title: Effects of dietary sea cucumber saponin on the gene expression rhythm involved in circadian clock and lipid metabolism in mice during nighttime-feeding.
abstract: In mammals, clock rhythms exist not only in the suprachiasmatic nucleus, which is entrained by light/dark (LD) cycles, but also in most peripheral tissues. Recent studies have revealed that most physiology and behavior are subject to well-controlled daily oscillations; similarly, metabolic state influences the diurnal rhythm too. Previous studies have indicated that dietary sea cucumber saponin (SCS) could improve glucose and lipid metabolism of rodent. However, whether SCS could affect the expression of clock genes, which is involved in lipid metabolism, is unknown at present. The aim of this study is to investigate the effects of SCS on the clock and clock-controlled genes involved in lipid metabolism. ICR male mice were divided into a control and SCS group mice (add 0.03% sea cucumber saponin to regular chow) and were fed at night (2030-0830 hours). After 2 weeks, clock genes expression in brain and liver, blood glucose, hormones, and lipid metabolic markers were analyzed. The results showed that dietary SCS caused alteration in rhythms and/or amplitudes of clock genes was more significant in brain than in liver. In addition, peroxisome proliferator-activated receptor (PPARα), sterol regulatory element binding protein-1c (SREBP-1c), together with their target genes carnitine palmitoyl transferase (CPT), and fatty acid synthase (FAS) showed marked changes in rhythm and/or amplitude in SCS group mice. These results suggested that SCS could affect the daily expression patterns of clock genes in brain and liver tissues, and alter the clock-controlled genes involved in lipid metabolism.
authors: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, Shandong Province, China.
authors: Min
authors: M
authors: Wen
authors: Jie
authors: J
authors: Cui
authors: Jie
authors: J
authors: Xu
authors: Yong
authors: Y
authors: Xue
authors: Jingfeng
authors: J
authors: Wang
authors: Changhu
authors: C
authors: Xue
authors: Yuming
authors: Y
authors: Wang
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics; D000273:Adipose Tissue / Q000033:anatomy & histology / Q000187:drug effects; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D056926:CLOCK Proteins / Q000235:genetics; D057906:Circadian Clocks / Q000187:drug effects* / Q000235:genetics*; D056931:Cryptochromes / Q000235:genetics; D019587:Dietary Supplements; D005247:Feeding Behavior; D015870:Gene Expression / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects; D056950:Period Circadian Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012503:Saponins / Q000008:administration & dosage*; D012616:Sea Cucumbers / Q000737:chemistry*
chemical_list: D056930:ARNTL Transcription Factors; C538961:Bmal1 protein, mouse; D015415:Biomarkers; C538964:Cry1 protein, mouse; C538967:Cry2 protein, mouse; D056931:Cryptochromes; C539023:Per1 protein, mouse; C512645:Per3 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D012503:Saponins; D056926:CLOCK Proteins; C538944:Clock protein, mouse