title: Enhanced rosmarinic acid biosynthesis in Solenostemon scutellarioides culture: a precursor-feeding strategy.
abstract: The aim of this study was to employ precursor-feeding strategy for the improved production of rosmarinic acid (RA) in Solenostemon scutellarioides in vitro. The cultures were fed with precursors, namely l-phenylalanine (Phe), l-tyrosine (Tyr) and cucumber juice (CJ), at different concentrations. Phe (100 mg L(-1)) and Tyr (400 mg L(-1)) caused ∼1.5- and 2.1-fold increase in RA accumulation within 48 h. CJ (50 mg L(-1)) feeding displayed highest RA content (∼1.6-fold) in 72 h. In this study, we focused on the function of individual precursor on key enzymes involved in RA biosynthesis. The phenylalanine ammonia lyase activity was significantly upregulated after Phe (100 mg L(-1)) feeding, while tyrosine aminotransferase and hydroxyphenylpyruvate reductase activities were improved with Tyr (400 mg L(-1)) treatment. However, rosmarinic acid synthase activity was significantly enhanced by all three precursors. In synergy study, Phe (100 mg L(-1)) + Tyr (400 mg L(-1)) could enhance (∼3.1-fold) RA biosynthesis within 48 h.
authors: a Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology , Jadavpur University , Raja S. C. Mullik Road, Kolkata , 700032 , West Bengal India.
authors: Saikat
authors: S
authors: Dewanjee
authors: Moumita
authors: M
authors: Gangopadhyay
authors: Urmi
authors: U
authors: Das
authors: Ranabir
authors: R
authors: Sahu
authors: Ritu
authors: R
authors: Khanra
mesh_terms: D002934:Cinnamates / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D053630:Depsides / Q000378:metabolism*; D019686:Lamiaceae / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D046509:Tissue Culture Techniques; D014444:Tyrosine Transaminase / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D003470:Culture Media; D053630:Depsides; D010088:Oxidoreductases; D010650:Phenylalanine Ammonia-Lyase; D014444:Tyrosine Transaminase; D000097123:Rosmarinic Acid
keywords: Solenostemon scutellarioides; biosynthetic enzymes; precursors; rosmarinic acid