title: Transplantation of pulmonary valve using a mouse model of heterotopic heart transplantation.
abstract: Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient's heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve.
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital; yongung.lee@nationwidechildrens.org.
authors: Yong-Ung
authors: YU
authors: Lee
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Tai
authors: T
authors: Yi
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Iyore
authors: I
authors: James
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Shuhei
authors: S
authors: Tara
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Alexander J
authors: AJ
authors: Stuber
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Kejal V
authors: KV
authors: Shah
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Avione Y
authors: AY
authors: Lee
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Tadahisa
authors: T
authors: Sugiura
authors: Cardiothoracic Surgery, Nationwide Children's Hospital.
authors: Narutoshi
authors: N
authors: Hibino
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital.
authors: Toshiharu
authors: T
authors: Shinoka
authors: Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital; Pediatric Surgery, Nationwide Children's Hospital.
authors: Christopher K
authors: CK
authors: Breuer
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D005260:Female; D016027:Heart Transplantation / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D023421:Models, Animal; D011664:Pulmonary Valve / Q000637:transplantation*; D020858:Tissue and Organ Harvesting / Q000379:methods; D016042:Transplantation, Heterotopic / Q000379:methods