title: Performance of the Vitek 2 system software version 5.03 in the bacterial identification and antimicrobial susceptibility test: evaluation study of clinical and reference strains of Gram-positive cocci.
abstract: BACKGROUND
  The genera Enterococcus, Staphylococcus and Streptococcus are recognized as important Gram-positive human pathogens. The aim of this study was to evaluate the performance of Vitek 2 in identifying Gram-positive cocci and their antimicrobial susceptibilities.
  
  
  METHODS
  One hundred four isolates were analyzed to determine the accuracy of the automated system for identifying the bacteria and their susceptibility to oxacillin and vancomycin.
  
  
  RESULTS
  The system correctly identified 77.9% and 97.1% of the isolates at the species and genus levels, respectively. Additionally, 81.8% of the Vitek 2 results agreed with the known antimicrobial susceptibility profiles.
  
  
  CONCLUSIONS
  Vitek 2 correctly identified the commonly isolated strains; however, the limitations of the method may lead to ambiguous findings.
authors: Laboratório de Cocos Gram-Positivos, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
authors: Thiago Galvão da Silva
authors: TG
authors: Paim
authors: Laboratório de Cocos Gram-Positivos, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
authors: Vlademir Vicente
authors: VV
authors: Cantarelli
authors: Laboratório de Cocos Gram-Positivos, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
authors: Pedro Alves
authors: PA
authors: d'Azevedo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D057205:Automation, Laboratory; D006095:Gram-Positive Cocci / Q000145:classification / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000295:instrumentation* / Q000379:methods; D010068:Oxacillin / Q000494:pharmacology*; D015203:Reproducibility of Results; D012984:Software; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D014640:Vancomycin; D010068:Oxacillin