title: Development and characterization of microsatellite loci for Ocotea species (Lauraceae) threatened with extinction.
abstract: The Atlantic rainforest species Ocotea catharinensis, Ocotea odorifera, and Ocotea porosa have been extensively harvested in the past for timber and oil extraction and are currently listed as threatened due to overexploitation. To investigate the genetic diversity and population structure of these species, we developed 8 polymorphic microsatellite markers for O. odorifera from an enriched microsatellite library by using 2 dinucleotide repeats. The microsatellite markers were tested for cross-amplification in O. catharinensis and O. porosa. The average number of alleles per locus was 10.2, considering all loci over 2 populations of O. odorifera. Observed and expected heterozygosities for O. odorifera ranged from 0.39 to 0.93 and 0.41 to 0.92 across populations, respectively. Cross-amplification of all loci was successfully observed in O. catharinensis and O. porosa except 1 locus that was found to lack polymorphism in O. porosa. Combined probabilities of identity in the studied Ocotea species were very low ranging from 1.0 x 10-24 to 7.7 x 10-24. The probability of exclusion over all loci estimated for O. odorifera indicated a 99.9% chance of correctly excluding a random nonparent individual. The microsatellite markers described in this study have high information content and will be useful for further investigations on genetic diversity within these species and for subsequent conservation purposes.
authors: Diretoria de Pesquisa, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rio de Janeiro, RJ, Brasil eline@cncflora.net.
authors: E M
authors: EM
authors: Martins
authors: Diretoria de Pesquisa, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors: G
authors: G
authors: Martinelli
authors: Laboratorio Ecotono, Universidad Nacional de Río Negro, Sede Andina, Universidad Nacional del Comahue, Bariloche, Río Negro, Argentina.
authors: M P
authors: MP
authors: Arbetman
authors: GeneCology Research Centre, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
authors: R W
authors: RW
authors: Lamont
authors: Centro Nacional de Pesquisa de Agrobiologia, Empresa Brasileira de Pesquisa Agropecuária, Seropédica, RJ, Brasil.
authors: J L
authors: JL
authors: Simões-Araújo
authors: GeneCology Research Centre, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
authors: D
authors: D
authors: Powell
authors: Centro de Engenharia Genética e Biologia Molecular, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors: M
authors: M
authors: Ciampi-Guillardi
authors: Laboratório de Etnoecologia e Manejo Florestal, Departamento de Ciência Animal, Universidade Federal Rural do Semi-árido, Mossoró, RN, Brasil.
authors: C
authors: C
authors: Baldauf
authors: Diretoria de Pesquisa, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
authors: A
authors: A
authors: Quinet
authors: Centro Nacional de Pesquisa de Agrobiologia, Empresa Brasileira de Pesquisa Agropecuária, Seropédica, RJ, Brasil.
authors: P
authors: P
authors: Galisa
authors: GeneCology Research Centre, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
authors: A
authors: A
authors: Shapcott
mesh_terms: D000483:Alleles; D003247:Conservation of Natural Resources; D056727:Endangered Species; D056426:Genetic Loci; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D006579:Heterozygote; D018895:Microsatellite Repeats; D027444:Ocotea / Q000145:classification / Q000235:genetics*; D065947:Rainforest; D013045:Species Specificity
chemical_list: D005819:Genetic Markers