title: Remote ischemic postconditioning protects against gastric mucosal lesions in rats.
abstract: OBJECTIVE
  To investigate the protective effects of remote ischemic postconditioning (RIP) against limb ischemia-reperfusion (IR)-induced gastric mucosal injury.
  
  
  METHODS
  Gastric IR was established in male Wistar rats by placing an elastic rubber band under a pressure of 290-310 mmHg on the proximal part of both lower limbs for 3 h followed by reperfusion for 0, 1, 3, 6, 12 or 24 h. RIP was performed using three cycles of 30 s of reperfusion and 30 s of reocclusion of the femoral aortic immediately after IR and before reperfusion for up to 24 h. Rats were randomly assigned to receive IR (n = 36), IR followed by RIP (n = 36), or sham treatment (n = 36). Gastric tissue samples were collected from six animals in each group at each timepoint and processed to determine levels of malondialdehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XOD) and myeloperoxidase (MPO). Additional samples were processed for histologic analysis by hematoxylin and eosin staining. Blood samples were similarly collected to determine serum levels of lactate dehydrogenase (LDH), creatine kinase (CK), tumor necrosis factor (TNF)-α and interleukin (IL)-10.
  
  
  RESULTS
  The pathologic changes in gastric tissue induced by IR were observed by light microscopy. Administration of RIP dramatically reduced the gastric damage score after 6 h of reperfusion (5.85 ± 0.22 vs 7.72 ± 0.43; P < 0.01). In addition, RIP treatment decreased the serum activities of LDH (3.31 ± 0.32 vs 6.46 ± 0.03; P < 0.01), CK (1.94 ± 0.20 vs 4.54 ± 0.19; P < 0.01) and the concentration of TNF-α (53.82 ± 0.85 vs 88.50 ± 3.08; P < 0.01), and elevated the concentration of IL-10 (101.46 ± 5.08 vs 99.77 ± 4.32; P < 0.01) induced by IR at 6 h. Furthermore, RIP treatment prevented the marked elevation in MDA (3.79 ± 0.29 vs 6.39 ± 0.81) content, XOD (7.81 ± 0.75 vs 10.37 ± 2.47) and MPO (0.47 ± 0.05 vs 0.82 ± 0.03) activities, and decrease in SOD (4.95 ± 0.32 vs 3.41 ± 0.38; P < 0.01) activity in the gastric tissue as measured at 6 h.
  
  
  CONCLUSIONS
  RIP provides effective functional protection and prevents cell injury to gastric tissue induced by limb IR via anti-inflammatory and antioxidant actions.
authors: Tao Wang, Department of Anesthesiology, Shuyang People's Hospital, Shuyang 223600, Jiangsu Province, China.
authors: Tao
authors: T
authors: Wang
authors: Tao Wang, Department of Anesthesiology, Shuyang People's Hospital, Shuyang 223600, Jiangsu Province, China.
authors: Ye-Ting
authors: YT
authors: Zhou
authors: Tao Wang, Department of Anesthesiology, Shuyang People's Hospital, Shuyang 223600, Jiangsu Province, China.
authors: Xin-Nian
authors: XN
authors: Chen
authors: Tao Wang, Department of Anesthesiology, Shuyang People's Hospital, Shuyang 223600, Jiangsu Province, China.
authors: An-Xiang
authors: AX
authors: Zhu
authors: Tao Wang, Department of Anesthesiology, Shuyang People's Hospital, Shuyang 223600, Jiangsu Province, China.
authors: Bo-Hua
authors: BH
authors: Wu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D006614:Hindlimb / Q000098:blood supply*; D018836:Inflammation Mediators / Q000097:blood; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D017208:Rats, Wistar; D012039:Regional Blood Flow; D015427:Reperfusion Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors; D014111:Tourniquets
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D018836:Inflammation Mediators
keywords: Gastric ischemia; Limb ischemia-reperfusion; Protection; Rat; Remote ischemic postconditioning