title: Biological activities of heparan sulfate.
abstract: Heparan sulfate was isolated from two bivalve mollusks such as Tridacna maxima and Perna viridis. The isolated heparin was quantified in crude as well as purified samples and they were estimated as 2.72 and 2.2g/kg (crude) and 260 and 248 mg/g (purified) in T. maxima and P. viridis, respectively. Both the bivalves showed the anticoagulant activity of the crude and purified sample as 20,128 USP units/kg and 7.4 USP units/mg, 39,000 USP units/kg and 75 USP units/mg, 9460 USP units/kg and 4.3 USP units/mg, and 13,392 USP units/kg and 54 USP units/mg correspondingly in T. maxima and P. viridis. The antiproliferative activity that was studied with pulmonary artery smooth muscle cells using RPMI media reported that the result is in a dose-dependent manner. Among the two clams, P. viridis showed more antiproliferative activity than that of T. maxima.
authors: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, India. Electronic address: mamnplab@gmail.com.
authors: Muthuvel
authors: M
authors: Arumugam
authors: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, India.
authors: Sadhasivam
authors: S
authors: Giji
mesh_terms: D000818:Animals; D000925:Anticoagulants; D049872:Bivalvia / Q000737:chemistry*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006497:Heparan Sulfate / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D049883:Perna / Q000737:chemistry; D011651:Pulmonary Artery / Q000166:cytology
chemical_list: D000925:Anticoagulants; D006497:Heparan Sulfate
keywords: Antiproliferation; Giant clam; Glycosaminoglycans; Green mussel; Heparan sulfate; Heparin