title: Semaphorin 3F and neuropilin-2 control the migration of human T-cell precursors.
abstract: Neuropilins and semaphorins are known as modulators of axon guidance, angiogenesis, and organogenesis in the developing nervous system, but have been recently evidenced as also playing a role in the immune system. Here we describe the expression and role of semaphorin 3F (SEMA3F) and its receptor neuropilin-2 (NRP2) in human T cell precursors. NRP2 and SEMA3F are expressed in the human thymus, in both lymphoid and non-lymphoid compartments. SEMA3F have a repulsive effect on thymocyte migration and inhibited CXCL12- and sphingosine-1-phosphate (S1P)-induced thymocyte migration by inhibiting cytoskeleton reorganization prior to stimuli. Moreover, NRP2 and SEMA3F are expressed in human T-cell acute lymphoblastic leukemia/lymphoma primary cells. In these tumor cells, SEMA3F also blocks their migration induced by CXCL12 and S1P. Our data show that SEMA3F and NRP2 are further regulators of human thymocyte migration in physiological and pathological conditions.
authors: CNRS UMR8147, Paris Descartes University, Paris, France; Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors: Daniella Arêas
authors: DA
authors: Mendes-da-Cruz
authors: CNRS UMR8147, Paris Descartes University, Paris, France; Department of Clinical Hematology, Necker Children's Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France.
authors: Anne Colette
authors: AC
authors: Brignier
authors: Laboratory of Oncohematology, AP-HP, Necker Children's Hospital, INSERM U1151, Paris, France.
authors: Vahid
authors: V
authors: Asnafi
authors: Laboratory of Oncohematology, AP-HP, Necker Children's Hospital, INSERM U1151, Paris, France.
authors: Frederic
authors: F
authors: Baleydier
authors: Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors: Carolina Valença
authors: CV
authors: Messias
authors: CNRS UMR8147, Paris Descartes University, Paris, France; INSERM U1163, CNRS ERL 8254, Laboratory of cellular and molecular basis of hematological disorders and their therapeutic implications, Imagine Institute, Paris, France.
authors: Yves
authors: Y
authors: Lepelletier
authors: Laboratory of Oncohematology, AP-HP, Necker Children's Hospital, INSERM U1151, Paris, France.
authors: Nawel
authors: N
authors: Bedjaoui
authors: CNRS UMR8147, Paris Descartes University, Paris, France.
authors: Amedée
authors: A
authors: Renand
authors: Department of Morphology, Federal University of Alagoas, Maceió, Brazil.
authors: Salete
authors: S
authors: Smaniotto
authors: Laboratory of Oncohematology, AP-HP, Necker Children's Hospital, INSERM U1151, Paris, France; Department of Morphology, Federal University of Alagoas, Maceió, Brazil.
authors: Danielle
authors: D
authors: Canioni
authors: CNRS UMR8147, Paris Descartes University, Paris, France.
authors: Pierre
authors: P
authors: Milpied
authors: INSERM U819 - Pasteur Institute, Paris, France.
authors: Karl
authors: K
authors: Balabanian
authors: INSERM U668 - Pasteur Institute, Paris, France.
authors: Philippe
authors: P
authors: Bousso
authors: Department of Hematology, Centre Henri Becquerel, Rouen, France.
authors: Stéphane
authors: S
authors: Leprêtre
authors: Service of Pediatric Hematology, Hôpital Debrousse, Lyon, France.
authors: Yves
authors: Y
authors: Bertrand
authors: University Paris 7, Hôpital Saint-Louis, AP-HP, and Institut Universitaire d'Hématologie EA3518, Paris, France.
authors: Hervé
authors: H
authors: Dombret
authors: Pôle de Recherche et d'Enseignement Supérieur l'Université Nantes Angers Le Mans, Centre Hospitalier Universitaire Angers, Service des Maladies du Sang and INSERM U892, Angers, France.
authors: Norbert
authors: N
authors: Ifrah
authors: CNRS UMR8147, Paris Descartes University, Paris, France.
authors: Mireille
authors: M
authors: Dardenne
authors: Laboratory of Oncohematology, AP-HP, Necker Children's Hospital, INSERM U1151, Paris, France.
authors: Elizabeth
authors: E
authors: Macintyre
authors: Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors: Wilson
authors: W
authors: Savino
authors: CNRS UMR8147, Paris Descartes University, Paris, France; Laboratory of Oncohematology, AP-HP, Necker Children's Hospital, INSERM U1151, Paris, France; INSERM U1163, CNRS ERL 8254, Laboratory of cellular and molecular basis of hematological disorders and their therapeutic implications, Imagine Institute, Paris, France.
authors: Olivier
authors: O
authors: Hermine
mesh_terms: D019138:Antibodies, Blocking / Q000276:immunology / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D015870:Gene Expression; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008246:Lysophospholipids / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D039943:Neuropilin-2 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D054504:Precursor Cells, T-Lymphoid / Q000378:metabolism*; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013110:Sphingosine / Q000031:analogs & derivatives / Q000494:pharmacology; D060168:Thymocytes / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism
chemical_list: D019138:Antibodies, Blocking; D054377:Chemokine CXCL12; D008246:Lysophospholipids; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D039943:Neuropilin-2; C491230:SEMA3F protein, human; C581308:neuropilin-2, human; C060506:sphingosine 1-phosphate; D013110:Sphingosine