title: A long non-coding RNA snaR contributes to 5-fluorouracil resistance in human colon cancer cells.
abstract: Several types of genetic and epigenetic regulation have been implicated in the development of drug resistance, one significant challenge for cancer therapy. Although changes in the expression of non-coding RNA are also responsible for drug resistance, the specific identities and roles of them remain to be elucidated. Long non-coding RNAs (lncRNAs) are a type of ncRNA (> 200 nt) that influence the regulation of gene expression in various ways. In this study, we aimed to identify differentially expressed lncRNAs in 5-fluorouracil-resistant colon cancer cells. Using two pairs of 5-FU-resistant cells derived from the human colon cancer cell lines SNU-C4 and SNU-C5, we analyzed the expression of 90 lncRNAs by qPCR-based profiling and found that 19 and 23 lncRNAs were differentially expressed in SNU-C4R and SNU-C5R cells, respectively. We confirmed that snaR and BACE1AS were downregulated in resistant cells. To further investigate the effects of snaR on cell growth, cell viability and cell cycle were analyzed after transfection of siRNAs targeting snaR. Down-regulation of snaR decreased cell death after 5-FU treatment, which indicates that snaR loss decreases in vitro sensitivity to 5-FU. Our results provide an important insight into the involvement of lncRNAs in 5-FU resistance in colon cancer cells.
authors: Department of Biochemistry, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea.
authors: Heejin
authors: H
authors: Lee
authors: Department of Biochemistry, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea.
authors: Chongtae
authors: C
authors: Kim
authors: Cancer Research Institute and Cancer Research Center, Seoul National University, Seoul 110-744, Korea.
authors: Ja-Lok
authors: JL
authors: Ku
authors: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea.
authors: Wook
authors: W
authors: Kim
authors: Cancer Evolution Research Center, Catholic University of Korea, Seoul 137-701, Korea ; Department of Biomedical Science, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea.
authors: Sungjoo Kim
authors: SK
authors: Yoon
authors: Cancer Evolution Research Center, Catholic University of Korea, Seoul 137-701, Korea ; Department of Biomedical Science, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea.
authors: Hyo-Jeong
authors: HJ
authors: Kuh
authors: Department of Biochemistry, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea ; Cancer Evolution Research Center, Catholic University of Korea, Seoul 137-701, Korea.
authors: Jeong-Hwa
authors: JH
authors: Lee
authors: Cancer Evolution Research Center, Catholic University of Korea, Seoul 137-701, Korea ; Department of Pathology, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea.
authors: Suk Woo
authors: SW
authors: Nam
authors: Department of Biochemistry, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea ; Cancer Evolution Research Center, Catholic University of Korea, Seoul 137-701, Korea.
authors: Eun Kyung
authors: EK
authors: Lee
mesh_terms: D002453:Cell Cycle / Q000187:drug effects / Q000276:immunology; D016923:Cell Death / Q000187:drug effects / Q000235:genetics; D048708:Cell Growth Processes / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000235:genetics; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D062085:RNA, Long Noncoding / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D059467:Transcriptome
chemical_list: C561565:BACE1-AS long non-coding RNA, human; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; C000621172:long non-coding RNA snaR, human; D005472:Fluorouracil
keywords: 5-Fluorouracil; cell viability; drug resistance; long non-coding RNAs; snaR