title: Metabolic engineering of Escherichia coli for poly(3-hydroxypropionate) production from glycerol and glucose.
abstract: A new poly(3-hydroxypropionate) (P3HP) biosynthetic pathway employing β-alanine as an intermediate from an inexpensive carbon source was developed in recombinant Escherichia coli. After a series of systematic optimization, the genes for L-aspartate decarboxylase and its maturation factor (panD and panM, from E. coli), β-alanine-pyruvate transaminase (pp0596, from Pseudomonas putida), 3-hydroxy acid dehydrogenase and 3-hydroxypropionyl-CoA synthase (ydfG and prpE respectively, from E. coli), and polyhydroxyalkanoate synthase (phaC1, from Cupriavidus necator) were cloned and expressed in E. coli. Under shake-flask conditions, the recombinant strain produced 0.5 g P3HP l(-1) from glycerol and glucose, up to 10.2 % of CDW. Though the content of P3HP was low, this pathway has some advantages over other reported pathways, such as being redox neutral, does not require any coenzyme, and can use a wide range of carbon sources.
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.forename: Lu
authors.initials: L
authors.lastname: Feng
authors.forename: Jiming
authors.initials: J
authors.lastname: Wang
authors.forename: Guang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D001709:Biotechnology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D054813:Polyhydroxyalkanoates / Q000032:analysis / Q000378:metabolism*
chemical_list: D054813:Polyhydroxyalkanoates; D005947:Glucose; D005990:Glycerol