title: Paeoniflorin inhibited the tumor invasion and metastasis in human hepatocellular carcinoma cells.
abstract: OBJECTIVE
  Evidence suggests that paeoniflorin may be involved in anticancer activities. Here, we have investigated the effects of paeoniflorin and correlative mechanisms on anti-invasion and anti-metastasis in human hepatocellular carcinoma (HCC) cell lines.
  
  
  METHODS
  In the current study, we have applied 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay to determine the proliferative effect of HepG2 and Bel-7402, two human hepatoma cell lines, and we have established a boyden chamber assay, a wound healing assay and cell adhesion assay to detect and quantify the invasion, metastasis and adhesion of both HepG2 and Bel-7402. In addition, we have analyzed the protein expression of matrix metalloproteinas (MMP)-9, E-cadherin (E-cad) and extracellular signal-regulated kinase (ERK) in both cell lines through western blot analysis.
  
  
  RESULTS
  Paeoniflorin (6. 25-200 µM) had inhibitory effect on the growth of HepG2 and Bel-7402 cell lines, and reduced significantly invasion, metastasis and adhesion of HCC cell lines. In addition, paeoniflorin decreased the expression of MMP-9 and ERK in HepG2 and Bel-7402 cells, and increased expression of E-cad in both cell lines.
  
  
  CONCLUSIONS
  Paeoniflorin is effective anti-metastatic and anti-invasive agent for suppressing HCC invasion and metastasis (Fig. 5, Ref. 30).
authors: J T
authors: JT
authors: Lu
authors: W
authors: W
authors: He
authors: S S
authors: SS
authors: Song
authors: W
authors: W
authors: Wei
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use*; D015820:Cadherins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology* / Q000556:secondary; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005960:Glucosides / Q000627:therapeutic use*; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008558:Melphalan / Q000494:pharmacology; D039821:Monoterpenes / Q000627:therapeutic use*; D009361:Neoplasm Invasiveness / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control; D029596:Paeonia; D010770:Phosphotransferases / Q000378:metabolism; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D006027:Glycosides
chemical_list: D000970:Antineoplastic Agents; D015820:Cadherins; D005960:Glucosides; D007074:Immunoglobulin G; D020780:Matrix Metalloproteinase 9; D008558:Melphalan; D039821:Monoterpenes; D010770:Phosphotransferases; D028321:Plant Preparations; C051713:antineoplastic agent K 18; C015423:peoniflorin; D006027:Glycosides