title: Synthesis and characterization of silver nanoparticles using crystal compound of sodium para-hydroxybenzoate tetrahydrate isolated from Vitex negundo. L leaves and its apoptotic effect on human colon cancer cell lines.
abstract: Metallic nanoparticles are major concern, particularly silver nanoparticles (AgNPs) are used in various applications. In the present investigation, we report a novel strategy with biological approach for synthesis of AgNPs using sodium para-hydroxybenzoate tetrahydrate (SPHT) isolated from Vitex negundo leaves. The synthesized SPHT-AgNPs were characterized by UV-vis spectroscopy, high resolution transmission electron microscopy (HRTEM) with selected area electron diffraction (SAED) pattern, field emission scanning electron microscopy (FESEM) with energy-dispersive X-ray spectroscopy (EDX), zeta potential and Fourier transform infrared spectroscopy (FT-IR) analysis. The various pH and temperature were evaluated to find their stability effects on SPHT-AgNPs synthesis peak at 430 nm. The size of SPHT-AgNPs were ranging from 26 to 39 nm and were spherical in shape. The hydroxyl and carboxylic functional groups from bio-reducing mediators of SPHT have a stronger ability towards synthesis of AgNPs, which was confirmed using FT-IR spectrum. In addition, anticancer activity were determined by MTT assay, Annexin V-FITC/PI and cell cycle analysis.
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: Prabhu
authors.initials: P
authors.lastname: Durai
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India. Electronic address: carulvasu@gmail.com.
authors.forename: Arulvasu
authors.initials: A
authors.lastname: Chinnasamy
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: Babu
authors.initials: B
authors.lastname: Gajendran
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: Manikandan
authors.initials: M
authors.lastname: Ramar
authors.affiliation: Department of Bioinformatics, Alagappa University, Karaikudi 630 003, Tamilnadu, India.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Pappu
authors.affiliation: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600119, Tamilnadu, India.
authors.forename: Govindaraju
authors.initials: G
authors.lastname: Kasivelu
authors.affiliation: Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore 632 115, Tamilnadu, India.
authors.forename: Ashokkumar
authors.initials: A
authors.lastname: Thirunavukkarasu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D019073:HT29 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010226:Parabens / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013499:Surface Properties; D020950:Vitex / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D009942:Organometallic Compounds; D010226:Parabens; D010936:Plant Extracts; D012834:Silver; C038193:4-hydroxybenzoic acid
keywords: Apoptosis; Cytotoxicity; Nano biotechnology; Sodium para-hydroxybenzoate tetrahydrate; Vitex negundo