title: SIVQ-LCM protocol for the ArcturusXT instrument.
abstract: SIVQ-LCM is a new methodology that automates and streamlines the more traditional, user-dependent laser dissection process. It aims to create an advanced, rapidly customizable laser dissection platform technology. In this report, we describe the integration of the image analysis software Spatially Invariant Vector Quantization (SIVQ) onto the ArcturusXT instrument. The ArcturusXT system contains both an infrared (IR) and ultraviolet (UV) laser, allowing for specific cell or large area dissections. The principal goal is to improve the speed, accuracy, and reproducibility of the laser dissection to increase sample throughput. This novel approach facilitates microdissection of both animal and human tissues in research and clinical workflows.
authors: Laboratory of Pathology, National Cancer Institute, National Institutes of Health.
authors: Jason D
authors: JD
authors: Hipp
authors: Department of Pathology, University of Michigan.
authors: Jerome
authors: J
authors: Cheng
authors: Laboratory of Pathology, National Cancer Institute, National Institutes of Health.
authors: Jeffrey C
authors: JC
authors: Hanson
authors: Laboratory of Pathology, National Cancer Institute, National Institutes of Health.
authors: Avi Z
authors: AZ
authors: Rosenberg
authors: Laboratory of Pathology, National Cancer Institute, National Institutes of Health.
authors: Michael R
authors: MR
authors: Emmert-Buck
authors: Laboratory of Pathology, National Cancer Institute, National Institutes of Health; tangream@mail.nih.gov.
authors: Michael A
authors: MA
authors: Tangrea
authors: Department of Pathology, University of Michigan.
authors: Ulysses J
authors: UJ
authors: Balis
mesh_terms: D000465:Algorithms; D000818:Animals; D001331:Automation / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D061346:Laser Capture Microdissection / Q000295:instrumentation / Q000379:methods*; D010363:Pattern Recognition, Automated