title: Glycomyces artemisiae sp. nov., an endophytic actinomycete isolated from the roots of Artemisia argyi.
abstract: An endophytic actinomycete strain, IXS4(T), was isolated from the root of Artemisia argyi, a medicinal plant collected from Yesanpo located in Laishui county, Hebei province, China. The 16S rRNA gene sequence of strain IXS(T) showed most similarity to Glycomyces mayteni YIM 61331(T) (98.23% 16S rRNA gene sequence similarity), Glycomyces scopariae YIM 56256(T) (98.00%), Glycomyces sambucus E71(T) (97.90%) and Glycomyces algeriensis NRRL B-16327(T) (97.10%). DNA-DNA hybridization values between strain IXS4(T) and the closely related type strains were well below 70%. The strain also showed a number of physiological and biochemical characteristics that were distinct from the closely related species. The strain contained MK-10(H2) and MK-11(H0) as the detected menaquinones. The peptidoglycan was mainly meso-diaminopimelic acid and the whole-cell sugars contained galactose, glucose, mannose, xylose and ribose. The major cellular fatty acids were iso-C14:0, iso-C15:0, iso-C16:0, anteiso-C15:0 and anteiso-C17:0. Based on the genetic and phenotypic properties, it is proposed that strain IXS4(T) represents a novel species of the genus Glycomyces, with the name http://dx.doi.org/10.1601/nm.7671Glycomyces artemisiae sp. nov. The type strain is IXS4(T) ( = HBUM178000(T) = CGMCC 4.7067(T) = NBRC 109773(T)).
authors: Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, PR China.
authors: Xiumin
authors: X
authors: Zhang
authors: Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, PR China.
authors: Kai
authors: K
authors: Ren
authors: Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, PR China.
authors: Jiao
authors: J
authors: Du
authors: Central laboratory, No. 252 Hospital of PLA, Baoding, PR China.
authors: Haiyan
authors: H
authors: Liu
authors: Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, PR China.
authors: Liping
authors: L
authors: Zhang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019306:Artemisia / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid