title: Meta-analysis of quantitative trait loci for grain yield and component traits under reproductive-stage drought stress in an upland rice population.
abstract: A recombinant inbred population developed from a cross between high-yielding lowland rice (Oryza sativaL.) subspeciesindicacv. IR64 and upland tropical rice subspeciesjaponicacv. Cabacu was used to identify quantitative trait loci (QTLs) for grain yield (GY) and component traits under reproductive-stage drought stress. One hundred fifty-four lines were grown in field trials in Indonesia under aerobic conditions by giving surface irrigation to field capacity every 4 days. Water stress was imposed for a period of 15 days during pre-flowering by withholding irrigation at 65 days after seeding. Leaf rolling was scored at the end of the stress period and eight agronomic traits were evaluated after recovery. The population was also evaluated for root pulling force, and a total of 201 single nucleotide polymorphism markers were used to construct the molecular genetic linkage map and QTL mapping. A QTL for GY under drought stress was identified in a region close to thesd1locus on chromosome 1. QTL meta-analysis across diverse populations showed that this QTL was conserved across genetic backgrounds and co-localized with QTLs for leaf rolling and osmotic adjustment (OA). A QTL for percent seed set and grains per panicle under drought stress was identified on chromosome 8 in the same region as a QTL for OA previously identified in three different populations.
authors: Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor, 16111 Indonesia.
authors: Kurniawan R
authors: KR
authors: Trijatmiko
authors: Banguntapan Field Station, Faculty of Agriculture, Gadjah Mada University, Jl. Socio Justicia Bulaksumur, Yogyakarta, 55281 Indonesia.
authors: Supriyanta
authors: Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor, 16111 Indonesia.
authors: Joko
authors: J
authors: Prasetiyono
authors: International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines.
authors: Michael J
authors: MJ
authors: Thomson
authors: International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines.
authors: Casiana M
authors: CM
authors: Vera Cruz
authors: Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor, 16111 Indonesia.
authors: Sugiono
authors: S
authors: Moeljopawiro
authors: Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR USA ; Virginia Bioinformatics Institute, Virginia Polytechnic and State University, Blacksburg, VA USA.
authors: Andy
authors: A
authors: Pereira
keywords: Drought tolerance; Grain yield; Quantitative trait loci; Recombinant inbred lines; Reproductive-stage drought; Spikelet fertility