title: Morin hydrate augments phagocytosis mechanism and inhibits LPS induced autophagic signaling in murine macrophage.
abstract: Morin, a natural flavonoid that is the primary bioactive constituent of the family Moraceae, has been found to be associated with many therapeutic properties. In this study, we evaluated the immunomodulatory activities of increasing concentration of morin hydrate in vitro. Three different concentrations of morin hydrate (5, 10, and 15μM) were used to evaluate their effect on splenocyte proliferation, phagocytic activity of macrophages, cytokine secretion and complement inhibition. We also evaluated the role of morin hydrate on lipopolysaccharide (LPS) induced autophagy. Our study demonstrated that morin hydrate elicited a significant increase in splenocyte proliferation, phagocytic capacity and suppressed the production of cytokines and nitric oxide in activated macrophages. Humoral immunity measured by anti-complement activity showed an increase in inhibition of the complement system after the addition of morin hydrate, where morin hydrate at 15μM concentration induced a significant inhibition. Depending on our results, we can also conclude that morin hydrate protects macrophages from LPS induced autophagic cell death. Our findings suggest that morin hydrate represents a structurally diverse class of flavonoid and this structural variability can profoundly affect its cell-type specificity and its biological activities. Supplementation of immune cells with morin hydrate has an upregulating and immunoprotective effect that shows potential as a countermeasure to the immune dysfunction and suggests an interesting use in inflammation related diseases.
authors: Department of Biotechnology, Daegu University, Kyoungsan, Kyoungbook 712-714, Republic of Korea.
authors: Rekha
authors: R
authors: Jakhar
authors: Department of Biotechnology, Daegu University, Kyoungsan, Kyoungbook 712-714, Republic of Korea.
authors: Souren
authors: S
authors: Paul
authors: Department of Biotechnology, Daegu University, Kyoungsan, Kyoungbook 712-714, Republic of Korea.
authors: Anil Kumar
authors: AK
authors: Chauhan
authors: Department of Biotechnology, Daegu University, Kyoungsan, Kyoungbook 712-714, Republic of Korea. Electronic address: sckang@daegu.ac.kr.
authors: Sun Chul
authors: SC
authors: Kang
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003167:Complement Activation / Q000187:drug effects; D015232:Dinoprostone / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*; D016897:Respiratory Burst / Q000187:drug effects; D013154:Spleen / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D047309:Flavones
chemical_list: D015232:Dinoprostone; D005419:Flavonoids; D007155:Immunologic Factors; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D014409:Tumor Necrosis Factor-alpha; C008548:morin; D047309:Flavones
keywords: Anticomplement; Autophagy; Cytokine; Immunomodulation; Nitric oxide; Phagocytosis