title: Use of BRAF V600E as a molecular marker in aggressive colorectal cancer.
abstract: OBJECTIVE
  to compare the immunoexpression of BRAF V600E among stage of colorectal cancer.
  
  
  METHODS
  this was a cross sectional, and retrospective study involving Dukes' stage A, B, and C colorectal carcinoma, each with 15 cases. Immunohistochemistry was performed in paraffin-embedded specimens of tumor mass for the assessment of BRAF V600E. The proportion differences of immunoexpression of BRAF V600E among Dukes' stage A, B, and C were tested using Chi-Square test.
  
  
  RESULTS
  the result of positive BRAF V600E immunoexpression (moderately to strongly positive) in Dukes' stage A, B, and C were found in 1 of 15 cases, 4 of 15 cases and 13 of 15 cases respectively. BRAF V600E immunoexpression was statistically significant more frequent in Dukes' stage C (p<0,001, Chi-Square test).
  
  
  CONCLUSIONS
  positive BRAF V600E immunoexpression could be used as a marker of aggresive colorectal carcinoma.
authors: Department of Anatomical Pathology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
authors: Bethy S
authors: BS
authors: Hernowo
authors: Fenny
authors: F
authors: Ariyanni
authors: Sri
authors: S
authors: Suryanti
authors: Abdul H
authors: AH
authors: Hassan
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology*; D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D048493:Proto-Oncogene Proteins B-raf / Q000032:analysis*; D012189:Retrospective Studies
chemical_list: D048493:Proto-Oncogene Proteins B-raf