title: [Effects of total flavones of Elsholtzia splendens in isolated ischemia/reperfusion rat hearts].
abstract: OBJECTIVE
  To investigate the influence of total flavonoids of Elsholtzia splendens (TFES) on isolated ischemia/reperfusion rat hearts and its underlying mechanisms.
  
  
  METHODS
  Hearts isolated from male SD rats were perfused on the Langendorff apparatus and subjected to global ischemia for 30 min followed by 120 min of reperfusion. The cardiac infarct size was measured by TTC staining. Hemodynamic parameters and the level of lactate dehydrogenase (LDH) in the coronary effluent were measured. Absorbance at 520 nm was determined in isolated cardiac mitochondria exposed to 200 micromol/L CaCl2 to detect the opening of the mitochondrial permeability transition pore.
  
  
  RESULTS
  Pretreatment with TFES (1, 10, 100 microg/ml) for 5 min decreased infarct size and LDH release and improved the recovery of the left ventricular developed pressure. In mitochondria, the decrease of absorbance at 520 nm evoked by CaCl2 was greatly inhibited by TFES.
  
  
  CONCLUSIONS
  TFES prevents myocardial ischemia/reperfusion injury, and this cardioprotective effect is probably via inhibiting mitochondrial permeability transition pore opening.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Ling-Bo
authors.initials: LB
authors.lastname: Qian
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Ye
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Wang
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology; D004195:Disease Models, Animal; D047309:Flavones / Q000494:pharmacology*; D066298:In Vitro Techniques; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects; D033681:Mitochondrial Membrane Transport Proteins / Q000187:drug effects; D000083162:Mitochondrial Permeability Transition Pore; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002316:Cardiotonic Agents; D047309:Flavones; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore