title: Evaluation of seed extracts from plants found in the Caatinga biome for the control of Aedes aegypti.
abstract: Dengue fever, currently the most important arbovirus, is transmitted by the bite of the Aedes aegypti mosquito. Given the absence of a prophylactic vaccine, the disease can only be controlled by combating the vector insect. However, increasing reports of resistance and environmental damage caused by insecticides have led to the urgent search for new safer alternatives. In this regard, plants stand out as a source of easy-to-obtain biodegradable insecticide molecules. Twenty (20) plant seed extracts from the Caatinga, an exclusively Brazilian biome, were prepared. Sodium phosphate (50 mM, pH 8.0) was used as extractor. The extracts were used in bioassays and submitted to partial characterisation. A Probit analysis of insecticides was carried out, and intergroup differences were verified by the Student's t test and ANOVA. All the extracts exhibited larvicidal and ovipositional deterrence activity. The extracts of Amburana cearenses, Piptadenia viridiflora, Erythrina velutina, Myracrodruon urundeuva and Schinopsis brasiliensis were also pupicides, while the extracts of P. viridiflora, E. velutina, A. cearenses, Anadenanthera colubrina, Diocleia grandiflora, Bauhinia cheilantha, Senna spectabilis, Caesalpinia pyramidalis, Mimosa regnelli and Genipa americana displayed adulticidal activity. Egg laying was compromised when females were fed extracts of Ricinus communis, Croton sonderianus and S. brasiliensis. At least two proteins with insecticidal activity were found in all the extracts. Phenol compounds were identified in all the extracts and flavonoids, triterpenes or alkaloids in 14 of them. The results show the potential of plant seed extracts from the Caatinga as a source of active molecules against A. aegypti mosquitos.
authors: Postgraduate Biochemistry Program, Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), 3000 Avenida Senador Salgado Filho Ave, Lagoa Nova, Natal, RN, 59078 970, Brazil.
authors: Patrícia Batista Barra Medeiros
authors: PB
authors: Barbosa
authors: Julliete Medeiros
authors: JM
authors: de Oliveira
authors: Juliana Macêdo
authors: JM
authors: Chagas
authors: Luciana Maria Araujo
authors: LM
authors: Rabelo
authors: Guilherme Fulgêncio
authors: GF
authors: de Medeiros
authors: Raquel Brant
authors: RB
authors: Giodani
authors: Elizeu Antunes
authors: EA
authors: da Silva
authors: Adriana Ferreira
authors: AF
authors: Uchôa
authors: Maria
authors: M
authors: de Fátima de Freire Melo Ximenes
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000470:Alkaloids / Q000032:analysis; D027926:Anacardiaceae / Q000737:chemistry; D000818:Animals; D001938:Brazil; D029911:Caesalpinia / Q000737:chemistry; D002470:Cell Survival; D017753:Ecosystem; D004895:Erythrina / Q000737:chemistry; D007887:Fabaceae / Q000737:chemistry; D005260:Female; D005419:Flavonoids / Q000032:analysis; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D007814:Larva; D008297:Male; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis
chemical_list: D000470:Alkaloids; D005419:Flavonoids; D007306:Insecticides; D010636:Phenols; D010936:Plant Extracts; D014315:Triterpenes