title: Silence of STIM1 attenuates the proliferation and migration of EPCs after vascular injury and its mechanism.
abstract: OBJECTIVE
  To investigate the effect of stromal interaction molecule 1(STIM1) knockdown on the proliferation and migration of endothelial progenitor cells (EPCs) after vascular injury and its mechanism.
  
  
  METHODS
  The rat bone marrow derived EPCs were divided into three groups: adenovirus negative control (group NSC), rat STIM1 adenovirus vector transfection group (group si/rSTIM1) and rat &human recombinant STIM1 adenovirus transfection group (group si/rSTIM1+hSTIM1). The STIM1 expressions in each group were detected by reverse transcription PCR after transfection; the cell proliferation was tested by [(3)H] thymidine incorporation assay ((3)H-TdR); Cell cycle was analyzed by flow cytometry; the cells' migration activity was detected by Boyden assay; Calcium ion concentration was detected by using laser confocal method.
  
  
  RESULTS
  48 h later after transfection, the expression level of STIM1 in si/rSTIM1 cells was significantly lower than that in NSC group (0.21 ± 0.12 vs 1.01 ± 0.01, P<0.05); EPCs that stayed in G1 phase in si/rSTIM1 group [(93.31 ± 0.24)%] were significantly more than that in NSC group [(78.03 ± 0.34)%, P<0.05]; EPCs' migration activity in si/rSTIM1 group (10.03±0.33) was significantly lower than that in NSC group: (32.11 ± 0.54, P<0.05); EPCs calcium ion concentration changes in EPCs in si/rSTIM1 group (38.03 ± 0.13) was significantly lower than that in NSC group (98.11 ± 0.34, P<0.05). While there was no significant difference between si/rSTIM1+hSTIM1 group and NSC group on the four indexes above.
  
  
  CONCLUSIONS
  Silence of STIM1 attenuates EPCs proliferation and migration after vascular injury, by mediating the calcium ion concentration in EPCs.
authors: Affiliated Shanghai East Hospital of Tongji University, Shanghai 200120, China.
authors: Xin-Peng
authors: XP
authors: Cong
authors: Affiliated Shanghai East Hospital of Tongji University, Shanghai 200120, China.
authors: Wen-Hui
authors: WH
authors: Wang
authors: Shanghai Zhoupu Hospital, Shanghai 201318, China.
authors: Xi
authors: X
authors: Zhu
authors: Shanghai Zhoupu Hospital, Shanghai 201318, China.
authors: Can
authors: C
authors: Jin
authors: Shanghai Zhoupu Hospital, Shanghai 201318, China. Electronic address: zpyykjkll@126.com.
authors: Liang
authors: L
authors: Liu
authors: Shanghai Zhoupu Hospital, Shanghai 201318, China. Electronic address: congxinpeng@126.com.
authors: Xin-Min
authors: XM
authors: Li
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D066026:Endothelial Progenitor Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D016193:G1 Phase / Q000235:genetics; D020868:Gene Silencing; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D000071737:Stromal Interaction Molecule 1; D014162:Transfection; D057772:Vascular System Injuries / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C103530:STIM1 protein, human; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1; D002118:Calcium
keywords: Migration; Proliferation; STIM1; Vascular injury; tEPCs