title: Ultrasonication aided in-situ transesterification of microbial lipids to biodiesel.
abstract: In-situ transesterification of microbial lipid to biodiesel has been paid substantial attention due to the fact that the lipid extraction and transesterification can be conducted in one-stage process. To improve the feasibility of in-situ transesterification, ultrasonication was employed to reduce methanol requirement and reaction time. The results showed that the use of ultrasonication could achieve high conversion of lipid to FAMEs (92.1% w lipid conversion/w total lipids) with methanol to lipid molar ratio 60:1 and NaOH addition 1% w/w lipid in 20 min, while methanol to lipid molar ratio 360:1, NaOH addition 1% w/w lipid, and reaction time 12h was required to obtain similar yield in in-situ transesterification without ultrasonication. The compositions of FAMEs obtained in case of ultrasonication aided in-situ transesterification were similar as that of two-stage extraction followed by transesterification processes.
authors: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors: Xiaolei
authors: X
authors: Zhang
authors: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors: Song
authors: S
authors: Yan
authors: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada. Electronic address: rd.tyagi@ete.inrs.ca.
authors: Rajeshwar Dayal
authors: RD
authors: Tyagi
authors: Department of Civil Engineering, University of Nebraska-Lincoln, N104 SEC, PO Box 886105, Lincoln, NE 68588-6105, USA.
authors: Rao Y
authors: RY
authors: Surampalli
authors: INRS Eau, Terre et Environnement, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors: Jose R
authors: JR
authors: Valéro
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D004951:Esterification; D004952:Esters / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D000432:Methanol / Q000378:metabolism; D013010:Sonication / Q000379:methods*; D013997:Time Factors; D014250:Trichosporon / Q000378:metabolism*; D014465:Ultrasonics / Q000379:methods*
chemical_list: D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D008055:Lipids; D000432:Methanol
keywords: Biodiesel; In-situ transesterification; Microbial lipid; Ultrasonication