title: A portable lipid bilayer system for environmental sensing with a transmembrane protein.
abstract: This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor.
authors: Artificial Cell Membrane Systems Group, Kanagawa Academy of Science and Technology (KAST), Takatsu-ku, Kawasaki, Japan.
authors: Ryuji
authors: R
authors: Kawano
authors: Artificial Cell Membrane Systems Group, Kanagawa Academy of Science and Technology (KAST), Takatsu-ku, Kawasaki, Japan; Department of Mechanical Engineering, Keio University, Kohoku-ku, Yokohama, Japan.
authors: Yutaro
authors: Y
authors: Tsuji
authors: Artificial Cell Membrane Systems Group, Kanagawa Academy of Science and Technology (KAST), Takatsu-ku, Kawasaki, Japan.
authors: Koki
authors: K
authors: Kamiya
authors: Artificial Cell Membrane Systems Group, Kanagawa Academy of Science and Technology (KAST), Takatsu-ku, Kawasaki, Japan; Department of Mechanical Engineering, Keio University, Kohoku-ku, Yokohama, Japan.
authors: Taiga
authors: T
authors: Kodama
authors: Artificial Cell Membrane Systems Group, Kanagawa Academy of Science and Technology (KAST), Takatsu-ku, Kawasaki, Japan; CIRMM-IIS, The University of Tokyo, Meguro-ku, Tokyo, Japan.
authors: Toshihisa
authors: T
authors: Osaki
authors: Artificial Cell Membrane Systems Group, Kanagawa Academy of Science and Technology (KAST), Takatsu-ku, Kawasaki, Japan; Department of Mechanical Engineering, Keio University, Kohoku-ku, Yokohama, Japan.
authors: Norihisa
authors: N
authors: Miki
authors: Artificial Cell Membrane Systems Group, Kanagawa Academy of Science and Technology (KAST), Takatsu-ku, Kawasaki, Japan; CIRMM-IIS, The University of Tokyo, Meguro-ku, Tokyo, Japan.
authors: Shoji
authors: S
authors: Takeuchi
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods*; D008051:Lipid Bilayers / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins