title: Monitoring Notch activation in cultured mammalian cells: transcriptional reporter assays.
abstract: Upon ligand binding, Notch activation and cleavage culminates in the expression of its target genes. Hence, the use of Notch-responsive promoters to drive reporter gene expression provides a flexible and robust approach for monitoring signaling activity. In this chapter, we present an overview of Notch transcriptional reporter assays and discuss different methods for ligand-independent and ligand-dependent activation of Notch in mammalian cells.
authors: Department of Developmental Biology, Washington University School of Medicine, Saint Louis, MO, 63110, USA, ilaganmg@wustl.edu.
authors: Ma Xenia G
authors: MX
authors: Ilagan
authors: Raphael
authors: R
authors: Kopan
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D006801:Humans; D011401:Promoter Regions, Genetic; D051880:Receptors, Notch / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation; D014162:Transfection / Q000379:methods*
chemical_list: D051880:Receptors, Notch