title: Screening of endophytic bacteria against fungal plant pathogens.
abstract: Bacterial endophytes were found from 6 plant leaves among 35 plant leaves screened. Two of the isolated bacteria showed antagonistic activity against fungal plant pathogens. An isolate named KL1 showed the clear inihibition against plant pathogens, Fusarium oxysporum and Rhizoctonia solani, on PDA as well as TSA plate. Supernatant of the bacterial culture also showed the clear inhibition against the fungal growth on the plate and the antibiotic substance was identified as iturin A by HPLC analysis. KL1 was identified as Bacillus sp. from the 16S rRNA gene analysis. Very thin hyphae of R. solani was miccroscopically observed when the fungus was co-cultivated with KL1.
authors: Graduate School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan.  bm0031ot@waka.kindai.ac.jp
authors: Tatsuya
authors: T
authors: Ohike
authors: Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan.
authors: Kohei
authors: K
authors: Makuni
authors: Graduate School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan; Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan.
authors: Masahiro
authors: M
authors: Okanami
authors: Graduate School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan; Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan.
authors: Takashi
authors: T
authors: Ano
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D060026:Endophytes / Q000502:physiology*; D005658:Fungi / Q000502:physiology*; D055666:Lipopeptides / Q000032:analysis; D018515:Plant Leaves / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D013696:Temperature
chemical_list: D055666:Lipopeptides
keywords: biocontrol; endophyte; plant pathogens