title: Cellular metabolic, stress, and histological response on exposure to acute toxicity of endosulfan in tilapia (Oreochromis mossambicus).
abstract: Endosulfan is one of the most hazardous organochlorines pesticides responsible for environmental pollution, as it is very persistent and shows bio-magnification. This study evaluated the impact of acute endosulfan toxicity on metabolic enzymes, lysozyme activities, heat shock protein (Hsp) 70 expression, and histopathology in Tilapia (Oreochromis mossambicus). Among the indicators that were induced in dose dependent manner were the enzymes of amino acid metabolism (serum alanine aminotransferase and aspartate aminotransferase), carbohydrate metabolism (serum lactate dehydrogenase), pentose phosphate pathway (Glucose-6-phosphate dehydrogenase) as well as lysozyme and Hsp70 in liver and gill, while liver and gill Isocitrate dehydrogenase (TCA cycle enzyme) and marker of general energetics (Total adenosine triphosphatase) were inhibited. Histopathological alterations in gill were clubbing of secondary gill lamellae, marked hyperplasia, complete loss of secondary lamellae and atrophy of primary gill filaments. Whereas in liver, swollen hepatocyte, and degeneration with loss of cellular boundaries were distinctly noticed. Overall results clearly demonstrated the unbalanced metabolism and damage of the vital organs like liver and gill in Tilapia due to acute endosulfan exposure.
authors: Central Institute of Fisheries Education, Versova, Mumbai, 400061, Maharashtra, India.|Edaphic Stress Management, National Institute of Abiotic Stress Management, Baramati, Pune, 413115, Maharashtra, India.
authors: Neeraj
authors: N
authors: Kumar
authors: Central Institute of Fisheries Education, Versova, Mumbai, 400061, Maharashtra, India.
authors: Rupam
authors: R
authors: Sharma
authors: Central Institute of Fisheries Education, Versova, Mumbai, 400061, Maharashtra, India.
authors: Gayatri
authors: G
authors: Tripathi
authors: Central Institute of Fisheries Education, Versova, Mumbai, 400061, Maharashtra, India.
authors: Kundan
authors: K
authors: Kumar
authors: Central Institute of Fisheries Education, Versova, Mumbai, 400061, Maharashtra, India.|Department of Zoology, Maharshi Dayanand College, Parel, Mumbai, 400012, Maharashtra, India.
authors: Rishikesh S
authors: RS
authors: Dalvi
authors: Central Institute of Fisheries Education, Versova, Mumbai, 400061, Maharashtra, India.
authors: Gopal
authors: G
authors: Krishna
mesh_terms: D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood / Q000378:metabolism; D004726:Endosulfan / Q000633:toxicity*; D005880:Gills / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000097:blood / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009113:Muramidase / Q000097:blood / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects; D017210:Tilapia / Q000378:metabolism*; D023382:Toxicity Tests, Acute
chemical_list: D018840:HSP70 Heat-Shock Proteins; D007306:Insecticides; D005954:Glucosephosphate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D009113:Muramidase; D004726:Endosulfan
keywords: Hsp70; Tilapia; biochemical parameters; endosulfan; histopathology; lysozyme activity