title: Effects of nonylphenol and ethinylestradiol on copper redhorse (Moxostoma hubbsi), an endangered species.
abstract: The copper redhorse, Moxostoma hubbsi, is an endangered species endemic to Quebec. The presence of contaminants, in particular endocrine disrupting chemicals (EDCs), in its habitat has been advanced as partly responsible for the reproductive difficulties encountered by the species. In the present study, immature copper redhorse were exposed to the estrogenic surfactant nonylphenol (NP; 1, 10 and 50µg/l) and the synthetic estrogen 17α-ethinylestradiol (EE2; 10ng/l) for 21 days in a flow-through system. The endpoints investigated included general health indicators (hepatosomatic index and hematocrit), thyroid hormones, sex steroids, brain aromatase activity, plasma and mucus vitellogenin (VTG), cytochrome P4501A protein expression and ethoxyresorufin-O-deethylase activity, heat shock protein 70 (HSP70) and muscle acetylcholinesterase. Exposure to 10ng EE2/l significantly increased brain aromatase activity. Exposure to 50µg NP/l resulted in a significant reduction of plasma testosterone concentrations and a significant induction of hepatic HSP70 protein expression. NP at 50µg/l also induced plasma and mucus VTG. The presence of elevated VTG levels in the surface mucus of immature copper redhorse exposed to NP, and its correlation to plasma VTG, supports the use of mucus VTG as a non-invasive biomarker to evaluate copper redhorse exposure to EDCs in the environment and contribute to restoration efforts of the species. The results of the present study indicate that exposure to high environmentally relevant concentrations of NP and EE2 can affect molecular endpoints related to reproduction in the copper redhorse.
authors: Pêches et Océans Canada, Institut Maurice-Lamontagne, 850 route de la Mer, Mont-Joli, QC, Canada G5H 3Z4. Electronic address: domynick.maltais@dfo-mpo.gc.ca.
authors: Domynick
authors: D
authors: Maltais
authors: Pêches et Océans Canada, Institut Maurice-Lamontagne, 850 route de la Mer, Mont-Joli, QC, Canada G5H 3Z4.
authors: Robert L
authors: RL
authors: Roy
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism; D003531:Cypriniformes / Q000097:blood*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D056727:Endangered Species; D052244:Endocrine Disruptors / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000633:toxicity*; D012739:Gonadal Steroid Hormones / Q000097:blood; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006400:Hematocrit; D008099:Liver / Q000201:enzymology; D009093:Mucus / Q000737:chemistry; D010636:Phenols / Q000633:toxicity*; D011792:Quebec; D011897:Random Allocation; D013963:Thyroid Hormones / Q000097:blood; D014819:Vitellogenins / Q000097:blood
chemical_list: D052244:Endocrine Disruptors; D012739:Gonadal Steroid Hormones; D018840:HSP70 Heat-Shock Proteins; D010636:Phenols; D013963:Thyroid Hormones; D014819:Vitellogenins; D004997:Ethinyl Estradiol; C025256:nonylphenol; D001141:Aromatase; D019363:Cytochrome P-450 CYP1A1
keywords: Aromatase; Copper redhorse; Endocrine disrupting chemicals; Heat shock protein 70; Steroid hormones; Vitellogenin