title: Analysis of regioisomers of polyunsaturated triacylglycerols in marine matrices by HPLC/HRMS.
abstract: Natural sources of triacylglycerols containing ω-3 fatty acids are of particular interest due to their protective role against several human diseases. However, as it has been well ascertained, the position of the ω-3 fatty acid on the triacylglycerol backbone influences how digestion occurs. In particular, occurrence at the sn-2 position allows optimal intestinal absorption conditions. The analytical protocol for regioisomer characterisation of fatty acids in a triacylglycerol usually requires the use of stereospecific lipases before instrumental identification. In this paper, we propose a more direct instrumental determination of triacylglycerol composition along with sn-2 positional identification of the fatty acids constituents by Liquid Chromatography-High Resolution Mass Spectrometry. Different intensities of product signals obtained in MS(2) and MS(3) experiments were used to define an interpretative scheme able to rationalise the stereochemistry of the TAGs. Marine matrices like tuna and algae oils have been studied in detail, their triacylglycerols identified and sn-2 positional arrangement of fatty acid constituents assessed.
authors: Molecular Biotechnology and Health Sciences Department, University of Torino, Via P. Giuria 5, 10125 Torino, Italy.
authors: Claudio
authors: C
authors: Baiocchi
authors: Molecular Biotechnology and Health Sciences Department, University of Torino, Via P. Giuria 5, 10125 Torino, Italy.
authors: Claudio
authors: C
authors: Medana
authors: Molecular Biotechnology and Health Sciences Department, University of Torino, Via P. Giuria 5, 10125 Torino, Italy. Electronic address: federica.dalbello@unito.it.
authors: Federica
authors: F
authors: Dal Bello
authors: Molecular Biotechnology and Health Sciences Department, University of Torino, Via P. Giuria 5, 10125 Torino, Italy.
authors: Valeria
authors: V
authors: Giancotti
authors: Molecular Biotechnology and Health Sciences Department, University of Torino, Via P. Giuria 5, 10125 Torino, Italy.
authors: Riccardo
authors: R
authors: Aigotti
authors: Molecular Biotechnology and Health Sciences Department, University of Torino, Via P. Giuria 5, 10125 Torino, Italy.
authors: Daniela
authors: D
authors: Gastaldi
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015525:Fatty Acids, Omega-3 / Q000032:analysis*; D005395:Fish Oils / Q000737:chemistry*; D008049:Lipase / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D013696:Temperature; D014280:Triglycerides / Q000032:analysis*; D014413:Tuna
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D014280:Triglycerides; D008049:Lipase
keywords: High resolution mass spectrometry; Marine oils; Omega-3 fatty acids; Regioisomer characterisation