title: Molecularly imprinted protein recognition thin films constructed by controlled/living radical polymerization.
abstract: We demonstrated the synthesis of molecularly imprinted polymers (MIPs) with binding affinity toward a target protein, ribonuclease A (RNase) by atom transfer radical polymerization (ATRP) of acrylic acid, acrylamide, and N,N'-methylenebisacrylamide in the presence of RNase. The binding activity of the MIPs was evaluated by surface plasmon resonance (SPR) of the MIP thin layers prepared on the gold-coated sensor chips. The MIPs prepared by ATRP (MIP-ATRP) had a binding affinity toward RNase with larger binding amount compared to MIPs prepared by conventional free radical polymerization methods (MIP-RP). Moreover, protein selectivity was evaluated using reference proteins (cytochrome c, myoglobin, and α-lactalbumin) and was confirmed in MIP-ATRP of optimum film thickness determined experimentally to be 15-30 nm; however, protein selectivity was not achieved in all MIP-RP. We have shown that ATRP is powerful technique for preparing protein recognition materials by molecular imprinting.
authors: Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
authors: Shogo
authors: S
authors: Sasaki
authors: Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
authors: Tooru
authors: T
authors: Ooya
authors: Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
authors: Yukiya
authors: Y
authors: Kitayama
authors: Graduate School of Engineering, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan. Electronic address: takeuchi@gold.kobe-u.ac.jp.
authors: Toshifumi
authors: T
authors: Takeuchi
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000178:Acrylamides / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D006046:Gold; D054802:Molecular Imprinting; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012260:Ribonucleases / Q000378:metabolism; D020349:Surface Plasmon Resonance
chemical_list: D000178:Acrylamides; D000179:Acrylates; D005609:Free Radicals; D011108:Polymers; D011506:Proteins; D020106:Acrylamide; D006046:Gold; D012260:Ribonucleases; C021221:N,N'-methylenebisacrylamide; C036658:acrylic acid
keywords: Controlled/living radical polymerization; Molecular imprinting; Molecular recognition; Proteins; Quartz crystal microbalance; Surface plasmon resonance