title: A study of the mechanical properties and cytocompatibility of lactide and caprolactone based scaffolds filled with inorganic bioactive particles.
abstract: The mechanical properties of highly porous (90% porosity) poly(l-lactide) (PLLA), poly(ε-caprolactone) (PCL) and poly(l-lactide/ε-caprolactone) (PLCL) were investigated. Young's modulus of non-porous PLLA, PCL and PLCL dropped from 2263.4, 183.7 and 5.7 MPa to 16.8, 1.0 and 1.0 MPa, respectively, for their ~90% porous counterparts. Elongation at break of PCL decreased noticeably with porosity fraction while PLCL maintained a highly elastomeric character and strain recovery capacity even in the presence of pores. Inorganic bioactive particles (hydroxyapatite or bioglass) were added to confer bioactivity to the aforementioned synthetic bioresorbable polymers, and their effect on the mechanical properties was also investigated. Addition of 15 vol.% of inorganic bioactive particles increased the Young's modulus of highly porous PLLA from 16.2 to ~30 MPa. On the contrary, the difference between Young's modulus of filled and unfilled PCL and PLCL scaffolds was not statistically significant. Finally, an in vitro study of the cytocompatibility and adhesion of adipose derived stem cells (ADSCs) was conducted. The observed viability and excellent adhesion of these cells to both porous and non-porous templates indicate that the employed materials can be good candidates for application in tissue engineering.
authors: University of the Basque Country (UPV/EHU), Department of Mining-Metallurgy Engineering and Materials Science & POLYMAT, School of Engineering, Alameda de Urquijo s/n, 480130 Bilbao, Spain.
authors: A
authors: A
authors: Larrañaga
authors: University of the Basque Country (UPV/EHU), Department of Mining-Metallurgy Engineering and Materials Science & POLYMAT, School of Engineering, Alameda de Urquijo s/n, 480130 Bilbao, Spain.
authors: E
authors: E
authors: Diamanti
authors: University of the Basque Country (UPV/EHU), Faculty of Medicine and Odontology, Bilbao, Spain.
authors: E
authors: E
authors: Rubio
authors: University of the Basque Country (UPV/EHU), Faculty of Medicine and Odontology, Bilbao, Spain.
authors: T
authors: T
authors: Palomares
authors: University of the Basque Country (UPV/EHU), Faculty of Medicine and Odontology, Bilbao, Spain.
authors: A
authors: A
authors: Alonso-Varona
authors: Donostia University Hospital (Osakidetza-Basque Health Service) & BIODONOSTIA, San Sebastián, Spain.
authors: P
authors: P
authors: Aldazabal
authors: Donostia University Hospital (Osakidetza-Basque Health Service) & BIODONOSTIA, San Sebastián, Spain.
authors: F J
authors: FJ
authors: Martin
authors: University of the Basque Country (UPV/EHU), Department of Mining-Metallurgy Engineering and Materials Science & POLYMAT, School of Engineering, Alameda de Urquijo s/n, 480130 Bilbao, Spain. Electronic address: jr.sarasua@ehu.es.
authors: J R
authors: JR
authors: Sarasua
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D002208:Caproates / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004146:Dioxanes / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D017886:Durapatite; D055119:Elastic Modulus; D006801:Humans; D007783:Lactones / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D008422:Materials Testing; D016062:Porosity; D013234:Stem Cells; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002208:Caproates; D004146:Dioxanes; D007783:Lactones; C121056:caprolactone; D017886:Durapatite; C091880:dilactide
keywords: Adipose derived stem cells; Bioglass; Hydroxyapatite; Polylactides; Polylactones