title: Identification of phenolic antioxidants in Ipomoea mauritiana jacq. using spectrophotometric and mass spectroscopic studies.
abstract: OBJECTIVE
  Ipomoea mauritiana is used in both Ayurveda and folk medicine systems. The tuberous roots are known to be diuretic, depurative, carminative, and anthelmintic. The objective of the current study was to identify phenolic antioxidants from I . mauritiana using spectrophotometric and LC-MS analysis.
  
  
  METHODS
  An activity-guided fractionation and puriﬁcation process was used to identify the antioxidative components from I . mauritiana tuber. Dried mature tubers of I. mauritiana were extracted with 80% methanol and then partitioned by chloroform, ethyl acetate, acetone, and methanol. The acetone fraction showed the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity among four fractions and was subjected to separation and puriﬁcation using preparative reverse-phase high-performance liquid chromatography (HPLC).
  
  
  RESULTS
  Two compounds were separated from the acetone fraction using preparative LC fraction collector. The puriﬁed compounds were screened for their antioxidative potential using DPPH assay. The compounds were subjected to LC-MS analysis in ESI negative mode. One of the compounds was identified as Caffeoyl glucose based on the mass fragmentation.
  
  
  CONCLUSIONS
  The acetone fraction showed highest radical scavenging activity and the phytoconstituents of the same were identified by LC-MS/MS analysis.
authors: Centre for Medicinal Plants Research, Arya Vaidya Sala Kottakkal-676503, Kerala, India.
authors: Cheruthazhakkat
authors: C
authors: Sulaiman
authors: Centre for Medicinal Plants Research, Arya Vaidya Sala Kottakkal-676503, Kerala, India.
authors: Sivadasan Pillai
authors: SP
authors: Geetha
authors: Centre for Medicinal Plants Research, Arya Vaidya Sala Kottakkal-676503, Kerala, India.
authors: Balachandran
authors: B
authors: Indira
keywords: DPPH; Ipomoea mauritiana; LC-ESI-MS; Phenolics