title: A novel label-free electrochemiluminescence aptasensor based on layered flowerlike molybdenum sulfide-graphene nanocomposites as matrix.
abstract: A label-free and ultrasensitive electrochemiluminescence (ECL) aptasensor was constructed for the detection of thrombin. Molybdenum sulfide-graphene nanocomposites with good conductivity and large surface area were immobilized on glassy carbon electrode (GCE), and then Nafion was fixed to chemosorb the Ru(bpy)3(2+) used as luminescence agent. Subsequently, gold nanoparticles (AuNPs) were modified on the electrode to immobilize the thiol-modified thrombin aptamer for fabrication of the thrombin aptasensor. The proposed ECL aptasensor produced the ultrasensitive detection of thrombin with a low detection limit of 3.6×10(-15)M (S/N=3) and over a wide target concentration range from 1.0×10(-14) to 5.0×10(-9)M. The aptasensor has been successfully applied in the determination of thrombin in human plasma samples of both traumatic and non-traumatic injury patients, indicating its promise in biochemical analysis. The recoveries of thrombin in human plasma samples are between 88.6% and 105.0%, and the RSD values are no more than 3.7%. The results demonstrate that this aptasensor has excellent sensitivity, selectivity and stability.
authors: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. Electronic address: liuym9518@sina.com.
authors: Yan-Ming
authors: YM
authors: Liu
authors: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
authors: Gui-Fang
authors: GF
authors: Shi
authors: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
authors: Jing-Jing
authors: JJ
authors: Zhang
authors: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
authors: Min
authors: M
authors: Zhou
authors: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
authors: Jun-Tao
authors: JT
authors: Cao
authors: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
authors: Ke-Jing
authors: KJ
authors: Huang
authors: Xinyang Central Hospital, Xinyang 464000, China.
authors: Shu-Wei
authors: SW
authors: Ren
mesh_terms: D055664:Electrochemical Techniques; D006108:Graphite / Q000737:chemistry*; D049449:Luminescence; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008982:Molybdenum / Q000737:chemistry*; D053761:Nanocomposites; D013440:Sulfides / Q000737:chemistry*
chemical_list: D013440:Sulfides; D006108:Graphite; D008982:Molybdenum
keywords: Aptasensor; Electrochemiluminescence; Molybdenum sulfide–graphene nanocomposites; Thrombin