title: Potential probiotic Escherichia coli 16 harboring the Vitreoscilla hemoglobin gene improves gastrointestinal tract colonization and ameliorates carbon tetrachloride induced hepatotoxicity in rats.
abstract: The present study describes the beneficial effects of potential probiotic E. coli 16 (pUC8:16gfp) expressing Vitreoscilla hemoglobin (vgb) gene, associated with bacterial respiration under microaerobic condition, on gastrointestinal (GI) colonization and its antioxidant activity on carbon tetrachloride (CCl4) induced toxicity in Charles Foster rats. In vitro, catalase activity in E. coli 16 (pUC8:16gfp) was 1.8 times higher compared to E. coli 16 (pUC-gfp) control. In vivo, E. coli 16 (pUC8:16gfp) not only was recovered in the fecal matter after 70 days of oral administration but also retained antibacterial activities, whereas E. coli 16 (pUC-gfp) was not detected. Oral administration of 200 and 500 μL/kg body weight of CCl4 to rats at weekly interval resulted in elevated serum glutamyl pyruvate transaminase (SGPT) and serum glutamyl oxalacetate transaminase (SGOT) levels compared to controls. Rats prefed with E. coli 16 (pUC8:16gfp) demonstrated near to normal levels for SGPT and SGOT, whereas the liver homogenate catalase activity was significantly increased compared to CCl4 treated rats. Thus, pUC8:16gfp plasmid encoding vgb improved the growth and GI tract colonization of E. coli 16. In addition, it also enhanced catalase activity in rats harboring E. coli 16 (pUC8:16gfp), thereby preventing the absorption of CCl4 to GI tract.
authors: Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara Gujarat 390002, India ; C.G. Bhakta Institute of Biotechnology, Faculty of Applied Science, UKA Tarsadia University, Bardoli, Surat Gujarat 394 350, India.
authors: Prasant
authors: P
authors: Kumar
authors: Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara Gujarat 390002, India.
authors: Ayush V
authors: AV
authors: Ranawade
authors: Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara Gujarat 390002, India.
authors: Naresh G
authors: NG
authors: Kumar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002251:Carbon Tetrachloride; D002374:Catalase / Q000378:metabolism; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000235:genetics*; D005453:Fluorescence; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008107:Liver Diseases / Q000188:drug therapy* / Q000503:physiopathology; D008111:Liver Function Tests; D008297:Male; D019936:Probiotics / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D054793:Truncated Hemoglobins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D002251:Carbon Tetrachloride; D002374:Catalase