title: Lectin from Agrocybe aegerita as a glycophenotype probe for evaluation of progression and survival in colorectal cancer.
abstract: BACKGROUND
  Agrocybe aegerita Lectin (AAL) has been identified to have high affinity for sulfated and α2-3- linked sialic acid glycoconjugates, especially the sulfated and sialyl TF (Thomsen-Friedenreich) disaccharide. This study was conducted to investigate the clinicopathological and prognostic value of AAL in identifying aberrant glycosylation in colorectal cancer (CRC).
  
  
  METHODS
  Glycoconjugate expression in 59 CRC tissues were detected using AAL-histochemistry. Clinicopathological associates of expression were analyzed with chi- square test or Fisher's exact test. Relationships between expression and the various clinicopathological parameters was estimated using Kaplan-Meier analysis and Cox regression models.
  
  
  RESULTS
  AAL specific glycoconjugate expression was significantly higher in tumor than corresponding normal tissues (66.1% and 46.1%, respectively, p=0.037), correlating with depth of invasion (p=0.015) and TNM stage (p=0.024). Patients with lower expression levels had a significantly higher survival rate than those with higher expression (p=0.046 by log rank test and p=0.047 by Breslow test for overall survival; p=0.054 by log rank test and P=0.038 by Breslow test for progress free survival). A marginally significant association was found between AAL specific glycoconjugate expression and overall survival by univariate Cox regression analysis (p=0.059).
  
  
  CONCLUSIONS
  Lower AAL specific glycoconjugate expression is a significant favorable prognostic factor for overall and progress free survival in CRC. This is the first report about the employment of AAL for histochemical analysis of cancer tissues. The binding characteristics of AAL means it has potential to become a powerful tool for the glycan investigation and clinical application.
authors: Department of Clinical Immunology, Guangdong Medical College, Dongguan, China E-mail : sunhui@whu.edu.cn, xingguichen@hotmail.com.
authors: Yi
authors: Y
authors: Liang
authors: Hua
authors: H
authors: Chen
authors: Han-Bin
authors: HB
authors: Zhang
authors: Yan-Xia
authors: YX
authors: Jin
authors: Hong-Qiang
authors: HQ
authors: Guo
authors: Xing-Gui
authors: XG
authors: Chen
authors: Hui
authors: H
authors: Sun
mesh_terms: D055362:Agrocybe / Q000378:metabolism*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000401:mortality* / Q000473:pathology; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D006031:Glycosylation; D006801:Humans; D053208:Kaplan-Meier Estimate; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D015996:Survival Rate
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D037102:Lectins; C016467:Thomsen-Friedenreich antigen