title: Multiresidue determination of ten nonsteroidal anti-inflammatory drugs in bovine, porcine, and chicken liver tissues by HPLC-MS/MS.
abstract: Nonsteroidal anti-inflammatory drugs (NSAIDs) are the group of drugs having the therapeutic efficacy of analgesic and antipyretic. To detect health-threatening residues of NSAIDs, a fast and easy multiresidue method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) was described. Ten NSAIDs were extracted from the tissues using 2 mL of acetonitrile and 0.1 mL of 2 mM ammonium formate in distilled water. After clean-up using C18 sorbent, it was evaporated under nitrogen, reconstituted with 1 mL distilled water and analyzed by LC-MS/MS. The method was validated based on guideline for residue testing laboratory. Furthermore, the method has also been applied successfully to detect ten NSAIDs from bovine, porcine, and chicken liver tissues. In a total of 315 liver samples tested, acetylic salicylic acid was detected from 28 porcine and 2 chicken liver tissues at levels of 13 ∼ 576 and 50 ∼ 53 ng/g, respectively. Subsequently, paracetamol was detected in 15 porcine liver tissues with a detection levels of 28 ∼ 381 ng/g. Phenylbutazone and its metabolite, oxyphenylbutazone, were detected at 247 and 15 ng/g range in one of the bovine liver tissue, respectively.
authors: Veterinary drug and Biologics Division, Animal and Plant Quarantine and Inspection Agency, Gyeonggi-do, 430-757, Republic of Korea.
authors: JeongWoo
authors: J
authors: Kang
authors: Su-Jeong
authors: SJ
authors: Park
authors: Hae-Chul
authors: HC
authors: Park
authors: Vinayakumar
authors: V
authors: Gedi
authors: ByungJae
authors: B
authors: So
authors: Kwang-Jick
authors: KJ
authors: Lee
mesh_terms: D000082:Acetaminophen / Q000493:pharmacokinetics* / Q000494:pharmacology; D018712:Analgesics, Non-Narcotic / Q000493:pharmacokinetics* / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000493:pharmacokinetics* / Q000494:pharmacology; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods; D008099:Liver / Q000378:metabolism*; D010653:Phenylbutazone / Q000493:pharmacokinetics* / Q000494:pharmacology; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D018712:Analgesics, Non-Narcotic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000082:Acetaminophen; D010653:Phenylbutazone