title: Antibody preparation and identification of the Cashmere goat c-kit protein in the testes.
abstract: The c-kit protein plays a major role in the regulation of germ cell development. Its expression and distribution in rodent testes have been widely reported. However, research regarding c-kit expression in domestic animals is scarce, and the expression pattern and distribution of c-kit in germ cells have not been clearly defined. In this study, a specific antigenic region for goat c-kit was designed, and a c-kit polyclonal antibody was prepared. This antibody was then applied in a study evaluating c-kit expression in Cashmere goat tissues. A Western blot analysis showed that three forms of c-kit were expressed in goat testes: precursor, mature, and soluble c-kit. Fluorescent immunohistochemical analyses showed that c-kit was primarily expressed in the spermatogonia and spermatocytes of goat testes. These results not only clarify the expression and localization of c-kit in the goat testis, but also accelerate further research regarding the function of c-kit in goat spermatogenesis.
authors: Key Laboratory of China Education Ministry for the Research of Mammal Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China.
authors: S C L
authors: SC
authors: Wu
authors: Key Laboratory of China Education Ministry for the Research of Mammal Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China.
authors: F H
authors: FH
authors: Luo
authors: Key Laboratory of China Education Ministry for the Research of Mammal Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China.
authors: Q F
authors: QF
authors: Kong
authors: Key Laboratory of China Education Ministry for the Research of Mammal Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, China yingji_wu@yahoo.com.
authors: Y J
authors: YJ
authors: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000302:isolation & purification*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006041:Goats / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017433:Protein Structure, Secondary; D019009:Proto-Oncogene Proteins c-kit / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism*; D013091:Spermatogenesis / Q000235:genetics; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D011498:Protein Precursors; D011994:Recombinant Proteins; D019009:Proto-Oncogene Proteins c-kit