title: Preparation of acellular myocardial scaffolds with well-preserved cardiomyocyte lacunae, and method for applying mechanical and electrical simulation to tissue construct.
abstract: Cardiac tissue engineering/regeneration using decellularized myocardium has attracted great research attention due to its potential benefit for myocardial infarction (MI) treatment. Here we describe an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulation for facilitating cardiac construct development.
authors: Tissue Bioengineering Laboratory, Department of Biological Engineering, Mississippi State University, 130 Creelman Street, Room 222, Mississippi State, MS, 39762, USA.
authors: Bo
authors: B
authors: Wang
authors: Lakiesha N
authors: LN
authors: Williams
authors: Amy L
authors: AL
authors: de Jongh Curry
authors: Jun
authors: J
authors: Liao
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D004558:Electric Stimulation; D005109:Extracellular Matrix; D055595:Mechanical Phenomena; D032383:Myocytes, Cardiac / Q000166:cytology*; D011309:Preservation, Biological; D013242:Sterilization; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds