title: Reconstituting ring-rafts in bud-mimicking topography of model membranes.
abstract: During vesicular trafficking and release of enveloped viruses, the budding and fission processes dynamically remodel the donor cell membrane in a protein- or a lipid-mediated manner. In all cases, in addition to the generation or relief of the curvature stress, the buds recruit specific lipids and proteins from the donor membrane through restricted diffusion for the development of a ring-type raft domain of closed topology. Here, by reconstituting the bud topography in a model membrane, we demonstrate the preferential localization of cholesterol- and sphingomyelin-enriched microdomains in the collar band of the bud-neck interfaced with the donor membrane. The geometrical approach to the recapitulation of the dynamic membrane reorganization, resulting from the local radii of curvatures from nanometre-to-micrometre scales, offers important clues for understanding the active roles of the bud topography in the sorting and migration machinery of key signalling proteins involved in membrane budding.
authors: School of Electrical Engineering #032, Seoul National University, Kwanak P.O. Box 34, Seoul 151-600, Republic of Korea.
authors: Yong-Sang
authors: YS
authors: Ryu
authors: School of Electrical Engineering #032, Seoul National University, Kwanak P.O. Box 34, Seoul 151-600, Republic of Korea.
authors: In-Ho
authors: IH
authors: Lee
authors: School of Electrical Engineering #032, Seoul National University, Kwanak P.O. Box 34, Seoul 151-600, Republic of Korea.
authors: Jeng-Hun
authors: JH
authors: Suh
authors: School of Electrical Engineering #032, Seoul National University, Kwanak P.O. Box 34, Seoul 151-600, Republic of Korea.
authors: Seung Chul
authors: SC
authors: Park
authors: World Class University (WCU) Program of Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors: Soojung
authors: S
authors: Oh
authors: Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors: Luke R
authors: LR
authors: Jordan
authors: Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors: Nathan J
authors: NJ
authors: Wittenberg
authors: 1] Department of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA [2] Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors: Sang-Hyun
authors: SH
authors: Oh
authors: World Class University (WCU) Program of Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors: Noo Li
authors: NL
authors: Jeon
authors: School of Electrical Engineering #032, Seoul National University, Kwanak P.O. Box 34, Seoul 151-600, Republic of Korea.
authors: Byoungho
authors: B
authors: Lee
authors: 1] Department of Biomedical Engineering and Chemical Engineering and Materials Science, University of California, Davis, California 95616, USA [2] Centre for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University, Singapore 637553, Singapore.
authors: Atul N
authors: AN
authors: Parikh
authors: School of Electrical Engineering #032, Seoul National University, Kwanak P.O. Box 34, Seoul 151-600, Republic of Korea.
authors: Sin-Doo
authors: SD
authors: Lee
mesh_terms: D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D021621:Imaging, Three-Dimensional; D008051:Lipid Bilayers / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000737:chemistry; D013109:Sphingomyelins / Q000737:chemistry / Q000378:metabolism
chemical_list: D004129:Dimethylpolysiloxanes; D008051:Lipid Bilayers; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D013109:Sphingomyelins; C013830:baysilon; D002784:Cholesterol; C017251:1,2-oleoylphosphatidylcholine