title: Phosphorylation of myofibrillogenesis regulator-1 activates the MAPK signaling pathway and induces proliferation and migration in human breast cancer MCF7 cells.
abstract: Myofibrillogenesis regulator-1 (MR-1) has been characterized as a tumor promoter in many cancers. However, its mechanism of action has not been fully elucidated. Here, we report that MR-1 is overexpressed in human breast cancer cells and participates in tumor promotion in human breast cancer MCF7 cells by activating the ERK1/2 signaling pathway. MR-1 interacts with MEK1/2 and ERK1, and its N-terminal sequence plays a major role in promoting the MEK/ERK cascade. Furthermore, six phosphorylation sites of MR-1 were identified, and phosphorylation at S46 was shown to be critical for the activation of MEK/ERK. Therefore, our findings suggest that MR-1 functions as a tumor promoter in MCF7 cells by activating the MEK/ERK signaling.
authors: Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Tiantan Xili, Beijing 100050, China.
authors: Yuyan
authors: Y
authors: Gong
authors: Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Tiantan Xili, Beijing 100050, China.
authors: Hongwei
authors: H
authors: He
authors: Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Tiantan Xili, Beijing 100050, China.
authors: Hong
authors: H
authors: Liu
authors: Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Tiantan Xili, Beijing 100050, China.
authors: Caixia
authors: C
authors: Zhang
authors: Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Tiantan Xili, Beijing 100050, China.
authors: Wuli
authors: W
authors: Zhao
authors: Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Tiantan Xili, Beijing 100050, China. Electronic address: shaor@imb.pumc.edu.cn.
authors: Rong-Guang
authors: RG
authors: Shao
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000473:pathology*; D063646:Carcinogenesis; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D020935:MAP Kinase Signaling System; D061986:MCF-7 Cells; D009124:Muscle Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D009124:Muscle Proteins; C495444:PNKD protein, human; D034741:RNA, Small Interfering
keywords: Breast cancer; ERK1; MEK1/2; Myofibrillogenesis regulator 1; Phosphorylation sites