title: Phloroglucinol induces apoptosis via apoptotic signaling pathways in HT-29 colon cancer cells.
abstract: Phloroglucinol is a polyphenolic compound that is used to treat and prevent several human diseases, as it exerts beneficial biological activities, including anti-oxidant, anti‑inflammatory and anticancer properties. The aim of the present study was to investigate the effects of phloroglucinol on apoptotic signaling pathways in HT-29 colon cancer cells. The results indicated that phloroglucinol suppressed cell viability and induced apoptosis in HT-29 cells in a concentration-dependent manner. Phloroglucinol treatment of HT-29 cells resulted in characteristic apoptosis-related changes: altered Bcl-2 family proteins, cytochrome c release, and activation of caspase-3 and caspase-8. This study also showed that proteins involved in apoptosis were stimulated by treatment with phloroglucinol. These findings demonstrated that phloroglucinol exerts anticancer activity in HT-29 colon cancer cells through induction of apoptosis.
authors: Department of Food and Life Science, Pukyong National University, Nam-gu, Busan 608-737, Republic of Korea.
authors: Mi-Hye
authors: MH
authors: Kang
authors: Department of Food and Life Science, Pukyong National University, Nam-gu, Busan 608-737, Republic of Korea.
authors: In-Hye
authors: IH
authors: Kim
authors: Department of Food and Life Science, Pukyong National University, Nam-gu, Busan 608-737, Republic of Korea.
authors: Taek-Jeong
authors: TJ
authors: Nam
mesh_terms: D000230:Adenocarcinoma; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000187:drug effects; D053181:Caspase 8 / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms; D045304:Cytochromes c / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D007421:Intestine, Small / Q000166:cytology; D010696:Phloroglucinol / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: C000595707:BCL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D045304:Cytochromes c; D010696:Phloroglucinol; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8