title: Biocompatibility of submicron Bioglass® powders obtained by a top-down approach.
abstract: In this study in vitro bioactivity and biocompatibility of two submicron 45S5 Bioglass® powders obtained by topdown processing have been evaluated and are compared to the as-received powder. Both submicron powders exhibited flake-like morphologies with lateral extensions of only a few microns; the flake thickness accounted for a few tens of nanometers. Enhanced in vitro bioactivity was found for the comminuted powders upon immersion in simulated body fluid. In vitro biocompatibility was evaluated by incubation of MG-63 osteoblast-like cells with various amounts (0–200 μg/mL) of the glass powders. Neither LDH-activity nor mitochondrial activity (WST-8) tests indicated cell toxicity. Increased mitochondrial activity was found for the submicron powders: incubation with high amounts revealed up to a threefold increase of osteoblast activity (ALP-activity). An overgrowth of the formed mineralized phase with phenotypical MG-63 cells was found by staining only for the submicron glasses. A distance ring is formed for the as-received powder. Superior bioactivity markers are found for shorter process times, that is, lower mass specific surface areas. This is attributed to the formation of carbonates during the comminution process.
authors: Anja
authors: A
authors: Dörfler
authors: Rainer
authors: R
authors: Detsch
authors: Stefan
authors: S
authors: Romeis
authors: Jochen
authors: J
authors: Schmidt
authors: Claudia
authors: C
authors: Eisermann
authors: Wolfgang
authors: W
authors: Peukert
authors: Aldo R
authors: AR
authors: Boccaccini
mesh_terms: D002460:Cell Line; D002516:Ceramics / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008422:Materials Testing; D008928:Mitochondria / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010316:Particle Size; D011208:Powders
chemical_list: C022107:Bioglass; D011208:Powders; D007770:L-Lactate Dehydrogenase