title: (1)H, (15)N and (13)C resonance assignments and secondary structure prediction of Q4D059, a conserved and kinetoplastid-specific hypothetical protein from Trypanosoma cruzi.
abstract: Trypanosoma cruzi is a human parasite that causes Chagas disease, an illness affecting millions of people and without an efficient treatment available. Sequencing the pathogen genome has revealed that near half of protein-coding genes correspond to hypothetical proteins of unknown function, increasing the possibilities for novel target discovery. Q4D059 is a putative essential hypothetical protein from T. cruzi and it is specific and conserved among the trypanosomatid genomes. Here, we report the sequential backbone and side chain resonance assignments and secondary structure analysis of Q4D059, as first step for protein structure determination, function elucidation and drug screening.
authors.affiliation: Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Brigadeiro Trompowiski s/n CCS, Bloco E, sala 10, Rio de Janeiro, RJ, 21941-590, Brazil.
authors.forename: Aracelys
authors.initials: A
authors.lastname: López-Castilla
authors.forename: Rachel S
authors.initials: RS
authors.lastname: de Menezes
authors.forename: Éverton D
authors.initials: ÉD
authors.lastname: D' Andrea
authors.forename: Thamires R
authors.initials: TR
authors.lastname: dos Santos
authors.forename: José R
authors.initials: JR
authors.lastname: Pires
mesh_terms: D017124:Conserved Sequence; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D015800:Protozoan Proteins / Q000737:chemistry*; D014349:Trypanosoma cruzi / Q000166:cytology*
chemical_list: D015800:Protozoan Proteins