title: Genome-wide association analysis to identify SNP markers affecting teat numbers in an F2 intercross population between Landrace and Korean native pigs.
abstract: Most reproductive traits have low heritability and are greatly affected by environmental factors. Teat number and litter size are traits related to the reproduction ability of pigs. To identify quantitative trait loci (QTLs) for teat number traits, a genome-wide association study (GWAS) was conducted using an F2 intercross between Landrace and Korean native pigs. Genotype analysis was performed using the porcine SNP 60 K beadchip. The GWAS was performed using a mixed-effects model and linear regression approach. When a genome-wide threshold was determined using the Bonferroni method (P = 1.61 × 10(-6)), 38 single nucleotide polymorphism (SNP) markers in pig chromosome 7 (SSC7) were significantly associated with three teat number traits (total teat number, left teat number, and right teat number). Among these, SNPs in 5 genes (HDDC3, LOC100156276, LOC100155863, ANPEP, SCAMP2) were selected for further study based primarily on their statistical significance. A significant association was detected in SCAMP2 g.25280 G>A for total teat number (P = 2.0 × 10(-12)), HDDC3 g.1319 G>A SNP for left teat number (P = 2.3 × 10(-7)), and SCAMP2 g.14198 G>A for right teat number (P = 4.7 × 10(-12)). These results provide valuable information about the selective breeding for desirable teat numbers in pigs.
authors: Division of Applied Life Science, Graduate School of Gyeongsang National University, Jinju, 660-701, Korea, bilf@gnu.ac.kr.
authors: Jae-Bong
authors: JB
authors: Lee
authors: Eun-Ji
authors: EJ
authors: Jung
authors: Hee-Bok
authors: HB
authors: Park
authors: Shil
authors: S
authors: Jin
authors: Dong-Won
authors: DW
authors: Seo
authors: Moon-Suck
authors: MS
authors: Ko
authors: In-Cheol
authors: IC
authors: Cho
authors: Jun-Heon
authors: JH
authors: Lee
authors: Hyun-Tae
authors: HT
authors: Lim
mesh_terms: D000818:Animals; D001947:Breeding / Q000379:methods*; D003433:Crosses, Genetic; D005260:Female; D055106:Genome-Wide Association Study; D006824:Hybridization, Genetic / Q000235:genetics*; D016014:Linear Models; D008321:Mammary Glands, Animal / Q000033:anatomy & histology*; D008957:Models, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D056910:Republic of Korea; D034421:Sus scrofa / Q000033:anatomy & histology / Q000235:genetics*