title: Detection of anti-p155/140, anti-p140, and antiendothelial cells autoantibodies in patients with juvenile dermatomyositis.
abstract: OBJECTIVE
  The pathogenesis of juvenile dermatomyositis (JDM), the most common idiopathic inflammatory myopathy in children, is unclear. The identification of novel autoantibodies in JDM may have clinical implications. The aim of this study was to assess the presence of anti-p155/140, anti-p140 antibodies, and antiendothelial cells antibodies (AECA) in patients with JDM and to correlate autoantibodies with clinical manifestations.
  
  
  METHODS
  Serum AECA against human umbilical vein endothelial cells were detected by enzyme-linked immunosorbent assay in 25 patients with JDM and 17 normal controls. Immunoblotting was performed to detect serum anti-p155/140 and anti-p140 antibodies.
  
  
  RESULTS
  Patients with JDM had significantly higher serum levels of AECA than healthy controls (p = 0.002). Nineteen patients (76%) and five control patients (29.4%) had positive AECAs (p = 0.003). The cutoff point of serum levels of AECA was determined by the receiver operating characteristic (ROC) curve analysis. Anti-p155/140 and anti-p140 antibodies were detected in 9 patients and 7 patients with JDM (36% and 28%, respectively). Anti-p155/140 antibodies were significantly associated with higher proportion of ESR elevation (100% vs. 0%, p = 0.006), higher erythrocyte sedimentation rate levels at diagnosis (40.3 ± 15.5 vs. 13.4 ± 5.3, p = 0.019), and a younger age at diagnosis (5.2 ± 3.2 years vs. 8.0 ± 3.0 years, p = 0.03).
  
  
  CONCLUSIONS
  anti-p155/140, anti-p140, and AECA antibodies are significantly associated with JDM. The roles of autoantibodies in the pathogenesis await further investigation.
authors: Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
authors: Hsin-Hui
authors: HH
authors: Yu
authors: Department of Pediatrics, Buddhist Tzu-Chi General Hospital, Hualien, Taiwan.
authors: Hsien-Mei
authors: HM
authors: Chang
authors: Graduate Institute of Immunology, National Taiwan University, Taipei, Taiwan.
authors: Chiao-Juno
authors: CJ
authors: Chiu
authors: Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
authors: Yao-Hsu
authors: YH
authors: Yang
authors: Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
authors: Jyh-Hong
authors: JH
authors: Lee
authors: Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
authors: Li-Chieh
authors: LC
authors: Wang
authors: Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
authors: Yu-Tsan
authors: YT
authors: Lin
authors: Department of Medical Research, National Taiwan University Hospital, Taipei, Taiwan. Electronic address: gicmbor@ntu.edu.tw.
authors: Bor-Luen
authors: BL
authors: Chiang
mesh_terms: D000293:Adolescent; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003882:Dermatomyositis / Q000473:pathology*; D042783:Endothelial Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007223:Infant; D008297:Male; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D001324:Autoantigens
keywords: Juvenile dermatomyositis (JDM); anti-endothelial cell antibodies; anti-p140; anti-p155/140; autoantibodies