title: Impact of selenium supply on Se-methylselenocysteine and glucosinolate accumulation in selenium-biofortified Brassica sprouts.
abstract: Brassica sprouts are widely marketed as functional foods. Here we examined the effects of Se treatment on the accumulation of anticancer compound Se-methylselenocysteine (SeMSCys) and glucosinolates in Brassica sprouts. Cultivars from the six most extensively consumed Brassica vegetables (broccoli, cauliflower, green cabbage, Chinese cabbage, kale, and Brussels sprouts) were used. We found that Se-biofortified Brassica sprouts all were able to synthesize significant amounts of SeMSCys. Analysis of glucosinolate profiles revealed that each Brassica crop accumulated different types and amounts of glucosinolates. Cauliflower sprouts had high total glucosinolate content. Broccoli sprouts contained high levels of glucoraphanin, a precursor for potent anticancer compound. Although studies have reported an inverse relationship between accumulation of Se and glucosinolates in mature Brassica plants, Se supply generally did not affect glucosinolate accumulation in Brassica sprouts. Thus, Brassica vegetable sprouts can be biofortified with Se for the accumulation of SeMSCys without negative effects on chemopreventive glucosinolate contents.
authors.affiliation: Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA; Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG 37200-000, Brazil; Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Fabricio William
authors.initials: FW
authors.lastname: Avila
authors.affiliation: Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG 37200-000, Brazil.
authors.forename: Valdemar
authors.initials: V
authors.lastname: Faquin
authors.affiliation: Instituto Tecnológico Vale, Belo Horizonte, MG 30140-130, Brazil.
authors.forename: Silvio Junio
authors.initials: SJ
authors.lastname: Ramos
authors.affiliation: Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG 37200-000, Brazil.
authors.forename: Luiz Roberto G
authors.initials: LR
authors.lastname: Guilherme
authors.affiliation: Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Theodore W
authors.initials: TW
authors.lastname: Thannhauser
authors.affiliation: Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA; Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, USA. Electronic address: ll37@cornell.edu.
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D016588:Anticarcinogenic Agents / Q000737:chemistry; D001937:Brassica / Q000737:chemistry*; D002853:Chromatography, Liquid; D005527:Food, Fortified / Q000032:analysis; D005961:Glucosinolates / Q000737:chemistry*; D007096:Imidoesters / Q000737:chemistry*; D010091:Oximes; D012643:Selenium / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry; D017279:Selenocysteine / Q000031:analogs & derivatives* / Q000737:chemistry; D013454:Sulfoxides; D013455:Sulfur / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D014675:Vegetables
chemical_list: D000596:Amino Acids; D016588:Anticarcinogenic Agents; D005961:Glucosinolates; D007096:Imidoesters; D010091:Oximes; D018036:Selenium Compounds; D013454:Sulfoxides; D017279:Selenocysteine; D013455:Sulfur; D012643:Selenium; C119494:glucoraphanin; C002979:selenomethylselenocysteine
keywords: Brassica sprouts; Function food; Glucosinolates; Se-methylselenocysteine; Selenium