title: A synthetic thiourea-based tripodal receptor that impairs the function of human first trimester cytotrophoblast cells.
abstract: A synthetic tripodal-based thiourea receptor (PNTTU) was used to explore the receptor/ligand binding affinity using CTB cells. The human extravillous CTB cells (Sw.71) used in this study were derived from first trimester chorionic villus tissue. The cell proliferation, migration and angiogenic factors were evaluated in PNTTU-treated CTB cells. The PNTTU inhibited the CTBs proliferation and migration. The soluble fms-like tyrosine kinase-1 (sFlt-1) secretion was increased while vascular endothelial growth factor (VEGF) was decreased in the culture media of CTB cells treated with ≥1 nM PNTTU. The angiotensin II receptor type 2 (AT2) expression was significantly upregulated in ≥1 nM PNTTU-treated CTB cells in compared to basal; however, the angiotensin II receptor, type 1 (AT1) and vascular endothelial growth factor receptor 1 (VEGFR-1) expression was downregulated. The anti-proliferative and anti-angiogenic effect of this compound on CTB cells are similar to the effect of CTSs. The receptor/ligand affinity of PNTTU on CTBs provides us the clue to design a potent inhibitor to prevent the CTS-induced impairment of CTB cells.
authors: Department of Obstetrics & Gynecology, Texas A&M Health Science Center College of Medicine/Scott & White Hospital, Temple, TX 76508, USA. DHORVAT@sw.org.
authors: Darijana
authors: D
authors: Horvat
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 39217, USA. maryam.emami_khansari@students.jsums.edu.
authors: Maryam Emami
authors: ME
authors: Khansari
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 39217, USA. avijit.pramanik@jsums.edu.
authors: Avijit
authors: A
authors: Pramanik
authors: Department of Pediatrics, Texas A&M Health Science Center College of Medicine/Scott & White Hospital, Temple, TX 76508, USA. mbeeram@sw.org.
authors: Madhava R
authors: MR
authors: Beeram
authors: Department of Obstetrics & Gynecology, Texas A&M Health Science Center College of Medicine/Scott & White Hospital, Temple, TX 76508, USA. mbeeram@sw.org.
authors: Thomas J
authors: TJ
authors: Kuehl
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS 39217, USA. alamgir.hossain@jsums.edu.
authors: Md Alamgir
authors: MA
authors: Hossain
authors: Department of Obstetrics & Gynecology, Texas A&M Health Science Center College of Medicine/Scott & White Hospital, Temple, TX 76508, USA. mnuddin@sw.org.
authors: Mohammad Nasir
authors: MN
authors: Uddin
mesh_terms: D002460:Cell Line; D005260:Female; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000378:metabolism; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism; D044139:Receptor, Angiotensin, Type 2 / Q000378:metabolism; D062165:Receptors, Artificial / Q000378:metabolism*; D013890:Thiourea / Q000031:analogs & derivatives* / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism
chemical_list: D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D062165:Receptors, Artificial; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1; D013890:Thiourea