title: Structure and gene cluster of the O antigen of Escherichia coli L-19, a candidate for a new O-serogroup.
abstract: Escherichia coli L-19 isolated from a healthy individual did not agglutinate with any of 21 polyvalent antisera that cover 174 E. coli O-serogroups. The strain was studied in respect to the O-antigen (O-specific polysaccharide, OPS) structure and genetics. The LPS was isolated by phenol-water extraction of bacterial cells and cleaved by mild acid hydrolysis to yield the OPS. The OPS was studied by sugar and methylation analyses, along with 1D and 2D (1)H and (13)C NMR spectroscopy. The established structure of the linear tetrasaccharide repeating unit was found to be unique among known bacterial polysaccharide structures. A peculiar component of the L-19 OPS was an amide of glucuronic acid with 2-amino-1,3-propanediol (2-amino-2-deoxyglycerol) (GroN). The O-antigen gene cluster of L-19 between the conserved genes galF and gnd was sequenced, and gene functions were tentatively assigned by a comparison with sequences in the available databases and found to be in agreement with the OPS structure. Except for putative genes for synthesis and transfer of GroN, the sequences in the L-19 O-antigen gene cluster were little related to those of reference strains of the 174 known E. coli O-serogroups. The data obtained suggest that L-19 can be considered as a candidate for a new E. coli O-serogroup.
authors: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospekt 47, 119991 Moscow, Russia.
authors: Evelina L
authors: EL
authors: Zdorovenko
authors: D. K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Akademik Zabolotny Street 154, 03680 Kyiv, GSP, Ukraine.
authors: Lyudmila D
authors: LD
authors: Varbanets
authors: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, 300457 Tianjin, PR China.
authors: Bin
authors: B
authors: Liu
authors: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospekt 47, 119991 Moscow, Russia.
authors: Olga A
authors: OA
authors: Valueva
authors: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, 300457 Tianjin, PR China.
authors: Quan
authors: Q
authors: Wang
authors: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospekt 47, 119991 Moscow, Russia.
authors: Alexander S
authors: AS
authors: Shashkov
authors: National Aviation University, Kosmonavt Komarov Prospekt 1, 03058 Kyiv, GSP, Ukraine.
authors: Ekaterina G
authors: EG
authors: Garkavaya
authors: D. K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Akademik Zabolotny Street 154, 03680 Kyiv, GSP, Ukraine.
authors: Oksana S
authors: OS
authors: Brovarskaya
authors: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, 300457 Tianjin, PR China.
authors: Lei
authors: L
authors: Wang
authors: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospekt 47, 119991 Moscow, Russia.
authors: Yuriy A
authors: YA
authors: Knirel
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D065288:Serogroup
chemical_list: D004269:DNA, Bacterial; D019081:O Antigens