title: Cytokines Expression and Nitric Oxide Production under Induced Infection to Salmonella Typhimurium in Chicken Lines Divergently Selected for Cutaneous Hypersensitivity.
abstract: In the present study, the impact of Salmonella Typhimurium on cell-mediated immunity (CMI) was investigated in 5 week-old immuno divergent broiler lines selected for the high and low response to phytohemagglutinin-P. The immune response was assessed in peripheral-blood mononuclear cells (PBMCs) induced with Salmonella Typhimurium at different time intervals (0 h, 0.5 h, 2 h, 4 h, 6 h, 12 h and 24 h). The differential mRNA expression patterns of IFN-γ, IL-2 and iNOS were evaluated by quantitative real time PCR. In-vitro production of nitric oxide (NO) was also estimated in the culture supernatant and correlated with iNOS mRNA expression. Present study showed higher production of NO in the high cell-mediated line (HCMI) as compared to the low cell-mediated line (LCMI) upon stimulation with Salmonella Typhimurium. Correspondingly, higher mRNA expression of iNOS and IFN-γ were observed in high response birds (HCMI); but IL-2 was down regulated in this line compared to the low response birds (LCMI). Significantly (p<0.05) higher expression of iNOS, IFN-γ and higher production of NO in high line indicated that the selection for PHA-P response might be employed for increasing the immune competence against Salmonella Typhimurium in chicken flocks.
authors: Avian Physiology and Genetics Division, Salim Ali Centre for Ornithology and Natural History, Anaikatty 641 108, Coimbatore, India .
authors: Rani
authors: R
authors: Singh
authors: Avian Physiology and Genetics Division, Salim Ali Centre for Ornithology and Natural History, Anaikatty 641 108, Coimbatore, India .
authors: Preeti
authors: P
authors: Jain
authors: Avian Physiology and Genetics Division, Salim Ali Centre for Ornithology and Natural History, Anaikatty 641 108, Coimbatore, India .
authors: N K
authors: NK
authors: Pandey
authors: Avian Physiology and Genetics Division, Salim Ali Centre for Ornithology and Natural History, Anaikatty 641 108, Coimbatore, India .
authors: V K
authors: VK
authors: Saxena
authors: Avian Physiology and Genetics Division, Salim Ali Centre for Ornithology and Natural History, Anaikatty 641 108, Coimbatore, India .
authors: M
authors: M
authors: Saxena
authors: Avian Physiology and Genetics Division, Salim Ali Centre for Ornithology and Natural History, Anaikatty 641 108, Coimbatore, India .
authors: K B
authors: KB
authors: Singh
authors: Avian Physiology and Genetics Division, Salim Ali Centre for Ornithology and Natural History, Anaikatty 641 108, Coimbatore, India .
authors: K A
authors: KA
authors: Ahmed
authors: Avian Physiology and Genetics Division, Salim Ali Centre for Ornithology and Natural History, Anaikatty 641 108, Coimbatore, India .
authors: R P
authors: RP
authors: Singh
keywords: Nitric Oxide (NO); Peripheral Blood Mononuclear Cells (PBMCs); Phytohaemagglutinin-P (PHA-P)