title: Steviol glycoside rebaudioside A induces glucagon-like peptide-1 and peptide YY release in a porcine ex vivo intestinal model.
abstract: Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) are hormones important for satiation and are involved in the process called "ileal brake". The aim of this study was to investigate the GLP-1- and PYY-stimulating efficacy of rebaudioside A, casein, and sucrose. This was studied using tissue segments collected from various regions of the pig small intestine. GLP-1 release was strongest from the distal ileum. There, control release was 0.06 ± 0.01 (GLP-1) and 0.07 ± 0.01 (PYY) pmol/cm(2) of tissue. Rebaudioside A (2.5, 12.5, and 25 mM) stimulated GLP-1 release (0.14 ± 0.02, 0.16 ± 0.02, and 0.13 ± 0.02 pmol/cm(2) of tissue, p < 0.001) and PYY release (0.19 ± 0.02, 0.42 ± 0.06, and 0.27 ± 0.03 pmol/cm(2) of tissue, p < 0.001). Sucrose stimulated GLP-1 release (0.08 ± 0.01 pmol/cm(2) of tissue, p < 0.05) only at 10 mM. Casein (0.5%, 1%, and 2.5%, w/v) stimulated GLP-1 release (0.15 ± 0.03, 0.13 ± 0.02, and 0.14 ± 0.01 pmol/cm(2) of tissue, p < 0.001) and PYY release (0.13 ± 0.02, 0.20 ± 0.03, and 0.27 ± 0.03 pmol/cm(2) of tissue, p < 0.01). These findings may help in developing dietary approaches for weight management.
authors: Top Institute Food and Nutrition , Nieuwe Kanaal 9A, 6709 PA Wageningen, The Netherlands.
authors: Dina
authors: D
authors: Ripken
authors: Nikkie
authors: N
authors: van der Wielen
authors: Heleen M
authors: HM
authors: Wortelboer
authors: Jocelijn
authors: J
authors: Meijerink
authors: Renger F
authors: RF
authors: Witkamp
authors: Henk F J
authors: HF
authors: Hendriks
mesh_terms: D000818:Animals; D045786:Diterpenes, Kaurane / Q000378:metabolism*; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism*; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000378:metabolism*; D008954:Models, Biological; D019894:Peptide YY / Q000378:metabolism; D013552:Swine
chemical_list: D045786:Diterpenes, Kaurane; D019894:Peptide YY; C012042:steviol; D052216:Glucagon-Like Peptide 1; C025474:rebaudioside A
keywords: GLP-1; PYY; ex vivo intestinal porcine model; rebaudioside A