title: Development and characterization of polyphenon 60 and caffeine microemulsion for enhanced antibacterial activity.
abstract: Green tea catechins and caffeine have exhibited antibacterial activity; however, their use is limited by lack of stability and effective delivery systems. Polyphenon 60 (P60) and caffeine were encapsulated in a single microemulsion (ME) formulation with an objective to lower the minimum inhibitory concentrations (MICs) of the individual agents against selected pathogens (S. epidermidis and E. coli). Combination of two natural compounds would advocate two different mechanisms on the bacterial growth thereby providing for better antibacterial activity. Thermodynamically stable ME was developed and characterized with an average particle size of 17.58 nm, further confirmed by TEM analysis. Antibacterial studies included chequerboard microdilution assay to determine the MIC and fractional inhibitory concentration (FIC) of both the natural compounds individually and in combination. MIC and FIC results indicated that the combination of the above two natural compounds was proficient in lowering the MICs of individual agents. Results of DPPH assay indicated that ME system preserved the long term antioxidative potential of P60 and caffeine. The cytotoxicity of the optimized formulation on Vero cell line by MTT assay was found to be nontoxic to mammalian cells.
authors: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, Uttar Pradesh 201307, India.
authors: Sonal
authors: S
authors: Gupta
authors: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, Uttar Pradesh 201307, India.
authors: Rakhi
authors: R
authors: Bansal
authors: Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi 110062, India.
authors: Javed
authors: J
authors: Ali
authors: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, Uttar Pradesh 201307, India.
authors: Reema
authors: R
authors: Gabrani
authors: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, Uttar Pradesh 201307, India.
authors: Shweta
authors: S
authors: Dang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002522:Chlorocebus aethiops; D002839:Chromans / Q000378:metabolism; D004655:Emulsions; D004926:Escherichia coli / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D013212:Staphylococcus epidermidis / Q000187:drug effects; D055672:Static Electricity; D014709:Vero Cells
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D002839:Chromans; D004655:Emulsions; D005419:Flavonoids; D016166:Free Radical Scavengers; D002110:Caffeine; D002392:Catechin; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid