title: Evaluation of analgesic, anti-inflammatory and CNS depressant activities of methanolic extract of Lawsonia inermis barks in mice.
abstract: OBJECTIVE
  The study was carried out to assess the analgesic, anti-inflammatory, and CNS depressant activity of the methanolic extract of the Lawsonia inermis barks (MELIB).
  
  
  METHODS
  Anti-inflammatory effects of MEBLI were studied using carrageenan-induced inflammatory method at the dose of 300 and 500 mg/kg b.wt., p.o. Analgesic activity was measured using acetic acid-induced writhing model and formalin-induced licking and biting in mice. The CNS depressant activity was evaluated by observing the reduction of locomotor and exploratory activities in the open field and hole cross tests at a dose of 300 and 500 mg/kg body weight.
  
  
  RESULTS
  Statistical analysis showed that dose of 500 mg/kg exhibited higher analgesic activity against acetic acid-induced pain in mice than the standard drug diclofenac sodium. Furthermore, doses of 300 and 500 mg/kg caused higher percent of protection (91.16% and 95.03%, respectively) of licking and biting of formalin-induced mice than diclophenac sodium (70.72%). The Lawsonia inemis methanolic extract (300 and 500 mg/kg) also exhibited sustained inhibition (54.97% and 65.56%) of paw edema at the 4(th) hour compared with standard indomethacin (74.17%). Besides, the plant extract also had significant (p<0.05) dose-dependent CNS depressant activity.
  
  
  CONCLUSIONS
  this study recommends that the methanolic extract of Lawsonia inermis barks has significant analgesic, anti-inflammatory, and CNS depressant properties.
authors: Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh.
authors: Luthfun
authors: L
authors: Nesa
authors: Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh.
authors: Shirajum
authors: S
authors: Munira
authors: Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh.
authors: Shabnam
authors: S
authors: Mollika
authors: Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh.
authors: Monirul
authors: M
authors: Islam
authors: Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh.
authors: Habibullah
authors: H
authors: Choin
authors: Department of Pharmacy, University of Rajshahi, Bangladesh.
authors: Aktar Uzzaman
authors: AU
authors: Chouduri
authors: Radiant Pharmaceutical Ltd, Tongi, Bangladesh.
authors: Nazmun
authors: N
authors: Naher
keywords: Analgesic; Anti-inflammatory; CNS depressant; Carrageenan; Formalin induced pain Methanolic extract of Lawsonia inermis barks; Writhing