title: Intracellular antioxidant detoxifying effects of diosmetin on 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress through inhibition of reactive oxygen species generation.
abstract: The intracellular antioxidant activities of diosmetin were evaluated by cellular antioxidant activity (CAA) assay, 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced erythrocyte hemolysis assay and cupric chloride (CuCl2)-induced plasma oxidation assay. The results showed that diosmetin exhibits strong cellular antioxidant activity (EC50 = 7.98 μmol, CAA value = 58 μmol QE/100 μmol). It was also found that diosmetin treatment could effectively attenuate AAPH-induced erythrocyte hemolysis (91.0% inhibition at 100 μg/mL) and CuCl2-induced plasma oxidation through inhibition of intracellular reactive oxygen species (ROS) generation. Diosmetin could significantly restore AAPH-induced increase of intracelluar antioxidant enzyme (SOD, GPx, and CAT) activities to normal levels, as well as inhibit intracellular malondialdehyde (MDA) formation. Thus, the intracellular antioxidant detoxifying mechanism of diosmetin is associated with both nonenzymatic and enzymatic defense systems.
authors: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou 510640, China.
authors: Wenzhen
authors: W
authors: Liao
authors: Zhengxiang
authors: Z
authors: Ning
authors: Luying
authors: L
authors: Chen
authors: Qingyi
authors: Q
authors: Wei
authors: Erdong
authors: E
authors: Yuan
authors: Jiguo
authors: J
authors: Yang
authors: Jiaoyan
authors: J
authors: Ren
mesh_terms: D000328:Adult; D000578:Amidines / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000578:Amidines; D000975:Antioxidants; D005419:Flavonoids; D017382:Reactive Oxygen Species; C046728:2,2'-azobis(2-amidinopropane); C039602:diosmetin