title: Neuraminidase Inhibitors from the Fermentation Broth of Phellinus linteus.
abstract: During a search for neuraminidase inhibitors derived from medicinal fungi, we found that the fermentation broth of Phellinus linteus exhibited potent neuraminidase inhibitory activity. Through bioassay-guided fractionation, two active compounds were purified from the ethyl acetate-soluble portion of the fermentation broth of P. linteus. These structures were identified as inotilone (1) and 4-(3,4-dihydroxyphenyl)-3-buten-2-one (2) by spectroscopic methods. Compounds 1 and 2 inhibited H1N1 neuraminidase activity with IC50 values of 29.1 and 125.6 µM, respectively, in a dose-dependent manner. They also exhibited an antiviral effect in a viral cytopathic effect reduction assay using MDCK cells. These results suggest that compounds 1 and 2 from the culture broth of P. linteus would be good candidates for the prevention and therapeutic strategies towards viral infections.
authors: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors: Byung Soon
authors: BS
authors: Hwang
authors: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors: Myeong-Seok
authors: MS
authors: Lee
authors: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors: Seung Woong
authors: SW
authors: Lee
authors: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors: In-Kyoung
authors: IK
authors: Lee
authors: Korea National College of Agriculture and Fisheries, Hwaseong 445-760, Korea.
authors: Geon-Sik
authors: GS
authors: Seo
authors: Department of Beauty Science, Kwangju Women's University, Gwangju 506-713, Korea.
authors: Hwa Jung
authors: HJ
authors: Choi
authors: Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea.
authors: Bong-Sik
authors: BS
authors: Yun
keywords: 4-(3,4-Dihydroxyphenyl)-3-buten-2-one; Anti-influenza agent; Inotilone; Neuraminidase inhibitor; Phellinus linteus