title: Bio-Oss(®) for delayed osseointegration of implants in dogs: a histological study.
abstract: We evaluated the effects of Bio-Oss® (a natural bone substitute derived from the mineral portion of bovine bone) on delayed osseointegration of implants. The bilateral third and fourth mandibular premolars of 4 adult, healthy, male and female dogs were extracted. We randomly selected 2 extraction sockets in each dog to be filled with Bio-Oss® (the experimental group); the other 2 extraction sockets, which were not treated, served as controls. Dental implants were inserted into the alveolar bone of the experimental group and the control group 3 months after insertion of the Bio-Oss®. The osteogenic activity in the bone around the implants was assessed by evaluating the histological morphology and estimating histomorphometric variables at 3 and 6 months after delayed implantation. After 3 months, Goldner's trichrome staining analysis showed that the rate of content between the bone and the implant and the mineralised area of bone around the implant were significantly higher in the experimental group (76%(9%) and 69.5% (9.6%), respectively) than those in the control group (56.1% (8.2%) and 52.8% (7.3%), respectively, p=0.003 and 0.000). However, the 2 groups did not differ significantly at 6 months. Fluorescence microscopy showed that the mean rates of mineralisation of the bony tissue around the implant in the experimental group at months 3 and 6 were 6.8 (0.4) μm and 8.4 (0.8) μm, respectively, which were significantly higher than those in the control group (p=0.000 and 0.03). These data indicate that putting Bio-Oss® into the extraction sockets can promote osseointegration after delayed implantation, and may be a promising option for clinical use.
authors: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian, 116044, PR China.
authors: Hui-ying
authors: HY
authors: Liu
authors: Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Dalian, 116044, PR China; Department of Oral Pathology, Dental Science Research Institute, Chonnam National University, Bug-Gu, Gwangju, 500757, Korea.
authors: Hui
authors: H
authors: Zheng
authors: Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Dalian, 116044, PR China.
authors: Xi-peng
authors: XP
authors: Hou
authors: Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Dalian, 116044, PR China.
authors: Wei-jian
authors: WJ
authors: Zhong
authors: Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian, 116044, PR China.
authors: Xiao-xia
authors: XX
authors: Ying
authors: Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Dalian, 116044, PR China.
authors: Song-ling
authors: SL
authors: Chai
authors: Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Dalian, 116044, PR China. Electronic address: mgw64024@163.com.
authors: Guo-wu
authors: GW
authors: Ma
mesh_terms: D000540:Alveolar Ridge Augmentation / Q000379:methods; D000818:Animals; D001391:Azo Compounds; D015519:Bone Density / Q000502:physiology; D001857:Bone Matrix / Q000473:pathology; D018786:Bone Substitutes / Q000627:therapeutic use*; D002113:Calcification, Physiologic / Q000502:physiology; D004396:Coloring Agents; D003758:Dental Implantation, Endosseous / Q000379:methods; D015921:Dental Implants; D004285:Dogs; D004801:Eosine Yellowish-(YS); D005260:Female; D006253:Haversian System / Q000473:pathology; D008297:Male; D008334:Mandible / Q000473:pathology / Q000601:surgery; D008739:Methyl Green; D008856:Microscopy, Fluorescence; D008903:Minerals / Q000627:therapeutic use*; D016348:Osseointegration / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D011897:Random Allocation; D013499:Surface Properties; D013997:Time Factors; D020390:Tooth Socket / Q000473:pathology / Q000601:surgery
chemical_list: D001391:Azo Compounds; C077540:Bio-Oss; D018786:Bone Substitutes; D004396:Coloring Agents; D015921:Dental Implants; D008903:Minerals; C017768:trichrome stain; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)
keywords: Bio-Oss®; delayed implantation; implant; osseointegration