title: Alteration of thyroid hormone levels and related gene expression in Chinese rare minnow larvae exposed to mercury chloride.
abstract: Mercury is a prominent environmental contaminant that causes endocrine disorder to human and other organisms. But little is known about the response of the thyroid functions and hypothalamic-pituitary-thyroid (HPT) axis to mercury in teleosts and the few studies that are available have not yielded consistent results. In this study, expression profiles of corticotropin-releasing hormone (crh), thyroid stimulating hormone beta (tshβ), solute carrier family 5 (sodium iodide symporter) member 5 (slc5a5), thyroglobulin (tg), thyroid hormone receptor alpha (trα) and thyroid hormone receptor beta (trβ) genes were determined in whole-body of Chinese rare minnow (Gobiocypris rarus) larvae after exposure to different levels of Hg(2+) (0, 0.1 and 0.3 mg/l) for 4 days, as well as the thyroid hormones (THs) levels. Moreover, the 96-h lethal concentration of Hg(2+) on rare minnow larvae was determined as 0.32 mg/l. The results showed that crh, tg, trα and trβ mRNA levels were significantly up-regulated in the larvae, but the gene expression of tshβ and slc5a5 was not significantly changed in our study. Besides, the THs levels increased in the whole-body of fish, especially the thyroxine (T4) level. The above results indicated that Hg(2+) could alter some genes expression in the HPT axis which could be used as the potential biomarkers for evaluating the environmental Hg(2+)-induced stress in fish.
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: zhihuali06@yahoo.com.
authors: Zhi-Hua
authors: ZH
authors: Li
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
authors: Lu
authors: L
authors: Chen
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China.
authors: Yan-Hua
authors: YH
authors: Wu
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
authors: Ping
authors: P
authors: Li
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
authors: Yun-Feng
authors: YF
authors: Li
authors: Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. Electronic address: nizhaoh@yfi.ac.cn.
authors: Zhao-Hui
authors: ZH
authors: Ni
mesh_terms: D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000235:genetics; D003530:Cyprinidae / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D007928:Lethal Dose 50; D008627:Mercuric Chloride / Q000633:toxicity*; D027981:Symporters / Q000235:genetics; D013954:Thyroglobulin / Q000235:genetics; D037021:Thyroid Hormone Receptors alpha / Q000235:genetics; D037042:Thyroid Hormone Receptors beta / Q000235:genetics; D037322:Thyrotropin, beta Subunit / Q000235:genetics; D013974:Thyroxine / Q000378:metabolism*; D014284:Triiodothyronine / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000099209:Sodium-Iodide Symporters
chemical_list: D003346:Corticotropin-Releasing Hormone; D008627:Mercuric Chloride; D027981:Symporters; D013954:Thyroglobulin; D037021:Thyroid Hormone Receptors alpha; D037042:Thyroid Hormone Receptors beta; D037322:Thyrotropin, beta Subunit; D013974:Thyroxine; D014284:Triiodothyronine; D014874:Water Pollutants, Chemical; D000099209:Sodium-Iodide Symporters
keywords: Chinese rare minnow; Gene expression; Hypothalamic–pituitary–thyroid axis; Mercury; Thyroid hormone