title: Effect of WNT5A on epithelial-mesenchymal transition and its correlation with tumor invasion and metastasis in nasopharyngeal carcinoma.
abstract: OBJECTIVE
  To investigate the correlation between nasopharyngeal carcinoma cell WNT5A and epithelial-mesenchymal transition (emt)/metastasis, and investigate its possible mechanisms.
  
  
  METHODS
  RT-PCR and gene transfection were used to detect the expression of nasopharyngeal carcinoma cell strains WNT5A and EMT related factor 5-8F. Transient transfection of NPC cell line 5-8F was determined by liposome of plasmid with WNT5A gene. The differential expressions of WNT5A and EMT-related factors in cells before and after transfection were detected by RT-PCR. Cell scratch assay and Transwell assay were used to detect the motility abilities of cells before and after 5-8F transfection.
  
  
  RESULTS
  The expressions of WNT5A and EMT related factors matrix metalloproteinase-2 of the WNT5A transferred group in the nasopharyngeal carcinoma cell line 5-8F were higher than the blank control group and the empty vector transferred group, and the transfer ability of the WNT5A transferred group was higher than that in the blank control group and the empty vector transferred group, while the expressions of EMT related factors E-cadherin were lower than that in the blank control group and the empty vector transferred group, and the transfer ability of the WNT5A transferred group was higher than that in the blank control group and the empty vector transferred group.
  
  
  CONCLUSIONS
  In nasopharyngeal carcinoma cells, WNT5A can regulate the epithelial-mesenchymal transition and affect the ability of tumor invasion and metastasis of nasopharyngeal carcinoma.
authors: Rhinology of the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
authors: Hong-Hai
authors: HH
authors: Zhu
authors: Department of Otolaryngology-Head and Neck Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
authors: Xiao-Yuan
authors: XY
authors: Zhu
authors: Rhinology of the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China. Electronic address: zmh0334@126.com.
authors: Ming-Hui
authors: MH
authors: Zhou
authors: Department of Nephrology of the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
authors: Gen-Yang
authors: GY
authors: Cheng
authors: Department of Otolaryngology-Head and Neck Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
authors: Wei-Hua
authors: WH
authors: Lou
mesh_terms: D015820:Cadherins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051153:Wnt Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000071818:Wnt-5a Protein
chemical_list: D015820:Cadherins; D011518:Proto-Oncogene Proteins; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; D020778:Matrix Metalloproteinase 2
keywords: E-cadherin; Epithelial-mesenchymal transition; Matrix metalloproteinase-2; Metastasis; Nasopharyngeal cancer; WNT5A