title: Impact of differently modified nanocrystalline diamond on the growth of neuroblastoma cells.
abstract: The aim of this study was to assess the impact of nanocrystalline diamond (NCD) thin coatings on neural cell adhesion and proliferation. NCD was fabricated on fused silica substrates by microwave plasma chemical vapor deposition (MPCVD) method. Different surface terminations were performed through exposure to reactive hydrogen and by UV induced oxidation during ozone treatment. Boron doped NCD coatings were also prepared and investigated. NCD surface wettability was determined by contact angle measurement. To assess biocompatibility of the NCD coatings, the neuroblastoma SH-SY5Y cell line was used. Cells were plated directly onto diamond surfaces and cultured in medium with or without fetal bovine serum (FBS), in order to evaluate the ability of cells to adhere and to proliferate. The obtained results showed that these cells adhered and proliferated better on NCD surfaces than on the bare fused silica. The cell proliferation on NCD in medium with and without FBS after 48h from plating was on average, respectively, 20 and 58% higher than that on fused silica, irrespective of NCD surface modification. Our results showed that the hydrogenated, oxygenated and boron-doped NCD coatings can be used for biomedical purposes, especially where good optical transparency is required.
authors: Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania; Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Zygimantu 9, LT-01102 Vilnius, Lithuania.
authors: Aida
authors: A
authors: Vaitkuviene
authors: Hasselt University, Institute for Materials Research, Division IMOMEC, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors: Matthew
authors: M
authors: McDonald
authors: Hasselt University, Institute for Materials Research, Division IMOMEC, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors: Farnoosh
authors: F
authors: Vahidpour
authors: Hasselt University, Biomedical Research Institute, Agoralaan Building C, Diepenbeek B-3590, Belgium.
authors: Jean-Paul
authors: JP
authors: Noben
authors: Hasselt University, Biomedical Research Institute, Agoralaan Building C, Diepenbeek B-3590, Belgium.
authors: Kathleen
authors: K
authors: Sanen
authors: Hasselt University, Biomedical Research Institute, Agoralaan Building C, Diepenbeek B-3590, Belgium.
authors: Marcel
authors: M
authors: Ameloot
authors: Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania.
authors: Vilma
authors: V
authors: Ratautaite
authors: Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Zygimantu 9, LT-01102 Vilnius, Lithuania; Laboratory of Biomedical Physics, Institute of Oncology, Vilnius University, Santariskiu 1, LT-08660 Vilnius, Lithuania.
authors: Vytautas
authors: V
authors: Kaseta
authors: Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Zygimantu 9, LT-01102 Vilnius, Lithuania.
authors: Gene
authors: G
authors: Biziuleviciene
authors: NanoTechnas-Center of Nanotechnology and Material Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania.
authors: Almira
authors: A
authors: Ramanaviciene
authors: Hasselt University, Institute for Materials Research, Division IMOMEC, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
authors: Milos
authors: M
authors: Nesladek
authors: Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania; Laboratory of NanoBioTechnology, Department of Materials Science and Electronics, Institute of Semiconductor Physics, State Scientific Research Institute Centre for Physical Sciences and Technology, Vilnius, Lithuania. Electronic address: Arunas.Ramanavicius@chf.vu.lt.
authors: Arunas
authors: A
authors: Ramanavicius
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D018130:Diamond / Q000494:pharmacology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry*; D009447:Neuroblastoma / Q000473:pathology*
chemical_list: D020099:Coated Materials, Biocompatible; D018130:Diamond