title: Characterization of arsenic-resistant endophytic bacteria from hyperaccumulators Pteris vittata and Pteris multifida.
abstract: We isolated and characterized As-resistant endophytic bacteria (AEB) from two arsenic hyperaccumulators. Their plant growth promoting traits and the relation between As tolerance and transformation were evaluated. A total of 41 and 33 AEB were isolated from Pteris vittata (PV) and Pteris multifida (PM) respectively. PV AEB represented 2genera while PM AEB comprised of 12 genera, with Bacillus sp. being the most dominant bacteria from both plants. All AEB had limited ability in solubilizing P and producing indole acetic acid (IAA) and siderophore. All isolates tolerated 10mM arsenate (As(V)), with PV isolates being more tolerant to As(V) and PM more tolerant to arsenite (As(III)). Bacterial arsenic tolerance was related to their ability in As(III) oxidation and As(V) reduction as well as their ability to retain As in the biomass to a varying extent. Though AEB showed limited plant growth promoting traits, they were important in arsenic tolerance and speciation in plants.
authors: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China.
authors: Ling-Jia
authors: LJ
authors: Zhu
authors: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China.
authors: Dong-Xing
authors: DX
authors: Guan
authors: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China.
authors: Jun
authors: J
authors: Luo
authors: Horticultural Sciences, University of Florida, Gainesville, FL 32611, United States.
authors: Bala
authors: B
authors: Rathinasabapathi
authors: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210023, China; Soil and Water Science Department, University of Florida, Gainesville, FL 32611, United States. Electronic address: lqma@ufl.edu.
authors: Lena Q
authors: LQ
authors: Ma
mesh_terms: D000704:Analysis of Variance; D001149:Arsenates; D001151:Arsenic / Q000378:metabolism*; D018053:Arsenites; D001407:Bacillus / Q000235:genetics / Q000502:physiology*; D001673:Biodegradation, Environmental; D017931:DNA Primers / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D060026:Endophytes / Q000235:genetics / Q000502:physiology*; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D032495:Pteris / Q000378:metabolism / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D001149:Arsenates; D018053:Arsenites; D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid
keywords: Endophytic bacteria; Pteris multifida; Pteris vittata; Tolerance; Transformation