title: Differential targeting of feline photoreceptors by recombinant adeno-associated viral vectors: implications for preclinical gene therapy trials.
abstract: The cat is emerging as a promising large animal model for preclinical testing of retinal dystrophy therapies, for example, by gene therapy. However, there is a paucity of studies investigating viral vector gene transfer to the feline retina. We therefore sought to study the tropism of recombinant adeno-associated viral (rAAV) vectors for the feline outer retina. We delivered four rAAV serotypes: rAAV2/2, rAAV2/5, rAAV2/8 and rAAV2/9, each expressing green fluorescent protein (GFP) under the control of a cytomegalovirus promoter, to the subretinal space in cats and, for comparison, mice. Cats were monitored for gene expression by in vivo imaging and cellular tropism was determined using immunohistochemistry. In cats, rAAV2/2, rAAV2/8 and rAAV2/9 vectors induced faster and stronger GFP expression than rAAV2/5 and all vectors transduced the retinal pigment epithelium (RPE) and photoreceptors. Unlike in mice, cone photoreceptors in the cat retina were more efficiently transduced than rod photoreceptors. In mice, rAAV2/2 only transduced the RPE whereas the other vectors also transduced rods and cones. These results highlight species differences in cellular tropism of rAAV vectors in the outer retina. We conclude that rAAV serotypes are suitable for use for retinal gene therapy in feline models, particularly when cone photoreceptors are the target cell.
authors: Small Animal Clinical Sciences, Michigan State University, East Lansing, MI, USA.
authors: A L
authors: AL
authors: Minella
authors: Small Animal Clinical Sciences, Michigan State University, East Lansing, MI, USA.
authors: F M
authors: FM
authors: Mowat
authors: F M Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA, USA.
authors: K L
authors: KL
authors: Willett
authors: Diagnostic Center for Population and Animal Health, Michigan State University, Lansing, MI, USA.
authors: D
authors: D
authors: Sledge
authors: Small Animal Clinical Sciences, Michigan State University, East Lansing, MI, USA.
authors: J T
authors: JT
authors: Bartoe
authors: F M Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA, USA.
authors: J
authors: J
authors: Bennett
authors: Small Animal Clinical Sciences, Michigan State University, East Lansing, MI, USA.
authors: S M
authors: SM
authors: Petersen-Jones
mesh_terms: D000818:Animals; D002415:Cats; D000229:Dependovirus / Q000235:genetics / Q000502:physiology*; D005260:Female; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D056965:Injections, Intraocular; D008297:Male; D051379:Mice; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism* / Q000821:virology; D017948:Retinal Rod Photoreceptor Cells / Q000378:metabolism* / Q000821:virology; D014161:Transduction, Genetic; D056189:Viral Tropism
chemical_list: D049452:Green Fluorescent Proteins