title: Effects of a novel archaeal tetraether-based colipid on the in vivo gene transfer activity of two cationic amphiphiles.
abstract: Gene therapy for treating inherited diseases like cystic fibrosis might be achieved using multimodular nonviral lipid-based systems. To date, most optimizations have concerned cationic lipids rather than colipids. In this study, an original archaeal tetraether derivative was used as a colipid in combination with one or the other of two monocationic amphiphiles. The liposomes obtained, termed archaeosomes, were characterized regarding lipid self-assembling properties, macroscopic/microscopic structures, DNA condensation/neutralization/relaxation abilities, and colloidal stability in the presence of serum. In addition, gene transfer experiments were conducted in mice with lipid/DNA complexes being administered via systemic or local delivery routes. Altogether, the results showed that the tetraether colipid can provide complexes with different in vivo transfection abilities depending on the lipid combination, the lipid/colipid molar ratio, and the administration route. This original colipid appears thus as an innovative modular platform endowed with properties possibly beneficial for fine-tuning of in vivo lipofection and other biomedical applications.
authors.affiliation: Unité INSERM 1078, SFR ScInBioS; Université de Bretagne Occidentale, Université Européenne de Bretagne , 46 rue Félix Le Dantec, CS51819, 29218 Brest Cedex 02, France.
authors.forename: Tony
authors.initials: T
authors.lastname: Le Gall
authors.forename: Julie
authors.initials: J
authors.lastname: Barbeau
authors.forename: Sylvain
authors.initials: S
authors.lastname: Barrier
authors.forename: Mathieu
authors.initials: M
authors.lastname: Berchel
authors.forename: Loïc
authors.initials: L
authors.lastname: Lemiègre
authors.forename: Jelena
authors.initials: J
authors.lastname: Jeftić
authors.forename: Cristelle
authors.initials: C
authors.lastname: Meriadec
authors.forename: Franck
authors.initials: F
authors.lastname: Artzner
authors.forename: Deborah R
authors.initials: DR
authors.lastname: Gill
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Hyde
authors.forename: Claude
authors.initials: C
authors.lastname: Férec
authors.forename: Pierre
authors.initials: P
authors.lastname: Lehn
authors.forename: Paul-Alain
authors.initials: PA
authors.lastname: Jaffrès
authors.forename: Thierry
authors.initials: T
authors.lastname: Benvegnu
authors.forename: Tristan
authors.initials: T
authors.lastname: Montier
mesh_terms: D000818:Animals; D001105:Archaea / Q000737:chemistry*; D002412:Cations / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry; D004987:Ethers / Q000737:chemistry*; D005260:Female; D018014:Gene Transfer Techniques; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D051379:Mice; D013501:Surface-Active Agents / Q000737:chemistry*; D014162:Transfection / Q000379:methods
chemical_list: D002412:Cations; D004987:Ethers; D008055:Lipids; D008081:Liposomes; D013501:Surface-Active Agents; D004247:DNA