title: Radioresistance of cancer stem-like cell derived from canine tumours.
abstract: Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are a small subpopulation of cancer cells that are responsible for the initiation, recurrence and metastasis of cancer. We previously demonstrated that, using the Hoechst 33342 dye-based side population technique, CSCs/CICs in canine lung adenocarcinoma cell line exist. In this study, as CSCs/CICs are known to form spheres in anchorage-independent environment in vitro, we evaluated the stemness of spheroid cells derived from canine lung adenocarcinoma and osteosarcoma cells by expression of stemness markers, and investigated radioresistance. Spheroid cells showed greater expression of stemness markers Oct-4 and CD133 gene than those of adherent-cultured cells. In nude mouse xenograft models, spheroid cells showed higher tumourigenic ability than adherent-cultured cells. In addition, spheroid cells showed significantly resistant against radioactivity as compared with adherent-cultured cells. These results suggest that spheroid cells could possess stemness and provide a CSCs/CICs research tool to investigate CSCs/CICs of canine tumour cells.
authors: Laboratory of Biology, Azabu University School of Veterinary Medicine, Chuo-ku, Sagamihara, Japan.
authors: A
authors: A
authors: Tanabe
authors: Veterinary Teaching Hospital, Azabu University, Chuo-ku, Sagamihara, Japan.
authors: T
authors: T
authors: Deguchi
authors: Veterinary Teaching Hospital, Azabu University, Chuo-ku, Sagamihara, Japan.
authors: T
authors: T
authors: Sato
authors: Veterinary Teaching Hospital, Azabu University, Chuo-ku, Sagamihara, Japan.
authors: Y
authors: Y
authors: Nemoto
authors: Veterinary Teaching Hospital, Azabu University, Chuo-ku, Sagamihara, Japan.
authors: T
authors: T
authors: Maruo
authors: Veterinary Teaching Hospital, Azabu University, Chuo-ku, Sagamihara, Japan.
authors: H
authors: H
authors: Madarame
authors: Department of Veterinary Radiology, Azabu University School of Veterinary Medicine, Chuo-ku, Sagamihara, Japan.
authors: T
authors: T
authors: Shida
authors: Laboratory of Pathology, Azabu University School of Life and Environmental Science, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, Japan.
authors: Y
authors: Y
authors: Naya
authors: Laboratory of Pathology, Azabu University School of Life and Environmental Science, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, Japan.
authors: K
authors: K
authors: Ogihara
authors: Laboratory of Biology, Azabu University School of Veterinary Medicine, Chuo-ku, Sagamihara, Japan.
authors: H
authors: H
authors: Sahara
mesh_terms: D000230:Adenocarcinoma / Q000662:veterinary; D000818:Animals; D001562:Benzimidazoles; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D008175:Lung Neoplasms / Q000662:veterinary; D009369:Neoplasms; D014411:Neoplastic Stem Cells / Q000528:radiation effects*; D012516:Osteosarcoma / Q000662:veterinary; D011836:Radiation Tolerance / Q000528:radiation effects*; D018874:Spheroids, Cellular / Q000528:radiation effects
chemical_list: D001562:Benzimidazoles; C017807:bisbenzimide ethoxide trihydrochloride
keywords: cancer stem-like cell; canine; lung cancer; osteosarcoma; radioresistance; radiotherapy