title: [Distribution of serum antibodies against human papillomavirus 16 and 18 among high-risk women to cervical cancer].
abstract: OBJECTIVE
  To explore the distribution of serum antibodies against human papillomavirus (HPV) 16/18 among women at high-risk for cervical cancer.
  
  
  METHODS
  All women when tested positive for anyone of the cervical cancer screening programs, from Xinmi county of Henan province in 2011, were recruited as the subjects of this study. Cervical exfoliated cells were collected, using cervical brush for HPV DNA testing, and 10 ml venous blood was drawn for HPV-16, 18 serum antibodies testing, by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Among the 952 women under study, 230 cases (24.2%)showed HPV DNA positive, with positivity rates of HPV16 and 18 L1 virus-like particle (VLP)antibodies as 23.2% and 6.5%, respectively. The overall positivity rate of any type of HPV16, 18 VLP antibodies was 26.8% . Geometric means of HPV16, 18 VLP antibody titers were 79.1 (Yangshengtang Unit,YU/ml) and 125.0(YU/ml). Positivity rate of HPV16 antibody was significantly associated with age, viral load of HPV DNA, and cervical lesion severity (P < 0.05). Seropositivity of HPV18 was also increasing with the increase of viral load (P < 0.01) with different cervical lesion significantly showing different titer of HPV18 antibody (P < 0.01). Based on the results of HPV DNA detection among the two years of study, women with HPV persistent infection showed significant higher positive rate of HPV16/18 antibodies than women who did not have HPV infection or emerging infection (P < 0.001). When comparing to those women without HPV infection, the ones with transient infection showed higher seropositivity rates on both HPV16 antibodies and titer of HPV16 antibody (P < 0.001).
  
  
  CONCLUSIONS
  Seroprevalence rates on HPV16 and 18 among the unvaccinated high-risk women in Henan were high. Prevalence of both HPV16 and 18 antibodies were correlated with age, viral load, cervical lesion and history of infection.Women with high viral load, high grade cervical lesion or history of infection would more likely to be seropositive.
authors: Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China; Department of Epidemiology, West China School of Public Health, Sichuan University.
authors: Mandong
authors: M
authors: Fei
authors: Department of Epidemiology, West China School of Public Health, Sichuan University.
authors: Jiayuan
authors: J
authors: Li
authors: Institute of Labor and Environmental Hygiene, Chongqing Medical University.
authors: Jingchang
authors: J
authors: Du
authors: Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors: Jia
authors: J
authors: You
authors: Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors: Shaokai
authors: S
authors: Zhang
authors: Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors: Wei
authors: W
authors: He
authors: Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors: Leni
authors: L
authors: Kang
authors: Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors: Fanghui
authors: F
authors: Zhao
authors: Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors: Youlin
authors: Y
authors: Qiao
authors: Women and Children Hospital in Xinmi City of Henan Province.
authors: Yuzhi
authors: Y
authors: Si
authors: Women and Children Hospital in Xinmi City of Henan Province.
authors: Xiaoping
authors: X
authors: Fan
authors: Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. Email: chenwen@cicams.ac.cn.
authors: Wen
authors: W
authors: Chen
mesh_terms: D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood*; D002681:China / Q000453:epidemiology; D005260:Female; D052162:Human papillomavirus 16 / Q000276:immunology; D052161:Human papillomavirus 18 / Q000276:immunology; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology*; D016036:Seroepidemiologic Studies; D002583:Uterine Cervical Neoplasms / Q000821:virology*
chemical_list: D000914:Antibodies, Viral