title: [Association between methylene-tetrahydrofolate reductase gene polymorphisms and chronic myeloid leukemia].
abstract: Methylene-tetrahydrofolate reductase (MTHFR) is a key enzyme of folate metabolism. Few studies were reported about its relationship with chronic myeloid leukemia (CML). We conducted a case-control study analyzing the prevalence of the polymorphisms MTHFR C677T and MTHFR A1298C in Algerians CML patients. Using TaqMan(®) allelic discrimination assay, we investigate MTHFR C677T and A1298C polymorphism distribution in 90 cases of CML and 100 healthy subjects. The frequencies of 677T alleles and genotypes 677TT and 677CT were significantly higher in cases than in control (P = 1E-6; OR = 6.77 [4.22-10.86]) and (P = 1E-6; OR = 10.38 [4.56-23.6]) respectively. Also, the frequencies of 1298C alleles and genotypes 1298CC and 1298AC were higher in cases (P = 9 E-6; OR = 2.65 [1.71-4.10]) and (P = 0.008; OR = 2.22 [1.21-4.06]) respectively. We report also the higher significance of the haplotype 677T/1298A and 677T/1298C in cases (P = 0.007; OR = 2.57 [1.26-5.24]) and (P = 5 E-6, OR = 6.91 [2.7646-17.2899]) respectively. Our results demonstrate that 677T and 1298C alleles are both associated with an increased risk of CML in Algeria.
authors.affiliation: Université des sciences et de la technologie d'Oran, Mohamed-Boudiaf (USTO-MB), Laboratoire de génétique moléculaire et cellulaire, BO 1505 El-Mnaouer, 31000 Oran, Algérie.
authors.forename: Samia
authors.initials: S
authors.lastname: Dorgham
authors.affiliation: Université des sciences et de la technologie d'Oran, Mohamed-Boudiaf (USTO-MB), Laboratoire de génétique moléculaire et cellulaire, BO 1505 El-Mnaouer, 31000 Oran, Algérie, Établissement hospitalier universitaire d'Oran, Service de biologie moléculaire et cytogénétique, Oran, Algérie.
authors.forename: Meriem
authors.initials: M
authors.lastname: Aberkane
authors.affiliation: Université des sciences et de la technologie d'Oran, Mohamed-Boudiaf (USTO-MB), Laboratoire de génétique moléculaire et cellulaire, BO 1505 El-Mnaouer, 31000 Oran, Algérie.
authors.forename: Wefa
authors.initials: W
authors.lastname: Boughrara
authors.affiliation: Établissement hospitalo-universitaire d'Oran, Service d'hématologie, Oran, Algérie.
authors.forename: Badra
authors.initials: B
authors.lastname: Antar Soltan
authors.affiliation: Centre hospitalo-universitaire de Mascara, Service d'hématologie, Mascara, Algérie.
authors.forename: Nemra
authors.initials: N
authors.lastname: Mehalhal
authors.affiliation: Établissement hospitalier universitaire d'Oran, Service de biologie moléculaire et cytogénétique, Oran, Algérie.
authors.forename: Hadj
authors.initials: H
authors.lastname: Touhami
authors.affiliation: Centre hospitalo-universitaire Constantine, Service d'hématologie, Constantine, Algérie.
authors.forename: Noureddine
authors.initials: N
authors.lastname: Sidimansour
authors.affiliation: Centre hospitalo-universitaire de Tlemcen, Service d'hémobiologie et de transfusions sanguines, Tlemcen, Algérie.
authors.forename: Nadia
authors.initials: N
authors.lastname: Merad Boudia
authors.affiliation: Université des sciences et de la technologie d'Oran, Mohamed-Boudiaf (USTO-MB), Laboratoire de génétique moléculaire et cellulaire, BO 1505 El-Mnaouer, 31000 Oran, Algérie.
authors.forename: Lotfi
authors.initials: L
authors.lastname: Louhibi
authors.affiliation: Université des sciences et de la technologie d'Oran, Mohamed-Boudiaf (USTO-MB), Laboratoire de génétique moléculaire et cellulaire, BO 1505 El-Mnaouer, 31000 Oran, Algérie, Établissement hospitalier universitaire d'Oran, Service de biologie moléculaire et cytogénétique, Oran, Algérie.
authors.forename: Abdallah
authors.initials: A
authors.lastname: Boudjema
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006239:Haplotypes; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: Algeria; CML; MTHFR