title: Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity.
abstract: We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.
authors.forename: Sébastien A
authors.initials: SA
authors.lastname: Smallwood
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.|Wellcome Trust Sanger Institute, Cambridge, UK.
authors.forename: Heather J
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK.
authors.forename: Christof
authors.initials: C
authors.lastname: Angermueller
authors.affiliation: Bioinformatics Group, Babraham Institute, Cambridge, UK.
authors.forename: Felix
authors.initials: F
authors.lastname: Krueger
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.
authors.forename: Heba
authors.initials: H
authors.lastname: Saadeh
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.
authors.forename: Julian
authors.initials: J
authors.lastname: Peat
authors.affiliation: Bioinformatics Group, Babraham Institute, Cambridge, UK.
authors.forename: Simon R
authors.initials: SR
authors.lastname: Andrews
authors.affiliation: European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK.
authors.forename: Oliver
authors.initials: O
authors.lastname: Stegle
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.|Centre for Trophoblast Research, University of Cambridge, Cambridge, UK.|Wellcome Trust Sanger Institute, Cambridge, UK.
authors.forename: Wolf
authors.initials: W
authors.lastname: Reik
authors.affiliation: Epigenetics Programme, Babraham Institute, Cambridge, UK.|Centre for Trophoblast Research, University of Cambridge, Cambridge, UK.
authors.forename: Gavin
authors.initials: G
authors.lastname: Kelsey
mesh_terms: D000818:Animals; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D016678:Genome; D051379:Mice; D013447:Sulfites / Q000737:chemistry*
chemical_list: D013447:Sulfites; C042345:hydrogen sulfite