title: Administration of the resveratrol analogues isorhapontigenin and heyneanol-A protects mice hematopoietic cells against irradiation injuries.
abstract: Ionizing radiation (IR) is known not only to cause acute bone marrow (BM) suppression but also to lead to long-term residual hematopoietic injury. These effects have been attributed to IR inducing the generation of reactive oxygen species (ROS) in hematopoietic cells. In this study, we examined if isorhapontigenin and heyneanol-A, two analogues of resveratrol, could mitigate IR-induced BM suppression. The results of cell viability assays, clonogenic assays, and competitive repopulation assays revealed that treatment with these compounds could protect mice BM mononuclear cells (BMMNC), hematopoietic progenitor cells, and hematopoietic stem cells from IR-induced BM suppression. Moreover, the expression of genes related to the endogenous cellular antioxidant system in hematopoietic cells was analyzed. The expression and activity of SOD2 and GPX1 were found to be decreased in irradiated BMMNC, and the application of the resveratrol analogues could ameliorate this damage. Our results suggest that in comparison with resveratrol and isorhapontigenin, treatment with heyneanol-A can protect hematopoietic cells from IR-induced damage to a greater degree; the protective effects of these compounds are probably the result of their antioxidant properties.
authors: Department of Radiation Oncology, Tianjin Union Medical Center, Tianjin 300121, China.
authors: Hui
authors: H
authors: Wang
authors: Department of Breast Cancer Pathology and Research Laboratory, Key Laboratory of Breast Cancer Prevention and Therapy (Ministry of Education), Key Laboratory of Cancer Prevention and Therapy (Tianjin), National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.
authors: Yi-Ling
authors: YL
authors: Yang
authors: Department of Radiation Oncology, Tianjin Union Medical Center, Tianjin 300121, China ; Institute of Radiation Medicine, Peking Union Medical College (PUMC), Tianjin 300192, China.
authors: Heng
authors: H
authors: Zhang
authors: Department of Radiation Oncology, Tianjin Union Medical Center, Tianjin 300121, China.
authors: Hao
authors: H
authors: Yan
authors: Department of Radiation Oncology, Tianjin Union Medical Center, Tianjin 300121, China.
authors: Xiao-Jing
authors: XJ
authors: Wu
authors: Department of Anorectal Surgery, Tianjin Union Medical Center, Tianjin 300121, China.
authors: Chun-Ze
authors: CZ
authors: Zhang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D019070:Cell Lineage / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003114:Colony-Forming Units Assay; D019610:Cytoprotection / Q000187:drug effects* / Q000528:radiation effects*; D005434:Flow Cytometry; D005979:Glutathione Peroxidase / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011839:Radiation, Ionizing; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
chemical_list: D005979:Glutathione Peroxidase; D000077185:Resveratrol; D013267:Stilbenes; D013482:Superoxide Dismutase; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2; C482862:heyneanol A; C432307:isorhapontigenin; C000722068:Gpx1 protein, mouse