title: Expression and distribution of TNF-α and PGE2 of periodontal tissues in rat periodontitis model.
abstract: OBJECTIVE
  To simulate the expression of TNF-α and PGE2 of periodontal tissues in rat periodontitis model.
  
  
  METHODS
  40 Wistar rats were randomly divided into the periodontitis group and the control group (n=20). After the successful establishment of periodontitis rat model, raising for six weeks before the animals were sacrificed. The periodontal tissues were obtained and made into slices. Observed the histopathological changes of the periodontal tissues and measured TNF-α, PGE2 levels change by immunohistochemistry, Western blot analysis and ELISA.
  
  
  RESULTS
  TNF-α, PGE2 expression of the periodontitis group was significantly higher than that in the control group, the difference was significant (P<0.05).
  
  
  CONCLUSIONS
  The TNF-α, PGE2 expression of the rat periodontal tissue in the periodontitis group was significantly higher than the control group.
authors: Oral and Maxillofacial Surgery of Sichuan Provincial Academy of Medical Sciences, Sichuan Provincial People's Hospital 610072, Chengdu, China.
authors: Chu-Hang
authors: CH
authors: Liao
authors: Oral and Maxillofacial Surgery of Sichuan Provincial Academy of Medical Sciences, Sichuan Provincial People's Hospital 610072, Chengdu, China.
authors: Wei
authors: W
authors: Fei
authors: Oral and Maxillofacial Surgery of Sichuan Provincial Academy of Medical Sciences, Sichuan Provincial People's Hospital 610072, Chengdu, China.
authors: Zhi-Hao
authors: ZH
authors: Shen
authors: Oral and Maxillofacial Surgery of Sichuan Provincial Academy of Medical Sciences, Sichuan Provincial People's Hospital 610072, Chengdu, China.
authors: Ming-Ping
authors: MP
authors: Yin
authors: Chengdu Medical College 610083, Chengdu, China.
authors: Chen
authors: C
authors: Lu
mesh_terms: D000818:Animals; D015232:Dinoprostone / Q000032:analysis / Q000378:metabolism*; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D010518:Periodontitis / Q000378:metabolism*; D010519:Periodontium / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone
keywords: Periodontitis model; Prostaglandin E(2); Tumor necrosis factor-A