title: Simultaneous saccharification and co-fermentation of aqueous ammonia pretreated corn stover with an engineered Saccharomyces cerevisiae SyBE005.
abstract: Co-fermentation of glucose and xylose from lignocelluloses is an efficient approach to increasing ethanol production. Simultaneous saccharification and co-fermentation (SSCF) of corn stover pretreated with aqueous ammonia was performed using engineered yeast with xylose utilization pathway. Thus far, the effect of the several key factors on SSCF was investigated, including temperature, inoculation size, pre-hydrolysis and pH. Ethanol concentration was achieved to 36.5 g/L during SSCF process with 6% glucan loading. The addition of Tween 20 reduced enzyme loading, i.e., from 15 to 7.5 FPU/gglucan with the same final ethanol concentration. The ethanol concentration was achieved to 70.1g/L at 12% glucan loading. Yeast feeding, combined with substrate and enzyme feeding, was proved to be an efficient approach for SSCF with high solid loading.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, PR China; Center of Synthetic Biology, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Jia-Qing
authors.initials: JQ
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, PR China; Center of Synthetic Biology, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, PR China; Center of Synthetic Biology, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China. Electronic address: bzli@tju.edu.cn.
authors.forename: Bing-Zhi
authors.initials: BZ
authors.lastname: Li
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, PR China; Center of Synthetic Biology, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Ying-Jin
authors.initials: YJ
authors.lastname: Yuan
mesh_terms: D000641:Ammonia / Q000494:pharmacology*; D061249:Batch Cell Culture Techniques; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D005285:Fermentation / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects; D060847:Metabolic Engineering / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014866:Waste Products; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry*
chemical_list: D014866:Waste Products; D000641:Ammonia; D014994:Xylose
keywords: Fed-batch SSCF; Fermentation with high solid content; Pre-hydrolysis; Simultaneous saccharification and co-fermentation; Surfactants