title: Comparative analysis of H&E and Prussian blue staining in a mouse model of cerebral microbleeds.
abstract: Cerebral microbleeds are microscopic hemorrhages with deposits of blood products in the brain, which can be visualized with MRI and are implicated in cerebrovascular diseases. Hematoxylin and eosin (H&E) and Perl's Prussian blue are popular staining methods used to localize cerebral microbleeds in pathology. This paper compared these two staining techniques in a mouse model of cerebral microbleeds. We used lipopolysaccharide (LPS) to induce cerebral microhemorrhages. C57B6 mice were treated with LPS (5 mg/kg, i.p.) or vehicle at baseline and at 24 hr. The brains were extracted 48 hr after the first injection and adjacent coronal sections were stained with H&E and Prussian blue to compare the effectiveness of the two staining techniques. H&E-positive stains were increased with LPS treatment and were correlated with grossly visible microhemorrhages on the brain surface; Prussian blue-positive stains, by comparison, showed no significant increase with LPS treatment and did not correlate with either H&E-positive stains or surface microhemorrhages. H&E staining is thus a more reliable indicator of acute bleeding events induced by LPS in this model within a short time span.
authors: Department of Anatomy and Neurobiology (SL, MJF), University of California, Irvine, CaliforniaDepartment of Neurology (MMG, RKS, APH, MJF), University of California, Irvine, CaliforniaInstitute for Memory Impairments and Neurological Disorders (VV, DHC), University of California, Irvine, CaliforniaKeck Graduate Institute, School of Pharmacy, Claremont, California (RKS), University of California, Irvine, CaliforniaDepartment of Pathology and Laboratory Medicine (MJF), University of California, Irvine, California.
authors: Shuo
authors: S
authors: Liu
authors: Department of Anatomy and Neurobiology (SL, MJF), University of California, Irvine, CaliforniaDepartment of Neurology (MMG, RKS, APH, MJF), University of California, Irvine, CaliforniaInstitute for Memory Impairments and Neurological Disorders (VV, DHC), University of California, Irvine, CaliforniaKeck Graduate Institute, School of Pharmacy, Claremont, California (RKS), University of California, Irvine, CaliforniaDepartment of Pathology and Laboratory Medicine (MJF), University of California, Irvine, California.
authors: Mher Mahoney
authors: MM
authors: Grigoryan
authors: Department of Anatomy and Neurobiology (SL, MJF), University of California, Irvine, CaliforniaDepartment of Neurology (MMG, RKS, APH, MJF), University of California, Irvine, CaliforniaInstitute for Memory Impairments and Neurological Disorders (VV, DHC), University of California, Irvine, CaliforniaKeck Graduate Institute, School of Pharmacy, Claremont, California (RKS), University of California, Irvine, CaliforniaDepartment of Pathology and Laboratory Medicine (MJF), University of California, Irvine, California.
authors: Vitaly
authors: V
authors: Vasilevko
authors: Department of Anatomy and Neurobiology (SL, MJF), University of California, Irvine, CaliforniaDepartment of Neurology (MMG, RKS, APH, MJF), University of California, Irvine, CaliforniaInstitute for Memory Impairments and Neurological Disorders (VV, DHC), University of California, Irvine, CaliforniaKeck Graduate Institute, School of Pharmacy, Claremont, California (RKS), University of California, Irvine, CaliforniaDepartment of Pathology and Laboratory Medicine (MJF), University of California, Irvine, California.
authors: Rachita K
authors: RK
authors: Sumbria
authors: Department of Anatomy and Neurobiology (SL, MJF), University of California, Irvine, CaliforniaDepartment of Neurology (MMG, RKS, APH, MJF), University of California, Irvine, CaliforniaInstitute for Memory Impairments and Neurological Disorders (VV, DHC), University of California, Irvine, CaliforniaKeck Graduate Institute, School of Pharmacy, Claremont, California (RKS), University of California, Irvine, CaliforniaDepartment of Pathology and Laboratory Medicine (MJF), University of California, Irvine, California.
authors: Annlia
authors: A
authors: Paganini-Hill
authors: Department of Anatomy and Neurobiology (SL, MJF), University of California, Irvine, CaliforniaDepartment of Neurology (MMG, RKS, APH, MJF), University of California, Irvine, CaliforniaInstitute for Memory Impairments and Neurological Disorders (VV, DHC), University of California, Irvine, CaliforniaKeck Graduate Institute, School of Pharmacy, Claremont, California (RKS), University of California, Irvine, CaliforniaDepartment of Pathology and Laboratory Medicine (MJF), University of California, Irvine, California.
authors: David H
authors: DH
authors: Cribbs
authors: Department of Anatomy and Neurobiology (SL, MJF), University of California, Irvine, CaliforniaDepartment of Neurology (MMG, RKS, APH, MJF), University of California, Irvine, CaliforniaInstitute for Memory Impairments and Neurological Disorders (VV, DHC), University of California, Irvine, CaliforniaKeck Graduate Institute, School of Pharmacy, Claremont, California (RKS), University of California, Irvine, CaliforniaDepartment of Pathology and Laboratory Medicine (MJF), University of California, Irvine, California mfisher@uci.edu.
authors: Mark J
authors: MJ
authors: Fisher
mesh_terms: D000818:Animals; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000175:diagnosis*; D004195:Disease Models, Animal; D004801:Eosine Yellowish-(YS); D005295:Ferrocyanides; D006416:Hematoxylin; D008070:Lipopolysaccharides; D051379:Mice; D008810:Mice, Inbred C57BL; D013194:Staining and Labeling
chemical_list: D005295:Ferrocyanides; D008070:Lipopolysaccharides; D004801:Eosine Yellowish-(YS); C000170:ferric ferrocyanide; D006416:Hematoxylin
keywords: Cerebral microbleeds; H&E; LPS; Prussian Blue