title: Infection of Manila clams Ruditapes philippinarum from Galicia (NW Spain) with a Mikrocytos-like parasite.
abstract: The name 'microcells' is frequently used to refer to small-sized unicellular stages of molluscan parasites of the genera Bonamia (Rhizaria, Haplosporidia) and Mikrocytos (Rhizaria). Histological examination of Manila clams Ruditapes philippinarum revealed microcells in the connective tissue of adductor muscle, foot, mantle, gills, siphon and visceral mass. The clams had been collected from 4 beds on the coast of Galicia, Spain. The prevalence of these microcells ranged from 73 to 93% in surface clams and from 3 to 33% in buried clams. However, the detection of brown ring disease signs in clams from every bed prevented us from making the assumption that the microcells alone were responsible for clam mortality. PCR assays using primer pairs designed to detect Bonamia spp. and haplosporidians gave negative results, whereas positive results were obtained with primers for the genus Mikrocytos. A consensus sequence of 1670 bp of the ribosomal gene complex of the microcells was obtained. It contained a section of the 18S region, the whole first internal transcribed spacer, the 5.8S region, the second internal transcribed spacer and a section of the 28S region. Comparison of this sequence with those of M. mackini infecting Crassostrea gigas and Mikrocytos sp. infecting Ostrea edulis showed that the microcells of Galician clams were the most divergent among the compared parasites. This is the first report of a Mikrocytos-like parasite infecting Manila clams. Care must be taken to avoid the spread of this parasite through Manila clam transfers.
authors: Centro de Investigacións Mariñas, Consellería do Medio Rural e do Mar, Xunta de Galicia, 36620 Vilanova de Arousa, Spain.
authors: Andrea
authors: A
authors: Ramilo
authors: David
authors: D
authors: Iglesias
authors: Elvira
authors: E
authors: Abollo
authors: Mar
authors: M
authors: González
authors: Susana
authors: S
authors: Darriba
authors: Antonio
authors: A
authors: Villalba
mesh_terms: D000818:Animals; D001267:Atlantic Ocean; D049872:Bivalvia / Q000469:parasitology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D056890:Eukaryota / Q000235:genetics / Q000502:physiology*; D006790:Host-Parasite Interactions; D016133:Polymerase Chain Reaction; D013030:Spain; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer; D004247:DNA