title: Changes in the expression of epidermal differentiation markers at sites where cultured epithelial autografts were transplanted onto wounds from burn scar excision.
abstract: This study investigated the recovery process during which grafted cultured epithelium formed normal epidermis. The subjects were 18 patients whose burn scars were excised at a depth not exposing the fat layer and who subsequently received cultured epithelial autografts. A total of 24 samples were obtained from the grafted sites: 6 samples within 6 weeks (stage 1), 5 samples after 6 weeks and within 6 months (stage 2), 6 samples after 6 months and within 18 months (stage 3) and 7 samples beyond 18 months (stage 4) after transplantation. These samples were stained for monoclonal antibodies against filaggrin, transglutaminase (TG), cytokeratin 6 and involucrin. Their expressions were examined in the epidermis. The expression patterns were classified using a six-grade scale. The grades of filaggrin and TG were significantly higher at stage 3 and 4 compared with stage 1. There was a marginally significant increase in the grade of cytokeratin 6 at stage 3 and it was significantly higher at stage 4 compared with stage 1. These results showed that wound healing continued at a molecular level until the end of stage 3. The recovery of involucrin was delayed compared with that of other markers. TG and involucrin are thought to be regulated independently at the grafted sites.
authors.affiliation: Shiseido Research Center, Yokohama, Japan.
authors.forename: Kuniko
authors.initials: K
authors.lastname: Kadoya
authors.affiliation: Shiseido Research Center, Yokohama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Amano
authors.affiliation: Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Nishiyama
authors.affiliation: Shiseido Research Center, Yokohama, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Inomata
authors.affiliation: Shiseido Research Center, Yokohama, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsunenaga
authors.affiliation: Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Kumagai
authors.affiliation: Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Kawasaki, Japan.|Department of Plastic and Reconstructive Surgery, Kawasaki Municipal Tama Hospital, Kawasaki, Japan.
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Matsuzaki
mesh_terms: D000943:Antigens, Differentiation; D064592:Autografts; D002056:Burns; D002454:Cell Differentiation; D002478:Cells, Cultured; D002921:Cicatrix; D004817:Epidermis; D000091344:Filaggrin Proteins; D006801:Humans; D016038:Skin Transplantation
chemical_list: D000943:Antigens, Differentiation; C000717249:FLG protein, human; D000091344:Filaggrin Proteins
keywords: Burn scar; Cultured epithelium; Epidermal differentiation marker; Wound