title: Gene expression profiles associated with osteoblasts differentiated from bone marrow stromal cells.
abstract: OBJECTIVE
  To study the changes of gene expression profiles associated with osteoblasts differentiated from rat bone marrow stromal cells in vitro by gene chip technique.
  
  
  METHODS
  rat Bone marrow stromal cells were isolated and cultured, and differentiation was induced by dexamethasone, β-glycerol phosphate and vitamin C. Cellular mRNA was extracted and reverse transcribed into cDNA, thus related genes expression differences were detected by gene expression profile chip.
  
  
  RESULTS
  Calcifying nodules were visible in the induced cells. There were 27.7% genes expressed differentially, three times more than the normal and induced cells, and some genes were related to transcription, translation, glycosylation modification. Extracellular matrix, signal molecules and metabolism were up-regulated.
  
  
  CONCLUSIONS
  The gene chip technique can be used to detect the multi-gene different expression in the differentiation-induced rat BMSCs, and these differentially expressed genes are necessary genes related to rat BMSCs proliferation and induction of osteoblastic differentiation.
authors: Thrombosis Department, Shandong Traffic Hospital, Jinan 250031, Shandong, China.
authors: Lu
authors: L
authors: Lu
authors: Laboratory Department, Shandong Traffic Hospital, Jinan 250031, Shandong, China.
authors: Yang
authors: Y
authors: Gao
authors: Laboratory Department, Shandong provincial Qianfoshan Hospital Affiliated to Shandong University, Jinan 250031, Shandong, China. Electronic address: miauxu@163.com.
authors: Miao
authors: M
authors: Xu
authors: Regenerative Medicine Research Laboratory, Shandong Traffic Hospital, Jinan 250031, Shandong, China.
authors: Ru-Cun
authors: RC
authors: Ge
authors: Students' Affairs Department, Shandong University of Traditional Chinese Medicine, Jinan 250031, Shandong, China.
authors: Lin
authors: L
authors: Lu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D059467:Transcriptome / Q000235:genetics / Q000502:physiology*
keywords: Bone marrow stromal cells; Differentiation-inducing; Gene chip; Gene expression profile; Osteoblasts