title: Isolation and characterization of a cryptogein-like gene from drought and salt-treated Alternanthera philoxeroides roots.
abstract: Alligator weed, Alternanthera philoxeroides (Mart.) Grisb, is an amphibious plant with long thick fleshy roots that develop from adventitious roots under drought conditions. To clone differentially-expressed genes from the roots of A. philoxeroides we applied both mRNA differential display and rapid amplification of cDNA ends techniques. A cryptogein-like gene of A. philoxeroides, designated as ApCL, was cloned. On the basis of semi-quantitative RT-PCR analysis results, we demonstrated that the ApCL gene was upregulated under drought and salt stress conditions. After ApCL was transferred to Pichia pastoris GS115 and its resistance to drought and salt then increased by >100 %. Therefore, the ApCL gene of A. philoxeroides was likely involved in drought and salt tolerance responses.
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, P.R China/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Gao
authors.forename: Jinzhi
authors.initials: J
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xiao
authors.forename: Caibo
authors.initials: C
authors.lastname: Luo
authors.forename: Shiqing
authors.initials: S
authors.lastname: Zhang
authors.forename: Helong
authors.initials: H
authors.lastname: Chen
authors.forename: Kexian
authors.initials: K
authors.lastname: Yi
mesh_terms: D027703:Amaranthaceae / Q000235:genetics* / Q000502:physiology*; D000595:Amino Acid Sequence; D055864:Droughts; D005656:Fungal Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055049:Salt Tolerance / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; C061032:cryptogein protein, Phytophthora cryptogea