title: Engineering scaffolds integrated with calcium sulfate and oyster shell for enhanced bone tissue regeneration.
abstract: Engineering scaffolds combinging natural biomineral and artificially synthesized material hold promising potential for bone tissue regeneration. In this study, novel bioactive calcium sulfate/oyster shell (CS/OS) composites were prepared. Comparing to CS scaffold, the CS/OS composites with a controllable degradation rate displayed enhanced mineral nodule formation, higher alkaline phosphate (ALP) activity and increased proliferation rate while treated osteocytes. In CS/OS composites group, elevated mRNA levels of key osteogenic genes including bone morphogenetic protein-2 (BMP-2), runt-related transcription factor 2 (Runx2), osterix (Osx), and osteocalcin (OCN) were observed. Furthermore, The up-regulation of BMP-2 and type I collagen (COL-I) was observed for CS/OS composites relative to a CS group. Scaffolds were implanted into critical-sized femur cavity defects in rabbits to investigate the osteogenic capacity of the composites in vivo. The CS/OS scaffolds with proper suitable times and mechanical strength strongly promoted osteogenic tissue regeneration relative to the regeneration capacity of CS scaffolds, as indicated by the results of histological staining. These results suggest that the OS-modified CS engineering scaffolds with improved mechanical properties and bioactivity would facilitate the development of a new strategy for clinic bone defect regeneration.
authors.affiliation: Department of Orthopaedic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University , Hangzhou 310009, Zhejiang China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
authors.forename: Shizhou
authors.initials: S
authors.lastname: Yang
authors.forename: Jianli
authors.initials: J
authors.lastname: Liu
authors.forename: Huazi
authors.initials: H
authors.lastname: Xu
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Shi
authors.forename: Zhongqing
authors.initials: Z
authors.lastname: Lin
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Ying
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.forename: Tiao
authors.initials: T
authors.lastname: Lin
authors.forename: Shigui
authors.initials: S
authors.lastname: Yan
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry; D060105:Animal Shells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry; D001861:Bone Regeneration / Q000502:physiology*; D001842:Bone and Bones; D002118:Calcium / Q000737:chemistry; D002133:Calcium Sulfate / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D050659:Core Binding Factor Alpha 1 Subunit / Q000737:chemistry; D005260:Female; D005269:Femur / Q000473:pathology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D008422:Materials Testing; D010012:Osteogenesis; D010125:Ostreidae; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014945:Wound Healing; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; D012333:RNA, Messenger; D000469:Alkaline Phosphatase; D002118:Calcium; D002133:Calcium Sulfate