title: Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE.
abstract: The antioxidant and antibacterial properties of Greek oregano extracts obtained by fractional supercritical fluid extraction (SFE) with carbon dioxide were investigated and compared with the properties of essential oil obtained by hydrodistillation. According to DPPH, hydroxyl radical and superoxide anion radical scavenging activity assays, the supercritical extracts expressed stronger antioxidant activity comparing to the essential oil. The most effective was the supercritical extract obtained by fractional extraction at 30 MPa and 100°C after the volatile fraction had been extracted at lower pressure. At the same time this extract showed strong antibacterial activity against staphylococci, including MRSA strain, but did not affect Escherichia coli of normal intestinal flora. The essential oil obtained by hydrodistillation showed stronger antibacterial activity against E. coli, Salmonella and Klebsiella pneumoniae, comparing to the supercritical extracts but at the same affected the normal gut flora.
authors.affiliation: University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia. Electronic address: stamena@tmf.bg.ac.rs.
authors.forename: Marko
authors.initials: M
authors.lastname: Stamenic
authors.affiliation: University of Novi Sad, Faculty of Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Vulic
authors.affiliation: University of Novi Sad, Faculty of Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
authors.forename: Sonja
authors.initials: S
authors.lastname: Djilas
authors.affiliation: University of Belgrade, Faculty of Veterinary Medicine, Bulevar oslobodjenja 18, 11000 Belgrade, Serbia.
authors.forename: Dusan
authors.initials: D
authors.lastname: Misic
authors.affiliation: Institute for Medicinal Plant Research "Dr. Josif Pancic", Tadeusa Koscuska 1, 11000 Belgrade, Serbia.
authors.forename: Vanja
authors.initials: V
authors.lastname: Tadic
authors.affiliation: University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia.
authors.forename: Slobodan
authors.initials: S
authors.lastname: Petrovic
authors.affiliation: University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, Serbia.
authors.forename: Irena
authors.initials: I
authors.lastname: Zizovic
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D016166:Free Radical Scavengers / Q000737:chemistry*; D031341:Origanum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts
keywords: Antibacterial activity; Greek oregano; Intestinal flora; MRSA; Natural antioxidants; Supercritical extraction