title: Complete sequence of pABTJ2, a plasmid from Acinetobacter baumannii MDR-TJ, carrying many phage-like elements.
abstract: Acinetobacter baumannii is an important opportunistic pathogen in hospital, and the multidrug-resistant isolates of A. baumannii have been increasingly reported in recent years. A number of different mechanisms of resistance have been reported, some of which are associated with plasmid-mediated acquisition of genes. Therefore, studies on plasmids in A. baumannii have been a hot issue lately. We have performed complete genome sequencing of A. baumannii MDR-TJ, which is a multidrug-resistant isolate. Finalizing the remaining large scaffold of the previous assembly, we found a new plasmid pABTJ2, which carries many phage-like elements. The plasmid pABTJ2 is a circular double-stranded DNA molecule, which is 110,967bp in length. We annotated 125 CDSs from pABTJ2 using IMG ER and ZCURVE_V, accounting for 88.28% of the whole plasmid sequence. Many phage-like elements and a tRNA-coding gene were detected in pABTJ2, which is rarely reported among A. baumannii. The tRNA gene is specific for asparagine codon GTT, which may be a small chromosomal sequence picked up through incorrect excision during plasmid formation. The phage-like elements may have been acquired during the integration process, as the GC content of the region carrying phage-like elements was higher than that of the adjacent regions. The finding of phage-like elements and tRNA-coding gene in pABTJ2 may provide a novel insight into the study of A. baumannii pan-plasmidome.
authors.affiliation: MOE Key Laboratory of Systems Bioengineering, Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: MOE Key Laboratory of Systems Bioengineering, Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.affiliation: MOE Key Laboratory of Systems Bioengineering, Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Yang
authors.affiliation: Department of Physics, School of Science, Tianjin University, Tianjin 300072, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Physics, School of Science, Tianjin University, Tianjin 300072, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Physics, School of Science, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China. Electronic address: fgao@tju.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001435:Bacteriophages / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012343:RNA, Transfer
keywords: A. baumannii; Phage-like elements; Plasmid; tRNA gene