title: Synthetic induction of immunogenic cell death by genetic stimulation of endoplasmic reticulum stress.
abstract: Cis-diamminedichloridoplatinum(II) (CDDP), commonly referred to as cisplatin, is a chemotherapeutic drug used for the treatment of a wide range of solid cancers. CDDP is a relatively poor inducer of immunogenic cell death (ICD), a cell death modality that converts dying cells into a tumor vaccine, stimulating an immune response against residual cancer cells that permits long-lasting immunity and a corresponding reduction in tumor growth. The incapacity of CDDP to trigger ICD is at least partially due to its failure to stimulate the premortem endoplasmic reticulum (ER)-stress response required for the externalization of the "eat-me" signal calreticulin (CRT) on the surface of dying cancer cells. Here, we developed a murine cancer cell line genetically modified to express the ER resident protein reticulon-1c (Rtn-1c) by virtue of tetracycline induction and showed that enforced Rtn-1c expression combined with CDDP treatment promoted CRT externalization to the surface of cancer cells. In contrast to single agent treatments, the tetracycline-mediated Rtn-1c induction combined with CDDP chemotherapy stimulated ICD as measured by the capacity of dying tumor cells, inoculated into syngenic immunocompetent mice, to mount an immune response to tumor re-challenge 1 week later. More importantly, established tumors, forced to constitutively express Rtn-1c in vivo by continuous treatment with tetracycline, became responsive to CDDP and exhibited a corresponding reduction in the rate of tumor growth. The combined therapeutic effects of Rtn-1c induction with CDDP treatment was only detected in the context of an intact immune system and not innu/numice lacking thymus-dependent T lymphocytes. Altogether, these results indicate that the artificial or "synthetic" induction of immunogenic cell death by genetic manipulation of the ER-stress response can improve the efficacy of chemotherapy with CDDP by stimulating anticancer immunity.
authors: Equipe 11 labellisée par la Ligue Nationale contre le cancer; INSERM U1138; Centre de Recherche des Cordeliers; Paris, France ; Metabolomics and Molecular Cell Biology Platforms; Gustave Roussy Comprehensive Cancer Center; Villejuif, France ; Université Paris Descartes/Paris V; Sorbonne Paris Cité; Paris, France.
authors: Mickaël
authors: M
authors: Michaud
authors: Equipe 11 labellisée par la Ligue Nationale contre le cancer; INSERM U1138; Centre de Recherche des Cordeliers; Paris, France ; Metabolomics and Molecular Cell Biology Platforms; Gustave Roussy Comprehensive Cancer Center; Villejuif, France ; Université Paris Descartes/Paris V; Sorbonne Paris Cité; Paris, France.
authors: Abdul Qader
authors: AQ
authors: Sukkurwala
authors: Department of Biology; University of Rome 'Tor Vergata'; Rome, Italy.
authors: Federica
authors: F
authors: Di Sano
authors: INSERM U1015; Villejuif, France ; Université Paris Sud-XI; Faculté de Médecine; Le Kremlin Bicêtre, France ; Center of Clinical Investigations in Biotherapies of Cancer (CICBT) 507; Villejuif, France.
authors: Laurence
authors: L
authors: Zitvogel
authors: Equipe 11 labellisée par la Ligue Nationale contre le cancer; INSERM U1138; Centre de Recherche des Cordeliers; Paris, France ; Metabolomics and Molecular Cell Biology Platforms; Gustave Roussy Comprehensive Cancer Center; Villejuif, France ; Université Paris Descartes/Paris V; Sorbonne Paris Cité; Paris, France.
authors: Oliver
authors: O
authors: Kepp
authors: Equipe 11 labellisée par la Ligue Nationale contre le cancer; INSERM U1138; Centre de Recherche des Cordeliers; Paris, France ; Metabolomics and Molecular Cell Biology Platforms; Gustave Roussy Comprehensive Cancer Center; Villejuif, France ; Université Paris Descartes/Paris V; Sorbonne Paris Cité; Paris, France ; Pôle de Biologie; Hôpital Européen Georges Pompidou; AP-HP; Paris, France.
authors: Guido
authors: G
authors: Kroemer
keywords: calreticulin; cancer; chemotherapy; cisplatin; endoplasmic reticulum stress; reticulon 1C