title: [Identification of histochrome metabolism products in urine for studying drug pharmacokinetics].
abstract: Histochrome is the medicinal form of echinochrome (2,3,5,6,8-pentahydroxy-7-ethyl-1,4-naphthoquinone). The drug pharmacodynamics and its relationship with metabolism, which was revealed in previous investigations, predetermined the aim of this work: to study excretion of echinochrome and its possible metabolites in urine of rats. Histochrome was introduced to Wistar rats (n = 10) subcutaneously in a dose of 10 mg/kg. Naphthoquinone derivatives were extracted from the acidified urine by ethyl acetate and analyzed by high performance liquid chromatography with UV detection (HPLC/UV) and mass-spectrometric detection (HPLC/MS). It was established that histochrome is completely metabolized in an organism and excreted by kidneys in the form of 3-methoxy-2,5,6,8-tetrahydroxy-7-ethyl-1,4-naphthoquinone and 2-metoxy-3,5,6,8-tetrahydroxy-7-ethyl-1,4-naphthoquinone.
authors: O S
authors: OS
authors: Talalaeva
authors: N P
authors: NP
authors: Mishchenko
authors: V M
authors: VM
authors: Briukhanov
authors: Ia F
authors: IaF
authors: Zverev
authors: V V
authors: VV
authors: Lampatov
authors: L G
authors: LG
authors: Dvornikova
mesh_terms: D000818:Animals; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009285:Naphthoquinones / Q000493:pharmacokinetics* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014556:Urine / Q000737:chemistry
chemical_list: D009285:Naphthoquinones; C103614:histochrome