title: Downregulation of transketolase activity is related to inhibition of hippocampal progenitor cell proliferation induced by thiamine deficiency.
abstract: In animal experiments, hippocampal neurogenesis and the activity of thiamine-dependent transketolase decrease markedly under conditions of thiamine deficiency. To further investigate the effect of thiamine deficiency on the proliferation of hippocampal progenitor cells (HPCs) and the potential mechanisms involved in this effect, we cultured HPCs in vitro in the absence of thiamine and found that proliferation and transketolase activity were both significantly repressed. Furthermore, specific inhibition of transketolase activity by oxythiamine strongly inhibited HPC proliferation in a dose-dependent manner. However, thiamine deficiency itself inhibited the proliferation to a greater degree than did oxythiamine. Taken together, our results suggest that modulation of transketolase activity might be one of the mechanisms by which thiamine regulates the proliferation of hippocampal progenitor cells.
authors.affiliation: Department of Geriatrics, Shanghai First People's Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Geriatrics, Shanghai First People's Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, China.
authors.forename: Yiying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Geriatrics, Shanghai First People's Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, China.
authors.forename: Haolu
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Geriatrics, Shanghai First People's Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of Geriatrics, Shanghai First People's Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, China.
authors.forename: Min
authors.initials: M
authors.lastname: Ning
authors.affiliation: Department of Geriatrics, Shanghai First People's Hospital Affiliated Shanghai Jiaotong University, 100 Haining Road, Shanghai 200080, China.
authors.forename: Xiushi
authors.initials: X
authors.lastname: Ni
authors.affiliation: Department of Neurology, Zhongshan Hospital Affiliated Fudan University, 180 Fenglin Road, Shanghai 200032, China.
authors.forename: Chunjiu
authors.initials: C
authors.lastname: Zhong
mesh_terms: D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D049109:Cell Proliferation; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic; D006624:Hippocampus / Q000201:enzymology* / Q000473:pathology; D058953:Neural Stem Cells / Q000201:enzymology* / Q000473:pathology; D010119:Oxythiamine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013832:Thiamine Deficiency / Q000201:enzymology* / Q000473:pathology; D014174:Transketolase / Q000096:biosynthesis*
chemical_list: D000963:Antimetabolites; D010119:Oxythiamine; D014174:Transketolase