title: Analysis of the Vitellogenin gene of rice moth, Corcyra cephalonica Stainton.
abstract: Vitellogenin (Vg) is a precursor of the major yolk protein, an essential nutrient for the embryonic development of oviparous animals including insects. Here, the gene(CceVg [Corcyra cephalonica Vg] ) encoding the Vg (CceVg of moth, C. cephalonica, was cloned and sequenced. The gene sequence was 6,721-bp long and contained 5five introns and six exons that together formed a 5,382-bp open reading frame. The deduced protein (CceVg) consisted of 1,793 amino acid residues, including a 16-amino-acid signal peptide. The putative molecular weight of the primary Vg protein was 202.46 kDa. The CceVg contained all conserved domains and motifs that were commonly found in most insect Vgs except the presence of a polyserine tract at the C-terminal region, which had not been reported in other lepidopteran Vgs. The expression pattern showed that CceVg was first transcribed at a very low level in the early larval stage but disappeared in later stage larva. In female, the CceVg mRNA was detected in early pupal stage and throughout adult stage. Interestingly, the CceVg mRNA was detected only in mated males at low levels, not in the virgin ones. Injection of CceVg double-stranded RNA into early-emergent females caused severely abnormal ovaries.
authors: Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
authors: Mayura
authors: M
authors: Veerana
authors: Anchanee
authors: A
authors: Kubera
authors: Lertluk
authors: L
authors: Ngernsiri
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D007438:Introns; D007814:Larva / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010053:Ovary / Q000187:drug effects; D011679:Pupa / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification; D014819:Vitellogenins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D014819:Vitellogenins
keywords: Corcyra cephalonica; rice moth; vitellogenin