title: Production and evaluation of recombinant granule antigen protein GRA7 for serodiagnosis of Toxoplasma gondii infection in cattle.
abstract: The precise detection of Toxoplasma gondii infection in cattle has important public health significance. In the present study, recombinant granule antigen protein GRA7 was evaluated as a potential diagnostic marker for T. gondii infection in cattle by an indirect enzyme-linked immunosorbent assay (ELISA) using the reference serum samples determined by immunofluorescence assay and Western blotting, showing that GRA7-ELISA has a sensitivity of 96.4%, and a specificity of 98.6%. The detection results by GRA7- and Toxoplasma lysate antigen-based ELISAs were compared, indicating that no significant difference (p>0.05) and perfect agreement (κ=0.74) was observed between the two tests. Receiver operating characteristic analysis showed a relative sensitivity and specificity of 100% and 97.3% at the cut-off value of 0.3 for GRA7-ELISA. These data demonstrate that GRA7 is a promising serodiagnostic marker for T. gondii infection in cattle.
authors: 1 Military Veterinary Institute , Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, China .
authors: Zedong
authors: Z
authors: Wang
authors: Wei
authors: W
authors: Ge
authors: Jiping
authors: J
authors: Li
authors: Mingxing
authors: M
authors: Song
authors: Hongchao
authors: H
authors: Sun
authors: Feng
authors: F
authors: Wei
authors: Quan
authors: Q
authors: Liu
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D012372:ROC Curve; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015415:Biomarkers; C112573:GRA7 protein, Toxoplasma gondii; D015800:Protozoan Proteins; D011994:Recombinant Proteins