title: Expression of FK506-binding protein 52 (FKBP52) in chorionic villi with early recurrent spontaneous abortion.
abstract: OBJECTIVE
  To investigate the mRNA and protein expression of FK506-binding protein 52 (FKBP52) in the chorionic villi of patients with recurrent spontaneous abortion (RSA) and normal women during early pregnancy.
  
  
  METHODS
  Fresh chorionic villus tissues were collected from 60 subjects. A total of 30 patients with a history of RSA were enrolled into the RSA group and 30 normal pregnant women were enrolled into the control group. The FKBP52 mRNA expression levels in chorionic villi of the RSA patients and healthy controls were measured via semiquantitative RT-PCR. The protein distribution and expression levels of FKBP52 in chorionic villi were analyzed through immunohistochemistry (IHC). The correlation between FKBP52 expression and RSA was analyzed.
  
  
  RESULTS
  We demonstrated that FKBP52 mRNA is expressed in chorionic villi samples of normal pregnancy and RSA. RSA patients exhibited significantly lower FKBP52 gene expression levels compared with those in normal pregnancies (p < 0.05). FKBP52 immunoreactivity in chorionic villi was mainly observed in trophoblast cell cytoplasm. The FKBP52 protein expression levels in the chorionic villi of RSA patients was significantly lower than in normal women during pregnancy (p < 0.05).
  
  
  CONCLUSIONS
  FKBP52 protein levels were decreased in the chorionic villi of RSA patients, which indicate that the decrease in FKBP52 may be associated with RSA. The low FKBP52 mRNA expression level, which is consistent with the IHC result, may affect embryonic development and even lead to abortion. FKBP52 may be involved in the pathogenesis of RSA and new therapies that increase the FKBP52 expression may help treat RSA.
authors: a Division of Maternal-Fetal Medicine , Prenatal Diagnosis Center, the First Affiliated Hospital of Guangxi Medical University , Nanning , Guangxi , China and.
authors: Hong-Yan
authors: HY
authors: Chen
authors: Ou-Yang
authors: OY
authors: Li
authors: Li-Hong
authors: LH
authors: Pang
authors: Hong
authors: H
authors: Xu
authors: Xiao-Jing
authors: XJ
authors: Fan
authors: Hai-Feng
authors: HF
authors: Liang
authors: Xian-Feng
authors: XF
authors: Chen
authors: Jun-Zhen
authors: JZ
authors: Qing
authors: Ru-Dian
authors: RD
authors: Huang
authors: Bi-Ye
authors: BY
authors: Deng
mesh_terms: D000026:Abortion, Habitual / Q000378:metabolism*; D000328:Adult; D002824:Chorionic Villi / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D022021:Tacrolimus Binding Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D022021:Tacrolimus Binding Proteins; C403485:tacrolimus binding protein 4
keywords: Chorionic villi; FKBP52; RT-PCR; immunohistochemistry; recurrent spontaneous abortion