title: Transcriptomic response to Yersinia pestis: RIG-I like receptor signaling response is detrimental to the host against plague.
abstract: Bacterial pathogens have evolved various mechanisms to modulate host immune responses for successful infection. In this study, RNA-sequencing technology was used to analyze the responses of human monocytes THP1 to Yersinia pestis infection. Over 6000 genes were differentially expressed over the 12 h infection. Kinetic responses of pathogen recognition receptor signaling pathways, apoptosis, antigen processing, and presentation pathway and coagulation system were analyzed in detail. Among them, RIG-I-like receptor (RLR) signaling pathway, which was established for antiviral defense, was significantly affected. Mice lacking MAVS, the adaptor of the RLR signaling pathway, were less sensitive to infection and exhibited lower IFN-β production, higher Th1-type cytokines IFN-γ and IL-12 production, and lower Th2-type cytokines IL-4 and IL-13 production in the serum compared with wild-type mice. Moreover, infection of pathogenic bacteria other than Y. pestis also altered the expression of the RLR pathway, suggesting that the response of RLR pathway to bacterial infection is a universal mechanism.
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China. Electronic address: zongmindu@gmail.com.
authors: Zongmin
authors: Z
authors: Du
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Huiying
authors: H
authors: Yang
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Yafang
authors: Y
authors: Tan
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Guang
authors: G
authors: Tian
authors: Qinghai Institute for Endemic Disease Prevention and Control of Qinghai Province, Xining 811602, China.
authors: Qingwen
authors: Q
authors: Zhang
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Yujun
authors: Y
authors: Cui
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Yanfeng Yan
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Xiaohong
authors: X
authors: Wu
authors: BGI-Shenzhen, Shenzhen 518083, China.
authors: Zuyun
authors: Z
authors: Chen
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Shiyang
authors: S
authors: Cao
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Yujing
authors: Y
authors: Bi
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Yanping
authors: Y
authors: Han
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Xiaoyi
authors: X
authors: Wang
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors: Yajun
authors: Y
authors: Song
authors: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China. Electronic address: ruifuyang@gmail.com.
authors: Ruifu
authors: R
authors: Yang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000172:deficiency; D000818:Animals; D017951:Antigen Presentation; D000942:Antigens, Bacterial / Q000276:immunology; D017209:Apoptosis; D001777:Blood Coagulation; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D051379:Mice; D008810:Mice, Inbred C57BL; D010930:Plague / Q000235:genetics* / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D011971:Receptors, Immunologic; D015398:Signal Transduction; D015010:Yersinia pestis / Q000276:immunology / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000942:Antigens, Bacterial; D016207:Cytokines; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D011971:Receptors, Immunologic; C512612:MAVS protein, mouse; C505371:MAVS protein, human; C452820:RIGI protein, human
keywords: Innate immunity; RIG-I-like receptor signaling; RNA-seq; Transcriptomic response; Yersinia pestis