title: Fibroblasts derived from long-lived insulin receptor substrate 1 null mice are not resistant to multiple forms of stress.
abstract: Reduced signalling through the insulin/insulin-like growth factor-1 signalling (IIS) pathway is a highly conserved lifespan determinant in model organisms. The precise mechanism underlying the effects of the IIS on lifespan and health is currently unclear, although cellular stress resistance may be important. We have previously demonstrated that mice globally lacking insulin receptor substrate 1 (Irs1(-/-) ) are long-lived and enjoy a greater period of their life free from age-related pathology compared with wild-type (WT) controls. In this study, we show that primary dermal fibroblasts and primary myoblasts derived from Irs1(-/-) mice are no more resistant to a range of oxidant and nonoxidant chemical stressors than cells derived from WT mice.
authors: Integrative and Environmental Physiology, Institute of Biology and Environmental Sciences, University of Aberdeen, Aberdeen, AB24 2TZ, UK.
authors: Melissa M
authors: MM
authors: Page
authors: Amy
authors: A
authors: Sinclair
authors: Ellen L
authors: EL
authors: Robb
authors: Jeffrey A
authors: JA
authors: Stuart
authors: Dominic J
authors: DJ
authors: Withers
authors: Colin
authors: C
authors: Selman
mesh_terms: D000818:Animals; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D055504:Insulin Receptor Substrate Proteins / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018384:Oxidative Stress / Q000502:physiology; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D055504:Insulin Receptor Substrate Proteins
keywords: IRS1; NRF2; aging; oxidative stress; stress resistance