title: Discrepant HIV results resolved by human DNA testing.
abstract: A high-risk patient was informed of a positive HIV antibody/antigen test. However, follow-up samples taken 2-3 months later for HIV RNA and anti-HIV antibodies were negative. Human DNA testing confirmed that all samples were from this patient, excluding a sample mix-up. Laboratory investigations revealed a likely splash-over contamination event.
authors: Alberta Provincial Laboratory for Public Health, Edmonton, Alberta, Canada; Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada. Electronic address: Julian.Tang@albertahealthservices.ca.
authors: Julian W
authors: JW
authors: Tang
authors: Alberta Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
authors: Dena
authors: D
authors: Adachi
authors: Alberta Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
authors: Hans
authors: H
authors: Gunning
authors: Alberta Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
authors: Carol
authors: C
authors: Marian-Dyer
authors: Department of Medical Genetics, University of Alberta, Edmonton, Alberta, Canada.
authors: Stacey
authors: S
authors: Hume
authors: Department of Medical Genetics, University of Alberta, Edmonton, Alberta, Canada.
authors: Sherryl
authors: S
authors: Taylor
authors: Department of Medical Genetics, University of Alberta, Edmonton, Alberta, Canada.
authors: Shelagh
authors: S
authors: Haase
authors: Department of Medical Genetics, University of Alberta, Edmonton, Alberta, Canada.
authors: Leanne
authors: L
authors: Vicen
authors: Alberta Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
authors: Marlin
authors: M
authors: Benedet
authors: Alberta Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
authors: Anthony
authors: A
authors: Chow
authors: Department of Medical Genetics, University of Alberta, Edmonton, Alberta, Canada.
authors: Martin
authors: M
authors: Somerville
authors: Department of Medical Genetics, University of Alberta, Edmonton, Alberta, Canada.
authors: Mark
authors: M
authors: Hicks
authors: Alberta Provincial Laboratory for Public Health, Edmonton, Alberta, Canada; Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada.
authors: Graham
authors: G
authors: Tipples
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods* / Q000592:standards; D003951:Diagnostic Errors; D015483:HIV Antibodies / Q000097:blood; D015488:HIV Antigens / Q000097:blood; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012367:RNA, Viral / Q000097:blood; D055815:Young Adult
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens; D012367:RNA, Viral
keywords: Cross-contamination; Forensic; Microsatellite; Mix-up; Sensitivity; Short tandem repeats