title: Neurite growth acceleration of adult Dorsal Root Ganglion neurons illuminated by low-level Light Emitting Diode light at 645 nm.
abstract: The effect of a 645 nm Light Emitting Diode (LED) light irradiation on the neurite growth velocity of adult Dorsal Root Ganglion (DRG) neurons with peripheral axon injury 4-10 days before plating and without previous injury was investigated. The real amount of light reaching the neurons was calculated by taking into account the optical characteristics of the light source and of media in the light path. The knowledge of these parameters is essential to be able to compare results of the literature and a way to reduce inconsistencies. We found that 4 min irradiation of a mean irradiance of 11.3 mW/cm(2) (corresponding to an actual irradiance reaching the neurons of 83 mW/cm(2)) induced a 1.6-fold neurite growth acceleration on non-injured neurons and on axotomized neurons. Although the axotomized neurons were naturally already in a rapid regeneration process, an enhancement was found to occur while irradiating with the LED light, which may be promising for therapy applications. Dorsal Root Ganglion neurons (A) without previous injury and (B) subjected to a conditioning injury.
authors: Inserm U1051., INM, Hôpital St Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.|Biolux Medical, 7 Place de la Comédie, 34000, Montpellier, France.
authors: Marion
authors: M
authors: Burland
authors: Inserm U1051., INM, Hôpital St Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.|CNRS, IES, UMR 5214, 34000, Montpellier, France.|Université de Nîmes, Place Gabriel Péri, 30000, Nîmes, France.
authors: Lambert
authors: L
authors: Paris
authors: Inserm U1051., INM, Hôpital St Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.
authors: Patrice
authors: P
authors: Quintana
authors: Biolux Medical, 7 Place de la Comédie, 34000, Montpellier, France.
authors: Jean-Michel
authors: JM
authors: Bec
authors: Inserm U1051., INM, Hôpital St Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.
authors: Lucie
authors: L
authors: Diouloufet
authors: Inserm U1051., INM, Hôpital St Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.
authors: Chamroeun
authors: C
authors: Sar
authors: Inserm U1051., INM, Hôpital St Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.
authors: Hassan
authors: H
authors: Boukhaddaoui
authors: CNRS, IES, UMR 5214, 34000, Montpellier, France.
authors: Benoit
authors: B
authors: Charlot
authors: Hôpital São Lucas PUCRS, Av. Ipiranga 6690, Porto Alegre, RS, Brazil.
authors: Jefferson
authors: J
authors: Braga Silva
authors: Inserm U1051., INM, Hôpital St Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.
authors: Michel
authors: M
authors: Chammas
authors: Inserm U1051., INM, Hôpital St Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.
authors: Victor
authors: V
authors: Sieso
authors: Inserm U1051., INM, Hôpital St Eloi, 80 rue Augustin Fliche, 34091, Montpellier Cedex 5, France.|Univ. Montpellier, INM, Inserm U1051, 34000, Montpellier, France.
authors: Jean
authors: J
authors: Valmier
authors: CNRS, IES, UMR 5214, 34000, Montpellier, France.|Université de Nîmes, Place Gabriel Péri, 30000, Nîmes, France.
authors: Fabrice
authors: F
authors: Bardin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects*; D028022:Low-Level Light Therapy / Q000295:instrumentation / Q000379:methods*; D008159:Lumbar Vertebrae; D051379:Mice; D008853:Microscopy; D016501:Neurites / Q000473:pathology / Q000502:physiology / Q000528:radiation effects*; D011897:Random Allocation; D012584:Sciatic Nerve / Q000293:injuries*; D011984:Sensory Receptor Cells / Q000473:pathology / Q000502:physiology / Q000528:radiation effects*; D013057:Spectrum Analysis; D014741:Video Recording
keywords: DRG; LED; dorsal root ganglion neuron; light emitting diode; low-level light therapy; neurite growth; peripheral nerve injury