title: Chitosan as an adjuvant-like substrate for dendritic cell culture to enhance antitumor effects.
abstract: To induce monocyte differentiation into dendritic cells (DCs) is the essential protocol for the DC-mediated cancer immunotherapy. In this study, monocytes isolated from mouse bone marrow were cultured on chitosan substrate to evaluate the effect of the chitosan culture system on the induction and tumor protection of DCs. Compared to tissue culture polystyrene (TCPS), the chitosan culture system could enhance monocyte aggregation and detachment with increased MTT reduction activity and expression of DC marker CD11c and LPS co-receptor CD14. Moreover, compared to TCPS, chitosan could enhance lipopolysaccharides (LPS)-stimulated DCs to secrete higher amount of IL-12. More importantly, vaccination of tumor lysate-pulsed DCs harvested from chitosan could increase cytotoxic T-lymphocyte (CTL) activity and showed significantly enhanced anti-tumor effect than those from TCPS. Therefore, the current study demonstrated that a protocol to culture DCs on a less-adherent chitosan substrate followed by treatment with tumor lysate has the potential in future DC-based vaccine application.
authors: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.
authors: Yong-Chong
authors: YC
authors: Lin
authors: Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan. Electronic address: pjlou@ntu.edu.tw.
authors: Pei-Jen
authors: PJ
authors: Lou
authors: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan. Electronic address: thyoung@ntu.edu.tw.
authors: Tai-Horng
authors: TH
authors: Young
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D019496:Cancer Vaccines / Q000276:immunology / Q000627:therapeutic use*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D009369:Neoplasms / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000276:Adjuvants, Immunologic; D001672:Biocompatible Materials; D019496:Cancer Vaccines; D048271:Chitosan
keywords: Chitosan; Dendritic cell; Tumor; Vaccine