title: Microbial and metabolic characterization of a denitrifying phosphorus-uptake/side stream phosphorus removal system for treating domestic sewage.
abstract: In this study, an advanced wastewater treatment process, the denitrifying phosphorus/side stream phosphorus removal system (DPR-Phostrip), was developed for the purpose of enhancing denitrifying phosphorus removal. The enrichment of denitrifying phosphorus-accumulating organisms (DPAOs) and the microbial community structure of DPR-Phostrip were evaluated by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), and the metabolic activity of seed sludge and activated sludge collected after 55 days of operation were evaluated by Biolog™ analysis. This experimental study of DPR-Phostrip operation showed that nutrients were removed effectively, and denitrifying phosphorus removal was observed during the pre-anoxic period. PCR-DGGE analysis indicated that DPR-Phostrip supported DPAO growth while inhibiting PAOs and GAOs. The major dominant species in DPR-Phostrip were Bacteroidetes bacterium, Saprospiraceae bacterium, and Chloroflexi bacterium. Moreover, the functional diversity indices calculated on the basis of Biolog analysis indicated that DPR-Phostrip had almost no effect on microbial community diversity but was associated with a shift in the dominant species, which confirms the results of the PCR-DGGE analysis. The results for average well color development, calculated via Biolog analysis, showed that DPR-Phostrip had a little impact on the metabolic activity of sludge. Further principal component analysis suggested that the ability to utilize low-molecular-weight organic compounds was reduced in DPR-Phostrip.
authors: College of Urban Construction and Environmental Engineering, Chongqing University (B Campus), No. 174 Shazheng Street, Shapingba District, Chongqing, 400030, China, 568032924@qq.com.
authors: Zhan
authors: Z
authors: Jin
authors: Fang-Ying
authors: FY
authors: Ji
authors: Xuan
authors: X
authors: Xu
authors: Xiao-Yi
authors: XY
authors: Xu
authors: Qing-Kong
authors: QK
authors: Chen
authors: Qi
authors: Q
authors: Li
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D058645:Denaturing Gradient Gel Electrophoresis; D010758:Phosphorus / Q000378:metabolism*; D045483:Rivers / Q000737:chemistry / Q000382:microbiology; D012722:Sewage / Q000382:microbiology*
chemical_list: D012722:Sewage; D010758:Phosphorus