title: In vitro motility changes in Roux limb after Roux-en-Y reconstruction.
abstract: BACKGROUND
  The aim of this study is to compare the motility of Roux limb with that of normal segment.
  
  
  METHODS
  Rats were divided into 3 equal groups as control, Roux-en-Y (Group A) and Roux-en-Y with vagotomy (Group B). Only midline incision and manipulations were applied in control group. Following distal gastrectomy, Roux-en-Y reconstruction was applied in Group A, while vagotomy Roux-en-Y gastrojejunostomy was applied in Group B. Rats were sacrificed 1 month later by cervical dislocations under anesthesia. The obtained jejunal segments were cut into four equal parts. The bath was 37 °C warm while 95 % O2 and 5 % CO2 gases were supplied in 10 ml bicarbonate Krebs' solution.
  
  
  RESULTS
  KCl responses were similar in all three groups. Acetylcholine contraction responses in the vagotomy and non-vagotomy Roux-en-Y groups was higher than in those in control group significantly (p < 0.05). This response in vagotomy Roux-en-Y group was also higher than that in non-vagotomy group (p < 0.05). The induced electrical field stimulation contraction response in the vagotomy + Roux-en-Y group was lower than those in control group and non-vagotomy group (p < 0.05).
  
  
  CONCLUSIONS
  These results show that muscarinic receptor density and/or function may increase after vagotomy and non-vagotomy group operation, and vagotomy may contribute to this increase. The decrease in electrical signal response in vagotomy Roux-en-Y group may depend on the decrease in acetylcholine oscillation from the cholinergic nerve ending (Tab. 1, Fig. 5, Ref. 25).
authors.forename: N
authors.initials: N
authors.lastname: Dogan
authors.forename: A K
authors.initials: AK
authors.lastname: Gokakin
authors.forename: A
authors.initials: A
authors.lastname: Koyuncu
authors.forename: I
authors.initials: I
authors.lastname: Bagcivan
authors.forename: C
authors.initials: C
authors.lastname: Aydin
authors.forename: O
authors.initials: O
authors.lastname: Topcu
authors.forename: S
authors.initials: S
authors.lastname: Yildirim
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000713:Anastomosis, Roux-en-Y; D000818:Animals; D018679:Cholinergic Agonists / Q000494:pharmacology; D004558:Electric Stimulation; D005743:Gastrectomy; D018589:Gastroparesis / Q000209:etiology* / Q000503:physiopathology; D007583:Jejunum / Q000503:physiopathology*; D008297:Male; D009130:Muscle, Smooth / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D046509:Tissue Culture Techniques; D014628:Vagotomy
chemical_list: D018679:Cholinergic Agonists; D000109:Acetylcholine