title: [Changes of biological behavioral of E. coli K1 after ppk1 gene deletion].
abstract: OBJECTIVE
  To study the changes in biological behaviors of meningitis E. coli K1 strain E44 after deletion of polyphosphate kinase 1 (ppk1) gene and explore the role of ppk1 in the pathogenesis of E. coli K1-induced meningitis.
  
  
  METHODS
  The wild-type strain E. coli K1 and ppk1 deletion mutant were exposed to heat at 56 degrees celsius; for 6 min, and their survival rates were determined. The adhesion and invasion of the bacteria to human brain microvascular endothelial cells (HBMECs) were observed using electron microscopy and quantitative tests. HBMECs were co-incubated with wild-type strain or ppk1 deletion mutant, and the cytoskeleton rearrangement was observed under laser scanning confocal microscope.
  
  
  RESULTS
  The survival rate of the ppk1 deletion mutant was significantly lower than that of the wild-type strain after heat exposure. The ppk1 deletion mutant also showed lowered cell adhesion and invasion abilities and weakened ability to induce cytoskeleton rearrangement in HBMECs.
  
  
  CONCLUSIONS
  ppk1 gene is important for E.coli K1 for heat resistance, cell adhesion and invasion, and for inducing cytoskeletal rearrangement in HBMECs.
authors: Clinical Laboratory, Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China. E-mail: pl_206@126.com.
authors: Liang
authors: L
authors: Peng
authors: Jiayun
authors: J
authors: Pan
authors: Su
authors: S
authors: Luo
authors: Zhenghui
authors: Z
authors: Yang
authors: Mufang
authors: M
authors: Huang
authors: Hong
authors: H
authors: Cao
mesh_terms: D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D003599:Cytoskeleton; D042783:Endothelial Cells / Q000166:cytology / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D017353:Gene Deletion; D006801:Humans; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase