title: Antigenotoxic and antimutagenic effects of glutamine supplementation on mice treated with cisplatin.
abstract: We evaluated the effects of glutamine on clastogenic and genotoxic damage prevention caused by the administration of cisplatin. Forty Swiss mice were divided into 8 experimental groups: G1 and G2, which were control groups; G3, G4, and G5, which were administered [2 doses of glutamine (orally)] separated by a 24-h period (150, 300, and 600 mg/kg, respectively), and a dose of phosphate-buffered saline by intraperitoneal injection; G6, G7, and G8, which were treated in the same manner as the previous groups, but received cisplatin rather than phosphate-buffered saline. The antimutagenicity groups showed damage reduction percentages of 79.05, 80.00, and 94.27% at the time point T1, 53.18, 67.05, and 64.74 at time point T2 for the 150, 300, and 600 mg/kg doses of glutamine, respectively. Antigenotoxic activity was evident for all 3 doses with damage reduction percentages of 115.05, 119.06, and 114.38 for the doses of glutamine of 150, 300, and 600 mg/ kg, respectively. These results suggest that further studies are needed to confirm the clastogenic activity of glutamine. However, our results may lead to rational strategies for supplementation of this antioxidant as an adjuvant in cancer treatment or for preventing genomic lesions.
authors: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors: J R
authors: JR
authors: Pesarini
authors: School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, United Kingdom.
authors: S G
authors: SG
authors: Victorelli
authors: Centro de Estudos em Nutrição e Genética Toxicológica, Centro Universitário Filadélfia, Londrina, PR, Brasil.
authors: A P
authors: AP
authors: Vicentini
authors: Centro de Estudos em Nutrição e Genética Toxicológica, Centro Universitário Filadélfia, Londrina, PR, Brasil.
authors: L K
authors: LK
authors: Ferreira
authors: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors: M O
authors: MO
authors: Mauro
authors: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors: R
authors: R
authors: Matuo
authors: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors: J R
authors: JR
authors: Oliveira
authors: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors: A C M B
authors: AC
authors: Antoniolli
authors: Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors: M S
authors: MS
authors: Mantovani
authors: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil rodrigo.oliveira@ufms.br.
authors: R J
authors: RJ
authors: Oliveira
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D002945:Cisplatin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage; D005973:Glutamine / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000494:pharmacology*
chemical_list: D016587:Antimutagenic Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D009153:Mutagens; D005973:Glutamine; D002945:Cisplatin