title: Integrated transcriptional and metabolic profiling in human endotoxemia.
abstract: In this meta-study, we aimed to integrate biological insights gained from two levels of -omics analyses on the response to systemic inflammation induced by lipopolysaccharide in humans. We characterized the interplay between plasma metabolite compositions and transcriptional response of leukocytes through integration of transcriptomics with plasma metabonomics. We hypothesized that the drastic changes in the immediate environment of the leukocytes might have an adaptive effect on shaping their transcriptional response in conjunction with the initial inflammatory stimuli. Indeed, we observed that leukocytes, most notably, tune the activity of lipid- and protein-associated processes at the transcriptional level in accordance with the fluctuations in metabolite compositions of surrounding plasma. A closer look into the transcriptional control of only metabolic pathways uncovered alterations in bioenergetics and defenses against oxidative stress closely associated with mitochondrial dysfunction and shifts in energy production observed during inflammatory processes.
authors: *Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway; †Department of Surgery, Rutgers-Robert Wood Johnson Medical School, New Brunswick; and ‡Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, New Jersey.
authors: Kubra
authors: K
authors: Kamisoglu
authors: Steve E
authors: SE
authors: Calvano
authors: Susette M
authors: SM
authors: Coyle
authors: Siobhan A
authors: SA
authors: Corbett
authors: Ioannis P
authors: IP
authors: Androulakis
mesh_terms: D000293:Adolescent; D000328:Adult; D019446:Endotoxemia / Q000378:metabolism* / Q000503:physiopathology; D004731:Endotoxins / Q000737:chemistry*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry; D008297:Male; D055442:Metabolome; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress; D014158:Transcription, Genetic; D059467:Transcriptome; D055815:Young Adult
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides