title: DAPT suppresses the proliferation of human glioma cell line SHG-44.
abstract: OBJECTIVE
  To explore the suppressing effect of γ-secretase inhibitor DAPT on proliferation of human glioma cell line SHG-44 in vitro and its mechanism.
  
  
  METHODS
  The SHG-44 cell was treated by DAPT with different concentration. The proliferation of cells was detected by MTT assay; cell cycle and TSC of CD133(+) were determined by flow cytometry analysis technique; the key factor in Notch signaling pathway (Notch-1, Delta-1, Hes-1) was measured by reverse transcriptase-polymerase chain reaction and western blotting.
  
  
  RESULTS
  DAPT inhibited the growth and proliferation of SHG-44 cells significantly(P<0.05). And the inhibiting effect on SHG-44 cells produced by DAPT showed a dose-dependent manner. DAPT increased the rate of cells in G0/G1 phase of SHG-44 cells, while it decreased the rate of cells in S phase. TSC of CD133(+) was significantly reduced after DAPT treated SHG-44 cells. The expression of protein and mRNA of Notch-1, Delta-1 and Hes-1 were gradually downregulated with the increase of DAPT doses.
  
  
  CONCLUSIONS
  DAPT can downregulate these key factor in Notch signaling pathway, reduce the TSC of CD133+ and inhibit the proliferation of SHG-44 cells.
authors: Department of Neurosurgery, the First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi Province 710061, China.
authors: Xin
authors: X
authors: Liu
authors: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi Province 710061, China.
authors: Qiu-Ran
authors: QR
authors: Xu
authors: Department of Neurosurgery, the First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi Province 710061, China.
authors: Wan-Fu
authors: WF
authors: Xie
authors: Department of Neurosurgery, the First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi Province 710061, China. Electronic address: maodewang@163.com.
authors: Mao-De
authors: MD
authors: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D004151:Dipeptides / Q000494:pharmacology*; D005910:Glioma; D006801:Humans; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D004151:Dipeptides; C419410:N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester
keywords: DAPT; Human glioma cell; Notch signaling pathway; SHG-44 cell line