title: Expression of Nogo isoforms and Nogo-B receptor (NgBR) in non-small cell lung carcinomas.
abstract: BACKGROUND
  Nogo-B was recently shown to be involved in proliferation, apoptosis and invasiveness of cancer cells, whereas its specific receptor (NgBR) was found to be up-regulated in estrogen receptor-α positive breast cancer. No data are currently available concerning their expression in non-small cell lung carcinomas (NSCLC).
  
  
  METHODS
  Expression of Nogo isoforms and NgBR was studied in 191 NSCLC.
  
  
  RESULTS
  Higher Nogo-A/B immunoreactivity was noted in cancer cells of squamous cell carcinomas (SQC) compared to adenocarcinomas (p<0.001). Stage II-IV tumors had the lowest Nogo-A/B expression (p<0.0001) compared to stage I cases. Nogo-A/B expression decreased with increasing SQC malignancy grade (p=0.026). Significant NgBR mRNA down-regulation was associated with larger primary tumor size (p=0.039), lymph node involvement (p=0.039) and advancement stage (p=0.0054). Low NgBR mRNA expression predicted poor patients outcome (p=0.029).
  
  
  CONCLUSIONS
  The current data may point to the involvement of Nogo isoforms and NgBR in the pathogenesis of NSCLC.
authors: Department of Histology and Embryology, Medical University, Wroclaw, Poland.
authors: Bartosz
authors: B
authors: Pula
authors: Department of Pulmonology and Pulmonary Tumors, Medical University, Wroclaw, Poland.
authors: Bozena
authors: B
authors: Werynska
authors: Department of Histology and Embryology, Medical University, Wroclaw, Poland.
authors: Mateusz
authors: M
authors: Olbromski
authors: Laboratory of Histopathology, Lower Silesia Centre of Pulmonary Diseases, Wroclaw, Poland.
authors: Beata
authors: B
authors: Muszczynska-Bernhard
authors: Surgery Department, 4th Military Teaching Hospital, Wroclaw, Poland.
authors: Mariusz
authors: M
authors: Chabowski
authors: Surgery Department, 4th Military Teaching Hospital, Wroclaw, Poland Department of Clinical Proceedings, Medical University, Wroclaw, Poland.
authors: Dariusz
authors: D
authors: Janczak
authors: Department of Histology and Embryology, Medical University, Wroclaw, Poland Department of Histology and Embryology, Medical University, Poznan, Poland.
authors: Maciej
authors: M
authors: Zabel
authors: Department of Histology and Embryology, Medical University, Wroclaw, Poland.
authors: Marzena
authors: M
authors: Podhorska-Okolow
authors: Department of Histology and Embryology, Medical University, Wroclaw, Poland Department of Physiotherapy, Wroclaw University School of Physical Education, Wroclaw, Poland piotr.dziegiel@umed.wroc.pl.
authors: Piotr
authors: P
authors: Dziegiel
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000209:etiology / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000209:etiology / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009185:Myelin Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D009367:Neoplasm Staging; D000070798:Nogo Proteins; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D009185:Myelin Proteins; C512614:NUS1 protein, human; D000070798:Nogo Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C000603199:RTN4 protein, human; D011956:Receptors, Cell Surface
keywords: Nogo; Nogo-B receptor; Non-small cell lung carcinoma; immunohistochemistry