title: [Effect of rosiglitazone on the expression of HIF1α and IGF1 mRNA in myeloma].
abstract: OBJECTIVE
  To observe the effect of rosiglitazone (RGZ) on the mRNA expression of HIF1α and IGF1 genes in the myeloma cells and explore possible mechanism of angiogenesis inhibition.
  
  
  METHODS
  Human myeloma cell line RPMI8226 and primary myeloma cells from five patients enriched by using CD138 immunomagnetic beads were treated with different concentrations (10, 20, 40 μmol/L) of RGZ. The mRNA expression of HIF1α and IGF1 was analyzed in cells treated with RGZ after 48h by RT-PCR, The levels of phosphorylated AKT and ERK proteins were detected by Western blotting. Bone marrow mononuclear cells from five patients with iron deficiency anemia were regarded as control.
  
  
  RESULTS
  Higher mRNA expression of HIF1α and IGF1 genes in RPMI8226 and in primary myeloma cells was showed as compared to those in control. Treated with RGZ of 20 μmol/L after 48 h, the mRNA expression of HIF1α (1.21 ± 0.08 vs 0.75 ± 0.06) and IGF1 (0.62 ± 0.06 vs 0.32 ± 0.04) in RPMI8226 cells was declined as compared to those without RGC treatment. The same declination was also seen in primary myeloma cells (HIF1α: 2.02 ± 0.16 vs 0.53 ± 0.04; IGF1: 1.92 ± 0.13 vs 0.58±0.03). RGZ could inhibit the expression of pAKT and pERK, nor the total AKT and ERK proteins, in RPMI8226 cells in a dose-dependent manner at the concentration of 10 μmol/L, 20 μmol/L, and 40 μmol/L.
  
  
  CONCLUSIONS
  RGZ could inhibit the mRNA expression of HIF1α and IGF1. Inhibition of angiogenesis by RGZ may be associated with down-regulation of pAKT and pERK expression.
authors: Department of Hematology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Mingzhong
authors: M
authors: Rui
authors: Department of Hematology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Tianwen
authors: T
authors: Fu
authors: Department of Hematology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Ying
authors: Y
authors: Liu
authors: Department of Hematology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Haiwen
authors: H
authors: Huang
authors: Department of Hematology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Jinxiang
authors: J
authors: Fu
mesh_terms: D045744:Cell Line, Tumor; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D009101:Multiple Myeloma / Q000098:blood supply / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology*
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C583557:IGF1 protein, human; D012333:RNA, Messenger; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D007334:Insulin-Like Growth Factor I; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases