title: Guanidyl-functionalized graphene as a bifunctional adsorbent for selective enrichment of phosphopeptides.
abstract: Guanidyl-functionalized graphene (GFG) was designed and synthesized. By simple modulation of the loading buffer this novel bifunctional adsorbent provides two enriching functions, one for global phosphopeptides, and the other for multiphosphopeptides with consecutive phosphorylated residues. GFG provides a promising perspective for consecutive phosphorylated peptide enrichment in phosphor-proteomic research and related biological process studies.
authors: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Institute of Analytical Chemistry, Peking University, Beijing 100871, China. yu.bai@pku.edu.cn.
authors: Lin-Nan
authors: LN
authors: Xu
authors: Li-Ping
authors: LP
authors: Li
authors: Liang
authors: L
authors: Jin
authors: Yu
authors: Y
authors: Bai
authors: Hu-Wei
authors: HW
authors: Liu
mesh_terms: D002364:Caseins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006108:Graphite / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010748:Phosphopeptides / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
chemical_list: D002364:Caseins; D010455:Peptides; D010748:Phosphopeptides; C015462:polyarginine; D006147:Guanine; D006108:Graphite; D014357:Trypsin