title: Dietary heat-killed Lactobacillus brevis SBC8803 promotes voluntary wheel-running and affects sleep rhythms in mice.
abstract: OBJECTIVE
  We previously reported that heat-killed Lactobacillus brevis SBC8803 enhances appetite via changes in autonomic neurotransmission. Here we assessed whether a diet supplemented with heat-killed SBC8803 affects circadian locomotor rhythmicity and sleep architecture.
  
  
  RESULTS
  Daily total activity gradually increased in mice over 4 weeks and supplementation with heat-killed SBC8803 significantly intensified the increase, which reached saturation at 25 days. Electroencephalography revealed that SBC8803 supplementation significantly reduced the total amount of time spent in non-rapid eye movement (NREM) sleep and increased the amount of time spent being awake during the latter half of the nighttime, but tended to increase the total amount of time spent in NREM sleep during the daytime. Dietary supplementation with SBC8803 can extend the duration of activity during the nighttime and of sleep during the daytime. Daily voluntary wheel-running and sleep rhythmicity become intensified when heat-killed SBC8803 is added to the diet.
  
  
  CONCLUSIONS
  Dietary heat-killed SBC8803 can modulate circadian locomotion and sleep rhythms, which might benefit individuals with circadian rhythms that have been disrupted by stress or ageing.
authors: Biological Clock Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan. Electronic address: k-miyazaki@aist.go.jp.
authors: Koyomi
authors: K
authors: Miyazaki
authors: Biological Clock Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan.
authors: Nanako
authors: N
authors: Itoh
authors: Biological Clock Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan.
authors: Saori
authors: S
authors: Yamamoto
authors: Biological Clock Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan.
authors: Sayaka
authors: S
authors: Higo-Yamamoto
authors: Frontier Laboratories of Value Creation, Sapporo Breweries Ltd., Yaizu 325-0013, Japan.
authors: Yasukazu
authors: Y
authors: Nakakita
authors: Corporate Planning Department, Sapporo Holdings Ltd., Tokyo 150-8522, Japan.
authors: Hirotaka
authors: H
authors: Kaneda
authors: Frontier Laboratories of Value Creation, Sapporo Breweries Ltd., Yaizu 325-0013, Japan.
authors: Tatsuro
authors: T
authors: Shigyo
authors: Biological Clock Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan; Department of Medical Genome Science, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa 277-8561, Japan; Department of Applied Biological Science, Graduate School of Science and Technology, Tokyo University of Science, Noda 278-8510, Japan.
authors: Katsutaka
authors: K
authors: Oishi
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology; D002940:Circadian Rhythm / Q000187:drug effects / Q000502:physiology; D004032:Diet; D019587:Dietary Supplements; D004569:Electroencephalography; D052196:Levilactobacillus brevis / Q000378:metabolism*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009043:Motor Activity / Q000187:drug effects* / Q000502:physiology; D019936:Probiotics / Q000494:pharmacology; D012890:Sleep / Q000187:drug effects* / Q000502:physiology; D012894:Sleep Stages / Q000187:drug effects / Q000502:physiology; D014851:Wakefulness / Q000187:drug effects / Q000502:physiology
keywords: Bacteria; Biogenic-like supplements; Electroencephalography; Gut–brain axis