title: Effect of processing on physicochemical characteristics and bioefficacy of β-lactoglobulin-epigallocatechin-3-gallate complexes.
abstract: Varying amounts of epigallocatechin-3-gallate (EGCG) were encapsulated in β-lactoglobulin (β-Lg) nanoparticles, either native or processed, denoted as heated or desolvated protein. The stability, physical properties, and bioactivity of the β-Lg-EGCG complexes were tested. Native β-Lg-EGCG complexes showed comparable stability and binding efficacy (EGCG/β-Lg molar ratio of 1:1) to heated β-Lg nanoparticles (1% and 5% protein w/w). The sizes of heated and desolvated β-Lg nanoparticles were comparable, but the latter showed the highest binding affinity for EGCG. The presence of EGCG complexed with β-Lg did not affect the interfacial tension of the protein when tested at the soy oil-water interface but caused a decrease in dilational elasticity. All β-Lg complexes (native, heated, or desolvated) showed a decrease in cellular proliferation similar to that of free ECGC. In summary, protein-EGCG complexes did not alter the bioefficacy of EGCG and contributed to increased stability with storage, demonstrating the potential benefits of nanoencapsulation.
authors: Department of Food Science, University of Guelph , 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada.
authors: Pauline
authors: P
authors: Lestringant
authors: Anilda
authors: A
authors: Guri
authors: Ibrahim
authors: I
authors: Gülseren
authors: Perla
authors: P
authors: Relkin
authors: Milena
authors: M
authors: Corredig
mesh_terms: D018938:Caco-2 Cells; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004355:Drug Stability; D006358:Hot Temperature; D006801:Humans; D007782:Lactoglobulins / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D013500:Surface Tension
chemical_list: D007782:Lactoglobulins; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Caco-2 cells; EGCG; bioefficacy; fluorescence spectroscopy; interfacial tensiometry; nanoencapsulation; β-lactoglobulin