title: Stanniocalcin-1 promotes metastasis in a human breast cancer cell line through activation of PI3K.
abstract: Stanniocalcin-l (STC-1) is a secreted glycoprotein hormone that regulates calcium and phosphate homeostasis. STC-1 expression is upregulated in several cancers including breast cancer, and has been shown to be prognostic. Although these clinical observations implicate STC-1 as a potential tumor marker, it is still unclear whether STC-1 confers a malignant phenotype. In this study, this question was addressed by overexpressing STC-1 in the human breast cancer cell line MDA-MB-231 and examining the resultant phenotype in vitro and in vivo. Overexpression of STC-1 enhanced invasiveness of MDA-MB-231 cells in vitro and promoted their lung metastasis in vivo, while having no effect on proliferation, adhesion, or proteinase activity. The addition of soluble STC-1 to MDA-MB-231 cultures resulted in the activation of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway, suggesting a mechanistic basis for the observed increases in cell motility and metastasis. Taken together, it was indicated that secreted STC-1 promotes metastatic potential of breast cancer cells via activation of PI3K/AKT.
authors: Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo, 060-8543, Japan.
authors: Ryosei
authors: R
authors: Murai
authors: Maki
authors: M
authors: Tanaka
authors: Yusuke
authors: Y
authors: Takahashi
authors: Kageaki
authors: K
authors: Kuribayashi
authors: Daisuke
authors: D
authors: Kobayashi
authors: Naoki
authors: N
authors: Watanabe
mesh_terms: D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D017931:DNA Primers; D004789:Enzyme Activation; D005260:Female; D006023:Glycoproteins / Q000502:physiology*; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary; D051379:Mice; D009362:Neoplasm Metastasis; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism
chemical_list: D017931:DNA Primers; D006023:Glycoproteins; C051795:teleocalcin; D019869:Phosphatidylinositol 3-Kinases