title: Phototherapy up-regulates dentin matrix proteins expression and synthesis by stem cells from human-exfoliated deciduous teeth.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effects of infrared LED (850nm) irradiation on dentin matrix proteins expression and synthesis by cultured stem cells from human exfoliated deciduous teeth (SHED).
  
  
  METHODS
  Near-exfoliation primary teeth were extracted (n=3), and SHED cultures were characterized by immunofluorescence using STRO-1, CD44, CD146, Nanog and OCT3/4 antibodies, before experimental protocol. The SHEDs were seeded (3×10(4) cells/cm(2)) with DMEM containing 10% FBS. After 24-h incubation, the culture medium was replaced by osteogenic differentiation medium, and the cells were irradiated with LED light at energy densities (EDs) of 0 (control), 2, or 4J/cm(2) (n=8). The irradiated SHEDs were then evaluated for alkaline phosphatase (ALP) activity, total protein (TP) production, and collagen synthesis (SIRCOL™ Assay), as well as ALP, collagen type I (Col I), dentin sialophosphoprotein (DSPP), and dentin matrix acidic phosphoprotein (DMP-1) gene expression (qPCR). Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (α=0.05).
  
  
  RESULTS
  Increased ALP activity and collagen synthesis, as well as gene expression of DSPP and ALP, were observed for both EDs compared with non-irradiated cells. The ED of 4J/cm(2) also increased gene expression of COL I and DMP-1.
  
  
  CONCLUSIONS
  In conclusion, infrared LED irradiation was capable of biostimulating SHEDs by increasing the expression and synthesis of proteins related with mineralized tissue formation, with overall better results for the energy dose of 4J/cm(2).
  
  
  CONCLUSIONS
  Phototherapy is an additional approach for the clinical application of LED in Restorative Dentistry. Infrared LED irradiation of the cavity's floor could biostimulate subjacent pulp cells, improving local tissue healing.
authors: Department of Pediatric Dentistry and Orthodontics, UNESP-Univ. Estadual Paulista, School of Dentistry, Araraquara 14801903, SP, Brazil.
authors: Ana Paula S
authors: AP
authors: Turrioni
authors: Department of Physiology and Pathology, UNESP-Univ. Estadual Paulista, School of Dentistry, Araraquara 14801903, SP, Brazil.
authors: Fernanda G
authors: FG
authors: Basso
authors: Department of Pediatric Dentistry and Orthodontics, UNESP-Univ. Estadual Paulista, School of Dentistry, Araraquara 14801903, SP, Brazil.
authors: Liege A
authors: LA
authors: Montoro
authors: Department of Restorative Dentistry, UNESP-Univ. Estadual Paulista, School of Dentistry, Araraquara 14801903, SP, Brazil.
authors: Leopoldina de Fátima D de
authors: Lde F
authors: Almeida
authors: Department of Physiology and Pathology, UNESP-Univ. Estadual Paulista, School of Dentistry, Araraquara 14801903, SP, Brazil.
authors: Carlos A de Souza
authors: CA
authors: Costa
authors: Department of Pediatric Dentistry and Orthodontics, UNESP-Univ. Estadual Paulista, School of Dentistry, Araraquara 14801903, SP, Brazil. Electronic address: jhebling@foar.unesp.br.
authors: Josimeri
authors: J
authors: Hebling
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000528:radiation effects; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D024042:Collagen Type I / Q000032:analysis / Q000528:radiation effects; D003470:Culture Media; D003782:Dental Pulp / Q000166:cytology*; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000528:radiation effects*; D006801:Humans; D007259:Infrared Rays; D010012:Osteogenesis / Q000528:radiation effects; D010750:Phosphoproteins / Q000032:analysis / Q000528:radiation effects; D010789:Phototherapy / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000528:radiation effects; D011829:Radiation Dosage; D012795:Sialoglycoproteins / Q000032:analysis / Q000528:radiation effects; D013234:Stem Cells / Q000528:radiation effects*; D014080:Tooth Exfoliation; D014094:Tooth, Deciduous / Q000166:cytology*; D015854:Up-Regulation; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D024042:Collagen Type I; D003470:Culture Media; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011506:Proteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human
keywords: Dentin; Odontoblasts; Phototherapy; Protein expression; Pulp biology; Stem cells