title: Three-dimensional arbitrary trajectory scanning photoacoustic microscopy.
abstract: We have enhanced photoacoustic microscopy with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-to-noise ratio (SNR) despite the depth variation of the vessels. We showed that hemoglobin oxygen saturation (sO2 ) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. We also observed sO2 dynamics in response to switching from systemic hypoxia to hyperoxia. 3-DAT-scanning photoacoustic microscopy. Schematic diagram of the 3D scanning stage and method.
authors: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, One Brookings Dr., St. Louis, MO 63130, USA.
authors: Chenghung
authors: C
authors: Yeh
authors: Brian
authors: B
authors: Soetikno
authors: Song
authors: S
authors: Hu
authors: Konstantin I
authors: KI
authors: Maslov
authors: Lihong V
authors: LV
authors: Wang
mesh_terms: D000818:Animals; D004423:Ear / Q000098:blood supply; D006197:Hair; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D010100:Oxygen / Q000378:metabolism; D061088:Photoacoustic Techniques / Q000295:instrumentation / Q000379:methods*
chemical_list: D006454:Hemoglobins; D010100:Oxygen
keywords: 3-D arbitrary trajectory scanning; Optical-resolution photoacoustic microscopy; hemoglobin oxygen saturation; metabolic rate of oxygen; raster scanning