title: A comprehensive protocol for manual segmentation of the medial temporal lobe structures.
abstract: The present paper describes a comprehensive protocol for manual tracing of the set of brain regions comprising the medial temporal lobe (MTL): amygdala, hippocampus, and the associated parahippocampal regions (perirhinal, entorhinal, and parahippocampal proper). Unlike most other tracing protocols available, typically focusing on certain MTL areas (e.g., amygdala and/or hippocampus), the integrative perspective adopted by the present tracing guidelines allows for clear localization of all MTL subregions. By integrating information from a variety of sources, including extant tracing protocols separately targeting various MTL structures, histological reports, and brain atlases, and with the complement of illustrative visual materials, the present protocol provides an accurate, intuitive, and convenient guide for understanding the MTL anatomy. The need for such tracing guidelines is also emphasized by illustrating possible differences between automatic and manual segmentation protocols. This knowledge can be applied toward research involving not only structural MRI investigations but also structural-functional colocalization and fMRI signal extraction from anatomically defined ROIs, in healthy and clinical groups alike.
authors.affiliation: Psychology Department, University of Illinois Urbana-Champaign.
authors.forename: Matthew
authors.initials: M
authors.lastname: Moore
authors.affiliation: Psychology Department, University of Illinois Urbana-Champaign.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Psychology Department, University of Illinois Urbana-Champaign.
authors.forename: Sarah
authors.initials: S
authors.lastname: Woo
authors.affiliation: Psychology Department, University of Illinois Urbana-Champaign.
authors.forename: Dylan
authors.initials: D
authors.lastname: O'Hearn
authors.affiliation: Neuroscience Program, University of Illinois Urbana-Champaign; Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign.
authors.forename: Alexandru D
authors.initials: AD
authors.lastname: Iordan
authors.affiliation: Psychology Department, University of Illinois Urbana-Champaign.
authors.forename: Sanda
authors.initials: S
authors.lastname: Dolcos
authors.affiliation: Psychology Department, University of Illinois Urbana-Champaign; Neuroscience Program, University of Illinois Urbana-Champaign; Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign; fdolcos@illinois.edu.
authors.forename: Florin
authors.initials: F
authors.lastname: Dolcos
mesh_terms: D000328:Adult; D000679:Amygdala / Q000033:anatomy & histology; D002540:Cerebral Cortex / Q000033:anatomy & histology; D005260:Female; D006624:Hippocampus / Q000033:anatomy & histology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D013702:Temporal Lobe / Q000033:anatomy & histology*; D055815:Young Adult