title: Activation of the leukemia plasmacytoid dendritic cell line PMDC05 by Toho-1, a novel IDO inhibitor.
abstract: OBJECTIVE
  Indoleamine-2,3-dioxygenase (IDO) is a rate-limiting enzyme for tryptophan metabolism and plays an immunosuppressive role. Antigen-presenting cells, when activated, increase the expression of IDO, which results in the suppression of subsequent immune reaction. A novel IDO inhibitor, Toho-1, was explored for its applicability to immunotherapy.
  
  
  METHODS
  We investigated the effects of Toho-1 on antigen presentation and antigen-specific cytotoxic T-lymphocyte-inducing ability of leukemia plasmacytoid dendritic cell line PMDC05, which was established in our laboratory.
  
  
  RESULTS
  While antigen presentation-associated molecules in PMDC05 cells were increased by stimulation with lipopolysaccharide and interferon-γ, IDO mRNA and protein expression were also enhanced. Such treatment of PMDC05 cells in combination with Toho-1 enhanced the antigen-presenting and CTL-inducing ability of PMDC05 cells.
  
  
  CONCLUSIONS
  These findings suggest the ability of Toho-1 to potentiate antigen-presenting cells and its applicability in immunotherapy of cancer.
authors: Laboratory of Hematology and Oncology, Graduate School of Health Science, Niigata University, Niigata, Japan.
authors: Akie
authors: A
authors: Yamahira
authors: Laboratory of Hematology and Oncology, Graduate School of Health Science, Niigata University, Niigata, Japan.
authors: Miwako
authors: M
authors: Narita
authors: Laboratory of Hematology and Oncology, Graduate School of Health Science, Niigata University, Niigata, Japan.
authors: Minami
authors: M
authors: Iwabuchi
authors: Laboratory of Hematology and Oncology, Graduate School of Health Science, Niigata University, Niigata, Japan.
authors: Takayoshi
authors: T
authors: Uchiyama
authors: Laboratory of Hematology and Oncology, Graduate School of Health Science, Niigata University, Niigata, Japan.
authors: Shunpei
authors: S
authors: Iwaya
authors: Laboratory of Hematology and Oncology, Graduate School of Health Science, Niigata University, Niigata, Japan.
authors: Rie
authors: R
authors: Ohiwa
authors: Laboratory of Hematology and Oncology, Graduate School of Health Science, Niigata University, Niigata, Japan.
authors: Yoshinori
authors: Y
authors: Nishizawa
authors: Department of Pharmaceutical Sciences, Toho University, Chiba, Japan.
authors: Takafumi
authors: T
authors: Suzuki
authors: Department of Pharmaceutical Sciences, Toho University, Chiba, Japan.
authors: Yusaku
authors: Y
authors: Yokoyama
authors: Division of Hematology, Nagaoka Red Cross Hospital, Nagaoka, Japan.
authors: Shigeo
authors: S
authors: Hashimoto
authors: Division of Hematology, Endocrinology and Metabolism, Department of Internal Medicine, Niigata University, Niigata, Japan.
authors: Jun
authors: J
authors: Takizawa
authors: Division of Hematology, Endocrinology and Metabolism, Department of Internal Medicine, Niigata University, Niigata, Japan.
authors: Hirohito
authors: H
authors: Sone
authors: Laboratory of Hematology and Oncology, Graduate School of Health Science, Niigata University, Niigata, Japan matak@clg.niigata-u.ac.jp.
authors: Masuhiro
authors: M
authors: Takahashi
mesh_terms: D002243:Carbolines / Q000494:pharmacology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000037:antagonists & inhibitors*; D007938:Leukemia / Q000276:immunology*; D013867:Thiohydantoins / Q000494:pharmacology*
chemical_list: D002243:Carbolines; D004791:Enzyme Inhibitors; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D013867:Thiohydantoins; C000592934:Toho-1 compound
keywords: CTL-inducing ability; Necrostatin-1®; Novel IDO inhibitor; PMDC05; Toho-1; antigen-presenting ability; leukemic plasmacytoid dendritic cell line