title: Studying the mechanism of CD47-SIRPα interactions on red blood cells by single molecule force spectroscopy.
abstract: The interaction forces and binding kinetics between SIRPα and CD47 were investigated by single-molecule force spectroscopy (SMFS) on both fresh and experimentally aged human red blood cells (hRBCs). We found that CD47 experienced a conformation change after oxidation, which influenced the interaction force and the position of the energy barrier between SIRPα and CD47. Our results are significant for understanding the mechanism of phagocytosis of red blood cells at the single molecule level.
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China. hdwang@ciac.ac.cn.
authors: Yangang
authors: Y
authors: Pan
authors: Feng
authors: F
authors: Wang
authors: Yanhou
authors: Y
authors: Liu
authors: Junguang
authors: J
authors: Jiang
authors: Yong-Guang
authors: YG
authors: Yang
authors: Hongda
authors: H
authors: Wang
mesh_terms: D000943:Antigens, Differentiation / Q000737:chemistry* / Q000648:ultrastructure; D001665:Binding Sites; D051928:CD47 Antigen / Q000737:chemistry* / Q000648:ultrastructure; D002478:Cells, Cultured; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D007700:Kinetics; D018625:Microscopy, Atomic Force / Q000379:methods*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000648:ultrastructure; D013314:Stress, Mechanical
chemical_list: D000943:Antigens, Differentiation; D051928:CD47 Antigen; C497894:CD47 protein, human; D011971:Receptors, Immunologic; C501920:SIRPA protein, human