title: A novel liquid chromatography-tandem mass spectrometry method for determination of menadione in human plasma after derivatization with 3-mercaptopropionic acid.
abstract: Menadione (VK3), an essential fat-soluble naphthoquinone, takes very important physiological and pathological roles, but its detection and quantification is challenging. Herein, a new method was developed for quantification of VK3 in human plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS) after derivatization with 3-mercaptopropionic acid via Michael addition reaction. The derivative had been identified by the mass spectra and the derivatization conditions were optimized by considering different parameters. The method was demonstrated with high sensitivity and a low limit of quantification of 0.03 ng mL(-1) for VK3, which is about 33-fold better than that for the direct analysis of the underivatized compound. The method also had good precision and reproducibility. It was applied in the determination of basal VK3 in human plasma and a clinical pharmacokinetic study of menadiol sodium diphosphate. Furthermore, the method for the quantification of VK3 using LC-MS/MS was reported in this paper for the first time, and it will provide an important strategy for the further research on VK3 and menadione analogs.
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
authors: Ruijuan
authors: R
authors: Liu
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
authors: Mengmeng
authors: M
authors: Wang
authors: Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China. Electronic address: dinglidl@hotmail.com.
authors: Li
authors: L
authors: Ding
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000737:chemistry*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D015394:Molecular Structure; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D024483:Vitamin K 3 / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D014815:Vitamins / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D014815:Vitamins; D024483:Vitamin K 3; D015097:3-Mercaptopropionic Acid
keywords: 3-mercaptopropionic acid; Derivatization; Liquid chromatography-tandem mass spectrometry; Menadione; Michael addition; pharmacokinetic