title: [Effect of low-molecular-weight heparin combined with doxorubicin on hepatocellular cancer cell migration in vitro].
abstract: OBJECTIVE
  To investigate the anti-cancer effect of low-molecular-weight heparin (LMWH) combined with doxorubicin and explore the mechanism.
  
  
  METHODS
  Hepatocellular cancer HepG2 cells exposed to LMWH, doxorubicin, or both were evaluated for cell viability with MTT assay and for changes in their migration ability using wound healing assay and Transwell migration assay. The changes in cellular expressions of matrix metalloproteinase-9 (MMP-9) and MMP-2 mRNA and proteins were analyzed with quantitative real-time PCR (qRT-PCR) and Western blotting, and ELISA was used to determine heparanase (HPA) concentration in the cell culture medium.
  
  
  RESULTS
  HepG2 cells exhibited suppressed proliferation in response to LMWH and doxorubicin treatments. The combined treatment caused a significantly higher inhibition rate of cell migration than LMWH and doxorubicin alone. LMWH enhanced doxorubicin-induced down-regulation of MMP-9, MMP-2 and HPA in the cells.
  
  
  CONCLUSIONS
  LMWH can enhance the inhibitory effect of doxorubicin on the migration of HepG2 cells, the mechanism of which may involve the down-regulation of MMP-9, MMP-2 and HPA expressions.
authors: Department of Pharmacy, Bengbu Medical College, Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals, Bengbu 233030, China. E-mail: yangkehong2007@163.com.
authors: Kehong
authors: K
authors: Yang
authors: Linyan
authors: L
authors: Ma
authors: Xiu
authors: X
authors: Cheng
authors: Chao
authors: C
authors: Chen
authors: Mengxiao
authors: M
authors: Zhang
authors: Hao
authors: H
authors: Liu
authors: Zhiwen
authors: Z
authors: Jiang
mesh_terms: D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival; D015536:Down-Regulation; D004317:Doxorubicin / Q000494:pharmacology*; D005966:Glucuronidase / Q000737:chemistry; D056945:Hep G2 Cells; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D000099175:Heparanase
chemical_list: D004317:Doxorubicin; D005966:Glucuronidase; D006495:Heparin, Low-Molecular-Weight; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D012333:RNA, Messenger; D000099175:Heparanase; C522361:MMP2 protein, human; C579270:MMP9 protein, human