title: Hsp74, a potential bladder cancer marker, has direct interaction with keratin 1.
abstract: Early diagnosis and prognosis monitoring are very important for the survival of patients with bladder cancer. To identify candidate biomarkers of bladder cancer, we used a combination of techniques including 2-DE, co-IP, western blot, LC-MS/MS, and immunohistochemistry. Hsp74 was identified with high expression in bladder cancer. The cellular location of expression products of gene Hsp74 showed that they were distributed into cytoplasm and keratin 1 was found to be associated with Hsp74. The results provide a new idea to understand the molecular basis of bladder cancer progression and pinpoint new potential molecular target for early diagnosis and therapeutic monitoring of bladder cancer.
authors: Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors: Ling
authors: L
authors: Chen
authors: Department of Radiology, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
authors: YaRong
authors: Y
authors: Wang
authors: Translational Medicine Center, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors: Le
authors: L
authors: Zhao
authors: Translational Medicine Center, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors: Wei
authors: W
authors: Chen
authors: Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors: Chunhui
authors: C
authors: Dong
authors: Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors: Xinhan
authors: X
authors: Zhao
authors: Translational Medicine Center, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors: Xu
authors: X
authors: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053556:Keratin-1 / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins; D011485:Protein Binding; D021381:Protein Transport; D040901:Proteomics / Q000379:methods; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D018840:HSP70 Heat-Shock Proteins; C492001:HSPA9 protein, human; D053556:Keratin-1; D024101:Mitochondrial Proteins