title: Oil extraction from algae: A comparative approach.
abstract: In this article, various methods including soxhlet, Bligh & Dyer (B&D), and ultrasonic-assisted B&D were investigated for the extraction of lipid from algal species Chlorella vulgaris. Relative polarity/water content and impolar per polar ratios of solvents were considered to optimize the relative proportions of each triplicate agent by applying the response surface method (RSM). It was found that for soxhlet, hexane-methanol (54-46%, respectively) with total lipid extraction of 14.65% and chloroform-methanol (54-46%, respectively) with the extraction of 19.87% lipid were the best set of triplicate where further addition of acetone to the first group and ethanol to the second group did not contributed to further extraction. In B&D, however, chloroform-methanol-water (50%-35%-15%, respectively) reached the all-time maximum of 24%. Osmotic shock as well as ultrasonication contributed to 3.52% of further extraction, which is considered to promote the total yield up to almost 15%. From the growth data and fatty acid analysis, the applied method was assessed to be appropriate for biodiesel production with regard to selectivity and extraction yield.
authors.affiliation: Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
authors.forename: Mehrab
authors.initials: M
authors.lastname: Valizadeh Derakhshan
authors.affiliation: Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
authors.forename: Bahram
authors.initials: B
authors.lastname: Nasernejad
authors.affiliation: Department of Chemical Engineering, Sahand University of Technology, Sahand New-Town, Tabriz, Iran.
authors.forename: Farzin
authors.initials: F
authors.lastname: Abbaspour-Aghdam
authors.affiliation: Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hamidi
mesh_terms: D056804:Biofuels; D005591:Chemical Fractionation / Q000379:methods; D048408:Chlorella vulgaris / Q000737:chemistry*; D010938:Plant Oils / Q000302:isolation & purification*; D013010:Sonication / Q000379:methods*
chemical_list: D056804:Biofuels; D010938:Plant Oils
keywords: Chlorella vulgaris; biodiesel; oil extraction; optimization; sonication