title: Trilobatin attenuates the LPS-mediated inflammatory response by suppressing the NF-κB signaling pathway.
abstract: We investigated the anti-inflammatory effect of trilobatin, the flavonoid isolated from the leaves of Lithocarpus polystachyus Rehd, as well as the underlying molecular mechanisms. Treatment with trilobatin (0.005-5 μM) dose-dependently inhibited the lipopolysaccharide (LPS)-induced mRNA expression and secretion of pro-inflammatory cytokines, including tumor necrosis factor α (TNFα), interleukin-1β (IL-1β) and interleukin-6 (IL-6), in RAW 264.7 macrophages. However, no further inhibition was detected when the concentration of trilobatin was increased to 50 μM. Western blot analysis confirmed that the mechanism of the anti-inflammatory effect was correlated with the inhibition of LPS-induced inhibitor of nuclear factor-kappa B α (IκBα) degradation and nuclear factor-kappa B (NF-κB) p65 phosphorylation. In addition, trilobatin also showed a significant inhibition of LPS-induced TNFα and IL-6 at both the mRNA and protein levels in a mouse model. Our results suggest that trilobatin potentially inhibits the LPS-induced inflammatory response by suppressing the NF-κB signaling pathway.
authors: Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
authors: Xiaolong
authors: X
authors: Fan
authors: Department of Food Safety, College of Food and Horticultural Sciences, Foshan University (Northern Campus), Foshan 528231, China. Electronic address: zhang_ying-hui@163.com.
authors: Yinghui
authors: Y
authors: Zhang
authors: Department of Food Safety, College of Food and Horticultural Sciences, Foshan University (Northern Campus), Foshan 528231, China.
authors: Huaqiang
authors: H
authors: Dong
authors: Department of Veterinary Medicine, College of Life Science, Foshan University (Northern Campus), Foshan 528231, China.
authors: Bingyun
authors: B
authors: Wang
authors: Department of Veterinary Medicine, College of Life Science, Foshan University (Northern Campus), Foshan 528231, China.
authors: Huiqin
authors: H
authors: Ji
authors: Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address: liuxin@scau.edu.cn.
authors: Xin
authors: X
authors: Liu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D029962:Fagaceae / Q000737:chemistry; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D059808:Polyphenols / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D020672:I-kappa B Proteins; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; D059808:Polyphenols; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; C000598619:trilobatin
keywords: Anti-inflammation; NF-κB; Pro-inflammatory cytokines; Trilobatin