title: Enzymatic synthesis using glycoside phosphorylases.
abstract: Carbohydrate phosphorylases are readily accessible but under-explored catalysts for glycoside synthesis. Their use of accessible and relatively stable sugar phosphates as donor substrates underlies their potential. A wide range of these enzymes has been reported of late, displaying a range of preferences for sugar donors, acceptors and glycosidic linkages. This has allowed this class of enzymes to be used in the synthesis of diverse carbohydrate structures, including at the industrial scale. As more phosphorylase enzymes are discovered, access to further difficult to synthesise glycosides will be enabled. Herein we review reported phosphorylase enzymes and the glycoside products that they have been used to synthesise.
authors: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors: Ellis C
authors: EC
authors: O'Neill
authors: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: rob.field@jic.ac.uk.
authors: Robert A
authors: RA
authors: Field
mesh_terms: D000818:Animals; D060326:Chemistry Techniques, Synthetic / Q000379:methods*; D006027:Glycosides / Q000378:metabolism*; D006801:Humans; D006005:Phosphorylases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering
chemical_list: D006027:Glycosides; D006005:Phosphorylases
keywords: Glycosides; Phosphorylases; Synthesis