title: In vivo stable isotope labeling by amino acids in Drosophila melanogaster.
abstract: The fruit fly Drosophila melanogaster is one of the most widely used and well-studied model organisms in biology and therefore a promising tool for quantitative proteomics. Here, we describe a method to label D. melanogaster with stable isotope labeled amino acids in vivo. Feeding flies with heavy lysine labeled yeast cells leads to virtually complete heavy labeling already in the first filial generation. The approach is simple, fast, and cost-effective, which makes SILAC flies an attractive model system for the emerging field of in vivo quantitative proteomics.
authors: Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, Berlin, D-13092, Germany.
authors: Matthias D
authors: MD
authors: Sury
authors: Jia-Xuan
authors: JX
authors: Chen
authors: Matthias
authors: M
authors: Selbach
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D019476:Insect Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism
chemical_list: D000596:Amino Acids; D019476:Insect Proteins