title: Genetic traceability of black pig meats using microsatellite markers.
abstract: Pork from Jeju black pig (population J) and Berkshire (population B) has a unique market share in Korea because of their high meat quality. Due to the high demand of this pork, traceability of the pork to its origin is becoming an important part of the consumer demand. To examine the feasibility of such a system, we aim to provide basic genetic information of the two black pig populations and assess the possibility of genetically distinguishing between the two breeds. Muscle samples were collected from slaughter houses in Jeju Island and Namwon, Chonbuk province, Korea, for populations J and B, respectively. In total 800 Jeju black pigs and 351 Berkshires were genotyped at thirteen microsatellite (MS) markers. Analyses on the genetic diversity of the two populations were carried out in the programs MS toolkit and FSTAT. The population structure of the two breeds was determined by a Bayesian clustering method implemented in structure and by a phylogenetic analysis in Phylip. Population J exhibited higher mean number of alleles, expected heterozygosity and observed heterozygosity value, and polymorphism information content, compared to population B. The FIS values of population J and population B were 0.03 and -0.005, respectively, indicating that little or no inbreeding has occurred. In addition, genetic structure analysis revealed the possibility of gene flow from population B to population J. The expected probability of identify value of the 13 MS markers was 9.87×10(-14) in population J, 3.17×10(-9) in population B, and 1.03×10(-12) in the two populations. The results of this study are useful in distinguishing between the two black pig breeds and can be used as a foundation for further development of DNA markers.
authors: Genomic Informatics Center, Hankyong National University, Anseong 456-649, Korea.
authors: Jae-Don
authors: JD
authors: Oh
authors: Genomic Informatics Center, Hankyong National University, Anseong 456-649, Korea.
authors: Ki-Duk
authors: KD
authors: Song
authors: Genomic Informatics Center, Hankyong National University, Anseong 456-649, Korea.
authors: Joo-Hee
authors: JH
authors: Seo
authors: Genomic Informatics Center, Hankyong National University, Anseong 456-649, Korea.
authors: Duk-Kyung
authors: DK
authors: Kim
authors: Genomic Informatics Center, Hankyong National University, Anseong 456-649, Korea.
authors: Sung-Hoon
authors: SH
authors: Kim
authors: Department of Animal Science and Technology, Sunchon National University, Sunchon 540-742, Korea .
authors: Kang-Seok
authors: KS
authors: Seo
authors: Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Korea .
authors: Hyun-Tae
authors: HT
authors: Lim
authors: Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Korea .
authors: Jae-Bong
authors: JB
authors: Lee
authors: Dasan Pig Breeding Co., Namwon 590-831, Korea .
authors: Hwa-Chun
authors: HC
authors: Park
authors: Major of Animal Science and Biotechnology, Jeju National University, Jeju 690-756, Korea .
authors: Youn-Chul
authors: YC
authors: Ryu
authors: Major of Animal Science and Biotechnology, Jeju National University, Jeju 690-756, Korea .
authors: Min-Soo
authors: MS
authors: Kang
authors: C&K Genomics, Seoul 151-919, Korea .
authors: Seoae
authors: S
authors: Cho
authors: Genomic Informatics Center, Hankyong National University, Anseong 456-649, Korea ; Department of Animal Science, Iowa State University, Ames, IA 50011, USA .
authors: Eui-Soo
authors: ES
authors: Kim
authors: Department of Animal Biotechnology, Chonbuk National University, Jeonju 561-756, Korea .
authors: Ho-Sung
authors: HS
authors: Choe
authors: Genomic Informatics Center, Hankyong National University, Anseong 456-649, Korea.
authors: Hong-Sik
authors: HS
authors: Kong
authors: Genomic Informatics Center, Hankyong National University, Anseong 456-649, Korea.
authors: Hak-Kyo
authors: HK
authors: Lee
keywords: Black Pig Populations; Genetic Traceability; Heterozygosity; Microsatellite Markers; Probability of Identity