title: Cell tri-culture for cardiac vascularization.
abstract: Poor graft survival is a critical obstacle toward production of clinically relevant engineered tissues. Here we utilize a multicellular culturing approach for induction of vascular networks embedded within cardiac tissue constructs. The construct is composed of human cardiomyocytes, endothelial cells (ECs), and embryonic fibroblast cells co-seeded onto highly porous three-dimensional (3D) scaffolds. The resulting vascularized cardiac constructs showed microstructural details characteristic of cardiomyocytes and nascent vessels and exhibited synchronous beating activity in vitro. Upon implantation, stable grafts were formed presenting intense vascularization, with evidence of anastomosis between the pre-formed endothelial capillaries and host neovessels.
authors: Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
authors: Ayelet
authors: A
authors: Lesman
authors: Lior
authors: L
authors: Gepstein
authors: Shulamit
authors: S
authors: Levenberg
mesh_terms: D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid