title: Ex vivo expansion of murine and human hematopoietic stem cells.
abstract: Hematopoietic stem cells have the capacity to self-renew and give rise to the entirety of the mature blood and immune system throughout the lifespan of an organism. Here, we describe methods to isolate and culture murine bone marrow (BM) CD34(-)ckit(+)Sca1(+)Lineage(-) (CD34(-)KSL) hematopoietic stem cells (HSCs). We also describe a method to measure functional HSC content via the competitive repopulation assay. Furthermore, we summarize methods to isolate and culture human CD34(+)CD38(-)Lineage(-) cells which are enriched for human hematopoietic stem and progenitor cells.
authors: Division of Hematologic Malignancies and Cellular Therapy, Duke University Medical Center, 2400 Pratt St, 3961, Durham, NC, 27710, USA.
authors: Phuong L
authors: PL
authors: Doan
authors: John P
authors: JP
authors: Chute
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D005312:Fetal Blood / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D051379:Mice
chemical_list: D018952:Antigens, CD34; D051997:ADP-ribosyl Cyclase 1