title: Amelioration of colitis in mouse model by exploring antioxidative potentials of an indigenous probiotic strain of Lactobacillus fermentum Lf1.
abstract: Based on the preliminary screening of eight indigenous putative probiotic Lactobacilli, Lactobacillus fermentum Lf1 was selected for assessing its antioxidative efficacy in DSS colitis mouse model based on its ability to enhance the expression of "Nrf2" by 6.43-fold and malondialdehyde (MDA) inhibition by 78.1 ± 0.24% in HT-29 cells under H2O2 stress. The Disease Activity Index and histological scores of Lf1-treated mice were lower than the control group. However, expression of "Nrf2" was not observed in Lf1-treated mice. A significant increase in the expression of antioxidative enzymes such as SOD2 and TrxR-1 was recorded in both of the groups. The expression of SOD2 was significantly downregulated in colitis-induced mice by -100.00-fold relative to control group, and the downregulation was considerably reduced to -37.04-fold in colitis Lf1 treatment group. Almost, a similar trend was recorded in case of "thioredoxin" expression, though "CAT" was refractile to expression. The Lf1-treated group had decreased malondialdehyde level as compared to colitis control (37.92 ± 6.31 versus 91.13 ± 5.76 μM/g). These results point towards Lf1-induced activation of the antioxidant enzyme system in the mouse model and its prospects to be explored as a new strategy for IBD management.
authors: Molecular Biology Unit, Department of Dairy Microbiology, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Ritu
authors: R
authors: Chauhan
authors: Molecular Biology Unit, Department of Dairy Microbiology, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Aparna Sudhakaran
authors: AS
authors: Vasanthakumari
authors: Molecular Biology Unit, Department of Dairy Microbiology, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Harsh
authors: H
authors: Panwar
authors: Molecular Biology Unit, Department of Dairy Microbiology, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Rashmi H
authors: RH
authors: Mallapa
authors: Department of Food Engineering and Technology, Tezpur University, Napaam, Assam 784028, India.
authors: Raj Kumar
authors: 0000-0002-1186-6999
authors: RK
authors: Duary
authors: Molecular Biology Unit, Department of Dairy Microbiology, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Virender Kumar
authors: 0000-0001-9186-5138
authors: VK
authors: Batish
authors: Molecular Biology Unit, Department of Dairy Microbiology, National Dairy Research Institute, Karnal, Haryana 132001, India.
authors: Sunita
authors: 0000-0003-0088-2245
authors: S
authors: Grover
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D052197:Limosilactobacillus fermentum; D008297:Male; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D019936:Probiotics / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D000099156:Superoxide Dismutase 2
chemical_list: D000975:Antioxidants; D016264:Dextran Sulfate; D006861:Hydrogen Peroxide; D051267:NF-E2-Related Factor 2; D016877:Oxidants; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2; C495636:Nfe2l2 protein, mouse