title: Reduced nasal transport of insulin-like growth factor-1 to the mouse cerebrum with olfactory bulb resection.
abstract: Although the olfactory nerve is involved in nasal transport of insulin-like growth factor-1 (IGF-1) to the brain, to our knowledge there have been no direct assessments of the effects of olfactory nerve damage on this transport. To determine whether olfactory bulb resection resulted in reduced transport of nasally administered human recombinant IGF-1 (hIGF-1) to the cerebrum, we measured the uptake of nasally administered iodine-125 hIGF-1 ((125)I-hIGF-1) in the cerebrum as a percentage of that in the blood in male ICR mice subjected to left olfactory bulb resection (model mice) and in sham-operated male ICR mice (control mice). Phosphorylated extracellular signal-regulated kinase (ERK) 1/2 (Thr202/Tyr204)/(Thr185/Tyr187) as a percentage of total ERK 1/2 in the left cerebrum was also assessed by using enzyme-linked immunosorbent assay after nasal administration of hIGF-1. Uptake of nasally administered (125)I-hIGF-1 in the cerebrum as a percentage of that in the blood was significantly lower in the model group than in the control group 30min after nasal administration of hIGF-1. Unilateral olfactory bulb resection prevented nasally administered hIGF-1 from increasing the phosphorylation of ERK 1/2 in the mouse cerebrum in vivo. These findings suggest that olfactory bulb damage reduces nasal transport of hIGF-1 to the brain in vivo.
authors: Department of Otorhinolaryngology, Kanazawa Medical University, 1-1 Daigaku, Uchinadamachi, Kahokugun, Ishikawa 920-0293, Japan and shigah@kanazawa-med.ac.jp.
authors: Hideaki
authors: H
authors: Shiga
authors: Department of Quantum Medical Technology, Graduate School of Medical Science, Kanazawa University, Ishikawa, Japan.
authors: Mikiya
authors: M
authors: Nagaoka
authors: Department of Quantum Medical Technology, Graduate School of Medical Science, Kanazawa University, Ishikawa, Japan.
authors: Kohshin
authors: K
authors: Washiyama
authors: Department of Otorhinolaryngology, Kanazawa Medical University, 1-1 Daigaku, Uchinadamachi, Kahokugun, Ishikawa 920-0293, Japan and.
authors: Junpei
authors: J
authors: Yamamoto
authors: Department of Otorhinolaryngology, Kanazawa Medical University, 1-1 Daigaku, Uchinadamachi, Kahokugun, Ishikawa 920-0293, Japan and.
authors: Kentaro
authors: K
authors: Yamada
authors: Department of Otorhinolaryngology, Kanazawa Medical University, 1-1 Daigaku, Uchinadamachi, Kahokugun, Ishikawa 920-0293, Japan and.
authors: Takuya
authors: T
authors: Noda
authors: Department of Otorhinolaryngology, Kanazawa Medical University, 1-1 Daigaku, Uchinadamachi, Kahokugun, Ishikawa 920-0293, Japan and.
authors: Masayuki
authors: M
authors: Harita
authors: Department of Quantum Medical Technology, Graduate School of Medical Science, Kanazawa University, Ishikawa, Japan.
authors: Ryohei
authors: R
authors: Amano
authors: Department of Otorhinolaryngology, Kanazawa Medical University, 1-1 Daigaku, Uchinadamachi, Kahokugun, Ishikawa 920-0293, Japan and.
authors: Takaki
authors: T
authors: Miwa
mesh_terms: D000818:Animals; D001692:Biological Transport; D054022:Cerebrum / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009297:Nasal Mucosa / Q000378:metabolism*; D009830:Olfactory Bulb / Q000601:surgery*; D009832:Olfactory Nerve / Q000502:physiology / Q000601:surgery
chemical_list: D007334:Insulin-Like Growth Factor I
keywords: extracellular signal–regulated kinase 1/2; insulin-like growth factor-1; olfactory bulb resection; olfactory transport