title: Dynamic recruitment of protein tyrosine phosphatase PTPD1 to EGF stimulation sites potentiates EGFR activation.
abstract: Balanced activity of protein tyrosine kinases and phosphatases (PTPs) controls tyrosine phosphorylation levels and, consequently, is needed to prevent pathologies like cancer. Phosphatase activity is tightly regulated in space and time. Thus, in order to understand how phospho-tyrosine signalling is regulated, the intracellular dynamics of PTPs should be investigated. Here, we have studied the intracellular dynamics of PTPD1, a FERM (four-point-one, ezrin, radixin, moesin) domain-containing PTP that is over expressed in cancer cells and potentiates EGFR signalling. Whereas PTPD1 was excluded from E-cadherin rich cell-cell adhesions in epithelial cell monolayers, it diffused from the cytoplasm to those membranes in contact with the extracellular medium. Localisation of PTPD1 at the plasma membrane was mediated by its FERM domain and enabled the formation of EGFR/PTPD1-containing signalling complexes that pre-existed at the plasma membrane before EGF stimulation. PTPD1 and EGFR transiently co-localised at EGF stimulation sites until the formation of macropinosomes containing active species of EGFR. Interference of PTPD1 expression caused a decrease in EGFR phosphorylated species at the periphery of the cell. Presented data suggest that the transient formation of dynamic PTPD1/EGFR signalling complexes strengthens EGF signalling by promoting the spatial propagation of EGFR phosphorylated species.
authors: Department of Immunology, School of Medicine, Complutense University and '12 de Octubre' Health Research Institute, Madrid, Spain.
authors: Pedro
authors: P
authors: Roda-Navarro
authors: Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.
authors: Philippe I
authors: PI
authors: Bastiaens
mesh_terms: D002448:Cell Adhesion / Q000235:genetics; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D061986:MCF-7 Cells; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D010766:Phosphorylation; D017434:Protein Structure, Tertiary / Q000235:genetics; D054558:Protein Tyrosine Phosphatases, Non-Receptor / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D004815:Epidermal Growth Factor; C512478:EGFR protein, human; D066246:ErbB Receptors; C409985:PTPN21 protein, human; D054558:Protein Tyrosine Phosphatases, Non-Receptor