title: Development of an indirect immunofluorescence technique for the evaluation of generated antibody titers against Erysipelothrix rhusiopathiae in captive bottlenose dolphins (Tursiops truncatus).
abstract: Erysipelothrix rhusiopathiae is the causative agent of erysipelas, a disease of many mammalian and avian species, mainly swine and turkeys. In cetaceans, erysipelas is considered to be the most common infection in juvenile individuals, which have not been vaccinated. Moreover, the disease manifest in both forms, the dermatologic and the acute septicemic forms, has been reported in various species of dolphins and whales. It is difficult to diagnose erysipelas by currently available approaches. Moreover, it is mainly based on culture methods and also PCR methods, which are currently being developed. At the present stage, prophylactic approaches are based on antibiotic therapy and vaccination mostly with porcine erysipelas vaccines. In the present study, an Indirect Immuno Fluorescence method for the detection of dolphin antibodies levels against E. rhusiopathiae was developed and applied in two different groups of captive bottlenose dolphins (Tursiops truncatus) from Loro Parque (Tenerife, Canary Islands, Spain) and L'Oceanogràfic de Valencia (Valencia, Spain) in order to check the tittering levels of antibodies after application of porcine erysipelas vaccines in the studied dolphins.
authors: Loro Parque, Avda. Loro Parque SN, 38400, Puerto de la Cruz, Tenerife, Spain, mjosebernalg@yahoo.es.
authors: María José
authors: MJ
authors: Bernal-Guadarrama
authors: Daniel
authors: D
authors: García-Parraga
authors: Nuhacet
authors: N
authors: Fernández-Gallardo
authors: Rafael
authors: R
authors: Zamora-Padrón
authors: Víctor
authors: V
authors: Pacheco
authors: María
authors: M
authors: Reyes-Batlle
authors: Basilio
authors: B
authors: Valladares
authors: Jacob
authors: J
authors: Lorenzo-Morales
authors: Enrique
authors: E
authors: Martínez-Carretero
mesh_terms: D000818:Animals; D000836:Animals, Zoo; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines / Q000276:immunology; D050681:Bottle-Nosed Dolphin; D004888:Erysipelothrix / Q000276:immunology*; D004889:Erysipelothrix Infections / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary*; D008297:Male; D013030:Spain; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines