title: Expression and immunohistochemical localization of leptin receptor in human periapical granuloma.
abstract: OBJECTIVE
  To investigate the expression and immunohistochemical localization of leptin receptor (LEPR) in human periapical granulomas.
  
  
  METHODS
  Periapical inflammatory lesions were obtained from extracted human teeth and teeth which underwent periapical surgery. After their histopathological categorization as periapical granulomas (n = 20), they were examined by immunohistochemistry using human LEPR monoclonal antibodies. LEPR mRNA expression was also determined by quantitative real-time PCR (qRT-PCR), and the amount of LEPR protein was analysed by immunoblot.
  
  
  RESULTS
  All granuloma samples expressed LEPR. Amongst inflammatory cells, only macrophages showed expression of LEPR. Western blot analysis revealed the presence in the samples of a protein with apparent molecular weight of ~120 kDa, corresponding to the estimated molecular weight of LEPR. The qRT-PCR analysis demonstrated the expression of LEPR mRNA, corresponding the size of the amplified fragment (338 bp), assessed by agarose gel electrophoresis, to that of LEPR mRNA.
  
  
  CONCLUSIONS
  Human periapical granulomas express LEPR. In periapical granulomas, only macrophages showed expression of LEPR. This finding suggests that leptin can play a role in inflammatory and immune periapical responses.
authors: Department of Stomatology (Endodontics Section), School of Dentistry, Virgen Macarena University Hospital, University of Sevilla, Sevilla, Spain.
authors: J
authors: J
authors: Martín-González
authors: Department of Medical Biochemistry and Molecular Biology, Virgen Macarena University Hospital, University of Sevilla, Sevilla, Spain.
authors: A
authors: A
authors: Carmona-Fernández
authors: Department of Medical Biochemistry and Molecular Biology, Virgen Macarena University Hospital, University of Sevilla, Sevilla, Spain.
authors: A
authors: A
authors: Pérez-Pérez
authors: Department of Medical Biochemistry and Molecular Biology, Virgen Macarena University Hospital, University of Sevilla, Sevilla, Spain.
authors: F
authors: F
authors: Sánchez-Jiménez
authors: Department of Medical Biochemistry and Molecular Biology, Virgen Macarena University Hospital, University of Sevilla, Sevilla, Spain.
authors: V
authors: V
authors: Sánchez-Margalet
authors: Department of Stomatology (Endodontics Section), School of Dentistry, Virgen Macarena University Hospital, University of Sevilla, Sevilla, Spain.
authors: J J
authors: JJ
authors: Segura-Egea
mesh_terms: D000368:Aged; D015153:Blotting, Western; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010484:Periapical Granuloma / Q000378:metabolism* / Q000601:surgery*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D054411:Receptors, Leptin / Q000378:metabolism*
chemical_list: C515448:LEPR protein, human; D012333:RNA, Messenger; D054411:Receptors, Leptin
keywords: apical granuloma; immune system; immunohistochemistry; leptin; leptin receptor; periapical inflammatory response