title: Elevated transforming growth factor β1 in plasma of primary open-angle glaucoma patients.
abstract: OBJECTIVE
  To test the hypothesis that primary open-angle glaucoma (POAG) patients have a systemic elevation of transforming growth factor β1 (TGFβ1).
  
  
  METHODS
  Plasma was prepared from blood samples drawn from patients of the Vanderbilt Eye Institute during clinic visits. Concentrations of total TGFβ1 and thrombospondin-1 (TSP1) in plasma were determined by ELISA. Statistical significance of differences between POAG and control samples was evaluated by Mann-Whitney test. Regression analysis was used to evaluate correlations between plasma TGFβ1 and patient age and between plasma TGFβ1 and TSP1.
  
  
  RESULTS
  Plasma samples were obtained from 148 POAG patients and 150 controls. Concentration of total TGFβ1 in the plasma of POAG patients (median = 3.25 ng/mL) was significantly higher (P < 0.0001) than in controls (median = 2.46 ng/mL). Plasma TGFβ1 was not correlated with age of patient (P = 0.17). Thrombospondin-1 concentration was also significantly higher (P < 0.0001) in POAG patients (median = 0.774 μg/mL) as compared to controls (median = 0.567 μg/mL). Plasma total TGFβ1 and TSP1 concentrations were linearly correlated (P < 0.0001).
  
  
  CONCLUSIONS
  Plasma samples from POAG patients display elevated total TGFβ1 compared to controls, consistent with elevated systemic TGFβ1 in POAG patients.
authors: Vanderbilt Eye Institute, Vanderbilt University, Nashville, Tennessee, United States.
authors: John
authors: J
authors: Kuchtey
authors: Vanderbilt Eye Institute, Vanderbilt University, Nashville, Tennessee, United States.
authors: Jessica
authors: J
authors: Kunkel
authors: Vanderbilt Eye Institute, Vanderbilt University, Nashville, Tennessee, United States.
authors: L Goodwin
authors: LG
authors: Burgess
authors: Vanderbilt Eye Institute, Vanderbilt University, Nashville, Tennessee, United States.
authors: Megan B
authors: MB
authors: Parks
authors: Vanderbilt Eye Institute, Vanderbilt University, Nashville, Tennessee, United States.
authors: Milam A
authors: MA
authors: Brantley
authors: Vanderbilt Eye Institute, Vanderbilt University, Nashville, Tennessee, United States.
authors: Rachel W
authors: RW
authors: Kuchtey
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005902:Glaucoma, Open-Angle / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis; D019700:Thrombospondin 1 / Q000097:blood; D053773:Transforming Growth Factor beta1 / Q000097:blood*
chemical_list: D019700:Thrombospondin 1; D053773:Transforming Growth Factor beta1
keywords: aqueous humor; glaucoma; plasma; platelet; transforming growth factor beta