title: Resveratrol ameliorates motor neuron degeneration and improves survival in SOD1(G93A) mouse model of amyotrophic lateral sclerosis.
abstract: Resveratrol has recently been used as a supplemental treatment for several neurological and nonneurological diseases. It is not known whether resveratrol has neuroprotective effect on amyotrophic lateral sclerosis (ALS). To assess the effect of resveratrol on the disease, we tested this agent on an ALS model of SOD1(G93A) transgenic mouse. Rotarod measurement was performed to measure the motor function of the ALS mice. Nissl staining and SMI-32 immunofluorescent staining were used to determine motor neurons survival in the spinal cord of the ALS mice. Hematoxylin-eosin (H&E), succinic dehydrogenase (SDH), and cytochrome oxidase (COX) staining were applied to pathologically analyze the skeletal muscles of the ALS mice. We found that resveratrol treatment significantly delayed the disease onset and prolonged the lifespan of the ALS mice. Furthermore, resveratrol treatment attenuated motor neuron loss, relieved muscle atrophy, and improved mitochondrial function of muscle fibers in the ALS mice. In addition, we demonstrated that resveratrol exerted these neuroprotective effects mainly through increasing the expression of Sirt1, consequently suppressing oxidative stress and downregulating p53 and its related apoptotic pathway. Collectively, our findings suggest that resveratrol might provide a promising therapeutic intervention for ALS.
authors: Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Ruijin 2nd Road No. 197, Building 11-1201, Shanghai 200025, China.
authors: Lin
authors: L
authors: Song
authors: Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Ruijin 2nd Road No. 197, Building 11-1201, Shanghai 200025, China.
authors: Liang
authors: 0000-0003-0554-2733
authors: L
authors: Chen
authors: The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors: Xiaojie
authors: X
authors: Zhang
authors: The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors: Jia
authors: J
authors: Li
authors: Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Ruijin 2nd Road No. 197, Building 11-1201, Shanghai 200025, China ; Center for Translational Research of Neurology Disease, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
authors: Weidong
authors: W
authors: Le
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D017209:Apoptosis; D001522:Behavior, Animal; D004195:Disease Models, Animal; D005260:Female; D015227:Lipid Peroxidation; D008159:Lumbar Vertebrae / Q000473:pathology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D009046:Motor Neurons / Q000187:drug effects* / Q000473:pathology; D018482:Muscle, Skeletal / Q000473:pathology; D019636:Neurodegenerative Diseases / Q000188:drug therapy*; D018384:Oxidative Stress; D000077185:Resveratrol; D013116:Spinal Cord / Q000473:pathology; D013267:Stilbenes / Q000737:chemistry / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000235:genetics*; D000072105:Superoxide Dismutase-1; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D016159:Tumor Suppressor Protein p53; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000077185:Resveratrol