title: Gene expression profiling of candidate virulence factors in the laminated root rot pathogen Phellinus sulphurascens.
abstract: BACKGROUND
  Phellinus sulphurascens is a fungal pathogen that causes laminar root rot in conifers, one of the most damaging root diseases in western North America. Despite its importance as a forest pathogen, this fungus is still poorly studied at the genomic level. An understanding of the molecular events involved in establishment of the disease should help to develop new methods for control of this disease.
  
  
  RESULTS
  We generated over 4600 expressed sequence tags from two cDNA libraries constructed using either mycelia grown on cellophane sheets and exposed to Douglas-fir roots or tissues from P. sulphurascens-infected Douglas-fir roots. A total of 890 unique genes were identified from the two libraries, and functional classification of 636 of these genes was possible using the Functional Catalogue (FunCat) annotation scheme. cDNAs were identified that encoded 79 potential virulence factors, including numerous genes implicated in virulence in a variety of phytopathogenic fungi. Many of these putative virulence factors were also among 82 genes identified as encoding putatively secreted proteins. The expression patterns of 86 selected fungal genes over 7 days of infection of Douglas-fir were examined using real-time PCR, and those significantly up-regulated included rhamnogalacturonan acetylesterase, 1,4-benzoquinone reductase, a cyclophilin, a glucoamylase, 3 hydrophobins, a lipase, a serine carboxypeptidase, a putative Ran-binding protein, and two unknown putatively secreted proteins called 1 J04 and 2 J12. Significantly down-regulated genes included a manganese-superoxide dismutase, two metalloproteases, and an unknown putatively secreted protein called Ps0058.
  
  
  CONCLUSIONS
  This first collection of Phellinus sulphurascens EST sequences and its annotation provide an important resource for future research aimed at understanding key virulence factors of this forest pathogen. We examined the expression patterns of numerous fungal genes with potential roles in virulence, and found a collection of functionally diverse genes that are significantly up- or down-regulated during infection of Douglas-fir seedling roots by P. sulphurascens.
authors.affiliation: Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, Victoria V8Z 1M5, BC, Canada. Holly.Williams@NRCan-RNCan.gc.ca.
authors.forename: Holly L
authors.initials: HL
authors.lastname: Williams
authors.forename: Rona N
authors.initials: RN
authors.lastname: Sturrock
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Islam
authors.forename: Craig
authors.initials: C
authors.lastname: Hammett
authors.forename: Abul K M
authors.initials: AK
authors.lastname: Ekramoddoullah
authors.forename: Isabel
authors.initials: I
authors.lastname: Leal
mesh_terms: D001487:Basidiomycota / Q000235:genetics* / Q000378:metabolism; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005787:Gene Frequency; D005800:Genes, Fungal; D058977:Molecular Sequence Annotation; D025282:Mycelium / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D028224:Pseudotsuga / Q000382:microbiology; D059467:Transcriptome; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D037521:Virulence Factors