title: Determination of LBPT in human plasma by high performance liquid chromatography-tandem mass spectrometry.
abstract: A rapid and selective HPLC-MS/MS method was developed for the determination of LBPT in human plasma. The analyte was extracted from plasma samples by solid-phase extraction and then chromatographed on a C18 analytical column. The mobile phase consisted of acetonitrile-10mM ammonium formate in 0.1% formic acid (30:70, v/v) and the flow rate was 0.2 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reactions monitoring (MRM) mode using positive electrospray ionization (ESI). The method was validated over the concentration range of 0.2-100 ng/mL. Inter- and intra-day precision (RSD %) were less than 9.2% and the accuracy (RE %) ranged from 0 to 11.0%. The lower limit of quantitation (LLOQ) was 0.2 ng/mL. The extraction recovery was on average 75% and the detection was not affected by the matrix. The method was successfully applied to the pharmacokinetic study of LBPT in healthy Chinese subjects.
authors: Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, No. 1, Shuai Fu Yuan, Dong Cheng District, Beijing 100730, PR China.
authors: Ming
authors: M
authors: Liu
authors: Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, No. 1, Shuai Fu Yuan, Dong Cheng District, Beijing 100730, PR China.
authors: Hongyun
authors: H
authors: Wang
authors: Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, No. 1, Shuai Fu Yuan, Dong Cheng District, Beijing 100730, PR China.
authors: Hongzhong
authors: H
authors: Liu
authors: Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, No. 1, Shuai Fu Yuan, Dong Cheng District, Beijing 100730, PR China.
authors: Ao
authors: A
authors: Peng
authors: Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, No. 1, Shuai Fu Yuan, Dong Cheng District, Beijing 100730, PR China.
authors: Fen
authors: F
authors: Yang
authors: Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1, Xiannongtan Street, Xi Cheng District, Beijing 100050, PR China.
authors: Wenjie
authors: W
authors: Wang
authors: Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1, Xiannongtan Street, Xi Cheng District, Beijing 100050, PR China.
authors: Liya
authors: L
authors: Zhu
authors: Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1, Xiannongtan Street, Xi Cheng District, Beijing 100050, PR China.
authors: Haihong
authors: H
authors: Huang
authors: Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, No. 1, Shuai Fu Yuan, Dong Cheng District, Beijing 100730, PR China.
authors: Ji
authors: J
authors: Jiang
authors: Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, No. 1, Shuai Fu Yuan, Dong Cheng District, Beijing 100730, PR China. Electronic address: pei.hu.pumc@gmail.com.
authors: Pei
authors: P
authors: Hu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004311:Double-Blind Method; D004355:Drug Stability; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D010880:Piperidines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors*; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010880:Piperidines; D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; C000596020:ethyl 1-(5-(4-chlorophenyl)-3-oxopent-4-enyl)piperidine-4-carboxylate; C039351:platelet activating factor receptor
keywords: Chinese; HPLC–MS/MS; LBPT; Pharmacokinetics