title: Evaluation of marine sediments as microbial sources for methane production from brown algae under high salinity.
abstract: Various marine sediments were evaluated as promising microbial sources for methane fermentation of Saccharina japonica, a brown alga, at seawater salinity. All marine sediments tested produced mainly acetate among volatile fatty acids. One marine sediment completely converted the produced volatile fatty acids to methane in a short period. Archaeal community analysis revealed that acetoclastic methanogens belonging to the Methanosarcina genus dominated after cultivation. Measurement of the specific conversion rate at each step of methane production under saline conditions demonstrated that the marine sediments had higher conversion rates of butyrate and acetate than mesophilic methanogenic granules. These results clearly show that marine sediments can be used as microbial sources for methane production from algae under high-salt conditions without dilution.
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan.
authors: Toyokazu
authors: T
authors: Miura
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan.
authors: Akihisa
authors: A
authors: Kita
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan.
authors: Yoshiko
authors: Y
authors: Okamura
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan.
authors: Tsunehiro
authors: T
authors: Aki
authors: Division of Energy and Environmental Engineering, Institute of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan; CREST, JST, Japan.
authors: Yukihiko
authors: Y
authors: Matsumura
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan.
authors: Takahisa
authors: T
authors: Tajima
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
authors: Junichi
authors: J
authors: Kato
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan; CREST, JST, Japan. Electronic address: nyutaka@hiroshima-u.ac.jp.
authors: Yutaka
authors: Y
authors: Nakashimada
mesh_terms: D001419:Bacteria / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000096:biosynthesis; D005285:Fermentation; D019015:Geologic Sediments / Q000382:microbiology*; D008697:Methane / Q000096:biosynthesis*; D000459:Phaeophyceae / Q000378:metabolism*; D054712:Salinity; D012623:Seawater / Q000382:microbiology*
chemical_list: D005232:Fatty Acids, Volatile; D008697:Methane
keywords: Brown algae; Marine macroalgae; Marine sediments; Methane production; Saccharina japonica