title: Probing the conformational changes of ovalbumin after glycation using HDX-MS.
abstract: The conformational changes of the glycated ovalbumin were studied by hydrogen/deuterium exchange coupled with high resolution mass spectrometry technique (HDX-MS). After incubation with glucose at 50 °C for 6 h, 9 glycated peptides were detected and the corresponding glycation sites were identified. The glycation extent of each peptide was relatively high, almost over 0.5 in all peptides. A detailed peptide mapping revealed that most of the peptides, including the glycated and non-glycated were protected. The glycation sites not only influence the local region but also the distant area. The enhanced hydrogen protection after glycation suggests that the protein adopts a more stable conformation.
authors: College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
authors: Xiaoqin
authors: X
authors: Huang
authors: College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China. Electronic address: tuzc_mail@aliyun.com.
authors: Zongcai
authors: Z
authors: Tu
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
authors: Hui
authors: H
authors: Wang
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
authors: Qiuting
authors: Q
authors: Zhang
authors: College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.
authors: Yuan
authors: Y
authors: Chen
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
authors: Yan
authors: Y
authors: Shi
authors: Department of Pathology, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY 10461, United States. Electronic address: hui.xiao@einstein.yu.edu.
authors: Hui
authors: H
authors: Xiao
mesh_terms: D003903:Deuterium / Q000737:chemistry*; D006031:Glycosylation; D006859:Hydrogen / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010047:Ovalbumin / Q000737:chemistry*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry
chemical_list: D010455:Peptides; D006859:Hydrogen; D010047:Ovalbumin; D003903:Deuterium
keywords: Conformation; Glycation; Hydrogen/deuterium exchange; Mass spectrometry; Ovalbumin