title: A thiophene-modified screen printed electrode for detection of dengue virus NS1 protein.
abstract: A thiophene-modified screen printed electrode (SPE) for detection of the Dengue virus non-structural protein 1 (NS1), an important marker for acute phase diagnosis, is described. A sulfur-containing heterocyclic compound, the thiophene was incorporated to a carbon ink to prepare reproducible screen printed electrodes. After cured, the thiophene SPE was coated by gold nanoparticles conjugated to Protein A to form a nanostrutured surface. The Anti-NS1 antibodies immobilized via their Fc portions via Protein A, leaving their antigen specific sites free circumventing the problem of a random antibodies immobilization. Amperometric responses to the NS1 protein of dengue virus were obtained by cyclic voltammetries performed in presence of ferrocyanide/ferricyanide as redox probe. The calibration curve of immunosensor showed a linear response from 0.04 µg mL(-1) to 0.6 µg mL(-1) of NS1 with a good linear correlation (r=0.991, p<0.05). The detection limit (0.015 µg mL(-1) NS1) was lower than conventional analytical methods. In this work, thiophene monomers incorporated in the carbon ink enhanced the electroanalytical properties of the SPEs, increasing their reproducibility and sensitivity. This point-of-care testing represents a great potential for use in epidemic situations, facilitating the early diagnosis in acute phase of dengue virus.
authors: Biomedical Engineering Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, Brazil.
authors: M M S
authors: MM
authors: Silva
authors: Biomedical Engineering Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, Brazil.
authors: A C M S
authors: AC
authors: Dias
authors: Aggeu Magalhães Research Center, FIOCRUZ-PE, Recife, Brazil.
authors: M T
authors: MT
authors: Cordeiro
authors: Aggeu Magalhães Research Center, FIOCRUZ-PE, Recife, Brazil; Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA, USA.
authors: E
authors: E
authors: Marques
authors: Institute of Chemical and Biotechnology, Federal University of Alagoas, Maceió, Brazil.
authors: M O F
authors: MO
authors: Goulart
authors: Biomedical Engineering Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, Brazil. Electronic address: rosa.dutra@ufpe.br.
authors: R F
authors: RF
authors: Dutra
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002244:Carbon / Q000737:chemistry; D002460:Cell Line; D003716:Dengue Virus / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D005295:Ferrocyanides / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D007281:Ink; D053768:Metal Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013876:Thiophenes / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
chemical_list: D000906:Antibodies; D055910:Antibodies, Immobilized; D005292:Ferricyanides; D005295:Ferrocyanides; C082515:NS1 protein, dengue-1 virus; D013876:Thiophenes; D017361:Viral Nonstructural Proteins; C007931:hexacyanoferrate III; D002244:Carbon; D006046:Gold; C020354:hexacyanoferrate II
keywords: Dengue virus; Immunosensor; Screen printed electrode; Thiophene