title: Comparative analysis of main aromatic acids and phthalides in Angelicae Sinensis Radix, Chuanxiong Rhizoma, and Fo-Shou-San by a validated UHPLC-TQ-MS/MS.
abstract: Fo-Shou-San (FSS) is an ancient and classic formula comprised of Angelicae Sinensis Radix (Danggui, DG) and Chuanxiong Rhizoma (Chuanxiong, CX) in a weight ratio of 3:2 with nourishing blood and dissipating blood stasis activities for the treatment of blood deficiency and blood stasis. In this study, a ultra-high-performance liquid chromatography coupled with a triple quadrupole electrospray tandem mass spectrometry (UHPLC-TQ-MS/MS) method was developed for simultaneous quantification of three aromatic acids (chlorogenic acid, caffeic acid, ferulic acid) and six phthalides (senkyunolide I, senkyunolide H, senkyunolide A, butylphthalide, ligustilide and butylidenephthalide) in DG, CX and FSS. The nine components were simultaneously determined within 10min. The proposed method was fully validated in terms of linearity, sensitivity, precision, repeatability as well as recovery. The results showed that there were significant differences in their contents of DG and CX, and there were remarkable differences between the theorized content and observed content in FSS. The content of each component in formulae was not just the simple addition among its content in the single herbs. These research results might be helpful to illustrate the drug interactions during decocting process of herb pair according to the quantity changes of these marker compounds, which would lay foundation to further reveal the compatibility rule of the herb pair and other related formulae.
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China; The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China.
authors: Weixia
authors: W
authors: Li
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: yupingtang@njutcm.edu.cn.
authors: Yuping
authors: Y
authors: Tang
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors: Yefei
authors: Y
authors: Qian
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors: Erxin
authors: E
authors: Shang
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors: Linyan
authors: L
authors: Wang
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors: Li
authors: L
authors: Zhang
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors: Shulan
authors: S
authors: Su
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: dja@njutcm.edu.cn.
authors: Jin-ao
authors: JA
authors: Duan
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry; D001572:Benzofurans / Q000302:isolation & purification*; D002109:Caffeic Acids / Q000302:isolation & purification*; D002726:Chlorogenic Acid / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D030004:Ligusticum / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D001572:Benzofurans; D002109:Caffeic Acids; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; C000596629:Fo-Shou-San; D002726:Chlorogenic Acid; C082022:phthalide; C004999:ferulic acid; C044613:angelicae sinensis extract; C101574:chuangxiong extract
keywords: Aromatic acid; Fo-Shou-San; Phthalide; UHPLC–TQ-MS/MS