title: Metabolic engineering for higher alcohol production.
abstract: Engineering microbial hosts for the production of higher alcohols looks to combine the benefits of renewable biological production with the useful chemical properties of larger alcohols. In this review we outline the array of metabolic engineering strategies employed for the efficient diversion of carbon flux from native biosynthetic pathways to the overproduction of a target alcohol. Strategies for pathway design from amino acid biosynthesis through 2-keto acids, from isoprenoid biosynthesis through pyrophosphate intermediates, from fatty acid biosynthesis and degradation by tailoring chain length specificity, and the use and expansion of natural solvent production pathways will be covered.
authors: Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors: Nicole E
authors: NE
authors: Nozzi
authors: Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA; Microbiology Graduate Group, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors: Shuchi H
authors: SH
authors: Desai
authors: Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors: Anna E
authors: AE
authors: Case
authors: Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA; Microbiology Graduate Group, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. Electronic address: satsumi@ucdavis.edu.
authors: Shota
authors: S
authors: Atsumi
mesh_terms: D000438:Alcohols / Q000378:metabolism*; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005658:Fungi / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods*; D060847:Metabolic Engineering / Q000379:methods*; D064688:Metabolic Flux Analysis / Q000379:methods; D015398:Signal Transduction / Q000502:physiology
chemical_list: D000438:Alcohols; D001426:Bacterial Proteins; D005656:Fungal Proteins
keywords: Biofuel; Butanol; Higher alcohol; Isobutanol