title: Incorporation of gold-coated microspheres into embryoid body of human embryonic stem cells for cardiomyogenic differentiation.
abstract: Human embryonic stem cells (hESCs) are a useful cell source for cardiac regeneration by stem cell therapy. In this study, we show that incorporation of gold-coated microspheres into hESC-derived embryoid bodies (EBs) enhances the cardiomyogenic differentiation process of pluripotent embryonic stem cells. A polycaprolactone (PCL) microsphere surface was coated with gold. Either gold-coated PCL microspheres (AuMS) or PCL microspheres (MS) were incorporated into hESC-derived EBs. AuMS and MS were not cytotoxic. AuMS promoted the expression of genes for mesodermal and cardiac mesodermal lineage cells, both of which are intermediates in the process of cardiac differentiation of hESCs on day 4 and the expression of cardiomyogenic differentiation markers on day 14 compared to MS. AuMS also enhanced gene expression of cardiac-specific extracellular matrices. Incorporation of gold-coated MS into hESC-derived EBs may provide a new platform for inducing cardiomyogenic differentiation of pluripotent embryonic stem cells.
authors: 1 Engineering Research Institute, Seoul National University , Seoul, Republic of Korea.
authors: Tae-Jin
authors: TJ
authors: Lee
authors: Seokyung
authors: S
authors: Kang
authors: Gun-Jae
authors: GJ
authors: Jeong
authors: Jeong-Kee
authors: JK
authors: Yoon
authors: Suk Ho
authors: SH
authors: Bhang
authors: Jaesur
authors: J
authors: Oh
authors: Byung-Soo
authors: BS
authors: Kim
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D019070:Cell Lineage / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D058732:Embryoid Bodies / Q000166:cytology* / Q000187:drug effects; D004707:Endoderm / Q000166:cytology; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006046:Gold / Q000494:pharmacology*; D006801:Humans; D008648:Mesoderm / Q000166:cytology; D008863:Microspheres; D008954:Models, Biological; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009928:Organ Specificity / Q000187:drug effects; D011091:Polyesters / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D020099:Coated Materials, Biocompatible; D011091:Polyesters; D012333:RNA, Messenger; C016240:polycaprolactone; D006046:Gold