title: Lentiviral gene transduction of mouse and human hematopoietic stem cells.
abstract: Lentiviral vectors can be used to genetically modify a broad range of cells. Hematopoietic stem cells (HSCs) are particularly suitable for lentiviral gene augmentation, because these cells can be enriched with relative ease from mouse bone marrow and human hematopoietic sources, and in principle require relatively limited cell numbers to completely reconstitute the hematopoietic system in vivo. Furthermore, lentiviral vectors are very efficient if pseudotyped with broad tropism envelope proteins. This chapter focuses on gene modification by the use of self-inactivating third-generation human immunodeficiency virus-derived lentiviral vectors for ex vivo HSC modification for both mouse and human application.
authors: Department of Hematology, Erasmus University Medical Center, Dr. Molewaterplein 50, Room EE-1383a, 3015 GE, Rotterdam, The Netherlands.
authors: Niek P
authors: NP
authors: van Til
authors: Gerard
authors: G
authors: Wagemaker
mesh_terms: D000818:Animals; D005822:Genetic Vectors / Q000235:genetics; D057809:HEK293 Cells; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D016133:Polymerase Chain Reaction; D014161:Transduction, Genetic / Q000379:methods*