title: Multi-spectral characterization & effect of metal ions on the binding of bovine serum albumin upon interaction with a lincosamide antibiotic drug, clindamycin phosphate.
abstract: The interaction of clindamycin phosphate (CP) with bovine serum albumin (BSA) is studied by using fluorescence spectra, UV-visible absorption, synchronous fluorescence spectra (SFS), CD, 3D fluorescence spectra and lifetime measurements under simulated physiological conditions. CP effectively quenched intrinsic fluorescence of BSA. The binding constants KA values are 2.540×10(5), 4.960×10(5), 7.207×10(5) L mol(-1), the number of binding sites n and corresponding thermodynamic parameters ΔG(o), ΔH(o) and ΔS(o) between CP and BSA were calculated at different temperatures. The interaction between CP and BSA occurs through dynamic quenching and the effect of CP on the conformation of BSA was also analyzed using SFS. The average binding distance r between the donor (BSA) and acceptor (CP) was determined based on Förster's theory. The results of fluorescence spectra, UV-vis absorption spectra and SFS show that the secondary structure of the protein has been changed in the presence of CP.
authors.affiliation: P.G. Department of Studies in Chemistry, Karnatak University, Dharwad 580003, India.
authors.forename: Manjunath D
authors.initials: MD
authors.lastname: Meti
authors.affiliation: P.G. Department of Studies in Chemistry, Karnatak University, Dharwad 580003, India.
authors.forename: Kirthi S
authors.initials: KS
authors.lastname: Byadagi
authors.affiliation: P.G. Department of Studies in Chemistry, Karnatak University, Dharwad 580003, India.
authors.forename: Sharanappa T
authors.initials: ST
authors.lastname: Nandibewoor
authors.affiliation: P.G. Department of Studies in Chemistry, Karnatak University, Dharwad 580003, India. Electronic address: schimatadar@gmail.com.
authors.forename: Shivamurti A
authors.initials: SA
authors.lastname: Chimatadar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D002417:Cattle; D002981:Clindamycin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D004735:Energy Transfer; D007477:Ions / Q000737:chemistry; D007700:Kinetics; D055231:Lincosamides / Q000737:chemistry / Q000378:metabolism; D008670:Metals / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D000900:Anti-Bacterial Agents; D007477:Ions; D055231:Lincosamides; D008670:Metals; D012710:Serum Albumin, Bovine; D002981:Clindamycin; C007084:clindamycin phosphate
keywords: 3D spectra; Bovine serum albumin; Clindamycin phosphate; Lifetime measurements; Quenching