title: Post-mortem hemoparasite detection in free-living Brazilian brown brocket deer (Mazama gouazoubira, Fischer 1814).
abstract: Tick-borne infections can result in serious health problems for wild ruminants, and some of these infectious agents can be considered zoonosis. The aim of the present study was the post-mortem detection of hemoparasites in free-living Mazama gouazoubira from Minas Gerais state, Brazil. The deer samples consisted of free-living M. gouazoubira (n = 9) individuals that died after capture. Necropsy examinations of the carcasses were performed to search for macroscopic alterations. Organ samples were collected for subsequent imprint slides, and nested PCR assays were performed to detect hemoparasite species. Imprint slide assays from four deer showed erythrocytes infected with Piroplasmida small trophozoites, and A. marginale corpuscles were observed in erythrocytes from two animals. A. marginale and trophozoite co-infections occurred in two deer. A nested PCR analysis of the organs showed that six of the nine samples were positive for Theileria sp., five were positive for A. phagocytophilum and three were positive for A. marginale, with co-infection occurring in four deer. The results of the present study demonstrate that post-mortem diagnostics using imprint slides and molecular assays are an effective method for detecting hemoparasites in organs.
authors: Departamento de Parasitologia, Instituto de Ciências Biológicas ? ICB, Universidade Federal de Minas Gerais ? UFMG, Belo Horizonte, MG, Brasil.
authors: Júlia Angélica Gonçalves da
authors: JA
authors: Silveira
authors: Departamento de Parasitologia, Instituto de Ciências Biológicas ? ICB, Universidade Federal de Minas Gerais ? UFMG, Belo Horizonte, MG, Brasil.
authors: Elida Mara Leite
authors: EM
authors: Rabelo
authors: Departamento de Parasitologia, Instituto de Ciências Biológicas ? ICB, Universidade Federal de Minas Gerais ? UFMG, Belo Horizonte, MG, Brasil.
authors: Paula Cristina Senra
authors: PC
authors: Lima
authors: Departamento de Biologia Geral, Instituto de Ciências Biológicas ? ICB, Universidade Federal de Minas Gerais ? UFMG, Belo Horizonte, MG, Brasil.
authors: Bárbara Neves
authors: BN
authors: Chaves
authors: Departamento de Parasitologia, Instituto de Ciências Biológicas ? ICB, Universidade Federal de Minas Gerais ? UFMG, Belo Horizonte, MG, Brasil.
authors: Múcio Flávio Barbosa
authors: MF
authors: Ribeiro
mesh_terms: D000818:Animals; D001344:Autopsy; D001938:Brazil; D003670:Deer / Q000097:blood* / Q000469:parasitology*; D005260:Female; D008297:Male; D010273:Parasitic Diseases, Animal / Q000097:blood* / Q000175:diagnosis