title: Aquimixticola soesokkakensis gen. nov., sp. nov., a novel lipolytic alphaproteobacterium isolated from the junction between the ocean and a freshwater spring, and reclassification of Roseovarius marinus as Pacificibacter marinus comb. nov. and emended description of the genus Pacificibacter.
abstract: A Gram-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated DSSK2-3(T), was isolated from the junction between the ocean and a freshwater spring at Jeju island, South Korea. Strain DSSK2-3(T) was found to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0% (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences revealed that strain DSSK2-3(T) joins the cluster comprising the type strains of Pacificibacter maritimus and Roseovarius marinus, with which it exhibited the highest sequence similarity values of 96.04 and 95.75%, respectively. Sequence similarities to the type strains of other recognized species were <95.74%. Strain DSSK2-3(T) was found to contain Q-10 as the predominant ubiquinone and C(18:1) ω7c as the major fatty acid. The polar lipid profile of strain DSSK2-3(T) was found to contain phosphatidylglycerol, an unidentified lipid and an unidentified aminolipid as major components, which distinguished the strain from those of phylogenetically related taxa. The DNA G+C content of strain DSSK2-3(T) was determined to be 60.8 mol%. On the basis of the phylogenetic data and the chemotaxonomic and other phenotypic properties, strain DSSK2-3(T) is considered to represent a new genus and species within the class Alphaproteobacteria, for which the name Aquimixticola soesokkakensis gen. nov., sp. nov. is proposed. The type strain of A. soesokkakensis is DSSK2-3(T) (=KCTC 42137(T) = CECT 8620(T)). In this study, it is also proposed that R. marinus should be reclassified as a member of the genus Pacificibacter and the description of the genus Pacificibacter is emended.
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors: Sooyeon
authors: S
authors: Park
authors: Chul-Hyung
authors: CH
authors: Kang
authors: Ji-Min
authors: JM
authors: Park
authors: Jung-Hoon
authors: JH
authors: Yoon
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride