title: Mechanisms of induction of the stress-responsive transcription factors HsfA2 and DREB2A by 12-oxo-phytodienoic acid in Arabidopsis thaliana.
abstract: OPDA (12-oxo-phytodienoic acid) not only is an intermediate in jasmonic acid biosynthesis but also regulates gene expression, although mechanisms of OPDA-induced signaling are largely unknown. Here, we measured transcriptional responses of the OPDA-responsive genes HsfA2 and DREB2A to the protein synthesis inhibitor cycloheximide and to the HSP90 inhibitor geldanamycin. The results suggest that HSP90 and other proteins suppress the expression.
authors.affiliation: a Center for Biological Resources and Informatics, Tokyo Institute of Technology , Yokohama , Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Masuda
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Tokaji
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohta
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics*; D016227:Benzoquinones / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics*; D047029:Lactams, Macrocyclic / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects; D013312:Stress, Physiological / Q000187:drug effects*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation / Q000187:drug effects*
chemical_list: D029681:Arabidopsis Proteins; D016227:Benzoquinones; D004268:DNA-Binding Proteins; C114579:DREB2A protein, Arabidopsis; D005231:Fatty Acids, Unsaturated; C000620134:HSFA2 protein, Arabidopsis; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D010940:Plant Proteins; D014157:Transcription Factors; C025999:12-oxophytodienoic acid; D003513:Cycloheximide; C001277:geldanamycin
keywords: 12-oxo-phytodienoic acid (OPDA); Arabidopsis; HsfA2; Oxylipin; dehydration-responsive element-binding protein (DREB) 2A