title: Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats.
abstract: OBJECTIVE
  To evaluate the effects of acupuncture (Ac) and electroacupuncture (EAc) on oxidative stress and inflammation in testis torsion/detorsion (T/D) model in rats.
  
  
  METHODS
  Thirty male Wistar rats were randomized into five groups. G1 Group (Sham) served as control. The remaining groups were submitted to spermatic cord torsion (720°) for 3 hours, followed by detorsion and reperfusion for 4 hours. Before detorsion G3, G4 and G5 rats were treated with Ac, EAc 2Hz and EAc 10 Hz, respectively, applied to acupoint Gulai (S-29) bilaterally under anesthesia for 5 minutes. Next, the testes were detorsioned and reperfused for 4 hours. Afterwards, blood samples and the right testis were collected for biochemical assays: reduced Glutathione (GSH), Malonaldehyde (MDA), Myeloperoxidase (MPO).
  
  
  RESULTS
  EAc stimulation (2 and 10 Hz) promoted significant increase in concentrations of GSH in plasma and testis of G4-G5 rats, compared with G1. There was significant increase of tissue MDA in groups G4-G5 and plasma MDA in all groups, compared with G1. There was a significant reduction in MPO activity in groups G4-G5 compared with G1.
  
  
  CONCLUSIONS
  Electroacupuncture stimulation (2 and 10 Hz) attenuates oxidative stress and inflammatory response in rats subjected to testicular torsion/detorsion.
authors: Department of Surgery, Federal University of Ceara, Brazil.
authors: Porfirio Cezar Passos
authors: PC
authors: Acioli
authors: Nursing School, UFC, Fortaleza, CE, Brazil.
authors: Andrea de Oliveira
authors: Ade O
authors: Albuquerque
authors: Department of Surgery, UFC, Fortaleza, CE, Brazil.
authors: Isaac Bevilaqua de Albuquerque
authors: IB
authors: Guimarães
authors: Department of Pathology, UFC, Fortaleza, CE, Brazil.
authors: Roberto Wagner Bezerra de
authors: RW
authors: Araujo
authors: Department of Surgery, UFC, Fortaleza, CE, Brazil.
authors: Paulo Roberto Leitão
authors: PR
authors: Vasconcelos
authors: UFC, Fortaleza, CE, Brazil.
authors: Sérgio Botelho
authors: SB
authors: Guimarães
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D015671:Electroacupuncture / Q000379:methods*; D005978:Glutathione / Q000097:blood; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress; D009195:Peroxidase / Q000097:blood; D011897:Random Allocation; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000628:therapy; D015203:Reproducibility of Results; D013086:Spermatic Cord Torsion / Q000378:metabolism / Q000628:therapy*; D013737:Testis / Q000098:blood supply / Q000378:metabolism; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D008315:Malondialdehyde; D009195:Peroxidase; D005978:Glutathione