title: Development of an experimental model of portal vein ligation associated with parenchymal transection (ALPPS) in rats.
abstract: BACKGROUND
  Liver failure might be a cause of death after major hepatectomies. The ALPPS technique appears to be a promising strategy to avoid it, however no experimental studies supporting this procedure have been previously described. The aim was to develop an experimental model of ALPPS in rats.
  
  
  METHODS
  Experimental. A total of 30 Sprague Dawley rats were used. To develop the ALPPS procedure, ligation of the left portal branch of the middle lobe (LM) was performed. This demarcates the left side (SILM) from the right side (SDLM); parenchyma transection was performed following the demarcated line. The animal's weight, volume and weight of both LM were analyzed. Sacrifice at 3, 7 and 14 days after the procedure (10 per group) was performed.
  
  
  RESULTS
  No bleeding or ascites were observed during the postoperative period. The LM increased by 24.1, 86.9 and 120.4% at 3, 7 and 14 days. The SDLM increased by 34.4, 78.8 and 102.0% at 3, 7 and 14 days. The SILM decreased 42.6, 64.8, and 79.3% at day 3, 7 and 14 days respectively.
  
  
  CONCLUSIONS
  The ALPPS procedure can be performed in rats, achieving the expected results. Comparison studies to 2 staged hepatectomy will be necessary.
authors: Instituto de Trasplante Multiorgánico, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina.
authors: Héctor M
authors: HM
authors: Almau Trenard
authors: Instituto de Trasplante Multiorgánico, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina.
authors: Luis E
authors: LE
authors: Moulin
authors: Instituto de Trasplante Multiorgánico, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina.
authors: Juan M
authors: JM
authors: Padín
authors: Laboratorio de Microcirugía Experimental, CONICET, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina.
authors: Pablo
authors: P
authors: Stringa
authors: Instituto de Trasplante Multiorgánico, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina; Laboratorio de Microcirugía Experimental, CONICET, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina.
authors: Gabriel E
authors: GE
authors: Gondolesi
authors: Instituto de Trasplante Multiorgánico, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina. Electronic address: pbarros@ffavaloro.org.
authors: Pablo
authors: P
authors: Barros Schelotto
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D058851:GPI-Linked Proteins; D006498:Hepatectomy / Q000379:methods*; D007527:Isoenzymes; D008026:Ligation; D008297:Male; D023421:Models, Animal; D008962:Models, Theoretical; D011169:Portal Vein / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D058851:GPI-Linked Proteins; D007527:Isoenzymes; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental
keywords: ALPPS; Hepatectomy; Hepatectomía; Hepatectomía en 2 tiempos; Two-stage hepatectomy