title: CD64 expression is increased in patients with severe acute pancreatitis: clinical significance.
abstract: OBJECTIVE
  Upregulated CD64 expression on neutrophils is the most useful marker for acute bacterial infections and systemic inflammation. However, it is unknown whether CD64 is involved in the pathogenesis of acute pancreatitis (AP). This study was designed to determine whether CD64 is implicated in severe acute pancreatitis (SAP), and thus, is a suitable marker for SAP.
  
  
  METHODS
  SAP was induced in rats with an intraperitoneal injection of L-arginine. CD64 expression in the rat pancreas was determined by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry. Additionally, the CD64 mRNA expression in peripheral blood leukocytes from 21 patients with mild acute pancreatitis (MAP) and 10 patients with SAP was investigated at the time of admission and during remission by qRT-PCR.
  
  
  RESULTS
  CD64 mRNA and protein expression in the pancreas was significantly higher in rats with SAP, compared to the controls. The CD64 expression was higher in the patients with SAP than in the patients with MAP. During remission, CD64 mRNA decreased in both the MAP and SAP patients. The area under the curve of CD64 expression for the detection of SAP was superior to both the Ranson and the Acute Physiology and Chronic Health Evaluation II scores.
  
  
  CONCLUSIONS
  The CD64 level was significantly increased in correlation with the disease severity in SAP and may act as a useful marker for predicting the development of SAP.
authors: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China ; Department of Gastroenterology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors: Hao
authors: H
authors: Zhang
authors: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors: Xian-Long
authors: XL
authors: Ling
authors: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors: Yu-Yun
authors: YY
authors: Wu
authors: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors: Mu-Han
authors: MH
authors: Lü
authors: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors: Hong
authors: H
authors: Guo
authors: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors: Peng-Bin
authors: PB
authors: Zhang
authors: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors: Xiao-Yan
authors: XY
authors: Zhao
authors: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China ; Biomedical Analysis Center, Third Military Medical University, Chongqing, China ; Chongqing Key Laboratory for Diseases Proteomics, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Shi-Ming
authors: SM
authors: Yang
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001120:Arginine / Q000633:toxicity; D005260:Female; D049690:History, Ancient; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010195:Pancreatitis / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D017452:Receptors, IgG / Q000378:metabolism*; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D017452:Receptors, IgG; D001120:Arginine
keywords: CD64; Leukocytes; Severe acute pancreatitis