title: Oleanolic acid regulates NF-κB signaling by suppressing MafK expression in RAW 264.7 cells.
abstract: Oxidative stress and inflammation are common to many pathological conditions. Defense mechanisms protect cells from oxidative stress, but can become over-activated following injury and inflammation. NF-κB and Nrf2 transcription factors regulate proinflammatory and antioxidant gene expression, respectively. Studies have shown that many natural dietary compounds regulate NF-κB and Nrf2, preventing inflammation and oxidative stress. Here, we report major compounds of Prunella vulgaris var. lilacina such as rosmarinic acid, oleanolic acid, ursolic acid and caffeic acid as a potential therapeutic for oxidative stress and inflammation. The major compounds exhibited high anti-inflammatory activity, inhibiting NO, PGE2 production, NF-κB expression and activating Nrf2 expression. In addition, we examined the effect of major compounds on MafK expression. Among the compounds, oleanolic acid significantly decreased MafK expression and MafK-mediated p65 acetylation. These findings suggest that oleanolic acid as NF-κB inhibitors can potentially be used in therapeutic applications for the treatment of oxidative stressnduced diseases.
authors: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Wanju 565-850, Korea.
authors: Yu-Jin
authors: YJ
authors: Hwang
authors: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors: Jaewhan
authors: J
authors: Song
authors: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Wanju 565-850, Korea.
authors: Haeng-Ran
authors: HR
authors: Kim
authors: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Wanju 565-850, Korea.
authors: Kyung-A
authors: KA
authors: Hwang
mesh_terms: D000107:Acetylation; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D015232:Dinoprostone / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D050985:MafK Transcription Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D009828:Oleanolic Acid / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D031347:Prunella / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D050985:MafK Transcription Factor; C494696:Mafk protein, mouse; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; D034741:RNA, Small Interfering; D051996:Transcription Factor RelA; D009569:Nitric Oxide; D009828:Oleanolic Acid; D015232:Dinoprostone