title: A new chitinase-producer strain Streptomyces glauciniger WICC-A03: isolation and identification as a biocontrol agent for plants phytopathogenic fungi.
abstract: This study discusses the isolation and identification of a new Streptomycetes highly active chitinase producer. Fifteen strains were isolated from Malaysian soil samples. The isolate WICC-A03 was found to be the most active chitinase producer. Its antifungal activity was evaluated against many phytopathogens. The identification of WICC-A03 using phenotypic and genotypic methods strongly indicated that strain WICC-A03 belonged to the genus Streptomyces and displayed similarity (91%) with Streptomyces glauciniger. Thus, it was given the suggested name S. glauciniger WICC-A03 with accession number: JX139754. WICC-A03 produces extracellular chitinase in a medium containing 1.5% colloidal chitin in submerged culture on 144 h. The produced enzyme was partially characterised and its molecular weight of 50 kDa was determined by using SDS-PAGE. This study indicates that WICC-A03 is a potential chitinase producer for biocontrol of plant pathogens. Further experiments are being carried out to optimise medium composition and cultivation conditions under lab and bioreactor scale.
authors: a Chemistry of Natural and Microbial Product Department , Pharmaceutical Industry Division, National Research Centre , El-Bohouth St., Dokki, P.O.12311, Cairo , Egypt.
authors: Hassan Mohamed
authors: HM
authors: Awad
authors: Hesham Ali
authors: HA
authors: El-Enshasy
authors: Siti Zulaiha
authors: SZ
authors: Hanapi
authors: Eman Ramadan
authors: ER
authors: Hamed
authors: Bustanur
authors: B
authors: Rosidi
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification*; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism*; D005658:Fungi; D008296:Malaysia; D008826:Microbial Sensitivity Tests; D010935:Plant Diseases / Q000382:microbiology*; D012988:Soil Microbiology; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000935:Antifungal Agents; D002686:Chitin; D002688:Chitinases
keywords: Streptomyces glauciniger WICC-A03; antimicrobial activity; biocontrol; chitinase; isolation; phenotypic and genotypic identification