title: Novel flower-shaped albumin particles as controlled-release carriers for drugs to penetrate the round-window membrane.
abstract: Controlled-release carriers for local drug delivery have attracted increasing attention for inner-ear treatment recently. In this paper, flower-shaped bovine serum albumin (FBSA) particles were prepared by a modified desolvation method followed by glutaraldehyde or heat denaturation. The size of the FBSA particles varied from 10 μm to 100 μm, and most were 50-80 μm. Heat-denatured FBSA particles have good cytocompatibility with a prolonged survival time for L929 cells. The FBSA particles were utilized as carriers to investigate the release behaviors of the model drug - rhodamine B. Rhodamine B showed a sustained-release effect and penetrated the round-window membrane of guinea pigs. We also confirmed the attachment of FBSA particles onto the round-window membrane by microscopy. The FBSA particles, with good biocompatibility, drug-loading capacity, adhesive capability, and biodegradability, may have potential applications in the field of local drug delivery for inner-ear disease treatment.
authors: Second Artillery General Hospital, Beijing, People's Republic of China.
authors: Zhan
authors: Z
authors: Yu
authors: Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, College of Basic Medicine, China Medical University, Shenyang, People's Republic of China.
authors: Min
authors: M
authors: Yu
authors: Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin, People's Republic of China.
authors: Zhimin
authors: Z
authors: Zhou
authors: Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin, People's Republic of China.
authors: Zhibao
authors: Z
authors: Zhang
authors: Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin, People's Republic of China.
authors: Bo
authors: B
authors: Du
authors: Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin, People's Republic of China.
authors: Qingqing
authors: Q
authors: Xiong
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D006168:Guinea Pigs; D008422:Materials Testing; D051379:Mice; D010316:Particle Size; D012235:Rhodamines / Q000737:chemistry / Q000493:pharmacokinetics; D012405:Round Window, Ear / Q000737:chemistry / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity / Q000648:ultrastructure*; D014018:Tissue Distribution
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D012235:Rhodamines; D012710:Serum Albumin, Bovine; C029773:rhodamine B
keywords: bovine serum albumin (BSA); controlled release; local delivery; round-window membrane