title: Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs.
abstract: Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of cell-cell contact. These cell pairs can be utilized in patch clamp recordings to quantify electrical interactions between cardiomyocytes and non-cardiomyocytes.
authors: Department of Biomedical Engineering, Duke University, 3000 Science Drive, Hudson Hall 136, 90281, Durham, NC, USA.
authors: Hung
authors: H
authors: Nguyen
authors: Nima
authors: N
authors: Badie
authors: Luke
authors: L
authors: McSpadden
authors: Dawn
authors: D
authors: Pedrotty
authors: Nenad
authors: N
authors: Bursac
mesh_terms: D000200:Action Potentials; D002450:Cell Communication; D055724:Electrophysiological Phenomena; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods*