title: Wild chrysanthemum extract prevents UVB radiation-induced acute cell death and photoaging.
abstract: Wild chrysanthemum (Chrysanthemum indicum L.) is traditionally used in folk medicine as an anti-inflammatory agent. It is also used in the southwest plateau region of China to prevent ultraviolet-induced skin damage. However, the role and mechanism by which wild chrysanthemum prevents UV-induced skin damage and photoaging have never been investigated in vitro. In the present study, we found that aqueous extracts from wild chrysanthemum strongly reduced high-dose UVB-induced acute cell death of human immortalized keratinocytic HaCat cells. Wild chrysanthemum extract was also demonstrated to reduce low-dose UVB-induced expression of the photoaging-related matrix metalloproteinases MMP-2 and MMP-9. The ROS level elevated by UVB irradiation was strongly attenuated by wild chrysanthemum extract. Further study revealed that wild chrysanthemum extract reduced UVB-triggered ERK1/2 and p38 MAPK phosphorylation and their protective role, which is partially dependent on inhibiting p38 activation. These results suggest that wild chrysanthemum extract can protect the skin from UVB-induced acute skin damage and photoaging by reducing the intracellular reactive oxygen species (ROS) level and inhibiting p38 MAPK phosphorylation. The present study confirmed the protective role of wild chrysanthemum against UV-induced skin disorders in vitro and indicated the possible mechanism. Further study to identify the active components in wild chrysanthemum extract would be useful for developing new drugs for preventing and treating skin diseases, including skin cancer and photoaging, induced by UV irradiation.
authors: Department of Cosmetology, The Affiliated Hospital of Dali College, Dali, 671000, Yunnan, China.
authors: Sujiao
authors: S
authors: Sun
authors: Department of Pathology, Kunming Medical University, Kunming, 650032, Yunnan, China.
authors: Ping
authors: P
authors: Jiang
authors: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China.
authors: Weiting
authors: W
authors: Su
authors: Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, 650223, Yunnan, China. yang_xiang000@hotmail.com.
authors: Yang
authors: Y
authors: Xiang
authors: Kunming Biological Diversity Regional Center of Large Apparatuses and Equipment, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China.
authors: Jian
authors: J
authors: Li
authors: Kunming Biological Diversity Regional Center of Large Apparatuses and Equipment, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China.
authors: Lin
authors: L
authors: Zeng
authors: Kunming Biological Diversity Regional Center of Large Apparatuses and Equipment, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China.
authors: Shuangjuan
authors: S
authors: Yang
keywords: Anti-oxidant; Cell death; Chrysanthemum indicum L; Photoaging; Ultraviolet; Wild chrysanthemum