title: Function of chalcone reductase gene CHR1 in soybean.
abstract: To verify the function of chalcone reductase gene (CHR1) in soybean Daidzein synthesis, CHR1 gene in soybean was cloned, and an RNAi expression vector pCPB-CHR1-RNAi was constructed. Four transformed plants in T0 generation and thirteen transformed plants in T1 generation of soybean "Jinong28" were obtained by agrobacterium-mediated genetic transformation, in which transcriptions of CHR1 gene were depressed. Southern blotting showed the functional fragment of pCPB-CHR1-RNAi was integrated into the genome of recipient soybean in the form of a single copy. Detection of the transcription of CHR1 gene using quantitative real-time PCR (qRT-PCR) showed that the expression of CHR1 gene in transformed plants decreased 60%-99% compared to the recipient soybean, while the content of isoliquiritigenin, the precursors of daidzein, decreased 38.7%. These results indicate that RNA interference can suppress the transcription of CHR1 gene expression successfully.
authors: College of Agriculture, Jilin Agricultural University, Changchun 130118, China.
authors: Nan
authors: N
authors: Wu
authors: College of Agriculture, Jilin Agricultural University, Changchun 130118, China.
authors: Piwu
authors: P
authors: Wang
authors: College of Agriculture, Jilin Agricultural University, Changchun 130118, China.
authors: Dan
authors: D
authors: Li
authors: College of Agriculture, Jilin Agricultural University, Changchun 130118, China.
authors: Liqiang
authors: L
authors: Dai
authors: College of Agriculture, Jilin Agricultural University, Changchun 130118, China.
authors: Chengzhong
authors: C
authors: Zheng
authors: College of Agriculture, Jilin Agricultural University, Changchun 130118, China.
authors: Shi
authors: S
authors: Lu
authors: College of Agriculture, Jilin Agricultural University, Changchun 130118, China.
authors: Yuan
authors: Y
authors: Cai
authors: College of Agriculture, Jilin Agricultural University, Changchun 130118, China.
authors: Zhuo
authors: Z
authors: Zhang
authors: College of Agriculture, Jilin Agricultural University, Changchun 130118, China.
authors: Jing
authors: J
authors: Qu
authors: 1. College of Agriculture, Jilin Agricultural University, Changchun 130118, China; 2. Tonghua Academy of Agricultural Sciences, Meihekou, Jilin 135000, China;
authors: Haifeng
authors: H
authors: Xia
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D007529:Isoflavones / Q000096:biosynthesis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D007529:Isoflavones; D010940:Plant Proteins; C004742:daidzein; D000429:Alcohol Oxidoreductases; C093070:chalcone reductase