title: The effects of plant growth regulators and L-phenylalanine on phenolic compounds of sweet basil.
abstract: The effects of methyl jasmonate (MeJA), spermine (Spm), epibrassinolide (EBL) and l-phenylalanine on sweet basil (Ocimum basilicum L.) were studied to determine the amount of phenolic compounds and enzymatic activity of phenylalanine ammonia-lyase (PAL). Total phenolic and total flavonoid contents of sweet basils were determined by a spectrophotometer, and individual phenolic compounds and activity of PAL were analysed by HPLC/UV. The highest total phenolic (6.72 mg GAE/g) and total flavonoid contents (0.92 mg QE/g) obtained from 1.0 mM Spm+MeJA application. Rosmarinic acid (RA) and caffeic acid contents significantly enhanced after the applications but no such differences observed in chicoric acid content or PAL activity. RA was the main phenolic acid in all samples and its concentration varied from 1.04 to 2.70 mg/gFW. As a result the combinations of Spm+MeJA and EBL+MeJA can induce secondary metabolites effectively and those interactions play important role in the production of phytochemicals in plants.
authors: Department of Medicinal and Aromatic Plants, Turkoglu Vocational High School, Kahramanmaras Sutcu Imam University, 46100 Turkoglu-Kahramanmaras, Turkey. Electronic address: niluferkoca@ksu.edu.tr.
authors: Nülüfer
authors: N
authors: Koca
authors: Department of Biology, Faculty of Science, Kahramanmaras Sutcu Imam University, 46100 Avsar Campus-Kahramanmaras, Turkey.
authors: Şengül
authors: Ş
authors: Karaman
mesh_terms: D005419:Flavonoids / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis; D018649:Ocimum basilicum / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D010649:Phenylalanine / Q000494:pharmacology*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*
chemical_list: D005419:Flavonoids; D062385:Hydroxybenzoates; D010636:Phenols; D010937:Plant Growth Regulators; D010649:Phenylalanine; D010650:Phenylalanine Ammonia-Lyase; C017616:phenolic acid
keywords: 24-Epibrassinolide (PubChem CID 115196); Brassinosteroid; Caffeic acid (PubChem CID 689043); Chicoric acid (PubChem CID 5281764); MeJA; Methyl jasmonate (PubChem CID 5367719); Ocimum basilicum; Phenolic; Phenylalanine (PubChem CID 6140); Precursor; Rosmarinic acid (PubChem CID 5281792); Secondary metabolites; Spermine; Spermine (PubChem CID 1103)