title: High throughput platform to explore RNA-protein interactomes.
abstract: RNA binding proteins (RBPs) and RNA interaction is an emerging topic in molecular biology. Many reports showed that such interactions contribute to many cellular processes as well as disease development. Several standard in vitro and in vivo methods were developed to fulfill the needs of this RBP-RNA interaction study to explore their biological functions. However, these methods have their limitations in terms of throughput. In this review, we emphasize two important high throughput methods to studying RBP-RNA interactions, affinity purification and protein microarray. These methods have recently become robust techniques regarding their efficiency in systematically analyzing RBP-RNA interactions. Here, we provide technique overviews, strategies and applications of these methods during biological research. Although these technologies are just beginning to be explored, they will be most important methods in this study.
authors.affiliation: a Graduate Institute of Systems Biology and Bioinformatics, National Central University , Jhongli , Taiwan.
authors.forename: F X Reymond
authors.initials: FX
authors.lastname: Sutandy
authors.affiliation: a Graduate Institute of Systems Biology and Bioinformatics, National Central University , Jhongli , Taiwan.
authors.forename: Felix Shih-Hsiang
authors.initials: FS
authors.lastname: Hsiao
authors.affiliation: a Graduate Institute of Systems Biology and Bioinformatics, National Central University , Jhongli , Taiwan.
authors.forename: Chien-Sheng
authors.initials: CS
authors.lastname: Chen
mesh_terms: D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D016601:RNA-Binding Proteins
keywords: High throughput; RNA; RNA binding protein; RNA immunoprecipitation assay; microarray; protein microarray; proteome; pull down