title: [Detection of HTLV-1 DNA in biopsies of Chilean patients with cutaneous T-cell lymphoma].
abstract: BACKGROUND
  Human T-lymphotropic virus-1 (HTLV-1) infection has been associated with the pathogenesis of cutaneous T cell lymphomas (CTCL).
  
  
  OBJECTIVE
  To search for HTLV-1 DNA in skin biopsies of patients with CTCL.
  
  
  METHODS
  A retrospective study was conducted using 25 biopsies of patients with CTCL. DNA was extracted from lymphoid tissue by microdissection. A nested PCR was conducted to detect HTLV-1 genome using primers for the tax region. As negative controls, four cases of superficial perivascular dermatitis were chosen. As positive controls, five cases of T-cell leukemia/lymphoma (ATCL) were studied.
  
  
  RESULTS
  A positive reaction was found in 3 of 25 cases. These biopsies corresponded to a case of Mycosis Fungoides, a case of CD30 (-) T-cell lymphoma and a case of lymphomatoid papulosis. Search was negative in the four cases of superficial perivascular dermatitis and positive in four cases of adult T-cell leukemia/lymphoma (ATCL).
  
  
  CONCLUSIONS
  HTLV-1 DNA search in tissues is a useful tool recommended to study T-cell lymphomas. HTLV-1 infection only occurs in sporadic cases but may contribute to tumor aggressiveness and prognosis.
authors: Juana
authors: J
authors: Benedetto E
authors: Montserrat
authors: M
authors: Molgó N
authors: Sergio
authors: S
authors: González B
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D016022:Case-Control Studies; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015490:HTLV-I Infections / Q000473:pathology / Q000821:virology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D016410:Lymphoma, T-Cell, Cutaneous / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009182:Mycosis Fungoides / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000473:pathology / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral