title: Osteogenesis and angiogenesis properties of dental pulp cell on novel injectable tricalcium phosphate cement by silica doped.
abstract: β-Tricalcium phosphate (β-TCP) is an osteoconductive material in clinical. In this study, we have doped silica (Si) into β-TCP and enhanced its bioactive and osteostimulative properties. To check its effectiveness, a series of Si-doped with different ratios were prepared to make new bioactive and biodegradable biocomposites for bone repair. Formation of the diametral tensile strength, ions released and weight loss of cements was considered after immersion. In addition, we also examined the behavior of human dental pulp cells (hDPCs) cultured on Si-doped β-TCP cements. The results showed that setting time and injectability of the Si-doped β-TCP cements were decreased as the Si content was increased. At the end of the immersion point, weight losses of 30.1%, 36.9%, 48.1%, and 55.3% were observed for the cement doping 0%, 10%, 20%, and 30% Si into β-TCP cements, respectively. In vitro cell experiments show that the Si-rich cements promote human dental pulp cell (hDPC) proliferation and differentiation. However, when the Si-doped in the cement is more than 20%, the amount of cells and osteogenesis protein of hDPCs was stimulated by Si released from Si-doped β-TCP cements. The degradation of β-TCP and osteogenesis of Si gives a strong reason to believe that these Si-doped β-TCP cements may prove to be promising bone repair materials.
authors: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Stomatology, Chung Shan Medical University Hospital, Taichung, Taiwan; School of Dentistry, Chung Shan Medical University, Taichung, Taiwan.
authors: Ying-Fang
authors: YF
authors: Su
authors: Department of Anatomy, Chung Shan Medical University, Taichung City, Taiwan. Electronic address: chichang31@gmail.com.
authors: Chi-Chang
authors: CC
authors: Lin
authors: Department of Stomatology, Chung Shan Medical University Hospital, Taichung, Taiwan; School of Dentistry, Chung Shan Medical University, Taichung, Taiwan.
authors: Tsui-Hsien
authors: TH
authors: Huang
authors: Department of Stomatology, Chung Shan Medical University Hospital, Taichung, Taiwan; School of Dentistry, Chung Shan Medical University, Taichung, Taiwan.
authors: Ming-Yung
authors: MY
authors: Chou
authors: Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan. Electronic address: jjyang@csmu.edu.tw.
authors: Jaw-Ji
authors: JJ
authors: Yang
authors: Department of Anatomy, Chung Shan Medical University, Taichung City, Taiwan. Electronic address: eviltacasi@gmail.com.
authors: Ming-You
authors: MY
authors: Shie
mesh_terms: D001641:Bicuspid / Q000166:cytology; D002113:Calcification, Physiologic / Q000187:drug effects; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003738:Dental Cements / Q000737:chemistry / Q000494:pharmacology; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002130:Calcium Phosphates; D003738:Dental Cements; D012822:Silicon Dioxide; C018392:tricalcium phosphate
keywords: Angiogenic; Biodegradable; Injectability; Osteogenic; Silica-doped; β-Tricalcium phosphate