title: Ebb-and-flow of macroautophagy and chaperone-mediated autophagy in Raji cells induced by starvation and arsenic trioxide.
abstract: Autophagy is crucial in the maintenance of homeostasis and regenerated energy of mammalian cells. Macroautophagy and chaperone-mediated autophagy(CMA) are the two best-identified pathways. Recent research has found that in normal cells, decline of macroautophagy is appropriately parallel with activation of CMA. However, whether it is also true in cancer cells has been poorly studied. Here we focused on cross-talk and conversion between macroautophagy and CMA in cultured Burkitt lymphoma Raji cells when facing serum deprivation and exposure to a toxic compound, arsenic trioxide. The results showed that both macroautophagy and CMA were activated sequentially instead of simultaneously in starvation-induced Raji cells, and macroautophagy was quickly activated and peaked during the first hours of nutrition deprivation, and then gradually decreased to near baseline. With nutrient deprivation persisted, CMA progressively increased along with the decline of macroautophagy. On the other hand, in arsenic trioxide-treated Raji cells, macroautophagy activity was also significantly increased, but CMA activity was not rapidly enhanced until macroautophagy was inhibited by 3-methyladenine, an inhibitor. Together, we conclude that cancer cells exhibit differential responses to diverse stressor-induced damage by autophagy. The sequential switch of the first-aider macroautophagy to the homeostasis-stabilizer CMA, whether active or passive, might be conducive to the adaption of cancer cells to miscellaneous intracellular or extracellular stressors. These findings must be helpful to understand the characteristics, compensatory mechanisms and answer modes of different autophagic pathways in cancer cells, which might be very important and promising to the development of potential targeting interventions for cancer therapies via regulation of autophagic pathways.
authors: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, China E-mail : weihulai@lzu.edu.cn.
authors: Cai-Li
authors: CL
authors: Li
authors: Hu-Lai
authors: HL
authors: Wei
authors: Jing
authors: J
authors: Chen
authors: Bei
authors: B
authors: Wang
authors: Bei
authors: B
authors: Xie
authors: Lin-Lan
authors: LL
authors: Fan
authors: Lin-Jing
authors: LJ
authors: Li
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D001343:Autophagy / Q000187:drug effects*; D002051:Burkitt Lymphoma / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D006801:Humans; D018832:Molecular Chaperones / Q000378:metabolism*; D010087:Oxides / Q000494:pharmacology*; D013217:Starvation
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D018832:Molecular Chaperones; D010087:Oxides; C025946:3-methyladenine; D000225:Adenine; D000077237:Arsenic Trioxide