title: Alterations of endogenous metabolites in urine of rats exposed to decabromodiphenyl ether using metabonomic approaches.
abstract: There is large usage of polybrominated diphenyl ethers (PBDEs) especially for decabromodiphenyl ether (BDE-209, Deca-BDE) in controlling the risks of fire. The toxicological effects of PBDEs are worth being concerned about. Female SD rats were daily gavaged with BDE-209 ether at the dose of 100 mg/kg for 20 days. Histological observation was performed for the screening of the target organs for BDE-209 exposure. The distribution and metabolism of PBDEs in the exposed main organs were evidenced by HRGC-HRMS. Alterations of the endogenous metabolite concentrations in urine were investigated using metabonomic approaches based on (1)H NMR spectrum. Histopathological changes including serious edema in kidney, hepatocellular spotty necrosis and perivasculitis in liver indicated that BDE-209 caused potential influences on endogenous metabolism in the exposed liver and the kidney. BDE-209 was found to be highly accumulated in lipid, ovary, kidney and liver after 20 days' exposure. Occurrence of other lower brominated PBDEs in the rats demonstrated that reductive debromination process happened in vivo. Hydroxylated and methoxylated-BDEs, as metabolism products, were also detected in the rat tissues. A total of 12 different endogenous metabolites showed obvious alterations in urine from the exposed rats, indicating the disturbance of the corresponding internal biochemical processes induced by BDE-209 exposure. These findings in vivo suggested the potential health risk might be of concern due to the toxicological effects of BDE-209 as a ubiquitous compound in the environment.
authors: State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: wjyang@cashq.ac.cn.
authors: Weijin
authors: W
authors: Yang
authors: State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors: Jianjie
authors: J
authors: Fu
authors: State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors: Thanh
authors: T
authors: Wang
authors: State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors: Hanxia
authors: H
authors: Liu
authors: State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors: Yawei
authors: Y
authors: Wang
authors: State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: zhouqf@rcees.ac.cn.
authors: Qunfang
authors: Q
authors: Zhou
authors: State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors: Guibin
authors: G
authors: Jiang
mesh_terms: D000818:Animals; D005260:Female; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity / Q000652:urine*; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D055768:Halogenated Diphenyl Ethers; C010902:decabromobiphenyl ether
keywords: decabromodiphenyl ether; endogenous metabolites; metabonomic approaches