title: Controlled heterogeneous stem cell differentiation on a shape memory hydrogel surface.
abstract: The success of stem cell therapies is highly dependent on the ability to control their programmed differentiation. So far, it is commonly believed that the differentiation behavior of stem cells is supposed to be identical when they are cultured on the same homogeneous platform. However, in this report, we show that this is not always true. By utilizing a double-ion-triggered shape memory effect, the pre-seeded hMSCs were controllably located in different growth positions. Here, we demonstrate for the first time that the differentiation behavior of hMSCs is highly sensitive to their growth position on a hydrogel scaffold. This work will not only enrich the mechanisms for controlling the differentiation of stem cells, but also offer a one-of-a-kind platform to achieve a heterogeneously differentiated stem cell-seeded hydrogel scaffold for complex biological applications.
authors: 1] Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin 300072, P. R. China [2].
authors: Yanjiao
authors: Y
authors: Han
authors: 1] Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin 300072, P. R. China [2].
authors: Tao
authors: T
authors: Bai
authors: Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin 300072, P. R. China.
authors: Wenguang
authors: W
authors: Liu
mesh_terms: D000179:Acrylates / Q000737:chemistry; D000181:Acrylonitrile / Q000737:chemistry; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002122:Calcium Chloride / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002712:Chlorides / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D054802:Molecular Imprinting / Q000379:methods; D013439:Sulfhydryl Reagents / Q000737:chemistry; D046509:Tissue Culture Techniques; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D000179:Acrylates; D015415:Biomarkers; D002712:Chlorides; D003432:Cross-Linking Reagents; D020100:Hydrogels; D013439:Sulfhydryl Reagents; D017967:Zinc Compounds; C016837:zinc chloride; C036658:acrylic acid; D002122:Calcium Chloride; D000181:Acrylonitrile