title: Co-metabolic degradation of tetrabromobisphenol A by novel strains of Pseudomonas sp. and Streptococcus sp.
abstract: Three strains capable of rapidly degrading TBBPA by co-metabolism and utilizing formate as the carbon source, named as J-F-01, J-F-02, and J-F-03, respectively, were isolated from enrichment cultures, which have been treated with 0.5mg/L TBBPA for 240 d. Based on morphology and 16S rRNA gene sequence analysis, both J-F-01 and J-F-02 were determined to Pseudomonas sp., while J-F-03 was identified as Streptococcus sp. A shorter half-life (6.1d) of TBBPA was observed in pure culture of J-F-03 when compared with J-F-01 (22.5d) and J-F-02 (13.6d). Surprisingly, the degradation of TBBPA was significantly enhanced by the mixed culture of J-F-02 and J-F-03. The optimal degradation conditions for the mixed cultures were determined. Under the optimal conditions, TBBPA (0.5mg/L) was completely metabolized by the mixed culture within ten days. Moreover, bromide and the metabolisms were detected, and a possible metabolic pathway was deduced from the detection of metabolite production patterns.
authors: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510275, China.
authors: Xingxing
authors: X
authors: Peng
authors: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510275, China.
authors: Xiangdong
authors: X
authors: Qu
authors: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510275, China.
authors: Weishi
authors: W
authors: Luo
authors: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510275, China. Electronic address: eesjxs@mail.sysu.edu.cn.
authors: Xiaoshan
authors: X
authors: Jia
mesh_terms: D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D001965:Bromides / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D010802:Phylogeny; D011075:Polybrominated Biphenyls / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001965:Bromides; D005561:Formates; D011075:Polybrominated Biphenyls; D012336:RNA, Ribosomal, 16S; C030544:formic acid; C020806:tetrabromobisphenol A
keywords: Co-metabolism; Degradation; Formate; TBBPA