title: Changes in TcpA gene frequency explain 2,4,6-trichlorophenol degradation in mesocosms.
abstract: Soils are often polluted by chlorophenols in timber production areas in the northern hemisphere. The tcpA gene encodes the first step of 2,4,6-trichlorophenol (246-TCP) degradation. We tested tcpA gene frequency in three natural pristine soils with different 246-TCP degradation capacity. Gene tcpA frequency increased more in spiked than non-spiked 10-L pails containing coniferous humus soil with high degradation capacity, in contrast to soils where degradation was slower. As the soil in each mesocosm originated from a spatially separate field plot, changes in tcpA gene frequency affected 246-TCP degradation over a range of soil origins. This indicates that the abundance of and changes in tcpA gene frequency could be utilized in estimating the efficacy of natural attenuation and biostimulation treatments in controlled conditions.
authors: a University of Helsinki , Department of Environmental Sciences , Lahti , Finland.
authors: Aki
authors: A
authors: Sinkkonen
authors: Sara
authors: S
authors: Ollila
authors: Martin
authors: M
authors: Romantschuk
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000032:analysis / Q000378:metabolism*; D005787:Gene Frequency; D005819:Genetic Markers; D006899:Mixed Function Oxygenases / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002733:Chlorophenols; D005819:Genetic Markers; D012989:Soil Pollutants; C484322:2,4,6-trichlorophenol monooxygenase; D006899:Mixed Function Oxygenases; C024564:2,4,6-trichlorophenol
keywords: 2; 4; 6-trichlorophenol; coniferous humus; gene frequency; mineral soil; natural attenuation; peat; primer; pristine soil; tcpA degrader gene