title: RapidBurkholderia pseudomalleiidentification and antibiotic resistance determination by bacteriophage amplification and MALDI-TOF MS.
abstract: Phage amplification detected by MALDI-TOF MS was investigated for rapid and simultaneousBurkholderia pseudomalleiidentification and ceftazidime resistance determination.B. pseudomalleiceftazidime susceptible and resistant ΔpurMmutant strains Bp82 and Bp82.3 were infected with broadly targetingB. pseudomalleiphage ϕX216 and production of the m/z 37.6 kDa phage capsid protein observed by MALDI-TOF MS over the course of 3 h infections. This allowed for repoducible phage-based bacterial ID within 2 h of the onset of infection. MALDI-TOF MS-measured time to detection correlated with in silico modeling, which predicted an approximate 2 h detection time. Ceftazidime susceptible strain Bp82, while detectable in the absence of the drug, owing to the reliance of phage amplification on a viable host, was not detectable when 10 μg/mL ceftazidime was added at the onset of infection. In contrast, resistant strain Bp82.3 was detected in the same 2 h timeframe both with and without the addition of ceftazidime.
authors: Department of Chemistry and Geochemistry; Colorado School of Mines; Golden, CO USA.
authors: Christopher R
authors: CR
authors: Cox
authors: Department of Chemistry and Geochemistry; Colorado School of Mines; Golden, CO USA.
authors: Nicholas R
authors: NR
authors: Saichek
authors: Department of Microbiology, Immunology and Pathology; Colorado State University; IDRC at Foothills Campus; Fort Collins, CO USA.
authors: Herbert P
authors: HP
authors: Schweizer
authors: Department of Chemistry and Geochemistry; Colorado School of Mines; Golden, CO USA.
authors: Kent J
authors: KJ
authors: Voorhees
keywords: Burkholderia; MALDI-TOF MS; antibiotic resistance; bacterial identification; phage amplification