title: Effects of age, nursing, and oral IGF1 supplementation on neonatal porcine cervical development.
abstract: Nursing supports neonatal porcine uterine and testicular development, however, lactocrine effects on cervical development are undefined. Studies were conducted to determine the effects of i) age and the imposition of the lactocrine-null state from birth (postnatal day 0 (PND0)) by milk replacer feeding on cervical histology; ii) imposition of the lactocrine-null state for 2 days from birth on cervical cell proliferation, as reflected by proliferating cell nuclear antigen immunostaining; and iii) a single feeding of colostrum or milk replacer, administered at birth, with or without oral IGF1, on cervical cell proliferation and phosphorylated AKT (pAKT) and B-cell lymphoma 2 (BCL2) protein levels at 12 h postnatal. Cervical crypt depth and height of luminal epithelium (LE) increased with age by PND14, when both responses were reduced in replacer-fed gilts. Cell proliferation was reduced in LE at PND2, and in crypt epithelium and stroma by PND14 in replacer-fed gilts. Returning replacer-fed gilts to nursing on PND2 did not rescue the cervical phenotype by PND14. A single feeding of colostrum, but not milk replacer, was sufficient to support cervical cell proliferation at 12 h postnatal. IGF1 supplementation induced cell proliferation in replacer-fed gilts, and increased cervical pAKT and BCL2 levels in colostrum-fed gilts and replacer-fed gilts at 12 h postnatal. Results indicate that age and nursing support porcine cervical development, support is initiated at first ingestion of colostrum, IGF1 may be lactocrine-active, and identification of lactocrine-active factors can be accomplished by 12 h postnatal using this bioassay system.
authors: Department of Animal SciencesEndocrinology and Animal Biosciences Program, Rutgers University, Foran Hall, 59 Dudley Road, New Brunswick, New Jersey 08901, USADepartment of AnatomyPhysiology and Pharmacology, Cellular and Molecular Biosciences Program, Auburn University, Auburn, Alabama 36849, USA.
authors: Meredith E
authors: ME
authors: Camp
authors: Department of Animal SciencesEndocrinology and Animal Biosciences Program, Rutgers University, Foran Hall, 59 Dudley Road, New Brunswick, New Jersey 08901, USADepartment of AnatomyPhysiology and Pharmacology, Cellular and Molecular Biosciences Program, Auburn University, Auburn, Alabama 36849, USA.
authors: Anne A
authors: AA
authors: Wiley
authors: Department of Animal SciencesEndocrinology and Animal Biosciences Program, Rutgers University, Foran Hall, 59 Dudley Road, New Brunswick, New Jersey 08901, USADepartment of AnatomyPhysiology and Pharmacology, Cellular and Molecular Biosciences Program, Auburn University, Auburn, Alabama 36849, USA.
authors: Monica B
authors: MB
authors: Boulos
authors: Department of Animal SciencesEndocrinology and Animal Biosciences Program, Rutgers University, Foran Hall, 59 Dudley Road, New Brunswick, New Jersey 08901, USADepartment of AnatomyPhysiology and Pharmacology, Cellular and Molecular Biosciences Program, Auburn University, Auburn, Alabama 36849, USA.
authors: Kathleen M
authors: KM
authors: Rahman
authors: Department of Animal SciencesEndocrinology and Animal Biosciences Program, Rutgers University, Foran Hall, 59 Dudley Road, New Brunswick, New Jersey 08901, USADepartment of AnatomyPhysiology and Pharmacology, Cellular and Molecular Biosciences Program, Auburn University, Auburn, Alabama 36849, USA.
authors: Frank F
authors: FF
authors: Bartol
authors: Department of Animal SciencesEndocrinology and Animal Biosciences Program, Rutgers University, Foran Hall, 59 Dudley Road, New Brunswick, New Jersey 08901, USADepartment of AnatomyPhysiology and Pharmacology, Cellular and Molecular Biosciences Program, Auburn University, Auburn, Alabama 36849, USA bagnell@aesop.rutgers.edu.
authors: Carol A
authors: CA
authors: Bagnell
mesh_terms: D000284:Administration, Oral; D000367:Age Factors; D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002584:Cervix Uteri / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D003126:Colostrum / Q000378:metabolism; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage*; D007774:Lactation; D041941:Milk Substitutes / Q000008:administration & dosage; D010641:Phenotype; D010766:Phosphorylation; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D034421:Sus scrofa
chemical_list: D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D007334:Insulin-Like Growth Factor I; D051057:Proto-Oncogene Proteins c-akt