title: Snail-regulated MiR-375 inhibits migration and invasion of gastric cancer cells by targeting JAK2.
abstract: MicroRNAs (miRNAs) have been reported to play a critical role in cancer invasion and metastasis. Our previous study showed that miR-375 frequently downregulated in gastric cancer suppresses cell proliferation by targeting Janus kinase 2 (JAK2). Here, we further found that the expression level of miR-375 is significantly decreased in metastatic gastric cancer tissues compared with the non-metastasis controls. Ectopic expression of miR-375 inhibits the migration and invasion of gastric cancer cells partially by targeting JAK2. Furthermore, miR-375 expression is negatively regulated by the metastasis associated transcription factor Snail, which directly binds to the putative promoter of miR-375. Moreover, overexpression of Snail can partially reverse the inhibition of gastric cancer cell migration caused by miR-375. Taken together, these data suggest that miR-375 may be negatively regulated by Snail and involved in gastric cancer cell migration and invasion potentially by targeting JAK2.
authors: Zhejiang Cancer Hospital, Hangzhou, China; Department of Cell Biology and Program in Molecular cell Biology, Zhejiang University School of Medicine, Hangzhou, China.
authors: Yanjun
authors: Y
authors: Xu
authors: Department of Cell Biology and Program in Molecular cell Biology, Zhejiang University School of Medicine, Hangzhou, China.
authors: Juan
authors: J
authors: Jin
authors: Department of Cell Biology and Program in Molecular cell Biology, Zhejiang University School of Medicine, Hangzhou, China.
authors: Yiman
authors: Y
authors: Liu
authors: Department of Cell Biology and Program in Molecular cell Biology, Zhejiang University School of Medicine, Hangzhou, China.
authors: Zhenxia
authors: Z
authors: Huang
authors: Department of Cell Biology and Program in Molecular cell Biology, Zhejiang University School of Medicine, Hangzhou, China.
authors: Yujie
authors: Y
authors: Deng
authors: Department of Surgery, 2nd Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.
authors: Tao
authors: T
authors: You
authors: Department of Cell Biology and Program in Molecular cell Biology, Zhejiang University School of Medicine, Hangzhou, China; Institute of Gastroenterology, Zhejiang University, Hangzhou, China.
authors: Tianhua
authors: T
authors: Zhou
authors: Institute of Gastroenterology, Zhejiang University, Hangzhou, China; Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Jianmin
authors: J
authors: Si
authors: Department of Cell Biology and Program in Molecular cell Biology, Zhejiang University School of Medicine, Hangzhou, China; Institute of Gastroenterology, Zhejiang University, Hangzhou, China.
authors: Wei
authors: W
authors: Zhuo
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053614:Janus Kinase 2 / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D000071250:Snail Family Transcription Factors; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing / Q000235:genetics
chemical_list: C540933:MIRN375 microRNA, human; D035683:MicroRNAs; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D053614:Janus Kinase 2