title: Phytochemicals from Kaempferia angustifolia Rosc. and their cytotoxic and antimicrobial activities.
abstract: Phytochemical investigation on rhizomes of Kaempferia angustifolia has afforded a new abietene diterpene, kaempfolienol (1) along with crotepoxide (2), boesenboxide (3), 2'-hydroxy-4,4',6'-trimethoxychalcone (4), zeylenol (5), 6-methylzeylenol (6), (24S)-24-methyl-5α-lanosta-9(11), 25-dien-3β-ol (7), sucrose, β-sitosterol, and its glycoside (8). The structures of the compounds were elucidated on the basis of spectroscopic methods (IR, MS, and NMR). Isolation of 6-methylzeylenol (6), (24S)-24-methyl-5α-lanosta-9(11), 25-dien-3β-ol (7), and β-sitosterol-3-O-β-D-glucopyranoside (8) from this plant species has never been reported previously. The spectroscopic data of (7) is firstly described in this paper. Cytotoxic screening indicated that most of the pure compounds tested showed significant activity with (4) showing the most potent activity against HL-60 (human promyelocytic leukemia) and MCF-7 (human breast cancer) cell lines. However, all extracts and most of the pure compounds tested were found to be inactive against HT-29 (human colon cancer) and HeLa (human cervical cancer) cell lines. Similarly, none of the extracts or compounds showed activity in the antimicrobial testing.
authors: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: Sook Wah
authors: SW
authors: Tang
authors: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: Mohd Aspollah
authors: MA
authors: Sukari
authors: Sime Darby Technology Center, 2 Jalan Tandang, 46050 Petaling Jaya, Selangor, Malaysia.
authors: Bee Keat
authors: 0000-0002-6973-696X
authors: BK
authors: Neoh
authors: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: Yunie Soon Yu
authors: YS
authors: Yeap
authors: UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: Ahmad Bustamam
authors: AB
authors: Abdul
authors: Pangiran Anak Puteri Rashidah Sa'adatul Bolkiah Institute of Health Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong BE1410, Brunei Darussalam.
authors: Nurolaini
authors: 0000-0002-8817-7956
authors: N
authors: Kifli
authors: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: Gwendoline Cheng Lian
authors: GC
authors: Ee
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013696:Temperature; D030521:Zingiberaceae / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts