title: Deletion of the lmna gene induces growth delay and serum biochemical changes in C57BL/6 mice.
abstract: The A-type lamin deficient mouse line (Lmna (-/-) ) has become one of the most frequently used models for providing insights into many different aspects of A-type lamin function. To elucidate the function of Lmna in the growth and metabolism of mice, tissue growth and blood biochemistry were monitored in Lmna-deficient mice, heterozygous (Lmna (+/-) ) and wide-type (Lmna (+) (/) (+) ) backcrossed to C57BL/6 background. At 4 weeks after birth, the weight of various organs of the Lmna (-/-), Lmna (+/-) and Lmna (+) (/) (+) mice was measured. A panel of biochemical analyses consisting of 15 serological tests was examined. The results showed that Lmna deficient mice had significantly decreased body weight and increased the ratio of organ to body weight in most of tissues. Compared with Lmna (+) (/) (+) and Lmna (+/-) mice, Lmna (-/-) mice exhibited lower levels of ALP (alkaline phosphatase), Chol (cholesterol), CR (creatinine), GLU (glucose), HDL (high-density lipoprotein cholesterol) and higher levels of ALT (alanine aminotransferase) (p<0.05). Lmna (-/-) mice displayed higher AST (aspartate aminotransferase) values and lower LDL (lowdensity lipoprotein cholesterol), CK-MB (creatine kinase-MB) levels than Lmna (+) (/) (+) mice (p<0.05). There were no significant differences among the three groups of mice with respect to BUN (blood urea nitrogen), CK (creatine kinase), Cyc C (cystatin C), TP (total protein), TG (triacylglycerols) and UA (uric acid) levels (p>0.05). These changes of serological parameters may provide an experimental basis for the elucidation of Lmna gene functions.
authors: Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, China.
authors: J
authors: J
authors: Ruan
authors: Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, China.
authors: X G
authors: XG
authors: Liu
authors: Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, China.
authors: H L
authors: HL
authors: Zheng
authors: Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, China.
authors: J B
authors: JB
authors: Li
authors: Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, China.
authors: X D
authors: XD
authors: Xiong
authors: Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, China.
authors: C L
authors: CL
authors: Zhang
authors: Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, China.
authors: C Y
authors: CY
authors: Luo
authors: Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, China.
authors: Z J
authors: ZJ
authors: Zhou
authors: Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, China.
authors: Q
authors: Q
authors: Shi
authors: Department of Clinical Laboratory, Chongqing Medical University, Chongqing 400016, China.
authors: Y G
authors: YG
authors: Weng
keywords: Growth Delay; Lmna Deficient Mice; Serological Changes