title: Dimeric guaianolides from Artemisia absinthium.
abstract: Five dimeric guaianolides, absinthins A-E, and seven known dimeric guaianolides were isolated from Artemisia absinthium. Their structures were elucidated based on 1D- and 2D-NMR experiments, including (1)H NMR, (13)C NMR, DEPT, (1)H-(1)H COSY, HSQC, HMBC, and NOESY, and through HRESIMS data analysis. The absolute configuration of the known compound, anabsinthin, was determined by X-ray crystallographic analysis. The isolated compounds were tested to assess their inhibitory activities on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV-2 cells; absinthin C and isoanabsinthin exhibited significant inhibitory effects with IC50 values of 1.52 and 1.98μM, respectively.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, People's Republic of China.
authors.forename: Ablajan
authors.initials: A
authors.lastname: Turak
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Traditional Chinese Medicine, Beijing 100029, People's Republic of China.
authors.forename: She-Po
authors.initials: SP
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, People's Republic of China. Electronic address: yongjiang@bjmu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, People's Republic of China. Electronic address: pengfeitu@vip.163.com.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Tu
mesh_terms: D031171:Artemisia absinthium / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification; D045789:Sesquiterpenes, Guaiane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D012717:Sesquiterpenes; D045789:Sesquiterpenes, Guaiane; C499475:absinthin; D009569:Nitric Oxide; C459142:anabsinthin
keywords: Artemisia absinthium; Asteraceae; BV-2 cells; Dimeric guaianolides; Inhibition of NO production; Single-crystal X-ray analysis; Wormwood