title: Regulation of the P2X7R by microRNA-216b in human breast cancer.
abstract: Breast cancer is the most common cancer in women around the world. However, the molecular mechanisms underlying breast cancer pathogenesis are only partially understood. Here, in this study, we found that P2X7R was up-regulated and miR-216b was down-regulated in breast cancer cell lines and tissues. Using bioinformatic analysis and 3'UTR luciferase reporter assay, we determined P2X7R can be directly targeted by miR-216b, which can down-regulate endogenous P2X7R mRNA and protein levels. Ectopic expression of miR-216b mimics leads to inhibited cell growth and apoptosis, while blocking expression of the miR-216b results in increased cell proliferation. Furthermore, our findings demonstrate that knockdown of P2X7R promotes apoptosis in breast cancer cells through down-regulating Bcl-2 and increasing the cleavage caspase-3 protein level. Finally, we confirmed that down-regulation of miR-216b in breast cancer is inversely associated with P2X7R expression level. Together, these findings establish miR-216b as a novel regulator of P2X7R and a potential therapeutic target for breast cancer.
authors: Department of Breast and Thyroid, Jinan Military General Hospital, Jinan 250031, Shandong Province, China.
authors: Luming
authors: L
authors: Zheng
authors: Department of General Surgery, Jinan Military General Hospital, Jinan 250031, Shandong Province, China.
authors: Xukui
authors: X
authors: Zhang
authors: Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai 200011, China.
authors: Feng
authors: F
authors: Yang
authors: Department of Breast and Thyroid, Jinan Military General Hospital, Jinan 250031, Shandong Province, China.
authors: Jian
authors: J
authors: Zhu
authors: Department of Breast and Thyroid, Jinan Military General Hospital, Jinan 250031, Shandong Province, China.
authors: Peng
authors: P
authors: Zhou
authors: Department of Breast and Thyroid, Jinan Military General Hospital, Jinan 250031, Shandong Province, China.
authors: Fang
authors: F
authors: Yu
authors: Department of Breast and Thyroid, Jinan Military General Hospital, Jinan 250031, Shandong Province, China.
authors: Lei
authors: L
authors: Hou
authors: Department of Breast and Thyroid, Jinan Military General Hospital, Jinan 250031, Shandong Province, China.
authors: Lei
authors: L
authors: Xiao
authors: Department of Breast and Thyroid, Jinan Military General Hospital, Jinan 250031, Shandong Province, China.
authors: Qingqing
authors: Q
authors: He
authors: Department of Oncology, Jinan Military General Hospital, Jinan 250031, Shandong Province, China. Electronic address: wangbaocheng4w4@163.com.
authors: Baocheng
authors: B
authors: Wang
mesh_terms: D017209:Apoptosis; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D017931:DNA Primers; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D016133:Polymerase Chain Reaction; D058486:Receptors, Purinergic P2X7 / Q000502:physiology*
chemical_list: D017931:DNA Primers; C575482:MIRN216 microRNA, human; D035683:MicroRNAs; D058486:Receptors, Purinergic P2X7
keywords: Apoptosis; Bcl-2; Breast cancer; Caspase-3; P2X7R; miR-216b