title: Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials.
abstract: A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volume (P/V) and constant tip speed. Moreover, lipase immobilization on different materials was also investigated. Lipase production was performed using liquid-state bioconversion of POME as the medium and Candida cylindracea as the inoculum. The fermentation medium was composed of 1% total suspended solids (TSS) of POME, 0.5% (w/v) peptone, 0.7% (v/v) Tween-80, and 2.2% inoculum. The medium composition was decided on the basis of the medium optimization results of a previous study. The fermentation was carried out for 48 h at 30°C and pH 6. The maximum lipase production was 5.72U/mL and 21.34 U/mL, obtained from the scale-up strategies of constant tip speed and P/V, respectively. Four accessible support materials were screened for their potential use in immobilization. The most suitable support material was found to be activated carbon, with a maximum immobilization of 94%.
authors.affiliation: Bioenvironmental Engineering Research Centre (BERC), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia (IIUM).
authors.forename: Devi Ratna
authors.initials: DR
authors.lastname: Asih
authors.forename: Md Zahangir
authors.initials: MZ
authors.lastname: Alam
authors.forename: Zahangir
authors.initials: Z
authors.lastname: Alam
authors.forename: Md Noor
authors.initials: MN
authors.lastname: Salleh
authors.forename: Noor
authors.initials: N
authors.lastname: Salleh
authors.forename: Aliyu
authors.initials: A
authors.lastname: Salihu
mesh_terms: D019149:Bioreactors; D002175:Candida; D002606:Charcoal; D004800:Enzymes, Immobilized / Q000138:chemical synthesis*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000138:chemical synthesis*; D000073878:Palm Oil; D010461:Peptones; D010938:Plant Oils / Q000737:chemistry*; D011136:Polysorbates; D013696:Temperature; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D010461:Peptones; D010938:Plant Oils; D011136:Polysorbates; D002606:Charcoal; D000073878:Palm Oil; D008049:Lipase