title: Poly(NaSS) functionalization modulates the conformation of fibronectin and collagen type I to enhance osteoblastic cell attachment onto Ti6Al4V.
abstract: Functionalization of surfaces with poly(sodium styrenesulfonate) (poly(NaSS)) has recently been found to enhance osteointegration of implantable materials. Radical polymerization of poly(NaSS) on titanium (Ti)-based substrates has been used to improve their long-term performance by preventing fibrosis and consequently implant loosening. However, the influence of the sulfonate groups on the early cell behavior and the associated molecular phenomena remains to be understood. In this work, we used quartz crystal microbalance with dissipation (QCM-D) to elucidate the role of poly(NaSS) in enhancing osteoblastic cell attachment. This was measured by following the cell attachment using the MC3T3-E1 cell line, on fetal bovine serum (FBS) preadsorbed surfaces and on substrates adsorbed with a series of relevant proteins, bovine serum albumin (BSA), fibronectin (Fn), and collagen type I (Col I). Comparison of the performance of poly(NaSS) with other clinically important substrates such as Ti alloy Ti6Al4V, gold, and poly(desamino-tyrosyl-tyrosine ethyl ester carbonate) (poly(DTEc)) indicates poly(NaSS) to be a superior substrate for MC3T3-E1 cells attachment. This attachment was found to be integrin mediated in the presence of Fn and Col I. Antibodies specific to the RGD peptide and the N- and C-terminal HB-binding domains reacted more intensively with Fn adsorbed on poly(NaSS). Fn adapts a conformation favorable to RGD mediated cell attachment when adsorbed onto poly(NaSS).
authors: Laboratory of Biomaterials and Specialty Polymers, LBPS-CSPBAT CNRS UMR 7244, Institut Galilée, Université Paris 13 , 93430 Villetaneuse, France.
authors: Helena P
authors: HP
authors: Felgueiras
authors: Sven D
authors: SD
authors: Sommerfeld
authors: N Sanjeeva
authors: NS
authors: Murthy
authors: Joachim
authors: J
authors: Kohn
authors: Véronique
authors: V
authors: Migonney
mesh_terms: D016475:3T3 Cells; D000535:Aluminum / Q000737:chemistry; D000818:Animals; D001704:Biopolymers / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D051379:Mice; D008968:Molecular Conformation; D010006:Osteoblasts / Q000166:cytology*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry; D014639:Vanadium / Q000737:chemistry
chemical_list: D001704:Biopolymers; D024042:Collagen Type I; D005353:Fibronectins; D011137:Polystyrenes; C505373:poly(desaminotyrosyl-tyrosine-ethyl ester carbonate); D014639:Vanadium; D012710:Serum Albumin, Bovine; D014443:Tyrosine; C003321:polystyrene sulfonic acid; D006046:Gold; D000535:Aluminum; D014025:Titanium