title: Preliminary characterization of mitochondrial genome of Melipona scutellaris, a Brazilian stingless bee.
abstract: Bees are manufacturers of relevant economical products and have a pollinator role fundamental to ecosystems. Traditionally, studies focused on the genus Melipona have been mostly based on behavioral, and social organization and ecological aspects. Only recently the evolutionary history of this genus has been assessed using molecular markers, including mitochondrial genes. Even though these studies have shed light on the evolutionary history of the Melipona genus, a more accurate picture may emerge when full nuclear and mitochondrial genomes of Melipona species become available. Here we present the assembly, annotation, and characterization of a draft mitochondrial genome of the Brazilian stingless bee Melipona scutellaris using Melipona bicolor as a reference organism. Using Illumina MiSeq data, we achieved the annotation of all protein coding genes, as well as the genes for the two ribosomal subunits (16S and 12S) and transfer RNA genes as well. Using the COI sequence as a DNA barcode, we found that M. cramptoni is the closest species to M. scutellaris.
authors.affiliation: Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Avenida Pará 1720, Campus Umuarama, Bloco 2E, Sala 246, 2° Piso, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Manuella Souza
authors.initials: MS
authors.lastname: Silverio
authors.affiliation: Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Avenida Pará 1720, Campus Umuarama, Bloco 2E, Sala 246, 2° Piso, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Vinícius de Rezende
authors.initials: Vde R
authors.lastname: Rodovalho
authors.affiliation: Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Avenida Pará 1720, Campus Umuarama, Bloco 2E, Sala 246, 2° Piso, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Bonetti
authors.affiliation: René Rachou Research Center-Fiocruz, Molecular and Cellular Pathology Laboratory, Avenida Augusto de Lima 1715, Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Guilherme Corrêa
authors.initials: GC
authors.lastname: de Oliveira
authors.affiliation: René Rachou Research Center-Fiocruz, Molecular and Cellular Pathology Laboratory, Avenida Augusto de Lima 1715, Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Sara
authors.initials: S
authors.lastname: Cuadros-Orellana
authors.affiliation: Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Avenida Pará 1720, Campus Umuarama, Bloco 2E, Sala 246, 2° Piso, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Ueira-Vieira
authors.affiliation: Universidade Federal de Uberlândia, Faculdade de Computação, Avenida João Naves de Ávila 2121, Campus Santa Mônica, Bloco 1B, Sala 148, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: Rodrigues dos Santos
mesh_terms: D000818:Animals; D001516:Bees / Q000145:classification* / Q000235:genetics*; D005075:Biological Evolution; D001938:Brazil; D002874:Chromosome Mapping / Q000379:methods*; D054629:Genome, Mitochondrial / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics*; D010865:Pilot Projects; D013045:Species Specificity
chemical_list: D024101:Mitochondrial Proteins