title: Salicornia europaea L. Na⁺/H⁺ antiporter gene improves salt tolerance in transgenic alfalfa (Medicago sativa L.).
abstract: In order to obtain a salt-tolerant perennial alfalfa (Medicago sativa L.), we transferred the halophyte Salicornia europaea L. Na(+)/H(+) antiporter gene, SeNHX1, to alfalfa by using the Agrobacterium-mediated transformation method. The transformants were confirmed by both PCR and RT-PCR analyses. Of 197 plants that were obtained after transformation, 36 were positive by PCR analysis using 2 primer pairs for the CaMV35S-SeNHX1 and SeNHX1-Nos fragments; 6 plants survived in a greenhouse. RT-PCR analysis revealed that SeNHX1 was expressed in 5 plants. The resultant transgenic alfalfa had better salt tolerance. After stress treatment for 21 days with 0.6% NaCl, the chlorophyll and MDA contents in transgenic plants were lower, but proline content and SOD, POD, and CAT activities were higher than those in wild-type plants. These results suggest that the salt tolerance of transgenic alfalfa was improved by the overexpression of the SeNHX1 gene.
authors: Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology, College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors: L Q
authors: LQ
authors: Zhang
authors: Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology, College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors: Y D
authors: YD
authors: Niu
authors: Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology, College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors: H
authors: H
authors: Huridu
authors: Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology, College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors: J F
authors: JF
authors: Hao
authors: Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology, College of Life Sciences, Inner Mongolia University, Hohhot, China.
authors: Z
authors: Z
authors: Qi
authors: Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology, College of Life Sciences, Inner Mongolia University, Hohhot, China hasind@sina.com.
authors: A
authors: A
authors: Hasi
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D027703:Amaranthaceae / Q000737:chemistry / Q000235:genetics*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002734:Chlorophyll / Q000096:biosynthesis; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D008315:Malondialdehyde / Q000378:metabolism; D000455:Medicago sativa / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D055049:Salt Tolerance; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D019076:Transgenes
chemical_list: D010940:Plant Proteins; D017923:Sodium-Hydrogen Exchangers; D002734:Chlorophyll; D012965:Sodium Chloride; D008315:Malondialdehyde; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase