title: Circulating miR-148b and miR-133a as biomarkers for breast cancer detection.
abstract: Circulating microRNAs have drawn a great deal of attention as promising novel biomarkers for breast cancer. However, to date, the results are mixed. Here, we performed a three-stage microRNA analysis using plasma samples from breast cancer patients and healthy controls, with efforts taken to address several pitfalls in detection techniques and study design observed in previous studies. In the discovery phase with 122 Caucasian study subjects, we identified 43 microRNAs differentially expressed between breast cancer cases and healthy controls. When those microRNAs were compared with published data from other studies, we identified three microRNAs, including miR-148b, miR-133a and miR-409-3p, whose plasma levels were significantly higher in breast cancer cases than healthy controls and were also significant in previous independent studies. In the validation phase with 50 breast cancer cases and 50 healthy controls, we validated the associations with breast cancer detection for miR-148b and miR-133a (P = 1.5×10-6 and 1.3×10-10, respectively). In the in-vitro study phase, we found that both miR-148b and miR-133a were secreted from breast cancer cell lines, showing their secretory potential and possible tumor origin. Thus, our data suggest that both miR-148b and miR-133a have potential use as biomarkers for breast cancer detection.
authors: Department of Epidemiology, the University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors: Jie
authors: J
authors: Shen
authors: Department of Biostatistics & Bioinformatics, Roswell Park Cancer Institute, Buffalo, NY, USA; Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, P.R.China.
authors: Qiang
authors: Q
authors: Hu
authors: Department of Gynecology and Obstetrics, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany.
authors: Michael
authors: M
authors: Schrauder
authors: Department of Biostatistics & Bioinformatics, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Li
authors: L
authors: Yan
authors: Department of Biostatistics & Bioinformatics, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Dan
authors: D
authors: Wang
authors: Department of Cancer Prevention and Control, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Leonardo
authors: L
authors: Medico
authors: Department of Cancer Prevention and Control, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Yuqing
authors: Y
authors: Guo
authors: Department of Cancer Prevention and Control, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Song
authors: S
authors: Yao
authors: Department of Biostatistics & Bioinformatics, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Qianqian
authors: Q
authors: Zhu
authors: Department of Biostatistics & Bioinformatics, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Biao
authors: B
authors: Liu
authors: Department of Biostatistics & Bioinformatics, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Maochun
authors: M
authors: Qin
authors: Department of Gynecology and Obstetrics, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany.
authors: Matthias W
authors: MW
authors: Beckmann
authors: Department of Gynecology and Obstetrics, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany.
authors: Peter A
authors: PA
authors: Fasching
authors: Department of Gynecology and Obstetrics, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany.
authors: Reiner
authors: R
authors: Strick
authors: Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Candace S
authors: CS
authors: Johnson
authors: Department of Cancer Prevention and Control, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Christine B
authors: CB
authors: Ambrosone
authors: Department of Epidemiology, the University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors: Hua
authors: H
authors: Zhao
authors: Department of Biostatistics & Bioinformatics, Roswell Park Cancer Institute, Buffalo, NY, USA.
authors: Song
authors: S
authors: Liu
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C548234:MIRN133 microRNA, human; C550570:MIRN148 microRNA, human; D035683:MicroRNAs