title: Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1.
abstract: The directed movement of cells is critical for numerous developmental and disease processes. A developmentally reiterated form of migration is radial intercalation; the process by which cells move in a direction orthogonal to the plane of the tissue from an inner layer to an outer layer. We use the radial intercalation of cells into the skin of Xenopus laevis embryos as a model to study directed cell migration within an epithelial tissue. We identify a novel function for both the microtubule-binding protein CLAMP and members of the microtubule-regulating Par complex during intercalation. Specifically, we show that Par3 and aPKC promote the apical positioning of centrioles, whereas CLAMP stabilizes microtubules along the axis of migration. We propose a model in which the Par complex defines the orientation of apical migration during intercalation and in which subcellular localization of CLAMP promotes the establishment of an axis of microtubule stability required for the active migration of cells into the outer epithelium.
authors: Department of Cell and Molecular Biology and Driskill Graduate Program in Life Sciences, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611.
authors: Michael E
authors: ME
authors: Werner
authors: Department of Cell and Molecular Biology and Driskill Graduate Program in Life Sciences, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 brian-mitchell@northwestern.edu.
authors: Jennifer W
authors: JW
authors: Mitchell
authors: Department of Cell and Molecular Biology and Driskill Graduate Program in Life Sciences, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611.
authors: William
authors: W
authors: Putzbach
authors: Department of Cell and Molecular Biology and Driskill Graduate Program in Life Sciences, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611.
authors: Elizabeth
authors: E
authors: Bacon
authors: Department of Cell and Molecular Biology and Driskill Graduate Program in Life Sciences, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611.
authors: Sun K
authors: SK
authors: Kim
authors: Department of Cell and Molecular Biology and Driskill Graduate Program in Life Sciences, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 brian-mitchell@northwestern.edu.
authors: Brian J
authors: BJ
authors: Mitchell
mesh_terms: D000818:Animals; D002465:Cell Movement; D016764:Cell Polarity; D002502:Centrioles / Q000378:metabolism; D000078404:Epidermal Cells; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism; D055550:Protein Stability; D021381:Protein Transport; D029867:Xenopus Proteins / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes; C000592111:Spef1 protein, Xenopus; D029867:Xenopus Proteins; C115015:PKC-3 protein; D011493:Protein Kinase C