title: Spider silk for xeno-free long-term self-renewal and differentiation of human pluripotent stem cells.
abstract: Human pluripotent stem cells (hPSCs) can undergo unlimited self-renewal and have the capacity to differentiate into all somatic cell types, and are therefore an ideal source for the generation of cells and tissues for research and therapy. To realize this potential, defined cell culture systems that allow expansion of hPSCs and subsequent controlled differentiation, ideally in an implantable three-dimensional (3D) matrix, are required. Here we mimic spider silk - Nature's high performance material - for the design of chemically defined 2D and 3D matrices for cell culture. The silk matrices do not only allow xeno-free long-term expansion of hPSCs but also differentiation in both 2D and 3D. These results show that biomimetic spider silk matrices enable hPSC culture in a manner that can be applied for experimental and clinical purposes.
authors.affiliation: Department of Neurobiology, Care Sciences and Society (NVS), Karolinska Institutet, Novum 5th floor, 141 86 Stockholm, Sweden.
authors.forename: Siqin
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Neurobiology, Care Sciences and Society (NVS), Karolinska Institutet, Novum 5th floor, 141 86 Stockholm, Sweden; Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, The Biomedical Centre, Box 575, 751 23 Uppsala, Sweden; Institute of Mathematics and Natural Sciences, Tallinn University, Narva mnt 25, 101 20 Tallinn, Estonia.
authors.forename: Jan
authors.initials: J
authors.lastname: Johansson
authors.affiliation: Department of Clinical Sciences, Intervention and Technology, Division of Obstetrics and Gynecology, Karolinska Institutet and Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden.
authors.forename: Pauliina
authors.initials: P
authors.lastname: Damdimopoulou
authors.affiliation: Department of Neuroscience, Karolinska Institutet, Retzius v. 8, 171 77 Stockholm, Sweden.
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Shahsavani
authors.affiliation: Department of Neuroscience, Karolinska Institutet, Retzius v. 8, 171 77 Stockholm, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Falk
authors.affiliation: Department of Clinical Sciences, Intervention and Technology, Division of Obstetrics and Gynecology, Karolinska Institutet and Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden.
authors.forename: Outi
authors.initials: O
authors.lastname: Hovatta
authors.affiliation: Department of Neurobiology, Care Sciences and Society (NVS), Karolinska Institutet, Novum 5th floor, 141 86 Stockholm, Sweden; Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, The Biomedical Centre, Box 575, 751 23 Uppsala, Sweden. Electronic address: anna.rising@ki.se.
authors.forename: Anna
authors.initials: A
authors.lastname: Rising
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology*; D047011:Silk / Q000494:pharmacology*; D013112:Spiders
chemical_list: D047011:Silk
keywords: Biomaterial; Chemically defined; Functionalized materials; Human embryonic stem cells; Human induced pluripotent stem cells; Scaffold