title: Association of the 3'UTR FOXO3a polymorphism rs4946936 with an increased risk of childhood acute lymphoblastic leukemia in a Chinese population.
abstract: OBJECTIVE
  FOXO3a is an essential tumor suppressor that regulates the mechanisms of tumorigenesis and leukemogenesis. FOXO3a polymorphisms have not been reported previously associated with an increased risk for childhood acute lymphoblastic leukemia (ALL). In this study, the rs4946936 polymorphism located in the 3'UTR of FOXO3a was selected to evaluate its relationship with a risk for ALL in Chinese children.
  
  
  METHODS
  Questionnaires, SNaPshot genotyping, real-time PCR, cell transfection and dual luciferase reporter assays were used in this study.
  
  
  RESULTS
  Parental alcohol consumption and whether the child's house had been painted had significantly different distributions among the cases and controls. In addition, the C to T mutation was significantly associated with the risk for ALL. We determined that FOXO3a expression levels in patients with the CT or TT genotype were significantly higher than those of patients with the CC genotype. The T allele significantly increased the expression levels in luciferase assays and affected the binding affinity of miR-223 to the FOXO3a 3'UTR.
  
  
  CONCLUSIONS
  Rs4946936 in FOXO3a was highly associated with an increased risk of childhood ALL in a Chinese population.
authors: Department of Hematology and Oncology, Nanjing Children's Hospital Affiliated with Nanjing Medical University, Nanjing, China.
authors: Yaping
authors: Y
authors: Wang
authors: Li
authors: L
authors: Zhou
authors: Jing
authors: J
authors: Chen
authors: Jie
authors: J
authors: Li
authors: Lulu
authors: L
authors: He
authors: Peng
authors: P
authors: Wu
authors: Meilin
authors: M
authors: Wang
authors: Na
authors: N
authors: Tong
authors: Zhengdong
authors: Z
authors: Zhang
authors: Yongjun
authors: Y
authors: Fang
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D016022:Case-Control Studies; D002675:Child, Preschool; D002681:China; D017931:DNA Primers; D005260:Female; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D020413:3' Untranslated Regions; D017931:DNA Primers; C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors