title: In vitro toxicity of grey MTA in comparison to white MTA on human periodontal ligament fibroblasts.
abstract: OBJECTIVE
  This was to define and compare the in vitro toxicity of grey MTA with that of white MTA on cultured human periodontal ligament (PDL) fibroblasts.
  
  
  METHODS
  PDL cells were obtained from sound first permanent molars and cultured in Dulbecco's Modified Eagle's Medium. Cultures were subjected to different concentrations of grey and white MTA (0.5, 5, 50 and 500 µg/ml) for 24 h at 37 °C. Cells that were not exposed to grey or white MTA served as the negative control. In vitro toxicity was assessed using MTT assay.
  
  
  METHODS
  The results were compared using ANOVA and Tukey statistical tests (p < 0.05).
  
  
  RESULTS
  White MTA presented higher in vitro toxicity than grey MTA. However, the differences were almost insignificant (p > 0.05).
  
  
  CONCLUSIONS
  Both colours of MTA are biocompatible since they were both able to preserve PDL fibroblasts for up to 24 h. MTA is as a promising alternative in pulpotomy of primary teeth.
authors.affiliation: Department of Orthodontics and Paediatric Dentistry, Faculty of Dentistry, Qassim University, PO Box 1126, Qassim, 51431, Kingdom of Saudi Arabia, dr.sanaa.alhajali@qudent.org.
authors.forename: S N
authors.initials: SN
authors.lastname: Al-Haj Ali
authors.forename: S H
authors.initials: SH
authors.lastname: Al-Jundi
authors.forename: D J
authors.initials: DJ
authors.lastname: Ditto
mesh_terms: D017607:Aluminum Compounds / Q000008:administration & dosage / Q000633:toxicity*; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000633:toxicity; D001729:Bismuth / Q000008:administration & dosage / Q000633:toxicity*; D017610:Calcium Compounds / Q000008:administration & dosage / Q000633:toxicity*; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D004396:Coloring Agents; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D010087:Oxides / Q000008:administration & dosage / Q000633:toxicity*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000008:administration & dosage / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004396:Coloring Agents; D004338:Drug Combinations; C475480:MTA-Angelus; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; C086631:mineral trioxide aggregate; C022616:thiazolyl blue; D001729:Bismuth