title: The effects of ABCG2 on the viability, proliferation and paracrine actions of kidney side population cells under oxygen-glucose deprivation.
abstract: Bcrp1/ABCG2 is exclusively expressed in side population (SP) cells, however, it has not been fully elucidated whether it has an impact on the viability, proliferation and paracrine actions in kidney SP cells under oxygen-glucose deprivation (OGD) followed by reoxygenation. In this study, we found that 2-h OGD did not injure SP cells (sub-lethal OGD) but induced SP cells proliferation 48 and 72 h after reoxygenation; whereas 4-h OGD markedly injured the cells (lethal OGD) and led to apoptosis 24-72 h after reoxygenation. Fumitremorgin C, an inhibitor of ABCG2, attenuated both the proliferation and viability of SP cells. Sub-lethal and lethal OGD induced the increase in the secretion of vascular endothelial growth factor, insulin-like growth factor 1, hepatocyte growth factor, and stromal cell-derived factor-1α in kidney SP cells, which was inhibited by Fumitremorgin C. Collectively, these findings provide evidence for a crucial role for the ABCG2 expression in the viability, proliferation and paracrine actions of kidney SP cells after OGD.
authors: 1. Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China; ; 2. State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an, 710032, China;
authors: Hong-Bao
authors: HB
authors: Liu
authors: 3. Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China;
authors: Qiu-Hong
authors: QH
authors: Meng
authors: 4. Department of Nephrology, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710032, China.
authors: De-Wei
authors: DW
authors: Du
authors: 4. Department of Nephrology, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710032, China.
authors: Ji-Feng
authors: JF
authors: Sun
authors: 3. Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China;
authors: Jian-Bo
authors: JB
authors: Wang
authors: 2. State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an, 710032, China;
authors: Hua
authors: H
authors: Han
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D001786:Blood Glucose / Q000378:metabolism*; D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D002470:Cell Survival / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D010100:Oxygen / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; C501412:Abcg2 protein, mouse; D001786:Blood Glucose; D010100:Oxygen
keywords: Apoptosis; Kidney; Paracrine; Proliferation; Side population; Stem cells; hypoxia; ischemia; oxygen-glucose deprivation.