title: Expression of chemotaxis- and angiogenesis-related factors in human monocytes following interaction with colon cancer cells is suppressed by low-dose lipopolysaccharide.
abstract: BACKGROUND
  We have previously reported that mRNA expression of chemotaxis- and angiogenesis-related factors in human monocytes increased following interaction with colon cancer cells. Recently, it was also reported that mRNA expression of the chemotaxis-related factor, monocyte chemotactic protein (MCP)-1, in mouse macrophages following treatment with low-dose lipopolysaccharide (LPS) was significantly lower compared to that following treatment with high-dose LPS, and that low-dose LPS failed to activate the classical nuclear factor (NF)-κB pathway. In the present study, we examined changes in mRNA expression of chemotaxis- and angiogenesis-related factors in human monocytes following low-dose LPS treatment and subsequent interaction with colon cancer cells.
  
  
  METHODS
  The human monocyte cell line THP-1 was treated with LPS and subsequently co-cultured with the human colon cancer cell line DLD-1. mRNA expression was analyzed by quantitative real-time PCR.
  
  
  RESULTS
  mRNA expression of MCP-1, vascular endothelial growth factor (VEGF)-A, tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-8 in THP-1 cells treated with low-dose LPS (100 pg/ml) decreased compared to untreated THP-1 cells after five days of co-culture with DLD-1 cells.
  
  
  CONCLUSIONS
  mRNA expression of chemotaxis- and angiogenesis-related factors in human monocytes following interaction with colon cancer cells is suppressed by prior treatment with low-dose LPS. Thus, low-dose LPS treatment of human monocytes may be useful for prevention and therapy of colon cancer.
authors: Department of Medical Technology, School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa, Japan hondat@azabu-u.ac.jp.
authors: Teruko
authors: T
authors: Honda
authors: Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University, Kida-gun, Kagawa, Japan.
authors: Hiroyuki
authors: H
authors: Inagawa
authors: Department of Medical Technology, School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa, Japan.
authors: Isamu
authors: I
authors: Yamamoto
mesh_terms: D002450:Cell Communication; D018932:Chemokine CCL2 / Q000235:genetics*; D003110:Colonic Neoplasms / Q000473:pathology / Q000628:therapy*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009000:Monocytes / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D016209:Interleukin-8; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A
keywords: Monocyte; co-culture; colon cancer cell; low-dose lipopolysaccharide