title: Analyzing gene function in adult long-term hematopoietic stem cells using the interferon inducible Mx1-Cre mouse system.
abstract: Long-term hematopoietic stem cells (LT-HSCs) have the ability to self-renew and differentiate into all blood cell lineages. Understanding the genetic networks that regulate LT-HSC function in the adult bone marrow requires inducible gene targeting and bone marrow transplantations. In this chapter we describe the use of the inducible Mx1-Cre mouse model to delete genes in LT-HSCs and methodologies for examining the function of LT-HSCs following deletion.
authors: Department of Pediatrics, Uniformed Services University of the Health Sciences, Bridge Road, Bethesda, MD, 20814, USA, kristbjorn.gudmundsson.ctr@usuhs.edu.
authors: Kristbjorn Orri
authors: KO
authors: Gudmundsson
authors: Kevin
authors: K
authors: Oakley
authors: Yufen
authors: Y
authors: Han
authors: Yang
authors: Y
authors: Du
mesh_terms: D053687:Adult Stem Cells / Q000378:metabolism*; D000818:Animals; D016026:Bone Marrow Transplantation; D005434:Flow Cytometry; D018390:Gene Targeting / Q000379:methods*; D060005:Genotyping Techniques; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D007267:Injections; D019426:Integrases / Q000378:metabolism*; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D016297:Mutagenesis, Site-Directed / Q000379:methods; D064547:Myxovirus Resistance Proteins / Q000235:genetics*; D011066:Poly C / Q000494:pharmacology
chemical_list: C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins; D011066:Poly C; D007372:Interferons; C045073:Cre recombinase; D019426:Integrases