title: Development of microsatellite markers of vandaceous orchids for species and variety identification.
abstract: Vandaceous orchids are a group of orchid genera in the subfamily Vandoideae. Among this group, Mokara, Phalaenopsis, and Vanda are the most popular and commercially important orchids in Thailand. Novel microsatellite markers were developed from Mokara, the intergeneric hybrid from 3 genera Vanda, Ascocentrum, and Arachnis by using enriched method. Six primers from this study plus one primer previously developed from Vanda genome, a total of 7 markers, were selected to characterize 4 orchid genera (Mokara, Vanda, Rhynchostylis, and Ascocenda). The observed and expected heterozygosities varied in the 4 genera from 0.0000-1.0000 and 0.0000-0.8765, respectively. The transferability of these primers was also investigated in 76 vandaceous orchids from 12 genera. Three primer pairs, MOK26, MOK29, and MOK62, could successfully amplify the DNA of all samples, while MOK103 could be used with most of the samples. The total number of alleles from 76 samples ranged from 3 to 19 alleles per locus, with an average of 8.5714. Therefore, these markers could be used for variety/ species identification, certification and protection, genetic diversity, and evolutionary studies.
authors: Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand fscisrp@ku.ac.th.
authors: S
authors: S
authors: Peyachoknagul
authors: Division of Molecular Medicine, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors: C
authors: C
authors: Nettuwakul
authors: Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors: P
authors: P
authors: Phuekvilai
authors: Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors: S
authors: S
authors: Wannapinpong
authors: Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors: K
authors: K
authors: Srikulnath
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000096:biosynthesis / Q000235:genetics*; D018744:DNA, Plant; D056426:Genetic Loci; D018745:Genome, Plant; D006579:Heterozygote; D018895:Microsatellite Repeats; D029595:Orchidaceae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D013045:Species Specificity; D013785:Thailand
chemical_list: D017931:DNA Primers; D018744:DNA, Plant