title: Comparative analysis of Hsp10 and Hsp90 expression in healthy mucosa and adenocarcinoma of the large bowel.
abstract: BACKGROUND
  Heat shock proteins (Hsps) assist other proteins in their folding and drive the degradation of defective proteins. During evolution, these proteins have also acquired other roles. Hsp10 is involved in immunomodulation and tumor progression. Hsp90 stabilizes a range of "client" proteins involved in cell signaling. The present study evaluated the expression levels of Hsp10 and Hsp90 in normal mucosa and adenocarcinoma samples of human large bowel.
  
  
  METHODS
  Samples of normal mucosa and adenocarcinoma were collected and Reverse transcriptase-polymerase chain reaction RT-PCR, western blotting (WB) analyses, as well as immunohistochemistry were performed to evaluate the expression levels of Hsp10 and Hsp90.
  
  
  RESULTS
  RT-PCR showed a higher gene expression of Hsp10 and Hsp90 in adenocarcinoma samples compared to healthy mucosa. WB results confirmed these findings. Immunohistochemistry revealed higher levels of Hsp10 in adenocarcinoma in both the epithelium and the lamina propria, while Hsp90 expression was higher in the adenocarcinoma samples only in the lamina propria.
  
  
  CONCLUSIONS
  Hsp10 and Hsp90 may be involved in large bowel carcinogenesis.
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy francyrappa@hotmail.com.
authors: Francesca
authors: F
authors: Rappa
authors: Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy.
authors: Carmelo
authors: C
authors: Sciume
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
authors: Margherita
authors: M
authors: Lo Bello
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
authors: Celeste Caruso
authors: CC
authors: Bavisotto
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors: Antonella
authors: A
authors: Marino Gammazza
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors: Rosario
authors: R
authors: Barone
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors: Claudia
authors: C
authors: Campanella
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
authors: Sabrina
authors: S
authors: David
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
authors: Francesco
authors: F
authors: Carini
authors: Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy.
authors: Federica
authors: F
authors: Zarcone
authors: Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy.
authors: Stefano
authors: S
authors: Rizzuto
authors: Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy.
authors: Adriana
authors: A
authors: Lena
authors: Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy.
authors: Giovanni
authors: G
authors: Tomasello
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Histology and Embryology, University of Palermo, Palermo, Italy.
authors: Maria Laura
authors: ML
authors: Uzzo
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Histology and Embryology, University of Palermo, Palermo, Italy.
authors: Giovanni Francesco
authors: GF
authors: Spatola
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Histology and Embryology, University of Palermo, Palermo, Italy.
authors: Giuseppe
authors: G
authors: Bonaventura
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Histology and Embryology, University of Palermo, Palermo, Italy.
authors: Angelo
authors: A
authors: Leone
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Histology and Embryology, University of Palermo, Palermo, Italy.
authors: Aldo
authors: A
authors: Gerbino
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors: Francesco
authors: F
authors: Cappello
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
authors: Fabio
authors: F
authors: Bucchieri
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
authors: Giovanni
authors: G
authors: Zummo
authors: Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
authors: Felicia
authors: F
authors: Farina
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000209:etiology; D015153:Blotting, Western; D018835:Chaperonin 10 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000209:etiology; D018841:HSP90 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018835:Chaperonin 10; D018841:HSP90 Heat-Shock Proteins
keywords: Hsp10; Hsp90; RT-PCR; immunohistochemistry; large bowel adenocarcinoma