title: Preparation and characterization of bone marrow mesenchymal stem cell-derived extracellular matrix scaffolds.
abstract: The aim of this study was to assess the feasibility of creating extracellular matrix (ECM) scaffolds from mesenchymal stem cells. Bone marrow mesenchymal stem cell (BMSC)-derived ECM (BMSC-dECM) scaffolds were fabricated by lyophilization after crosslinking, without using a decellularization process. Acellular porcine chondrocyte-derived ECM (AC-dECM) scaffolds were used as a control. The surface morphology, internal structure, water uptake ratio, mechanical properties, and biocompatibility of the scaffolds, as well as the in vitro behavior of cells grown on the scaffolds were examined and compared between the two scaffold types. For the BMSC-dECM scaffolds, the average pore size was 304.4 ± 108.2 μm, average porosity was 93.3% ± 4.5%, average compressive modulus was 6.8 ± 1.5 kPa, and average water uptake ratio exceeded 20. The BMSC-dECM scaffolds supported the in vitro attachment and proliferation of cells, with these aspects likely being comparable to those of the AC-dECM scaffolds. The findings of this preliminary study highlight the potential utility of BMSC-derived ECM scaffolds for future cartilage tissue-engineering applications.
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nanjing Medical University (Nanjing First Hospital), Nanjing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Guang-yue
authors.initials: GY
authors.lastname: Xu
authors.forename: Cheng
authors.initials: C
authors.lastname: Tang
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.forename: Xuan
authors.initials: X
authors.lastname: Pei
authors.forename: Jian-chao
authors.initials: JC
authors.lastname: Gui
authors.forename: Byoung-hyun
authors.initials: BH
authors.lastname: Min
authors.forename: Cheng-zhe
authors.initials: CZ
authors.lastname: Jin
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Wang
mesh_terms: D050156:Adipogenesis; D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology / Q000648:ultrastructure*; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis; D019245:Compressive Strength; D003432:Cross-Linking Reagents; D005109:Extracellular Matrix / Q000648:ultrastructure*; D005240:Feasibility Studies; D005612:Freeze Drying; D057927:Hydrophobic and Hydrophilic Interactions; D059630:Mesenchymal Stem Cells / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D016062:Porosity; D011817:Rabbits; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents
keywords: autologous mesenchymal stem cells; extracellular matrix; porous scaffold; tissue engineering