title: CELLCOUNTER: novel open-source software for counting cell migration and invasion in vitro.
abstract: Transwell Boyden chamber based migration/invasion assay is a simple and extensively used approach for the characterization of cell motility in vitro. Cell motility is quantified by counting the number of cells that pass through the filter membrane. The counting is usually performed manually, which is laborious and error prone. We have therefore developed CELLCOUNTER, an application that is capable of recognizing and counting the total number of cells through an intuitive graphical user interface. The counting can be performed in batch, and the counting results can be visualized and further curated manually. CELLCOUNTER will be helpful in streamlining the experimental process and improving the reliability of the data acquisition.
authors: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, USTC, Hefei, Anhui, China.
authors: Xiaoni
authors: 0000-0001-8758-4907
authors: X
authors: Li
authors: School of Information Science and Technology, USTC, Hefei, Anhui, China.
authors: Hongshun
authors: H
authors: Yang
authors: Analytic and Translational Genetics Unit, Massachusetts General Hospital, Boston, MA, USA ; Program in Medical and Population Genetics, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
authors: Hailiang
authors: 0000-0003-1461-5762
authors: H
authors: Huang
authors: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, USTC, Hefei, Anhui, China.
authors: Tao
authors: T
authors: Zhu
mesh_terms: D002452:Cell Count / Q000379:methods*; D045744:Cell Line, Tumor; D002465:Cell Movement; D003196:Computer Graphics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D061986:MCF-7 Cells; D009361:Neoplasm Invasiveness; D010363:Pattern Recognition, Automated / Q000379:methods*; D012984:Software