title: Comparison of the biological properties of ProRoot MTA, OrthoMTA, and Endocem MTA cements.
abstract: BACKGROUND
  OrthoMTA (BioMTA, Seoul, Korea) and Endocem MTA (Maruchi, Wonju-si, Korea) were recently developed to overcome the disadvantages of ProRoot MTA (Dentsply, Tulsa, OK). This study aimed to compare the biological properties of OrthoMTA and Endocem MTA with those of ProRoot MTA using the preosteoblastlike cell line MC3T3-E1.
  
  
  METHODS
  The setting times of calcium silicate-based cements (CSCs) and their effects on the pH of distilled water during storage were determined according to ISO standards. MC3T3-E1 cells were cultured with ProRoot MTA, OrthoMTA, and Endocem MTA. The viability of the cells was assessed using the Cell Counting Kit-8 assay (Dojindo Laboratory, Kumamoto, Japan) on the supernatants of CSCs, and the cells' osteopontin production was determined by an enzyme-linked immunosorbent assay on a culture with the materials on days 3 and 7 of incubation.
  
  
  RESULTS
  Endocem MTA exhibited a significantly shorter setting time (15.3 ± 0.5 minutes) than did ProRoot MTA and OrthoMTA (318.0 ± 56.0 and 324.3 ± 2.1 minutes, P < .05). Additionally, all CSCs caused their storage water to become highly alkaline after 7 days. OrthoMTA was significantly more cytotoxic than ProRoot and Endocem MTA (P < .05). ProRoot MTA induced significantly more OPN production than OrthoMTA and Endocem MTA on both days 3 and 7 (P < .05).
  
  
  CONCLUSIONS
  ProRoot MTA appeared to be superior to OrthoMTA and Endocem MTA in terms of biological properties although Endocem MTA exhibited the shortest setting time and presented lower cytotoxicity with osteoblastlike cells.
authors: Department of Conservative Dentistry, Ulsan University, Asan Medical Center, Seoul, Korea.
authors: Miri
authors: M
authors: Kim
authors: Department of Conservative Dentistry, Ulsan University, Asan Medical Center, Seoul, Korea.
authors: Wonkyung
authors: W
authors: Yang
authors: Department of Conservative Dentistry, Seoul National University Boramae Hospital, Seoul, Korea.
authors: Heesun
authors: H
authors: Kim
authors: Department of Conservative Dentistry, Ulsan University, Asan Medical Center, Seoul, Korea. Electronic address: hyunjko@gmail.com.
authors: Hyunjung
authors: H
authors: Ko
mesh_terms: D016475:3T3 Cells; D017607:Aluminum Compounds / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D004338:Drug Combinations; D006244:Hardness; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects; D053495:Osteopontin / Q000032:analysis / Q000187:drug effects; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; C525641:MTA Bio; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C586519:endocem; C086631:mineral trioxide aggregate; D014867:Water; D053495:Osteopontin; C031293:calcium silicate
keywords: Calcium silicate–based cements; Endocem MTA; MC3T3-E1 cell; OrthoMTA; ProRoot MTA; cytotoxicity; osteopontin