title: Vibrio cholerae utilizes direct sRNA regulation in expression of a biofilm matrix protein.
abstract: Vibrio cholerae biofilms contain exopolysaccharide and three matrix proteins RbmA, RbmC and Bap1. While much is known about exopolysaccharide regulation, little is known about the mechanisms by which the matrix protein components of biofilms are regulated. VrrA is a conserved, 140-nt sRNA of V. cholerae, whose expression is controlled by sigma factor σE. In this study, we demonstrate that VrrA negatively regulates rbmC translation by pairing to the 5' untranslated region of the rbmC transcript and that this regulation is not stringently dependent on the RNA chaperone protein Hfq. These results point to VrrA as a molecular link between the σE-regulon and biofilm formation in V. cholerae. In addition, VrrA represents the first example of direct regulation of sRNA on biofilm matrix component, by-passing global master regulators.
authors: Department of Molecular Biology, Umeå University, Umeå, Sweden; The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden.
authors: Tianyan
authors: T
authors: Song
authors: Department of Molecular Biology, Umeå University, Umeå, Sweden; The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden.
authors: Dharmesh
authors: D
authors: Sabharwal
authors: Department of Molecular Biology, Umeå University, Umeå, Sweden; The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden.
authors: Jyoti Mohan
authors: JM
authors: Gurung
authors: Department of Microbiology and Environmental Toxicology, University of California Santa Cruz, Santa Cruz, California, United States of America.
authors: Andrew T
authors: AT
authors: Cheng
authors: Department of Molecular Biology, Umeå University, Umeå, Sweden; The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden.
authors: Annika E
authors: AE
authors: Sjöström
authors: Department of Microbiology and Environmental Toxicology, University of California Santa Cruz, Santa Cruz, California, United States of America.
authors: Fitnat H
authors: FH
authors: Yildiz
authors: Department of Molecular Biology, Umeå University, Umeå, Sweden; The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden.
authors: Bernt Eric
authors: BE
authors: Uhlin
authors: Department of Molecular Biology, Umeå University, Umeå, Sweden; The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden.
authors: Sun Nyunt
authors: SN
authors: Wai
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018441:Biofilms; D015152:Blotting, Northern; D015153:Blotting, Western; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D035001:Host Factor 1 Protein / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009154:Mutation; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism; D014734:Vibrio cholerae / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D020121:5' Untranslated Regions; D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D035001:Host Factor 1 Protein; D012329:RNA, Bacterial; D058727:RNA, Small Untranslated; D012808:Sigma Factor