title: A nineteen gene-based risk score classifier predicts prognosis of colorectal cancer patients.
abstract: Colorectal cancer (CRC) patients frequently experience disease recurrence and distant metastasis. This study aimed to identify prognostic indicators, including individual responses to chemotherapy, in CRC patients. RNA-seq data was generated using 54 samples (normal colon, primary CRC, and liver metastases) from 18 CRC patients and genes associated with CRC aggressiveness were identified. A risk score based on these genes was developed and validated in four independent CRC patient cohorts (n = 1063). Diverse statistical methods were applied to validate the risk scoring system, including a generalized linear model likelihood ratio test, Kaplan-Meier curves, a log-rank test, and the Cox model. TREM1 and CTGF were identified as two activated regulators associated with CRC aggressiveness. A risk score based on 19 genes regulated by TREM1 or CTGF activation (TCA19) was a significant prognostic indicator. In multivariate and subset analyses based on pathological staging, TCA19 was an independent risk factor (HR = 1.894, 95% CI = 1.227-2.809, P = 0.002). Subset stratification in stage III patients revealed that TCA19 had prognostic potential and identified patients who would benefit from adjuvant chemotherapy, regardless of age. The TCA19 predictor represents a novel diagnostic tool for identifying high-risk CRC patients and possibly predicting the response to adjuvant chemotherapy.
authors: Medical Genomics Research Centre, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
authors: Seon-Kyu
authors: SK
authors: Kim
authors: Medical Genomics Research Centre, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
authors: Seon-Young
authors: SY
authors: Kim
authors: Medical Genomics Research Centre, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
authors: Jeong-Hwan
authors: JH
authors: Kim
authors: Department of Surgery, University of Ulsan College of Medicine, Seoul, Korea; Department of Cancer Research, Institute of Innovative Cancer Research and Asan Institute for Life Sciences, Asan Medical Centre, Seoul, Korea.
authors: Seon Ae
authors: SA
authors: Roh
authors: Department of Cancer Research, Institute of Innovative Cancer Research and Asan Institute for Life Sciences, Asan Medical Centre, Seoul, Korea; Graduate School of East-West Medical Science, Kyung Hee University, Gyeonggi-do, Korea.
authors: Dong-Hyung
authors: DH
authors: Cho
authors: Medical Genomics Research Centre, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea; Department of Cancer Research, Institute of Innovative Cancer Research and Asan Institute for Life Sciences, Asan Medical Centre, Seoul, Korea. Electronic address: yongsung@kribb.re.kr.
authors: Yong Sung
authors: YS
authors: Kim
authors: Department of Surgery, University of Ulsan College of Medicine, Seoul, Korea; Department of Cancer Research, Institute of Innovative Cancer Research and Asan Institute for Life Sciences, Asan Medical Centre, Seoul, Korea. Electronic address: jckim@amc.seoul.kr.
authors: Jin Cheon
authors: JC
authors: Kim
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology*; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
chemical_list: C526317:CCN2 protein, human; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D055513:Connective Tissue Growth Factor
keywords: Chemotherapy; Colorectal cancer; Markers; Metastasis; Prognosis