title: Heparin modulates the expression of genes encoding pro and anti-apoptotic proteins in endothelial cells exposed to intestinal ischemia and reperfusion in rats.
abstract: OBJECTIVE
  To investigate if expression of genes encoding pro and anti-apoptotic proteins in the rat enteric endothelial cells stimulated by intestinal ischemia followed by reperfusion (IR) can be modified by treatment with heparin (HP).
  
  
  METHODS
  Eighteen adult Wistar rats were divided in three groups: sham group submitted to laparotomy only (SG), ischemia followed by reperfusion group (IRG); ischemia followed by reperfusion plus pretreatment with HP 100 mg.kg-1 (IRG+HP). Ischemia was performed by clamping of the superior mesenteric artery. After 60 min of ischemia, metal clamps were removed for reperfusion for 120 min. Gene expression of encoding pro (Casp1, Casp6, Casp3, Cflar, Fas and Pgl) and anti-apoptotic (Bcl2, Bcl2l1 and Naip2) proteins in rat enteric endothelial cells was evaluated by PCR microarray method.
  
  
  RESULTS
  Compared to rat endothelial cells of SG, the expression of pro-apoptotic genes was up-regulated in IRG while anti-apoptotic genes were down-regulated. In contrast, the expression of anti-apoptotic genes in IRG+HP was up-regulated while pro-apoptotic genes was down-regulated compared to SG.
  
  
  CONCLUSIONS
  The attenuation by heparin of intestinal ischemia-reperfusion previously demonstrated in rodents could be related with ability of this drug to stimulate and reduce gene expression of encoding anti and pro-apoptotic proteins, respectively.
authors: Department of Surgery, Federal University of Sao Paulo, Brazil.
authors: Murched Omar
authors: MO
authors: Taha
authors: Pharmacology Department, UNIFESP, Sao Paulo, SP, Brazil.
authors: Regiane Miranda
authors: RM
authors: Ferreira
authors: Gynecology Department, UNIFESP, Sao Paulo, SP, Brazil.
authors: Nabiha Saadi Abrahão
authors: NS
authors: Taha
authors: Department of Biochemistry, UNIFESP, Sao Paulo, SP, Brazil.
authors: Hugo Pequeno
authors: HP
authors: Monteiro
authors: Pharmacology Department, UNIFESP, Sao Paulo, SP, Brazil.
authors: Afonso
authors: A
authors: Caricati-Neto
authors: Department of Surgery, UNIFESP, Sao Paulo, SP, Brazil.
authors: Djalma José
authors: DJ
authors: Fagundes
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000187:drug effects* / Q000235:genetics; D003250:Constriction; D015536:Down-Regulation; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D016166:Free Radical Scavengers / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D006493:Heparin / Q000494:pharmacology*; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D007511:Ischemia / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D017538:Mesenteric Artery, Superior; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D051017:Apoptosis Regulatory Proteins; D016166:Free Radical Scavengers; D006493:Heparin