title: Effects of calcium silicate endodontic cements on biocompatibility and mineralization-inducing potentials in human dental pulp cells.
abstract: BACKGROUND
  The objective of this study was to evaluate the biocompatibility, inflammatory response, and odontoblastic potential of Biodentine (Septodont, Saint Maur des Fosses, France), Ortho-MTA (OMTA; BioMTA, Seoul, Korea), Angelus-MTA (AMTA; Angelus, Londrina, Brazil), and IRM (Dentsply Tulsa Dental, Tulsa, OK) in human dental pulp cells. The underlying signaling mechanisms were also investigated.
  
  
  METHODS
  Biocompatibilities were examined by the 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide assay. Differentiation was assessed by alkaline phosphatase activity, alizarin red S staining, and reverse-transcription polymerase chain reaction for marker genes. The levels of inflammatory mediators and cytokines were measured by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Signal transduction analysis was performed by Western blotting.
  
  
  RESULTS
  Biodentine, OMTA, and AMTA showed favorable cell proliferation, alkaline phosphatase activity, formation of mineralized nodules, and expression of odontoblastic marker genes that were similar to those of IRM. The levels of proinflammatory mediators including nitric oxide, prostaglandin E2, inducible nitric oxide synthase, and cyclooxygenase-2 were lower for Biodentine, OMTA, and AMTA compared with the IRM group. All test materials induced reactive oxygen species production and the expression of hemeoxygenase-1, nuclear factor-E2-related factor-2, and mitogen-activated protein kinases.
  
  
  CONCLUSIONS
  These data indicate for the first time that the biocompatibility, inflammatory response, and odontoblastic differentiation of Biodentine were similar to that of OMTA and AMTA in HDPCs, which suggests that Biodentine could be good alternative pulp capping agent.
authors: Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors: Seok-Woo
authors: SW
authors: Chang
authors: Maxillofacial Tissue Regeneration and Research Center for Tooth and Periodontal Regeneration, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors: So-Youn
authors: SY
authors: Lee
authors: Pediatric Dentistry and School of Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors: Hyo-Jung
authors: HJ
authors: Ann
authors: Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea; Department of Conservative Dentistry, Dental Research Institute and BK 21 Program, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors: Kee-Yeon
authors: KY
authors: Kum
authors: Maxillofacial Tissue Regeneration and Research Center for Tooth and Periodontal Regeneration, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea. Electronic address: eckim@khu.ac.kr.
authors: Eun-Cheol
authors: EC
authors: Kim
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D017607:Aluminum Compounds / Q000494:pharmacology; D001672:Biocompatible Materials / Q000494:pharmacology*; D001729:Bismuth / Q000494:pharmacology; D002113:Calcification, Physiologic / Q000187:drug effects*; D017610:Calcium Compounds / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D003738:Dental Cements / Q000494:pharmacology; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D015232:Dinoprostone / Q000032:analysis; D004338:Drug Combinations; D051547:Heme Oxygenase-1 / Q000032:analysis; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D008422:Materials Testing; D008768:Methylmethacrylates / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000032:analysis; D051267:NF-E2-Related Factor 2 / Q000032:analysis; D009569:Nitric Oxide / Q000032:analysis; D052247:Nitric Oxide Synthase Type II / Q000032:analysis; D009804:Odontoblasts / Q000187:drug effects; D010087:Oxides / Q000494:pharmacology; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis; D012387:Root Canal Filling Materials / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D017640:Silicates / Q000494:pharmacology*; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D016207:Cytokines; D003738:Dental Cements; D004338:Drug Combinations; D018836:Inflammation Mediators; C525641:MTA Bio; C475480:MTA-Angelus; D008768:Methylmethacrylates; D051267:NF-E2-Related Factor 2; D010087:Oxides; D057872:Pulp Capping and Pulpectomy Agents; D017382:Reactive Oxygen Species; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C086631:mineral trioxide aggregate; D009569:Nitric Oxide; C506393:tricalcium silicate; C031667:IRM cement; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D051547:Heme Oxygenase-1; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D020928:Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase; D015232:Dinoprostone; C031293:calcium silicate; D001729:Bismuth
keywords: Biocompatibility; Biodentine; dental pulp cells; heme oxygenase-1; inflammatory response; nuclear factor–E2-related factor-2; odontoblastic potential