title: Preparation and immunogenicity of conjugate based on hydrazine-treated lipopolysaccharide antigen of Vibrio cholerae O139.
abstract: A glycoconjugate construct was based on attachment of V. cholerae O139 hydrazine-treated lipopolysaccharide (LPS) to carboxylated bovine serum albumin (CBSA) via its amino group. The immunological properties of the glycoconjugate were tested using BALB/c mice, injected subcutaneously without any adjuvant three times at 2 weeks interval. The immunogenicity of the conjugate was estimated by enzyme-linked immunosorbent assay, testing of anti-LPS IgG, IgM, and IgA antibodies. The conjugate elicited a statistically significant increase of LPS-specific IgG levels in mice (p < 0.001). The specific anti-LPS IgG and IgA response after the second booster dose was significantly higher compared with reference and unconjugated detoxified LPS response. Antibodies elicited by the dLPS-CBSA conjugate were vibriocidal.
authors: a Institute of Chemistry , Slovak Academy of Sciences , Bratislava , Slovak Republic.
authors: Anna
authors: A
authors: Fleischhackerová
authors: Pavol
authors: P
authors: Farkaš
authors: Alžbeta
authors: A
authors: Cížová
authors: Slavomír
authors: S
authors: Bystrický
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000378:metabolism; D000942:Antigens, Bacterial / Q000276:immunology*; D022121:Cholera Vaccines / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006834:Hydrazines / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018074:Vaccines, Conjugate / Q000737:chemistry / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology; D041242:Vibrio cholerae O139 / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D022121:Cholera Vaccines; D006834:Hydrazines; D008070:Lipopolysaccharides; D018074:Vaccines, Conjugate; D012710:Serum Albumin, Bovine; C029424:hydrazine
keywords: antibodies; cholera; lipopolysaccharide; vaccine; vibriocidal