title: Improvement of production of lipopeptide antibiotic iturin A using fish protein.
abstract: To enhance the production of lipopeptide antibiotic iturin A, nutrient contents of the culture mediums were investigated in both submerged and biofilm fermentations. As a carbon source maltose and as nitrogen source, fish protein was used. In submerged fermentation maltose uptake was found lower (12%) compared to biofilm fermentation (15%) that was associated with higher cellular growth in biofilm. However, requirement of nitrogen (fish protein) concentration was found similar in both submerged and biofilm fermentations. Production of iturin A in submerged fermentation with 12% maltose and 5% fish protein was 4450 mg/L, and in biofilm fermentation it was 5050 mg/L when 15% maltose and 5% fish protein was used.
authors: Proximo Biotech, 1-50 Newtown, Dinajpur 5200, Bangladesh.  zohoraus@gmail.com
authors: Umme Salma
authors: US
authors: Zohora
authors: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Savar 1342, Dhaka, Bangladesh.
authors: Mohammad Shahedur
authors: MS
authors: Rahman
authors: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; Agriculture Group, Alps Business Creation Co., Ltd., Kawasaki 210-0855, Japan.
authors: Abdul Wahab
authors: AW
authors: Khan
authors: Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa-city, Wakayama, 649-6493, Japan.
authors: Masahiro
authors: M
authors: Okanami
authors: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa-city, Wakayama, 649-6493, Japan. Electronic address: tano@waka.kindai.ac.jp.
authors: Takashi
authors: T
authors: Ano
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D029941:Fish Proteins / Q000378:metabolism*; D055666:Lipopeptides / Q000096:biosynthesis*; D009584:Nitrogen / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000096:biosynthesis*
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D029941:Fish Proteins; D055666:Lipopeptides; D010456:Peptides, Cyclic; C013579:iturin A; D002244:Carbon; D009584:Nitrogen
keywords: Bacillus subtilis; biofilm fermentation; iturin A; lipopeptide antibiotic