title: Lifespan regulation under axenic dietary restriction: a close look at the usual suspects.
abstract: In Caenorhabditis elegans, there are several ways to impose dietary restriction (DR) all of which extend lifespan to a different degree. Until recently, the molecular mechanisms underlying the DR-mediated lifespan extension were completely unknown but extensive efforts led to the identification of several key players in this process. Culture in sterile axenic medium is a method of DR (ADR), leading to an impressive doubling of lifespan. Earlier, we established that ADR-mediated longevity is independent of Ins/IGF signaling and eat-2. The only gene reported to be indispensable for the ADR lifespan effect is cbp-1 (Zhang et al., 2009) which was confirmed in this study. In an attempt to identify more genes involved in ADR-mediated longevity, we tested several candidate genes known to regulate lifespan extension in other DR regimens. We found that cup-4 is equally important as cbp-1 in ADR-mediated longevity and we identified some genes that may contribute to ADR-induced longevity, but are not required for the full lifespan effect.
authors: Department of Biology, Ghent University, Proeftuinstraat 86 N1, 9000 Gent, Belgium.
authors: N
authors: N
authors: Castelein
authors: Department of Biology, Ghent University, Proeftuinstraat 86 N1, 9000 Gent, Belgium.
authors: H
authors: H
authors: Cai
authors: Department of Biology, Ghent University, Proeftuinstraat 86 N1, 9000 Gent, Belgium.
authors: M
authors: M
authors: Rasulova
authors: Department of Biology, Ghent University, Proeftuinstraat 86 N1, 9000 Gent, Belgium. Electronic address: Bart.Braeckman@UGent.be.
authors: B P
authors: BP
authors: Braeckman
mesh_terms: D000367:Age Factors; D000818:Animals; D061245:Axenic Culture; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D031204:Caloric Restriction; D004734:Energy Metabolism / Q000235:genetics; D005786:Gene Expression Regulation; D005856:Germ-Free Life; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D008136:Longevity / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C504071:CUP-4 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D014157:Transcription Factors; C111709:CBP-1 protein, C elegans; D051548:Histone Acetyltransferases
keywords: Axenic culture; Caenorhabditis elegans; Dietary restriction; Lifespan extension; Molecular mechanism