title: Tolerating DNA damage during eukaryotic chromosome replication.
abstract: In eukaryotes, the evolutionarily conserved RAD6/RAD18 pathway of DNA damage tolerance overcomes unrepaired DNA lesions that interfere with the progression of replication forks, helping to ensure the completion of chromosome replication and the maintenance of genome stability in every cell cycle. This pathway uses two different strategies for damage bypass: translesion DNA synthesis, which is carried out by specialized polymerases that can replicate across the lesions, and DNA damage avoidance, a process that relies on a switch to an undamaged-DNA template for synthesis past the lesion. In this review, we summarise the current knowledge on DNA damage tolerance mechanisms mediated by RAD6/RAD18 that are used by eukaryotic cells to cope with DNA lesions during chromosome replication.
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC/UAM), Cantoblanco, 28049 Madrid, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Saugar
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC/UAM), Cantoblanco, 28049 Madrid, Spain.
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Ortiz-Bazán
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC/UAM), Cantoblanco, 28049 Madrid, Spain. Electronic address: jatercero@cbm.csic.es.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Tercero
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D005057:Eukaryotic Cells / Q000378:metabolism*; D006801:Humans
keywords: Chromosome replication; DNA damage tolerance; Genome stability