title: Temporal control over the initiation of cell motility by a regulator of G-protein signaling.
abstract: The control over the acquisition of cell motility is central for a variety of biological processes in development, homeostasis, and disease. An attractive in vivo model for investigating the regulation of migration initiation is that of primordial germ cells (PGCs) in zebrafish embryos. In this study, we show that, following PGC specification, the cells can polarize but do not migrate before the time chemokine-encoded directional cues are established. We found that the regulator of G-protein signaling 14a protein, whose RNA is a newly identified germ plasm component, regulates the temporal relations between the appearance of the guidance molecules and the acquisition of cellular motility by regulating E-cadherin levels.
authors: Institute for Cell Biology, Center for Molecular Biology of Inflammation, Münster University, D-48149 Münster, Germany;
authors: Johannes
authors: J
authors: Hartwig
authors: Institute for Cell Biology, Center for Molecular Biology of Inflammation, Münster University, D-48149 Münster, Germany;
authors: Katsiaryna
authors: K
authors: Tarbashevich
authors: Integrated Functional Genomics, Interdisciplinary Center for Clinical Research, Münster University, D-48149 Münster, Germany; and.
authors: Jochen
authors: J
authors: Seggewiß
authors: Flow Cytometry Unit, Max Planck Institute for Molecular Biomedicine, D-48149 Münster, Germany.
authors: Martin
authors: M
authors: Stehling
authors: Institute for Cell Biology, Center for Molecular Biology of Inflammation, Münster University, D-48149 Münster, Germany;
authors: Jan
authors: J
authors: Bandemer
authors: Institute for Cell Biology, Center for Molecular Biology of Inflammation, Münster University, D-48149 Münster, Germany;
authors: Cecilia
authors: C
authors: Grimaldi
authors: Institute for Cell Biology, Center for Molecular Biology of Inflammation, Münster University, D-48149 Münster, Germany;
authors: Azadeh
authors: A
authors: Paksa
authors: Institute for Cell Biology, Center for Molecular Biology of Inflammation, Münster University, D-48149 Münster, Germany;
authors: Theresa
authors: T
authors: Groß-Thebing
authors: Institute for Cell Biology, Center for Molecular Biology of Inflammation, Münster University, D-48149 Münster, Germany;
authors: Dana
authors: D
authors: Meyen
authors: Institute for Cell Biology, Center for Molecular Biology of Inflammation, Münster University, D-48149 Münster, Germany; erez.raz@uni-muenster.de.
authors: Erez
authors: E
authors: Raz
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D016764:Cell Polarity / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D020710:RGS Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015820:Cadherins; D020710:RGS Proteins; D012333:RNA, Messenger; D029961:Zebrafish Proteins
keywords: cell adhesion; rgs protein