title: A critical evaluation of inflammatory markers in Huntington's Disease plasma.
abstract: BACKGROUND
  Huntington's Disease (HD) is a hereditary, progressive neurodegenerative disorder characterised by both neurological and systemic symptoms. In HD, immune changes can be observed before the onset of overt clinical features raising the possibility that inflammatory markers in plasma could be used to track disease progression. It has previously been demonstrated that a widespread, progressive innate immune response is detectable in plasma throughout the course of HD.
  
  
  OBJECTIVE
  The aim of the present study was to investigate the potential of several components of inflammation and innate immunity as plasma biomarkers in HD.
  
  
  METHODS
  We utilised antibody-based detection technologies as well as mass spectrometric quantification, multiple reaction monitoring (MRM-MS).
  
  
  RESULTS
  Levels of several markers previously described as altered in HD, such as clusterin, complement component 4, complement component 9 and α-2 macroglobulin did not differ between healthy controls and HD subjects as measured by Luminex, ELISA or MRM-MS. C-reactive protein was decreased in early HD, while the other immune markers tested were unaltered.
  
  
  CONCLUSIONS
  Although only C-reactive protein was found to be reduced in early HD, some of the inflammatory markers measured correlated with clinical measures.
authors: Department of Experimental Medical Science, Brain Disease Biomarker Unit, Wallenberg Neuroscience Center, Lund University, Lund, Sweden.
authors: Edina
authors: E
authors: Silajdžić
authors: Department of Measurement Technology and Industrial Electrical Engineering, Division Nanobiotechnology, Lund University, Lund, Sweden.
authors: Melinda
authors: M
authors: Rezeli
authors: Department of Measurement Technology and Industrial Electrical Engineering, Division Nanobiotechnology, Lund University, Lund, Sweden.
authors: Ákos
authors: Á
authors: Végvári
authors: Department of Neurodegenerative Disease, UCL Institute of Neurology, London, UK.
authors: Nayana
authors: N
authors: Lahiri
authors: Department of Neurodegenerative Disease, UCL Institute of Neurology, London, UK.
authors: Ralph
authors: R
authors: Andre
authors: Department of Experimental Medical Science, Brain Disease Biomarker Unit, Wallenberg Neuroscience Center, Lund University, Lund, Sweden.
authors: Anna
authors: A
authors: Magnusson-Lind
authors: Department of Clinical Neurosciences, UCL Institute of Neurology, London, UK.
authors: Rajasree
authors: R
authors: Nambron
authors: Department of Clinical Neurosciences, UCL Institute of Neurology, London, UK.
authors: Eirini
authors: E
authors: Kalliolia
authors: Department of Measurement Technology and Industrial Electrical Engineering, Division Nanobiotechnology, Lund University, Lund, Sweden.
authors: György
authors: G
authors: Marko-Varga
authors: Department of Clinical Neurosciences, UCL Institute of Neurology, London, UK.
authors: Thomas T
authors: TT
authors: Warner
authors: Department of Measurement Technology and Industrial Electrical Engineering, Division Nanobiotechnology, Lund University, Lund, Sweden.
authors: Thomas
authors: T
authors: Laurell
authors: Department of Neurodegenerative Disease, UCL Institute of Neurology, London, UK.
authors: Sarah J
authors: SJ
authors: Tabrizi
authors: Department of Experimental Medical Science, Brain Disease Biomarker Unit, Wallenberg Neuroscience Center, Lund University, Lund, Sweden.
authors: Maria
authors: M
authors: Björkqvist
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D002097:C-Reactive Protein / Q000032:analysis / Q000276:immunology; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D006816:Huntington Disease / Q000097:blood* / Q000276:immunology*; D007249:Inflammation / Q000097:blood* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein
keywords: C-reactive protein; Huntington's disease; biomarker; complement; inflammation; innate immunity