title: Efficient production of acetone-butanol-ethanol (ABE) from cassava by a fermentation-pervaporation coupled process.
abstract: Production of acetone-butanol-ethanol (ABE) from cassava was investigated with a fermentation-pervaporation (PV) coupled process. ABE products were in situ removed from fermentation broth to alleviate the toxicity of solvent to the Clostridium acetobutylicum DP217. Compared to the batch fermentation without PV, glucose consumption rate and solvent productivity increased by 15% and 21%, respectively, in batch fermentation-PV coupled process, while in continuous fermentation-PV coupled process running for 304 h, the substrate consumption rate, solvent productivity and yield increased by 58%, 81% and 15%, reaching 2.02 g/Lh, 0.76 g/Lh and 0.38 g/g, respectively. Silicalite-1 filled polydimethylsiloxane (PDMS)/polyacrylonitrile (PAN) membrane modules ensured media recycle without significant fouling, steadily generating a highly concentrated ABE solution containing 201.8 g/L ABE with 122.4 g/L butanol. After phase separation, a final product containing 574.3g/L ABE with 501.1g/L butanol was obtained. Therefore, the fermentation-PV coupled process has the potential to decrease the cost in ABE production.
authors: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China; College of Biology Science & Engineering, Hebei University of Economics & Business, Shijiazhuang 050061, PR China.
authors: Jing
authors: J
authors: Li
authors: University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Xiangrong
authors: X
authors: Chen
authors: University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Benkun
authors: B
authors: Qi
authors: University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Jianquan
authors: J
authors: Luo
authors: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Yuming
authors: Y
authors: Zhang
authors: University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Yi
authors: Y
authors: Su
authors: University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: yhwan@home.ipe.ac.cn.
authors: Yinhua
authors: Y
authors: Wan
mesh_terms: D000096:Acetone / Q000378:metabolism*; D000681:Amylases / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D002244:Carbon / Q000494:pharmacology; D046969:Clostridium acetobutylicum / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D002365:Manihot / Q000187:drug effects / Q000378:metabolism*; D008567:Membranes, Artificial
chemical_list: D000440:Butanols; D008567:Membranes, Artificial; D000096:Acetone; D000431:Ethanol; D002244:Carbon; D000681:Amylases
keywords: Butanol; Cassava; Coupled process; Fermentation; Pervaporation