title: Early induction of ADAMTS 1, -4, -5 and -9 in IL-stimulated mouse astrocytes.
abstract: OBJECTIVE
  Astrocytes and extracellular matrix molecules have important roles in regulating synaptic functions between neurons in the central nervous system. However, under pathological conditions, these constituents are activated to form glial scar that is thought to be harmful for neuronal regeneration. The aim of this study was to evaluate the expression pattern of ADAMTS1, -4, -5 and -9 in IL-1 stimulated astrocyte cultures obtained from postnatal day zero mouse brains.
  
  
  METHODS
  Real time PCR analyses were performed.
  
  
  RESULTS
  An overexpression of ADAMTS1, -4, -5 and -9 at the 3-h time point after IL-1 stimulation was found. IL-1 stimulation induced aggrecaneses and this effect was time dependent. Maximum increase was detected at 3-h (six fold increase). Interestingly the expression of ADAMTS1 and -4 appeared to be at the highest expression level but the ADAMTS5 and ADAMTS9 expression level was much weaker (three times and two times respectively).
  
  
  CONCLUSIONS
  To the best of our knowledge, this is the first report demonstrating induction of ADAMTS in IL-1 induced astrocytes. Aggrecanases may play a role in tissue destruction in the progression of central nervous system (CNS) injury and they are differentially expressed in mouse CNS, suggesting a critical role in the pathogenesis of CNS injury. This can be a very crucial aetiologic factor for some neuropsychiatric disorders.
authors: Istanbul Univetsity, School of Medicine, Department of Child and Adolescent Psychiatry, Istanbul, Turkey.
authors: Osman
authors: O
authors: Abali
authors: Emre Cemal
authors: EC
authors: Gokce
authors: Berker
authors: B
authors: Cemil
authors: Bulent
authors: B
authors: Erdogan
authors: Tomoko
authors: T
authors: Yonezawa
authors: Kadir
authors: K
authors: Demircan
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis*; D000071097:ADAMTS1 Protein; D000071121:ADAMTS4 Protein; D000071116:ADAMTS5 Protein; D000071119:ADAMTS9 Protein; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology*; D051379:Mice; D016133:Polymerase Chain Reaction; D061251:Primary Cell Culture; D011348:Procollagen N-Endopeptidase / Q000096:biosynthesis*
chemical_list: D007375:Interleukin-1; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; D000071116:ADAMTS5 Protein; D000071119:ADAMTS9 Protein; C486323:Adamts1 protein, mouse; C498489:Adamts5 protein, mouse; C545434:Adamts9 protein, mouse; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein