title: Genomic reassortants of pandemic A (H1N1) 2009 virus and endemic porcine H1 and H3 viruses in swine in Japan.
abstract: From 2010 to 2013 in Japan, we isolated 11 swine influenza viruses (SIVs) from pigs showing respiratory symptoms. Sequence and phylogenetic analyses showed that 6 H1N1 viruses originated from the pandemic (H1N1) 2009 (pdm 09) virus and the other 5 viruses were reassortants between SIVs and pdm 09 viruses, representing 4 genotypes. Two H1N2 viruses contained H1 and N2 genes originated from Japanese H1N2 SIV together with internal genes of pdm 09 viruses. Additionally, 1 H1N2 virus contained a further NP gene originating from Japanese H1N2 SIV. One H1N1 virus contained only the H1 gene originating from Japanese H1 SIV in a pdm 09 virus background. One H3N2 virus contained H3 and N2 genes originating from Japanese H3N2 SIV together with internal genes of pdm 09 virus. The results indicate that pdm 09 viruses are distributed widely in the Japanese swine population and that several reassortments with Japanese SIVs have occurred.
authors: Laboratory of Veterinary Virology, Department of Pathobiology, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyoudai Midori-machi, Ebetsu, Hokkaido 069-8501, Japan.
authors: Rikio
authors: R
authors: Kirisawa
authors: Yoshitaka
authors: Y
authors: Ogasawara
authors: Hayato
authors: H
authors: Yoshitake
authors: Asuka
authors: A
authors: Koda
authors: Tokujiro
authors: T
authors: Furuya
mesh_terms: D000818:Animals; D001483:Base Sequence; D004285:Dogs; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D049674:History, 21st Century; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D057221:Influenza A Virus, H1N2 Subtype / Q000235:genetics; D007564:Japan / Q000453:epidemiology; D061985:Madin Darby Canine Kidney Cells; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D058873:Pandemics / Q000266:history*; D016865:Reassortant Viruses / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology*