title: Royal Jelly-Mediated Prolongevity and Stress Resistance in Caenorhabditis elegans Is Possibly Modulated by the Interplays of DAF-16, SIR-2.1, HCF-1, and 14-3-3 Proteins.
abstract: Recent studies suggest that royal jelly (RJ) and its related substances may have antiaging properties. However, the molecular mechanisms underlying the beneficial effects remain elusive. We report that the effects of RJ and enzyme-treated RJ (eRJ) on life span and health span in Caenorhabditis elegans (C elegans) are modulated by the sophisticated interplays of DAF-16, SIR-2.1, HCF-1, and 14-3-3 proteins. Dietary supplementation with RJ or eRJ increased C. elegans life span in a dose-dependent manner. The RJ and eRJ consumption increased the tolerance of C elegans to oxidative stress, ultraviolet irradiation, and heat shock stress. Our genetic analyses showed that RJ/eRJ-mediated life-span extension requires insulin/IGF-1 signaling and the activities of DAF-16, SIR-2.1, HCF-1, and FTT-2, a 14-3-3 protein. Earlier studies reported that DAF-16/FOXO, SIR-2.1/SIRT1, FTT-2, and HCF-1 have extensive interplays in worms and mammals. Our present findings suggest that RJ/eRJ-mediated promotion of longevity and stress resistance in C elegans is dependent on these conserved interplays. From an evolutionary point of view, this study not only provides new insights into the molecular mechanisms of RJ's action on health span promotion in C elegans, but also has imperative implications in using RJ/eRJ as nutraceuticals to delay aging and age-related disorders.
authors: Department of Biological Sciences and.
authors: Xiaoxia
authors: X
authors: Wang
authors: Department of Biological Sciences and.
authors: Lauren F
authors: LF
authors: Cook
authors: Department of Biological Sciences and.
authors: Lindsay M
authors: LM
authors: Grasso
authors: Department of Biological Sciences and Institute for Engaged Aging, Clemson University, South Carolina.
authors: Min
authors: M
authors: Cao
authors: Department of Biological Sciences and Institute for Engaged Aging, Clemson University, South Carolina. ydong@clemson.edu.
authors: Yuqing
authors: Y
authors: Dong
mesh_terms: D048948:14-3-3 Proteins / Q000502:physiology*; D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000502:physiology*; D005227:Fatty Acids / Q000494:pharmacology*; D051858:Forkhead Transcription Factors / Q000502:physiology*; D051863:Host Cell Factor C1 / Q000502:physiology*; D008136:Longevity / Q000187:drug effects*; D037761:Sirtuins / Q000502:physiology*; D013312:Stress, Physiological / Q000187:drug effects; D000099219:Royal Jelly
chemical_list: D048948:14-3-3 Proteins; D029742:Caenorhabditis elegans Proteins; D005227:Fatty Acids; D051858:Forkhead Transcription Factors; D051863:Host Cell Factor C1; D037761:Sirtuins; D000099219:Royal Jelly; C108831:daf-16 protein, C elegans; C497691:hcf-1 protein, C elegans; C507014:SIR-2.1 protein, C elegans
keywords: Aging intervention; Caenorhabditis elegans.; DAF-16; Gene expression; Longevity; Royal jelly