title: Competitive assembly to increase the performance of the DNA/carbon-nanomaterial-based sensing platform.
abstract: Increasing the rate of target binding on the surface and enhancing the fluorescence signal restoration efficiency are critical to the desirable biomedical application of carbon nanomaterials, for example, single-walled carbon nanotubes (SWNTs). We describe here a strategy to increase the target binding rate and enhance the fluorescence signal restoration efficiency on the DNA-functionalized SWNT surface using a short complementary DNA (scDNA) strand. The scDNA causes up to a 2.5-fold increase in association rate and 4-fold increase in fluorescence signal restoration by its competitive assembly on the nanostructure's surface and inducing a conformational change that extends the DNA away from the surface, making it more available to bind target nucleic acids. The scDNA-induced enhancement of binding kinetics and fluorescence signal restoration efficiency is a general phenomenon that occurred with all sequences and surfaces investigated. Through this competitive assembly strategy of scDNA, performance improvement of the carbon-nanomaterial-based biosensing platform for both in vitro detection and live cell imaging can be reached.
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors: Qiao
authors: Q
authors: Tang
authors: Qier
authors: Q
authors: Zhang
authors: Ying
authors: Y
authors: Jiang
authors: Jishan
authors: J
authors: Li
authors: Jing
authors: J
authors: Zheng
authors: Yinhui
authors: Y
authors: Li
authors: Ronghua
authors: R
authors: Yang
authors: Weihong
authors: W
authors: Tan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D018076:DNA, Complementary / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008027:Light; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010316:Particle Size; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence
chemical_list: D018076:DNA, Complementary; D037742:Nanotubes, Carbon; D004247:DNA