title: Helicobacter pylori is not eradicated after triple therapy: a nested PCR based study.
abstract: Detection of Helicobacter pylori after triple therapy is usually carried out by either rapid urease test (RUT), urea breath test (UBT), histology, bacterial isolation, and single round PCR or serological tests. In this study, antral biopsy specimens from 25 patients were tested for H. pylori by RUT, culture, histology, and nested PCR in their antral biopsy specimens before and after treatment. Three genes, namely, heat shock protein (hsp60), phosphoglucosamine mutase (ureC), and flagellar export ATP synthase (fliI) of H. pylori were targeted. Of the 25 antral biopsy specimens, the RUT, culture, histology, and nested PCR positivity dropped from 81.8% to 12%, 31% to 0%, 100 to 84%, and 100% to 92%, respectively, before and after therapy. Further, hsp60 specific amplicons from 23 out of 25 patients gave identical restriction pattern, while 6 fliI and 1 ureC specific amplicon produced different restriction pattern. Furthermore, variations in fliI gene sequences in H. pylori after treatment were also confirmed by sequencing and compared in silico. Nested PCR based detection of H. pylori is more sensitive method to detect H. pylori after therapy than culture, RUT, and histology. Further, this study suggests that H. pylori is not eradicated completely after triple therapy.
authors: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
authors: Saurabh Kumar
authors: 0000-0001-9472-0188
authors: SK
authors: Patel
authors: Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
authors: Girish Narayan
authors: GN
authors: Mishra
authors: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
authors: Chandra Bhan
authors: 0000-0002-1101-3689
authors: CB
authors: Pratap
authors: Department of Gastroenterology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
authors: Ashok Kumar
authors: AK
authors: Jain
authors: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
authors: Gopal
authors: G
authors: Nath
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000658:Amoxicillin / Q000008:administration & dosage; D001426:Bacterial Proteins / Q000235:genetics; D001706:Biopsy; D001944:Breath Tests; D018834:Chaperonin 60 / Q000235:genetics; D017291:Clarithromycin / Q000008:administration & dosage; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009853:Omeprazole / Q000008:administration & dosage; D010733:Phosphoglucomutase / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000235:genetics; D014510:Urease / Q000378:metabolism; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; C064971:FliA protein, Bacteria; D012808:Sigma Factor; D000658:Amoxicillin; D000255:Adenosine Triphosphate; D014510:Urease; C097631:phosphoglucosamine mutase; D010733:Phosphoglucomutase; D017291:Clarithromycin; D009853:Omeprazole