title: The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription.
abstract: Bud27, the yeast orthologue of human URI/RMP, is a member of the prefoldin-like family of ATP-independent molecular chaperones. It has recently been shown to mediate the assembly of the three RNA polymerases in an Rpb5-dependent manner. In this work, we present evidence of Bud27 modulating RNA pol II transcription elongation. We show that Bud27 associates with RNA pol II phosphorylated forms (CTD-Ser5P and CTD-Ser2P), and that its absence affects RNA pol II occupancy of transcribed genes. We also reveal that Bud27 associates in vivo with the Sth1 component of the chromatin remodeling complex RSC and mediates its association with RNA pol II. Our data suggest that Bud27, in addition of contributing to Rpb5 folding within the RNA polymerases, also participates in the correct assembly of other chromatin-associated protein complexes, such as RSC, thereby modulating their activity.
authors: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071, Jaén, Spain.
authors: María Carmen
authors: MC
authors: Mirón-García
authors: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071, Jaén, Spain.
authors: Ana Isabel
authors: AI
authors: Garrido-Godino
authors: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071, Jaén, Spain.
authors: Verónica
authors: V
authors: Martínez-Fernández
authors: Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, E-41013 Sevilla, Spain Departamento de Genética, Universidad de Sevilla, E41012 Sevilla, Spain.
authors: Antonio
authors: A
authors: Fernández-Pevida
authors: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071, Jaén, Spain.
authors: Abel
authors: A
authors: Cuevas-Bermúdez
authors: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071, Jaén, Spain.
authors: Manuel
authors: M
authors: Martín-Expósito
authors: Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, E-41013 Sevilla, Spain Departamento de Genética, Universidad de Sevilla, E41012 Sevilla, Spain.
authors: Sebastián
authors: S
authors: Chávez
authors: Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, E-41013 Sevilla, Spain Departamento de Genética, Universidad de Sevilla, E41012 Sevilla, Spain.
authors: Jesús
authors: J
authors: de la Cruz
authors: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071, Jaén, Spain fngomez@ujaen.es.
authors: Francisco
authors: F
authors: Navarro
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000235:genetics / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D061805:Transcription Elongation, Genetic
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D009687:Nuclear Proteins; D010448:Peptide Initiation Factors; D029701:Saccharomyces cerevisiae Proteins; C541141:Uri1 protein, S cerevisiae; D012319:RNA Polymerase II; C511736:RPB1 protein, S cerevisiae; C077392:STH1 protein, S cerevisiae