title: Fatty acid composition, antioxidant and antibacterial activities of Adonis wolgensis L. extract.
abstract: OBJECTIVE
  The objective of this study was to analyze the fatty acid content, antioxidant, and antibacterial activities of hydro-methanolic extract of Adonis wolgensis L. (A. wolgensis L.) growing wild in north of Iran.
  
  
  METHODS
  Oils of A. wolgensis L. was obtained by means of Soxhlet apparatus from leaves and stems. Methyl esters were derived from the oily mixtures by trans-esterification process and were analyzed by GC/FID and GC/MS systems. Phenolic compounds extraction was done with aqueous methanol (90%). This extract was investigated for antioxidant activity using DPPH radical scavenging and reducing power methods and was also tested against a panel of microorganisms.
  
  
  RESULTS
  Linolenic acid (45.83%) and oleic acid (47.54%) were the most abundant fatty acids in leaves and stems, respectively. Hydro-methanolic extract with the high amount of total phenolics (9.20 ±0.011 mg GAE per dry matter) was the potent antioxidant in the assays. RESULTS obtained from measurements of MIC for extract, indicated that E. coli, S. aureus, and S. enteritidis were the most sensitive microorganisms tested, but no activity was observed against Gram-positive microorganism (B. subtilis).
  
  
  CONCLUSIONS
  The results obtained from the present study indicated that the oil of A. wolgensis leaves and stems contained a high source of poly-unsaturated fatty acids (PUFAs). These results also showed that hydro-methanolic extract of this plant contained significant antioxidant and antibacterial activities.
authors: Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, I. R. Iran.
authors: Maryam
authors: M
authors: Mohadjerani
authors: Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran , Babolsar, I. R. Iran ; Department of Medical Engineering, Maziar University, Noor, I. R. Iran.
authors: Rahmatollah
authors: R
authors: Tavakoli
authors: Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran , Babolsar, I. R. Iran.
authors: Rahman
authors: R
authors: Hosseinzadeh
keywords: Adonis wolgensis; Antibacterial activity; Antioxidant activity; Fatty acid