title: Analysis of the expression of interleukins, interferon β, and nuclear factor-κ B in prostate cancer and their relationship with biochemical recurrence.
abstract: There are accumulating epidemiological, experimental, and genetic data supporting that prostate inflammation may contribute to prostate carcinogenesis, and several inflammatory-related molecules have been linked to tumorigenesis and prognosis in several tumors. The aim of this study was to evaluate tumor expression of inflammatory-related factors in prostate carcinomas and their possible relationship with biochemical recurrence (elevation of prostate-specific antigen serum levels). An immunohistochemical study was conducted using tissue microarrays and specific antibodies against interleukin-1β (IL-1β), IL-6, IL-10, IL-17, interferon β (IFNβ), and nuclear factor-κ B (NF-κB). Determinations in cancer specimens from 118 patients with primary prostate cancer (78 without and 40 with recurrence during the follow-up period) were performed. Immunostaining for all the studied proteins was localized both in tumor cells and in stromal cells in the majority of tumors. High-score values for IL-1β or low-score values for IFNβ were significantly associated with biochemical recurrence. The analysis defined a score value of 160 for IL-1β and of 170 for IFNβ as the optimal cutoff points that identified 32.7% and 73.2% of patients, respectively, having high probability of biochemical recurrence. Multivariate analysis according to a Cox model indicated that the cutoff point 170 for IFNβ (P=0.035) was an independent factor associated with biochemical recurrence in patients with prostate cancer. Both IL-1β and IFNβ may be new biomarkers to distinguish high-risk/low-risk patients with prostate cancer, and to select appropriate therapeutic approaches.
authors: *Unidad de Investigación †Servicio Urología ‡Servicio de Cirugía General, Fundación Hospital de Jove, Asturias, Gijón, Spain.
authors: Noemí
authors: N
authors: Eiró
authors: Sandra
authors: S
authors: Bermudez-Fernandez
authors: Belen
authors: B
authors: Fernandez-Garcia
authors: Sara
authors: S
authors: Atienza
authors: Nana
authors: N
authors: Beridze
authors: Safwan
authors: S
authors: Escaf
authors: Francisco J
authors: FJ
authors: Vizoso
mesh_terms: D000328:Adult; D000368:Aged; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism*; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D040081:Protein Array Analysis; D012008:Recurrence; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D007378:Interleukins; D016328:NF-kappa B; D016899:Interferon-beta; D017430:Prostate-Specific Antigen