title: A one-tube multiplexed colorimetric strategy based on plasmonic nanoparticles combined with non-negative matrix factorization.
abstract: Herein, a one-tube colorimetric platform has been developed for the simultaneous determination of two analytes (DNA as model object) in one tube with picomolar sensitivity. SPR-active nanoparticles are used to encode reporter probes sensitive to oligonucleotides associated with hepatitis A virus Vall7 polyprotein gene (HVA) and hepatitis B virus surface-antigen gene (HVB) respectively and magnetic beads (MBs) serve as the removal tool. In this mixed nanoparticles based biosensor, the addition of target analytes could change the concentration of each nanoparticle, leading to different colors of the supernatant. The influence of spectral overlap has been eliminated by a non-negative matrix factorization (NMF). With the assistance of NMF, the limit of detection (LOD) can be determinated as pM level without amplification. On the whole, this nanosensor boasts the advantages of high sensitivity and low sample consumption. Simultaneous colorimetric detection and quantification of two molecules in one tube are demonstrated.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Yizhen
authors: Y
authors: Liu
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Wei
authors: W
authors: Fang
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Zitong
authors: Z
authors: Wu
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Guohua
authors: G
authors: Zhou
authors: School of Naval Architecture & Ocean Engineering, Huazhong University of Science and Technology, Wuhan 430072, China.
authors: Wen
authors: W
authors: Yi
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Xiaodong
authors: X
authors: Zhou
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Aiguo
authors: A
authors: Shen
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, China. Electronic address: jmhu@whu.edu.cn.
authors: Jiming
authors: J
authors: Hu
mesh_terms: D000465:Algorithms; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D030041:Hepatitis A virus / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008863:Microspheres; D015345:Oligonucleotide Probes / Q000737:chemistry* / Q000235:genetics; D020815:Polyproteins / Q000235:genetics; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance / Q000379:methods
chemical_list: D006514:Hepatitis B Surface Antigens; D015345:Oligonucleotide Probes; D020815:Polyproteins; D004247:DNA
keywords: Colorimetry; Detection; Nanoparticles; Non-negative matrix factorization; Oligonucleotides