title: The in vitro effects of sodium salicylate on von Willebrand factor and C-reactive protein production by endothelial cells.
abstract: OBJECTIVE
  The role of inflammatory and endothelial dysfunction markers in the atherogenic process has been well recognized. The data have made both C-reactive protein (CRP) and von Willebrand factor (vWF) promising targets for the cardiovascular disease research and drug development. Inhibition of CRP and vWF synthesis, therefore, might be a potential therapeutic strategy.
  
  
  METHODS
  The effect of sodium salicylate on vWF production by human umbilical vein endothelial cells (HUVECs) using enzyme-linked immunosorbent Assays (ELISA) and real-time PCR was examined. In addition, small interfering RNA (siRNA) against NF-κB was used to investigate the existence of a role for this signaling pathway.
  
  
  RESULTS
  Our findings demonstrated that sodium salicylate decreased vWF, but not CRP production at both mRNA and protein levels significantly and this might not occur via nuclear transcription factor (NF-κB) inhibition.
  
  
  CONCLUSIONS
  Our results indicated a further rationalization of the effects of sodium salicylate on atherothrombotic events by attenuation of vWF production.
authors: Hematology Department, School of Allied Medical Science, Iran University of Medical Science and Health Services, Tehran, Iran, ms1989ir@yahoo.com.
authors: Minoo
authors: M
authors: Shahidi
authors: Mahmood
authors: M
authors: Barati
authors: Parisa
authors: P
authors: Hayat
authors: Behnaz
authors: B
authors: Tavasoli
authors: Masoomeh
authors: M
authors: Bakhshayesh
mesh_terms: D002097:C-Reactive Protein / Q000187:drug effects* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D012980:Sodium Salicylate / Q000494:pharmacology*; D014841:von Willebrand Factor / Q000187:drug effects* / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014841:von Willebrand Factor; D002097:C-Reactive Protein; D012980:Sodium Salicylate