title: The Sequence Saturation Mutagenesis (SeSaM) method.
abstract: Sequence Saturation Mutagenesis (SeSaM) is a random mutagenesis method developed to overcome the limitations of existing error-prone PCR (epPCR) protocols. SeSaM is advantageous with respect to (1) elimination of mutagenic "hot spots", (2) increase in frequency of subsequent nucleotide substitutions, (3) control over the mutational bias through the utilization of universal base analogs, and, consequently, (4) the prospect of generating transversion-enriched mutant libraries. These advanced features lead to chemically diverse mutant libraries on the protein level, essentially making SeSaM a complementary technology to transition biased epPCR mutagenesis methods.
authors: Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, Aachen, 52074, Germany.
authors: Anna Joëlle
authors: AJ
authors: Ruff
authors: Tsvetan
authors: T
authors: Kardashliev
authors: Alexander
authors: A
authors: Dennig
authors: Ulrich
authors: U
authors: Schwaneberg
mesh_terms: D019020:Directed Molecular Evolution / Q000379:methods; D016296:Mutagenesis / Q000235:genetics*; D016133:Polymerase Chain Reaction