title: Evidence of a conserved role for Chlamydia HtrA in the replication phase of the chlamydial developmental cycle.
abstract: Identification of the HtrA inhibitor JO146 previously enabled us to demonstrate an essential function for HtrA during the mid-replicative phase of the Chlamydia trachomatis developmental cycle. Here we extend our investigations to other members of the Chlamydia genus. C. trachomatis isolates with distinct replicative phase growth kinetics showed significant loss of viable infectious progeny after HtrA was inhibited during the replicative phase. Mid-replicative phase addition of JO146 was also significantly detrimental to Chlamydia pecorum, Chlamydia suis and Chlamydia cavie. These data combined indicate that HtrA has a conserved critical role during the replicative phase of the chlamydial developmental cycle.
authors: Institute of Health and Biomedical Innovation and School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia.
authors: Pooja
authors: P
authors: Patel
authors: Institute of Health and Biomedical Innovation and School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia.
authors: Leonore
authors: L
authors: De Boer
authors: Faculty of Science, Health, Education, and Engineering, University of the Sunshine Coast, Maroochydore DC, QLD 4558, Australia.
authors: Peter
authors: P
authors: Timms
authors: Institute of Health and Biomedical Innovation and School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia. Electronic address: w.huston@qut.edu.au.
authors: Wilhelmina May
authors: WM
authors: Huston
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002689:Chlamydia / Q000201:enzymology* / Q000254:growth & development*; D050296:Microbial Viability / Q000187:drug effects; D011480:Protease Inhibitors / Q000378:metabolism; D057057:Serine Proteases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011480:Protease Inhibitors; D057057:Serine Proteases
keywords: Chlamydia; HtrA; Inhibitor; Protease