title: Bioactivity-guided chemical analysis of Melia azedarach L. (Meliaceae), displaying antidiabetic activity.
abstract: One new Euphane-type triterpenoid 3β-hydroxytirucalla-5, 24-dien-21-oic acid (1), and ten known compounds (2-11) were isolated from Melia azedarach L. through bioassay-guided chemical analysis. The structures of the isolated compounds were established by means of 1D and 2D NMR spectroscopic ((1)H, (13)C, DEPT, COSY, HSQC and HMBC) and MS spectral analyses. All the fractions and isolated pure compounds were evaluated for antidiabetic activity by determining their inhibitory effects on PTP-1B enzyme as well as glucose uptake stimulation in C2Cl2 myoblasts cells. Compounds 4 and 7 showed significant in vitro PTP-1B inhibitory activity with 69.2 and 66.8% inhibition at 10 μg/ml concentrations respectively.
authors: Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal) 246174, Uttarakhand, India.
authors: Mohammad Faheem
authors: MF
authors: Khan
authors: Biochemistry Division, CSIR-Central Drug Research Institute, CSIR, Lucknow 226031, India.
authors: Arun Kumar
authors: AK
authors: Rawat
authors: Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal) 246174, Uttarakhand, India.
authors: Bhawna
authors: B
authors: Pawar
authors: Biochemistry Division, CSIR-Central Drug Research Institute, CSIR, Lucknow 226031, India.
authors: Sudeep
authors: S
authors: Gautam
authors: Biochemistry Division, CSIR-Central Drug Research Institute, CSIR, Lucknow 226031, India.
authors: Arvind Kumar
authors: AK
authors: Srivastava
authors: Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal) 246174, Uttarakhand, India. Electronic address: devendra_negi@yahoo.com.
authors: Devendra Singh
authors: DS
authors: Negi
mesh_terms: D000818:Animals; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry*; D031586:Melia azedarach / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004791:Enzyme Inhibitors; D007004:Hypoglycemic Agents; D014315:Triterpenes; C516607:PTPN1 protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1
keywords: 3β-Hydroxytirucalla-5,24-Dien-21-oic acid; Glucose uptake stimulation; Melia azedarach; PTP-1B inhibition