title: Immunopathological aspects of experimental Trypanosoma cruzi reinfections.
abstract: Chagas disease is caused by Trypanosoma cruzi infection. Besides the host-related factors, such as immune response and genetic background, the parasite, strain, and occurrences of reinfection episodes, may influence disease outcome. Our results demonstrate that both the primary infection and the reinfection with the Colombiana strain are connected with lower survival rate of the mice. After reinfection, parasitaemia is approximately ten times lower than in primary infected animals. Only Colombiana, Colombiana/Colombiana, and Y/Colombiana groups presented amastigote nests in cardiac tissue. Moreover, the mice infected and/or reinfected with the Colombiana strain had more T. cruzi nests, more intense inflammatory infiltrate, and higher in situ expression of TNF-α and IFN-γ than Y strain. Antigen-stimulated spleen cells from infected and/or reinfected animals produced higher levels of TNF-α, IFN-γ, and IL-10. Our results reinforce the idea that Chagas disease outcome is influenced by the strain of the infective parasite, being differentially modulated during reinfection episodes. It highlights the need of control strategies involving parasite strain characterization in endemic areas for Chagas disease.
authors: Laboratory of Immunology, Federal University of Triângulo Mineiro, Cefores, Frei Paulino Street, 30, 38025-180 Uberaba, MG, Brazil.
authors: Juliana
authors: J
authors: Reis Machado
authors: Laboratory of Immunology, Federal University of Triângulo Mineiro, Cefores, Frei Paulino Street, 30, 38025-180 Uberaba, MG, Brazil.
authors: Marcos Vinícius
authors: MV
authors: Silva
authors: Discipline of Cell Biology, Federal University of Triângulo Mineiro, Frei Paulino Street, 30, 38025-180 Uberaba, MG, Brazil.
authors: Diego Costa
authors: DC
authors: Borges
authors: Laboratory of Immunology, Federal University of Triângulo Mineiro, Cefores, Frei Paulino Street, 30, 38025-180 Uberaba, MG, Brazil.
authors: Crislaine Aparecida
authors: CA
authors: da Silva
authors: Discipline of Parasitology, Federal University of Triângulo Mineiro, Frei Paulino Street, 30, 38025-180 Uberaba, MG, Brazil.
authors: Luis Eduardo
authors: LE
authors: Ramirez
authors: Discipline of Pathology, Federal University of Triângulo Mineiro, Frei Paulino Street, 30, 38025-180 Uberaba, MG, Brazil.
authors: Marlene Antônia
authors: MA
authors: dos Reis
authors: Technical School of Health, Federal University of Paraíba, 58051-900 João Pessoa, PB, Brazil.
authors: Lúcio Roberto
authors: LR
authors: Castellano
authors: Laboratory of Immunology, Federal University of Triângulo Mineiro, Cefores, Frei Paulino Street, 30, 38025-180 Uberaba, MG, Brazil.
authors: Virmondes
authors: V
authors: Rodrigues
authors: Laboratory of Immunology, Federal University of Triângulo Mineiro, Cefores, Frei Paulino Street, 30, 38025-180 Uberaba, MG, Brazil.
authors: Denise Bertulucci Rocha
authors: DB
authors: Rodrigues
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000276:immunology* / Q000469:parasitology / Q000473:pathology*; D016207:Cytokines / Q000096:biosynthesis; D008297:Male; D008810:Mice, Inbred C57BL; D018512:Parasitemia / Q000276:immunology / Q000473:pathology; D016019:Survival Analysis; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D016207:Cytokines