title: The IL-8 protease SpyCEP is detrimental for Group A Streptococcus host-cells interaction and biofilm formation.
abstract: SpyCEP-mediated chemokine degradation translates into more efficient spreading and increased severity of invasive Group A Streptococcus (GAS) infections, due to impaired neutrophil recruitment to the site of infection. SpyCEP is markedly up-regulated in invasive as compared to colonizing GAS isolates raising the question whether SpyCEP expression hinders bacterial attachment and thus colonization of the host. To address this question we used a molecular approach involving the use of homologous GAS strains either expressing or not SpyCEP or expressing an enzymatically inactive variant of SpyCEP. We found that expression of enzymatically functional SpyCEP lowered GAS adherence and invasion potential toward various epithelial and endothelial cells. SpyCEP also blunted biofilm formation capacity. Our data indicate that expression of SpyCEP decreases colonization and thus might be detrimental for the spreading of GAS.
authors: Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich Zurich, Switzerland.
authors: Federica
authors: F
authors: Andreoni
authors: Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich Zurich, Switzerland.
authors: Taiji
authors: T
authors: Ogawa
authors: Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich Zurich, Switzerland.
authors: Mariko
authors: M
authors: Ogawa
authors: Surgical Intensive Care Medicine, University Hospital Zurich, University of Zurich Zurich, Switzerland.
authors: Jerzy
authors: J
authors: Madon
authors: Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich Zurich, Switzerland.
authors: Satoshi
authors: S
authors: Uchiyama
authors: Surgical Intensive Care Medicine, University Hospital Zurich, University of Zurich Zurich, Switzerland.
authors: Reto A
authors: RA
authors: Schuepbach
authors: Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich Zurich, Switzerland.
authors: Annelies S
authors: AS
authors: Zinkernagel
keywords: Group A Streptococcus; IL-8 protease; SpyCEP; biofilms; colonization; virulence factors