title: Probing the epigenetic status at Notch target genes.
abstract: Chromatin-based mechanisms significantly contribute to the regulation of many developmentally regulated genes, including Notch target genes. After specific ligand binding, the intracellular part of the Notch receptor is cleaved off and translocates to the nucleus, where it binds to the transcription factor CSL (encoded by the RBPJ gene in mammals), in order to activate transcription. In the absence of a Notch signal, CSL represses Notch target genes by recruiting a co-repressor complex. Both NICD co-activator and CSL co-repressor complexes contain chromatin modifiers such as histone acetyltransferases and methyltransferases, which dynamically regulate chromatin marks at Notch target genes. Here we provide protocols for ChIP (chromatin immunoprecipitation) to analyze the chromatin status of dynamically regulated Notch target genes. Furthermore, an example is presented how to perform a primary analysis of ChIP-Seq data at Notch target genes using the Cistrome platform.
authors: Cell Biology Department, Harvard Medical School and Division of Newborn Medicine, Boston Children's Hospital, Boston, MA, USA.
authors: Robert
authors: R
authors: Liefke
authors: Tilman
authors: T
authors: Borggrefe
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D019295:Computational Biology / Q000379:methods; D044127:Epigenesis, Genetic; D006801:Humans; D051880:Receptors, Notch / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D002843:Chromatin; D051880:Receptors, Notch