title: Short communication: antiviral activity of subcritical water extract of Brassica juncea against influenza virus A/H1N1 in nonfat milk.
abstract: Subcritical water extract (SWE) of Brassica juncea was studied for antiviral effects against influenza virus A/H1N1 and for the possibility of application as a nonfat milk supplement for use as an "antiviral food." At maximum nontoxic concentrations, SWE had higher antiviral activity against influenza virus A/H1N1 than n-hexane, ethanol, or hot water (80°C) extracts. Addition of 0.5mg/mL of B. juncea SWE to culture medium led to 50.35% cell viability (% antiviral activity) for Madin-Darby canine kidney cells infected with influenza virus A/H1N1. Nonfat milk supplemented with 0.28mg/mL of B. juncea SWE showed 39.62% antiviral activity against influenza virus A/H1N1. Thus, the use of B. juncea SWE as a food supplement might aid in protection from influenza viral infection.
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: N-K
authors.initials: NK
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: J-H
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: S-M
authors.initials: SM
authors.lastname: Lim
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: K A
authors.initials: KA
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Y B
authors.initials: YB
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: P-S
authors.initials: PS
authors.lastname: Chang
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Republic of Korea; Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: hdpaik@konkuk.ac.kr.
authors.forename: H-D
authors.initials: HD
authors.lastname: Paik
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004285:Dogs; D005503:Food Additives / Q000494:pharmacology; D006586:Hexanes; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D061985:Madin Darby Canine Kidney Cells / Q000187:drug effects; D008892:Milk / Q000737:chemistry*; D009149:Mustard Plant / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D014867:Water
chemical_list: D000998:Antiviral Agents; D005503:Food Additives; D006586:Hexanes; D010936:Plant Extracts; D014867:Water; C026385:n-hexane
keywords: Brassica juncea; antiviral activity; influenza virus; subcritical water extraction