title: Natural co-occurrence of aflatoxins and deoxynivalenol in poultry feed in Pakistan.
abstract: Two hundred and fifteen broiler poultry feed samples were analysed over the time period of one year for the co-occurrence of aflatoxins and deoxynivalenol (DON). These were determined by GC-MS and ELISA, respectively. LOD values for aflatoxins and DON were 0.5 and 5 µg/kg, respectively. From all investigated 215 poultry feed samples, aflatoxins and DON co-occurred in 100 samples (46%). DON was detected in 114 samples while 100 samples also were positive for aflatoxins. Mean concentrations of positive samples for aflatoxins and DON were 18 and 807 µg/kg, respectively. Twenty-one DON-positive and 42 aflatoxin positive samples were contaminated above the EU maximum legal limits of 1000 µg/kg and 20 µg/kg, respectively. The present study provided useful data on aflatoxin and DON contamination, which may be helpful for future strategies to control contamination and to formulate standards in poultry feeds.
authors.affiliation: a Dr. M.A. Kazi Institute of Chemistry , University of Sindh , Jamshoro , Pakistan.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Shar
authors.forename: G A
authors.initials: GA
authors.lastname: Sumbal
authors.forename: S T H
authors.initials: ST
authors.lastname: Sherazi
authors.forename: M I
authors.initials: MI
authors.lastname: Bhanger
authors.forename: S M
authors.initials: SM
authors.lastname: Nizamani
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D057230:Limit of Detection; D010154:Pakistan; D011200:Poultry; D014255:Trichothecenes / Q000032:analysis*
chemical_list: D000348:Aflatoxins; D014255:Trichothecenes; C007262:deoxynivalenol
keywords: ELISA; GC–MS; Pakistan; aflatoxins; deoxynivalenol; poultry feed