title: Label-free electrochemiluminescent enantioselective sensor for distinguishing between chiral metallosupramolecular complexes.
abstract: Chiral molecular recognition of DNA is important for rational drug design and for developing structural probes of DNA conformation. Developing a convenient and inexpensive assay for sensitive and selective identification of DNA-specific binding compounds with rapid, easy manipulation is in ever-increasing demand. Here, we present a "turn-on" and label-free electrochemiluminescent (ECL) biosensor for distinguishing chiral metallosupramolecular complexes based on DNA three-way junction formation selectively induced by the analyte. The fabricated ECL sensor shows excellent performance in the chiral discrimination of two enantiomers with an enantioselective recognition ratio of up to 4.4. More importantly, as a "turn-on" detection system, the ECL chiral sensor does not suffer from false positives and limited signal range of "signal-off" systems. Therefore, this concept may provide a new insight into the design of efficient sensors for distinguishing chiral molecules and for investigating the interactions between DNA and small molecules.
authors: Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, Jilin 130022 (R.P. China); University of the Chinese Academy of Science, Beijing, 100039 (R.P. China).
authors: Li
authors: L
authors: Wu
authors: Chuanqi
authors: C
authors: Zhao
authors: Jinsong
authors: J
authors: Ren
authors: Xiaogang
authors: X
authors: Qu
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D008958:Models, Molecular; D013237:Stereoisomerism
chemical_list: D056831:Coordination Complexes
keywords: DNA; chirality; electrochemiluminescence; enantioselective recognition; metallosupramolecules; three-way junctions