title: Expression and significance of MMP-9 and MDM2 in the oncogenesis of lung cancer in rats.
abstract: OBJECTIVE
  To observe the expression of matrix metalloproteinase-9 (MMP-9) and mouse double minute 2 homolog (MDM2) in the oncogenesis of lung cancer in rats and to explore their clinical value.
  
  
  METHODS
  A total of 140 rats were selected, of which 20 were selected randomly as the control group; and the remaining 120 as the observation group. The observation group was injected with benzopyrene to establish diseases model such as tissue proliferation, abnormal proliferation and lung cancer. Detected the MMP-9 levels of lung tissue by enzyme-linked assay, detected the MDM2 levels of lung tissue by immunochemistry assay.
  
  
  RESULTS
  The MMP-9 and MDM2 expression of the lung cancer group and the abnormal proliferation group were significantly higher than that in the tissue proliferation group and the control group, the difference was significant (P<0.05). And the MDM2 expression of the tissue proliferation group was significantly higher than that in the control group, the difference was significant (P<0.05). There was no significant difference in the MMP-9 expression between the tissue proliferation group and the control group (P>0.05). The MDM2 and MMP-9 expression were increased in turn in the small cell carcinoma, squamous cell carcinoma and adenocarcinoma, the difference was statistically significant (P<0.05). The MMP-9 and MDM2 expressions of stage III and stage IV lung cancer tissue in rats were significant higher than that during stage I and stage II, the difference was significant (P<0.05). There was no significantly different in the MMP-9 and MDM2 expressions between stage III and stage IV(P>0.05), and there is no significant difference of the MMP-9 and MDM2 expressions between stage I and stage II(P>0.05).
  
  
  CONCLUSIONS
  The expression of MMP-9 and MDM2 in lung tissue was associated with lung disease and lung cancer, both of them may be involved in the development and metastasis of lung cancer. Combined detection can be used as therapy and prognostic indicators for lung cancer.
authors: Department of Oncology, Daqing Oilfield General Hospital, Daqing 163000, Heilongjiang, China. Electronic address: Shirley830212@126.com.
authors: De-Hui
authors: DH
authors: Zhang
authors: Department of Oncology, Daqing Oilfield General Hospital, Daqing 163000, Heilongjiang, China.
authors: Liang-Yu
authors: LY
authors: Zhang
authors: Department of Oncology, Daqing Oilfield General Hospital, Daqing 163000, Heilongjiang, China.
authors: Dong-Jie
authors: DJ
authors: Liu
authors: Department of Oncology, Daqing Oilfield General Hospital, Daqing 163000, Heilongjiang, China.
authors: Fang
authors: F
authors: Yang
authors: Department of Oncology, Daqing Oilfield General Hospital, Daqing 163000, Heilongjiang, China.
authors: Jin-Zao
authors: JZ
authors: Zhao
mesh_terms: D000818:Animals; D008175:Lung Neoplasms / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis* / Q000378:metabolism; D009367:Neoplasm Staging; D051736:Proto-Oncogene Proteins c-mdm2 / Q000032:analysis* / Q000378:metabolism; D051381:Rats
chemical_list: C497208:Mdm2 protein, rat; D051736:Proto-Oncogene Proteins c-mdm2; D020780:Matrix Metalloproteinase 9
keywords: Adenocarcinoma; Lung cancer; MDM2; MMP-9