title: Blockage of tissue factor ameliorates the lesion of laser-induced choroidal neovascularization in mice.
abstract: Choroidal neovascularization (CNV) occurs as a result of age-related macular degeneration (AMD) and causes severe vision loss among elderly patients. High expression of tissue factor (TF) was found in the retinas of AMD patients. In this study, we used anti-TF monoclonal antibody to test the effect of the TF blockage on experimental CNV induced by laser photocoagulation of retina in mice. Anti-TF monoclonal antibody or vehicle was administered intravitreally at day 1, 2 or 3 after laser application. We found that TF expression increased, and reached the peak at the 3rd week after the after laser application. Anti-TF monoclonal antibody can predominantly decrease the expression of TF, VEGF and F4/80, and reduced the area of CNV. Anti-TF monoclonal antibody also decreased incidence of CNV and leakage area. There were no pathological changes in the liver, heart, brain or kidney tissue. We conclude that TF plays an important role in CNV and anti-TF monoclonal antibody significantly decreases CNV in mouse model and anti-TF monoclonal antibody may have therapeutic potential in AMD.
authors: Eye Institute, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors: Li
authors: L
authors: Wang
authors: Department of Ophthalmology, Xinhua Hospital, Yili, China.
authors: Zhongkai
authors: Z
authors: Yang
authors: Eye Institute, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors: Ying
authors: Y
authors: Yu
authors: Eye Institute, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors: Chen
authors: C
authors: Cui
authors: Eye Institute, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China.
authors: Huaijin
authors: H
authors: Guan
authors: Eye Institute, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu, China. Electronic address: chenhuieye@126.com.
authors: Hui
authors: H
authors: Chen
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000627:therapeutic use*; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D002199:Capillary Permeability / Q000187:drug effects; D020256:Choroidal Neovascularization / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005451:Fluorescein Angiography; D007150:Immunohistochemistry; D007167:Immunotherapy; D058449:Intravitreal Injections; D017075:Laser Coagulation; D051379:Mice; D008810:Mice, Inbred C57BL; D060888:Real-Time Polymerase Chain Reaction; D013925:Thromboplastin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D042461:Vascular Endothelial Growth Factor A; C036763:monocyte-macrophage differentiation antigen; C467480:vascular endothelial growth factor A, mouse; D013925:Thromboplastin
keywords: anti-TF monoclonal antibody; choroidal neovascularization; mouse; tissue factor