title: Investigation of nonfouling polypeptides of poly(glutamic acid) with lysine side chains synthesized by EDC·HCl/HOBt chemistry.
abstract: Nonfouling polypeptides with homogenous alternating charges draw peoples' attentions for their potential capability in biodegradation. Homogenous glutamic acid (E) and lysine (K) polypeptides were proposed and synthesized before. In this work, a new polypeptide formed by poly(glutamic acid) with lysine side chains (poly(E)-K) was synthesized by facile EDC·HCl/HOBt chemistry and investigated. Results show that these polypeptides also have good nonspecific protein resistance determined by enzyme-linked immunosorbent assay. The lowest nonspecific adsorption of the model proteins, anti-IgG and fibrinogen (Fg), on the self-assembling monolayers (SAMs) surface of poly(E)-K was only 3.3 ± 1.8 and 4.4 ± 1.6%, respectively, when protein adsorption on tissue culture polystyrene surface was set as 100%. And, the relative nonspecific protein adsorption increases when the polypeptide molecular weight increases due to the repression of low density polymer brushes. Moreover, almost no obvious cytotoxicity and hemolytic activity in vitro were detected. This work suggests that polypeptides with various formats of homogenous balanced charges could achieve excellent nonspecific protein resistance, which might be the intrinsic reason for the coexistence of high concentration serum proteins in blood.
authors: a Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering , Zhejiang University , Hangzhou 310027 , P.R. China.
authors: Qinghua
authors: Q
authors: Yang
authors: Wenchen
authors: W
authors: Li
authors: Longgang
authors: L
authors: Wang
authors: Guangzhi
authors: G
authors: Wang
authors: Zhen
authors: Z
authors: Wang
authors: Lingyun
authors: L
authors: Liu
authors: Shengfu
authors: S
authors: Chen
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D057219:Biofouling / Q000517:prevention & control*; D002470:Cell Survival / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D008239:Lysine / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D014230:Triazoles / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D007074:Immunoglobulin G; D010455:Peptides; D014230:Triazoles; D018698:Glutamic Acid; D005340:Fibrinogen; D008239:Lysine; D006851:Hydrochloric Acid; D005022:Ethyldimethylaminopropyl Carbodiimide
keywords: SAMs; biocompatibility; nonfouling; poly(E)-K; polypeptides