title: A new bioadhesive material from fish parasite Neobenedenia girellae.
abstract: The purpose of the work was to identify the proteins present in the adhesive material of the capsalid Neobenedenia girellae by a proteomic approach based on de novo sequencing and data base search to overcome the lack of information concerning the genome of these parasites. Glandular secretions were obtained by a new method, set up in our laboratory, which allowed collecting a small amount of secretion without any contamination from other tissues either from the parasites as well as from the skin of the host. The proteomic analysis reveals that the adhesive is mainly composed of cytoskeletal proteins (actin, keratin and tubulin) but contains also ATP-synthase, 78 kDa glucose regulated protein and albumin.
  
  
  CONCLUSIONS
  This paper reports for the first time the characterization of a novel bioadhesive material used by capsalid parasites to adhere to fish. Such information broadens our knowledge of the molecular mechanisms involved in adhesiveness of parasites to hosts. Moreover, it offers new clues in understanding the mechanism of stickiness and adhesion of cytoskeleton components, often involved in both physiological and pathological processes, including neurodegenerative diseases.
authors: Department of Veterinary Science and Public Health, Università degli Studi di Milano, via Celoria 10, 20133 Milano, Italy; Fondazione Filarete, viale Ortles 22/4, 20139 Milano, Italy.
authors: Elisa
authors: E
authors: Maffioli
authors: Department of Veterinary Science and Public Health, Università degli Studi di Milano, via Celoria 10, 20133 Milano, Italy; Fondazione Filarete, viale Ortles 22/4, 20139 Milano, Italy.
authors: Simona
authors: S
authors: Nonnis
authors: Department of Veterinary Science and Public Health, Università degli Studi di Milano, via Celoria 10, 20133 Milano, Italy; Fondazione Filarete, viale Ortles 22/4, 20139 Milano, Italy.
authors: Nerea Cuevas
authors: NC
authors: Polo
authors: Department of Veterinary Science and Public Health, Università degli Studi di Milano, via Celoria 10, 20133 Milano, Italy; Fondazione Filarete, viale Ortles 22/4, 20139 Milano, Italy.
authors: Armando
authors: A
authors: Negri
authors: Department of Biotechnology and Biosciences, Università di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors: Matilde
authors: M
authors: Forcella
authors: Department of Biotechnology and Biosciences, Università di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors: Paola
authors: P
authors: Fusi
authors: Department of Biotechnology and Biosciences, Università di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors: Paolo
authors: P
authors: Galli
authors: Department of Veterinary Science and Public Health, Università degli Studi di Milano, via Celoria 10, 20133 Milano, Italy; Fondazione Filarete, viale Ortles 22/4, 20139 Milano, Italy. Electronic address: gabriella.tedeschi@unimi.it.
authors: Gabriella
authors: G
authors: Tedeschi
mesh_terms: D025181:ATP Synthetase Complexes / Q000737:chemistry*; D000269:Adhesives / Q000032:analysis / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001688:Biological Products / Q000032:analysis / Q000737:chemistry*; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005399:Fishes / Q000469:parasitology*; D006360:Heat-Shock Proteins / Q000032:analysis / Q000737:chemistry*; D008422:Materials Testing; D008969:Molecular Sequence Data; D010986:Platyhelminths / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods
chemical_list: D000269:Adhesives; D001688:Biological Products; D003598:Cytoskeletal Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D025181:ATP Synthetase Complexes
keywords: Adhesive; Capsalid; De novo MS/MS sequencing; Natural glue; Neobenedenia girellae