title: Calorie restriction does not elicit a robust extension of replicative lifespan in Saccharomyces cerevisiae.
abstract: Calorie restriction (CR) is often described as the most robust manner to extend lifespan in a large variety of organisms. Hence, considerable research effort is directed toward understanding the mechanisms underlying CR, especially in the yeast Saccharomyces cerevisiae. However, the effect of CR on lifespan has never been systematically reviewed in this organism. Here, we performed a meta-analysis of replicative lifespan (RLS) data published in more than 40 different papers. Our analysis revealed that there is significant variation in the reported RLS data, which appears to be mainly due to the low number of cells analyzed per experiment. Furthermore, we found that the RLS measured at 2% (wt/vol) glucose in CR experiments is partly biased toward shorter lifespans compared with identical lifespan measurements from other studies. Excluding the 2% (wt/vol) glucose experiments from CR experiments, we determined that the average RLS of the yeast strains BY4741 and BY4742 is 25.9 buds at 2% (wt/vol) glucose and 30.2 buds under CR conditions. RLS measurements with a microfluidic dissection platform produced identical RLS data at 2% (wt/vol) glucose. However, CR conditions did not induce lifespan extension. As we excluded obvious methodological differences, such as temperature and medium, as causes, we conclude that subtle method-specific factors are crucial to induce lifespan extension under CR conditions in S. cerevisiae.
authors: Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG, Groningen, The Netherlands;
authors: Daphne H E W
authors: DH
authors: Huberts
authors: Probability and Statistics, Johann Bernoulli Institute of Mathematics and Computer Science, University of Groningen, 9747 AG, Groningen, The Netherlands; and.
authors: Javier
authors: J
authors: González
authors: Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland.
authors: Sung Sik
authors: SS
authors: Lee
authors: Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG, Groningen, The Netherlands;
authors: Athanasios
authors: A
authors: Litsios
authors: Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG, Groningen, The Netherlands;
authors: Georg
authors: G
authors: Hubmann
authors: Probability and Statistics, Johann Bernoulli Institute of Mathematics and Computer Science, University of Groningen, 9747 AG, Groningen, The Netherlands; and.
authors: Ernst C
authors: http://orcid.org/0000-0002-3671-9610
authors: EC
authors: Wit
authors: Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG, Groningen, The Netherlands; m.heinemann@rug.nl.
authors: Matthias
authors: M
authors: Heinemann
mesh_terms: D000818:Animals; D031204:Caloric Restriction; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D008136:Longevity / Q000502:physiology; D046210:Microfluidic Analytical Techniques; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013045:Species Specificity; D013997:Time Factors
chemical_list: D003470:Culture Media; D005947:Glucose