title: Comparison of two commercial real-time PCR assays for detection of dengue virus in patient serum samples.
abstract: This study evaluated and compared the performance of two real-time PCR assays using nested reverse transcription (RT)-PCR as the reference method. Among 117 nested RT-PCR-positive cases, the abTES DEN 5 qPCR kit detected 97.4% of dengue virus (DENV) infections, while the innuDETECT Dengue TwoStep assay did so for 44.4%. Sensitivity varied by infecting serotype and the stage of infection. The abTES kit has the potential to replace nested RT-PCR for the rapid diagnosis of DENV infection.
authors: Graduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumtani, Thailand.
authors: J
authors: J
authors: Saengsawang
authors: Graduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumtani, Thailand oytipntl@hotmail.com.
authors: O
authors: O
authors: Nathalang
authors: Department of Pathology, Phramongkutklao Hospital, Bangkok, Thailand.
authors: M
authors: M
authors: Kamonsil
authors: Department of Pediatrics, Phramongkutklao Hospital, Bangkok, Thailand.
authors: V
authors: V
authors: Watanaveeradej
mesh_terms: D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology*; D013997:Time Factors