title: Random insertional-deletional strand exchange mutagenesis (RAISE): a simple method for generating random insertion and deletion mutations.
abstract: Although proteins can be artificially improved by random insertion and deletion mutagenesis methods, these procedures are technically difficult. Here we describe a simple method called random insertional-deletional strand exchange mutagenesis (RAISE). This method is based on gene shuffling and can be used to introduce a wide variety of insertions, deletions, and substitutions. RAISE involves three steps: DNA fragmentation, attachment of a random short sequence, and reconstruction. This yields unique mutants and can be a powerful technique for protein engineering.
authors: Synthetic Chemicals Laboratory, Mitsui Chemicals, Inc., 580-32 Nagaura, Sodegaura, Chiba, 299-0265, Japan.
authors: Ryota
authors: R
authors: Fujii
authors: Motomitsu
authors: M
authors: Kitaoka
authors: Kiyoshi
authors: K
authors: Hayashi
mesh_terms: D016254:Mutagenesis, Insertional / Q000379:methods*; D017384:Sequence Deletion / Q000235:genetics