title: Development of a sensitive LC-MS/MS method for simultaneous quantification of eleven constituents in rat serum and its application to a pharmacokinetic study of a Chinese medicine Shengmai injection.
abstract: A sensitive LC-MS/MS method was developed and validated for simultaneous quantification of 11 constituents, ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rd, Rc, ophiopogonin D, schisandrin, schisandrol B and schizandrin B, in rat serum using digoxin as the internal standard (IS). The serum samples were pretreated and extracted with a two-step liquid-liquid extraction. Chromatographic separation was achieved on a C18 analytical column with a proper gradient elution using 0.02% acetic acid aqueous solution and 0.02% acetic acid-acetonitrile as mobile phase at a flow rate of 0.5 mL/min. MS detection was performed using multiple reaction monitoring via an electrospray ionization source. Good linearity was observed in the validated concentration range for every analyte (r(2) ≥0.9929), and the lower limits of quantitation of the analytes were in the range of 0.044-1.190 ng/mL in rat serum. Intra- and inter-day precisions were <14.2%. The accuracy expressed as recovery was within the range of 85.1-112.8%. The extraction recoveries were >75.8%.The validated method was successfully applied to a pharmacokinetic study of all analytes in rats after single intravenous administration of Shengmai injection.
authors.affiliation: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China; Department of Pharmaceutics, Medical College of Jiaxing University, Jiaxing, 314001, China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhan
authors.forename: Qing
authors.initials: Q
authors.lastname: Shao
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Fan
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D036145:Ginsenosides / Q000097:blood*; D017705:Lignans / Q000097:blood*; D051381:Rats; D012503:Saponins / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D036145:Ginsenosides; D017705:Lignans; D012503:Saponins
keywords: LC-MS/MS; Shengmai injection; ginsenoside; lignan; ophiopogonin; pharmacokinetics