title: Identification and characterization of ace2-type acetylcholinesterase in insecticide-resistant and -susceptible parasitoid wasp Oomyzus sokolowskii (Hymenoptera: Eulophidae).
abstract: A full-length acetylcholinesterase (AChE) cDNA sequence (Os-ace2.s) from insecticide-susceptible (S) parasitoid Oomyzus sokolowskii (Hymenoptera: Eulophidae) and a partial cDNA sequence (Os-ace2.r) from insecticide- resistant (R) O. sokolowskii were identified firstly. Both Os-ace2.s (encoding a protein of 639 amino acid residues) and Os-ace2.r (encoding a protein of 530 amino acid residues) contained the typical conserved motifs, including FGESAGdomains, catalytic triad, acyl pocket, three oxy-anino hole, choline binding site, peripheral anionic site, omega loop and conserved aromatic residues. The multiple alignment and Blast results indicated that Os-ace2.s were ace2 member of AChE gene. There were three replacements of the amino acid residues (Glu 115 Leu, Phe 394 Leu, and Lys 424 Arg) between Os-ace2.s and Os-ace2.r. The ace2 of O. sokolowskii was the AChE gene firstly isolated from hymenopteran parasitoid so far. R O. sokolowskii displayed about 15-20-folds resistance ratios to methamidophos and avermectin. The bimolecular rate constant (k i) value in S O. sokolowskii was 3.8-folds for methamidophos and 12.3 for dichlorvos, respectively higher than those in R O. sokolowskii. The results indicated that the insensitive AChE and replacements of the amino acid residues in Os-ace2 might be involved in the resistance to methamidophos in R O. sokolowskii.
authors: Key Laboratory of Biopesticide and Chemical Biology (Fujian Agriculture and Forestry University), Ministry of Education, Fuzhou, 350002, Fujian, China.
authors: Hua Mei
authors: HM
authors: Zhuang
authors: Chang Wei
authors: CW
authors: Li
authors: Gang
authors: G
authors: Wu
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D019295:Computational Biology; D017124:Conserved Sequence / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000096:biosynthesis; D007305:Insecticide Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D009946:Organothiophosphorus Compounds; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014863:Wasps / Q000201:enzymology* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D009946:Organothiophosphorus Compounds; C014655:methamidophos; D000110:Acetylcholinesterase