title: Tris buffer modulates polydopamine growth, aggregation, and paramagnetic properties.
abstract: Despite the growing technological interest of polydopamine (dopamine melanin)-based coatings for a broad variety of applications, the factors governing particle size, shape, and electronic properties of this bioinspired multifunctional material have remained little understood. Herein, we report a detailed characterization of polydopamine growth, particle morphology, and paramagnetic properties as a function of dopamine concentration and nature of the buffer (pH 8.5). Dynamic Light Scattering data revealed an increase in the hydrodynamic radii (Rh) of melanin particles with increasing dopamine concentration in all buffers examined, especially in phosphate buffer. Conversely, a marked inhibition of particle growth was apparent in Tris buffer, with Rh remaining as low as <100 nm during polymerization of 0.5 mM dopamine. Small angle neutron scattering data suggested formation of bidimensional structures in phosphate or bicarbonate buffers, while apparently three-dimensional fractal objects prevailed in Tris buffer. Finally, electron paramagnetic resonance spectra revealed a broader signal amplitude with a peculiar power saturation decay profile for polydopamine samples prepared in Tris buffer, denoting more homogeneous paramagnetic centers with respect to similar samples obtained in phosphate and bicarbonate buffers. Overall, these results disclose Tris buffer as an efficient modulator of polydopamine buildup and properties for the rational control and fine-tuning of melanin aggregate size, morphology, and free radical behavior.
authors: Dipartimento di Scienze Chimiche, Università degli Studi di Napoli "Federico II" , Via Cintia, 80126 Napoli, Italy.
authors: Nicola Fyodor
authors: NF
authors: Della Vecchia
authors: Alessandra
authors: A
authors: Luchini
authors: Alessandra
authors: A
authors: Napolitano
authors: Gerardino
authors: G
authors: D'Errico
authors: Giuseppe
authors: G
authors: Vitiello
authors: Noemi
authors: N
authors: Szekely
authors: Marco
authors: M
authors: d'Ischia
authors: Luigi
authors: L
authors: Paduano
mesh_terms: D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014325:Tromethamine / Q000737:chemistry*
chemical_list: D007211:Indoles; D011108:Polymers; C568283:polydopamine; D014325:Tromethamine