title: Assays for necroptosis and activity of RIP kinases.
abstract: Necrosis is a primary form of cell death in a variety of human pathologies. The deleterious nature of necrosis, including its propensity to promote inflammation, and the relative lack of the cells displaying necrotic morphology under physiologic settings, such as during development, have contributed to the notion that necrosis represents a form of pathologic stress-induced nonspecific cell lysis. However, this notion has been challenged in recent years by the discovery of a highly regulated form of necrosis, termed regulated necrosis or necroptosis. Necroptosis is now recognized by the work of multiple labs, as an important, drug-targetable contributor to necrotic injury in many pathologies, including ischemia-reperfusion injuries (heart, brain, kidney, liver), brain trauma, eye diseases, and acute inflammatory conditions. In this review, we describe the methods to analyze cellular necroptosis and activity of its key mediator, RIP1 kinase.
authors: Department of Developmental, Molecular & Chemical Biology, Tufts University School of Medicine, Boston, Massachusetts, USA.
authors: Alexei
authors: A
authors: Degterev
authors: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors: Wen
authors: W
authors: Zhou
authors: Department of Developmental, Molecular & Chemical Biology, Tufts University School of Medicine, Boston, Massachusetts, USA.
authors: Jenny L
authors: JL
authors: Maki
authors: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA. Electronic address: junying_yuan@hms.harvard.edu.
authors: Junying
authors: J
authors: Yuan
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001681:Biological Assay / Q000379:methods*; D006801:Humans; D009336:Necrosis / Q000201:enzymology*; D028861:Nuclear Pore Complex Proteins / Q000096:biosynthesis* / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D053422:Receptor-Interacting Protein Serine-Threonine Kinases / Q000235:genetics; D015427:Reperfusion Injury / Q000201:enzymology* / Q000235:genetics
chemical_list: C119724:AGFG1 protein, human; D028861:Nuclear Pore Complex Proteins; D016601:RNA-Binding Proteins; C570693:MLKL protein, human; D011494:Protein Kinases; C119705:RIPK3 protein, human; D053422:Receptor-Interacting Protein Serine-Threonine Kinases
keywords: Apoptosis; Cell death; MLKL; Necroptosis; Necrosis; Necrosome; RIP1; RIP3