title: Helicobacter pylori filtrate induces Alzheimer-like tau hyperphosphorylation by activating glycogen synthase kinase-3β.
abstract: Abnormal hyperphosphorylation of microtubule-associated protein tau is involved in the pathogenesis of several neurodegenerative disorders including Alzheimer's disease (AD). Helicobacter pylori (H. pylori) infection has been reported to be related with a high risk of AD, but the direct laboratory evidence is lacking. Here we explored the effect of H. pylori infection on tau phosphorylation. The results showed that H. pylori filtrate induced significant tau hyperphosphorylation at several AD-related tau phosphorylation sites, such as Thr205, Thr231, and Ser404, both in mouse neuroblastoma N2a cells and rat brains with activation of glycogen synthase kinase-3β (GSK-3β). Application of GSK-3 inhibitors efficiently attenuated the H. pylori-induced tau hyperphosphorylation. Our data provide evidence supporting the role of H. pylori infection in AD-like tau pathology, suggesting that H. pylori eradication may be beneficial in the prevention of tauopathy.
authors: Key Laboratory of Neurological Disease, Ministry of Education, China, and Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Department of Pathology, Hubei University of Chinese Medicine, Wuhan, China.
authors: Xiu-Lian
authors: XL
authors: Wang
authors: Department of Clinical Laboratory, Wuhan Pu Ai Hospital, Huazhong University of Science and Technology, Wuhan, China.
authors: Ji
authors: J
authors: Zeng
authors: Key Laboratory of Neurological Disease, Ministry of Education, China, and Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Yang
authors: Y
authors: Yang
authors: Key Laboratory of Neurological Disease, Ministry of Education, China, and Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Yan
authors: Y
authors: Xiong
authors: Department of Pathology, Hubei University of Chinese Medicine, Wuhan, China.
authors: Zhi-Hua
authors: ZH
authors: Zhang
authors: Key Laboratory of Neurological Disease, Ministry of Education, China, and Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Mei
authors: M
authors: Qiu
authors: Key Laboratory of Neurological Disease, Ministry of Education, China, and Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Xiong
authors: X
authors: Yan
authors: Key Laboratory of Neurological Disease, Ministry of Education, China, and Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Xu-Ying
authors: XY
authors: Sun
authors: Key Laboratory of Neurological Disease, Ministry of Education, China, and Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Qing-Zhang
authors: QZ
authors: Tuo
authors: Key Laboratory of Neurological Disease, Ministry of Education, China, and Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Rong
authors: R
authors: Liu
authors: Key Laboratory of Neurological Disease, Ministry of Education, China, and Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Jian-Zhi
authors: JZ
authors: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D016207:Cytokines / Q000378:metabolism; D004926:Escherichia coli; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D016480:Helicobacter pylori; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D017628:Microglia / Q000502:physiology; D010766:Phosphorylation / Q000502:physiology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D016207:Cytokines; C558298:Mapt protein, mouse; C489131:Mapt protein, rat; D047428:Protein Kinase Inhibitors; D016875:tau Proteins; D000071679:Glycogen Synthase Kinase 3 beta; C000605507:Gsk3b protein, mouse; C000605508:Gsk3b protein, rat; D038362:Glycogen Synthase Kinase 3
keywords: Alzheimer's disease; Helicobacter pylori; glycogen synthase kinase-3β; tau hyperphosphorylation