title: Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop.
abstract: Cryotop is a carrier that has been used successfully in the cryopreservation of human spermatozoa. Here, we explored a novel method to vitrify human spermatozoa without cryoprotective agents (CPAs) using Cryotop. Spermatozoa from 21 Normozoospermic patients were collected and vitrified without CPAs or with sucrose in small volume using Cryotop. The sperm recovery rate, motility, viability, chromatin damage and DNA fragmentation were assessed. No significant difference was observed in the sperm recovery rate and motility rate between the spermatozoa cryopreserved without CPAs and with sucrose. The post-thawed spermatozoa cryopreserved without CPAs had a higher viability and lower damage to sperm chromatin and DNA than those cryopreserved with sucrose. These results suggest that small numbers of human spermatozoa can be successfully vitrified without CPAs using Cryotop.
authors: Reproductive Medicine Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.|Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: Y
authors: Y
authors: Chen
authors: Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: L
authors: L
authors: Li
authors: Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: Y
authors: Y
authors: Qian
authors: Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: C
authors: C
authors: Xu
authors: Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: Y
authors: Y
authors: Zhu
authors: Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: H
authors: H
authors: Huang
authors: Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: F
authors: F
authors: Jin
authors: Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors: Y
authors: Y
authors: Ye
mesh_terms: D002843:Chromatin / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D053938:DNA Fragmentation; D006801:Humans; D008297:Male; D055101:Semen Analysis; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D002843:Chromatin
keywords: Cryoprotective agents; Cryotop; spermatozoa; sucrose; vitrification