title: A proteomic approach for the involvement of the GAPDH in Alzheimer disease in the blood of Moroccan FAD cases.
abstract: Several articles have highlighted the potential involvement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in neurodegeneration by showing a non-glycolytic activity of GAPDH specifically in the brains of subjects with Alzheimer's disease (AD). The novel aim of this study was to elucidate the critical role of GAPDH and its interaction with β-amyloid in the blood of Moroccan patients with familial AD (FAD) carrying presenilin mutations and in sporadic late onset AD (LOAD). Our results show a significant decrease in the activity of GAPDH in blood samples from patients with FAD as compared to sporadic cases and healthy controls. The expression level of GAPDH in brain specimens from mutant tau transgenic mice and patients with FAD was unchanged as compared to healthy controls. In contrast, the expression level of GAPDH in blood samples from mutant tau transgenic mice and patients with FAD was decreased as compared to sporadic cases and healthy controls. Moreover, there is an accumulation of β-amyloid aggregates in the blood samples of patients with FAD and an increase in amyloid fibrils in both the blood and brain samples of these patients. Our study adds new insight to previous ones by showing the involvement of GAPDH in AD, which may influence the pathogenesis of this neurodegenerative disease.
authors.affiliation: Laboratory of Medical Genetics and Molecular Pathology, Faculty of Medicine and Pharmacy, University Hassan II, 19 Rue Tarik Ibnou Ziad, B.P. 9154, Casablanca, 20000, Morocco, elkadmiri1979@gmail.com.
authors.forename: Nadia
authors.initials: N
authors.lastname: El Kadmiri
authors.forename: Raquel
authors.initials: R
authors.lastname: Cuardos
authors.forename: Bouchra
authors.initials: B
authors.lastname: El Moutawakil
authors.forename: Ilham
authors.initials: I
authors.lastname: Slassi
authors.forename: Jesus
authors.initials: J
authors.lastname: Avila
authors.forename: Sellama
authors.initials: S
authors.lastname: Nadifi
authors.forename: Ahmed
authors.initials: A
authors.lastname: Hachem
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Soukri
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000201:enzymology* / Q000235:genetics; D016229:Amyloid beta-Peptides / Q000097:blood / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000097:blood / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D053763:Presenilins / Q000235:genetics; D040901:Proteomics
chemical_list: D016229:Amyloid beta-Peptides; D053763:Presenilins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases