title: Immobilization of gold nanoparticles on cell culture surfaces for safe and enhanced gold nanoparticle-mediated laser transfection.
abstract: In comparison to standard transfection methods, gold nanoparticle-mediated laser transfection has proven to be a versatile alternative. This is based on its minor influence on cell viability and its high efficiency, especially for the delivery of small molecules like small interfering RNA. However, in order to transfer it to routine usage, a safety aspect is of major concern: The avoidance of nanoparticle uptake by the cells is desired. The immobilization of the gold nanoparticles on cell culture surfaces can address this issue. In this study, we achieved this by silanization of the appropriate surfaces and the binding of gold nanoparticles to them. Comparable perforation efficiencies to the previous approaches of gold nanoparticle-mediated laser transfection with free gold nanoparticles are demonstrated. The uptake of the immobilized particles by the cells is unlikely. Consequently, these investigations offer the possibility of bringing gold nanoparticle-mediated laser transfection closer to routine usage.
authors: Laser Zentrum Hannover e.V., Department of Biomedical Optics, 30419 Hannover, Germany.
authors: Stefan
authors: S
authors: Kalies
authors: Laser Zentrum Hannover e.V., Department of Biomedical Optics, 30419 Hannover, Germany.
authors: Dag
authors: D
authors: Heinemann
authors: Laser Zentrum Hannover e.V., Department of Biomedical Optics, 30419 Hannover, Germany.
authors: Markus
authors: M
authors: Schomaker
authors: Laser Zentrum Hannover e.V., Department of Biomedical Optics, 30419 Hannover, Germany.
authors: Lara
authors: L
authors: Gentemann
authors: Laser Zentrum Hannover e.V., Department of Biomedical Optics, 30419 Hannover, GermanybHannover Medical School, Department of Cardiothoracic, Transplantation and Vascular Surgery, 30625 Hannover, Germany.
authors: Heiko
authors: H
authors: Meyer
authors: Laser Zentrum Hannover e.V., Department of Biomedical Optics, 30419 Hannover, Germany.
authors: Tammo
authors: T
authors: Ripken
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D002470:Cell Survival; D004285:Dogs; D006046:Gold / Q000737:chemistry*; D007834:Lasers; D053768:Metal Nanoparticles / Q000737:chemistry*; D013499:Surface Properties; D014162:Transfection / Q000379:methods*
chemical_list: D006046:Gold