title: Genome-wide survey of Alternative Splicing in Sorghum Bicolor.
abstract: Sorghum bicolor is a member of grass family which is an attractive model plant for genome study due to interesting genome features like low genome size. In this research, we performed comprehensive investigation of Alternative Splicing and ontology aspects of genes those have undergone these events in sorghum bicolor. We used homology based alignments between gene rich transcripts, represented by tentative consensus (TC) transcript sequences, and genomic scaffolds to deduce the structure of genes and identify alternatively spliced transcripts in sorghum. Using homology mapping of assembled expressed sequence tags with genomics data, we identified 2,137 Alternative Splicing events in S. bicolor. Our study showed that complex events and intron retention are the main types of Alternative Splicing events in S. bicolor and highlights the prevalence of splicing site recognition for definition of introns in this plant. Annotations of the alternatively spliced genes revealed that they represent diverse biological process and molecular functions, suggesting a fundamental role for Alternative Splicing in affecting the development and physiology of S. bicolor.
authors: Department of Biotechnology and Plant Breeding, University of Tabriz, Tabriz, Iran.
authors: Bahman
authors: B
authors: Panahi
authors: Meshgin shahr Branch, Islamic Azad University, Meshgin Shahr, Iran.
authors: Bahram
authors: B
authors: Abbaszadeh
authors: Department of Biotechnology and Plant Breeding, University of Tabriz, Tabriz, Iran.
authors: Mehdi
authors: M
authors: Taghizadeghan
authors: School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, Australia.
authors: Esmaeil
authors: E
authors: Ebrahimie
keywords: Alternative splicing; Complex events; Intron retention; Ontology