title: Specific amplification of gene encoding N-terminal region of catalase-peroxidase protein (KatG-N) for diagnosis of disseminated MAC disease in HIV patients.
abstract: Disseminated Mycobacterium avium-intracellulare complex (MAC) infection is considered as severe complication of advanced HIV/AIDS disease. Currently available various laboratory investigations have not only limited ability to discriminate between MAC infection and tuberculosis but are also laborious and time consuming. The aim of this study was, therefore, to design a molecular-based strategy for specific detection of MAC and its differentiation from Mycobacterium tuberculosis (M. tb) isolated from the blood specimens of HIV patients. A simple PCR was developed based on the amplification of 120-bp katG-N gene corresponding to the first 40 amino acids of N-terminal catalase-peroxidase (KatG) protein of Mycobacterium avium that shows only ~13% sequence homology by clustal W alignment to N-terminal region of M. tb KatG protein. This assay allowed the accurate and rapid detection of MAC bacteremia, distinguishing it from M. tb in a single PCR reaction without any need for sequencing or hybridization protocol to be performed thereafter. This study produced enough evidence that a significant proportion of Indian HIV patients have disseminated MAC bacteremia, suggesting the utility of M. avium katG-N gene PCR for early detection of MAC disease in HIV patients.
authors: Department of Biochemistry, PostGraduate Institute of Medical Education and Research, Sector-12, Chandigarh, India. Electronic address: romicalatawa@yahoo.co.in.
authors: Romica
authors: R
authors: Latawa
authors: Department of Pathology, New York University School of Medicine, New York University, NY, USA. Electronic address: kumark0006@gmail.com.
authors: Krishna Kumar
authors: KK
authors: Singh
authors: Department of Internal Medicine, PostGraduate Institute of Medical Education and Research, Sector-12, Chandigarh, India. Electronic address: awanchu@yahoo.com.
authors: Ajay
authors: A
authors: Wanchu
authors: Department of Medical Microbiology, PostGraduate Institute of Medical Education and Research, Sector-12, Chandigarh, India. Electronic address: sunilsethi10@hotmail.com.
authors: Sunil
authors: S
authors: Sethi
authors: Department of Medical Microbiology, PostGraduate Institute of Medical Education and Research, Sector-12, Chandigarh, India. Electronic address: sharmakusum9@yahoo.co.in.
authors: Kusum
authors: K
authors: Sharma
authors: Department of Internal Medicine, PostGraduate Institute of Medical Education and Research, Sector-12, Chandigarh, India. Electronic address: amansharma74@yahoo.com.
authors: Aman
authors: A
authors: Sharma
authors: Department of Pathology and Research Career Scientist, VA Medical Center, New York University, NY, USA. Electronic address: Suman.Laal@nyumc.org.
authors: Suman
authors: S
authors: Laal
authors: Department of Biochemistry, PostGraduate Institute of Medical Education and Research, Sector-12, Chandigarh, India. Electronic address: induvermabio@gmail.com.
authors: Indu
authors: I
authors: Verma
mesh_terms: D000328:Adult; D016470:Bacteremia / Q000175:diagnosis; D001426:Bacterial Proteins / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D015269:Mycobacterium avium Complex / Q000235:genetics / Q000302:isolation & purification*; D015270:Mycobacterium avium-intracellulare Infection / Q000175:diagnosis*; D010544:Peroxidases / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D010544:Peroxidases; C081610:catalase-peroxidase, bacteria
keywords: HIV; KatG; Mycobacterium avium-intracellulare complex; PCR