title: Oral delivery of probiotic expressing M cell homing peptide conjugated BmpB vaccine encapsulated into alginate/chitosan/alginate microcapsules.
abstract: Oral administration of live probiotics as antigen delivery vectors is a promising approach in vaccine development. However, the low survival of probiotics in the gastrointestinal tract limits this approach. Therefore, the aim of this study was the encapsulation of probiotic expressing vaccine into alginate/chitosan/alginate (ACA) microcapsules (MCs) for efficient oral vaccine delivery. Here, recombinant Lactobacillus plantarum 25 (LP25) expressing M cell homing peptide fused BmpB protein was used as a model probiotic. The viability of LP25 in ACA MCs was more than 65% in simulated gastric fluid (SGF, pH 2.0) and 75% in simulated small intestinal fluid (SIF, pH 7.2) up to 2h. Encapsulated LP25 was completely released from ACA MCs in SIF within 12h. When stored at room temperature (RT) or 4°C, the viability of LP25 in ACA MCs was higher than free LP25. Interestingly, the viability of LP25 in ACA MCs at 4°C for 5weeks was above 58%, whereas viability of free LP25 stored at RT up to 5weeks was zero. After 4weeks from the first immunization, LP25-M-BmpB-loaded ACA MCs induced a stronger BmpB-specific IgG and IgA production in mice. Collectively, these findings suggest that encapsulation of probiotic by ACA MCs is a promising delivery system for oral administration of probiotic expressing vaccine.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Bijay
authors.initials: B
authors.lastname: Singh
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sushila
authors.initials: S
authors.lastname: Maharjan
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hui-Shan
authors.initials: HS
authors.lastname: Li
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sang-Kee
authors.initials: SK
authors.lastname: Kang
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jin-Duck
authors.initials: JD
authors.lastname: Bok
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea. Electronic address: chocs@snu.ac.kr.
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea. Electronic address: cyjcow@snu.ac.kr.
authors.forename: Yun-Jaie
authors.initials: YJ
authors.lastname: Choi
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage* / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D002214:Capsules; D002460:Cell Line; D048271:Chitosan / Q000008:administration & dosage*; D016503:Drug Delivery Systems / Q000379:methods; D005136:Eye Proteins / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D020723:Glucuronic Acid / Q000008:administration & dosage; D006603:Hexuronic Acids / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000276:immunology; D019936:Probiotics / Q000008:administration & dosage*; D018074:Vaccines, Conjugate
chemical_list: D000464:Alginates; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; C501736:BmpB protein, Borrelia burgdorferi; D002214:Capsules; D005136:Eye Proteins; D006603:Hexuronic Acids; D010446:Peptide Fragments; D018074:Vaccines, Conjugate; C052615:peptide M, retinal S antigen; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Cytokine induction; Lactobacillus plantarum 25; M cell homing peptide; Microencapsulation; Oral delivery; Probiotic expressing vaccine