title: Analysis of ENPP2 in the Uterine Endometrium of Pigs Carrying Somatic Cell Nuclear Transfer Cloned Embryos.
abstract: Somatic cell nuclear transfer (SCNT) is a useful tool for animal cloning, but the efficiency of producing viable offspring by SCNT is very low. To improve this efficiency in the production of cloned pigs, it is critical to understand the interactions between uterine function and cloned embryos during implantation. Lysophosphatidic acid (LPA) is a lipid mediator that plays an important role in the establishment of pregnancy in pigs; however, LPA production in the uterine endometrium of pigs carrying SCNT-cloned conceptuses has not been determined. Therefore, we investigated expression of ENPP2, an LPA-generating enzyme, in the uterine endometrium of gilts with conceptuses derived from SCNT during the implantation period. Uterine endometrial tissue and uterine flushing were obtained from gilts carrying SCNT-derived conceptuses and from gilts carrying conceptuses resulting from natural mating on d 12 of pregnancy. Our results demonstrated no difference in the level of ENPP2 mRNA expression in the uterine endometrium between gilts carrying SCNT-derived conceptuses and gilts carrying naturally-conceived conceptuses, but secretion of ENPP2 protein into the uterine lumen did decrease significantly in pigs with SCNT-derived conceptuses. These results indicate that expression and secretion of ENPP2, which are critical for appropriate LPA production and successful pregnancy, are dysregulated in the uterine endometrium of pigs carrying SCNT-derived conceptuses.
authors: Division of Biological Science and Technology, Institute of Biomaterials, and IPAID, Yonsei University, Wonju, 220-710, Korea.
authors: Heewon
authors: H
authors: Seo
authors: Division of Biological Science and Technology, Institute of Biomaterials, and IPAID, Yonsei University, Wonju, 220-710, Korea.
authors: Yohan
authors: Y
authors: Choi
authors: Division of Biological Science and Technology, Institute of Biomaterials, and IPAID, Yonsei University, Wonju, 220-710, Korea.
authors: Inkyu
authors: I
authors: Yu
authors: Division of Biological Science and Technology, Institute of Biomaterials, and IPAID, Yonsei University, Wonju, 220-710, Korea.
authors: Jangsoo
authors: J
authors: Shim
authors: Division of Biological Science and Technology, Institute of Biomaterials, and IPAID, Yonsei University, Wonju, 220-710, Korea.
authors: Chang-Kyu
authors: CK
authors: Lee
authors: Division of Biological Science and Technology, Institute of Biomaterials, and IPAID, Yonsei University, Wonju, 220-710, Korea.
authors: Sang-Hwan
authors: SH
authors: Hyun
authors: Division of Biological Science and Technology, Institute of Biomaterials, and IPAID, Yonsei University, Wonju, 220-710, Korea.
authors: Eunsong
authors: E
authors: Lee
authors: Division of Biological Science and Technology, Institute of Biomaterials, and IPAID, Yonsei University, Wonju, 220-710, Korea.
authors: Hakhyun
authors: H
authors: Ka
keywords: ENPP2; LPA; Pig; Pregnancy; SCNT