title: Analysis of immune-related ESTs and differential expression analysis of few important genes in lines of rohu (Labeo rohita) selected for resistance and susceptibility to Aeromonas hydrophila infection.
abstract: A total of 137,629 contigs generated via de novo transcriptome assembly from resistant and susceptible lines of rohu (first generation) raised against aeromoniasis were further analyzed in terms of defence-related genes. Out of 1,939 contigs showing homology to genes involved in immune processes, 1,866 were further categorised into different functional subgroups. Comparative analysis revealed five genes for the first time in any carp species out of which apolipoprotein h, septin 4 isoform 3 and septin isoform cra_c were identified for the first time in fish. Differential expression analysis of ten genes viz., heat shock proteins (Hsps) (Hsp30, Hsp70 and Hsp90), serum lectin isoform 1 (SLI1), linker histone H1M (LHH1M), NAD(P)H quinone 1 (NQO1), zona pellucida 2 (ZP2) and three unknown genes that were highly up-expressed in first generation resistant line fish from mRNA-seq coverage data, was carried out using susceptible and resistant individuals of the second generation selected populations in eight different tissues viz. liver, kidney, intestine, gill, brain, spleen, skin and muscle using qPCR. Significant up-regulation in Hsp90, NQO1, C_116914 and C_22454 in specific tissues of resistant line and variable expression in Hsp30 and LHH1M genes in different tissues of both lines were noticed. The expression of Hsp70 was lower in many tissues of the resistant line than in susceptible line rohu. The expression of ZP2, SLI1 and C_94589 genes was not significantly different in terms of fold difference between the two lines. Differentially expressed genes need further characterisation to explore their role in resistance to Aeromonas hydrophila infection in rohu.
authors: Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751 002, India.
authors: Sweta
authors: S
authors: Das
authors: Chiranjibi
authors: C
authors: Chhottaray
authors: Kanta
authors: K
authors: Das Mahapatra
authors: Jatindra Nath
authors: JN
authors: Saha
authors: Matthew
authors: M
authors: Baranski
authors: Nicholas
authors: N
authors: Robinson
authors: P K
authors: PK
authors: Sahoo
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D017756:Aquaculture; D003530:Cyprinidae; D017931:DNA Primers / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology; D020224:Expressed Sequence Tags / Q000378:metabolism*; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology*; D020869:Gene Expression Profiling / Q000662:veterinary; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D006360:Heat-Shock Proteins / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D017931:DNA Primers; D006360:Heat-Shock Proteins