title: Knockdown of CUG-binding protein 1 induces apoptosis of human laryngeal cancer cells.
abstract: To investigate the role of CUG-binding protein 1 (CUGBP1) in human laryngeal cancer, we employed lentivirus-mediated short hairpin RNA (shRNA) to knockdown CUGBP1 expression in Hep-2 cells. Depletion of CUGBP1 remarkably inhibited the proliferation of Hep-2 cells. CUGBP1 knockdown induced cell cycle arrest in S phase, especially in the sub-G1 phase, representing apoptotic cells. Knockdown of CUGBP1 in Hep-2 cells markedly increased the expression of LIP and cleavage of PARP, which could contribute to apoptosis. Thus CUGBP1 has a critical role in modulating cell growth and apoptosis, and serves as a potential therapeutic target in laryngeal cancer.
authors: Department of Otolaryngology, Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, 100730, China; Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, 361003, China.
authors: Yi
authors: Y
authors: Zhou
authors: Hongzhi
authors: H
authors: Ma
authors: Jugao
authors: J
authors: Fang
authors: Meng
authors: M
authors: Lian
authors: Ling
authors: L
authors: Feng
authors: Ru
authors: R
authors: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D000067879:CELF1 Protein; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D055785:Gene Knockdown Techniques / Q000379:methods; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics*
chemical_list: D000067879:CELF1 Protein; C102843:CELF1 protein, human; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins
keywords: CUG-binding protein 1; growth; laryngeal cancer; short hairpin RNA