title: Transcription factor ABF-1 suppresses plasma cell differentiation but facilitates memory B cell formation.
abstract: Ag-primed B cells that result from an immune response can form either memory B cells or Ab-secreting plasma cells; however, the molecular machinery that controls this cellular fate is poorly understood. In this study, we show that activated B cell factor-1 (ABF-1), which encodes a basic helix-loop-helix transcriptional repressor, participates in this regulation. ABF-1 was prevalently expressed in purified memory B cells and induced by T follicular helper cell-mediated signals. ABF-1 expression declined by the direct repression of B lymphocyte-induced maturation protein-1 during differentiation. Ectopic expression of ABF-1 reduced the formation of Ab-secreting cells in an in vitro differentiation system of human memory B cells. Accordingly, knockdown of ABF-1 potentiates the formation of Ab-secreting cells. A transgenic mouse that expresses inducible ABF-1 in a B cell-specific manner was generated to demonstrate that the formation of germinal center and memory B cells was augmented by induced ABF-1 in an immune response, whereas the Ag-specific plasma cell response was dampened. This effect was associated with the ability of ABF-1 to limit cell proliferation. Together, our results demonstrate that ABF-1 facilitates formation of memory B cells but prevents plasma cell differentiation.
authors: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan; Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan;
authors: Yi-Kai
authors: YK
authors: Chiu
authors: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 112, Taiwan; and.
authors: I-Ying
authors: IY
authors: Lin
authors: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan;
authors: Shin-Tang
authors: ST
authors: Su
authors: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan; Institute of Immunology, National Taiwan University, Taipei 110, Taiwan.
authors: Kuan-Hsiung
authors: KH
authors: Wang
authors: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan;
authors: Shii-Yi
authors: SY
authors: Yang
authors: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 112, Taiwan; and.
authors: Dong-Yan
authors: DY
authors: Tsai
authors: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan;
authors: Yi-Ting
authors: YT
authors: Hsieh
authors: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan; Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 112, Taiwan; and Institute of Immunology, National Taiwan University, Taipei 110, Taiwan kuoilin@gate.sinica.edu.tw.
authors: Kuo-I
authors: KI
authors: Lin
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D049109:Cell Proliferation; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D051379:Mice; D008822:Mice, Transgenic; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology*; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology*
chemical_list: D014157:Transcription Factors