title: Application of enzyme/zeolite sensor for urea analysis in serum.
abstract: Urea biosensor based on zeolite-adsorbed urease was applied for analysis of blood serum samples. It should be noted, that this biosensor has a number of advantages, such as simple and fast performance, the absence of toxic compounds during biosensor preparation, high reproducibility and repeatability (RSD=9% and 4%, respectively). The linear range of urea determination by using the biosensor was 0.003-0.75 mM, and the limit of urea detection was 3 μM. The method of standard addition was used for analysis of serum samples with 500-fold dilution. Total time of analysis was 10 min. Good reproducibility of urea determination in real samples was demonstrated (RSD=10%). Biosensor results were verified by using a common method of urea determination (diacetyl monoxime reaction). It was shown that by using this biosensor distinguishing healthy people from people with renal dysfunction becomes easier.
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03680 Kyiv, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska St., 01003 Kyiv, Ukraine.
authors: O O
authors: OO
authors: Soldatkin
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03680 Kyiv, Ukraine; Institute of Biology, Taras Shevchenko National University of Kyiv, 64 Volodymyrska St., 01003 Kyiv, Ukraine. Electronic address: kucherenko.i.s@gmail.com.
authors: I S
authors: IS
authors: Kucherenko
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03680 Kyiv, Ukraine.
authors: S V
authors: SV
authors: Marchenko
authors: Micro and Nanotechnology Department, Middle East Technical University, Ankara 06531, Turkey.
authors: B
authors: B
authors: Ozansoy Kasap
authors: Micro and Nanotechnology Department, Middle East Technical University, Ankara 06531, Turkey; Central Laboratory, Middle East Technical University, Ankara 06531, Turkey.
authors: B
authors: B
authors: Akata
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03680 Kyiv, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska St., 01003 Kyiv, Ukraine.
authors: A P
authors: AP
authors: Soldatkin
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03680 Kyiv, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska St., 01003 Kyiv, Ukraine.
authors: S V
authors: SV
authors: Dzyadevych
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D014508:Urea / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D014510:Urease / Q000378:metabolism*; D017641:Zeolites / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D017641:Zeolites; D014508:Urea; D014510:Urease
keywords: Biosensor; Conductometric transducer; Enzyme adsorption; Silicalite; Urease