title: Gene expression profiles of patients with cerebral hematoma following spontaneous intracerebral hemorrhage.
abstract: The present study aimed to investigate the gene functions and expression profiles in perihematomal (PH) brain regions following spontaneous intracerebral hemorrhage. The gene expression profiles were downloaded from the Gene Expression Omnibus database under accession number GSE24265, which includes 11 brain samples from different regions, including four samples from PH areas, four from contralateral grey matter (CG) and three from contralateral white matter (CW). The gene expression profiles were pre-processed and the differentially expressed genes (DEGs) between PH and CG tissue, and PH and CW tissue were identified using R packages. The expression of genes in different tissues was analyzed by hierarchical clustering. Then, the interaction network between the DEGs was constructed using String software. Finally, Gene Ontology was performed and pathway analysis was conducted using FuncAssociate and Expression Analysis Systematic Explorer to identify the gene function. As a result, 399 DEGs were obtained between PH and CG, and 756 DEGs were identified between PH and CW. There were 35 common DEGs between the two groups. These DEGs may be involved in PH edema by regulating the calcium signaling pathway [calcium channel, voltage‑dependent, T-type, α1I subunit, Ca2+/calmodulin‑dependent protein kinase II α (CAMK2A), ryanodine receptor 2 (RYR2) and inositol 1,4,5-trisphosphate receptor, type 1 (ITPR1)], cell proliferation (sphingosine kinase 1), neuron differentiation (Ephrin-A5) or extracellular matrix-receptor interaction [collagen, type I, α 2, laminin B1 (LAMB1), syndecan 2, fibronectin 1 and integrin α5 (ITGA5)]. A number of genes may cooperate to participate in the same pathway, such as ITPR1-RYR2, CAMK2A-RYR2 and ITGA5-LAMB1 interaction pairs. The present study provides several potential targets to decrease hematoma expansion and alleviate neuronal cell death following spontaneous intracerebral hemorrhage.
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Tao
authors: T
authors: Yang
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Jianwen
authors: J
authors: Gu
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Bin
authors: B
authors: Kong
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Yongqin
authors: Y
authors: Kuang
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Lin
authors: L
authors: Cheng
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Jingmin
authors: J
authors: Cheng
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Xun
authors: X
authors: Xia
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Yuan
authors: Y
authors: Ma
authors: Department of Neurosurgery, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China.
authors: Junhai
authors: J
authors: Zhang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D020013:Calcium Signaling / Q000235:genetics; D002543:Cerebral Hemorrhage / Q000378:metabolism / Q000473:pathology*; D016000:Cluster Analysis; D036386:Ephrin-A5 / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006801:Humans; D008297:Male; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D012984:Software; D000099157:Sphingosine Kinase
chemical_list: D036386:Ephrin-A5; D017853:Phosphotransferases (Alcohol Group Acceptor); D000099157:Sphingosine Kinase