title: Overview of genetic tools and techniques to study Notch signaling in mice.
abstract: Aberrations of Notch signaling in humans cause both congenital and acquired defects and cancers. Genetically engineered mice provide the most efficient and cost-effective models to study Notch signaling in a mammalian system. Here, we review the various types of genetic models, tools, and strategies to study Notch signaling in mice, and provide examples of their use. We also provide advice on breeding strategies for conditional mutant mice, and a protocol for tamoxifen administration to mouse strains expressing inducible Cre recombinase-estrogen receptor fusion proteins.
authors: Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME, 04074, USA, gridlt@mmc.org.
authors: Thomas
authors: T
authors: Gridley
authors: Andrew K
authors: AK
authors: Groves
mesh_terms: D000818:Animals; D001947:Breeding / Q000379:methods; D005821:Genetic Techniques; D019426:Integrases / Q000235:genetics; D051379:Mice / Q000235:genetics*; D009154:Mutation; D051880:Receptors, Notch / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015398:Signal Transduction; D013629:Tamoxifen / Q000008:administration & dosage
chemical_list: D051880:Receptors, Notch; D011993:Recombinant Fusion Proteins; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases