title: The effect of different drugs on the preparation and biological outcomes of plasma rich in growth factors.
abstract: Chronic diseases are the major contributors to the global burden of disease and involve prodigious consumption of various drugs that usually affect platelet function. The autologous technology of plasma rich in growth factors (PRGF) provides a biological approach using autologous platelets as a reservoir and local delivery of proteins to promote tissue healing. The purpose of this study was to evaluate the effect of the consumption of acetylsalicylic acid, acenocoumarol and glucosamine sulfate on the preparation as well as on the biological properties of the PRGF technology. Clotting time and platelet activation of PRGF was evaluated. The latter was performed by flow cytometry. PRGF growth factor content and the release of various biomolecules by gingival fibroblasts were quantified by enzyme-linked immunosorbent assay. Cell proliferation was evaluated by means of a fluorescence-based method and cell migration was performed on culture inserts. None of the parameters evaluated was modified by the consumption of any of the three drugs tested; only the plasma of patients who had consumed acetylsalicylic acid and acenocoumarol expressed greater gingival fibroblast migration compared to plasma control. The intake of acetylsalicylic acid, acenocoumarol and glucosamine sulfate does not alter the preparation and biological properties of the autologous technology of PRGF.
authors: Private Practice in Implantology and Oral Rehabilitation in Vitoria, Spain; Eduardo Anitua Foundation, c/José María Cagigal 19, Vitoria 01007, Spain.
authors: Eduardo
authors: E
authors: Anitua
authors: Eduardo Anitua Foundation, c/José María Cagigal 19, Vitoria 01007, Spain.
authors: María
authors: M
authors: Troya
authors: Eduardo Anitua Foundation, c/José María Cagigal 19, Vitoria 01007, Spain.
authors: Mari Mar
authors: MM
authors: Zalduendo
authors: Eduardo Anitua Foundation, c/José María Cagigal 19, Vitoria 01007, Spain. Electronic address: gorka.orive@ehu.es.
authors: Gorka
authors: G
authors: Orive
mesh_terms: D000074:Acenocoumarol / Q000097:blood / Q000494:pharmacology*; D000368:Aged; D000369:Aged, 80 and over; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood / Q000494:pharmacology; D000925:Anticoagulants / Q000097:blood / Q000494:pharmacology; D001241:Aspirin / Q000097:blood / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology*; D010975:Platelet Aggregation Inhibitors / Q000097:blood / Q000494:pharmacology; D053657:Platelet-Rich Plasma / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000925:Anticoagulants; D036341:Intercellular Signaling Peptides and Proteins; D010975:Platelet Aggregation Inhibitors; D000074:Acenocoumarol; D001241:Aspirin
keywords: Acenocoumarol; Acetylsalicylic acid; Gingival fibroblasts; Glucosamine sulfate; Growth factors; Plasma rich in growth factors