title: MUC1 expression in fallopian tubes of women with hydrosalpinx.
abstract: OBJECTIVE
  To analyze the expression of MUC1 in Fallopian tubes with or without hydrosalpinx, using four different types of antibody.
  
  
  METHODS
  In a case-control study, immunohistochemical expression of MUC1 was examined in Fallopian tubes derived from women with hydrosalpinx (n=10) and normal controls (n=10). Four different antibodies were used for the detection of both extracellular (214D4, HMFG1, VPM654) and intracellular (EPR1023) MUC1 epitopes. Staining intensity was measured with ImageJ software. Expression of MUC1 mRNA was quantified by quantitative RT-PCR. Statistical analysis was performed with Student t-test (mean ± SD) and Mann-Whitney U-test (median [range]).
  
  
  RESULTS
  The mean (±SD) and median [range] intensity of MUC1 in controls vs. hydrosalpinx were: 214D4-67.5 ± 11.3 vs. 74.8 ± 14.69 (P=0.22); HMFG1-95.3 [642-1079] vs. 97.0 [502-1550] (P=0.91); VPM654-41.1 [314-914] vs. 46.0 [390-1424] (P=0.1); EPR1023-24.7 ± 7.3 vs. 57.4 ± 31.3 (P=0.01). MUC1 mRNA was 0.16 [008-05] vs. 0.09 [005-019] (P=0.06). Ectodomains and mRNA of MUC1 are unchanged in tubes from hydrosalpinx patients. In contrast, immunodetection of the MUC1 cytoplasmic tail is enhanced in tubes from hydrosalpinx.
  
  
  CONCLUSIONS
  Fallopian tubes with hydrosalpinx have a selective accumulation of MUC1 cytoplasmic tail, but not difference in the ectodomain.
authors: Departamento de Ginecologia e Obstetrícia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Wiess School of Natural Sciences Rice University, Department of Biochemistry and Molecular Biology, Anderson Cancer Center, Houston, TX, USA.
authors: Alice L
authors: AL
authors: Silva
authors: Departamento de Ginecologia e Obstetrícia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Wiess School of Natural Sciences Rice University, Department of Biochemistry and Molecular Biology, Anderson Cancer Center, Houston, TX, USA.
authors: Daniele G
authors: DG
authors: Fuhrich
authors: Departamento de Ginecologia e Obstetrícia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Wiess School of Natural Sciences Rice University, Department of Biochemistry and Molecular Biology, Anderson Cancer Center, Houston, TX, USA.
authors: Daniel D
authors: DD
authors: Carson
authors: Departamento de Ginecologia e Obstetrícia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Wiess School of Natural Sciences Rice University, Department of Biochemistry and Molecular Biology, Anderson Cancer Center, Houston, TX, USA.
authors: Brian J
authors: BJ
authors: Engel
authors: Departamento de Ginecologia e Obstetrícia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Wiess School of Natural Sciences Rice University, Department of Biochemistry and Molecular Biology, Anderson Cancer Center, Houston, TX, USA. Electronic address: rsavaris@hcpa.ufrgs.br.
authors: Ricardo F
authors: RF
authors: Savaris
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005184:Fallopian Tube Diseases / Q000235:genetics* / Q000378:metabolism; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D018396:Mucin-1 / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies
chemical_list: C060192:MUC1 protein, human; D018396:Mucin-1; D012333:RNA, Messenger
keywords: Hydrosalpinx; Immunohistochemistry; MUC1