title: Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion.
abstract: OBJECTIVE
  To investigate the potential role of prostasin, as an invasion suppressor, in the process of trophoblast invasion in preeclampsia.
  
  
  METHODS
  This case-control study included 19 early-onset severe preeclampsia (⩽ 34 weeks), 20 late-onset severe preeclampsia (>34 weeks) and 20 normal term pregnant women. Immunohistochemistry was conducted to identify the cellular localization of prostasin, as well as the matrix metalloproteinase 2 (MMP2) and MMP9 in the placenta tissues. Enzyme-linked immunosorbent assay was performed to analyze the expression of these three proteins in placental homogenates. The effect of prostasin on the invasive and migratory ability of trophoblast cells was detected by transwell assays. We also examined the regulation of the prostasin antibody in the MMP2 and MMP9 secretion by HTR-8/SVneo cells via blocking the prostasin activity.
  
  
  RESULTS
  This study demonstrated that the prostasin, MMP2 and MMP9 were all expressed in the placental syncytiotrophoblasts. Increased expression of prostasin was detected in cases with early-onset severe preeclampsia compared with the late-onset and control groups (P < 0.05), whereas the expression patterns of MMP2 and MMP9 in placental homogenates were opposite to that of prostasin (P < 0.05). Recombinant prostasin inhibited the invasion and migration of trophoblast cells, whereas prostasin antibody enhanced the MMP2 and MMP9 secretion in a dose- and time-dependent manner.
  
  
  CONCLUSIONS
  These findings suggest that prostasin may suppress the invasion process in preeclampsia by attenuating MMP2 and MMP9 secretion.
authors: Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.
authors: Y
authors: Y
authors: Yang
authors: Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.
authors: J
authors: J
authors: Zhang
authors: Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.
authors: Y
authors: Y
authors: Gong
authors: Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.
authors: X
authors: X
authors: Liu
authors: Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.
authors: Y
authors: Y
authors: Bai
authors: 1] Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China [2] Sichuan University-The Chinese University of Hong Kong Joint Laboratory for Reproductive Medicine, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University, Chengdu, China.
authors: W
authors: W
authors: Xu
authors: Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.
authors: R
authors: R
authors: Zhou
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D010920:Placenta / Q000737:chemistry; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000503:physiopathology; D011247:Pregnancy; D012697:Serine Endopeptidases / Q000032:analysis / Q000502:physiology*; D014327:Trophoblasts / Q000502:physiology*; D000099271:Prostasin
chemical_list: D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D012697:Serine Endopeptidases; D000099271:Prostasin