title: Design of ratiometric emission probe with visible light excitation for determination of Ca2+ in living cells.
abstract: An organic salt as a fluorescent probe based on intramolecular charge transfer for Ca(2+) determination is developed. Ca(2+) can be detected by ratiometric emission at 490 and 594 nm with an excitation wavelength of 405 nm. This probe is highly selective for Ca(2+) over other divalent metal cations and displays a large Stokes shift of 189 nm that can avoid interference of the excitation light beam and autofluorescence of biological samples. The dissociation constant for Ca(2+) is 2.25 ± 0.47 μM and pertinent to Ca(2+) detection in cellular resting and dynamic states. The probe demonstrates its application in monitoring Ca(2+) in living cells under confocal microscopic imaging.
authors: Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University , Taiyuan 030006, China.
authors: Qiaoling
authors: Q
authors: Liu
authors: Huizhi
authors: H
authors: Du
authors: Xiaoze
authors: X
authors: Ren
authors: Wei
authors: W
authors: Bian
authors: Li
authors: L
authors: Fan
authors: Shaomin
authors: S
authors: Shuang
authors: Chuan
authors: C
authors: Dong
authors: Qin
authors: Q
authors: Hu
authors: Martin M F
authors: MM
authors: Choi
mesh_terms: D002118:Calcium / Q000032:analysis*; D002413:Cations, Divalent / Q000032:analysis; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008027:Light; D018613:Microscopy, Confocal; D061848:Optical Imaging; D010069:Oxadiazoles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D010069:Oxadiazoles; C583463:1,3,4-oxadiazole; D004533:Egtazic Acid; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium