title: Rosmarinic acid improves function and in vitro fertilising ability of boar sperm after cryopreservation.
abstract: During cryopreservation, oxidative stress exerts physical and chemical changes on sperm functionality. In the present study we investigated the antioxidant effect of rosmarinic acid (RA) on quality and fertilising ability of frozen-thawed boar spermatozoa. Ejaculates collected from mature boar were cryopreserved in lactose-egg yolk buffer supplemented with different concentrations of RA (0 μM, 26.25 μM, 52.5 μM and 105 μM). Motion parameters, acrosome and plasma membrane integrity, lipoperoxidation levels, DNA oxidative damage (8-hydroxy-2-deoxyguanosine base lesion) and in vitro fertilisation ability were evaluated. Total and progressive motility were significantly higher in experimental extenders with RA than in the control (P<0.05) at 0 and 120 min post-thawing. The plasma and acrosomal membrane integrity were improved by supplementation with 105 μMRA (P<0.05). Negative correlation between RA and malondialdehyde (MDA) concentration were determined (P<0.05). After thawing, the percentage of spermatozoa with oxidised DNA did not differ between extenders, however, at 120 and 240 min post-thawing, the samples supplemented with 105 μMRA showed the lowest DNA oxidation rate (P<0.05). The penetration rate was significantly higher on spermatozoa cryopreserved with 105 μMRA (P<0.05). The results suggest that RA provides a protection for boar spermatozoa against oxidative stress during cryopreservation by their antioxidant properties.
authors.affiliation: Department of Animal Pathology, Reproduction and Obstetrics Area, Veterinary Faculty, Universidad de Zaragoza, Spain. Electronic address: vicluno@unizar.es.
authors.forename: Victoria
authors.initials: V
authors.lastname: Luño
authors.affiliation: Department of Animal Pathology, Reproduction and Obstetrics Area, Veterinary Faculty, Universidad de Zaragoza, Spain.
authors.forename: Lydia
authors.initials: L
authors.lastname: Gil
authors.affiliation: Department of Animal Pathology, Reproduction and Obstetrics Area, Veterinary Faculty, Universidad de Zaragoza, Spain.
authors.forename: Maite
authors.initials: M
authors.lastname: Olaciregui
authors.affiliation: Department of Animal Pathology, Reproduction and Obstetrics Area, Veterinary Faculty, Universidad de Zaragoza, Spain.
authors.forename: Noelia
authors.initials: N
authors.lastname: González
authors.affiliation: Department of Animal Pathology, Reproduction and Obstetrics Area, Veterinary Faculty, Universidad de Zaragoza, Spain.
authors.forename: Rodrigo Alberto
authors.initials: RA
authors.lastname: Jerez
authors.affiliation: Department of Animal Pathology, Infectious Diseases Area, Veterinary Faculty, Universidad de Zaragoza, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: de Blas
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002934:Cinnamates / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004249:DNA Damage / Q000235:genetics; D053630:Depsides / Q000494:pharmacology*; D004527:Egg Proteins / Q000494:pharmacology; D004530:Egg Yolk; D005307:Fertilization in Vitro / Q000379:methods; D005615:Freezing / Q000009:adverse effects; D007785:Lactose / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects; D034421:Sus scrofa; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D002934:Cinnamates; D003451:Cryoprotective Agents; D053630:Depsides; D004527:Egg Proteins; D007785:Lactose; D008315:Malondialdehyde; D000097123:Rosmarinic Acid
keywords: Antioxidant; Boar sperm; Cryopreservation; Rosmarinic acid