title: Superovulation alters embryonic poly(A)-binding protein (Epab) and poly(A)-binding protein, cytoplasmic 1 (Pabpc1) gene expression in mouse oocytes and early embryos.
abstract: Embryonic poly(A)-binding protein (EPAB) and poly(A)-binding protein, cytoplasmic 1 (PABPC1) play critical roles in translational regulation of stored maternal mRNAs required for proper oocyte maturation and early embryo development in mammals. Superovulation is a commonly used technique to obtain a great number of oocytes in the same developmental stages in assisted reproductive technology (ART) and in clinical or experimental animal studies. Previous studies have convincingly indicated that superovulation alone can cause impaired oocyte maturation, delayed embryo development, decreased implantation rate and increased postimplantation loss. Although how superovulation results in these disturbances has not been clearly addressed yet, putative changes in genes related to oocyte and early embryo development seem to be potential risk factors. Thus, the aim of the present study was to determine the effect of superovulation on Epab and Pabpc1 gene expression. To this end, low- (5IU) and high-dose (10IU) pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotrophin (hCG) were administered to female mice to induce superovulation, with naturally cycling female mice serving as controls. Epab and Pabpc1 gene expression in germinal vesicle (GV) stage oocytes, MII oocytes and 1- and 2-cell embryos collected from each group were quantified using quantitative reverse transcription-polymerase chain reaction. Superovulation with low or high doses of gonadotropins significantly altered Epab and Pabpc1 mRNA levels in GV oocytes, MII oocytes and 1- and 2-cell embryos compared with their respective controls (P<0.05). These changes most likely lead to variations in expression of EPAB- and PABPC1-regulated genes, which may adversely influence the quality of oocytes and early embryos retrieved using superovulation.
authors.affiliation: Department of Histology and Embryology, Akdeniz University, School of Medicine, Campus, 07070, Antalya, Turkey.
authors.forename: Saffet
authors.initials: S
authors.lastname: Ozturk
authors.affiliation: Department of Histology and Embryology, Istanbul Bilim University, School of Medicine, 34394, Sisli, Istanbul, Turkey.
authors.forename: Aylin
authors.initials: A
authors.lastname: Yaba-Ucar
authors.affiliation: Department of Histology and Embryology, Akdeniz University, School of Medicine, Campus, 07070, Antalya, Turkey.
authors.forename: Berna
authors.initials: B
authors.lastname: Sozen
authors.affiliation: Department of Medical Microbiology, Akdeniz University, School of Medicine, Campus, 07070, Antalya, Turkey.
authors.forename: Derya
authors.initials: D
authors.lastname: Mutlu
authors.affiliation: Department of Histology and Embryology, Akdeniz University, School of Medicine, Campus, 07070, Antalya, Turkey.
authors.forename: Necdet
authors.initials: N
authors.lastname: Demir
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D046149:Embryo Culture Techniques; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D008807:Mice, Inbred BALB C; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism; D010062:Ovulation Induction / Q000379:methods*; D039122:Poly(A)-Binding Protein I / Q000235:genetics / Q000378:metabolism*; D039102:Poly(A)-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013480:Superovulation / Q000187:drug effects*; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin; C575837:EPAB protein, mouse; D005300:Fertility Agents, Female; D006064:Gonadotropins, Equine; D039122:Poly(A)-Binding Protein I; D039102:Poly(A)-Binding Proteins