title: Similar is not the same: differences in the function of the (hemi-)cellulolytic regulator XlnR (Xlr1/Xyr1) in filamentous fungi.
abstract: The transcriptional activator XlnR (Xlr1/Xyr1) is a major regulator in fungal xylan and cellulose degradation as well as in the utilization of d-xylose via the pentose catabolic pathway. XlnR homologs are commonly found in filamentous ascomycetes and often assumed to have the same function in different fungi. However, a comparison of the saprobe Aspergillus niger and the plant pathogen Magnaporthe oryzae showed different phenotypes for deletion strains of XlnR. In this study wild type and xlnR/xlr1/xyr1 mutants of five fungi were compared: Fusarium graminearum, M. oryzae, Trichoderma reesei, A. niger and Aspergillus nidulans. Growth profiling on relevant substrates and a detailed analysis of the secretome as well as extracellular enzyme activities demonstrated a common role of this regulator in activating genes encoding the main xylanolytic enzymes. However, large differences were found in the set of genes that is controlled by XlnR in the different species, resulting in the production of different extracellular enzyme spectra by these fungi. This comparison emphasizes the functional diversity of a fine-tuned (hemi-)cellulolytic regulatory system in filamentous fungi, which might be related to the adaptation of fungi to their specific biotopes. Data are available via ProteomeXchange with identifier PXD001190.
authors: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; Fungal Molecular Physiology, Utrecht University, Utrecht, The Netherlands.
authors: Sylvia
authors: S
authors: Klaubauf
authors: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.
authors: Hari Mander
authors: HM
authors: Narang
authors: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands; Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors: Harm
authors: H
authors: Post
authors: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; Fungal Molecular Physiology, Utrecht University, Utrecht, The Netherlands.
authors: Miaomiao
authors: M
authors: Zhou
authors: Institute of Chemical Engineering, Department for Biotechnology and Microbiology, Vienna University of Technology, Gumpendorferstr. 1a, 1060 Vienna, Austria.
authors: Kurt
authors: K
authors: Brunner
authors: Institute of Chemical Engineering, Department for Biotechnology and Microbiology, Vienna University of Technology, Gumpendorferstr. 1a, 1060 Vienna, Austria.
authors: Astrid R
authors: AR
authors: Mach-Aigner
authors: Institute of Chemical Engineering, Department for Biotechnology and Microbiology, Vienna University of Technology, Gumpendorferstr. 1a, 1060 Vienna, Austria.
authors: Robert L
authors: RL
authors: Mach
authors: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands; Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors: Albert J R
authors: AJR
authors: Heck
authors: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands; Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors: A F Maarten
authors: AFM
authors: Altelaar
authors: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; Fungal Molecular Physiology, Utrecht University, Utrecht, The Netherlands. Electronic address: r.devries@cbs.knaw.nl.
authors: Ronald P
authors: RP
authors: de Vries
mesh_terms: D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000378:metabolism; D005658:Fungi / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D020543:Proteome / Q000032:analysis; D018085:Regulon; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D020543:Proteome; D015534:Trans-Activators; D014990:Xylans
keywords: Aspergillus; Fusarium graminearum; Magnaporthe oryzae; Trichoderma reesei; XlnR; Xylan degradation