title: Cerebrospinal fluid can be used for HIV genotyping when it fails in blood.
abstract: Blood plasma specimens are the clinical standard for HIV-1 pol gene genotyping from viral populations; however, it is not always successful, often from low viral loads or the presence of polymerase chain reaction (PCR) inhibitors. Objective To describe the successful of HIV-1 genotyping in two samples of cerebrospinal fluid (CSF), after genotype procedures failed from blood. Method Two HIV-infected patients enrolled in a neurocognitive research study were evaluated when standard HIV-1 genotyping failed from blood plasma samples. Genotyping was performed using the commercial system TRUGENE HIV-1 Genotyping Kit and the OpenGene DNA Sequencing System (Siemens Healthcare Diagnostics, Tarrytown, NY, USA). Results CSF genotyping was performed via the same commercial platform and was successful in both cases. Conclusion This report demonstrates that CSF could be used as an alternate clinical specimen for HIV-1 genotyping when it fails from blood.
authors: Laboratório de Virologia, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors: Indianara
authors: I
authors: Rotta
authors: Serviço de Infectologia, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors: Sonia Mara
authors: SM
authors: Raboni
authors: Serviço de Infectologia, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors: Cléa Elisa Lopes
authors: CE
authors: Ribeiro
authors: Laboratório da Secretaria Municipal de Saúde, Curitiba, PR, Brazil.
authors: Maristela
authors: M
authors: Riedel
authors: Laboratório da Secretaria Municipal de Saúde, Curitiba, PR, Brazil.
authors: Maria da Graça
authors: Mda G
authors: Winhescki
authors: University of California, San Diego, CA, USA.
authors: Davey M
authors: DM
authors: Smith
authors: University of California, San Diego, CA, USA.
authors: Ronald J
authors: RJ
authors: Ellis
authors: Laboratório de Virologia, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors: Sérgio Monteiro
authors: SM
authors: de Almeida
mesh_terms: D000328:Adult; D001483:Base Sequence; D060005:Genotyping Techniques / Q000379:methods*; D015658:HIV Infections / Q000097:blood / Q000134:cerebrospinal fluid*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification; D015203:Reproducibility of Results; D019562:Viral Load
chemical_list: D012367:RNA, Viral