title: Helicobacter pylori infection and gastric cardia cancer in Chaoshan region.
abstract: Helicobacter pylori (H. pylori) infection represents the most important risk factor for gastric cancer, while its association with gastric cardia cancer (GCC) has not been recognized yet. In this current study, we aim to investigate the status of H. pylori infection in the gastric cardia tissue samples from high-risk populations in Chaoshan littoral region, and the relationship between H. pylori infection and chronic inflammation as well as the proliferative activity of the gastric cardia epithelial cells. A total of 706 gastric cardia biopsy specimens were obtained from 372 GCC cases and 334 tumor-free controls in Chaoshan littoral, a high-risk region for esophageal and gastric cardia cancer. Immunohistochemistry and Giemsa staining were employed for the verification of H. pylori infection. H. pylori infection rate was significantly higher in GCC (81.5%, P < 0.01) and gastric carditis (80.1%, P < 0.01) in comparison with that in the healthy group (34.8%). A significant higher prevalence of chronic inflammation was found in H. pylori+ samples (96.9%) than that in H. pylori- specimens (80.5%) (P < 0.01). To explore the possible role of H. pylori infection-related chronic inflammation in the GCC, we found that the expression of Ki-67 was progressively increased in tissues with chronic inflammation degrees from normal to severe inflammation (P < 0.01). Collectively, these results suggest that persistent H. pylori infection and the related chronic inflammation may contribute to the high incidence of GCC in Chaoshan littoral.
authors.affiliation: Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China; The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong Province, China.
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China.
authors.forename: Shuhui
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Bi
authors.affiliation: Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China.
authors.forename: Yinping
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China.
authors.forename: Runhua
authors.initials: R
authors.lastname: Lin
authors.affiliation: Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.affiliation: Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China.
authors.forename: Dongping
authors.initials: D
authors.lastname: Tian
authors.affiliation: Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China; Department of Respiratory and Critical Care Medicine of the Second Affiliated Hospital, and Department of Pharmacology, Zhejiang University School of Medicine, Hangzhou 310058, China. Electronic address: yings@zju.edu.cn.
authors.forename: Songmin
authors.initials: S
authors.lastname: Ying
authors.affiliation: Institute of Clinical Pathology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515031, Guangdong Province, China. Electronic address: minsu@stu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Su
mesh_terms: D016022:Case-Control Studies; D049109:Cell Proliferation; D002681:China / Q000453:epidemiology; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D016481:Helicobacter Infections / Q000453:epidemiology*; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D015994:Incidence; D007249:Inflammation / Q000453:epidemiology*; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000382:microbiology
keywords: Gastric cardia cancer; Helicobacter pylori; Inflammation; Ki-67