title: miRNAs and genes expression in MARC-145 cell in response to PRRSV infection.
abstract: The regulation of viral replication is under control of miRNAs and their target genes. Several articles report the cross-talk between host and virus. The drastic effects of Porcine reproductive and respiratory syndrome virus (PRRSV) pressed us to investigate the expression profiling of miRNAs and immunity related genes during PRRSV infection. This was performed by qPCR in MARC145 cells during PRRSV infection. It was observed that miRNAs and genes show different expression patterns at different time points during PRRSV infection. The early infected stage was accompanied with increased expression of some miRNAs including miR-204, miR-21, miR-181a, miR-29 while a decrease was observed for the same in late infection stage. The opposite condition also existed in parallel. An interesting observation was seen when miR-145 was strongly induced by PRRSV infection, whereas miR-127 expression was significantly reduced in all infection points. Taken together, our studies have revealed that the expressions of miRNAs and immune-related genes were regulated in PRRSV infected MARC-145 cells and had important roles in the immune response, providing a basis for further investigations.
authors: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
authors: Ao
authors: A
authors: Zhou
authors: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
authors: Shuaifeng
authors: S
authors: Li
authors: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: sjxiaozhang@mail.hzau.edu.cn.
authors: Shujun
authors: S
authors: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D019295:Computational Biology; D003588:Cytopathogenic Effect, Viral; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D035683:MicroRNAs / Q000235:genetics*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000235:genetics / Q000276:immunology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D013552:Swine
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Expression; Immunity-related genes; PRRSV; miRNA