title: Different microRNA profiles in chronic epilepsy versus acute seizure mouse models.
abstract: Epilepsy affects around 50 million people worldwide, and in about 65% of patients, the etiology of disease is unknown. MicroRNAs are small non-coding RNAs that have been suggested to play a role in the pathophysiology of epilepsy. Here, we compared microRNA expression patterns in the hippocampus using two chronic models of epilepsy characterised by recurrent spontaneous seizures (pilocarpine and self-sustained status epilepticus (SSSE)) and an acute 6-Hz seizure model. The vast majority of microRNAs deregulated in the acute model exhibited increased expression with 146 microRNAs up-regulated within 6 h after a single seizure. In contrast, in the chronic models, the number of up-regulated microRNAs was similar to the number of down-regulated microRNAs. Three microRNAs-miR-142-5p, miR-331-3p and miR-30a-5p-were commonly deregulated in all three models. However, there is a clear overlap of differentially expressed microRNAs within the chronic models with 36 and 15 microRNAs co-regulated at 24 h and at 28 days following status epilepticus, respectively. Pathway analysis revealed that the altered microRNAs are associated with inflammation, innate immunity and cell cycle regulation. Taken together, the identified microRNAs and the pathways they modulate might represent candidates for novel molecular approaches for the treatment of patients with epilepsy.
authors: Institute of Pharmacology and Toxicology, University of Bonn, Sigmund-Freud-Str. 25, 53127, Bonn, Germany.
authors: Anita
authors: A
authors: Kretschmann
authors: Benedicte
authors: B
authors: Danis
authors: Lidija
authors: L
authors: Andonovic
authors: Khalid
authors: K
authors: Abnaof
authors: Marijke
authors: M
authors: van Rikxoort
authors: Franziska
authors: F
authors: Siegel
authors: Manuela
authors: M
authors: Mazzuferi
authors: Patrice
authors: P
authors: Godard
authors: Etienne
authors: E
authors: Hanon
authors: Holger
authors: H
authors: Fröhlich
authors: Rafal M
authors: RM
authors: Kaminski
authors: Patrik
authors: P
authors: Foerch
authors: Alexander
authors: A
authors: Pfeifer
mesh_terms: D000818:Animals; D018816:Genes, cdc; D006624:Hippocampus / Q000378:metabolism; D007113:Immunity, Innate / Q000235:genetics; D007249:Inflammation / Q000235:genetics; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012640:Seizures / Q000235:genetics* / Q000378:metabolism; D013226:Status Epilepticus / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs