title: Genotoxicity of sulcotrione pesticide and photoproducts on Allium cepa root meristem.
abstract: Contamination by toxic agents in the environment has become matters of concern to agricultural countries. Sulcotrione, a triketone herbicide used to control dicotyledonous weeds in maize culture is rapidly photolyzed on plant foliage and generate two main photoproducts the xanthene-1,9-dione-3,4-dihydro-6-methylsulfonyl and 2-chloro-4-mesylbenzoic acid (CMBA). The aim of this study was to analyze the potential toxicity of the herbicide and the irradiated herbicide cocktail. Cytotoxicity and genotoxicity of non irradiated and irradiated sulcotrione were investigated in Allium cepa test. The sulcotrione irradiation was monitored under sunlight simulated conditions to reach 50% of phototransformation. Concentrations of sulcotrione in the range 5 × 10(-)(9)-5 × 10(-)(5)M were tested. Cytological analysis of root tips cells showed that both non irradiated and irradiated sulcotrione caused a dose-dependent decrease of mitotic index with higher cytotoxicity for the irradiated herbicide which can lead to 24.2% reduction of mitotic index compared to water control. Concomitantly, chromosomal aberrations were observed in A.cepa root meristems. Both non irradiated sulcotrione and irradiated sulcotrione induced a dose-dependent increase of chromosomal abnormalities frequencies to a maximal value of 33.7%. A saturating effect in anomaly frequencies was observed in meristems treated with high concentrations of non irradiated sulcotrione only. These data suggest that photolyzed sulcotrione cocktail have a greater cytotoxicity and genotoxicity than parent molecule and question about the impact of photochemical process on environment.
authors: Clermont Université, UMR 547-UBP/INRA PIAF, Université Blaise Pascal, Campus Universitaire des Cézeaux, 24, Avenue des Landais, 63177 Aubière cedex, France.
authors: Eric
authors: E
authors: Goujon
authors: Clermont Université, UMR 547-UBP/INRA PIAF, Université Blaise Pascal, Campus Universitaire des Cézeaux, 24, Avenue des Landais, 63177 Aubière cedex, France.
authors: Chaima
authors: C
authors: Sta
authors: Clermont Université, CNRS, UMR 6296, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand (ICCF), 24, Avenue des Landais, 63177 Aubière cedex, France.
authors: Aurélien
authors: A
authors: Trivella
authors: Clermont Université, UMR 547-UBP/INRA PIAF, Université Blaise Pascal, Campus Universitaire des Cézeaux, 24, Avenue des Landais, 63177 Aubière cedex, France.
authors: Pascale
authors: P
authors: Goupil
authors: Clermont Université, CNRS, UMR 6296, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand (ICCF), 24, Avenue des Landais, 63177 Aubière cedex, France.
authors: Claire
authors: C
authors: Richard
authors: Clermont Université, UMR 547-UBP/INRA PIAF, Université Blaise Pascal, Campus Universitaire des Cézeaux, 24, Avenue des Landais, 63177 Aubière cedex, France. Electronic address: gerard.ledoigt@univ-bpclermont.fr.
authors: Gérard
authors: G
authors: Ledoigt
mesh_terms: D002869:Chromosome Aberrations / Q000139:chemically induced; D003512:Cyclohexanones / Q000633:toxicity*; D018519:Meristem / Q000187:drug effects* / Q000235:genetics; D008698:Mesylates / Q000633:toxicity*; D019697:Onions / Q000187:drug effects* / Q000235:genetics*; D010575:Pesticides / Q000633:toxicity*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics
chemical_list: D003512:Cyclohexanones; D008698:Mesylates; D010575:Pesticides; C079906:sulcotrione
keywords: Allium cepa; Genotoxicity; Pesticide; Photolysis; Photoproducts; Sulcotrione