title: Changes in microbial diversity, methanogenesis and fermentation characteristics in the rumen in response to medicinal plant extracts.
abstract: This study evaluated the in vitro effect of medicinal plant extracts on ruminal methanogenesis, four different groups of methanogens and ruminal fermentation characteristics. A fistulated Holstein cow was used as a donor of rumen fluid. Licorice and mugwort extracts (Glycyrrhiza uralensis and Artemisia capillaris, 0.5% and 1% of total substrate DM, respectively), previously used as folk remedies, were added to an in vitro fermentation incubated with buffered-rumen fluid. Total gas production in Glycyrrhiza uralensis extract treatment was not significantly different between treatments (p<0.05) while total gas production in the Artemisia capillaris extract treatment was lower than that of the control. Artemisia capillaris extract and Glycyrrhiza uralensis extract reduced CH4 emission by 14% (p<0.05) and 8% (p<0.05), respectively. Ciliate-associated methanogens population decreased by 18% in the medicinal plant extracts treatments. Medicinal plant extracts also affected the order Methanobacteriales community. Methanobacteriales diversity decreased by 35% in the Glycyrrhiza uralensis extract treatment and 30% in the Artemisia capillaris extract treatment. The order Methanomicrobiales population decreased by 50% in the 0.5% of Glycyrrhiza uralensis extract treatment. These findings demonstrate that medicinal plant extracts have the potential to inhibit in vitro ruminal methanogenesis.
authors: Division of Applied Life Science, Graduate School of Gyeongsang National University (Institute of Agriculture and Life Science), Jinju, 660-701, Korea.
authors: Eun Tae
authors: ET
authors: Kim
authors: Division of Applied Life Science, Graduate School of Gyeongsang National University (Institute of Agriculture and Life Science), Jinju, 660-701, Korea.
authors: Yea Hwang
authors: YH
authors: Moon
authors: Division of Applied Life Science, Graduate School of Gyeongsang National University (Institute of Agriculture and Life Science), Jinju, 660-701, Korea.
authors: Kwan-Sik
authors: KS
authors: Min
authors: Division of Applied Life Science, Graduate School of Gyeongsang National University (Institute of Agriculture and Life Science), Jinju, 660-701, Korea.
authors: Chang-Hyun
authors: CH
authors: Kim
authors: Division of Applied Life Science, Graduate School of Gyeongsang National University (Institute of Agriculture and Life Science), Jinju, 660-701, Korea.
authors: Sam Churl
authors: SC
authors: Kim
authors: Division of Applied Life Science, Graduate School of Gyeongsang National University (Institute of Agriculture and Life Science), Jinju, 660-701, Korea.
authors: Seung Kyu
authors: SK
authors: Ahn
authors: Division of Applied Life Science, Graduate School of Gyeongsang National University (Institute of Agriculture and Life Science), Jinju, 660-701, Korea.
authors: Sung Sill
authors: SS
authors: Lee
keywords: Medicinal Plant Extracts; Methane Emission; Real-time PCR; Relative Quantification; Ruminal Methanogenesis