title: Differentiation of industrial sake yeast strains by a loop-mediated isothermal amplification method that targets the PHO3 gene.
abstract: We developed a loop-mediated isothermal amplification method that targets the PHO3 gene for discriminating sake yeast strains. Our data indicate that this assay is simple, rapid, and useful to use for differentiation of specific yeasts in sake mash.
authors: Niigata Prefectural Sake Research Institute, 2-5932-133 Suido-cho, Chuoh-ku, Niigata 951-8121, Japan.
authors: Takashi
authors: T
authors: Kuribayashi
authors: Niigata Prefectural Sake Research Institute, 2-5932-133 Suido-cho, Chuoh-ku, Niigata 951-8121, Japan. Electronic address: sato.keigo@pref.niigata.lg.jp.
authors: Keigo
authors: K
authors: Sato
authors: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
authors: Daisuke
authors: D
authors: Kasai
authors: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
authors: Masao
authors: M
authors: Fukuda
authors: Niigata Prefectural Sake Research Institute, 2-5932-133 Suido-cho, Chuoh-ku, Niigata 951-8121, Japan.
authors: Mitsuoki
authors: M
authors: Kaneoke
authors: Niigata Prefectural Sake Research Institute, 2-5932-133 Suido-cho, Chuoh-ku, Niigata 951-8121, Japan.
authors: Ken-ichi
authors: K
authors: Watanabe
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics*; D000434:Alcoholic Beverages / Q000382:microbiology*; D021141:Nucleic Acid Amplification Techniques; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000135:Acid Phosphatase; C488913:PHO3 protein, S cerevisiae
keywords: Acid phosphatase; Differentiation; Loop-mediated isothermal amplification; PHO3; Sake yeast