title: Assessment of immature platelet fraction and immature reticulocyte fraction as predictors of engraftment after hematopoietic stem cell transplantation.
abstract: BACKGROUND
  Engraftment is a critical milestone of the hematopoietic stem cell transplantation (HSCT) process. The immature platelet fraction (IPF) and immature reticulocyte fraction (IRF) are considered early indicators of bone marrow recovery. The objective of this study was to assess these parameters as predictors of HSCT engraftment.
  
  
  METHODS
  Neutrophil and platelet engraftment were defined as the first of three consecutive days with an absolute neutrophil count >0.5 × 10(9) /L or platelet count >20 × 10(9) /L, respectively. The IRF cutoff was 12%. Two IPF cutoffs were used: >6.2% and >10%.
  
  
  RESULTS
  The study sample comprised 44 patients, of whom 24 had undergone autologous HSCT and 20 had undergone allogeneic HSCT. Absolute neutrophil counts >0.5 × 10(9) /L were preceded by IRF >12% in 86% of patients (38 of 44). Platelet counts >20 × 10(9) /L were preceded by an IPF >6.2% in 90% of patients (37 of 41) and by an IPF >10% in 63% of patients (26 of 41).
  
  
  CONCLUSIONS
  The results show that IRF and IPF are engraftment predictors. Peak in IPF was observed before rise in platelet count, while IRF rises before absolute neutrophil count (ANC) and persists increased. This indicates that IRF and IPF can be considered as new tools for hematopoietic assessment after HSCT.
authors: Multidisciplinary Residency Program in Oncology and Hematology, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
authors: I V C
authors: IV
authors: Morkis
authors: M G
authors: MG
authors: Farias
authors: L D C
authors: LD
authors: Rigoni
authors: L
authors: L
authors: Scotti
authors: L J
authors: LJ
authors: Gregianin
authors: L E
authors: LE
authors: Daudt
authors: L M da R
authors: LM
authors: Silla
authors: A A
authors: AA
authors: Paz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005260:Female; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007223:Infant; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009504:Neutrophils; D010976:Platelet Count; D011379:Prognosis; D017701:Reticulocyte Count; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D055815:Young Adult
keywords: Hematopoietic stem cell transplantation; bone marrow cell transplantation; bone marrow grafting; immature platelet fraction; immature reticulocyte fraction