title: An enzyme-linked chemiluminescent immunoassay developed for detection of Butocarboxim from agricultural products based on monoclonal antibody.
abstract: In this study, four different haptens around the oxime moiety of Butocarboxim were designed and synthesised. Two of the haptens were conjugated with bovine serum albumin (BSA) to serve as the immunogen and all the haptens were conjugated with ovalbumin (OVA) for the coating antigen. The anti-Butocarboxim monoclonal antibody (Mab) was selected based on eight immunogen/coating antigen combinations. The first enzyme-linked chemiluminescent immunoassay (ELCIA) for determining Butocarboxim in agricultural products was developed. Under the optimised conditions, the detection limit for the ELCIA was 20 ng·mL(-1) and the linear range was 27-2700 ng·mL(-1). Analyte recoveries for extracts of spiked agricultural (apple and greengrocery) products and tap water ranged from 97.18% to 107.00%. The developed immunoassay has great potential to be developed as a test kit offering a simple and cost-effective approach (such as lateral flow test strip) for screening purposes and evaluating environmental exposure to Butocarboxim.
authors: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Qingkui
authors: Q
authors: Fang
authors: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Limin
authors: L
authors: Wang
authors: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Xiude
authors: X
authors: Hua
authors: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Yulong
authors: Y
authors: Wang
authors: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Suyan
authors: S
authors: Wang
authors: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Qi
authors: Q
authors: Cheng
authors: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Jia
authors: J
authors: Cai
authors: Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China; Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, PR China. Electronic address: fqliu20011@sina.com.
authors: Fengquan
authors: F
authors: Liu
mesh_terms: D000383:Agriculture / Q000379:methods*; D000448:Aldicarb / Q000031:analogs & derivatives* / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007118:Immunoassay / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; C044644:butocarboxime; D000448:Aldicarb
keywords: Butocarboxim; Enzyme-linked chemiluminescent immunoassay; Monoclonal antibody; Oxime moiety