title: A biodistribution study of PEGylated PCL-based nanoparticles in C57BL/6 mice bearing B16/F10 melanoma.
abstract: One of the major drawbacks that limits the clinical application of nanoparticles is the lack of preliminary investigations related to their biocompatibility, biodegradability and biodistribution. In this work, biodegradable PEGylated polymer nanoparticles (NPs) have been synthesized by using macromonomers based on poly(ε-caprolaconte) oligomers. More in detail, NPs have been produced by adopting a surfactant-free semibatch emulsion polymerization process using PEG chains as a stabilizing agent. The NPs were also labeled with rhodamine B covalently bound to the NPs to quantitatively study their biodistribution in vivo. NPs were investigated in both in vitro and in vivo preclinical systems to study their biodistribution in mice bearing B16/F10 melanoma, as well as their biocompatibility and biodegradability. The NP concentration was evaluated in different tissues at several times after intravenous injection. The disappearance of the NPs from the plasma was biphasic, with distribution and elimination half-lives of 30 min and 15 h, respectively. NPs were retained in tumors and in filter organs for a long time, were still detectable after 7 d and maintained a steady concentration in the tumor for 120 h. 48 h after injection, 70 ± 15% of the inoculated NPs were excreted in the feces. The favorable tumor uptake, fast excretion and absence of cytotoxicity foster the further development of produced NPs as drug delivery carriers.
authors: IRCCS-Istituto di Ricerche Farmacologiche Mario Negri, Via La Masa 19, I-20156, Milano, Italy.
authors: M
authors: M
authors: Lupi
authors: C
authors: C
authors: Colombo
authors: R
authors: R
authors: Frapolli
authors: R
authors: R
authors: Ferrari
authors: L
authors: L
authors: Sitia
authors: L
authors: L
authors: Dragoni
authors: E
authors: E
authors: Bello
authors: S A
authors: SA
authors: Licandro
authors: F
authors: F
authors: Falcetta
authors: P
authors: P
authors: Ubezio
authors: P
authors: P
authors: Bigini
authors: M
authors: M
authors: Salmona
authors: M
authors: M
authors: D'Incalci
authors: M
authors: M
authors: Morbidelli
authors: D
authors: D
authors: Moscatelli
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D005260:Female; D008546:Melanoma, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000493:pharmacokinetics*; D011108:Polymers; D012235:Rhodamines / Q000737:chemistry / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D011108:Polymers; D012235:Rhodamines; D011092:Polyethylene Glycols; C029773:rhodamine B