title: Living biointerfaces based on non-pathogenic bacteria to direct cell differentiation.
abstract: Genetically modified Lactococcus lactis, non-pathogenic bacteria expressing the FNIII(7-10) fibronectin fragment as a protein membrane have been used to create a living biointerface between synthetic materials and mammalian cells. This FNIII(7-10) fragment comprises the RGD and PHSRN sequences of fibronectin to bind α5β1 integrins and triggers signalling for cell adhesion, spreading and differentiation. We used L. lactis strain to colonize material surfaces and produce stable biofilms presenting the FNIII(7-10) fragment readily available to cells. Biofilm density is easily tunable and remains stable for several days. Murine C2C12 myoblasts seeded over mature biofilms undergo bipolar alignment and form differentiated myotubes, a process triggered by the FNIII(7-10) fragment. This biointerface based on living bacteria can be further modified to express any desired biochemical signal, establishing a new paradigm in biomaterial surface functionalisation for biomedical applications.
authors: 1] Center for Biomaterials and Tissue Engineering, Universitat Politécnica de València, Spain [2] Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom.
authors: Aleixandre
authors: A
authors: Rodrigo-Navarro
authors: Center for Biomaterials and Tissue Engineering, Universitat Politécnica de València, Spain.
authors: Patricia
authors: P
authors: Rico
authors: Abengoa Research, Campus Palmas Altas, Sevilla, Spain.
authors: Anas
authors: A
authors: Saadeddin
authors: Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology, Atlanta, GA 30332, USA.
authors: Andres J
authors: AJ
authors: Garcia
authors: Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom.
authors: Manuel
authors: M
authors: Salmeron-Sanchez
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D018441:Biofilms / Q000254:growth & development*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D005353:Fibronectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D039081:Integrin alpha5beta1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013294:Lactococcus lactis / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D051379:Mice; D008969:Molecular Sequence Data; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D011485:Protein Binding; D015398:Signal Transduction; D054457:Tissue Scaffolds; D019076:Transgenes
chemical_list: D005353:Fibronectins; D039081:Integrin alpha5beta1