title: Characterization of type II thioesterases involved in natamycin biosynthesis in Streptomyces chattanoogensis L10.
abstract: The known functions of type II thioesterases (TEIIs) in type I polyketide synthases (PKSs) include selecting of starter acyl units, removal of aberrant extender acyl units, releasing of final products, and dehydration of polyketide intermediates. In this study, we characterized two TEIIs (ScnI and PKSIaTEII) from Streptomyces chattanoogensis L10. Deletion of scnI in S. chattanoogensis L10 decreased the natamycin production by about 43%. Both ScnI and PKSIaTEII could remove acyl units from the acyl carrier proteins (ACPs) involved in the natamycin biosynthesis. Our results show that the TEII could play important roles in both the initiation step and the elongation steps of a polyketide biosynthesis; the intracellular TEIIs involved in different biosynthetic pathways could complement each other.
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Yue-Yue
authors: YY
authors: Wang
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Xin-Xin
authors: XX
authors: Ran
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Wei-Bin
authors: WB
authors: Chen
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Shui-Ping
authors: SP
authors: Liu
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Xiao-Sheng
authors: XS
authors: Zhang
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Yuan-Yang
authors: YY
authors: Guo
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors: Xin-Hang
authors: XH
authors: Jiang
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China,; Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, Zhejiang 310058, China. Electronic address: jianghuisioc@zjuedu.cn.
authors: Hui
authors: H
authors: Jiang
authors: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China,; Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, Zhejiang 310058, China. Electronic address: lyq@zju.edu.cn.
authors: Yong-Quan
authors: YQ
authors: Li
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000737:chemistry / Q000378:metabolism*; D042541:Intracellular Space / Q000201:enzymology; D008969:Molecular Sequence Data; D010866:Natamycin / Q000096:biosynthesis*; D013302:Streptomyces / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010866:Natamycin; D001120:Arginine; D064429:Fatty Acid Synthases; D013869:Thiolester Hydrolases; C022738:thioesterase II
keywords: Biosynthesis; Natamycin; Polyketide synthase; Thioesterase