title: TCAB1: a potential target for diagnosis and therapy of head and neck carcinomas.
abstract: BACKGROUND
  WRAP53, including α, β and γ isoforms, plays an important role not only in the stability of p53 mRNA, but also in the assembly and trafficking of the telomerase holoenzyme. It has been considered an oncogene and is thought to promote the survival of cancer cells. The aim of this study was to detect the role of TCAB1 (except WRAP53α) in the occurrence and development of head and neck carcinomas.
  
  
  METHODS
  Immunohistochemistry was used to detect the TCAB1 expression in clinical specimen sections and performed western blotting to check the TCAB1 expression levels in cell lines. TCAB1 was depleted using shRNA lentivirus and the knockdown efficiency was assessed using q-PCR and Western blotting. We performed CCK-8 assays and flow cytometry to check the cell proliferation potential and used the trans-well assay to test the invasion ability in vitro. Xenografts were used to detect the tumor formation potential in vivo. Moreover, we performed cDNA microarray to investigate the candidate factors involved in this process.
  
  
  RESULTS
  We observed a notable overexpression of TCAB1 in head and neck carcinoma clinical specimens as well as in carcinoma cell lines. Knockdown of TCAB1 decreased the cellular proliferation potential and invasion ability in vitro. cDNA microarray analysis suggested the possible involvement of several pathways and factors associated with tumorigenesis and carcinoma development in the TCAB1-mediated regulation of cancers. Furthermore, the xenograft assay confirmed that the depletion of TCAB1 would inhibit tumor formation in nude mice. The immunohistochemistry results of the mice tumor tissue sections revealed that the cells in shTCAB1 xenografts showed decreased proliferation potential and increased apoptotic trend, meanwhile, the angiogenesis was inhibited in the smaller tumors form shTCAB1 cells.
  
  
  CONCLUSIONS
  Our study demonstrated that depletion of TCAB1 decreased cellular proliferation and invasion potential both in vitro and in vivo. The data indicated that TCAB1 might facilitate the occurrence and development of head and neck carcinomas. In future, TCAB1 might be useful as a prognostic biomarker or a potential target for the diagnosis and therapy of head and neck carcinomas.
authors: Chong-kui
authors: CK
authors: Sun
authors: Xiao-bo
authors: XB
authors: Luo
authors: Ya-ping
authors: YP
authors: Gou
authors: Ling
authors: L
authors: Hu
authors: Kun
authors: K
authors: Wang
authors: Chao
authors: C
authors: Li
authors: Zhen-ting
authors: ZT
authors: Xiang
authors: Ping
authors: P
authors: Zhang
authors: Xiang-li
authors: XL
authors: Kong
authors: Chao-liang
authors: CL
authors: Zhang
authors: Qin
authors: Q
authors: Yang
authors: Jing
authors: J
authors: Li
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China. xiaoly@scu.edu.cn.
authors: Li-ying
authors: LY
authors: Xiao
authors: Yan
authors: Y
authors: Li
authors: Qian-ming
authors: QM
authors: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000175:diagnosis* / Q000628:therapy*; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D055785:Gene Knockdown Techniques; D006258:Head and Neck Neoplasms / Q000098:blood supply / Q000175:diagnosis* / Q000628:therapy*; D006801:Humans; D008807:Mice, Inbred BALB C; D018832:Molecular Chaperones; D058990:Molecular Targeted Therapy; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction; D000077195:Squamous Cell Carcinoma of Head and Neck; D019098:Telomerase / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D018832:Molecular Chaperones; D034741:RNA, Small Interfering; D019098:Telomerase; C535133:WRAP53 protein, human