title: Facile synthesis of multifunctional multi-walled carbon nanotube for pathogen Vibrio alginolyticus detection in fishery and environmental samples.
abstract: Interest in carbon nanotubes for detecting the presence of pathogens arises because of developments in chemical vapor deposition synthesis and progresses in biomolecular modification. Here we reported the facile synthesis of multi-walled carbon nanotubes (MWCNTs), which functioned as immuno-, magnetic, fluorescent sensors in detecting Vibrio alginolyticus (Va). The structures and properties of functionalized MWCNTs were characterized by ultraviolet (UV), Fourier transform infrared spectra (FT-IR), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), magnetic property measurement system (MPMS) and fluorescent spectra (FL). It was found that the functionalized MWCNTs showed: (1) low nonspecific adsorption for antibody-antigen, (2) strong interaction with antibody, and (3) high immune-magnetic activity for pathogenic cells. Further investigations revealed a strong positive linear relationship (R=0.9912) between the fluorescence intensity and the concentration of Va in the range of 9.0 × 10(2) to 1.5 × 10(6) cfum L(-1). Moreover, the relative standard deviation for 11 replicate detections of 1.0 × 10(4) cfum L(-1) Va was 2.4%, and no cross-reaction with the other four strains was found, indicating a good specificity for Va detection. These results demonstrated the remarkable advantages of the multifunctional MWCNTs, which offer great potential for the rapid, sensitive and quantitative detection of Va in fishery and environmental samples.
authors: Tianjin Key Laboratory of Structure and Performance for Functional Molecules, Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry, Ministry of Education, College of Chemistry, Tianjin Normal University, Tianjin 300387, China. Electronic address: nkcmliu@yahoo.com.
authors: Yue
authors: Y
authors: Liu
authors: Beijing Guodian Futong Science and Technology Development Co., Ltd., Beijing 102401, China.
authors: Jia
authors: J
authors: Hu
authors: Tianjin Center for Control and Prevention of Aquatic Animal Infectious Disease, Tianjin Fishery Institute, Tianjin 300221, China.
authors: Jin-Sheng
authors: JS
authors: Sun
authors: Department of Chemistry, Nankai University, Tianjin 300071, China.
authors: Yan
authors: Y
authors: Li
authors: Tianjin Center for Control and Prevention of Aquatic Animal Infectious Disease, Tianjin Fishery Institute, Tianjin 300221, China.
authors: Shu-Xia
authors: SX
authors: Xue
authors: Tianjin Guangfu Fine Chemical Research Institute, Tianjin 300384, China.
authors: Xiao-Qin
authors: XQ
authors: Chen
authors: Tianjin Key Laboratory of Structure and Performance for Functional Molecules, Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry, Ministry of Education, College of Chemistry, Tianjin Normal University, Tianjin 300387, China.
authors: Xiao-Shuang
authors: XS
authors: Li
authors: Tianjin Key Laboratory of Structure and Performance for Functional Molecules, Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry, Ministry of Education, College of Chemistry, Tianjin Normal University, Tianjin 300387, China.
authors: Gui-Xiang
authors: GX
authors: Du
mesh_terms: D000818:Animals; D004784:Environmental Monitoring / Q000379:methods; D005396:Fish Products / Q000382:microbiology*; D005398:Fisheries; D005516:Food Microbiology / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry*; D033561:Penaeidae / Q000382:microbiology*; D061005:Ponds / Q000382:microbiology*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D044149:Vibrio alginolyticus / Q000737:chemistry / Q000302:isolation & purification*; D014871:Water Microbiology; D014961:X-Ray Diffraction
chemical_list: D037742:Nanotubes, Carbon
keywords: Immuno-magnetic-fluorescent sensor; Multi-walled carbon nanotubes; Ultrasensitive detection; Vibrio alginolyticus (Va)