title: Analyzing time-series microarray data reveals key genes in spinal cord injury.
abstract: Although many scholars have utilized high-throughput microarrays to delineate gene expression patterns after spinal cord injury (SCI), no study has evaluated gene changes in raphe magnus (RM) and somatomotor cortex (SMTC), two areas in brain primarily affected by SCI. In present study, we aimed to analyze the differentially expressed genes (DEGs) of RM and SMTC between SCI model and sham injured control at 4, 24 h, 7, 14, 28 days, and 3 months using microarray dataset GSE2270 downloaded from gene expression omnibus and unpaired significance analysis of microarray method. Protein-protein interaction (PPI) network was constructed for DEGs at crucial time points and significant biological functions were enriched using DAVID. The results indicated that more DEGs were identified at 14 days in RM and at 4 h/3 months in SMTC after SCI. In the PPI network for DEGs at 14 days in RM, interleukin 6, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), FBJ murine osteosarcoma viral oncogene homolog (FOS), tumor necrosis factor, and nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) were the top 5 hub genes; In the PPI network for DEGs at 3 months in SMTC, the top 5 hub genes were ubiquitin B, Ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), FOS, Janus kinase 2 and vascular endothelial growth factor A. Hedgehog and Wnt signaling pathways were the top 2 significant pathways in RM. These hub DEGs and pathways may be underlying therapeutic targets for SCI.
authors: Department of Neurosurgery, Chengdu Military General Hospital, No. 270 Rongdu Road, Chengdu, 610083, Sichuan, People's Republic of China.
authors: Xun
authors: X
authors: Xia
authors: Bo
authors: B
authors: Qu
authors: Yuan
authors: Y
authors: Ma
authors: Li-Bin
authors: LB
authors: Yang
authors: Hai-Dong
authors: HD
authors: Huang
authors: Jing-Ming
authors: JM
authors: Cheng
authors: Tao
authors: T
authors: Yang
authors: Bin
authors: B
authors: Kong
authors: En-Yu
authors: EY
authors: Liu
authors: Kai
authors: K
authors: Zhao
authors: Wei-Qi
authors: WQ
authors: He
authors: Xue-Min
authors: XM
authors: Xing
authors: Liang
authors: L
authors: Liang
authors: Ke-Xia
authors: KX
authors: Fan
authors: Hao-Dong
authors: HD
authors: Sun
authors: Hu-Tian
authors: HT
authors: Zhou
authors: Lin
authors: L
authors: Cheng
authors: Jian-Wen
authors: JW
authors: Gu
authors: Yong-Qin
authors: YQ
authors: Kuang