title: Effective analysis of the use of peracetic acid after instrumentation of root canals contaminated with Enterococcus faecalis.
abstract: BACKGROUND
  The aim of this study was to evaluate the effectiveness of peracetic acid (PAA) in cleaning root canals contaminated with Enterococcus faecalis.
  
  
  METHODS
  Sixty first and second mandibular molars were used. Their mesiobuccal canals were prepared with the Reciproc System (VDW, Munich, Germany). The canals were irrigated with 10 mL saline during instrumentation. The teeth were randomly divided into 3 groups (n = 20), according to the irrigation solution to be used after instrumentation: group PAA (5 mL 1% PAA), group EDTA/sodium hypochlorite (NaOCl) (5 mL 17% EDTA followed by 5 mL 2.5% sodium hypochlorite), and group S (5 mL saline). Microbiological samples were collected before instrumentation and after final irrigation. Bacterial quantification was performed by counting the number of colony-forming units (CFUs/mL). The results were analyzed by the nonparametric Wilcoxon and Kruskal-Wallis tests.
  
  
  RESULTS
  The 3 groups showed a significant reduction (P < .05) in CFUs/mL after final irrigation. PAA and NaOCl associated with EDTA produced a significantly higher reduction in CFUs/mL (P < .05) compared with saline. There was no statistically significant difference between PAA and EDTA + 2.5% NaOCl (P > .05).
  
  
  CONCLUSIONS
  According to the results of the present study, the effectiveness of 1% PAA was similar to that of 17% EDTA + 2.5% NaOCl in cleaning curved root canals contaminated with E. faecalis.
authors: São Leopoldo Mandic Dental Research Center, Campinas, São Paulo, Brazil.
authors: Caroline Berwanger
authors: CB
authors: Cord
authors: São Leopoldo Mandic Dental Research Center, Campinas, São Paulo, Brazil.
authors: Rafael Vidal Cortez
authors: RV
authors: Velasco
authors: São Leopoldo Mandic Dental Research Center, Campinas, São Paulo, Brazil.
authors: Laíla Fernanda
authors: LF
authors: Ribeiro Melo Lima
authors: São Leopoldo Mandic Dental Research Center, Campinas, São Paulo, Brazil.
authors: Daniel Guimarães Pedro
authors: DG
authors: Rocha
authors: São Leopoldo Mandic Dental Research Center, Campinas, São Paulo, Brazil.
authors: Carlos Eduardo
authors: CE
authors: da Silveira Bueno
authors: Pontifical Catholic University of Campinas, Campinas, São Paulo, Brazil. Electronic address: slpinho@puc-campinas.edu.br.
authors: Sérgio Luiz
authors: SL
authors: Pinheiro
mesh_terms: D058491:Bacterial Load / Q000187:drug effects; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004492:Edetic Acid / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D050296:Microbial Viability / Q000187:drug effects; D008963:Molar / Q000382:microbiology; D010463:Peracetic Acid / Q000494:pharmacology*; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000295:instrumentation; D012973:Sodium Hypochlorite / Q000494:pharmacology; D007507:Therapeutic Irrigation / Q000295:instrumentation; D019227:Tooth Apex / Q000382:microbiology
chemical_list: D012388:Root Canal Irrigants; D004492:Edetic Acid; D012973:Sodium Hypochlorite; D010463:Peracetic Acid
keywords: Enterococcus faecalis; peracetic acid; root canal irrigants