title: Analysis of centromere signal patterns in breast cancer cells with chromosomal instability using image cytometry combined with centromere fluorescence in situ hybridization.
abstract: Fluorescence in situ hybridization (FISH) with centromeric probes is a method used to detect chromosomal instability (CIN), a hallmark of most cancers. However, no studies thus far have investigated the relationship between centromeric FISH signals and the cell cycle in cancer cells. In this study, the chromosome content in each cell cycle phase was evaluated with respect to the number of centromeric FISH signals in two breast cancer cell lines and eight surgically resected breast cancer specimens using image cytometry. Variations in chromosome number were detected at each phase of the cell cycle but were not associated with proliferative capacity in the cell lines. Furthermore, the chromosome doubling frequency differed in each cell line and clinical specimen. These results reveal two aspects of centromeric FISH signal variation in breast cancers that exhibit CIN, and suggest that chromosome doubling is a remarkable occurrence that may increase the heterogeneity of tumors.
authors.affiliation: Department of Pathology, Yamaguchi University Graduate School of Medicine, Ube, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ito
authors.forename: Atsunori
authors.initials: A
authors.lastname: Oga
authors.forename: Kenzo
authors.initials: K
authors.lastname: Ikemoto
authors.forename: Tomoko
authors.initials: T
authors.lastname: Furuya
authors.forename: Noriko
authors.initials: N
authors.lastname: Maeda
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Shigeto
authors.initials: S
authors.lastname: Kawauchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Itoh
authors.forename: Masaaki
authors.initials: M
authors.lastname: Oka
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Sasaki
mesh_terms: D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002503:Centromere / Q000235:genetics; D043171:Chromosomal Instability / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D019044:Image Cytometry; D017404:In Situ Hybridization, Fluorescence; D061986:MCF-7 Cells; D015398:Signal Transduction / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm
keywords: aneuploidy; cell cycle; chromosomal instability; fluorescence in situ hybridization; image cytometry