title: A piezoelectric-based immunosensor for high density lipoprotein particle measurement.
abstract: A piezoelectric-based immunosensor was developed for high density lipoprotein particle (HDL-P) measurement. Monoclonal anti-human apolipoprotein A1 antibody was used as a specific binding molecule for the major apolipoprotein of HDL-P. This sensing element was fabricated by immobilizing the anti-human apolipoprotein A1 on a 12 MHz AT-cut quartz crystal via a 3-mercaptopropionic acid (MPA) self-assembled monolayer. The frequency shift from the mass change of the antigen-antibody binding refers to the amount of HDL-P. The optimal antibody immobilization was performed to achieve the maximum potential of the antibody. The appropriate quantity and immobilization time of the antibody were 0.1 mg ml(-1) and 90 minutes, respectively. The immobilized antibody in the HDL-P immunosensor accomplished perfect binding with HDL-P within 60 minutes. The dose-response curve for HDL-P showed a linear response from 0.21 to 2.50 mg protein per ml equivalent to 0.40 × 10(10) to 3.65 × 10(10) particles per μl without significant interference from other lipoproteins. The intra- and inter-assay imprecision (CV) were 7.8 and 18.5%, respectively. The analytical accuracy of this measurement was 96.29-96.31%. The HDL-P concentration obtained from the sensor revealed a 2.05 mg protein per ml with 0.26 mg protein per ml of expanded uncertainty at the 95% confidence level. This immunosensor gave an assay result which correlated with the homogeneous enzymatic colorimetric assay (R(2) = 0.902).
authors.affiliation: Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, 999 Phuttamonthon 4 Road, Salaya, Phuttamonthon, Nakhon Pathom 73170, Thailand. chamras.pro@mahidol.ac.th.
authors.forename: Suticha
authors.initials: S
authors.lastname: Chunta
authors.forename: Jamikorn
authors.initials: J
authors.lastname: Suk-Anake
authors.forename: Kosum
authors.initials: K
authors.lastname: Chansiri
authors.forename: Chamras
authors.initials: C
authors.lastname: Promptmas
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D055910:Antibodies, Immobilized / Q000737:chemistry*; D016632:Apolipoprotein A-I / Q000032:analysis / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation; D003460:Crystallization; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D008075:Lipoproteins, HDL / Q000032:analysis / Q000097:blood*; D011791:Quartz / Q000737:chemistry
chemical_list: C505693:APOA1 protein, human; D055910:Antibodies, Immobilized; D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL; D011791:Quartz; D015097:3-Mercaptopropionic Acid