title: Improved fibroblast functionalities by microporous pattern fabricated by microelectromechanical systems.
abstract: Fibroblasts, which play an important role in biological seal formation and maintenance, determine the long-term success of percutaneous implants. In this study, well-defined microporous structures with micropore diameters of 10-60 µm were fabricated by microelectromechanical systems and their influence on the fibroblast functionalities was observed. The results show that the microporous structures with micropore diameters of 10-60 µm did not influence the initial adherent fibroblast number; however, those with diameters of 40 and 50 µm improved the spread, actin stress fiber organization, proliferation and fibronectin secretion of the fibroblasts. The microporous structures with micropore diameters of 40-50 µm may be promising for application in the percutaneous part of an implant.
authors: State Key Laboratory of Military Stomatology, Department of Oral Implant, School of Stomatology, the Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China. weihongbo101@gmail.com.
authors: Hongbo
authors: H
authors: Wei
authors: State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, the Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China. zhaolingzhou1983@hotmail.com.
authors: Lingzhou
authors: L
authors: Zhao
authors: State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710048, China. bangdao2008@sina.com.
authors: Bangdao
authors: B
authors: Chen
authors: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China. shizhu1976@gmail.com.
authors: Shizhu
authors: S
authors: Bai
authors: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China. zhaoym@fmmu.edu.cn.
authors: Yimin
authors: Y
authors: Zhao
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation / Q000379:methods*; D016062:Porosity; D019736:Prostheses and Implants
chemical_list: D005353:Fibronectins