title: Phytochemical analysis and bioactivity of the aerial parts of Abutilon theophrasti (Malvaceae), a medicinal weed.
abstract: Phytochemical investigations of aerial parts of Abutilon theophrasti yielded (6S,9R)-roseoside (1) and (6S,9S)-roseoside (2) which are new for the genus. The elucidation of the chemical structures was established by mass spectrometry, 1D and 2D NMR experiments. Although methanol extracts contained 48.5 ± 7.2 mg of caffeic acid equivalents and 15.87 ± 4.6 mg of quercetin equivalents, the antioxidant activity, as revealed by DPPH and ABTS assays, was of medium strength (EC50 of 306.2 ± 16.3 and 394.3 ± 14.8 μg/mL, respectively). A. theophrasti extract inhibits soybean 5-LOX with IC50 value 2.89 ± 0.2 mg/mL. The cytotoxicity of the methanol extract against MCF-7, CCRF-CEM and CEM/ADR5000 cancer cells resulted in IC50 values of 505.8 ± 34.7 μg/mL for MCF-7, 75.6 ± 7.1 μg/mL for CCRF-CEM, and 89.5 ± 13.4 μg/mL for CEM/ADR 5000 cells.
authors: a Institute of the Chemistry of Plant Substances AS RUz , Mirzo Ulugbek Str 77, Tashkent , 100170 , Uzbekistan.
authors: N Z
authors: NZ
authors: Mamadalieva
authors: F
authors: F
authors: Sharopov
authors: J-P
authors: JP
authors: Girault
authors: M
authors: M
authors: Wink
authors: R
authors: R
authors: Lafont
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry; D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D019660:Malvaceae / Q000737:chemistry*; D015394:Molecular Structure; D045792:Norisoprenoids / Q000737:chemistry* / Q000302:isolation & purification; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002109:Caffeic Acids; D005960:Glucosides; D045792:Norisoprenoids; D010936:Plant Extracts; C438027:roseoside; D011794:Quercetin; C040048:caffeic acid
keywords: Abutilon theophrasti; NMR; anti-inflammatory; antioxidant; cytotoxicity; mass-spectroscopy; roseoside