title: Separation of enantiomers on chiral stationary phase based on chicken α₁-acid glycoprotein: effect of silica particle diameters on column performance.
abstract: The effects of silica particle diameters on performances of chicken α₁-acid glycoprotein (c-AGP)-immobilized silica particle columns were investigated. c-AGP was immobilized onto aminopropyl silica particles, whose nominal particle diameters were 5, 3 and 2.1 μm, activated with N,N'-disuccinimidyl carbonate. The retention factor (k), enantioseparation factor (α), resolution (Rs) and height equivalent to a theoretical plate (H) of solutes on three c-AGP columns were evaluated using a mixture of phosphate buffer and organic modifier as a mobile phase in LC. There were not so much differences in their k and α values among three c-AGP columns, while their Rs values were in the order of 2.1 μm>3 μm>5 μm silica particles and their H values were in the reversed order. Since three c-AGP columns gave almost the same enantioseparation factors for solutes, their highest Rs and lowest H values on a c-AGP-immobilized column prepared with 2.1-μm silica particles came from its highest column efficiency among there c-AGP columns. These results suggest that 2.1-μm silica particles could be useful for the preparation of c-AGP- or protein-based CSPs.
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, 11-68, Koshien Kyuban-cho, Nishinomiya 663-8179, Japan.
authors.forename: Hisami
authors.initials: H
authors.lastname: Matsunaga
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, 11-68, Koshien Kyuban-cho, Nishinomiya 663-8179, Japan. Electronic address: haginaka@mukogawa-u.ac.jp.
authors.forename: Jun
authors.initials: J
authors.lastname: Haginaka
mesh_terms: D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid; D009961:Orosomucoid / Q000737:chemistry*; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D009961:Orosomucoid; D012822:Silicon Dioxide
keywords: Chicken α(1)-acid glycoprotein; Chiral stationary phase; Enantiomer separation; Ibuprofen; Propranolol