title: Effect of fluoride on sodium-proton exchanger activity, intracellular pH and calcium concentration in human non-stimulated platelets.
abstract: BACKGROUND
  Although the Na+/H+ exchanger (NHE) has been extensively investigated, studies on the effect of fluoride ions on the function of this protein are relatively few and controversial. The aim of this study was to analyze the effect of fluoride on the activity of the transmembrane NHE in human platelets.
  
  
  METHODS
  The study material consisted of platelets in the form of platelet-rich plasma.
  
  
  CONCLUSIONS
  Based on the obtained results, the following conclusions were formulated: fluoride may affect NHE activity in non-stimulated human platelets; changes in NHE activity depend on fluoride dose and exposure time; as fluoride concentration and/or exposure time is increased, NHE activity is inhibited; inhibition of NHE activity results in the accumulation of protons inside the platelets and a reduction in intracellular pH (intracellular acidification); the accumulation of protons inside the platelets is accompanied by an increase in intracellular calcium concentration.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Gassowska
authors.forename: Izabela
authors.initials: I
authors.lastname: Gutowska
authors.forename: Irena
authors.initials: I
authors.lastname: Baranowska-Bosiacka
authors.forename: Dariusz
authors.initials: D
authors.lastname: Chlubek
mesh_terms: D000136:Acid-Base Equilibrium / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D005459:Fluorides / Q000494:pharmacology*; D064368:Healthy Volunteers; D006801:Humans; D053657:Platelet-Rich Plasma / Q000187:drug effects / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D017923:Sodium-Hydrogen Exchangers; D005459:Fluorides