title: Succinate production from CO₂-grown microalgal biomass as carbon source using engineered Corynebacterium glutamicum through consolidated bioprocessing.
abstract: The potential for production of chemicals from microalgal biomass has been considered as an alternative route for CO₂ mitigation and establishment of biorefineries. This study presents the development of consolidated bioprocessing for succinate production from microalgal biomass using engineered Corynebacterium glutamicum. Starch-degrading and succinate-producing C. glutamicum strains produced succinate (0.16 g succinate/g total carbon source) from a mixture of starch and glucose as a model microalgal biomass. Subsequently, the engineered C. glutamicum strains were able to produce succinate (0.28 g succinate/g of total sugars including starch) from pretreated microalgal biomass of CO₂-grown Chlamydomonas reinhardtii. For the first time, this work shows succinate production from CO₂ via sequential fermentations of CO₂-grown microalgae and engineered C. glutamicum. Therefore, consolidated bioprocessing based on microalgal biomass could be useful to promote variety of biorefineries.
authors: 1] Clean Energy Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, Republic of Korea [2] Department of Chemical and Biological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea.
authors: Jungseok
authors: J
authors: Lee
authors: 1] Department of Chemical and Biological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea [2] Green School, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea.
authors: Sang Jun
authors: SJ
authors: Sim
authors: Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, D-52425 Jülich, Germany.
authors: Michael
authors: M
authors: Bott
authors: 1] Clean Energy Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, Republic of Korea [2] Department of Clean Energy and Chemical Engineering, Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors: Youngsoon
authors: Y
authors: Um
authors: Department of Chemical and Biological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea.
authors: Min-Kyu
authors: MK
authors: Oh
authors: 1] Clean Energy Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, Republic of Korea [2] Green School, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, Republic of Korea [3] Department of Clean Energy and Chemical Engineering, Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea.
authors: Han Min
authors: HM
authors: Woo
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D010788:Photosynthesis; D013213:Starch / Q000378:metabolism; D013386:Succinates / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism*; D000516:alpha-Amylases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D013386:Succinates; D002245:Carbon Dioxide; D013213:Starch; D019802:Succinic Acid; D000516:alpha-Amylases; D005947:Glucose