title: Using microfluidics to investigate hematopoietic stem cell and microniche interactions at the single cell level.
abstract: In recent years, microfluidic devices have become widely used in biology, and with the advantage of requiring low sample volumes, enables previously technologically infeasible experiments in hematopoietic stem cell (HSC) research. Here, we introduce a microfluidic device to investigate dynamic interactions between HSC and model niches in vitro. The device comprises a pneumatic valve which enables the culturing of different types of niche cells in different parts of the same device. Single HSCs can then be injected into the microfluidic device, manipulated, and placed onto different niches within the same device as controlled by the user. Here, we describe the device fabrication method, the HSC collection methodology, and the operational procedure for the device.
authors: Division of Hem/Onc/BMT, Department of Pediatrics, Aflac Cancer and Blood Disorders Center, Emory University School of Medicine, 2015 Uppergate Drive, ECC 448, Atlanta, 30322, GA, USA.
authors: Byungwook
authors: B
authors: Ahn
authors: Zhengqi
authors: Z
authors: Wang
authors: David R
authors: DR
authors: Archer
authors: Wilbur A
authors: WA
authors: Lam
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D002450:Cell Communication; D002469:Cell Separation; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D007267:Injections; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D011312:Pressure; D059010:Single-Cell Analysis / Q000379:methods*; D055153:Stem Cell Niche
chemical_list: D004129:Dimethylpolysiloxanes; D008567:Membranes, Artificial; C013830:baysilon