title: Pyruvate kinase M2 in blood circulation facilitates tumor growth by promoting angiogenesis.
abstract: It is long known that pyruvate kinase isoform M2 (PKM2) is released into the circulation of cancer patients. The PKM2 levels in patients have been suggested as a diagnostic marker for many types of cancers. However, it is not known how PKM2 is released in the blood, and whether the circulating PKM2 has any physiological function(s) in tumor progression. In this report, we demonstrate that PKM2 in the blood facilitates tumor growth by promoting tumor angiogenesis. Our experiments show that PKM2 promotes tumor angiogenesis by increasing endothelial cell proliferation, migration, and cell-ECM adhesion. Only the dimeric PKM2 possess the activity in promoting tumor angiogenesis, which is consistent with the observations that PKM2 in circulation of cancer patients is a dimer form.
authors: From the Department of Biology and.
authors: Liangwei
authors: L
authors: Li
authors: From the Department of Biology and.
authors: Yinwei
authors: Y
authors: Zhang
authors: Department of Chemistry, Georgia State University, Atlanta, Georgia 30303.
authors: Jingjuan
authors: J
authors: Qiao
authors: Department of Chemistry, Georgia State University, Atlanta, Georgia 30303.
authors: Jenny J
authors: JJ
authors: Yang
authors: From the Department of Biology and zliu8@gsu.edu.
authors: Zhi-Ren
authors: ZR
authors: Liu
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D006019:Glycolysis / Q000235:genetics; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008565:Membrane Proteins / Q000097:blood*; D051379:Mice; D009369:Neoplasms / Q000097:blood / Q000473:pathology; D009360:Neoplastic Cells, Circulating / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000097:blood / Q000473:pathology*; D020033:Protein Isoforms / Q000097:blood*; D013963:Thyroid Hormones / Q000097:blood*; D023041:Xenograft Model Antitumor Assays; D000097810:Thyroid Hormone-Binding Proteins
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D020033:Protein Isoforms; D013963:Thyroid Hormones; D000097810:Thyroid Hormone-Binding Proteins
keywords: Angiogenesis; Cell Adhesion; Cell Migration; Endothelial Cell; Glycolysis; Pyruvate Kinase