title: A human pluripotent stem cell surface N-glycoproteome resource reveals markers, extracellular epitopes, and drug targets.
abstract: Detailed knowledge of cell-surface proteins for isolating well-defined populations of human pluripotent stem cells (hPSCs) would significantly enhance their characterization and translational potential. Through a chemoproteomic approach, we developed a cell-surface proteome inventory containing 496 N-linked glycoproteins on human embryonic (hESCs) and induced PSCs (hiPSCs). Against a backdrop of human fibroblasts and 50 other cell types, >100 surface proteins of interest for hPSCs were revealed. The >30 positive and negative markers verified here by orthogonal approaches provide experimental justification for the rational selection of pluripotency and lineage markers, epitopes for cell isolation, and reagents for the characterization of putative hiPSC lines. Comparative differences between the chemoproteomic-defined surfaceome and the transcriptome-predicted surfaceome directly led to the discovery that STF-31, a reported GLUT-1 inhibitor, is toxic to hPSCs and efficient for selective elimination of hPSCs from mixed cultures.
authors: Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, Hong Kong, SAR ; National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA ; Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Kenneth R
authors: KR
authors: Boheler
authors: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors: Subarna
authors: S
authors: Bhattacharya
authors: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors: Erin M
authors: EM
authors: Kropp
authors: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors: Sandra
authors: S
authors: Chuppa
authors: National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Daniel R
authors: DR
authors: Riordon
authors: Department of Biology, Institute of Molecular Systems Biology, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 16, 8093 Zurich, Switzerland.
authors: Damaris
authors: D
authors: Bausch-Fluck
authors: Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors: Paul W
authors: PW
authors: Burridge
authors: Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors: Joseph C
authors: JC
authors: Wu
authors: National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Robert P
authors: RP
authors: Wersto
authors: Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, Hong Kong, SAR ; Department of Pediatrics & Adolescent Medicine, Hong Kong University, Hong Kong, SAR.
authors: Godfrey Chi Fung
authors: GC
authors: Chan
authors: Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA ; Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA ; Blood Research Institute, Blood Center of Wisconsin, Milwaukee, WI 53226, USA.
authors: Sridhar
authors: S
authors: Rao
authors: Department of Biology, Institute of Molecular Systems Biology, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 16, 8093 Zurich, Switzerland.
authors: Bernd
authors: B
authors: Wollscheid
authors: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors: Rebekah L
authors: RL
authors: Gundry
mesh_terms: D002478:Cells, Cultured; D000939:Epitopes / Q000032:analysis* / Q000276:immunology*; D005434:Flow Cytometry; D006023:Glycoproteins / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D039904:Pluripotent Stem Cells / Q000276:immunology* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000939:Epitopes; D006023:Glycoproteins; D020543:Proteome