title: Development trends for generation of single-chain antibody fragments.
abstract: Recombinant antibodies are increasingly being employed as therapeutic agents especially in combination with anti-cancer drugs. The single-chain antibody fragments are small antigen-binding proteins which provide the most commonly used antibody formats for diagnostic and therapeutic purposes. These antibody fragments have more rapid tumor penetration and clearance from the serum relative to full-length monoclonal antibodies. There are in vitro antibody-display technologies such as phage display, cell surface display, ribosome display and mRNA display that can be used to isolate high specificity and affinity single-chain antibodies against a wide variety of targets. We review these strategies for generation of stable and active antibody fragments in the present article.
authors: Biotechnology Research Center .
authors: Safar
authors: S
authors: Farajnia
authors: Vahideh
authors: V
authors: Ahmadzadeh
authors: Asghar
authors: A
authors: Tanomand
authors: Kamal
authors: K
authors: Veisi
authors: Shiva Ahdi
authors: SA
authors: Khosroshahi
authors: Leila
authors: L
authors: Rahbarnia
mesh_terms: D000818:Animals; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000276:immunology*; D007155:Immunologic Factors / Q000737:chemistry / Q000276:immunology*; D019151:Peptide Library; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000276:immunology*; D014780:Viruses / Q000276:immunology
chemical_list: D007128:Immunoglobulin Fragments; D007155:Immunologic Factors; D019151:Peptide Library; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies
keywords: Display methods; improved stability; single-chain antibody fragments