title: Antibodies to DNA repair proteins in headache with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL) patients.
abstract: Autoimmune mechanisms have been implicated in the pathogenesis of headache and neurological deficits with cerebrospinal fluid lymphocytosis (HaNDL). Pooled sera of five HaNDL patients and 30 controls (10 multiple sclerosis patients, 10 migraine patients, 10 healthy controls) were screened by protein macroarray. All sera were also individually subjected to immunoprecipitation with neuroblastoma cells and the bound antigens were identified by mass spectrometry. Antibodies to three DNA repair proteins (mitogen-activated protein kinase-4, DNA-dependent protein kinase catalytic subunit, DNA excision repair protein ERCC-6) were identified by both macroarray and immunoprecipitation methods in 3/5 HaNDL sera, but in none of the controls. The presence of DNA repair protein antibodies indicates DNA damage and provides further support for the inflammatory etiology of HaNDL.
authors: Department of Neuroscience, Institute for Experimental Medicine, Istanbul University, Çapa, 34390, Istanbul, Turkey.
authors: Ece
authors: E
authors: Erdağ
authors: Neşe
authors: N
authors: Çelebisoy
authors: Ayşe Nur
authors: AN
authors: Yüceyar
authors: Murat
authors: M
authors: Kürtüncü
authors: Burçak
authors: B
authors: Vural
authors: Erdem
authors: E
authors: Tüzün
mesh_terms: D000328:Adult; D000906:Antibodies / Q000097:blood*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004265:DNA Helicases / Q000276:immunology; D045643:DNA Repair Enzymes / Q000276:immunology; D051747:DNA-Activated Protein Kinase / Q000276:immunology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000276:immunology; D005260:Female; D006261:Headache / Q000209:etiology*; D006801:Humans; D047468:Immunoprecipitation; D008218:Lymphocytosis / Q000150:complications*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000150:complications; D009422:Nervous System Diseases / Q000150:complications*; D009447:Neuroblastoma / Q000473:pathology; D000075223:Poly-ADP-Ribose Binding Proteins; D040081:Protein Array Analysis; D055815:Young Adult
chemical_list: D000906:Antibodies; D000075223:Poly-ADP-Ribose Binding Proteins; D051747:DNA-Activated Protein Kinase; D048049:Extracellular Signal-Regulated MAP Kinases; C102548:Mapk4 protein, rat; D004265:DNA Helicases; C078673:ERCC6 protein, human; D045643:DNA Repair Enzymes