title: Inhibition of COX-2 and PGE2in LPS-stimulated RAW264.7 cells by lonimacranthoide VI, a chlorogenic acid ester saponin.
abstract: Lonimacranthoide VI, first isolated from the flower buds ofLonicera macranthoidesin our previous study, is a rare chlorogenic acid ester acylated at C-23 of hederagenin. In the present study, the anti-inflammatory effects of lonimacranthoide VI were studied. Lipopolysaccharides (LPS) induced an inflammatory response through the production of prostaglandin E2(PGE2), and these levels were reduced when lonimacranthoide VI was pre-administered. Additionally, the mechanism of the anti-inflammatory effects of lonimacranthoide VI was investigated by measuring cyclooxygenase (COX) activity and mRNA expression. The results showed that lonimacranthoide VI inhibited mRNA expression andin vitroactivity ofCOX-2in a dose-dependent manner, whereas only the higher lonimacranthoide VI concentration possibly reducedCOX-1expression andin vitroactivity. Taken together, these results indicate that lonimacranthoide VI is an important anti-inflammatory constituent ofLonicera macranthoidesand that the anti-inflammatory effect is attributed to the inhibition of PGE2production through COX activity and mRNA expression.
authors: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences/Nanjing Botanical Garden Memorial Sun Yat-Sen, Nanjing, Jiangsu 210014, P.R. China ; College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China.
authors: Fuqin
authors: F
authors: Guan
authors: College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China.
authors: Haiting
authors: H
authors: Wang
authors: College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China.
authors: Yu
authors: Y
authors: Shan
authors: College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China.
authors: Yu
authors: Y
authors: Chen
authors: College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China.
authors: Ming
authors: M
authors: Wang
authors: College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China.
authors: Qizhi
authors: Q
authors: Wang
authors: College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China.
authors: Min
authors: M
authors: Yin
authors: College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China.
authors: Youyi
authors: Y
authors: Zhao
authors: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences/Nanjing Botanical Garden Memorial Sun Yat-Sen, Nanjing, Jiangsu 210014, P.R. China ; College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China.
authors: Xu
authors: X
authors: Feng
authors: Jiangsu Key Laboratory for Bioresources of Saline Soils, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences/Nanjing Botanical Garden Memorial Sun Yat-Sen, Nanjing, Jiangsu 210014, P.R. China.
authors: Jianhua
authors: J
authors: Zhang
keywords: Lonicera macranthoides; cyclooxygenase-1; cyclooxygenase-2; lonimacranthoide VI; prostaglandin E2