title: Genotoxicity test of self-renovated ceramics in primary human peripheral lymphocytes.
abstract: Zirconia-based ceramics is widely used in dentistry. Different compositions of ceramics have different features. Our self-renovated ceramics become more machinable without scarifying its dental restoration properties after adjusting ratio of lanthanum phosphate (LaPO4)/yttrium oxide (Y2O3). In order to evaluate its safety, here, we tested its genotoxicity in primary human peripheral lymphocytes. The human lymphocytes cultured on three groups of different ratios of LaPO4/Y2O3 diphase ceramics for 6 days showed little effect of growth inhibition and similar effect of growth trend to the negative control. Furthermore, single-cell gel electrophoresis (comet assay) indicated that there was no significant difference of the value of tail moment between the tested ceramics and negative control, the IPS Empress II (P > 0.05). Our findings implicate that our self-renovated ceramics do not induce DNA damages in human peripheral lymphocytes and support their future clinic application.
authors.affiliation: Department of Stomatology, Shanghai Yangsi Hospital, Shanghai, 200126, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Hua
authors.forename: Huifang
authors.initials: H
authors.lastname: Zhu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhuang
authors.forename: Liping
authors.initials: L
authors.lastname: Chen
mesh_terms: D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009152:Mutagenicity Tests; D010710:Phosphates / Q000737:chemistry / Q000633:toxicity; D015019:Yttrium / Q000737:chemistry / Q000633:toxicity; D015040:Zirconium / Q000737:chemistry / Q000633:toxicity
chemical_list: D010710:Phosphates; C476779:lanthanum(III) phosphate; D015019:Yttrium; D015040:Zirconium; C028541:zirconium oxide; C091417:yttria