title: Lipid profiling and corresponding biodiesel quality of Mortierella isabellina using different drying and extraction methods.
abstract: Four lipid extraction methods (Bligh & Dyer, hexane & isopropanol, dichloromethane & methanol, and hexane) were evaluated to extract lipid from freeze- and oven-dried fungus Mortierella isabellina ATCC42613. The highest lipid yield (41.8%) was obtained from Bligh & Dyer extraction on the oven-dried fungal biomass with a methanol:chloroform:water ratio of 2:1:0.8. Other lipid extraction methods on both freeze- and oven-dried samples had lipid yields ranging from 20.7% to 35.9%. Non-polar lipid was the main lipid class (more than 90% of total lipid) in M. isabellina. Regarding fatty acid profile, there was no significant difference on fatty acid concentration between different drying and extraction methods. Estimation of biodiesel fuel properties using correlative models further demonstrated that the fungal biodiesel is a good alternative to fossil diesel.
authors.affiliation: LABIOMAR, Institute of Biology, Federal University of Bahia, Salvador, Brazil.
authors.forename: Javid
authors.initials: J
authors.lastname: Hussain
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: LABIOMAR, Institute of Biology, Federal University of Bahia, Salvador, Brazil.
authors.forename: Iracema Andrade
authors.initials: IA
authors.lastname: Nascimento
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA. Electronic address: liaow@msu.edu.
authors.forename: Wei
authors.initials: W
authors.lastname: Liao
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D005591:Chemical Fractionation; D005227:Fatty Acids / Q000378:metabolism; D005612:Freeze Drying; D008055:Lipids / Q000096:biosynthesis* / Q000302:isolation & purification*; D020094:Mortierella / Q000378:metabolism*
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids
keywords: Biodiesel quality; Fatty acids; Fungal biomass; Lipid extraction and fractionation; Mortierella isabellina