title: Investigation of toxin gene diversity and antimicrobial resistance ofClostridium difficilestrains.
abstract: The incidence ofClostridium difficileinfection (CDI) has been previously reported in a number of studies. However, data collected from the Chinese population is limited. In the present study, the diversity of the toxin genes,tcdAandtcdB,of 57Clostridium difficile(C. difficile) isolates from a Chinese population were investigated by polymerase chain reaction (PCR) (38 A+B+, 14 A-B+and 5 A-B-). Quantitative PCR was used to check the expression of these two genes and it was found that the genes were not expressed by all the strains. The absence oftcdAortcdBexpression in certain strains could be due to the lower expression oftcdDand the higher expression oftcdC, which are positive and negative regulators for these two toxin genes, respectively. In addition, the antimicrobial susceptibilities of 57 isolates were investigated. Therefore, these data would aid in the future prevention of CDI outbreaks and improve the understanding of the infection.
authors: Central Hospital of Taizhou City, Taizhou, Zhejiang 318000, P.R. China.
authors: Shanshan
authors: S
authors: Zhu
authors: Central Hospital of Taizhou City, Taizhou, Zhejiang 318000, P.R. China.
authors: Huaping
authors: H
authors: Zhang
authors: Central Hospital of Taizhou City, Taizhou, Zhejiang 318000, P.R. China.
authors: Xinsheng
authors: X
authors: Zhang
authors: Central Hospital of Taizhou City, Taizhou, Zhejiang 318000, P.R. China.
authors: Chao
authors: C
authors: Wang
authors: Central Hospital of Taizhou City, Taizhou, Zhejiang 318000, P.R. China.
authors: Guangming
authors: G
authors: Fan
authors: Central Hospital of Taizhou City, Taizhou, Zhejiang 318000, P.R. China.
authors: Weifeng
authors: W
authors: Zhang
authors: Central Hospital of Taizhou City, Taizhou, Zhejiang 318000, P.R. China.
authors: Gang
authors: G
authors: Sun
authors: Central Hospital of Taizhou City, Taizhou, Zhejiang 318000, P.R. China.
authors: Huihong
authors: H
authors: Chen
authors: Central Hospital of Taizhou City, Taizhou, Zhejiang 318000, P.R. China.
authors: Liming
authors: L
authors: Zhang
authors: Central Hospital of Taizhou City, Taizhou, Zhejiang 318000, P.R. China.
authors: Zhaoyun
authors: Z
authors: Li
keywords: Clostridium difficile infection; antimicrobial susceptibilities; tcdA; tcdB; toxin genes