title: Immobilization of phenylalanine-dehydrogenase on nano-sized polytaurine: a new platform for application of nano-polymeric materials on enzymatic biosensing technology.
abstract: A strategy of phenylalanine-dehydrogenase (PheDH) entrapment within the polytaurine matrix is demonstrated to probe the direct electrochemistry of phenylalanine (Pha). It was found that PheDH has been stably immobilized on glassy carbon electrode modified by polytaurine based on simple technique. Cyclic voltammetric study indicated that the oxidation process is irreversible and diffusion controlled. The number of exchanged electrons in the electro-oxidation process was obtained, and the data indicated that Pha is oxidized via one-electron steps. The results revealed that Pha promotes the rate of oxidation by increasing the peak current. The diffusion coefficient and electron-transfer coefficient of Pha were found to be 0.2×10(-6)cm(2)s(-1) and 0.467, respectively. A sensitive, simple and time-saving differential-pulse voltammetric procedure was developed for the analysis of Pha. The results show that by using the proposed method, Pha can be determined with a detection limit of 9 nM.
authors: Enzyme Technology Lab., Biochemistry Dept., Genetic and Metabolism Group, Pasteur Institute of Iran, P.O. Box 13164, Tehran, Iran. Electronic address: skandar@pasteur.ac.ir.
authors: Eskandar
authors: E
authors: Omidinia
authors: Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran. Electronic address: nasrin.shadjou@gmail.com.
authors: Nasrin
authors: N
authors: Shadjou
authors: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran. Electronic address: mhmmd_hasanzadeh@yahoo.com.
authors: Mohammad
authors: M
authors: Hasanzadeh
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D010649:Phenylalanine / Q000032:analysis* / Q000378:metabolism; D013654:Taurine / Q000031:analogs & derivatives / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D013654:Taurine; D010649:Phenylalanine; D010088:Oxidoreductases
keywords: Enzyme nanobiosensing; Nano-polymers; Phenylalanine-dehydrogenase; Polytaurine