title: Immunological multimetal deposition for rapid visualization of sweat fingerprints.
abstract: A simple method termed immunological multimetal deposition (iMMD) was developed for rapid visualization of sweat fingerprints with bare eyes, by combining the conventional MMD with the immunoassay technique. In this approach, antibody-conjugated gold nanoparticles (AuNPs) were used to specifically interact with the corresponding antigens in the fingerprint residue. The AuNPs serve as the nucleation sites for autometallographic deposition of silver particles from the silver staining solution, generating a dark ridge pattern for visual detection. Using fingerprints inked with human immunoglobulin G (hIgG), we obtained the optimal formulation of iMMD, which was then successfully applied to visualize sweat fingerprints through the detection of two secreted polypeptides, epidermal growth factor and lysozyme. In comparison with the conventional MMD, iMMD is faster and can provide additional information than just identification. Moreover, iMMD is facile and does not need expensive instruments.
authors: Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058 (China) http://mypage.zju.edu.cn/binsu.
authors: Yayun
authors: Y
authors: He
authors: Linru
authors: L
authors: Xu
authors: Yu
authors: Y
authors: Zhu
authors: Qianhui
authors: Q
authors: Wei
authors: Meiqin
authors: M
authors: Zhang
authors: Bin
authors: B
authors: Su
mesh_terms: D003878:Dermatoglyphics; D004815:Epidermal Growth Factor / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D009113:Muramidase / Q000032:analysis; D061848:Optical Imaging; D010455:Peptides / Q000032:analysis; D016622:Silver Staining; D013542:Sweat / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D010455:Peptides; D004815:Epidermal Growth Factor; D006046:Gold; D009113:Muramidase
keywords: fingerprints; gold nanoparticles; immunology; multimetal deposition; polypeptides