title: Photosynthetic acclimation of Nannochloropsis oculata investigated by multi-wavelength chlorophyll fluorescence analysis.
abstract: Multi-wavelength chlorophyll fluorescence analysis was utilised to examine the photosynthetic efficiency of the biofuel-producing alga Nannochloropsis oculata, grown under two light regimes; low (LL) and high (HL) irradiance levels. Wavelength dependency was evident in the functional absorption cross-section of Photosystem II (σII(λ)), absolute electron transfer rates (ETR(II)), and non-photochemical quenching (NPQ) of chlorophyll fluorescence in both HL and LL cells. While σII(λ) was not significantly different between the two growth conditions, HL cells upregulated ETR(II) 1.6-1.8-fold compared to LL cells, most significantly in the wavelength range of 440-540 nm. This indicates preferential utilisation of blue-green light, a highly relevant spectral region for visible light in algal pond conditions. Under these conditions, the HL cells accumulated saturated fatty acids, whereas polyunsaturated fatty acids were more abundant in LL cells. This knowledge is of importance for the use of N. oculata for fatty acid production in the biofuel industry.
authors.affiliation: Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Broadway, NSW 2007, Australia. Electronic address: Milan.Szabo@uts.edu.au.
authors.forename: Milán
authors.initials: M
authors.lastname: Szabó
authors.affiliation: Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Kieran
authors.initials: K
authors.lastname: Parker
authors.affiliation: Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Supriya
authors.initials: S
authors.lastname: Guruprasad
authors.affiliation: Centre for Forensic Science, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Unnikrishnan
authors.initials: U
authors.lastname: Kuzhiumparambil
authors.affiliation: Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Ross McC
authors.initials: RM
authors.lastname: Lilley
authors.affiliation: Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Bojan
authors.initials: B
authors.lastname: Tamburic
authors.affiliation: Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Martin
authors.initials: M
authors.lastname: Schliep
authors.affiliation: Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Anthony W D
authors.initials: AW
authors.lastname: Larkum
authors.affiliation: Julius-von-Sachs Institut für Biowissenschaften, Universität Würzburg, Julius-von-Sachs Platz 2, 97082 Würzburg, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schreiber
authors.affiliation: Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: John A
authors.initials: JA
authors.lastname: Raven
authors.affiliation: Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Broadway, NSW 2007, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Ralph
mesh_terms: D065966:Absorption, Physicochemical; D000064:Acclimatization; D002734:Chlorophyll / Q000378:metabolism*; D004579:Electron Transport; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005453:Fluorescence; D058086:Microalgae / Q000378:metabolism*; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000378:metabolism; D011789:Quantum Theory
chemical_list: D004952:Esters; D005227:Fatty Acids; D045332:Photosystem II Protein Complex; D002734:Chlorophyll
keywords: Biofuels; Chlorophyll fluorescence; Nannochloropsis spp.; Non-photochemical quenching; Photosynthetic electron transport rate