title: IRS-2 G1057D polymorphism in Turkish patients with colorectal cancer.
abstract: BACKGROUND
  Gene polymorphisms have a broad range of analysis, but are of particular use in molecular medicine due to their potential in revealing the genetic tendency in diseases such as cancer, heart attack etc. These studies basically depend on mutations that can be detected by proper techniques. The genes coding the insulin receptor substrate (IRS) proteins are among the most widely analysed polymorphisms in various cancer types, in which a G1057D mutation is seen.
  
  
  OBJECTIVE
  To determine the risk of colon cancer by analysing the IRS-2 gene polymorphism in Turkish patients.
  
  
  METHODS
  A total of 161 newly diagnosed colorectal cancer patients were analysed and compared to 197 unrelated healthy controls. A polymerase chain reaction-based restriction fragment length polymorphism method was carried out.
  
  
  RESULTS
  No differences were observed between the patient and control groups for both allele and genotype frequencies of the IRS-2 G1057D gene.
  
  
  CONCLUSIONS
  Our results demonstrated that IRS-2 G1057D polymorphism is not associated with colorectal cancer in the Turkish population. This research is a preliminary and original study in Turkish patients with colorectal cancer. It also provides population-level genetic data on IRS-2 in the Turkish population. Further studies should be performed on larger number of patients and controls for more reliable results about the genetic tendency in colorectal cancer in Turkey. The study is a collaborative work of different universities and scientists.
authors: Institute of Forensic Sciences, Istanbul University, Cerrahpasa, Istanbul, Turkey.
authors: Emel Hulya
authors: EH
authors: Yukseloglu
authors: Department of Medical Biology and Genetics, Medical Faculty, Bulent Ecevit University, Zonguldak, Turkey.
authors: Sevim Karakas
authors: SK
authors: Celik
authors: Department of Biology, Faculty of Art and Science, Adiyaman University, Adiyaman, Turkey.
authors: Meral Urhan
authors: MU
authors: Kucuk
authors: Department of Biochemistry, Medical Faculty, Cukurova University, Adana, Turkey.
authors: Erdinc
authors: E
authors: Yalin
authors: Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Yeni Yuzyil University, Istanbul, Turkey.
authors: S Sebnem
authors: SS
authors: Ozkal
authors: General Surgery, Toros Government Hospital, Mersin, Turkey.
authors: Cengiz
authors: C
authors: Ates
authors: Department of Pharmaceutical Biotechnology, Pharmacy Faculty, Yuzuncu Yil University, Van, Turkey.
authors: Mehmet
authors: M
authors: Berkoz
authors: Department of Biochemistry, Pharmacy Faculty, Mersin University, Mersin, Turkey.
authors: Serap
authors: S
authors: Yalin
authors: Department of Medical Biology and Genetics, Medical Faculty, Mersin University, Mersin, Turkey.
authors: Nurcan Aras
authors: NA
authors: Ates
keywords: IRS-2; Turkey; colon cancer; polymorphism