title: Tumor regression by CD4 T-cells primed with dendritic/tumor fusion cell vaccines.
abstract: OBJECTIVE
  Vaccination with fusions of dendritic cells (DCs) and mucin-1 (MUC1)-positive tumor cells (FC/MUC1) induces MUC1-specific antitumor immunity. However, little is known about the function of Cluster of Differentiation (CD)4 T-cells primed with FC/MUC1 in MUC1 transgenic (MUC1.Tg) mice.
  
  
  METHODS
  CD4 T-cells primed with FC/MUC1 were analyzed by flow cytometry. Antitumor immunity by adoptive transfer of primed CD4 T-cells in Rag2(-/-) mice was assessed.
  
  
  RESULTS
  The effector and memory T-cells generated with FC/MUC1 were crucial to maintenance of long-term antitumor immunity. MUC1-8-mer peptide SAPDTRPA presented by FC/MUC1 was recognized by CD4 and CD8 T-cells. A subset of primed CD4 T-cells possessed cytotoxicity to lyse major histocompatibility complex (MHC) class I and MUC1 positive tumor cells. Interestingly, adoptive transfer of primed CD4 T-cells prevented lung metastasis in Rag2(-/-) mice.
  
  
  CONCLUSIONS
  CD4 T-cells primed by FC/MUC1 play direct role in antitumor immunity.
authors: Department of Internal Medicine, The Jikei University School of Medicine, Chiba, Japan shigeo_koido@jikei.ac.jp jgong@bu.edu.
authors: Shigeo
authors: S
authors: Koido
authors: Department of Urology, Mitsui Memorial Hospital, Tokyo, Japan.
authors: Yutaka
authors: Y
authors: Enomoto
authors: College of Health and Biomedicine, Centre for Chronic Disease Prevention and Management, Victoria University, Melbourne, Australia.
authors: Vasso
authors: V
authors: Apostolopoulos
authors: Department of Medicine, Boston University School of Medicine, Boston, MA, U.S.A. shigeo_koido@jikei.ac.jp jgong@bu.edu.
authors: Jianlin
authors: J
authors: Gong
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D017951:Antigen Presentation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology*; D002459:Cell Fusion; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018396:Mucin-1 / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000628:therapy*
chemical_list: D019496:Cancer Vaccines; D018396:Mucin-1; C577056:muc1 protein, mouse
keywords: CD4 T-cell; MUC1; cancer vaccine; dendritic cell; epitope; fusion cell