title: Prognostic value of miR-221-3p, miR-342-3p and miR-491-5p expression in colon cancer.
abstract: Increasing evidence has demonstrated that microRNAs (miRNAs) are involved in colon cancer initiation and progression, and may serve as diagnostic and prognostic biomarkers for colon cancer. Here, we investigated the levels of miR-9-1, miR-203-3p, miR-221-3p, miR-342-3p, miR-491-5p and miR-503-5p in 90 pairs of colon cancer and adjacent normal tissues, and explored the relationship between their expression and clinical outcome of colon cancer. Five miRNAs (miR-203-3p, miR-221-3p, miR-342-3p, miR-491-5p and miR-503-5p) were dysregulated in colon cancer tissue (P < 0.05). The levels of miR-503-5p in larger tumors (≥ 6 cm) were higher than those in smaller ones (< 6 cm) (P = 0.031), while the levels of miR-203-3p and miR-491-5p in patients aged 70 years and older were higher than those in patients aged younger than 70 years (P = 0.019 and 0.049, respectively). The high levels of miR-221-3p (HR = 2.416, 95% CI 1.314-4.445, P = 0.005), miR-342-3p (HR = 1.807, 95% CI 1.003-3.253, P = 0.049) and miR-491-5p (HR = 1.868, 95% CI 1.032-3.384, P = 0.039) were significantly associated with worse survival time. Moreover, combination analysis of miR-221-3p, miR-342-3p and miR-491-5p expression revealed that patients with 3 highly expressed miRNAs had lower survival rates compared with those with zero-to-two highly expressed miRNAs (HR = 2.100, 95% CI 1.157-3.813, P = 0.015), especially those with TNM stages I and II (HR = 4.204,95% CI 1.762-10.030, P = 0.001). Our results suggest that the three-miRNA signature may help doctors better predict prognosis and guide treatment decisions for colon cancer.
authors: Department of Pathology, Tongren Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China ; Department of Pathology, Changzheng Hospital, Second Military Medical University Shanghai, China.
authors: Kun
authors: K
authors: Tao
authors: Department of Pathology, Tongren Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors: Jing
authors: J
authors: Yang
authors: Department of Pathology, Tongren Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors: Zhenhua
authors: Z
authors: Guo
authors: Department of Pathology, Tongren Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors: Yuemei
authors: Y
authors: Hu
authors: Institute of CMC Biobank and Translational Medicine Taizhou, Jiangsu, China ; Shanghai Engineering Center for Molecular Medicine, National Engineering Center for Biochip at Shanghai Shanghai, China ; Department of Gastroenterology, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine Shanghai, China.
authors: Haihui
authors: H
authors: Sheng
authors: Institute of CMC Biobank and Translational Medicine Taizhou, Jiangsu, China ; Shanghai Engineering Center for Molecular Medicine, National Engineering Center for Biochip at Shanghai Shanghai, China ; Department of Gastroenterology, Tongji Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine Shanghai, China.
authors: Hengjun
authors: H
authors: Gao
authors: Department of Pathology, Changzheng Hospital, Second Military Medical University Shanghai, China.
authors: Hongyu
authors: H
authors: Yu
keywords: colon cancer; miR-221-3p; miR-342-3p; miR-491-5p; prognosis