title: First report on trichomonads from true bugs.
abstract: Although the hindgut of some insects represents a rich source of intestinal trichomonads, their diversity is only poorly understood. The aim of the present study was to investigate the presence and abundance of intestinal trichomonads in true bugs (Heteroptera). We microscopically examined intestinal contents of more than 780 specimens belonging to 28 families of true bugs from localities in China, Ghana and Papua New Guinea for the presence of intestinal endosymbionts. More than 120 samples were examined also by means of PCR using trichomonad-specific primers. We determined sequences of SSU rDNA and ITS region of two isolates of the genus Simplicimonas Cepicka, Hampl et Kulda, 2010 and one isolate of Monocercomonas colubrorum (Hammerschmidt, 1844). Although our results showed that trichomonads are very rare inhabitants of the intestine of true bugs, two of three isolated flagellates belong to species specific for reptiles. The possibility of transmission of trichomonads between reptiles and true bugs is discussed.
authors: Pavla
authors: P
authors: Smejkalová
authors: Jan
authors: J
authors: Votýpka
authors: Julius
authors: J
authors: Lukes
authors: Ivan
authors: I
authors: Cepicka
mesh_terms: D000818:Animals; D016054:DNA, Protozoan; D020062:Heteroptera / Q000469:parasitology*; D006790:Host-Parasite Interactions; D013045:Species Specificity; D016834:Trichomonadida / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D016054:DNA, Protozoan