title: Insights from molecular dynamics simulations: structural basis for the V567D mutation-induced instability of zebrafish alpha-dystroglycan and comparison with the murine model.
abstract: A missense amino acid mutation of valine to aspartic acid in 567 position of alpha-dystroglycan (DG), identified in dag1-mutated zebrafish, results in a reduced transcription and a complete absence of the protein. Lacking experimental structural data for zebrafish DG domains, the detailed mechanism for the observed mutation-induced destabilization of the DG complex and membrane damage, remained unclear. With the aim to contribute to a better clarification of the structure-function relationships featuring the DG complex, three-dimensional structural models of wild-type and mutant (V567D) C-terminal domain of alpha-DG from zebrafish were constructed by a template-based modelling approach. We then ran extensive molecular dynamics (MD) simulations to reveal the structural and dynamic properties of the C-terminal domain and to evaluate the effect of the single mutation on alpha-DG stability. A comparative study has been also carried out on our previously generated model of murine alpha-DG C-terminal domain including the I591D mutation, which is topologically equivalent to the V567D mutation found in zebrafish. Trajectories from MD simulations were analyzed in detail, revealing extensive structural disorder involving multiple beta-strands in the mutated variant of the zebrafish protein whereas local effects have been detected in the murine protein. A biochemical analysis of the murine alpha-DG mutant I591D confirmed a pronounced instability of the protein. Taken together, the computational and biochemical analysis suggest that the V567D/I591D mutation, belonging to the G beta-strand, plays a key role in inducing a destabilization of the alpha-DG C-terminal Ig-like domain that could possibly affect and propagate to the entire DG complex. The structural features herein identified may be of crucial help to understand the molecular basis of primary dystroglycanopathies.
authors: Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy.
authors: Davide
authors: D
authors: Pirolli
authors: Istituto di Chimica del Riconoscimento Molecolare (ICRM) - CNR c/o Università Cattolica del Sacro Cuore, Rome, Italy.
authors: Francesca
authors: F
authors: Sciandra
authors: Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy.
authors: Manuela
authors: M
authors: Bozzi
authors: Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy; Istituto di Chimica del Riconoscimento Molecolare (ICRM) - CNR c/o Università Cattolica del Sacro Cuore, Rome, Italy.
authors: Bruno
authors: B
authors: Giardina
authors: Istituto di Chimica del Riconoscimento Molecolare (ICRM) - CNR c/o Università Cattolica del Sacro Cuore, Rome, Italy.
authors: Andrea
authors: A
authors: Brancaccio
authors: Istituto di Chimica del Riconoscimento Molecolare (ICRM) - CNR c/o Università Cattolica del Sacro Cuore, Rome, Italy.
authors: Maria Cristina
authors: MC
authors: De Rosa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D049030:Dystroglycans / Q000737:chemistry* / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D006860:Hydrogen Bonding; D051379:Mice; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D055550:Protein Stability; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D029961:Zebrafish Proteins; D049030:Dystroglycans