title: Blood profile and histology in oral infections associated with diabetes.
abstract: BACKGROUND
  We aimed to investigate the relationship between blood profile and histologic findings in both apical periodontitis (AP) and periodontal disease (PD) associated with diabetes.
  
  
  METHODS
  Wistar rats (N = 80) were assigned to the following 8 groups: control, AP, PD, AP associated with PD, diabetes, diabetes with AP, diabetes with PD, and diabetes with AP and PD. Diabetes mellitus (DM) was induced with streptozotocin, AP was induced by exposure to the oral environment, and PD was induced using periodontal ligature. After 30 days, blood samples were collected, and the rats were euthanized. Subsequently, the maxillae were processed for light microscopy. Hematologic examinations were conducted to determine the total number of erythrocytes and leukocytes, erythrocyte constant, and blood glucose level. One-way analysis of variance and Kruskal-Wallis tests were used for statistical analysis, and the significance was set at P < .05.
  
  
  RESULTS
  A significant correlation was found between the histologic findings and blood parameters.
  
  
  CONCLUSIONS
  In conclusion, diabetes accelerated the development and progression of AP and PD in the rats and caused an increase in the average erythrocyte volume as well as the leukocyte and neutrophil counts. Oral infections increase the total number of leukocytes, the number of neutrophils and lymphocytes, and blood glucose concentrations in DM rats.
authors: Department of Endodontics, Araçatuba Dental School, Universidade Estadual Paulista, São Paulo, Brazil. Electronic address: lucianocintra@foa.unesp.br.
authors: Luciano Tavares Angelo
authors: LT
authors: Cintra
authors: Department of Endodontics, Araçatuba Dental School, Universidade Estadual Paulista, São Paulo, Brazil.
authors: Aguinaldo Cândido
authors: AC
authors: da Silva Facundo
authors: Department of Endodontics, Araçatuba Dental School, Universidade Estadual Paulista, São Paulo, Brazil.
authors: Annelise Katrine Carrara
authors: AK
authors: Prieto
authors: Department of Basic Science, Araçatuba Dental School, Universidade Estadual Paulista, São Paulo, Brazil.
authors: Dóris Hissako
authors: DH
authors: Sumida
authors: Department of Clinic and Surgery and Animal Reproduction, Araçatuba Veterinary Medicine, Universidade Estadual Paulista, São Paulo, Brazil.
authors: Luís Gustavo
authors: LG
authors: Narciso
authors: Department of Clinic and Surgery and Animal Reproduction, Araçatuba Veterinary Medicine, Universidade Estadual Paulista, São Paulo, Brazil.
authors: Suely Regina
authors: SR
authors: Mogami Bomfim
authors: Department of Periodontics, Maringá Dental School, Universidade Estadual Maringá, Paraná, Brazil.
authors: Cleverson
authors: C
authors: Oliveira e Silva
authors: Department of Endodontics, Araçatuba Dental School, Universidade Estadual Paulista, São Paulo, Brazil.
authors: Eloi
authors: E
authors: Dezan-Júnior
authors: Department of Endodontics, Araçatuba Dental School, Universidade Estadual Paulista, São Paulo, Brazil.
authors: João Eduardo
authors: JE
authors: Gomes-Filho
mesh_terms: D016301:Alveolar Bone Loss / Q000097:blood / Q000473:pathology; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003789:Dental Pulp Exposure / Q000150:complications / Q000473:pathology; D003790:Dental Pulp Necrosis / Q000150:complications / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000150:complications; D018450:Disease Progression; D004906:Erythrocyte Count; D004909:Erythrocyte Indices; D004911:Erythrocyte Volume; D007958:Leukocyte Count; D018655:Lymphocyte Count; D008297:Male; D009504:Neutrophils / Q000473:pathology; D010485:Periapical Periodontitis / Q000097:blood* / Q000150:complications / Q000473:pathology; D010510:Periodontal Diseases / Q000097:blood* / Q000150:complications / Q000473:pathology; D010513:Periodontal Ligament / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D013311:Streptozocin
keywords: Apical periodontitis; blood count; diabetes; oral infections; periodontal disease