title: Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.
abstract: In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions. Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold. In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01. Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment. Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation. Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus.
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Ming
authors: M
authors: Li
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Shengli
authors: S
authors: Si
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Bo
authors: B
authors: Hao
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Yi
authors: Y
authors: Zha
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Can
authors: C
authors: Wan
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Shufen
authors: S
authors: Hong
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Yongbo
authors: Y
authors: Kang
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Jun
authors: J
authors: Jia
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Jing
authors: J
authors: Zhang
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Meng
authors: M
authors: Li
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Chunqiao
authors: C
authors: Zhao
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Yuanyuan
authors: Y
authors: Tu
authors: Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; Hubei Aohua Bioenergy Industrial Corporation Ltd., Hanchuan 431602, PR China.
authors: Shiguang
authors: S
authors: Zhou
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, PR China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, PR China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China; Hubei Aohua Bioenergy Industrial Corporation Ltd., Hanchuan 431602, PR China. Electronic address: lpeng@mail.hzau.edu.cn.
authors: Liangcai
authors: L
authors: Peng
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000191:economics / Q000379:methods*; D003362:Cost-Benefit Analysis; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects*; D006138:Guaiac / Q000302:isolation & purification*; D008031:Lignin / Q000302:isolation & purification*; D006109:Poaceae / Q000187:drug effects / Q000378:metabolism*; D011134:Polysaccharides / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D012972:Sodium Hydroxide / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D011134:Polysaccharides; C036909:lignocellulose; D000431:Ethanol; D012972:Sodium Hydroxide; C007916:hemicellulose; D006138:Guaiac; D008031:Lignin
keywords: Biomass digestibility; Fermentation inhibitor; Mild alkali pretreatment; Miscanthus; Monolignins