title: An efficient DNA-fueled molecular machine for the discrimination of single-base changes.
abstract: A new strategy for single-base polymorphism (SNP) detection based on the assembly of DNA-AuNPs (gold nanoparticles) driven by a DNA-fueled molecular machine, is established and optimized. It is highly efficient, works at room temperature, and is easy to handle. A single-base change on an oligonucleotide strand is unambiguously discriminated for either SNPs or insertions and deletions (indels). The strategy is demonstrated to detect a mutation in the breast cancer gene BRCA1 in homogeneous solution at room temperature.
authors: CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, PR China.
authors: Tingjie
authors: T
authors: Song
authors: Shiyan
authors: S
authors: Xiao
authors: Dongbao
authors: D
authors: Yao
authors: Fujian
authors: F
authors: Huang
authors: Maobin
authors: M
authors: Hu
authors: Haojun
authors: H
authors: Liang
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D005260:Female; D005838:Genotype; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013056:Spectrophotometry, Ultraviolet
chemical_list: D019313:BRCA1 Protein; D006046:Gold; D004247:DNA
keywords: DNA assembly; DNA molecular machine; gold nanoparticles; single-base change; single-base polymorphisms (SNPs)