title: A comparative study of BioAggregate and ProRoot MTA on adhesion, migration, and attachment of human dental pulp cells.
abstract: BACKGROUND
  The aim of the present study was to evaluate the effects of a novel bioceramic nanoparticular cement, BioAggregate (Innovative Bioceramix, Vancouver, BC, Canada), on the adhesion, migration, and attachment of human dental pulp cells (HDPCs) and to compare its performance with that of ProRoot mineral trioxide aggregate (MTA) (Dentsply, Tulsa, OK).
  
  
  METHODS
  Primary cultured HDPCs were treated with various dilutions of BioAggregate and MTA extracts to assess the cell viability using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan). Cell adhesion assay was performed using type I collagen-coated plates. An in vitro scratch wound healing model was used to determine cell migration. Focal adhesion formation and cytoskeleton organization were further examined by double immunofluorescence labeling for vinculin and fibrous actin. To assess cell attachment, HDPCs were directly seeded onto the material surfaces and observed by scanning electron microscopy.
  
  
  RESULTS
  HDPCs exposed to BioAggregate extracts showed the highest viabilities at all extract concentrations at 24 and 48 hours, whereas cells exposed to original MTA extracts displayed suppressed viabilities at 72 hours compared with the control. Treatment with BioAggregate extracts enhanced cellular adhesion and migration of HDPCs in a concentration-dependent manner, which was superior to the effects induced by MTA extracts. Immunofluorescence staining indicated that both BioAggregate and MTA optimized focal adhesion formation and stress fiber assembly. Furthermore, scanning electron microscopic analysis revealed that HDPCs attached onto BioAggregate were more flattened and exhibited better spreading than cells on MTA.
  
  
  CONCLUSIONS
  BioAggregate is able to promote cellular adhesion, migration, and attachment of HDPCs, indicating its excellent cytocompatibility. Therefore, BioAggregate appears to be a possible alternative to MTA for pulp capping.
authors: State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors: Lingxin
authors: L
authors: Zhu
authors: State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors: Jingwen
authors: J
authors: Yang
authors: State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors: Jie
authors: J
authors: Zhang
authors: State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China. Electronic address: phs301@vip.163.com.
authors: Bin
authors: B
authors: Peng
mesh_terms: D000199:Actins / Q000032:analysis; D000293:Adolescent; D000328:Adult; D017607:Aluminum Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D017610:Calcium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000008:administration & dosage / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry; D003599:Cytoskeleton / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D006801:Humans; D006882:Hydroxyapatites / Q000008:administration & dosage / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000008:administration & dosage / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors; D016596:Vinculin / Q000032:analysis; D055815:Young Adult
chemical_list: D000199:Actins; D017607:Aluminum Compounds; C557009:BioAggregate; D017610:Calcium Compounds; D024042:Collagen Type I; D004338:Drug Combinations; D006882:Hydroxyapatites; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D016596:Vinculin; D002126:Calcium Hydroxide
keywords: Adhesion; BioAggregate; attachment; human dental pulp cells; migration; mineral trioxide aggregate