title: Transplanted human adipose tissue-derived stem cells engraft and induce regeneration in mice olfactory neuroepithelium in response to dichlobenil subministration.
abstract: We used immunodeficient mice, whose dorsomedial olfactory region was permanently damaged by dichlobenil inoculation, to test the neuroregenerative properties of transplanted human adipose tissue-derived stem cells after 30 and 60 days. Analysis of polymerase chain reaction bands revealed that stem cells preferentially engrafted in the lesioned olfactory epithelium compared with undamaged mucosa of untreated transplanted mice. Although basal cell proliferation in untransplanted lesioned mice did not give rise to neuronal cells in the olfactory mucosa, we observed clusters of differentiating olfactory cells in transplanted mice. After 30 days, and even more at 60 days, epithelial thickness was partially recovered to normal values, as also the immunohistochemical properties. Functional reactivity to odorant stimulation was also confirmed through electro-olfactogram recording in the dorsomedial epithelium. Furthermore, we demonstrated that engrafted stem cells fused with mouse cells in the olfactory organ, even if heterokaryons detected were too rare to hypothesize they directly repopulated the lesioned epithelium. The data reported prove that the migrating transplanted stem cells were able to induce a neuroregenerative process in a specific lesioned sensory area, enforcing the perspective that they could become an available tool for stem cell therapy.
authors: Department of Biological, Geological and Environmental Sciences, University of Bologna, and Foundation Onlus Stem Cells and Life, Via Selmi 3, 40126 Bologna, Italy, valeria.franceschini@unibo.it.
authors: Valeria
authors: V
authors: Franceschini
authors: Department of Biological, Geological and Environmental Sciences, University of Bologna, and Foundation Onlus Stem Cells and Life, Via Selmi 3, 40126 Bologna, Italy.
authors: Simone
authors: S
authors: Bettini
authors: International School for Advanced Studies, SISSA, Via Bonomea 265, 34136 Trieste, Italy.
authors: Simone
authors: S
authors: Pifferi
authors: International School for Advanced Studies, SISSA, Via Bonomea 265, 34136 Trieste, Italy.
authors: Anna
authors: A
authors: Menini
authors: Department of Clinical and Experimental Medicine, University of Pisa, Via Roma 67, 56126 Pisa, Italy.
authors: Gabriele
authors: G
authors: Siciliano
authors: IRCCS San Martino, National Institute for Cancer Research (IST), Largo Rosanna Benzi 10, 16132 Genua, Italy.
authors: Emanuela
authors: E
authors: Ognio
authors: Department of Biomedical, Surgical and Odontoiatric Sciences, University of Milan, Via Vanvitelli 32, 2019 Milan, Italy.
authors: Anna Teresa
authors: AT
authors: Brini
authors: Department of Hematology and Oncology "L. and A. Seragnoli," Section of Anatomic Pathology at Bellaria Hospital, University of Bologna, Via Altura 3, 40139 Bologna, Italy and.
authors: Enrico
authors: E
authors: Di Oto
authors: Institute for Chemical, Physical Processes, C.N.R. and Foundation Onlus Stem Cells and Life, Via L.L. Zamenhof 8, 56127 Pisa, Italy.
authors: Roberto P
authors: RP
authors: Revoltella
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000818:Animals; D005260:Female; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009416:Nerve Regeneration / Q000187:drug effects*; D046569:Neuroepithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009570:Nitriles / Q000008:administration & dosage / Q000494:pharmacology*; D009831:Olfactory Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
chemical_list: D009570:Nitriles; C055869:dichlobanil
keywords: Nod-scid mice; herbicide; olfactory mucosa; stem cell transplantation; tissue recovery