title: A new method for rapid construction of a Pseudomonas sp. HF-1 bioaugmented system: accelerating acylated homoserine lactones secretion by pH regulation.
abstract: Pseudomonas sp. HF-1 bioaugmented systems were operated to treat tobacco wastewater under pH 5.5 for three cycles and pH 8.0 for the rest, which was suitable for HF-1 biofilm formation. The results showed that, under pH control, the contents of 3-oxo-C6-HSL, C6-HSL and 3-oxo-C8-HSL were significantly higher than HF-1 thresholds for biofilm formation. Compared with non-pH controlled reactors, HF-1 showed greater colonization in pH controlled reactors, primarily owing to the high extracellular polymeric substances secretion induced by quorum sensing. Accordingly, high indigenous community activity and granular sludge were observed. Sludge granulation occurred from the seventh cycle, and the average diameter was greater than 400 μm. These systems were also highly efficient with nearly 100% nicotine degradation and 60% total organic carbon removal. Overall, the results indicate that pH regulation is a new and feasible method for acceleration of releasing of auto-inducers, which is beneficial to construction of HF-1 bioaugmented systems.
authors: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
authors: Mei-Zhen
authors: MZ
authors: Wang
authors: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
authors: Xin
authors: X
authors: Zheng
authors: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
authors: Kun
authors: K
authors: Zhang
authors: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
authors: Yang-Cheng
authors: YC
authors: Ding
authors: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
authors: Hong-Zhen
authors: HZ
authors: He
authors: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
authors: Dong-Sheng
authors: DS
authors: Shen
authors: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China. Electronic address: fenghuajun@mail.zjgsu.edu.cn.
authors: Hua-Jun
authors: HJ
authors: Feng
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development; D001704:Biopolymers / Q000032:analysis; D019149:Bioreactors / Q000382:microbiology; D052918:Environmental Restoration and Remediation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010088:Oxidoreductases / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism* / Q000502:physiology; D012722:Sewage / Q000382:microbiology
chemical_list: D054742:Acyl-Butyrolactones; D001704:Biopolymers; D012722:Sewage; D010088:Oxidoreductases
keywords: Bioaugmentation; Granulation; Pseudomonas sp. HF-1; Quorum sensing; pH regulation