title: Semisynthesis of a post-translationally modified protein by using chemical cleavage and activation of an expressed fusion polypeptide.
abstract: Herein, we describe a new semisynthetic strategy of a post-translationally modified protein in which the middle region is glycosylated. We designed a single-plasmid coding for a fusion polypeptide, which can provide both an N-terminal α-thioester and a C-terminal cysteine peptide of a target glycoprotein by using chemical-cleavage and activation methods. The use of these resultant peptide derivatives resulted in the successful synthesis of N-glycosylated-interleukin 13.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, Machikaneyama, Toyonaka, Osaka 5600043 (Japan), Fax: (+81) 668505382.
authors.forename: Ryo
authors.initials: R
authors.lastname: Okamoto
authors.forename: Motoharu
authors.initials: M
authors.lastname: Kimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ishimizu
authors.forename: Masayuki
authors.initials: M
authors.lastname: Izumi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kajihara
mesh_terms: D003545:Cysteine / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006031:Glycosylation; D018793:Interleukin-13 / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D018793:Interleukin-13; D010455:Peptides; D011993:Recombinant Fusion Proteins; D003545:Cysteine
keywords: cleavage reaction; glycopeptide; native chemical ligation; protein; semisynthesis