title: Biomolecule-to-fluorescent-color encoder: modulation of fluorescence emission via DNA structural changes.
abstract: A biomolecule-to-fluorescent-color (B/F) encoder for optical readout of biomolecular information is proposed. In the B/F encoder, a set of fluorescence wavelengths and their intensity levels are used for coding of a biomolecular signal. A hybridization chain reaction of hairpin DNAs labeled with fluorescent reporters was performed to generate the fluorescence color codes. The fluorescence is modulated via fluorescence resonance energy transfer, which is controlled by DNA structural changes. The results demonstrate that fluorescent color codes can be configured based on two wavelengths and five intensities using the B/F encoder, and the assigned codes can be retrieved via fluorescence measurements.
authors: Graduate School of Medicine, Osaka University, Suita, Osaka, 5650871, Japan.
authors: Takahiro
authors: T
authors: Nishimura
authors: Graduate School of Information Science and Technology, Osaka University, Suita, Osaka, 5650871, Japan.
authors: Yusuke
authors: Y
authors: Ogura
authors: Graduate School of Medicine, Osaka University, Suita, Osaka, 5650871, Japan.
authors: Kenji
authors: K
authors: Yamada
authors: Graduate School of Medicine, Osaka University, Suita, Osaka, 5650871, Japan.
authors: Yuko
authors: Y
authors: Ohno
authors: Graduate School of Information Science and Technology, Osaka University, Suita, Osaka, 5650871, Japan.
authors: Jun
authors: J
authors: Tanida
keywords: (170.0170) Medical optics and biotechnology; (280.1415) Biological sensing and sensors