title: Production of a monoclonal antibody against serum immunoglobulin M of South American camelids and assessment of its suitability in two immunoassays.
abstract: A monoclonal antibody (mAb) was produced against immunoglobulin M (IgM) of South American camelids. A single radial immunodiffusion (SRID) assay and a competitive enzyme-linked immunosorbent assay (ELISA) were developed to measure IgM in serum samples. Isotype and specificity of the mAb were assessed. The performance of the SRID assay was preliminarily evaluated in terms of working range, plate stability over a 4-week period, and initial intra- and interassay variation. The concentration of IgM was determined in 55 samples by SRID assay and ELISA, and results were not significantly different by t-test (0.64 ± 0.19 mg/ml for the SRID assay, and 0.58 ± 0.24 mg/ml for ELISA; P = 0.1489). The mAb was shown to be stable over the 4-week evaluation period, and the SRID assay was reproducible when tested in triplicate for intra-assay variability and in quadruplicate for interassay variability, with a percentage coefficient of variation of less than or equal to 5%. Also, the SRID assay proved to be sensitive enough to measure IgM levels in undiluted serum samples, and had a good correlation with ELISA. The current study is intended to submit a preliminary report of a mAb against IgM of South American camelids, and suggest the future potential of the mAb developed for diagnostic application, including use in the SRID assay.
authors: Instituto de Estudios de la Inmunidad Humoral (IDEHU-CONICET-UBA), Buenos Aires, Argentina afriedrich.bioq@gmail.com.
authors: Adrián
authors: A
authors: Friedrich
authors: Instituto de Estudios de la Inmunidad Humoral (IDEHU-CONICET-UBA), Buenos Aires, Argentina.
authors: Martín
authors: M
authors: Ledesma
authors: Instituto de Estudios de la Inmunidad Humoral (IDEHU-CONICET-UBA), Buenos Aires, Argentina.
authors: Ignacio
authors: I
authors: Landone
authors: Instituto de Estudios de la Inmunidad Humoral (IDEHU-CONICET-UBA), Buenos Aires, Argentina.
authors: Alejandro
authors: A
authors: Ferrari
authors: Instituto de Estudios de la Inmunidad Humoral (IDEHU-CONICET-UBA), Buenos Aires, Argentina.
authors: Juliana
authors: J
authors: Leoni
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002161:Camelids, New World / Q000097:blood*; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D007075:Immunoglobulin M / Q000276:immunology*; D012680:Sensitivity and Specificity; D014481:United States
chemical_list: D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M
keywords: Immunoglobulin M; South American camelids; monoclonal antibodies