title: Protective effect of Nigella sativa oil against binge ethanol-induced oxidative stress and liver injury in rats.
abstract: OBJECTIVE
  Nigella sativa L. (Ranunculaceae) is considered as a therapeutic plant-based medicine for liver damage. In this study, the aim was to study the effect of Nigella sativa oil (NSO) pretreatment on ethanol-induced hepatotoxicity in rats.
  
  
  METHODS
  Rats were given Nigella sativa oil at doses of 2.5 and 5.0 mL·kg(-1), orally for 3 weeks, followed by oral ethanol (EtOH) administration (5 g·kg(-1)) every 12 h three times (binge model).
  
  
  RESULTS
  Binge ethanol application caused significant increases in plasma transaminase activities and hepatic triglyceride and malondialdehyde (MDA) levels. It decreased hepatic glutathione (GSH) levels, but did not change vitamins E and vitamin C levels and antioxidant enzyme activities. NSO (5.0 mL·kg(-1)) pretreatment significantly decreased plasma transaminase activities, hepatic MDA, and triglyceride levels together with amelioration in hepatic histopathological findings.
  
  
  CONCLUSIONS
  NSO pretreatment may be effective in protecting oxidative stress-induced hepatotoxicity after ethanol administration.
authors: Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, 34093, Istanbul, Turkey.
authors: Seval
authors: S
authors: Develi
authors: Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, 34093, Istanbul, Turkey.
authors: Betül
authors: B
authors: Evran
authors: Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, 34093, Istanbul, Turkey.
authors: Esra
authors: E
authors: Betül Kalaz
authors: Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, 34093, Istanbul, Turkey.
authors: Necla
authors: N
authors: Koçak-Toker
authors: Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, 34093, Istanbul, Turkey. Electronic address: gul_erata@yahoo.com.
authors: Gül Özdemirler
authors: GÖ
authors: Erata
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D008108:Liver Diseases, Alcoholic / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D031881:Nigella sativa / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010938:Plant Oils / Q000008:administration & dosage*; D020011:Protective Agents / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D000637:Transaminases / Q000097:blood; D029974:Carum
chemical_list: D010938:Plant Oils; D020011:Protective Agents; D000431:Ethanol; D008315:Malondialdehyde; C104525:caraway oil; D013482:Superoxide Dismutase; D000637:Transaminases
keywords: Binge ethanol; Liver; Nigella sativa; Oxidative stress; Rats