title: Hepatocyte growth factor upregulates nexilin gene expression in cardiomyocytes via JNK pathway.
abstract: Hepatocyte growth factor (HGF) is a protective factor in myocardial injury, but its mechanisms of action have not yet been fully elucidated. Nexilin, which locates specifically to the Z-disc, is a novel Z-disc protein that enables the Z-discs to persistently withstand the extreme mechanical forces generated during muscle contraction. Therefore, we investigated the role of HGF in modulating nexilin expression in hypoxia-reoxygenation (H/R)-treated cardiomyocytes. We cultured neonatal cardiomyocytes and treated them with HGF. The mRNA and protein levels of nexilin were determined by RT-PCR and Western blotting. H/R treatment decreased nexilin mRNA expression and nexilin protein levels in cardiomyocytes. Furthermore, treatment with HGF upregulated nexilin expression and the JNK inhibitor SP600125 partly inhibited HGF-induced nexilin upregulation. In conclusion, our results suggest that ischemia-reperfusion injury may downregulate nexilin expression in cardiomyocytes, and HGF may exert its protective role during myocardial ischemic injury through upregulation of nexilin expression in cardiomyocytes.
authors: Department of Laboratory Medicine, Affiliated Hospital of Yanbian University, Yanji, China.
authors: C
authors: C
authors: Wang
authors: Department of Laboratory Medicine, Affiliated Hospital of Yanbian University, Yanji, China xinyou118@126.com.
authors: X
authors: X
authors: You
authors: Department of Laboratory Medicine, Affiliated Hospital of Yanbian University, Yanji, China.
authors: C
authors: C
authors: Jiang
authors: Department of Laboratory Medicine, Affiliated Hospital of Yanbian University, Yanji, China.
authors: C
authors: C
authors: Jin
authors: Core Laboratory, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: X
authors: X
authors: Meng
authors: Department of Cardiology, Affiliated Hospital of Yanbian University, Yanji, China.
authors: D
authors: D
authors: Ding
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000873:Anthracenes / Q000494:pharmacology; D002070:Butadienes / Q000494:pharmacology; D015687:Cell Hypoxia; D005419:Flavonoids / Q000494:pharmacology; D005786:Gene Expression Regulation; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008840:Microfilament Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D061251:Primary Cell Culture; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction
chemical_list: D000873:Anthracenes; D002070:Butadienes; D005419:Flavonoids; D008840:Microfilament Proteins; C116313:Nexn protein, rat; D009570:Nitriles; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; C113580:U 0126; C432165:pyrazolanthrone; D017228:Hepatocyte Growth Factor; D048031:JNK Mitogen-Activated Protein Kinases; D010100:Oxygen; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one