title: [Proteome mass-spectrometric analysis and typing of Vibrio cholerae strains isolated in the territory of Russian Federation in 2010 - 2012].
abstract: OBJECTIVE
  Conduction of a comparative proteomic mass-spectrometric (MS) analysis using a personal database of V. cholerae protein mass-spectra and genetic VNTR-typing of cholera causative agent strains.
  
  
  METHODS
  V. cholerae O1 El Tor strains - 7, V. cholerae non O1/non O139 - 2. Protein profiling and VNTR-genotyping of strains was carried out on MALDI TOF-MS Autoflex (Bruker Daltonics) mass-spectrometer and SIS Cholera-strains-VNTR.
  
  
  RESULTS
  The established community of a proteomic profile of epidemic cholera vibrio strains isolated in 2010 - 2012 in Moscow allowed to determine Indian origin of a toxigenic strain isolated in Taganrog in 2011. M/z proteins distinguishing V. cholerae O1 and non O1/non O139 strains were identified. Proteomic analysis confirms the results of VNTR-genotyping.
  
  
  CONCLUSIONS
  Study and typing of V. cholerae members with determination of their origin and phylogenetic relationship is possible using a collection of V. cholerae mass-spectra.
authors: N R
authors: NR
authors: Telesmanich
authors: V V
authors: VV
authors: Agafonova
authors: O S
authors: OS
authors: Chemisova
authors: I A
authors: IA
authors: Chaĭka
authors: A S
authors: AS
authors: Vodop'ianov
authors: E V
authors: EV
authors: Goncharenko
authors: V O
authors: VO
authors: Telicheva
mesh_terms: D002771:Cholera / Q000453:epidemiology / Q000382:microbiology; D003470:Culture Media; D036781:Fimbriae Proteins / Q000145:classification / Q000235:genetics*; D015870:Gene Expression; D056426:Genetic Loci; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D058885:Multilocus Sequence Typing / Q000379:methods; D010802:Phylogeny; D058974:Phylogeography; D016133:Polymerase Chain Reaction; D040901:Proteomics; D012426:Russia / Q000453:epidemiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; C085807:TcpA protein, Vibrio cholerae; D036781:Fimbriae Proteins