title: Using crude whole-genome assemblies of Neisseria gonorrhoeae as a platform for strain analysis: clonal spread of gonorrhea infection in Saskatchewan, Canada.
abstract: Using crude whole-genome assemblies, we analyzed 25 isolates of Neisseria gonorrhoeae by using a high-resolution single nucleotide polymorphism (SNP) approach for nine housekeeping genes, characterizing penA alleles, and antimicrobial susceptibility phenotypes coupled with population structure analysis. Two clonal complexes, characterized by their spatial and geographical persistence, were identified. In addition, the clonal spread of penicillin-resistant/intermediate phenotypes and a novel introduction of the azithromycin resistance phenotype in Saskatchewan, Canada, were ascertained using this method.
authors: Department of Food and Bioproducts Sciences, College of Agriculture, University of Saskatchewan, Saskatoon, Canada.
authors: Sinisa
authors: S
authors: Vidovic
authors: Department of Computer Science, College of Arts and Science, University of Saskatchewan, Saskatoon, Canada.
authors: Carolyn
authors: C
authors: Caron
authors: Vaccine and Infectious Disease Organization, International Vaccine Centre, University of Saskatchewan, Saskatoon, Canada.
authors: Ali
authors: A
authors: Taheri
authors: Department of Microbiology and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
authors: Sidharath D
authors: SD
authors: Thakur
authors: Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
authors: Timothy D
authors: TD
authors: Read
authors: Department of Computer Science, College of Arts and Science, University of Saskatchewan, Saskatoon, Canada.
authors: Anthony
authors: A
authors: Kusalik
authors: Vaccine and Infectious Disease Organization, International Vaccine Centre, University of Saskatchewan, Saskatoon, Canada Department of Microbiology and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, Canada j.dillon@usask.ca.
authors: Jo-Anne R
authors: JA
authors: Dillon
mesh_terms: D006069:Gonorrhea / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission*; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D020641:Polymorphism, Single Nucleotide; D012525:Saskatchewan / Q000453:epidemiology; D017422:Sequence Analysis, DNA / Q000379:methods*