title: Expression of sulfur uptake assimilation-related genes in response to cadmium, bensulfuron-methyl and their co-contamination in rice roots.
abstract: The responses of sulfur (S) uptake assimilation-related genes' expression in roots of two rice cultivars to cadmium (Cd), bensulfuron-methyl (BSM) and their co-contamination (Cd+BSM) were investigated by gene-chip microarray analysis and quantitative real-time PCR (QRT-PCR) technology. Treatments of Cd and Cd+BSM induced expression of sulfate transporter and permease genes, and promoted sulfate uptake in rice roots. Cd+BSM could alleviate Cd toxicity to cv. Fengmeizhan seedlings, probably due to Cd+BSM promoting greater S absorption by seedlings. Cd and Cd+BSM induced expression of sulfate assimilation-related genes, and thus activated the sulfur assimilation pathway. Cd and Cd+BSM induced expression of phytochelatin synthase and metallothionein genes, and induced expression of glutathione S-transferases (GSTs), glutathione synthase (GS) and S-containing antioxidation enzyme genes, which detoxified Cd(2+). It is suggested that (to cope with the toxicity of Cd, BSM and their co-contamination) the S uptake and assimilation pathway was activated in rice roots by increased expression of related genes, thus enhancing the supply of organic S for synthesis of Cd or BSM resistance-related substances.
authors: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
authors: Jian
authors: J
authors: Zhou
authors: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China. Electronic address: wz0162@126.com.
authors: Zegang
authors: Z
authors: Wang
authors: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
authors: Zhiwei
authors: Z
authors: Huang
authors: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors: Chao
authors: C
authors: Lu
authors: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
authors: Zhuo
authors: Z
authors: Han
authors: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors: Jianfeng
authors: J
authors: Zhang
authors: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors: Huimin
authors: H
authors: Jiang
authors: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China. Electronic address: gecailin10@163.com.
authors: Cailin
authors: C
authors: Ge
authors: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: yangjch@263.net.
authors: Juncheng
authors: J
authors: Yang
mesh_terms: D002104:Cadmium / Q000633:toxicity*; D020869:Gene Expression Profiling; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D013453:Sulfonylurea Compounds / Q000633:toxicity*; D013455:Sulfur / Q000378:metabolism*
chemical_list: D013453:Sulfonylurea Compounds; D002104:Cadmium; D013455:Sulfur; C071475:methyl bensulfuron
keywords: bensulfuron-methyl; cadmium; rice; sulfur assimilation