title: Liver X receptor agonist prevents LPS-induced mastitis in mice.
abstract: Liver X receptor-α (LXR-α) which belongs to the nuclear receptor superfamily, is a ligand-activated transcription factor. Best known for its ability to regulate lipid metabolism and transport, LXRs have recently also been implicated in regulation of inflammatory response. The aim of this study was to investigate the preventive effects of synthetic LXR-α agonist T0901317 on LPS-induced mastitis in mice. The mouse model of mastitis was induced by injection of LPS through the duct of mammary gland. T0901317 was injected 1h before and 12h after induction of LPS intraperitoneally. The results showed that T0901317 significantly attenuated the infiltration of neutrophilic granulocytes, and the activation of myeloperoxidase (MPO); down-regulated the level of pro-inflammatory mediators including TNF-α, IL-1β, IL-6, COX-2 and PEG2; inhibited the phosphorylation of IκB-α and NF-κB p65, caused by LPS. Moreover, we report for the first time that LXR-α activation impaired LPS-induced mastitis. Taken together, these data indicated that T0901317 had protective effect on mastitis and the anti-inflammatory mechanism of T0901317 on LPS induced mastitis in mice may be due to its ability to inhibit NF-κB signaling pathway. LXR-α activation can be used as a therapeutic approach to treat mastitis.
authors: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, PR China.
authors: Yunhe
authors: Y
authors: Fu
authors: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, PR China.
authors: Yuan
authors: Y
authors: Tian
authors: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, PR China.
authors: Zhengkai
authors: Z
authors: Wei
authors: Daqing Honggang District Animal Health Supervision, Daqing, Heilongjiang Province 163000, PR China.
authors: Hui
authors: H
authors: Liu
authors: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, PR China.
authors: Xiaojing
authors: X
authors: Song
authors: Daqing Honggang District Animal Health Supervision, Daqing, Heilongjiang Province 163000, PR China.
authors: Wenbo
authors: W
authors: Liu
authors: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, PR China.
authors: Wenlong
authors: W
authors: Zhang
authors: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, PR China.
authors: Wei
authors: W
authors: Wang
authors: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, PR China.
authors: Yongguo
authors: Y
authors: Cao
authors: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, PR China. Electronic address: zhangns61@sohu.com.
authors: Naisheng
authors: N
authors: Zhang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051546:Cyclooxygenase 2 / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D015232:Dinoprostone / Q000276:immunology; D005260:Female; D006845:Hydrocarbons, Fluorinated / Q000494:pharmacology / Q000627:therapeutic use*; D020672:I-kappa B Proteins / Q000276:immunology; D008070:Lipopolysaccharides; D000071518:Liver X Receptors; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008413:Mastitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D008807:Mice, Inbred BALB C; D000072000:NF-KappaB Inhibitor alpha; D057093:Orphan Nuclear Receptors / Q000819:agonists*; D009195:Peroxidase / Q000276:immunology; D013449:Sulfonamides / Q000494:pharmacology / Q000627:therapeutic use*; D051996:Transcription Factor RelA / Q000276:immunology; D005466:Fluorocarbons; D000096926:Benzenesulfonamides
chemical_list: D000893:Anti-Inflammatory Agents; D051546:Cyclooxygenase 2; D016207:Cytokines; D015232:Dinoprostone; D006845:Hydrocarbons, Fluorinated; D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; D000071518:Liver X Receptors; D000072000:NF-KappaB Inhibitor alpha; D057093:Orphan Nuclear Receptors; D009195:Peroxidase; D013449:Sulfonamides; D051996:Transcription Factor RelA; C000606205:Nfkbia protein, mouse; C000605214:Nr1h3 protein, mouse; C423915:T0901317; D005466:Fluorocarbons; D000096926:Benzenesulfonamides; C077623:Ptgs2 protein, mouse
keywords: LXR-α; Mastitis; Myeloperoxidase; Nuclear factor-kappaB (NF-κB)