title: Identification and evaluation of anti-inflammatory compounds from Kaempferia parviflora.
abstract: The rhizome of Kaempferia parviflora has been used in traditional Thai medicine. In this study, we identified and compared specific compounds from the hexane extract of K. parviflora with those from other Zingiberaceous plants by using gas chromatography-mass spectrometry. We identified 5,7-dimethoxyflavone (DMF), 5-hydroxy-3,7,3',4'-tetramethoxyflavone (TMF), estimated 3,5,7-trimethoxyflavone, 5-hydroxy-7,4'-dimethoxyflavone, 3,5,7,4'-tetramethoxyflavone, and investigated their anti-inflammatory effects in rat basophilic leukemia (RBL-2H3) cells stimulated with an IgE antigen or a calcium ionophore. We found that DMF and TMF more potently inhibited antigen-induced degranulation than did nobiletin, a well-known anti-inflammatory agent. In addition, compared to RBL-2H3 cells stimulated with a calcium ionophore, those treated with DMF and TMF showed more marked inhibition of the degranulation and the production and mRNA expression of inflammatory mediators. These results suggest that DMF and TMF inhibit an early step in the high-affinity IgE receptor signaling cascade rather than intracellular calcium release and protein kinase C activation.
authors.affiliation: a Laboratory of Nutrition , Graduate School of Agricultural Science, Tohoku University , Sendai , Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Horigome
authors.forename: Izumi
authors.initials: I
authors.lastname: Yoshida
authors.forename: Aiko
authors.initials: A
authors.lastname: Tsuda
authors.forename: Teppei
authors.initials: T
authors.lastname: Harada
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Shuichi
authors.initials: S
authors.lastname: Inohana
authors.forename: Satoshi
authors.initials: S
authors.lastname: Isagawa
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kibune
authors.forename: Toshiya
authors.initials: T
authors.lastname: Satoyama
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Katsuda
authors.forename: Shinobu
authors.initials: S
authors.lastname: Suzuki
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Watai
authors.forename: Naoto
authors.initials: N
authors.lastname: Hirose
authors.forename: Takahiro
authors.initials: T
authors.lastname: Mitsue
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shirakawa
authors.forename: Michio
authors.initials: M
authors.lastname: Komai
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006586:Hexanes / Q000737:chemistry; D018836:Inflammation Mediators / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D006586:Hexanes; D018836:Inflammation Mediators; D010936:Plant Extracts
keywords: GC–MS analysis; Kaempferia parviflora; anti-inflammation; inflammatory mediator; polymethoxyflavonoid