title: A new method to assay hypoxia-inducible factor-1 based on small molecule binding DNA.
abstract: Hypoxia-inducible factor-1 (HIF-1) is among the most important indicators of hypoxia in evaluating severity of many diseases. In this work, a novel method for HIF-1 detection is proposed by using electrochemical techniques based on small molecule binding DNA. In this method, since the designed DNA sequence can specifically bind with either an electroactive small molecule or HIF-1, the signal readout is inversely proportional to HIF-1 concentration, thus a simple and easily-operated method for HIF-1 detection can be developed. With the proposed method, HIF-1 can be determined in a linear range from 5 to 25nM with a detection limit of 2.8nM. Furthermore, the proposed method can be directly used to assay HIF-1 in placenta tissue, and the assay results can reliably reflect the severity of preeclampsia, a very dangerous condition during pregnancy. The proposed method also shows desirable sensitivity, high selectivity and excellent reproducibility, so this method can have potential applications in clinical practice.
authors: Department of Obstetrics, The First Affiliated Hospital with Nanjing Medical University, Nanjing 210036, PR China.
authors: Nana
authors: N
authors: Yang
authors: Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
authors: Hao
authors: H
authors: Li
authors: Department of Obstetrics, The First Affiliated Hospital with Nanjing Medical University, Nanjing 210036, PR China.
authors: Tingting
authors: T
authors: Yin
authors: Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
authors: Yue
authors: Y
authors: Huang
authors: Department of Obstetrics, The First Affiliated Hospital with Nanjing Medical University, Nanjing 210036, PR China. Electronic address: lizhou_sun121@hotmail.com.
authors: Lizhou
authors: L
authors: Sun
authors: Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China; Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, PR China. Electronic address: genxili@nju.edu.cn.
authors: Genxi
authors: G
authors: Li
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques; D004247:DNA / Q000378:metabolism*; D017931:DNA Primers; D004566:Electrodes; D005260:Female; D006046:Gold; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy
chemical_list: D017931:DNA Primers; D051793:Hypoxia-Inducible Factor 1; D006046:Gold; D004247:DNA
keywords: Biosensor; Echinomycin; Electroanalysis; Hypoxia-inducible factor-1; Preeclampsia