title: Profenofos insecticide degradation by novel microbial consortium and isolates enriched from contaminated chili farm soil.
abstract: Profenofos (PF) is one of the heavily used organophosphorus pesticides (OPPs) of which its contamination is ubiquitous in an agricultural area. This study aims to acquire and characterize PF-degrading bacterial cultures from contaminated soil. OPP degradation by the novel isolates was then investigated. The experiment was performed at the initial PF concentration of 20 mg/L. The result showed that the enriched consortium comprised three predominant PF-degrading strains designated as PF1, PF2, and PF3. The isolates (PF1, PF2, and PF3) were characterized as Pseudomonas plecoglossicida, Pseudomonas aeruginosa, and P. aeruginosa, respectively. A consortium and all isolates could utilize PF as a sole carbon source with PF removal of more than 90% via a hydrolysis process. The bacterial growth and PF degradation rates followed the first-order kinetic reaction with the rates of 0.4 to 2.7/h and 0.15 to 1.96/h, respectively. Additional carbon supplement deteriorated PF biodegradation. The enriched cultures were also capable for degrading chlorpyrifos and dicrotophos pesticides (33-73% removal). The results indicated that the consortium and isolates are efficient for PF and other OPP degradation and have potential for PF remediation.
authors: Department of Environmental Engineering, Faculty of Engineering, Khon Kaen University, 123 Mittapap Road, Muang District, Khon Kaen, 40002, Thailand, jeans_sumana@yahoo.com.
authors: Sumana
authors: S
authors: Siripattanakul-Ratpukdi
authors: Alisa S
authors: AS
authors: Vangnai
authors: Puttaporn
authors: P
authors: Sangthean
authors: Sirinuttakan
authors: S
authors: Singkibut
mesh_terms: D000383:Agriculture; D001673:Biodegradation, Environmental; D002212:Capsicum; D004390:Chlorpyrifos / Q000378:metabolism; D007306:Insecticides / Q000378:metabolism*; D059013:Microbial Consortia; D009943:Organophosphorus Compounds / Q000378:metabolism; D063086:Organothiophosphates / Q000378:metabolism*; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D007306:Insecticides; D009943:Organophosphorus Compounds; D063086:Organothiophosphates; D012989:Soil Pollutants; C024273:profenofos; C000944:dicrotophos; D004390:Chlorpyrifos