title: Highly sensitive detection of zearalenone in feed samples using competitive surface-enhanced Raman scattering immunoassay.
abstract: Accurate and quantitative analysis of mycotoxin (such as zearalenone) is particularly imperative in the field of food safety and animal husbandry. Here, we develop a sensitive and specific method for zearalenone detection using competitive surface-enhanced Raman scattering (SERS) immunoassay. In this assay, a functional gold nanoparticle was labeled with the Raman reporter and the zearalenone antibody, and a modified substrate was assembled with the zearalenone-bovine serum albumin. With the addition of free zearalenone, the competitive immune reaction between free zearalenone and zearalenone-bovine serum albumin was initiated for binding with zearalenone antibody labeled on gold nanoparticle, resulting in the change of SERS signal intensity. The proposed method exhibits high sensitivity with a detection limit of 1 pg/mL and a wide dynamic range from 1 to 1000 pg/mL. Furthermore, this method can be further applied to analyze the multiple natural feed samples contaminated with zearalenone, holding great potential for real sample detection.
authors: Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, People's Republic of China.
authors: Jianzhi
authors: J
authors: Liu
authors: Yongjun
authors: Y
authors: Hu
authors: Guichi
authors: G
authors: Zhu
authors: Xiaoming
authors: X
authors: Zhou
authors: Li
authors: L
authors: Jia
authors: Tao
authors: T
authors: Zhang
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*; D015025:Zearalenone / Q000032:analysis*
chemical_list: D015025:Zearalenone
keywords: Zearalenone; competitive; immunoassay; mycotoxin; quantitative detection; surface-enhanced Raman scattering