title: Expression analysis of self-incompatibility-associated genes in non-heading Chinese cabbage.
abstract: In Brassicaceae, a self-incompatibility (SI) system mediates pollen-pistil interactions. Self-pollen could be recognized and rejected by incompatible pistils. Several components involved in the SI response have been determined, including S-locus receptor kinase (SRK), S-locus cysteine-rich protein/S-locus protein 11, and arm repeat-containing protein 1 (ARC1). However, the components involved in the SI system of Brassicaceae are not fully understood. Here, we detected expression patterns of 24 SI-related genes in non-heading Chinese cabbage (Brassica campestris ssp chinensis Makino) after compatible and incompatible pollination, and potential interaction relationships of these genes were predicted. SRK and ARC1 transcripts increased initially 0.25 h after incompatible pollination, while kinase-associated protein phosphatase had an expression pattern that was opposite that of SRK transcripts during self-pollination. Plant U-box 8 was not required in the SI response of non-heading Chinese cabbage. Our results showed that the SI signal of non-heading Chinese cabbage could occur within 0.25 h after self-pollination. The hypothetical interaction relationships indicated that plastid-lipid-associated protein and malate dehydrogenase could be negatively regulated by chaperonin 10, glutathione transferase, cytidylate kinase/uridylate kinase, and methionine synthase by indirect interactions. Our findings could be helpful to better understand potential roles of these components in the SI system of non-heading Chinese cabbage.
authors: Horticultural Department, Nanjing Agricultural University, Nanjing, China.
authors: L
authors: L
authors: Wang
authors: Horticultural Department, Nanjing Agricultural University, Nanjing, China.
authors: C
authors: C
authors: Wang
authors: Horticultural Department, Nanjing Agricultural University, Nanjing, China.
authors: T T
authors: TT
authors: Ge
authors: Horticultural Department, Nanjing Agricultural University, Nanjing, China.
authors: J J
authors: JJ
authors: Wang
authors: Horticultural Department, Nanjing Agricultural University, Nanjing, China.
authors: T K
authors: TK
authors: Liu
authors: Horticultural Department, Nanjing Agricultural University, Nanjing, China.
authors: X L
authors: XL
authors: Hou
authors: yingli@njau.edu.cn.
authors: Y
authors: Y
authors: Li
mesh_terms: D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000235:genetics / Q000378:metabolism; D001937:Brassica / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018835:Chaperonin 10 / Q000235:genetics / Q000378:metabolism; D035264:Flowers / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism; D009703:Nucleoside-Phosphate Kinase / Q000235:genetics / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011058:Pollen / Q000235:genetics; D054817:Pollination / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D018835:Chaperonin 10; D006023:Glycoproteins; D010940:Plant Proteins; D012333:RNA, Messenger; C064156:self-incompatibility locus specific glycoprotein, Brassica; D008291:Malate Dehydrogenase; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D005982:Glutathione Transferase; D011494:Protein Kinases; C079832:S-receptor kinase; C019979:cytidylate kinase; D009703:Nucleoside-Phosphate Kinase; D010749:Phosphoprotein Phosphatases; D008666:Metalloendopeptidases