title: Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in sheep.
abstract: Regenerative therapies using adult stem cells have attracted great interest in the recent years and offer a promising alternative to current surgical practices. In this report, we evaluated the safety and efficacy of an autologous cell-based treatment of osteoarthritis using mesenchymal stromal cells expanded from bone marrow aspirates that were administered intra-articularly. Ten 2-year old ewes were divided in two groups (for analysis at 6 and 12 months, respectively). Full thickness articular cartilage defects of approximately 60mm(2) were created arthroscopically in the medial femorotibial condyles and a meniscal tear in the anterior horn of the medial meniscus in the 20 hind legs. Intra-articular injection of 4 mL of either treatment (a suspension of cells) or control (same as treatment, without cells) were applied one month after generating a chronic condition similar to human pathology. Animals were monitored radiographically, by MRI and ultrasound scanning; and macroscopic and histological analyses were conducted at 6 and 12 months. Furthermore a full necropsy was performed at 12 months post-treatment. The intra-articular injection of autologous MSC was safe, as judged by the lack of local or systemic adverse effects during the clinical follow-up and by a full necropsy performed at 12 months post-treatment. Evidence of regeneration of articular cartilage and meniscus was case-dependent but statistically significant improvement was found in specific macroscopic and histological parameters. Such parameters included colour, rigidity, cell distribution and hyaline quality of the refill tissue as well as the structure of subchondral bone.
authors: Divisió de Teràpies Avançades/XCELIA, Banc de Sang i Teixits, Edifici Dr. Frederic Duran i Jordà, Passeig Taulat, 116, Barcelona 08005, Spain.
authors: Marta
authors: M
authors: Caminal
authors: Departament de Medicina i Cirurgia Animals, Àrea de Medicina i Cirurgia Animal, Facultat de Veterinària, Universitat Autònoma de Barcelona, Edifici V, Campus de la UAB, 08193 Bellaterra, Cerdanyola del Vallès, Spain.
authors: Carla
authors: C
authors: Fonseca
authors: Grup d'Enginyeria Cel·lular i Tissular, Departament d'Enginyeria Química, Escola d'Enginyers, Universitat Autònoma de Barcelona, Edifici Q, Campus de la UAB, 08193 Bellaterra, Cerdanyola del Vallès, Spain.
authors: David
authors: D
authors: Peris
authors: Departament de Medicina i Cirurgia Animals, Àrea de Medicina i Cirurgia Animal, Facultat de Veterinària, Universitat Autònoma de Barcelona, Edifici V, Campus de la UAB, 08193 Bellaterra, Cerdanyola del Vallès, Spain.
authors: Xavier
authors: X
authors: Moll
authors: Departament de Medicina i Cirurgia Animals, Àrea de Medicina i Cirurgia Animal, Facultat de Veterinària, Universitat Autònoma de Barcelona, Edifici V, Campus de la UAB, 08193 Bellaterra, Cerdanyola del Vallès, Spain.
authors: Rosa M
authors: RM
authors: Rabanal
authors: Hospital ASEPEYO, Avinguda Alcalde Barnils, 54-60, Sant Cugat del Vallès, Barcelona 08174, Spain.
authors: Josep
authors: J
authors: Barrachina
authors: Hospital ASEPEYO, Avinguda Alcalde Barnils, 54-60, Sant Cugat del Vallès, Barcelona 08174, Spain.
authors: David
authors: D
authors: Codina
authors: Departament de Medicina i Cirurgia Animals, Àrea de Medicina i Cirurgia Animal, Facultat de Veterinària, Universitat Autònoma de Barcelona, Edifici V, Campus de la UAB, 08193 Bellaterra, Cerdanyola del Vallès, Spain.
authors: Félix
authors: F
authors: García
authors: Grup d'Enginyeria Cel·lular i Tissular, Departament d'Enginyeria Química, Escola d'Enginyers, Universitat Autònoma de Barcelona, Edifici Q, Campus de la UAB, 08193 Bellaterra, Cerdanyola del Vallès, Spain.
authors: Jordi J
authors: JJ
authors: Cairó
authors: Grup d'Enginyeria Cel·lular i Tissular, Departament d'Enginyeria Química, Escola d'Enginyers, Universitat Autònoma de Barcelona, Edifici Q, Campus de la UAB, 08193 Bellaterra, Cerdanyola del Vallès, Spain.
authors: Francesc
authors: F
authors: Gòdia
authors: Divisió de Teràpies Avançades/XCELIA, Banc de Sang i Teixits, Edifici Dr. Frederic Duran i Jordà, Passeig Taulat, 116, Barcelona 08005, Spain.
authors: Arnau
authors: A
authors: Pla
authors: Divisió de Teràpies Avançades/XCELIA, Banc de Sang i Teixits, Edifici Dr. Frederic Duran i Jordà, Passeig Taulat, 116, Barcelona 08005, Spain. Electronic address: jvives@bst.cat.
authors: Joaquim
authors: J
authors: Vives
mesh_terms: D000818:Animals; D064592:Autografts; D002358:Cartilage, Articular / Q000000981:diagnostic imaging / Q000293:injuries*; D002908:Chronic Disease; D004195:Disease Models, Animal; D006801:Humans; D007718:Knee Injuries / Q000000981:diagnostic imaging / Q000628:therapy*; D008279:Magnetic Resonance Imaging; D008592:Menisci, Tibial / Q000000981:diagnostic imaging; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D011859:Radiography; D012756:Sheep; D000070600:Tibial Meniscus Injuries