title: Second generation codon optimized minicircle (CoMiC) for nonviral reprogramming of human adult fibroblasts.
abstract: The ability to induce pluripotency in somatic cells is one of the most important scientific achievements in the fields of stem cell research and regenerative medicine. This technique allows researchers to obtain pluripotent stem cells without the controversial use of embryos, providing a novel and powerful tool for disease modeling and drug screening approaches. However, using viruses for the delivery of reprogramming genes and transcription factors may result in integration into the host genome and cause random mutations within the target cell, thus limiting the use of these cells for downstream applications. To overcome this limitation, various non-integrating techniques, including Sendai virus, mRNA, minicircle, and plasmid-based methods, have recently been developed. Utilizing a newly developed codon optimized 4-in-1 minicircle (CoMiC), we were able to reprogram human adult fibroblasts using chemically defined media and without the need for feeder cells.
authors: Lorry I. Lokey Stem Cell Research Building, Stanford University School of Medicine, 265 Campus Drive, Room G1105, Stanford, CA, 94305-5454, USA.
authors: Sebastian
authors: S
authors: Diecke
authors: Leszek
authors: L
authors: Lisowski
authors: Nigel G
authors: NG
authors: Kooreman
authors: Joseph C
authors: JC
authors: Wu
mesh_terms: D000328:Adult; D001706:Biopsy; D002469:Cell Separation; D065150:Cellular Reprogramming; D003062:Codon / Q000235:genetics*; D003094:Collagen / Q000494:pharmacology; D004338:Drug Combinations; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005821:Genetic Techniques; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D007797:Laminin / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D011509:Proteoglycans / Q000494:pharmacology; D012867:Skin / Q000473:pathology; D014162:Transfection
chemical_list: D003062:Codon; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen