title: Genomic characterization and transcriptional studies of the starch-utilizing strain Bifidobacterium adolescentis 22L.
abstract: Bifidobacteria are members of the gut microbiota, but the genetic basis for their adaptation to the human gut is poorly understood. The analysis of the 2,203,222-bp genome of Bifidobacterium adolescentis 22L revealed a nutrient acquisition strategy that targets diet/plant-derived glycans, in particular starch and starch-like carbohydrates. Starch-like carbohydrates were shown to support the growth of B. adolescentis 22L. Transcriptome profiling of 22L cultures grown under in vitro conditions or during colonization of the murine gut by RNA sequencing and quantitative real-time PCR assays revealed the expression of a set of chromosomal loci responsible for starch metabolism as well as for pilus production. Such extracellular structures include so-called sortase-dependent and type IVb pili, which may be involved in gut colonization of 22L through adhesion to extracellular matrix proteins.
authors: Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy.
authors: Sabrina
authors: S
authors: Duranti
authors: Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Cork, Ireland.
authors: Francesca
authors: F
authors: Turroni
authors: Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy.
authors: Gabriele Andrea
authors: GA
authors: Lugli
authors: Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy.
authors: Christian
authors: C
authors: Milani
authors: GenProbio Ltd., Parma, Italy.
authors: Alice
authors: A
authors: Viappiani
authors: GenProbio Ltd., Parma, Italy.
authors: Marta
authors: M
authors: Mangifesta
authors: Department of Neurosciences, University of Parma, Parma, Italy.
authors: Laura
authors: L
authors: Gioiosa
authors: Department of Neurosciences, University of Parma, Parma, Italy.
authors: Paola
authors: P
authors: Palanza
authors: Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Cork, Ireland.
authors: Douwe
authors: D
authors: van Sinderen
authors: Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy marco.ventura@unipr.it.
authors: Marco
authors: M
authors: Ventura
mesh_terms: D000818:Animals; D001483:Base Sequence; D039941:Bifidobacteriales Infections / Q000382:microbiology*; D001644:Bifidobacterium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005260:Female; D010861:Fimbriae, Bacterial / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology; D020869:Gene Expression Profiling; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013213:Starch / Q000378:metabolism*
chemical_list: D013213:Starch