title: Effect of laser treatment on the attachment and viability of mesenchymal stem cell responses on shape memory NiTi alloy.
abstract: The objectives of this study were to investigate the effect of laser-induced surface features on the morphology, attachment and viability of mesenchymal stem cells (MSCs) at different periods of time, and to evaluate the biocompatibility of different zones: laser-melted zone (MZ), heat-affected zone (HAZ) and base metal (BM) in laser-treated NiTi alloy. The surface morphology and composition were studied by scanning electron microscope (SEM) and X-ray photoemission spectroscopy (XPS), respectively. The cell morphology was examined by SEM while the cell counting and viability measurements were done by hemocytometer and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The results indicated that the laser-induced surface features, such as surface roughening, presence of anisotropic dendritic pattern and complete surface Ni oxidation were beneficial to improve the biocompatibility of NiTi as evidenced by the highest cell attachment (4 days of culture) and viability (7 days of culture) found in the MZ. The biocompatibility of the MZ was the best, followed by the BM with the HAZ being the worst. The defective and porous oxide layer as well as the coarse grained structure might attribute to the inferior cell attachment (4 days of culture) and viability (7 days of culture) on the HAZ compared with the BM which has similar surface morphology.
authors: School of Mechanical and Aerospace Engineering, Queen's University, Belfast, Northern Ireland, UK. Electronic address: c.w.chan@qub.ac.uk.
authors: C W
authors: CW
authors: Chan
authors: School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln, Lincolnshire LN6 7TU, UK.
authors: I
authors: I
authors: Hussain
authors: Laser Engineering and Manufacturing Research Group, Faculty of Science and Engineering, University of Chester, Parkgate Road, Chester, CH1 4BJ, UK.
authors: D G
authors: DG
authors: Waugh
authors: Laser Engineering and Manufacturing Research Group, Faculty of Science and Engineering, University of Chester, Parkgate Road, Chester, CH1 4BJ, UK.
authors: J
authors: J
authors: Lawrence
authors: Department of Industrial and Systems Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
authors: H C
authors: HC
authors: Man
mesh_terms: D000497:Alloys / Q000737:chemistry; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000295:instrumentation; D048430:Cell Shape / Q000502:physiology; D002470:Cell Survival / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D007834:Lasers; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D009532:Nickel / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D009532:Nickel; D014025:Titanium
keywords: Fiber laser; Laser treatment; Mesenchymal stem cell (MSC); NiTi; Shape memory alloy (SMA)