title: Nano-scale liquid chromatography coupled to tandem mass spectrometry using the multiple reaction monitoring mode based quantitative platform for analyzing multiple enzymes associated with central metabolic pathways of Saccharomyces cerevisiae using ultra fast mass spectrometry.
abstract: A widely targeted quantitative proteome analysis of Saccharomyces cerevisiae enzymes was performed employing an ultra fast mass spectrometry (UFMS). Nano-liquid chromatography-UFMS analysis of trypsin digested peptide samples derived from yeast strains successfully determined relative abundances of 303 peptides of 137 proteins.
authors: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, Japan; RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. Electronic address: fmatsuda@ist.osaka-u.ac.jp.
authors: Fumio
authors: F
authors: Matsuda
authors: Mass Spectrometry Business Unit, Shimadzu Corporation, 1, Nishinokyo Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan. Electronic address: ogutairo@shimadzu.co.jp.
authors: Tairo
authors: T
authors: Ogura
authors: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, Japan. Electronic address: atsumi_tomita@bio.eng.osaka-u.ac.jp.
authors: Atsumi
authors: A
authors: Tomita
authors: Mass Spectrometry Business Unit, Shimadzu Corporation, 1, Nishinokyo Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan. Electronic address: i_hirano@shimadzu.co.jp.
authors: Ichiro
authors: I
authors: Hirano
authors: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, Japan. Electronic address: shimizu@bio.eng.osaka-u.ac.jp.
authors: Hiroshi
authors: H
authors: Shimizu
mesh_terms: D002853:Chromatography, Liquid; D013058:Mass Spectrometry / Q000379:methods*; D053858:Metabolic Networks and Pathways; D010455:Peptides / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism
chemical_list: D010455:Peptides; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D014357:Trypsin
keywords: Central carbon metabolism; Enzyme expression; Quantitative proteomics; Saccharomyces cerevisiae; Ultra fast mass spectrometry