title: Effects of different dietary intake on mRNA levels of MSTN, IGF-I, and IGF-II in the skeletal muscle of Dorper and Hu sheep hybrid F1 rams.
abstract: MSTN, IGF-І(insulin-like growth factor-І) and IGF-II (insulin-like growth factor-II) regulate skeletal muscle growth. This study investigated the effects of different dietary intake levels on skeletal muscles. Sheep was randomly assigned to 3 feeding groups: 1) the maintenance diet (M), 2) 1.4 x the maintenance diet (1.4M), and 3) 2.15 x the maintenance diet (2.15M). Before slaughtering the animals, blood samples were collected to measure plasma urea, growth hormone, and insulin concentrations. After slaughtering, the longissimus dorsi, semitendinosus, semimembranosus, gastrocnemius, soleus, and chest muscle were removed to record various parameters, including the mRNA expression levels of MSTN and IGFs, in addition to skeletal muscle fiber diameter and cross-sectional area. The result showed that as dietary intake improved, the mRNA expression levels of MSTN and IGF-II decreased, whereas IGF-Іexpression increased. The mRNA expression levels of MSTN and IGFs were significantly different in the same skeletal muscle under different dietary intake. The skeletal muscle fiber diameter and cross-sectional area increased with greater dietary intake, as observed for the mRNA expression of IGF-І; however, it contrasted to that observed for the mRNA expression of MSTN and IGF-II. In conclusion, dietary intake levels have a certain influence on MSTN and IGFs mRNA expression levels, in addition to skeletal muscle fiber diameter and cross-sectional area. This study contributes valuable information for enhancing the molecular-based breeding of sheep.
authors: Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: H J
authors: HJ
authors: Xing
authors: Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: Z Y
authors: ZY
authors: Wang
authors: Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: B S
authors: BS
authors: Zhong
authors: Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: S J
authors: SJ
authors: Ying
authors: Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: H T
authors: HT
authors: Nie
authors: Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: Z R
authors: ZR
authors: Zhou
authors: Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: Y X
authors: YX
authors: Fan
authors: Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China caeet@njau.edu.cn.
authors: F
authors: F
authors: Wang
mesh_terms: D000821:Animal Feed; D000818:Animals; D001947:Breeding; D002678:Chimera / Q000235:genetics; D004032:Diet / Q000379:methods / Q000662:veterinary*; D005786:Gene Expression Regulation; D013006:Growth Hormone / Q000097:blood; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D055435:Myostatin / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D034561:Sheep, Domestic; D014508:Urea / Q000097:blood
chemical_list: D007328:Insulin; D055435:Myostatin; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D014508:Urea; D013006:Growth Hormone