title: Biomimetic production of silk-like recombinant squid sucker ring teeth proteins.
abstract: The sucker ring teeth (SRT) of Humboldt squid exhibit mechanical properties that rival those of robust engineered synthetic polymers. Remarkably, these properties are achieved without a mineral phase or covalent cross-links. Instead, SRT are exclusively made of silk-like proteins called "suckerins", which assemble into nanoconfined β-sheet reinforced supramolecular networks. In this study, three streamlined strategies for full-length recombinant suckerin protein production and purification were developed. Recombinant suckerin exhibited high solubility and colloidal stability in aqueous-based solvents. In addition, the colloidal suspensions exhibited a concentration-dependent conformational switch, from random coil to β-sheet enriched structures. Our results demonstrate that recombinant suckerin can be produced in a facile manner in E. coli and processed from mild aqueous solutions into materials enriched in β-sheets. We suggest that recombinant suckerin-based materials offer potential for a range of biomedical and engineering applications.
authors: School of Materials Science and Engineering, Nanyang Technological University , 50 Nanyang Avenue, Singapore 639798.
authors: Dawei
authors: D
authors: Ding
authors: Paul A
authors: PA
authors: Guerette
authors: Shawn
authors: S
authors: Hoon
authors: Kiat Whye
authors: KW
authors: Kong
authors: Tobias
authors: T
authors: Cornvik
authors: Martina
authors: M
authors: Nilsson
authors: Akshita
authors: A
authors: Kumar
authors: Julien
authors: J
authors: Lescar
authors: Ali
authors: A
authors: Miserez
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D049832:Decapodiformes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D047011:Silk; D014070:Tooth / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D047011:Silk