title: Evanescent-field-induced Raman scattering for bio-friendly fingerprinting at sub-cellular dimension.
abstract: Evanescent field induced chemical imaging concept has been realized in analytical platform based on the µ-tip-enhanced Raman scattering spectroscopy (µ-TERS). The technique aimed to minimize thermal decomposition of dried biological sample as the result of huge concentration of optical field near the tip by increasing the size of an aperture-less "excitation source". µ-TERS technique is similar to classical biosensor systems based on propagating surface plasmon resonance phenomenon but with sensitive elements a few micrometers in size that can be targeted to the area of interest. The utility of the concept is exemplified by the analysis of dried single cell envelope of genetically modified Saccharomyces cerevisiae yeast cells, which do not have any heat-removing pathways, by water as in the case of the living cell. Practical excitation conditions effective for µ-TERS Raman observation of single layer dried biological samples without photodamage-related spectral distortion have been determined - the allowable limit is above 30s at 13 µW/µm(2). Finally, potential of µ-TERS spectroscopy as new bio-friendly instrumental platform for chemical fingerprinting and analytical characterization of buried nanoscale features is discussed.
authors: Kaunas University of Technology, Research Centre for Microsystems and Nanotechnology, Studentu 65, 51369 Kaunas, Lithuania; Vilnius University, Institute of Biochemistry, Mokslininkų 12, 08662 Vilnius, Lithuania; V. Ye. Lashkaryov Institute of Semiconductor Physics, National Academy of Sciences of Ukraine, Prospekt Nauky, 41, Kyiv 03028, Ukraine.
authors: Boris
authors: B
authors: Snopok
authors: Kaunas University of Technology, Research Centre for Microsystems and Nanotechnology, Studentu 65, 51369 Kaunas, Lithuania.
authors: Denys
authors: D
authors: Naumenko
authors: Vilnius Gediminas Technical University, Department of Chemistry and Bioengineering, Sauletekio al. 11, LT-10223 Vilnius, Lithuania; Nature Research Centre, Akademijos 2, 08412 Vilnius, Lithuania.
authors: Elena
authors: E
authors: Serviene
authors: Vilnius Gediminas Technical University, Department of Chemistry and Bioengineering, Sauletekio al. 11, LT-10223 Vilnius, Lithuania.
authors: Ingrida
authors: I
authors: Bruzaite
authors: Kaunas University of Technology, Research Centre for Microsystems and Nanotechnology, Studentu 65, 51369 Kaunas, Lithuania.
authors: Andrius
authors: A
authors: Stogrin
authors: Vilnius University, Institute of Biochemistry, Mokslininkų 12, 08662 Vilnius, Lithuania; Vilnius Gediminas Technical University, Department of Chemistry and Bioengineering, Sauletekio al. 11, LT-10223 Vilnius, Lithuania.
authors: Juozas
authors: J
authors: Kulys
authors: Kaunas University of Technology, Research Centre for Microsystems and Nanotechnology, Studentu 65, 51369 Kaunas, Lithuania.
authors: Valentinas
authors: V
authors: Snitka
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000166:cytology; D059010:Single-Cell Analysis / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*; D020349:Surface Plasmon Resonance
keywords: Atomic force microscopy; Cellular envelope; Dried biological samples; Evanescent wave; Raman scattering; Surface plasmon resonance; Tip enhanced Raman spectroscopy; Yeast cell; μ–TERS