title: Phylogenetic relationship of Paenibacillus species based on putative replication origin regions and analysis of an yheCD-like sequence found in this region.
abstract: To determine the phylogenetic relationship among Paenibacillus species, putative replication origin regions were compared. In the rsmG-gyrA region, gene arrangements in Paenibacillus species were identical to those of Bacillus species, with the exception of an open reading frame (orf14) positioned between gyrB and gyrA, which was observed only in Paenibacillus species. The orf14 product was homologous to the endospore-associated proteins YheC and YheD of Bacillus subtilis. Phylogenetic analysis based on the YheCD proteins suggested that Orf14 could be categorized into the YheC group. In the Paenibacillus genome, DnaA box clusters were found in rpmH-dnaA and dnaA-dnaN intergenic regions, known as box regions C and R, respectively; this localization was similar to that observed in B. halodurans. A phylogenetic tree based on the nucleotide sequences of the whole replication origin regions suggested that P. popilliae, P. thiaminolyticus, and P. dendritiformis are closely related species.
authors.affiliation: a Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University , Fukuoka , Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Iiyama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Otao
authors.forename: Kazuki
authors.initials: K
authors.lastname: Mori
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Mon
authors.forename: Jae Man
authors.initials: JM
authors.lastname: Lee
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
authors.forename: Kousuke
authors.initials: K
authors.lastname: Tashiro
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Asano
authors.forename: Chisa
authors.initials: C
authors.lastname: Yasunaga-Aoki
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D004261:DNA Replication; D023061:Gene Order; D023281:Genomics; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017421:Sequence Analysis
chemical_list: D001426:Bacterial Proteins
keywords: ATP-grasp superfamily; Paenibacillus species; YheC/D family proteins; phylogenetic relationship; putative replication origin region