title: Transglutaminase-induced crosslinking of gelatin-calcium carbonate composite films.
abstract: The effects of transglutaminase (TGase) on the rheological profiles and interactions of gelatin-calcium carbonate solutions were studied. In addition, mechanical properties, water vapour permeability and microstructures of gelatin-calcium carbonate films were also investigated and compared. Fluorescence data suggested that the interaction of TGase and gelation-calcium carbonate belonged to a static quenching mechanism, and merely one binding site between TGase and gelatin-calcium carbonate was identified. Moreover, differential scanning calorimetry (DSC), the mechanical properties and the water vapour permeability studies revealed that TGase favoured the strong intramolecular polymerisation of the peptides in gelatin. The microstructures of the surfaces and cross sections in gelatin-calcium carbonate films were shown by scanning electron microscope (SEM) micrographs. The results of the fourier transform infrared spectroscopy (FTIR) indicated that TGase caused conformational changes in the proteins films. Therefore, TGase successfully facilitated the formation of gelatin-calcium carbonate composite films.
authors: College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
authors: Yuemeng
authors: Y
authors: Wang
authors: College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: laj@tust.edu.cn.
authors: Anjun
authors: A
authors: Liu
authors: Department of Biosystems Engineering and Soil Science, University of Tennessee, 2506 E.J. Chapman Drive, Knoxville, TN 37996-4531, USA. Electronic address: ran.ye.email@gmail.com.
authors: Ran
authors: R
authors: Ye
authors: College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
authors: Wenhang
authors: W
authors: Wang
authors: College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
authors: Xin
authors: X
authors: Li
mesh_terms: D002119:Calcium Carbonate / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D011503:Transglutaminases / Q000737:chemistry*
chemical_list: C015233:gelatin film; D005780:Gelatin; D011503:Transglutaminases; D002119:Calcium Carbonate
keywords: Calcium carbonate; Composite films; Gelatin; Transglutaminase