title: Analgesic, neuropharmacological, anti-diarrheal, and cytotoxic activities of the extract of Solanum sisymbriifolium (Lam.) leaves.
abstract: OBJECTIVE
  The present study was undertaken to evaluate the possible analgesic, neuropharmacological, anti-diarrheal, and cytotoxic activities of the ethanol extract of leaves of Solanum sisymbriifolium Lam. (Family: Solanaceae).
  
  
  METHODS
  The analgesic activity was measured by acetic acid-induced writhing inhibition test. The neuropharmacological activities were evaluated using hole cross, hole board, and elevated plus-maze test and the anti-diarrheal activity was assessed using castor oil-induced diarrhea inhibition method. Brine shrimp lethality bioassay was carried out for assessing the cytotoxicity of the ethanol extract of the leaves. Except cytotoxic activity, all the tests were conducted on mice.
  
  
  RESULTS
  The extract at oral doses of 200 and 400 mg/kg body weight showed highly significant (p<0.001) decrease in number of writhing, 52.1±0.66 and 4.4±0.64 compared with the control (78.6±0.29) with the percentage of inhibitions of writhing response were found to be 33.72% and 94.40%, respectively. Compare with the control, the extract at both doses showed significant sedative effect in hole cross test. In hole board test, the extract exhibited highly significant (p<0.001) anxiolytic activity at dose of (200 mg/kg), while the same activity was observed at dose of 400 mg/kg in elevated plus-maze test. The extract showed highly significant (p<0.001) anti-diarrheal activity in a dose-dependent manner. With the extract, significant lethality to brine shrimp was found with LC50 value of 61.66±0.9 μg/ml, which was comparable with the positive control (LC50: 11.89±0.8 µg/ml).
  
  
  CONCLUSIONS
  The results from the present studies support the traditional uses of this plant part and could form the basis of further investigation including compound isolation.
authors: Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh.
authors: Apurba Sarker
authors: AS
authors: Apu
authors: Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh.
authors: Shakhawat Hossan
authors: SH
authors: Bhuyan
authors: Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh.
authors: Maima
authors: M
authors: Matin
authors: Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh.
authors: Faruq
authors: F
authors: Hossain
authors: Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh.
authors: Farjana
authors: F
authors: Khatun
authors: Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh.
authors: Abu
authors: A
authors: Taiab
authors: Department of Pharmacy, East West University, Plot No-A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, Bangladesh.
authors: Jamaluddin
keywords: Analgesic; Anti-diarrheal; Anxiolytic; Cytotoxic; Sedative Activity. Solanum sisymbriifolium