title: Chemical structure and biological activity of a highly branched (1 → 3,1 → 6)-β-D-glucan from Isochrysis galbana.
abstract: A highly branched (1 → 3,1 → 6)-β-D-glucan was isolated from the microalga Isochrysis galbana Parke (Isochrysidales, Haptophyta). The polysaccharide structure was analyzed by methylation and Smith degradation, as well as by ESI and MALDI TOF mass spectrometry and NMR spectroscopy. The glucan was shown to contain a (1 → 6)-linked backbone, where every residue is substituted at position 3 by Glc, which in turn may be substituted at C-6 by a single Glc or by rather short (up to tetrasaccharide) oligosaccharide chains. All the 3-linked Glc residues are present in these side chains. In the biological activity experiments it was demonstrated that the polysaccharide directly inhibits the proliferation of U937 human leukemic monocyte lymphoma cells and therefore has potential anti-tumor activity.
authors.affiliation: Equipe BPA, USC ANSES, Université du Littoral-Côte d'Opale, Boulogne sur Mer, France.
authors.forename: Irina
authors.initials: I
authors.lastname: Sadovskaya
authors.affiliation: Laboratoire de Océanologie et de Géosciences, UMR CNRS 8187 LOG, Université de Lille 1, Wimereux, France.
authors.forename: Anissa
authors.initials: A
authors.lastname: Souissi
authors.affiliation: Laboratoire de Océanologie et de Géosciences, UMR CNRS 8187 LOG, Université de Lille 1, Wimereux, France.
authors.forename: Sami
authors.initials: S
authors.lastname: Souissi
authors.affiliation: Equipe BPA, USC ANSES, Université du Littoral-Côte d'Opale, Boulogne sur Mer, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Grard
authors.affiliation: Equipe BPA, USC ANSES, Université du Littoral-Côte d'Opale, Boulogne sur Mer, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lencel
authors.affiliation: Department Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Greene
authors.affiliation: Department Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Sarah
authors.initials: S
authors.lastname: Duin
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East Branch of Russian Academy of Sciences, Vladivostok, Russian Federation.
authors.forename: Pavel S
authors.initials: PS
authors.lastname: Dmitrenok
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii prosp. 47, Moscow, Russian Federation.
authors.forename: Alexander O
authors.initials: AO
authors.lastname: Chizhov
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii prosp. 47, Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii prosp. 47, Moscow, Russian Federation. Electronic address: usov@ioc.ac.ru.
authors.forename: Anatolii I
authors.initials: AI
authors.lastname: Usov
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D058087:Haptophyta / Q000737:chemistry*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000188:drug therapy / Q000473:pathology; D008969:Molecular Sequence Data; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D047071:beta-Glucans
keywords: (1→3,1→6)-β-d-Glucan; Antiproliferative activity; Isochrysis galbana; Microalgae