title: The role of N-acetyltransferase 8 in mesenchymal stem cell-based therapy for liver ischemia/reperfusion injury in rats.
abstract: OBJECTIVE
  To evaluate the impact of mesenchymal stem cells (MSCs) against hepatic I/R injury and explore the role of N-acetyltransferase 8 (NAT8) in the process.
  
  
  METHODS
  We investigated the potential of injected MSCs systemically via the tail vein in healing injuried liver of the SD rat model of 70% hepatic I/R injury by measuring the biochemical and pathologic alterations. Subsequently, we evaluated the expression levels of NAT8 by western blotting in vivo. Concurrently, hydrogen peroxide (H2O2)-induced apoptosis in the human normal liver cell line L02 was performed in vitro to evaluate the protective effects of MSC conditioned medium (MSC-CM) on L02 cells. In addition, we downregulated and upregulated NAT8 expression in L02 cells and induced apoptosis by using H2O2 to study the protective role of NAT8.
  
  
  RESULTS
  MSCs implantation led to a significant reduced liver enzyme levels, an advanced protection in the histopathological findings of the acutely injured liver and a significantly lower percentage of TUNEL-positive cells, which were increased after I/R injury. In vitro assays, MSC-CM inhibited hepatocyte apoptosis induced by H2O2. Moreover, overexpression or downregulation of NAT8 prevented or aggravated hepatocyte apoptosis induced by H2O2, respectively.
  
  
  CONCLUSIONS
  MSC transplantation provides support to the I/R-injured liver by inhibiting hepatocellular apoptosis and stimulating NAT8 regeneration.
authors: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors: Jinqiu
authors: J
authors: Fu
authors: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors: Haiyan
authors: H
authors: Zhang
authors: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors: Yong
authors: Y
authors: Zhuang
authors: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors: Huan
authors: H
authors: Liu
authors: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors: Qing
authors: Q
authors: Shi
authors: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors: Dong
authors: D
authors: Li
authors: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors: Xiuli
authors: X
authors: Ju
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000737:chemistry*; D004195:Disease Models, Animal; D015536:Down-Regulation; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000201:enzymology / Q000473:pathology / Q000628:therapy*
chemical_list: D017077:Culture Media, Conditioned; D006861:Hydrogen Peroxide; D000123:Acetyltransferases