title: Treatment with lutein provides neuroprotection in mice subjected to transient cerebral ischemia.
abstract: Lutein is known to be a nonprovitamin A carotenoid found in broccoli and spinach. The aim of present study was to investigate whether lutein can protect brain against ischemic injury by reducing oxidative stress. Male ICR mice were randomly divided into five experimental groups: model group, sham group, lutein high, middle, and low-dose groups (30, 15, and 7.5 mg/kg). Mice were subjected to a 2-h middle cerebral artery occlusion followed by reperfusion for 22 h. The reduced glutathione/oxidized glutathione (GSH/GSSG) ratio, antioxidant enzyme activities, malondialdehyde (MDA), and the carbonyl content in oxidatively modified proteins in brain tissue were determined with colorimetric method. The 8-hydroxy deoxyguanosine (8-OHdG) expression was measured by immunohistochemistry assay, and the neuron apoptosis was detected by TdT-mediated dUTP nick end labeling assay. Then, the neurological deficit scores were measured at last. Treatment of lutein significantly elevated the ratio of GSH/GSSG as well as activities of superoxide dismutase, glutathione peroxidase, and catalase and obviously decreased the contents of MDA, brain carbonyl, the expression of 8-OHdG, the number of apoptotic cells, and neurological deficit scores. Our results demonstrate that administration of lutein affords strong neuroprotective effect against transient cerebral ischemic injury and that the effect might be associated with its antioxidant property.
authors: a Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University , Beijing 100191 , China.
authors: Ya-Xuan
authors: YX
authors: Sun
authors: Ting
authors: T
authors: Liu
authors: Xue-Ling
authors: XL
authors: Dai
authors: Qiu-Sheng
authors: QS
authors: Zheng
authors: Bo-Di
authors: BD
authors: Hui
authors: Zhao-Feng
authors: ZF
authors: Jiang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000188:drug therapy*; D014975:Lutein / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D014975:Lutein
keywords: antioxidant; lutein; neuroprotection; oxidative stress; transient cerebral ischemia