title: Lipopolysaccharide and β-1,3-glucan binding protein in the hard clam (Meretrix meretrix): molecular characterization and expression analysis.
abstract: Pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. In this study, a lipopolysaccharide and β-1,3-glucan binding protein (LGBP) gene was cloned from the hard clam Meretrix meretrix (designated as Mm-LGBP) by the expressed sequence tags and rapid amplification of cDNA ends method. The cDNA was 1827 bp in length, consisting of a 71-bp 5'-terminal untranslated region, a 62-bp 3'UTR, and a 1734-bp open reading frame encoding a 577-amino acid polypeptide with an estimated molecular mass of 60.7 kDa and a theoretical isoelectric point of 5.56. Characteristic potential polysaccharide binding, cell adhesion, and glucanase motifs were identified in the Mm-LGBP, indicating that Mm-LGBP should be a new member of the LGBP family. Quantitative real-time polymerase chain reaction was developed to detect the mRNA expression level of Mm-LGBP in 6 different tissues. Higher-level mRNA expression of Mm-LGBP was detected in the gill and digestive gland tissues. The upregulation of Mm-LGBP mRNA after Vibrio anguillarum challenge showed that Mm-LGBP play a pivotal role in antibacterial immunity.
authors: Key Laboratory for Ecological Environment in Coastal Areas, State Oceanic Administration, National Marine Environmental Monitoring Center, Dalian, China.
authors: S X
authors: SX
authors: Liu
authors: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
authors: Z H
authors: ZH
authors: Qi
authors: Key Laboratory for Ecological Environment in Coastal Areas, State Oceanic Administration, National Marine Environmental Monitoring Center, Dalian, China.
authors: J J
authors: JJ
authors: Zhang
authors: Key Laboratory of Marine Fishery Molecular Biology of Liaoning Province, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors: C B
authors: CB
authors: He
authors: Key Laboratory of Marine Fishery Molecular Biology of Liaoning Province, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors: X G
authors: XG
authors: Gao
authors: Key Laboratory for Ecological Environment in Coastal Areas, State Oceanic Administration, National Marine Environmental Monitoring Center, Dalian, China.
authors: H J
authors: HJ
authors: Li
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D020816:Amino Acid Motifs; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010802:Phylogeny; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014733:Vibrio / Q000472:pathogenicity / Q000502:physiology; D047071:beta-Glucans / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D037102:Lectins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D047071:beta-Glucans; C033363:beta-1,3-glucan