title: Manno-oligosaccharide-binding ability of mouse RegIV/GST-fusion protein evaluated by complex formation with the carbohydrate-containing polyamidoamine dendrimer.
abstract: The carbohydrate-binding properties of the C-type lectin-like mouse RegIV and glutathione S-transferase-fusion protein (GST-mRegIV) were examined using carbohydrate-containing polyamidoamine dendrimers (PD). GST-mRegIV showed affinity for mannan- and manno-oligosaccharide containing PD. Binding was inhibited by manno-oligosaccharides but not by mannose or other tested carbohydrates, suggesting that the binding site may have an extended structure in contrast with typical C-type lectins.
authors: a Biomolecular Chemistry Laboratory, Graduate School of Engineering , Nagasaki University , Nagasaki , Japan.
authors: Yuta
authors: Y
authors: Kato
authors: Kazunori
authors: K
authors: Kochi
authors: Hideaki
authors: H
authors: Unno
authors: Shuichiro
authors: S
authors: Goda
authors: Tomomitsu
authors: T
authors: Hatakeyama
mesh_terms: D000818:Animals; D056831:Coordination Complexes / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry / Q000378:metabolism*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008358:Mannose / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008958:Models, Molecular; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D011073:Polyamines / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D050091:Dendrimers; D037102:Lectins; D037181:Lectins, C-Type; D009844:Oligosaccharides; D000073718:Pancreatitis-Associated Proteins; C531249:Poly(amidoamine); D011073:Polyamines; C428528:REG4 protein, human; D005982:Glutathione Transferase; D008358:Mannose
keywords: RegIV; binding; light scattering; mannose; polyamidoamine dendrimer