title: Genetic linkage map construction and QTL identification of juvenile growth traits in Torreya grandis.
abstract: Torreya grandis Fort. ex Lindl, a conifer species widely distributed in Southeastern China, is of high economic value by producing edible, nutrient seeds. However, knowledge about the genome structure and organization of this species is poorly understood, thereby limiting the effective use of its gene resources. Here, we report on a first genetic linkage map for Torreya grandis using 96 progeny randomly chosen from a half-sib family of a commercially cultivated variety of this species, Torreya grandis Fort. ex Lindl cv. Merrillii. The map contains 262 molecular markers, i.e., 75 random amplified polymorphic DNAs (RAPD), 119 inter-simple sequence repeats (ISSR) and 62 amplified fragments length polymorphisms (AFLP), and spans a total of 7,139.9 cM, separated by 10 linkage groups. The linkage map was used to map quantitative trait loci (QTLs) associated with juvenile growth traits by functional mapping. We identified four basal diameter-related QTLs on linkage groups 1, 5 and 9; four height-related QTLs on linkage groups 1, 2, 5 and 8. It was observed that the genetic effects of QTLs on growth traits vary with age, suggesting the dynamic behavior of growth QTLs. Part of the QTLs was found to display a pleiotropic effect on basal diameter growth and height growth.
authors: Yanru
authors: Y
authors: Zeng
authors: Shengyue
authors: S
authors: Ye
authors: Weiwu
authors: W
authors: Yu
authors: Song
authors: S
authors: Wu
authors: Wei
authors: W
authors: Hou
authors: Rongling
authors: R
authors: Wu
authors: Wensheng
authors: W
authors: Dai
authors: Jun
authors: J
authors: Chang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D002681:China; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D008040:Genetic Linkage; D018895:Microsatellite Repeats; D040641:Quantitative Trait Loci; D019105:Random Amplified Polymorphic DNA Technique; D032490:Taxaceae / Q000235:genetics* / Q000254:growth & development
chemical_list: D018744:DNA, Plant