title: Hepatoprotective effects of loach (Misgurnus anguillicaudatus) lyophilized powder on dimethylnitrosamine-induced liver fibrosis in rats.
abstract: This study investigates the hepatoprotective effects and the potential therapeutic mechanisms of loach (Misgurnus anguillicaudatus) lyophilized powder (MLP) on dimethylnitrosamine (DMN) induced liver fibrosis in rats. After treatment with MLP (50, 100, 200 mg/kg), alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (Alb), total protein (TP) and hydroxyproline (Hyp) levels were detected, to assess the destruction of hepatocytes and the extent of liver fibrosis. Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), hyaluronic acid (HA), Laminin (LN), procollagen type-III (PC-III), collagen type-IV (C-IV), and transforming growth factor-β1(TGF-β1) contents in serum were all tested using ELISA kits. Alpha-smooth muscle actin (α-SMA) and the tissue inhibitor of metalloproteinase-1 (TIMP-1) protein contents and distribution were evaluated using western blot and immunohistochemical analysis. MLP significantly decreased the serum concentrations of ALT, AST, Hyp, HA, LN, PC-III, C-IV, MMP-2, TIMP-1, α-SMA and TGF-β1, while increasing the contents of Alb and MMP-9. No significant changes on TP serum concentrations were observed. These results suggest that MLP has anti-hepatic fibrosis effects and its mechanism may be associated with the attenuation of extracellular matrix (ECM) synthesis, the acceleration of ECM degradation, inhibition of hepatic stellate cells (HSCs) activation and TGF-β1expression.
authors: College of Pharmaceutical Sciences, Soochow University, 199 Ren'ai road, Suzhou, 215123, People's Republic of China.
authors: Qian
authors: Q
authors: Zhang
authors: College of Pharmaceutical Sciences, Soochow University, 199 Ren'ai road, Suzhou, 215123, People's Republic of China.
authors: Meng-Meng
authors: MM
authors: Shang
authors: School of Biology & Basic Medical Sciences, Soochow University, Suzhou, People's Republic of China.
authors: Qu-Fei
authors: QF
authors: Ling
authors: College of Pharmaceutical Sciences, Soochow University, 199 Ren'ai road, Suzhou, 215123, People's Republic of China.
authors: Xiao-Ping
authors: XP
authors: Wu
authors: College of Pharmaceutical Sciences, Soochow University, 199 Ren'ai road, Suzhou, 215123, People's Republic of China. liuchunyu@suda.edu.cn.
authors: Chun-Yu
authors: CY
authors: Liu
mesh_terms: D000818:Animals; D003531:Cypriniformes / Q000378:metabolism*; D004128:Dimethylnitrosamine; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D055166:Hepatic Stellate Cells / Q000187:drug effects / Q000473:pathology; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000517:prevention & control*; D008297:Male; D011208:Powders / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D011208:Powders; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D004128:Dimethylnitrosamine
keywords: Extracellular matrix; Hepatic stellate cells; Liver fibrosis; Loach (Misgurnus anguillicaudatus) lyophilized powder; Mechanism of anti-hepatic fibrosis; Rats