title: Deubiquitinating enzymes in oocyte maturation, fertilization and preimplantation embryo development.
abstract: Post-translational modifications of cellular proteins by ubiquitin and ubiquitin-like protein modifiers are important regulatory events involved in diverse aspects of gamete and embryo physiology including oocyte maturation, fertilization and development of embryos to term. Deubiquitinating enzymes (DUBs) regulate proteolysis by reversing ubiquitination, which targets proteins to the 26S proteasome. The ubiquitin C-terminal hydrolases (UCHs) comprise are DUBs that play a role in the removal of multi-ubiquitin chains. We review here the roles of UCHs in oocytes maturation, fertilization and development in mouse, bovine, porcine and rhesus monkeys. Oocyte UCHs contributes to fertilization and embryogenesis by regulating the physiology of the oocyte and blastomere cortex as well as oocyte spindle. Lack of UCHs in embryos reduces fertilization, while mutant embryos fail to undergo compaction and blastocyst formation. In addition to advancing our understanding of reproductive process, research on the role of deubiquitinating enzymes will allow us to better understand and treat human infertility, and to optimize reproductive performance in agriculturally important livestock species.
authors.affiliation: MOFA Global, International Center For Biotechnology (ICB), 2633 State Road 78, Mount Horeb, WI, USA, namdori@gmail.com.
authors.forename: Namdori R
authors.initials: NR
authors.lastname: Mtango
authors.forename: Keith E
authors.initials: KE
authors.lastname: Latham
authors.forename: Peter
authors.initials: P
authors.lastname: Sutovsky
mesh_terms: D000818:Animals; D047108:Embryonic Development / Q000502:physiology*; D005306:Fertilization / Q000502:physiology*; D006801:Humans; D009865:Oocytes / Q000502:physiology*; D046988:Proteasome Endopeptidase Complex / Q000502:physiology; D043222:Ubiquitin Thiolesterase / Q000502:physiology*; D054875:Ubiquitination
chemical_list: D043222:Ubiquitin Thiolesterase; D046988:Proteasome Endopeptidase Complex