title: Comparison of methods to isolate DNA from Caesalpinia ferrea.
abstract: Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpinia ferrea are rare, it is important to develop and/or adapt a DNA extraction protocol that produces quality DNA samples to enable the design of strategies for the conservation of this threatened species. This study aimed to compare five methods for DNA extraction and to determine the most efficient protocol for C. ferrea. Sufficient genomic DNA was obtained from the leaves of C. ferrea using all the tested protocols to perform techniques involving molecular markers. Two protocols based on the detergent cetyl trimethyl ammonium bromide, as well as a commercial kit, yielded high concentrations of pure DNA. However, when polymerase chain reaction amplifications were performed, DNA was only successfully amplified from extractions performed with the commercial kit, which produced sufficient genomic DNA of good quality from the leaves of C. ferrea to perform techniques involving molecular markers.
authors.affiliation: Departamento de Ciências Naturais, Universidade Federal do Maranhão, Campus Grajaú, Grajaú, MA, Brasil camilacampelobr@hotmail.com.
authors.forename: C C
authors.initials: CC
authors.lastname: Sousa
authors.affiliation: Departamento de Agronomia, Universidade Federal do Piauí, Campus Ininga, Teresina, PI, Brasil.
authors.forename: S O
authors.initials: SO
authors.lastname: Gomes
authors.affiliation: Departamento de Biologia, Universidade Federal do Piauí, Campus Ininga, Teresina, PI, Brasil.
authors.forename: A C A
authors.initials: AC
authors.lastname: Lopes
authors.affiliation: Departamento de Agronomia, Universidade Federal do Piauí, Campus Ininga, Teresina, PI, Brasil.
authors.forename: R L F
authors.initials: RL
authors.lastname: Gomes
authors.affiliation: Departamento de Biologia, Universidade Federal do Piauí, Campus Ininga, Teresina, PI, Brasil.
authors.forename: F B
authors.initials: FB
authors.lastname: Britto
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária, EMBRAPA Meio-Norte, Teresina, PI, Brasil.
authors.forename: P S C
authors.initials: PS
authors.lastname: Lima
authors.affiliation: Departamento de Biologia, Universidade Federal do Piauí, Campus Ininga, Teresina, PI, Brasil.
authors.forename: S E S
authors.initials: SE
authors.lastname: Valente
mesh_terms: D001965:Bromides / Q000737:chemistry; D029911:Caesalpinia / Q000235:genetics*; D018744:DNA, Plant / Q000302:isolation & purification*; D003902:Detergents / Q000737:chemistry; D018515:Plant Leaves / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D011933:Reagent Kits, Diagnostic
chemical_list: D001965:Bromides; D018744:DNA, Plant; D003902:Detergents; D000644:Quaternary Ammonium Compounds; D011933:Reagent Kits, Diagnostic; C051470:ammonium bromide