title: Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52.
abstract: The brown planthopper (BPH) is the most serious insect pest of rice in Asia. The indica rice cultivar ADR52 carries two BPH resistance genes, BPH26 (brown planthopper resistance 26) and BPH25. Map-based cloning of BPH26 revealed that BPH26 encodes a coiled-coil-nucleotide-binding-site-leucine-rich repeat (CC-NBS-LRR) protein. BPH26 mediated sucking inhibition in the phloem sieve element. BPH26 was identical to BPH2 on the basis of DNA sequence analysis and feeding ability of the BPH2-virulent biotype of BPH. BPH2 was widely incorporated in elite rice cultivars and was well-cultivated in many Asian countries as a favorable gene resource in rice breeding against BPH. However, BPH2 was rendered ineffective by a virulent biotype of BPH in rice fields in Asia. In this study, we suggest that BPH2 can be reused by combining with other BPH resistance genes, such as BPH25, to ensure durable resistance to BPH.
authors: Division of Insect Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8634, Japan.
authors: Yasumori
authors: Y
authors: Tamura
authors: Division of Insect Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8634, Japan.
authors: Makoto
authors: M
authors: Hattori
authors: Laboratory of Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi 464-8601, Japan.
authors: Hirofumi
authors: H
authors: Yoshioka
authors: Laboratory of Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University, Aichi 464-8601, Japan.
authors: Miki
authors: M
authors: Yoshioka
authors: Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan.
authors: Akira
authors: A
authors: Takahashi
authors: Agrogenomics Research Center, National Institute of Agrobiological Sciences, Ibaraki 305-8634, Japan.
authors: Jianzhong
authors: J
authors: Wu
authors: Division of Plant Sciences, National Institute of Agrobiological Sciences, Ibaraki 305-8604, Japan.
authors: Naoki
authors: N
authors: Sentoku
authors: Plant Breeding Laboratory, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
authors: Hideshi
authors: H
authors: Yasui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D006430:Hemiptera / Q000502:physiology; D007300:Insect Control; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism
chemical_list: D010940:Plant Proteins