title: Characteristics and behaviour of liposomes when incubated with natural bile salt extract: implications for their use as oral drug delivery systems.
abstract: The use of liposomes for oral administration of drugs and for food applications is based on their ability to preserve entrapped substances and to increase their bioavailability. Bile salts are one of the agents that affect the liposome structure during intestinal digestion and the main reported studies on liposome/bile salt systems used only one bile salt. The aim of this work is to characterise the interaction of liposomes with a natural bile salt extract (BSE) at physiological pH and temperature. Three types of liposomes (fluid, gel-state and liquid-ordered bilayers) were studied. Phase diagrams were obtained and a very different behaviour was found. Fluid bilayers were completely permeable to an entrapped dye with partial or complete disruption of vesicles (final size 10 nm). Gel-state bilayers released their content but BSE led to the formation of large mixed structures (2000 nm). Liquid-ordered bilayers formed mixed vesicles (1000 nm) and, surprisingly, retained a high percentage of their aqueous content (about 50%). As a consequence, each type of liposome offers singular features to be used in oral applications due to their specific interaction with bile salts.
authors: Centre of Research and Development in Chemistry, National Institute of Industrial Technology (INTI), Av. Gral. Paz e/ Constituyentes y Albarellos San Martín, Buenos Aires, Argentina.
authors: Laura G
authors: LG
authors: Hermida
authors: Manuel
authors: M
authors: Sabés-Xamaní
authors: Ramon
authors: R
authors: Barnadas-Rodríguez
mesh_terms: D000284:Administration, Oral; D001647:Bile Acids and Salts / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D002626:Chemistry, Pharmaceutical; D002794:Choline / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008823:Micelles; D008856:Microscopy, Fluorescence; D012995:Solubility; D013696:Temperature
chemical_list: D001647:Bile Acids and Salts; D004337:Drug Carriers; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D008823:Micelles; D002794:Choline