title: Detection of metronidazole and ronidazole from environmental samples by surface enhanced Raman spectroscopy.
abstract: In this study, the surface enhanced Raman spectra (SERS) of two prohibited veterinary drugs, metronidazole (MNZ) and ronidazole (RNZ), have been acquired, and compared to the theoretically calculated spectra using density function theory (DFT). The experimental Raman and SERS spectra of MNZ and RNZ exhibit high resemblance with the DFT calculations. SERS detection of MNZ and RNZ from standard solutions as well as real environmental samples (tap, lake, swamp waters and soil) was performed on highly sensitive and reproducible silver nanorod array substrates. The limits of detection for MNZ and RNZ are 10 and 1 µg/mL in methanol and ultra-pure water, respectively, and 10-50 µg/mL in the environmental samples. The SERS-based method demonstrates its potential as a rapid, simple, and inexpensive means for the onsite screening of banned antibiotics from the aquatic and sediment environments, with minimal requirement for sample pretreatment.
authors: Department of Physics and Astronomy, University of Georgia, USA; Nanoscale Science and Engineering Center, University of Georgia, USA; School of Physics and Electronic Engineering, Jiangsu Normal University, China; Jiangsu Province Key Laboratory of Advanced Laser Materials and Devices, China. Electronic address: hancq@jsnu.edu.cn.
authors: Caiqin
authors: C
authors: Han
authors: Nanoscale Science and Engineering Center, University of Georgia, USA; Department of Food Science and Technology, University of Georgia, USA. Electronic address: jingchen@uga.edu.
authors: Jing
authors: J
authors: Chen
authors: Nanoscale Science and Engineering Center, University of Georgia, USA; Department of Food Science and Technology, University of Georgia, USA.
authors: Xiaomeng
authors: X
authors: Wu
authors: Nanoscale Science and Engineering Center, University of Georgia, USA; Department of Food Science and Technology, University of Georgia, USA.
authors: Yao-Wen
authors: YW
authors: Huang
authors: Department of Physics and Astronomy, University of Georgia, USA; Nanoscale Science and Engineering Center, University of Georgia, USA.
authors: Yiping
authors: Y
authors: Zhao
mesh_terms: D016002:Discriminant Analysis; D060766:Drinking Water / Q000032:analysis / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods; D005618:Fresh Water / Q000032:analysis / Q000737:chemistry; D060106:Lakes / Q000737:chemistry; D016018:Least-Squares Analysis; D008795:Metronidazole / Q000032:analysis*; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D012385:Ronidazole / Q000032:analysis*; D012987:Soil / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis; D062066:Water Resources / Q000032:analysis*; D014881:Water Supply / Q000032:analysis
chemical_list: D060766:Drinking Water; D012987:Soil; D014874:Water Pollutants, Chemical; D008795:Metronidazole; D012385:Ronidazole
keywords: Antibiotics; Nitroimidazoles; Silver nanorods; Surface enhanced Raman spectroscopy