title: Use of the BacT/alert system for rapid detection of microbial contamination in a pilot study using pancreatic islet cell products.
abstract: At the Islet Isolation Laboratory of the Scottish National Blood Transfusion Service, manual sterility testing data show that contamination rates are 57.7% for pancreas transport fluid, 4.3% for postpurification islet samples, and 0% for pretransplant islet samples. This pilot study presents the BacT/Alert System as an alternative to manual testing to provide more rapid and sensitive sterility results for islet cell products.
authors.affiliation: Donation Testing Department, Scottish National Blood Transfusion Service, Glasgow, Scotland.
authors.forename: Laura
authors.initials: L
authors.lastname: Murray
authors.affiliation: SNBTS Cell Therapy Development Centre, Scottish National Blood Transfusion Service, The University of Edinburgh, Edinburgh bioQuarter, Edinburgh, Scotland.
authors.forename: Neil
authors.initials: N
authors.lastname: McGowan
authors.affiliation: Donation Testing Department, Scottish National Blood Transfusion Service, Glasgow, Scotland.
authors.forename: John
authors.initials: J
authors.lastname: Fleming
authors.affiliation: SNBTS Cell Therapy Development Centre, Scottish National Blood Transfusion Service, The University of Edinburgh, Edinburgh bioQuarter, Edinburgh, Scotland Islet Isolation Laboratory, Tissues & Cells Directorate, Scottish National Blood Transfusion Service, Edinburgh, Scotland laurabailey2@nhs.net.
authors.forename: Laura
authors.initials: L
authors.lastname: Bailey
mesh_terms: D058491:Bacterial Load / Q000379:methods*; D001688:Biological Products; D004340:Drug Contamination; D006801:Humans; D007515:Islets of Langerhans; D010865:Pilot Projects; D013997:Time Factors
chemical_list: D001688:Biological Products