title: Identification of 23 novel conserved microRNAs in three rice cultivars.
abstract: Plant microRNAs (miRNAs) play important roles as modulators of gene expression at the post-transcriptional level. Previous studies have shown that high-throughput sequencing is a powerful tool for the identification of miRNAs, and it is believed that many more miRNAs remain to be discovered. Here, we found 23 novel conserved miRNAs from three rice cultivars by high-throughput sequencing and further identified these through subsequent cloning and quantitative real-time polymerase chain reaction (qPCR). Eight of these novel miRNAs were detected with significant signals in the three rice cultivars by northern blotting assays. The quantitative analysis of their expression profiles showed that most of these miRNAs were perfectly or imperfectly negatively correlated with their target genes, which suggests that these miRNAs may play important roles during rice development. This is the first genome-wide investigation of miRNAs from different rice cultivars, and the data obtained expand the known rice miRNA inventory and provide further information about the regulatory roles played by miRNAs in rice development.
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address: nather2008@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address: zhhengmu@tsinghua.org.cn.
authors.forename: Heng-Mu
authors.initials: HM
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China. Electronic address: lxy890101@hotmail.com.
authors.forename: Xiao-Ya
authors.initials: XY
authors.lastname: Liu
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address: lijing82925@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address: lvmingfang309@163.com.
authors.forename: Ming-Fang
authors.initials: MF
authors.lastname: Lv
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China. Electronic address: lipeipei_0323@163.com.
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Li
authors.affiliation: College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128, China. Electronic address: liangying_99@yahoo.com.
authors.forename: Liang-Ying
authors.initials: LY
authors.lastname: Dai
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address: jpchen2001@yahoo.com.cn.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D012275:Oryza / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D018749:RNA, Plant / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000378:metabolism; D036226:Seedlings / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D016601:RNA-Binding Proteins
keywords: High-throughput RNA sequencing; MicroRNAs (miRNAs); Quantitative real-time polymerase chain reaction (qPCR); Rice (Oryza sativa)