title: Immune-mediated experimental arthritis in IL-33 deficient mice.
abstract: Previous work suggested implication of the interleukin (IL)-1 family cytokine IL-33, signaling through its receptor ST2, in the pathogenesis of human and mouse arthritis. In this study, we directly investigated the role of endogenous IL-33 in antigen-induced arthritis (AIA) and collagen-induced arthritis (CIA) using IL-33 KO mice. AIA was induced by injection of methylated bovine serum albumin (mBSA) into knee joints of previously immunized mice. CIA was induced by immunization with bovine type II collagen. Disease severity was evaluated by clinical and histological scoring and cellular immune responses were assessed in cultured draining lymph node cells. Our results indicate that the development of AIA or CIA, as assessed by clinical or histological evaluation, is not impaired in IL-33 deficient mice. We did not observe any consistent modifications in humoral or cellular immune responses in IL-33 KO mice, although IL-33 deficiency enhanced antigen-specific IFN-γ production, proliferation or IgG2a titers in some experiments, suggesting that endogenous IL-33 may contribute to shaping the adaptive immune response. In conclusion, our data suggest that IL-33 plays a modifying rather than a pivotal role in disease development in two models of immune-mediated arthritis.
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, University Hospital, Geneva, Switzerland; Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland.
authors.forename: Dominique
authors.initials: D
authors.lastname: Talabot-Ayer
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, University Hospital, Geneva, Switzerland; Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland.
authors.forename: Praxedis
authors.initials: P
authors.lastname: Martin
authors.affiliation: Novartis Pharma AG, Basel, Switzerland.
authors.forename: Christian Alexander
authors.initials: CA
authors.lastname: Seemayer
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, University Hospital, Geneva, Switzerland; Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland.
authors.forename: Solenne
authors.initials: S
authors.lastname: Vigne
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, University Hospital, Geneva, Switzerland; Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland.
authors.forename: Céline
authors.initials: C
authors.lastname: Lamacchia
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, University Hospital, Geneva, Switzerland.
authors.forename: Axel
authors.initials: A
authors.lastname: Finckh
authors.affiliation: Department of Clinical Pathology, University Hospital, Geneva, Switzerland.
authors.forename: Essia
authors.initials: E
authors.lastname: Saiji
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, University Hospital, Geneva, Switzerland; Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland.
authors.forename: Cem
authors.initials: C
authors.lastname: Gabay
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, University Hospital, Geneva, Switzerland; Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland. Electronic address: Gaby.Palmer@unige.ch.
authors.forename: Gaby
authors.initials: G
authors.lastname: Palmer
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology*; D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D049109:Cell Proliferation; D024043:Collagen Type II; D004195:Disease Models, Animal; D018450:Disease Progression; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D007378:Interleukins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D018123:Receptors, Interleukin / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D024043:Collagen Type II; C486189:Il1rl1 protein, mouse; C506535:Il33 protein, mouse; D007074:Immunoglobulin G; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D007378:Interleukins; D018123:Receptors, Interleukin; D007371:Interferon-gamma
keywords: Arthritis; Cytokine; Immune response; Joint inflammation