title: Reduced fibroblast adhesion and proliferation on plasma-modified titanium surfaces.
abstract: Soft tissue complications are clinically relevant problems after osteosynthesis of fractures. The goal is to develop a method for reduction of fibroblast adhesion and proliferation on titanium implant surfaces by plasma polymerisation of the organo-silicon monomer hexamethyldisiloxane (HMDSO). HMDSO was deposited under continuous wave conditions in excess oxygen (ppHMDSO surface) and selected samples were further modified with an additional oxygen plasma (ppHMDSO + O2 surface). Surface characterization was performed by scanning electron microscopy, profilometry, water contact angle measurements, infrared reflection absorption spectroscopy and X-ray photoelectron spectroscopy. In our experimental setup the mechanical properties, roughness and topography of the titanium were preserved, while surface chemistry was drastically changed. Fibroblast proliferation was assessed by alamarBlue assay, cell morphology by confocal microscopy visualization of eGFP-transducted fibroblasts, and cell viability by Annexine V/propidium iodide assay. Both modified surfaces, non-activated hydrophobic ppHMDSO and activated hydrophilic ppHMDSO + O2 were able to dramatically reduce fibroblast colonization and proliferation compared to standard titanium. However, this effect was more strongly pronounced on the hydrophobic ppHMDSO surface, which caused reduced cell adhesion and prevented proliferation of fibroblasts. The results demonstrate that plasma modifications of titanium using HMDSO are valuable candidates for future developments in anti-adhesive and anti-proliferative coatings for titanium fracture implants.
authors: Department of Orthopedics and Traumatology, BiomaTiCS Research Group, University Medical Centre of the Johannes Gutenberg University, Langenbeckstr. 1, 55101, Mainz, Germany, sebastian.kuhn@unimedizin-mainz.de.
authors: Sebastian
authors: S
authors: Kuhn
authors: Jennifer
authors: J
authors: Kroth
authors: Ulrike
authors: U
authors: Ritz
authors: Alexander
authors: A
authors: Hofmann
authors: Christian
authors: C
authors: Brendel
authors: Lars Peter
authors: LP
authors: Müller
authors: Renate
authors: R
authors: Förch
authors: Pol Maria
authors: PM
authors: Rommens
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D006801:Humans; D058626:Plasma Gases / Q000737:chemistry*; D012833:Siloxanes / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D058626:Plasma Gases; D012833:Siloxanes; D014025:Titanium; C015917:hexamethyldisiloxane