title: Single-nucleotide polymorphism (c.309T>G) in theMDM2gene and lung cancer risk.
abstract: Murine double minute 2 (MDM2) is a negative regulator of p53. A single-nucleotide polymorphism (SNP) (rs2279744: c.309T>G) in the promoter region of theMDM2gene has been shown to result in higher levels of MDM2 RNA and protein. Regarding the contribution of c.309T>G in theMDM2gene to the lung cancer risk, previous studies are conflicting. In order to evaluate the association between c.309T>G and the lung cancer risk, a case-control study was performed. TheMDM2genotypes were determined in 762 lung cancer patients and in 700 cancer-free control subjects using the Smart Amplification Process. Statistical adjustment was performed for gender, age and pack-years of smoking. The distributions of c.309T>G (T/T, T/G, G/G) were 20.1, 49.7, 30.2% in the case group and 21.7, 47.9, 30.4% in the healthy-control group. There were no overall associations between theMDM2genotypes and the risk of lung cancer [T/G genotype: Adjusted odds ratio (AOR), 1.30; 95% confidence interval (CI), 0.88-1.93; and G/G genotype: AOR, 1.18; 95% CI, 0.78-1.80]. The subgroup analysis of gender, histology, smoking status and epidermal growth factor receptor mutation status also indicated that there was no association with lung cancer. Additionally, the genotypes did not have an effect on the age at the time of diagnosis of lung cancer (P=0.25). In conclusion, the G allele frequency in the lung cancer cases was 0.551, which was similar to other studies. The results of the present study suggest that the c.309T>G is not significantly associated with lung cancer.
authors: Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Yasuaki
authors: Y
authors: Enokida
authors: Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Kimihiro
authors: K
authors: Shimizu
authors: Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Seiichi
authors: S
authors: Kakegawa
authors: Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Jun
authors: J
authors: Atsumi
authors: Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Yoshiaki
authors: Y
authors: Takase
authors: Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Yohei
authors: Y
authors: Miyamae
authors: Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Toshiteru
authors: T
authors: Nagashima
authors: Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Yoichi
authors: Y
authors: Ohtaki
authors: Department of Oncology Clinical Development, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Kyoichi
authors: K
authors: Kaira
authors: Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Noriaki
authors: N
authors: Sunaga
authors: Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Noriko
authors: N
authors: Yanagitani
authors: Department of Internal Medicine, National Hospital Organization Nishi-Gunma Hospital, Shibukawa, Gunma 377-8511, Japan.
authors: Reiko
authors: R
authors: Yoshino
authors: Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Katsuhiko
authors: K
authors: Tsunekawa
authors: Department of General Thoracic Surgery, Maebashi Red Cross Hospital, Maebashi, Gunma 371-0014, Japan.
authors: Hitoshi
authors: H
authors: Igai
authors: Department of General Thoracic Surgery, Maebashi Red Cross Hospital, Maebashi, Gunma 371-0014, Japan.
authors: Mitsuhiro
authors: M
authors: Kamiyoshihara
authors: Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa 230-0045, Japan.
authors: Kengo
authors: K
authors: Usui
authors: Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa 230-0045, Japan.
authors: Alexander
authors: A
authors: Lezhava
authors: Department of Internal Medicine, National Hospital Organization Nishi-Gunma Hospital, Shibukawa, Gunma 377-8511, Japan.
authors: Yoshio
authors: Y
authors: Tomizawa
authors: NGO Personalized Medicine and Healthcare, Yokohama, Kanagawa 226-0016, Japan.
authors: Toshihisa
authors: T
authors: Ishikawa
authors: Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Masami
authors: M
authors: Murakami
authors: RIKEN Preventive Medicine and Diagnosis Innovation Program, Yokohama, Kanagawa 230-0045, Japan.
authors: Yoshihide
authors: Y
authors: Hayashizaki
authors: Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors: Izumi
authors: I
authors: Takeyoshi
keywords: cancer susceptibility; lung cancer; murine double minute 2; p53; single-nucleotide polymorphism 309; smart amplification process; smoking history