title: Effect of oxidatively modified and non-modified human serum albumin on luminol-dependent chemiluminescence of human peripheral blood leukocytes stimulated with opsonized zymosan.
abstract: We studied the effects of native and oxidized human serum albumin on luminol-dependent chemiluminescence of human peripheral blood leukocytes stimulated with opsonized zymosan. Human serum albumin was added simultaneously with opsonized zymosan at the beginning of the chemiluminescent reaction. Otherwise, leukocytes were incubated with human serum albumin at 37°C for various periods before addition of opsonized zymosan. Oxidized human serum albumin was obtained by the method of metal-catalyzed oxidation. In control to non-modified albumin, oxidized albumin produced an inhibitory effect on luminol-dependent chemiluminescence of leukocytes. These changes were observed in experiments with addition of oxidized albumin at the beginning of a chemiluminescent reaction and after incubation of study agent with cells.
authors: Laboratory for Biophysical Bases of Pathology, Research Institute of Physicochemical Medicine, Federal Medical-Biological Agency, Moscow, Russia.
authors: A P
authors: AP
authors: Piryazev
authors: A P
authors: AP
authors: Azizova
authors: A V
authors: AV
authors: Aseichev
authors: V I
authors: VI
authors: Sergienko
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D009895:Opsonin Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000276:immunology; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism*; D015054:Zymosan / Q000378:metabolism
chemical_list: D049408:Luminescent Agents; D009895:Opsonin Proteins; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D008165:Luminol; D015054:Zymosan