title: Coating of nanoparticles on cryogel surface and subsequent double-modification for enhanced ion-exchange capacity of protein.
abstract: A novel composite cryogel monolith was developed by coating poly(glycidyl methacrylate) nanoparticles (NPs) onto the pore wall surface of poly(acrylamide) cryogel. The NPs-coated column was double-modified with poly(ethylenimine) (PEI) and diethylaminoethyl in sequence. Scanning electron microscopy revealed the dense coating of the NPs on the cryogel surface, but the NPs-coating did not result in distinct changes of the column porosity and permeability. The rough pore wall surface and extended polymer chains offered more binding sites, so the dynamic binding capacity of the composite cryogel bed for bovine serum albumin reached 11.7mg/mL bed volume at a flow rate of 6cm/min, which was 4.2 times higher than that of the cryogel bed modified with PEI without coating NPs (2.8mg/mL). The binding capacity as well as column efficiency decreased only slightly with increasing flow rate from 0.6 to 12cm/min. The results indicated that the strategy of NPs-coating incorporating with double ion-exchanger modifications is promising for enhancing cryogel capacities, and the novel material would be useful for high-speed protein chromatography.
authors: Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Shi-Peng
authors: SP
authors: Tao
authors: Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Chuan
authors: C
authors: Wang
authors: Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China. Electronic address: ysun@tju.edu.cn.
authors: Yan
authors: Y
authors: Sun
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D059985:Cryogels / Q000737:chemistry*; D007475:Ion Exchange Resins / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D059985:Cryogels; D007475:Ion Exchange Resins; D011108:Polymers; D012710:Serum Albumin, Bovine
keywords: Adsorption capacity; Column efficiency; Cryogel monolith; Double-modification; Nanoparticles-coating; Protein chromatography