title: Protecting capacity against malaria of chemically defined tetramer forms based on the Plasmodium falciparum apical sushi protein as potential vaccine components.
abstract: Developing novel generations of subunit-based antimalarial vaccines in the form of chemically-defined macromolecule systems for multiple antigen presentation represents a classical problem in the field of vaccine development. Many efforts involving synthesis strategies leading to macromolecule constructs have been based on dendrimer-like systems, the condensation of large building blocks and conventional asymmetric double dimer constructs, all based on lysine cores. This work describes novel symmetric double dimer and condensed linear constructs for presenting selected peptide multi-copies from the apical sushi protein expressed in Plasmodium falciparum. These molecules have been proved to be safe and innocuous, highly antigenic and have shown strong protective efficacy in rodents challenged with two Plasmodium species. Insights into systematic design, synthesis and characterisation have led to such novel antigen systems being used as potential platforms for developing new anti-malarial vaccine candidates.
authors: Fundación Instituto de Inmunología de Colombia (FIDIC) - Universidad del Rosario, Bogotá, Colombia; Universidad Nacional de Colombia, Bogotá, Colombia.
authors: Magnolia
authors: M
authors: Vanegas
authors: Fundación Instituto de Inmunología de Colombia (FIDIC) - Universidad del Rosario, Bogotá, Colombia.
authors: Adriana
authors: A
authors: Bermúdez
authors: Fundación Instituto de Inmunología de Colombia (FIDIC) - Universidad del Rosario, Bogotá, Colombia.
authors: Yuly Andrea
authors: YA
authors: Guerrero
authors: Universidad Nacional de Colombia, Bogotá, Colombia.
authors: Jesús Alfredo
authors: JA
authors: Cortes-Vecino
authors: Fundación Instituto de Inmunología de Colombia (FIDIC) - Universidad del Rosario, Bogotá, Colombia.
authors: Hernando
authors: H
authors: Curtidor
authors: Fundación Instituto de Inmunología de Colombia (FIDIC) - Universidad del Rosario, Bogotá, Colombia; Universidad Nacional de Colombia, Bogotá, Colombia.
authors: Manuel Elkin
authors: ME
authors: Patarroyo
authors: Fundación Instituto de Inmunología de Colombia (FIDIC) - Universidad del Rosario, Bogotá, Colombia; Universidad Nacional de Colombia, Bogotá, Colombia. Electronic address: jm_lozano@fidic.org.co.
authors: José Manuel
authors: JM
authors: Lozano
mesh_terms: D000595:Amino Acid Sequence; D000614:Aminocaproates / Q000737:chemistry; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D000939:Epitopes; D006801:Humans; D008288:Malaria / Q000517:prevention & control; D017780:Malaria Vaccines / Q000737:chemistry* / Q000494:pharmacology*; D016778:Malaria, Falciparum / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D010962:Plasmodium berghei / Q000472:pathogenicity; D010963:Plasmodium falciparum / Q000737:chemistry*; D010967:Plasmodium yoelii / Q000472:pathogenicity; D011487:Protein Conformation; D055503:Protein Multimerization; D011817:Rabbits; D022223:Vaccines, Subunit / Q000737:chemistry / Q000276:immunology
chemical_list: D000614:Aminocaproates; D000953:Antigens, Protozoan; D000939:Epitopes; D017780:Malaria Vaccines; D010455:Peptides; D022223:Vaccines, Subunit
keywords: Antimalarial-vaccine; Dendrimer; Macromolecule; PfASP; Plasmodium