title: Defining suitable reference genes for RT-qPCR analysis on human sertoli cells after 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure.
abstract: Quantitative real-time RT-PCR (qPCR) has proven to be a valuable molecular technique to quantify gene expression. There are few studies in the literature that describe suitable reference genes to normalize gene expression data. Studies of transcriptionally disruptive toxins, like tetrachlorodibenzo-p-dioxin (TCDD), require careful consideration of reference genes. The present study was designed to validate potential reference genes in human Sertoli cells after exposure to TCDD. 32 candidate reference genes were analyzed to determine their applicability. geNorm and NormFinder softwares were used to obtain an estimation of the expression stability of the 32 genes and to identify the most suitable genes for qPCR data normalization.
authors: Department of Morphology, Institute of Biosciences, UNESP- Univ Estadual Paulista, Botucatu, SP, 18618-970, Brazil.
authors: Mariana Antunes
authors: MA
authors: Ribeiro
authors: Mariana Bisarro
authors: MB
authors: dos Reis
authors: Leonardo Nazário
authors: LN
authors: de Moraes
authors: Christine
authors: C
authors: Briton-Jones
authors: Cláudia Aparecida
authors: CA
authors: Rainho
authors: Wellerson Rodrigo
authors: WR
authors: Scarano
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000235:genetics*; D006801:Humans; D008297:Male; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D000072317:Polychlorinated Dibenzodioxins