title: Influence of Populus genotype on gene expression by the wood decay fungus Phanerochaete chrysosporium.
abstract: We examined gene expression patterns in the lignin-degrading fungus Phanerochaete chrysosporium when it colonizes hybrid poplar (Populus alba × tremula) and syringyl (S)-rich transgenic derivatives. A combination of microarrays and liquid chromatography-tandem mass spectrometry (LC-MS/MS) allowed detection of a total of 9,959 transcripts and 793 proteins. Comparisons of P. chrysosporium transcript abundance in medium containing poplar or glucose as a sole carbon source showed 113 regulated genes, 11 of which were significantly higher (>2-fold, P < 0.05) in transgenic line 64 relative to the parental line. Possibly related to the very large amounts of syringyl (S) units in this transgenic tree (94 mol% S), several oxidoreductases were among the upregulated genes. Peptides corresponding to a total of 18 oxidoreductases were identified in medium consisting of biomass from line 64 or 82 (85 mol% S) but not in the parental clone (65 mol% S). These results demonstrate that P. chrysosporium gene expression patterns are substantially influenced by lignin composition.
authors.affiliation: USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA.
authors.forename: Jill
authors.initials: J
authors.lastname: Gaskell
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison, Madison, Wisconsin, USA.
authors.forename: Amber
authors.initials: A
authors.lastname: Marty
authors.affiliation: USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Mozuch
authors.affiliation: USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Kersten
authors.affiliation: Genetics and Biotechnology Center, University of Wisconsin, Madison, Madison, Wisconsin, USA.
authors.forename: Sandra
authors.initials: S
authors.lastname: Splinter BonDurant
authors.affiliation: Genetics and Biotechnology Center, University of Wisconsin, Madison, Madison, Wisconsin, USA.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Sabat
authors.affiliation: Department of Biochemistry, and Department of Energy Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, Madison, Wisconsin, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Azarpira
authors.affiliation: Department of Biochemistry, and Department of Energy Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, Madison, Wisconsin, USA.
authors.forename: John
authors.initials: J
authors.lastname: Ralph
authors.affiliation: Department of Wood Science, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Skyba
authors.affiliation: Department of Wood Science, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Shawn D
authors.initials: SD
authors.lastname: Mansfield
authors.affiliation: Department of Plant Pathology, University of Minnesota, Saint Paul, Minnesota, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Blanchette
authors.affiliation: USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA dcullen@wisc.edu.
authors.forename: Dan
authors.initials: D
authors.lastname: Cullen
mesh_terms: D002244:Carbon / Q000378:metabolism; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005838:Genotype; D008031:Lignin / Q000378:metabolism; D046228:Microarray Analysis; D020075:Phanerochaete / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D032107:Populus / Q000235:genetics*; D053719:Tandem Mass Spectrometry; D014934:Wood / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D003470:Culture Media; D002244:Carbon; D008031:Lignin