title: LncRNA TSLC1-AS1 is a novel tumor suppressor in glioma.
abstract: Growing evidence demonstrates that long non coding RNAs (lncRNAs) play an important role in cancer origination and progression. A novel lncRNA, TSLC1-AS1, is the antisense transcript of tumor suppressor TSLC1. The expression profile and function of TSLC1-AS1 in glioma were investigated using Real-Time Quantitative PCR and siRNA knockdown. The data showed that TSLC1-AS1 expression was down-regulated in tumor tissues compared with that in adjacent normal tissues, and negatively associated with the WHO criteria of the tumors. Overexpression of TSLC1-AS1 resulted in up-regulation of TSLC1 and significant inhibition of cell proliferation, migration and invasion in U87 cells, while knockdown of TSLC1-AS1 in SNB-19 cells showed the opposite effect. The expression of TSLC1-AS1 was also positively correlated with other tumor suppressors NF1, VHL, PIK3R1 and negatively correlated with the oncogene BRAF. The results suggested that TSLC1-AS1 was a tumor suppressor of glioma and a mediator of TSLC1 expression. LncRNA TSLC1-AS1 may serve as a potential biomarker and therapeutic target for glioma.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Chinese PLA General Hospital Medical School Beijing 100048, China ; Department of Neurosurgery, 253 Hospital of PLA Huhhot 010051, China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Qin
authors.affiliation: Department of Oncology, 161 Hospital of PLA Jiangan District, Wuhan 430010, Hubei Province, China ; Cancer Center, Chinese PLA General Hospital Medical School Beijing 100853, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yao
authors.affiliation: Cancer Center, Chinese PLA General Hospital Medical School Beijing 100853, China.
authors.forename: Peiliang
authors.initials: P
authors.lastname: Geng
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Chinese PLA General Hospital Medical School Beijing 100048, China.
authors.forename: Xiangping
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Chinese PLA General Hospital Medical School Beijing 100048, China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Xue
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Chinese PLA General Hospital Medical School Beijing 100048, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D005910:Glioma / Q000235:genetics*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome; D014162:Transfection
chemical_list: C403514:CADM1 protein, human; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering
keywords: Long non coding RNAs; glioma; tumor suppressor