title: Accumulation and molecular regulation of anthocyanin in purple tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee).
abstract: Tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee) is an economically and nutritionally important dietary vegetable in Asian countries. Purple tumorous stem mustard contains inflated tumorous stem and abundant anthocyanin accumulation in leaves. Here, 20 anthocyanins were separated and identified from the purple tumorous stem mustard by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). In order to investigate the regulatory anthocyanin production in purple tumorous stem mustard, the expression of anthocyanin biosynthetic and regulatory genes in leaves from purple and green cultivars were examined. Regulatory gene BjTT8 and all biosynthetic genes were dramatically upregulated in the purple variety. Moreover, the transcript level of BjTT8 and all structural genes, except BjPAL, were all significantly higher in light-treated sprouts than in the dark. These results indicate that transcriptional activation of BjTT8 is associated with upregulation of most anthocyanin biosynthetic genes, to produce anthocyanins in purple tumorous stem mustard.
authors: Bioengineering College, Campus A, Chongqing University , 174 Shapingba Main Street, Chongqing 400030, People's Republic of China.
authors: Qiaoli
authors: Q
authors: Xie
authors: Zongli
authors: Z
authors: Hu
authors: Yanjie
authors: Y
authors: Zhang
authors: Shibing
authors: S
authors: Tian
authors: Zhijin
authors: Z
authors: Wang
authors: Zhiping
authors: Z
authors: Zhao
authors: Yang
authors: Y
authors: Yang
authors: Guoping
authors: G
authors: Chen
mesh_terms: D000872:Anthocyanins / Q000032:analysis* / Q000096:biosynthesis* / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D009149:Mustard Plant / Q000235:genetics* / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000872:Anthocyanins; D012333:RNA, Messenger; C017154:cyanidin