title: Magnetic resonance imaging of melanoma exosomes in lymph nodes.
abstract: OBJECTIVE
  Exosomes are cell derived extracellular nanovesicles that relay molecular signals pertinent to both normal physiologic and disease processes. The ability to modify and track exosomes in vivo is essential to understanding exosome pathogenesis, and for utilizing exosomes as effective diagnostic and therapeutic nanocarriers to treat diseases.
  
  
  METHODS
  We recently reported a new electroporation method that allow exosomes to be loaded with superparamagnetic iron oxide nanoparticles for magnetic resonance tracking.
  
  
  RESULTS
  Building on this approach, we now demonstrate for the first time using a C57BL/6 mouse model that melanoma exosomes can be imaged in vitro, and within lymph nodes in vivo with the use of standard MRI approaches.
  
  
  CONCLUSIONS
  These findings demonstrate proof of principle that exosome biology can be followed in vivo and pave the way for the development of future diagnostic and therapeutic applications. Magn Reson Med 74:266-271, 2015. © 2014 Wiley Periodicals, Inc.
authors: Consortium for Translational Research in Advanced Imaging and Nanomedicine (C-TRAIN), Washington University School of Medicine, St. Louis, Missouri, USA.
authors: Lingzhi
authors: L
authors: Hu
authors: Consortium for Translational Research in Advanced Imaging and Nanomedicine (C-TRAIN), Washington University School of Medicine, St. Louis, Missouri, USA.
authors: Samuel A
authors: SA
authors: Wickline
authors: Consortium for Translational Research in Advanced Imaging and Nanomedicine (C-TRAIN), Washington University School of Medicine, St. Louis, Missouri, USA.
authors: Joshua L
authors: JL
authors: Hood
keywords: exosome; lymph node; magnetic resonance imaging; melanoma; nanomedicine; superparamagnetic iron oxide