title: The nutritional composition and anti-hypertensive activity on spontaneously hypertensive rats of sipuncula Phascolosoma esculenta.
abstract: Recent studies have investigated anti-hypertensive peptides derived from natural food products. In this work, we focus on Phascolosoma esculenta as a resource of anti-hypertensive peptides, which is also a seafood with high nutritive value. Compared with FAO/WHO requirements, P. esculenta was confirmed to contain high contents of amino acids and minerals. To investigate the anti-hypertensive activity of P. esculenta, water-soluble and insoluble proteins were extracted and hydrolysed by pepsin and trypsin, respectively. The hydrolysates of water-soluble proteins derived by pepsin and pepsin-trypsin exhibited angiotensin-converting enzyme (ACE) inhibitory activity with IC50 values of 0.67 and 0.24 mg ml(-1), respectively, and those of water-insoluble proteins presented IC50 values of 0.4 and 0.1 mg ml(-1), respectively. Experiments on 'spontaneously hypertensive rats' (SHRs) were carried out to test the anti-hypertension activity in vivo, which confirmed that the hydrolysates played a significant role in reducing both diastolic blood pressure (DBP) and systolic blood pressure (SBP). Consistently, the in vivo anti-hypertensive activity of the hydrolysis products of pepsin and trypsin used together was also higher than that by using pepsin hydrolysis products alone. As stated in both sets of results, we believe that P. esculenta is an excellent resource of antihypertensive peptides and warrants further investigation.
authors: State Key Laboratory of Bioreactor Engineering, Department of Food Science and Engineering, East China University of Science and Technology, 130 # Meilong Rd, Shanghai 200237, P. R. China. jlxie@ecust.edu.cn.
authors: Yanling
authors: Y
authors: Wu
authors: Ming
authors: M
authors: Fang
authors: Lei
authors: L
authors: Du
authors: Hongxi
authors: H
authors: Wu
authors: Yalan
authors: Y
authors: Liu
authors: Mingrong
authors: M
authors: Guo
authors: Jingli
authors: J
authors: Xie
authors: Dongzhi
authors: D
authors: Wei
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage* / Q000737:chemistry; D001794:Blood Pressure / Q000187:drug effects; D006801:Humans; D006868:Hydrolysis; D006973:Hypertension / Q000188:drug therapy* / Q000201:enzymology / Q000503:physiopathology; D008297:Male; D009753:Nutritive Value; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011077:Polychaeta / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000008:administration & dosage*; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D011492:Protein Hydrolysates; D007703:Peptidyl-Dipeptidase A