title: Exploring the interaction of a micelle entrapped biologically important proton transfer probe with the model transport protein bovine serum albumin.
abstract: This article describes the interaction of a micelle entrapped pharmaceutically important isoindole fused imidazole derivative, namely, 1-(2-hydroxy-5-methyl-phenyl)-3,5-dioxo-1H-imidazo-[3,4-b] isoindole (ADII), with the model transport protein bovine serum albumin (BSA). Different spectroscopic techniques such as steady state absorption, emission, circular dichroism, dynamic light scattering, etc., have been employed to explore preferential interaction of this drug template with micelles and protein BSA. Binding of ADII with BSA is found to be enormously modified when it is released from the micellar environment. The binding constant of the ADII-BSA complex is reduced when the probe is released from anionic SDS micelle, whereas the binding is observed to be strengthened in cationic CTAB micellar medium due to the formation of a 1:2 complex (ADII-BSA). Time-resolved studies also support our steady state findings that the released drug from the micellar environment is found to be strongly bound with the protein BSA. Circular dichroism (CD) and dynamic light scattering (DLS) study reveals that the secondary structure of BSA gets some stabilization in SDS medium after binding of drug template to protein. The probable binding location of the probe within the protein cavity (hydrophilic subdomain IA) has been explored from an AutoDock-based blind docking simulation study.
authors: Department of Chemistry, University of Calcutta , 92 A. P. C. Road, Kolkata, 700009, India.
authors: Debarati
authors: D
authors: Ray
authors: Ashis
authors: A
authors: Kundu
authors: Animesh
authors: A
authors: Pramanik
authors: Nikhil
authors: N
authors: Guchhait
mesh_terms: D000818:Animals; D002021:Buffers; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles / Q000737:chemistry*; D007769:Lactams / Q000737:chemistry; D008823:Micelles; D062105:Molecular Docking Simulation; D017830:Octoxynol / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D011522:Protons; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: C000605264:1-(2-hydroxy-5-methylphenyl)-3,5-dioxo-1H-imidazo(3,4-b)isoindole; D002021:Buffers; D002593:Cetrimonium Compounds; D007093:Imidazoles; D007211:Indoles; D054833:Isoindoles; D007769:Lactams; D008823:Micelles; D011522:Protons; D013501:Surface-Active Agents; D014867:Water; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol; D000077286:Cetrimonium