title: Genetically engineered insertional mutagenesis in mice to model cancer: Sleeping Beauty.
abstract: The ability to accurately model human cancer in mice enables in vivo examination of the biological mechanisms related to cancer initiation and progression as well as preclinical testing of new anticancer treatments and potential targets. The emergence of the genetically engineered Sleeping Beauty system of insertional mutagenesis has led to the development of a new generation of genetic mouse models of cancer and identification of novel cancer-causing genes. This chapter reviews the published cancer models of Sleeping Beauty and strategies using available strains to generate several models of cancer.
authors: Bill Walsh Translational Cancer Research Laboratory, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, Level 8, Kolling Building, St Leonards, NSW, 2065, Australia, viive.howell@sydney.edu.au.
authors: Viive M
authors: VM
authors: Howell
authors: Emily K
authors: EK
authors: Colvin
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001947:Breeding; D004251:DNA Transposable Elements / Q000235:genetics*; D004195:Disease Models, Animal; D006801:Humans; D019426:Integrases / Q000378:metabolism; D008297:Male; D051379:Mice; D016254:Mutagenesis, Insertional / Q000379:methods*; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D019076:Transgenes / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; C045073:Cre recombinase; D019426:Integrases