title: L-arginine enhances arginine deiminase induced human lymphoma cell growth inhibition through NF-kBp65 and p53 expression in vitro.
abstract: OBJECTIVE
  Arginine deiminase (ADI) and L-arginine (L-Arg) can act as anti-tumor agents in-vitro and in-vivo. However, the mechanism of ADI and L-Arg as anti-tumor agents has not been clearly shown.
  
  
  METHODS
  With the goal of understanding the role of ADI and L-Arg in inhibition of cell growth, we used the Ramos human lymphoma cell line, which is known to be ADI-sensitive, and observed the p53 and NF-κBp65 protein expression after ADI and arginine treatment. After determining an optimal experimental ADI concentration (0.01 U/ml), we studied the effects of ADI treatment, when combined with different concentrations of L-arginine (control, ADI only, ADI with 10 mM/ml Arg, ADI with 30 mM/ml Arg, and ADI with 50 mM/ml Arg). An MTT assay was used to assess cell survival after treatment, Western blot analysis to determine the levels of the NF-κBp65, p53 and NO mediators and nitric oxide assays were used to determine nitrite levels.
  
  
  CONCLUSIONS
  L-arginine enhanced ADI-induced inhibited cell growth through expression of NF-κBp65 and p53 in a dose-dependent manner.
authors: Department of Pediatrics, Tongji Hospital Tongji University School of Medicine Shanghai, Republic of China. shuxiaoliang@vip.sina.com.
authors: X L
authors: XL
authors: Shu
authors: X L
authors: XL
authors: Liu
authors: J X
authors: JX
authors: Zhong
authors: J
authors: J
authors: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001120:Arginine / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006867:Hydrolases / Q000494:pharmacology*; D008223:Lymphoma / Q000188:drug therapy* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B; D016159:Tumor Suppressor Protein p53; D009569:Nitric Oxide; D001120:Arginine; D006867:Hydrolases; C019723:arginine deiminase