title: Biomimetic alginate/polyacrylamide porous scaffold supports human mesenchymal stem cell proliferation and chondrogenesis.
abstract: We describe the development of alginate/polyacrylamide (ALG/PAAm) porous hydrogels based on interpenetrating polymer network structure for human mesenchymal stem cell proliferation and chondrogenesis. Three ALG/PAAm hydrogels at molar ratios of 10/90, 20/80, and 30/70 were prepared and characterized with enhanced elastic and rubbery mechanical properties, which are similar to native human cartilage tissues. Their elasticity and swelling properties were also studied under different physiological pH conditions. Finally, in vitro tests demonstrated that human mesenchymal stem cells could proliferate on the as-synthesized hydrogels with improved alkaline phosphatase activities. These results suggest that ALG/PAAm hydrogels may be a promising biomaterial for cartilage tissue engineering.
authors: Department of ENT-Head and Neck Surgery, EENT Hospital, Shanghai 200031, China; Shanghai Medical School, Fudan University, 210029, China.
authors: Peng
authors: P
authors: Guo
authors: Department of ENT-Head and Neck Surgery, EENT Hospital, Shanghai 200031, China; Shanghai Medical School, Fudan University, 210029, China; Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, United States. Electronic address: yuanyasheng@163.com.
authors: Yasheng
authors: Y
authors: Yuan
authors: Department of ENT-Head and Neck Surgery, EENT Hospital, Shanghai 200031, China; Shanghai Medical School, Fudan University, 210029, China.
authors: Fanglu
authors: F
authors: Chi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry* / Q000494:pharmacology; D000464:Alginates / Q000737:chemistry* / Q000494:pharmacology; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000187:drug effects*; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000464:Alginates; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D000099166:Polyacrylamides
keywords: Alginate; Chondrogenesis; Hydrogel; Mesenchymal stem cell; Polyacrylamide; Proliferation