title: Combined pretreatment using alkaline hydrothermal and ball milling to enhance enzymatic hydrolysis of oil palm mesocarp fiber.
abstract: Hydrothermal pretreatment of oil palm mesocarp fiber was conducted in tube reactor at treatment severity ranges of log Ro = 3.66-4.83 and partial removal of hemicellulose with migration of lignin was obtained. Concerning maximal recovery of glucose and xylose, 1.5% NaOH was impregnated in the system and subsequent ball milling treatment was employed to improve the conversion yield. The effects of combined hydrothermal and ball milling pretreatments were evaluated by chemical composition changes by using FT-IR, WAXD and morphological alterations by SEM. The successful of pretreatments were assessed by the degree of enzymatic digestibility of treated samples. The highest xylose and glucose yields obtained were 63.2% and 97.3% respectively at cellulase loadings of 10 FPU/g-substrate which is the highest conversion from OPMF ever reported.
authors: Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan; Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: rafein-zakaria@aist.go.jp.
authors: Mohd Rafein
authors: MR
authors: Zakaria
authors: Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.
authors: Satoshi
authors: S
authors: Hirata
authors: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors: Mohd Ali
authors: MA
authors: Hassan
mesh_terms: D028023:Arecaceae / Q000737:chemistry* / Q000187:drug effects; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D003460:Crystallization; D005638:Fruit / Q000737:chemistry* / Q000187:drug effects; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D010316:Particle Size; D012972:Sodium Hydroxide / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000494:pharmacology*; D014994:Xylose / Q000032:analysis; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D014867:Water; D012972:Sodium Hydroxide; D014994:Xylose; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose
keywords: Alkaline hydrothermal; Ball mill; Glucose; Oil palm mesocarp fiber; Xylose