title: Molecular diagnostics of Drosophila suzukii (Diptera: Drosophilidae) Using PCR-RFLP.
abstract: The invasive spotted wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), has become a serious pest in the United States. Identification of immature and poorly preserved specimens can be difficult. A molecular diagnostic method for distinguishing D. suzukii from other Drosophila spp. associated with fruit in the United States was developed. A 709-bp region of the mitochondrial DNA cytochrome oxidase I gene was amplified from D. suzukii collections in the United States and compared with sequences of other Drosophila taxa from GenBank. Based on DNA sequence polymorphisms, a polymerase chain reaction-restriction fragment length polymorphism analysis using the restriction enzyme Msp-I was found to differentiate D. suzukii from other Drosophila spp. in the United States. This technique can identify field-collected specimens from various sources and specimens regardless of life stage. This molecular diagnostic method will be useful for monitoring the spread of this economically important invasive insect.
authors.forename: Sh S
authors.initials: ShS
authors.lastname: Kim
authors.forename: A D
authors.initials: AD
authors.lastname: Tripodi
authors.forename: D T
authors.initials: DT
authors.lastname: Johnson
authors.forename: A L
authors.initials: AL
authors.lastname: Szalanski
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011679:Pupa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D019476:Insect Proteins; D003576:Electron Transport Complex IV