title: MALDI mass spectrometry imaging for the simultaneous location of resveratrol, pterostilbene and viniferins on grapevine leaves.
abstract: To investigate the in-situ response to a stress, grapevine leaves have been subjected to mass spectrometry imaging (MSI) experiments. The Matrix Assisted Laser Desorption/Ionisation (MALDI) approach using different matrices has been evaluated. Among all the tested matrices, the 2,5-dihydroxybenzoic acid (DHB) was found to be the most efficient matrix allowing a broader range of detected stilbene phytoalexins. Resveratrol, but also more toxic compounds against fungi such as pterostilbene and viniferins, were identified and mapped. Their spatial distributions on grapevine leaves irradiated by UV show their specific colocation around the veins. Moreover, MALDI MSI reveals that resveratrol (and piceids) and viniferins are not specifically located on the same area when leaves are infected by Plasmopara viticola. Results obtained by MALDI mass spectrometry imaging demonstrate that this technique would be essential to improve the level of knowledge concerning the role of the stilbene phytoalexins involved in a stress event.
authors: Laboratoire de Chimie et Physique-Approche Multi échelle des Milieux Complexes (LCP-A2MC), Institut Jean Barriol (FR 2843), Université de Lorraine, ICPM 1 Boulevard Arago, F-57078 Metz, France. loic.becker@univ-lorraine.fr.
authors: Loïc
authors: L
authors: Becker
authors: Laboratoire de Chimie et Physique-Approche Multi échelle des Milieux Complexes (LCP-A2MC), Institut Jean Barriol (FR 2843), Université de Lorraine, ICPM 1 Boulevard Arago, F-57078 Metz, France. vincent.carre@univ-lorraine.fr.
authors: Vincent
authors: V
authors: Carré
authors: Institut National de Recherche en Agronomie (INRA) - Santé de la Vigne et Qualité du Vin (UMR 1131), 28 rue de Herrlisheim, F-68021 Colmar, France. anne.poutaraud@colmar.inra.fr.
authors: Anne
authors: A
authors: Poutaraud
authors: Institut National de Recherche en Agronomie (INRA) - Santé de la Vigne et Qualité du Vin (UMR 1131), 28 rue de Herrlisheim, F-68021 Colmar, France. didier.merdinoglu@colmar.inra.fr.
authors: Didier
authors: D
authors: Merdinoglu
authors: Laboratoire de Chimie et Physique-Approche Multi échelle des Milieux Complexes (LCP-A2MC), Institut Jean Barriol (FR 2843), Université de Lorraine, ICPM 1 Boulevard Arago, F-57078 Metz, France. patrick.chaimbault@univ-lorraine.fr.
authors: Patrick
authors: P
authors: Chaimbault
mesh_terms: D009868:Oomycetes / Q000382:microbiology; D010935:Plant Diseases; D018515:Plant Leaves / Q000378:metabolism* / Q000382:microbiology; D000077185:Resveratrol; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013267:Stilbenes / Q000378:metabolism*; D027843:Vitis / Q000378:metabolism* / Q000382:microbiology
chemical_list: D013267:Stilbenes; C107773:pterostilbene; D000077185:Resveratrol