title: Case Report: Detection and quantification of tumor cells in peripheral blood and ascitic fluid from a metastatic esophageal cancer patient using the CellSearch (®) technology.
abstract: Analysis of ascitic fluid should help to identify and characterize malignant cells in gastrointestinal cancer. However, despite a high specificity, the sensitivity of traditional ascitic fluid cytology remains insufficient, at around 60%. Since 2004 the CellSearch (®) technology has shown its advantages in the detection of circulating tumor cells (CTCs) in peripheral blood, which can perform an accurate diagnosis and molecular analysis at the same time. To our knowledge, no previous study has explored the potential utility of this technology for the detection and quantification of tumor cells in ascitic fluid samples. Herein we report a case of metastatic esophageal adenocarcinoma in a 70-year-old man presenting with dysphagia and a large amount of fluid in the peritoneal cavity. Analysis of a peripheral blood sample and ascites sample with the CellSearch (®) technology both revealed the presence of putative tumor cells that were positive for epithelial cell adhesion molecule (EpCAM) and cytokeratin (CK) expression. This study confirmed the hematogenous dissemination of esophageal cancer by the detection of circulating tumor cells in the peripheral blood, and is the first to demonstrate that tumor cells can be identified in ascitic fluid by using CellSearch (®) technology.
authors: CHU Nancy, Nancytomique, Laboratoire d'Immunologie, Pôle Laboratoires, Vandoeuvre lès Nancy, France and Université Henri Poincaré, Faculté de Médecine, EA4369 RHEM and UMR7039 CRAN-CNRS, Vandœuvre-lès-Nancy, France.
authors: Qian
authors: Q
authors: Tu
authors: CHU Nancy, Nancytomique, Laboratoire d'Immunologie, Pôle Laboratoires, Vandoeuvre lès Nancy, France and Université Henri Poincaré, Faculté de Médecine, EA4369 RHEM and UMR7039 CRAN-CNRS, Vandœuvre-lès-Nancy, France.
authors: Marcelo De Carvalho
authors: Mde C
authors: Bittencourt
authors: CHU Nancy, Nancytomique, Laboratoire d'Immunologie, Pôle Laboratoires, Vandoeuvre lès Nancy, France and Université Henri Poincaré, Faculté de Médecine, EA4369 RHEM and UMR7039 CRAN-CNRS, Vandœuvre-lès-Nancy, France.
authors: Huili
authors: H
authors: Cai
authors: Service D'Anatomie et Cytologie Pathologiques, CHU Nancy-Brabois, Vandœuvre-lès-Nancy, France.
authors: Claire
authors: C
authors: Bastien
authors: Service D'Anatomie et Cytologie Pathologiques, CHU Nancy-Brabois, Vandœuvre-lès-Nancy, France.
authors: Camille
authors: C
authors: Lemarie-Delaunay
authors: CHU Nancy, Nancytomique, Laboratoire d'Immunologie, Pôle Laboratoires, Vandoeuvre lès Nancy, France and Université Henri Poincaré, Faculté de Médecine, EA4369 RHEM and UMR7039 CRAN-CNRS, Vandœuvre-lès-Nancy, France.
authors: Marie C
authors: MC
authors: Bene
authors: CHU Nancy, Nancytomique, Laboratoire d'Immunologie, Pôle Laboratoires, Vandoeuvre lès Nancy, France and Université Henri Poincaré, Faculté de Médecine, EA4369 RHEM and UMR7039 CRAN-CNRS, Vandœuvre-lès-Nancy, France.
authors: Gilbert C
authors: GC
authors: Faure