title: Olive oil mill wastewaters: phenolic content characterization during degradation by Coriolopsis gallica.
abstract: Olive mill wastewaters (OMW) pose a serious environmental concern owing to high polyphenol content. Decolorization and degradation of phenolic compounds (PC) by Coriolopsis gallica was demonstrated in our laboratory as a potential biotreatment of OMW in solid and liquid media. High performance liquid chromatography coupled to electrospray time-of-flight mass spectrometry was used to analyze the evolution of the main phenolic compounds during the C. gallica biodegradation process. Amongst total the compounds characterized in methanolic extracts of OMW, 12 were unknown, 15 were from different polyphenolic families, and 27 were other non-phenolic compounds. The evolution of PC content during the degradation process indicated that, despite the complexity of the OMW phenolic fraction, C. gallica was able to grow on OMW-based media using PC as sources of carbon and energy, particularly acids, alcohols, lignans and flavones. Complete dephenolization of OMW was obtained.
authors: Laboratory of Enzyme Engineering and Microbiology, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Route de Soukra Km 4,5, BP 1173, 3038 Sfax, Tunisia.
authors: Dalel
authors: D
authors: Daâssi
authors: Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, Italy; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Fuentenueva s/n, E-18071 Granada, Spain; Functional Food Research and Development Center, Health Science Technological Park, Avenida del Conocimiento s/n, E-18100 Granada, Spain.
authors: Jesus
authors: J
authors: Lozano-Sánchez
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Fuentenueva s/n, E-18071 Granada, Spain; Functional Food Research and Development Center, Health Science Technological Park, Avenida del Conocimiento s/n, E-18100 Granada, Spain.
authors: Isabel
authors: I
authors: Borrás-Linares
authors: Laboratory of Soil Biology, University of Neuchatel, Rue Emile Argand 11, CH-2009 Neuchatel, Switzerland.
authors: Lassaad
authors: L
authors: Belbahri
authors: University of Aberdeen, Institute of Biological and Environmental Science, Department of Plant and Soil Science, Cruickshank Building, Aberdeen AB24 3UU, Scotland, UK.
authors: Steve
authors: S
authors: Woodward
authors: Laboratory of Enzyme Engineering and Microbiology, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Route de Soukra Km 4,5, BP 1173, 3038 Sfax, Tunisia.
authors: Héla
authors: H
authors: Zouari-Mechichi
authors: Laboratory of Enzyme Engineering and Microbiology, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Route de Soukra Km 4,5, BP 1173, 3038 Sfax, Tunisia. Electronic address: tahar.mechichi@enis.rnu.tn.
authors: Tahar
authors: T
authors: Mechichi
authors: Laboratory of Enzyme Engineering and Microbiology, Ecole Nationale d'Ingénieurs de Sfax, University of Sfax, Route de Soukra Km 4,5, BP 1173, 3038 Sfax, Tunisia.
authors: Moncef
authors: M
authors: Nasri
authors: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Fuentenueva s/n, E-18071 Granada, Spain; Functional Food Research and Development Center, Health Science Technological Park, Avenida del Conocimiento s/n, E-18100 Granada, Spain.
authors: Antonio
authors: A
authors: Segura-Carretero
mesh_terms: D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D055453:Coriolaceae / Q000378:metabolism*; D013058:Mass Spectrometry; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry* / Q000378:metabolism; D059808:Polyphenols / Q000032:analysis; D062065:Wastewater / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D000069463:Olive Oil; D010938:Plant Oils; D059808:Polyphenols; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Biodegradation; Coriolopsis gallica; HPLC–ESI–TOF–MS; Laccase; Olive mill wastewaters; Polyphenolic compounds