title: Longitudinal optical density analysis of subretinal fluid after surgical repair of rhegmatogenous retinal detachment.
abstract: OBJECTIVE
  To investigate optical coherence tomography-derived reflectivity and optical density (OD) characteristics of persistent subretinal fluid (SRF) in eyes after surgical repair of macula-off rhegmatogenous retinal detachment.
  
  
  METHODS
  Retrospective case series of nine eyes with macula-off rhegmatogenous retinal detachment that underwent surgical repair with either scleral buckling or vitrectomy with or without scleral buckling. Major inclusion criteria included 1) availability of high-quality optical coherence tomography scans at 2 or more time points, and 2) sufficient SRF for optical coherence tomography sampling without including tissue edges. Demographic, clinical, and optical coherence tomography imaging data were collected on all eyes. Optical density and SRF height measurements were obtained using a manual image segmentation method with ImageJ. Optical density measurements were standardized by conversion to optical density ratios to facilitate comparison between different visits and eyes. Correlations were assessed for significance through both univariate and multivariate regression analyses.
  
  
  RESULTS
  Optical density ratio measurements increased with time after surgery, and this was statistically significant (P = 0.001, R = 0.331). Subretinal fluid height measurements decreased in all eyes. There was a significant correlation between optical density ratios and log of SRF height (P ≤ 0.001, R = 0.485). In multivariate analysis, neither optical density ratios nor SRF height was a statistically significant predictor of visual acuity.
  
  
  CONCLUSIONS
  Changes in optical density ratios of the residual SRF after retinal detachment repair may be representative of changes in the SRF composition over time. This is in agreement with previous biochemical studies and may serve as a noninvasive method of assessing SRF content in vivo.
authors: *Department of Ophthalmology, University of Southern California, Los Angeles, California; †Department of Ophthalmology, Oakland University William Beaumont School of Medicine, Royal Oak, Michigan; and ‡Department of Vitreoretinal Surgery, Oakland University William Beaumont School of Medicine, Associated Retinal Consultants PC, Royal Oak, Michigan.
authors: Amir H
authors: AH
authors: Kashani
authors: Albert Y
authors: AY
authors: Cheung
authors: Joshua
authors: J
authors: Robinson
authors: George A
authors: GA
authors: Williams
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003720:Densitometry; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D012163:Retinal Detachment / Q000175:diagnosis / Q000503:physiopathology / Q000601:surgery*; D012189:Retrospective Studies; D012591:Scleral Buckling; D058471:Subretinal Fluid / Q000737:chemistry*; D041623:Tomography, Optical Coherence; D014792:Visual Acuity / Q000502:physiology; D014821:Vitrectomy