title: Targeted genome modification via triple helix formation.
abstract: Triplex-forming oligonucleotides (TFOs) are capable of coordinating genome modification in a targeted, site-specific manner, causing mutagenesis or even coordinating homologous recombination events. Here, we describe the use of TFOs such as peptide nucleic acids for targeted genome modification. We discuss this method and its applications and describe protocols for TFO design, delivery, and evaluation of activity in vitro and in vivo.
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
authors.forename: Adele S
authors.initials: AS
authors.lastname: Ricciardi
authors.forename: Nicole A
authors.initials: NA
authors.lastname: McNeer
authors.forename: Kavitha K
authors.initials: KK
authors.lastname: Anandalingam
authors.forename: W Mark
authors.initials: WM
authors.lastname: Saltzman
authors.forename: Peter M
authors.initials: PM
authors.lastname: Glazer
mesh_terms: D000818:Animals; D015870:Gene Expression; D018390:Gene Targeting / Q000379:methods*; D017930:Genes, Reporter; D016678:Genome / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D051379:Mice; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D020135:Peptide Nucleic Acids; D011995:Recombination, Genetic
chemical_list: D009841:Oligonucleotides; D020135:Peptide Nucleic Acids