title: Osteogenic efficacy of bone marrow concentrate in rabbit maxillary sinus grafting.
abstract: Maxillary sinus grafting is required to increase bone volume in the atrophic posterior maxilla to facilitate dental implant placement. Grafting with autogenous bone (AB) is ideal, but additional bone harvesting surgery is unpleasant. Alternatively, bone substitutes have been used but they limit new bone formation. The strategy of single-visit clinical stem cell therapy using bone marrow aspirate concentrate (BMAC) to facilitate new bone formation has been proposed. This study aimed to assess bone regeneration capacity of autologous BMAC mixed with bovine bone mineral (BBM) in maxillary sinus grafting. Twenty-four white New Zealand rabbits were used and their maxillary sinuses were randomly assigned for grafting with 4 different materials. Rates of new bone apposition in augmented sinuses were measured and bone histomorphometry were examined. Significant increase in the quantity of nucleated cells and colony forming unit-fibroblasts were confirmed in BMAC. Mesenchymal stem cells in BMAC retained their in vitro multi-differentiation capability. Higher rates of mineral appositions in the early period were detected in BBM + BMAC and AB than BBM alone, though they are not significantly different. Graft volume/tissue volumes in BBM and BBM + BMAC were found to be higher than those in AB and sham.
authors: Oral and Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, China; Faculty of Dentistry, Thammasat University, Thailand.
authors: L
authors: L
authors: Sununliganon
authors: Oral and Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, China.
authors: L
authors: L
authors: Peng
authors: Faculty of Dentistry, Thammasat University, Thailand.
authors: W
authors: W
authors: Singhatanadgit
authors: Oral and Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, China. Electronic address: lkcheung@hku.hk.
authors: L K
authors: LK
authors: Cheung
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000818:Animals; D064592:Autografts / Q000473:pathology / Q000637:transplantation; D016026:Bone Marrow Transplantation / Q000379:methods*; D001861:Bone Regeneration / Q000502:physiology; D018786:Bone Substitutes / Q000627:therapeutic use; D002417:Cattle; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D020219:Chondrogenesis / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D005456:Fluorescent Dyes; D008443:Maxillary Sinus / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D039902:Multipotent Stem Cells / Q000637:transplantation; D010012:Osteogenesis / Q000502:physiology*; D019152:Paracentesis / Q000379:methods; D011817:Rabbits; D011897:Random Allocation; D059546:Sinus Floor Augmentation / Q000379:methods*; D013997:Time Factors; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D018786:Bone Substitutes; D005456:Fluorescent Dyes
keywords: Bone augmentation; Bone marrow aspirate concentrate; Maxillary sinus grafting; Rabbit