title: Telomere-mediated chromosomal truncation in Aspergillus oryzae.
abstract: We truncated the short arm of chromosome 3 to delete the aflatoxin biosynthesis gene homolog cluster using telomeric repeats in Aspergillus oryzae. The predicted deletion was confirmed by Southern blot analyses. This telomere-mediated chromosomal truncation method enables the development of an artificial chromosome in A. oryzae.
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Sawaki
authors.initials: S
authors.lastname: Tada
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Ohkuchi
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Matsushita-Morita
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Ikuyo
authors.initials: I
authors.lastname: Furukawa
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Hattori
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Fermentation Science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kashiwagi
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. Electronic address: kusumoto@affrc.go.jp.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Kusumoto
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis; D001236:Aspergillus oryzae / Q000235:genetics*; D022201:Chromosomes, Artificial / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000235:genetics*; D017353:Gene Deletion; D005810:Multigene Family / Q000235:genetics; D016615:Telomere / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000348:Aflatoxins
keywords: Aflatoxin; Aspergillus oryzae; Chromosomal truncation; Filamentous fungi; Telomere; Telomere position effect