title: Thermomonas carbonis sp. nov., isolated from the soil of a coal mine.
abstract: Strain GZ436(T) was Gram-stain-negative, aerobic, non-motile, rod-shaped and isolated from the soil of a coal mine. 16S rRNA gene phylogenetic analysis showed that this strain clustered with Thermomonas brevis LMG 21746(T) (97.5%), Thermomonas haemolytica A50-7-3(T) (96.3%), Thermomonas koreensis KCTC 12540(T) (96.4%), Thermomonas hydrothermalis SGM-6(T) (95.5%) and Thermomonas fusca LMG 21737(T) (95.1%). The major isoprenoid quinone was Q-8. The DNA G+C content was 67 mol%. Strain GZ436(T) contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unknown aminophospholipid, an unknown phospholipid and an unknown lipid as the major polar lipids. The predominant cellular fatty acids (>5%) were iso-C(15:0), iso-C(11:0), iso-C(11:0 3)-OH, iso-C(17:1)ω9c, C(16 : 0) and summed feature 3. The DNA-DNA relatedness value between strain GZ436(T) and T. brevis LMG 21746(T) was 54 ± 0.4%. According to phenotypic and phylogenetic characteristics, strain GZ436(T) represents a novel species of the genus Thermomonas, for which the name Thermomonas carbonis sp. nov. is proposed. The type strain is GZ436(T) ( =CCTCC AB 2013364(T) = KCTC 42013(T)).
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Liang
authors: L
authors: Wang
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Shixue
authors: S
authors: Zheng
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Dan
authors: D
authors: Wang
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Lu
authors: L
authors: Wang
authors: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors: Gejiao
authors: G
authors: Wang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D003031:Coal; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008906:Mining; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D003031:Coal; D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8