title: Astrocyte-like cells differentiated from a novel population of CD45-positive cells in adult human peripheral blood.
abstract: We have previously reported a novel CD45-positive cell population called peripheral blood insulin-producing cells (PB-IPCs) and its unique potential for releasing insulin in vitro. Despite the CD45-positive phenotype and self-renewal ability, PB-IPCs are distinguished from hemopoietic and endothelial progenitor cells (EPCs) by some characteristics, such as a CD34-negative phenotype and different culture conditions. We have further identified the gene profiles of the embryonic and neural stem cells, and these profiles include Sox2, Nanog, c-Myc, Klf4, Notch1 and Mash1. After treatment with all-trans retinoic acid (ATRA) in vitro, most PB-IPCs exhibited morphological changes that included the development of elongated and branched cell processes. In the process of induction, the mRNA expression of Hes1 was robustly upregulated, and a majority of cells acquired some astrocyte-associated specific phenotypes including anti-glial fibrillary acidic protein (GFAP), CD44, Glutamate-aspartate transporter (GLAST) and S100β. In spite of the deficiency of glutamate uptaking, the differentiated cells significantly relaxed the regulation of the expression of brain-derived neurotrophic factor (BDNF) mRNA. This finding demonstrates that PB-IPCs could be induced into a population of astrocyte-like cells and enhanced the neurotrophic potential when the state of proliferation was limited by ATRA, which implies that this unique CD45+ cell pool may have a protective role in some degenerative diseases of the central nervous system (CNS).
authors: Department of Neurology, Jinan Central Hospital Affiliated to Shandong University, China.
authors: Heng
authors: H
authors: Li
authors: Jun
authors: J
authors: Li
authors: Wenhua
authors: W
authors: Sheng
authors: Jinhao
authors: J
authors: Sun
authors: Xiaoli
authors: X
authors: Ma
authors: Xueran
authors: X
authors: Chen
authors: Jianfen
authors: J
authors: Bi
authors: Yong
authors: Y
authors: Zhao
authors: Xiaohong
authors: X
authors: Li
mesh_terms: D000328:Adult; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors; D018698:Glutamic Acid; D014212:Tretinoin; D017493:Leukocyte Common Antigens
keywords: all-trans retinoic acid; astrocytes; differentiation; human peripheral blood; insulin-producing cells