title: [Investigation on mosquitoes and mosquito-borne viruses in Dehong prefecture, Yunnan province, 2007 and 2010].
abstract: OBJECTIVE
  To investigate the distribution patterns of mosquito and mosquito-borne viruses in Dehong prefecture, Yunnan province, China.
  
  
  METHODS
  Mosquito samples were collected using the mosquito traps from five counties of Dehong prefecture on July, 2007 and 2010. Mosquito were cell cultured for viral isolation, and positive isolates were identified using RT-PCR and sequence analysis.
  
  
  RESULTS
  A total of 43 634 mosquito comprised of 29 species representing six genera were collected. Culex tritaeniorhynchus and Anopheles sinensis comprised 78.69% and 14.77% of the total. Six strains of viruses were isolated from the mosquito pools. RT-PCR and phylogenetic analysis revealed three strains from Cx. tritaeniorhynchus, identified as genotype I Japanese encephalitis virus (JEV). One strain was identified from Cx. tritaeniorhynchus, as Getah virus (GETV). Two strains isolated from Cx. tritaeniorhynchus and Anopheles vagus were identified as Culex pipiens pallens Densovirus (CppDNV).
  
  
  CONCLUSIONS
  Cx. tritaeniorhynchus had been the major species of mosquito and mainly transmitting vector of mosquito-borne viruses in Dehong prefecture. Genotype I JEV, GETV and CppDNV were the vectors causing transmission of mosquito-borne diseases in this area. Data from phylogenetic analysis showed that these newly discovered isolates seemed to have had close relationship with those viruses previously circulating in Yunnan and other provinces of China.
authors: Yunnan Institute of Endemic Diseases Control and Prevention, Dali 671000, China.
authors: Yun
authors: Y
authors: Feng
authors: Yunnan Institute of Endemic Diseases Control and Prevention, Dali 671000, China. Email: zhangHL715@163.com.
authors: Hailin
authors: H
authors: Zhang
authors: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors: Shihong
authors: S
authors: Fu
authors: Yunnan Institute of Endemic Diseases Control and Prevention, Dali 671000, China.
authors: Weihong
authors: W
authors: Yang
authors: Yunnan Institute of Endemic Diseases Control and Prevention, Dali 671000, China.
authors: Yuzhen
authors: Y
authors: Zhang
authors: Yunnan Provincial Institute of Parasitic Diseases.
authors: Piyu
authors: P
authors: Wang
authors: Dehong Prefecture Center for Disease Control and Prevention.
authors: Jie
authors: J
authors: Yang
authors: Ruili Center for Disease Control and Prevention.
authors: Yonghua
authors: Y
authors: Liu
authors: Dehong Prefecture Center for Disease Control and Prevention.
authors: Chaoliang
authors: C
authors: Dong
authors: Ruili Center for Disease Control and Prevention.
authors: Shi
authors: S
authors: Li
authors: Dehong Prefecture Center for Disease Control and Prevention.
authors: Baosen
authors: B
authors: Zhang
authors: Ruili Center for Disease Control and Prevention.
authors: Zhengliu
authors: Z
authors: Yin
authors: Yunnan Institute of Endemic Diseases Control and Prevention, Dali 671000, China.
authors: Xingqi
authors: X
authors: Dong
authors: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
authors: Huanyu
authors: H
authors: Wang
authors: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention. Email: gdliang@hotmail.com.
authors: Guodong
authors: G
authors: Liang
mesh_terms: D000524:Alphavirus / Q000302:isolation & purification; D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000302:isolation & purification*; D002681:China; D009033:Culicidae / Q000821:virology*; D004199:Disease Vectors / Q000145:classification; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification