title: Purification and characterization of five snake venom metalloproteinases from Egyptian Echis pyramidum pyramidum venom.
abstract: New five P-III snake venom metalloproteinases (SVMPs): EpyB2 (62 kDa), EpyB3 (62+23 kDa), EpyB4 (60 kDa), EpyB5 (67 kDa) and EpyB6 (66 kDa) of the most dangerous viper, Echis pyramidum pyramidum (Epy), were purified and characterized in a set of biochemical assays. The SVMPs were purified by applying a protocol of two successive chromatographic steps. Three purified SVMPs "EpyB2, EpyB4, and EpyB5" have hemorrhagic activity with MHDs, 7 μg, 7.6 μg and 15 μg, respectively; furthermore, they have high preference towards fibronectin, collagen, gelatin, fibrin and hemoglobin substrates compared with non-hemorrhagic SVMPs (EpyB3 and EpyB6). All the purified SVMPs showed remarkable thermal and pH stability, inhibited by metalloproteinase inhibitors and Zn(2+), Mn(2+), Ni(2+), Co(2+), Cu(2+), and Hg(2+). The purified SVMPs act as α-fibrinogenases, prothrombin activators and procoagulants. In conclusion, Epy venom has multiple SVMPs that are responsible for hemorrhagic events and thus represent a significant health hazard for victims of envenomation, however, they may be useful for treating diseases involving abnormal blood clot formation.
authors: Department of Molecular Biology, National Research Center.
authors: Azza M
authors: AM
authors: Abdel-Aty
authors: Ahmed F
authors: AF
authors: Wahby
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002364:Caseins / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D006470:Hemorrhage / Q000139:chemically induced; D006487:Hemostasis / Q000187:drug effects; D008297:Male; D045726:Metalloproteases / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D051379:Mice; D059748:Proteolysis / Q000187:drug effects; D013379:Substrate Specificity; D013696:Temperature; D014757:Viper Venoms / Q000201:enzymology*; D017819:Viperidae
chemical_list: D002364:Caseins; D014757:Viper Venoms; D005780:Gelatin; D005340:Fibrinogen; D045726:Metalloproteases