title: Effects of triclosan on the detoxification system in the yellow catfish (Pelteobagrus fulvidraco): expressions of CYP and GST genes and corresponding enzyme activity in phase I, II and antioxidant system.
abstract: Triclosan (TCS), a broad-spectrum antibacterial agent widely used in pharmaceuticals and personal case products (PPCPs), has been universally detected in aquatic ecosystem in recent years. Unfortunately, there is limited information about its potential impacts on responses of genes and enzymes related to fish detoxification. In the present work, we cloned CYP3A and alpha-GST of yellow catfish (Pelteobagrus fulvidraco) and tested the transcriptional expression of CYP1A, CYP3A and GST as well as the alterations of their corresponding enzymes, including ethoxyresorufin-O-deethylase (EROD), aminopyrine N-demethylase (APND), erythromycin N-demethylase (ERND), glutathione S-transferase (GST) and catalase (CAT), and also the oxidative product malondialdehyde (MDA) content in the liver of P. fulvidraco exposed to TCS. Amino acids of CYP3A and GST were deduced and phylogenetic tree was constructed respectively. High identity percent was exhibited between P. fulvidraco and other species, such as other fish, birds and mammals. Results indicated that TCS significantly elevated CYP1A and GST but decreased CYP3A expression, EROD activity and MDA content at lower concentrations of TCS at 24h. Moreover, CYP3A and GST were significantly inhibited at 72 h but induced at 168 h at lower concentrations. However, CYP3A was always induced at the highest concentration during the exposure period. Furthermore, CYP3A, GST, GST enzyme and MDA content exhibited a dose-effect relationship to some extent, but no significant responses were observed in ERND, APND and CAT except for individual treatments. Taken together, EROD was the most sensitive to TCS exposure as compared to other enzymes. Meanwhile, mRNA responses were more sensitive in yellow catfish.
authors: Department of Ecology/Hydrobiology Research Institute, Jinan University, Guangzhou 510632, China.
authors: Peijia
authors: P
authors: Ku
authors: Department of Ecology/Hydrobiology Research Institute, Jinan University, Guangzhou 510632, China.
authors: Xiaoyan
authors: X
authors: Wu
authors: Department of Ecology/Hydrobiology Research Institute, Jinan University, Guangzhou 510632, China; Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China. Electronic address: txpnie@jnu.edu.cn.
authors: Xiangping
authors: X
authors: Nie
authors: Department of Ecology/Hydrobiology Research Institute, Jinan University, Guangzhou 510632, China.
authors: Ruikang
authors: R
authors: Ou
authors: Department of Ecology/Hydrobiology Research Institute, Jinan University, Guangzhou 510632, China.
authors: Lan
authors: L
authors: Wang
authors: Department of Ecology/Hydrobiology Research Institute, Jinan University, Guangzhou 510632, China.
authors: Tian
authors: T
authors: Su
authors: Department of Ecology/Hydrobiology Research Institute, Jinan University, Guangzhou 510632, China.
authors: Yigang
authors: Y
authors: Li
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000633:toxicity*; D002397:Catfishes / Q000378:metabolism*; D002681:China; D019363:Cytochrome P-450 CYP1A1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008658:Inactivation, Metabolic / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D050216:Metabolic Detoxication, Phase I; D050217:Metabolic Detoxication, Phase II; D018384:Oxidative Stress / Q000187:drug effects; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014260:Triclosan / Q000008:administration & dosage / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D000891:Anti-Infective Agents, Local; D029941:Fish Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014874:Water Pollutants, Chemical; D014260:Triclosan; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1; D051544:Cytochrome P-450 CYP3A; D005982:Glutathione Transferase
keywords: CYP; Detoxification; GST; Triclosan; Yellow catfish