title: Assessment of changes in the BRCA2 and P53 genes in breast invasive ductal carcinoma in northeast Brazil.
abstract: BACKGROUND
  BRCA protein interacts with at least 13 different proteins that have been implicated with cancer susceptibility and loss of BRCA function is correlated to sensitivity to DNA crosslinking agents in preclinical models.
  
  
  RESULTS
  BRCA2 methylation frequency was 44%, p53 Pro22 allele frequency was 32% and heterozygous frequency of Arg/Pro72 genotype was 60% which could be associated as risk factor for metastasis (p = 0.046 OR = 4.190). Regarding to polymorphism of codon 249 the frequency of Arg249 allele presented 82% which was considered not statistically significant.
  
  
  CONCLUSIONS
  There was not statistical significance to BRCA2 promoter methylation with any parameters chosen. However, our findings suggest that patients who present heterozygous genotype at codon 72 of p53 gene may have a major susceptibility to any type of metastasis and this could serve as potential auxiliary biomarker for poor prognosis.
authors.forename: Eduardo Avf
authors.initials: EA
authors.lastname: Ramalho
authors.forename: João Lq
authors.initials: JL
authors.lastname: Silva-Filho
authors.forename: Marina Fs
authors.initials: MF
authors.lastname: Cartaxo
authors.forename: Carmelita Bl
authors.initials: CB
authors.lastname: Cavalcanti
authors.forename: Moacyr Jbm
authors.initials: MJ
authors.lastname: Rêgo
authors.forename: Maria Bm
authors.initials: MB
authors.lastname: Oliveira
authors.forename: Eduardo Ic
authors.initials: EI
authors.lastname: Beltrão
mesh_terms: D000483:Alleles; D001938:Brazil; D001943:Breast Neoplasms / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D024522:Genes, BRCA2; D016158:Genes, p53; D005838:Genotype; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D012307:Risk Factors; D013223:Statistics as Topic