title: [Algorithm of toxigenic genetically altered Vibrio cholerae El Tor biovar strain identification].
abstract: OBJECTIVE
  Development of an algorithm of genetically altered Vibrio cholerae biovar El Tor strai identification that ensures determination of serogroup, serovar and biovar of the studied isolate based on pheno- and genotypic properties, detection of genetically altered cholera El Tor causative agents, their differentiation by epidemic potential as well as evaluation of variability of key pathogenicity genes.
  
  
  METHODS
  Complex analysis of 28 natural V. cholerae strains was carried out by using traditional microbiological methods, PCR and fragmentary sequencing.
  
  
  RESULTS
  An algorithm of toxigenic genetically altered V. cholerae biovar El Tor strain identification was developed that includes 4 stages: determination of serogroup, serovar and biovar based on phenotypic properties, confirmation of serogroup and biovar based on molecular-genetic properties determination of strains as genetically altered, differentiation of genetically altered strains by their epidemic potential and detection of ctxB and tcpA key pathogenicity gene polymorphism. The algorithm is based on the use of traditional microbiological methods, PCR and sequencing of gene fragments.
  
  
  CONCLUSIONS
  The use of the developed algorithm will increase the effectiveness of detection of genetically altered variants of the cholera El Tor causative agent, their differentiation by epidemic potential and will ensure establishment of polymorphism of genes that code key pathogenicity factors for determination of origins of the strains and possible routes of introduction of the infection.
authors: N I
authors: NI
authors: Smirnova
authors: D A
authors: DA
authors: Agafonov
authors: S P
authors: SP
authors: Zadnova
authors: A V
authors: AV
authors: Cherkasov
authors: V V
authors: VV
authors: Kutyrev
mesh_terms: D000465:Algorithms; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D036781:Fimbriae Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D012426:Russia; D017422:Sequence Analysis, DNA; D012703:Serotyping / Q000379:methods*; D014734:Vibrio cholerae / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D003470:Culture Media; C085807:TcpA protein, Vibrio cholerae; D036781:Fimbriae Proteins