title: Phytochemical analysis and antileukemic activity of polyphenolic constituents of Toona sinensis.
abstract: Toona sinensis is a traditional Chinese medicine belonging to the Meliaceae family. The aim of this study was to identify the potential compounds responsible for anticancer activity of T. sinensis. The EtOAc extracts of leaves and woods of T. sinensis inhibited cell proliferation and induced apoptosis in human leukemia HL-60 cells. Our phytochemical research of these extracts led to the isolation of various polyphenolic constituents. The chemical structures were determined by spectroscopic analyses. Among isolates, gallic acid and loropetalin D showed inhibition of cell proliferation and possible induction of apoptosis in these cells. Overall, our results revealed the importance of T. sinensis as a chemopreventive medicinal plant. In addition, an analysis of structure-activity relationship indicated that the number of galloyl groups affects their antileukemic potency.
authors: Department of Materials Science and Technology, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors: Akinori
authors: A
authors: Kakumu
authors: Department of Materials Science and Technology, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors: Masayuki
authors: M
authors: Ninomiya
authors: Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Andalas, 25163 Limau Manis, Padang, Indonesia.
authors: Mai
authors: M
authors: Efdi
authors: Department of Chemistry, Faculty of Mathematics and Natural Science, Bengkulu University, Jalan Raya Kandang Limun, Bengkulu 38371, Indonesia.
authors: Morina
authors: M
authors: Adfa
authors: Division of Anaerobe Research, Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan.
authors: Masahiro
authors: M
authors: Hayashi
authors: Division of Anaerobe Research, Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan; United Graduate School of Drug Discovery and Medicinal Information Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan.
authors: Kaori
authors: K
authors: Tanaka
authors: Department of Materials Science and Technology, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. Electronic address: koketsu@gifu-u.ac.jp.
authors: Mamoru
authors: M
authors: Koketsu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D029582:Meliaceae / Q000737:chemistry*; D008968:Molecular Conformation; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013329:Structure-Activity Relationship; D014934:Wood / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D064209:Phytochemicals; D010936:Plant Extracts
keywords: Antileukemic activity; Degree of galloylation; Gallic acid; Loropetalin D; Toona sinensis