title: Molecular and serologic detection of Coxiella burnetii in native Korean goats (Capra hircus coreanae).
abstract: The occurrence of Q fever in native Korean goats (Capra hircus coreanae) was investigated for the first time in the country using ELISA and PCR. A total of 597 blood samples were collected from goats belonging to five different provinces of Korea. To detect Coxiella burnetii, sera were separated from the whole blood and analysed by ELISA; DNA was extracted directly from the whole blood and analysed by PCR. Overall, 114 (19.1%, 95% C.I.=16.1-22.4) and 57 goats (9.5%, 95% C.I.=7.5-12.2) tested positive for C. burnetii in the ELISA- and PCR-based screening, respectively, while 18 goats (3.0%, 95% C.I.=1.9-4.7) tested positive in both the assays. There was a significant difference between the number of ELISA- and PCR-positive goats (P<0.05). The seroprevalence of Q fever was significantly higher among the adult goats (≥1y, 22.0%) than among the young goats (<1y, 13.8%) (P<0.05). While the results of the serologic analysis showed no seasonal variation, data from the PCR-based assay indicated that there were a higher number of positive cases during the cold seasons. Because Q fever infection has high rates of prevalence in native Korean goats, further studies on humans at a high risk of contracting this disease should be conducted. The PCR-based assay used in this study is a useful method for the direct detection of C. burnetii in blood samples from small ruminants.
authors: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Anyang, South Korea.
authors: Byeong Yeal
authors: BY
authors: Jung
authors: College of Veterinary Medicine and Cardiovascular Research Institute, Kyungpook National University, Daegu, South Korea; Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, South Korea.
authors: Min-Goo
authors: MG
authors: Seo
authors: College of Veterinary Medicine and Cardiovascular Research Institute, Kyungpook National University, Daegu, South Korea.
authors: Seung-Hun
authors: SH
authors: Lee
authors: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Anyang, South Korea.
authors: Jae-Won
authors: JW
authors: Byun
authors: Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Anyang, South Korea.
authors: Jae-Ku
authors: JK
authors: Oem
authors: College of Veterinary Medicine and Cardiovascular Research Institute, Kyungpook National University, Daegu, South Korea. Electronic address: dmkwak@knu.ac.kr.
authors: Dongmi
authors: D
authors: Kwak
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D016997:Coxiella burnetii / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015511:Goat Diseases / Q000453:epidemiology* / Q000821:virology; D006041:Goats; D006801:Humans; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D011778:Q Fever / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D056910:Republic of Korea / Q000453:epidemiology; D016036:Seroepidemiologic Studies
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial
keywords: ELISA; Native Korean goat; PCR; Q fever