title: Inhibitory potential of Turbinaria ornata against key metabolic enzymes linked to diabetes.
abstract: One of the therapeutic approaches in treating diabetes is to reduce postprandial hyperglycemia by inhibiting major carbohydrate hydrolyzing enzymes. In the present study, crude extracts of marine seaweed, Turbinaria ornata, were tested for their antidiabetic potential using enzyme inhibitory assays (α-amylase, α-glucosidase, and dipeptidyl peptidase-IV). Among the tested extracts, methanol and acetone extracts showed significant inhibitory effects on α-amylase (IC50 250.9 μg/mL), α-glucosidase (535.6 μg/mL), and dipeptidyl peptidase-4 (55.2 μg/mL), respectively. Free radical scavenging activity of these extracts was analyzed using DPPH assay (65%). Extracts were tested for in vitro toxicity using DNA fragmentation assay, haemolytic assay, and MTT assay. None of the extracts showed toxicity in tested models. Furthermore, GC-MS analysis of lead extracts showed the presence of major compounds, hentriacontane, z, z-6, 28-heptatriactontadien-2-one, 8-heptadecene, and 1-heptacosanol. Our findings suggest that Turbinaria ornata can be used as a potential source for further in vivo studies in controlling hyperglycemia.
authors: Marine Biotechnology and Biomedicine Laboratory, School of Biosciences and Technology, VIT University, Vellore 632007, India.
authors: P S
authors: PS
authors: Unnikrishnan
authors: Marine Biotechnology and Biomedicine Laboratory, School of Biosciences and Technology, VIT University, Vellore 632007, India.
authors: K
authors: K
authors: Suthindhiran
authors: Marine Biotechnology and Biomedicine Laboratory, School of Biosciences and Technology, VIT University, Vellore 632007, India.
authors: M A
authors: MA
authors: Jayasri
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D003920:Diabetes Mellitus / Q000201:enzymology*; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000187:drug effects*; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D012624:Seaweed / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D009842:Oligopeptides; D010851:Picrates; D010936:Plant Extracts; C041445:diprotin A; C004931:1,1-diphenyl-2-picrylhydrazyl; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D018819:Dipeptidyl Peptidase 4