title: Photodynamic therapy with di-l-arginine protoporphyrinate on WiDr human colon adenocarcinoma xenografts in athymic nude mice.
abstract: The fluorescence kinetics of a new photosensitizer for photodynamic therapy, di-l-arginine protoporphyrinate (PP(Arg)2), was studied in the skin of healthy mice. Furthermore, induction of necrosis in WiDr human colon adenocarcinoma xenografts in athymic nude mice was studied after photodynamic therapy (PDT) with PP(Arg)2. After intravenous administration of PP(Arg)2 maximal fluorescence was reached after 72h in normal mouse skin. Complete elimination of the drug from the mouse skin was not found even after 32 days. Exposure of WiDr tumours in mice to red light (λ=632nm, fluence 150J/cm(2), fluence rate 250mW/cm(2)) 24 and 72h after intravenous administration of 10mg/kg of PP(Arg)2 caused extensive tumour necrosis. Epidermal damage and infiltration of inflammatory cells was seen 24h after light exposure but not after 72h.
authors.affiliation: Department of Radiation Biology, Institute for Cancer Research, Rikshospitalet-Radiumhospitalet Medical Center, Montebello 0-310 Oslo, Norway.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bugaj
authors.affiliation: Department of Radiation Biology, Institute for Cancer Research, Rikshospitalet-Radiumhospitalet Medical Center, Montebello 0-310 Oslo, Norway; Department of Molecular Microbiology and Serology, National Salmonella Centre, Medical University of Gdańsk, ul. Do Studzienki 38, 81-519 Gdańsk, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Kwitniewski
authors.affiliation: Department of Radiation Biology, Institute for Cancer Research, Rikshospitalet-Radiumhospitalet Medical Center, Montebello 0-310 Oslo, Norway.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Iani
authors.affiliation: Department of Radiation Biology, Institute for Cancer Research, Rikshospitalet-Radiumhospitalet Medical Center, Montebello 0-310 Oslo, Norway.
authors.forename: Asta
authors.initials: A
authors.lastname: Juzeniene
authors.affiliation: Department of Radiation Biology, Institute for Cancer Research, Rikshospitalet-Radiumhospitalet Medical Center, Montebello 0-310 Oslo, Norway.
authors.forename: Petras
authors.initials: P
authors.lastname: Juzenas
authors.affiliation: Department of Radiation Biology, Institute for Cancer Research, Rikshospitalet-Radiumhospitalet Medical Center, Montebello 0-310 Oslo, Norway.
authors.forename: Li Wei
authors.initials: LW
authors.lastname: Ma
authors.affiliation: Department of Radiation Biology, Institute for Cancer Research, Rikshospitalet-Radiumhospitalet Medical Center, Montebello 0-310 Oslo, Norway; Institute of Physics, University of Oslo, 0-316 Oslo, Norway.
authors.forename: Johan
authors.initials: J
authors.lastname: Moan