title: Lethal Encephalitozoon cuniculi genotype III infection in Steppe lemmings (Lagurus lagurus).
abstract: Microsporidia are ubiquitous, spore-forming, intracellular parasites infecting invertebrates and vertebrates. Some of them are important opportunistic pathogens in humans, including three species of genus Encephalitozoon. Intraspecies genetic variation with a different range of hosts is known in Encephalitozoon cuniculi distinguishing four genotypes. Recently, E. cuniculi is often observed in pet animals, mainly E. cuniculi genotype I in pet rabbits. This study described a fatal encephalitozoonosis in a group of pet rodents Steppe lemmings (Lagurus lagurus). The animals were presented with progressive weight loss, aggression, cannibalism, purulent conjunctivitis and hind limb paresis. Death occurred within 48 h after the onset of clinical signs. The group comprised of 15 animals was affected and died within a period of three months. Post-mortal examination did not show any macroscopic changes. Microsporidial vacuoles with typical spores were found in brain and kidney tissues and E. cuniculi DNA in all tested organs. The internal transcribed spacer region (ITS) of rRNA gene showed 100% homology with E. cuniculi genotype III previously identified in dogs, tamarin colonies from zoos, swine, birds and humans. Pet lemmings could represent a new potential source of the infection for their breeders.
authors: Department of Pathological Morphology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic; Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic.
authors: Lada
authors: L
authors: Hofmannová
authors: Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic.
authors: Bohumil
authors: B
authors: Sak
authors: Avian & Exotic Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic.
authors: Vladimír
authors: V
authors: Jekl
authors: Avian & Exotic Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic.
authors: Andrea
authors: A
authors: Mináriková
authors: Department of Pathological Morphology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic.
authors: Miša
authors: M
authors: Skorič
authors: Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Branišovská 31, 370 05 České Budějovice, Czech Republic; Faculty of Agriculture, University of South Bohemia in České Budějovice, Branišovská 31, 370 05 České Budějovice, Czech Republic. Electronic address: kvac@paru.cas.cz.
authors: Martin
authors: M
authors: Kváč
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000469:parasitology*; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D009670:Encephalitozoon cuniculi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016890:Encephalitozoonosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D005260:Female; D005838:Genotype; D008297:Male; D057805:Pets; D011817:Rabbits; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Encephalitozoon cuniculi genotype III; Histology; Lethal infection; PCR; Steppe lemmings