title: Establishment of ATP-based luciferase viability assay in 96-well plate for Trypanosoma congolense.
abstract: Animal African trypanosomosis (AAT), caused by Trypanosoma congolense, is widespread throughout sub-Saharan Africa. There are significant concerns related to the current drugs available for the treatment of AAT due to their limited effectiveness across species and their adverse effects. Moreover, drug resistant trypanosomes have recently been reported in the field. High throughput screening (HTS) of large chemical compound library collections is a promising approach for identifying novel drug candidates. While HTS for Trypanozoon trypanosomes, T. brucei sspp. and T. evansi is well established, no assays have been developed for T. congolense. In the present study, the authors developed an ATP-based luciferase viability assay for T. congolense in a 96-well plate format. The calculated 50% inhibitory concentration (IC50) values for pentamidine and diminazene were 10-100 times higher in T. congolense than in T. brucei. This result suggests that the transporters for the 2 tested compounds differ between T. congolense and T. brucei. This assay could further be applied to screen novel chemical compounds for the treatment of AAT caused by T. congolense.
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors: Keisuke
authors: K
authors: Suganuma
authors: Puttik
authors: P
authors: Allamanda
authors: Hassan
authors: H
authors: Hakimi
authors: Mo
authors: M
authors: Zhou
authors: Jose Ma
authors: JM
authors: Angeles
authors: Shin-ichiro
authors: S
authors: Kawazu
authors: Noboru
authors: N
authors: Inoue
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000502:physiology; D004121:Dimethyl Sulfoxide; D004133:Diminazene / Q000494:pharmacology*; D057166:High-Throughput Screening Assays / Q000379:methods / Q000662:veterinary*; D020128:Inhibitory Concentration 50; D008156:Luciferases; D010419:Pentamidine / Q000494:pharmacology*; D013045:Species Specificity; D013997:Time Factors; D014348:Trypanosoma congolense / Q000187:drug effects*; D014353:Trypanosomiasis, African / Q000188:drug therapy* / Q000662:veterinary*
chemical_list: D010419:Pentamidine; D000255:Adenosine Triphosphate; D008156:Luciferases; D004133:Diminazene; D004121:Dimethyl Sulfoxide