title: Solution-based indirect affinity selection mass spectrometry--a general tool for high-throughput screening of pharmaceutical compound libraries.
abstract: We show here that an automated solution-based affinity selection mass spectrometry (ASMS) system can be built exclusively from commercially available parts. The value of this technology lies in the throughput (~1 × 10(5) compounds/day) coupled with a low hit rate. The system, being a binding assay, requires little development time yielding a fast timeline between target availability and hit identification. In addition, the use of exact mass simplifies the hit identification. We demonstrate this system using carbonic anhydrase as the target and a library of 144,000 proprietary compounds.
authors.affiliation: Center of Chemistry Innovation and Excellence and ‡Research Informatics, Pfizer, Inc. , Groton, Connecticut 06340, United States.
authors.forename: Thomas N
authors.initials: TN
authors.lastname: O'Connell
authors.forename: Jason
authors.initials: J
authors.lastname: Ramsay
authors.forename: Steven F
authors.initials: SF
authors.lastname: Rieth
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Shapiro
authors.forename: Justin G
authors.initials: JG
authors.lastname: Stroh
mesh_terms: D057166:High-Throughput Screening Assays; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D014462:Ultrafiltration
chemical_list: D004364:Pharmaceutical Preparations