title: Effect of Peripheral Administration of Kisspeptin-10 on Dynamic LH Secretion in Prepubertal Ewes.
abstract: The aim of the present study was to clarify the effect of kisspeptin-10 on LH secretion in prepubertal ewes. In experiment 1, prepubertal ewes fitted with indwelling jugular catheters were randomly assigned to receive 0, 0.5, 1 or 2 mg of kisspeptin-10 dissolved in saline, and serial blood samples were collected at 15-min intervals for 180 min to analyze the response curves of LH after injection. In experiment 2, prepubertal ewes fitted with indwelling jugular catheters were injected with 0 or 1 mg of kisspeptin-10 dissolved in saline and the injection was repeated 3 times at 1 h interval and serial blood samples were collected at 15-min intervals for 210 min to analyze the response curves of LH after injection. The results showed that single intravenous administration of 0.5, 1 and 2 mg of kisspeptin-10 all could significantly increased LH secretion in prepubertal ewes, and the effect of 1 and 2 mg of kisspeptin-10 on LH secretion was higher than that of 0.5 mg group. The results also showed that repeated intravenous administration of kisspeptin-10 could effectively increase LH secretion and repeated administration did not influence the effect of kisspeptin-10 on LH secretion in prepubertal ewe. In conclusion, the present study indicated that single or repeated intravenous administration of kisspeptin-10 could effectively increase LH secretion in prepubertal ewes.
authors: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China ; College of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou, 121001, China .
authors: Jun
authors: J
authors: Wang
authors: College of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou, 121001, China .
authors: Lei
authors: L
authors: Sun
authors: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China ; Xuelong Industrial Group, Dalian, 116002, China .
authors: Tao
authors: T
authors: Zhang
authors: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
authors: Haizhu
authors: H
authors: Zhou
authors: College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
authors: Yujie
authors: Y
authors: Lou
keywords: Kisspeptin-10; LH; Prepubertal Ewes