title: Generation of transgenic mouse fluorescent reporter lines for studying hematopoietic development.
abstract: During the development of the hematopoietic system, at least eight distinct lineages are generated in the mouse embryo. Transgenic mice expressing fluorescent proteins at various points in the hematopoietic hierarchy, from hematopoietic stem cell to multipotent progenitors to each of the final differentiated cell types, have provided valuable tools for tagging, tracking, and isolating these cells. In this chapter, we discuss general considerations in designing a transgene and survey available fluorescent probes and methods for confirming and analyzing transgene expression in the hematopoietic systems of the embryo, fetus, and postnatal/adult animal.
authors: Department of Medicine, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, 1079, New York, NY, 10029-6574, USA.
authors: Andrei M
authors: AM
authors: Vacaru
authors: Joseph
authors: J
authors: Vitale
authors: Johnathan
authors: J
authors: Nieves
authors: Margaret H
authors: MH
authors: Baron
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004210:Dissection; D004622:Embryo, Mammalian / Q000378:metabolism; D005434:Flow Cytometry; D005453:Fluorescence; D017930:Genes, Reporter / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D006410:Hematopoiesis; D008164:Luminescent Proteins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D008853:Microscopy
chemical_list: D008164:Luminescent Proteins; D004247:DNA