title: CD147 up-regulates calcium-induced chemotaxis, adhesion ability and invasiveness of human neutrophils via a TRPM-7-mediated mechanism.
abstract: OBJECTIVE
  We aimed to investigate whether CD147 can up-regulate the chemotactic, adhesive and invasive properties of human neutrophils and to determine the mechanism underlying this process.
  
  
  METHODS
  Human promyelocytic leukaemia cells (HL-60) cells and peripheral blood or synovial fluid neutrophils were isolated from RA patients. Under cyclophilin A (CypA) stimulation, chemotaxis, adhesion potential and invasion ability were assessed using chemotaxis, adhesion and invasiveness assays. Lipid raft isolation and western blot were used to determine the mechanism underlying the effects of CypA stimulation.
  
  
  RESULTS
  CD147 up-regulates the calcium-induced chemotaxis, adhesion ability and invasiveness of human neutrophils in RA patients. Transient receptor potential melastatin 7 may be responsible for this phenomenon.
  
  
  CONCLUSIONS
  These findings suggest that in RA patients, abundant CypA up-regulates the calcium-induced chemotactic, adhesive and invasive properties of neutrophils via direct binding to CD147. Cyclophilin-CD147 interactions might contribute to the destruction of cartilage and bone in RA.
authors: Department of Clinical Immunology, Xijing Hospital and Department of Cell Biology/Cell Engineering Research Centre, Fourth Military Medical University, Xi'an, China.
authors: Cong-Hua
authors: CH
authors: Wang
authors: Department of Clinical Immunology, Xijing Hospital and Department of Cell Biology/Cell Engineering Research Centre, Fourth Military Medical University, Xi'an, China.
authors: Meng-Yao
authors: MY
authors: Rong
authors: Department of Clinical Immunology, Xijing Hospital and Department of Cell Biology/Cell Engineering Research Centre, Fourth Military Medical University, Xi'an, China.
authors: Li
authors: L
authors: Wang
authors: Department of Clinical Immunology, Xijing Hospital and Department of Cell Biology/Cell Engineering Research Centre, Fourth Military Medical University, Xi'an, China.
authors: Zhen
authors: Z
authors: Ren
authors: Department of Clinical Immunology, Xijing Hospital and Department of Cell Biology/Cell Engineering Research Centre, Fourth Military Medical University, Xi'an, China.
authors: Li-Na
authors: LN
authors: Chen
authors: Department of Clinical Immunology, Xijing Hospital and Department of Cell Biology/Cell Engineering Research Centre, Fourth Military Medical University, Xi'an, China.
authors: Jun-Feng
authors: JF
authors: Jia
authors: Department of Clinical Immunology, Xijing Hospital and Department of Cell Biology/Cell Engineering Research Centre, Fourth Military Medical University, Xi'an, China.
authors: Xue-Yi
authors: XY
authors: Li
authors: Department of Clinical Immunology, Xijing Hospital and Department of Cell Biology/Cell Engineering Research Centre, Fourth Military Medical University, Xi'an, China.
authors: Zhen-Biao
authors: ZB
authors: Wu
authors: Department of Clinical Immunology, Xijing Hospital and Department of Cell Biology/Cell Engineering Research Centre, Fourth Military Medical University, Xi'an, China.
authors: Zhi-Nan
authors: ZN
authors: Chen
authors: Department of Clinical Immunology, Xijing Hospital and Department of Cell Biology/Cell Engineering Research Centre, Fourth Military Medical University, Xi'an, China. zhuping@fmmu.edu.cn.
authors: Ping
authors: P
authors: Zhu
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D051926:Basigin / Q000235:genetics / Q000276:immunology*; D002118:Calcium / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D008297:Male; D021962:Membrane Microdomains / Q000276:immunology; D008875:Middle Aged; D020556:Neutrophil Infiltration / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D017346:Protein Serine-Threonine Kinases; D034622:RNA Interference; D050053:TRPM Cation Channels / Q000235:genetics / Q000276:immunology*; D015854:Up-Regulation / Q000276:immunology; D055815:Young Adult
chemical_list: C497886:BSG protein, human; D050053:TRPM Cation Channels; D051926:Basigin; D017346:Protein Serine-Threonine Kinases; C486583:TRPM7 protein, human; D002118:Calcium
keywords: CD147; TRPM-7; calcium influx; neutrophil; rheumatoid arthritis