title: miR-10a regulates proliferation of human cardiomyocyte progenitor cells by targeting GATA6.
abstract: microRNAs (miRNAs) play essential roles in cardiogenesis. The altered expression of miRNAs can result in cardiac malformations by inducing abnormalities in the behavior of cardiac cells. However, the role of miR-10a in the regulation of cardiomyocyte progenitor cells (CMPCs) remains undetermined. In the present study, we found that up- or down-regulation of miR-10a inhibited or promoted the proliferation of human CMPCs, respectively, without affecting their differentiation toward cardiomyocytes. miR-10a bound to GATA6 directly and reduced GATA6 expression. Over-expression of GATA6 greatly attenuated the miR-10a-mediated inhibitory effect on the proliferation of human CMPCs. Thus, our results indicate that miR-10a could effectively modulate the proliferation of human CMPCs by targeting GATA6. The finding provides novel insights into the potency of miR-10a during heart development.
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China.
authors: Dandan
authors: D
authors: Liang
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China; Department of Cardiology, East Hospital, Tongji University, Shanghai, China.
authors: Lixiao
authors: L
authors: Zhen
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China.
authors: Tianyou
authors: T
authors: Yuan
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China; Department of Cardiology, East Hospital, Tongji University, Shanghai, China.
authors: Jian
authors: J
authors: Huang
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China; Department of Cardiology, East Hospital, Tongji University, Shanghai, China.
authors: Fangfei
authors: F
authors: Deng
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China; Department of Cardiology, East Hospital, Tongji University, Shanghai, China.
authors: Wuyahan
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China.
authors: Hong
authors: H
authors: Zhang
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China; Department of Cardiology, East Hospital, Tongji University, Shanghai, China.
authors: Lei
authors: L
authors: Pan
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China.
authors: Yi
authors: Y
authors: Liu
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China; Department of Cardiology, East Hospital, Tongji University, Shanghai, China.
authors: Erlinda
authors: E
authors: The
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China.
authors: Zuoren
authors: Z
authors: Yu
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China; Department of Pathology and Pathophysiology, Tongji University School of Medicine, Shanghai, China.
authors: Weidong
authors: W
authors: Zhu
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China.
authors: Yuzhen
authors: Y
authors: Zhang
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China; Department of Pathology and Pathophysiology, Tongji University School of Medicine, Shanghai, China.
authors: Li
authors: L
authors: Li
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China; Department of Pathology and Pathophysiology, Tongji University School of Medicine, Shanghai, China.
authors: Luying
authors: L
authors: Peng
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China.
authors: Jun
authors: J
authors: Li
authors: Key Laboratory of Arrhythmia of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, China; Institute of Medical Genetics, Tongji University, Shanghai, China; Department of Cardiology, East Hospital, Tongji University, Shanghai, China; Department of Pathology and Pathophysiology, Tongji University School of Medicine, Shanghai, China.
authors: Yi-Han
authors: YH
authors: Chen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D050993:GATA6 Transcription Factor / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D032386:Myoblasts, Cardiac / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*
chemical_list: D050993:GATA6 Transcription Factor; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger