title: A lysine deacetylase Hos3 is targeted to the bud neck and involved in the spindle position checkpoint.
abstract: An increasing number of cellular activities can be regulated by reversible lysine acetylation. Targeting the enzymes responsible for such posttranslational modifications is instrumental in defining their substrates and functions in vivo. Here we show that a Saccharomyces cerevisiae lysine deacetylase, Hos3, is asymmetrically targeted to the daughter side of the bud neck and to the daughter spindle pole body (SPB). The morphogenesis checkpoint member Hsl7 recruits Hos3 to the neck region. Cells with a defect in spindle orientation trigger Hos3 to load onto both SPBs. When associated symmetrically with both SPBs, Hos3 functions as a spindle position checkpoint (SPOC) component to inhibit mitotic exit. Neck localization of Hos3 is essential for its symmetric association with SPBs in cells with misaligned spindles. Our data suggest that Hos3 facilitates cross-talk between the morphogenesis checkpoint and the SPOC as a component of the intricate monitoring of spindle orientation after mitotic entry and before commitment to mitotic exit.
authors: Program in Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853 Department of Molecular Medicine, Cornell University, Ithaca, NY 14853.
authors: Mengqiao
authors: M
authors: Wang
authors: Department of Molecular Medicine, Cornell University, Ithaca, NY 14853 ruth.collins@cornell.edu.
authors: Ruth N
authors: RN
authors: Collins
mesh_terms: D000107:Acetylation; D006655:Histone Deacetylases / Q000502:physiology*; D059566:M Phase Cell Cycle Checkpoints; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D011484:Protein-Arginine N-Methyltransferases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism / Q000502:physiology*; D058112:Septins / Q000378:metabolism; D008941:Spindle Apparatus / Q000201:enzymology*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D011484:Protein-Arginine N-Methyltransferases; C120666:HSL7 protein, S cerevisiae; D006655:Histone Deacetylases; C000598222:Hos3 protein, S cerevisiae; D058112:Septins