title: DNA-affinity-purified chip (DAP-chip) method to determine gene targets for bacterial two component regulatory systems.
abstract: In vivo methods such as ChIP-chip are well-established techniques used to determine global gene targets for transcription factors. However, they are of limited use in exploring bacterial two component regulatory systems with uncharacterized activation conditions. Such systems regulate transcription only when activated in the presence of unique signals. Since these signals are often unknown, the in vitro microarray based method described in this video article can be used to determine gene targets and binding sites for response regulators. This DNA-affinity-purified-chip method may be used for any purified regulator in any organism with a sequenced genome. The protocol involves allowing the purified tagged protein to bind to sheared genomic DNA and then affinity purifying the protein-bound DNA, followed by fluorescent labeling of the DNA and hybridization to a custom tiling array. Preceding steps that may be used to optimize the assay for specific regulators are also described. The peaks generated by the array data analysis are used to predict binding site motifs, which are then experimentally validated. The motif predictions can be further used to determine gene targets of orthologous response regulators in closely related species. We demonstrate the applicability of this method by determining the gene targets and binding site motifs and thus predicting the function for a sigma54-dependent response regulator DVU3023 in the environmental bacterium Desulfovibrio vulgaris Hildenborough.
authors: Physical Biosciences Division, Lawrence Berkeley National Laboratory; lrajeev@lbl.gov.
authors: Lara
authors: L
authors: Rajeev
authors: Physical Biosciences Division, Lawrence Berkeley National Laboratory.
authors: Eric G
authors: EG
authors: Luning
authors: Physical Biosciences Division, Lawrence Berkeley National Laboratory.
authors: Aindrila
authors: A
authors: Mukhopadhyay
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D016969:Desulfovibrio vulgaris / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015398:Signal Transduction
chemical_list: D004247:DNA