title: Sensitive impedimetric biosensor for direct detection of diazinon based on lipases.
abstract: Two novel impedimetric biosensors for highly sensitive and rapid quantitative detection of diazinon in aqueous medium were developed using two types of lipase, from Candida Rugosa (microbial source) (CRL) and from porcine pancreas (animal source) (PPL) immobilized on functionalized gold electrode. Lipase is characterized to specifically catalyze the hydrolysis of ester functions leading to the transformation of diazinon into diethyl phosphorothioic acid (DETP) and 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP). The developed biosensors both presented a wide range of linearity up to 50 μM with a detection limit of 10 nM for Candida Rugosa biosensor and 0.1 μM for porcine pancreas biosensor. A comparative study was carried out between the two biosensors and results showed higher efficiency of Candida Rugosa sensor. Moreover, it presented good accuracy and reproducibility, had very good storage and multiple use stability for 25 days when stored at 4°C.
authors: UMR 5280, Institut des Sciences Analytiques, Université de Lyon Villeurbanne, France ; Laboratory of Environmental Engineering, Faculty of Engineering Sciences, University of Annaba Annaba, Algeria.
authors: Nedjla
authors: N
authors: Zehani
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine Kiev, Ukraine.
authors: Sergei V
authors: SV
authors: Dzyadevych
authors: Laboratory of Environmental Engineering, Faculty of Engineering Sciences, University of Annaba Annaba, Algeria.
authors: Rochdi
authors: R
authors: Kherrat
authors: UMR 5280, Institut des Sciences Analytiques, Université de Lyon Villeurbanne, France.
authors: Nicole J
authors: NJ
authors: Jaffrezic-Renault
keywords: biosensor; diazinon; electrochemical impedance spectroscopy; lipase