title: Electrochemical enzymatic biosensor with long-term stability using hybrid mesoporous membrane.
abstract: An acetylcholinesterase-immobilized sensor unit was successfully prepared by encapsulating the enzyme within hybrid mesoporous silica membranes (F127-MST). Through a novel combination with tetracyanoquinodimethane, both acetylcholine and organophosphorus pesticides were successfully detected with high sensitivity. Furthermore, we manufactured the working prototype of an enzyme sensor with this sensor unit for detecting dichlorvos, aldicarb and parathion. At present, the detection limit in this working prototype either equaled or surpassed that of others. Also, we have the advantage of increased stability of the enzyme against the outer environment by encapsulation of the enzymes into a silica nanospace. Consequently, acetylcholinesterase immobilized in F127-MST is a practical sensor with high sensitivity, reusability, and storage stability.
authors: National Institute of Advanced Industrial Science and Technology (AIST), Nigatake 4-2-1, Miyagino-ku, Sendai, 983-8551, Japan. t-itoh@ni.aist.go.jp.
authors: Tetsuji
authors: T
authors: Itoh
authors: Takeshi
authors: T
authors: Shimomura
authors: Akari
authors: A
authors: Hayashi
authors: Akira
authors: A
authors: Yamaguchi
authors: Norio
authors: N
authors: Teramae
authors: Masatoshi
authors: M
authors: Ono
authors: Tatsuo
authors: T
authors: Tsunoda
authors: Fujio
authors: F
authors: Mizukami
authors: Galen D
authors: GD
authors: Stucky
authors: Taka-aki
authors: TA
authors: Hanaoka
mesh_terms: D000109:Acetylcholine / Q000032:analysis* / Q000378:metabolism; D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004593:Electrophorus; D004800:Enzymes, Immobilized / Q000378:metabolism; D004867:Equipment Design; D057230:Limit of Detection; D008567:Membranes, Artificial; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000378:metabolism; D010575:Pesticides / Q000032:analysis* / Q000378:metabolism; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009943:Organophosphorus Compounds; D010575:Pesticides; D012822:Silicon Dioxide; D000110:Acetylcholinesterase; D000109:Acetylcholine