title: Culture of the green microalga Botryococcus braunii Showa with LED irradiation eliminating violet light enhances hydrocarbon production and recovery.
abstract: The green microalga Botryococcus braunii (B. braunii), race B, was cultured under light-emitting diode (LED) irradiation with and without violet light. This study examined the effect of violet light on hydrocarbon recovery and production in B. braunii. C34 botryococcene hydrocarbons were efficiently extracted by thermal pretreatments at lower temperatures when the alga was cultured without violet light. The hydrocarbon content was also higher (approximately 3%) in samples cultured without violet light. To elucidate the mechanism of effective hydrocarbon recovery and production, we examined structural components of the extracellular matrix (ECM). The amounts of extracellular carotenoids and water-soluble polymers extracted by thermal pretreatment from the ECM were decreased when the alga was cultured without violet light. These results indicate that LED irradiation without violet light is more effective for hydrocarbon recovery and production in B. braunii. Furthermore, structural ECM components are closely involved in hydrocarbon recovery and production in B. braunii.
authors: a Graduate School of Agricultural and Life Sciences, The University of Tokyo , Tokyo , Japan.
authors: Sueko
authors: S
authors: Atobe
authors: Kiyotaka
authors: K
authors: Saga
authors: Haruko
authors: H
authors: Maeyama
authors: Kazuhiro
authors: K
authors: Fujiwara
authors: Shigeru
authors: S
authors: Okada
authors: Kenji
authors: K
authors: Imou
mesh_terms: D001704:Biopolymers / Q000096:biosynthesis / Q000737:chemistry; D002338:Carotenoids / Q000378:metabolism; D000460:Chlorophyta / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D003116:Color; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D005109:Extracellular Matrix / Q000378:metabolism / Q000528:radiation effects; D006838:Hydrocarbons / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008027:Light; D012995:Solubility; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D001704:Biopolymers; D006838:Hydrocarbons; D014867:Water; D002338:Carotenoids
keywords: Botryococcus braunii; biofuel; extracellular matrix; hydrocarbon; violet light