title: Investigation of cytotoxicity of phosphoryl choline modified single-walled carbon nanotubes under a live cell station.
abstract: Single-walled carbon nanotubes (SWCNTs) and various modified SWCNTs have drawn a lot of attention due to their potential applications in biomedical field. Before further moving on to real clinical applications, hydrophobicity and toxicity of SWCNTs should be investigated thoroughly. In this paper, 2-methacryloyloxy ethyl phosphorylcholine (MPC) was adopted to modify SWCNTs and phosphoryl choline was grafted onto SWCNTs as small molecule moieties and polymeric chains, which made SWCNTs dispersed stably both in water and in cell culture medium for a long time. Cytotoxicity of pristine and modified SWCNTs were assayed upon successful preparation of the designed modified SWCNT. Furthermore, the internalization of SWCNTs by three cells was investigated using a live cell station under normal culture temperature (37°C) and low temperature (4°C). The results showed that the internalization of modified SWCNTs was related to both the active transport and the passive transport. Although the modification with phosphoryl choline remarkably reduced the cytotoxicity of SWCNTs, the results were probably due to other reasons such as the decrease in the ratio of cells which internalized modified SWCNTs since the cells without SWCNTs occupation still exhibited normal states.
authors: College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China.
authors: Yufeng
authors: Y
authors: Zhao
authors: College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China.
authors: Qunlong
authors: Q
authors: Mao
authors: College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China.
authors: Yu
authors: Y
authors: Liu
authors: College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China ; Nanjing Excellence Technology Center for Interventional Medical Devices, Nanjing 210093, China.
authors: Yan
authors: Y
authors: Zhang
authors: College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China ; Nanjing Excellence Technology Center for Interventional Medical Devices, Nanjing 210093, China.
authors: Tao
authors: T
authors: Zhang
authors: Physics Department, Nanjing University, Nanjing 210093, China.
authors: Zhengsheng
authors: Z
authors: Jiang
mesh_terms: D000818:Animals; D002470:Cell Survival; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry*; D015398:Signal Transduction; D013059:Spectrum Analysis, Raman; D013696:Temperature; D013818:Thermogravimetry
chemical_list: D008689:Methacrylates; D037742:Nanotubes, Carbon; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine