title: A novel electrospun-aligned nanoyarn-reinforced nanofibrous scaffold for tendon tissue engineering.
abstract: An electrospun-aligned nanoyarn-reinforced nanofibrous scaffold (NRS) was developed for tendon tissue engineering to improve mechanical strength and cell infiltration. The novel scaffold composed of aligned nanoyarns and random nanofibers was fabricated via electrospinning using a two-collector system. The aim of the present study was to investigate three different types of electrospun scaffolds (random nanofibrous scaffold, aligned nanofibrous scaffold and NRS) based on silk fibroin (SF) and poly(l-lactide-co-caprolactone) blends. Morphological analysis demonstrated that the NRS composed of aligned nanoyarns and randomly distributed nanofibers formed a 3D microstructure with relatively large pore sizes and high porosity. Biocompatibility analysis revealed that bone marrow-derived mesenchymal stem cells exhibited a higher proliferation rate when cultured on the NRS compared with the other scaffolds. The mechanical testing results indicated that the tensile properties of the NRS were reinforced in the direction parallel to the nanoyarns and satisfied the mechanical requirements for tendon repair. In addition, cell infiltration was significantly enhanced on the NRS. In conclusion, with its improved porosity and appropriate mechanical properties, the developed NRS shows promise for tendon tissue engineering applications.
authors: Department of Orthopaedics, Changzheng Hospital affiliated with Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China; Department of Spinal Surgery, Lanzhou General Hospital of Lanzhou Military Command Region, 333 Nanbinhe Road, Lanzhou 730050, PR China.
authors: Chengwei
authors: C
authors: Yang
authors: Department of Orthopaedics, Changzheng Hospital affiliated with Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China.
authors: Guoying
authors: G
authors: Deng
authors: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 Renmin Road North, Songjiang District, Shanghai 201620, PR China.
authors: Weiming
authors: W
authors: Chen
authors: Department of Orthopaedics, Changzheng Hospital affiliated with Second Military Medical University, 415 Fengyang Road, Shanghai 200003, PR China. Electronic address: xjye2012@gmail.com.
authors: Xiaojian
authors: X
authors: Ye
authors: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 Renmin Road North, Songjiang District, Shanghai 201620, PR China. Electronic address: xmm@dhu.edu.cn.
authors: Xiumei
authors: X
authors: Mo
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D049329:Nanostructures; D051381:Rats; D017207:Rats, Sprague-Dawley; D013710:Tendons / Q000254:growth & development*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials
keywords: Cell infiltration; Electrospinning; Nanoyarn; Tendon tissue engineering; Three-dimensional scaffold