title: Cloning and expression analysis of G-protein Gαq subunit and Gβ1 subunit from Bemisia tabaci Gennadius (Homoptera: Aleyrodidae).
abstract: The heterotrimeric G proteins play an essential role in a wide variety of signal transduction pathways, mediating the process of chemical signals from the environment in all higher eukaryotic organisms. In this article, two G-protein subunit genes encoding Gαq and Gβ1 were cloned from Bemisia tabaci Gennadius. The full-length cDNA sequence of BtGαq consisted of 2,336 bp with an ORF of 1,062 bp encoding 353 amino acids and BtGβ1 had a full length of 1,942 bp with an ORF of 1,023 nucleotides encoding 340 amino acids. The amino acid sequences of BtGαq and BtGβ1 from B. tabaci B biotype were identical to those from the Q biotype. Phylogenetic analysis identified G protein α and β subunit families from insects based on their amino acid sequences. The expression patterns of BtGαq and BtGβ1 at different development stages and in different body regions were analyzed by real-time quantitative PCR and Western blot. The results show that BtGαq and BtGβ1 are neither developmental stage-specific nor tissue-specific. The transcript levels of BtGαq in the B biotype are similar to that in the Q biotype, the transcript levels of BtGβ1 at egg, first instar and pupae in B biotype were significantly higher than that in Q biotype. The transcript levels of BtGαq and BtGβ1 in the head were significantly higher than those in thorax and abdomen indicating that they are involved in nervous system and sensory functions.
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, China; Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Tu
authors.forename: Yuchuan
authors.initials: Y
authors.lastname: Qin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D005260:Female; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000096:biosynthesis / Q000235:genetics*; D044387:GTP-Binding Protein beta Subunits / Q000096:biosynthesis / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006430:Hemiptera / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D044387:GTP-Binding Protein beta Subunits; D012313:RNA; D043802:GTP-Binding Protein alpha Subunits, Gq-G11
keywords: Bemisia tabaci; Gαq subunit; Gβ1 subunit; expression pattern; gene cloning