title: TLR7-expressing cells comprise an interfollicular epidermal stem cell population in murine epidermis.
abstract: Normal interfollicular epidermis (IFE) homeostasis is maintained throughout the entire life by its own stem cells that self-renew and generate progeny that undergo terminal differentiation. However, the fine markers of the stem cells in interfollicular epidermis are not well defined yet. Here we found that TLR7 identified the existence of progenitors and interfollicular epidermal stem cells in murine skin. In vitro, TLR7-expressing cells comprised of two subpopulations that were competent to proliferate and exhibited distinct differentiation potentials. Three-dimensional (3D) organotypic culture and skin reconstitution assays showed that TLR7-expressing cells were able to reconstruct the interfollicular epidermis. Finally, TLR7-expressing cells maintained the intact interfollicular epidermal structures revealed in serial transplantation assays in vivo in mice. Taken together, our results suggest that TLR7-expressing cells comprise an interfollicular epidermal stem cell population.
authors: 1] Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041 [2].
authors: Chaoran
authors: C
authors: Yin
authors: 1] Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041 [2].
authors: Ting
authors: T
authors: Zhang
authors: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041.
authors: Liangjun
authors: L
authors: Qiao
authors: Department of Dermatology, the First Affiliated Hospital, Chongqing Medical University, Chongqing, China 400016.
authors: Jia
authors: J
authors: Du
authors: Department of Dermatology, the First Affiliated Hospital, Chongqing Medical University, Chongqing, China 400016.
authors: Shuang
authors: S
authors: Li
authors: Department of Dermatology, the First Affiliated Hospital, Chongqing Medical University, Chongqing, China 400016.
authors: Hengguang
authors: H
authors: Zhao
authors: Hematology Research Laboratory, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041.
authors: Fangfang
authors: F
authors: Wang
authors: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041.
authors: Qiaorong
authors: Q
authors: Huang
authors: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041.
authors: Wentong
authors: W
authors: Meng
authors: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041.
authors: Hongyan
authors: H
authors: Zhu
authors: Key Laboratory of Transplantation engineering and immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041.
authors: Hong
authors: H
authors: Bu
authors: Department of Dermatology, the First Affiliated Hospital, Chongqing Medical University, Chongqing, China 400016.
authors: Hui
authors: H
authors: Li
authors: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041.
authors: Hong
authors: H
authors: Xu
authors: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041.
authors: Xianming
authors: X
authors: Mo
mesh_terms: D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018859:Hair Follicle / Q000378:metabolism; D000077271:Imiquimod; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000637:transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012038:Regeneration; D012867:Skin / Q000378:metabolism / Q000473:pathology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering; D051199:Toll-Like Receptor 7 / Q000819:agonists / Q000235:genetics / Q000378:metabolism*
chemical_list: D000634:Aminoquinolines; D051199:Toll-Like Receptor 7; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D000077271:Imiquimod