title: Simulation of metabolism-based herb-drug interaction: towards safe and efficacious use of NIPRD-AM1.
abstract: OBJECTIVE
  To evaluate the effect of NIPRD-AM1 on CYP3A4 in order to generate clinically significant data for its safe and efficacious use.
  
  
  METHODS
  NIPRD-AM1 is a phytomedicine developed from aqueous root extracts of Nauclea latifolia Smith (Rubiaceae) for the treatment of uncomplicated malaria. The effect of NIPRD-AM1 on CYP3A4 was measured with and without the addition of NIPRD-AM1, by testing different concentrations of the product at 37 °C in reactive mixtures with ketoconazole (2.5 µM) as the positive control.
  
  
  RESULTS
  RESULTS showed a very low IC50 value of 0.01 mg/ml similar to that of ketoconazole (0.016 mg/ml).
  
  
  CONCLUSIONS
  Metabolic processes of NIPRD-AM1 are likely to inhibit CYP3A4, with potential implication on drugs that are CYP3A4 substrates. This is a promising approach for guidance towards the safe and efficacious use of NIPRD-AM1.
authors: Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development, PMB 21, Abuja, Nigeria.
authors: Bulus
authors: B
authors: Adzu
authors: Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development, PMB 21, Abuja, Nigeria.
authors: Kudirat Bola
authors: KB
authors: Mustapha
authors: African Institute of Biomedical Science and Technology (AiBST), Cnr Chinhoyi Str./Jason Moyo Ave. No. 9 at LAPF Centre, Harare, Zimbabwe.
authors: Collen
authors: C
authors: Masimirembwa
authors: Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development, PMB 21, Abuja, Nigeria.
authors: Obiageri
authors: O
authors: Obodozie
authors: Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development, PMB 21, Abuja, Nigeria.
authors: Rukaiyatu Abdullahi
authors: RA
authors: Kirim
authors: Director General/Chief Executive Officer, National Institute for Pharmaceutical Research and Development, PMB 21, Abuja, Nigeria.
authors: Karniyus Shingu
authors: KS
authors: Gamaniel
keywords: CYP3A4; Herb-Drug Interactions; NIPRD-AM1; Nauclea latifolia