title: On-demand optical immobilization of Caenorhabditis elegans for high-resolution imaging and microinjection.
abstract: This paper describes a novel selective immobilization technique based on optical control of the sol-gel transition of thermoreversible Pluronic gel, which provides a simple, versatile, and biocompatible approach for high-resolution imaging and microinjection of Caenorhabditis elegans.
authors: School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive NW, Atlanta, GA 30332-0100, USA. hang.lu@gatech.edu.
authors: Hyundoo
authors: H
authors: Hwang
authors: Jan
authors: J
authors: Krajniak
authors: Yohei
authors: Y
authors: Matsunaga
authors: Guy M
authors: GM
authors: Benian
authors: Hang
authors: H
authors: Lu
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D008845:Microinjections / Q000295:instrumentation* / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D012149:Restraint, Physical