title: The ameliorative effect of propolis against methoxychlor induced ovarian toxicity in rat.
abstract: A study was designed to evaluate ameliorative effect of propolis against methoxychlor (MXC) induced ovarian toxicity in rat. The organochlorine pesticide (MXC) is a known endocrine disruptor with estrogenic, anti-estrogenic, and anti-androgenic properties. To investigate whether chronic exposure to MXC could cause ovarian dysfunction, two groups of Sprague-Dawley adult female rats were exposed to MXC alone in a dose of 200mg/kg, twice/weekly, orally or MXC dose as previous plus propolis in a dose of 200mg/l/day, in drinking water for 10 months. Another two groups of rat were given corn oil (control) or propolis. Multiple reproductive parameters, ovarian weight, serum hormone levels, ovarian oxidative status and ovarian morphology were examined. In MXC-exposed group, there is a significant decrease in body and ovarian weight vs. control. MXC decreases serum estradiol and progesterone levels. A significant increase in the levels of lipid peroxidation was obtained while a significant decrease of the total antioxidant was recorded. Ovarian histopathology showed primary, secondary and vesicular follicles displaying an atretic morphology. Increase in the ovarian surface epithelium height accompanied with vacuolated, pyknotic oocytes were obtained. The previous toxic effects were neutralized by the administration of propolis in MXC+propolis group. The present results suggest that propolis may be effective in decreasing of MXC-induced ovarian toxicity in rat.
authors.affiliation: Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Assuit University, Egypt. Electronic address: medicine1971@yahoo.com.
authors.forename: Eman E
authors.initials: EE
authors.lastname: El-Sharkawy
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Assuit University, Egypt.
authors.forename: Amany O G
authors.initials: AO
authors.lastname: Kames
authors.affiliation: Animal Health Research Institute, Assuit, Egypt.
authors.forename: S M
authors.initials: SM
authors.lastname: Sayed
authors.affiliation: Animal Health Research Institute, Assuit, Egypt.
authors.forename: Neveen A E L
authors.initials: NA
authors.lastname: Nisr
authors.affiliation: Animal Health Research Institute, Assuit, Egypt.
authors.forename: Nahed M
authors.initials: NM
authors.lastname: Wahba
authors.affiliation: Animal Health Research Institute, Assuit, Egypt.
authors.forename: Walaa M
authors.initials: WM
authors.lastname: Elsherif
authors.affiliation: Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Assuit University, Egypt.
authors.forename: Allam M
authors.initials: AM
authors.lastname: Nafady
authors.affiliation: Animal Health Research Institute, Assuit, Egypt.
authors.forename: M M
authors.initials: MM
authors.lastname: Abdel-Hafeez
authors.affiliation: Department of Animal Medicine and Clinical Laboratory Diagnosis, Faculty of Veterinary Medicine, Assuit University, Egypt.
authors.forename: A A
authors.initials: AA
authors.lastname: Aamer
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D007306:Insecticides / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008731:Methoxychlor / Q000633:toxicity*; D010053:Ovary / Q000187:drug effects / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects; D011429:Propolis / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000890:Anti-Infective Agents; D052244:Endocrine Disruptors; D007306:Insecticides; D011429:Propolis; D008731:Methoxychlor
keywords: Methoxychlor; Ovarian follicles; Propolis; Steroid hormones, Oxidative damage