title: The effects of hypokalaemia on the hormone exocytosis in adenohypophysis and prolactinoma cell culture model systems.
abstract: The extracellular ion milieu determines the exocytosis mechanism that is coupled to spontaneous electrical activity. The K(+) ion plays crucial role in this mechanism: as the potassium current is associated with membrane hyperpolarization and hormone release through protein cascade activation. The primary aim of this study was to investigate the response mechanisms of normal adenohypophysis and adenohypophyseal prolactinoma cell populations at different extracellular K(+) levels with an otherwise isoionic milieu of all other essential ions. We focused on prolactin (PRL) and adrenocorticotrophic hormone (ACTH) release.In our experimental study, female Wistar rats (n=20) were treated with estrone-acetate (150 μg/kg b.w./week) for 6 months to induce prolactinomas in the adenohypophysis. Primary, monolayer cell cultures were prepared by enzymatic and mechanical digestion. PRL and ACTH hormone presence was measured by radioimmunoassay or immuno-chemiluminescence assay. Immunocytochemistry was used to assess the apoptotic cells.Differences between the effects of hypokalaemia on normal adenohypophysis cultures and prolactinoma cell populations were investigated. Significant alteration (p<0.001, n=10) in hormone exocytosis was detected in K(+) treated adenohypophyseal and prolactinoma cell cultures compared to untreated groups. Immunocyto-chemistry showed that Bcl-2 expression was reduced under hypokalaemic conditions.The decrease in hormone exocytosis was tightly correlated to the extracellular K(+) in both cell types, leading to the conclusion that external K(+) may be the major factor for the inhibition of hormone release. The significant increase in hormone content in supernatant media suggests that hypokalaemia may play important role in apoptosis.
authors: Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, Institute of Applied Science,University of Szeged, Szeged, Hungary.
authors: Z
authors: Z
authors: Molnár
authors: Department of Pulmonology, Faculty of Medicine, University of Szeged, Szeged, Hungary.
authors: R
authors: R
authors: Pálföldi
authors: Department of Medical Physics and Informatics, Faculty of Medicine, -University of Szeged, Szeged, Hungary.
authors: A
authors: A
authors: László
authors: Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, Institute of Applied Science,University of Szeged, Szeged, Hungary.
authors: M
authors: M
authors: Radács
authors: Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, Institute of Applied Science,University of Szeged, Szeged, Hungary.
authors: M
authors: M
authors: László
authors: Invasive Cardiology Department, Second Department of Internal Medicine and Cardiology, Faculty of Medicine, University of Szeged, Szeged, Hungary.
authors: P
authors: P
authors: Hausinger
authors: Department of Pathology, Faculty of Medicine, University of Szeged, Szeged, Hungary.
authors: L
authors: L
authors: Tiszlavicz
authors: Department of Pathology, Faculty of Medicine, University of Szeged, Szeged, Hungary.
authors: Z
authors: Z
authors: Rázga
authors: Endocrine Unit of First Department of Internal Medicine, Faculty of -Medicine, University of Szeged, Szeged, Hungary.
authors: Z
authors: Z
authors: Valkusz
authors: Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, Institute of Applied Science,University of Szeged, Szeged, Hungary.
authors: M
authors: M
authors: Gálfi
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004970:Estrone / Q000031:analogs & derivatives; D005089:Exocytosis / Q000187:drug effects*; D005260:Female; D007008:Hypokalemia / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D011188:Potassium / Q000494:pharmacology*; D011388:Prolactin / Q000378:metabolism*; D015175:Prolactinoma / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004970:Estrone; C014945:acetoxyestrone; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D011188:Potassium