title: Bmi-1 regulates epithelial-to-mesenchymal transition to promote migration and invasion of breast cancer cells.
abstract: Breast cancer is a highly invasive and metastatic disease. Recent studies report that breast cancer cells that have undergo epithelial-to-mesenchymal transition (EMT) obtain malignant characteristic, however, the molecular mechanism underlying this transition are poorly understood. Here, we found that over-expression associated with the process of breast cancer and that high B-cell-specific moloney murine leukemia virus insertion site 1 (Bmi-1) levels predict shorter survival of breast cancer patients. We demonstrate that Bmi-1 regulates EMT and the migration of breast cancer cells. RNA interference-mediated knockdown Bmi-1 expression restored E-cadherin expression and cell-cell junction formation in breast cancer cells, suppressing cell migration and invasion. In contrast, the over-expression of Bmi-1 decreased the expression of the epithelial mark (E-cadherin) but increased the mesenchymal makers (N-cadherin and vimentin) in breast cancer cells.
authors.affiliation: Molecular Medicine and Cancer Research Center, Chongqing Medical University Chongqing, 400016, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Chongqing Medical University Chongqing, 400016, China.
authors.forename: Fangzhou
authors.initials: F
authors.lastname: Song
authors.affiliation: Molecular Medicine and Cancer Research Center, Chongqing Medical University Chongqing, 400016, China.
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Molecular Medicine and Cancer Research Center, Chongqing Medical University Chongqing, 400016, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Molecular Medicine and Cancer Research Center, Chongqing Medical University Chongqing, 400016, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Fan
authors.affiliation: Molecular Medicine and Cancer Research Center, Chongqing Medical University Chongqing, 400016, China.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Wu
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D002469:Cell Separation; D018450:Disease Progression; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D006801:Humans; D015151:Immunoblotting; D009361:Neoplasm Invasiveness / Q000473:pathology; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism*; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D046888:Tissue Array Analysis; D014162:Transfection
chemical_list: C069077:BMI1 protein, human; D034741:RNA, Small Interfering; D063150:Polycomb Repressive Complex 1
keywords: Bmi-1; EMT; breast cancer; invasion; migration