title: Finding the striatum in sheep: use of a multi-modal guided approach for convection enhanced delivery.
abstract: Our goal is delivery of a long-term treatment for Huntington's disease. We administer intracerebrally in sheep adeno-associated virus (AAV) to establish optimal safety, spread and neuronal uptake of AAV based therapeutics. Sheep have large gyrencephalic brains and offer the opportunity to study a transgenic Huntington's disease model. However, lack of a relevant brain stereotactic atlas and the difficulty of skull fixation make conventional stereotaxy unreliable. We describe a multi-modal image-guidance technique to achieve accurate placement of therapeutics into the sheep striatum.
authors: Department of Radiology, University of Massachusetts, Worcester, MA, USA.
authors: I M J
authors: IM
authors: van der Bom
authors: Department of Neurosurgery, University of Massachusetts, Worcester, MA, USA.
authors: R P
authors: RP
authors: Moser
authors: Departments of Microbiology & Physiological Systems, Immunology & Virology, Molecular Genetics & Microbiology, Gene Therapy Center, and RNA Therapeutics Institute, University of Massachusetts, Worcester, MA, USA.
authors: G
authors: G
authors: Gao
authors: Department of Medicine, Neuro-therapeutics Institute and RNA Therapeutics Institute, University of Massachusetts, Worcester, MA, USA.
authors: E
authors: E
authors: Mondo
authors: Department of Animal Medicine, University of Massachusetts, Worcester, MA, USA.
authors: D
authors: D
authors: O'Connell
authors: Department of Radiology, University of Massachusetts, Worcester, MA, USA.
authors: M J
authors: MJ
authors: Gounis
authors: Departments of Microbiology & Physiological Systems, Immunology & Virology, Molecular Genetics & Microbiology, Gene Therapy Center, and RNA Therapeutics Institute, University of Massachusetts, Worcester, MA, USA.
authors: S
authors: S
authors: McGowan
authors: Departments of Microbiology & Physiological Systems, Immunology & Virology, Molecular Genetics & Microbiology, Gene Therapy Center, and RNA Therapeutics Institute, University of Massachusetts, Worcester, MA, USA.
authors: J
authors: J
authors: Chaurette
authors: Department of Animal Medicine, University of Massachusetts, Worcester, MA, USA.
authors: N
authors: N
authors: Bishop
authors: Department of Neurology, University of Massachusetts, Worcester, MA, USA.
authors: M S
authors: MS
authors: Sena-Esteves
authors: Departments of Pediatrics and Gene Therapy Center, University of Massachusetts, Worcester, MA, USA.
authors: C
authors: C
authors: Mueller
authors: Departments of Microbiology & Physiological Systems, Immunology & Virology, Molecular Genetics & Microbiology, Gene Therapy Center, and RNA Therapeutics Institute, University of Massachusetts, Worcester, MA, USA.
authors: N
authors: N
authors: Aronin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D018481:Convection; D003342:Corpus Striatum / Q000033:anatomy & histology / Q000601:surgery*; D000229:Dependovirus; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage*; D006816:Huntington Disease / Q000628:therapy; D034561:Sheep, Domestic; D013238:Stereotaxic Techniques
keywords: Sheep brain; huntingtin therapeutics; stereotaxis placement