title: Heat inactivation of the Middle East respiratory syndrome coronavirus.
abstract: The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID50 method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log10 . Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV.
authors: Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur, Paris, France; Université Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris, France.
authors: India
authors: I
authors: Leclercq
authors: Christophe
authors: C
authors: Batéjat
authors: Ana M
authors: AM
authors: Burguière
authors: Jean-Claude
authors: JC
authors: Manuguerra
mesh_terms: D018352:Coronavirus Infections / Q000821:virology*; D004203:Disinfection / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D065207:Middle East Respiratory Syndrome Coronavirus / Q000737:chemistry* / Q000502:physiology*
keywords: Heat; MERS-CoV; inactivation