title: [The up-regulation of p-p38 MAPK during the induction of brain ischemic tolerance induced by intermittent hypobaric hypoxia preconditioning in rats].
abstract: OBJECTIVE
  To explore the expression of p-p38 MAPK protein and the number of astrocytes expressing p-p38 MAPK in CA1 hippocampus in rats during the induction of brain ischemic tolerance induced by intermittent hypobaric hypoxia (IH) preconditioning.
  
  
  METHODS
  Thirty healthy adult male Wistar rats were randomly divided into 6 groups (n = 5 in each group): sham 0 min group, IH + sham 0 min group, sham 7 d group, IH + sham 7 d group, Ischemia (Is) 7 d group, and IH + Is 7 d group. Neuropathological evaluation was performed by thionine staining in CA1 hippocampus in rats. The expression of p-p38 MAPK in CA1 hippocampus was observed by immunohistochemical staining. And the number of astrocytes expressing p-p38 MAPK was observed by immunofluorescent double labeling.
  
  
  RESULTS
  The results showed that IH preconditioning induced brain ischemic tolerance successfully. At the same time, IH preconditioning obviously up-regulated the expression of p-p38 MAPK protein in CA1 hippocampus, and also increased the number of astrocytes expressing p-p38 MAPK.
  
  
  CONCLUSIONS
  It might be concluded that IH preconditioning induced brain ischemic tolerance by up-regulating the expression of p-p38 MAPK protein in pyramidal neurones and astrocytes.
authors.forename: Xin-Ying
authors.initials: XY
authors.lastname: Bi
authors.forename: Tian-Shuang
authors.initials: TS
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Liu
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000201:enzymology / Q000473:pathology; D002545:Brain Ischemia / Q000201:enzymology* / Q000473:pathology; D004195:Disease Models, Animal; D006624:Hippocampus / Q000201:enzymology; D000860:Hypoxia; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D010766:Phosphorylation; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D048051:p38 Mitogen-Activated Protein Kinases