title: Role of synaptic phosphatidylinositol 3-kinase in a behavioral learning response in C. elegans.
abstract: The phosphatidylinositol 3-kinase (PI3K) pathway regulates many cellular functions, but its roles in the nervous system are still poorly understood. We found that a newly discovered insulin receptor isoform, DAF-2c, is translocated from the cell body to the synaptic region of the chemosensory neuron in Caenorhabditis elegans by a conditioning stimulus that induces taste avoidance learning. This translocation is essential for learning and is dependent on the mitogen-activated protein kinase-regulated interaction of CASY-1 (the calsyntenin ortholog) and kinesin-1. The PI3K pathway is required downstream of the receptor. Light-regulated activation of PI3K in the synaptic region, but not in other parts of the cell, switched taste-attractive behavior to taste avoidance, mimicking the effect of conditioning. Thus, synaptic PI3K is crucial for the behavioral switch caused by learning.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan. JST, CREST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Hayao
authors.initials: H
authors.lastname: Ohno
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kato
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan. Molecular Genetics Research Laboratory, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Naito
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kunitomo
authors.affiliation: Molecular Genetics Research Laboratory, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan. seidai@gen.s.u-tokyo.ac.jp iino@bs.s.u-tokyo.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tomioka
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan. JST, CREST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan. Molecular Genetics Research Laboratory, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan. seidai@gen.s.u-tokyo.ac.jp iino@bs.s.u-tokyo.ac.jp.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Iino
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D007858:Learning / Q000502:physiology*; D008027:Light; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000502:physiology*; D020033:Protein Isoforms / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism; D013569:Synapses / Q000201:enzymology*
chemical_list: C528269:CASY-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D020033:Protein Isoforms; D019869:Phosphatidylinositol 3-Kinases; C100662:AGE-1 protein, C elegans; C107483:DAF-2 protein, C elegans; D011972:Receptor, Insulin