title: The impact of exogenous DNA on the structure of sperm of olive flounder (Paralichthys olivaceus).
abstract: Sperm-mediated gene transfer (SMGT) is a promising transgenic technology that relies on the capability of sperm to internalize exogenous DNA. In marine fish, however, the interaction between sperm and exogenous DNA appears to be deficient. Here, we demonstrated significant DNase activity in the seminal plasma of the olive flounder. When incubated with naked-DNA, the spermatozoa lost their structural integrity, including the head, mitochondria and flagellum, in an incubation time-dependent manner. However, internalization of a liposome-DNA complex resulted in the structural integrity of the spermatozoa being maintained, even when using incubation times of up to 50min. We concluded that in the olive flounder, SMGT is possible by integrating liposome-DNA complexes, rather than naked-DNA alone, into the sperm. In brief, removal of the seminal plasma and packaging the exogenous DNA were necessary for successful SMGT in the olive flounder.
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, Ocean University of China, Qingdao, Shandong, 266003, China.
authors.forename: Nian
authors.initials: N
authors.lastname: Xin
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, Ocean University of China, Qingdao, Shandong, 266003, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, Ocean University of China, Qingdao, Shandong, 266003, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, Ocean University of China, Qingdao, Shandong, 266003, China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, Ocean University of China, Qingdao, Shandong, 266003, China.
authors.forename: Jinxiang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, Ocean University of China, Qingdao, Shandong, 266003, China.
authors.forename: Quanqi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding, College of Marine Life Science, Ocean University of China, Qingdao, Shandong, 266003, China. Electronic address: qijie@ouc.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Qi
mesh_terms: D000818:Animals; D003851:Deoxyribonucleases / Q000378:metabolism; D005432:Flounder / Q000502:physiology*; D008081:Liposomes; D008297:Male; D012661:Semen / Q000201:enzymology; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology; D019076:Transgenes / Q000502:physiology*
chemical_list: D008081:Liposomes; D003851:Deoxyribonucleases
keywords: DNase; Liposome-DNA complexes; Naked-DNA; Paralichthys olivaceus; Sperm-mediated gene transfer (SMGT)