title: Low and high doses of UV-B differentially modulate chlorpyrifos-induced alterations in nitrogen metabolism of cyanobacteria.
abstract: The present study assessed the comparative responses on the specific growth rate, nitrogen metabolism and enzymes associated with nitrogen metabolism in two nitrogen fixing cyanobacteria-Nostoc muscorum and Phormidium foveolarum exposed to two UV-B doses (low; UV-BL: 0.5472kJm(-2) and high; UV-BH: 5.472kJm(-2)) and two doses of the insecticide chlorpyrifos (O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphorothioate; low i.e. CPL, 1µgml(-1) and high i.e. CPH, 2µgml(-1)) singly and in combination. The specific growth rate, NO3(-) and NO2(-) uptake, nitrate assimilating enzymes - nitrate reductase and nitrite reductase and ammonium assimilating enzymes - glutamine synthetase and glutamate synthase were severely affected when treated either with CPH or/and UV-BH while glutamate dehydrogenase exhibited a stimulatory response. CPL also reduced all the measured parameters (except GDH activity) after 24h, however, a stimulatory effect was observed after 72h due to an increase in nitrogen metabolism (and other antioxidant) enzymes during this period. UV-BL did not cause significant alteration in the studied parameters while in combination with CP doses, it either alleviated the inhibitory effects or further enhanced the CPL induced activities of these enzymes (except GDH). Overall results indicate the resistant nature of P. foveolarum against the inhibitory doses of UV-B and chlorpyrifos in comparison to N. muscorum.
authors: Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad (A Central University of India), Allahabad-211 002, India. Electronic address: prabhatsrivastava.au@gmail.com.
authors: Prabhat Kumar
authors: PK
authors: Srivastava
authors: Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad (A Central University of India), Allahabad-211 002, India. Electronic address: vijaypratap.au@gmail.com.
authors: Vijay Pratap
authors: VP
authors: Singh
authors: Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad (A Central University of India), Allahabad-211 002, India. Electronic address: profsmprasad@gmail.com.
authors: Sheo Mohan
authors: SM
authors: Prasad
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D004390:Chlorpyrifos / Q000008:administration & dosage / Q000633:toxicity*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005970:Glutamate Synthase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D050901:Nitrate Reductase / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D050502:Nostoc muscorum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D007306:Insecticides; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; D050901:Nitrate Reductase; D005974:Glutamate-Ammonia Ligase; D004390:Chlorpyrifos; D009584:Nitrogen
keywords: Chlorpyrifos; Cyanobacteria; High UV-B dose; Low UV-B dose; Nitrogen metabolism