title: Expression pattern of clinically relevant markers in paediatric germ cell- and sex-cord stromal tumours is similar to adult testicular tumours.
abstract: Paediatric germ cell tumours (GCTs) are rare and account for less than 3 % of childhood cancers. Like adult GCTs, they probably originate from primordial germ cells, but the pattern of histopathological types is different, and they occur predominantly in extragonadal sites along the body midline. Because they are rare, histology of paediatric GCTs is poorly documented, and it remains unclear to what extent they differ from adult GCTs. We have analysed 35 paediatric germ cell tumours and 5 gonadal sex-cord stromal tumours from prepubertal patients aged 0-15 years, to gain further knowledge, elaborate on clinical-pathological associations and better understand their developmental divergence. The tumours were screened for expression of stemness-related factors (OCT4, AP-2γ, SOX2), classical yolk sac tumours (YSTs; AFP, SALL4), GCTs (HCG, PLAP, PDPN/D2-40), as well as markers for sex-cord stromal tumour (PDPN, GATA4). All YSTs expressed AFP and SALL4, with GATA4 present in 13/14. The majority of teratomas expressed SOX2 and PDPN, whereas SALL4 was found in 8/13 immature teratomas. Adult seminoma markers AP-2γ, OCT4, SALL4 and PDPN were all expressed in dysgerminoma. We further report a previously unrecognised pathogenetic relationship between AFP and SALL4 in YST in that different populations of YST cells express either SALL4 or AFP, which suggests variable differentiation status. We also show that AP-2γ is expressed in the granulosa layer of ovarian follicles and weakly expressed in immature but not in mature granulosa cell tumours. Our findings indicate that the expression pattern of these antigens is similar between paediatric and adult GCTs, even though they develop along different developmental trajectories.
authors: Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.
authors: Christiane Hammershaimb
authors: CH
authors: Mosbech
authors: Terje
authors: T
authors: Svingen
authors: John Erik
authors: JE
authors: Nielsen
authors: Birgitte Groenkaer
authors: BG
authors: Toft
authors: Catherine
authors: C
authors: Rechnitzer
authors: Bodil Laub
authors: BL
authors: Petersen
authors: Ewa
authors: E
authors: Rajpert-De Meyts
authors: Christina Engel
authors: CE
authors: Hoei-Hansen
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002648:Child; D002675:Child, Preschool; D018240:Endodermal Sinus Tumor / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009373:Neoplasms, Germ Cell and Embryonal / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism; D018239:Seminoma / Q000378:metabolism; D013724:Teratoma / Q000378:metabolism*; D013736:Testicular Neoplasms / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis; D000509:alpha-Fetoproteins / Q000096:biosynthesis
chemical_list: C097353:AFP protein, human; D014408:Biomarkers, Tumor; C472575:SALL4 protein, human; D014157:Transcription Factors; D000509:alpha-Fetoproteins