title: Protective action of curcumin and nano-curcumin against arsenic-induced genotoxicity in rats in vivo.
abstract: We explored whether nanoformulation of curcumin can cause better protective effect than free curcumin against arsenic-induced genotoxicity. Curcumin-loaded Poly(lactic-co-glycolic acid) nanoparticles (CUR-NP) were prepared by emulsion technique. The CUR-NP were water soluble and showed biphasic release pattern. Rats were divided into 5 groups of 6 each. Group I served as the control. Group II rats were exposed to sodium arsenite (25 ppm) daily through drinking water for 42 days. Groups III, IV and V were maintained as in Group II, however, they were also administered empty nanoparticle, curcumin (100 mg/kg bw) and CUR-NP (100 mg/kg bw), respectively, by oral gavage during the last 14 days of arsenic exposure. On the 43rd day, genotoxic effects were evaluated in bone marrow cells. Arsenic increased chromosomal aberrations, micronuclei formation and DNA damage. Both free curcumin and CUR-NP attenuated these arsenic-mediated genotoxic effects. However, the result suggests that nanoformulation have better protective effect than free curcumin at the same dose level.
authors: Department of Veterinary Pharmacology and Toxicology, Veterinary College and Research Institute, Orathanadu, 614625, Tamil Nadu, India, drpsankarster@gmail.com.
authors: Palanisamy
authors: P
authors: Sankar
authors: Avinash Gopal
authors: AG
authors: Telang
authors: Kalaivanan
authors: K
authors: Ramya
authors: Karunakaran
authors: K
authors: Vijayakaran
authors: Manickam
authors: M
authors: Kesavan
authors: Souvendra Nath
authors: SN
authors: Sarkar
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D018053:Arsenites / Q000633:toxicity*; D001854:Bone Marrow Cells / Q000378:metabolism; D002869:Chromosome Aberrations / Q000187:drug effects; D020552:Comet Assay; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D004249:DNA Damage / Q000187:drug effects*; D019344:Lactic Acid / Q000737:chemistry / Q000627:therapeutic use; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000627:therapeutic use; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017670:Sodium Compounds / Q000633:toxicity*
chemical_list: D018053:Arsenites; D017670:Sodium Compounds; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C017947:sodium arsenite; D003474:Curcumin