title: [Amentoflavone induces apoptosis in SW480 human colorectal cancer cells via regulating β-catenin and caspase-3 expressions].
abstract: OBJECTIVE
  To investigate the role of β-catenin and caspase-3 in amentoflavone-induced apoptosis of human colorectal cancer SW480 cells.
  
  
  METHODS
  MTT assay was used to detect the viability of SW480 cells exposed to amentoflavone, and flow cytometry was employed to assess the cell apoptosis. Western blotting was performed to determine the protein expressions of β-catenin and caspase-3 in the exposed cells.
  
  
  RESULTS
  Amentoflavone dose-dependently inhibited the viability of SW480 cells, and a high concentration of amentoflavone (150 µmol/L) obviously induced apoptosis of the cells. Amentoflavone exposure caused significantly increased expression of caspase-3 and suppressed β-catenin expression in the cells.
  
  
  CONCLUSIONS
  Amentoflavone-induced apoptosis in SW480 human colorectal cancer cells is associated with altered expressions of β-catenin and caspase-3.
authors: Laboratory of Molecular Biology, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. E-mail: xiaopilv2001@163.com.
authors: Yu
authors: Y
authors: Yang
authors: Wenjuan
authors: W
authors: Xu
authors: Kang
authors: K
authors: Peng
authors: Xuegang
authors: X
authors: Sun
mesh_terms: D017209:Apoptosis; D044946:Biflavonoids / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism*; D045744:Cell Line, Tumor / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000473:pathology*; D006801:Humans; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D044946:Biflavonoids; C495270:CTNNB1 protein, human; D051176:beta Catenin; C011164:amentoflavone; C505407:CASP3 protein, human; D053148:Caspase 3