title: Halopenitus salinus sp. nov., isolated from the brine of salted brown alga Laminaria.
abstract: A halophilic archaeal strain, SKJ47(T), was isolated from a commercial preparation of the brown alga Laminaria produced at Dalian, Liaoning Province, China. Cells of the strain were observed to be short rods, stain Gram-negative, and to form red-pigmented colonies on solid media. Strain SKJ47(T) was found to be able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 2.6-3.1 M), at pH 6.0-9.5 (optimum pH 7.0). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell-lysis was found to be 5% (w/v). The major polar lipids of the strain were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and two glycolipids chromatographically identical to those of Halopenitus persicus IBRC 10041(T). The 16S rRNA gene and rpoB' gene of strain SKJ47(T) were found to be phylogenetically related to the corresponding genes of Halopenitus malekzadehii IBRC-M 10418(T) (96.3 and 91.9% nucleotide identity, respectively) and Hpt. persicus IBRC 10041(T) (96.2 and 93.8%). The DNA G+C content of strain SKJ47(T) was determined to be 65.0 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain SKJ47(T) (=CGMCC 1.12229(T) = JCM 18641(T)) represents a new species of the genus Halopenitus, for which the name Halopenitus salinus sp. nov. is proposed.
authors: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
authors: Dong
authors: D
authors: Han
authors: Heng-Lin
authors: HL
authors: Cui
authors: Zheng-Rong
authors: ZR
authors: Li
mesh_terms: D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D019605:Euryarchaeota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007795:Laminaria / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D006017:Glycolipids; D010743:Phospholipids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D012492:Salts; C017082:brine; D012965:Sodium Chloride; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit