title: [Intraoperative methylene blue and (99m)Tc-sulfur colloid isotope tracing for sentinel node mapping in early-stage non-small cell lung cancer].
abstract: OBJECTIVE
  To compare the accuracy of intaoperative methylene blue alone and in combination with (99m)Tc-sulfur colloid isotopic tracing for detection of sentinel lymph nodes (SLNs) in early-stage non-small cell lung cancer (NSCLC).
  
  
  METHODS
  Sixty-one patients with operable NSCLC who did not receive previous radiotherapy or chemotherapy were enrolled. Methylene blue and (99m)Tc-sulfur colloid were injected into the subserosal layer adjacent to the tumor, and SLNs were defined as those with blue staining or those containing 3 times more radioactivity than the surrounding tissue detected with a gamma probe. The SLN were removed with systematic lymph node dissection. All the removed lymph nodes were examined histopathologically with HE staining and immunohistochemistry.
  
  
  RESULTS
  Methylene blue alone showed a low detection rate (60.0%) and sensitivity (58.33%) for SLNs compared with the combination of methylene blue and isotope tracing (96.15% and 92.86%, respectively).
  
  
  CONCLUSIONS
  The combination of methylene blue and (99m)Tc-sulfur colloid isotopic tracing allows accurate detection of the SLNs in early-stage NSCLC.
authors: Department of Cardiothoracic Surgery, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China. E-mail: 13996250323@139.com.
authors: Bin
authors: B
authors: Hong
authors: Xueyuan
authors: X
authors: Shen
authors: Jiangyong
authors: J
authors: Chen
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis*; D003102:Colloids; D006801:Humans; D007150:Immunohistochemistry; D007554:Isotopes; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000175:diagnosis; D008751:Methylene Blue; D021701:Sentinel Lymph Node Biopsy; D013455:Sulfur; D013671:Technetium Tc 99m Sulfur Colloid
chemical_list: D003102:Colloids; D007554:Isotopes; D013671:Technetium Tc 99m Sulfur Colloid; D013455:Sulfur; D008751:Methylene Blue