title: Evaluation of anti-urolithiatic effect of aqueous extract of Bryophyllum pinnatum (Lam.) leaves using ethylene glycol-induced renal calculi.
abstract: Materials and Methods : Thirty-six Wistar male rats were randomly divided into six equal groups. Group A animals received distilled water for 28 days. Group B to group F animals received 1% v/v ethylene glycol in distilled water for 28 days and group B served as ethylene glycol control. Groups C and D (preventive groups) received aqueous extract of leaves of B. pinnatum 50 and 100 mg/kg intraperitoneally, respectively for 28 days. Groups E and F (treatment groups) received aqueous extract of leaves of B. pinnatum 50 and 100 mg/kg intraperitoneally, respectively from 15(th) to 28(th) day. On days 0 and 28, 24 hrs urine samples were collected for urinary volume and urinary oxalate measurement. On day 28, blood was collected for serum creatinine and blood urea level monitoring. All animals were sacrificed and kidneys were removed, weighed, and histopathologically evaluated for calcium oxalate crystals deposition.
  
  
  RESULTS
  Administration of aqueous extract of leaves of B. pinnatum reduced urine oxalate level ‎ significantly, as compared with Group B (p<0.001). Serum creatinine and blood urea level were ‎ improved significantly in all aqueous extract of leaves of B. pinnatum-treated groups. Relative ‎ kidney weight and calcium oxalate depositions were found significantly reduced in animals ‎ received ABP as compared with Group B (p<0.001). ‎
  
  
  CONCLUSIONS
  B. pinnatum is effective in prevention and treatment of ethylene glycol-induced urolithiasis.
authors: Department of Pharmacology, Government Medical College and Sir Takhtsinhji General ‎ Hospital, Bhavnagar-364001 (Gujarat), India ‎
authors: Apexa Bhanuprasad
authors: AB
authors: Shukla
authors: Department of Pharmacology, Government Medical College and Sir Takhtsinhji General ‎ Hospital, Bhavnagar-364001 (Gujarat), India ‎
authors: Divyesh Rasikbhai
authors: DR
authors: Mandavia
authors: Department of Pharmacology, Government Medical College and Sir Takhtsinhji General ‎ Hospital, Bhavnagar-364001 (Gujarat), India ‎
authors: Manish Jasmatbhai
authors: MJ
authors: Barvaliya
authors: ‎ Department of Pathology, Government Medical College and Sir Takhtsinhji General Hospital, ‎ Bhavnagar-364001 (Gujarat), India ‎
authors: Seema Natvarlal
authors: SN
authors: Baxi
authors: Department of Pharmacology, Government Medical College and Sir Takhtsinhji General ‎ Hospital, Bhavnagar-364001 (Gujarat), India ‎
authors: Chandrabhanu Rajkishore
authors: CR
authors: Tripathi
keywords: Bryophyllum pinnatum (Lam.) Calcium oxalate; Ethylene glycol; Renal calculi