title: Effect of spinal cord extracts after spinal cord injury on proliferation of rat embryonic neural stem cells and Notch signal pathway in vitro.
abstract: OBJECTIVE
  To investigate the effect of the spinal cord extracts (SCE) after spinal cord injuries (SCIs) on the proliferation of rat embryonic neural stem cells (NSCs) and the expressions of mRNA of Notch1 as well as of Hes1 in this process in vitro.
  
  
  METHODS
  The experiment was conducted in 4 different mediums: NSCs+PBS (Group A-blank control group), NSCs+SCE with healthy SD rats (Group B-normal control group), NSCs+SCE with SD rats receiving sham-operation treatment (Group C-sham-operation group) and NSCs+ SCE with SCIs rats (Group D-paraplegic group). Proliferative abilities of 4 different groups were analyzed by MTT chromatometry after co-culture for 1, 2, 3, 4 and 5 d, respectively. The expressions of Notch1 and Hes1 mRNA were also detected with RT-PCR after co-culture for 24 and 48 h, respectively.
  
  
  RESULTS
  After co-culture for 1, 2, 3, 4 and 5 d respectively, the MTT values of group D were significantly higher than those of group A, group B and group C (P<0.05). However, there were no significantly differences regarding MTT values between group A, group B and group C after co-culture for 1, 2, 3, 4 and 5 d, respectively (P>0.05). Both the expressions of Notch1 and Hes1 mRNA of group D were significantly higher than those of other 3 groups after co-culture for 24 h and 48 h as well (P<0.05). But there was no difference oin expressions of Notch1 and Hes1 mRNA among group A, group B and group C after co-culture for 24 h and 48 h (P>0.05). There was no difference in expressions of Notch1 and Hes1 mRNA between 24 h and 48 h treatment in group D.
  
  
  CONCLUSIONS
  SCE could promote the proliferation of NSCs. It is demonstrated that the microenvironment of SCI may promote the proliferation of NSCs. Besides, SCE could increase the expression of Notch1 and Hes1 mRNA of NSC. It can be concluded that the Notch signaling pathway activation is one of the mechanisms that locally injured microenvironment contributes to the proliferation of ENSC after SCIs. This process may be performed by up-regulating the expressions of Notch1 and Hes1 gene.
authors: Department of Spine Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors: Qing-Zhong
authors: QZ
authors: Zhou
authors: Department of Spine Surgery, Popele's Hospital of Luzhou, Luzhou 646000, China.
authors: Ge
authors: G
authors: Zhang
authors: Department of Spine Surgery, Affiliated TCM Hospital of Luzhou Medical College, Luzhou 646000, China.
authors: Hai-Bo
authors: HB
authors: Long
authors: Department of Spine Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors: Fei
authors: F
authors: Lei
authors: Department of Spine Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors: Fei
authors: F
authors: Ye
authors: Department of Spine Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors: Xu-Feng
authors: XF
authors: Jia
authors: Department of Spine Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors: Yun-Long
authors: YL
authors: Zhou
authors: Department of Spine Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors: Jian-Ping
authors: JP
authors: Kang
authors: Department of Spine Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China. Electronic address: fdxlz2002@163.com.
authors: Da-Xiong
authors: DX
authors: Feng
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002457:Cell Extracts / Q000494:pharmacology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013116:Spinal Cord / Q000737:chemistry*; D013119:Spinal Cord Injuries / Q000378:metabolism*; D000072056:Transcription Factor HES-1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002457:Cell Extracts; D018398:Homeodomain Proteins; D051880:Receptors, Notch; D000072056:Transcription Factor HES-1; C489735:Hes1 protein, rat
keywords: Cell culture; Neural stem cell; Notch signal pathway; Spinal cord extract