title: Molecular cloning of peroxinectin gene and its expression in response to peptidoglycan and Vibrio harveyi in Indian white shrimp Fenneropenaeus indicus.
abstract: The cDNA sequence of peroxinectin was obtained from the haemocytes of Indian white shrimp Fenneropenaeus indicus using RT-PCR and RACE. Fenneropenaeus indicus peroxinectin (Fi-Pxn) sequence has an open reading frame (ORF) of 2415 bp encoding a protein of 804 amino acids with 21 residues signal sequence. The mature protein has molecular mass of 89.8 kDa with an estimated pI of 8.6. Two putative integrin-binding motifs, RGD and KGD, were observed at the basic N-terminal and C-terminal part of the mature aminoacid sequence. Fi-Pxn nucleotide sequence comparison showed high homology to mud crab Scylla serrata (89%) and to various vertebrate and invertebrate species. qRT-PCR showed peroxinectin mRNA transcript in haemocytes of F. indicus increased at 6 h post injection of peptidoglycan and Vibrio harveyi. The Fi-Pxn was mainly expressed in the tissues of haemocytes and the heart. The moulting stage responses showed Fi-Pxn expression in premoult stages D0/1 and D0/2.
authors: Crustacean Molecular Biology & Genomics Lab, Department of Animal Health and Management, Alagappa University , Karaikudi, Tamil Nadu , India.
authors: Sathappan
authors: S
authors: Shanthi
authors: Sivalingam
authors: S
authors: Manju
authors: Perumal
authors: P
authors: Rajakumaran
authors: Baskaralingam
authors: B
authors: Vaseeharan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006434:Hemocytes / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics* / Q000382:microbiology*; D010457:Peptidoglycan / Q000494:pharmacology*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014733:Vibrio / Q000502:physiology*
chemical_list: D015815:Cell Adhesion Molecules; D010457:Peptidoglycan; D012333:RNA, Messenger
keywords: F. indicus; adhesion; cell; haemolymph; peroxinectin