title: Higher number of pentosidine cross-links induced by ribose does not alter tissue stiffness of cancellous bone.
abstract: The role of mature collagen cross-links, pentosidine (Pen) cross-links in particular, in the micromechanical properties of cancellous bone is unknown. The aim of this study was to examine nonenzymatic glycation effects on tissue stiffness of demineralized and non-demineralized cancellous bone. A total of 60 bone samples were derived from mandibular condyles of six pigs, and assigned to either control or experimental groups. Experimental handling included incubation in phosphate buffered saline alone or with 0.2M ribose at 37°C for 15 days and, in some of the samples, subsequent complete demineralization of the sample surface using 8% EDTA. Before and after experimental handling, bone microarchitecture and tissue mineral density were examined by means of microcomputed tomography. After experimental handling, the collagen content and the number of Pen, hydroxylysylpyridinoline (HP), and lysylpyridinoline (LP) cross-links were estimated using HPLC, and tissue stiffness was assessed by means of nanoindentation. Ribose treatment caused an up to 300-fold increase in the number of Pen cross-links compared to nonribose-incubated controls, but did not affect the number of HP and LP cross-links. This increase in the number of Pen cross-links had no influence on tissue stiffness of both demineralized and nondemineralized bone samples. These findings suggest that Pen cross-links do not play a significant role in bone tissue stiffness.
authors: Dept. of Orthodontics, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands; Dept. of Oral Cell Biology and Functional Anatomy, MOVE Research Institute, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands. Electronic address: n.willems@acta.nl.
authors: Nop M B K
authors: NM
authors: Willems
authors: Dept. of Oral Cell Biology and Functional Anatomy, MOVE Research Institute, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands.
authors: Geerling E J
authors: GE
authors: Langenbach
authors: Dept. of Metabolic Health Research, TNO, P.O. Box 2215, 2301 CE Leiden, The Netherlands.
authors: Reinout
authors: R
authors: Stoop
authors: Dept. of Mechanical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
authors: Jaap M J
authors: JM
authors: den Toonder
authors: Dept. of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
authors: Lars
authors: L
authors: Mulder
authors: Dept. of Orthodontics, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands.
authors: Andrej
authors: A
authors: Zentner
authors: Dept. of Oral Cell Biology and Functional Anatomy, MOVE Research Institute, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands.
authors: Vincent
authors: V
authors: Everts
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000737:chemistry; D001696:Biomechanical Phenomena; D003094:Collagen / Q000737:chemistry; D005260:Female; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry; D008335:Mandibular Condyle / Q000737:chemistry* / Q000502:physiology*; D049329:Nanostructures / Q000737:chemistry; D018583:Pliability / Q000502:physiology; D012266:Ribose / Q000737:chemistry*; D013552:Swine
chemical_list: D012266:Ribose; D003094:Collagen; D001120:Arginine; C062187:pentosidine; D008239:Lysine
keywords: Biomechanics; Cancellous bone; Collagen cross-links; Glycation; Nanoindentation