title: Selective detection of endogenous H₂S in living cells and the mouse hippocampus using a ratiometric fluorescent probe.
abstract: As one of three gasotransmitters, the fundamental signalling roles of hydrogen sulphide are receiving increasing attention. New tools for the accurate detection of hydrogen sulphide in cells and tissues are in demand to probe its biological functions. We report the p-nitrobenzyl-based ratiometric fluorescent probe RHP-2, which features a low detection limit, high selectivity and good photostability. The emission intensity ratios had a good linear relationship with the sulphide concentrations in PBS buffer and bovine serum. Our probe was applied to the ratiometric determination and imaging of endogenous H2S in living cells. Furthermore, RHP-2 was used as an effective tool to measure endogenous H2S in the mouse hippocampus. We observed a significant reduction in sulphide concentrations and downregulated expression of cystathionine β-synthetase (CBS) mRNA and CBS protein in the mouse hippocampus in a chronic unpredictable mild stress (CUMS)-induced depression model. These data suggested that decreased concentrations of endogenous H2S may be involved in the pathogenesis of chronic stress depression.
authors: 1] State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Institute of Chemistry and BioMedical Sciences, Nanjing University, Nanjing, 210093, China [2] School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China [3].
authors: Ling
authors: L
authors: Zhang
authors: 1] School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China [2].
authors: Wen-qi
authors: WQ
authors: Meng
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Institute of Chemistry and BioMedical Sciences, Nanjing University, Nanjing, 210093, China.
authors: Liang
authors: L
authors: Lu
authors: School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China.
authors: Yun-Sheng
authors: YS
authors: Xue
authors: School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China.
authors: Cheng
authors: C
authors: Li
authors: School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China.
authors: Fang
authors: F
authors: Zou
authors: School of Pharmacy, Xuzhou Medical College, Xuzhou, 221002, China.
authors: Yi
authors: Y
authors: Liu
authors: 1] State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Institute of Chemistry and BioMedical Sciences, Nanjing University, Nanjing, 210093, China [2] Guangdong Key Lab of Nano-Micro Material Research, School of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University, Shenzhen, 518055, China.
authors: Jing
authors: J
authors: Zhao
mesh_terms: D000818:Animals; D002219:Carbamates / Q000138:chemical synthesis*; D002417:Cattle; D003541:Cystathionine beta-Synthase / Q000235:genetics / Q000378:metabolism; D003863:Depression / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D004195:Disease Models, Animal; D015536:Down-Regulation; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000378:metabolism; D057230:Limit of Detection; D061986:MCF-7 Cells; D051379:Mice; D015335:Molecular Probes / Q000138:chemical synthesis*; D053644:Naphthalimides / Q000138:chemical synthesis*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence / Q000379:methods; D013315:Stress, Psychological / Q000150:complications / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D002219:Carbamates; D005456:Fluorescent Dyes; D015335:Molecular Probes; D053644:Naphthalimides; C000602222:RHP-2 compound; D012333:RNA, Messenger; D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide