title: Improvement of the transformation efficiency of Sacchaaromyces cerevisiae by altering carbon sources in pre-culture.
abstract: We show here that the transformation efficiency of Saccharomyces cerevisiae is improved by altering carbon sources in media for pre-culturing cells prior to the transformation reactions. The transformation efficiency was increased up to sixfold by combination with existing transformation protocols. This method is widely applicable for yeast research since efficient transformation can be performed easily without changing any of the other procedures in the transformation.
authors.affiliation: a Laboratory of Molecular Biology, Graduate School of Agricultural Science , Tohoku University , Sendai , Japan.
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Konishi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Harata
mesh_terms: D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000032:analysis / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic / Q000187:drug effects*
chemical_list: D003470:Culture Media; D005947:Glucose
keywords: Saccharomyces cerevisiae; high-efficient transformation; low-glucose medium