title: Effect of Rougan Huaqian granules combined with human mesenchymal stem cell transplantation on liver fibrosis in cirrhosis rats.
abstract: OBJECTIVE
  To observe the effect of Rougan Huaqian granules combined with human mesenchymal stem cell (hMSC) transplantation on the liver fibrosis in carbon tetrachloride-induced cirrhosis rats.
  
  
  METHODS
  Sixty SD rats were randomly divided into five groups. The rats in control group received intraperitoneal injection of saline, while those in model control group, treatment group A, group B and group C received intraperitoneal injection of carbon tetrachloride oily solution to induce liver cirrhosis within 8 weeks. Then, the rats in the model control group, treatment group A, treatment group B, treatment group C received vein tail injection of saline, Rougan Huaqian granules, hMSC suspension and Rougan Huaqian granules combined with hMSC suspension.
  
  
  RESULTS
  The treatment groups had significantly different liver function (AST levels), liver fibrosis index (laminin and HA), hepatic sinusoidal wallsα-smooth muscle actin, IV collagen and laminin protein expression and I, III collagen from the model group (P<0.05). The transplanted cells showed human hepatocyte-like cells differentiation trend in the liver.
  
  
  CONCLUSIONS
  The Rougan Huaqian granules combined with hMSC transplantation can alleviate liver fibrosis in cirrhosis rats.
authors: Liver Disease Center, The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning 530023, China.
authors: Zhen-Chang
authors: ZC
authors: Wang
authors: Guangxi University of Traditional Chinese Medicine, Nanning 530007, China.
authors: Shan
authors: S
authors: Yang
authors: Liver Disease Center, The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning 530023, China.
authors: Jing-Jing
authors: JJ
authors: Huang
authors: Liver Disease Center, The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning 530023, China.
authors: Song-Lin
authors: SL
authors: Chen
authors: Guangxi University of Traditional Chinese Medicine, Nanning 530007, China.
authors: Quan-Qiang
authors: QQ
authors: Li
authors: Liver Disease Center, The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning 530023, China. Electronic address: 994liyuan@163.com.
authors: Yuan
authors: Y
authors: Li
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003094:Collagen / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D003094:Collagen; D053509:Matrix Metalloproteinase 13
keywords: Cirrhosis; Human cord blood mesenchymal stem cells; Rougan Huaqian granules; Transplantation; Treatment