title: [Lentiviral-mediated RNA interference of LXRα gene in donor rats with fatty liver enhances liver graft function after transplantation].
abstract: OBJECTIVE
  To investigate whether RNA interference (RNAi) of LXRα gene in donor rats with fatty liver improves liver graft function after transplantation.
  
  
  METHODS
  Fifty donor SD rats were fed a high-fat diet and 56% alcohol to induce macrovesicular steatosis exceeding 60% in the liver. The donor rats were injected via the portal veins with 7 × 10⁷ TU LXRα-RNAi-LV mixture (n=25) or negative control-LV (NC-LV) vector (n=25) 72 h before orthotopic liver transplantation. At 2, 24, and 72 h after the transplantation, the recipient rats were sacrificed to examine liver transaminases, liver graft histology, immunostaining (TUNEL), and protein and mRNA levels of LXRα.
  
  
  RESULTS
  Lentivirus-LXRα RNAi inhibited LXRα gene expression at both the mRNA and protein levels in the liver graft and reduced the expressions of SREBP-1c and CD36 as compared with the controls, resulting also in reduced fatty acid accumulation in the hepatocytes. The recipient rats receiving RNAi-treated grafts showed more obvious reduction in serum ALT, AST, IL-1β and TNF-α levels, and exhibited milder hepatic pathologies than the control rats after the transplantation. TUNEL assay demonstrated a significant reduction in cell apoptosis in LXRα-RNAi-LV-treated liver grafts, and the rats receiving treated liver grafts had a prolonged mean overall survival time.
  
  
  CONCLUSIONS
  LXRα-RNAi-LV treatment of the donor rats with fatty liver can significantly down-regulate LXRα gene expression in the liver graft and improve the graft function and recipient rat survival after liver transplantation.
authors: Department of Hepatobiliary and Transplantation Surgery, Ganmei Affiliated Hospital, Kunming Medical University, Kunming 650011, China. E-mail: prisoner33230393@hotmail.com.
authors: Yingpeng
authors: Y
authors: Zhao
authors: Li
authors: L
authors: Li
authors: Jingpan
authors: J
authors: Ma
authors: Gang
authors: G
authors: Chen
authors: Jianhua
authors: J
authors: Bai
mesh_terms: D000818:Animals; D005234:Fatty Liver / Q000235:genetics / Q000601:surgery*; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000166:cytology; D016086:Lentivirus; D008099:Liver / Q000502:physiology*; D016031:Liver Transplantation; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000071518:Liver X Receptors; C000605215:Nr1h3 protein, rat; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger