title: Molecular Characterization and Expression Analysis of Ribosomal Protein S6 Gene in the Cashmere Goat (Capra hircus).
abstract: Ribosomal protein (rp) S6 is the substrate of ribosomal protein S6K (S6 kinase) and is involved in protein synthesis by mTOR/S6K/S6 signaling pathway. Some S6 cDNA have been cloned in mammals in recent years but has not been identified in the goat. To facilitate such studies, we cloned the cDNA encoding Cashmere goat (Capra hircus) S6 (GenBank accession GU131122) and then detected mRNA expression in seven tissues by real time PCR and protein expression in testis tissue by immunohistochemisty. Sequence analysis indicated that the obtained goat S6 was a 808 bp product, including a 3' untranslated region of 58 bp and an open reading frame of 750 bp which predicted a protein of 249 amino acids. The predicted amino acid sequence was highly homologous to cattle, human, mouse and rat S6. Expression analysis indicated S6 mRNA was expressed extensively in detected tissues and S6 protein was expressed in testis tissue.
authors: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors: Bao
authors: B
authors: Wenlei
authors: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors: Hao
authors: H
authors: Xiyan
authors: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors: Zheng
authors: Z
authors: Xu
authors: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors: Liang
authors: L
authors: Yan
authors: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors: Chen
authors: C
authors: Yuhao
authors: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors: Wang
authors: W
authors: Yanfeng
authors: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors: Wang
authors: W
authors: Zhigang
keywords: Cashmere Goat; Expression Pattern; Ribosomal Protein (S6) Gene; The Mammalian Target of Rapamycin (mTOR)