title: The effects of different culture media on human corneal endothelial cells.
abstract: OBJECTIVE
  To investigate the most appropriate media condition for the proliferation and functional maintenance of human corneal endothelial cells (HCECs).
  
  
  METHODS
  We cultured HCECs in traditional media (medium A or D) and in stem cell media (medium E or N). The morphology of the cells was evaluated by inverted microscopy. Collagen, type VIII, alpha 2 and sodium-potassium adenosine triphosphatase (Na(+)-K(+) ATPase) expression were analyzed as differentiation markers. Octamer-binding transcription factor 3/4, glial fibrillary acidic protein, nestin and β-catenin expression were evaluated as stem cell associated proteins. The cell proliferation rate was evaluated with a cell counting kit-8 assay. Wound healing assays were also performed. The transendothelial electrical potential difference (TEPD) value was used to estimate the endothelial cell permeability in vitro.
  
  
  RESULTS
  The proliferation and morphology analyses demonstrated that there were significant differences between the media. The expression of differentiation markers and stem cell-associated proteins was different between the media. Medium D resulted in higher proliferation rates compared with the other media, while still maintaining the differentiation potential and surface marker expression profile characteristic of HCECs. Compared with other media, TEPD was higher in medium N.
  
  
  CONCLUSIONS
  Culture medium D was superior to the other media with regard to the expression of stem cell-associated proteins, proliferation, and cell migration. However, medium N was more appropriate than the other three media with regard to maintaining the proper cell shape and function.
authors.affiliation: Department of Ophthalmology, Hallym University College of Medicine, Seoul, Republic of Korea.
authors.forename: Eunbi
authors.initials: E
authors.lastname: Kim
authors.affiliation: Department of Ophthalmology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, Korea.
authors.forename: Jin Joo
authors.initials: JJ
authors.lastname: Kim
authors.affiliation: Department of Ophthalmology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, Korea Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Joon Young
authors.initials: JY
authors.lastname: Hyon
authors.affiliation: Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Eui-Sang
authors.initials: ES
authors.lastname: Chung
authors.affiliation: Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Chung
authors.affiliation: Department of Ophthalmology, Hallym University College of Medicine, Seoul, Republic of Korea.
authors.forename: Kayoung
authors.initials: K
authors.lastname: Yi
authors.affiliation: Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Won Ryang
authors.initials: WR
authors.lastname: Wee
authors.affiliation: Department of Ophthalmology, Hallym University College of Medicine, Seoul, Republic of Korea.
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Shin
mesh_terms: D015415:Biomarkers; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D055724:Electrophysiological Phenomena / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D004728:Endothelium, Corneal / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D015415:Biomarkers; D003470:Culture Media
keywords: COL8A2; GFAP; Na+-K+ ATPase; OCT3/4; human corneal endothelial cells; media; nestin