title: Reassembly of S-layer proteins.
abstract: Crystalline bacterial cell surface layers (S-layers) represent the outermost cell envelope component in a broad range of bacteria and archaea. They are monomolecular arrays composed of a single protein or glycoprotein species and represent the simplest biological membranes developed during evolution. They are highly porous protein mesh works with unit cell sizes in the range of 3 to 30 nm, and pore sizes of 2 to 8 nm. S-layers are usually 5 to 20 nm thick (in archaea, up to 70 nm). S-layer proteins are one of the most abundant biopolymers on earth. One of their key features, and the focus of this review, is the intrinsic capability of isolated native and recombinant S-layer proteins to form self-assembled mono- or double layers in suspension, at solid supports, the air-water interface, planar lipid films, liposomes, nanocapsules, and nanoparticles. The reassembly is entropy-driven and a fascinating example of matrix assembly following a multistage, non-classical pathway in which the process of S-layer protein folding is directly linked with assembly into extended clusters. Moreover, basic research on the structure, synthesis, genetics, assembly, and function of S-layer proteins laid the foundation for their application in novel approaches in biotechnology, biomimetics, synthetic biology, and nanotechnology.
authors.affiliation: Institute of Biophysics, Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 11, 1190 Vienna, Austria.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Pum
authors.forename: Uwe B
authors.initials: UB
authors.lastname: Sleytr
mesh_terms: D001105:Archaea / Q000648:ultrastructure; D019843:Archaeal Proteins / Q000737:chemistry; D001419:Bacteria / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000737:chemistry; D032701:Biomimetics; D001709:Biotechnology; D002473:Cell Wall / Q000737:chemistry*; D008081:Liposomes; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D053769:Nanocapsules; D053758:Nanoparticles; D036103:Nanotechnology; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D019843:Archaeal Proteins; D001426:Bacterial Proteins; D008081:Liposomes; D008562:Membrane Glycoproteins; D053769:Nanocapsules; D011994:Recombinant Proteins; C055764:S-layer proteins