title: Phage-displayed peptides as capture antigens in an innovative assay for Taenia saginata-infected cattle.
abstract: Bovine cysticercosis is detected during the routine post mortem examination of carcasses by visual inspection (knife and eye method). However, the sensitivity of this procedure is several times lower than immunoassays, even when it is performed by qualified professionals. In the present study, a new generation capture antigens were screened from a phage display peptide library using antibodies from Taenia saginata-infected animals. Eight phage clones were selected, and one, Tsag 3 (VHTSIRPRCQPRAITPR), produced similar results to the T. saginata metacestode crude antigen (TsCa) when used as a capture antigen in an ELISA. The phage-displayed peptides competed with TsCa for binding sites, reducing the reactivity by approximately 30 %. Alanine scanning indicated that proline, arginine, and serine are important residues for antibody binding. Tsag 1 (HFYQITWLPNTFPAR), the most frequent affinity-selected clone, and Tsag 6 (YRWPSTPSASRQATL) shared similarity with highly conserved proteins from the Taeniidae family with known immunogenicity. Due to their epitopic or mimotopic properties, these affinity-selected phages could contribute to the rational design of an ante mortem immunodiagnosis method for bovine cysticercosis, as well as an epitope-based vaccine to interrupt the taeniosis/cysticercosis complex.
authors: Departamento de Patologia Básica, UFPR, Curitiba, PR, Brazil.
authors: Rafaela L
authors: RL
authors: Fogaça
authors: Janaína
authors: J
authors: Capelli-Peixoto
authors: Isabel B
authors: IB
authors: Yamanaka
authors: Rodrigo P M
authors: RP
authors: de Almeida
authors: João Carlos D
authors: JC
authors: Muzzi
authors: Mariangela
authors: M
authors: Borges
authors: Alvimar J
authors: AJ
authors: Costa
authors: Carlos
authors: C
authors: Chávez-Olortegui
authors: Vanete
authors: V
authors: Thomaz-Soccol
authors: Larissa M
authors: LM
authors: Alvarenga
authors: Juliana
authors: J
authors: de Moura
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D061505:Cell Surface Display Techniques / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics; D041202:Taenia saginata / Q000276:immunology*; D013622:Taeniasis / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011994:Recombinant Proteins