title: Acinetobacter bohemicus sp. nov. widespread in natural soil and water ecosystems in the Czech Republic.
abstract: We investigated the taxonomic status of a phenetically unique group of 25 Acinetobacter strains which were isolated from multiple soil and water samples collected in natural ecosystems in the Czech Republic. Based on the comparative sequence analyses of the rpoB, gyrB, and 16S rRNA genes, the strains formed a coherent and well separated branch within the genus Acinetobacter. The genomic uniqueness of the group at the species level was supported by the low average nucleotide identity values (≤77.37%) between the whole genome sequences of strain ANC 3994(T) (NCBI accession no. APOH00000000) and the representatives of the known Acinetobacter species. Moreover, all 25 strains created a tight cluster clearly separated from all hitherto described species based on whole-cell protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and shared a unique combination of metabolic and physiological properties. The capacity to assimilate l-histidine and the inability to grow at 35°C differentiated them from their phenotypically closest neighbor, Acinetobacter johnsonii. We conclude that the 25 strains represent a novel Acinetobacter species, for which the name Acinetobacter bohemicus sp. nov. is proposed. The type strain of A. bohemicus is ANC 3994(T) (=CIP 110496(T)=CCUG 63842(T)=CCM 8462(T)).
authors: Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, 100 42 Prague 10, Czech Republic.
authors: Lenka
authors: L
authors: Krizova
authors: Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, 100 42 Prague 10, Czech Republic.
authors: Martina
authors: M
authors: Maixnerova
authors: Research Group Proteomics, Central European Institute of Technology and National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors: Ondrej
authors: O
authors: Sedo
authors: Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, 100 42 Prague 10, Czech Republic. Electronic address: anemec@szu.cz.
authors: Alexandr
authors: A
authors: Nemec
mesh_terms: D000150:Acinetobacter / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D018153:Czech Republic; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D017753:Ecosystem; D006639:Histidine / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D006639:Histidine; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D027081:DNA Gyrase
keywords: 16S rRNA gene; ANI; Carbon source assimilation; MALDI-TOF MS; gyrB; rpoB