title: [Enzymes of azurophilic neutrophil granules and matrix metalloproteinase-2 as markers of the developmental stages of experimental respiratory distress syndrome].
abstract: Acute Respiratory Distress Syndrome was reproduced in the non-linear male rats by the original method. The animals were injected lysate 45-55 thousand rat neutrophils in 0.2 mi 0.9% sodium chloride solution by puncture of the trachea (method patented RF). At each stage of syndrome development concentration of neutrophil elastase, myeloperoxidase and matrix metalloproteinase-2 in serum and bronchoalveolar lavage fluid was determined by ELISA. The increase of the concentration of neutrophil elastase and myeloperoxidase in plasma and lavage fluid has been shown to be a marker of exudative stage. Proliferative phase is marked by high levels of matrix metalloproteinase-2 at a constant content of elastase and myeloperoxidase in both liquids. Reduction of matrix metalloproteinase-2 concentration in both biological fluids is accompanied by the development of fibrotic phase distress syndrome.
authors: E V
authors: EV
authors: Prutkina
authors: A V
authors: AV
authors: Sepp
authors: N N
authors: NN
authors: Tsybikov
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D005122:Exudates and Transudates / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000097:blood* / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000097:blood / Q000378:metabolism; D009195:Peroxidase / Q000097:blood / Q000378:metabolism; D051381:Rats; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000201:enzymology* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D009195:Peroxidase; D010196:Pancreatic Elastase; D020778:Matrix Metalloproteinase 2