title: Protective effect of veratric acid on lipopolysaccharide-induced acute lung injury in mice.
abstract: Veratric acid, a simple benzoic acid derived from plants and fruits, has been reported to exhibit antibacterial, anti-inflammatory and other important therapeutic activities. The aim of this study was to detect the effects of veratric acid on LPS-induced acute lung injury and to investigate the effects of veratric acid on NF-κB signaling pathway. Male BALB/c mice were pretreated with dexamethasone or veratric acid 1h before intranasal instillation of LPS. 7h after LPS administration, the myeloperoxidase in lung tissues, lung wet/dry weight ratio and inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The effects of veratric acid on pro-inflammatory cytokines and signal pathways were analyzed by enzyme-linked immunosorbent assay (ELISA) and Western blotting. The results showed that veratric acid inhibited LPS-induced TNF-α, IL-6 and IL-1β production in a dose dependent manner. It was also observed that veratric acid attenuated lung histopathologic changes. The wet/dry weight ratio of lungs and the number of total cells, neutrophils, macrophages in the BALF were all decreased. Furthermore, veratric acid inhibited the phosphorylation of NF-κB p65 and IκB. These results indicate that veratric acid inhibits NF-κB signaling pathways to attenuate inflammatory injury induced by LPS. Veratric acid may be a potential therapeutic reagent for acute lung injury treatment.
authors.affiliation: Department of Thoracic Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Ran
authors.affiliation: Department of Neurobiology, Basic Medical College, China Medical University, Shenyang 110004, Liaoning, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Chao
authors.affiliation: Department of Thoracic Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jun-Gang
authors.affiliation: Department of Thoracic Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning, China.
authors.forename: Han
authors.initials: H
authors.lastname: Yun
authors.affiliation: Department of Thoracic Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Kuan-Bing
authors.affiliation: Department of Thoracic Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning, China. Electronic address: shiwenjunyl@163.com.
authors.forename: Shi
authors.initials: S
authors.lastname: Wen-Jun
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D053583:Interleukin-1beta / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008070:Lipopolysaccharides; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D009195:Peroxidase / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D014641:Vanillic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C009333:veratric acid; D009195:Peroxidase; D014641:Vanillic Acid
keywords: Acute lung injury; LPS; NF-κB; Veratric acid