title: Nuclear expression of phosphorylated focal adhesion kinase is associated with poor prognosis in human colorectal cancer.
abstract: OBJECTIVE
  To determine whether phosphorylated focal adhesion kinase (P-FAK) has prognostic value in colorectal cancer (CRC) and to test whether it has any association with Tensin 4 (TNS4) expression.
  
  
  METHODS
  P-FAK expression was assessed using immunohistochemistry in 462 CRC cases arrayed on a tissue microarray. P-FAK and TNS4 expression were assessed by immunohistochemistry in 40 cases of paired primary colorectal cancer and corresponding hepatic metastases.
  
  
  RESULTS
  Nuclear P-FAK expression was observed in 44% of studied cases. Positive nuclear P-FAK expression was associated with shorter disease-specific survival in univariate (p=0.005) and multivariate analysis (p=0.016). P-FAK expression was greater in metastases than the primary tumours (p<0.001) and showed significant association with nuclear TNS4 (p<0.001) in metastases.
  
  
  CONCLUSIONS
  P-FAK expression is an independent prognostic marker in CRC. The present data suggest that the FAK signalling pathway may interact with TNS4, a known oncogene in CRC.
authors: Division of Pathology, School of Molecular Medical Sciences, University of Nottingham, Queen's Medical Centre, Nottingham, U.K. Division of Pathology, Faculty of Medicine, University of Tibah, Medina, Saudi Arabia.
authors: Abdulkader
authors: A
authors: Albasri
authors: Division of Pathology, School of Molecular Medical Sciences, University of Nottingham, Queen's Medical Centre, Nottingham, U.K.
authors: Wakkas
authors: W
authors: Fadhil
authors: Division of Gastrointestinal Surgery, Nottingham Digestive Diseases Centre NIHR Biomedical Research Unit, Nottingham University Hospitals, Queen's Medical Centre, Nottingham, U.K.
authors: John H
authors: JH
authors: Scholefield
authors: Academic Department of Clinical Oncology, School of Molecular Medical Sciences, City Hospital Campus, University of Nottingham, Nottingham, U.K.
authors: Lindy G
authors: LG
authors: Durrant
authors: Division of Pathology, School of Molecular Medical Sciences, University of Nottingham, Queen's Medical Centre, Nottingham, U.K. Mohammad.ilyas@nottingham.ac.uk.
authors: Mohammad
authors: M
authors: Ilyas
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002467:Cell Nucleus / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism*; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis; D008875:Middle Aged; D009362:Neoplasm Metastasis; D010766:Phosphorylation; D011379:Prognosis; D000070876:Tensins; D046888:Tissue Array Analysis
chemical_list: D008840:Microfilament Proteins; C000603371:TNS4 protein, human; D000070876:Tensins; D051416:Focal Adhesion Protein-Tyrosine Kinases
keywords: Colorectal cancer; TNS4 (CTEN); focal adhesion kinase; prognosis