title: Silencing of phosphoinositide-specific phospholipase C ε remodulates the expression of the phosphoinositide signal transduction pathway in human osteosarcoma cell lines.
abstract: BACKGROUND
  Ezrin, a member of the ezrin-radixin-moesin family, is involved in the metastatic spread of osteosarcoma. Ezrin binds phosphatydil inositol-4,5-bisphosphate (PIP2), a crucial molecule of the phosphoinositide signal transduction pathway. PIP2 levels are regulated by phosphoinositide-specific phospholipase C (PI-PLC) enzymes. PI-PLCε isoform, a well-characterized direct effector of rat sarcoma (RAS), is at a unique convergence point for the broad range of signaling pathways that promote RAS GTPase-mediated signalling.
  
  
  METHODS
  By using molecular biology methods and microscopic analyses, we analyzed the expression of ezrin and PLC genes after silencing of PLCE (OMIM *608414) in 143B and Hs888 cell lines.
  
  
  RESULTS
  The growth rate of the cells was slowed, and the expression of ezrin, PLCB1, PLCG2 and PLCD4 was significantly modified. Ezrin displacement from the plasma membrane was observed.
  
  
  CONCLUSIONS
  The present results corroborate the hypothesis that ezrin and the PI signal transduction system are involved in a common network.
authors: Department of Sense Organs, Sapienza University, Rome, Italy ritalovasco@hotmail.it.
authors: Vincenza Rita
authors: VR
authors: Lo Vasco
authors: Department of Medicine and Surgery Sciences and Biotechnologies, Sapienza University, Rome, Italy.
authors: Martina
authors: M
authors: Leopizzi
authors: Department of Biochemistry Sciences "A. Rossi Fanelli", Sapienza University, Rome, Italy.
authors: Daniela
authors: D
authors: Stoppoloni
authors: Department of Medicine and Surgery Sciences and Biotechnologies, Sapienza University, Rome, Italy.
authors: Carlo
authors: C
authors: Della Rocca
mesh_terms: D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D010716:Phosphatidylinositols / Q000502:physiology*; D054801:Phosphoinositide Phospholipase C / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D054799:Phospholipase C beta / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D010716:Phosphatidylinositols; D054801:Phosphoinositide Phospholipase C; D054799:Phospholipase C beta; D000099149:Ezrin; C518033:PLCB1 protein, human; C423320:phospholipase C epsilon
keywords: Osteosarcoma; ezrin; gene silencing; phosphoinositides; phospholipase c; plce; signal transduction