title: Multiplex immunoassays for quantification of cytokines, growth factors, and other proteins in stem cell communication.
abstract: Immunoassays represent valuable and broadly used techniques for detection and quantification of proteins. Thanks to their high sensitivity, such techniques are powerful for analyzing growth factors, trophic factors, angiogenic factors, hormones, cytokines, chemokines, soluble receptors, and other proteins which play key roles in intercellular communication and operate as potent regulators of stem cell survival, proliferation, differentiation, or cell death. Multiplex immunological assays, in contrast to ELISA, offer simultaneous quantification of tens of proteins across multiple samples, and have been developed to save time, costs, and sample volumes. Among them, planar antibody microarrays and xMAP(®) bead-based assays have become particularly popular for characterization of proteins secreted by stem cells, as they are relatively easy, highly accurate, multiplex to a high degree and a broad spectrum of analytes can be measured. Here, we describe protocols for multiplex quantification of secreted proteins using Quantibody(®) microarrays (RayBiotech) and xMAP(®) assays (Luminex and its partners).
authors: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburska 89, 27721, Libechov, Czech Republic.
authors: Ivona
authors: I
authors: Valekova
authors: Helena Kupcova
authors: HK
authors: Skalnikova
authors: Karla
authors: K
authors: Jarkovska
authors: Jan
authors: J
authors: Motlik
authors: Hana
authors: H
authors: Kovarova
mesh_terms: D002450:Cell Communication; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D007118:Immunoassay / Q000379:methods*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D013234:Stem Cells / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins