title: Five-lineage clonal analysis of hematopoietic stem/progenitor cells.
abstract: Hematopoietic stem cells (HSCs) have self-renewal activity and multipotency. Clonal analysis and determination of HSC differentiation potential into platelets and erythrocytes as well as leukocytes are essential for the study of self-renewal and lineage commitment in HSC. However, due to technical limitations, platelet and erythrocyte differentiation potentials have not been assessed. This chapter describes principles and methods for single-cell sorting, single-cell transplantation, and identification and quantitative analysis of cell contribution to platelets and erythrocytes in addition to leukocytes in mouse chimeras.
authors: Department of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan.
authors: Ryo
authors: R
authors: Yamamoto
authors: Yohei
authors: Y
authors: Morita
authors: Hiromitsu
authors: H
authors: Nakauchi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D002999:Clone Cells / Q000166:cytology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D059010:Single-Cell Analysis / Q000379:methods*; D013194:Staining and Labeling