title: Screening and determination of sulphonamide residues in bovine milk samples using a flow injection system.
abstract: A new, simple, rapid and sensitive flow injection spectrophotometric method was developed for the screening and determination of sulphonamides in bovine milk samples. The method is based on the condensation of sulphathiazole, sulphamethazine, and sulphadimethoxine with p-dimethylaminobenzaldehyde (p-DAB) in acid medium, in the presence of sodium dodecyl sulphate (SDS), producing a yellow compound (λmax=465 nm). Optimisation of the experimental parameters was performed using a multivariate methodology. The linear range was 90-500 μg/L and the limits of detection and quantification were in the ranges 25-29 μg/L and 84-88 μg/L, respectively. The procedure was applied for the determination of sulphonamide antibiotics in bovine milk samples submitted to a prior extraction procedure based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology. Recoveries of 60.5-70.5% were achieved for milk samples spiked with 0.09, 0.1, 0.2, and 0.3 μg/g of each sulphonamide.
authors: Instituto de Química, Universidade Estadual Paulista (UNESP), P.O. Box 355, Araraquara, CEP 14801-970 SP, Brazil.
authors: Flávio Cesar Bedatty
authors: FCB
authors: Fernandes
authors: Instituto de Química, Universidade Estadual Paulista (UNESP), P.O. Box 355, Araraquara, CEP 14801-970 SP, Brazil.
authors: Aline Santana
authors: AS
authors: Silva
authors: Instituto de Química, Universidade Estadual Paulista (UNESP), P.O. Box 355, Araraquara, CEP 14801-970 SP, Brazil.
authors: José Luiz
authors: JL
authors: Rufino
authors: Instituto de Química, Universidade Estadual Paulista (UNESP), P.O. Box 355, Araraquara, CEP 14801-970 SP, Brazil.
authors: Helena Redigolo
authors: HR
authors: Pezza
authors: Instituto de Química, Universidade Estadual Paulista (UNESP), P.O. Box 355, Araraquara, CEP 14801-970 SP, Brazil. Electronic address: pezza@iq.unesp.br.
authors: Leonardo
authors: L
authors: Pezza
mesh_terms: D000818:Animals; D002417:Cattle; D008892:Milk / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*; D013449:Sulfonamides / Q000032:analysis
chemical_list: D013449:Sulfonamides
keywords: Flow injection analysis; Milk; Screening; Spectrophotometry; Sulphonamides; p-Dimethylaminobenzaldehyde