title: Dynamic profile and adipogenic role of growth differentiation factor 5 (GDF5) in the differentiation of 3T3-L1 preadipocytes.
abstract: Adipocyte differentiation is key to determining the number of adipocytes during the development of obesity. Recent studies have shown that growth differentiation factor-5 (GDF5) promotes brown adipogenesis, however its role in white adipogenesis is still uncertain. The aim of the present study was to investigate the effect of GDF5 on white adipogenesis using 3T3-L1 preadipocyte model. In the present study, GDF5 was found to be differentially regulated during adipocyte differentiation. GDF5 protein increased the differentiation of 3T3-L1 preadipocytes, especially when these cells were exposed to hormone cocktails without insulin. During adipogenesis, GDF5 enhanced the expression of genes related to adipocyte differentiation and caused cells to enter the S phase. Short-hairpin-RNA knockdown of GDF5 in 3T3-L1 cells was found to prevent adipogenesis induced by a standard hormone cocktail and to downregulate the expression of adipocyte genes and proteins, this impairment could be partly rescued by GDF5 protein. Collectively, these results suggest that GDF5 can promote progression of the cell-cycle and increase numbers of cells in S phase, GDF5 might play a critical role in 3T3-L1 preadipocyte differentiation.
authors: Department of Pediatric Endocrinology and Inherited Metabolic Disease, Children's Hospital of Fudan University, Shanghai, China.
authors: Zhou
authors: Z
authors: Pei
authors: Institute of Pediatrics, Children's Hospital of Fudan University, Shanghai, China.
authors: Yi
authors: Y
authors: Yang
authors: Department of Women and Child Health, Hospital for Children and Adolescents, Center for Pediatric Research, University of Leipzig, Leipzig, Germany.
authors: Wieland
authors: W
authors: Kiess
authors: Department of Pediatric Endocrinology and Inherited Metabolic Disease, Children's Hospital of Fudan University, Shanghai, China; Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden.
authors: Chengjun
authors: C
authors: Sun
authors: Department of Pediatric Endocrinology and Inherited Metabolic Disease, Children's Hospital of Fudan University, Shanghai, China. Electronic address: luofh@fudan.edu.cn.
authors: Feihong
authors: F
authors: Luo
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D052438:Adipocytes, White / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D055428:Growth Differentiation Factor 5 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D047493:PPAR alpha / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D052242:Adiponectin; D022763:CCAAT-Enhancer-Binding Protein-alpha; C493411:Fabp4 protein, mouse; D050556:Fatty Acid-Binding Proteins; D055428:Growth Differentiation Factor 5; D047493:PPAR alpha; D012333:RNA, Messenger
keywords: Adipocyte; Differentiation; Growth differentiation factor 5; Preadipocytes