title: Selective retention of basic compounds by metal aquo-ion affinity chromatography.
abstract: A novel metal aquo-ion affinity chromatography has been developed for the analysis of basic compounds using heat-treated silica gel containing hydrated metal cations (metal aquo-ions) as the packing material. The packing materials of the metal aquo-ion affinity chromatography were prepared by the immobilization of a single metal component such as Fe(III), Al(III), Ag(I), and Ni(II) on silica gel followed by extensive heat treatment. The immobilized metals form aquo-ions to present cation-exchange ability for basic analytes and the cation-exchange ability for basic analytes depends on pKa of the immobilized metal species. In the present study, to evaluate the retention characteristics of metal aquo-ion affinity chromatography, the on-line solid-phase extraction of drugs was investigated. Obtained data clearly evidence the selective retention capability of metal aquo-ion affinity chromatography for basic analytes with sufficient capacity.
authors.affiliation: Analysis Group, Tsukuba Division, Sunplanet Co., Ltd., Tokodai, Tsukuba, Ibaraki, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Asakawa
authors.forename: Eiichi
authors.initials: E
authors.lastname: Yamamoto
authors.forename: Naoki
authors.initials: N
authors.lastname: Asakawa
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry; D002411:Cation Exchange Resins / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver / Q000737:chemistry
chemical_list: D017607:Aluminum Compounds; D002411:Cation Exchange Resins; D005290:Ferric Compounds; D005782:Gels; D007477:Ions; D008670:Metals; D012834:Silver; D012822:Silicon Dioxide; D009532:Nickel
keywords: Basic compounds; Heat-treated silica gel; High-performance liquid chromatography; Metal aquo-ions