title: Surface proteins of Staphylococcus aureus play an important role in experimental skin infection.
abstract: Staphylococcus aureus is the most common cause of skin infections that range from mild diseases up to life-threatening conditions. Mechanisms of S. aureus virulence in those infections remain poorly studied. To investigate the impact of S. aureus surface proteins on skin infection, we used mouse models of skin abscess formation and skin necrosis, induced by a subcutaneous injection of bacteria. In the skin abscess model, a sortase-deficient S. aureus strain lacking all of its cell-wall anchored proteins was less virulent than its wild-type strain. Also, strains specifically lacking protein A, fibronecting binding proteins, clumping factor A or surface protein SasF were impaired in their virulence. When a model of dermonecrosis was studied, the S. aureus surface proteins could not be shown to be involved. In summary, surface proteins play an important role in virulence of S. aureus skin abscess infections, but not in formation of skin necrosis.
authors: Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
authors: Jakub
authors: J
authors: Kwiecinski
authors: Tao
authors: T
authors: Jin
authors: Elisabet
authors: E
authors: Josefsson
mesh_terms: D000818:Animals; D002473:Cell Wall / Q000378:metabolism / Q000382:microbiology; D003030:Coagulase / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D012871:Skin Diseases / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000473:pathology*; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000472:pathogenicity*; D037521:Virulence Factors / Q000378:metabolism
chemical_list: C515944:ClfA protein, Staphylococcus aureus; D003030:Coagulase; D008565:Membrane Proteins; D013205:Staphylococcal Protein A; D037521:Virulence Factors
keywords: SSTI; Staphylococcus aureus; abscess; necrosis; skin; surface proteins; virulence