title: Role of the C-type lectin receptors MCL and DCIR in experimental colitis.
abstract: Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract. Though its exact etiology is still unclear, it is proposed that an imbalance in the intestinal homeostasis leads to a disturbed interaction between commensal microbiota and the mucosal immune system. Previous studies have shown that both innate and adaptive immunity are involved in an overwhelming colon inflammation, and thus contribute to the pathogenesis of IBD. In innate immunity, several pattern recognition receptors such as Toll-like receptors, NOD-like receptors or C-type lectin receptors (CLRs) are involved in IBD pathogenesis. Myeloid CLRs are mainly expressed by antigen-presenting cells and bind to glycan structures present on self or foreign antigens. The Macrophage-restricted C-type lectin (MCL) and the Dendritic cell immunoreceptor (DCIR) are two poorly characterized members of the CLR family. In this study, we investigated the role of MCL and DCIR in the pathogenesis of murine colitis. Both CLRs bound to intestinal microbiota to a different extent. They modulated the production of pro-inflammatory cytokines by antigen-presenting cells upon stimulation with heat-killed microbiota and impacted subsequent T cell responses. To analyze whether MCL and DCIR contribute to the pathogenesis of IBD, the dextran sulfate sodium (DSS) murine colitis model was employed. MCL-/- as well as DCIR-/- mice exhibited only a slightly increased severity of disease compared to wild-type mice indicating a limited role for MCL and DCIR in the regulation of intestinal immunity.
authors: Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Potsdam, Germany; Freie Universität Berlin, Institute of Chemistry and Biochemistry, Department of Biology, Chemistry and Pharmacy, Berlin, Germany.
authors: Julia
authors: J
authors: Hütter
authors: Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Potsdam, Germany; Freie Universität Berlin, Institute of Chemistry and Biochemistry, Department of Biology, Chemistry and Pharmacy, Berlin, Germany.
authors: Magdalena
authors: M
authors: Eriksson
authors: Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Potsdam, Germany; Freie Universität Berlin, Institute of Chemistry and Biochemistry, Department of Biology, Chemistry and Pharmacy, Berlin, Germany.
authors: Timo
authors: T
authors: Johannssen
authors: Freie Universität Berlin, Department of Veterinary Pathology, Berlin, Germany.
authors: Robert
authors: R
authors: Klopfleisch
authors: Freie Universität Berlin, Department of Veterinary Pathology, Berlin, Germany.
authors: Dorthe
authors: D
authors: von Smolinski
authors: Freie Universität Berlin, Department of Veterinary Pathology, Berlin, Germany.
authors: Achim D
authors: AD
authors: Gruber
authors: Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Potsdam, Germany; Freie Universität Berlin, Institute of Chemistry and Biochemistry, Department of Biology, Chemistry and Pharmacy, Berlin, Germany.
authors: Peter H
authors: PH
authors: Seeberger
authors: Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Potsdam, Germany; Freie Universität Berlin, Institute of Chemistry and Biochemistry, Department of Biology, Chemistry and Pharmacy, Berlin, Germany.
authors: Bernd
authors: B
authors: Lepenies
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D016264:Dextran Sulfate / Q000009:adverse effects; D004195:Disease Models, Animal; D015870:Gene Expression; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D064307:Microbiota; D011485:Protein Binding; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; C526731:Dcir protein, mouse; D037181:Lectins, C-Type; D016264:Dextran Sulfate