title: Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS.
abstract: MALDI-TOF MS has been evaluated to detect carbapenemases activity and pathogen identification directly from positive blood cultures. 21 non-carbapenemase producers and 19 carbapenemase producers Enterobacteriaceae and Pseudomonas aeruginosa strains were included in the study. This technique is simple and detects carbapenemases in 4.5h with high sensitivity and specificity.
authors: Department of Microbiology, University Hospital Virgen de las Nieves, Granada, Spain; Clinical Medicine and Public Health, Postgraduate programme, University of Granada, Granada, Spain.
authors: Y
authors: Y
authors: Hoyos-Mallecot
authors: Department of Microbiology, University Hospital Virgen de las Nieves, Granada, Spain. Electronic address: riazzoc@gmail.com.
authors: C
authors: C
authors: Riazzo
authors: Department of Microbiology, University Hospital Virgen de las Nieves, Granada, Spain.
authors: C
authors: C
authors: Miranda-Casas
authors: Department of Microbiology, University Hospital Virgen de las Nieves, Granada, Spain.
authors: M D
authors: MD
authors: Rojo-Martín
authors: Department of Microbiology, University Hospital Virgen de las Nieves, Granada, Spain; Clinical Medicine and Public Health, Postgraduate programme, University of Granada, Granada, Spain.
authors: J
authors: J
authors: Gutiérrez-Fernández
authors: Department of Microbiology, University Hospital Virgen de las Nieves, Granada, Spain.
authors: J M
authors: JM
authors: Navarro-Marí
mesh_terms: D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Blood culture; Carbapenemases; MALDI-TOF