title: Immunohistochemical tools and techniques to visualize Notch in Drosophila melanogaster.
abstract: The ability to accurately visualize proteins in Drosophila tissues is critical for studying their abundance and localization relative to the morphology of cells during tissue development and homeostasis. Here we describe the procedure to visualize Notch localization in whole-mount preparations of several Drosophila organs using confocal microscopy. The use of monoclonal antibodies directed to distinct portions of Notch allows one to follow the fate of the receptor during constitutive and inductive processes. The protocol described here can be used to co-label with antibodies recognizing markers of subcellular compartments in wild-type as well as mutant tissues.
authors: Istituto FIRC di Oncologia Molecolare (IFOM), IFOM-IEO Campus, via Adamello 16, 20139, Milano, Italy.
authors: Emiliana
authors: E
authors: Tognon
authors: Thomas
authors: T
authors: Vaccari
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000032:analysis*; D004331:Drosophila melanogaster / Q000737:chemistry / Q000648:ultrastructure*; D007150:Immunohistochemistry / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D051880:Receptors, Notch / Q000032:analysis*
chemical_list: D029721:Drosophila Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch