title: Genetic mutation analysis of human gastric adenocarcinomas using ion torrent sequencing platform.
abstract: Gastric cancer is the one of the major causes of cancer-related death, especially in Asia. Gastric adenocarcinoma, the most common type of gastric cancer, is heterogeneous and its incidence and cause varies widely with geographical regions, gender, ethnicity, and diet. Since unique mutations have been observed in individual human cancer samples, identification and characterization of the molecular alterations underlying individual gastric adenocarcinomas is a critical step for developing more effective, personalized therapies. Until recently, identifying genetic mutations on an individual basis by DNA sequencing remained a daunting task. Recent advances in new next-generation DNA sequencing technologies, such as the semiconductor-based Ion Torrent sequencing platform, makes DNA sequencing cheaper, faster, and more reliable. In this study, we aim to identify genetic mutations in the genes which are targeted by drugs in clinical use or are under development in individual human gastric adenocarcinoma samples using Ion Torrent sequencing. We sequenced 737 loci from 45 cancer-related genes in 238 human gastric adenocarcinoma samples using the Ion Torrent Ampliseq Cancer Panel. The sequencing analysis revealed a high occurrence of mutations along the TP53 locus (9.7%) in our sample set. Thus, this study indicates the utility of a cost and time efficient tool such as Ion Torrent sequencing to screen cancer mutations for the development of personalized cancer therapy.
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Huo
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ye
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Chuanning
authors.initials: C
authors.lastname: Tang
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
authors.forename: Vijayalakshmi
authors.initials: V
authors.lastname: Nandakumar
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lou
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Dong
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Shouwen
authors.initials: S
authors.lastname: Jiang
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Guangchun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Zhishou
authors.initials: Z
authors.lastname: Dong
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Baishuai
authors.initials: B
authors.lastname: Guo
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Chaowei
authors.initials: C
authors.lastname: Yan
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Su
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Dongying
authors.initials: D
authors.lastname: Gu
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Hong
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Yangmei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Cuiju
authors.initials: C
authors.lastname: Tang
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
authors.forename: Lindsey
authors.initials: L
authors.lastname: Jones
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
authors.forename: Xue F
authors.initials: XF
authors.lastname: Huang
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
authors.forename: Si-Yi
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Department of Oncology, The Affiliated Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Jinfei
authors.initials: J
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004252:DNA Mutational Analysis / Q000379:methods; D005091:Exons; D005260:Female; D056426:Genetic Loci; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D059645:Mutation Rate; D020125:Mutation, Missense; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53