title: Fluorometric determination of free glucose and glucose 6-phosphate in cows' milk and other opaque matrices.
abstract: Analyses of free glucose and glucose 6-phosphate in milk have until now been dependent upon several time consuming and troublesome procedures. This has limited investigations in the area. The present article presents a new, reliable, analytical procedure, based on enzymatic degradation and fluorometric detection. Standards and control materials were based on milk that was stripped of intrinsic glucose and glucose 6-phosphate in order to obtain standards and samples based on the same matrix. The analysis works without pre-treatment of the samples, e.g. without centrifugation and precipitation of protein with acids.
authors: Dept. of Animal Science, Aarhus University, Blichers Allé 20, Postboks 50, DK-8830 Tjele, Denmark. Electronic address: Torben.Larsen@agrsci.dk.
authors: Torben
authors: T
authors: Larsen
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D005470:Fluorometry / Q000379:methods*; D005947:Glucose / Q000737:chemistry*; D019298:Glucose-6-Phosphate / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*
chemical_list: D019298:Glucose-6-Phosphate; D005947:Glucose
keywords: Free glucose; Glucose 6-phosphate; Milk matrix; Monosaccharides