title: Isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of Medicago truncatula plants.
abstract: In the present study, we analyzed the frequency of hemolytic and antifungal activities in bacterial isolates from the rhizosphere of Medicago truncatula plants. Of the 2000 bacterial colonies, 96 showed β-hemolytic activities (frequency, 4.8 x 10(-2)). Hemolytic isolates were analyzed for their genetic diversity by using random amplification of polymorphic DNA, yielding 88 haplotypes. The similarity coefficient of Nei and Li showed a polymorphic diversity ranging from 0.3 to 1. Additionally, 8 of the hemolytic isolates showed antifungal activity toward plant pathogens, Diaporthe phaseolorum, Colletotrichum acutatum, Rhizoctonia solani, and Fusarium oxysporum. The 16S ribosomal sequencing analysis showed that antagonistic bacterial isolates corresponded to Bacillus subtilis (UM15, UM33, UM42, UM49, UM52, and UM91), Bacillus pumilus (UM24), and Bacillus licheniformis (UM88). The present results revealed a higher genetic diversity among hemolytic isolates compared to that of isolates with antifungal action.
authors: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México.
authors: J E
authors: JE
authors: Hernández-Salmerón
authors: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México.
authors: C M
authors: CM
authors: Prieto-Barajas
authors: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México.
authors: E
authors: E
authors: Valencia-Cantero
authors: Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada.
authors: G
authors: G
authors: Moreno-Hagelsieb
authors: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México gustavo_santoyo@yahoo.com.
authors: G
authors: G
authors: Santoyo
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D001407:Bacillus / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001412:Bacillus subtilis / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development; D014644:Genetic Variation; D006239:Haplotypes; D006461:Hemolysis; D046913:Medicago truncatula / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D012232:Rhizoctonia / Q000187:drug effects / Q000254:growth & development; D058441:Rhizosphere; D012988:Soil Microbiology
chemical_list: D000935:Antifungal Agents; D012336:RNA, Ribosomal, 16S