title: Immunomodulatory effect of a wild blueberry anthocyanin-rich extract in human Caco-2 intestinal cells.
abstract: Intestinal inflammation is a natural process crucial for the maintenance of gut functioning. However, abnormal or prolonged inflammatory responses may lead to the onset of chronic degenerative diseases, typically treated by means of pharmacological interventions. Dietary strategies for the prevention of inflammation are a safer alternative to pharmacotherapy. Anthocyanins and other polyphenols have been documented to display anti-inflammatory activity. In the present study, three bioactive fractions (anthocyanin, phenolic, and water-soluble fractions) were extracted from a wild blueberry powder. The Caco-2 intestinal model was used to test the immunomodulatory effect of the above fractions. Only the anthocyanin-rich fraction reduced the activation of NF-κB, induced by IL-1β in intestinal epithelial Caco-2 cells. Specifically, concentrations of 50 and 100 μg mL(-1) decreased NF-κB activation by 68.9 and 85.2%, respectively (p ≤ 0.05). These preliminary results provide further support for the role of food bioactives as potential dietary anti-inflammatory agents.
authors: Division of Food Microbiology and Bioprocessing and ‡Division of Human Nutrition, Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano , via Celoria 2, 20133 Milan, Italy.
authors: Valentina
authors: V
authors: Taverniti
authors: Daniela
authors: D
authors: Fracassetti
authors: Cristian
authors: C
authors: Del Bo'
authors: Claudia
authors: C
authors: Lanti
authors: Mario
authors: M
authors: Minuzzo
authors: Dorothy
authors: D
authors: Klimis-Zacas
authors: Patrizia
authors: P
authors: Riso
authors: Simone
authors: S
authors: Guglielmetti
mesh_terms: D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D029798:Blueberry Plants / Q000737:chemistry*; D018938:Caco-2 Cells; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D053583:Interleukin-1beta / Q000276:immunology; D007422:Intestines / Q000187:drug effects* / Q000276:immunology; D008954:Models, Biological; D016328:NF-kappa B / Q000276:immunology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000872:Anthocyanins; D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D016328:NF-kappa B; D010936:Plant Extracts
keywords: Caco-2 cells; NF-κB; anthocyanins; luciferase reporter system; wild blueberry