title: Expression of high mobility group box 1 in inflamed dental pulp and its chemotactic effect on dental pulp cells.
abstract: High mobility group box 1 protein (HMGB1) is a chromatin protein which can be released extracellularly, eliciting a pro-inflammatory response and promoting tissue repair process. This study aimed to examine the expression and distribution of HMGB1 and its receptor RAGE in inflamed dental pulp tissues, and to assess its effects on proliferation, migration and cytoskeleton of cultured human dental pulp cells (DPCs). Our data demonstrated that cytoplasmic expression of HMGB1 was observed in inflamed pulp tissues, while HMGB1 expression was confined in the nuclei in healthy dental pulp. The mRNA expression of HMGB1 and RAGE were significantly increased in inflamed pulps. In in vitro cultured DPCs, expression of HMGB1 in both protein and mRNA level was up-regulated after treated with lipopolysaccharide (LPS). Exogenous HMGB1 enhanced DPCs migration in a dose-dependent manner and induced the reorganization of f-actin in DPCs. Our results suggests that HMGB1 are not only involved in the process of dental pulp inflammation, but also play an important role in the recruitment of dental pulp stem cells, promoting pulp repair and regeneration.
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, PR China; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia. Electronic address: xufang.zhang@student.qut.edu.au.
authors.forename: Xufang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, PR China. Electronic address: jianghw@163.com.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, PR China. Electronic address: gongqmei@gmail.com.
authors.forename: Qimei
authors.initials: Q
authors.lastname: Gong
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia. Electronic address: c3.fan@student.qut.edu.au.
authors.forename: Chen
authors.initials: C
authors.lastname: Fan
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, PR China. Electronic address: enu0701@163.com.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, PR China. Electronic address: lingjq@mail.sysu.edu.cn.
authors.forename: Junqi
authors.initials: J
authors.lastname: Ling
mesh_terms: D001483:Base Sequence; D049109:Cell Proliferation; D002633:Chemotaxis; D017931:DNA Primers; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D024243:HMGB1 Protein / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D024243:HMGB1 Protein
keywords: Cell migration; Dental pulp cells; High mobility group box 1; Inflammation; Receptor for advanced glycation end products