title: Shrink-induced biomimetic wrinkled substrates for functional cardiac cell alignment and culture.
abstract: The anisotropic alignment of cardiomyocytes in native myocardium tissue is a functional feature that is absent in traditional in vitro cardiac cell culture. Microenvironmental factors cue structural organization of the myocardium, which promotes the mechanical contractile properties and electrophysiological patterns seen in mature cardiomyocytes. Current nano- and microfabrication techniques, such as photolithography, generate simplified cell culture topographies that are not truly representative of the multifaceted and multi-scale fibrils of the cardiac extracellular matrix. In addition, such technologies are costly and require a clean room for fabrication. This chapter offers an easy, fast, robust, and inexpensive fabrication of biomimetic multi-scale wrinkled surfaces through the process of plasma treating and shrinking prestressed thermoplastic. Additionally, this chapter includes techniques for culturing stem cells and their cardiac derivatives on these substrates. Importantly, this wrinkled cell culture platform is compatible with both fluorescence and bright-field imaging; real-time physiological monitoring of CM action potential propagation and contraction properties can elucidate cardiotoxicity drug effects.
authors: Department of Biomedical Engineering, University of California, 5200 Engineering Hall Irvine, Irvine, CA, USA.
authors: Nicole
authors: N
authors: Mendoza
authors: Roger
authors: R
authors: Tu
authors: Aaron
authors: A
authors: Chen
authors: Eugene
authors: E
authors: Lee
authors: Michelle
authors: M
authors: Khine
mesh_terms: D016880:Anisotropy; D001696:Biomechanical Phenomena; D040761:Biomimetic Materials / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D055595:Mechanical Phenomena; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects*; D020959:Polyethylene / Q000494:pharmacology*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D020959:Polyethylene