title: In vitro pre-vascularisation of tissue-engineered constructs A co-culture perspective.
abstract: In vitro pre-vascularization is one of the main vascularization strategies in the tissue engineering field. Culturing cells within a tissue-engineered construct (TEC) prior to implantation provides researchers with a greater degree of control over the fate of the cells. However, balancing the diverse range of different cell culture parameters in vitro is seldom easy and in most cases, especially in highly vascularized tissues, more than one cell type will reside within the cell culture system. Culturing multiple cell types in the same construct presents its own unique challenges and pitfalls. The following review examines endothelial-driven vascularization and evaluates the direct and indirect role other cell types have in vessel and capillary formation. The article then analyses the different parameters researchers can modulate in a co-culture system in order to design optimal tissue-engineered constructs to match desired clinical applications.
authors: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia.
authors: Jeremy
authors: J
authors: Baldwin
authors: Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
authors: Mélanie
authors: M
authors: Antille
authors: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia ; Leibniz Institute of Polymer Research Dresden (IPF) & Max Bergmann Center of Biomaterials Dresden (MBC), Hohe Str. 6, 01069, Dresden, Germany.
authors: Ulrich
authors: U
authors: Bonda
authors: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia.
authors: Elena M
authors: EM
authors: De-Juan-Pardo
authors: University of Queensland, UQ Centre for Clinical Research, Royal Brisbane & Women's Hospital Campus, Building 71/918, Herston, QLD 4029, Australian ; The University of Queensland, UQ Diamantina Institute, Translational Research Institute, Brisbane, QLD, Australia.
authors: Kiarash
authors: K
authors: Khosrotehrani
authors: Griffith Health Institute, Regenerative Medicine Centre, Gold Coast, QLD 4222, Australia.
authors: Saso
authors: S
authors: Ivanovski
authors: Griffith Health Institute, Regenerative Medicine Centre, Gold Coast, QLD 4222, Australia.
authors: Eugen Bogdan
authors: EB
authors: Petcu
authors: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia.
authors: Dietmar Werner
authors: DW
authors: Hutmacher
keywords: Co-culture; Matrices; Tissue engineering; Vascularisation