title: An infectious bat-derived chimeric influenza virus harbouring the entry machinery of an influenza A virus.
abstract: In 2012, the complete genomic sequence of a new and potentially harmful influenza A-like virus from bats (H17N10) was identified. However, infectious influenza virus was neither isolated from infected bats nor reconstituted, impeding further characterization of this virus. Here we show the generation of an infectious chimeric virus containing six out of the eight bat virus genes, with the remaining two genes encoding the haemagglutinin and neuraminidase proteins of a prototypic influenza A virus. This engineered virus replicates well in a broad range of mammalian cell cultures, human primary airway epithelial cells and mice, but poorly in avian cells and chicken embryos without further adaptation. Importantly, the bat chimeric virus is unable to reassort with other influenza A viruses. Although our data do not exclude the possibility of zoonotic transmission of bat influenza viruses into the human population, they indicate that multiple barriers exist that makes this an unlikely event.
authors: 1] Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany [2].
authors: Mindaugas
authors: M
authors: Juozapaitis
authors: 1] Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany [2] Spemann Graduate School of Biology and Medicine, University of Freiburg, 79104 Freiburg, Germany [3] Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany [4].
authors: Étori
authors: É
authors: Aguiar Moreira
authors: 1] Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA [2] Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
authors: Ignacio
authors: I
authors: Mena
authors: 1] Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany [2] Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.
authors: Sebastian
authors: S
authors: Giese
authors: Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany.
authors: David
authors: D
authors: Riegger
authors: Institute of Diagnostic Virology, Friedrich-Loeffler-Institute, 17493 Greifswald-Insel Riems, Germany.
authors: Anne
authors: A
authors: Pohlmann
authors: Institute of Diagnostic Virology, Friedrich-Loeffler-Institute, 17493 Greifswald-Insel Riems, Germany.
authors: Dirk
authors: D
authors: Höper
authors: Institute of Virology and Immunology (IVI), 3147 Mittelhäusern, Switzerland.
authors: Gert
authors: G
authors: Zimmer
authors: Institute of Diagnostic Virology, Friedrich-Loeffler-Institute, 17493 Greifswald-Insel Riems, Germany.
authors: Martin
authors: M
authors: Beer
authors: 1] Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA [2] Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA [3] Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
authors: Adolfo
authors: A
authors: García-Sastre
authors: Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany.
authors: Martin
authors: M
authors: Schwemmle
mesh_terms: D000547:Amantadine / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002642:Chick Embryo; D002645:Chickens; D002678:Chimera / Q000235:genetics* / Q000502:physiology; D002685:Chiroptera / Q000821:virology*; D004285:Dogs; D024882:Drug Resistance, Viral / Q000235:genetics / Q000502:physiology; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009975:Orthomyxoviridae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D013552:Swine; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology; D053586:Virus Internalization; D015047:Zoonoses / Q000635:transmission
chemical_list: D000998:Antiviral Agents; D014764:Viral Proteins; D000547:Amantadine