title: Zinc-chelated Vitamin C Stimulates Adipogenesis of 3T3-L1 Cells.
abstract: Adipose tissue development and function play a critical role in the regulation of energy balance, lipid metabolism, and the pathophysiology of metabolic syndromes. Although the effect of zinc ascorbate supplementation in diabetes or glycemic control is known in humans, the underlying mechanism is not well described. Here, we investigated the effect of a zinc-chelated vitamin C (ZnC) compound on the adipogenic differentiation of 3T3-L1 preadipocytes. Treatment with ZnC for 8 d significantly promoted adipogenesis, which was characterized by increased glycerol-3-phosphate dehydrogenase activity and intracellular lipid accumulation in 3T3-L1 cells. Meanwhile, ZnC induced a pronounced up-regulation of the expression of glucose transporter type 4 (GLUT4) and the adipocyte-specific gene adipocyte protein 2 (aP2). Analysis of mRNA and protein levels further showed that ZnC increased the sequential expression of peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα), the key transcription factors of adipogenesis. These results indicate that ZnC could promote adipogenesis through PPARγ and C/EBPα, which act synergistically for the expression of aP2 and GLUT4, leading to the generation of insulin-responsive adipocytes and can thereby be useful as a novel therapeutic agent for the management of diabetes and related metabolic disorders.
authors: Animal Environment Division, National Institute of Animal Science, RDA, Gyeonggi 441-706, Korea.
authors: Chiranjit
authors: C
authors: Ghosh
authors: Animal Environment Division, National Institute of Animal Science, RDA, Gyeonggi 441-706, Korea.
authors: Seung Hak
authors: SH
authors: Yang
authors: Animal Environment Division, National Institute of Animal Science, RDA, Gyeonggi 441-706, Korea.
authors: Jong Geun
authors: JG
authors: Kim
authors: Animal Environment Division, National Institute of Animal Science, RDA, Gyeonggi 441-706, Korea.
authors: Tae-Il
authors: TI
authors: Jeon
authors: Animal Environment Division, National Institute of Animal Science, RDA, Gyeonggi 441-706, Korea.
authors: Byung Hyun
authors: BH
authors: Yoon
authors: Animal Environment Division, National Institute of Animal Science, RDA, Gyeonggi 441-706, Korea.
authors: Jai Young
authors: JY
authors: Lee
authors: Animal Environment Division, National Institute of Animal Science, RDA, Gyeonggi 441-706, Korea.
authors: Eun Young
authors: EY
authors: Lee
authors: Animal Environment Division, National Institute of Animal Science, RDA, Gyeonggi 441-706, Korea.
authors: Seok Geun
authors: SG
authors: Choi
authors: Animal Environment Division, National Institute of Animal Science, RDA, Gyeonggi 441-706, Korea.
authors: Seong Gu
authors: SG
authors: Hwang
keywords: 3T3-L1; Adipogenesis; C/EBPα; GLUT4; PPARγ; Zinc-chelated Vitamin C