title: Cyclic RGD-modified chitosan/graphene oxide polymers for drug delivery and cellular imaging.
abstract: Polymers based on cyclic RGD-modified chitosan/graphene oxide are investigated in this paper as an innovative type of drug delivery system for hepatocellular carcinoma-targeted therapy and imaging. The system was prepared using a simple noncovalent method by coating drug-loaded graphene oxide (GO) with cyclic RGD-modified chitosan (RC). The results show that an efficient loading of doxorubicin (DOX) on GO (1.00mg/mg) was obtained. The system exhibits a pH-responsive behavior because of the hydrogen bonding interaction between GO and RC, and may be very stable under physiological conditions but with release at a lower pH (tumor environment). In addition, cellular uptake and proliferation studies using hepatoma cells (Bel-7402, SMMC-7721, HepG2) indicated that the cRGD-modified chitosan/graphene oxide polymer could recognize hepatoma cells and promote drug uptake by the cells, especially for cells overexpressing integrins. Together, these results demonstrate that the RC/GO polymers provide a multifunctional drug delivery system with the ability to target hepatocarcinoma cells, and are pH-responsive and can be efficiently loaded with a number of therapeutic agents for biomedical applications.
authors: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, PR China; School of Pharmacy, Xiamen Medical College, Xiamen 361008, PR China.
authors: Chen
authors: C
authors: Wang
authors: Department of Pharmacy, Xiamen Xianyue Hospital, 361012, PR China.
authors: Binbin
authors: B
authors: Chen
authors: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
authors: Meijuan
authors: M
authors: Zou
authors: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, PR China. Electronic address: chenggang63@hotmail.com.
authors: Gang
authors: G
authors: Cheng
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage; D004337:Drug Carriers; D006108:Graphite / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D010456:Peptides, Cyclic / Q000008:administration & dosage / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D010456:Peptides, Cyclic; C507169:cyclic arginine-glycine-aspartic acid peptide; D006108:Graphite; D004317:Doxorubicin; D048271:Chitosan
keywords: Controlled release; Cyclic RGD; Graphene oxide; Hepatoma cells targeting; pH-responsive