title: Sequence-based appraisal of the genes encoding neck and carbohydrate recognition domain of conglutinin in blackbuck (Antilope cervicapra) and goat (Capra hircus).
abstract: Conglutinin, a collagenous C-type lectin, acts as soluble pattern recognition receptor (PRR) in recognition of pathogens. In the present study, genes encoding neck and carbohydrate recognition domain (NCRD) of conglutinin in goat and blackbuck were amplified, cloned, and sequenced. The obtained 488 bp ORFs encoding NCRD were submitted to NCBI with accession numbers KC505182 and KC505183. Both nucleotide and predicted amino acid sequences were analysed with sequences of other ruminants retrieved from NCBI GenBank using DNAstar and Megalign5.2 software. Sequence analysis revealed maximum similarity of blackbuck sequence with wild ruminants like nilgai and buffalo, whereas goat sequence displayed maximum similarity with sheep sequence at both nucleotide and amino acid level. Phylogenetic analysis further indicated clear divergence of wild ruminants from the domestic ruminants in separate clusters. The predicted secondary structures of NCRD protein in goat and blackbuck using SWISSMODEL ProtParam online software were found to possess 6 beta-sheets and 3 alpha-helices which are identical to the result obtained in case of sheep, cattle, buffalo, and nilgai. However, quaternary structure in goat, sheep, and cattle was found to differ from that of buffalo, nilgai, and blackbuck, suggesting a probable variation in the efficiency of antimicrobial activity among wild and domestic ruminants.
authors.affiliation: CWL, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Sasmita
authors.initials: S
authors.lastname: Barik
authors.affiliation: CWL, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Chandra Mohan
authors.initials: CM
authors.lastname: Sidappa
authors.affiliation: CWL, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Mohini
authors.initials: M
authors.lastname: Saini
authors.affiliation: Department of Physiology and Biochemistry, Hassan Veterinary College, KVAFSU, Bidar, Karnataka 585401, India.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Doreswamy
authors.affiliation: CWL, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Asit
authors.initials: A
authors.lastname: Das
authors.affiliation: CWL, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Sharma
authors.affiliation: Animal Biotechnology Division, IVRI, Izatnagar, Uttar Pradesh 243 122, India.
authors.forename: Praveen K
authors.initials: PK
authors.lastname: Gupta
mesh_terms: D000818:Animals; D000866:Antelopes / Q000235:genetics*; D002020:Buffaloes / Q000235:genetics; D002417:Cattle; D037222:Collectins / Q000737:chemistry / Q000235:genetics*; D006041:Goats / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012712:Serum Globulins / Q000737:chemistry / Q000235:genetics*; D012756:Sheep / Q000235:genetics; D013045:Species Specificity
chemical_list: D037222:Collectins; D012712:Serum Globulins; C024014:conglutinin