title: Effect of standard and neutral-pH peritoneal dialysis solutions upon fibroblasts proliferation.
abstract: BACKGROUND
  Continuous exposition of the peritoneal membrane to conventional dialysis solutions is an important risk factor for inducing structural and functional alterations.
  
  
  OBJECTIVE
  To compare in vitro mouse fibroblast NIH-3T3 cell viability after exposition to a neutral pH dialysis solution in comparison to cells exposed to a standard solution.
  
  
  METHODS
  Experimental study to compare the effects of a conventional standard or a neutral-pH, low-glucose degradation products peritoneal dialysis solution on the viability of exposed fibroblasts in cell culture. Both solutions were tested in all the commercially available glucose concentrations. Cell viability was evaluated with tetrazolium salt colorimetric assay.
  
  
  RESULTS
  Fibroblast viability was significantly superior in the neutral pH solution in comparison to control, in all three glucose concentrations (Optical density in nm-means ± SD: 1.5% 0.295 ± 0.047 vs. 0.372 ± 0.042, p < 0.001; 2.3% 0.270 ± 0.036 vs. 0.337 ± 0.051, p < 0.001; 4.25% 0.284 ± 0.037 vs. 0.332 ± 0.032, p < 0.001; control vs. neutral pH respectively, Student t Test). There was no significant difference in cell viability between the three concentrations of glucose when standard solution was used (ANOVA p = 0.218), although cell viability was higher after exposition to neutral pH peritoneal dialysis fluid at 1.5% in comparison to 2.3 and 4.25% glucose concentrations (ANOVA p = 0.008: Bonferroni 1.5% vs. 2.3% p = 0.033, 1.5% vs. 4.25% p = 0.014, 2.3% vs. 4.25% p = 1.00).
  
  
  CONCLUSIONS
  Cell viability was better in neutral pH dialysis solution, especially in the lower glucose concentration. A more physiological pH and lower glucose degradation products may be responsible for such results.
authors: Pontifical Catholic University of Rio Grande do Sul.
authors: André Antunes
authors: AA
authors: Poitevin
authors: Pontifical Catholic University of Rio Grande do Sul.
authors: Christian
authors: C
authors: Viezzer
authors: Pontifical Catholic University of Rio Grande do Sul.
authors: Denise Cantarelli
authors: DC
authors: Machado
authors: Pontifical Catholic University of Rio Grande do Sul.
authors: Bartira Ercilia Pinheiro
authors: BE
authors: da Costa
authors: Pontifical Catholic University of Rio Grande do Sul.
authors: Ana Elizabeth
authors: AE
authors: Figueiredo
authors: Pontifical Catholic University of Rio Grande do Sul.
authors: Domingos
authors: D
authors: d'Avila
authors: Pontifical Catholic University of Rio Grande do Sul.
authors: Carlos Eduardo
authors: CE
authors: Poli-de-Figueiredo
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D015314:Dialysis Solutions / Q000737:chemistry* / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D010530:Peritoneal Dialysis
chemical_list: D015314:Dialysis Solutions