title: Diagnostic investigation of steroid estrogen removal by activated sludge at varying solids retention time.
abstract: The impact of solids retention time (SRT) on estrone (E1), 17β-estradiol (E2), estriol (E3) and 17α-ethinylestradiol (EE2) removal in an activated sludge plant (ASP) was examined using a pilot plant to closely control operation. Exsitu analytical methods were simultaneously used to enable discrimination of the dominant mechanisms governing estrogen removal following transitions in SRT from short (3d) to medium (10d) and long (27d) SRTs which broadly represent those encountered at full-scale. Total estrogen (∑EST, i.e., sum of E1, E2, E3 and EE2) removals which account for aqueous and particulate concentrations were 70±8, 95±1 and 93±2% at 3, 10 and 27d SRTs respectively. The improved removal observed following an SRT increase from 3 to 10d was attributable to the augmented biodegradation of the natural estrogens E1 and E2. Interestingly, estrogen biodegradation per bacterial cell increased with SRT. These were 499, 1361 and 1750ng 10(12) viable cells(-1)d(-1). This indicated an improved efficiency of the same group or the development of a more responsive group of bacteria. In this study no improvement in absolute ∑EST removal was observed in the ASP when SRT increased from 10 to 27d. However, batch studies identified an augmented biomass sorption capacity for the more hydrophobic estrogens E2 and EE2 at 27d, equivalent to an order of magnitude. The lack of influence on estrogen removal during pilot plant operation can be ascribed to their distribution within activated sludge being under equilibrium. Consequently, lower wastage of excess sludge inherent of long SRT operation counteracts any improvement in sorption.
authors: Cranfield Water Science Institute, Cranfield University, Bedfordshire MK43 0AL, UK.
authors: Bruce
authors: B
authors: Petrie
authors: Cranfield Water Science Institute, Cranfield University, Bedfordshire MK43 0AL, UK.
authors: Ewan J
authors: EJ
authors: McAdam
authors: Cranfield Water Science Institute, Cranfield University, Bedfordshire MK43 0AL, UK.
authors: Francis
authors: F
authors: Hassard
authors: Cranfield Water Science Institute, Cranfield University, Bedfordshire MK43 0AL, UK.
authors: Tom
authors: T
authors: Stephenson
authors: Cranfield Water Science Institute, Cranfield University, Bedfordshire MK43 0AL, UK.
authors: John N
authors: JN
authors: Lester
authors: Cranfield Water Science Institute, Cranfield University, Bedfordshire MK43 0AL, UK. Electronic address: e.cartmell@cranfield.ac.uk.
authors: Elise
authors: E
authors: Cartmell
mesh_terms: D001673:Biodegradation, Environmental; D004739:England; D004958:Estradiol / Q000032:analysis / Q000378:metabolism; D045166:Estradiol Congeners / Q000032:analysis / Q000378:metabolism*; D004964:Estriol / Q000032:analysis / Q000378:metabolism; D004970:Estrone / Q000032:analysis / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000032:analysis / Q000378:metabolism; D010865:Pilot Projects; D012722:Sewage / Q000382:microbiology*; D013997:Time Factors; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D045166:Estradiol Congeners; D012722:Sewage; D014874:Water Pollutants, Chemical; D004970:Estrone; D004997:Ethinyl Estradiol; D004958:Estradiol; D004964:Estriol
keywords: Diffusion; Enzyme; Extracellular; Physicochemical; Sewage