title: Speed control for neuronal migration in the postnatal brain by Gmip-mediated local inactivation of RhoA.
abstract: Throughout life, new neurons generated in the ventricular-subventricular zone take the long journey to the olfactory bulb. The intracellular mechanisms that precisely control the neurons' migration speed, enabling their well-organized movement, remain unclear. Rho signalling is known to affect the morphology and movement of various cell types, including neurons. Here we identify Gem-interacting protein (Gmip), a RhoA-specific GTPase-activating protein, as a key factor in saltatory neuronal migration. RhoA is activated at the proximal leading process of migrating neurons, where Gmip is also localized and negatively regulates RhoA. Gmip controls the saltatory movement of neurons that regulate their migration speed and 'stop' positions in the olfactory bulb, thereby altering the neural circuitry. This study demonstrates that Gmip serves as a brake for the RhoA-mediated movement of neuronal somata, and highlights the significance of speed control in the well-organized neuronal migration and the maintenance of neuronal circuits in the postnatal brain.
authors: 1] Department of Developmental and Regenerative Biology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan [2] Department of Anesthesiology and Medical Crisis Management, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan [3].
authors: Haruko
authors: H
authors: Ota
authors: 1] Department of Developmental and Regenerative Biology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan [2].
authors: Takao
authors: T
authors: Hikita
authors: 1] Department of Developmental and Regenerative Biology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan [2].
authors: Masato
authors: M
authors: Sawada
authors: Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.
authors: Tomoki
authors: T
authors: Nishioka
authors: Department of Developmental and Regenerative Biology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.
authors: Mami
authors: M
authors: Matsumoto
authors: Department of Developmental and Regenerative Biology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.
authors: Masayuki
authors: M
authors: Komura
authors: Department of Developmental and Regenerative Biology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.
authors: Akihisa
authors: A
authors: Ohno
authors: Department of Developmental and Regenerative Biology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.
authors: Yukiyo
authors: Y
authors: Kamiya
authors: Department of Developmental and Regenerative Biology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.
authors: Takuya
authors: T
authors: Miyamoto
authors: Department of Pathology, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.
authors: Naoya
authors: N
authors: Asai
authors: Department of Pathology, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.
authors: Atsushi
authors: A
authors: Enomoto
authors: Department of Pathology, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.
authors: Masahide
authors: M
authors: Takahashi
authors: Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.
authors: Kozo
authors: K
authors: Kaibuchi
authors: Department of Anesthesiology and Medical Crisis Management, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.
authors: Kazuya
authors: K
authors: Sobue
authors: Department of Developmental and Regenerative Biology, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.
authors: Kazunobu
authors: K
authors: Sawamoto
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000166:cytology* / Q000254:growth & development*; D001973:Bromodeoxyuridine; D002465:Cell Movement / Q000502:physiology*; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D009474:Neurons / Q000502:physiology*; D034622:RNA Interference; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*; D018709:Statistics, Nonparametric; D059008:Time-Lapse Imaging; D033921:Vesicular Transport Proteins / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: D001973:Bromodeoxyuridine; D020690:GTPase-Activating Proteins; D008840:Microfilament Proteins; D033921:Vesicular Transport Proteins; D020742:rhoA GTP-Binding Protein; C000602556:Gmip protein, mouse; C527550:Ccdc88a protein, mouse