title: [VNTR-genotyping of Vibrio cholerae strains isolated from objects in the territory of Russian Federation in 2012].
abstract: OBJECTIVE
  VNTR-typing of Vibrio cholerae strains isolated in the territory of Russian Federation in 2012.
  
  
  METHODS
  71 Vibrio cholerae O3 and 3 V cholerae O1/O139 strains were used in the study. Genotyping was performed by using PCR for 5 VNTR-loci.
  
  
  RESULTS
  Multilocus VNTR-typing allowed to group the strains into 31 VNTR-genotypes. Genotypes were divided among 10 discrete clusters by results of a cluster analysis. The presence of tcpA gene is clearly linked with the presence of VcB locus. Each geographic region was characterized by their own VNTR-genotypes.
  
  
  CONCLUSIONS
  In the course of the carried out VNTR-genotyping of V. cholerae isolated in 2012, 2 types of vibrio population formation were detected. A geographic attachment to specific regions was characteristic for most of the genotypes.
authors: A S
authors: AS
authors: Vodop'ianov
authors: A B
authors: AB
authors: Mazrukho
authors: S O
authors: SO
authors: Vodop'ianov
authors: B N
authors: BN
authors: Mishan'kin
authors: V D
authors: VD
authors: Kruglikov
authors: I V
authors: IV
authors: Apkhangel'skaia
authors: I P
authors: IP
authors: Oleĭnikov
authors: D A
authors: DA
authors: Zubkova
authors: E V
authors: EV
authors: Monakhova
authors: L V
authors: LV
authors: Grigorenko
mesh_terms: D002771:Cholera / Q000453:epidemiology / Q000382:microbiology; D003470:Culture Media; D036781:Fimbriae Proteins / Q000145:classification / Q000235:genetics*; D015870:Gene Expression; D056426:Genetic Loci; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D058885:Multilocus Sequence Typing / Q000379:methods; D010802:Phylogeny; D058974:Phylogeography; D016133:Polymerase Chain Reaction; D012426:Russia / Q000453:epidemiology; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; C085807:TcpA protein, Vibrio cholerae; D036781:Fimbriae Proteins