title: Host range of the potential biopesticide Pea Albumin 1b (PA1b) is limited to insects.
abstract: The Pea Albumin 1 subunit b (PA1b) peptide is an entomotoxin extracted from legume seeds with lethal activity towards several insect pests. Its toxic activity occurs after the perception of PA1b by a plasmalemmic proton pump (V-ATPase) in the insects. Assays revealed that PA1b showed no activity towards mammalian cells displaying high V-ATPase activity. Similarly, PA1b displayed no binding activity and no biological activity towards other non-insect organisms. We demonstrate here that binding to labelled PA1b was found in all the insect families tested, regardless of the sensitivity or insensitivity of the individual species. The coleopteran Bruchidae, which are mainly legume seed pests, were found to be fully resistant. A number of insect species were seen to be insensitive to the toxin although they exhibited binding activity for the labelled PA1b. The fruit fly, Drosophila melanogaster (Diptera), was generally insensitive when maintained on an agar diet, but the fly appeared to be sensitive to PA1b in bioassays using a different diet. In conclusion, the PA1b toxin provides legumes with a major source of resistance to insects, and insects feeding on legume seeds need to overcome this plant resistance by disrupting the PA1b - V-ATPase interaction.
authors: INRA, INSA-Lyon, Université de Lyon, UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, Bat. Louis-Pasteur, 20 av. Albert Einstein, F-69621 Villeurbanne, France.
authors: Isabelle
authors: I
authors: Rahioui
authors: INRA, INSA-Lyon, Université de Lyon, UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, Bat. Louis-Pasteur, 20 av. Albert Einstein, F-69621 Villeurbanne, France.
authors: Vanessa
authors: V
authors: Eyraud
authors: INRA, INSA-Lyon, Université de Lyon, UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, Bat. Louis-Pasteur, 20 av. Albert Einstein, F-69621 Villeurbanne, France.
authors: Lamis
authors: L
authors: Karaki
authors: Department of Chemical Biology, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
authors: Florenz
authors: F
authors: Sasse
authors: Biology of Caenorhabditis elegans Facility, CNRS UMS 3421, Campus de la Doua, 16 rue Dubois, 69622 Villeurbanne, France; Université Claude Bernard Lyon 1, 43 bd du 11 Novembre, F-69622 Villeurbanne, France.
authors: Maïté
authors: M
authors: Carre-Pierrat
authors: Centre for Orthopaedic Research, School of Surgery, University of Western Australia, Perth, Australia; Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
authors: An
authors: A
authors: Qin
authors: Centre for Orthopaedic Research, School of Surgery, University of Western Australia, Perth, Australia.
authors: Ming H
authors: MH
authors: Zheng
authors: Chinese Ministry of Agriculture - CABI Joint Laboratory for Biosafety, Yuanmingyuan Western Road 2, CN - 100193 Beijing, PR China.
authors: Stefan
authors: S
authors: Toepfer
authors: INRA, INSA-Lyon, Université de Lyon, UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, Bat. Louis-Pasteur, 20 av. Albert Einstein, F-69621 Villeurbanne, France.
authors: Catherine
authors: C
authors: Sivignon
authors: INRA, INSA-Lyon, Université de Lyon, UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, Bat. Louis-Pasteur, 20 av. Albert Einstein, F-69621 Villeurbanne, France.
authors: Corinne
authors: C
authors: Royer
authors: INRA, INSA-Lyon, Université de Lyon, UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, Bat. Louis-Pasteur, 20 av. Albert Einstein, F-69621 Villeurbanne, France.
authors: Pedro
authors: P
authors: Da Silva
authors: INRA, INSA-Lyon, Université de Lyon, UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, Bat. Louis-Pasteur, 20 av. Albert Einstein, F-69621 Villeurbanne, France. Electronic address: Frederic.Gressent@supagro.inra.fr.
authors: Frédéric
authors: F
authors: Gressent
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D001517:Coleoptera / Q000187:drug effects; D004331:Drosophila melanogaster / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry; D007313:Insecta / Q000187:drug effects*; D007305:Insecticide Resistance; D061986:MCF-7 Cells; D051379:Mice; D008969:Molecular Sequence Data; D010010:Osteoclasts / Q000187:drug effects; D010575:Pesticides / Q000737:chemistry / Q000633:toxicity*; D010940:Plant Proteins / Q000737:chemistry / Q000633:toxicity*; D012639:Seeds / Q000737:chemistry; D018675:Toxicity Tests; D025262:Vacuolar Proton-Translocating ATPases / Q000737:chemistry*
chemical_list: D019476:Insect Proteins; D010575:Pesticides; D010940:Plant Proteins; D025262:Vacuolar Proton-Translocating ATPases
keywords: Biopesticide; Insect; Non-target effects; PA1b; Pea Albumin 1 subunit b; V-ATPase