title: Molecular cloning and function analysis of insulin-like growth factor-binding protein 1a in blunt snout bream (Megalobrama amblycephala).
abstract: Insulin-like growth factor-binding protein 1 (IGFBP-1), a hypoxia-induced protein, is a member of the IGFBP family that regulates vertebrate growth and development. In this study, full-length IGFBP-1a cDNA was cloned from a hypoxia-sensitive Cyprinidae fish species, the blunt snout bream (Megalobrama amblycephala). IGFBP-1a was expressed in various organs of adult blunt snout bream, including strongly in the liver and weakly in the gonads. Under hypoxia, IGFBP-1a mRNA levels increased sharply in the skin, liver, kidney, spleen, intestine and heart tissues of juvenile blunt snout bream, but recovered to normal levels after 24-hour exposure to normal dissolved oxygen. In blunt snout bream embryos, IGFBP-1a mRNA was expressed at very low levels at both four and eight hours post-fertilization, and strongly at later stages. Embryonic growth and development rates decreased significantly in embryos injected with IGFBP-1a mRNA. The average body length of IGFBP-1a-overexpressed embryos was 82.4% of that of the control group, and somite numbers decreased to 85.2%. These findings suggest that hypoxia-induced IGFBP-1a may inhibit growth in this species under hypoxic conditions.
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Tian
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Xia-Yun
authors.initials: XY
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, China. smzou@shou.edu.cn.
authors.forename: Shu-Ming
authors.initials: SM
authors.lastname: Zou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000378:metabolism*; D005786:Gene Expression Regulation; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D018970:Insulin-Like Growth Factor Binding Protein 1
keywords: Hypoxia; IGFBP-1a; Megalobrama amblycephala; Overexpression