title: [Determination of amino acids in Galli Gigerii endothelium corneum by HPLC with pre-column derivatization].
abstract: Hydrolytic amino acids were extracted by acid hydrolysis method, then derivatized with phenyl isothiocyanate (PITC). And the samples were analysed by HPLC on an Ultimate Prime C18 (4.6 mm x 250 mm, 5 microm) column with gradient elution of 0.1 mol x L(-1) sodium acetate buffer solution (adjusted to pH 6. 5)-acetonitrile (93:7) (A) and acetonitrile-water (8:2) (B) at a flow rate of 1.0 mL x min(-1). Column temperature was 40 degrees C and the detected wavelength was 254 nm. Amino acids derivative solution remained stable in 36 hours. The response was linear for 16 amino acids with a correlation coefficient r > 0.999 5. The average recoveries were 98.01% -101.8%. The method is reliable with good accuracy and repeatability, which is useful for the determination of amino acids in Galli Gigerii Endothelium Corneum.
authors.forename: Kun
authors.initials: K
authors.lastname: Liang
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.forename: Ji
authors.initials: J
authors.lastname: Shi
authors.forename: Hong
authors.initials: H
authors.lastname: Hu
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Xiu
authors.forename: Tian-Zhu
authors.initials: TZ
authors.lastname: Jia
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004727:Endothelium / Q000737:chemistry*; D005895:Gizzard, Avian / Q000737:chemistry*
chemical_list: D000596:Amino Acids