title: Determination of chrysotoxine in rat plasma by liquid chromatography-tandem mass spectrometry and its application to a rat pharmacokinetic study.
abstract: Chrysotoxine (CTX), a naturally occurring bibenzyl compound isolated from Dendrobium species, has been reported to have neuroprotective effects. To evaluate its pharmacokinetics in rats, a rapid, sensitive and specific high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method has been developed and validated for the quantification of CTX in rat plasma. Samples were pretreated using a simple liquid-liquid extraction with ethyl acetate and the chromatographic separation was performed on a C18 column with acetonitrile-water (90:10, v/v) as the mobile phase. CTX and the internal standard (wogonin) were detected using a tandem mass spectrometer in positive multiple reaction monitoring mode. Method validation revealed excellent linearity over the range 0.5-1000 ng/mL together with satisfactory intra- and inter-day precision, accuracy and recovery. Stability testing showed that CTX spiked into rat plasma was stable for 8 h at room temperature, for up to two weeks at -20 °C, and during three freeze-thaw cycles. Extracted samples were also observed to be stable over 24 h in an auto-sampler. The method was successfully used to investigate the pharmacokinetic profile of CTX after oral (100 mg/kg) and intravenous (25 mg/kg) administration in rats. CTX showed rapid excretion and low bioavailability in rats.
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China.
authors: Jingjing
authors: J
authors: Fan
authors: Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, 210009, China.
authors: Li
authors: L
authors: Guan
authors: Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, 210009, China.
authors: Zeqi
authors: Z
authors: Kou
authors: Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, 210009, China.
authors: Feng
authors: F
authors: Feng
authors: School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong, China. Electronic address: ybzhang@hku.hk.
authors: Yanbo
authors: Y
authors: Zhang
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China. Electronic address: liuwenyuan8506@163.com.
authors: Wenyuan
authors: W
authors: Liu
mesh_terms: D000085:Acetates; D000818:Animals; D001632:Bibenzyls / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000085:Acetates; D001632:Bibenzyls; C555497:chrysotoxine; C007650:ethyl acetate
keywords: Bioavailability; Chrysotoxine; HPLC–MS/MS; Method validation; Pharmacokinetics