title: Chitooligosaccharides prevent osteopenia by promoting bone formation and suppressing bone resorption in ovariectomised rats: possible involvement of COX-2.
abstract: Chitooligosaccharides (CHOS) added in diet have been found as potent calcium fortifiers in conditions of Ca(2+) deficiency such as osteoporosis. In this study, we found that pharmaceutical intervention using CHOS prevented ovariectomy (OVX)-induced bone mineral density loss and the deterioration of trabecular microarchitecture in a dose-dependent manner (p < 0.05 or 0.01). CHOS (125, 250 mg/kg) suppressed the serum levels of bone resorption biomarkers CTx and TRACP5b induced by OVX (p < 0.05), but increased the levels of osteogenic markers ALP and OC by 11.3-11.6% and 10.7-15.2% of OVX group (p < 0.05), suggesting the exact pharmacological action of CHOS in the control of osteoporosis which may be the result of both promoting bone formation and suppressing bone resorption. Bone turnover-modulating effects of CHOS appear related to their anti-inflammatory capacity to down-regulate mRNA and protein expression of COX-2 (17.2-32.2% and 16.4-21.9% of OVX group, p < 0.05 or 0.01), a key mediator linking between inflammation and osteoporosis.
authors: a Department of Orthopedic Surgery , Hubei Woman and Child Hospital , Wuhan 430070 , China.
authors: Bingshu
authors: B
authors: He
authors: Jun
authors: J
authors: Wang
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001851:Bone Diseases, Metabolic / Q000188:drug therapy*; D001862:Bone Resorption / Q000188:drug therapy*; D002686:Chitin / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D005260:Female; D009844:Oligosaccharides / Q000494:pharmacology*; D010024:Osteoporosis / Q000517:prevention & control; D010052:Ovariectomy; D051381:Rats
chemical_list: D009844:Oligosaccharides; D002686:Chitin; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat
keywords: chitooligosaccharides; cyclooxygenase-2; osteoporosis