title: Role of SCOX in determination of Drosophila melanogaster lifespan.
abstract: In man, COX (cytochrome c oxidase) deficiency is reported to be related to mutation of the SCO2 (synthesis of cytochrome c oxidase 2) gene, which encodes one of the copper-donor chaperones involved in the assembly of mitochondrial cytochrome c oxidase. Such COX deficiency due to the genetic condition leads to heart disease and the Leigh syndrome and is frequently fatal in childhood. Synthesis of cytochrome c oxidase X (SCOX) is a Drosophila orthologue of human SCO2. Here, we generated SCOX-knockdown flies and the full length SCOX transgenic flies to investigate the in vivo roles of SCOX. Our results demonstrated knockdown of SCOX gene in all cells and tissues to be associated with lethality at larval or pupal stages and this correlated with a decrease in ATP level. In contrast, the full length SCOX transgenic flies showed a longer lifespan than wild type flies and control flies carrying Act5C-GAL4 alone and this correlated with an increase in ATP level. Finally, when cultured on paraquat-added medium, full length SCOX transgenic flies also exhibited an elongated lifespan. Therefore, we hypothesized that SCOX plays an important role in ATP production and consumption, which helps to prevent production of mitochondrial reactive oxygen species and/or impairment of mitochondrial activity under oxidative stress.
authors: Kankyo Eisei Yakuhin Co. Ltd, Kansai Gakken Laboratory Hikaridai, Seika-cho, Souraku-gun, Kyoto 619-0237, Japan ; Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki Sakyo-ku, Kyoto 606-8585, Japan.
authors: Thanh Binh
authors: TB
authors: Nguyen
authors: Kankyo Eisei Yakuhin Co. Ltd, Kansai Gakken Laboratory Hikaridai, Seika-cho, Souraku-gun, Kyoto 619-0237, Japan.
authors: Hiroyuki
authors: H
authors: Ida
authors: Kankyo Eisei Yakuhin Co. Ltd, Kansai Gakken Laboratory Hikaridai, Seika-cho, Souraku-gun, Kyoto 619-0237, Japan.
authors: Mai
authors: M
authors: Shimamura
authors: Kankyo Eisei Yakuhin Co. Ltd, Kansai Gakken Laboratory Hikaridai, Seika-cho, Souraku-gun, Kyoto 619-0237, Japan.
authors: Daishi
authors: D
authors: Kitazawa
authors: Kankyo Eisei Yakuhin Co. Ltd, Kansai Gakken Laboratory Hikaridai, Seika-cho, Souraku-gun, Kyoto 619-0237, Japan.
authors: Shinichi
authors: S
authors: Akao
authors: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki Sakyo-ku, Kyoto 606-8585, Japan ; Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki Sakyo-ku, Kyoto 606-8585, Japan.
authors: Hideki
authors: H
authors: Yoshida
authors: Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki Sakyo-ku, Kyoto 606-8585, Japan.
authors: Yoshihiro H
authors: YH
authors: Inoue
authors: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki Sakyo-ku, Kyoto 606-8585, Japan ; Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki Sakyo-ku, Kyoto 606-8585, Japan.
authors: Masamitsu
authors: M
authors: Yamaguchi
keywords: ATP; COX; ROS; SCO2; SCOX; lifespan