title: Development of allele-specific multiplex PCR to determine the length of poly-T in intron 8 of CFTR.
abstract: Cystic fibrosis transmembrane conductance regulator (CFTR) gene mutation analysis has been implemented for Cystic Fibrosis (CF) carrier screening, and molecular diagnosis of CF and congenital bilateral absence of the vas deferens (CBAVD). Although poly-T allele analysis in intron 8 of CFTR is required when a patient is positive for R117H, it is not recommended for routine carrier screening. Therefore, commercial kits for CFTR mutation analysis were designed either to mask the poly-T allele results, unless a patient is R117H positive, or to have the poly-T analysis as a standalone reflex test using the same commercial platform. There are other standalone assays developed to detect poly-T alleles, such as heteroduplex analysis, High Resolution Melting (HRM) curve analysis, allele-specific PCR (AS-PCR) and Sanger sequencing. In this report, we developed a simple and easy-to-implement multiplex AS-PCR assay using unlabeled standard length primers, which can be used as a reflex or standalone test for CFTR poly-T track analysis. Out of 115 human gDNA samples tested, results from our new AS-PCR matched to the previous known poly-T results or results from Sanger sequencing.
authors: Department of Pathology and Laboratory Medicine, Beaumont Health System , Royal Oak, MI , USA ; Department of Pathology and Laboratory Medicine, Oakland University William Beaumont School of Medicine , Royal Oak, MI , USA.
authors: Neng
authors: N
authors: Chen
authors: Department of Pathology and Laboratory Medicine, Beaumont Health System , Royal Oak, MI , USA.
authors: Anne E
authors: AE
authors: Prada
keywords: CFTR; Multiplex allele-specific PCR; Poly-T analysis