title: Nucleotide and amino acid variations of tannase gene from different Aspergillus strains.
abstract: Tannase is an enzyme that catalyses the hydrolysis of ester bonds present in tannins. Most of the scientific reports about this biocatalysis focus on aspects related to tannase production and its recovery; on the other hand, reports assessing the molecular aspects of the tannase gene or protein are scarce. In the present study, a tannase gene fragment from several Aspergillus strains isolated from the Mexican semidesert was sequenced and compared with tannase amino acid sequences reported in NCBI database using bioinformatics tools. The genetic relationship among the different tannase sequences was also determined. A conserved region of 7 amino acids was found with the conserved motif GXSXG common to esterases, in which the active-site serine residue is located. In addition, in Aspergillus niger strains GH1 and PSH, we found an extra codon in the tannase sequences encoding glycine. The tannase gene belonging to semidesert fungal strains followed a neutral evolution path with the formation of 10 haplotypes, of which A. niger GH1 and PSH haplotypes are the oldest.
authors: Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Blvd. Venustiano Carranza and José Cárdenas s/n, Republica Oriente, ZIP 25280, Saltillo, Coahuila, México.
authors: J A
authors: JA
authors: Borrego-Terrazas
authors: F
authors: F
authors: Lara-Victoriano
authors: A C
authors: AC
authors: Flores-Gallegos
authors: F
authors: F
authors: Veana
authors: C N
authors: CN
authors: Aguilar
authors: R
authors: R
authors: Rodríguez-Herrera
mesh_terms: D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology*; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics*; D017124:Conserved Sequence; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005800:Genes, Fungal; D006239:Haplotypes; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D005656:Fungal Proteins; D002265:Carboxylic Ester Hydrolases; C022711:tannase
keywords: conserved motif; liens phylogénétiques; motif conservé; phylogenetic relationships; sequencing; séquençage; tannin acylhydrolase