title: Resveratrol inhibits breast cancer stem-like cells and induces autophagy via suppressing Wnt/β-catenin signaling pathway.
abstract: Resveratrol, a natural polyphenolic compound, is abundantly found in plant foods and has been extensively studied for its anti-cancer properties. Given the important role of CSCs (Cancer Stem Cells) in breast tumorigenesis and progression, it is worth investigating the effects of resveratrol on CSCs. The article is an attempt to investigate the effects of resveratrol on breast CSCs. Resveratrol significantly inhibits the proliferation of BCSCs (breast cancer stem-like cells) isolated from MCF-7 and SUM159, and decreased the percentage of BCSCs population, consequently reduced the size and number of mammospheres in non-adherent spherical clusters. Accordingly, the injection of resveratrol (100 mg/kg/d) in NOD/SCID (nonobese diabetic/severe combined immunodeficient) mice effectively inhibited the growth of xenograft tumors and reduced BCSC population in tumor cells. After the reimplantation of primary tumor cells into the secondary mice for 30 d, all 6 control inoculations produced tumors, while tumor cells derived from resveratrol-treated mice only caused 1 tumor of 6 inoculations. Further studies by TEM (Transmission electron microscopy) analysis, GFP-LC3-II puncta formation assay and western blot for LC3-II, Beclin1 and Atg 7, showed that resveratrol induces autophagy in BCSCs. Moreover, resveratrol suppresses Wnt/β-catenin signaling pathway in BCSCs; over-expression of β-catenin by transfecting the plasmid markedly reduced resveratrol-induced cytotoxicity and autophagy in BCSCs. Our findings indicated that resveratrol inhibits BCSCs and induces autophagy via suppressing Wnt/β-catenin signaling pathway.
authors: Research Center for Nutrition and Food Safety, Third Military Medical University; Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing, China; Institute of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, China.
authors: Yujie
authors: Y
authors: Fu
authors: Research Center for Nutrition and Food Safety, Third Military Medical University; Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing, China.
authors: Hui
authors: H
authors: Chang
authors: Research Center for Nutrition and Food Safety, Third Military Medical University; Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing, China; Department of Public Health, School of Preclinical Medicine, Chengdu Medical College, Chengdu, China.
authors: Xiaoli
authors: X
authors: Peng
authors: Research Center for Nutrition and Food Safety, Third Military Medical University; Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing, China.
authors: Qian
authors: Q
authors: Bai
authors: Research Center for Nutrition and Food Safety, Third Military Medical University; Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing, China.
authors: Long
authors: L
authors: Yi
authors: Research Center for Nutrition and Food Safety, Third Military Medical University; Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing, China.
authors: Yong
authors: Y
authors: Zhou
authors: Research Center for Nutrition and Food Safety, Third Military Medical University; Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing, China.
authors: Jundong
authors: J
authors: Zhu
authors: Research Center for Nutrition and Food Safety, Third Military Medical University; Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing, China.
authors: Mantian
authors: M
authors: Mi
mesh_terms: D001343:Autophagy / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D013267:Stilbenes; D051153:Wnt Proteins; D051176:beta Catenin; D000077185:Resveratrol