title: Investigation of total phenolic content and antioxidant activities of Azadirachta indica roots.
abstract: OBJECTIVE
  The present study was an attempt to study total phenolic content and antioxidant property of the crude ethanolic extract of the roots of Azadirachta indica (A. indica).
  
  
  METHODS
  To evaluate the antioxidant properties of the crude extract, some complementary test systems, namely DPPH free radical scavenging assay, reducing power assay, and ferrous ion chelating ability and determination of total phenolic content were conducted.
  
  
  RESULTS
  In DPPH free radical scavenging test, IC50 value of the crude extract was found to be fairly significant (13.81±0.06 μg/ml) while compared with that of the reference standards, ascorbic acid and BHA (2.12±0.02 and 4.87±0.05 μg/ml, respectively). In reducing power assay, the maximum absorbance for the extract was found to be 1.523±0.026 at100 μg/ml compared with standard ascorbic acid and BHA (2.811±0.013 μg/ml and 2.031±0.019 μg/ml, respectively). The IC50 value of the extract as percentage of Fe(++) ion chelating ability was determined as 19.01±0.024 μg/ml where EDTA showed 8.87±0.035 μg/ml. The total phenolic amount was also calculated quite high in the extract (238.81±0.98 mg/g of gallic acid equivalent).
  
  
  CONCLUSIONS
  The assays showed the presence of significant antioxidant properties of the crude sample, which would justify its traditional use. However, it would be very interesting to investigate the possible causes and their mechanisms responsible for the antioxidant property of the plant A. indica.
authors: Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
authors: Md Delowar
authors: MD
authors: Hossain
authors: Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
authors: Md Shahid
authors: MS
authors: Sarwar
authors: Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
authors: Syed Masudur Rahman
authors: SM
authors: Dewan
authors: Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
authors: Md Shohel
authors: MS
authors: Hossain
authors: Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
authors: Afm
authors: A
authors: Shahid-Ud-Daula
authors: Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
authors: Mohammad Safiqul
authors: MS
authors: Islam
keywords: Activities DPPH Free Radical; Antioxidant; Azadirachta indica; Scavenging; Total Penolic Content