title: Effect of matrine on transforming growth factor β1 and hepatocyte growth factor in rat liver fibrosis model.
abstract: OBJECTIVE
  To observe the preventive and control effect of matrine on transforming growth factor (TGF-β1) and hepatocyte growth factor (HGF) of liver fibrosis tissue in rats.
  
  
  METHODS
  A total of 48 SD rats were randomly divided into A, B, C, D groups with 12 in each, group A as the normal control group and groups B, C, D as liver fibrosis models using composite modulus method with carbon tetrachloride (CCL4). Group B was the model group, group C adopted γ-interferon lavage therapy in the second day of modeling, and group D adopted matrine lavage treatment, at 4 and 8 weeks after treatment. Six rats were executed for detection of TGF-β1 and HGF, liver tissue histology and comparison fibrosis degree changes of rat liver tissue between groups.
  
  
  RESULTS
  Groups B, C, D showed a more significantly increased TGF-β1 at each time point compared with group A (P<0.05); Group B showed a more significantly increased TGF-β1 than groups C and D at weeks 4 and 8 (P<0.05); group D showed a lowest level of TGF-β1, followed by groups C and B. HGF of group B decreased more significantly than A group at weeks 4 and 8 (P<0.05); HGF of groups C and D was significantly elevated at 4 and 8 weeks than groups A and B (P<0.05), in which the group D showed the highest level of HGF. According to tissue histologic observation, rat liver tissue structure of group A was clear and normal, tissue structure of group B was destroyed with obvious fibrous tissue hyperplasia and fatty change of hepatic cells; groups C and D showed a slighter liver tissue damage, cell necrosis and connective tissue hyperplasia in collect abbacy than group B with a trend of obvious improvement.
  
  
  CONCLUSIONS
  Matrine can reduce TGF-β1 expression and enhance the activity of HGF, so as to realize the inhibition effect on liver fibrosis in rats.
authors: Heji Hospital affiliated to Changzhi Medical School, Changzhi 046011, China.
authors: Jian-Lan
authors: JL
authors: Yu
authors: Tumor Hospital of Shanxi Province, Taiyuan 030013, China.
authors: Jun-Hua
authors: JH
authors: Li
authors: Heji Hospital affiliated to Changzhi Medical School, Changzhi 046011, China.
authors: Rong-Gui
authors: RG
authors: Chengz
authors: Shanxi Medical School, Taiyuan 030001, China.
authors: Yan-Mei
authors: YM
authors: Ma
authors: Shanxi Medical School, Taiyuan 030001, China.
authors: Xiao-Juan
authors: XJ
authors: Wang
authors: The First Clinical Hospital of Shanxi Medical School, Taiyuan 030001, China. Electronic address: yjlantg@163.com.
authors: Jing-Chun
authors: JC
authors: Liu
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D015870:Gene Expression / Q000187:drug effects; D017228:Hepatocyte Growth Factor / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020011:Protective Agents / Q000494:pharmacology; D011807:Quinolizines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D020011:Protective Agents; D011807:Quinolizines; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D017228:Hepatocyte Growth Factor; D000093842:Matrines
keywords: Hepatocyte growth factor; Liver fibrosis; Matrine; Transforming growth factor β1