title: Plasma proteomic changes during hypothermic and normothermic cardiopulmonary bypass in aortic surgeries.
abstract: Deep hypothermic circulatory arrest (DHCA) is a protective method against brain ischemia in aortic surgery. However, the possible effects of DHCA on the plasma proteins remain to be determined. In the present study, we used novel high‑throughput technology to compare the plasma proteomes during DHCA (22˚C) with selective cerebral perfusion (SCP, n=7) to those during normothermic cardiopulmonary bypass (CPB, n=7). Three plasma samples per patient were obtained during CPB: T1, prior to cooling; T2, during hypothermia; T3, after rewarming for the DHCA group and three corresponding points for the normothermic group. A proteomic analysis was performed using isobaric tag for relative and absolute quantification (iTRAQ) labeling tandem mass spectrometry to assess quantitative protein changes. In total, the analysis identified 262 proteins. The bioinformatics analysis revealed a significant upregulation of complement activation at T2 in normothermic CPB, which was suppressed in DHCA. These findings were confirmed by the changes of the terminal complement complex (SC5b‑9) levels. At T3, however, the level of SC5b‑9 showed a greater increase in DHCA compared to normothermic CPB, while 48 proteins were significantly downregulated in DHCA. The results demonstrated that DHCA and rewarming potentially exert a significant effect on the plasma proteome in patients undergoing aortic surgery.
authors: Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Shimane University Faculty of Medicine, Shimane, Japan.
authors: Teiji
authors: T
authors: Oda
authors: Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Shimane University Faculty of Medicine, Shimane, Japan.
authors: Akane
authors: A
authors: Yamaguchi
authors: Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Shimane University Faculty of Medicine, Shimane, Japan.
authors: Masao
authors: M
authors: Yokoyama
authors: Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Shimane University Faculty of Medicine, Shimane, Japan.
authors: Koji
authors: K
authors: Shimizu
authors: Department of Anesthesiology, Shimane University Faculty of Medicine, Shimane, Japan.
authors: Kosaku
authors: K
authors: Toyota
authors: Department of Anesthesiology, Shimane University Faculty of Medicine, Shimane, Japan.
authors: Tetsuro
authors: T
authors: Nikai
authors: Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Shimane, Japan.
authors: Ken-Ichi
authors: K
authors: Matsumoto
mesh_terms: D000368:Aged; D001011:Aorta / Q000378:metabolism* / Q000601:surgery*; D001784:Blood Gas Analysis; D001798:Blood Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002256:Carbonic Anhydrases / Q000378:metabolism; D002315:Cardiopulmonary Bypass; D019295:Computational Biology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D063990:Gene Ontology; D006801:Humans; D007036:Hypothermia, Induced; D007430:Intraoperative Care; D007553:Isotope Labeling; D008297:Male; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D001798:Blood Proteins; D020543:Proteome; D002256:Carbonic Anhydrases