title: Fumigant toxicity and acetylcholinesterase inhibitory activity of 4 Asteraceae plant essential oils and their constituents against Japanese termite (Reticulitermes speratus Kolbe).
abstract: This study investigated the fumigant toxicity of 4 Asteraceae plant essential oils and their constituents against the Japanese termite Reticulitermes speratus Kolbe. Fumigant toxicity varied with plant essential oils or constituents, exposure time, and concentration. Among the tested essential oils, those from Chamaemelum nobile exhibited the strongest fumigant toxicity, followed by those from Santolina chamaecyparissus, Ormenis multicaulis, and Eriocephalus punctulatus at 2 days after treatment. In all, 15, 24, 19, and 9 compounds were identified in the essential oils from C. nobile, E. punctulatus, O. multicaulis, and S. chamaecyparissus, respectively, by using gas chromatography, gas chromatography-mass spectrometry, or open-column chromatography. The identified compounds were tested individually for their fumigant toxicity against Japanese termites. Among the test compounds, trans-pinocarveol, caryophyllene oxide, sabinene hydrate, and santolina alcohol showed strong fumigant toxicity against Japanese termites. Acetylcholinesterase (AChE) inhibition activity of the identified compounds from C. nobile, E. punctulatus, O. multicaulis, and S. chamaecyparissus essential oils were tested to determine the mode of their action. The IC50 values of (+)-α-pinene, (-)-limonene, (-)-α-pinene, β-pinene, and β-phellandrene against Japanese termite AChE were 0.03, 0.13, 0.41, 0.42, and 0.67mg/mL, respectively. Further studies are warranted to determine the potential of these essential oils and their constituents as fumigants for termite control.
authors: Lifetree Biotech Co., Ltd., Maesonggosaek-ro, Kwonsun-gu, Suwon, Gyeonggido 441-813, Republic of Korea.
authors: Seon-Mi
authors: SM
authors: Seo
authors: Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnamdo 660-701, Republic of Korea.
authors: Junheon
authors: J
authors: Kim
authors: Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea.
authors: Jaesoon
authors: J
authors: Kang
authors: Division of Forest Insect Pests and Diseases, Korea Forest Research Institute, Seoul 130-712, Republic of Korea.
authors: Sang-Hyun
authors: SH
authors: Koh
authors: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors: Young-Joon
authors: YJ
authors: Ahn
authors: Department of Forest Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors: Kyu-Suk
authors: KS
authors: Kang
authors: Department of Forest Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: parkik1@snu.ac.kr.
authors: Il-Kwon
authors: IK
authors: Park
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D004789:Enzyme Activation / Q000187:drug effects; D005651:Fumigation; D008401:Gas Chromatography-Mass Spectrometry; D020049:Isoptera / Q000187:drug effects*; D009822:Oils, Volatile / Q000494:pharmacology*
chemical_list: D009822:Oils, Volatile; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase inhibition; Asteraceae plant essential oils; Chamaemelum nobile; Fumigant toxicity; Japanese termite; Santolina chamaecyparissus