title: Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis).
abstract: Little is known about the immune system of cephalopods, in spite of their many highly derived characters within the molluscan clade, including a vertebrate-like high-pressure closed circulatory system. Further the economic importance of cephalopod fisheries, potential for aquaculture, and use as ecotoxicology models demand a thorough understanding of their immune system. In this study, we present a comprehensive characterization of hemocytes in the common cuttlefish Sepia officinalis. Cytological stainings, electron microscopy- and flow cytometry-observations highlight a single granulocyte population with various densities of eosinophilic granules and unstained vesicles. These hemocytes contain acid phosphatase-, lysozyme- and proPO system enzymes, and have high activity in bead phagocytosis assays. Interestingly, bead pre-incubation in plasma results in time-dependent aggregation perhaps resulting from hemocyanin-coating, and decrease in phagocytosis. This study provides the basis for understanding hemocyte-mediated immunity in the common cuttlefish, and essential background for future studies on cephalopod immunity.
authors: Normandie Université, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen Cedex, France; Centre de Recherches en Environnement Côtier, Université de Caen Basse-Normandie, 54 rue du Docteur Charcot, 14530 Luc-sur-Mer, France. Electronic address: charles.lepabic@gmx.fr.
authors: Charles
authors: C
authors: Le Pabic
authors: Normandie Université, F-14032 Caen, France; CMAbio, Université de Caen Basse-Normandie, F-14032 Caen Cedex, France.
authors: Didier
authors: D
authors: Goux
authors: Normandie Université, F-14032 Caen, France; Plateau de cytométrie SFR ICORE, Université de Caen Basse-Normandie, F-14032 Caen Cedex, France.
authors: Maryline
authors: M
authors: Guillamin
authors: Normandie Université, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen Cedex, France; Centre de Recherches en Environnement Côtier, Université de Caen Basse-Normandie, 54 rue du Docteur Charcot, 14530 Luc-sur-Mer, France.
authors: Georges
authors: G
authors: Safi
authors: Normandie Université, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen Cedex, France; Centre de Recherches en Environnement Côtier, Université de Caen Basse-Normandie, 54 rue du Docteur Charcot, 14530 Luc-sur-Mer, France.
authors: Jean-Marc
authors: JM
authors: Lebel
authors: Normandie Université, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen Cedex, France; Centre de Recherches en Environnement Côtier, Université de Caen Basse-Normandie, 54 rue du Docteur Charcot, 14530 Luc-sur-Mer, France.
authors: Noussithé
authors: N
authors: Koueta
authors: Normandie Université, F-14032 Caen, France; UMR BOREA, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen Cedex, France; Centre de Recherches en Environnement Côtier, Université de Caen Basse-Normandie, 54 rue du Docteur Charcot, 14530 Luc-sur-Mer, France.
authors: Antoine
authors: A
authors: Serpentini
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D006434:Hemocytes / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010587:Phagocytosis; D049833:Sepia / Q000276:immunology*
keywords: Cephalopod mollusks; Cuttlefish (Sepia officinalis); Enzymatic assay; Hemocyte population; Phagocytosis