title: Evidence for distinct electron transfer processes in terminal oxidases from different origin by means of protein film voltammetry.
abstract: Cytochrome aa3 from Paracoccus denitrificans and cytochrome ba3 from Thermus thermophilus, two distinct members of the heme-copper oxidase superfamily, were immobilized on electrodes modified with gold nanoparticles. This procedure allowed us to achieve direct electron transfer between the enzyme and the gold nanoparticles and to obtain evidence for different electrocatalytic properties of the two enzymes. The pH dependence and thermostability reveal that the enzymes are highly adapted to their native environments. These results suggest that evolution resulted in different solutions to the common problem of electron transfer to oxygen.
authors: Chimie de la Matière Complexe UMR 7140, Laboratoire de Bioélectrochimie et Spectroscopie, CNRS-Université de Strasbourg , 1 rue Blaise Pascal, 67070 Strasbourg, France.
authors: Thomas
authors: T
authors: Meyer
authors: Frédéric
authors: F
authors: Melin
authors: Hao
authors: H
authors: Xie
authors: Iris
authors: I
authors: von der Hocht
authors: Sylvia K
authors: SK
authors: Choi
authors: Mohamed R
authors: MR
authors: Noor
authors: Hartmut
authors: H
authors: Michel
authors: Robert B
authors: RB
authors: Gennis
authors: Tewfik
authors: T
authors: Soulimane
authors: Petra
authors: P
authors: Hellwig
mesh_terms: D003573:Cytochrome b Group / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D010231:Paracoccus denitrificans / Q000201:enzymology; D016963:Thermus thermophilus
chemical_list: D003573:Cytochrome b Group; D004800:Enzymes, Immobilized; D006046:Gold; C058557:cytochrome ba3; D003576:Electron Transport Complex IV