title: Trichoderma reesei XYN VI--a novel appendage-dependent eukaryotic glucuronoxylan hydrolase.
abstract: Expression of a Trichoderma reesei gene coding for a putative GH30 xylanase in Aspergillus oryzae led to isolation and purification of a novel xylanase exhibiting catalytic properties different from those of the previously characterized GH30 xylanase XYN IV of T. reesei. The novel enzyme, named XYN VI, exhibited catalytic properties similar to appendage-dependent GH30 glucuronoxylanases previously recognized only in bacteria. XYN VI showed high specific activity only on xylans or xylooligosaccharides containing 4-O-methyl-D-glucuronic acid or D-glucuronic acid side substituents. The cleavage of the main chain takes place primarily at the second glycosidic linkage from the branch towards the reducing end of the polysaccharides or aldouronic acids. These catalytic properties resemble bacterial GH30 glucuronoxylanases, although the recognition of the uronic acid side chains by XYN VI is apparently based on interaction of the substrate with other amino acids. Moreover, in contrast to bacterial enzymes, XYN VI is also capable of slower but significant cleavage of unsubstituted parts of xylan and acidic xylooligosaccharides. The data point to a great catalytic diversity of xylanases produced by the most extensively studied cellulolytic fungus.
authors.affiliation: Institute of Chemistry, Center of Glycomics, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Biely
authors.forename: Vladimír
authors.initials: V
authors.lastname: Puchart
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Stringer
authors.forename: Kristian B R
authors.initials: KB
authors.lastname: Mørkeberg Krogh
mesh_terms: D000595:Amino Acid Sequence; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D016415:Sequence Alignment; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology; D014990:Xylans / Q000378:metabolism*; D014995:Xylosidases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014990:Xylans; C038910:glucuronoxylan; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases
keywords: Trichoderma reesei; XYN VI; aldouronic acids; glucuronoxylan; novel GH30 xylanase; plant cell walls; xylooligosaccharides