title: Inhibitory effects of low-dose aloe-emodin on the development of colorectal tumors in min mice.
abstract: Aloe-emodin (AE), a natural anthraquinone compound, has been reported to exhibit anticancer activity in various cancer cell lines and anti-inflammatory effects in murine macrophages. In the present study, we investigated the cancer chemopreventive effects of AE in an Apc-deficient Min mouse model. In the first experiment, male Min mice were fed a basal diet or diets containing 5 ppm AE and 10 ppm AE for 12 weeks. The dietary administration of 5 ppm AE significantly reduced the number of colorectal tumors. In a second experiment, we investigated the effects of AE on colitis-related colon carcinogenesis in Min mouse treated with dextran sodium sulfate (DSS). Female Min mice were administered 1% DSS in their drinking water for 7 days. AE was given to mice in their diet at a dose of 5 or 50 ppm for 5 weeks. Feeding with AE significantly reduced the number of colorectal tumors. When proliferation of cells in normal-appearing colonic mucosa was assessed by monoclonal anti-rat Ki-67 antibody (MIB-5) immunohistochemistry in experiments 1 and 2, the AE treatment significantly decreased the mean MIB-5-labeling index. These results suggest that the dietary administration of low-dose AE may have chemopreventive effects against development of colorectal tumors in Min mice, possibly in part by reducing cell proliferation in colorectal mucosa.
authors: Division of Biochemistry, Fujita Memorial Nanakuri Institute, Fujita Health University, Tsu, Japan E-mail : shimpo@fujita-hu.ac.jp.
authors: Kan
authors: K
authors: Shimpo
authors: Takeshi
authors: T
authors: Chihara
authors: Takaaki
authors: T
authors: Kaneko
authors: Hidehiko
authors: H
authors: Beppu
authors: Kazumasa
authors: K
authors: Wakamatsu
authors: Masanori
authors: M
authors: Shinzato
authors: Jun
authors: J
authors: Yukitake
authors: Shigeru
authors: S
authors: Sonoda
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D018890:Chemoprevention; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000517:prevention & control*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D016264:Dextran Sulfate; D019587:Dietary Supplements; D005260:Female; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D000880:Anthraquinones; D000970:Antineoplastic Agents; D008055:Lipids; D012333:RNA, Messenger; D016264:Dextran Sulfate; C518327:aloe emodin; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2