title: Purification and characterization of a cellulolytic multienzyme complex produced by Neocallimastix patriciarum J11.
abstract: Understanding the roles of the components of the multienzyme complex of the anaerobial cellulase system, acting on complex substrates, is crucial to the development of efficient cellulase systems for industrial applications such as converting lignocellulose to sugars for bioethanol production. In this study, we purified the multienzyme complex of Neocallimastix patriciarum J11 from a broth through cellulose affinity purification. The multienzyme complex is composed of at least 12 comprised proteins, based on sodium dodecyl sulfate polyacrylamide gel electrophoresis. Eight of these constituents have demonstrated β-glucanase activity on zymogram analysis. The multienzyme complex contained scaffoldings that respond to the gathering of the cellulolytic components. The levels and subunit ratio of the multienzyme complex from N. patriciarum J11 might have been affected by their utilized carbon sources, whereas the components of the complexes were consistent. The trypsin-digested peptides of six proteins were matched to the sequences of cellulases originating from rumen fungi, based on identification through liquid chromatography/mass spectrometry, revealing that at least three types of cellulase, including one endoglucanase and two exoglucanases, could be found in the multienzyme complex of N. patriciarum J11. The cellulolytic subunits could hydrolyze synergistically on both the internal bonds and the reducing and nonreducing ends of cellulose. Based on our research, our findings are the first to depict the composition of the multienzyme complex produced by N. patriciarum J11, and this complex is composed of scaffoldin and three types of cellulase.
authors: Department of Biochemical Science and Technology, National Taiwan University, Taipei, Taiwan.
authors: Hui-Chang
authors: HC
authors: Wang
authors: Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, Taiwan. Electronic address: ox@mail.npust.edu.tw.
authors: Yo-Chia
authors: YC
authors: Chen
authors: Department of Biochemical Science and Technology, National Taiwan University, Taipei, Taiwan. Electronic address: rshseu@ntu.edu.tw.
authors: Ruey-Shyang
authors: RS
authors: Hseu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002020:Buffaloes / Q000382:microbiology; D044602:Cellulases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D020088:Neocallimastix / Q000201:enzymology* / Q000302:isolation & purification; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012417:Rumen / Q000382:microbiology; D053719:Tandem Mass Spectrometry
chemical_list: D005656:Fungal Proteins; D009097:Multienzyme Complexes; D021122:Protein Subunits; D011994:Recombinant Proteins; D044602:Cellulases
keywords: Anaerobic fungi; Cellulosome; Multienzyme complex; Neocallimastix patriciarum; Renewable energy