title: Successful diagnosis of tuberculous lymphadenitis by loop-mediated isothermal amplification of cutaneous samples from an ulcerated surface lesion: a case report.
abstract: BACKGROUND
  Tuberculous lymphadenitis is the most frequent form of extrapulmonary tuberculous. Although nucleic acid amplification assays such as polymerase chain reaction have recently become mainstream techniques for diagnosing tuberculous lymphadenitis, they are still not routinely performed in developing countries because of their high costs and complicated procedures.
  
  
  METHODS
  We describe a case of tuberculous lymphadenitis in a 79-year-old Japanese man who had been on continuous hemodialysis for end-stage renal disease. We employed loop-mediated isothermal amplification and the procedure for ultrarapid extraction to develop a fast and easy-to-perform procedure for diagnosing tuberculous lymphadenitis.
  
  
  CONCLUSIONS
  The commercially available loop-mediated isothermal amplification assay kit and a rapid purification procedure enabled us to identify and amplify a Mycobacterium tuberculosis-specific gene within just 1.5 hours.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kawano
authors.affiliation: Division of Infectious Diseases and Pulmonary Medicine, Department of Internal Medicine, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan. tmaeda@ndmc.ac.jp.
authors.forename: Takuya
authors.initials: T
authors.lastname: Maeda
authors.forename: Junichi
authors.initials: J
authors.lastname: Watanabe
authors.forename: Yuji
authors.initials: Y
authors.lastname: Fujikura
authors.forename: Kei
authors.initials: K
authors.lastname: Mikita
authors.forename: Yu
authors.initials: Y
authors.lastname: Hara
authors.forename: Soichiro
authors.initials: S
authors.lastname: Kanoh
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kimura
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Miyahira
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kawana
mesh_terms: D000368:Aged; D000995:Antitubercular Agents / Q000627:therapeutic use; D044963:Biopsy, Fine-Needle; D003937:Diagnosis, Differential; D006801:Humans; D016867:Immunocompromised Host; D007676:Kidney Failure, Chronic / Q000628:therapy; D008297:Male; D009333:Neck; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D006435:Renal Dialysis; D014057:Tomography, X-Ray Computed; D014388:Tuberculosis, Lymph Node / Q000175:diagnosis* / Q000188:drug therapy
chemical_list: D000995:Antitubercular Agents