title: Assessment of the implantation of day-2 human embryos by morphometric nonsubjective parameters.
abstract: OBJECTIVE
  To demonstrate the usefulness of image analysis in designing objective embryonic morphometric variables.
  
  
  METHODS
  Retrospective study of 214 top-quality day-2 embryo photographs from 50 double-embryo transfers resulting in no pregnancy (group 0) and 57 resulting in twin pregnancy (group 1).
  
  
  METHODS
  Human reproduction unit.
  
  
  METHODS
  Study of 107 in vitro fertilization-intracytoplasmic sperm injection (IVF-ICSI) cycles in women age <36 years with double-embryo transfer of top-quality embryos. Only the first cycle of IVF-ICSI was included.
  
  
  METHODS
  Standard IVF-ICSI protocols.
  
  
  METHODS
  The embryo photographs were analyzed using the ImageJ program. The effects of the embryo variables and the clinical variables on embryo implantation were evaluated using a stepwise dichotomous logistic regression.
  
  
  RESULTS
  Significant differences were observed, owing to the women's ages, internal perimeter, roundness factor, and zona pellucida thickness. Embryos with smaller internal perimeter, circular shape, and thinner zona pellucida were more likely to implant.
  
  
  CONCLUSIONS
  Morphometric variables lower the subjectivity of the current embryo grading systems. These variables are nonsubjective factors to consider when predicting implantation. Embryo image analysis is an accurate tool that can improve IVF-ICSI outcomes and reduce the number of twin pregnancies.
authors: Hospital Universitari i Politecnic La Fe, Valencia, Spain. Electronic address: mamobo1@dca.upv.es.
authors: Inmaculada
authors: I
authors: Molina
authors: Hospital Universitari i Politecnic La Fe, Valencia, Spain.
authors: Juan V
authors: JV
authors: Martínez
authors: Universitat de València, Valencia, Spain.
authors: José F
authors: JF
authors: Pertusa
authors: Universitat Politècnica de València, Valencia, Spain.
authors: Sebastian
authors: S
authors: Balasch
authors: Hospital Universitari i Politecnic La Fe, Valencia, Spain.
authors: Ignacio
authors: I
authors: Iniesta
authors: Hospital Universitari i Politecnic La Fe, Valencia, Spain.
authors: Antonio
authors: A
authors: Pellicer
mesh_terms: D000328:Adult; D000367:Age Factors; D010064:Embryo Implantation; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D016015:Logistic Models; D016017:Odds Ratio; D011237:Predictive Value of Tests; D011247:Pregnancy; D059285:Pregnancy, Twin; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013997:Time Factors
keywords: Embryo selection; embryo grading systems; embryo implantation; embryo score; images analysis; morphological and morphometric embryo variables