title: Saccharopolyspora halotolerans sp. nov., a halophilic actinomycete isolated from a hypersaline lake.
abstract: A novel actinomycete strain, designated TRM 45123(T), was isolated from a hypersaline habitat in Xinjiang Province (40° 20' N 90° 49' E), north-west China. The isolate was characterized using a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain TRM 45123(T) belonged to the genus Saccharopolyspora and was closely related to Saccharopolyspora gloriosae (96.7% similarity). The G+C content of the DNA was 69.07 mol%. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid, and arabinose and ribose as the major whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine and phosphatidylglycerol. The predominant menaquinone was MK-9(H4). The major fatty acids were iso-C16 : 0, anteiso-C17:0, iso-C15:0 and anteiso-C15:0. On the basis of the evidence from this polyphasic study, a novel species, Saccharopolyspora halotolerans sp. nov., is proposed. The type strain of Saccharopolyspora halotolerans is TRM 45123(T) ( = CCTCC AA 2013006(T) = DSM 45990(T)).
authors: Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Ling-Ling
authors: LL
authors: Lv
authors: College of Life Science, Tarim University, Alar 843300, PR China.|Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.
authors: Yue-Feng
authors: YF
authors: Zhang
authors: College of Life Science, Tarim University, Alar 843300, PR China.|Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.
authors: Zhan-Feng
authors: ZF
authors: Xia
authors: College of Life Science, Tarim University, Alar 843300, PR China.
authors: Jing-Jing
authors: JJ
authors: Zhang
authors: College of Life Science, Tarim University, Alar 843300, PR China.|Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors: Li-Li
authors: LL
authors: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D017753:Ecosystem; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016930:Saccharopolyspora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D054712:Salinity; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid