title: Potential of extracts from Saponaria officinalis and Calendula officinalis to modulate in vitro rumen fermentation with respect to their content in saponins.
abstract: Saponins have the potential to favorably modulate rumen fermentation, but there is generally a lack of the chemical structures associated with the described effects. The activity of extracts from Calendula officinalis and Saponaria officinalis in the rumen was evaluated in vitro. The S. officinalis root extract, reduced CH₄ production by 8.5% and increased total VFA concentration by 25.2%. C. officinalis and S. officinalis root extracts and the S. officinalis aerial part extract decreased the acetate to propionate ratio from 8.6 to 17.4%, according to the extract. An HPLC-ELSD analysis indicated that the saponin content ranged from 43.6 to 57.6 mg/g of dry matter (DM) in the C. officinalis extracts and from 224.0 to 693.8 mg/g of DM in the S. officinalis extracts, expressed as the hederacoside C equivalent. Identification of the saponin compounds present in the extracts by HPLC-MS(n) suggested that the saponin profile modulated the biological activities, showing the importance of determining the structure of saponins when evaluating extracts.
authors.affiliation: a SONAS Laboratory , Université d'Angers , SFR Quasav, Angers , France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Budan
authors.forename: Denis
authors.initials: D
authors.lastname: Bellenot
authors.forename: Ingrid
authors.initials: I
authors.lastname: Freuze
authors.forename: Louisa
authors.initials: L
authors.lastname: Gillmann
authors.forename: Pierre
authors.initials: P
authors.lastname: Chicoteau
authors.forename: Pascal
authors.initials: P
authors.lastname: Richomme
authors.forename: David
authors.initials: D
authors.lastname: Guilet
mesh_terms: D000818:Animals; D020838:Calendula / Q000737:chemistry*; D005285:Fermentation / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D012417:Rumen / Q000187:drug effects* / Q000378:metabolism*; D029750:Saponaria / Q000737:chemistry*; D012503:Saponins / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D012503:Saponins
keywords: HPLC; methane; rumen; saponin; volatile fatty acid