title: WNT16B from ovarian fibroblasts induces differentiation of regulatory T cells through β-catenin signal in dendritic cells.
abstract: Treatment for cancer can induce a series of secreted factors into the tumor microenvironment, which can affect cancer progression. Wingless-type MMTV (mouse mammary tumor virus) integration site 16B (WNT16B) is a new member of the WNT family and has been reported to play growth-related roles in previous studies. In this study, we found WNT16B could be expressed and secreted into the microenvironment by human ovarian fibroblasts after DNA damage-associated treatment, including chemotherapy drugs and radiation. We also demonstrated that fibroblast-derived WNT16B could result in accumulation of β-catenin in dendritic cells and secretion of interleukin-10 (IL-10) and transforming growth factor beta (TGF-β), which contributed to the differentiation of regulatory T cells in a co-culture environment. These results shed light on the roles of WNT16B in immune regulation, especially in regard to cancer treatment.
authors: State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. shencongcongbio@163.com.
authors: Cong-Cong
authors: CC
authors: Shen
authors: State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. kangyuhuan@yeah.net.
authors: Yu-Huan
authors: YH
authors: Kang
authors: Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China. zhaomingbio@yeah.net.
authors: Ming
authors: M
authors: Zhao
authors: State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. heyidoctor@126.com.
authors: Yi
authors: Y
authors: He
authors: Department of Medical Oncology, the Fifth People's Hospital of Chengdu, Chengdu 611130, China. cuixinyimd@163.com.
authors: Dan-Dan
authors: DD
authors: Cui
authors: State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. fuyuyinbio@yeah.net.
authors: Yu-Yin
authors: YY
authors: Fu
authors: Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China. zhaomingbio@yeah.net.
authors: Ling-Lin
authors: LL
authors: Yang
authors: State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. goulantu@gmail.com.
authors: Lan-Tu
authors: LT
authors: Gou
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism*; D010053:Ovary / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D059016:Tumor Microenvironment; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; C508609:IL10 protein, human; D016212:Transforming Growth Factor beta; C417262:WNT16 protein, human; D051153:Wnt Proteins; D051176:beta Catenin; D016753:Interleukin-10