title: Gene expression profiles induced by growth factors in in vitro cultured osteoblasts.
abstract: OBJECTIVE
  Effects of insulin-like growth factor 1 (IGF1), fibroblast growth factor 2 (FGF2) and bone morphogenetic protein 2 (BMP2) on the expression of genes involved in the proliferation and differentiation of osteoblasts in culture were analysed. The best sequence of growth factor addition that induces expansion of cells before their differentiation was sought.
  
  
  METHODS
  Primary human osteoblasts in in vitro culture were treated with IGF1, BMP2 or FGF2 (10 ng/ml) for 24 hours (IGF1) or 48 hours (BMP2 and FGF2). Experiments were performed during the exponential growth phase with approximately 1e7 cells per 75 cm(2) flask. mRNA was reverse transcribed directly and analysed using RT-PCR Taqman assays. Expression levels of key genes involved in cell growth and differentiation (CDH11, TNFRSF11B, RUNX2, POSTN, ALP, WNT5A, LEF1, HSPA5, FOS, p21) were monitored using RT-PCR with gene-specific Taqman probes.
  
  
  RESULTS
  Autocrine expression of BMP2 is stimulated by FGF2 and BMP2 itself. BMP2 and FGF2 act as proliferative factors as indicated by reduced expression of ALP and POSTN, whereas IGF1 exhibits a more subtle picture: the Wingless und Int-1 (Wnt) signalling pathway and the Smad pathway, but not p38 mitogen-activated protein (MAP) kinase signalling, were shown to be activated by IGF1, leading to proliferation and differentiation of the cells.
  
  
  CONCLUSIONS
  For future use of autologous bone cells in the management of bony defects, new treatment options take advantage of growth factors and differentiation factors. Thus, our results might help to guide the timely application of these factors for the expansion and subsequent differentiation of osteoblastic cells in culture. Cite this article: Bone Joint Res 2014;3:236-40.
authors: Donauspital, Department of Laboratory Medicine, Langobardenstr. 122, 1220 Wien, Austria.
authors: A
authors: A
authors: Robubi
authors: Donauspital, Department of Orthopaedics, Langobardenstr. 122, 1220 Wien, Austria.
authors: C
authors: C
authors: Berger
authors: Donauspital, Department of Laboratory Medicine, Langobardenstr. 122, 1220 Wien, Austria.
authors: M
authors: M
authors: Schmid
authors: Donauspital, Department of Laboratory Medicine, Langobardenstr. 122, 1220 Wien, Austria.
authors: K R
authors: KR
authors: Huber
authors: Donauspital, Department of Orthopaedics, Langobardenstr. 122, 1220 Wien, Austria.
authors: A
authors: A
authors: Engel
authors: Donauspital, Department of Laboratory Medicine, Langobardenstr. 122, 1220 Wien, Austria.
authors: W
authors: W
authors: Krugluger
keywords: Alkaline phosphatase; Osteoblasts; SMAD; Tissue engineering; Wnt