title: An ARID domain-containing protein within nuclear bodies is required for sperm cell formation in Arabidopsis thaliana.
abstract: In plants, each male meiotic product undergoes mitosis, and then one of the resulting cells divides again, yielding a three-celled pollen grain comprised of a vegetative cell and two sperm cells. Several genes have been found to act in this process, and DUO1 (DUO POLLEN 1), a transcription factor, plays a key role in sperm cell formation by activating expression of several germline genes. But how DUO1 itself is activated and how sperm cell formation is initiated remain unknown. To expand our understanding of sperm cell formation, we characterized an ARID (AT-Rich Interacting Domain)-containing protein, ARID1, that is specifically required for sperm cell formation in Arabidopsis. ARID1 localizes within nuclear bodies that are transiently present in the generative cell from which sperm cells arise, coincident with the timing of DUO1 activation. An arid1 mutant and antisense arid1 plants had an increased incidence of pollen with only a single sperm-like cell and exhibited reduced fertility as well as reduced expression of DUO1. In vitro and in vivo evidence showed that ARID1 binds to the DUO1 promoter. Lastly, we found that ARID1 physically associates with histone deacetylase 8 and that histone acetylation, which in wild type is evident only in sperm, expanded to the vegetative cell nucleus in the arid1 mutant. This study identifies a novel component required for sperm cell formation in plants and uncovers a direct positive regulatory role of ARID1 on DUO1 through association with histone acetylation.
authors: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China; Plant Gene Expression Center, USDA/ARS and Dept. of Plant and Microbial Biology, UC-Berkeley, Albany, California, United States of America.
authors: Binglian
authors: B
authors: Zheng
authors: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China.
authors: Hui
authors: H
authors: He
authors: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China.
authors: Yanhua
authors: Y
authors: Zheng
authors: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China.
authors: Wenye
authors: W
authors: Wu
authors: Plant Gene Expression Center, USDA/ARS and Dept. of Plant and Microbial Biology, UC-Berkeley, Albany, California, United States of America.
authors: Sheila
authors: S
authors: McCormick
mesh_terms: D000107:Acetylation; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics; D005854:Germ Cells / Q000378:metabolism; D006655:Histone Deacetylases; D006657:Histones / Q000235:genetics / Q000378:metabolism; D008540:Meiosis / Q000235:genetics*; D008938:Mitosis / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D011058:Pollen / Q000166:cytology* / Q000254:growth & development; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: C000595118:ARID1 protein, Arabidopsis; D029681:Arabidopsis Proteins; C499555:DUO1 protein, Arabidopsis; D006657:Histones; D009687:Nuclear Proteins; D014157:Transcription Factors; D006655:Histone Deacetylases