title: Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain.
abstract: Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5'-promoter region which is 1221 bp in length. Real-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization exhibited the CHY-like reactivity presented in resorptive cells of the hepatopancreas. After bacterial challenge with Vibrio alginolyticus, the expression level of SpCHY mRNA was extremely up-regulated at 3 h in hepatopancreas. Our results suggest that SpCHY might play an important role in the mud crab's immune response.
authors: College of Ocean and Earth Sciences , Xiamen University , Xiamen, Fujian , China .
authors: Jie
authors: J
authors: Gong
authors: College of Ocean and Earth Sciences , Xiamen University , Xiamen, Fujian , China .
authors: Yinjie
authors: Y
authors: Xie
authors: College of Ocean and Earth Sciences , Xiamen University , Xiamen, Fujian , China .
authors: Kun
authors: K
authors: Yu
authors: College of Ocean and Earth Sciences , Xiamen University , Xiamen, Fujian , China .
authors: Ya'nan
authors: Y
authors: Yang
authors: College of Ocean and Earth Sciences , Xiamen University , Xiamen, Fujian , China .
authors: Huiyang
authors: H
authors: Huang
authors: College of Ocean and Earth Sciences , Xiamen University , Xiamen, Fujian , China . ; Center for Marine Biotechnology , Xiamen University , Xiamen, Fujian , China .
authors: Haihui
authors: H
authors: Ye
keywords: Scylla paramamosain; chymotrypsin; immune response; immunocytochemistry; in situ hybridization