title: Role of the ANKMY2-FKBP38 axis in regulation of the Sonic hedgehog (Shh) signaling pathway.
abstract: Sonic hedgehog (Shh) is a secreted morphogen that controls the patterning and growth of various tissues in the developing vertebrate embryo, including the central nervous system. Ablation of the FK506-binding protein 38 (FKBP38) gene results in activation of the Shh signaling pathway in mouse embryos, but the molecular mechanism by which FKBP38 suppresses Shh signaling has remained unclear. With the use of a proteomics approach, we have now identified ANKMY2, a protein with three ankyrin repeats and a MYND (myeloid, Nervy, and DEAF-1)-type Zn(2+) finger domain, as a molecule that interacts with FKBP38. Co-immunoprecipitation analysis confirmed that endogenous FKBP38 and ANKMY2 interact in the mouse brain. Depletion or overexpression of ANKMY2 resulted in down- and up-regulation of Shh signaling, respectively, in mouse embryonic fibroblasts. Furthermore, combined depletion of both FKBP38 and ANKMY2 attenuated Shh signaling in these cells, suggesting that ANKMY2 acts downstream of FKBP38 to activate the Shh signaling pathway. Targeting of the zebrafish ortholog of mouse Ankmy2 (ankmy2a) in fish embryos with an antisense morpholino oligonucleotide conferred a phenotype reflecting loss of function of the Shh pathway, suggesting that the regulation of Shh signaling by ANKMY2 is conserved between mammals and fish. Our findings thus indicate that the FKBP38-ANKMY2 axis plays a key role in regulation of Shh signaling in vivo.
authors: From the Division of Cell Biology and.
authors: Shotaro
authors: S
authors: Saita
authors: From the Division of Cell Biology and smichi@bioreg.kyushu-u.ac.jp.
authors: Michiko
authors: M
authors: Shirane
authors: Division of Cell Regulation Systems, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan.
authors: Tohru
authors: T
authors: Ishitani
authors: Division of Cell Regulation Systems, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan.
authors: Nobuyuki
authors: N
authors: Shimizu
authors: From the Division of Cell Biology and.
authors: Keiichi I
authors: KI
authors: Nakayama
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D015398:Signal Transduction / Q000235:genetics*; D022021:Tacrolimus Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics
chemical_list: C000595262:ANKMY2 protein, mouse; D002352:Carrier Proteins; C471205:FKBP8 protein, human; D053823:Hedgehog Proteins; C465570:Shh protein, mouse; D015534:Trans-Activators; D022021:Tacrolimus Binding Proteins
keywords: Cell Signaling; Hedgehog Signaling Pathway; Mouse; Prolyl Isomerase; Proteomics; Zebrafish