title: Down-regulation of miRNA-452 is associated with adriamycin-resistance in breast cancer cells.
abstract: Adriamycin (ADR) is an important chemotherapeutic agent frequently used in treatment of breast cancer. However, resistance to ADR results in treatment failure in many patients. Recent studies have indicated that microRNAs (miRNAs) may play an important role in such drug-resistance. In the present study, microRNA-452 (miR-452) was found to be significantly down-regulated in adriamycin-resistant MCF-7 cells (MCF-7/ADR) compared with the parental MCF-7 cells by miRNA microarray and real-time quantitative PCR (RT-qPCR). MiR-452 mimics and inhibitors partially changed the adriamycin-resistance of breast cancer cells, as also confirmed by apoptosis assay. In exploring the potential mechanisms of miR-452 in the adriamycin-resistance of breast cancer cells, bioinformatics analysis, RT-qPCR and Western blotting showed that dysregulation of miR-452 played an important role in the acquired adriamycin-resistance of breast cancer, maybe at least in part via targeting insulin-like growth factor-1 receptor (IGF-1R).
authors.affiliation: Department of General Surgery, Nanjing Medical University Affiliated Jiangsu Cancer Hospital, Nanjing, China E-mail : doczhaojianhua@163.com, doctangjinhai@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Hu
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Gong
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Shan-Liang
authors.initials: SL
authors.lastname: Zhong
authors.forename: Wei-Xian
authors.initials: WX
authors.lastname: Chen
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Zhang
authors.forename: Teng-Fei
authors.initials: TF
authors.lastname: Ma
authors.forename: Hao
authors.initials: H
authors.lastname: Ji
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Lv
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhao
authors.forename: Jin-Hai
authors.initials: JH
authors.lastname: Tang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics*; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics*; D017526:Receptor, IGF Type 1 / Q000235:genetics
chemical_list: C574051:MIRN452 microRNA, human; D035683:MicroRNAs; D004317:Doxorubicin; D017526:Receptor, IGF Type 1