title: Molecular species analysis of monosialogangliosides from sea urchin Strongylocentrotus nudus by RPLC-ESI-MS/MS.
abstract: Sea urchin gangliosides have been proved to contain neuritogenic activities, which related to their molecular compositions. This study reports a method utilizing reversed-phase chromatography coupled to mass spectrometry for structure investigation and molecular species determination of the monosialogangliosides from sea urchin Strongylocentrotus nudus. Two types of sulfated and nonsulfated monosialogangliosides were isolated from the sea urchin ovary. In MS(2) spectra of both nonsulfated monosialoganglioside and sulfated monosialoganglioside, 2-6 linked sialic acids were identified by the characteristic fragments of (0,4)A₂-CO₂ and (0,2)A₁. Fragment ions at m/z 139.1 and m/z 169.1 of nonsulfated monosialoganglioside might be characteristic for 8-sulfated sialic acid residue. Retention time of the molecules was effectively used in the characterization of unknown molecules, and molecules that differ in mass by only 0.04 Da were easily differentiated.
authors: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, PR China. Electronic address: cpx69@139.com.
authors: Pei-Xu
authors: PX
authors: Cong
authors: School of Food Biological and Engineering, JiangSu University, Zhenjiang, Jiangsu Province 212013, PR China. Electronic address: Xiyuan2008@ujs.edu.cn.
authors: Rui-Chang
authors: RC
authors: Gao
authors: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, PR China. Electronic address: xuech@ouc.edu.cn.
authors: Chang-Hu
authors: CH
authors: Xue
authors: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, PR China. Electronic address: lizhaojie@ouc.edu.cn.
authors: Zhao-Jie
authors: ZJ
authors: Li
authors: TechnicalCenter of Entry-Exit Inspection and Quarantine, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao, Shandong Province 266002, PR China. Electronic address: light04@126.com.
authors: Hong-Wei
authors: HW
authors: Zhang
authors: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, PR China. Electronic address: micro_pcsir@yahoo.com.
authors: Muhammad Naseem
authors: MN
authors: Khan
authors: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, PR China. Electronic address: xueyong@ouc.edu.cn.
authors: Yong
authors: Y
authors: Xue
authors: Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: sugawara@kais.kyoto-u.ac.jp.
authors: Tatsuya
authors: T
authors: Sugawara
authors: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, PR China. Electronic address: xujie9@ouc.edu.cn.
authors: Jie
authors: J
authors: Xu
mesh_terms: D000818:Animals; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005260:Female; D005732:Gangliosides / Q000032:analysis*; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D012617:Sea Urchins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D005732:Gangliosides; C025447:sialogangliosides; D019158:N-Acetylneuraminic Acid
keywords: Ceramide moiety; Fragmentation; Ganglioside; Molecular species; PRLC-MS/MS; Sialic acid