title: Influence of micropattern width on differentiation of human mesenchymal stem cells to vascular smooth muscle cells.
abstract: In recent years, various approaches have been taken to generate functional muscle tissue by tissue engineering. However, in vitro methods to generate smooth muscle with physiologically aligned structure remains limited. In order to mimic the in vivo highly organized structure of smooth muscle cells, we used micropatterning technology for engineering parallel aligned cells. In this study, a gradient micropattern of different width of cell-adhesive polystyrene stripes (5, 10, 20, 40, 60, 80, 100, 200, 400, 600, 800 and 1000μm) was prepared and the effects of micropattern width on human mesenchymal stem cells (hMSCs) orientation, morphology and smooth muscle cell differentiation were investigated. The width of micropattern stripes showed obvious effect on cell orientation, morphology and smooth muscle cell differentiation. The cells showed higher degree of orientation when the micropattern stripes became narrower. Higher expression of calponin and smooth muscle actin was observed among the narrow micropatterns ranging from 200μm to 20μm, compared to the non-patterned area and wide micropattern areas which showed similar levels of expression.
authors: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. Electronic address: NAKAMOTO.Tomoko@nims.go.jp.
authors: Tomoko
authors: T
authors: Nakamoto
authors: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan; Department of Materials Science and Engineering, Graduate School of Pure and Applied Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571, Japan. Electronic address: WANG.Xinlong@nims.go.jp.
authors: Xinlong
authors: X
authors: Wang
authors: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. Electronic address: KAWAZOE.Naoki@nims.go.jp.
authors: Naoki
authors: N
authors: Kawazoe
authors: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan; Department of Materials Science and Engineering, Graduate School of Pure and Applied Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571, Japan. Electronic address: Guoping.Chen@nims.go.jp.
authors: Guoping
authors: G
authors: Chen
mesh_terms: D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018625:Microscopy, Atomic Force; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D013499:Surface Properties
keywords: Cell orientation; Differentiation; Gradient micropattern; Mesenchymal stem cells; Micropattern width; Vascular smooth muscle cells