title: Epigenetic modification agents improve genomic methylation reprogramming in porcine cloned embryos.
abstract: Incomplete DNA methylation reprogramming in cloned embryos leads to low cloning efficiency. Our previous studies showed that the epigenetic modification agents 5-aza-2'-deoxycytidine (5-aza-dC) or trichostatin A (TSA) could enhance the developmental competence of porcine cloned embryos. Here, we investigated genomic methylation dynamics and specific gene expression levels during early embryonic development in pigs. In this study, our results showed that there was a typical wave of DNA demethylation and remethylation of centromeric satellite repeat (CenRep) in fertilized embryos, whereas in cloned embryos, delayed demethylation and a lack of remethylation were observed. When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts. Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos. In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence.
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
authors.forename: Yan Jun
authors.initials: YJ
authors.lastname: Huan
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.forename: Hong Mei
authors.initials: HM
authors.lastname: Wang
authors.forename: Zhan Feng
authors.initials: ZF
authors.lastname: Wu
authors.forename: Ji Guang
authors.initials: JG
authors.lastname: Zhang
authors.forename: Bing Teng
authors.initials: BT
authors.lastname: Xie
authors.forename: Jing Yu
authors.initials: JY
authors.lastname: Li
authors.forename: Qing Ran
authors.initials: QR
authors.lastname: Kong
authors.forename: Zhong Hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Hong Bin
authors.initials: HB
authors.lastname: He
mesh_terms: D000818:Animals; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D065150:Cellular Reprogramming; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D019175:DNA Methylation / Q000235:genetics*; D000077209:Decitabine; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D044127:Epigenesis, Genetic / Q000187:drug effects; D005307:Fertilization in Vitro / Q000662:veterinary; D015870:Gene Expression / Q000187:drug effects; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D013552:Swine / Q000196:embryology* / Q000235:genetics*
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A; D000077209:Decitabine; D001374:Azacitidine