title: Mapping of protein binding RNA elements.
abstract: The RNA electrophoretic mobility shift assay is a very versatile method to study a broad spectrum of RNA-protein interactions. The technique is based on the separation of protein RNA mixtures through a native acrylamide gel. Compared to unbound RNA, RNA-protein complexes migrate slower through the gel resulting in a mass shift. The RNA EMSA can be used to identify unknown RNA-protein complexes, to map the RNA binding site of single proteins or determine the specificity of RNA-protein complexes using specific antibodies resulting in retarded migration through the gel.
authors: Molecular and Translational Cardiology, Medical School Hannover, Carl-Neuberg-Straße 1, 30625, Hannover, Germany, Reboll.Marc@mh-hannover.de.
authors: Marc R
authors: MR
authors: Reboll
mesh_terms: D002874:Chromosome Mapping; D024202:Electrophoretic Mobility Shift Assay; D011485:Protein Binding; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: D016601:RNA-Binding Proteins; D012313:RNA