title: Pancreatic ductal perfusion at organ procurement enhances islet yield in human islet isolation.
abstract: OBJECTIVE
  Pancreas preservation is a major factor influencing the results of islet cell transplantation. This study evaluated the effects of 2 different solutions for pancreatic ductal perfusion (PDP) at organ procurement.
  
  
  METHODS
  Eighteen human pancreases were assigned to 3 groups: non-PDP (control), PDP with ET-Kyoto solution, and PDP with cold storage/purification stock solution. Pancreatic islets were isolated according to the modified Ricordi method.
  
  
  RESULTS
  No significant differences in donor characteristics, including cold ischemia time, were observed between the 3 groups. All islet isolations in the PDP groups had more than 400,000 islet equivalence in total islet yield after purification, a significant increase when compared with the control (P = 0.04 and P < 0.01). The islet quality assessments, including an in vivo diabetic nude mice assay and the response of high-mobility group box protein 1 to cytokine stimulation, also showed no significant differences. The proportion of terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive cells showing apoptosis in islets in the PDP groups was significantly lower than in the control group (P < 0.05).
  
  
  CONCLUSIONS
  Both ET-Kyoto solution and cold storage/purification stock solution are suitable for PDP and consistently resulted in isolation success. Further studies with a larger number of pancreas donors should be done to compare the effects of the PDP solutions.
authors: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
authors: Morihito
authors: M
authors: Takita
authors: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
authors: Takeshi
authors: T
authors: Itoh
authors: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
authors: Masayuki
authors: M
authors: Shimoda
authors: Institute for Biomedical Studies, Baylor University, Waco, Texas.
authors: Mazhar A
authors: MA
authors: Kanak
authors: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
authors: Rauf
authors: R
authors: Shahbazov
authors: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
authors: Faisal
authors: F
authors: Kunnathodi
authors: Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
authors: Michael C
authors: MC
authors: Lawrence
authors: Baylor Annette C. and Harold C. Simmons Transplant Institute, Baylor University Medical Center, Dallas, Texas.
authors: Bashoo
authors: B
authors: Naziruddin
authors: Baylor Annette C. and Harold C. Simmons Transplant Institute, Baylor University Medical Center, Dallas, Texas.
authors: Marlon F
authors: MF
authors: Levy
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis; D002452:Cell Count; D002469:Cell Separation; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D005260:Female; D005942:Gluconates / Q000494:pharmacology; D024243:HMGB1 Protein / Q000494:pharmacology; D064593:Heterografts; D006639:Histidine / Q000494:pharmacology; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology; D007515:Islets of Langerhans / Q000187:drug effects; D016381:Islets of Langerhans Transplantation; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010183:Pancreatic Ducts / Q000166:cytology*; D010477:Perfusion; D010710:Phosphates / Q000494:pharmacology; D011446:Prospective Studies; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014199:Trehalose / Q000494:pharmacology; D055815:Young Adult
chemical_list: D016207:Cytokines; C087059:ET-Kyoto solution; D005942:Gluconates; D024243:HMGB1 Protein; D006895:Hydroxyethyl Starch Derivatives; D019188:Organ Preservation Solutions; D010710:Phosphates; D006639:Histidine; D014199:Trehalose