title: Production of wild-type and mutant-type human DAP12 proteins by Bombyx mori nucleopolyhedrovirus vector.
abstract: A Japanese patient with Nasu-Hakola disease was found to have a serine-to-asparagine (S39N) substitution in human DNAX-activation protein 12 (DAP12). To elucidate the functional abnormalities of mutant-type DAP12, we expressed wild-type and mutant-type recombinant DAP12 protein with Bombyx mori nucleopolyhedrovirus (BmNPV) vector, and successfully purified the respective proteins from the hemolymph of recombinant BmNPV infected B. mori larvae.
authors.affiliation: a Department of Bioinformatics and Molecular Neuropathology , Meiji Pharmaceutical University , Tokyo , Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Uemura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tabunoki
authors.forename: Taiki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Yosui
authors.initials: Y
authors.lastname: Nojima
authors.forename: Susumu
authors.initials: S
authors.lastname: Katsuma
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sato
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Satoh
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000821:virology*; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology; D015202:Protein Engineering / Q000379:methods*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008565:Membrane Proteins; D050505:Mutant Proteins; C110546:TYROBP protein, human
keywords: Bombyx mori nucleopolyhedrovirus (BmNPV) vector; DNAX-activation protein 12 (DAP12); HBM signal-fused DAP12 protein; Nasu-Hakola disease (NHD); silkworm