title: Adsorption of Proteins to Thin-Films of PDMS and Its Effect on the Adhesion of Human Endothelial Cells.
abstract: This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.
authors: Department of Chemistry, The University of Texas at San Antonio.
authors: Karin Y
authors: KY
authors: Chumbimuni-Torres
authors: Department of Biomedical Engineering, The University of Texas at San Antonio.
authors: Ramon E
authors: RE
authors: Coronado
authors: Department of Chemistry, The University of Texas at San Antonio.
authors: Adelphe M
authors: AM
authors: Mfuh
authors: Department of Physics and Astronomy, The University of Texas at San Antonio.
authors: Carlos
authors: C
authors: Castro-Guerrero
authors: School of Agronomic Sciences - IBAM-CONICET, National University of Cuyo, Mendoza, Argentina.
authors: Maria Fernanda
authors: MF
authors: Silva
authors: Department of Chemistry, The University of Texas at San Antonio.
authors: George R
authors: GR
authors: Negrete
authors: Department of Biomedical Engineering, The University of Texas at San Antonio.
authors: Rena
authors: R
authors: Bizios
authors: Department of Chemistry, The University of Texas at San Antonio.
authors: Carlos D
authors: CD
authors: Garcia