title: Cytokine expression of microscopic colitis including interleukin-17.
abstract: OBJECTIVE
  Microscopic colitis is characterized by chronic watery diarrhea with specific pathological changes that can be diagnosed by microscopic examination. We performed immunohistochemical analysis of proinflammatory cytokines to investigate the pathogenic mechanism of microscopic colitis.
  
  
  METHODS
  This study consisted of six patients with lymphocytic colitis, six patients with collagenous colitis, and six patients with functional diarrhea but normal pathology. We performed an immunohistochemical analysis of the colonic mucosal biopsies to assess the expression of cyclo-oxygenase-2, interleukin-17, nuclear factor-κB, interferon-γ, inducible nitric oxide synthase, and tumor necrosis factor-α. We compared the quantity score of immunohistochemical staining among the groups.
  
  
  RESULTS
  The microscopic colitis group showed significantly higher expression of cyclo-oxygenase-2, interleukin-17, nuclear factor-κB, and interferon-γ compared with the control group. Cytokine expression was similar between collagenous colitis and lymphocytic colitis. However, the expression of cyclo-oxygenase-2 was higher in collagenous colitis.
  
  
  CONCLUSIONS
  Proinflammatory cytokines, including interleukin-17 and interferon-γ, are highly expressed in microscopic colitis. The expression of cyclo-oxygenase-2 was higher in collagenous colitis than in lymphocytic colitis. This study is the first on interleukin-17 expression in microscopic colitis patients.
authors: Department of Internal Medicine, Eulji University School of Medicine, Korea.
authors: Eun-Kyoung
authors: EK
authors: Park
authors: Department of Internal Medicine, Eulji University School of Medicine, Korea.
authors: Young Sook
authors: YS
authors: Park
authors: Department of Internal Medicine, Eulji University School of Medicine, Korea.
authors: Dae Rim
authors: DR
authors: Park
authors: Department of Internal Medicine, Ewha University School of Medicine, Seoul, Korea.
authors: Sung Ae
authors: SA
authors: Jung
authors: Department of Internal Medicine, Hanyang University School of Medicine, Seoul, Korea.
authors: Dong Soo
authors: DS
authors: Han
authors: Department of Internal Medicine, Yeungnam University School of Medicine, Daegu, Department of Internal Medicine, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors: Byung Ik
authors: BI
authors: Jang
authors: Department of Internal Medicine, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors: Young Ho
authors: YH
authors: Kim
authors: Department of Internal Medicine, Yonsei University School of Medicine, Seoul, Korea.
authors: Won Ho
authors: WH
authors: Kim
authors: Department of Internal Medicine, Eulji University School of Medicine, Korea.
authors: Yun Ju
authors: YJ
authors: Jo
authors: Department of Biochemistry, Eulji University School of Medicine, Korea.
authors: Ki Ho
authors: KH
authors: Lee
authors: Department of Pathology, Eulji University School of Medicine, Korea.
authors: Won Mi
authors: WM
authors: Lee
authors: Department of Pathology, Eulji University School of Medicine, Korea.
authors: Eun Kyung
authors: EK
authors: Kim
authors: Department of Pathology, Ewha Womans University School of Medicine, Seoul, Korea.
authors: Hae Soo
authors: HS
authors: Koo
mesh_terms: D001706:Biopsy; D046728:Colitis, Microscopic / Q000378:metabolism*; D003106:Colon / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D003967:Diarrhea / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2
keywords: Colitis, collagenous; Colitis, lymphocytic; Colitis, microscopic; Immunohistochemistry