title: Effects of sodium hypochlorite on Agave tequilana Weber bagasse fibers used to elaborate cyto and biocompatible hydrogel films.
abstract: Waste bagasse of Agave tequilana-Weber fibers treated with sodium hydroxide was used to elaborate hydrogel films. The bagasse was offered in an alternative use for the preparation of hydrogel films by phase inversion method without crosslinking and further purification of cellulose. The effect on the properties of the obtained films was studied when the chemical treatment of the agave fibers was changed. It was found that the resultant hydrogels showed increment in tensile from 40 N/mm(2) to 56 N/mm(2) with the increase of sodium hypochlorite concentration from 1 to 10 vol.%, respectively. With regard to biocompatibility properties of the hydrogel films, platelet adhesion, clotting time and protein adsorption were investigated. Analysis of the morphology of adherent NIH3T3 fibroblast indicated that the projected cell area, aspect ratio and long axis gradually increased with the increment of sodium hypochlorite content in the agave treatment. It was presented that the chemical treatment affects cell adhesion and morphology and lignin content remains in the brown fibers.
authors: Department of Materials Science and Technology, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan; Biomedical Science Institute, Universidad Autonoma de Cd. Juárez, Estocolmo y anillo envolvente del PRONAF CP 32315, Mexico.
authors: Karla Lizette
authors: KL
authors: Tovar-Carrillo
authors: Department of Materials Science and Technology, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
authors: Kazuki
authors: K
authors: Nakasone
authors: Department of Chemistry, Universidad de Guanajuato, Lascurain de Retana 5, Centro, 36000 Guanajuato, Mexico.
authors: Satoshi
authors: S
authors: Sugita
authors: Department of Materials Science and Technology, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
authors: Motohiro
authors: M
authors: Tagaya
authors: Department of Materials Science and Technology, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan. Electronic address: takaomi@nagaokaut.ac.jp.
authors: Takaomi
authors: T
authors: Kobayashi
mesh_terms: D027882:Agave / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D001792:Blood Platelets / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry*; D008756:Methylgalactosides / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D041681:NIH 3T3 Cells; D010973:Platelet Adhesiveness; D012973:Sodium Hypochlorite / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D008756:Methylgalactosides; C109801:hydrogel film; D002482:Cellulose; C027433:bagasse; D012973:Sodium Hypochlorite
keywords: Agave; Biocompatibility; Cytocompatibility; Hydrogel; Treatment