title: Increased oxidative damage associated with unfavorable cytogenetic subgroups in chronic lymphocytic leukemia.
abstract: Oxidative stress contributes to genomic instability in chronic lymphocytic leukemia (CLL), but its relationship with the acquisition of specific chromosomal abnormalities is unknown. We recruited 55 untreated CLL patients and assessed 8-oxo-2'-deoxyguanosine (8-oxo-dG), glutathione, and malondialdehyde (MDA) levels, and we compared them among the cytogenetic subgroups established using fluorescence in situ hybridization (FISH). Significant increases in 8-oxo-dG and/or MDA were observed in patients with unfavorable cytogenetic aberrations (17p and 11q deletions) compared to the 13q deletion group. TP53 deletion patients exhibited a diminished DNA repair efficiency. Finally, cases with normal FISH also showed enhanced 8-oxo-dG, which could result in adverse outcomes.
authors: Service of Hematology, CDB-University General Hospital of Valencia, Avenida Tres Cruces 2, 46014 Valencia, Spain.
authors: Rosa
authors: 0000-0002-8205-6192
authors: R
authors: Collado
authors: Department of Medicine, Faculty of Medicine, University of Valencia, Avenida Blasco Ibáñez 13, 46010 Valencia, Spain.
authors: David
authors: 0000-0003-3766-1606
authors: D
authors: Ivars
authors: Department of Medicine, Faculty of Medicine, University of Valencia, Avenida Blasco Ibáñez 13, 46010 Valencia, Spain.
authors: Isabel
authors: 0000-0002-4503-3303
authors: I
authors: Oliver
authors: CIBERobn, Biomedical Network Research Centre in Physiopathology of Obesity and Nutrition, Choupana s/n, 15706 Santiago de Compostela, Spain.
authors: Carmen
authors: C
authors: Tormos
authors: Service of Hematology, CDB-University General Hospital of Valencia, Avenida Tres Cruces 2, 46014 Valencia, Spain.
authors: Mercedes
authors: 0000-0002-1510-529X
authors: M
authors: Egea
authors: Service of Hematology, CDB-University General Hospital of Valencia, Avenida Tres Cruces 2, 46014 Valencia, Spain.
authors: Amparo
authors: 0000-0003-3390-9926
authors: A
authors: Miguel
authors: CIBERobn, Biomedical Network Research Centre in Physiopathology of Obesity and Nutrition, Choupana s/n, 15706 Santiago de Compostela, Spain ; Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Valencia, Avenida Blasco Ibáñez 13, 46010 Valencia, Spain ; Service of Clinical Analyses, CDB-University General Hospital of Valencia, Avenida Tres Cruces 2, 46014 Valencia, Spain.
authors: Guillermo T
authors: GT
authors: Sáez
authors: Service of Hematology, CDB-University General Hospital of Valencia, Avenida Tres Cruces 2, 46014 Valencia, Spain ; Department of Medicine, Faculty of Medicine, University of Valencia, Avenida Blasco Ibáñez 13, 46010 Valencia, Spain.
authors: Félix
authors: F
authors: Carbonell
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D015331:Cohort Studies; D004249:DNA Damage; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry; D005260:Female; D017353:Gene Deletion; D005978:Glutathione / Q000737:chemistry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D015227:Lipid Peroxidation; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000737:chemistry; D008875:Middle Aged; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
chemical_list: D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D005978:Glutathione