title: Systematic comparison of bacterial feeding strains for increased yield of Caenorhabditis elegans males by RNA interference-induced non-disjunction.
abstract: Rare Caenorhabditis elegans males arise when sex chromosome non-disjunction occurs during meiosis in self-fertilizing hermaphrodites. Non-disjunction is a relatively rare event, and males are typically observed at a frequency of less than one in five hundred wild-type animals. Males are required for genetic crosses and phenotypic analysis, yet current methods to generate large numbers of males can be cumbersome. Here, we identify RNAi reagents (dsRNA-expressing bacteria) with improved effectiveness for eliciting males. Specifically, we used RNAi to systematically reduce the expression of over two hundred genes with meiotic chromosome segregation functions, and we identified a set of RNAi reagents that robustly and reproducibly elicited male progeny.
authors: Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA.
authors: Lisa
authors: L
authors: Timmons
authors: Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA.
authors: Hayley
authors: H
authors: Luna
authors: Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA.
authors: Jordan
authors: J
authors: Martinez
authors: Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA.
authors: Zachary
authors: Z
authors: Moore
authors: Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA.
authors: Vaishnavi
authors: V
authors: Nagarajan
authors: Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA.
authors: Juliana Metichecchia
authors: JM
authors: Kemege
authors: Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA.
authors: Nadeem
authors: N
authors: Asad
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D020090:Chromosome Segregation / Q000235:genetics; D046508:Culture Techniques; D004032:Diet; D005260:Female; D018014:Gene Transfer Techniques; D008297:Male; D009630:Nondisjunction, Genetic; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D019849:Sex Determination Processes / Q000235:genetics
chemical_list: D029742:Caenorhabditis elegans Proteins
keywords: Caenorhabditis elegans males; Him phenotype; Kinesin-like protein; Non-disjunction; RNAi; dsRNA