title: Standardization of renal function evaluation in Wistar rats (Rattus norvegicus) from the Federal University of Juiz de Fora's colony.
abstract: BACKGROUND
  There is great interest in the use of animal models in the study of renal pathophysiology requires standardization of parameters.
  
  
  OBJECTIVE
  Standardize assessment of renal function in rats from in the Center for Reproductive Biology of Federal University of Juiz de Fora's colony.
  
  
  METHODS
  Thirty Wistar rats were used and performed measurements of creatinine (serum and urine), serum urea and proteinuria. Were evaluated: the urine collection interval in metabolic cages (24 hours or 12 hours), the need for 12-hour fast, the need of urine and serum deproteinization for creatinine measurement, need of serum deproteinization in animals with acute kidney injury to a spectrophotometer and ELISA, and the comparison of 24-hour proteinuria (PT 24 hours) with the protein/creatinine ratio (rP/C). Means were compared by the Student's t test, Pearson correlation, Bland-Altman plot for agreement and linear regression model to estimate PT 24 hours from rP/C.
  
  
  RESULTS
  The 24 hours urine output was greater than 12 hours, interfering with the creatinine clearance calculation. In the fasting group showed less water intake and lower urinary creatinine. There was great variability for the deproteinized whey and readings performed in the two devices were similar. There was a strong correlation between PT 24 hours and rP/C and the equation was generated: PT 24 hours = (8.6113 x rP/C) + 1.0869.
  
  
  CONCLUSIONS
  Was standardized: 24-hour urine collection without fasting. The deproteinization showed no benefit. The measurements were performed with spectrophotometer reliability. It generated a practical formula for estimating PT 24 hours through rP/C.
authors: Universidade Federal de Juiz de Fora.
authors: Bárbara Bruna Abreu
authors: BB
authors: de Castro
authors: Universidade Federal de Juiz de Fora.
authors: Fernando Antonio Basile
authors: FA
authors: Colugnati
authors: Universidade Federal de São Paulo.
authors: Marcos Antonio
authors: MA
authors: Cenedeze
authors: Universidade Federal de Juiz de Fora.
authors: Paulo Giovanni de Albuquerque
authors: PG
authors: Suassuna
authors: Universidade Federal de Juiz de Fora.
authors: Hélady Sanders
authors: HS
authors: Pinheiro
mesh_terms: D000818:Animals; D001938:Brazil; D004195:Disease Models, Animal; D007677:Kidney Function Tests / Q000592:standards*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014495:Universities; D016482:Urinalysis / Q000592:standards