title: In vitro antioxidant capacity of tea of Echinodorus grandiforus, "leather hat," in Wistar rat liver.
abstract: Oxidative stress has been considered as one of the factors responsible for hepatic diseases, which sometimes require new ways of treatment. The present study aimed to evaluate the in vitro antioxidant capacity of the tea of Echinodorus grandiforus ("leather hat" plant) in rat liver. Different preparations of tea were evaluated for phenolic composition, antioxidant activity by DPPH assay and ability to inhibit lipid peroxidation induced by copper sulfate. The antioxidant activity was assessed in liver tissue treated with sodium azide in the presence or absence of tea by assays for lipid peroxidation (TBARS), protein oxidation (carbonyl) and the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD). The results show that different preparations of tea are important sources of polyphenols and contain theobromine, catechin and vitexin. Furthermore, the results indicate that this tea exhibits an antioxidant activity by its ability to scavenge DPPH radical. Different preparations of tea prevented damage to lipids and proteins induced by sodium azide, as well as assisting in restoring CAT and SOD activities. Thus, it can be seen that E. grandiforus tea had antioxidant activity in serum and liver being able to prevent oxidative damages generated by sodium azide.
authors: Centro Universitário Metodista, IPA, Centro de Pesquisas, Laboratório de Bioquímica, Porto Alegre, RS, Brasil.
authors: Rafaela F
authors: RF
authors: Lunardi
authors: Centro Universitário Metodista, IPA, Centro de Pesquisas, Laboratório de Bioquímica, Porto Alegre, RS, Brasil.
authors: Mariane
authors: M
authors: Wohlenberg
authors: Centro Universitário Metodista, IPA, Centro de Pesquisas, Laboratório de Bioquímica, Porto Alegre, RS, Brasil.
authors: Niara
authors: N
authors: Medeiros
authors: Universidade de Caxias do Sul, UCS, Laboratório de Óleos Essenciais, Caxias do Sul, RS, Brasil.
authors: Fabiane
authors: F
authors: Agostini
authors: Centro Universitário Metodista, IPA, Centro de Pesquisas, Laboratório de Bioquímica, Porto Alegre, RS, Brasil.
authors: Cláudia
authors: C
authors: Funchal
authors: Centro Universitário Metodista, IPA, Centro de Pesquisas, Laboratório de Bioquímica, Porto Alegre, RS, Brasil.
authors: Caroline
authors: C
authors: Dani
mesh_terms: D034901:Alismataceae / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001628:Beverages; D002374:Catalase / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000201:enzymology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D000975:Antioxidants; D002374:Catalase; D013482:Superoxide Dismutase