title: Expression of the porcine lipoic acid synthase (LIAS) gene in Escherichia coli.
abstract: Lipoic acid synthase, which exists primarily in mitochondria, participates in the biosynthesis of intrinsic lipoic acid. The lipoic acid synthase gene in pig is known as LIAS. To further investigate the biological functions of the protein that is encoded by LIAS, we cloned the open read frame of porcine LIAS (GenBank No. JN797612.1) into the expression vector pET-28α(+). The resulting pET-28α(+)-Lias recombinant vector was introduced into the Escherichia coli BL21 (DE3) strain. With induction by isopropyl β-D-1-thiogalactopyranoside, the recombinant E. coli strain can express the target protein that has a molecular weight of 41.58 kDa, which was confirmed by Western blotting.
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, China.
authors: W F
authors: WF
authors: Lu
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, China.
authors: J J
authors: JJ
authors: Cao
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, China.
authors: Y J
authors: YJ
authors: Guo
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, China.
authors: K
authors: K
authors: Zhong
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, China.
authors: G M
authors: GM
authors: Zha
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, China.
authors: L F
authors: LF
authors: Wang
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, China 5639400@qq.com.
authors: G Y
authors: GY
authors: Yang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008970:Molecular Weight; D016366:Open Reading Frames; D010957:Plasmids / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013466:Sulfurtransferases / Q000235:genetics* / Q000378:metabolism; D013552:Swine
chemical_list: D011994:Recombinant Proteins; D013466:Sulfurtransferases; C116435:lipoic acid synthase