title: A new Rhodamine B-based 'on-off' chemical sensor with high selectivity and sensitivity toward Fe(3+) and its imaging in living cells.
abstract: A new fluorescent chemosensor based on a Rhodamine B and pyrrole conjugate (RBPY) has been designed and synthesized. UV-vis absorption and fluorescence spectroscopic studies show that RBPY exhibits a high selectivity and sensitivity toward Fe(3+) among many other metal cations in a MeOH/H2O solution (3:2, v/v, pH 7.10, HEPES buffer, 0.1mM) by forming a 1:1 complex with Fe(3+). Furthermore, results reveal that the formation of the RBPY-Fe(3+) complex is fully reversible in the presence of sulfide anions and could also be used as an efficient sensor for S(2-). Importantly, fluorescence microscopy experiments further demonstrated that RBPY can be utilized as a fluorescent probe for the detection of Fe(3+) in human liver (L-02) cells.
authors: Department of Biochemical Engineering, Nanjing University of Science & Technology, Chemical Engineering Building B308, 200 Xiaolinwei, Nanjing 210094, PR China. Electronic address: baoxiaofeng@mail.njust.edu.cn.
authors: Xiaofeng
authors: X
authors: Bao
authors: School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing 210094, PR China.
authors: Jiaxin
authors: J
authors: Shi
authors: School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing 210094, PR China.
authors: Xuemei
authors: X
authors: Nie
authors: School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing 210094, PR China.
authors: Baojing
authors: B
authors: Zhou
authors: School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing 210094, PR China.
authors: Xinlong
authors: X
authors: Wang
authors: Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: lyzhang@cpu.edu.cn.
authors: Luyong
authors: L
authors: Zhang
authors: Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, PR China.
authors: Hong
authors: H
authors: Liao
authors: Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, PR China.
authors: Tao
authors: T
authors: Pang
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D005290:Ferric Compounds / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D057054:Molecular Imaging; D015394:Molecular Structure; D011789:Quantum Theory; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D005456:Fluorescent Dyes; D012235:Rhodamines; C029773:rhodamine B
keywords: Cell imaging; Chemosensor; Fe(III) ions; Fluorescence; Rhodamine B derivative