title: Mesoscopic reflectance angular domain spectroscopic imaging.
abstract: The advancement of angular domain imaging in mesoscopic reflectance multispectral imaging is reported. The key component is an angular filter array that performs the angular filtration of the back-scattered photons and generates image contrast due to the variances in tissue optical properties. The proposed modality enables multispectral imaging of subsurface features for samples too thick for transillumination angular domain spectroscopic imaging (ADSI) approaches. The validation was carried out with tissue-mimicking phantoms with multiple absorptive features embedded below the surface. Multispectral images in the range of 666 to 888 nm clearly revealed the location of the features with the background scattering levels up to 20 cm⁻¹. The shape of the features was recoverable at depths of up to three to four times the transport mean free path. The spatial resolution was <1 mm and the field-of-view was larger than 2.5 cm x 30. cm. Furthermore, the attenuation spectra of measured absorptive features were successfully extracted. Target detectability and imaging quality with different background scattering levels, target depths, and illumination focal depths were discussed, as well as the capability of ADSI in reflectance optical mesoscopic imaging and its potential applications.
authors.affiliation: Simon Fraser University, School of Engineering Science, Burnaby, British Columbia V5S 1S6, Canada.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Simon Fraser University, School of Engineering Science, Burnaby, British Columbia V5S 1S6, CanadabLawson Health Research Institute, Imaging Program, St. Joseph's Health Care, London, Ontario N6A 4V2, Canada.
authors.forename: Fartash
authors.initials: F
authors.lastname: Vasefi
authors.affiliation: Lawson Health Research Institute, Imaging Program, St. Joseph's Health Care, London, Ontario N6A 4V2, CanadacUniversity of Western Ontario, Schulich School of Medicine and Dentistry, Department of Medical Biophysics, London, Ontario N6A 3K7, Canada.
authors.forename: Eldon
authors.initials: E
authors.lastname: Ng
authors.affiliation: Lawson Health Research Institute, Imaging Program, St. Joseph's Health Care, London, Ontario N6A 4V2, Canada.
authors.forename: Astrid
authors.initials: A
authors.lastname: Chamson-Reig
authors.affiliation: Simon Fraser University, School of Engineering Science, Burnaby, British Columbia V5S 1S6, Canada.
authors.forename: Bozena
authors.initials: B
authors.lastname: Kaminska
authors.affiliation: Lawson Health Research Institute, Imaging Program, St. Joseph's Health Care, London, Ontario N6A 4V2, CanadacUniversity of Western Ontario, Schulich School of Medicine and Dentistry, Department of Medical Biophysics, London, Ontario N6A 3K7, Canada.
authors.forename: Jeffrey J L
authors.initials: JJ
authors.lastname: Carson
mesh_terms: D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D004655:Emulsions / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008954:Models, Biological; D019047:Phantoms, Imaging; D010743:Phospholipids / Q000737:chemistry; D012867:Skin / Q000737:chemistry; D013024:Soybean Oil / Q000737:chemistry; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D004655:Emulsions; D010743:Phospholipids; C545823:soybean oil, phospholipid emulsion; D013024:Soybean Oil