title: MGr1-Ag/37LRP promotes growth and proliferation of gastric cancer in vitro and in vivo.
abstract: Gastric carcinoma (GC) is an aggressive cancer with a poor prognosis. We previously reported that MGr1-Ag was involved in multidrug resistance and anti-apoptosis in GC. However, the exact function of MGr1-Ag in GC proliferation is not clear. In this study, we found that MGr1-Ag was highly expressed in GC tissues and four GC cell lines compared with nontumor gastric tissues or gastric epithelial mucosa cells. The high expression of MGr1-Ag/37LRP was also consistent with the decreased median survival time of GC patients. We employed lenti-mediated RNA interference technique to knock down MGr1-Ag expression in SGC7901 and MKN45 cells, respectively, and observed its effects on GC cells growth in vitro and in vivo. Further study showed that knockdown of MGr1-Ag could inhibit GC cell proliferation by inhibiting the cell cycle S-phase entry and induced apoptosis. Soft agar colony formation assay indicated that the colony formation ability of SGC7901 and MKN45 cells decreased after lenti-MGr1-Ag small interfering RNA (siRNA) infection. Western blot revealed that cyclin D1 and Bcl-2 expression were downregulated whereas p27 and Bax were upregulated in lenti-MGr-siRNA-infected GC cells. Further study demonstrated that the proliferation effect of MGr1-Ag in GC is dependent on its laminin-binding region. Taken together, these data revealed a novel function of MGr1-Ag that can possibly be used as an independent prognostic factor and a potential therapeutic target for GC.
authors: Department of Oncology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
authors: L
authors: L
authors: Liu
authors: State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors: L
authors: L
authors: Sun
authors: State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors: K
authors: K
authors: Wu
authors: State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors: Y
authors: Y
authors: Shi
authors: Department of Oncology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
authors: Y
authors: Y
authors: Wang
authors: Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an, China.
authors: Y
authors: Y
authors: Wang
authors: Department of Oncology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
authors: N
authors: N
authors: Zhang
authors: State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors: H
authors: H
authors: Zhang
authors: Department of Oncology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
authors: H
authors: H
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D034622:RNA Interference; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D047368:Tumor Burden
chemical_list: D000951:Antigens, Neoplasm; C470229:MGr1-antigen, human