title: The influence of radiotherapy on IL-2 and IL-6 secretions of mucous membrane epithelial cells of wistar small intestine.
abstract: The aim of the study was to investigate the influence of radiotherapy on IL-2 and IL-6 secretions of mucous epithelial cells of small intestine and the inhibition effect of deproteinized calf blood extractive (DCBE, also known as Actovegin in trade name) on apoptosis of mucous epithelial cells of small intestine. 50 wistars were randomly divided into 5 groups with 10 in each including normal group (NG), radiation group (RG), low-dose Actovegin group (L-AG), middle-dose Actovegin group (M-AG), and high-dose Actovegin (H-AG). High-energy X-ray linear accelerator was used for abdominal irradiation of RG, L-AG, M-AG, and H-AG at the exposure dose of 9.0 Gy to establish the wistar radiation damage model. Modeling wistars were injected with medicine for successive 4 days, and their small intestinal mucosas were extracted as pathological sections; then fully automated analyzer was employed to detect their IL-2 and IL-6 levels. Immunohistochemical analysis was carried out to explore the effect of Actovegin on apoptosis of mucous membrane epithelial cells of small intestine. The IL-2 and IL-6 levels of RG are significantly higher than other groups and differences are statistically significant (P < 0.05); however, the IL-2 and IL-6 levels of L-AG, M-AG, and H-AG are higher than NG; there is no statistically significant difference between them (P > 0.05). Compared with RG, the villus height, membrane thickness, crypt depth, and whole layer thickness significantly improved (P < 0.05). However, the expression levels of apoptosis-related protein bax of M-AG and H-AG are significantly lower than RG, and their bcl-2 levels are higher than RG with significant difference between them (P < 0.05). Actovegin is capable of effectively inhibiting the expression of apoptosis-related protein bax and facilitating the expression of anti-apoptosis protein bcl-2, having preferable remediation effect on mucous membrane epithelial cells of radioactive enteritis.
authors: Department of Medical Oncology, Liaoning Cancer Hospital & Institute, 44 Xiaoheyan Road, Dadong District, Shenyang, 110042, China.
authors: Bin
authors: B
authors: Liu
authors: Xiaoling
authors: X
authors: Li
authors: Fulu
authors: F
authors: Ai
authors: Tianlu
authors: T
authors: Wang
authors: Yun
authors: Y
authors: Chen
authors: Hao
authors: H
authors: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000528:radiation effects; D007376:Interleukin-2 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology*; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D007376:Interleukin-2; D015850:Interleukin-6; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein