title: Rat strain differences in levels and effects of chronic inflammation due to intratracheal instillation of quartz on lung tumorigenesis induced by DHPN.
abstract: Chronic inflammatory effects of single intratracheal instillation (i.t.) of quartz on rat lung tumorigenesis were examined using 4 different animal models. At first, in order to determine an appropriate dose of quartz i.t. to promote lung tumorigenesis, F344 male rats were administrated single 0, 0.5, 1, 2 or 4 mg quartz/rat after initiation by N-bis(2-hydroxypropyl) nitrosamine (DHPN). Further studies were performed to examine strain differences of the effects of chronic inflammation caused by quartz i.t. in 3 strains of rat, i.e. F344, Wistar-Hannover and SD. Each was instilled with 2mg quartz/rat after DHPN administration and sacrificed in week 24. In addition, strain differences in generation of inflammation were determined at days 1 and 28. Finally, for determination of long-term effects period, F344 and Wistar-Hannover rats were similarly treated, but the experiment was terminated at week 52. In F344 rats, the tumor areas in DHPN treated groups showed a tendency to increase along with the dose of quartz. F344 rats demonstrated the highest and Wistar-Hannover rats the lowest sensitivity to quartz in acute and chronic phases in the 3 strains. In 52 week, in F344 rats, the multiplicity of tumors and the serum concentration of IL-6 in the group treated with DHPN and quartz were significantly increased. The present experiments indicated that chronic inflammation due to quartz instillation exerted promoting effects on lung carcinogenesis in F344, SD and Wistar-Hannover rats. The strain differences in tumor promotion appeared to correlate with inflammatory reactions to quartz and increase of IL-6.
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nakano
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Masanao
authors.initials: M
authors.lastname: Yokohira
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Hashimoto
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Yamakawa
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Sosuke
authors.initials: S
authors.lastname: Kishi
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Ninomiya
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Kanie
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan; Department of Diagnostic Pathology, Tomakomai City Hospital, Hokkaido 053-8567, Japan.
authors.forename: Kousuke
authors.initials: K
authors.lastname: Saoo
authors.affiliation: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan. Electronic address: imaida@med.kagawa-u.ac.jp.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Imaida
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D002273:Carcinogens / Q000633:toxicity; D007249:Inflammation / Q000139:chemically induced* / Q000150:complications*; D008168:Lung / Q000187:drug effects; D008175:Lung Neoplasms / Q000209:etiology* / Q000235:genetics; D008297:Male; D009602:Nitrosamines / Q000633:toxicity; D011791:Quartz / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
chemical_list: D002273:Carcinogens; D009602:Nitrosamines; D011791:Quartz; C012457:diisopropanolnitrosamine
keywords: Chronic inflammation; DHPN; Lung rat tumorigenesis; Quartz; Strain difference