title: Plant-derived antimicrobials reduce E. coli O157:H7 virulence factors critical for colonization in cattle gastrointestinal tract in vitro.
abstract: This study investigated the effect of subinhibitory concentrations (SIC) of five plant-derived antimicrobials (PDAs), namely, trans cinnamaldehyde, eugenol, carvacrol, thymol, and β-resorcylic acid, on E. coli O157:H7 (EHEC) attachment and invasion of cultured bovine colonic (CO) and rectoanal junction (RAJ) epithelial cells. In addition, PDAs' effect on EHEC genes critical for colonization of cattle gastrointestinal tract (CGIT) was determined in bovine rumen fluid (RF) and intestinal contents (BICs). Primary bovine CO and RAJ epithelial cells were established and were separately inoculated with three EHEC strains with or without (control) SIC of each PDA. Following incubation, EHEC that attached and invaded the cells were determined. Furthermore, the expression of EHEC genes critical for colonization in cattle was investigated using real-time, quantitative polymerase chain reaction in RF and BICs. All the PDAs decreased EHEC invasion of CO and RAJ epithelial cells (P < 0.05). The PDAs also downregulated (P < 0.05) the expression of EHEC genes critical for colonization in CGIT. Results suggest that the PDAs could potentially be used to control EHEC colonization in cattle; however follow-up in vivo studies in cattle are warranted.
authors: Department of Poultry Science, Texas A&M University, College Station, TX 77843, USA.
authors: Sangeetha
authors: S
authors: Ananda Baskaran
authors: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors: Kumar
authors: 0000-0001-5966-7852
authors: K
authors: Venkitanarayanan
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D002417:Cattle; D015169:Colony Count, Microbial; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity*; D019856:Ethanolamine / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000382:microbiology* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D009093:Mucus / Q000378:metabolism; D010944:Plants / Q000737:chemistry*; D012417:Rumen / Q000382:microbiology; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D037521:Virulence Factors; D019856:Ethanolamine