title: Perilipin 3 modulates specific lipogenic pathways in SZ95 sebocytes.
abstract: Lipid droplets (LD) are dynamic organelles that manage cellular lipid synthesis, storage and retrieval. Although LD-associated proteins, including the perilipin family (PLIN1-PLIN5), are essential for these functions, they have been poorly characterized in sebocytes. Here, we employed siRNAs to downregulate PLIN3 in SZ95 sebaceous gland cells and evaluated the consequences in lipid accumulation by nile red staining and mass spectrometry. Nile red staining revealed that siRNA-mediated downregulation of PLIN3 significantly impaired linoleic acid-induced lipid accumulation in SZ95 sebocytes. Mass spectrometry revealed that PLIN3 was implicated in the metabolism of linoleic acid, a lipid source used in the build-up of triglycerides, among other acyl lipids. Furthermore, the expression of key enzymes of sebaceous lipogenesis was altered in PLIN3-deficient sebocytes, consistent with the changes observed in the neutral lipid abundance, suggesting that PLIN3 functions are intertwined with the lipogenic pathways implicated in sebaceous lipogenesis, such as desaturation and triglyceride synthesis.
authors.affiliation: Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics Research, San Gallicano Dermatologic Institute, IRCCS, Rome, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Camera
authors.forename: Maik
authors.initials: M
authors.lastname: Dahlhoff
authors.forename: Matteo
authors.initials: M
authors.lastname: Ludovici
authors.forename: Christos C
authors.initials: CC
authors.lastname: Zouboulis
authors.forename: Marlon R
authors.initials: MR
authors.lastname: Schneider
mesh_terms: D002460:Cell Line; D015536:Down-Regulation; D006801:Humans; D019787:Linoleic Acid / Q000378:metabolism; D050155:Lipogenesis / Q000235:genetics; D053858:Metabolic Networks and Pathways; D000070796:Perilipin-3; D034741:RNA, Small Interfering / Q000235:genetics; D012627:Sebaceous Glands / Q000166:cytology / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: C038196:PLIN3 protein, human; D000070796:Perilipin-3; D034741:RNA, Small Interfering; D014280:Triglycerides; D033921:Vesicular Transport Proteins; D019787:Linoleic Acid
keywords: SZ95 sebocytes; lipid droplets; perilipins; sebaceous glands