title: mRNA decay factor AUF1 binds the internal ribosomal entry site of enterovirus 71 and inhibits virus replication.
abstract: AU-rich element binding factor 1 (AUF1) has a role in the replication cycles of different viruses. Here we demonstrate that AUF1 binds the internal ribosome entry site (IRES) of enterovirus 71 (EV71) and negatively regulates IRES-dependent translation. During EV71 infection, AUF1 accumulates in the cytoplasm where viral replication occurs, whereas AUF1 localizes predominantly in the nucleus in mock-infected cells. AUF1 knockdown in infected cells increases IRES activity and synthesis of viral proteins. Taken together, the results suggest that AUF1 interacts with the EV71 IRES to negatively regulate viral translation and replication.
authors: School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.
authors: Jing-Yi
authors: JY
authors: Lin
authors: Department of Biochemistry & Molecular Biology, Rutgers Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America.
authors: Mei-Ling
authors: ML
authors: Li
authors: Department of Biochemistry & Molecular Biology, Rutgers Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America.
authors: Gary
authors: G
authors: Brewer
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D001667:Binding, Competitive; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D029821:Enterovirus A, Human / Q000502:physiology*; D004769:Enterovirus Infections / Q000378:metabolism* / Q000821:virology; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D / Q000378:metabolism*; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000378:metabolism; D055029:Inverted Repeat Sequences; D011485:Protein Binding; D014176:Protein Biosynthesis; D020871:RNA Stability; D012367:RNA, Viral / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014779:Virus Replication
chemical_list: D020121:5' Untranslated Regions; C000630986:HNRNPD protein, human; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D012367:RNA, Viral; D014764:Viral Proteins