title: Determination of six polyynes in Oplopanax horridus and Oplopanax elatus using polyethylene glycol modified reversed migration microemulsion electrokinetic chromatography.
abstract: A PEG-modified reversed migration MEEKC method was developed for simultaneous determination of six polyynes, including oplopandiol, falcarindiol, oplopandiol acetate, (11S, 16S, 9Z)-9,17-octadecadiene-12,14-diyne-1,11,16-triol,1-acetate, oplopantriol B, and oplopantriol A, in Oplopanax horridus and Oplopanax elatus. The running buffer containing 0.8% v/v ethyl acetate, 3.8% w/v SDS, 6.6% v/v n-butanol in 20 mM phosphate buffer (pH 2.5), followed by mixing with propan-2-ol at 30% v/v and PEG-1000 at 15% w/v, was applied in the analysis. The proposed method was successfully applied to determine the six polyynes in five samples of Oplopanax horridus and one of O. elatus. The result showed that the types and amounts of polyynes present were obviously different when comparing the two herbs. Besides, the developed PEG-modified reversed MEEKC method might be suitable for the analysis of hydrophobic analytes in herbal medicines.
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
authors: Zhong-mei
authors: ZM
authors: Yang
authors: Jing
authors: J
authors: Zhao
authors: Ka-meng
authors: KM
authors: Lao
authors: Xiao-jia
authors: XJ
authors: Chen
authors: Fong
authors: F
authors: Leong
authors: Chong-Zhi
authors: CZ
authors: Wang
authors: Chun-Su
authors: CS
authors: Yuan
authors: Shao-ping
authors: SP
authors: Li
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D028002:Oplopanax / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D053279:Polyynes / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D053279:Polyynes; D011092:Polyethylene Glycols
keywords: Oplopanax; PEG; Polyynes; Reversed migration MEEKC