title: Photoreceptor partner FHY1 has an independent role in gene modulation and plant development under far-red light.
abstract: To incorporate the far-red light (FR) signal into a strategy for optimizing plant growth, FAR-RED ELONGATED HYPOCOTYL1 (FHY1) mediates the nuclear translocation of the FR photoreceptor phytochrome A (phyA) and facilitates the association of phyA with the promoters of numerous associated genes crucial for the response to environmental stimuli. However, whether FHY1 plays additional roles after FR irradiation remains elusive. Here, through the global identification of FHY1 chromatin association sites through ChIP-seq analysis and by the comparison of FHY1-associated sites with phyA-associated sites, we demonstrated that nuclear FHY1 can either act independently of phyA or act in association with phyA to activate the expression of distinct target genes. We also determined that phyA can act independently of FHY1 in regulating phyA-specific target genes. Furthermore, we determined that the independent FHY1 nuclear pathway is involved in crucial aspects of plant development, as in the case of inhibited seed germination under FR during salt stress. Notably, the differential presence of cis-elements and transcription factors in common and unique FHY1- and/or phyA-associated genes are indicative of the complexity of the independent and coordinated FHY1 and phyA pathways. Our study uncovers previously unidentified aspects of FHY1 function beyond its currently recognized role in phyA-dependent photomorphogenesis.
authors: Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking-Qinghua Center of Life Science, College of Life Sciences, Peking University, Beijing 100871, China;Departments of Molecular, Cellular, and Developmental Biology and.
authors: Fang
authors: F
authors: Chen
authors: Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking-Qinghua Center of Life Science, College of Life Sciences, Peking University, Beijing 100871, China;Departments of Molecular, Cellular, and Developmental Biology and.
authors: Bosheng
authors: B
authors: Li
authors: Department of Plant Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada H9X 3V9.
authors: Jordan
authors: J
authors: Demone
authors: Department of Plant Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada H9X 3V9.
authors: Jean-Benoit
authors: JB
authors: Charron
authors: Pharmacology, Yale University, New Haven, CT 06520; and.
authors: Xiarong
authors: X
authors: Shi
authors: Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking-Qinghua Center of Life Science, College of Life Sciences, Peking University, Beijing 100871, China;Departments of Molecular, Cellular, and Developmental Biology and xingwang.deng@yale.edu.
authors: Xing Wang
authors: XW
authors: Deng
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D017343:Genes, Plant; D018525:Germination; D008027:Light; D008954:Models, Biological; D055519:Photoreceptors, Plant / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D010834:Phytochrome / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D051942:Phytochrome A / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D030821:Plants, Genetically Modified; D055049:Salt Tolerance; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; C441269:FHY1 protein, Arabidopsis; C497973:PHYA protein, Arabidopsis; D055519:Photoreceptors, Plant; D051942:Phytochrome A; D010834:Phytochrome