title: Porous hydrogel of wool keratin prepared by a novel method: an extraction with guanidine/2-mercaptoethanol solution followed by a dialysis.
abstract: In this study, we show a novel simple method to prepare a sponge-like porous keratin hydrogel through the extraction of wool keratin in a solution containing guanidine hydrochloride and 2-mercaptoethanol followed by dialysis for both aggregation of keratin and recrosslink. The gel had a highly porous structure and a fast-swelling property in rehydration after freeze-drying. It had also high mechanical strength both in the tensile test and the measurement of dynamic viscoelasticity. Three types of animal cells, PC12 cells, HOS cells and murine embryonic fibroblasts, well attached and grew on the surface of the porous hydrogel.
authors: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors: Yuki
authors: Y
authors: Ozaki
authors: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors: Yusuke
authors: Y
authors: Takagi
authors: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors: Hideki
authors: H
authors: Mori
authors: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan. Electronic address: hara@b.s.osakafu-u.ac.jp.
authors: Masayuki
authors: M
authors: Hara
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000302:isolation & purification*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation; D002460:Cell Line; D002470:Cell Survival; D003956:Dialysis; D019791:Guanidine; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007633:Keratins / Q000737:chemistry* / Q000302:isolation & purification*; D008623:Mercaptoethanol; D051379:Mice; D016716:PC12 Cells; D016062:Porosity; D051381:Rats; D012756:Sheep; D054457:Tissue Scaffolds / Q000737:chemistry; D014935:Wool / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008623:Mercaptoethanol; D007633:Keratins; D019791:Guanidine
keywords: Guanidine; Hydrogel; Keratin; Sponge; Wool