title: Growth factors have a protective effect on neomycin-induced hair cell loss.
abstract: We have demonstrated that selected growth factors are involved in regulating survival and proliferation of progenitor cells derived from the neonatal rat organ of Corti (OC). The protective and regenerative effects of these defined growth factors on the injured organ of Corti were therefore investigated. The organ of Corti dissected from the Wistar rat pups (P3-P5) was split into apical, middle, and basal parts, explanted and cultured with or without neomycin and growth factors. Insulin-like growth factor-1 (IGF-1), fibroblast growth factor-2 (FGF-2), and epidermal growth factor (EGF) protected the inner hair cells (IHCs) and outer hair cells (OHCs) from neomycin ototoxicity. Using EGF, IGF-1, and FGF-2 alone induced no protective effect on the survival of auditory hair cells. Combining 2 growth factors (EGF + IGF-1, EGF + FGF-2, or IGF-1 + FGF-2) gave statistically protective effects. Similarly, combining all three growth factors effectively protected auditory hair cells from the ototoxic insult. None of the growth factors induced regeneration of hair cells in the explants injured with neomycin. Thus various combinations of the three defined factors (IGF-1, FGF-2, and EGF) can protect the auditory hair cells from the neomycin-induced ototoxic damage, but no regeneration was seen. This offers a possible novel approach to the treatment of hearing loss.
authors: Department of Bioengineering, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Renmin Road, Shanghai, 201620, China.
authors: Xiangxin
authors: X
authors: Lou
authors: Huihua
authors: H
authors: Yuan
authors: Jing
authors: J
authors: Xie
authors: Xianliu
authors: X
authors: Wang
authors: Liangliang
authors: L
authors: Yang
authors: Yanzhong
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006198:Hair Cells, Auditory / Q000166:cytology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D009355:Neomycin / Q000633:toxicity*; D009925:Organ of Corti / Q000166:cytology / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D020011:Protective Agents; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D009355:Neomycin
keywords: growth factors; hair cells; neomycin; ototoxicity; protection