title: Inorganic-organic hybrid nanoprobe for NIR-excited imaging of hydrogen sulfide in cell cultures and inflammation in a mouse model.
abstract: Hydrogen sulfide (H2S) is an important gaseous signaling agent mediated by many physiological processes and diseases. In order to explore its role in biological signaling, much effort has been focused on developing organic fluorescent probes to image H2S. However, these downconversion H2S probes are impractical for bio-imaging beyond a certain depth because of the short tissue penetration of UV/visible light (as an excitation source). In most circumstance, these probes are also not suitable for long-term assay due to photo-bleaching. Herein, a new design to detect H2S based on the coumarin-hemicyanine (CHC1)-modified upconversion nanophosphors is reported. This inorganic-organic integrated nanoprobe is demonstrated to display a fast response time with a large ratiometric upconversion luminescence (UCL) enhancement, and extraordinary photo-stability. CHC1-UCNPs not only can be used for ratiometric UCL monitoring of pseudo-enzymatic H2S production in living cells, but can also be used to identify the risk of endotoxic shock through ratiometric UCL imaging of tissue and measurement of endogenous H2S levels in plasma. The first ratiometric UCL H2S nanoprobe reported here may be further developed as the next-generation diagnostic tool for the detection of inflammatory-related diseases.
authors: School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
authors: Yi
authors: Y
authors: Zhou
authors: Wangqiao
authors: W
authors: Chen
authors: Jixin
authors: J
authors: Zhu
authors: Wenbo
authors: W
authors: Pei
authors: Chengyuan
authors: C
authors: Wang
authors: Ling
authors: L
authors: Huang
authors: Cheng
authors: C
authors: Yao
authors: Qinyu
authors: Q
authors: Yan
authors: Wei
authors: W
authors: Huang
authors: Joachim Say Chye
authors: JS
authors: Loo
authors: Qichun
authors: Q
authors: Zhang
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry; D003374:Coumarins / Q000737:chemistry; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry*; D007249:Inflammation; D008070:Lipopolysaccharides / Q000737:chemistry; D049449:Luminescence; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D012772:Shock, Septic / Q000097:blood / Q000175:diagnosis; D015398:Signal Transduction; D013056:Spectrophotometry, Ultraviolet; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D002232:Carbocyanines; D003374:Coumarins; D005456:Fluorescent Dyes; D008070:Lipopolysaccharides; C000601156:tetramethylene hemicyanine; C030123:coumarin; D006862:Hydrogen Sulfide
keywords: NIR-Excited imaging; hydrogen sulfide; ratiometric; signaling molecule; upconversion luminescence