title: [Effect of CCL3 on osteoblast in myeloma bone disease].
abstract: OBJECTIVE
  To culture osteoblast in vitro and evaluate CCL3 receptor CCR1 expression in patients with multiple myeloma (MM).
  
  
  METHODS
  Bone marrow osteoblasts from MM patients were cultured in vitro with dexamethasone, β-sodium glycerophosphate and vitamin C, which were identified by alkaline phosphatase staining, Von Kossa's staining. The CCL3 receptor expression was evaluated by flow cytometry. The morphology and quantity of osteoblast were observed after exposure to CCL3.
  
  
  RESULTS
  Bone marrow osteoblasts from MM patients could be cultured in vitro and be identified by positive staining of alkaline phosphatase and Von Kossa's. MM-derived osteoblasts expressed higher levels of CCR1 (74.48 ± 7.31)%, compared with normal controls (48.35 ± 8.81)%. Calcium deposition of osteoblasts after exposure to CCL3 was less than that of controls.
  
  
  CONCLUSIONS
  Bone marrow osteoblasts could be cultured in vitro from MM Patients. CCL3 may contribute to the development of myeloma bone disease.
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Sijie
authors: S
authors: Zhao
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Rong
authors: R
authors: Fu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Hui
authors: H
authors: Liu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Yihao
authors: Y
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Lijuan
authors: L
authors: Li
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Chunyan
authors: C
authors: Liu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Tian
authors: T
authors: Zhang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Shaoxue
authors: S
authors: Ding
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Shan
authors: S
authors: Gao
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Erbao
authors: E
authors: Ruan
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Wen
authors: W
authors: Qu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Huaquan
authors: H
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Xiaoming
authors: X
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Yuhong
authors: Y
authors: Wu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Jia
authors: J
authors: Song
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Hong
authors: H
authors: Liu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Jing
authors: J
authors: Guan
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Zonghong
authors: Z
authors: Shao
mesh_terms: D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D054405:Chemokine CCL3 / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000473:pathology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D054389:Receptors, CCR1 / Q000378:metabolism; D055815:Young Adult
chemical_list: C515300:CCR1 protein, human; D054405:Chemokine CCL3; D054389:Receptors, CCR1