title: Hydroalcoholic extract of Crocus sativus effects on bronchial inflammatory cells in ovalbumin sensitized rats.
abstract: OBJECTIVE
  Inflammation is one of the major components of asthma. Our aim was to evaluate the effects of C. sativus extract on total and differential white blood cells (WBC) count in lung lavage fluid (LLF) of ovalbumin-sensitized rats.
  
  
  METHODS
  Forty rats were divided into five groups (n = 8 for each group) as control (C), sensitized with injection and inhalation of ovalbumin (OA) alone (S), and three groups of sensitized and treated with different doses of C. sativus extract (S50EX, S100EX, and S200EX groups). Total and differential WBC counts of LLF were evaluated in control, sensitized, and treated sensitized groups.
  
  
  RESULTS
  Total WBC count, neutrophil, and eosinophil percentage in LLF were increased in sensitized animals compared with the control group (p0.001). Treatment of sensitized animals with all doses of the extract significantly reduced WBC number and the percentage of neutrophil and eosinophil compared with the sensitized animals (p0.01-0.001).
  
  
  CONCLUSIONS
  According to these results, the extract of C. sativus could be effective on alleviating lung inflammatory cells specially eosinophils in lung lavage of sensitized animals which may indicate a preventive effect of this plant on lung inflammation in asthma.
authors: Applied Physiology Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad , I. R. Iran.
authors: Maryam
authors: M
authors: Mahmoudabady
authors: Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, I. R. Iran.
authors: Ali
authors: A
authors: Neamati
authors: Department of Biology, Payame Noor University, Boshruyeh, South Khorasan, I. R. Iran.
authors: Somayyeh
authors: S
authors: Vosooghi
authors: Department of Biology, Faculty of Science, Arsanjan Branch, Islamic Azad University, I. R. Iran.
authors: Heydar
authors: H
authors: Aghababa
keywords: Asthma; Crocus sativus; Inﬂammation; Ovalbumin; Rat; Sensitization; WBC