title: Development and characterization of a novel, antimicrobial, sterile hydrogel dressing for burn wounds: single-step production with gamma irradiation creates silver nanoparticles and radical polymerization.
abstract: Patients with burn wounds are susceptible to wound infection and sepsis. This research introduces a novel burn wound dressing that contains silver nanoparticles (SNPs) to treat infection in a 2-acrylamido-2-methylpropane sulfonic acid sodium salt (AMPS-Na(+) ) hydrogel. Silver nitrate was dissolved in AMPS-Na(+) solution and then exposed to gamma irradiation to form SNP-infused hydrogels. The gamma irradiation results in a cross-linked polymeric network of sterile hydrogel dressing and a reduction of silver ions to form SNPs infused in the hydrogel in a one-step process. About 80% of the total silver was released from the hydrogels after 72 h immersion in simulated body fluid solution; therefore, they could be used on wounds for up to 3 days. All the hydrogels were found to be nontoxic to normal human dermal fibroblast cells. The silver-loaded hydrogels had good inhibitory action against Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus. Results from a pilot study on a porcine burn model showed that the 5-mM silver hydrogel was efficient at preventing bacterial colonization of wounds, and the results were comparable to the commercially available silver dressings (Acticoat(TM) , PolyMem Silver(®) ). These results support its use as a potential burn wound dressing.
authors: Department of Chemical and Biomolecular Engineering, Tulane University, New Orleans, Louisiana, 70118.
authors: Benjawan
authors: B
authors: Boonkaew
authors: Philip M
authors: PM
authors: Barber
authors: Sirirat
authors: S
authors: Rengpipat
authors: Pitt
authors: P
authors: Supaphol
authors: Margit
authors: M
authors: Kempf
authors: Jibao
authors: J
authors: He
authors: Vijay T
authors: VT
authors: John
authors: Leila
authors: L
authors: Cuttle
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001458:Bandages; D005720:Gamma Rays; D020100:Hydrogels; D053768:Metal Nanoparticles; D058105:Polymerization; D012834:Silver / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D012834:Silver
keywords: antimicrobial activity; burn wounds; cell culture; dressings; gamma irradiation; hydrogels; nanoparticles; polymer synthesis; silver; spectroscopy