title: A desaturase gene involved in the formation of 1,14-nonadecadiene in Synechococcus sp. strain PCC 7002.
abstract: The marine cyanobacterium Synechococcus sp. strain PCC 7002 synthesizes two alkenes, 1-nonadecene and 1,14-nonadecadiene. Whereas the genetic basis for the biosynthesis of the terminal double bond in both alkenes has been characterized, the origin of the internal double bond in 1,14-nonadecadiene has not. In this study, we demonstrate that a gene encoding an uncharacterized desaturase is involved in the formation of the internal double bond of 1,14-nonadecadiene. Further, at low temperatures, the desaturase gene is essential for growth, and in wild-type cells the levels of 1,14-nonadecadiene increase relative to that of cells grown at 38°C. These data suggest that 1,14-nonadecadiene plays a role in responding to cold stress.
authors: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors: Daniel
authors: D
authors: Mendez-Perez
authors: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors: Nicolaus A
authors: NA
authors: Herman
authors: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, Wisconsin, USA pfleger@engr.wisc.edu.
authors: Brian F
authors: BF
authors: Pfleger
mesh_terms: D000475:Alkenes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D053898:Biosynthetic Pathways; D003080:Cold Temperature; D017931:DNA Primers / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008055:Lipids / Q000032:analysis; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D013312:Stress, Physiological; D046940:Synechococcus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000502:physiology
chemical_list: D000475:Alkenes; D001426:Bacterial Proteins; D017931:DNA Primers; D008055:Lipids; D010088:Oxidoreductases