title: A rapid method to determine colonic microbial metabolites derived from grape flavanols in rat plasma by liquid chromatography-tandem mass spectrometry.
abstract: This study describes the development and validation of a liquid chromatography-mass spectrometry method for determination of a large number of flavanol colonic derivatives in biological samples. The method was validated with rat plasma after the intake of grape seed flavanols. The minimum plasma volume necessary to maintain good recovery values within the range of 83-110% for all of the standards was determined by micro solid-phase extraction (μ-SPE). In total, 16 commercial standards were used to measure 30 different phenolic compounds present at low concentration levels (micromolar). The chromatographic method enabled reliable quantification of plasma colonic flavanol derivatives with low limits of detection and quantification, achieving values of 0.03 nM and 0.10 nM, respectively. The developed method can be readily applied to determine all of the flavanol metabolites that are most likely responsible for the majority of biological effects of poorly absorbed flavanols.
authors: Nutrigenomic Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili , Tarragona 43007, Spain.
authors: Maria
authors: M
authors: Margalef
authors: Zara
authors: Z
authors: Pons
authors: Begoña
authors: B
authors: Muguerza
authors: Anna
authors: A
authors: Arola-Arnal
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003106:Colon / Q000382:microbiology*; D005419:Flavonoids / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D056604:Grape Seed Extract / Q000008:administration & dosage / Q000737:chemistry; D008297:Male; D059808:Polyphenols / Q000032:analysis / Q000378:metabolism; D044945:Proanthocyanidins / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D052617:Solid Phase Microextraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D027843:Vitis / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D056604:Grape Seed Extract; D059808:Polyphenols; D044945:Proanthocyanidins