title: Hypersensitive response toPotato virus Yin potato cultivar Sárpo Mira is conferred by theNy-Smiragene located on the long arm of chromosome IX.
abstract: Potato virus Y(PVY,Potyvirus) is the fifth most important plant virus worldwide in terms of economic and scientific impact. It infects members of the family Solanaceae and causes losses in potato, tomato, tobacco, pepper and petunia production. In potato and its wild relatives, two types of resistance genes against PVY have been identified. WhileRygenes confer symptomless extreme resistance,Nygenes cause a hypersensitive response visible as local necrosis that may also be able to prevent the virus from spreading under certain environmental conditions. The potato cultivar Sárpo Mira originates from Hungary and is highly resistant to PVY, although the source of this resistance remains unknown. We show that cv. Sárpo Mira reacts with a hypersensitive response leading to necrosis after PVYNTNinfection in detached leaf, whole plant and grafting assays. The hypersensitivity to PVYNTNsegregated amongst 140 individuals of tetraploid progeny of cvs. Sárpo Mira × Maris Piper in a 1:1 ratio, indicating that it was conferred by a single, dominant gene in simplex. Moreover, we identified five DNA markers linked to this trait and located the underlying locus (Ny-Smira) to the long arm of potato chromosome IX. This position corresponds to the location of theRychcandNy-1genes for PVY resistance. A simple PCR marker, located 1 cM from theNy-Smiragene, can be recommended for selection of PVY-resistant progeny of cv. Sárpo Mira.
authors: Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland.
authors: Iga
authors: I
authors: Tomczyńska
authors: The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH UK ; Cell and Molecular Sciences, James Hutton Institute, Dundee, DD2 5DA UK.
authors: Florian
authors: F
authors: Jupe
authors: Cell and Molecular Sciences, James Hutton Institute, Dundee, DD2 5DA UK.
authors: Ingo
authors: I
authors: Hein
authors: Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland.
authors: Waldemar
authors: W
authors: Marczewski
authors: Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland.
authors: Jadwiga
authors: J
authors: Sliwka
keywords: MAS; Mapping; Marker; PVY; Resistance; Solanum tuberosum