title: Camelina sativa defatted seed meal contains both alkyl sulfinyl glucosinolates and quercetin that synergize bioactivity.
abstract: Camelina sativa L. Crantz is under development as a novel oilseed crop, yet bioefficacy of camelina phytochemicals is unknown. Defatted camelina seed meal contains two major aliphatic glucosinolates (GSLs), glucoarabin (9-(methylsulfinyl)nonylglucosinolate; GSL 9) and glucocamelinin (10-(methylsulfinyl)decylglucosinolate; GSL 10), with traces of a third, 11(methylsulfinyl)undecylglucosinolate and several flavonoids, mostly quercetin glycosides. In Hepa1c1c7 cells, hydrolyzed GSLs (hGSLs) 9 and 10 upregulated the phase II detoxification enzyme quinone reductase (NQO1), with no effect on cytochrome P450 (CYP) 1A1 activity. Isobologram graphs revealed synergy of NQO1 induction for a combination of hGSL 9 and quercetin. These findings suggest that defatted camelina seed meal should be evaluated for anticancer activity, similar to broccoli and other Brassicaceae family members. Interestingly, synergy of NQO1 induction was also seen for physiologically relevant doses of sulforaphane (SF) and quercetin, two key bioactives present in broccoli. This suggests that SF within broccoli may be more potent than purified SF.
authors: Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign , 905 S. Goodwin Avenue, Urbana, Illinois 61801, United States.
authors: Nilanjan
authors: N
authors: Das
authors: Mark A
authors: MA
authors: Berhow
authors: Donato
authors: D
authors: Angelino
authors: Elizabeth H
authors: EH
authors: Jeffery
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000494:pharmacology; D019607:Brassicaceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004357:Drug Synergism; D005223:Fats / Q000032:analysis; D005961:Glucosinolates / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D011794:Quercetin / Q000032:analysis / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D005223:Fats; D005961:Glucosinolates; D010936:Plant Extracts; D011794:Quercetin
keywords: NQO1; camelina; glucosinolate; quercetin; sulforaphane; synergy