title: Agent selection and protective effects during single droplet drying of bacteria.
abstract: The protective mechanisms of whey protein isolate (WPI), trehalose, lactose, and skim milk on Lactobacillus plantarum A17 during convective droplet drying has been explored. A single droplet drying technique was used to monitor cell survival, droplet temperature and corresponding changes in mass. WPI and skim milk provided the highest protection amongst the materials tested. In situ analysis of the intermediate stage of drying revealed that for WPI and skim milk, crust formation reduces the rate of sudden temperature increase thereby imparting less stress on the cells. Irreversible denaturation of the WPI components might have also contributed to the protection of the cells. Skim milk, however, 'loses' the protective behaviour towards the latter stages of drying. This indicates that the concentration of the WPI components could be another possible factor determining the sustained protective behaviour during the later stages of drying when the moisture content is low.
authors: School of Applied Sciences, RMIT University, 124 La Trobe St, Melbourne, Victoria 3001, Australia.
authors: Sarim
authors: S
authors: Khem
authors: Department of Chemical Engineering, Monash University, Clayton, Victoria 3800, Australia.
authors: Meng Wai
authors: MW
authors: Woo
authors: School of Applied Sciences, RMIT University, 124 La Trobe St, Melbourne, Victoria 3001, Australia.
authors: Darryl M
authors: DM
authors: Small
authors: Department of Chemical Engineering, Monash University, Clayton, Victoria 3800, Australia; Chemical Engineering Innovation Laboratory, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Suzhou, Jiangsu Province, China.
authors: Xiao Dong
authors: XD
authors: Chen
authors: School of Applied Sciences, RMIT University, 124 La Trobe St, Melbourne, Victoria 3001, Australia. Electronic address: bee.may@rmit.edu.au.
authors: Bee K
authors: BK
authors: May
mesh_terms: D048191:Lactiplantibacillus plantarum / Q000737:chemistry*; D007785:Lactose / Q000737:chemistry*; D014199:Trehalose / Q000737:chemistry*
chemical_list: D014199:Trehalose; D007785:Lactose
keywords: Lactobacillus plantarum; Lactose; Protective mechanism; Single droplet drying; Trehalose; Whey protein isolate