title: Bone mechanobiology, gravity and tissue engineering: effects and insights.
abstract: Bone homeostasis strongly depends on fine tuned mechanosensitive regulation signals from environmental forces into biochemical responses. Similar to the ageing process, during spaceflights an altered mechanotransduction occurs as a result of the effects of bone unloading, eventually leading to loss of functional tissue. Although spaceflights represent the best environment to investigate near-zero gravity effects, there are major limitations for setting up experimental analysis. A more feasible approach to analyse the effects of reduced mechanostimulation on the bone is represented by the 'simulated microgravity' experiments based on: (1) in vitro studies, involving cell cultures studies and the use of bioreactors with tissue engineering approaches; (2) in vivo studies, based on animal models; and (3) direct analysis on human beings, as in the case of the bed rest tests. At present, advanced tissue engineering methods allow investigators to recreate bone microenvironment in vitro for mechanobiology studies. This group and others have generated tissue 'organoids' to mimic in vitro the in vivo bone environment and to study the alteration cells can go through when subjected to unloading. Understanding the molecular mechanisms underlying the bone tissue response to mechanostimuli will help developing new strategies to prevent loss of tissue caused by altered mechanotransduction, as well as identifying new approaches for the treatment of diseases via drug testing. This review focuses on the effects of reduced gravity on bone mechanobiology by providing the up-to-date and state of the art on the available data by drawing a parallel with the suitable tissue engineering systems.
authors: University of Genova, Department of Experimental Medicine, Genova, Italy.
authors: Alessandra
authors: A
authors: Ruggiu
authors: University of Genova, Department of Experimental Medicine & IRCCS AOU San Martino-IST, National Institute for Cancer Research, Genova, Italy.
authors: Ranieri
authors: R
authors: Cancedda
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D006801:Humans; D040542:Mechanotransduction, Cellular; D023822:Tissue Engineering / Q000379:methods*; D014893:Weightlessness
keywords: bone; gravity; human; mechanobiology; microgravity simulation; rodents; spaceflight; tissue engineering