title: Generalized linear mixed model analysis of risk factors for contamination of moisture-enhanced pork with Campylobacter jejuni and Salmonella enterica Typhimurium.
abstract: Translocation of foodborne pathogens into the interior tissues of pork through moisture enhancement may be of concern if the meat is undercooked. In the present study, a five-strain mixture of Campylobacter jejuni or Salmonella enterica Typhimurium was evenly spread on the surface of fresh pork loins. Pork loins were injected, sliced, vacuum packaged, and stored. After storage, sliced pork was cooked by traditional grilling. Survival of Salmonella Typhimurium and C. jejuni in the interior tissues of the samples were analyzed by enumeration. The populations of these pathogens dropped below the detection limit (10 colony-forming units/g) in most samples that were cooked to 71.1°C or above. The general linear mixed model procedure was used to model the association between risk factors and the presence/absence of these pathogens after cooking. Estimated regression coefficients associated with the fixed effects indicated that the recovery probability of Salmonella Typhimurium was negatively associated with increasing level of enhancement. The effects of moisture enhancement and cooking on the recovery probability of C. jejuni were moderated by storage temperature. Our findings will assist food processors and regulatory agencies with science-based evaluation of the current processing, storage condition, and cooking guideline for moisture-enhanced pork.
authors: 1 Interdepartmental Microbiology Program, Iowa State University , Ames, Iowa.
authors: Xuesong
authors: X
authors: Wen
authors: Jing
authors: J
authors: Li
authors: James S
authors: JS
authors: Dickson
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D003296:Cooking; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D061353:Food Storage; D016014:Linear Models; D008460:Meat / Q000382:microbiology*; D012307:Risk Factors; D012486:Salmonella typhimurium / Q000302:isolation & purification*; D013552:Swine; D013696:Temperature