title: Further validation of unsaturated long-chain fatty acids as inhibitors for oxidized low-density lipoprotein binding to CD36 via assays with synthetic CD36 peptide-cross-linked plates.
abstract: We recently obtained evidence that unsaturated long-chain fatty acids (LCFAs) (e.g. oleic acid) inhibit binding of oxidized low-density lipoproteins (oxLDLs) to CD36. In the present study, we validated this prediction by examining inhibition by unsaturated LCFAs of Alexa-fluor-labeled oxLDL binding to multiwell plates onto which a synthetic CD36 peptide is covalently immobilized via thiol-maleimide coupling.
authors.affiliation: a Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kozai
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsuzuki
authors.forename: Marie
authors.initials: M
authors.lastname: Takai
authors.forename: Ai
authors.initials: A
authors.lastname: Eguchi
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Matsumura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Inoue
authors.forename: Tohru
authors.initials: T
authors.lastname: Fushiki
mesh_terms: D000595:Amino Acid Sequence; D018955:CD36 Antigens / Q000737:chemistry* / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry* / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects
chemical_list: D018955:CD36 Antigens; D005227:Fatty Acids; D008077:Lipoproteins, LDL; D010446:Peptide Fragments; C099252:oxidized low density lipoprotein
keywords: CD36; binding inhibition; oxidized low-density lipoproteins; peptide cross linking; unsaturated long-chain fatty acids