title: Estimation of vascular endothelial growth factor gene -460 C/T polymorphism as a biomarker in oral squamous cell carcinoma patients from the Indian subcontinent.
abstract: OBJECTIVE
  Oral squamous cell carcinoma (OSCC) is one of the most common malignancies in India. Vascular endothelial growth factor (VEGF), a major mediator of angiogenesis, is known to play a key role in the pathophysiology of tumorigenesis. Polymorphisms of the VEGF gene have been shown to influence the risk of OSCC, but it remains to be elucidated in the Indian population. Thus, the present study was designed to detect the VEGF gene -460 C/T polymorphism in OSCC patients from the Indian population, which is genetically distinct from previously-studied populations.
  
  
  METHODS
  Peripheral blood samples of 80 OSCC patients and 80 healthy individuals were collected for a case-control study design. The VEGF gene -460 C/T polymorphism was detected by polymerase chain reaction-based restriction analysis.
  
  
  RESULTS
  The analysis revealed significant differences between normal individuals and patients with OSCC (P < 0.05). The distribution of the TT homozygote and the T allele in the patient group was significantly greater than in the control group.
  
  
  CONCLUSIONS
  The present study provides novel evidence of the role of the VEGF gene -460 C/T polymorphism as a suitable genetic marker of OSCC in the Indian population.
authors: Department of Dentistry, Shri Bhausaheb Hire Government Medical College and Hospital, Dhule, Maharashtra, India.
authors: Archana P
authors: AP
authors: Borase
authors: Department of Oral Pathology and Microbiology, Government Dental College and Hospital, Nagpur, Maharashtra, India.
authors: Sindhu M
authors: SM
authors: Ganvir
authors: Department of Oral Pathology and Microbiology, Government Dental College and Hospital, Nagpur, Maharashtra, India.
authors: Vinay K
authors: VK
authors: Hazarey
authors: Department of Oral Pathology and Microbiology, Government Dental College and Hospital, Nagpur, Maharashtra, India.
authors: Suchitra R
authors: SR
authors: Gosavi
authors: Department of Conservative Dentistry and Endodontics, Government Dental College and Hospital, Mumbai, Maharashtra, India.
authors: Anand A
authors: AA
authors: Mohatta
authors: Department of Oral Pathology and Microbiology, D.Y Patil Dental College and Hospital, Navi Mumbai, Maharashtra, India.
authors: Jasmine
authors: J
authors: Singh
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D003596:Cytosine; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D013941:Thymine; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D005819:Genetic Markers; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D003596:Cytosine; D013941:Thymine
keywords: oral squamous cell carcinoma; polymerase chain reaction; single nucleotide polymorphism; vascular endothelial growth factor