title: Quantification of photocyanine in human serum by high-performance liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study.
abstract: Photocyanine is a novel anticancer drug. Its pharmacokinetic study in cancer patients is therefore very important for choosing doses, and dosing intervals in clinical application. A rapid, selective and sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the determination of photocyanine in patient serum. Sample preparation involved one-step protein precipitation by adding methanol and N,N-dimethyl formamide to 0.1 mL serum. The detection was performed on a triple quadrupole tandem mass spectrometer operating in multiple reaction-monitoring (MRM) mode. Each sample was chromatographed within 7 min. Linear calibration curves were obtained for photocyanine at a concentration range of 20-2000 ng/mL (r > 0.995), with the lower limit of quantification (LLOQ) being 20 ng/mL. The intrabatch accuracy ranged from 101.98% to 107.54%, and the interbatch accuracy varied from 100.52% to 105.62%. Stability tests showed that photocyanine was stable throughout the analytical procedure. This study is the first to utilize the HPLC-MS/MS method for the pharmacokinetic study of photocyanine in six cancer patients who had received a single dose of photocyanine (0.1 mg/kg) administered intravenously.
authors: State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China ; Department of Clinical Trial Center, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China.
authors: Bing-Tian
authors: BT
authors: Bi
authors: State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China ; Department of Medical Oncology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China.
authors: Ben-Yan
authors: BY
authors: Zou
authors: State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China ; Department of Clinical Trial Center, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China.
authors: Li-Ting
authors: LT
authors: Deng
authors: State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China ; Department of Clinical Trial Center, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China.
authors: Jing
authors: J
authors: Zhan
authors: State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China ; Department of Clinical Trial Center, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China.
authors: Hai
authors: H
authors: Liao
authors: State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China ; Department of Clinical Trial Center, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China.
authors: Kun-Yao
authors: KY
authors: Feng
authors: State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China ; Department of Clinical Trial Center, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China.
authors: Su
authors: S
authors: Li