title: Gel filtration chromatography as a method for removing bacterial endotoxin from antibody preparations.
abstract: The removal of bacterial endotoxins from biological samples is critical to avoid the potentially fatal pyrogenic response possible when introduced to mammalian systems. Endotoxins have a variety of specific characteristics that can be exploited to target their isolation and subsequent removal, but one that has not been extensively characterized is their difference in size from that of monoclonal antibodies. Here, we present a study which utilizes gel filtration chromatography as a method for endotoxin removal from both aggregated and nonaggregated antibody preparations, outlining a mechanistically simple method for removal of this impurity.
authors: Novartis Institutes for Biomedical Research, Protein Production and Antibodies, Cambridge, MA.
authors: Anne Serdakowski
authors: AS
authors: London
authors: Kasey
authors: K
authors: Mackay
authors: Michael
authors: M
authors: Lihon
authors: Yaqin
authors: Y
authors: He
authors: Busola Ruth
authors: BR
authors: Alabi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000302:isolation & purification; D016466:CHO Cells; D002850:Chromatography, Gel / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D004731:Endotoxins / Q000302:isolation & purification*; D008823:Micelles
chemical_list: D000911:Antibodies, Monoclonal; D004731:Endotoxins; D008823:Micelles
keywords: endotoxin; protein purification