title: Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by EngineeredEscherichia coli.
abstract: This study was designed to determine whether whole cells or crude enzyme extracts are more effective for preparative-scale ketone reductions by dehydrogenases as well as learning which cofactor regeneration scheme is most effective. Based on results from three representative ketone substrates (an α-fluoro-β-keto ester, abis-trifluoromethylated acetophenone, and a symmetrical β-diketone), our results demonstrate that several nicotinamide cofactor regeneration strategies can be applied to preparative-scale dehydrogenase-catalyzed reactions successfully.
authors: Department of Chemistry, University of Florida , 126 Sisler Hall, Gainesville, Florida 32611, United States.
authors: Dimitri
authors: D
authors: Dascier
authors: Codexis, Inc., Penobscot Drive 200, Redwood City, California 94063, United States.
authors: Spiros
authors: S
authors: Kambourakis
authors: Department of Chemistry, University of Florida , 126 Sisler Hall, Gainesville, Florida 32611, United States.
authors: Ling
authors: L
authors: Hua
authors: Codexis, Inc., Penobscot Drive 200, Redwood City, California 94063, United States.
authors: J David
authors: JD
authors: Rozzell
authors: Department of Chemistry, University of Florida , 126 Sisler Hall, Gainesville, Florida 32611, United States.
authors: Jon D
authors: JD
authors: Stewart