title: Sperm selection by Capripure(®) density-gradient centrifugation versus the dextran swim-up procedure in wild mountain ruminants.
abstract: This study compares the effectiveness of two methods of sperm selection - Capripure(®) density-gradient centrifugation (DGC) and dextran swim-up (DSU) - in semen samples from Iberian ibex (Capra pyrenaica) and European mouflon (Ovis musimon). During the increasing photoperiod, Capripure(®) DGC improved the percentage of sperm with progressive motility (P<0.05) in ibexes, and selected 60.6% of the initial total number of spermatozoa contained in the ejaculate samples. In mouflon, Capripure(®) DGC selection was unaffected by photoperiod, had no influence on any sperm variable, and selected 47.8% of the initial total number of mouflon spermatozoa in ejaculate samples. Photoperiod had no influence on the effectiveness of DSU in either ibexes or mouflons. In the ibexes, DSU reduced (P<0.05) the percentage of sperm cells with morphological abnormalities, but only selected 11.3% of the initial total number of spermatozoa in ejaculate samples. In the mouflons, DSU had no significant influence on any sperm variable, and selected 27.8% of the initial total number. Capripure(®) DGC improved ibex and mouflon sperm motility (P<0.05) following 30min and 2h of post-centrifugation, stress-inducing incubation, respectively. In both ibexes and mouflon, sperm cells showing non-progressive motility were found after only 20 h of post-centrifugation incubation following Capripure(®) DGC selection. In conclusion, Capripure(®) DGC would seem a useful method for selecting the best spermatozoa from both ibex and mouflon ejaculates.
authors: Departamento de Reproducción Animal, INIA, 28040 Madrid, Spain. Electronic address: moreno@inia.es.
authors: J
authors: J
authors: Santiago-Moreno
authors: Departamento de Reproducción Animal, INIA, 28040 Madrid, Spain.
authors: M C
authors: MC
authors: Esteso
authors: Departamento de Reproducción Animal, INIA, 28040 Madrid, Spain.
authors: C
authors: C
authors: Castaño
authors: Departamento de Reproducción Animal, INIA, 28040 Madrid, Spain.
authors: A
authors: A
authors: Toledano-Díaz
authors: Departamento de Reproducción Animal, INIA, 28040 Madrid, Spain.
authors: E
authors: E
authors: Rodríguez
authors: Departamento de Reproducción Animal, INIA, 28040 Madrid, Spain.
authors: A
authors: A
authors: López-Sebastián
mesh_terms: D000818:Animals; D000835:Animals, Wild; D002469:Cell Separation / Q000379:methods / Q000662:veterinary*; D002499:Centrifugation, Density Gradient / Q000662:veterinary*; D003911:Dextrans / Q000737:chemistry*; D006041:Goats / Q000502:physiology*; D008297:Male; D012756:Sheep / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology
chemical_list: D003911:Dextrans
keywords: Density gradient centrifugation; Mouflon; Sperm purification; Swim-up techniques, Ibex