title: Transcriptome analysis reveals the same 17 S-locus F-box genes in two haplotypes of the self-incompatibility locus of Petunia inflata.
abstract: Petunia possesses self-incompatibility, by which pistils reject self-pollen but accept non-self-pollen for fertilization. Self-/non-self-recognition between pollen and pistil is regulated by the pistil-specific S-RNase gene and by multiple pollen-specific S-locus F-box (SLF) genes. To date, 10 SLF genes have been identified by various methods, and seven have been shown to be involved in pollen specificity. For a given S-haplotype, each SLF interacts with a subset of its non-self S-RNases, and an as yet unknown number of SLFs are thought to collectively mediate ubiquitination and degradation of all non-self S-RNases to allow cross-compatible pollination. To identify a complete suite of SLF genes of P. inflata, we used a de novo RNA-seq approach to analyze the pollen transcriptomes of S2-haplotype and S3-haplotype, as well as the leaf transcriptome of the S3S3 genotype. We searched for genes that fit several criteria established from the properties of the known SLF genes and identified the same seven new SLF genes in S2-haplotype and S3-haplotype, suggesting that a total of 17 SLF genes constitute pollen specificity in each S-haplotype. This finding lays the foundation for understanding how multiple SLF genes evolved and the biochemical basis for differential interactions between SLF proteins and S-RNases.
authors: Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802.
authors: Justin S
authors: http://orcid.org/0000-0002-0195-5509
authors: JS
authors: Williams
authors: Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802.
authors: Joshua P
authors: http://orcid.org/0000-0001-9668-2525
authors: JP
authors: Der
authors: Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802 Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802.
authors: Claude W
authors: CW
authors: dePamphilis
authors: Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802 Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, Pennsylvania 16802 txk3@psu.edu.
authors: Teh-Hui
authors: TH
authors: Kao
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000235:genetics; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism; D035264:Flowers / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008040:Genetic Linkage; D056426:Genetic Loci / Q000235:genetics; D006239:Haplotypes; D032306:Petunia / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics; D011058:Pollen / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D054817:Pollination; D059748:Proteolysis; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism; D060436:Self-Incompatibility in Flowering Plants / Q000235:genetics*; D059467:Transcriptome; D054875:Ubiquitination
chemical_list: D017931:DNA Primers; D044783:F-Box Proteins; D010940:Plant Proteins; D012260:Ribonucleases