title: Lack of evidence for human infection with Xenotropic murine leukemia virus-related virus in the Brazilian Amazon basin.
abstract: BACKGROUND
  This study confirmed the absence of natural infection with Xenotropic murine leukemia virus-related virus (XMRV) or XMRV-related disease in human populations of the Brazilian Amazon basin. We demonstrated that 803 individuals of both sexes, who were residents of Belem in the Brazilian State of Pará, were not infected with XMRV.
  
  
  METHODS
  Individuals were divided into 4 subgroups: healthy individuals, individuals infected with human immunodeficiency virus, type 1 (HIV-1), individuals infected with human T-lymphotrophic virus, types 1 or 2 (HTLV-1/2), and individuals with prostate cancer. XMRV infection was investigated by nested PCR to detect the viral gag gene and by quantitative PCR to detect pol.
  
  
  RESULTS
  There was no amplification of either gag or pol segments from XRMV in any of the samples examined.
  
  
  CONCLUSIONS
  This study supports the conclusions of the studies that eventually led to the retraction of the original study reporting the association between XMRV and human diseases.
authors: Laboratório de Virologia, Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brazil.
authors: Samara Tatielle Monteiro
authors: ST
authors: Gomes
authors: Laboratório de Virologia, Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brazil.
authors: Luciana
authors: L
authors: Imbiriba
authors: Laboratório de Citogenética Humana, Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brazil.
authors: Rommel Rodriguéz
authors: RR
authors: Burbano
authors: Laboratório de Polimorfismo de DNA, Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brazil.
authors: Artur Luiz da Costa
authors: AL
authors: Silva
authors: Laboratório de Virologia, Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brazil.
authors: Rosimar Neris Martins
authors: RN
authors: Feitosa
authors: Laboratório de Virologia, Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brazil.
authors: Izaura Maria Vieira
authors: IM
authors: Cayres-Vallinoto
authors: Laboratório de Virologia, Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brazil.
authors: Marluísa de Oliveira Guimarães
authors: Mde O
authors: Ishak
authors: Laboratório de Virologia, Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brazil.
authors: Ricardo
authors: R
authors: Ishak
authors: Laboratório de Virologia, Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brazil.
authors: Antonio Carlos Rosário
authors: AC
authors: Vallinoto
mesh_terms: D000328:Adult; D001938:Brazil; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000821:virology*; D015490:HTLV-I Infections / Q000821:virology*; D015491:HTLV-II Infections / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000821:virology*; D012192:Retroviridae Infections / Q000150:complications*; D058008:Xenotropic murine leukemia virus-related virus / Q000235:genetics*
chemical_list: D004279:DNA, Viral