title: mtDNA Diversity and Phylogenetic State of Korean Cattle Breed, Chikso.
abstract: In order to analyze the genetic diversity and phylogenetic status of the Korean Chikso breed, we determined sequences of mtDNA cytochrome b (cyt b) gene and performed phylogenetic analysis using 239 individuals from 5 Chikso populations. Five non-synonymous mutations of a total of 15 polymorphic sites were identified among 239 cyt b coding sequences. Thirteen haplotypes were defined, and haplotype diversity was 0.4709 ranging from 0.2577 to 0.6114. Thirty-five haplotypes (C1-C35) were classified among 9 Asia and 3 European breeds. C2 was a major haplotype that contained 206 sequences (64.6%) from all breeds used. C3-C13 haplotypes were Chikso-specific haplotypes. C1 and C2 haplotypes contained 80.5% of cyt b sequences of Hanwoo, Yanbian, Zaosheng and JB breeds. In phylogenetic analyses, the Chikso breed was contained into B. taurus lineage and was genetically more closely related to two Chinese breeds than to Korean brown cattle, Hanwoo. These results suggest that Chikso and Hanwoo have a genetic difference based on the mtDNA cyt b gene as well as their coat color, sufficient for classification as a separate breed.
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Jae-Hwan
authors: JH
authors: Kim
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Mi Jeong
authors: MJ
authors: Byun
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Myung-Jick
authors: MJ
authors: Kim
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Sang Won
authors: SW
authors: Suh
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Yeoung-Gyu
authors: YG
authors: Ko
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Chang Woo
authors: CW
authors: Lee
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Kyoung-Sub
authors: KS
authors: Jung
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Eun Sung
authors: ES
authors: Kim
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Dae Jung
authors: DJ
authors: Yu
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Woo Hyun
authors: WH
authors: Kim
authors: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors: Seong-Bok
authors: SB
authors: Choi
keywords: Chikso; Cytochrome b; Haplotype; Phylogenetic Analysis; mtDNA