title: In situ hybridization (both radioactive and nonradioactive) and spatiotemporal gene expression analysis.
abstract: Section in situ hybridization using either radioactive or nonradioactive labeled cDNA probes is an invaluable technique that enables the investigator to detect and localize mRNA expression within tissue sections and cells. Here, we describe the labeling of (35)S-UTP radioactive and nonradioactive digoxigenin probes, preparation of tissue sections, hybridization, and washing of non-hybridized probes, followed by the detection of radioactive signals via dipping in nuclear emulsion and the immunohistochemical and subsequent colorimetric detection of nonradioactive signals.
authors: HB Wells Center for Pediatric Research, Indiana University School of Medicine, 1044 West Walnut Street, Room R4 W402E, Indianapolis, IN, 46202, USA.
authors: Olga
authors: O
authors: Simmons
authors: Esther M
authors: EM
authors: Bolanis
authors: Jian
authors: J
authors: Wang
authors: Simon J
authors: SJ
authors: Conway
mesh_terms: D000818:Animals; D001345:Autoradiography; D004076:Digoxigenin / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D015347:RNA Probes / Q000235:genetics / Q000378:metabolism; D062211:Spatio-Temporal Analysis; D013462:Sulfur Radioisotopes
chemical_list: D015347:RNA Probes; D013462:Sulfur Radioisotopes; D004076:Digoxigenin