title: A single amino acid substitution in the novel H7N9 influenza A virus NS1 protein increases CPSF30 binding and virulence.
abstract: Although an effective interferon antagonist in human and avian cells, the novel H7N9 influenza virus NS1 protein is defective at inhibiting CPSF30. An I106M substitution in H7N9 NS1 can restore CPSF30 binding together with the ability to block host gene expression. Furthermore, a recombinant virus expressing H7N9 NS1-I106M replicates to higher titers in vivo, and is subtly more virulent, than the parental virus. Natural polymorphisms in H7N9 NS1 that enhance CPSF30 binding may be cause for concern.
authors: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: Juan
authors: J
authors: Ayllon
authors: MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.
authors: Patricia
authors: P
authors: Domingues
authors: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: Ricardo
authors: R
authors: Rajsbaum
authors: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: Lisa
authors: L
authors: Miorin
authors: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: Mirco
authors: M
authors: Schmolke
authors: MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom ben.hale@glasgow.ac.uk adolfo.garcia-sastre@mssm.edu.
authors: Benjamin G
authors: BG
authors: Hale
authors: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USA Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA ben.hale@glasgow.ac.uk adolfo.garcia-sastre@mssm.edu.
authors: Adolfo
authors: A
authors: García-Sastre
mesh_terms: D019943:Amino Acid Substitution; D000596:Amino Acids / Q000235:genetics*; D000818:Animals; D002645:Chickens; D015870:Gene Expression; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D014774:Virulence
chemical_list: D000596:Amino Acids; C085820:INS1 protein, influenza virus; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins