title: Molecular cloning, relative expression, and structural analysis of pattern recognition molecule β-glucan binding protein from mangrove crab Episesarma tetragonum.
abstract: A full-length cDNA of a β-glucan binding protein (β-GBP) gene was identified from the mangrove crab Episesarma tetragonum. The open reading frame of the E. tetragonum β-GBP (Epte β-GBP) is 1,167 bp long, encoding a polypeptide of 389 amino acids. The deduced amino acid sequence of Epte β-GBP gene has conserved a potential recognition motif for β-1,3 linkages of polysaccharides and putative RGD (Arg-Gly-Asp) cell adhesion sites. Phylogenetic analysis of the Epte β-GBP gene showed the similarity with β-GBPs of other crustaceans and arthropods. Quantitative RT-PCR results showed the upregulation of Epte β-GBP gene expression in E. tetragonum hemocytes following a 12-H challenge in response to β-glucan (β-G). Epte β-GBP was involved in the regulation and activation of the prophenoloxidase cascade. A three-dimensional structure of active Epte β-GBP was modeled by homology modeling and refined with molecular dynamics simulations. A structural aspect of the protein is discussed based on experimental and theoretical results obtained.
authors: Department of Animal Health and Management, Crustacean Molecular Biology and Genomics Lab, Alagappa University, Karaikudi, Tamil Nadu, India.
authors: Jeyachandran
authors: J
authors: Sivakamavalli
authors: Department of Bioinformatics, Computer Aided Drug Design and Molecular Modeling Lab, Alagappa University, Karaikudi, Tamil Nadu, India.
authors: Chandrabose
authors: C
authors: Selvaraj
authors: Department of Bioinformatics, Computer Aided Drug Design and Molecular Modeling Lab, Alagappa University, Karaikudi, Tamil Nadu, India.
authors: Sanjeev Kumar
authors: SK
authors: Singh
authors: Department of Animal Health and Management, Crustacean Molecular Biology and Genomics Lab, Alagappa University, Karaikudi, Tamil Nadu, India.
authors: Baskaralingam
authors: B
authors: Vaseeharan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics*; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods*; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data
chemical_list: D002352:Carrier Proteins; D037102:Lectins; C037937:glucan-binding proteins
keywords: RMSD; cloning; docking; molecular simulation; qRT-PCR; β-GBP