title: Bioactivity of Meeker and Willamette raspberry (Rubus idaeus L.) pomace extracts.
abstract: Taking into account the substantial potential of raspberry processing by-products, pomace extracts from two raspberry cultivars, Meeker and Willamette, were investigated. Total phenolic, flavonoid and anthocyanin contents were determined. Willamette pomace extract (EC₅₀=0.042 mg/ml) demonstrated stronger 2,2-diphenyl-1-picrylhydrazyl DPPH radical-scavenging activity than did Meeker pomace extract (EC₅₀=0.072 mg/ml). The most pronounced cell growth inhibition effect was obtained in the breast adenocarcinoma cell line, reaching EC50 values of 34.8 and 60.3 μg/ml for Willamette and Meeker extracts, respectively. Both extracts demonstrated favourable non-tumor/tumor cell growth ratios and potently increased the apoptosis/necrosis ratio in breast adenocarcinoma and cervix carcinoma cells. In reference and wild bacterial strains, minimal inhibitory concentrations (MIC) were achieved in a concentration range from 0.29 to 0.59 mg/ml, and minimal bactericidal concentrations (MBC) in a range from 0.39 to 0.78 mg/ml. The results indicate significant antioxidant, antiproliferative, proapoptotic and antibacterial activities of raspberry pomace and favour its use as a functional food ingredient.
authors: University of Novi Sad, Faculty of Medicine, Oncology Institute of Vojvodina, Dr Goldmana 4, 21204 Sremska Kamenica, Serbia. Electronic address: ddaaggeerr@gmail.com.
authors: Dragana D
authors: DD
authors: Četojević-Simin
authors: University of Novi Sad, Faculty of Technology, Bul. Cara Lazara 1, 21000 Novi Sad, Serbia.
authors: Aleksandra S
authors: AS
authors: Velićanski
authors: University of Novi Sad, Faculty of Technology, Bul. Cara Lazara 1, 21000 Novi Sad, Serbia.
authors: Dragoljub D
authors: DD
authors: Cvetković
authors: University of Novi Sad, Faculty of Technology, Bul. Cara Lazara 1, 21000 Novi Sad, Serbia.
authors: Siniša L
authors: SL
authors: Markov
authors: University of Novi Sad, Faculty of Technology, Bul. Cara Lazara 1, 21000 Novi Sad, Serbia.
authors: Gordana S
authors: GS
authors: Ćetković
authors: University of Novi Sad, Faculty of Technology, Bul. Cara Lazara 1, 21000 Novi Sad, Serbia.
authors: Vesna T
authors: VT
authors: Tumbas Šaponjac
authors: University of Novi Sad, Faculty of Technology, Bul. Cara Lazara 1, 21000 Novi Sad, Serbia.
authors: Jelena J
authors: JJ
authors: Vulić
authors: University of Novi Sad, Faculty of Technology, Bul. Cara Lazara 1, 21000 Novi Sad, Serbia.
authors: Jasna M
authors: JM
authors: Čanadanović-Brunet
authors: University of Novi Sad, Faculty of Technology, Bul. Cara Lazara 1, 21000 Novi Sad, Serbia.
authors: Sonja M
authors: SM
authors: Djilas
mesh_terms: D000872:Anthocyanins; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry*; D017209:Apoptosis; D005419:Flavonoids / Q000737:chemistry*; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts / Q000737:chemistry*; D065856:Rubus / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts
keywords: Antibacterial activity; Antitumor activity; Apoptosis; Free radical-scavenging activity; Raspberry pomace