title: Kappa and lambda light chain mRNA in situ hybridization compared to flow cytometry and immunohistochemistry in B cell lymphomas.
abstract: BACKGROUND
  Detection of B cell clonality is useful for assisting in the diagnosis of B cell lymphomas. Clonality assessment can be accomplished through evaluation of KAPPA and LAMBDA light chain expression. Currently, only slide based methods are available for the majority of patient biopsies and do not detect light chain protein or mRNA in many B-cell lymphomas. Herein we evaluated a new method, known as colorimetric in situ hybridization (CISH), with improved sensitivity and multiplexing capacity, for its usefulness in clonality detection in mature B cell malignancies.
  
  
  METHODS
  The KAPPA and LAMBDA ISH was performed on a Ventana Benchmark XT utilizing two color chromogenetic detection. The probes comprised 2 haptenated riboprobes each approximately 500 base pairs long directed against the conserved regions of either KAPPA or LAMBDA mRNA. The dual colors consisted of silver deposition (black) for KAPPA light chain and a novel (pink) chromogen for LAMBDA light chain. Following optimization, CISH allowed visualization of mRNA in benign B cells in reactive tissues including germinal center, mantle zone, and post-germinal center cells. We then identified 79 cases of B cell lymphoma with formalin-fixed paraffin-embedded (FFPE) biopsies including: follicular (36 cases), mantle cell (6 cases), marginal zone (12 cases), lymphoplasmacytic (6 cases), small lymphocytic (4 cases), and diffuse large B cell (15 cases), which were selected on the basis of either prior flow cytometry or immunohistochemistry (IHC) results to serve as the predicate, "gold standard," comparator.
  
  
  RESULTS
  39/79 (49.4%) cases were classified as KAPPA and 29/79 (36.7%) as LAMBDA light chain restricted; while 9/79 (11.3%) cases were classified as indeterminate. Of the 70 cases with KAPPA or LAMBDA light chain restricted CISH, 69/70 (98.6%) were concordant with the reference method, while 1/70 (1.4%) was discordant.
  
  
  CONCLUSIONS
  Optimized CISH detected lower levels of mRNA than can be visualized with current slide based methods, making clonality assessment in FFPE biopsies possible for mature B cell neoplasms. In this preliminary study, CISH was highly accurate compared to flow cytometry or IHC. CISH offers the possibility of wider applicability of light chain ISH and is likely to become a useful diagnostic tool.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1430491067123856.
authors.affiliation: Department of Pathology, University of Arizona, Tucson, AZ, USA. lrimsza@email.arizona.edu.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Rimsza
authors.forename: William A
authors.initials: WA
authors.lastname: Day
authors.forename: Sarah
authors.initials: S
authors.lastname: McGinn
authors.forename: Anne
authors.initials: A
authors.lastname: Pedata
authors.forename: Yasodha
authors.initials: Y
authors.lastname: Natkunam
authors.forename: Roger
authors.initials: R
authors.lastname: Warnke
authors.forename: James R
authors.initials: JR
authors.lastname: Cook
authors.forename: Teresa
authors.initials: T
authors.lastname: Marafioti
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Grogan
mesh_terms: D005434:Flow Cytometry; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000032:analysis* / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000032:analysis* / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods*; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D012333:RNA, Messenger