title: Differential DNA methylation status between breast carcinomatous and normal tissues.
abstract: Breast cancer has been considered to be a multifactorial disease with a wide array of well-characterized gene mutations and chromosomal abnormalities. However, it is becoming evident that the onset or development of breast cancer also depends on epigenetic factors, although the mechanisms have not been fully elucidated. We performed a genome-wide analysis of DNA methylation of breast carcinomatous tissues and paired normal tissues to examine the differences in methylation between them. Methylation-specific polymerase chain reaction (MSP) was used to validate the hypermethylated genes screened out by DNA methylation microarray. We found that hypomethylation and hypermethylation occurred in 2753 and 1795 genes, respectively, in breast carcinomatous tissues. Meanwhile, gene ontology analysis and ingenuity pathway analysis revealed the function and pathway of several genes whose methylation status was altered in breast carcinomatous tissues. In addition, we investigated the promoter methylation status of four genes in breast carcinomatous tissue and paired normal tissues (n=30) by MSP. Promoter hypermethylation of CRABP1, HOXB13, IFNGR2, and PIK3C3 was found in 37% (11/30), 23% (7/30), 17% (5/30), and 2% (2/30) of the carcinomas, respectively. Mutation of these four important genes was critical to many types of cancer. Our results suggest that DNA methylation mechanisms may be involved in regulating the occurrence and development of breast cancer.
authors: Department of Breast Surgery, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China.
authors: Fengliang
authors: F
authors: Wang
authors: Department of Radiology, the Second Affiliated Hospital, Nanjing Medical University, Nanjing, China.
authors: Yafang
authors: Y
authors: Yang
authors: Department of Breast Surgery, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China.
authors: Ziyi
authors: Z
authors: Fu
authors: First Clinical Medicine College, Nanjing University of Chinese Medicine, Nanjing, China.
authors: Nan
authors: N
authors: Xu
authors: Department of Breast Surgery, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China.
authors: Fei
authors: F
authors: Chen
authors: Department of Breast Surgery, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China.
authors: Hong
authors: H
authors: Yin
authors: Jinling High School, Nanjing, China.
authors: Xun
authors: X
authors: Lu
authors: Department of Breast Surgery, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China.
authors: Rong
authors: R
authors: Shen
authors: Department of Breast Surgery, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China. Electronic address: xianqu1981@126.com.
authors: Cheng
authors: C
authors: Lu
mesh_terms: D001940:Breast / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation / Q000502:physiology*; D005260:Female; D053263:Gene Regulatory Networks / Q000502:physiology*; D006801:Humans; D008875:Middle Aged
keywords: Breast cancer; DNA methylation; Microarray