title: Timing and recovery of postweaning exposure to diethylstilbestrol on early pregnancy in CD-1 mice.
abstract: Exposure timing could play an important role in the effects of estrogenic endocrine disrupting chemicals (EEDCs) on early pregnancy. This study examined the sensitivity of different exposure periods from weaning to gestation day 4.5 (D4.5) to 50ppb diethylstilbestrol (DES, a test EEDC) diet on embryo implantation and potential recovery upon temporary cessation of DES exposure in CD-1 mice. Peripubertal (3-5 weeks old) DES exposure reduced the numbers of corpora lutea and implantation sites. Postpubertal (5-7 weeks old) DES exposure did not have significant effects on early pregnancy. Postmating (D0.5-D4.5) DES exposure affected postovulation events leading to impaired embryo implantation. A 5-day premating rest from 5-week DES exposure (3-8 weeks old) resulted in recovery of early pregnancy rate. These data demonstrate that peripubertal and postmating periods are sensitive windows to endocrine disruption of early pregnancy and temporary cessation of exposure could partially alleviate adverse effects of DES on early pregnancy.
authors: Department of Physiology & Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA; Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602, USA. Electronic address: feizhao@uga.edu.
authors: Fei
authors: F
authors: Zhao
authors: Department of Physiology & Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA; Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602, USA. Electronic address: junzhou9@uga.edu.
authors: Jun
authors: J
authors: Zhou
authors: Department of Physiology & Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA; Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602, USA. Electronic address: ahmdezat@uga.edu.
authors: Ahmed E
authors: AE
authors: El Zowalaty
authors: Department of Physiology & Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA; Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602, USA. Electronic address: lirong9@uga.edu.
authors: Rong
authors: R
authors: Li
authors: Department of Physiology & Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA; Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602, USA. Electronic address: ldudley9@uga.edu.
authors: Elizabeth A
authors: EA
authors: Dudley
authors: Department of Physiology & Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA; Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602, USA. Electronic address: ye@uga.edu.
authors: Xiaoqin
authors: X
authors: Ye
mesh_terms: D000818:Animals; D004054:Diethylstilbestrol / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D010064:Embryo Implantation / Q000187:drug effects; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D008297:Male; D051379:Mice; D010053:Ovary / Q000187:drug effects; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D012741:Sexual Maturation / Q000187:drug effects; D013997:Time Factors
chemical_list: D004968:Estrogens, Non-Steroidal; D004054:Diethylstilbestrol
keywords: Corpus luteum; Diethylstilbestrol (DES); Early pregnancy; Embryo implantation; Embryo transport; Estrogenic endocrine disrupting chemical (EEDC); Peripubertal exposure; Postmating exposure