title: Multilocus VNTR analysis-ompA typing of venereal isolates of Chlamydia trachomatis in Japan.
abstract: In this study, we investigated the prevalence of genital Chlamydia trachomatis isolated in Japan using a high-resolution genotyping method, the multilocus VNTR analysis (MLVA)-ompA typing method. Seventeen serotypes of C. trachomatis standard strain (A-L3) and 44 clinical isolates were obtained from clinical settings. Genotyping of the ompA gene allowed clinical isolates to be divided into nine serotypes: B (6.8%), D (15.9%), E (25%), F (20.5%), G (18.1%), H (6.8%), Ia (2.3%), J (2.3%), and K (2.3%). These isolates were further divided into 28 types after combining ompA genotyping data with MLVA data (Hunter-Gaston discriminatory index D, 0.949). Thus, our results demonstrated that MLVA could identify clinical isolates that could not be distinguished by ompA typing.
authors: Department of Virology 1, National Institute of Infectious Diseases, Japan.
authors: Masaaki
authors: M
authors: Satoh
authors: Department of Virology 1, National Institute of Infectious Diseases, Japan.
authors: Motohiko
authors: M
authors: Ogawa
authors: Department of Virology 1, National Institute of Infectious Diseases, Japan.
authors: Masayuki
authors: M
authors: Saijo
authors: Department of Virology 1, National Institute of Infectious Diseases, Japan. Electronic address: shuando@nih.go.jp.
authors: Shuji
authors: S
authors: Ando
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan; D008297:Male; D018598:Minisatellite Repeats; D012703:Serotyping
chemical_list: D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins
keywords: Chlamydia trachomatis; Clinical isolates; MLVA-ompA; VNTR