title: Identification and characterization of microRNAs in small brown planthopper (Laodephax striatellus) by next-generation sequencing.
abstract: MicroRNAs (miRNAs) are endogenous non-coding small RNAs that regulate gene expression at the post-transcriptional level and are thought to play critical roles in many metabolic activities in eukaryotes. The small brown planthopper (Laodephax striatellus Fallén), one of the most destructive agricultural pests, causes great damage to crops including rice, wheat, and maize. However, information about the genome of L. striatellus is limited. In this study, a small RNA library was constructed from a mixed L. striatellus population and sequenced by Solexa sequencing technology. A total of 501 mature miRNAs were identified, including 227 conserved and 274 novel miRNAs belonging to 125 and 250 families, respectively. Sixty-nine conserved miRNAs that are included in 38 families are predicted to have an RNA secondary structure typically found in miRNAs. Many miRNAs were validated by stem-loop RT-PCR. Comparison with the miRNAs in 84 animal species from miRBase showed that the conserved miRNA families we identified are highly conserved in the Arthropoda phylum. Furthermore, miRanda predicted 2701 target genes for 378 miRNAs, which could be categorized into 52 functional groups annotated by gene ontology. The function of miRNA target genes was found to be very similar between conserved and novel miRNAs. This study of miRNAs in L. striatellus will provide new information and enhance the understanding of the role of miRNAs in the regulation of L. striatellus metabolism and development.
authors: College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, China.
authors: Guoyan
authors: G
authors: Zhou
authors: College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, China.
authors: Tingzhang
authors: T
authors: Wang
authors: College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, China.
authors: Yonggen
authors: Y
authors: Lou
authors: College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, China.
authors: Jia'an
authors: J
authors: Cheng
authors: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors: Hengmu
authors: H
authors: Zhang
authors: College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, China.
authors: Jian-Hong
authors: JH
authors: Xu
mesh_terms: D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D018556:Crops, Agricultural / Q000469:parasitology; D005786:Gene Expression Regulation; D015723:Gene Library; D006430:Hemiptera / Q000235:genetics* / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation
chemical_list: D035683:MicroRNAs