title: Structured light scatteroscopy.
abstract: A new imaging approach, structured light scatteroscopy (SLS), is demonstrated, which offers rapid wide-field imaging of microscopic morphological variations in bulk tissue surfaces. Elastic scattering of light offers exquisite sensitivity to ultrastructural changes at multiple size scales ranging from nanometers to millimeters, but in bulk tissues the confounding effects of molecular absorption and strong multiple scattering of light often lead to a dramatic reduction in scatter contrast and specificity. It is demonstrated that the SLS using structured high spatial frequency illumination and detection to probe the tissue achieves direct, absorption-independent, high-resolution maps of the scattering response. The scattering response is observed to be dependent on both the wavelength and spatial frequency of choice, indicating a potential for multiscale probing of ultrastructural changes in superficial tissue layers. This methodology can be easily applied in most wide-field imaging systems.
authors: Dartmouth College, Thayer School of Engineering, 14 Engineering Drive, Hanover, New Hampshire 03755, United States.
authors: Venkataramanan
authors: V
authors: Krishnaswamy
authors: Dartmouth College, Thayer School of Engineering, 14 Engineering Drive, Hanover, New Hampshire 03755, United States.
authors: Jonathan T
authors: JT
authors: Elliott
authors: Dartmouth College, Thayer School of Engineering, 14 Engineering Drive, Hanover, New Hampshire 03755, United States.
authors: David M
authors: DM
authors: McClatchy
authors: Dartmouth College, Geisel School of Medicine, Department of Surgery, 1 Rope Ferry Road, Hanover, New Hampshire 03755, United States.
authors: Richard J
authors: RJ
authors: Barth
authors: Dartmouth College, Geisel School of Medicine, Department of Pathology, 1 Rope Ferry Road, Hanover, New Hampshire 03755, United States.
authors: Wendy A
authors: WA
authors: Wells
authors: Dartmouth College, Thayer School of Engineering, 14 Engineering Drive, Hanover, New Hampshire 03755, United States.
authors: Brian W
authors: BW
authors: Pogue
authors: Dartmouth College, Thayer School of Engineering, 14 Engineering Drive, Hanover, New Hampshire 03755, United States.
authors: Keith D
authors: KD
authors: Paulsen
mesh_terms: D065966:Absorption, Physicochemical; D000818:Animals; D001790:Blood Physiological Phenomena; D002417:Cattle; D008027:Light; D018482:Muscle, Skeletal / Q000502:physiology; D019047:Phantoms, Imaging; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*