title: Determination of the degree of deacetylation of chitosan by capillary zone electrophoresis.
abstract: In this study we have developed a capillary zone electrophoresis (CZE) method for the determination of the degree of deacetylation (DDA) of chitosan. The electrophoretic mobilities of chitosans were measured by using the optimized CZE conditions. An internal standard, hexammine cobalt(III) chloride, was used to improve the precision of the electrophoretic mobility measurement. We have constructed a linear calibration curve between the CZE measured mobilities and the (1)H NMR determined DDAs ranging from 55.3% to 96.2%. Based on the established linear calibration equation and the CZE measured mobilities, we were able to obtain not only the average DDA values but also the DDA distribution profiles of the chitosan samples. Without prior sample treatment or purification, we have successfully employed the newly developed CZE method to characterize the reacetylation derivatives of chitosans and to detect the DDA variability in different lots of chitosan products.
authors.affiliation: Department of Chemistry, Tamkang University, 151 Yingchuan Road, Tamsui District, New Taipei City 25137, Taiwan. Electronic address: cwu@mail.tku.edu.tw.
authors.forename: Chunhung
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Tamkang University, 151 Yingchuan Road, Tamsui District, New Taipei City 25137, Taiwan.
authors.forename: Chen Yao
authors.initials: CY
authors.lastname: Kao
authors.affiliation: Department of Chemistry, Tamkang University, 151 Yingchuan Road, Tamsui District, New Taipei City 25137, Taiwan.
authors.forename: Shih-Ying
authors.initials: SY
authors.lastname: Tseng
authors.affiliation: Department of Chemistry, Tamkang University, 151 Yingchuan Road, Tamsui District, New Taipei City 25137, Taiwan.
authors.forename: Kuan Cheng
authors.initials: KC
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Tamkang University, 151 Yingchuan Road, Tamsui District, New Taipei City 25137, Taiwan.
authors.forename: Shih-Feng
authors.initials: SF
authors.lastname: Chen
mesh_terms: D000107:Acetylation; D048271:Chitosan / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods
chemical_list: D048271:Chitosan
keywords: Capillary zone electrophoresis; Chitosan; Degree of deacetylation; Distribution