title: Proteomic analysis of lung tissue in a rat acute lung injury model: identification of PRDX1 as a promoter of inflammation.
abstract: Acute respiratory distress syndrome (ARDS) remains a high morbidity and mortality disease entity in critically ill patients, despite decades of numerous investigations into its pathogenesis. To obtain global protein expression changes in acute lung injury (ALI) lung tissues, we employed a high-throughput proteomics method to identify key components which may be involved in the pathogenesis of ALI. In the present study, we analyzed lung tissue proteomes of Pseudomonas aeruginosa-induced ALI rats and identified eighteen proteins whose expression levels changed more than twofold as compared to normal controls. In particular, we found that PRDX1 expression in culture medium was elevated by a lipopolysaccharide (LPS) challenge in airway epithelial cells in vitro. Furthermore, overexpression of PRDX1 increased the expression of proinflammatory cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α), whereas knockdown of PRDX1 led to downregulated expression of cytokines induced by LPS. In conclusion, our findings provide a global alteration in the proteome of lung tissues in the ALI rat model and indicate that PRDX1 may play a critical role in the pathogenesis of ARDS by promoting inflammation and represent a novel strategy for the development of new therapies against ALI.
authors.affiliation: State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang West Road, Guangzhou, Guangdong 510120, China ; Intensive Care Unit, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang West Road, Guangzhou, Guangdong 510120, China ; Infection Control Department, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang West Road, Guangzhou, Guangdong 510120, China ; Intensive Care Unit, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang West Road, Guangzhou, Guangdong 510120, China ; Intensive Care Unit, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang West Road, Guangzhou, Guangdong 510120, China ; Intensive Care Unit, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang West Road, Guangzhou, Guangdong 510120, China ; Intensive Care Unit, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Pang
authors.affiliation: State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang West Road, Guangzhou, Guangdong 510120, China ; Intensive Care Unit, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Li
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C489898:Prdx1 protein, rat; D054464:Peroxiredoxins