title: Permianibacter aggregans gen. nov., sp. nov., a bacterium of the family Pseudomonadaceae capable of aggregating potential biofuel-producing microalgae.
abstract: A novel bacterial strain, capable of aggregating potential biofuel-producing microalgae, was isolated from the phycosphere of an algal culture and designated HW001(T). The novel bacterial strain was identified on the basis of its phylogenetic, genotypic, chemotaxonomic and phenotypic characteristics in this study. Cells were aerobic, Gram-negative rods. 16S rRNA gene-based phylogenetic analysis revealed that strain HW001(T) is affiliated with the family Pseudomonadaceae in the phylum Proteobacteria, but forms a distinct clade within this family. The DNA G+C content of strain HW001(T) was 55.4 mol%. The predominant cellular fatty acids were iso-C15:0, summed feature 9 (iso-C17:1ω9c), C16:0 and summed feature 3 (C16:1ω7c/C16:1ω6c). Q-8 was the main respiratory quinone. The polar lipid profile contained phosphatidylethanolamine, an unidentified aminophospholipid and some unidentified lipids. Based on the extensive polyphasic analysis, strain HW001(T) represents a novel species of a new genus in the family Pseudomonadaceae, for which the name Permianibacter aggregans gen. nov., sp. nov., is proposed. The type strain of the type species is HW001(T) ( = CICC 10856(T) = KCTC 32485(T)).
authors: Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD 21202, USA.|Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.|Yantai Institute of Costal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors: Hui
authors: H
authors: Wang
authors: Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors: Tianling
authors: T
authors: Zheng
authors: Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD 21202, USA.
authors: Russell T
authors: RT
authors: Hill
authors: Yantai Institute of Costal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors: Xiaoke
authors: X
authors: Hu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D056804:Biofuels; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D058086:Microalgae / Q000382:microbiology*; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D011548:Pseudomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D056804:Biofuels; D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C024223:vitamin MK 8