title: Expression of apical junction complex proteins in duodenal mucosa of dogs with inflammatory bowel disease.
abstract: OBJECTIVE
  To determine the expression of tight junction and adherens junction proteins in duodenal mucosa samples of dogs with inflammatory bowel disease (IBD).
  
  
  METHODS
  12 dogs with IBD and 6 healthy control Beagles.
  
  
  METHODS
  Duodenal mucosa biopsy samples were endoscopically obtained from dogs with IBD and healthy control Beagles. The expression of claudin-1, -2, -3, -4, -5, -7, and -8; E-cadherin; and β-catenin in the duodenal mucosa samples was determined by means of immunoblotting. The subcellular localization of E-cadherin in the duodenal mucosa samples was determined with immunofluorescence microscopy.
  
  
  RESULTS
  The expression of each claudin and β-catenin was not significantly different between control dogs and dogs with IBD. However, expression of E-cadherin was significantly lower in duodenal mucosa samples of dogs with IBD than it was in samples obtained from healthy control dogs. Results of immunofluorescence microscopy indicated decreased intensity of E-cadherin labeling in the tips of villi in duodenal mucosa samples obtained from 6 dogs with IBD, compared with staining intensity for other dogs.
  
  
  CONCLUSIONS
  Results of this study indicated expression of claudin-1, -2, -3, -4, -5, -7, and -8 and β-catenin was not significantly different between duodenal mucosa samples obtained from control dogs and those obtained from dogs with IBD. However, E-cadherin expression was significantly lower in the villus epithelium in duodenal mucosa samples obtained from dogs with IBD versus samples obtained from control dogs, which suggested that decreased expression of that protein has a role in the pathogenesis of IBD in dogs.
authors: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.
authors: Hiroshi
authors: H
authors: Ohta
authors: Yuji
authors: Y
authors: Sunden
authors: Nozomu
authors: N
authors: Yokoyama
authors: Tatsuyuki
authors: T
authors: Osuga
authors: Sue Yee
authors: SY
authors: Lim
authors: Yu
authors: Y
authors: Tamura
authors: Keitaro
authors: K
authors: Morishita
authors: Kensuke
authors: K
authors: Nakamura
authors: Masahiro
authors: M
authors: Yamasaki
authors: Mitsuyoshi
authors: M
authors: Takiguchi
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D016022:Case-Control Studies; D057167:Claudins / Q000378:metabolism; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D004386:Duodenum / Q000378:metabolism*; D015151:Immunoblotting / Q000662:veterinary; D015212:Inflammatory Bowel Diseases / Q000378:metabolism / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000378:metabolism*; D062726:Junctional Adhesion Molecules / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000662:veterinary; D018709:Statistics, Nonparametric; D062725:Tight Junction Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D057167:Claudins; D062726:Junctional Adhesion Molecules; D062725:Tight Junction Proteins; D051176:beta Catenin