title: Epigenetics modifications and Subclinical Atherosclerosis in Obstructive Sleep Apnea: The EPIOSA study.
abstract: BACKGROUND
  Obstructive sleep apnea (OSA) is associated with increased risk for cardiovascular morbidity and mortality. Epidemiological and animal models studies generate hypotheses for innovative strategies in OSA management by interfering intermediates mechanisms associated with cardiovascular complications. We have thus initiated the Epigenetics modification in Obstructive Sleep Apnea (EPIOSA) study (ClinicalTrials.gov identifier: NCT02131610).
  
  
  METHODS
  EPIOSA is a prospective cohort study aiming to recruit 350 participants of caucasian ethnicity and free of other chronic or inflammatory diseases: 300 patients with prevalent OSA and 50 non-OSA subjects. All of them will be follow-up for at least 5 years. Recruitment and study visits are performed in single University-based sleep clinic using standard operating procedures. At baseline and at each one year follow-up examination, patients are subjected to a core phenotyping protocol. This includes a standardized questionnaire and physical examination to determine incident comorbidities and health resources utilization, with a primary focus on cardiovascular events. Confirmatory outcomes information is requested from patient records and the regional Department of Health Services. Every year, OSA status will be assessed by full sleep study and blood samples will be obtained for immediate standard biochemistry, hematology, inflammatory cytokines and cytometry analysis. For biobanking, aliquots of serum, plasma, urine, mRNA and DNA are also obtained. Bilateral carotid echography will be performed to assess subclinical atherosclerosis and atherosclerosis progression. OSA patients are treated according with national guidelines.
  
  
  CONCLUSIONS
  EPIOSA will enable the prospective evaluation of inflammatory and epigenetics mechanism involved in cardiovascular complication of treated and non-treated patients with OSA compared with non OSA subjects.
authors.affiliation: Respiratory Service, Hospital Universitario Miguel Servet, I-3 Avda Isabel la Católica, 50006 Zaragoza, Spain. jmmarint@unizar.es.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Marin
authors.forename: Jorge
authors.initials: J
authors.lastname: Artal
authors.forename: Teresa
authors.initials: T
authors.lastname: Martin
authors.forename: Santiago J
authors.initials: SJ
authors.lastname: Carrizo
authors.forename: Marta
authors.initials: M
authors.lastname: Andres
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Martin-Burriel
authors.forename: Rosa
authors.initials: R
authors.lastname: Bolea
authors.forename: Arianne
authors.initials: A
authors.lastname: Sanz
authors.forename: Luis
authors.initials: L
authors.lastname: Varona
authors.forename: Javier
authors.initials: J
authors.lastname: Godino
authors.forename: Begoña
authors.initials: B
authors.lastname: Gallego
authors.forename: Jose A
authors.initials: JA
authors.lastname: Garcia-Erce
authors.forename: Isabel
authors.initials: I
authors.lastname: Villar
authors.forename: Victoria
authors.initials: V
authors.lastname: Gil
authors.forename: Marta
authors.initials: M
authors.lastname: Forner
authors.forename: Jose P
authors.initials: JP
authors.lastname: Cubero
authors.forename: Luis
authors.initials: L
authors.lastname: Ros
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D002340:Carotid Artery Diseases / Q000000981:diagnostic imaging / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D015870:Gene Expression; D006801:Humans; D008137:Longitudinal Studies; D035683:MicroRNAs / Q000032:analysis; D008875:Middle Aged; D017286:Polysomnography; D011446:Prospective Studies; D012333:RNA, Messenger / Q000032:analysis*; D012107:Research Design; D020181:Sleep Apnea, Obstructive / Q000235:genetics* / Q000378:metabolism*; D011795:Surveys and Questionnaires; D014463:Ultrasonography; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D012333:RNA, Messenger; D004247:DNA