title: WTIP interacts with ASXL2 and blocks ASXL2-mediated activation of retinoic acid signaling.
abstract: The Asx-like (ASXL) family proteins are chromatin factors that play dual roles in transcriptional activation and repression. ASXL2 is highly expressed in the heart and is required for proper heart development and function. Here, we identify a novel ASXL2-binding partner, the LIM domain-containing protein WTIP. Genetic and biochemical assays show a direct interaction between ASXL2 and WTIP. In HeLa cells, ASXL2 enhances retinoic acid-dependent luciferase activity, while WTIP represses it. Furthermore, WTIP blocks ASXL2's stimulatory effect on transcription. In addition, we found that ASXL2 and WTIP are expressed in mouse embryonic epicardial cells, a tissue that is regulated by retinoic acid signaling. Together, these results implicate ASXL2 and WTIP in regulation of retinoic acid signaling during heart development.
authors: Department of Biological Sciences, University of Illinois at Chicago, 900 S. Ashland Ave., Chicago, IL 60607, USA.
authors: Farida F
authors: FF
authors: Khan
authors: Department of Biological Sciences, University of Illinois at Chicago, 900 S. Ashland Ave., Chicago, IL 60607, USA.
authors: Yanyang
authors: Y
authors: Li
authors: Department of Biological Sciences, University of Illinois at Chicago, 900 S. Ashland Ave., Chicago, IL 60607, USA.
authors: Arjun
authors: A
authors: Balyan
authors: Department of Biological Sciences, University of Illinois at Chicago, 900 S. Ashland Ave., Chicago, IL 60607, USA. Electronic address: qtwang@uic.edu.
authors: Q Tian
authors: QT
authors: Wang
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D056970:Co-Repressor Proteins; D003598:Cytoskeletal Proteins; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010496:Pericardium / Q000166:cytology / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements; D015398:Signal Transduction; D014212:Tretinoin / Q000378:metabolism* / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
chemical_list: C484295:ASXL2 protein, human; C484296:ASXL2 protein, mouse; D002352:Carrier Proteins; D056970:Co-Repressor Proteins; D003598:Cytoskeletal Proteins; D012097:Repressor Proteins; C484859:WTIP protein, human; C484858:Wtip protein, mouse; D014212:Tretinoin; D008156:Luciferases
keywords: ASXL2; Epicardium; Heart development; Retinoic acid signaling; WTIP