title: Simultaneous analysis of nanoparticles and small molecules by high-performance liquid chromatography using a silica monolithic column.
abstract: A high-performance liquid chromatography method using a commercially available silica monolithic column for the simultaneous analysis of nanoparticles and small molecules was developed. The method uses the micrometer-sized flow-through pores and nanometer-sized mesopores of the monolithic column for separation: first, size separation of nanoparticles was performed by the micrometer-sized pores using the hydrodynamic mode, and then small molecules were separated by the nanometer-sized pores using the normal-phase mode. The method was used to evaluate and compare three existing methods for purifying nanoparticles and to analyse nanoparticle stability. The bimodal structure of the monolithic column is promising for the simultaneous separation of nanoparticles and small molecules.
authors: Graduate School of Pharmaceutical Sciences and GPLLI Program, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors: Naoki
authors: N
authors: Itoh
authors: Akira
authors: A
authors: Sano
authors: Tomofumi
authors: T
authors: Santa
authors: Masaru
authors: M
authors: Kato
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D053758:Nanoparticles / Q000032:analysis*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide