title: A Huge PVDF Adsorption Difference Between Resveratrol and ε-Viniferin Allows to Quantitatively Purify Them and to Assess Their Anti-Tyrosinase Property.
abstract: Repeated chromatographic analyses of polyphenolic vine stalks extracts allowed us to note a huge adsorption difference on polyvinylidene fluoride (PVDF), betweentrans-resveratrol1and (+)-trans-ε-viniferin2. We could optimize the conditions (solvent, saturation of the process), for this polymer to adsorb very selectively2, with regard to the monomer1that remains in solution. Since membrane filters made of PVDF are quite often used for HPLC samples filtration, this observation prompted us to inform phytochemists studying plant stilbenoid contents. Based on this background information, we developed a straightforward and inexpensive enrichment process for either1and/or2, from crudeVitis viniferastalks extracts, allowing to get them in a pure form. Having at hand large amounts of these two pure compounds, they were tested and compared to a set of other relevant molecules for some biological properties:trans-ε-viniferin2was shown to be the most powerful tyrosinase inhibitor, among all samples tested.
authors: Faculty of Pharmacy, Laboratory of Pharmacognosy, Caudalie R&D, 15 Av. Charles Flahault, 34093 Montpellier, France.
authors: Cécile
authors: C
authors: Morel-Salmi
authors: Faculty of Pharmacy, Laboratory of Pharmacognosy, Caudalie R&D, 15 Av. Charles Flahault, 34093 Montpellier, France.
authors: Audrey
authors: A
authors: Julia
authors: Faculty of Pharmacy, Laboratory of Pharmacognosy, Institute of Biomolecules Max Mousseron (IBMM) (UMR 5247-CNRS-UM1-UM2-ENSCM), 15 Av. Charles Flahault, 34093 Montpellier, France.
authors: Claire
authors: C
authors: Vigor
authors: Faculty of Pharmacy, Laboratory of Pharmacognosy, Institute of Biomolecules Max Mousseron (IBMM) (UMR 5247-CNRS-UM1-UM2-ENSCM), 15 Av. Charles Flahault, 34093 Montpellier, France.
authors: Joseph
authors: J
authors: Vercauteren
keywords: Polyvinylidene fluoride (PVDF); Tyrosinase inhibition; Vitis vinifera; trans-Epsilon-viniferin; trans-Resveratrol