title: Selenium biofortification of broccoli and carrots grown in soil amended with Se-enriched hyperaccumulator Stanleya pinnata.
abstract: Amending soils with Se-hyperaccumulator plant derived sources of selenium (Se) may be useful for increasing the Se content in food crops in Se-deficient regions of the world. In this study we evaluated total Se and the different chemical species of Se in broccoli and carrots grown in soils amended with ground shoots of the Se-hyperaccumulator Stanleyapinnata. With increasing application rates of S. pinnata, total plant Se concentrations increased to nutritionally ideal levels inside edible parts. Selenium compounds in aqueous extracts were analyzed by SAX-HPLC-ICPMS and identified as a variety of mainly organic-Se forms. Together with bulk Se K-edge X-ray absorption near-edge structure (XANES) analysis performed on broccoli florets, carrot roots and shoots, dried ground S. pinnata, and the amended soil at post-plant, we demonstrate that Se-enriched S. pinnata is valuable as a soil amendment for enriching broccoli and carrots with healthful forms of organic-Se.
authors: USDA, Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Avenue, Parlier, CA 93648-9757, USA. Electronic address: gary.banuelos@ars.usda.gov.
authors: Gary S
authors: GS
authors: Bañuelos
authors: USDA, Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Avenue, Parlier, CA 93648-9757, USA.
authors: Irvin
authors: I
authors: Arroyo
authors: Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada.
authors: Ingrid J
authors: IJ
authors: Pickering
authors: Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada.
authors: Soo In
authors: SI
authors: Yang
authors: Department of Biology, California State University Fresno, Fresno, CA 93740, USA; Intrinsyx Technologies Corporation, Space Bio-Sciences Division, NASA-Ames Research Center, Moffett Field, CA 94035, USA. Electronic address: john.l.freeman@nasa.gov.
authors: John L
authors: JL
authors: Freeman
mesh_terms: D001937:Brassica / Q000737:chemistry*; D018552:Daucus carota / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D018036:Selenium Compounds / Q000032:analysis*; D012987:Soil / Q000737:chemistry*; D056928:X-Ray Absorption Spectroscopy
chemical_list: D018036:Selenium Compounds; D012987:Soil
keywords: Biofortification; Selenium; Stanleya pinnata