title: Optimized cell transplantation using adult rag2 mutant zebrafish.
abstract: Cell transplantation into adult zebrafish has lagged behind mouse models owing to the lack of immunocompromised strains. Here we have created rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft muscle, blood stem cells and various cancers. rag2(E450fs) mutant zebrafish are the first immunocompromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer.
authors.affiliation: 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. [5].
authors.forename: Qin
authors.initials: Q
authors.lastname: Tang
authors.affiliation: 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. [5].
authors.forename: Nouran S
authors.initials: NS
authors.lastname: Abdelfattah
authors.affiliation: 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. [5].
authors.forename: Jessica S
authors.initials: JS
authors.lastname: Blackburn
authors.affiliation: 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Moore
authors.affiliation: 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Martinez
authors.affiliation: 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
authors.forename: Finola E
authors.initials: FE
authors.lastname: Moore
authors.affiliation: 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
authors.forename: Riadh
authors.initials: R
authors.lastname: Lobbardi
authors.affiliation: 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
authors.forename: Inês M
authors.initials: IM
authors.lastname: Tenente
authors.affiliation: 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
authors.forename: Myron S
authors.initials: MS
authors.lastname: Ignatius
authors.affiliation: Izaak Walton Killam Health Centre, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: Jason N
authors.initials: JN
authors.lastname: Berman
authors.affiliation: Izaak Walton Killam Health Centre, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Liwski
authors.affiliation: 1] Department of Surgery, Weill Cornell Medical College, New York, New York, USA. [2] Department of Medicine, Weill Cornell Medical College, New York, New York, USA.
authors.forename: Yariv
authors.initials: Y
authors.lastname: Houvras
authors.affiliation: 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. [5].
authors.forename: David M
authors.initials: DM
authors.lastname: Langenau
mesh_terms: D000368:Aged; D000818:Animals; D017690:Cell Transplantation; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D009154:Mutation; D015027:Zebrafish / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse