title: Association between alterations in global DNA methylation and predisposing factors in diabetes: a high pressure liquid chromatography based study.
abstract: OBJECTIVE
  The aim of this study was to investigate the relationship between inter-individual global DNA methylation and diabetes predisposing factors.
  
  
  METHODS
  The 5-methyl cytosine content was assessed by reverse phase high pressure liquid chromatography (RP-HPLC) of peripheral blood leukocytes obtained from 178 type 2 diabetes patients to determine individual global DNA methylation status.
  
  
  RESULTS
  There was a positive significant correlation between diabetes duration and DNA methylation levels (P=0.002) with increasing levels of DNA methylation associated with age (P=0.047). There was no significant correlation between DNA methylation levels and HbA1c (P=0.15). No significant differences were observed between patients with and without diabetes predisposing factors including: hypertension (P=0.772), dyslipidemia (P=0.617), insulin resistance (homeostatic model assessment index) (P=0.156) and obesity (P=0.609). As such, the duration of diabetes (>10 years) was the most important predictor of global DNA methylation levels in diabetic patients after adjusting for age and sex (P=0.023).
  
  
  CONCLUSIONS
  Our findings indicate that chronic hyperglycemic exposure plays an independent role in global DNA methylation levels in type 2 diabetes patients.
authors: Endocrinology and Metabolism Research Center, Endocrinology and Metabolism,Clinical Sciences Institute, Tehran, University of Medical Sciences, Tehran, Iran - arash_hsi@yahoo.com.
authors: Z
authors: Z
authors: Maghbooli
authors: A
authors: A
authors: Hossein-Nezhad
authors: B
authors: B
authors: Larijani
authors: P
authors: P
authors: Pasalar
authors: A A
authors: AA
authors: Keshtkar
mesh_terms: D000419:Albuminuria / Q000652:urine; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D002851:Chromatography, High Pressure Liquid; D003430:Cross-Sectional Studies; D019175:DNA Methylation; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications / Q000175:diagnosis* / Q000652:urine; D018450:Disease Progression; D050171:Dyslipidemias / Q000150:complications; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D006973:Hypertension / Q000150:complications; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D006442:Glycated Hemoglobin