title: Peripheral blood mononuclear cells as in vivo model for dietary intervention induced systemic oxidative stress.
abstract: Our aim was to assess the use of peripheral blood mononuclear cells (PBMC) as an in vivo cellular model to evaluate diet-induced changes in the oxidative stress status by analyzing the gene expression pattern of NADPH-oxidase subunits and antioxidant genes. A randomized, controlled trial assigned metabolic syndrome patients to 4 diets for 12 weeks each: (i) high-saturated fatty acid (HSFA), (ii) high-monounsaturated fatty acid, and (iii), (iv) two low-fat, high-complex carbohydrate diets supplemented with n-3 polyunsaturated fatty acids or placebo. A fat challenge reflecting the fatty acid composition as the original diets was conducted post-intervention. The mRNA levels of gp91(phox) (P<0.001), p22(phox) (P=0.005), p47(phox) (P=0.001) and p40(phox) (P<0.001) increased at 2h after the intake of the HSFA meal. The expression of SOD1, SOD2, GSR, GPx1, GPX4, TXN, TXNRD1 and Nrf2 increased after the HSFA meal (p<0.05). In contrast, the expression of these genes remained unaltered in response to the other dietary interventions. Our results suggest that the increased expression of antioxidant genes in PBMC seems to be due to the response to the postprandial oxidative stress generated mainly in adipose tissue after the consumption of an HSFA diet.
authors: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain.
authors: Antonio
authors: A
authors: Camargo
authors: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain.
authors: Patricia
authors: P
authors: Peña-Orihuela
authors: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain.
authors: Oriol Alberto
authors: OA
authors: Rangel-Zúñiga
authors: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain.
authors: Pablo
authors: P
authors: Pérez-Martínez
authors: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain.
authors: Javier
authors: J
authors: Delgado-Lista
authors: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain.
authors: Cristina
authors: C
authors: Cruz-Teno
authors: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain.
authors: Carmen
authors: C
authors: Marín
authors: CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain; Endocrinology and Nutrition Service, Hospital Virgen de la Victoria, Málaga, Spain.
authors: Francisco
authors: F
authors: Tinahones
authors: CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain; Department of Cell Biology, Physiology, and Immunology, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Cordoba, Spain.
authors: María M
authors: MM
authors: Malagón
authors: UCD Institute of Food & Health/UCD Conway Institute, School of Public Health and Population Sciences, University College Dublin, Ireland.
authors: Helen M
authors: HM
authors: Roche
authors: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain.
authors: Francisco
authors: F
authors: Pérez-Jiménez
authors: Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital, University of Cordoba, Cordoba, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain. Electronic address: jlopezmir@uco.es.
authors: José
authors: J
authors: López-Miranda
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000494:pharmacology; D059305:Diet, High-Fat; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D019587:Dietary Supplements; D005227:Fatty Acids / Q000008:administration & dosage; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D005260:Female; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D024821:Metabolic Syndrome / Q000178:diet therapy / Q000235:genetics; D008875:Middle Aged; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D019518:Postprandial Period / Q000187:drug effects; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D054481:Thioredoxin Reductase 1 / Q000235:genetics / Q000378:metabolism; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
chemical_list: D000975:Antioxidants; D004040:Dietary Carbohydrates; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D015525:Fatty Acids, Omega-3; D005979:Glutathione Peroxidase; D019255:NADPH Oxidases; D051267:NF-E2-Related Factor 2; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D054481:Thioredoxin Reductase 1; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2; C495635:NFE2L2 protein, human; C000606290:SOD1 protein, human; C516124:TXNRD1 protein, human; C000722067:GPX1 protein, human
keywords: Diet; LIPGENE study; Metabolic syndrome; Mononuclear cells; Oxidative stress; Postprandial state