title: Tissue factor expression and methylation regulation in differentiation of embryonic stem cells into trophoblast.
abstract: OBJECTIVE
  To explore tissue factor (TF) expression and methylation regulation in differentiation of human embryonic stem cells (hESCs) into trophoblast.
  
  
  METHODS
  Differentiation of hESCs into trophoblast was induced by bone morphogenetic protein 4 (BMP4). Expression of gene, protein of TF and DNA methylation at different time points during induction process was detected by RT-PCT, Western blot, flow cytometry and MSP-PCR method.
  
  
  RESULTS
  The expression of mRNA, protein level of TF could be detected during directional differentiation of hESCs to trophoblast cells, semi methylation-semi non methylation expression appeared at TF DNA promoter region, and it showed decreased methylation level and increased non methylation level with formation of trophoblast cell and increased expression of TF.
  
  
  CONCLUSIONS
  It shows that during differentiation of hESCs into trophoblast, the differential expression of TF is related with DNA methylation level, and it is changed with the methylation or non methylated degree. It provids new platform to furtherly explore the regulation mechanisms of specific expression of tissue factor in the process of the embryonic stem cell development.
authors: Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors: Lin-Xin
authors: LX
authors: Liu
authors: Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors: Hui
authors: H
authors: Zeng
authors: Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors: En-Yi
authors: EY
authors: Liu
authors: Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors: Fang-Ping
authors: FP
authors: Chen
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002460:Cell Line; D019175:DNA Methylation / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000502:physiology*; D006801:Humans; D051381:Rats; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism* / Q000502:physiology
chemical_list: D055415:Bone Morphogenetic Protein 4; D013925:Thromboplastin
keywords: Embryonic stem cells; Methylation; Tissue factor; Trophoblast cell