title: Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae.
abstract: Oligomeric sugars in the liquid fraction of hot water-pretreated rice straw are more amenable to membrane process than monomeric sugars, as lower pressure is required. Following membrane process was employed: nanofiltration (NF) concentration → (dilution → NF concentration) × 2 times → enzymatic hydrolysis (EH) → ultrafiltration (UF) permeation [
  
  
  CONCLUSIONS
  NF for recovery of oligomeric sugars, dilution and NF for removal of low molecular weight fermentation inhibitors, UF for removal of high molecular weight fermentation inhibitors and recovery of monomeric sugars after EH]. This process provided the liquid fraction containing 111.4 g L(-1) of sugars, corresponding to 681.0mM as monomeric sugars, from the original liquid fraction (181.1mM monomeric sugars). Concentrations of low molecular weight fermentation inhibitors, acetic and formic acids, were decreased to 24% and 48%, respectively. Xylose-fermenting recombinant Saccharomyces cerevisiae produced 34.5 ± 2.2 g L(-1) ethanol from the 0.8 times liquid fraction (76% of theoretical yield).
authors: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 675-8501, Japan.
authors: Kengo
authors: K
authors: Sasaki
authors: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 675-8501, Japan.
authors: Yota
authors: Y
authors: Tsuge
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors: Daisuke
authors: D
authors: Sasaki
authors: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 675-8501, Japan.
authors: Tomohisa
authors: T
authors: Hasunuma
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors: Takatoshi
authors: T
authors: Sakamoto
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors: Yuri
authors: Y
authors: Sakihama
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors: Chiaki
authors: C
authors: Ogino
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan; Biomass Engineering Program, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea. Electronic address: akondo@kobe-u.ac.jp.
authors: Akihiko
authors: A
authors: Kondo
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008567:Membranes, Artificial; D008970:Molecular Weight; D012275:Oryza / Q000737:chemistry*; D010539:Permeability; D011134:Polysaccharides / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014462:Ultrafiltration / Q000379:methods*; D014866:Waste Products; D014994:Xylose
chemical_list: D002241:Carbohydrates; D008567:Membranes, Artificial; D011134:Polysaccharides; D014866:Waste Products; D000431:Ethanol; C007916:hemicellulose; D014994:Xylose
keywords: Ethanol; Hemicellulose; Nanofiltration; Rice straw; Ultrafiltration