title: Simulated moving bed purification of flaxseed oil orbitides: unprecedented separation of cyclolinopeptides C and E.
abstract: The purification and enrichment of most natural products with potential pharmaceutical applications has been performed mainly employing conventional batch-mode chromatographic processes. There is a growing interest in use of simulated moving bed (SMB) chromatography for natural product enrichment as this method enables conservation of mobile phase, while increasing productivity of chromatography medium. SMB increases yield while decreasing cost. Cyclolinopeptides C ([1-9-NaC],[1-MetO]-CLB, 3) and E ([1-8-NaC],[1-MetO]-CLE, 8) were extracted as a mixture from flaxseed oil and then enriched using a three-zone simulated moving bed. The current research extends the SMB technology to enrichment of cyclolinopeptides (CLs), a group of biologically active hydrophobic cyclic peptides that occur in flaxseed oil. Of interest are [1-9-NaC],[1-MetO]-CLB (3) and [1-8-NaC],[1-MetO]-CLE (8) that provide synthetic scaffolds for modified CLs. The influence of flow rate (feed, desorbent, and extract) on the separation of [1-9-NaC],[1-MetO]-CLB (3) and [1-8-NaC],[1-MetO]-CLE (8) was investigated.
authors: Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada. Electronic address: denis.okinyo-owiti@usask.ca.
authors: Denis P
authors: DP
authors: Okinyo-Owiti
authors: Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada.
authors: Peta-Gaye G
authors: PG
authors: Burnett
authors: Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
authors: Martin J T
authors: MJ
authors: Reaney
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008043:Linseed Oil / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D010456:Peptides, Cyclic; C524751:cyclolinopeptide E; D008043:Linseed Oil
keywords: Cyclolinopeptide; Flow rate; Orbitide; Separation; Simulated moving bed chromatography