title: Efficient determination of protocatechuic acid in fruit juices by selective and rapid magnetic molecular imprinted solid phase extraction coupled with HPLC.
abstract: Magnetic molecular imprinted polymers (MMIPs) have been prepared as solid phase material to selectively extract protocatechuic acid (PCA) from fruit juices with high capacity and fast binding kinetics. The resulting MMIPs were characterized by TEM, FT-IR, TGA, and VSM. The adsorption process between PCA and MMIPs followed Langumuir adsorption isotherm with maximum adsorption capacity at 7.5 mg/g and pseudo-second-order reaction kinetics with fast binding kinetics (equilibrium time at 40 min). In addition, the prepared MMIPs showed rapid magnetic separation (10 s) and reusability (retained 94.9% after six cycles). Subsequently, MMIPs were successfully applied for selective enrichment and determination of PCA from fruit juices (0.45 μg/mL in grape juice but not detected in apple juice, pineapple juice, orange juice, and peach juice) with satisfactory recoveries (92-107%). The results indicated that synthesized MMIPs can be used for efficient and selective extraction of PCA from complex matrices.
authors: College of Sciences, Central South University of Forestry and Technology , Changsha 410004, People's Republic of China.
authors: Lianwu
authors: L
authors: Xie
authors: Junfang
authors: J
authors: Guo
authors: Yuping
authors: Y
authors: Zhang
authors: Shuyun
authors: S
authors: Shi
mesh_terms: D000327:Adsorption; D000975:Antioxidants / Q000032:analysis* / Q000302:isolation & purification; D001628:Beverages / Q000032:analysis* / Q000191:economics; D002681:China; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements; D005290:Ferric Compounds / Q000737:chemistry; D005525:Food-Processing Industry / Q000379:methods*; D005638:Fruit / Q000737:chemistry* / Q000191:economics; D062385:Hydroxybenzoates / Q000032:analysis* / Q000302:isolation & purification; D007700:Kinetics; D060328:Magnetic Phenomena; D008863:Microspheres; D054802:Molecular Imprinting; D010316:Particle Size; D011145:Polyvinyls / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction; D013056:Spectrophotometry, Ultraviolet; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005290:Ferric Compounds; D062385:Hydroxybenzoates; D011145:Polyvinyls; C000499:ferric oxide; C009091:protocatechuic acid; D012822:Silicon Dioxide