title: Evaluation of the effect of expansion and shear stress on a self-assembled endothelium mimicking nanomatrix coating for drug eluting stents in vitro and in vivo.
abstract: Coating stability is increasingly recognized as a concern impacting the long-term effectiveness of drug eluting stents (DES). In particular, unstable coatings have been brought into focus by a recently published report (Denardo et al 2012 J. Am. Med. Assoc. 307 2148-50). Towards the goal of overcoming current challenges of DES performance, we have developed an endothelium mimicking nanomatrix coating composed of peptide amphiphiles that promote endothelialization, but limit smooth muscle cell proliferation and platelet adhesion. Here, we report a novel water evaporation based method to uniformly coat the endothelium mimicking nanomatrix onto stents using a rotational coating technique, thereby eliminating residual chemicals and organic solvents, and allowing easy application to even bioabsorbable stents. Furthermore, the stability of the endothelium mimicking nanomatrix was analyzed after force experienced during expansion and shear stress under simulated physiological conditions. Results demonstrate uniformity and structural integrity of the nanomatrix coating. Preliminary animal studies in a rabbit model showed no flaking or peeling, and limited neointimal formation or restenosis. Therefore, it has the potential to improve the clinical performance of DES by providing multifunctional endothelium mimicking characteristics with structural integrity on stent surfaces.
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, Alabama, USA.
authors.forename: Adinarayana
authors.initials: A
authors.lastname: Andukuri
authors.forename: IlJae
authors.initials: I
authors.lastname: Min
authors.forename: Patrick
authors.initials: P
authors.lastname: Hwang
authors.forename: Grant
authors.initials: G
authors.lastname: Alexander
authors.forename: Lauren E
authors.initials: LE
authors.lastname: Marshall
authors.forename: Joel L
authors.initials: JL
authors.lastname: Berry
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Wick
authors.forename: Yoon Ki
authors.initials: YK
authors.lastname: Joung
authors.forename: Young-Sup
authors.initials: YS
authors.lastname: Yoon
authors.forename: Brigitta C
authors.initials: BC
authors.lastname: Brott
authors.forename: Dong Keun
authors.initials: DK
authors.lastname: Han
authors.forename: Ho-Wook
authors.initials: HW
authors.lastname: Jun
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods*; D054855:Drug-Eluting Stents / Q000592:standards*; D042783:Endothelial Cells / Q000166:cytology*; D004727:Endothelium / Q000166:cytology; D006801:Humans; D007083:Iliac Artery / Q000601:surgery; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D033081:Shear Strength
chemical_list: D020099:Coated Materials, Biocompatible