title: Next generation ligand binding assays-review of emerging real-time measurement technologies.
abstract: Over the last few years, numerous ligand binding assay technologies that utilize real-time measurement have been introduced; however, an assemblage and evaluation of these technologies has not previously been published. Herein, we describe six emerging real-time measurement technologies: Maverick™, MX96 SPR™, NanoDLSay™, AMMP®/ViBE®, SoPrano™, and two Lab-on-a-Chip (LoC) microfluidic devices. The development stage gate of these technologies ranges from pre-commercial to commercially available. Due to the novelty, the application and utility of some of the technologies regarding bioanalysis are likely to evolve but it is our hope that this review will provide insight into the direction the development of real-time measurement technologies is moving and the vision of those that are taking us there. Following the technology discussions, a comprehensive summary table is presented.
authors: Pfizer Global Research and Development, Groton, Connecticut, USA, stephanie.fraser@pfizer.com.
authors: Stephanie
authors: S
authors: Fraser
authors: Mark
authors: M
authors: Cameron
authors: Edward
authors: E
authors: O'Connor
authors: Martin
authors: M
authors: Schwickart
authors: Michael
authors: M
authors: Tanen
authors: Mark
authors: M
authors: Ware
mesh_terms: D000162:Acoustics / Q000295:instrumentation; D000818:Animals; D001665:Binding Sites; D015195:Drug Design; D004867:Equipment Design; D006801:Humans; D007700:Kinetics; D008024:Ligands; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D008904:Miniaturization; D036103:Nanotechnology / Q000295:instrumentation; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D020349:Surface Plasmon Resonance / Q000295:instrumentation; D013678:Technology, Pharmaceutical / Q000295:instrumentation*
chemical_list: D008024:Ligands; D004364:Pharmaceutical Preparations