title: Quantitative fluorometric assay for the measurement of endo-1,4-β-glucanase.
abstract: There is a growing demand for research tools to aid the scientific community in the search for improved cellulase enzymes for the biofuel industry. In this work, we describe a novel fluorometric assay for cellulase (endo-1,4-β-glucanase) which is based on the use of 4,6-O-benzylidene-4-methylumbelliferyl-β-cellotrioside (BzMUG3) in the presence of an ancillary β-glucosidase. This assay can be used quantitatively over a reasonable linear range, or qualitatively as a solution screening tool which may find extensive use in the area of metagenomics.
authors.affiliation: Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, Ireland. Electronic address: david@megazyme.com.
authors.forename: D
authors.initials: D
authors.lastname: Mangan
authors.affiliation: Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, Ireland.
authors.forename: B V
authors.initials: BV
authors.lastname: McCleary
authors.affiliation: Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, Ireland.
authors.forename: A
authors.initials: A
authors.lastname: Liadova
authors.affiliation: Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, Ireland.
authors.forename: R
authors.initials: R
authors.lastname: Ivory
authors.affiliation: Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, Ireland.
authors.forename: N
authors.initials: N
authors.lastname: McCormack
mesh_terms: D001597:Benzylidene Compounds / Q000737:chemistry; D001681:Biological Assay; D002480:Cellulase / Q000032:analysis* / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D057075:Enzyme Assays; D005456:Fluorescent Dyes / Q000737:chemistry; D005470:Fluorometry / Q000379:methods*; D005656:Fungal Proteins / Q000032:analysis* / Q000737:chemistry; D006923:Hymecromone / Q000737:chemistry*; D007700:Kinetics; D014242:Trichoderma / Q000737:chemistry / Q000201:enzymology; D001617:beta-Glucosidase / Q000737:chemistry
chemical_list: D001597:Benzylidene Compounds; D005456:Fluorescent Dyes; D005656:Fungal Proteins; D006923:Hymecromone; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase
keywords: 4-Methylumbelliferone; Assay procedure; Cellulase; Fluorometric; Metagenomics; endo-1,4-β-Glucanase