title: A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays.
abstract: We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that seen with a conventional CXCR4 antibody. This nonradioactive, direct, and CXCR4-specific high-affinity screening system provides a new platform for CXCR4-targeted drug screening, as well as for the development of new probes for other GPCRs.
authors: Department of Pharmacology, Upstate Medical University, State University of New York , Syracuse, New York 13210, United States.
authors: Yilei
authors: Y
authors: Yang
authors: Qinghao
authors: Q
authors: Zhang
authors: Mei
authors: M
authors: Gao
authors: Xiaohong
authors: X
authors: Yang
authors: Ziwei
authors: Z
authors: Huang
authors: Jing
authors: J
authors: An
mesh_terms: D000818:Animals; D001667:Binding, Competitive / Q000502:physiology; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D010446:Peptide Fragments; D019718:Receptors, CXCR4; D016650:Fluorescein-5-isothiocyanate