title: Substantial Effect of Melanin Influencing Factors on In vitro Melanogenesis in Muzzle Melanocytes of Differently Colored Hanwoo.
abstract: The present study was designed to investigate the effect of α-melanocyte-stimulating hormone (α-MSH), nitric oxide (NO) and L-cysteine on melanin production and expression of related genes MC1R, Tyr, Tyrp-1 and Tyrp-2 in muzzle melanocytes of differently colored three native Hanwoo cattle. Muzzle samples were taken from black, brindle and brown Hanwoo and purified melanocytes were cultured with α-MSH, nitric oxide and L-cysteine at 100 nM, 50 µM and 0.07 mg/ml of media respectively. The amounts of total melanin, eumelanin and mRNA expression at Tyr, Tyrp-1, Tyrp-2 and MC1R levels were quantified. α-MSH and nitric oxide significantly increased (p<0.05) the amount of total melanin in black and brindle whereas eumelanin production in brown Hanwoo muzzle melanocytes. On the contrary, L-cysteine greatly (p<0.05) depressed the eumelanin production in black color but increased in brown. Simultaneously, up regulation of Tyr by nitric oxide and α-MSH and down regulation of Tyr, Tyrp-2 and MC1R genes by L-cysteine were observed in muzzle melanocytes of all three phenotypes. The results of this study revealed nitric oxide and α-MSH contribute hyper-pigmentation by enhancing eumelanogenesis whereas L-cysteine contributes to pheomelanin production in different colored Hanwoo muzzle melanocytes.
authors: Department of Food and Nutrition, Nambu University, Gwangju 506-706, Korea .
authors: Touseef
authors: T
authors: Amna
authors: Department of Food and Nutrition, Nambu University, Gwangju 506-706, Korea .
authors: Kyoung Mi
authors: KM
authors: Park
authors: Department of Food and Nutrition, Nambu University, Gwangju 506-706, Korea .
authors: In-Kyung
authors: IK
authors: Cho
authors: National Institute of Animal Science, RDA, Cheonan 330-801, Korea .
authors: Tae Jeong
authors: TJ
authors: Choi
authors: National Institute of Animal Science, RDA, Cheonan 330-801, Korea .
authors: Seung Soo
authors: SS
authors: Lee
authors: Department of Animal Science and Technology, Suncheon National University, Suncheon, 540-742, Korea .
authors: Kang-Seok
authors: KS
authors: Seo
authors: Department of Food and Nutrition, Nambu University, Gwangju 506-706, Korea .
authors: Inho
authors: I
authors: Hwang
keywords: Cysteine; Hanwoo Muzzle; Melanocytes; Melanogenesis; Nitric Oxide