title: Generating mouse lines for lineage tracing and knockout studies.
abstract: In 2007 Capecchi, Evans, and Smithies received the Nobel Prize in recognition for discovering the principles for introducing specific gene modifications in mice via embryonic stem cells, a technology, which has revolutionized the field of biomedical science allowing for the generation of genetically engineered animals. Here we describe detailed protocols based on and developed from these ground-breaking discoveries, allowing for the modification of genes not only to create mutations to study gene function but additionally to modify genes with fluorescent markers, thus permitting the isolation of specific rare wild-type and mutant cell types for further detailed analysis at the biochemical, pathological, and genomic levels.
authors: Department of Biology, Clarkson University, Potsdam, 13699-5808, USA.
authors: Petra
authors: P
authors: Kraus
authors: V
authors: V
authors: Sivakamasundari
authors: Xing
authors: X
authors: Xing
authors: Thomas
authors: T
authors: Lufkin
mesh_terms: D000483:Alleles; D000818:Animals; D015139:Blotting, Southern; D018929:Cell Culture Techniques; D019070:Cell Lineage / Q000235:genetics*; D058948:Cell Tracking / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D018274:Electroporation; D010064:Embryo Implantation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005187:Fallopian Tubes / Q000502:physiology; D005260:Female; D055786:Gene Knockout Techniques / Q000379:methods*; D018390:Gene Targeting / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics; D051379:Mice; D008845:Microinjections; D013997:Time Factors; D020858:Tissue and Organ Harvesting
chemical_list: D004247:DNA