title: Zero-mode waveguide detection of flow-driven DNA translocation through nanopores.
abstract: We directly measure the flow-driven injection of DNA through nanopores at the level of single molecule and single pore using a modified zero-mode waveguide method. We observe a flow threshold independent of the pore radius, the DNA concentration, and length. We demonstrate that the flow injection of DNA in nanopores is controlled by an energy barrier as proposed in the de Gennes-Brochard suction model. Finally, we show that the height of the energy barrier is modulated by functionalizing the nanopores.
authors: Matiére et Systèmes Complexes, Université Paris Diderot & CNRS (UMR 7057), 75205 Paris Cedex 13, France.
authors: Thomas
authors: T
authors: Auger
authors: Laboratoire d'Analyse et de Modélisation pour la Biologie et l'Environnement, Université Évry-Val d'Essonne & CNRS (UMR 8587), 91025 Évry Cedex, France.
authors: Jérôme
authors: J
authors: Mathé
authors: Mechanobiology Institute, National University of Singapore & CNRS (UMI 3639), 5A Engineering Drive 1, Singapore 117411, Singapore.
authors: Virgile
authors: V
authors: Viasnoff
authors: Matiére et Systèmes Complexes, Université Paris Diderot & CNRS (UMR 7057), 75205 Paris Cedex 13, France.
authors: Gaëlle
authors: G
authors: Charron
authors: Matiére et Systèmes Complexes, Université Paris Diderot & CNRS (UMR 7057), 75205 Paris Cedex 13, France.
authors: Jean-Marc
authors: JM
authors: Di Meglio
authors: Matiére et Systèmes Complexes, Université Paris Diderot & CNRS (UMR 7057), 75205 Paris Cedex 13, France.
authors: Loïc
authors: L
authors: Auvray
authors: Matiére et Systèmes Complexes, Université Paris Diderot & CNRS (UMR 7057), 75205 Paris Cedex 13, France.
authors: Fabien
authors: F
authors: Montel
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D001583:Benzoxazoles / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D017022:Flow Injection Analysis / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry; D008956:Models, Chemical; D058608:Nanopores; D011806:Quinolinium Compounds / Q000737:chemistry; D013329:Structure-Activity Relationship; D013816:Thermodynamics
chemical_list: D001583:Benzoxazoles; D004279:DNA, Viral; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D011806:Quinolinium Compounds; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; D004247:DNA