title: Tocotrienol modulates crucial lipid metabolism-related genes in differentiated 3T3-L1 preadipocytes.
abstract: Obesity and other lipid metabolism-related diseases have become more prevalent in recent years due to drastic lifestyle changes and dietary patterns. Unsaturated vitamin E, tocotrienol (T3), represents one of the most fascinating naturally occurring compounds that has the potential to influence a broad range of mechanisms underlying abnormal lipid metabolism processes. However, its efficacy and mechanism have been uncertain due to scarcity of data concerning the effect of T3 on lipid metabolism. In this study, we report a series of fascinating experimental findings on how T3 affects lipid metabolism in differentiated 3T3-L1 preadipocytes. Treatment with T3 (25 μM), especially δ and γ isomers, inhibited the accumulation of triglyceride and lipid droplets in differentiated 3T3-L1 cells. This manifestation was supported by mRNA and protein expression of crucial lipid metabolism-related genes. The present study provides a novel set of data pertaining to the possibility of T3 as an anti-metabolic disorder agent.
authors: Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan. nkgw@biochem.tohoku.ac.jp.
authors: Gregor Carpentero
authors: GC
authors: Burdeos
authors: Kiyotaka
authors: K
authors: Nakagawa
authors: Takumi
authors: T
authors: Abe
authors: Fumiko
authors: F
authors: Kimura
authors: Teruo
authors: T
authors: Miyazawa
mesh_terms: D041721:3T3-L1 Cells; D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D024508:Tocotrienols / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D024508:Tocotrienols; D014280:Triglycerides; C490333:Scd1 protein, mouse; D013230:Stearoyl-CoA Desaturase; D000123:Acetyltransferases; C055210:aminoglycoside N1-acetyltransferase