title: Time-effect relationship of extracts from ginseng, notoginseng and chuanxiong on vascular endothelial cells senescence.
abstract: OBJECTIVE
  To observe the time-effect relation of extracts from ginseng, notoginseng and chuanxiong on angiotensin II (Ang II)-induced senescence of vascular endothelial cells and explore the feature of Chinese medicine against vascular diseases.
  
  
  METHODS
  Human umbilical vein endothelial cells (HUVECs) cultured in vitro were stimulated with 10(-6) mol/L AngII to induce cell senescence, which were divided into 4 groups, the blank control group, the Ang II model group, the extracts group and the telmisartan group. The β-gal was used to identify senescence of cells, the cell counting kit-8 method was applied to assess the cell viability, the cell function was examined with the level of endothelial nitric oxide synthase (eNOS) and the flow cytometry was used for analyzing the cell cycle changes.
  
  
  RESULTS
  Compared with the control cells, the cells positive for β-gal staining was significantly increased in the Ang II model group, and showed cell cycle arrest at G0/G1 phase with decreased S and G2/M phase cell percentage, eNOS expression and cell viability (P<0.05). The extracts and telmisartan treatment of Ang II-induced cells resulted in decreased β-gal positive cells with a reduction in G0/G1 phase cells and an increasing in S, G2/M phase cells and eNOS expression (P<0.05). At 24 h, the extracts were more effective than telmisartan (P<0.05); while telmisartan was more effective at 48 h (P<0.05).
  
  
  CONCLUSIONS
  Extracts from ginseng, notoginseng and chuanxiong can delay Ang II-induced aging of HUVECs and may play an important role in early senescence.
authors: The Cardiology Department of Chongqing Hospital of Traditional Chinese Medicine and Chongqing the First Hospital, Chongqing, 400021, China.
authors: Ming
authors: M
authors: Wang
authors: Yan
authors: Y
authors: Lei
mesh_terms: D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D053723:Panax notoginseng / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D052250:Nitric Oxide Synthase Type III; D001616:beta-Galactosidase