title: Mitochondrial oxidative phosphorylation transcriptome alterations in human amyotrophic lateral sclerosis spinal cord and blood.
abstract: Origins of onset and progression of motor neurodegeneration in amyotrophic lateral sclerosis (ALS) are not clearly known, but may include impairment of mitochondrial bioenergetics. We used quantitative PCR approaches to analyze the mitochondrial oxidative phosphorylation (OXPHOS) transcriptomes of spinal cord tissue and peripheral blood mononuclear cells (PBMC) from persons with sporadic ALS compared with those without neurological disease. Expression measurements of 88 different nuclear (n) and mitochondrial (mt) DNA-encoded OXPHOS genes showed mtDNA-encoded respiratory gene expression was significantly decreased in ALS spinal cord by 78-84% (ANOVA p < 0.002). We observed the same phenomenon in freshly isolated PBMC from ALS patients (reduced 24-35%, ANOVA p < 0.001) and reproduced it in a human neural stem cell model treated with 2',3'-dideoxycytidine (ddC) (reduced 52-78%, ANOVA p < 0.001). nDNA-encoded OXPHOS genes showed heterogeneously and mostly decreased expression in ALS spinal cord tissue. In contrast, ALS PBMC and ddC-treated stem cells showed no significant change in expression of nDNA OXPHOS genes compared with controls. Genes related to mitochondrial biogenesis (PGC-1α, TFAM, ERRα, NRF1, NRF2 and POLG) were queried with inconclusive results. Here, we demonstrate there is a systemic decrease in mtDNA gene expression in ALS central and peripheral tissues that support pursuit of bioenergetic-enhancing therapies. We also identified a combined nDNA and mtDNA gene set (n = 26), downregulated in spinal cord tissue that may be useful as a biomarker in the development of cell-based ALS models.
authors: Parkinson's and Movement Disorders Center, Virginia Commonwealth University, P.O. Box 980312, Richmond, VA, 23298, USA, acladd@vcu.edu.
authors: Amy C
authors: AC
authors: Ladd
authors: Paula M
authors: PM
authors: Keeney
authors: Maria M
authors: MM
authors: Govind
authors: James P
authors: JP
authors: Bennett
mesh_terms: D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics* / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D050259:Genes, Mitochondrial; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000502:physiology*; D063269:Mitochondrial Turnover / Q000235:genetics; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism; D010085:Oxidative Phosphorylation; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D013116:Spinal Cord / Q000378:metabolism*; D014157:Transcription Factors / Q000502:physiology; D059467:Transcriptome; D016047:Zalcitabine / Q000494:pharmacology
chemical_list: D004272:DNA, Mitochondrial; D012333:RNA, Messenger; D014157:Transcription Factors; D016047:Zalcitabine