title: Molecular cytogenetic characterization of multiple intrachromosomal rearrangements in two representatives of the genus Turdus (Turdidae, Passeriformes).
abstract: Turdus rufiventris and Turdus albicollis, two songbirds belonging to the family Turdidae (Aves, Passeriformes) were studied by C-banding, 18S rDNA, as well as the use of whole chromosome probes derived from Gallus gallus (GGA) and Leucopternis albicollis (LAL). They showed very similar karyotypes, with 2n = 78 and the same pattern of distribution of heterochromatic blocks and hybridization patterns. However, the analysis of 18/28S rDNA has shown differences in the number of NOR-bearing chromosomes and ribosomal clusters. The hybridization pattern of GGA macrochromosomes was similar to the one found in songbirds studied by Fluorescent in situ hybridization, with fission of GGA 1 and GGA 4 chromosomes. In contrast, LAL chromosome paintings revealed a complex pattern of intrachromosomal rearrangements (paracentric and pericentric inversions) on chromosome 2, which corresponds to GGA1q. The first inversion changed the chromosomal morphology and the second and third inversions changed the order of chromosome segments. Karyotype analysis in Turdus revealed that this genus has derived characteristics in relation to the putative avian ancestral karyotype, highlighting the importance of using new tools for analysis of chromosomal evolution in birds, such as the probes derived from L. albicollis, which make it possible to identify intrachromosomal rearrangements not visible with the use of GGA chromosome painting solely.
authors: Programa de Pós Graduação em Ciências Biológicas, Universidade Federal do Pampa, São Gabriel, Rio Grande do Sul, Brazil.
authors: Rafael
authors: R
authors: Kretschmer
authors: Programa de Pós Graduação em Ciências Biológicas, Universidade Federal do Pampa, São Gabriel, Rio Grande do Sul, Brazil.
authors: Ricardo José
authors: RJ
authors: Gunski
authors: Programa de Pós Graduação em Ciências Biológicas, Universidade Federal do Pampa, São Gabriel, Rio Grande do Sul, Brazil.
authors: Analía Del Valle
authors: Adel V
authors: Garnero
authors: Laboratório de Cultura de Tecidos e Citogenética, SAMAM, Instituto Evandro Chagas, Ananindeua, Pará, Brazil; Programa de Pós Graduação em Genética e Biologia Molecular, Universidade Federal do Pará, Belém, Pará, Brazil.
authors: Ivanete de Oliveira
authors: Ide O
authors: Furo
authors: Cambridge Resource Centre for Comparative Genomics, Cambridge, United Kingdom.
authors: Patricia C M
authors: PC
authors: O'Brien
authors: Cambridge Resource Centre for Comparative Genomics, Cambridge, United Kingdom.
authors: Malcolm A
authors: MA
authors: Ferguson-Smith
authors: Laboratório de Cultura de Tecidos e Citogenética, SAMAM, Instituto Evandro Chagas, Ananindeua, Pará, Brazil; Programa de Pós Graduação em Genética e Biologia Molecular, Universidade Federal do Pará, Belém, Pará, Brazil; Instiuto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém, Pará, Brazil.
authors: Edivaldo Herculano Corrêa
authors: EH
authors: de Oliveira
mesh_terms: D000818:Animals; D007446:Chromosome Inversion; D020223:Chromosome Painting / Q000379:methods*; D019143:Evolution, Molecular; D059785:Karyotype; D008297:Male; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D020308:Songbirds / Q000145:classification* / Q000235:genetics*
chemical_list: D012337:RNA, Ribosomal, 18S