title: The sciatic nerve cuffing model of neuropathic pain in mice.
abstract: Neuropathic pain arises as a consequence of a lesion or a disease affecting the somatosensory system. This syndrome results from maladaptive changes in injured sensory neurons and along the entire nociceptive pathway within the central nervous system. It is usually chronic and challenging to treat. In order to study neuropathic pain and its treatments, different models have been developed in rodents. These models derive from known etiologies, thus reproducing peripheral nerve injuries, central injuries, and metabolic-, infectious- or chemotherapy-related neuropathies. Murine models of peripheral nerve injury often target the sciatic nerve which is easy to access and allows nociceptive tests on the hind paw. These models rely on a compression and/or a section. Here, the detailed surgery procedure for the "cuff model" of neuropathic pain in mice is described. In this model, a cuff of PE-20 polyethylene tubing of standardized length (2 mm) is unilaterally implanted around the main branch of the sciatic nerve. It induces a long-lasting mechanical allodynia, i.e., a nociceptive response to a normally non-nociceptive stimulus that can be evaluated by using von Frey filaments. Besides the detailed surgery and testing procedures, the interest of this model for the study of neuropathic pain mechanism, for the study of neuropathic pain sensory and anxiodepressive aspects, and for the study of neuropathic pain treatments are also discussed.
authors: Institut des Neurosciences Cellulaires et Intégratives UPR3212, Centre National de la Recherche Scientifique; yalcin@inci-cnrs.unistra.fr.
authors: Ipek
authors: I
authors: Yalcin
authors: Institut des Neurosciences Cellulaires et Intégratives UPR3212, Centre National de la Recherche Scientifique; Université de Strasbourg.
authors: Salim
authors: S
authors: Megat
authors: Institut des Neurosciences Cellulaires et Intégratives UPR3212, Centre National de la Recherche Scientifique; Université de Strasbourg.
authors: Florent
authors: F
authors: Barthas
authors: Institut des Neurosciences Cellulaires et Intégratives UPR3212, Centre National de la Recherche Scientifique.
authors: Elisabeth
authors: E
authors: Waltisperger
authors: Institut des Neurosciences Cellulaires et Intégratives UPR3212, Centre National de la Recherche Scientifique; Université de Strasbourg.
authors: Mélanie
authors: M
authors: Kremer
authors: Institut des Neurosciences Cellulaires et Intégratives UPR3212, Centre National de la Recherche Scientifique; Université de Strasbourg; Hôpitaux Universitaires de Strasbourg.
authors: Eric
authors: E
authors: Salvat
authors: Institut des Neurosciences Cellulaires et Intégratives UPR3212, Centre National de la Recherche Scientifique.
authors: Michel
authors: M
authors: Barrot
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006930:Hyperalgesia / Q000209:etiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009437:Neuralgia / Q000209:etiology*; D012584:Sciatic Nerve / Q000503:physiopathology* / Q000601:surgery*; D020426:Sciatic Neuropathy / Q000209:etiology / Q000503:physiopathology