title: Photopatterned antibodies for selective cell attachment.
abstract: We present a phototriggerable system that allows for the spatiotemporal controlled attachment of selected cell types to a biomaterial using immobilized antibodies that specifically target individual cell phenotypes. o-Nitrobenzyl caged biotin was used to functionalize chitosan membranes and mediate site-specific coupling of streptavidin and biotinylated antibodies after light activation. The ability of this system to capture and immobilize specific cells on a surface was tested using endothelial-specific biotinylated antibodies and nonspecific ones as controls. Homogeneous patterned monolayers of human umbilical vein endothelial cells were obtained on CD31-functionalized surfaces. This is a simple and generic approach that is applicable to other ligands, materials, and cell types and shows the flexibility of caged ligands to trigger and control the interaction between cells and biomaterials.
authors: Max-Planck-Institut für Polymerforschung , Ackermannweg 10, 55128 Mainz, Germany.
authors: C A
authors: CA
authors: Custódio
authors: V
authors: V
authors: San Miguel-Arranz
authors: R A
authors: RA
authors: Gropeanu
authors: M
authors: M
authors: Gropeanu
authors: M
authors: M
authors: Wirkner
authors: R L
authors: RL
authors: Reis
authors: J F
authors: JF
authors: Mano
authors: A
authors: A
authors: del Campo
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D006801:Humans; D019809:Streptavidin / Q000737:chemistry
chemical_list: D000906:Antibodies; D055910:Antibodies, Immobilized; D001710:Biotin; D019809:Streptavidin