title: ASC-1, PAT2, and P2RX5 are cell surface markers for white, beige, and brown adipocytes.
abstract: White, beige, and brown adipocytes are developmentally and functionally distinct but often occur mixed together within individual depots. To target white, beige, and brown adipocytes for diagnostic or therapeutic purposes, a better understanding of the cell surface properties of these cell types is essential. Using a combination of in silico, in vitro, and in vivo methods, we have identified three new cell surface markers of adipose cell types. The amino acid transporter ASC-1 is a white adipocyte-specific cell surface protein, with little or no expression in brown adipocytes, whereas the amino acid transporter PAT2 and the purinergic receptor P2RX5 are cell surface markers expressed in classical brown and beige adipocytes in mice. These markers also selectively mark brown/beige and white adipocytes in human tissue. Thus, ASC-1, PAT2, and P2RX5 are membrane surface proteins that may serve as tools to identify and target white and brown/beige adipocytes for therapeutic purposes.
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA. Institute for Diabetes and Obesity, Helmholtz Zentrum München, 85764 Neuherberg, Germany.
authors: Siegfried
authors: S
authors: Ussar
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA.
authors: Kevin Y
authors: KY
authors: Lee
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA. Department of Clinical Science, University of Bergen, 5020 Bergen, Norway. Hormone Laboratory, Haukeland University Hospital, 5020 Bergen, Norway.
authors: Simon N
authors: SN
authors: Dankel
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA.
authors: Jeremie
authors: J
authors: Boucher
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA.
authors: Max-Felix
authors: MF
authors: Haering
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA.
authors: Andre
authors: A
authors: Kleinridders
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA.
authors: Thomas
authors: T
authors: Thomou
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA.
authors: Ruidan
authors: R
authors: Xue
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA.
authors: Yazmin
authors: Y
authors: Macotela
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA.
authors: Aaron M
authors: AM
authors: Cypess
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA.
authors: Yu-Hua
authors: YH
authors: Tseng
authors: Department of Clinical Science, University of Bergen, 5020 Bergen, Norway. Hormone Laboratory, Haukeland University Hospital, 5020 Bergen, Norway.
authors: Gunnar
authors: G
authors: Mellgren
authors: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA. c.ronald.kahn@joslin.harvard.edu.
authors: C Ronald
authors: CR
authors: Kahn
mesh_terms: D052437:Adipocytes, Brown / Q000187:drug effects / Q000378:metabolism*; D052438:Adipocytes, White / Q000187:drug effects / Q000378:metabolism*; D058667:Adrenergic beta-3 Receptor Agonists / Q000494:pharmacology; D027182:Amino Acid Transport System y+ / Q000235:genetics / Q000378:metabolism*; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D003080:Cold Temperature; D019295:Computational Biology; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000235:genetics / Q000378:metabolism; D058485:Receptors, Purinergic P2X5 / Q000235:genetics / Q000378:metabolism*; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D058667:Adrenergic beta-3 Receptor Agonists; D027182:Amino Acid Transport System y+; D026921:Amino Acid Transport Systems, Neutral; D015415:Biomarkers; C546465:P2RX5 protein, human; C546466:P2rx5 protein, mouse; D058485:Receptors, Purinergic P2X5; C485084:SLC36A2 protein, human; C498799:SLC7A10 protein, human; C459819:Slc36a2 protein, mouse; C412624:Slc7a10 protein, mouse; D027981:Symporters