title: Expression of key hydrolases for soy sauce fermentation in Zygosaccharomyces rouxii.
abstract: Several key hydrolases in soy sauce fermentation such as proteases, peptidases, and glutaminases are supplied by Aspergillus sojae or Aspergillus oryzae. The genes encoding these hydrolases were successfully expressed in salt-tolerant yeast Zygosaccharomyces rouxii. These transformants are expected to supply extra hydrolases during soy sauce fermentation process.
authors: Research & Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba Pref. 278-0037, Japan. Electronic address: myuzuki@mail.kikkoman.co.jp.
authors: Masanobu
authors: M
authors: Yuzuki
authors: Research & Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba Pref. 278-0037, Japan.
authors: Kenichiro
authors: K
authors: Matsushima
authors: Research & Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba Pref. 278-0037, Japan.
authors: Yasuji
authors: Y
authors: Koyama
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism; D004150:Dipeptidases / Q000235:genetics / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D005285:Fermentation / Q000235:genetics; D005972:Glutaminase / Q000235:genetics / Q000378:metabolism; D006867:Hydrolases / Q000235:genetics* / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism; D045730:Soy Foods; D020068:Zygosaccharomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012965:Sodium Chloride; D006867:Hydrolases; D002268:Carboxypeptidases; D010450:Endopeptidases; D004150:Dipeptidases; C022449:proline dipeptidase; C087822:alkaline protease; D005972:Glutaminase
keywords: Alkaline protease; Carboxypeptidase; Gene expression; Glutaminase; Neutral protease; Prolidase; Zygosaccharomyces rouxii