title: Post- harvest consideration factors for microRNA research in cellular, tissue, serum and plasma samples.
abstract: MicroRNA plays important regulatory roles on the genetic network which provides researchers with valuable novel mechanistic directions and perspectives for applications in a wide variety of fields. Despite pertinent insight gained from messenger RNA, microRNAs possess distinct biochemical properties that emphasize the need for independent research to be conducted that addresses specifically microRNA handling. This review provides a brief summary on the various factors at post-sample harvesting stages with focus on microRNA from cellular, tissue, plasma and serum samples. It incorporates an up-to-date discussion on the isolation methods, quality control, profiling methods and other post-harvesting factors in microRNA research.
authors: Liggins Institute, The University of Auckland, Auckland, New Zealand.
authors: Vic Shao-Chih
authors: VS
authors: Chiang
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods; D053263:Gene Regulatory Networks / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D011786:Quality Control; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: carrier RNA; circulatory; isolation; microRNA; normalization; tsRNA