title: Directly utilizing an endogenous gene to dissect regulatory elements in the biosynthetic gene cluster of nosiheptide.
abstract: The regulatory elements for nosiheptide biosynthesis were identified by a novel host-vector system with an endogenous gene within the biosynthetic gene cluster as a reporter gene. The present study offers a rapid and reliable method for the identification of regulatory elements in the biosynthesis of various bioactive natural products.
authors: State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology, China Pharmaceutical University, 24 Tongjia Street, Nanjing, Jiangsu Province 210009, China. yjchen@cpu.edu.cn shuzhenwang@cpu.edu.cn.
authors: Min
authors: M
authors: Ma
authors: Yanjiu
authors: Y
authors: Xue
authors: Weiying
authors: W
authors: Liu
authors: Hong
authors: H
authors: Zhang
authors: Lingyi
authors: L
authors: Kong
authors: Shuzhen
authors: S
authors: Wang
authors: Yijun
authors: Y
authors: Chen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D055162:Biocatalysis; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D013844:Thiazoles; C014134:nosiheptide