title: Proteome phenotyping of ΔrelA mutants in Enterococcus faecalis V583.
abstract: The (p)ppGpp synthetase RelA contributes to stress adaptation and virulence in Enterococcus faecalis V583. A 2-dimensional electrophoresis proteomic analysis of 2 relA mutants, i.e., ΔrelA carrying a complete deletion of the relA gene, and ΔrelAsp that is deleted from only its 3' extremity, showed that 31 proteins were deregulated in 1 or both of these mutants. Mass spectrometry identification of these proteins showed that 10 are related to translation, including 5 ribosomal proteins, 3 proteins involved in translation elongation, and 2 proteins in tRNA synthesis; 14 proteins are involved in diverse metabolisms and biosynthesis (8 in sugar and energy metabolisms, 2 in fatty acid biosynthesis, 2 in amino acid biosynthesis, and 2 in nucleotide metabolism). Five proteins were relevant to the adaptation to different environmental stresses, i.e., SodA and a Dps family protein, 2 cold-shock domain proteins, and Ef1744, which is a general stress protein that plays an important role in the response to ethanol stress. The potential role of these proteins in the development of stress phenotypes associated with these mutations is discussed.
authors: a Unité de Recherche Risques Microbiens (U2RM), Equipe Stress Virulence, Université de Caen Basse-Normandie, 14032 Caen, France.
authors: Xue
authors: X
authors: Yan
authors: Aurélie
authors: A
authors: Budin-Verneuil
authors: Yanick
authors: Y
authors: Auffray
authors: Vianney
authors: V
authors: Pichereau
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D013293:Enterococcus faecalis / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D008025:Ligases / Q000235:genetics*; D053858:Metabolic Networks and Pathways; D009154:Mutation; D010641:Phenotype; D014176:Protein Biosynthesis; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D008025:Ligases; C028812:guanosine 3',5'-polyphosphate synthetases
keywords: general stress protein; guanine nucleotides; guanine nucléotides; mutants relA; proteome; protéine générale de stress; protéome; relA mutants