title: Proliferating pancreatic beta-cells upregulate ALDH.
abstract: High levels of aldehyde dehydrogenase (ALDH) activity have been regarded as a specific feature of progenitor cells and stem cells. Hence, as an indicator of ALDH activity, aldefluor fluorescence has been widely used for the identification and isolation of stem and progenitor cells. ALDH activity was recently detected in embryonic mouse pancreas, and specifically and exclusively in adult centroacinar and terminal duct cells, suggesting that these duct cells may harbor cells of endocrine and exocrine differentiation potential in the adult pancreas. Here, we report the presence of aldefluor+ beta-cells in a beta-cell proliferation model, partial pancreatectomy. The aldefluor+ beta-cells are essentially all positive for Ki-67 and expressed high levels of cell-cycle activators such as CyclinD1, CyclinD2, and CDK4, suggesting that they are mitotic cells. Our data thus reveal a potential change in ALDH activity of proliferating beta-cells, which provides a novel method for the isolation and analysis of proliferating beta-cells. Moreover, our data also suggest that aldefluor lineage-tracing is not a proper method for analyzing progenitor or stem activity in the adult pancreas.
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Nanhua University, No. 69 Chuanshan Road, Hengyang, 421001, Hunan Province, China.
authors.forename: Yinglan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Jiang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zeng
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Renxian
authors.initials: R
authors.lastname: Cao
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation; D005434:Flow Cytometry; D050417:Insulin-Secreting Cells / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation; D051379:Mice; D015854:Up-Regulation
chemical_list: D000444:Aldehyde Dehydrogenase