title: GOLPH3, a good prognostic indicator in early-stage NSCLC related to tumor angiogenesis.
abstract: BACKGROUND
  Golgi phosphoprotein-3 (GOLPH3) is implicated in cancer development and progression. The aim of this study was to evaluate the prognostic significance of GOLPH3 protein and its association with tumor angiogenesis in patients with early-stage NSCLC.
  
  
  METHODS
  Immunohistochemistry was performed to determine GOLPH3 protein expression and allow assessment of intratumoral microvessel density (MVD) by counting CD-34 positive immunostained endothelial cells. Correlations of expression with MVD, clinicopathologic features and clinical prognosis were analyzed.
  
  
  RESULTS
  A notably higher level of GOLPH3 expression was found in early-stage NSCC tissues at the protein level. However, we do not find any correlation between GOLPH3 expression and clinicopathologic features (p>0.05), although higher MVD was positively associated with GOLPH3 overexpression (p<0.001). Expression of GOLPH3 was found to be an independent prognostic factor in early- stage NSCLC patients, those expressing high levels of GOLPH3 exhibiting a substantially lower 5-year overall survival than GOLPH3-negative patients (adjusted HR =1.899, 95% CI: 1.021-3.532, p=0.043).
  
  
  CONCLUSIONS
  High expression of the GOLPH3 protein is common in early-stage NSCC, and is closely associated with tumor progression, increased tumor angiogenesis, and poor survival. We conclude a possibility of its use as a diagnostic and prognostic marker in early-stage NSCC patients.
authors: Department of Thoracic Surgery, Qilu Hospital, Shandong University, Jinan, China E-mail : tianhuiql@126.com.
authors: Ming
authors: M
authors: Lu
authors: Yu
authors: Y
authors: Tian
authors: Wei-Ming
authors: WM
authors: Yue
authors: Lin
authors: L
authors: Li
authors: Shu-Hai
authors: SH
authors: Li
authors: Lei
authors: L
authors: Qi
authors: Wen-Si
authors: WS
authors: Hu
authors: Cun
authors: C
authors: Gao
authors: Li-Bo
authors: LB
authors: Si
authors: Hui
authors: H
authors: Tian
mesh_terms: D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D018450:Disease Progression; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D055806:Microvessels; D009389:Neovascularization, Pathologic / Q000235:genetics*; D011379:Prognosis; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D014408:Biomarkers, Tumor; C429024:GOLPH3 protein, human; D008565:Membrane Proteins; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases