title: Molecular signalling of a novel curcumin derivative versus Tadalafil in erectile dysfunction.
abstract: The efficacy of a novel curcumin derivative (NCD) versus tadalafil in erectile signalling was assessed. Ten control male rats and 50 diabetic male rats were used and divided into the following: diabetic (DM), curcumin (CURC), NCD, tadalafil and NCD combined with tadalafil rat groups. Cavernous tissue gene expression of heme oxygenase-1 (HO-1), Nrf2, NF-B and p38, enzyme activities of heme oxygenase (HO) and nitric oxide synthase (NOS), cGMP and intracavernosal pressure (ICP)/mean arterial pressure (MAP) were assessed. Results showed that 12 weeks after induction of diabetes, erectile dysfunction (ED) was confirmed by the significant decrease in ICP/MAP, a significant decrease in cGMP, NOS, HO enzyme activities, a significant decrease in HO-1 gene and a significant increase in NF-Ҡβ, p38 genes. Administration of all therapeutic interventions led to a significant increase in ICP/MAP, cGMP levels, a significant increase in HO-1 and NOS enzymes, a significant increase in HO-1, and Nrf2 gene expression, and a significant decrease in NF-Ҡβ, p38 gene expression. NCD or its combination with tadalafil showed significant superiority and more prolonged duration of action. In conclusion, a tendency was observed that CURC and NCD have high efficacy and more prolonged duration of action in enhancing erectile function.
authors: Medical Biochemistry Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors: M T
authors: MT
authors: Abdel Aziz
authors: Medical Biochemistry Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors: A M
authors: AM
authors: Rezq
authors: Medical Biochemistry Department, Faculty of Medicine, Cairo University, Cairo, Egypt.|Clinical Biochemistry Department, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors: H M
authors: HM
authors: Atta
authors: Medical Biochemistry Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors: H
authors: H
authors: Fouad
authors: Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
authors: A M
authors: AM
authors: Zaahkouk
authors: Medical Biochemistry Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors: H H
authors: HH
authors: Ahmed
authors: Medical Biochemistry Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors: D
authors: D
authors: Sabry
authors: Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
authors: H M
authors: HM
authors: Yehia
mesh_terms: D000818:Animals; D003474:Curcumin / Q000031:analogs & derivatives*; D006152:Cyclic GMP / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D007172:Erectile Dysfunction / Q000188:drug therapy* / Q000209:etiology; D051547:Heme Oxygenase-1 / Q000378:metabolism; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000032:analysis; D016328:NF-kappa B / Q000032:analysis; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010413:Penis / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D058986:Phosphodiesterase 5 Inhibitors / Q000627:therapeutic use*; D051381:Rats; D000068581:Tadalafil / Q000627:therapeutic use*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495637:Nfe2l2 protein, rat; D058986:Phosphodiesterase 5 Inhibitors; D000068581:Tadalafil; D019001:Nitric Oxide Synthase; D051547:Heme Oxygenase-1; D048051:p38 Mitogen-Activated Protein Kinases; D006152:Cyclic GMP; D003474:Curcumin
keywords: HO-1; NOS; cGMP; curcumin; erectile dysfunction; intracavernosal pressure