title: Screening and identification of multiple components in Tanreqing injection using RP-HPLC combined with DAD and ESI-TOF/MS.
abstract: A liquid chromatography coupled with diode array detector (DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) method was developed for the screening and identification of the multiple components in Tanreqing injection, a well-known Chinese medicine injection in China. By combining the DAD spectrum and the accurate mass measurement of ESI-TOF/MS, twelve components in Tanreqing injection were identified. This study contributes to clarifying the nature of Tanreqing injection, and provides an effective and reliable process for the comprehensive and systematic characterization of complex traditional Chinese medicine preparations.
authors: Shanghai Kaibao Pharmaceutical Co. Ltd., Shanghai 201401, China.
authors: Shao-Yong
authors: SY
authors: Liu
authors: Shanghai Kaibao Pharmaceutical Co. Ltd., Shanghai 201401, China.
authors: Dong-Sheng
authors: DS
authors: Xue
authors: Shanghai Kaibao Pharmaceutical Co. Ltd., Shanghai 201401, China.
authors: Jian-Chao
authors: JC
authors: Pan
authors: Shanghai Kaibao Pharmaceutical Co. Ltd., Shanghai 201401, China.
authors: Wen-Ming
authors: WM
authors: Zhang
authors: Shanghai Kaibao Pharmaceutical Co. Ltd., Shanghai 201401, China. Electronic address: wshlwl@zju.edu.cn.
authors: Wen-Long
authors: WL
authors: Li
authors: Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China.
authors: Hai-Bin
authors: HB
authors: Qu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C540817:tanreqing
keywords: DAD; ESI-TOF/MS; Screening and identification; Tanreqing injection