title: NOD-Rag2null IL-2Rγnull mice: an alternative to NOG mice for generation of humanized mice.
abstract: We have developed NOD-Rag2(null) IL-2Rγ(null) (NR2G) mice similar to NOD-scidIL-2Rγ(null) (NOG) mice that are known as an excellent host to generate humanized mice. To evaluate the usefulness of NR2G mice as a host for humanized mice, the engraftment rates and differentiation of human cells after human hematopoietic stem cell (HSC) transplantation were compared among NR2G, NOG, and NOD-scid mice. For this purpose, the appropriate irradiation doses to expand the niche for human stem cells in the bone marrow were first determined. As a result, 8 and 2.5 Gy in adult, and 4 and 1 Gy in newborn NR2G and NOG mice, respectively, were found to be appropriate. Next, 5 × 10(4) human umbilical cord blood CD34(+) cells were intravenously inoculated into irradiated adult or newborn of the immunodeficient mice. These HSC transplantation experiments demonstrated that both NR2G and NOG mice showed high engraftment rates compared with NOD-scid mice, although NOG mice showed a slightly higher engraftment rate than that for NR2G mice. However, no difference was found in the human cell populations differentiated from HSCs between NR2G and NOG mice. The HSC transplantation experiments to adults and newborns of two immunodeficient mice also revealed that the HSC transplantation into newborn mice resulted in higher engraftment rate than those into adults. These results showed that NR2G mice could be used as an alternative host to NOG mice to generate humanized mice.
authors: Central Institute for Experimental Animals, 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 216-0001, Japan.
authors: Ikumi
authors: I
authors: Katano
authors: Ryoji
authors: R
authors: Ito
authors: Tsutomu
authors: T
authors: Kamisako
authors: Tomoo
authors: T
authors: Eto
authors: Tomoyuki
authors: T
authors: Ogura
authors: Kenji
authors: K
authors: Kawai
authors: Hiroshi
authors: H
authors: Suemizu
authors: Takeshi
authors: T
authors: Takahashi
authors: Yutaka
authors: Y
authors: Kawakami
authors: Mamoru
authors: M
authors: Ito
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018952:Antigens, CD34; D004268:DNA-Binding Proteins / Q000172:deficiency; D004195:Disease Models, Animal; D005312:Fetal Blood / Q000637:transplantation; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011829:Radiation Dosage; D015375:Receptors, Interleukin-2 / Q000172:deficiency; D014916:Whole-Body Irradiation
chemical_list: D018952:Antigens, CD34; D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse; D015375:Receptors, Interleukin-2