title: 5,10,15,20-tetrakis(4-carboxyl phenyl)porphyrin-CdS nanocomposites with intrinsic peroxidase-like activity for glucose colorimetric detection.
abstract: Here, we describe the design of a novel mimic peroxidase, nanocomposites composed by 5,10,15,20-tetrakis(4-carboxyl phenyl)-porphyrin (H2TCPP) and cadmium sulfide (CdS). The H2TCPP-CdS nanocomposites can catalyze oxidation of substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2 and form a blue product which can be seen by the naked eye in 5 min. The mechanism of the catalytic reaction originated from the generation of hydroxyl radical (·OH), which is a powerful oxidizing agent to oxidize TMB to produce a blue product. Then, we developed a colorimetric method that is highly sensitive and selective to detect glucose, combined with glucose oxidase (GOx). The proposed method allowed the detection of H2O2 concentration in the range of 4×10(-6)-1.4×10(-5)M and glucose in the range of 1.875×10(-5)-1×10(-4)M with detectable H2O2 concentration as low as 4.6×10(-7)M and glucose as low as 7.02×10(-6)M, respectively. The results provided the theoretical basis of practical application in glucose detecting and peroxidase mimetic enzymes.
authors: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China. Electronic address: qyliu@sdust.edu.cn.
authors: Qingyun
authors: Q
authors: Liu
authors: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors: Qingyan
authors: Q
authors: Jia
authors: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors: Renren
authors: R
authors: Zhu
authors: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors: Qian
authors: Q
authors: Shao
authors: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors: Dongmei
authors: D
authors: Wang
authors: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors: Peng
authors: P
authors: Cui
authors: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: jchge2010@mail.ipc.ac.cn.
authors: Jiechao
authors: J
authors: Ge
mesh_terms: D001560:Benzidines / Q000378:metabolism; D019187:Cadmium Compounds / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D057230:Limit of Detection; D008665:Metalloporphyrins / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism*; D013440:Sulfides / Q000737:chemistry*
chemical_list: D001560:Benzidines; D019187:Cadmium Compounds; D008665:Metalloporphyrins; D013440:Sulfides; C034939:cadmium sulfide; C021758:3,3',5,5'-tetramethylbenzidine; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D009195:Peroxidase; D005947:Glucose
keywords: CdS; Colorimetric; Glucose; Peroxidase; Porphyrin