title: [Expression and significance of Beclin-1 in vasculogenic mimicry formation of gastric cancer].
abstract: OBJECTIVE
  To explore the effect and mechanism of autophagy specific gene Beclin-1 in gastric cancer cell SGC7901 on vasculogenic mimicry (VM) forming ability.
  
  
  METHODS
  Plasmid vectors with and without integrated shRNA were transfected respectively into SGC7901 cell line (Beclin1-inhibited group and negative control group). Simple SGC7901 cell line was used as blank group. RT-PCR and Western blot were performed to examine the expression of Beclin-1 in 3 groups. Culture was used to construct the VM model in vitro. Different VM forming ability was measured and genes (beclin-1, notch-1) expression of each group was detected before and after VM formation.
  
  
  RESULTS
  Beclin-1 and notch-1 expression increased significantly in the process of VM forming. When beclin-1 was inhibited, the formation of VM was limited and VM formative genes expression decreased. As compared to cells of negative control group, those of Beclin1-inhibited group had less number of VM forming cellular tube-like construction (15.4±1.1 vs. 37.8±1.9, P<0.05), shorter length of such construction [(316.8±24.6) mm vs. (385.1±14.2) mm, P<0.05], and less crossing point (11.6±1.1 vs. 27.2±1.1, P<0.05).
  
  
  CONCLUSIONS
  Beclin-1 can promote VM formation through maintaining stable expression of gastric cancer cell VM regulating genes. Beclin-1 inhibition may be a new target for gastric cancer gene therapy.
authors: Department of Gastrointestinal Surgery, The Second Affiliated Hospital, Suzhou University, Suzhou 225000, China. xingcg@126.com.
authors: Yunpeng
authors: Y
authors: Ding
authors: Kui
authors: K
authors: Zhao
authors: Yong
authors: Y
authors: Wu
authors: Chungen
authors: C
authors: Xing
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001343:Autophagy; D000071186:Beclin-1; D045744:Cell Line, Tumor; D005822:Genetic Vectors; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D034741:RNA, Small Interfering; D013274:Stomach Neoplasms / Q000098:blood supply / Q000378:metabolism*; D014162:Transfection
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D008565:Membrane Proteins; D034741:RNA, Small Interfering