title: Characterization of Bbx, a member of a novel subfamily of the HMG-box superfamily together with Cic.
abstract: High mobility group (HMG)-box proteins, a large and functionally diverse superfamily of architectural protein, are involved in the regulation of DNA-dependent processes such as transcription, replication and DNA repair via the HMG-box domain. Bobby sox homolog (BBX), a newly identified HMG-Box protein, may function as a sequence-specific transcription factor. However, its expression pattern and biological functions are largely unknown. In this work, phylogenetic analysis showed that BBX is highly conserved and belongs to a novel subfamily of HMG-Box superfamily together with CIC (capicua homolog). Real time RT-PCR and whole-mount in situ hybridization in zebrafish embryo revealed that bbx, cica, and cicb were maternally highly expressed from 4 cell to 1K cell stage, and the zygote expression was primarily distributed in the central nervous system (CNS) from 24 to 60 h post-fertilization (hpf). Immunohistochemistry analysis in mouse brain revealed that BBX was weakly expressed in the cerebellum and highly expressed in the cortex and hippocampus. These findings indicate that as a novel HMG-box protein, BBX maybe associated with CNS development and provides useful clues to further study of its biological functions.
authors: Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China, chentielin2005@126.com.
authors: TieLin
authors: T
authors: Chen
authors: Li
authors: L
authors: Zhou
authors: Yue
authors: Y
authors: Yuan
authors: Yin
authors: Y
authors: Fang
authors: Yue
authors: Y
authors: Guo
authors: HuiZhe
authors: H
authors: Huang
authors: Qin
authors: Q
authors: Zhou
authors: XiaoYan
authors: X
authors: Lv
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015870:Gene Expression; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: C000594039:BBX protein, mouse; C000594040:Bbx protein, zebrafish; D006609:High Mobility Group Proteins; D014157:Transcription Factors