title: Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1β expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro.
abstract: In humans, usual aging, differently from successful aging, is associated with deregulation of proinflammatory/anti-inflammatory cytokine balance. The corresponding data from rat studies are limited. Therefore, we examined (i) cytokine messenger RNA (mRNA) profile of fresh peritoneal cells from 6- (adult), 24- (old), and 31-month-old (long-lived) AO rats and (ii) proinflammatory (IL-1β and IL-6) and anti-inflammatory (IL-10) cytokine, NO, and urea production in their LPS-stimulated cultures. Comparing with adult rats, cells from old ones expressed lower amount of TNF-α and IL-6 mRNAs, but greater amount of IL-1β mRNA. On the other hand, cells from long-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-α and IL-6 mRNA expression, and comparable expression of IL-1β mRNA relative to adult rats. Consequently, IL-10/IL-1β mRNA ratio was greater in cells from long-lived rats than in adult and old rats. In LPS-stimulated peritoneal cell cultures (contained ≥95 % macrophages) from old rats, concentration of common proinflammatory cytokines was higher than in those from adult rats. Comparing with adult and old rats, in LPS-stimulated macrophage cultures from long-lived rats, TNF-α and IL-6 concentrations were lower; IL-1β concentration was comparable or greater (in respect to adult rats), whereas that of IL-10 was strikingly higher. Consistently, in macrophage cultures from long-lived rats, NO (iNOS activity marker)/urea (arginase activity marker) ratio was less and not different from that in old and adult rats, respectively. The study suggests that macrophages from long-lived rats, differently from those of old ones, have substantial ability to limit proinflammatory mediator production, which may contribute to their longevity.
authors: Institute of Virology, Vaccines and Sera, "Torlak", Immunology Research Center "Branislav Janković", Vojvode Stepe 458, 11152, Belgrade, Serbia, miradim@sezampro.rs.
authors: Mirjana
authors: M
authors: Dimitrijević
authors: Iva
authors: I
authors: Aleksić
authors: Vesna
authors: V
authors: Vujić
authors: Stanislava
authors: S
authors: Stanojević
authors: Ivan
authors: I
authors: Pilipović
authors: Stephan
authors: S
authors: von Hörsten
authors: Gordana
authors: G
authors: Leposavić
mesh_terms: D000375:Aging; D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism / Q000473:pathology*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D005786:Gene Expression Regulation; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics*; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics*; D009569:Nitric Oxide / Q000494:pharmacology; D010538:Peritonitis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000494:pharmacology
chemical_list: D053583:Interleukin-1beta; D016753:Interleukin-10; D009569:Nitric Oxide; D012313:RNA; D014508:Urea