title: Expression patterns of SDF1/CXCR4 in human invasive breast carcinoma and adjacent normal stroma: correlation with tumor clinicopathological parameters and patient survival.
abstract: SDF-1/CXCR4 axis is involved in various steps of breast tumorigenesis such as tumor growth, angiogenesis and metastasis. The goal of the present study is to demonstrate in detail the immunohistochemical distribution of SDF-1 and CXCR4 in invasive breast carcinomas and identify possible correlation of their expression patterns with clinicopathological parameters and patients survival. We investigated the immunoexpression of CXCR4 and SDF1 in 76 invasive breast carcinomas. Both SDF-1 and CXCR4 had statistically significant higher expression in carcinomas compared with adjacent normal breast tissue. Furthermore the expression of CXCR4 in intratumoral fibroblasts had a positive correlation with overall and disease-free survival, while SDF1 membranous immunopositivity in normal breast epithelial cells was a risk factor for relapse. In addition, expression of SDF1 in fibroblasts of normal breast tissue was positively associated with tumor grade. Overall, our results suggest that the differential expression of CXCR4 in intratumoral stroma and SDF1 in adjacent normal mammary cells may predict clinical outcome in breast cancer patients.
authors.affiliation: Department of Anatomy, School of Medicine, University of Patras, Patras, Greece. Electronic address: bpbp@upatras.gr.
authors.forename: Haralabos
authors.initials: H
authors.lastname: Papatheodorou
authors.affiliation: Clinical and Molecular Oncology Laboratory, Division of Oncology, School of Medicine, University of Patras, Patras, Greece; Department of Pathology, Patras General Hospital, Patras, Greece.
authors.forename: Anastasios D
authors.initials: AD
authors.lastname: Papanastasiou
authors.affiliation: Clinical and Molecular Oncology Laboratory, Division of Oncology, School of Medicine, University of Patras, Patras, Greece.
authors.forename: Chaido
authors.initials: C
authors.lastname: Sirinian
authors.affiliation: Department of Pathology, School of Medicine, University of Patras, Patras, Greece.
authors.forename: Chrisoula
authors.initials: C
authors.lastname: Scopa
authors.affiliation: Clinical and Molecular Oncology Laboratory, Division of Oncology, School of Medicine, University of Patras, Patras, Greece.
authors.forename: Haralabos P
authors.initials: HP
authors.lastname: Kalofonos
authors.affiliation: Laboratory of Public Health, Medical School, University of Patras, Patras, Greece.
authors.forename: Michalis
authors.initials: M
authors.lastname: Leotsinidis
authors.affiliation: Department of Anatomy, School of Medicine, University of Patras, Patras, Greece.
authors.forename: Helen
authors.initials: H
authors.lastname: Papadaki
mesh_terms: D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D045744:Cell Line, Tumor; D054377:Chemokine CXCL12 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local / Q000378:metabolism / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4
keywords: Breast cancer; CXCR4; Immunohistochemistry; SDF1