title: A streptavidin functionalized graphene oxide/Au nanoparticles composite for the construction of sensitive chemiluminescent immunosensor.
abstract: In this work, a novel streptavidin functionalized graphene oxide/Au nanoparticles (streptavidin/GO/AuNPs) composite is prepared and for the first time used to construct sensitive chemiluminescent immunosensor for the detection of tumor marker. The streptavidin/GO/AuNPs composite and the immunosensor are characterized using scanning electron microscopy, static water contact angle measurement and electrochemical impedance spectroscopy. The biofunctionalized composite has large reactive surface area and excellent biocompatibility, thus the capture antibody can be efficiently immobilized on its surface based on the highly selective recognition of streptavidin to biotinylated antibody. Using α-fetoprotein (AFP) as a model, the proposed chemiluminescent immunosensor shows a wide linear range from 0.001 to 0.1 ng mL(-1) with an extremely low detection limit down to 0.61 pg mL(-1). The resulting AFP immunosensor shows high detection sensitivity, fast assay speed, acceptable detection and fabrication reproducibility, good specificity and stability. The assay results of serum samples with the proposed method are in an acceptable agreement with the reference values. This work provides a promising biofunctionalized nanostructure for sensitive biosensing applications.
authors: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China. Electronic address: zjyang@yzu.edu.cn.
authors: Zhanjun
authors: Z
authors: Yang
authors: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors: Shufen
authors: S
authors: Luo
authors: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors: Juan
authors: J
authors: Li
authors: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors: Juan
authors: J
authors: Shen
authors: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors: Suhua
authors: S
authors: Yu
authors: Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors: Xiaoya
authors: X
authors: Hu
authors: Environmental Engineering and Science Program, School of Energy Environmental, Biological and Medical Engineering, University of Cincinnati, Cincinnati, OH 45221-0012, USA.
authors: Dionysios D
authors: DD
authors: Dionysiou
mesh_terms: D001672:Biocompatible Materials; D015374:Biosensing Techniques; D058266:Dielectric Spectroscopy; D004797:Enzyme-Linked Immunosorbent Assay; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D049449:Luminescence; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D010087:Oxides; D006108:Graphite; D019809:Streptavidin
keywords: Chemiluminescent; Gold nanoparticles; Graphene oxide; Immunosensor; Streptavidin; Tumor marker