title: Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis.
abstract: The well-studied plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) synthesizes the biotechnologically important polysaccharide xanthan gum, which is also regarded as a virulence factor in plant interactions. In Xcc, sugars like glucose are utilized as a source to generate energy and biomass for growth and pathogenicity. In this study, we used [1-(13)C]glucose as a tracer to analyze the fluxes in the central metabolism of the bacterium growing in a minimal medium. (13)C-Metabolic flux analysis based on gas chromatography-mass spectrometry (GC-MS) confirmed the prevalent catabolic role of the Entner-Doudoroff pathway. Comparative nuclear magnetic resonance (NMR)-based isotopologue profiling of a mutant deficient in glycolysis gave evidence for a moderate flux via glycolysis in the wild-type. In addition to reconfirming the Entner-Doudoroff pathway as a catabolic main route, this approach affirmed a numerically minor but important flux via the pentose phosphate pathway.
authors: Abteilung für Proteom- und Metabolomforschung, Fakultät für Biologie, Universität Bielefeld, Bielefeld, Germany. frank@CeBiTec.Uni-Bielefeld.DE.
authors: Sarah
authors: S
authors: Schatschneider
authors: Claudia
authors: C
authors: Huber
authors: Heiko
authors: H
authors: Neuweger
authors: Tony Francis
authors: TF
authors: Watt
authors: Alfred
authors: A
authors: Pühler
authors: Wolfgang
authors: W
authors: Eisenreich
authors: Christoph
authors: C
authors: Wittmann
authors: Karsten
authors: K
authors: Niehaus
authors: Frank-Jörg
authors: FJ
authors: Vorhölter
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D050260:Carbohydrate Metabolism; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D009010:Monte Carlo Method; D019906:Nuclear Magnetic Resonance, Biomolecular; D010427:Pentose Phosphate Pathway; D016959:Xanthomonas campestris / Q000235:genetics / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D005947:Glucose