title: Simultaneous determination of atorvastatin calcium and olmesartan medoxomil in a pharmaceutical formulation by reversed phase high-performance liquid chromatography, high-performance thin-layer chromatography, and UV spectrophotometric methods.
abstract: Validated RP-HPLC, HPTLC, and UV spectrophotometric methods have been developed for the simultaneous determination of atorvastatin calcium (ATV) and olmesartan medoxomil (OLM) in a pharmaceutical formulation. The RP-HPLC separation was achieved on a Kromasil C18 column (250 x 4.6 mm, 5 microm particle size) using 0.01 M potassium dihydrogen o-phosphate (pH 4 adjusted with o-phosphoric acid)-acetonitrile (50 + 50, v/v) as the mobile phase at a flow rate of 1.5 mL/min. Quantification was achieved by UV detection at 276 nm. The HPTLC separation was achieved on precoated silica gel 60F254 plates using chloroform-methanol-acetonitrile (4 + 2+ 4, v/v/v) mobile phase. Quantification was achieved with UV detection at 276 nm. The UV-Vis spectrophotometric method was based on the simultaneous equation method that involves measurement of absorbance at two wavelengths, i.e., 255 nm (lambda max of OLM) and 246.2 nm (lambda max of ATV) in methanol. All three methods were validated as per International Conference on Harmonization guidelines. The proposed methods were simple, precise, accurate, and applicable for the simultaneous determination of ATV and OLM in a marketed formulation. The results obtained by applying the proposed methods were statistically analyzed and were found satisfactory.
authors: Hiral
authors: H
authors: Soni
authors: Charmy
authors: C
authors: Kothari
authors: Deepak
authors: D
authors: Khatri
authors: Priti
authors: P
authors: Mehta
mesh_terms: D000069059:Atorvastatin; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D006538:Heptanoic Acids / Q000032:analysis*; D007093:Imidazoles / Q000032:analysis*; D000068557:Olmesartan Medoxomil; D011758:Pyrroles / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013777:Tetrazoles / Q000032:analysis*
chemical_list: D006538:Heptanoic Acids; D007093:Imidazoles; D011758:Pyrroles; D013777:Tetrazoles; D000068557:Olmesartan Medoxomil; D000069059:Atorvastatin