title: Coexpressed auxiliary subunits exhibit distinct modulatory profiles on AMPA receptor function.
abstract: Gating properties and surface trafficking of AMPA receptors (AMPARs) are modulated by auxiliary subunits. Here we studied the function of coexpressed auxiliary subunits belonging to two different classes. We focused on TARP γ-8 and CKAMP44 in dentate gyrus (DG) granule cells, since both subunits are highly expressed in this cell type. TARP γ-8 and CKAMP44 decrease the rate of deactivation but have an opposing influence on receptor desensitization, which accounts for their differential modulation of synaptic short-term plasticity. Furthermore, long-term plasticity (LTP) requires TARP γ-8 but not CKAMP44. The coexpression of both auxiliary subunits is necessary for the efficient targeting of AMPARs to the cell surface of DG granule cells. Finally, electrophysiological and biochemical evidence support the notion that CKAMP44 and TARP γ-8 can be contained in the same AMPAR complex.
authors: Department of Clinical Neurobiology, University Hospital and DKFZ Heidelberg, 69120 Heidelberg, Germany.
authors: Konstantin
authors: K
authors: Khodosevich
authors: Synaptic Signalling and Neurodegeneration, German Center for Neurodegenerative Diseases (DZNE), 53175 Bonn, Germany; Synaptic Signalling and Neurodegeneration, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
authors: Eric
authors: E
authors: Jacobi
authors: Synaptic Signalling and Neurodegeneration, German Center for Neurodegenerative Diseases (DZNE), 53175 Bonn, Germany; Synaptic Signalling and Neurodegeneration, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
authors: Paul
authors: P
authors: Farrow
authors: Department of Clinical Neurobiology, University Hospital and DKFZ Heidelberg, 69120 Heidelberg, Germany.
authors: Anton
authors: A
authors: Schulmann
authors: Department of Clinical Neurobiology, University Hospital and DKFZ Heidelberg, 69120 Heidelberg, Germany.
authors: Alexandru
authors: A
authors: Rusu
authors: Max Planck Institute for Medical Research, 69120 Heidelberg, Germany.
authors: Ling
authors: L
authors: Zhang
authors: Max Planck Institute for Medical Research, 69120 Heidelberg, Germany.
authors: Rolf
authors: R
authors: Sprengel
authors: Department of Clinical Neurobiology, University Hospital and DKFZ Heidelberg, 69120 Heidelberg, Germany.
authors: Hannah
authors: H
authors: Monyer
authors: Synaptic Signalling and Neurodegeneration, German Center for Neurodegenerative Diseases (DZNE), 53175 Bonn, Germany; Synaptic Signalling and Neurodegeneration, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany. Electronic address: engelhardt@dzne.de.
authors: Jakob
authors: J
authors: von Engelhardt
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D018891:Dentate Gyrus / Q000378:metabolism; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D055786:Gene Knockout Techniques; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: C548320:CKAMP44 protein, mouse; D015220:Calcium Channels; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D018091:Receptors, AMPA; C515550:TARP gamma-8 protein, mouse