title: Antitumor properties of two traditional aromatic rice genotypes (Kalijira and Chinigura).
abstract: OBJECTIVE
  Methanol extract of bran and unpolished grain of two traditional aromatic rice genotypes viz. Kalijira and Chinigura were assayed for their activity on the growth and initiation of crown-gall tumors on potato disks.
  
  
  METHODS
  Three Agrobacterium tumefaciens (A. tumefaciens) strain AtSl0105, AtTa0112, and AtAc0114 were used as the tumor forming agent. Collected rice was separated to bran and unpolished grain by different milling processes and made into fine powder before extracting using methanol. Antitumor assay of plant extracts was performed according to standard potato disc bioassay. Disc diffusion assay (Kirby-Bauer Method) was used to screen A. tumefaciens sensitivity test.
  
  
  RESULTS
  The results demonstrated a high correlation between the ability of aromatic rice to inhibit the initiation and growth of crown-gall tumors on potato disks. Maximum tumor inhibitions were observed against the strain AtSl0105 by Kalijira bran (73.91%) and Chinigura bran (69.56%). Both unpolished grains showed significant effect (Kalijira 57.43%, Chinigura 55.53%) to inhibit the tumor.
  
  
  CONCLUSIONS
  It can be concluded that aromatic rice (Kalijira and Chinigura) might be a potential source of antitumor agent that can be used for further drug development for tumor treatment.
authors: Plant Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh.
authors: Mohammad Abdul
authors: MA
authors: Mannan
authors: Plant Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh.
authors: Tushar Chandra
authors: TC
authors: Sarker
authors: Plant Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh.
authors: Ahmad Humayan
authors: AH
authors: Kabir
authors: Plant Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh.
authors: Mostafizur
authors: M
authors: Rahman
authors: Plant Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh.
authors: Mohammad Firoz
authors: MF
authors: Alam
keywords: Antitumor activity; Traditional rice; Tumor inhibition; Unpolished grain