title: Human Papillomavirus Deoxyribonucleic Acid may not be Detected in Non-genital Benign Papillomatous Skin Lesions by Polymerase Chain Reaction.
abstract: BACKGROUND
  Papillomatosis is a known histopathologic pattern usually seen in human papillomavirus (HPV) infection and verruca vulgaris is the typical example. This pattern is also detected in some other benign cutaneous lesions such as nevus sebaceous (NS), seborrheic keratosis (SK), trichilemmoma (TL) and inverted follicular keratosis (IFK). The association between papillomatous lesions and HPV infection is questionable.
  
  
  OBJECTIVE
  The objective of this study was to investigate the presence of HPV deoxyribonucleic acid (DNA) in non-genital benign papillomatous skin lesions (NS, SK, TL and IFK) by polymerase chain reaction (PCR).
  
  
  METHODS
  A total of 100 specimens of non-genital NS, SK, TL and IFK were retrieved from archives of Dermatopathology Department of Razi Hospital, between 2003 and 2010. The conventional PCR using consensus GP5+/GP6+ primer and hydroxymethylbilane synthase gene as inner control was performed.
  
  
  RESULTS
  PCR for HPV DNA revealed no positive results in any of 28 seborrheic keratosis (SK), 28 nevus sebaceous (NS), 28 inverted follicular keratosis (IFK) and 13 trichilemmoma (TL) studied specimens.
  
  
  CONCLUSIONS
  Papillomatosis is usually a characteristic pattern of HPV infection. However, we found no association between HPV infection and non-genital benign papillomatous lesions.
authors: Department of Dermatopathology, Tehran University of Medical Sciences, Tehran, Iran.
authors: Kamyab Hesari
authors: KH
authors: Kambiz
authors: Department of Dermatopathology, Tehran University of Medical Sciences, Tehran, Iran.
authors: Davoodi
authors: D
authors: Kaveh
authors: Department of Dermatopathology, Tehran University of Medical Sciences, Tehran, Iran.
authors: Damavandi
authors: D
authors: Maede
authors: Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Ayatollahi
authors: A
authors: Hossein
authors: Department of Dermatopathology, Tehran University of Medical Sciences, Tehran, Iran.
authors: Aghazadeh
authors: A
authors: Nessa
authors: Department of Dermatopathology, Tehran University of Medical Sciences, Tehran, Iran.
authors: Rahbar
authors: R
authors: Ziba
authors: Department of Dermatopathology, Tehran University of Medical Sciences, Tehran, Iran.
authors: Ghanadan
authors: G
authors: Alireza
keywords: Human papillomavirus; inverted follicular keratosis; nevus sebaceous; papillomatosis; polymerase chain reaction; seborrheic keratosis; trichilemmoma