title: Nanocomplex based on biocompatible phospholipids and albumin for long-circulation applications.
abstract: Achieving long circulating delivery of nanoparticles (NPs) is important for efficient drug therapy, but it is difficult due largely to proteins adsorption (opsonization) or/and nonsufficient stability of NPs. In this present work, we aimed to address the above issues by constructing a phospholipid and BSA-based nanocomplex system, namely BSA-phospholipid NPs (BSA-PL-NPs). Combining sodium dodecyl sulfate-polyacrylamide gel electrophoresis, X-ray photoelectron spectroscopy and proteins adsorption property, we confirmed that some BSA molecules were fixed on the inner surface of BSA-PL-NPs via hydrophobic interactions and the others were located in the core area. This special configuration allowed BSA-PL-NPs to not only maintain the antiadsorption and low phagocytosis properties but also have the slow zero-order drug release and the enhanced nanostructure stability. Interestingly, we found that BSA-PL-NPs had no cytotoxicity to mouse L929 fibroblasts but could stimulate the cells' growth instead. In conclusion, BSA-PL-NPs have a great potential to be developed as a long-circulation drug delivery system, and the ready availability, biocompatibility and nontoxicity of phospholipids and albumin give this system great promise for practical use.
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu 610041, China.
authors: Qiang
authors: Q
authors: Peng
authors: Xue-Qin
authors: XQ
authors: Wei
authors: Xiao-Ru
authors: XR
authors: Shao
authors: Ting
authors: T
authors: Zhang
authors: Shu
authors: S
authors: Zhang
authors: Na
authors: N
authors: Fu
authors: Xiao-Xiao
authors: XX
authors: Cai
authors: Zhi-Rong
authors: ZR
authors: Zhang
authors: Yun-Feng
authors: YF
authors: Lin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D002460:Cell Line; D003374:Coumarins / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010316:Particle Size; D010587:Phagocytosis; D010743:Phospholipids / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D055672:Static Electricity; D013844:Thiazoles / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D003374:Coumarins; D010743:Phospholipids; D013844:Thiazoles; C517282:coumarin 6; D012710:Serum Albumin, Bovine