title: Lactobacillus acidophilus attenuates downregulation of DRA function and expression in inflammatory models.
abstract: Probiotics, including Lactobacilli, are commensal bacteria that have been used in clinical trials and experimental models for the prevention and treatment of diarrheal disorders. Our previous studies have shown that Lactobacillus acidophilus (LA) and its culture supernatant (CS) stimulated Cl(-)/HCO3 (-) exchange activity, acutely via an increase in the surface levels of downregulated in adenoma (DRA, SLC26A3) and in long-term treatments via increasing its expression involving transcriptional mechanisms. However, the role of LA in modulating DRA activity under inflammatory conditions is not known. Current in vitro studies using human intestinal epithelial Caco-2 cells examined the efficacy of LA or its CS in counteracting the inhibitory effects of interferon-γ (IFN-γ) on Cl(-)/HCO3 (-) exchange activity. Pretreatment of cells with LA or LA-CS for 1 h followed by coincubation with IFN-γ significantly alleviated the inhibitory effects of IFN-γ on Cl(-)/HCO3 (-) exchange activity. In the in vivo model of dextran sulfate sodium-induced experimental colitis (3% in drinking water for 7 days) in C57BL/6J mice, administration of live LA (3 × 10(9) colony-forming units) via oral gavage attenuated colonic inflammation. LA administration also counteracted the colitis-induced decrease in DRA mRNA and protein levels. Efficacy of LA or its secreted soluble factors in alleviating inflammation and inflammation-associated dysregulation of DRA activity could justify their therapeutic potential in inflammatory diarrheal diseases.
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and.
authors: Varsha
authors: V
authors: Singh
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and Singhania University, Pacheri Bari, Rajasthan, India.
authors: Anoop
authors: A
authors: Kumar
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and.
authors: Geetu
authors: G
authors: Raheja
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and.
authors: Arivarasu N
authors: AN
authors: Anbazhagan
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and.
authors: Shubha
authors: S
authors: Priyamvada
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and.
authors: Seema
authors: S
authors: Saksena
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and.
authors: Muhammad Nauman
authors: MN
authors: Jhandier
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and.
authors: Ravinder K
authors: RK
authors: Gill
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and.
authors: Waddah A
authors: WA
authors: Alrefai
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and.
authors: Alip
authors: A
authors: Borthakur
authors: Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois; and pkdudeja@uic.edu.
authors: Pradeep K
authors: PK
authors: Dudeja
mesh_terms: D000818:Animals; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D027963:Chloride-Bicarbonate Antiporters / Q000235:genetics / Q000378:metabolism*; D003092:Colitis / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control*; D003106:Colon / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D016264:Dextran Sulfate; D003967:Diarrhea / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control; D004195:Disease Models, Animal; D015536:Down-Regulation; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D007779:Lactobacillus acidophilus / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019936:Probiotics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D000076988:Sulfate Transporters; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection; D015431:Weight Loss
chemical_list: D017920:Antiporters; D027963:Chloride-Bicarbonate Antiporters; D018836:Inflammation Mediators; D012333:RNA, Messenger; C081011:SLC26A3 protein, human; C489860:Slc26a3 protein, mouse; D000076988:Sulfate Transporters; D007371:Interferon-gamma; D016264:Dextran Sulfate
keywords: Caco-2; dextran sulfate sodium; diarrhea; downregulated in adenoma; interferon-γ; probiotics