title: Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase.
abstract: Sodium dodecyl sulfate (SDS) is a widely used anionic surfactant in industry and research settings, and is known to have a detrimental effect to the environment. The pathway of SDS degradation by bacteria is initiated by an alkylsulfatase and the oxidized product, 1-dodecanoic acid, subsequently enters into the β-oxidation pathway and is used as a carbon source. In this work, we solved the crystal structure of Escherichia coli uncharacterized protein YjcS and identified that it belongs to the Type III alkylsulfatase with a signal peptide (residues 1-29) at the N terminus. YjcS hydrolyzed SDS and the double mutant D184N-H185A located in the conserved HXHXDH catalytic motif abolished this activity.
authors: School of Life Sciences, University of Science and Technology of China, Hefei, 230027, People's Republic of China.
authors: Yajing
authors: Y
authors: Liang
authors: Zengqiang
authors: Z
authors: Gao
authors: Yuhui
authors: Y
authors: Dong
authors: Quansheng
authors: Q
authors: Liu
mesh_terms: D020816:Amino Acid Motifs; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D017434:Protein Structure, Tertiary; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism; D013429:Sulfatases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D012967:Sodium Dodecyl Sulfate; D013429:Sulfatases; C019244:alkylsulfatase
keywords: X-ray crystallography; alkylsulfatase; hydrolase; metallo-β-lactamase; metalloenzyme