title: Terminal protection of small molecule-linked ssDNA for label-free and sensitive fluorescent detection of folate receptor.
abstract: In this work, based on terminal protection of folate-linked ssDNA (FA-ssDNA) and the SYBR Gold fluorescent dye, we describe the development of a label-free fluorescent strategy for the detection of folate receptors (FRs). The binding between the target FR and the FA moiety of the FA-ssDNA protects the FR bound FA-ssDNA from digesting by Exo I. The binding of SYBR Gold to the terminal protected, un-digested FA-ssDNA leads to enhanced fluorescent emission for the monitoring of FR with a detection limit of 30 pM. Besides, the developed method also shows high selectivity toward FR against other control proteins. Moreover, our approach avoids the labeling of the probes with fluorescent tags and achieves label-free detection of FR. With these advantages, the proposed method thus holds promising potential for the development of simple and convenient strategies for the detection of other proteins by using different small molecule receptor/protein ligand pairs.
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Yunying
authors: Y
authors: Xu
authors: School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, PR China. Electronic address: jiangby@cqut.edu.cn.
authors: Bingying
authors: B
authors: Jiang
authors: School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, PR China.
authors: Jiaqing
authors: J
authors: Xie
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: yunatswu@swu.edu.cn.
authors: Yun
authors: Y
authors: Xiang
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Ruo
authors: R
authors: Yuan
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Yaqin
authors: Y
authors: Chai
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D058973:Folate Receptors, GPI-Anchored / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D009930:Organic Chemicals / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D058973:Folate Receptors, GPI-Anchored; D009930:Organic Chemicals; C474470:SYBR Gold nucleic acid gel stain; D005492:Folic Acid
keywords: Fluorescence; Folate receptors; Folate-linked ssDNA; Terminal protection