title: Global regulator Anr represses PlcH phospholipase activity in Pseudomonas aeruginosa when oxygen is limiting.
abstract: Haemolytic phospholipase C (PlcH) is a potent virulence and colonization factor that is expressed at high levels by Pseudomonas aeruginosa within the mammalian host. The phosphorylcholine liberated from phosphatidylcholine and sphingomyelin by PlcH is further catabolized into molecules that both support growth and further induce plcH expression. We have shown previously that the catabolism of PlcH-released choline leads to increased activity of Anr, a global transcriptional regulator that promotes biofilm formation and virulence. Here, we demonstrated the presence of a negative feedback loop in which Anr repressed plcH transcription and we proposed that this regulation allowed for PlcH levels to be maintained in a way that promotes productive host-pathogen interactions. Evidence for Anr-mediated regulation of PlcH came from data showing that growth at low oxygen (1%) repressed PlcH abundance and plcH transcription in the WT, and that plcH transcription was enhanced in an Δanr mutant. The plcH promoter featured an Anr consensus sequence that was conserved across all P. aeruginosa genomes and mutation of conserved nucleotides within the Anr consensus sequence increased plcH expression under hypoxic conditions. The Anr-regulated transcription factor Dnr was not required for this effect. The loss of Anr was not sufficient to completely derepress plcH transcription as GbdR, a positive regulator of plcH, was required for expression. Overexpression of Anr was sufficient to repress plcH transcription even at 21 % oxygen. Anr repressed plcH expression and phospholipase C activity in a cell culture model for P. aeruginosa-epithelial cell interactions.
authors: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, 208 Vail Building, Hanover, NH 03755, USA.
authors: Angelyca A
authors: AA
authors: Jackson
authors: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, 208 Vail Building, Hanover, NH 03755, USA.
authors: Emily F
authors: EF
authors: Daniels
authors: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, 208 Vail Building, Hanover, NH 03755, USA.
authors: John H
authors: JH
authors: Hammond
authors: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, 208 Vail Building, Hanover, NH 03755, USA.
authors: Sven D
authors: SD
authors: Willger
authors: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, 208 Vail Building, Hanover, NH 03755, USA.
authors: Deborah A
authors: DA
authors: Hogan
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D004847:Epithelial Cells / Q000382:microbiology; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic; D017855:Transferases (Other Substituted Phosphate Groups) / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D015534:Trans-Activators; C072908:ANR protein, Pseudomonas aeruginosa; D017855:Transferases (Other Substituted Phosphate Groups); C477519:sphingomyelin synthase, Pseudomonas aeruginosa; D010100:Oxygen