title: MicroRNA-146a enhances Helicobacter pylori induced cell apoptosis in human gastric cancer epithelial cells.
abstract: Helicobacter pylori (H. pylori) infection induces apoptosis in gastric epithelial cells, and this occurrence may link to gastric carcinogenesis. However, the regulatory mechanism of H. pylori-induced apoptosis is not clear. MicroRNA-146a has been implicated as a key regulator of the immune system. This report describes our discovery of molecular mechanisms of microRNA-146a regulation of apoptosis in human gastric cancer cells. We found that overexpression of microRNA-146a by transfecting microRNA-146a mimics could significantly enhance apoptosis, and this up-regulation was triggered by COX-2 inhibition. Furthermore, we found that microRNA-146a density was positively correlated with apoptosis rates in H. pylori-positive gastric cancer tissues and intratumoral microRNA-146a density was negatively correlated with lymph node metastasis among H. pylori-positive gastric cancer patients. Understanding the important roles of microRNA-146a in regulating cell apoptosis in H. pylori infected human gastric cancer cells will contribute to the development of microRNA targeted therapy in the future.
authors: Department of Gastroenterology, 309 Hospital of Chinese People's Liberation Army, Beijing, China E-mail : jiayujiepaper@163.com.
authors: Kai
authors: K
authors: Wu
authors: Liu
authors: L
authors: Yang
authors: Cong
authors: C
authors: Li
authors: Chao-Hui
authors: CH
authors: Zhu
authors: Xin
authors: X
authors: Wang
authors: Yi
authors: Y
authors: Yao
authors: Yu-Jie
authors: YJ
authors: Jia
mesh_terms: D017209:Apoptosis / Q000235:genetics* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000473:pathology*; D016480:Helicobacter pylori; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics / Q000382:microbiology / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human