title: The yeast histone chaperone hif1p functions with RNA in nucleosome assembly.
abstract: BACKGROUND
  Hif1p is an H3/H4-specific histone chaperone that associates with the nuclear form of the Hat1p/Hat2p complex (NuB4 complex) in the yeast Saccharomyces cerevisiae. While not capable of depositing histones onto DNA on its own, Hif1p can act in conjunction with a yeast cytosolic extract to assemble nucleosomes onto a relaxed circular plasmid.
  
  
  RESULTS
  To identify the factor(s) that function with Hif1p to carry out chromatin assembly, multiple steps of column chromatography were carried out to fractionate the yeast cytosolic extract. Analysis of partially purified fractions indicated that Hif1p-dependent chromatin assembly activity resided in RNA rather than protein. Fractionation of isolated RNA indicated that the chromatin assembly activity did not simply purify with bulk RNA. In addition, the RNA-mediated chromatin assembly activity was blocked by mutations in the human homolog of Hif1p, sNASP, that prevent the association of this histone chaperone with histone H3 and H4 without altering its electrostatic properties.
  
  
  CONCLUSIONS
  These results suggest that specific RNA species may function in concert with histone chaperones to assemble chromatin.
authors: Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio, United States of America.
authors: Amy R
authors: AR
authors: Knapp
authors: Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio, United States of America.
authors: Huanyu
authors: H
authors: Wang
authors: Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio, United States of America.
authors: Mark R
authors: MR
authors: Parthun
mesh_terms: D002843:Chromatin / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D056488:Histone Chaperones / Q000235:genetics / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D012313:RNA / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D056488:Histone Chaperones; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA