title: Proteomic analisys of protein extraction during hemofiltration with on-line endogenous reinfusion (HFR) using different polysulphone membranes.
abstract: In end-stage renal disease patients, extracorporeal dialytic therapy is not able to prevent the accumulation of toxins related to the uremic syndrome, a severe complication that increases morbidity and mortality rate. In this paper, hemoFiltration with on-line Reinfusion (HFR) architecture is used to evaluate the effect of a more permeable membrane on the extraction of medium-high molecular weight molecules. The aim of this study was to compare two polysulphone membranes for convective chamber: polyphenylene High Flux (pHF) and polyphenylene Super High-Flux (pSHF). Fourteen patients were subjected to HFR with pHF and pSHF membranes and ultra filtrate (UF) samples were collected to evaluate molecular weight cut-off (MWCO) and to identify extracted proteins. Furthermore, image analysis software was used in order to evaluate change in protein extraction during the dialysis. The quantification of four proteins by immunoassay demonstrates a higher permeability of pSHF membrane. Two-dimensional electrophoresis (2-DE) gels showed, for both membranes, the greater number of protein spots at 235 min. Some of the identified proteins, involved in nephropathic disease complications, were compared to assess differences in extraction during dialytic treatment by PDQuest analysis. UF proteomic analysis demonstrated a different behavior for the two membranes; pHF membrane was more permeable at the beginning of HFR treatment (15 min), while pSHF membrane at the end of treatment (235 min). Proteomic analysis is a suitable approach to investigate the behavior of different membranes during dialysis. Results indicated that pSHF membrane offers the higher permeability, and showed higher efficiency in removal of middle molecules related to uremic syndrome.
authors: Emanuela
authors: E
authors: Monari
authors: Department of Diagnostic, Clinical and Public Health Medicine, University of Modena and Reggio Emilia, Via del Pozzo, 71-41124, Modena, Italy, emanuela.monari@unimore.it.
authors: Monari
authors: M
authors: Emanuela
authors: Aurora
authors: A
authors: Cuoghi
authors: Cuoghi
authors: C
authors: Aurora
authors: Elisa
authors: E
authors: Bellei
authors: Bellei
authors: B
authors: Elisa
authors: Stefania
authors: S
authors: Bergamini
authors: Bergamini
authors: B
authors: Stefania
authors: Marialuisa
authors: M
authors: Caiazzo
authors: Caiazzo
authors: C
authors: Marialuisa
authors: Filippo
authors: F
authors: Aucella
authors: Aucella
authors: A
authors: Filippo
authors: Carmelo
authors: C
authors: Loschiavo
authors: Loschiavo
authors: L
authors: Carmelo
authors: Luca
authors: L
authors: Corazza
authors: Corazza
authors: C
authors: Luca
authors: Giuseppe
authors: G
authors: Palladino
authors: Palladino
authors: P
authors: Giuseppe
authors: Luisa
authors: L
authors: Sereni
authors: Sereni
authors: S
authors: Luisa
authors: Mauro
authors: M
authors: Atti
authors: Atti
authors: A
authors: Mauro
authors: Aldo
authors: A
authors: Tomasi
authors: Tomasi
authors: T
authors: Aldo
mesh_terms: D000368:Aged; D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D006440:Hemofiltration / Q000295:instrumentation*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000534:rehabilitation; D008297:Male; D008422:Materials Testing; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D020543:Proteome / Q000737:chemistry* / Q000302:isolation & purification*; D013450:Sulfones / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D008567:Membranes, Artificial; D011108:Polymers; D020543:Proteome; D013450:Sulfones; C017662:polysulfone P 1700