title: Mapping of quantitative trait locus (QTLs) that contribute to germination and early seedling drought tolerance in the interspecific cross Setaria italica×Setaria viridis.
abstract: Drought tolerance is an important breeding target for enhancing the yields of grain crop species in arid and semi-arid regions of the world. Two species of Setaria, domesticated foxtail millet (S. italica) and its wild ancestor green foxtail (S. viridis) are becoming widely adopted as models for functional genomics studies in the Panicoid grasses. In this study, the genomic regions controlling germination and early seedling drought tolerance in Setaria were identified using 190 F7 lines derived from a cross between Yugu1, a S. italica cultivar developed in China, and a wild S. viridis genotype collected from Uzbekistan. Quantitative trait loci were identified which contribute to a number of traits including promptness index, radical root length, coleoptile length and lateral root number at germinating stage and seedling survival rate was characterized by the ability of desiccated seedlings to revive after rehydration. A genetic map with 128 SSR markers which spans 1293.9 cM with an average of 14 markers per linkage group of the 9 linkage groups was constructed. A total of eighteen QTLs were detected which included nine that explained over 10% of the phenotypic variance for a given trait. Both the wild green foxtail genotype and the foxtail millet cultivar contributed the favorite alleles for traits detected in this trial, indicating that wild Setaria viridis populations may serve as a reservoir for novel stress tolerance alleles which could be employed in foxtail millet breeding.
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China; College of Life Sciences, Hebei Normal University, Shijiazhuang, China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Qie
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Guanqing
authors.initials: G
authors.lastname: Jia
authors.affiliation: Institute of Dry Land Agriculture, Hebei Academy of Agricultural and Forestry Sciences, Hengshui, China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: James
authors.initials: J
authors.lastname: Schnable
authors.affiliation: College of Life Sciences, Hebei Normal University, Shijiazhuang, China.
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Shang
authors.affiliation: Institute of Millet Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Dry Land Agriculture, Hebei Academy of Agricultural and Forestry Sciences, Hengshui, China.
authors.forename: Binhui
authors.initials: B
authors.lastname: Liu
authors.affiliation: Institute of Dry Land Agriculture, Hebei Academy of Agricultural and Forestry Sciences, Hengshui, China.
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhi
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China; Institute of Millet Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, China; College of Life Sciences, Hebei Normal University, Shijiazhuang, China.
authors.forename: Xianmin
authors.initials: X
authors.lastname: Diao
mesh_terms: D002874:Chromosome Mapping; D055864:Droughts; D002523:Edible Grain / Q000235:genetics / Q000502:physiology; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant; D018525:Germination / Q000235:genetics; D000069600:Plant Breeding; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D040641:Quantitative Trait Loci / Q000235:genetics*; D036226:Seedlings / Q000235:genetics; D032983:Setaria Plant / Q000235:genetics* / Q000502:physiology*; D013312:Stress, Physiological / Q000235:genetics* / Q000502:physiology; D015996:Survival Rate
chemical_list: D005819:Genetic Markers