title: Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland.
abstract: Over the last decades, Candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. Knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. The aim of this study was to evaluate the genetic diversity of 31 Candida albicans and 17 Candida glabrata isolates recovered from intensive care unit patients from the tertiary hospital in Krakow between 2011-2012. The strains were typed by random amplified polymorphic DNA (RAPD) polymerase chain reaction using five primers (CD16AS, HP1247, ERIC-2, OPE-3 and OPE-18). The results of the present investigation revealed a high degree of genetic diversity among the isolates. No clonal relationship was found among the C. albicans strains, whereas two C. glabrata isolates were identical. The source of Candida infection appeared to be mostly endogenous; however, the presence of two clonal C. glabrata strains suggested the possibility of cross-transmission of these pathogens. Our study confirmed the high discriminatory power of the RAPD technique in the molecular typing of Candida clinical isolates. This method may be applied to the evaluation of transmission routes of pathogenic fungi on a local level.
authors: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
authors: Paulina
authors: P
authors: Paluchowska
authors: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
authors: Marianna
authors: M
authors: Tokarczyk
authors: Microbiological Laboratory, Department of Laboratory Diagnostics, Rydygier?s Specialized Hospital, Krakow, Poland.
authors: Bozena
authors: B
authors: Bogusz
authors: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
authors: Iwona
authors: I
authors: Skiba
authors: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
authors: Alicja
authors: A
authors: Budak
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000935:Antifungal Agents / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects / Q000235:genetics*; D041221:Candida glabrata / Q000187:drug effects / Q000235:genetics*; D002177:Candidiasis / Q000453:epidemiology* / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005260:Female; D006801:Humans; D007362:Intensive Care Units; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D058889:Molecular Typing; D011044:Poland / Q000453:epidemiology; D019105:Random Amplified Polymorphic DNA Technique; D055815:Young Adult
chemical_list: D000935:Antifungal Agents; D017931:DNA Primers; D004271:DNA, Fungal