title: [Effect of Sijunzi decoction on the proliferation of side population cells of human gastric cancer cell line].
abstract: OBJECTIVE
  To observe the proliferation changes of the side population of gastric cancer cell line SGC-7901 cells (SP), the non-side population (NSP) cells, and unsorted cells (Total) after intervened by Sijunzi Decoction (SD) containing serum.
  
  
  METHODS
  Sixteen pure bred New Zealand rabbits were equally divided into the normal control group, the low dose SD group (at the daily dose of 7 mL/kg), the middle dose SD group (at the daily dose of 14 mL/kg), and the high dose SD group (at the daily dose of 28 mL/kg) according to the random digit table. Rabbits' serum was extracted after equal volume of corresponding medication was given by gastrogavage twice daily for 2 consecutive weeks. The drug serum was identified using high performance liquid chromatography. SP cells of SGC-7901 were detected using flow cytometry, SP and NSP cells were screened. The proliferation curve of SP, NSP, and Total cells were detected with CCK-8 assay. Changes of their proliferation were also observed.
  
  
  RESULTS
  Ginsenoside Rg1, an effective ingredient in SD was detected in prepared drug serum. The proliferation of SGC-7901 SP cells was significantly higher than that of NSP cells and Total cells (P < 0.05). Drug serum on gastric cancer cell line SGC-7901 SP, NSP, and Total cells could inhibit their proliferation, but its inhibition on SP cells' proliferation was significantly lower than on NSP and Total cells (P < 0.05).
  
  
  CONCLUSIONS
  SD could significantly inhibit the proliferation of gastric cancer cell line SGC-7901 SP, NSP, and Total cells. But there exist obvious difference in the inhibition among the three groups.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Jian-guang
authors.initials: JG
authors.lastname: Jia
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Da-jun
authors.initials: DJ
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Xie
authors.forename: Li-yu
authors.initials: LY
authors.lastname: Qian
authors.forename: Li-gong
authors.initials: LG
authors.lastname: Zhang
authors.forename: Chen-xu
authors.initials: CX
authors.lastname: Guo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D011817:Rabbits; D058985:Side-Population Cells / Q000187:drug effects / Q000473:pathology*; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D004365:Drugs, Chinese Herbal