title: Imaging cleared intact biological systems at a cellular level by 3DISCO.
abstract: Tissue clearing and subsequent imaging of transparent organs is a powerful method to analyze fluorescently labeled cells and molecules in 3D, in intact organs. Unlike traditional histological methods, where the tissue of interest is sectioned for fluorescent imaging, 3D imaging of cleared tissue allows examination of labeled cells and molecules in the entire specimen. To this end, optically opaque tissues should be rendered transparent by matching the refractory indices throughout the tissue. Subsequently, the tissue can be imaged at once using laser-scanning microscopes to obtain a complete high-resolution 3D image of the specimen. A growing list of tissue clearing protocols including 3DISCO, CLARITY, Sca/e, ClearT2, and SeeDB provide new ways for researchers to image their tissue of interest as a whole. Among them, 3DISCO is a highly reproducible and straightforward method, which can clear different types of tissues and can be utilized with various microscopy techniques. This protocol describes this straightforward procedure and presents its various applications. It also discusses the limitations and possible difficulties and how to overcome them.
authors.affiliation: Neuroscience, Genentech, Inc.; ali.erturk.neuro@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Ertürk
authors.affiliation: Department of Discovery Oncology, Genentech, Inc.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lafkas
authors.affiliation: Department of Pathology, Genentech, Inc.
authors.forename: Cecile
authors.initials: C
authors.lastname: Chalouni
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D005260:Female; D005663:Furans / Q000737:chemistry; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008168:Lung / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal / Q000379:methods; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D061848:Optical Imaging / Q000379:methods*; D010647:Phenyl Ethers / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013048:Specimen Handling / Q000379:methods; D013116:Spinal Cord / Q000737:chemistry / Q000166:cytology
chemical_list: D005663:Furans; D010647:Phenyl Ethers; D012997:Solvents; C076624:dibenzyl ether; C018674:tetrahydrofuran