title: Effects of doxycycline on clinical, microbiological and immunological parameters in well-controlled diabetes type-2 patients with periodontal disease: a randomized, controlled clinical trial.
abstract: OBJECTIVE
  To evaluate the clinical, microbiological and immunological effects of systemic doxycycline as an adjunct to scaling and root planing (SRP) in chronic periodontitis patients with well-controlled type 2 diabetes.
  
  
  METHODS
  Sixty-six patients compliant to oral hygiene (Hygiene Index <20%) allocated to either a test (systemic doxycycline for 21 days) or a control (placebo) group participated in the present randomized controlled trial (RCT). Clinical assessments were recorded at baseline, 3 and 6 months after therapy and included clinical attachment level (CAL), set as the primary outcome of the study, probing pocket depth (PPD), recession (RE) and bleeding on probing (BOP). At the same time points, counts of 15 subgingival species were evaluated by "checkerboard" DNA-DNA hybridization, gingival crevicular fluid samples were analysed for matrix metalloproteinase-8 (MMP-8) by ELISA and HbA1c levels were determined. Comparisons between and within groups were performed by non-parametric tests (Mann-Whitney, Wilcoxon signed-ranks and z-test for proportions with Bonferroni corrections) at the 0.05 level.
  
  
  RESULTS
  No major differences were noticed in clinical and microbiological parameters of periodontal disease or levels of MMP-8 between the two groups.
  
  
  CONCLUSIONS
  Adjunctive systemic doxycycline does not seem to significantly enhance the effects of SRP in well-controlled diabetes type 2 patients.
authors.affiliation: Department of Preventive Dentistry, Periodontology and Implant Biology, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Lazaros
authors.initials: L
authors.lastname: Tsalikis
authors.forename: Dimitra
authors.initials: D
authors.lastname: Sakellari
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Dagalis
authors.forename: Panagiota
authors.initials: P
authors.lastname: Boura
authors.forename: Antonios
authors.initials: A
authors.lastname: Konstantinidis
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D058491:Bacterial Load / Q000187:drug effects; D055113:Chronic Periodontitis / Q000382:microbiology / Q000628:therapy*; D003131:Combined Modality Therapy; D003773:Dental Plaque / Q000382:microbiology; D012534:Dental Scaling / Q000379:methods; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000517:prevention & control*; D004311:Double-Blind Method; D004318:Doxycycline / Q000627:therapeutic use*; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D005884:Gingival Hemorrhage / Q000382:microbiology / Q000628:therapy; D005889:Gingival Recession / Q000382:microbiology / Q000628:therapy; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000032:analysis; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000382:microbiology / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000382:microbiology / Q000628:therapy; D010919:Placebos; D016745:Root Planing / Q000379:methods; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D004318:Doxycycline; D006442:Glycated Hemoglobin; D020784:Matrix Metalloproteinase 8; D010919:Placebos; C488762:MMP8 protein, human
keywords: diabetes type-2; doxycycline; periodontal therapy; randomized controlled trial