title: Double GC:GC mismatch in dsDNA enhances local dynamics retaining the DNA footprint: a high-resolution NMR study.
abstract: Mutations in the genome are responsible for several fatal genetic disorders. The default DNA repair mechanism restores the malfunction of the gene caused by mutation to maintain functional regularity and sequential integrity of the cell. Here, we have elucidated the NMR structure and the dynamics of GC mismatched DNA (PDB code: 2MJX) and found that the mismatched DNA still retains the typical B-type helical form, but in the process introduces backbone distortion resulting from frame-shifted base pairs.
authors: Department of Biophysics, Bose Institute, P-1/12 CIT Scheme VII (M), Kolkata 700054 (India).
authors: Anirban
authors: A
authors: Ghosh
authors: Rajiv Kumar
authors: RK
authors: Kar
authors: Janarthanan
authors: J
authors: Krishnamoorthy
authors: Subhrangsu
authors: S
authors: Chatterjee
authors: Anirban
authors: A
authors: Bhunia
mesh_terms: D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D018983:DNA Footprinting; D004260:DNA Repair / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009154:Mutation; D009690:Nucleic Acid Conformation; D013816:Thermodynamics
chemical_list: D004247:DNA
keywords: NMR spectroscopy; duplex DNA; dynamics; mismatch DNA; molecular dynamics simulation