title: Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons.
abstract: Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified infectious RNA viruses were generated in days using the newly described ISA (infectious-subgenomic-amplicons) method. This new versatile and simple procedure may enhance our capacity to obtain infectious RNA viruses from PCR-amplified genetic material.
authors: Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 'Emergence des Pathologies Virales', 13385 Marseille, France.
authors: Fabien
authors: F
authors: Aubry
authors: Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 'Emergence des Pathologies Virales', 13385 Marseille, France.
authors: Antoine
authors: A
authors: Nougairède
authors: Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 'Emergence des Pathologies Virales', 13385 Marseille, France.
authors: Lauriane
authors: L
authors: de Fabritus
authors: Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 'Emergence des Pathologies Virales', 13385 Marseille, France.
authors: Gilles
authors: G
authors: Querat
authors: Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 'Emergence des Pathologies Virales', 13385 Marseille, France.
authors: Ernest A
authors: EA
authors: Gould
authors: Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 'Emergence des Pathologies Virales', 13385 Marseille, France.
authors: Xavier
authors: X
authors: de Lamballerie
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005416:Flavivirus / Q000235:genetics / Q000502:physiology; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D012328:RNA Viruses / Q000235:genetics* / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics; D059386:Reverse Genetics / Q000379:methods*; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral