title: Novel RNA recognition motif domain in the cytoplasmic polyadenylation element binding protein 3.
abstract: The family of cytoplasmic polyadenylation element binding proteins CPEB1, CPEB2, CPEB3, and CPEB4 binds to the 3'-untranslated region (3'-UTR) of mRNA, and plays significant roles in mRNA metabolism and translation regulation. They have a common domain organization, involving two consecutive RNA recognition motif (RRM) domains followed by a zinc finger domain in the C-terminal region. We solved the solution structure of the first RRM domain (RRM1) of human CPEB3, which revealed that CPEB3 RRM1 exhibits structural features distinct from those of the canonical RRM domain. Our structural data provide important information about the RNA binding ability of CPEB3 RRM1.
authors: RIKEN Systems and Structural Biology Center, Tsurumi-ku, Yokohama, 230-0045, Japan; Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Tsurumi-ku, Yokohama, 230-0045, Japan.
authors: Kengo
authors: K
authors: Tsuda
authors: Kanako
authors: K
authors: Kuwasako
authors: Takashi
authors: T
authors: Nagata
authors: Mari
authors: M
authors: Takahashi
authors: Takanori
authors: T
authors: Kigawa
authors: Naohiro
authors: N
authors: Kobayashi
authors: Peter
authors: P
authors: Güntert
authors: Mikako
authors: M
authors: Shirouzu
authors: Shigeyuki
authors: S
authors: Yokoyama
authors: Yutaka
authors: Y
authors: Muto
mesh_terms: D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D030562:Databases, Protein; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055550:Protein Stability; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D012995:Solubility
chemical_list: D020413:3' Untranslated Regions; C513866:CPEB3 protein, human; D010446:Peptide Fragments; D020033:Protein Isoforms; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins
keywords: 3′-UTR; CPE; CPEB3; GluR2; Mrna; NMR; RRM; structure