title: A bimodal optoelectronic flow-through detector for phosphate determination.
abstract: A miniature flow-through detector useful for bimodal, photometric and fluorimetric, determination of phosphates has been developed. This optoelectronic device made of four light emitting diodes (LEDs) integrated in the form of 85 µL optical cell is easily applied in flow analysis manifolds. These LEDs play the roles of light source for photometric measurements, fluorescence inductors and detector of absorbance and fluorescence. For photometric mode of determinations a phosphomolybdenum blue method has been applied. The fluorimetric method of phosphate determination is based on quenching of rhodamine fluorescence by the heteropolyacid. The developed detector used in a simple three-channel flow injection analysis (FIA) system allows photometric or fluorimetric determination of phosphate in the wide range of concentration. The detection limits found for photometric and fluorimetric modes of FIA measurements are 5.5 mg L(-1) and 10.4 µg L(-1), respectively. The potential utility of the flow-through detector for the needs of food and clinical analysis has been demonstrated.
authors: University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
authors: Marta
authors: M
authors: Fiedoruk
authors: University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
authors: Elżbieta
authors: E
authors: Mieczkowska
authors: University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
authors: Robert
authors: R
authors: Koncki
authors: University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland. Electronic address: luktym@chem.uw.edu.pl.
authors: Lukasz
authors: L
authors: Tymecki
mesh_terms: D002253:Carbonated Beverages / Q000032:analysis; D015314:Dialysis Solutions / Q000032:analysis / Q000737:chemistry; D004581:Electronics / Q000295:instrumentation; D017022:Flow Injection Analysis / Q000379:methods*; D005470:Fluorometry / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007688:Kidneys, Artificial; D055095:Optics and Photonics / Q000295:instrumentation; D010710:Phosphates / Q000032:analysis*; D015203:Reproducibility of Results; D012235:Rhodamines / Q000737:chemistry
chemical_list: D015314:Dialysis Solutions; D010710:Phosphates; D012235:Rhodamines; C026188:rhodamine 6G; C029773:rhodamine B
keywords: Flow analysis; Fluorimetry; Optoelectronic detector; Phosphate; Photometry