title: Bioengineering of lanthipeptides in Escherichia coli: assessing the specificity of lichenicidin and haloduracin biosynthetic machinery.
abstract: The lichenicidin and haloduracin biosynthetic machinery specificity was investigated in vivo in Escherichia coli. Unlike previous reports using different hosts, it was found that the biosynthetic machineries of lichenicidin and haloduracin are highly specific to their dedicated peptide precursors. Likewise, the substitution of lichenicidin structural genes by chimeras of lichenicidin leader sequences and haloduracin core peptides did not yield mature haloduracin peptides. Despite these restrictions, it was found that the bifunctional enzyme HalT was able to process and export lichenicidin peptides. These findings corroborate the promiscuity of LanT enzymes reported for other lantibiotics, such as nukacin ISK-1 and lacticin 481.
authors: Department of Biology and CESAM, University of Aveiro, 3810 Aveiro, Portugal. Electronic address: tcaetano@ua.pt.
authors: T
authors: T
authors: Caetano
authors: Department of Biology and CESAM, University of Aveiro, 3810 Aveiro, Portugal.
authors: J
authors: J
authors: Barbosa
authors: Institut für Chemie, Technische Universität Berlin, 10623 Berlin, Germany.
authors: E
authors: E
authors: Möesker
authors: Institut für Chemie, Technische Universität Berlin, 10623 Berlin, Germany.
authors: R D
authors: RD
authors: Süssmuth
authors: Department of Biology and CESAM, University of Aveiro, 3810 Aveiro, Portugal.
authors: S
authors: S
authors: Mendo
mesh_terms: D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics; D053898:Biosynthetic Pathways; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D001430:Bacteriocins; D011994:Recombinant Proteins; C515824:haloduracin, Bacillus halodurans; C546394:lichenicidin, Bacillus licheniformis
keywords: Bacillus; Bacteriocins; Chimeras; In vivo; Lantibiotics