title: Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional rickets.
abstract: Nutritional rickets is a worldwide public health problem; however, the current diagnostic methods retain shortcomings for accurate diagnosis of nutritional rickets. To identify urinary biomarkers associated with nutritional rickets and establish a noninvasive diagnosis method, urinary metabonomics analysis by ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry and multivariate statistical analysis were employed to investigate the metabolic alterations associated with nutritional rickets in 200 children with or without nutritional rickets. The pathophysiological changes and pathogenesis of nutritional rickets were illustrated by the identified biomarkers. By urinary metabolic profiling, 31 biomarkers of nutritional rickets were identified and five candidate biomarkers for clinical diagnosis were screened and identified by quantitative analysis and receiver operating curve analysis. Urinary levels of five candidate biomarkers were measured using mass spectrometry or commercial kits. In the validation step, the combination of phosphate and sebacic acid was able to give a noninvasive and accurate diagnostic with high sensitivity (94.0%) and specificity (71.2%). Furthermore, on the basis of the pathway analysis of biomarkers, our urinary metabonomics analysis gives new insight into the pathogenesis and pathophysiology of nutritional rickets.
authors: National Key Disciplines of Nutrition and Food Hygiene, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University , 157 Baojian Road, Nangang District, Harbin 150081, P. R. China.
authors: Maoqing
authors: M
authors: Wang
authors: Xue
authors: X
authors: Yang
authors: Lihong
authors: L
authors: Ren
authors: Songtao
authors: S
authors: Li
authors: Xuan
authors: X
authors: He
authors: Xiaoyan
authors: X
authors: Wu
authors: Tingting
authors: T
authors: Liu
authors: Liqun
authors: L
authors: Lin
authors: Ying
authors: Y
authors: Li
authors: Changhao
authors: C
authors: Sun
mesh_terms: D015415:Biomarkers / Q000737:chemistry / Q000652:urine*; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D007223:Infant; D016018:Least-Squares Analysis; D008297:Male; D055442:Metabolome / Q000502:physiology*; D055432:Metabolomics / Q000379:methods*; D012372:ROC Curve; D012279:Rickets / Q000175:diagnosis* / Q000378:metabolism / Q000652:urine; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers