title: Directly decoupled diffusion-ordered NMR spectroscopy for the analysis of compound mixtures.
abstract: For the analysis of compound mixtures by NMR spectroscopy, it is important to assign the different peaks to the individual constituents. Diffusion-ordered spectroscopy (DOSY) is often used for the separation of signals based on their self-diffusion coefficient. However, this method often fails in the case of signal overlap, which is a particular problem for (1)H-detected DOSY spectra. Herein, an approach that allows the acquisition of homonuclear broadband-decoupled DOSY spectra without the introduction of an additional decoupling dimension, by instant decoupling during acquisition, is presented. It was demonstrated on a mixture of six alcohols, and the investigation of the binding of a dodecapeptide to membrane mimetics.
authors: Institute of Chemistry/Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz (Austria).
authors: Simon
authors: S
authors: Glanzer
authors: Klaus
authors: K
authors: Zangger
keywords: DOSY spectroscopy; NMR spectroscopy; homonuclear broadband decoupling; mixture analysis; pure shift