title: No Variation at 29 Microsatellites in the Genome of Jatropha curcas.
abstract: Genetic variation in the genome of a given species is the basis for natural selection and genetic improvement through selective breeding. We applied 29 microsatellites located on 11 linkage groups to study genetic variation in 276 accessions of J. curcas collected from nine locations in five countries in South America, Asia and Africa to initiate a breeding program. To our surprise, we did not detect any genetic diversity at all 29 microsatellites loci. All the 276 accessions were homozygous at all loci and shared the same genotype at each locus, suggesting no microsatellite variation in the genome of Jatropha curcas. This result is quite unusual, and may have a profound influence on the breeding strategies and genome study of this species.
authors: 1. Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore. ; 2. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543 Singapore.
authors: Gen Hua
authors: GH
authors: Yue
authors: 1. Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore. ; 3. JOIL (S) Pte, Singapore, 117604 Singapore.
authors: Loong Chueng
authors: LC
authors: Lo
authors: 1. Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
authors: Fei
authors: F
authors: Sun
authors: 1. Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
authors: Su Ying
authors: SY
authors: Cao
authors: 3. JOIL (S) Pte, Singapore, 117604 Singapore.
authors: Cheng Xin
authors: CX
authors: Yi
authors: 3. JOIL (S) Pte, Singapore, 117604 Singapore.
authors: Yan
authors: Y
authors: Hong
authors: 4. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China.
authors: Wei Bang
authors: WB
authors: Sun
keywords: DNA markers; Jatropha; breeding.; epigenetics; genetic vitiation