title: Generation of transgenic mice by exploiting spermatogonial stem cells in vivo.
abstract: The protocols in this chapter describe two techniques for the generation of transgenic mice by in vivo manipulation of spermatogonial stem cells (SSCs) with a high rate of success. SSCs in prepubescent animals can either be infected in vivo with recombinant lentiviruses expressing the transgene of interest or DNA can be injected into the testis followed by the application of an electric current resulting in integration of the linearized DNA containing a transgene downstream of the appropriate promoter into SSCs. All male pre-founder mice produced transgenic pups using both protocols with the transgene being heritable. Further, the pre-founder mice could be used in multiple mating experiments resulting in the generation of multiple progeny. These protocols could be extended to perform over-expression/knockdown screens in vivo using bar-coded lentiviruses/plasmid constructs, thus permitting the design of genetic screens in the mouse. Further, these protocols could be adapted to achieve transgenesis in other laboratory animals resulting in the generation of model systems that closely approximate human development and disease.
authors: Advanced Centre for Treatment Research Education and Cancer, KS-215, Tata Memorial Centre, Kharghar Node, Navi Mumbai, 410210, India.
authors: Lalit
authors: L
authors: Sehgal
authors: Abul
authors: A
authors: Usmani
authors: Sorab N
authors: SN
authors: Dalal
authors: Subeer S
authors: SS
authors: Majumdar
mesh_terms: D000818:Animals; D018274:Electroporation; D005260:Female; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D013093:Spermatogonia / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D013737:Testis / Q000378:metabolism; D013997:Time Factors; D014161:Transduction, Genetic / Q000379:methods*; D019076:Transgenes / Q000235:genetics
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins