title: Rapid determination of vitamin D₃ in milk-based infant formulas by liquid chromatography-tandem mass spectrometry.
abstract: A rapid and simple sample preparation method for vitamin D3 (cholecalciferol) was developed for emulsified dairy products such as milk-based infant formulas. A sample was mixed in a 50 mL centrifuge tube with the same amount of water and isopropyl alcohol to achieve chemical extraction. Ammonium sulfate was used to induce phase separation. No-heating saponification was performed in the sample tube by adding KOH, NaCl, and NH3. Vitamin D3 was then separated and quantified using liquid chromatography-tandem mass spectrometry. The results for added recovery tests were in the range 93.11-110.65%, with relative standard deviations between 2.66% and 2.93%. The results, compared to those obtained using a certified reference material (SRM 1849a), were within the range of the certificated values. This method could be implemented in many laboratories that require time and labour saving.
authors.affiliation: Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd, Sejong 314-914, Republic of Korea.
authors.forename: Byung-Man
authors.initials: BM
authors.lastname: Kwak
authors.affiliation: Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd, Sejong 314-914, Republic of Korea.
authors.forename: In-Seek
authors.initials: IS
authors.lastname: Jeong
authors.affiliation: Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd, Sejong 314-914, Republic of Korea.
authors.forename: Moon-Seok
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd, Sejong 314-914, Republic of Korea. Electronic address: ppori5470@hotmail.com.
authors.forename: Jang-Hyuk
authors.initials: JH
authors.lastname: Ahn
authors.affiliation: Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd, Sejong 314-914, Republic of Korea.
authors.forename: Jong-Su
authors.initials: JS
authors.lastname: Park
mesh_terms: D000818:Animals; D002762:Cholecalciferol / Q000032:analysis*; D002853:Chromatography, Liquid; D041943:Infant Formula / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D012016:Reference Values; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D002762:Cholecalciferol
keywords: Cholecalciferol; LC–MS/MS; Milk-based infant formula; No-heating saponification; Vitamin D(3); dSPE