title: Ventral prostate fibrosis in the Akita mouse is associated with macrophage and fibrocyte infiltration.
abstract: A higher incidence of lower urinary tract symptoms (LUTS) among diabetic men is unexplained. Recently, prostate inflammation and fibrosis have been implicated as major contributing factors to bladder outlet obstruction and LUTS. We characterized the inflammatory cell infiltrate and collagen content of the anterior, dorsal, and ventral lobes of 18-week-old DBA2J.Ins2-Akita mice (Akita) and age-matched control mice. We performed hematoxylin and eosin staining to score tissue injury and inflammation, picrosirius red staining to quantitate collagen content, and immunostaining to identify monocytes/macrophages and infiltrating fibrocytes. We observed significantly greater numbers of monocytes/macrophages and fibrocytes specifically in the ventral prostate of the Akita mice and found that this was associated with significant greater collagen content specifically in the ventral prostate of the Akita mice. These observations support the inference that diabetes elicits monocyte/macrophage infiltration and collagen accumulation in the prostate and suggest that further study of Akita mice may inform translational studies of diabetes in the genesis prostatic inflammation, prostatic fibrosis, and LUTS.
authors.affiliation: Department of Urology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53717, USA ; Cellular and Molecular Biology Program, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI 53706, USA.
authors.forename: Sanghee
authors.identifier: 0000-0002-3229-2533
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Urology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53717, USA.
authors.forename: Guang
authors.identifier: 0000-0003-1179-6529
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Urology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53717, USA.
authors.forename: William
authors.identifier: 0000-0002-6676-6135
authors.initials: W
authors.lastname: Mulligan
authors.affiliation: Department of Urology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53717, USA.
authors.forename: Jerry
authors.identifier: 0000-0002-0559-4931
authors.initials: J
authors.lastname: Gipp
authors.affiliation: Department of Urology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53717, USA ; Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI 53717, USA.
authors.forename: Wade
authors.identifier: 0000-0003-3803-2449
authors.initials: W
authors.lastname: Bushman
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry*; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D005260:Female; D005355:Fibrosis; D006416:Hematoxylin / Q000737:chemistry; D007249:Inflammation; D059411:Lower Urinary Tract Symptoms / Q000175:diagnosis / Q000473:pathology; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009000:Monocytes / Q000166:cytology*; D010641:Phenotype; D011467:Prostate / Q000473:pathology*; D011470:Prostatic Hyperplasia / Q000175:diagnosis / Q000473:pathology
chemical_list: D003094:Collagen; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin