title: Bioflocculant production and biosorption of zinc and lead by a novel bacterial species, Achromobacter sp. TERI-IASST N, isolated from oil refinery waste.
abstract: A bioflocculant-producing bacterial isolate designated as 'TERI-IASST N' was isolated from activated sludge samples collected from an oil refinery. This isolate demonstrated maximum bioflocculation activity (74%) from glucose among 15 different bioflocculant-producing bacterial strains isolated from the sludge samples and identified as Achromobacter sp. based on 16S rRNA gene sequence. Optimization of pH and supplementation of urea as nitrogen source in the production medium enhanced the flocculation activity of strain TERI-IASST N to 84% (at pH 6). This strain revealed maximum flocculation activity (90%) from sucrose compared to the flocculation activity observed from other carbon sources as investigated (glucose, lactose, fructose, maltose and starch). Ca(2+) served as the suitable divalent cation for maximum bioflocculation activity of TERI-IASST strain N. Maximum flocculation activity was observed at optimum C/N ratio of 1. Flocculation activity of this strain decreased to 75% in the presence of heavy metals; Zn, Pb, Ni, Cu and Cd. In addition strain N revealed considerable biosorption of Zn (430mgL(-1)) and Pb (30mgL(-1)). Bioflocculant yield of strain N was 10.5gL(-1). Fourier transform infrared spectrum indicated the presence of carboxyl, hydroxyl, and amino groups, typical of glycoprotein. Spectroscopic analysis of bioflocculant by nuclear magnetic resonance revealed that it is a glycoprotein, consisting of 57% total sugar and 13% protein.
authors: Environmental and Industrial Biotechnology Division, The Energy and Resources Institute, Lodi Road, New Delhi, India. Electronic address: ssubudhi@teri.res.in.
authors: Sanjukta
authors: S
authors: Subudhi
authors: Environmental and Industrial Biotechnology Division, The Energy and Resources Institute, Lodi Road, New Delhi, India.
authors: Neha
authors: N
authors: Batta
authors: Resource Management and Environment Section, Life Science Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, India.
authors: Mihirjyoti
authors: M
authors: Pathak
authors: Environmental and Industrial Biotechnology Division, The Energy and Resources Institute, Lodi Road, New Delhi, India.
authors: Varsha
authors: V
authors: Bisht
authors: Resource Management and Environment Section, Life Science Division, Institute of Advanced Study in Science and Technology, Guwahati, Assam, India.
authors: Arundhuti
authors: A
authors: Devi
authors: Environmental and Industrial Biotechnology Division, The Energy and Resources Institute, Lodi Road, New Delhi, India. Electronic address: banwaril@teri.res.in.
authors: Banwari
authors: B
authors: Lal
authors: Kuwait International Law School, Kuwait International Legal Research Center, Doha City, Kuwait.
authors: Bader
authors: B
authors: Al khulifah
mesh_terms: D042402:Achromobacter / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D005427:Flocculation; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles; D007220:Industrial Waste; D007854:Lead / Q000302:isolation & purification / Q000493:pharmacokinetics*; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D009025:Morpholines; D009584:Nitrogen / Q000378:metabolism; D010578:Petroleum; D013997:Time Factors; D015032:Zinc / Q000302:isolation & purification / Q000493:pharmacokinetics*
chemical_list: C472556:FT 005; D007093:Imidazoles; D007220:Industrial Waste; D009025:Morpholines; D010578:Petroleum; D007854:Lead; D002244:Carbon; D015032:Zinc; D009584:Nitrogen
keywords: Achromobacter sp. strain TERI-IASST N1; Bioflocculant yield; Bioflocculation activity; FT-IR; Heavy metal biosorption; NMR