title: In-vivo imaging of psoriatic lesions with polarization multispectral dermoscopy and multiphoton microscopy.
abstract: Psoriasis is a skin autoimmune disease characterized by hyperkeratosis, hyperproliferation of the epidermis and dilatation of dermal papillary blood vessels. Healthy skin (5 volunteers) and psoriatic lesions (3 patients) were visualized in vivo, with high contrast and resolution, with a Polarization Multispectral Dermoscope and a Multiphoton Microscope. Psoriatic features were identified and quantified. The effective diameter of the superficial blood vessels was measured at 35.2 ± 7.2 μm and the elongated dermal papillae had an effective diameter of 64.2 ± 22.6 μm. The methodologies developed could be employed for quantitative diagnostic purposes and furthermore serve as a monitoring method of the effect of personalized treatments.
authors: European Laboratory for Non-Linear Spectroscopy (LENS) University of Florence, Sesto-Fiorentino, 50019, Italy.
authors: Dimitrios
authors: D
authors: Kapsokalyvas
authors: European Laboratory for Non-Linear Spectroscopy (LENS) University of Florence, Sesto-Fiorentino, 50019, Italy ; National Institute of Optics, National Research Council (INO-CNR), 50125, Florence, Italy.
authors: Riccardo
authors: R
authors: Cicchi
authors: Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, 50129, Florence, Italy.
authors: Nicola
authors: N
authors: Bruscino
authors: Light4tech Firenze srl, 50018, Scandicci, Italy.
authors: Domenico
authors: D
authors: Alfieri
authors: Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, 50129, Florence, Italy.
authors: Francesca
authors: F
authors: Prignano
authors: Department of Surgery and Translational Medicine, University of Florence, 50134, Florence, Italy.
authors: Daniela
authors: D
authors: Massi
authors: Department of Dermatology and Venereology, University Guglielmo Marconi, 00193, Rome, Italy.
authors: Torello
authors: T
authors: Lotti
authors: European Laboratory for Non-Linear Spectroscopy (LENS) University of Florence, Sesto-Fiorentino, 50019, Italy ; National Institute of Optics, National Research Council (INO-CNR), 50125, Florence, Italy ; Department of Physics, University of Florence, 50019, Sesto Fiorentino, Italy.
authors: Francesco S
authors: FS
authors: Pavone
keywords: (100.2980) Image enhancement; (170.1870) Dermatology; (170.6510) Spectroscopy, tissue diagnostics; (170.6900) Three-dimensional microscopy; (180.4315) Nonlinear microscopy