title: Imaging without fluorescence: nonlinear optical microscopy for quantitative cellular imaging.
abstract: Quantitative single-cell analysis enables the characterization of cellular systems with a level of detail that cannot be achieved with ensemble measurement. In this Feature we explore quantitative cellular imaging applications with nonlinear microscopy techniques. We first offer an introductory tutorial on nonlinear optical processes and then survey a range of techniques that have proven to be useful for quantitative live cell imaging without fluorescent labels.
authors: College of Engineering and Biodynamic Optical Imaging Center (BIOPIC), Peking University , Beijing, China , 100871.
authors: Aaron M
authors: AM
authors: Streets
authors: Ang
authors: A
authors: Li
authors: Tao
authors: T
authors: Chen
authors: Yanyi
authors: Y
authors: Huang
mesh_terms: D000818:Animals; D003585:Cell Biology / Q000295:instrumentation*; D045744:Cell Line, Tumor; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000737:chemistry; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008853:Microscopy; D059010:Single-Cell Analysis; D013059:Spectrum Analysis, Raman; D015027:Zebrafish
chemical_list: C031086:5-ethynyl-2'-deoxyuridine; D003857:Deoxyuridine