title: IgE binding to peanut allergens is inhibited by combined D-aspartic and D-glutamic acids.
abstract: The objective of this study was to determine if D-amino acids (D-aas) bind and inhibit immunoglobulin E (IgE) binding to peanut allergens. D-aas such as D-Asp (aspartic acid), D-Glu (glutamic acid), combined D-[Asp/Glu] and others were each prepared in a cocktail of 9 other D-aas, along with L-amino acids (L-aas) and controls. Each sample was mixed with a pooled plasma from peanut-allergic donors, and tested by ELISA (enzyme-linked immunosorbent assay) and Western blots for IgE binding to peanut allergens. Results showed that D-[Asp/Glu] (4 mg/ml) inhibited IgE binding (75%) while D-Glu, D-Asp and other D-aas had no inhibitory effect. A higher inhibition was seen with D-[Asp/Glu] than with L-[Asp/Glu]. We concluded that IgE was specific for D-[Asp/Glu], not D-Asp or D-Glu, and that D-[Asp/Glu] was more reactive than was L-[Asp/Glu] in IgE inhibition. The finding indicates that D-[Asp/Glu] may have the potential for removing IgE or reducing IgE binding to peanut allergens in vitro.
authors: United States Department of Agriculture, Agricultural Research Services, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA. Electronic address: siyin.chung@ars.usda.gov.
authors: Si-Yin
authors: SY
authors: Chung
authors: United States Department of Agriculture, Agricultural Research Services, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA.
authors: Shawndrika
authors: S
authors: Reed
mesh_terms: D000485:Allergens / Q000276:immunology*; D010367:Arachis / Q000737:chemistry / Q000276:immunology*; D001224:Aspartic Acid / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005971:Glutamates / Q000737:chemistry*; D007073:Immunoglobulin E / Q000276:immunology*
chemical_list: D000485:Allergens; D005971:Glutamates; D001224:Aspartic Acid; D007073:Immunoglobulin E
keywords: Ara h 1 and Ara h 2; IgE antibodies; Peanut allergens; d-Amino acids; d-Aspartic acid; d-Glutamic acid