title: The effect of tRNA levels on decoding times of mRNA codons.
abstract: The possible effect of transfer ribonucleic acid (tRNA) concentrations on codons decoding time is a fundamental biomedical research question; however, due to a large number of variables affecting this process and the non-direct relation between them, a conclusive answer to this question has eluded so far researchers in the field. In this study, we perform a novel analysis of the ribosome profiling data of four organisms which enables ranking the decoding times of different codons while filtering translational phenomena such as experimental biases, extreme ribosomal pauses and ribosome traffic jams. Based on this filtering, we show for the first time that there is a significant correlation between tRNA concentrations and the codons estimated decoding time both in prokaryotes and in eukaryotes in natural conditions (-0.38 to -0.66, all P values <0.006); in addition, we show that when considering tRNA concentrations, codons decoding times are not correlated with aminoacyl-tRNA levels. The reported results support the conjecture that translation efficiency is directly influenced by the tRNA levels in the cell. Thus, they should help to understand the evolution of synonymous aspects of coding sequences via the adaptation of their codons to the tRNA pool.
authors: Department of Biomedical Engineering, Sagol School of Neuroscience, Tel-Aviv University, Tel-Aviv 69978, Israel.
authors: Alexandra
authors: A
authors: Dana
authors: Department of Biomedical Engineering, Sagol School of Neuroscience, Tel-Aviv University, Tel-Aviv 69978, Israel tamirtul@post.tau.ac.il.
authors: Tamir
authors: T
authors: Tuller
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D003062:Codon; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism
chemical_list: D003062:Codon; D011506:Proteins; D012333:RNA, Messenger; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases