title: [Telomere length of peripheral lymphocytes in patients with immuno-related pancytopenia].
abstract: OBJECTIVE
  To investigate the changes of relative telomere length (RTL) of peripheral blood (PB) CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺T lymphocytes, CD19⁺B lymphocytes and bone marrow (BM) CD34⁺ cells and its association with disease severity in untreated patients with immuno-related pancytopenia (IRP).
  
  
  METHODS
  The PB CD3⁺ , CD3⁺ CD4⁺ , CD3⁺ CD8⁺ T lymphocytes, CD19⁺ B lymphocytes, and BM CD34⁺ cells were purified by magnetic activated cell sorting (MACS), and RTL were measured with flow-fluorescence in situ hybridization (FLOW-FISH).
  
  
  RESULTS
  The RTL of CD3⁺, CD3⁺CD4⁺ , and CD3⁺CD8⁺T lymphocytes in untreated IRP patients were (27.754 ± 16.323)%, (7.526 ± 3.745)% and (25.854 ± 14.789)%, respectivly, which were significantly shorter than those in healthy-controls (54.555 ± 19.782)%, (12.096 ± 2.805)%, and (38.367 ± 4.626)% (P<0.05). The RTL of CD19⁺ lymphocytes in untreated IRP patients was (22.136 ± 16.142)%, which was significantly shorter than that in healthy controls (42.846 ± 16.353)% (P<0.01). There was no significant difference of BM CD34⁺ cells RTL between the untreated IRP patients (22.528 ± 21.601)% and the healthy controls (23.936 ± 19.822)% (P>0.05). There were significantly positive correlations between the RTL of B lymphocytes and the count of white blood cell (r=0.706, P=0.015). There were negative correlations between RTL of B lymphocytes and the clinical symptoms (r=-0.613, P=0.045) and positive correlations with therapeutic effect (r=0.775, P=0.005).
  
  
  CONCLUSIONS
  The shorter RTL of CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺, CD19⁺ lymphocytes, and the normal RTL of BM CD34⁺ cells in untreated IRP patients were identified, which might imply that IRP is a type of acquired autoimmune diseases.
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Jiangbo
authors: J
authors: Zhang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Rong
authors: R
authors: Fu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Yihao
authors: Y
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Lijuan
authors: L
authors: Li
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Hui
authors: H
authors: Liu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Kai
authors: K
authors: Ding
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Chunyan
authors: C
authors: Liu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Tian
authors: T
authors: Zhang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Shaoxue
authors: S
authors: Ding
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Erbao
authors: E
authors: Ruan
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Wen
authors: W
authors: Qu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Huaquan
authors: H
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Xiaoming
authors: X
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Guojin
authors: G
authors: Wang
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Yuhong
authors: Y
authors: Wu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Jia
authors: J
authors: Song
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Hong
authors: H
authors: Liu
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Limin
authors: L
authors: Xing
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Jing
authors: J
authors: Guan
authors: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors: Zonghong
authors: Z
authors: Shao
mesh_terms: D000293:Adolescent; D000328:Adult; D016175:B-Lymphocyte Subsets / Q000276:immunology; D002648:Child; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D008875:Middle Aged; D010198:Pancytopenia / Q000276:immunology* / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D016615:Telomere / Q000648:ultrastructure*; D055815:Young Adult