title: Silencing of hypoxia-inducible factor-1β induces anti-tumor effects in hepatoma cell lines under tumor hypoxia.
abstract: Dimerization of hypoxia-inducible factor-1 beta (HIF-1β) [aryl hydrocarbon receptor nuclear translocator (ARNT)] with HIF-1α is involved in various aspects of cancer biology, including proliferation and survival under hypoxic conditions. We investigated the in vitro mechanism by which silencing of HIF-1β leads to the suppression of tumor cell growth and cellular functions. Various hepatocellular carcinoma (HCC) cell lines (Huh-7, Hep3B, and HepG2) were transfected with small interfering RNA (siRNA) against HIF-1β (siHIF-1β) and cultured under hypoxic conditions (1% O2 for 24 h). The expression levels of HIF-1β, HIF-1α, and growth factors were examined by immunoblotting. Tumor growth was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and tumor activity was measured by terminal deoxynucleotidyl transferase dUTP nick end labeling, tumor cell invasion, and migration assays. Under hypoxic conditions, silencing of HIF-1β expression suppressed tumor cell growth and regulated the expression of tumor growth-related factors, such as vascular endothelial growth factor, epidermal growth factor, and hepatocyte growth factor. Suppression of tumor cell invasion and migration was also demonstrated in HIF-1β-silenced HCC cell lines. Silencing of HIF-1β expression may induce anti-tumor effects under hypoxic conditions in HCC cell lines.
authors: Brain Korea 21 plus project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors: Sung Hoon
authors: SH
authors: Choi
authors: Brain Korea 21 plus project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors: Ae Ri
authors: AR
authors: Chung
authors: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea; Yonsei Liver Cancer Special Clinic, Yonsei University Health System, Seoul, Korea; Liver Cirrhosis Clinical Research Center, Yonsei University Health System, Seoul, Korea.
authors: Wonseok
authors: W
authors: Kang
authors: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea; Yonsei Liver Cancer Special Clinic, Yonsei University Health System, Seoul, Korea; Liver Cirrhosis Clinical Research Center, Yonsei University Health System, Seoul, Korea.
authors: Jun Yong
authors: JY
authors: Park
authors: Department of Premed, Yonsei University College of Medicine, Seoul, Korea.
authors: Mi Sol
authors: MS
authors: Lee
authors: Department of Premed, Yonsei University College of Medicine, Seoul, Korea.
authors: Shin Won
authors: SW
authors: Hwang
authors: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea; Yonsei Liver Cancer Special Clinic, Yonsei University Health System, Seoul, Korea; Liver Cirrhosis Clinical Research Center, Yonsei University Health System, Seoul, Korea.
authors: Do Young
authors: DY
authors: Kim
authors: Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea; Yonsei Liver Cancer Special Clinic, Yonsei University Health System, Seoul, Korea; Liver Cirrhosis Clinical Research Center, Yonsei University Health System, Seoul, Korea.
authors: Seung Up
authors: SU
authors: Kim
authors: Brain Korea 21 plus project for Medical Science, Yonsei University College of Medicine, Seoul, Korea; Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea; Yonsei Liver Cancer Special Clinic, Yonsei University Health System, Seoul, Korea; Liver Cirrhosis Clinical Research Center, Yonsei University Health System, Seoul, Korea.
authors: Sang Hoon
authors: SH
authors: Ahn
authors: Brain Korea 21 plus project for Medical Science, Yonsei University College of Medicine, Seoul, Korea; Liver Cirrhosis Clinical Research Center, Yonsei University Health System, Seoul, Korea.
authors: Seungtaek
authors: S
authors: Kim
authors: Brain Korea 21 plus project for Medical Science, Yonsei University College of Medicine, Seoul, Korea; Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea; Yonsei Liver Cancer Special Clinic, Yonsei University Health System, Seoul, Korea; Liver Cirrhosis Clinical Research Center, Yonsei University Health System, Seoul, Korea.
authors: Kwang-Hyub
authors: KH
authors: Han
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000235:genetics* / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D015151:Immunoblotting; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator