title: Preparation and electrochromatographic characterization of new chiral β-cyclodextrin poly(acrylamidopropyl) porous layer open tubular capillary columns.
abstract: A novel chiral stationary phase consisting of an amidopropyltrimethylammonium chloride divinylbenzene (APAT-DVB) polymer containing the chiral selector heptakis(2,3-di-O-acetyl-6-O-sulfo)-β-cyclodextrin (HSβCD) has been employed in porous layer open tubular (PLOT) capillary column format with various conditions evaluated to optimize the polymerization and chiral selector immobilization. Scanning electron microscopy demonstrated a near homogenous longitudinal open path in the column with a polymer film of uniform thickness. IR spectroscopy characterized the functional groups of the polymer and energy-dispersive X-ray spectroscopy provided further evidence of the successful polymer modification with HSβCD. Optimum electrochromatographic separation conditions were elaborated with respect to organic solvent content and pH of the background electrolyte. Colum-to-column and long-term reproducibility was excellent. The effectiveness of the new capillary column was demonstrated with the successful separation of d-and l-aspartic acid, d- and l-tyrosine and d-and l-lysine.
authors: School of Chemistry, Monash University, Melbourne, VIC 3800, Australia.
authors: Ahmed
authors: A
authors: Al-Hussin
authors: School of Chemistry, Monash University, Melbourne, VIC 3800, Australia.
authors: Reinhard I
authors: RI
authors: Boysen
authors: School of Chemistry, Monash University, Melbourne, VIC 3800, Australia.
authors: Kei
authors: K
authors: Saito
authors: School of Chemistry, Monash University, Melbourne, VIC 3800, Australia. Electronic address: milton.hearn@monash.edu.
authors: Milton T W
authors: MT
authors: Hearn
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D001224:Aspartic Acid / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000379:methods; D003505:Cyclodextrins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000302:isolation & purification; D008855:Microscopy, Electron, Scanning; D058105:Polymerization; D016062:Porosity; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013499:Surface Properties; D014443:Tyrosine / Q000302:isolation & purification
chemical_list: D000180:Acrylic Resins; D003505:Cyclodextrins; C486937:heptakis(2,3-di-O-acetyl-6-O-sulfo)-beta-cyclodextrin; D001224:Aspartic Acid; D014443:Tyrosine; D008239:Lysine
keywords: Chiral selector; Chiral stationary phase; Electrochromatography; In situ polymerization; Porous layer open tubular