title: Introducing a combinatorial DNA-toolbox platform constituting defined protein-based biohybrid-materials.
abstract: The access to defined protein-based material systems is a major challenge in bionanotechnology and regenerative medicine. Exact control over sequence composition and modification is an important requirement for the intentional design of structure and function. Herein structural- and matrix proteins provide a great potential, but their large repetitive sequences pose a major challenge in their assembly. Here we introduce an integrative "one-vector-toolbox-platform" (OVTP) approach which is fast, efficient and reliable. The OVTP allows for the assembly, multimerization, intentional arrangement and direct translation of defined molecular DNA-tecton libraries, in combination with the selective functionalization of the yielded protein-tecton libraries. The diversity of the generated tectons ranges from elastine-, resilin, silk- to epitope sequence elements. OVTP comprises the expandability of modular biohybrid-materials via the assembly of defined multi-block domain genes and genetically encoded unnatural amino acids (UAA) for site-selective chemical modification. Thus, allowing for the modular combination of the protein-tecton library components and their functional expansion with chemical libraries via UAA functional groups with bioorthogonal reactivity. OVTP enables access to multitudes of defined protein-based biohybrid-materials for self-assembled superstructures such as nanoreactors and nanobiomaterials, e.g. for approaches in biotechnology and individualized regenerative medicine.
authors: Freiburg Institute for Advanced Studies (FRIAS), School of Soft Matter Research, University of Freiburg, Albertstr. 19, 79104, Germany; Institute for Macromolecular Chemistry, Univ. of Freiburg, Stefan-Meier-Str. 31, 79104 Freiburg, Germany; Faculty of Chemistry and Pharmacy, Univ. of Freiburg, Fahnenbergplatz, 79085 Freiburg, Germany. Electronic address: Matthias.Huber@frias.uni-freiburg.de.
authors: Matthias C
authors: MC
authors: Huber
authors: Freiburg Institute for Advanced Studies (FRIAS), School of Soft Matter Research, University of Freiburg, Albertstr. 19, 79104, Germany; Institute for Macromolecular Chemistry, Univ. of Freiburg, Stefan-Meier-Str. 31, 79104 Freiburg, Germany; Faculty of Biology, Univ. of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
authors: Andreas
authors: A
authors: Schreiber
authors: Freiburg Institute for Advanced Studies (FRIAS), School of Soft Matter Research, University of Freiburg, Albertstr. 19, 79104, Germany; Institute for Macromolecular Chemistry, Univ. of Freiburg, Stefan-Meier-Str. 31, 79104 Freiburg, Germany.
authors: Wiltrud
authors: W
authors: Wild
authors: NMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, Baden-Wuerttemberg, Germany.
authors: Karin
authors: K
authors: Benz
authors: Freiburg Institute for Advanced Studies (FRIAS), School of Soft Matter Research, University of Freiburg, Albertstr. 19, 79104, Germany; Institute for Macromolecular Chemistry, Univ. of Freiburg, Stefan-Meier-Str. 31, 79104 Freiburg, Germany; Faculty of Chemistry and Pharmacy, Univ. of Freiburg, Fahnenbergplatz, 79085 Freiburg, Germany; Faculty of Biology, Univ. of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany; Institute for Pharmaceutical Sciences, Univ. of Freiburg, Albertstr. 25, 79104 Freiburg, Germany; BIOSS Centre for Biological Signalling Studies, Univ. of Freiburg, Schänzlestrasse 18, 79104 Freiburg, Germany. Electronic address: Stefan.Schiller@FRIAS.Uni-Freiburg.de.
authors: Stefan M
authors: SM
authors: Schiller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods; D004549:Elastin / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005109:Extracellular Matrix / Q000737:chemistry* / Q000235:genetics; D015723:Gene Library; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods*; D047011:Silk / Q000737:chemistry / Q000235:genetics
chemical_list: D001672:Biocompatible Materials; D019476:Insect Proteins; D047011:Silk; C030951:resilin; D004549:Elastin
keywords: Biohybrid-materials; ECM (extracellular matrix); Elastin-like-protein; Genetic engineering; Molecular tecton-libraries; Self-assembly