title: Threonine-4 of the budding yeast RNAP II CTD couples transcription with Htz1-mediated chromatin remodeling.
abstract: The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAP II) consists of repeated YSPTSPS heptapeptides and connects transcription with cotranscriptional events. Threonine-4 (Thr4) of the CTD repeats has been shown to function in histone mRNA 3'-end processing in chicken cells and in transcriptional elongation in human cells. Here, we demonstrate that, in budding yeast, Thr4, although dispensable for growth in rich media, is essential in phosphate-depleted or galactose-containing media. Thr4 is required to maintain repression of phosphate-regulated (PHO) genes under normal growth conditions and for full induction of PHO5 and the galactose-induced GAL1 and GAL7 genes. We identify genetic links between Thr4 and the histone variant Htz1 and show that Thr4, as well as the Ino80 chromatin remodeler, is required for activation-associated eviction of Htz1 specifically from promoters of the Thr4-dependent genes. Our study uncovers a connection between transcription and chromatin remodeling linked by Thr4 of the CTD.
authors: Department of Biological Sciences, Columbia University, New York, NY 10027; and.
authors: Emanuel
authors: E
authors: Rosonina
authors: Department of Biological Sciences, Columbia University, New York, NY 10027; and.
authors: Nathan
authors: N
authors: Yurko
authors: Department of Biochemistry and Molecular Biology, Rutgers New Jersey Medical School, Newark, NJ 07101.
authors: Wencheng
authors: W
authors: Li
authors: Department of Biochemistry and Molecular Biology, Rutgers New Jersey Medical School, Newark, NJ 07101.
authors: Mainul
authors: M
authors: Hoque
authors: Department of Biochemistry and Molecular Biology, Rutgers New Jersey Medical School, Newark, NJ 07101.
authors: Bin
authors: B
authors: Tian
authors: Department of Biological Sciences, Columbia University, New York, NY 10027; and jlm2@columbia.edu.
authors: James L
authors: JL
authors: Manley
mesh_terms: D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D003470:Culture Media; D005800:Genes, Fungal; D006657:Histones / Q000502:physiology*; D010766:Phosphorylation; D012319:RNA Polymerase II / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D013912:Threonine / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D003470:Culture Media; D006657:Histones; C493530:Htz1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013912:Threonine; D012319:RNA Polymerase II
keywords: H2A.Z; SWRc