title: Postnatal epigenetic modification of glucocorticoid receptor gene in preterm infants: a prospective cohort study.
abstract: OBJECTIVE
  To examine the environmental effects on cytosine methylation of preterm infant's DNA, because early life experiences are considered to influence the physiological and mental health of an individual through epigenetic modification of DNA.
  
  
  METHODS
  A prospective cohort study, comparison of epigenetic differences in the glucocorticoid receptor (GR) gene between healthy term and preterm infants.
  
  
  METHODS
  Neonatal Intensive Care Unit in a Japanese University Hospital.
  
  
  METHODS
  A cohort of 40 (20 term and 20 preterm) infants was recruited on the day of birth, and peripheral blood was obtained from each infant at birth and on postnatal day 4.
  
  
  METHODS
  The methylation rates in the 1-F promoter region of the GR gene using the Mquant method.
  
  
  RESULTS
  The methylation rate increased significantly between postnatal days 0 and 4 in preterm infants but remained stable in term infants. Thus, the methylation rate was significantly higher in preterm than in term infants at postnatal day 4. Several perinatal parameters were significantly correlated with this change in the methylation rate. Logistic regression analysis revealed that methylation rates at postnatal day 4 predicted the occurrence of later complications that required glucocorticoid administration during the neonatal period. No gene polymorphism was detected within the GR promoter region analysed.
  
  
  CONCLUSIONS
  Although further large-scale studies are needed to detect the environmental factors that explain the difference in epigenetic modification among infants after birth, our data show that the postnatal environment influences epigenetic programming of GR expression through methylation of the GR gene promoter in premature infants, which may result in relative glucocorticoid insufficiency during the postnatal period.
authors.affiliation: Perinatal Medical Center, Juntendo University Urayasu Hospital, Urayasu, Chiba, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Kantake
authors.affiliation: Institute for Environmental & Gender-specific Medicine, Juntendo University Graduate School of Medicine, Urayasu, Chiba, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshitake
authors.affiliation: Department of Health Information Management, Yamagata Saisei Hospital, Yamagata, Yamagata, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ishikawa
authors.affiliation: Institute for Environmental & Gender-specific Medicine, Juntendo University Graduate School of Medicine, Urayasu, Chiba, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Araki
authors.affiliation: Department of Pediatrics, Juntendo University Graduate School of Medicine, Bunkyo, Tokyo, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D015331:Cohort Studies; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D059647:Gene-Environment Interaction; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D011446:Prospective Studies; D011965:Receptors, Glucocorticoid / Q000235:genetics*
chemical_list: D011965:Receptors, Glucocorticoid
keywords: MOLECULAR BIOLOGY; NEONATOLOGY