title: Improved beta-carotene and lycopene production by Blakeslea trispora with ultrasonic treatment in submerged fermentation.
abstract: Response surface methodology (RSM) based on Box-Behnken design (BBD) was employed to investigate the effect of ultrasonic treatment on beta-carotene production by Blakeslea trispora. The optimized strategy involved exposing three-day-old mycelial cultures to ultrasonic treatment at a fixed frequency of 20 kHz, power of 491 W, treatment time of 3 min, working time of 3 s, and rest time of 5.8 s, repeated four times at a 24-h interval. Mycelium growth was not significantly promoted under ultrasonic stimulation; however, the glucose metabolism increased by about 10%, the average size of the aggregates significantly decreased, and the uptake rate of imidazole into cells was increased about 2.5-fold. After a 6-d culture, the technique produced 173 mg/L of beta-carotene and 82 mg/L of lycopene, which represented an increase of nearly 40.7% and 52.7%, respectively, over the yields obtained in cultures without ultrasonic treatment.
authors: Hong-Bo
authors: HB
authors: Wang
authors: Rong-Gang
authors: RG
authors: Xu
authors: Long-Jiang
authors: LJ
authors: Yu
authors: Jun
authors: J
authors: Luo
authors: Li-Wei
authors: LW
authors: Zhang
authors: Xiao-Yan
authors: XY
authors: Huang
authors: Wen-An
authors: WA
authors: Zou
authors: Qian
authors: Q
authors: Zhao
authors: Ming-Bo
authors: MB
authors: Lu
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D007093:Imidazoles / Q000032:analysis; D000077276:Lycopene; D009090:Mucorales / Q000254:growth & development / Q000378:metabolism*; D025282:Mycelium / Q000254:growth & development / Q000378:metabolism; D014465:Ultrasonics; D019207:beta Carotene / Q000096:biosynthesis*
chemical_list: D007093:Imidazoles; D019207:beta Carotene; D002338:Carotenoids; D000077276:Lycopene