title: Fluoroalcohols-induced modulation and amyloid formation in conalbumin.
abstract: In this study we are reporting the conformational modulation as well as the plausible mechanism of structural perturbation in conalbumin (CA). The overall structure of CA is altered by the change in dielectric constant upon addition of fluoroalcohols (TFE and HFIP). We studied the formation of aggregates in CA at pH 7.0 by multiple structural probes in the presence of TFE and HFIP. The protein aggregates maximally in 15% (v/v) TFE and 3% (v/v) HFIP. ANS, ThT binding and transmission electron microscopy data suggest that the aggregates induced by TFE and HFIP have amyloid-like properties. Higher concentrations of TFE and HFIP causes increase in helical propensity. Far-UV circular dichroism, intrinsic fluorescence, ANS and ThT fluorescence data suggests that formation of a partially structured intermediate state precedes the onset of the aggregation process.
authors: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
authors: Mohsin Vahid
authors: MV
authors: Khan
authors: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
authors: Gulam
authors: G
authors: Rabbani
authors: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
authors: Ejaz
authors: E
authors: Ahmad
authors: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India. Electronic address: rizwanhkhan@hotmail.com.
authors: Rizwan Hasan
authors: RH
authors: Khan
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D002942:Circular Dichroism; D003207:Conalbumin / Q000737:chemistry*; D008954:Models, Biological; D011485:Protein Binding; D017510:Protein Folding; D012997:Solvents / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000682:Amyloid; D012997:Solvents; D003207:Conalbumin
keywords: Amyloid fibrils; Circular dichroism; Conalbumin; Thioflavin T; Transmission electron microscopy