title: RIG-I knockdown impedes neurogenesis in a murine model of Japanese encephalitis.
abstract: Retinoic acid inducible gene I (RIG-I) is a well established pattern recognition receptor (PRR) in neurons infected with Japanese encephalitis virus (JEV) as reported previously from our laboratory. Japanese encephalitis (JE) virus infection in brain has been shown to decrease the proliferation of neural stem/progenitor cells (NSPCs) which has its implications in neurological sequelae in JE survivors. We have found that ablation of RIG-I both in vivo and in vitro models results in significant decrease in NSPC proliferation post JEV infection. We hypothesize that knockdown of RIG-I diminishes the expression of antiviral molecules resulting in an increase in viral replication, which in turn results in enhancement of the expression of cell cycle inhibitors, hence affecting the proliferation of NSPCs.
authors: National Brain Research Center, Manesar, Haryana, 122051, India.
authors: Sriparna
authors: S
authors: Mukherjee
authors: Sourish
authors: S
authors: Ghosh
authors: Arshed
authors: A
authors: Nazmi
authors: Anirban
authors: A
authors: Basu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004664:Encephalitis Virus, Japanese / Q000502:physiology; D004672:Encephalitis, Japanese / Q000473:pathology / Q000662:veterinary / Q000821:virology; D005260:Female; D008297:Male; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D011956:Receptors, Cell Surface; D014779:Virus Replication; D000097905:Roundabout Proteins
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; C118348:Robo3 protein, mouse; D000097905:Roundabout Proteins
keywords: Japanese encephalitis virus; knockdown; morpholino; neural stem/progenitor cells (NSPC); neurogenesis; retinoic acid inducible gene I (RIG-I)