title: Quantitative expression of pluripotency-related genes in parthenogenetically produced buffalo (Bubalus bubalis) embryos and in putative embryonic stem cells derived from them.
abstract: Parthenogenetically produced embryos and embryonic stem (ES) cells derived from them offer a unique model for investigating the role of transcription factors in embryonic genome activation (EGA), pluripotent lineage specification and in pluripotency and self-renewal of ES cells because of the unique nature of these embryos. There is little information on the quantitative expression of important genes in parthenogenetically produced embryos and in ES cells derived from them. The present study examined the quantitative expression of some important genes in parthenogenetically produced buffalo embryos and in putative parthenogenetic ES cells (pES) cells. The quantitative expression of OCT-4, SOX-2, NANOG, REX-1, FOXD-3 and NUCLEOSTEMIN, which is very low in immature and mature oocytes, and in embryos at 2-, 4- and 8- to 16-cell stage, increases significantly at morula and blastocyst stage. The expression level of TELOMERASE, c-MYC and STAT-3, which is high in immature oocytes decreases during embryonic development followed by either an increase at the morula stage (TELOMERASE) or a low expression level maintained throughout development till blastocyst stage (c-MYC and STAT-3). There is a progressive decline in the expression level of OCT-4, SOX-2, c-MYC, REX-1, NUCLEOSTEMIN, TELOMERASE and STAT-3 during long term culture of pES cells.
authors: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors: K P
authors: KP
authors: Singh
authors: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors: R
authors: R
authors: Kaushik
authors: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors: S K
authors: SK
authors: Mohapatra
authors: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, India.
authors: V
authors: V
authors: Garg
authors: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors: K
authors: K
authors: Rameshbabu
authors: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors: M K
authors: MK
authors: Singh
authors: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors: P
authors: P
authors: Palta
authors: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors: R S
authors: RS
authors: Manik
authors: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors: S K
authors: SK
authors: Singla
authors: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India. Electronic address: chauhan_abtc@rediffmail.com.
authors: M S
authors: MS
authors: Chauhan
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002020:Buffaloes / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D047108:Embryonic Development; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D009028:Morula / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism; D010312:Parthenogenesis; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
keywords: Embryonic stem cells; Embryos; Parthenogenesis; Real time PCR