title: Faster, safer, and better DNA purification by ultracentrifugation using GelRed stain and development of mismatch oligo DNA for genome walking.
abstract: Purification of plant DNA involves lengthy ultracentrifugation using ethidium bromide. Here, ultracentrifugation method is improved by staining with GelRed. The resulting method is faster, safer and of higher sensitivity. Purified DNA quality was confirmed by treatment with restriction enzymes and isolation of gene promoters. New type of long adaptor with mismatch sequence was also developed for promoter isolation.
authors: a NARO Institute of Floricultural Science (NIFS) , National Agriculture and Food Research Organization (NARO) , Tsukuba , Japan.
authors: Ichiro
authors: I
authors: Kasajima
authors: Norihiro
authors: N
authors: Ohtsubo
authors: Katsutomo
authors: K
authors: Sasaki
mesh_terms: D034001:Adaptor Protein Complex Subunits / Q000737:chemistry; D020137:Base Pair Mismatch; D001483:Base Sequence; D004396:Coloring Agents / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification*; D023281:Genomics / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013997:Time Factors; D014461:Ultracentrifugation
chemical_list: D034001:Adaptor Protein Complex Subunits; D004396:Coloring Agents; D004247:DNA
keywords: DNA extraction; GelRed; ethidium bromide; ultracentrifugation