title: Comparison of quantitative and qualitative tests for glucose-6-phosphate dehydrogenase deficiency.
abstract: A barrier to eliminating Plasmodium vivax malaria is inadequate treatment of infected patients. 8-Aminoquinoline-based drugs clear the parasite; however, people with glucose-6-phosphate dehydrogenase (G6PD) deficiency are at risk for hemolysis from these drugs. Understanding the performance of G6PD deficiency tests is critical for patient safety. Two quantitative assays and two qualitative tests were evaluated. The comparison of quantitative assays gave a Pearson correlation coefficient of 0.7585 with significant difference in mean G6PD activity, highlighting the need to adhere to a single reference assay. Both qualitative tests had high sensitivity and negative predictive value at a cutoff G6PD value of 40% of normal activity if interpreted conservatively and performed under laboratory conditions. The performance of both tests dropped at a cutoff level of 45%. Cytochemical staining of specimens confirmed that heterozygous females with > 50% G6PD-deficient cells can seem normal by phenotypic tests.
authors: Nicole
authors: N
authors: LaRue
authors: Maria
authors: M
authors: Kahn
authors: Marjorie
authors: M
authors: Murray
authors: Brandon T
authors: BT
authors: Leader
authors: Pooja
authors: P
authors: Bansil
authors: Sarah
authors: S
authors: McGray
authors: Michael
authors: M
authors: Kalnoky
authors: Hao
authors: H
authors: Zhang
authors: Huiqiang
authors: H
authors: Huang
authors: Hui
authors: H
authors: Jiang
authors: Gonzalo J
authors: GJ
authors: Domingo
mesh_terms: D000328:Adult; D000634:Aminoquinolines / Q000009:adverse effects*; D000962:Antimalarials / Q000009:adverse effects*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D006579:Heterozygote; D006801:Humans; D016780:Malaria, Vivax / Q000188:drug therapy*; D008297:Male; D008875:Middle Aged; D010966:Plasmodium vivax / Q000187:drug effects*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000634:Aminoquinolines; D000962:Antimalarials; D005954:Glucosephosphate Dehydrogenase; C080436:8-aminoquinoline