title: The potential use of stem cells derived from human amniotic fluid in renal diseases.
abstract: Amniotic fluid (AF) contains a variety of cell types derived from fetal tissues that can easily grow in culture. These cells can be obtained during amniocentesis for prenatal screening of fetal genetic diseases, usually performed during the second trimester of pregnancy. Of particular interest, some expanded sub-populations derived from AF cells are capable of extensive self-renewal and maintain prolonged undifferentiated proliferation, which are defining properties of stem cells. These human AF stem cells (hAFSCs) exhibit multilineage potential and can differentiate into the three germ layers. They have high proliferation rates and express mesenchymal and embryonic markers, but do not induce tumor formation. In this study, hAFSCs derived from amniocentesis performed at 16-20 weeks of pregnancy were isolated, grown in culture, and characterized by flow cytometry and by their potential ability to differentiate into osteogenic, adipogenic, and chondrogenic lineages. After 4-7 passages, 5 × 105hAFSCs were inoculated under the kidney capsule of Wistar rats that were subjected to an experimental model of chronic renal disease, the 5/6 nephrectomy model (Nx). After 30 days, Nx rats treated with hAFSCs displayed significant reductions in blood pressure, proteinuria, macrophages, and α-smooth muscle actin expression compared with Nx animals. These preliminary results suggest that hAFSCs isolated and expanded from AF obtained by routine amniocentesis can promote renoprotection in the Nx model. Considering that the AF cells not used for fetal karyotyping are usually discarded, and that their use does not raise ethical issues, they may represent an alternative source of stem cells for cell therapy and regenerative medicine.
authors.affiliation: Laboratory of Cellular, Genetic, and Molecular Nephrology, Faculty of Medicine, University of São Paulo , São Paulo, Brazil.
authors.forename: Irene L
authors.initials: IL
authors.lastname: Noronha
authors.affiliation: Laboratory of Cellular, Genetic, and Molecular Nephrology, Faculty of Medicine, University of São Paulo , São Paulo, Brazil ; Laboratory of Genetic and Molecular Hematology, Faculty of Medicine, University of São Paulo , São Paulo, Brazil.
authors.forename: Rita C
authors.initials: RC
authors.lastname: Cavaglieri
authors.affiliation: Laboratory of Genetic and Molecular Hematology, Faculty of Medicine, University of São Paulo , São Paulo, Brazil.
authors.forename: Felipe L
authors.initials: FL
authors.lastname: Janz
authors.affiliation: Department of Obstetrics and Gynecology, University of São Paulo , São Paulo, Brazil.
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Duarte
authors.affiliation: Department of Obstetrics and Gynecology, University of São Paulo , São Paulo, Brazil.
authors.forename: Marco A B
authors.initials: MA
authors.lastname: Lopes
authors.affiliation: Department of Obstetrics and Gynecology, University of São Paulo , São Paulo, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Zugaib
authors.affiliation: Laboratory of Genetic and Molecular Hematology, Faculty of Medicine, University of São Paulo , São Paulo, Brazil.
authors.forename: Sergio P
authors.initials: SP
authors.lastname: Bydlowski
keywords: amniotic fluid stem cells; chronic kidney disease; mesenchymal stem cells