title: Comparison of the effects of CORM-2, CORM-3 and CORM-A1 on coagulation in human plasma.
abstract: Carbon monoxide derived from the catalytic action of heme oxygenase-1 or carbon monoxide-releasing molecules (CORMs) has been found to potentially be an anticoagulant or procoagulant agent. Of interest, two water-soluble CORMs, CORM-3 and CORM-A1, recently became commercially available. Thus, the purpose of the present study was to assess and compare the effects of the previously well studied CORM-2 to the effects of CORM-3 and CORM-A1 on coagulation in citrated human plasma with thrombelastography. Plasma exposed to CORMs was incubated at 37°C for at least one carbon monoxide release half-time, and then tissue factor-activated coagulation was commenced with calcium addition. CORM-2 and CORM-3 enhanced the velocity of clot formation and thrombus strength in a similar manner, whereas CORM-A1 did not affect coagulation. However, CORM-A1 did diminish tissue-type plasminogen activator initiated fibrinolysis. The similarity in effect on coagulation by CORM-2 and CORM-3 was likely secondary to the relatively inert effect of their ruthenium-containing carrier molecule, whereas the boron-containing CORM-A1 may have had no effect secondary to boron binding to fibrinogen, preventing carbon monoxide-mediated changes in fibrinogen protein structure via attached heme group(s). Future investigations with CORMs should have special attention to confounding effects of the carrier molecule.
authors: Department of Anesthesiology, The University of Arizona College of Medicine, Tucson, Arizona, USA.
authors: Vance G
authors: VG
authors: Nielsen
authors: Joshua I
authors: JI
authors: Garza
mesh_terms: D001777:Blood Coagulation; D001880:Boranes / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002248:Carbon Monoxide / Q000737:chemistry; D002254:Carbonates / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D009942:Organometallic Compounds / Q000737:chemistry*; D010949:Plasma / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013916:Thrombelastography; D013925:Thromboplastin / Q000737:chemistry; D010959:Tissue Plasminogen Activator / Q000737:chemistry
chemical_list: D001880:Boranes; D002254:Carbonates; D009942:Organometallic Compounds; C503854:sodium boranocarbonate; C476530:tricarbonylchloro(glycinato)ruthenium(II); C447082:tricarbonyldichlororuthenium (II) dimer; D002248:Carbon Monoxide; D005340:Fibrinogen; D013925:Thromboplastin; D010959:Tissue Plasminogen Activator; D002118:Calcium