title: Metabolite identification of liguzinediol in dogs by ultra-flow liquid chromatography/tandem mass spectrometry.
abstract: Ultra-flow liquid chromatography/quadrupole-time-of-flight mass spectrometry (UFLC/Q-TOF MS) method combined with metabolitepilot(MT) software was used for analysis of the metabolites of liguzinediol in dogs. Urine, bile, feces and plasma samples were collected after intravenous administration of 8 mg/kg liguzinediol to healthy dogs. Besides liguzinediol, seven metabolites were detected and identified by UFLC/Q-TOF MS method. The results showed that liguzinediol had some main metabolic pathways in dogs including oxidation, sulfation, cysteine conjugation, N-acetylcysteine conjugation and glucuronidation.
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210029, People's Republic of China.
authors: Chen-xiao
authors: CX
authors: Shan
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210029, People's Republic of China.
authors: Wei
authors: W
authors: Li
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210029, People's Republic of China. Electronic address: njwenhm@126.com.
authors: Hong-mei
authors: HM
authors: Wen
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210029, People's Republic of China.
authors: Xin-zhi
authors: XZ
authors: Wang
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210029, People's Republic of China.
authors: Xiao-wen
authors: XW
authors: Zhu
authors: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210029, People's Republic of China.
authors: Xiao-bin
authors: XB
authors: Cui
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D005243:Feces / Q000737:chemistry; D053858:Metabolic Networks and Pathways; D011719:Pyrazines / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C572935:2,5-dihydroxymethyl-3,6-dimethylpyrazine; D011719:Pyrazines
keywords: Beagle dog; Liguzinediol; Metabolites; UFLC/Q-TOF MS