title: Profile of PRMT-1 gene polymorphism in hemodialysis patients with increased ADMA levels.
abstract: OBJECTIVE
  to determine the distribution of PRMT-1 gene polymorphism and ADMA levels among continuing hemodialysis patients.
  
  
  METHODS
  genotyping of PRMT-1 polymorphism was performed in 57 hemodialysis patients at Al Irsjad Hospital. All participants were recruited for physical examination, questionnaire, and collection of 5 mL fasting venous blood. The blood was treated with phenol-chloroform extraction of genomic DNA. The candidate's single nucleotide polymorphisms (SNPs) were genotyped by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The ADMA plasma levels was determined by ELISA and all biochemical indicators of serum were examined.
  
  
  RESULTS
  fifty-seven hemodialysis patients participated in our study, 54 (95.4%) of them had increased ADMA plasma levels. DNA sequencing analysis of 13 patients showed a suspected PRMT-1 gene polymorphism at sequence 5837 as there were different genotypes between C and G.
  
  
  CONCLUSIONS
  the increased levels of ADMA might be caused by PRMT-1 gene polymorphism.
authors: Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo Hospital, Surabaya, Indonesia.
authors: Mochammad
authors: M
authors: Thaha
authors: Wenny P
authors: WP
authors: Nilamsari
authors: Mochammad
authors: M
authors: Yusuf
authors: M
authors: M
authors: Amin
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics*; D006435:Renal Dialysis; D012097:Repressor Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D012097:Repressor Proteins; C487735:dimethylarginine; D001120:Arginine; C099988:PRMT1 protein, human; D011484:Protein-Arginine N-Methyltransferases