title: Oncogenic role of long noncoding RNA AF118081 in anti-benzo[a]pyrene-trans-7,8-dihydrodiol-9,10-epoxide-transformed 16HBE cells.
abstract: Lung cancer is the leading cause of cancer deaths and remains an important public health problem worldwide. Long noncoding RNAs (lncRNAs) are newly identified regulators of tumorigenesis and tumor progression. However, the role of lncRNAs in lung cancer induced by environmental carcinogens remains largely unknown. In this study, an lncRNA microarray was used to compare the expression profiles of malignantly transformed 16HBE cells (16HBE-T) induced with anti-benzo[a]pyrene-trans-7,8-diol-9,10-epoxide (anti-BPDE) and normal 16HBE cells (16HBE-N). Using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR), lncRNA AF118081 was identified as the most significantly overexpressed lncRNA in 16HBE-T cells, lung cancer cells, and patient samples. Cell proliferation, colony formation, apoptosis, migration, and invasion were assayed in 16HBE-T cells following the knockdown of lncRNA AF118081 with small interfering RNA. AF118081 knockdown inhibited cell growth and tumor invasion. An in vivo (nude mouse) model was then used to assay tumor growth, and the downregulation of AF118081 clearly suppressed tumor growth, consistent with the results of the in vitro assays. Together, these findings identify a new oncogenic lncRNA, lncRNA AF118081, in malignantly transformed 16HBE cells. This enhances our understanding of lncRNAs as important regulatory elements in chemical carcinogenesis and potential targets of lung cancer therapies.
authors: Institute for Chemical Carcinogenesis, State Key Laboratory for Respiratory Diseases, Guangzhou Medical University, Guangzhou 510182, PR China.
authors: Qiaoyuan
authors: Q
authors: Yang
authors: Institute for Chemical Carcinogenesis, State Key Laboratory for Respiratory Diseases, Guangzhou Medical University, Guangzhou 510182, PR China.
authors: Shaozhu
authors: S
authors: Zhang
authors: Institute for Chemical Carcinogenesis, State Key Laboratory for Respiratory Diseases, Guangzhou Medical University, Guangzhou 510182, PR China.
authors: Huanying
authors: H
authors: Liu
authors: Institute for Chemical Carcinogenesis, State Key Laboratory for Respiratory Diseases, Guangzhou Medical University, Guangzhou 510182, PR China.
authors: Jianjun
authors: J
authors: Wu
authors: Department of Thoracic Surgery, General Hospital of Guangzhou Military Command of Chinese People's Liberation Army, Guangzhou 510010, PR China.
authors: Enwu
authors: E
authors: Xu
authors: Institute for Chemical Carcinogenesis, State Key Laboratory for Respiratory Diseases, Guangzhou Medical University, Guangzhou 510182, PR China.
authors: Baoying
authors: B
authors: Peng
authors: Institute for Chemical Carcinogenesis, State Key Laboratory for Respiratory Diseases, Guangzhou Medical University, Guangzhou 510182, PR China. Electronic address: jiangyiguo@vip.163.com.
authors: Yiguo
authors: Y
authors: Jiang
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001980:Bronchi / Q000166:cytology / Q000187:drug effects; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000503:physiopathology; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D009857:Oncogenes / Q000502:physiology*; D034622:RNA Interference / Q000187:drug effects; D062085:RNA, Long Noncoding / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D020545:Respiratory Mucosa / Q000166:cytology* / Q000187:drug effects
chemical_list: D002273:Carcinogens; D062085:RNA, Long Noncoding; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
keywords: Anti-BPDE; Long noncoding RNA; Lung cancer