title: Oxidative stress of alternariol in Caco-2 cells.
abstract: Alternariol (AOH) is a mycotoxin produced by fungus Alternaria. It is found in a wide variety of fruits and cereals products. AOH is able to damage human health. The aim of this study was to evaluate the cytotoxicity of AOH in human colon adenocarcinoma (Caco-2) cells. Moreover, some events related to oxidative stress were evaluated: reactive oxygen species (ROS) generated by oxidation of 2',7'-dichlorodihydrofluorescein diacetate; peroxidation of lipid (LPO) by malondialdehyde (MDA) production; and antioxidant enzymatic capability of catalase (CAT) and superoxide dismutase (SOD). Cytotoxicity of AOH (from 3.125 to 100 μM) was determined during 24, 48 and 72 h of exposure by different endpoints. AOH decreased cell viability by MTT, NR and PC assays. However, no IC50 values were obtained by any of the assays tested. AOH induced a strong oxidative stress in Caco-2 cells by generation of ROS production and LPO associated with a rise in the SOD activity at all concentration tested. ROS increased 1.2-fold with respect to the control and MDA production ranged from 130% to 250% compared to control. Our results demonstrated that in spite of AOH showing cytotoxic effect on Caco-2 cells at the highest concentration tested, oxidative stress by LPO and ROS was observed at all concentrations assayed. This could cause an injury and be hazardous to health.
authors: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain. Electronic address: celia.fernandez-blanco@uv.es.
authors: Celia
authors: C
authors: Fernández-Blanco
authors: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
authors: Guillermina
authors: G
authors: Font
authors: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
authors: Maria-Jose
authors: MJ
authors: Ruiz
mesh_terms: D018938:Caco-2 Cells / Q000187:drug effects; D002374:Catalase / Q000187:drug effects; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007783:Lactones / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000032:analysis; D009183:Mycotoxins / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000032:analysis; D013482:Superoxide Dismutase / Q000187:drug effects
chemical_list: D007783:Lactones; D009183:Mycotoxins; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; C005197:alternariol
keywords: Alternariol; Antioxidant defense; Caco-2 cells; Oxidative stress