title: PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: in vitro cell culture studies.
abstract: In the present study, we examined the potential of using highly porous poly(ε-caprolactone) (PCL)-coated hydroxyapatite (HAp) scaffold derived from cuttlefish bone for bone tissue engineering applications. The cell culture studies were performed in vitro with preosteoblastic MC3T3-E1 cells in static culture conditions. Comparisons were made with uncoated HAp scaffold. The attachment and spreading of preosteoblasts on scaffolds were observed by Live/Dead staining Kit. The cells grown on the HAp/PCL composite scaffold exhibited greater spreading than cells grown on the HAp scaffold. DNA quantification and scanning electron microscopy (SEM) confirmed a good proliferation of cells on the scaffolds. DNA content on the HAp/PCL scaffold was significantly higher compared to porous HAp scaffolds. The amount of collagen synthesis was determined using a hydroxyproline assay. The osteoblastic differentiation of the cells was evaluated by determining alkaline phosphatase (ALP) activity and collagen type I secretion. Furthermore, cell spreading and cell proliferation within scaffolds were observed using a fluorescence microscope.
authors: Faculty of Chemical Engineering and Technology, University of Zagreb, Croatia. Electronic address: dmilovac@fkit.hr.
authors: Dajana
authors: D
authors: Milovac
authors: Centre for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Spain.
authors: Tatiana C
authors: TC
authors: Gamboa-Martínez
authors: Faculty of Chemical Engineering and Technology, University of Zagreb, Croatia.
authors: Marica
authors: M
authors: Ivankovic
authors: Centre for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, Valencia, Spain.
authors: Gloria
authors: G
authors: Gallego Ferrer
authors: Faculty of Chemical Engineering and Technology, University of Zagreb, Croatia.
authors: Hrvoje
authors: H
authors: Ivankovic
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002460:Cell Line; D002468:Cell Physiological Phenomena / Q000187:drug effects; D003094:Collagen / Q000032:analysis; D049832:Decapodiformes; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D003094:Collagen; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: Cell differentiation; Cuttlefish bone; Hydroxyapatite scaffold; Poly(ε-caprolactone); Tissue engineering