title: Multicolor analysis of cell surface marker of human leukemia cell lines using flow cytometry.
abstract: BACKGROUND
  Leukemia cell lines are utilized as tools for molecular analysis. Their implementation in therapy will require standards for quality control, including appropriate selection criteria for functional analysis and efficacy determination.
  
  
  METHODS
  Characteristics of six human leukemia cell lines -Kasumi-1, NB-4, MOLM-13, MV-4-11, K562, and Jurkat cells-were investigated using multiple color analysis of surface antigen expression and comparative analysis of gene expression.
  
  
  RESULTS
  Differentiation states of Kasumi-1 and MOLM-13 cells are colony-forming units-granulocyte/macrophage equivalent cells to myeloblasts with comparatively high Growth factor independent-1(GFI1) and Transcription factor PU.1 (PU.1) expression, respectively. NB4 and MV-4-11 express high levels of CCAAT/enhancer-binding protein-alpha (CEBPα) and differentiate from myeloblasts to pro-monocytes and myeloblasts, respectively. K562 cells are colony-forming units-erythroid equivalent cells to erythroblasts, with the highest expression of GATA-binding factor 2 (GATA2), GATA1 and Friend of gata-1 (FOG1). Jurkat cells are pro-T to mature T-cells with the highest Neurogenic locus notch-1 homolog protein 1 (NOTCH1) expression.
  
  
  CONCLUSIONS
  Our study gives a useful guideline of standards for appropriate usage of leukemia cell lines for examining novel targets in vitro.
authors: Department of Research and Development of Next-Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan yokotomo@hsc.med.kyushu-u.ac.jp ds-mons@yb3.so-net.ne.jp.
authors: Tomoko
authors: T
authors: Inoue
authors: Department of Research and Development of Next-Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors: Anthony
authors: A
authors: Swain
authors: Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan.
authors: Yoichi
authors: Y
authors: Nakanishi
authors: Department of Research and Development of Next-Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan yokotomo@hsc.med.kyushu-u.ac.jp ds-mons@yb3.so-net.ne.jp.
authors: Daisuke
authors: D
authors: Sugiyama
mesh_terms: D000954:Antigens, Surface / Q000032:analysis*; D045744:Cell Line, Tumor; D005434:Flow Cytometry / Q000379:methods*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007938:Leukemia / Q000276:immunology* / Q000473:pathology; D014157:Transcription Factors / Q000032:analysis
chemical_list: D000954:Antigens, Surface; D006684:HLA-DR Antigens; D014157:Transcription Factors
keywords: Leukemia cell line; cell surface marker; flow cytometry