title: Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1.
abstract: Enterotoxins from pathogenic bacteria are known as the main reason that can cause the bacterial foodborne diseases. In this study, aptamers that bound to Staphylococcus aureus enterotoxin C1 (SEC1) with high affinity and selectivity were generated in vitro by twelve rounds of selection based on magnetic separation technology, with a low-level dissociation constant (Kd) value of 65.14 ± 11.64 nmol/L of aptamer C10. Aptamer-based quantification of SEC1 in the food sample by a graphene oxide (GO)-based method was implemented to investigate the potential of the aptamer against SEC1 with a limit of detection of 6 ng/mL. On the basis of this work, biosensors using the selected SEC1 aptamers as new molecular recognition elements could be applied for innovative determinations of SEC1.
authors: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors: Yukun
authors: Y
authors: Huang
authors: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors: Xiujuan
authors: X
authors: Chen
authors: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors: Nuo
authors: N
authors: Duan
authors: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors: Shijia
authors: S
authors: Wu
authors: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address: wangzp1974@hotmail.com.
authors: Zhouping
authors: Z
authors: Wang
authors: Collage of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.
authors: Xinlin
authors: X
authors: Wei
authors: Collage of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.
authors: Yuanfeng
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics*; D015374:Biosensing Techniques / Q000379:methods; D004768:Enterotoxins / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology; D052156:SELEX Aptamer Technique; D013211:Staphylococcus aureus / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D004768:Enterotoxins; C034959:enterotoxin C, staphylococcal
keywords: Aptamer; Food; Graphene oxide; SELEX; Staphylococcus aureus enterotoxin C1