title: Global transcriptomic analysis uncovers a switch to anaerobic metabolism in tellurite-exposed Escherichia coli.
abstract: Tellurite (TeO3(2-)) is harmful for most microorganisms, especially Gram-negative bacteria. Even though tellurite toxicity involves a number of individual aspects, including oxidative stress, malfunctioning of metabolic enzymes and a drop in the reduced thiol pool, among others, the general mechanism of toxicity is rather complex and not completely understood to date. This work focused on DNA microarray analysis to evaluate the Escherichia coli global transcriptomic response when exposed to the toxicant. Confirming previous results, the induction of the oxidative stress response regulator soxS was observed. Upregulation of a number of genes involved in the global stress response, protein folding, redox processes and cell wall organization was also detected. In addition, downregulation of aerobic respiration-related genes suggested a metabolic switch to anaerobic respiration. The expression results were validated through oxygen consumption experiments, which corroborated that tellurite-exposed cells effectively consume oxygen at lower rates than untreated controls.
authors: Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Chile; Laboratorio de Microbiología, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Chile.
authors: Roberto C
authors: RC
authors: Molina-Quiroz
authors: Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Chile; Centro Nacional de Genómica y Bioinformática, Santiago, Chile.
authors: David E
authors: DE
authors: Loyola
authors: Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Chile; Facultad de Ciencia, Universidad San Sebastián, Santiago, Chile.
authors: Waldo A
authors: WA
authors: Díaz-Vásquez
authors: Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Chile.
authors: Felipe A
authors: FA
authors: Arenas
authors: Laboratorio de Genómica Aplicada, ICBM, Facultad de Medicina, Universidad de Chile, Chile.
authors: Ulises
authors: U
authors: Urzúa
authors: Bionanotechnology and Microbiology Lab, Center for Bioinformatics and Integrative Biology (CBIB), Universidad Andres Bello, República 239, Santiago, Chile.
authors: José M
authors: JM
authors: Pérez-Donoso
authors: Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Chile. Electronic address: claudio.vasquez@usach.cl.
authors: Claudio C
authors: CC
authors: Vásquez
mesh_terms: D000693:Anaerobiosis; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D046228:Microarray Analysis; D010100:Oxygen / Q000378:metabolism; D013691:Tellurium / Q000633:toxicity*
chemical_list: C026660:tellurous acid; D013691:Tellurium; D010100:Oxygen
keywords: Anaerobic respiration; Microarrays; Oxidative stress; Tellurite