title: Simultaneous determination of aflatoxins B₁, B₂, G₁, G₂ in Fructus Bruceae by high-performance liquid chromatography with online postcolumn photochemical derivatization.
abstract: A simple, reliable, and highly sensitive method for the simultaneous determination of aflatoxin B1 , B2 , G1 , G2 in Fructus Bruceae was developed using high-performance liquid chromatography coupled to online postcolumn photochemical derivatization and fluorescence detection. Aflatoxins were first extracted by a methanol/water mixture and then cleaned up with an AflaTest™ immunoaffinity column. Different clean-up and derivatization methods were compared and optimized. The established method was extensively validated to show satisfactory performance of linearity (R(2) ≥ 0.9997), recovery (74.3-100.8%), and precision (RSDs ≤ 2.8%) for the investigated aflatoxins. This proposed method was also applied to 11 F. Bruceae samples and the results showed that 10 out of 11 were contaminated with aflatoxins ranging from 0.26 to 27.52 μg/kg and the occurrence of aflatoxin B1 , the most toxic one, was as high as 91% in all the samples, highlighting the severe contamination and the necessity to set legal limits for aflatoxins in F. Bruceae.
authors: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China; School of Pharmacy, Chengdu University of TCM, Chengdu, China.
authors: Jiliang
authors: J
authors: Cao
authors: Shujun
authors: S
authors: Zhou
authors: Weijun
authors: W
authors: Kong
authors: Xiaochi
authors: X
authors: Ma
authors: Meihua
authors: M
authors: Yang
authors: Li
authors: L
authors: Wan
authors: Shihai
authors: S
authors: Yang
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D000348:Aflatoxins / Q000032:analysis*; D000089003:Brucea javanica; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D055668:Photochemical Processes; D053719:Tandem Mass Spectrometry
chemical_list: D000348:Aflatoxins; C571445:Brucea javanica fruit extract; D004365:Drugs, Chinese Herbal; C027955:aflatoxin G1; C029754:aflatoxin G2; C029753:aflatoxin B2; D016604:Aflatoxin B1
keywords: Aflatoxins; Fluorescence detection; Fructus Bruceae; Photochemical derivatization