title: Expression analysis and biological activity of moronecidin from rock bream, Oplegnathus fasciatus.
abstract: The piscidin-family, one of antimicrobial peptides (AMPs) mainly distributed in fish, is crucial effectors of fish innate immune response. Piscidin-family typically has broad-spectrum antimicrobial activity and the ability to modulate the immune response. In this study, we identified moronecidin (Rbmoro) included in piscidin-family from rock bream and investigated its gene expression using quantitative real-time PCR and biological activity (including antimicrobial and cytotoxic activity). The coding region of Rbmoro was 204 bp encoding 67 amino acid residues. Tertiary structure prediction of Rbmoro showed an amphipathic α-helical structure. Rbmoro gene was widely expressed in different tissues of healthy fish. Additionally, Rbmoro gene expression was induced in all tested tissues after infection with Edwardsiella tarda, Streptococcus iniae and red seabream iridovirus. We synthesized mature peptide of Rbmoro based on amino acid sequence of its AMP 12 domain, and the synthetic peptide appeared broad-spectrum antimicrobial activity to various bacteria. However, the synthetic peptide has weak haemolytic activity against fish erythrocytes. These results suggest that Rbmoro might play an important role in innate immune response of rock bream.
authors: Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea.
authors: Jin-Sol
authors: JS
authors: Bae
authors: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
authors: Sang Hee
authors: SH
authors: Shim
authors: Aquatic Life Disease Control Division, National Fisheries Research and Development Institute (NFRDI), 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors: Seong Don
authors: SD
authors: Hwang
authors: Aquatic Life Disease Control Division, National Fisheries Research and Development Institute (NFRDI), 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors: Myoung-Ae
authors: MA
authors: Park
authors: Aquatic Life Disease Control Division, National Fisheries Research and Development Institute (NFRDI), 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors: Bo-Young
authors: BY
authors: Jee
authors: Biotechnology Research Division, NFRDI, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors: Cheul-Min
authors: CM
authors: An
authors: Biotechnology Research Division, NFRDI, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors: Young-Ok
authors: YO
authors: Kim
authors: Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea.
authors: Ju-Won
authors: JW
authors: Kim
authors: Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea. Electronic address: vinus96@hanmail.net.
authors: Chan-Il
authors: CI
authors: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D004266:DNA Virus Infections / Q000276:immunology / Q000662:veterinary / Q000821:virology; D020609:Edwardsiella tarda / Q000502:physiology; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D007497:Iridoviridae / Q000502:physiology; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D013290:Streptococcal Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D013291:Streptococcus / Q000502:physiology
chemical_list: D023181:Antimicrobial Cationic Peptides; D029941:Fish Proteins; C449802:moronecidin protein, Morone saxatilis
keywords: Antimicrobial activity; Antimicrobial peptides; Cytotoxic activity; Moronecidin; Rock bream