title: MicroRNA-145 is a potential prognostic factor of scirrhous type gastric cancer.
abstract: Gastric cancer (GC) is one of the most common malignancies worldwide. In particular, scirrhous type GC is highly metastatic and is characterized clinically by rapid disease progression and poor prognosis. MicroRNAs (miRNAs) play crucial roles in cancer development and progression. We previously demonstrated by microarray analysis that microRNA-145 (miR-145) is one of the more highly expressed miRNAs in scirrhous type GC vs. non-scirrhous types of GC. In the present study, we investigated the role of miR-145 in scirrhous type GC. The expression levels of miR-145 assessed by quantitative RT-PCR were higher in scirrhous type GC tissue samples than in non-scirrhous type GC and corresponding normal tissues. GC patients with high miR-145 expression were at a more advanced tumor stage (P=0.0156) and had more scirrhous type histology (P=0.0054) than those with low miR-145 expression. Furthermore, miR-145 expression was significantly associated with poor prognosis in GC patients (P=0.0438). miR-145 expression was localized in stromal fibroblasts of scirrhous type GC but not in cancer cells. miR-145 was induced by treatment by transforming growth factor-β, and it enhanced the expression of α-smooth muscle actin, a marker of myofibroblasts, in both normal gastric fibroblasts and cancer-associated fibroblasts. These data suggest that miR-145 may contribute to the progression of scirrhous type GC by regulating activation of peri-tumoral fibroblasts.
authors: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Minami‑ku, Hiroshima 734-8551, Japan.
authors: Yutaka
authors: Y
authors: Naito
authors: Faculty of Medicine, Hiroshima University, Minami‑ku, Hiroshima 734-8551, Japan.
authors: Kyohei
authors: K
authors: Yasuno
authors: Faculty of Medicine, Hiroshima University, Minami‑ku, Hiroshima 734-8551, Japan.
authors: Hiroko
authors: H
authors: Tagawa
authors: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Minami‑ku, Hiroshima 734-8551, Japan.
authors: Naoya
authors: N
authors: Sakamoto
authors: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Minami‑ku, Hiroshima 734-8551, Japan.
authors: Naohide
authors: N
authors: Oue
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Abeno-ku, Osaka 545-8585, Japan.
authors: Masakazu
authors: M
authors: Yashiro
authors: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Minami‑ku, Hiroshima 734-8551, Japan.
authors: Kazuhiro
authors: K
authors: Sentani
authors: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Minami‑ku, Hiroshima 734-8551, Japan.
authors: Keisuke
authors: K
authors: Goto
authors: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Minami‑ku, Hiroshima 734-8551, Japan.
authors: Shunsuke
authors: S
authors: Shinmei
authors: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Minami‑ku, Hiroshima 734-8551, Japan.
authors: Htoo Zarni
authors: HZ
authors: Oo
authors: Division of Translational Research, Exploratory Oncology and Clinical Trial Center, National Cancer Center, Kashiwa, Chiba 277-8577, Japan.
authors: Kazuyoshi
authors: K
authors: Yanagihara
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Abeno-ku, Osaka 545-8585, Japan.
authors: Kosei
authors: K
authors: Hirakawa
authors: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Minami‑ku, Hiroshima 734-8551, Japan.
authors: Wataru
authors: W
authors: Yasui
mesh_terms: D002293:Adenocarcinoma, Scirrhous / Q000235:genetics* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C541010:MIRN145 microRNA, human; D035683:MicroRNAs