title: Augmented cellular uptake of nanoparticles using tea catechins: effect of surface modification on nanoparticle-cell interaction.
abstract: Nanoparticles may serve as carriers in targeted therapeutics; interaction of the nanoparticles with a biological system may determine their targeting effects and therapeutic efficacy. Epigallocatechin-3-gallate (EGCG), a major component of tea catechins, has been conjugated with nanoparticles and tested as an anticancer agent. We investigated whether EGCG may enhance nanoparticle uptake by tumor cells. Cellular uptake of a dextran-coated magnetic nanoparticle (MNP) was determined by confocal microscopy, flow cytometry or a potassium thiocyanate colorimetric method. We demonstrated that EGCG greatly enhanced interaction and/or internalization of MNPs (with or without polyethylene glycol) by glioma cells, but not vascular endothelial cells. The enhancing effects are both time- and concentration-dependent. Such effects may be induced by a simple mix of MNPs with EGCG at a concentration as low as 1-3 μM, which increased MNP uptake 2- to 7-fold. In addition, application of magnetic force further potentiated MNP uptake, suggesting a synergetic effect of EGCG and magnetic force. Because the effects of EGCG were preserved at 4 °C, but not when EGCG was removed from the culture medium prior to addition of MNPs, a direct interaction of EGCG and MNPs was implicated. Use of an MNP-EGCG composite produced by adsorption of EGCG and magnetic separation also led to an enhanced uptake. The results reveal a novel interaction of a food component and nanocarrier system, which may be potentially amenable to magnetofection, cell labeling/tracing, and targeted therapeutics.
authors: Department of Physiology and Pharmacology, Institute of Biomedical Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kuei-Shan, Tao-Yuan 33302, Taiwan, Republic of China. yhma@mail.cgu.edu.tw.
authors: Yi-Ching
authors: YC
authors: Lu
authors: Pei-Chun
authors: PC
authors: Luo
authors: Chun-Wan
authors: CW
authors: Huang
authors: Yann-Lii
authors: YL
authors: Leu
authors: Tzu-Hao
authors: TH
authors: Wang
authors: Kuo-Chen
authors: KC
authors: Wei
authors: Hsin-Ell
authors: HE
authors: Wang
authors: Yunn-Hwa
authors: YH
authors: Ma
mesh_terms: D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D002460:Cell Line; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D004058:Diffusion; D005910:Glioma / Q000737:chemistry*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D010936:Plant Extracts; D002392:Catechin; C045651:epigallocatechin gallate