title: Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil.
abstract: Hemoplasmas are bacteria living in feline red blood cells. Feline hemoplasmosis is frequently associated with old male cats that have access to the streets. This study aimed to detect the presence of hemoplasma speciess in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil, using molecular techniques. Between January 2013 and April 2013, EDTA-whole blood samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats). Samples were subjected to PCR assays targeting hemoplasmas 16S rRNA, followed by sequencing, BLAST analysis and phylogenetic analysis. Results show an occurrence of 36.4% for hemoplasmas. Twenty-three cats (15.2%) were positive for 'Candidatus Mycoplasma haemominutum', 17 (11.2%) for M. haemofelis and 15 (9.9%) for 'Candidatus M. turicensis', from PCR. Coinfection by two or three hemoplasmas was found in 25 cats (16.6%). No statistically significant difference between genders or between lifestyles was observed for the presence of hemoplasmas among the cats. Results show different hemoplasma species are present in cat population (Campo Grande, MS, Brazil). It is suggested that a differential diagnosis for feline hemoplasmosis should be made when cats show nonspecific clinical signs of disease with systemic manifestation.
authors: Universidade Estadual Paulista ? UNESP, Jaboticabal, SP, Brazil.
authors: Ana Cláudia Gabriela Alexandre de
authors: AC
authors: Santis
authors: Universidade Católica Dom Bosco ? UCDB, Campo Grande, MS, Brazil.
authors: Heitor Miraglia
authors: HM
authors: Herrera
authors: Universidade Estadual Paulista ? UNESP, Jaboticabal, SP, Brazil.
authors: Keyla Cartens Marques de
authors: KC
authors: Sousa
authors: Universidade Estadual Paulista ? UNESP, Jaboticabal, SP, Brazil.
authors: Luiz Ricardo
authors: LR
authors: Gonçalves
authors: Universidade Estadual Paulista ? UNESP, Jaboticabal, SP, Brazil.
authors: Nathani Cristina Baccarim
authors: NC
authors: Denardi
authors: Centro de Controle de Zoonoses ? CCZ, Campo Grande, MS, Brazil.
authors: Iara Helena
authors: IH
authors: Domingos
authors: Universidade Católica Dom Bosco ? UCDB, Campo Grande, MS, Brazil.
authors: João Bosco Vilela
authors: JB
authors: Campos
authors: Universidade Estadual Paulista ? UNESP, Jaboticabal, SP, Brazil.
authors: Rosangela Zacarias
authors: RZ
authors: Machado
authors: Universidade Estadual Paulista ? UNESP, Jaboticabal, SP, Brazil.
authors: Marcos Rogério
authors: MR
authors: André
mesh_terms: D000818:Animals; D001938:Brazil; D002415:Cats / Q000469:parasitology*; D004912:Erythrocytes / Q000469:parasitology*; D005260:Female; D008297:Male; D025202:Molecular Diagnostic Techniques; D009174:Mycoplasma / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction