title: Puerarin facilitates T-tubule development of murine embryonic stem cell-derived cardiomyocytes.
abstract: OBJECTIVE
  The embryonic stem cell-derived cardiomyocytes (ES-CM) is one of the promising cell sources for repopulation of damaged myocardium. However, ES-CMs present immature structure, which impairs their integration with host tissue and functional regeneration. This study used murine ES-CMs as an in vitro model of cardiomyogenesis to elucidate the effect of puerarin, the main compound found in the traditional Chinese medicine the herb Radix puerariae, on t-tubule development of murine ES-CMs.
  
  
  METHODS
  Electron microscope was employed to examine the ultrastructure. The investigation of transverse-tubules (t-tubules) was performed by Di-8-ANEPPS staining. Quantitative real-time PCR was utilized to study the transcript level of genes related to t-tubule development.
  
  
  RESULTS
  We found that long-term application of puerarin throughout cardiac differentiation improved myofibril array and sarcomeres formation, and significantly facilitated t-tubules development of ES-CMs. The transcript levels of caveolin-3, amphiphysin-2 and junctophinlin-2, which are crucial for the formation and development of t-tubules, were significantly upregulated by puerarin treatment. Furthermore, puerarin repressed the expression of miR-22, which targets to caveolin-3.
  
  
  CONCLUSIONS
  Our data showed that puerarin facilitates t-tubule development of murine ES-CMs. This might be related to the repression of miR-22 by puerarin and upregulation of Cav3, Bin1 and JP2 transcripts.
authors: Department of Physiology and Chinese-German Stem Cell Center, Tongji Medical College, Huazhong University of Science and Technology, The Key Laboratory for Drug Target Researches and Pharmacodynamic Evaluation of Hubei Province, Wuhan, China.
authors: Lu
authors: L
authors: Wang
authors: Yurong
authors: Y
authors: Cui
authors: Ming
authors: M
authors: Tang
authors: Xinwu
authors: X
authors: Hu
authors: Hongyan
authors: H
authors: Luo
authors: Jürgen
authors: J
authors: Hescheler
authors: Jiaoya
authors: J
authors: Xi
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D051244:Caveolin 3 / Q000235:genetics; D002454:Cell Differentiation; D017931:DNA Primers; D053595:Embryonic Stem Cells / Q000166:cytology*; D007529:Isoflavones / Q000494:pharmacology*; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D035683:MicroRNAs / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D009124:Muscle Proteins / Q000235:genetics; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051244:Caveolin 3; D017931:DNA Primers; D007529:Isoflavones; D008565:Membrane Proteins; D035683:MicroRNAs; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; C109221:Bin1 protein, mouse; C574876:Mirn22 microRNA, mouse; C000589042:JP2 protein, mouse; C033607:puerarin