title: Metabolomics investigation of cutaneous T cell lymphoma based on UHPLC-QTOF/MS.
abstract: OBJECTIVE
  The identification of cutaneous T cell lymphoma (CTCL) biomarkers may serve as a predictor of disease progression and treatment response. The aim of this study was to map potential biomarkers in CTCL plasma.
  
  
  METHODS
  Plasma metabolic perturbations between CTCL cases and healthy individuals were investigated using metabolomics and ultrahigh performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS).
  
  
  RESULTS
  Principal component analysis (PCA) of the spectra showed clear metabolic changes between the two groups. Thirty six potential biomarkers associated with CTCL were found.
  
  
  CONCLUSIONS
  Based on PCA, several biomarkers were determined and further identified by LC/MS/MS analysis. All of these could be potential early markers of CTCL. In addition, we established that heparin as anticoagulant has better pre-treatment results than EDTA with the UHPLC-QTOF/MS approach.
authors.affiliation: School of Pharmacy, Fudan University, Shanghai, China E-mail : yqyu@shmu.edu.cn.
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Zhou
authors.forename: Yue-Lin
authors.initials: YL
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Shen
authors.forename: Ke-Jia
authors.initials: KJ
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Yun-Qiu
authors.initials: YQ
authors.lastname: Yu
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D016410:Lymphoma, T-Cell, Cutaneous / Q000235:genetics* / Q000378:metabolism*; D055442:Metabolome / Q000235:genetics*; D055432:Metabolomics / Q000379:methods; D025341:Principal Component Analysis / Q000379:methods; D011446:Prospective Studies; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D015415:Biomarkers