title: Production and purification of a novel xanthan lyase from a xanthan-degrading Microbacterium sp. strain XT11.
abstract: A xanthan lyase was produced and purified from the culture supernatant of an excellent xanthan-modifying strain Microbacterium sp. XT11. Xanthan lyase was induced by xanthan but was inhibited by its structural monomer glucose. Its production by strain XT11 is much higher than that by all other reported strains. The purified xanthan lyase has a molecular mass of 110 kDa and a specific activity of 28.2 U/mg that was much higher than that of both Paenibacillus and Bacillus lyases. It was specific on the pyruvated mannosyl residue in the intact xanthan molecule, but about 50% lyase activity remained when xanthan was partially depyruvated. Xanthan lyase was optimally active at pH 6.0-6.5 and 40°C and alkali-tolerant at a high pH value of 11.0. The metal ions including K(+), Ca(2+), Na(+), Mg(2+), Mn(2+), and Li(+) strongly stimulated xanthan lyase activity but ions Zn(2+) and Cu(2+) were its inhibitor. Xanthan lyase should be a novel enzyme different from the other xanthan lyases ever reported.
authors: School of Biological Engineering, Dalian Polytechnic University, Ganjingqu, Dalian 116034, China.
authors: Fan
authors: 0000-0002-0573-5263
authors: F
authors: Yang
authors: School of Biological Engineering, Dalian Polytechnic University, Ganjingqu, Dalian 116034, China.
authors: Lan
authors: L
authors: Yang
authors: School of Biological Engineering, Dalian Polytechnic University, Ganjingqu, Dalian 116034, China.
authors: Xiaoyu
authors: X
authors: Guo
authors: School of Biological Engineering, Dalian Polytechnic University, Ganjingqu, Dalian 116034, China.
authors: Xue
authors: X
authors: Wang
authors: School of Biological Engineering, Dalian Polytechnic University, Ganjingqu, Dalian 116034, China.
authors: Lili
authors: L
authors: Li
authors: School of Biological Engineering, Dalian Polytechnic University, Ganjingqu, Dalian 116034, China.
authors: Zhicheng
authors: Z
authors: Liu
authors: School of Biological Engineering, Dalian Polytechnic University, Ganjingqu, Dalian 116034, China.
authors: Wei
authors: W
authors: Wang
authors: School of Biological Engineering, Dalian Polytechnic University, Ganjingqu, Dalian 116034, China.
authors: Xianzhen
authors: X
authors: Li
mesh_terms: D019757:Carbon-Oxygen Lyases / Q000737:chemistry* / Q000378:metabolism; D008835:Micrococcaceae / Q000201:enzymology*; D011135:Polysaccharides, Bacterial / Q000378:metabolism
chemical_list: D011135:Polysaccharides, Bacterial; D019757:Carbon-Oxygen Lyases; C115444:xanthan lyase; C002563:xanthan gum