title: Extracellular matrix of galectin-1-exposed dermal and tumor-associated fibroblasts favors growth of human umbilical vein endothelial cells in vitro: a short report.
abstract: OBJECTIVE
  Stromal cells in the tumor microenvironment are primarily considered as sources of promalignant factors. The objective of our study was to define the effect of extracellular matrix (ECM) produced by normal dermal or cancer-associated fibroblasts exposed to adhesion/growth-regulatory lectin galectin-1 on human umbilical vein endothelial cells (HUVECs).
  
  
  METHODS
  Fibroblasts were cultured for 10 days with lectin, followed by removing cellular constituents after an osmotic shock. Freshly-isolated HUVECs were placed on the ECM. In parallel, HUVECs were seeded on untreated and gelatin-coated surfaces as controls. A positive control for growth of HUVECs culture using medium supplemented with vascular endothelial growth factor completed the test panel. Cells were kept in contact to the substratum for two days and then processed for immunocytochemistry.
  
  
  RESULTS
  HUVECs seeded on fibroblast-generated ECM presented a comparatively high degree of proliferation. Furthermore, contact to substratum produced by tumor-associated fibroblasts led to generation of a meshwork especially rich in fibronectin.
  
  
  CONCLUSIONS
  Galectin-1 is apparently capable to trigger ECM production favorable for growth of HUVECs, prompting further work on characterizing structural features of the ECM and in situ correlation of lectin presence, ECM constitution and neoangiogenesis.
authors: Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic Department of Pathological Anatomy, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic.
authors: Vlasta
authors: V
authors: Peržeľová
authors: Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic.
authors: Lenka
authors: L
authors: Varinská
authors: Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
authors: Barbora
authors: B
authors: Dvořánková
authors: Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
authors: Pavol
authors: P
authors: Szabo
authors: Department of Cardiology, East-Slovak Institute of Cardiovascular Diseases Inc., Košice, Slovak Republic.
authors: Peter
authors: P
authors: Spurný
authors: Department of Stomatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
authors: Jaroslav
authors: J
authors: Valach
authors: Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic.
authors: Ján
authors: J
authors: Mojžiš
authors: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilian University, Munich, Germany.
authors: Sabine
authors: S
authors: André
authors: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilian University, Munich, Germany.
authors: Hans-Joachim
authors: HJ
authors: Gabius
authors: Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic galovci@yahoo.com pgal@vusch.sk karel.smetana@lf1.cuni.cz.
authors: Karel
authors: K
authors: Smetana
authors: Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic Department of Biomedical Research, East-Slovak Institute of Cardiovascular Diseases Inc., Košice, Slovak Republic galovci@yahoo.com pgal@vusch.sk karel.smetana@lf1.cuni.cz.
authors: Peter
authors: P
authors: Gál
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000502:physiology*; D037483:Galectin 1 / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology; D006801:Humans; D059016:Tumor Microenvironment; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: D037483:Galectin 1; D042461:Vascular Endothelial Growth Factor A
keywords: Angiogenesis; extracellular matrix; lectin; tumor growth; wound healing