title: MicroRNA-204, a direct negative regulator of ezrin gene expression, inhibits glioma cell migration and invasion.
abstract: Ezrin is overexpressed in a variety of neoplastic cells and involved in the later stages of tumor progression and metastasis. Ezrin expression can be regulated at both the transcriptional and post-transcriptional levels. We used a combination of bioinformatics and experimental techniques to demonstrate that the miR-204 is a direct negative regulator of ezrin. Overexpression of miR-204 mimics decreased the activity of a luciferase reporter containing the ezrin 3' UTR and led to repression of ezrin protein. In contrast, ectopic expression of miR-204 inhibitor elevated ezrin expression. We also show that miR-204 is down-regulated in a panel of glioma tissues and in high invasive glioma cell lines we examined. Moreover, miR-204 mimics significantly reduced glioma cell migration and invasion, while miR-204 inhibitor generated the opposite results. Finally, overexpression of miR-204 and knockdown of ezrin reduced glioma cell invasion, and these effects could be rescued by re-expression of ezrin. These findings reveal that miR-204 could be partly due to its inhibitory effects on glioma cell migration and invasion through regulating ezrin expression.
authors: Department of Neurosurgery of The first affiliated Hospital, Wannan Medical College, 2 West Zheshan Road, Wuhu, 241001, Anhui, People's Republic of China.
authors: Jie
authors: J
authors: Mao
authors: Mengying
authors: M
authors: Zhang
authors: Min
authors: M
authors: Zhong
authors: Yingying
authors: Y
authors: Zhang
authors: Kun
authors: K
authors: Lv
mesh_terms: D020413:3' Untranslated Regions; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D002465:Cell Movement; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D000099149:Ezrin
chemical_list: D020413:3' Untranslated Regions; D003598:Cytoskeletal Proteins; D035683:MicroRNAs; D000099149:Ezrin; C549404:MIRN204 microRNA, human