title: [Experimental study on anti-tumor effect of xihuang pill and its immune clearance function].
abstract: OBJECTIVE
  To discuss the anti-tumor effect of Xihuang pill on tumor-bearing rats and its effect on the immune clearance function of tumor-bearing organisms.
  
  
  METHODS
  Walker256 tumor cells were adopted to establish the tumor-bearing rat model. The rats were randomly divided into five groups: the normal control group, the model control group, the lentinan group and Xihuang pill low dose, middle dose and high dose groups, with 10 rats in each group, and continuously treated and given drugs for 14 d after modeling. Blood and tumors were collected from abdominal aorta to calculate the tumor inhibition rate. The content of CD3+, CD4+, CD8+ T cells and adhesion molecule B7-1 (CD80) in peripheral blood were detected by flow cytometry (FCM). The expressions of IL-2 and IFN-gamma in were determined by ELISA.
  
  
  RESULTS
  The tumor inhibition rate of the Xihuang pill high dose group was 33. 1 percent. Compared with the model group, the Xihuang pill large dose group showed significantly low IL-2, IFN-gamma, CD3+, CD4+, B7-1 in peripheral blood, with statistical significance in their differences (P < 0.05).
  
  
  CONCLUSIONS
  Xihuang pill could show its anti-tumor effect by enhancing the immune clearance function and increasing IL-2, IFN-gamma, CD3+ T, CD4+ T, B7-1 in peripheral blood.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Yi-Yao
authors.initials: YY
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Shuo
authors.initials: S
authors.lastname: Guan
authors.forename: Chang-Qian
authors.initials: CQ
authors.lastname: Zeng
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Gao
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Liang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007107:Immune System / Q000187:drug effects; D007155:Immunologic Factors / Q000008:administration & dosage*; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D018122:B7-1 Antigen; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D007376:Interleukin-2; D007371:Interferon-gamma