title: Analyses of flooding tolerance of soybean varieties at emergence and varietal differences in their proteomes.
abstract: Flooding of fields due to heavy and/or continuous rainfall influences soybean production. To identify soybean varieties with flooding tolerance at the seedling emergence stage, 128 soybean varieties were evaluated using a flooding tolerance index, which is based on plant survival rates, the lack of apparent damage and lateral root development, and post-flooding radicle elongation rate. The soybean varieties were ranked according to their flooding tolerance index, and it was found that the tolerance levels of soybean varieties exhibit a continuum of differences between varieties. Subsequently, tolerant, moderately tolerant and sensitive varieties were selected and subjected to comparative proteomic analysis to clarify the tolerance mechanism. Proteomic analysis of the radicles, combined with correlation analysis, showed that the ratios of RNA binding/processing related proteins and flooding stress indicator proteins were significantly correlated with flooding tolerance index. The RNA binding/processing related proteins were positively correlated in untreated soybeans, whereas flooding stress indicator proteins were negatively correlated in flooded soybeans. These results suggest that flooding tolerance is regulated by mechanisms through multiple factors and is associated with abundance levels of the identified proteins.
authors: NARO Institute of Crop Science, Tsukuba 305-8518, Japan. Electronic address: ynanjo@affrc.go.jp.
authors: Yohei
authors: Y
authors: Nanjo
authors: Chungbuk National University, Cheong-ju 361-763, Republic of Korea.
authors: Hee-Young
authors: HY
authors: Jang
authors: Chungbuk National University, Cheong-ju 361-763, Republic of Korea.
authors: Hong-Sig
authors: HS
authors: Kim
authors: NARO Institute of Crop Science, Tsukuba 305-8518, Japan.
authors: Susumu
authors: S
authors: Hiraga
authors: Chungbuk National University, Cheong-ju 361-763, Republic of Korea.
authors: Sun-Hee
authors: SH
authors: Woo
authors: NARO Institute of Crop Science, Tsukuba 305-8518, Japan. Electronic address: skomatsu@affrc.go.jp.
authors: Setsuko
authors: S
authors: Komatsu
mesh_terms: D055868:Floods; D010940:Plant Proteins / Q000502:physiology*; D020543:Proteome / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000502:physiology; D013025:Glycine max / Q000145:classification / Q000502:physiology*; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D020543:Proteome; D016601:RNA-Binding Proteins
keywords: Fabaceae; Flooding tolerance; Glycine gracilis; Glycine max; Proteomics; Soybean; Varietal difference