title: Effects of substrate to inoculum ratio on the biochemical methane potential of piggery slaughterhouse wastes.
abstract: The aim of this study was to assess the effect of substrate to inoculum ratio (S/I ratio) on the biochemical methane potential (BMP) and anaerobic biodegradability (Ddeg) of different piggery slaughterhouse wastes, such as piggery blood, intestine residue, and digestive tract content. These wastes were sampled from a piggery slaughterhouse located in Kimje, South Korea. Cumulative methane production curves for the wastes were obtained from the anaerobic batch fermentation having different S/I ratios of 0.1, 0.5, 1.0, and 1.5. BMP and anaerobic biodegradabilities (Ddeg) of the wastes were calculated from cumulative methane production data for the tested conditions. At the lowest S/I ration of 0.1, BMPs of piggery blood, intestine residue, and digestive tract content were determined to be 0.799, 0.848, and 1.076 Nm(3) kg(-1)-VSadded, respectively, which were above the theoretical methane potentials of 0.539, 0.644, and 0.517 Nm(3) kg(-1)-VSadded for blood, intestine residue, and digestive tract content, respectively. However, BMPs obtained from the higher S/I ratios of 0.5, 1.0, and 1.5 were within the theoretical range for all three types of waste and were not significantly different for the different S/I ratios tested. Anaerobic biodegradabilities calculated from BMP data showed a similar tendency. These results imply that, for BMP assay in an anaerobic reactor, the S/I ratio of anaerobic reactor should be above 0.1 and the inoculum should be sufficiently stabilized to avoid further degradation during the assay.
authors: Department of Animal Life and Environment Science, Hankyong National University, Anseong, Gyeonggi, 456-749, Korea .
authors: Young-Man
authors: YM
authors: Yoon
authors: Department of Animal Life and Environment Science, Hankyong National University, Anseong, Gyeonggi, 456-749, Korea .
authors: Seung-Hwan
authors: SH
authors: Kim
authors: Department of Animal Life and Environment Science, Hankyong National University, Anseong, Gyeonggi, 456-749, Korea .
authors: Kook-Sik
authors: KS
authors: Shin
authors: Department of Animal Life and Environment Science, Hankyong National University, Anseong, Gyeonggi, 456-749, Korea .
authors: Chang-Hyun
authors: CH
authors: Kim
keywords: Anaerobic Digestion; Biochemical Methane Potential; Piggery Slaughterhouse Waste; S/I Ratio