title: Evaluation of Single Nucleotide Polymorphisms (SNPs) Genotyped by the Illumina Bovine SNP50K in Cattle Focusing on Hanwoo Breed.
abstract: In the present study, we evaluated the informativeness of SNPs genotyped by the Illumina Bovine SNP50K assay in different cattle breeds. To investigate these on a genome-wide scale, we considered 52,678 SNPs spanning the whole autosomal and X chromosomes in cattle. Our study samples consists of six different cattle breeds. Across the breeds approximately 72 and 6% SNPs were found polymorphic and fixed or close to fix in all the breeds, respectively. The variations in the average minor allele frequency (MAF) were significantly different between the breeds studied. The level of average MAF observed in Hanwoo was significantly lower than the other breeds. Hanwoo breed also displayed the lowest number of polymorphic SNPs across all the chromosomes. More importantly, this study indicated that the Bovine SNP50K assay will have reduced power for genome-wide association studies in Hanwoo as compared to other cattle breeds. Overall, the Bovine SNP50K assay described in this study offer a useful genotyping platform for mapping quantitative trait loci (QTLs) in the cattle breeds. The assay data represent a vast and generally untapped resource to assist the investigation of the complex production traits and the development of marker-assisted selection programs.
authors: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea .
authors: Hailu
authors: H
authors: Dadi
authors: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea .
authors: Jong-Joo
authors: JJ
authors: Kim
authors: Department of Animal Science, Kyungbook National University, Sangju, Kyungbook 742-711, Korea .
authors: Duhak
authors: D
authors: Yoon
authors: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea .
authors: Kwan-Suk
authors: KS
authors: Kim
keywords: Fixed SNPs; Hanwoo Breed; Minor Allele Frequency; Polymorphic SNPs