title: Proteomic and biochemical basis for enhanced growth yield of Enterobacter sp. LCR1 on insoluble phosphate medium.
abstract: Proteomics and biochemical analyses were used to unravel the basis for higher growth yield of Enterobacter sp. LCR1 on insoluble phosphate medium compared to soluble. Proteomic analysis using 2-DE, MALDI-TOF/MS and LC-MS revealed the involvement of nine proteins. Down-regulation of fructose bisphosphate aldolase with decreased concentrations of glucose-6-phosphate and fructose-6-phosphate indicated diminished glycolysis. However, up-regulation of phosphoglycerate mutase, increase in the activities of 6-phosphogluconate dehydratase, 2-keto-3-deoxy-6-phosphogluconate aldolase and 6-phosphogluconate dehydrogenase suggested induction of Entner-Doudoroff and pentose phosphate pathways. These pathways generate sufficient energy from gluconic acid, which is also used for biosynthesis as indicated by up-regulation of elongation factor Tu, elongation factor G and protein disulfide isomerase. Increased reactive oxygen species (ROS) formation resulting from organic acid oxidation leads to overexpressed manganese superoxide dismutase and increased activities of catalase and ascorbate peroxidase. Thus the organism uses gluconate instead of glucose for energy, while alleviating extra ROS formation by oxidative defense enzymes.
authors: Molecular Biology Section, Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India; Department of Biotechnology and Microbiology, Saaii College of Medical Science and Technology, Chaubeypur, Kanpur, India. Electronic address: arvindbhu7@gmail.com.
authors: Arvind
authors: A
authors: Kumar
authors: Molecular Biology Section, Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors: Lal Chand
authors: LC
authors: Rai
mesh_terms: D003470:Culture Media; D004734:Energy Metabolism; D004754:Enterobacter / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D055442:Metabolome; D055432:Metabolomics; D010084:Oxidation-Reduction; D010710:Phosphates / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics
chemical_list: D003470:Culture Media; D010710:Phosphates; D020543:Proteome
keywords: 2-DE; Enterobacter sp.; MALDI-TOF/MS; P solubilization; RT-PCR