title: Ameliorating effects of Juzentaihoto on restraint stress and P. gingivalis-induced alveolar bone loss.
abstract: OBJECTIVE
  Juzentaihoto (JTX) is a traditional Japanese medicine that consists of 10 herbs. The purpose of this study was to evaluate the efficacy of multi-herbal medicine JTX as a preventive and therapeutic drug for periodontal bone resorption and for reducing restraint stress.
  
  
  METHODS
  Porphyromonas gingivalis ATCC 33277 was used for testing the antibacterial activity of JTX and a rat experimental periodontitis model. To evaluate the effect of JTX against P. gingivalis infection, we determined the differences in alveolar bone loss among experimental groups. The concentrations of adrenocorticotropic hormones were measured as stress markers, and atrophy of the thymus and spleen was assessed.
  
  
  RESULTS
  JTX had antibacterial activity against P. gingivalis ATCC 33277. JTX treatment of mouse bone marrow cells at a concentration of 0.1 μg/ml significantly inhibited osteoclast formation. Administration of JTX to rats with P. gingivalis infection and restraint stress significantly reduced alveolar bone loss compared with the case with just the combination of P. gingivalis infection and restraint stress. In the restrained groups, stress markers were elevated, and the thymus and spleen were atrophied. The groups with administration of JTX showed not only inhibition of the decrease of weight but also normalization of corticosterone and cortisol values.
  
  
  CONCLUSIONS
  JTX effectively inhibited restraint stress and osteoclastogenesis. It appears that the effects of JTX inhibit the destruction of periodontal tissue by suppressing stress. Our study demonstrated that JTX affects the correlation between restraint stress and periodontitis.
authors: Department of Orthodontics Science, Kanagawa Dental University, Yokosuka, Japan.
authors: Orie
authors: O
authors: Takeda
authors: Department of Microbiology, Kanagawa Dental University, Yokosuka, Japan.
authors: Toshizo
authors: T
authors: Toyama
authors: Department of Microbiology, Kanagawa Dental University, Yokosuka, Japan.
authors: Kiyoko
authors: K
authors: Watanabe
authors: Department of Microbiology, Kanagawa Dental University, Yokosuka, Japan.
authors: Takenori
authors: T
authors: Sato
authors: Department of Orthodontics Science, Kanagawa Dental University, Yokosuka, Japan.
authors: Kenichi
authors: K
authors: Sasaguri
authors: Department of Orthodontics Science, Kanagawa Dental University, Yokosuka, Japan.
authors: Susumu
authors: S
authors: Akimoto
authors: Department of Orthodontics Science, Kanagawa Dental University, Yokosuka, Japan.
authors: Sadao
authors: S
authors: Sato
authors: Department of Orthodontics Science, Kanagawa Dental University, Yokosuka, Japan.
authors: Toshitsugu
authors: T
authors: Kawata
authors: Department of Microbiology, Kanagawa Dental University, Yokosuka, Japan. Electronic address: hamada@kdu.ac.jp.
authors: Nobushiro
authors: N
authors: Hamada
mesh_terms: D016301:Alveolar Bone Loss / Q000382:microbiology / Q000517:prevention & control*; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D002470:Cell Survival; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010010:Osteoclasts / Q000187:drug effects; D010518:Periodontitis / Q000188:drug therapy / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; C063587:juzentaihoto
keywords: Alveolar bone loss; Juzentaihoto; Porphyromonas gingivalis; Stress