title: Abnormal Sensitivity to Glucagon and Related Peptides in Primary Adrenal Cushing's Syndrome.
abstract: Illegitimate G-protein coupled receptors are known to control cortisol secretion in adrenal adenomas and bilateral macronodular adrenal hyperplasias (BMAHs) causing Cushing's syndrome. In the present study, we have evaluated the role of glucagon in the regulation of cortisol secretion in 13 patients with BMAH or adrenocortical adenoma causing subclinical or overt Cushing's syndrome. Injection of glucagon provoked an increase in plasma cortisol in 2 patients. After surgery, immunohistochemical studies showed the presence of glucagon receptor-like immunoreactivity in clusters of spongiocytic cells in adrenal tissues from patients who were sensitive in vivo to glucagon. We also observed an in vitro cortisol response to vasoactive intestinal peptide from an adenoma, which was insensitive to glucagon and pituitary adenylate cyclase-activating peptide. Altogether, our data show that ectopic glucagon receptors are expressed in some adrenal cortisol-producing benign lesions. Our results also indicate that circulating glucagon may influence cortisol release under fasting conditions.
authors: INSERM, U982, Laboratory of Differentiation & Neuronal and Neuroendocrine Communication, IRIB, University of Rouen, Mont-Saint-Aignan, France.
authors: E
authors: E
authors: Louiset
authors: INSERM, U982, Laboratory of Differentiation & Neuronal and Neuroendocrine Communication, IRIB, University of Rouen, Mont-Saint-Aignan, France.
authors: C
authors: C
authors: Duparc
authors: Department of Endocrinology, University Hospital Cochin and Cochin Institute, Assistance Publique des Hôpitaux de Paris, Université Paris V, Paris, France.
authors: L
authors: L
authors: Groussin
authors: Department of Pathology, University Hospital of Rouen, Rouen, France.
authors: F
authors: F
authors: Gobet
authors: Department of Endocrinology, University Hospital of Amiens, Amiens, France.
authors: R
authors: R
authors: Desailloud
authors: Department of Endocrinology, Regional Hospital of Orléans, Orléans, France.
authors: G
authors: G
authors: Barrande
authors: Department of Endocrinology, University Hospital of Caen, Caen, France.
authors: Y
authors: Y
authors: Reznik
authors: Department of Endocrinology, University Hospital Cochin and Cochin Institute, Assistance Publique des Hôpitaux de Paris, Université Paris V, Paris, France.
authors: J
authors: J
authors: Bertherat
authors: INSERM, U982, Laboratory of Differentiation & Neuronal and Neuroendocrine Communication, IRIB, University of Rouen, Mont-Saint-Aignan, France.
authors: G
authors: G
authors: Prévost
authors: INSERM, U982, Laboratory of Differentiation & Neuronal and Neuroendocrine Communication, IRIB, University of Rouen, Mont-Saint-Aignan, France.
authors: H
authors: H
authors: Lefebvre
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000473:pathology*; D018246:Adrenocortical Adenoma / Q000097:blood; D000328:Adult; D000368:Aged; D003480:Cushing Syndrome / Q000097:blood / Q000473:pathology*; D005260:Female; D005934:Glucagon / Q000494:pharmacology*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood / Q000378:metabolism; D006965:Hyperplasia; D007150:Immunohistochemistry; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000494:pharmacology*; D018027:Receptors, Glucagon / Q000378:metabolism; D055815:Young Adult
chemical_list: D010455:Peptides; D018027:Receptors, Glucagon; D005934:Glucagon; D006854:Hydrocortisone