title: Purification and characterisation of plantaricin ZJ008, a novel bacteriocin against Staphylococcus spp. from Lactobacillus plantarum ZJ008.
abstract: A novel bacteriocin, plantaricin ZJ008 produced by Lactobacillus plantarum ZJ008 isolated from fresh milk, was purified by XAD 2, cation exchange chromatograph, gel chromatograph, and RP-HPLC. Mass spectrometry based on MALDI-TOF indicated that the bacteriocin had a molecular of 1334.77 Da. Only five of twenty amino acids could be identified by Edman degradation. This bacteriocin was highly thermostable (121°C, 30 min) and exhibited narrow pH stability (pH 4.0-5.0). It was sensitive to α-Chymotrypsin, trypsin, papain, and pepsin. However it still had 80% of activity after treatment by proteinase K. The action mode of this peptide functioned as bactericidal, but it did not lead to lysis of cells. This bacteriocin exhibited broad-spectrum antimicrobial activity against tested Gram-positive and Gram-negative bacteria, especially Staphylococcus spp. These results suggested that this bacteriocin appears potentially very useful to control and inhibit Staphylococcus spp. in the food industry.
authors.affiliation: Zhejiang Gongshang University, Key Laboratory for Food Microbial Technology of Zhejiang Province, Hangzhou, Zhejiang Province, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Zhejiang Gongshang University, Key Laboratory for Food Microbial Technology of Zhejiang Province, Hangzhou, Zhejiang Province, China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Zhejiang Gongshang University, Key Laboratory for Food Microbial Technology of Zhejiang Province, Hangzhou, Zhejiang Province, China.
authors.forename: Yalian
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Zhejiang Gongshang University, Key Laboratory for Food Microbial Technology of Zhejiang Province, Hangzhou, Zhejiang Province, China. Electronic address: guqing2002@hotmail.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Gu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000378:metabolism*; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins
keywords: Bacteriocin; Potassium efflux; Staphylococcus aureus