title: Effects of naproxen on cell proliferation and genotoxicity in MG-63 osteosarcoma cell line.
abstract: The purpose of this study was to determine the efficacy of naproxen, a nonsteroidal anti-inflammatory drug, on the MG-63 human osteosarcoma cell line. MG-63 cells were exposed to naproxen in a wide range of concentrations of 0.03, 0.05, 0.1, 0.42, 0.83, and 1.67 mg/ml for 72 h. The activity of naproxen was assessed by the following assays: cell morphology; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method; terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay; comet assay; and acridine orange and monodansylcadaverine (MDC) staining. Naproxen exerted a significant inhibitory effect on MG-63 cell proliferation, in a concentration-dependent manner, in all treatment groups compared with untreated cells. An increase in frequency of DNA damage, apoptotic bodies, apoptotic cells, and autophagic vacuoles was observed in MG-63-treated cells. Although future studies are needed, these findings suggest that naproxen may lead to improvements in treatment of patients with osteosarcoma.
authors: a Department of Genetics and Biotechnology and CECAV , University of Trás-os-Montes and Alto Douro , Vila Real , Portugal.
authors: Isabel
authors: I
authors: Correia
authors: Regina
authors: R
authors: Arantes-Rodrigues
authors: Rosário
authors: R
authors: Pinto-Leite
authors: Isabel
authors: I
authors: Gaivão
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D020287:In Situ Nick-End Labeling; D009288:Naproxen / Q000494:pharmacology*; D012516:Osteosarcoma / Q000473:pathology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D009288:Naproxen