title: Purification of high-molecular-weight subfraction from porcine skin inhibiting proliferation of A431 human carcinoma epidermoid cells.
abstract: Subfraction with a molecular weight >250 kDa isolated from porcine skin and inhibiting the proliferation of A431 human carcinoma epidermoid cells was purified by DEAE 32 anion exchange chromatography with NaCl concentration step-gradient. The effects of the initial subfraction and fractions obtained by separation in DEAE 32 on the proliferation of A431 human carcinoma epidermoid cells were studied in vitro in two tests (MTT and fluorescent test). The more sensitive fluorescent test showed the highest inhibitory activity of fraction No. 2 released from the column at 0.15 M NaCl. One major protein component and a series of minor protein components were detected in this fraction by vertical PAAG-SDS electrophoresis.
authors: Research Institute of Physico-Chemical Medicine, Federal Biomedical Agency of Russia, Moscow, Russia, olgabelova49@yandex.ru.
authors: O V
authors: OV
authors: Belova
authors: V I
authors: VI
authors: Sergienko
authors: V Ya
authors: VY
authors: Arion
authors: T A
authors: TA
authors: Lukanidina
authors: S N
authors: SN
authors: Moskvina
authors: I V
authors: IV
authors: Zimina
authors: G G
authors: GG
authors: Borisenko
authors: G V
authors: GV
authors: Lutsenko
authors: M V
authors: MV
authors: Grechikhina
authors: E V
authors: EV
authors: Kovaleva
authors: Zh I
authors: ZhI
authors: Klyuchnikova
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008970:Molecular Weight; D012867:Skin / Q000737:chemistry*; D034421:Sus scrofa; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D014020:Tissue Extracts