title: Image segmentation and identification of paired antibodies in breast tissue.
abstract: Comparing staining patterns of paired antibodies designed towards a specific protein but toward different epitopes of the protein provides quality control over the binding and the antibodies' ability to identify the target protein correctly and exclusively. We present a method for automated quantification of immunostaining patterns for antibodies in breast tissue using the Human Protein Atlas database. In such tissue, dark brown dye 3,3'-diaminobenzidine is used as an antibody-specific stain whereas the blue dye hematoxylin is used as a counterstain. The proposed method is based on clustering and relative scaling of features following principal component analysis. Our method is able (1) to accurately segment and identify staining patterns and quantify the amount of staining and (2) to detect paired antibodies by correlating the segmentation results among different cases. Moreover, the method is simple, operating in a low-dimensional feature space, and computationally efficient which makes it suitable for high-throughput processing of tissue microarrays.
authors: Centre for Image Analysis, Department of Information Technology, Uppsala University, 75105 Uppsala, Sweden.
authors: Jimmy C
authors: JC
authors: Azar
authors: Centre for Image Analysis, Department of Information Technology, Uppsala University, 75105 Uppsala, Sweden.
authors: Martin
authors: M
authors: Simonsson
authors: Centre for Image Analysis, Department of Information Technology, Uppsala University, 75105 Uppsala, Sweden.
authors: Ewert
authors: 0000-0002-1636-3469
authors: E
authors: Bengtsson
authors: Centre for Image Analysis, Department of Information Technology, Uppsala University, 75105 Uppsala, Sweden.
authors: Anders
authors: 0000-0003-1054-2754
authors: A
authors: Hast
mesh_terms: D015100:3,3'-Diaminobenzidine / Q000737:chemistry*; D000465:Algorithms; D000906:Antibodies / Q000737:chemistry*; D001940:Breast / Q000473:pathology*; D016000:Cluster Analysis; D030562:Databases, Protein; D005260:Female; D006416:Hematoxylin / Q000737:chemistry; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D016011:Normal Distribution; D025341:Principal Component Analysis; D012984:Software; D013194:Staining and Labeling; D046888:Tissue Array Analysis
chemical_list: D000906:Antibodies; D015100:3,3'-Diaminobenzidine; D006416:Hematoxylin