title: Wound healing potential of antibacterial microneedles loaded with green tea extracts.
abstract: This study evaluates the utility of an antibacterial microneedle composed of green tea (GT) extract and hyaluronic acid (HA), for the efficient delivery of GT. These microneedles have the potential to be a patient-friendly method for the conventional sustained release of drugs. In this study, a fabrication method using a mold-based technique to produce GT/HA microneedles with a maximum area of ~50mm(2) with antibacterial properties was used to manufacture transdermal drug delivery systems. Fourier transform infrared (FTIR) spectrometry was carried out to observe the potential modifications in the microneedles, when incorporated with GT. The degradation rate of GT in GT/HA microneedles was controlled simply by adjusting the HA composition. The effects of different ratios of GT in the HA microneedles were determined by measuring the release properties. In HA microneedles loaded with 70% GT (GT70), a continuous higher release rate was sustained for 72h. The in vitro cytotoxicity assays demonstrated that GT/HA microneedles were not generally cytotoxic to Chinese hamster ovary cells (CHO-K1), human embryonic kidney cells (293T), and mouse muscle cells (C2C12), which were treated for 12 and 24h. Antimicrobial activity of the GT/HA microneedles was demonstrated by ~95% growth reduction of gram negative [Escherichia coli (E. coli), Pseudomonas putida (P. putida), and Salmonella typhimurium (S. typhimurium)] and gram positive bacteria [Staphylococcus aureus (S. Aureus) and Bacillus subtilis (B. subtilis)], with GT70. Furthermore, GT/HA microneedles reduced bacterial growth of infected wound sites in the skin and improved wound healing process of skin in rat model.
authors: Division of Materials Science, Korea Basic Science Institute (KBSI), Daejeon 305-333, Republic of Korea.
authors: So Young
authors: SY
authors: Park
authors: Division of Materials Science, Korea Basic Science Institute (KBSI), Daejeon 305-333, Republic of Korea. Electronic address: leeho@kbsi.re.kr.
authors: Hyun Uk
authors: HU
authors: Lee
authors: Department of BioNano Technology, Gachon University, 1342 Seongnamdaero, Sujeong-gu, Seongnam-si, Gyeonggi-do 461-701, Republic of Korea.
authors: Young-Chul
authors: YC
authors: Lee
authors: Division of Life Science, Korea Basic Science Institute (KBSI), Daejeon 305-333, Republic of Korea.
authors: Gun Hwa
authors: GH
authors: Kim
authors: Division of Life Science, Korea Basic Science Institute (KBSI), Daejeon 305-333, Republic of Korea.
authors: Edmond Changkyun
authors: EC
authors: Park
authors: Small Lab Co., Ltd., Daejeon 305-509, Republic of Korea.
authors: Seung Hyun
authors: SH
authors: Han
authors: Small Lab Co., Ltd., Daejeon 305-509, Republic of Korea.
authors: Jeong Gyu
authors: JG
authors: Lee
authors: College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors: Saehae
authors: S
authors: Choi
authors: R&D Center, Sugentech, Inc., Daejeon Bioventure Town 461-8, Daejeon 305-811, Republic of Korea.
authors: Nam Su
authors: NS
authors: Heo
authors: Division of Materials Science, Korea Basic Science Institute (KBSI), Daejeon 305-333, Republic of Korea.
authors: Dong Lak
authors: DL
authors: Kim
authors: Department of Biological Engineering, College of Engineering, Inha University, Incheon 402-751, Republic of Korea.
authors: Yun Suk
authors: YS
authors: Huh
authors: Division of Materials Science, Korea Basic Science Institute (KBSI), Daejeon 305-333, Republic of Korea. Electronic address: jouhahn@kbsi.re.kr.
authors: Jouhahn
authors: J
authors: Lee
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections; D016466:CHO Cells; D028241:Camellia sinensis / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D057809:HEK293 Cells; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D008297:Male; D055616:Microtechnology / Q000295:instrumentation*; D009339:Needles; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D057968:Transdermal Patch; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D006820:Hyaluronic Acid
keywords: Antibacterial activity; Green tea extract; Hyaluronic acid; Microneedles; Transdermal drug delivery; Wound healing