, JAM-A had no significant effects on proliferation of gastric cancer cells.
  
  
  CONCLUSIONS
  Low JAM-A expression correlates with poor clinical outcome and promotes cell migration and invasion in gastric cancer.
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Huang
authors.affiliation: Department of Breast Surgery, First Affiliated Hospital of China Medical University, Shenyang, China. Electronic address: lovecmustar@163.com.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Xu
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Zhen-Ning
authors.initials: ZN
authors.lastname: Wang
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Yong-Xi
authors.initials: YX
authors.lastname: Song
authors.affiliation: The Research Center for Medical Genomics and MOH Key Laboratory of Cell Biology, China Medical University, Shenyang City, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Luo
authors.affiliation: The Research Center for Medical Genomics and MOH Key Laboratory of Cell Biology, China Medical University, Shenyang City, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Hui-Mian
authors.initials: HM
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D005743:Gastrectomy; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011237:Predictive Value of Tests; D011379:Prognosis; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012844:Sincalide / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality* / Q000601:surgery; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C407243:F11R protein, human; D011956:Receptors, Cell Surface; D012844:Sincalide
keywords: Gastric cancer; Invasion; JAM-A; Junctional adhesion molecule A; Migration; Prognosis