title: Molecular screening of carbapenemase-producing Gram-negative strains in Romanian intensive care units during a one year survey.
abstract: This is the first study, to our knowledge, performed on a significant number of strains (79 carbapenem-resistant Enterobacteriaceae and 84 carbapenem-resistant non-fermenting Gram-negative rods, GNRs) isolated from tissue samples taken from patients in the intensive care units of two large hospitals in Bucharest, Romania, between 2011 and 2012. The results revealed a high prevalence and great diversity of carbapenemase genes (CRG), in both fermenting and non-fermenting Gram-negative carbapenem-resistant strains. The molecular screening of carbapenem-resistant GNRs revealed the presence of worldwide-distributed CRGs (i.e. blaOXA-48 and blaNDM-1 in Enterobacteriaceae and blaOXA-23, blaVIM-4, blaOXA-10-like, blaOXA-60-like, blaSPM-like and blaGES-like in non-fermenting GNRs), reflecting the rapid evolution and spread of carbapenemase producers, particularly in hospitals. Rapid identification of the colonized or infected patients is required, as are epidemiological investigations to establish the local or imported origin of the respective strains.
authors: Department of Microbiology, Faculty of Biology, University of Bucharest, Portocalelor 1-3, Bucharest, Romania.
authors: Irina
authors: I
authors: Gheorghe
authors: Department of Microbiology, Faculty of Biology, University of Bucharest, Portocalelor 1-3, Bucharest, Romania.
authors: Ilda
authors: I
authors: Czobor
authors: Cantacuzino National Institute of Research and Development for Microbiology and Immunology, Splaiul Independenţei 103, Bucharest, Romania.|Research Institute of the University of Bucharest, University of Bucharest, B.P. Hasdeu Street 10, Bucharest, Romania.|Department of Microbiology, Faculty of Biology, University of Bucharest, Portocalelor 1-3, Bucharest, Romania.
authors: Mariana Carmen
authors: MC
authors: Chifiriuc
authors: Fundeni Clinical Hospital, Fundeni Road 258, Bucharest, Romania.
authors: Elvira
authors: E
authors: Borcan
authors: Fundeni Clinical Hospital, Fundeni Road 258, Bucharest, Romania.
authors: Camelia
authors: C
authors: Ghiţă
authors: C. C. Iliescu Institute of Cardiovascular Diseases, Fundeni Road 258, Bucharest.
authors: Otilia
authors: O
authors: Banu
authors: Department of Microbiology, Faculty of Biology, University of Bucharest, Portocalelor 1-3, Bucharest, Romania.
authors: Veronica
authors: V
authors: Lazăr
authors: Department of Microbiology, Faculty of Biology, University of Bucharest, Portocalelor 1-3, Bucharest, Romania.
authors: Grigore
authors: G
authors: Mihăescu
authors: Department of Anatomy and Biophysics, Faculty of Biology, University of Bucharest, Splaiul Independenţei 91-95, Bucharest, Romania.
authors: Dan Florin
authors: DF
authors: Mihăilescu
authors: Centre of Infectious Diseases, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu, China.
authors: Zong
authors: Z
authors: Zhiyong
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D003625:Data Collection; D005838:Genotype; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D007362:Intensive Care Units; D012383:Romania; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase