title: Proteomics of rice and Cochliobolus miyabeanus fungal interaction: insight into proteins at intracellular and extracellular spaces.
abstract: Necrotrophic fungal pathogen Cochliobolus miyabeanus causes brown spot disease in rice leaves upon infection, resulting in critical rice yield loss. To better understand the rice-C. miyabeanus interaction, we employed proteomic approaches to establish differential proteomes of total and secreted proteins from the inoculated leaves. The 2DE approach after PEG-fractionation of total proteins coupled with MS (MALDI-TOF/TOF and nESI-LC-MS/MS) analyses led to identification of 49 unique proteins out of 63 differential spots. SDS-PAGE in combination with nESI-LC-MS/MS shotgun approach was applied to identify secreted proteins in the leaf apoplast upon infection and resulted in cataloging of 501 unique proteins, of which 470 and 31 proteins were secreted from rice and C. miyabeanus, respectively. Proteins mapped onto metabolic pathways implied their reprogramming upon infection. The enzymes involved in Calvin cycle and glycolysis decreased in their protein abundance, whereas enzymes in the TCA cycle, amino acids, and ethylene biosynthesis increased. Differential proteomes also generated distribution of identified proteins in the intracellular and extracellular spaces, providing a better insight into defense responses of proteins in rice against C. miyabeanus. Established proteome of the rice-C. miyabeanus interaction serves not only as a good resource for the scientific community but also highlights its significance from biological aspects.
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, South Korea.
authors.forename: Jin Yeong
authors.initials: JY
authors.lastname: Kim
authors.forename: Jingni
authors.initials: J
authors.lastname: Wu
authors.forename: Soon Jae
authors.initials: SJ
authors.lastname: Kwon
authors.forename: Haram
authors.initials: H
authors.lastname: Oh
authors.forename: So Eui
authors.initials: SE
authors.lastname: Lee
authors.forename: Sang Gon
authors.initials: SG
authors.lastname: Kim
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.forename: Ganesh Kumar
authors.initials: GK
authors.lastname: Agrawal
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Kang
authors.forename: Il-Pyung
authors.initials: IP
authors.lastname: Ahn
authors.forename: Beom-Gi
authors.initials: BG
authors.lastname: Kim
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D054884:Host-Pathogen Interactions; D012275:Oryza / Q000378:metabolism / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins
keywords: Cochliobolus miyabeanus; Disease; Plant proteomics; Rice; Shotgun proteomics; Two-dimensional gel electrophoresis