title: Epitope mapping of epidermal growth factor receptor (EGFR) monoclonal antibody and induction of growth-inhibitory polyclonal antibodies by vaccination with EGFR mimotope.
abstract: One of the proposed approaches in cancer therapy is to induce and direct the patient's own immune system against cancer cells. In this study, we determined the epitope mapping of the rat anti-human epidermal growth factor receptor (EGFR) monoclonal antibody ICR-62 using a phage display of random peptide library and identified a 12 amino acids peptide, which was recognized as a mimotope. The peptide was synthesized and conjugated to bovine serum albumin (BSA) as carrier protein (P-BSA). We have shown that ICR-62 can react specifically with P-BSA as well as native EGFR. Two rabbits were immunized either by BSA or P-BSA and the rabbits IgGs were purified and examined for binding to the antigens, mimotope and the EGFR protein purified from the EGFR overexpressing A431 cell line. We showed that the rabbit IgG generated against the mimotope is capable of inhibiting the growth of A431 cells by 15%, but does not have any effect on the growth of EGFR-negative MDA-MB-453 cell line in vitro. Our results support the need for further investigations on the potential of vaccination with either mimotope of the EGFR or epitope displayed on the surface of phage particles for use in active immunotherapy of cancer.
authors: Department of Medical Biotechnology, School of Medical Sciences, Tarbiat Modares University , Tehran , Islamic Republic of Iran and.
authors: Mohsen
authors: M
authors: Navari
authors: Mehrak
authors: M
authors: Zare
authors: Masoud
authors: M
authors: Javanmardi
authors: Majid
authors: M
authors: Asadi-Ghalehni
authors: Helmout
authors: H
authors: Modjtahedi
authors: Mohammad Javed
authors: MJ
authors: Rasaee
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000912:Antibodies, Neoplasm / Q000096:biosynthesis / Q000494:pharmacology*; D019496:Cancer Vaccines / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D018604:Epitope Mapping; D066246:ErbB Receptors / Q000276:immunology*; D005260:Female; D006801:Humans; D019151:Peptide Library; D011817:Rabbits; D012710:Serum Albumin, Bovine; D014611:Vaccination
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D019496:Cancer Vaccines; D019151:Peptide Library; D012710:Serum Albumin, Bovine; D066246:ErbB Receptors
keywords: Cancer vaccine; EGFR; immunotherapy; mimotope; phage display