title: 3D origami electrochemical immunodevice for sensitive point-of-care testing based on dual-signal amplification strategy.
abstract: A dual signal amplification immunosensing strategy that offers high sensitivity and specificity for the detection of low-abundance biomarkers was designed on a 3D origami electrochemical device. High sensitivity was achieved by using novel Au nanorods modified paper working electrode (AuNRs-PWE) as sensor platform and metal ion-coated Au/bovine serum albumin (Au/BSA) nanospheres as tracing tags. High specificity was further obtained by the simultaneous measurement of two cancer markers on AuNRs-PWE surface using different metal ion-coated Au/BSA tracers. The metal ions could be detected directly through differential pulse voltammetry (DPV) without metal preconcentration, and the distinct voltammetric peaks had a close relationship with each sandwich-type immunoreaction. The position and size of the peaks reflected the identity and level of the corresponding antigen. Integrating the dual-signal amplification strategy, a novel 3D origami electrochemical immunodevice for simultaneous detecting carcinoembryonic antigen (CEA) and cancer antigen 125 (CA125) with linear ranges of over 4 orders of magnitude with detection limits down to 0.08 pg mL(-1) and 0.06 mU mL(-1) was successfully developed. This strategy exhibits high sensitivity and specificity with excellent performance in real human serum assay. The AuNRs-PWE and the designed tracer on this immunodevice provided a new platform for low-cost, high-throughput and multiplex immunoassay and point-of-care testing in remote regions, developing or developed countries.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Qingkun
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Zhaoquan
authors.initials: Z
authors.lastname: Bian
authors.affiliation: Cancer Research Center, Shandong Tumor Hospital, Jinan 250117, PR China.
authors.forename: Xianrang
authors.initials: X
authors.lastname: Song
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: ujn.yujh@gmail.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation*; D018394:CA-125 Antigen / Q000235:genetics / Q000302:isolation & purification*; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000302:isolation & purification*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification*; D053768:Metal Nanoparticles / Q000737:chemistry; D043942:Nanotubes / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics; D012709:Serum Albumin / Q000737:chemistry / Q000276:immunology
chemical_list: D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D002272:Carcinoembryonic Antigen; C451759:MUC16 protein, human; D008565:Membrane Proteins; D012709:Serum Albumin; D006046:Gold
keywords: 3D origami device; Electrochemical; Multiplex immunoassay; Point-of-care testing