title: Multidrug-resistant Acinetobacter baumannii strains carrying the bla(OxA-23) and the bla(GES-11) genes in a neonatology center in Tunisia.
abstract: Multidrug-resistant and difficult-to-treat Acinetobacter baumannii may be responsible for nosocomial infections. The production of carbapenem-hydrolyzing class D β-lactamases (CHDLs) and extended-spectrum β-lactamase (ESBLs) of the GES type possessing a carbapenemase activity has been increasingly reported worldwide in A. baumannii. The aim of this study was to analyze the resistance mechanisms of two carbapenem resistant A. baumannii clinical isolates recovered in a neonatology center in the center-east of Tunisia. Two carbapenem resistant A. baumannii isolates were recovered. The first isolate co-harbored the blaGES-11 ESBL gene and the blaOxA-23 CHDL gene. Analyses of the genetic location indicated that the blaGES-11 gene was plasmid located (Gr6). However, the blaOxA-23 gene was located on the chromosome. The second strain had only the blaOxA-23 CHDL gene, which was plasmid located. This study showed the first description of the GES-type β-lactamase in A. baumannii in Tunisia.
authors: INSERM U914, South-Paris Medical School, K.-Bicêtre, France; Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie, Monastir, Tunisia.
authors: Karama
authors: K
authors: Charfi-Kessis
authors: Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie, Monastir, Tunisia. Electronic address: wejdene.mansour@gmail.com.
authors: Wejdene
authors: W
authors: Mansour
authors: Laboratoire de Microbiologie, CHU Taher Sfar Mahdia, Tunisia.
authors: Anis
authors: A
authors: Ben Haj Khalifa
authors: Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie, Monastir, Tunisia.
authors: Maha
authors: M
authors: Mastouri
authors: INSERM U914, South-Paris Medical School, K.-Bicêtre, France; Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland.
authors: Patrice
authors: P
authors: Nordmann
authors: Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives LR99ES27, Faculté de Pharmacie, Monastir, Tunisia.
authors: Mahjoub
authors: M
authors: Aouni
authors: INSERM U914, South-Paris Medical School, K.-Bicêtre, France; Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland.
authors: Laurent
authors: L
authors: Poirel
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D002876:Chromosomes, Bacterial; D003428:Cross Infection / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D005798:Genes, Bacterial; D006761:Hospitals; D010957:Plasmids; D014416:Tunisia; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases
keywords: Acinetobacter baumannii; Carbapenem resistance; GES-11; Neonatology center; OXA-23; Tunisia