title: A method for routine analysis of urinary 4,4'-methylenebis (2-chloroaniline) by gas chromatography-electron capture detection.
abstract: OBJECTIVE
  The purpose of this study was to develop a simple and cost-effective method for the determination of urinary 4,4'-methylenebis (2-chloroaniline) (MBOCA) by gas chromatography-electron capture detection (GC-ECD) for biological monitoring of occupational exposure to MBOCA.
  
  
  METHODS
  MBOCA was prepared by liquid-liquid extraction after alkaline hydrolysis, derivatized with N-methyl-bis (trifluoroacetamide) and then analyzed using GC-ECD. The proposed method was validated in accordance with the US Food and Drug Administration guidance.
  
  
  RESULTS
  The calibration curve showed linearity in the range 1-100 µg/l, with a correlation coefficient of >0.999. The limits of detection and quantification were 0.3 µg/l and 1 µg/l, respectively. The recovery was 94-99%. Intraday accuracy, expressed as the deviation from the nominal value, was 90.5-100.3%, and intraday precision, expressed as the relative standard deviation, was 0.3-2.4%. Interday accuracy and precision were 87.8-100.2% and 0.3-4.1%, respectively.
  
  
  CONCLUSIONS
  The proposed method is a simple and cost-effective method suitable for routine analyses and could be useful for biological monitoring of occupational exposure to MBOCA.
authors: Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association.
authors: Akito
authors: A
authors: Takeuchi
authors: Akira
authors: A
authors: Namera
authors: Norihiro
authors: N
authors: Sakui
authors: Kenji
authors: K
authors: Yamamuro
authors: Toshio
authors: T
authors: Kawai
authors: Kimiaki
authors: K
authors: Sumino
mesh_terms: D001559:Benzhydryl Compounds / Q000652:urine*; D002849:Chromatography, Gas; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D008753:Methylenebis(chloroaniline) / Q000032:analysis* / Q000378:metabolism*; D016273:Occupational Exposure / Q000032:analysis*; D014481:United States; D014556:Urine / Q000737:chemistry*
chemical_list: D001559:Benzhydryl Compounds; D008753:Methylenebis(chloroaniline)