title: Characterization of amniotic stem cells.
abstract: The amnion membrane is developed from embryo-derived cells, and amniotic cells have been shown to exhibit multidifferentiation potential. These cells represent a desirable source for stem cells for a variety of reasons. However, to date very few molecular analyses of amnion-derived cells have been reported, and efficient markers for isolating the stem cells remain unclear. This paper assesses the characterization of amnion-derived cells as stem cells by examining stemness marker expressions for amnion-derived epithelial cells and mesenchymal cells by flow cytometry, immunocytochemistry, and quantitative PCR. Flow cytometry revealed that amnion epithelial cells expressed CD133, CD 271, and TRA-1-60, whereas mecenchymal cells expressed CD44, CD73, CD90, and CD105. Immunohistochemistry showed that both cells expressed the stemness markers Oct3/4, Sox2, Klf4, and SSEA4. Stemness genes' expression in amnion epithelial cells, mesenchymal cells, fibroblast, bone marrow-derived mesenchymal stem cells (MSCs), and induced pluripotent stem cells (iPSCs) was compared by quantitative reverse-transcription polymerase chain reaction (RT-PCR). Amnion-derived epithelial cells and mesenchymal cells expressed Oct3/4, Nanog, and Klf4 more than bone marrow-derived MSCs. The sorted TRA1-60-positive cells expressed Oct3/4, Nanog, and Klf4 more than unsorted cells or TRA1-60-negative cells. TRA1-60 can be a marker for isolating amnion epithelial stem cells.
authors: 1 Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama , Toyama, 930-0194, Japan .
authors: Chika
authors: C
authors: Koike
authors: Kaixuan
authors: K
authors: Zhou
authors: Yuji
authors: Y
authors: Takeda
authors: Moustafa
authors: M
authors: Fathy
authors: Motonori
authors: M
authors: Okabe
authors: Toshiko
authors: T
authors: Yoshida
authors: Yukio
authors: Y
authors: Nakamura
authors: Yukio
authors: Y
authors: Kato
authors: Toshio
authors: T
authors: Nikaido
mesh_terms: D000650:Amnion / Q000166:cytology / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D000090062:Kruppel-Like Factor 4; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D000943:Antigens, Differentiation; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4