title: Precursor ion scan driven fast untargeted screening and semi-determination of caffeoylquinic acid derivatives in Cynara scolymus L.
abstract: A precursor ion scan (PIS) technique based strategy was developed for rapid screening and semi-determination of caffeoylquinic acid derivatives (CADs) in artichoke (Cynara scolymus L.) using ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry. 1,5-Dicaffeoylquinic acid and 5-caffeoylquinic acid were used for studying the fragmentation behaviour of two classes of CADs, setting m/z 191 as a diagnostic moiety. When it was applied to artichoke sample, ten CADs were detected and elucidated in a single PIS run. Furthermore, method validation was implemented including: specificity (no interference), linearity (≥0.9993), limit of detection (LOD<0.12 ng mL(-1)) and limit of quantification (LOQ<0.25 ng mL(-1)), precision (RSD≤3.6), recovery (91.4-95.9%) and stability (at least 12 h). This approach was proven to be a powerful, selective and sensitive tool for rapid screening and semi-determination of untargeted components in natural products.
authors: College of Food Science and Technology, Zhejiang Gongshang University, Hangzhou, China; Department of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region.
authors: Qing
authors: Q
authors: Shen
authors: College of Food Science and Technology, Zhejiang Gongshang University, Hangzhou, China.
authors: Yanbin
authors: Y
authors: Lu
authors: College of Food Science and Technology, Zhejiang Gongshang University, Hangzhou, China. Electronic address: dzy@zjgsu.edu.cn.
authors: Zhiyuan
authors: Z
authors: Dai
authors: Department of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region. Electronic address: bhhonyun@cityu.edu.hk.
authors: Hon-Yeung
authors: HY
authors: Cheung
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D031943:Cynara scolymus / Q000737:chemistry*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C472707:caffeoylquinic acid; D011801:Quinic Acid
keywords: Caffeoylquinic acid derivative; Cynara scolymus L.; Precursor ion scan; UPLC–MS/MS