title: In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity.
abstract: Single-nucleotide polymorphisms, either inherited or due to spontaneous DNA damage, are associated with numerous diseases. Developing tools for site-specific nucleotide modification may one day provide a way to alter disease polymorphisms. Here, we describe the in vitro selection and characterization of a new deoxyribozyme called F-8, which catalyzes nucleotide excision specifically at thymidine. Cleavage by F-8 generates 3'- and 5'-phosphate ends recognized by DNA modifying enzymes, which repair the targeted deoxyribonucleotide while maintaining the integrity of the rest of the sequence. These results illustrate the potential of DNAzymes as tools for DNA manipulation.
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, P.R. China Department of Chemistry, Key Laboratory of Green Chemistry and Technology (Ministry of Education), Sichuan University, Chengdu 610064, P.R. China.
authors.forename: Mingqi
authors.initials: M
authors.lastname: Wang
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, P.R. China.
authors.forename: Huafan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, P.R. China.
authors.forename: Yongyun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, P.R. China.
authors.forename: Afshan
authors.initials: A
authors.lastname: Yasmeen
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Key Laboratory of Green Chemistry and Technology (Ministry of Education), Sichuan University, Chengdu 610064, P.R. China tangzhuo@cib.ac.cn.
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Yu
authors.affiliation: Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041, P.R. China tangzhuo@cib.ac.cn.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Tang
mesh_terms: D053837:DNA Cleavage; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism*
chemical_list: D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D013936:Thymidine