title: Evaluation of the contribution of methylenetetrahydrofolate reductase genotypes to Taiwan breast cancer.
abstract: The aim of the present study was to evaluate the effects of the genotypic polymorphisms in methylenetetrahydrofolate reductase (MTHFR) and its interaction with early-onset breast cancer risk in Taiwan. Two well-known polymorphic variants of MTHFR, C677T (rs1801133) and A1298C (rs1801131), were analyzed and their joint effects with individual age- and estrogen-related factors on breast cancer risk were discussed. In total, 1,232 patients with breast cancer and 1,232 healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The MTHFR C677T genotype, but not the A1298C, was differently distributed between cancer and control groups. The T allele of MTHFR C677T was significantly more frequently found in controls than in patients with cancer. In addition, females carrying MTHFR C677T CT or TT genotypes had a higher odds ratio of 1.21 (95% confidence interval=1.03-1.42, p=1.85E-5) for breast cancer, especially before the age of 45.4 years (odds ratio=1.51 and 95% confidence interval=1.20-1.90). Our results indicate that MTHFR C677T T allele was associated with increased risk of breast cancer in Taiwan, especially in cases who were 45.4 old or younger and with earlier menarche age (<12.2 years).
authors: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, R.O.C. Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.
authors: Chung-Yu
authors: CY
authors: Huang
authors: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C. Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Wen-Shin
authors: WS
authors: Chang
authors: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, R.O.C.
authors: Hao-Ai
authors: HA
authors: Shui
authors: Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.
authors: Yung-Hung
authors: YH
authors: Hsieh
authors: Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.
authors: Ching-Hui
authors: CH
authors: Loh
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Hwei-Chung
authors: HC
authors: Wang
authors: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C. Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Hong-Xue
authors: HX
authors: Ji
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Chieh-Lun
authors: CL
authors: Hsiao
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Chin-Mu
authors: CM
authors: Hsu
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, R.O.C.
authors: Chia-Wen
authors: CW
authors: Tsai
authors: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C. Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, R.O.C. artbau1@yahoo.com.tw artbau2@gmail.com.
authors: Da-Tian
authors: DT
authors: Bau
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000209:etiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D013624:Taiwan
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: Breast cancer; MTHFR; Taiwan; carcinogenesis; genotype; polymorphism