title: Platelet Reactivity in Patients With Stroke and Hyperlipidemia, GPIbα Assessment.
abstract: The aim of this study was to assess platelet reactivity in patients after ischemic stroke and to investigate the influence of hyperlipidemia (HL) on platelet activity markers. A total of 41 patients after ischemic stroke were divided into the following 2 groups: patients with HL and patients with normolipidemia. Expression of CD42b on resting, thrombin-activated blood platelets, and fibrinogen level was assessed. The CD42b-positive platelets were analyzed using the flow cytometer, anti-CD61, and anti-CD42b monoclonal antibodies. The results confirmed increased platelet reactivity to thrombin in all patients after ischemic stroke manifested by significantly lower CD42b expression and percentage of CD42b(+) platelets after activation by thrombin. The influence of HL on the expression of CD42b on resting and thrombin-activated platelets was not found. However, increased level of fibrinogen but no influence of HL on fibrinogen concentration was observed in patients after ischemic stroke. Increased susceptibility to platelet agonists was found in patients after ischemic stroke in the convalescent phase.
authors: Department of Neurology and Strokes, Medical University of Lodz, Lodz, Poland malgorzata.pawelczyk@umed.lodz.pl.
authors: Malgorzata
authors: M
authors: Pawelczyk
authors: Department of Neurology and Strokes, Medical University of Lodz, Lodz, Poland.
authors: Henryk
authors: H
authors: Chmielewski
authors: Department of Neurology and Strokes, Medical University of Lodz, Lodz, Poland.
authors: Beata
authors: B
authors: Kaczorowska
authors: Department of Neurology and Strokes, Medical University of Lodz, Lodz, Poland.
authors: Monika
authors: M
authors: Przybyła
authors: Department of Pathophysiology and Clinical Immunology, Medical University of Lodz, Lodz, Poland.
authors: Zbigniew
authors: Z
authors: Baj
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001792:Blood Platelets / Q000378:metabolism* / Q000473:pathology; D002545:Brain Ischemia / Q000097:blood* / Q000473:pathology; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D005500:Follow-Up Studies; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood* / Q000473:pathology; D039661:Integrin beta3 / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000096:biosynthesis*; D020521:Stroke / Q000097:blood* / Q000473:pathology; D013917:Thrombin / Q000494:pharmacology
chemical_list: C061248:ITGB3 protein, human; D039661:Integrin beta3; D019038:Platelet Glycoprotein GPIb-IX Complex; C094990:adhesion receptor; D005340:Fibrinogen; D013917:Thrombin
keywords: GPIbα; hyperlipidemia; ischemic stroke; platelet reactivity