title: Optimization of storage condition for maintaining long-term viability of nematophagous fungus Esteya vermicola as biocontrol agent against pinewood nematode.
abstract: The fungus, Esteya vermicola has been proposed as biocontrol agent against pine wilting disease caused by Bursaphelenchus xylophilus. In this study, we reported the effects of temperature and different additives on the viability and biocontrol efficacy of E. vermicola formulated by alginate-clay. The viability of the E. vermicola formulation was determined for six consecutive months at temperature ranged from -70 to 25 °C. The fresh conidia without any treatment were used as control. Under the optimal storage conditions with E. vermicola alginate-clay formulation, the results suggested that E. vermicola alginate-clay formulation with a long shelf life could be a non-vacuum-packed formulation that contains 2 % sodium alginate and 5 % clay at 4 °C. Three conidial formulations prepared with additives of 15 % glycerol, 0.5 % yeast extract and 0.5 % herbal extraction, respectively significantly improved the shelf life. In addition, these tested formulations retained the same biocontrol efficacy as the fresh conidial against pinewood nematode. This study provided a tractable and low-cost method to preserve the shelf life of E. vermicola.
authors: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Taejon, 305-764, Republic of Korea, xuejianjie@163.com.
authors: Jian Jie
authors: JJ
authors: Xue
authors: Jin Gang
authors: JG
authors: Hou
authors: Yong An
authors: YA
authors: Zhang
authors: Chun Yan
authors: CY
authors: Wang
authors: Zhen
authors: Z
authors: Wang
authors: Jiao Jiao
authors: JJ
authors: Yu
authors: Yun Bo
authors: YB
authors: Wang
authors: Yu Zhu
authors: YZ
authors: Wang
authors: Qing Hua
authors: QH
authors: Wang
authors: Chang Keun
authors: CK
authors: Sung
mesh_terms: D000464:Alginates / Q000378:metabolism; D000538:Aluminum Silicates / Q000378:metabolism; D000818:Animals; D000077215:Clay; D020723:Glucuronic Acid / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D050296:Microbial Viability; D009348:Nematoda / Q000382:microbiology / Q000502:physiology; D055155:Ophiostomatales / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D010461:Peptones / Q000378:metabolism; D010572:Pest Control, Biological / Q000379:methods; D010936:Plant Extracts / Q000378:metabolism; D011309:Preservation, Biological / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D000464:Alginates; D000538:Aluminum Silicates; D006603:Hexuronic Acids; D010461:Peptones; D010936:Plant Extracts; D020723:Glucuronic Acid; D005990:Glycerol; D000077215:Clay