title: Analysis of protein-protein interaction between late cornified envelope proteins and corneodesmosin.
abstract: Deletion of two members of the late cornified envelope (LCE) family, LCE3B and LCE3C (LCE3C_LCE3B-del), has been identified as risk factor for psoriasis with a possible role in skin barrier function. Moreover, genetic interaction between LCE3C_LCE3B-del and HLA-C*06, located in the psoriasis susceptibility regions 4 and 1 (PSORS4 and 1), has been reported in several populations. Because of high linkage disequilibrium between the PSORS1 genes HLA-C*06 and corneodesmosin (CDSN), both genes are potentially involved in psoriasis. As corneodesmosin and LCE proteins are both constituents of the stratum corneum, we investigated potential direct protein-protein interactions between six LCE proteins and two corneodesmosin sequence variants. Partial colocalization of LCE2 and CDSN was observed in normal and psoriasis skin using immunofluorescence microscopy. Co-expression of eCFP-LCE and mRFP-CDSN proteins in COS-1 cells and human adult keratinocytes, and GST pull-down results did not provide evidence for direct interactions between LCE proteins and CDSN variants.
authors: Department of Dermatology, Radboud University Medical Center, Nijmegen, The Netherlands.
authors: Judith G M
authors: JG
authors: Bergboer
authors: Maria G
authors: MG
authors: Dulak
authors: Ivonne M J J
authors: IM
authors: van Vlijmen-Willems
authors: Nathalie
authors: N
authors: Jonca
authors: Erwin
authors: E
authors: van Wijk
authors: Wiljan J A J
authors: WJ
authors: Hendriks
authors: Patrick L J M
authors: PL
authors: Zeeuwen
authors: Joost
authors: J
authors: Schalkwijk
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D055233:Cornified Envelope Proline-Rich Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014644:Genetic Variation; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D015603:Keratinocytes / Q000378:metabolism; D015810:Linkage Disequilibrium; D025941:Protein Interaction Mapping; D011565:Psoriasis / Q000235:genetics / Q000378:metabolism; D012307:Risk Factors; D012867:Skin / Q000378:metabolism
chemical_list: C109656:CDSN protein, human; D055233:Cornified Envelope Proline-Rich Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins
keywords: HLA-C; corneodesmosin; keratinocytes; late cornified envelope; psoriasis