title: Antagonistic and synergistic effects of light irradiation on the effects of copper on Chlamydomonas reinhardtii.
abstract: The present study showed the important role of light intensity and spectral composition on Cu uptake and effects on green alga Chlamydomonas reinhardtii. High-intenisty light (HL) increased cellular Cu concentrations, but mitigated the Cu-induced decrease in chlorophyll fluorescence, oxidative stress and lipid peroxidation at high Cu concentrations, indicating that Cu and HL interact in an antagonistic manner. HL up-regulated the transcription of genes involved in the antioxidant response in C. reinhardtii and thus reduced the oxidative stress upon exposure to Cu and HL. Combined exposure to Cu and UVBR resulted in an increase of cellular Cu contents and caused severe oxidative damage to the cells. The observed effects were higher than the sum of the effects corresponding to exposure to UVBR or Cu alone suggesting a synergistic interaction.
authors: Environmental Biogeochemistry and Ecotoxicology, Institute F.-A. Forel, Earth and Environmental Sciences, Faculty of Sciences, University of Geneva, 10, Route de Suisse, CH-1290 Versoix, Switzerland.
authors: Giulia
authors: G
authors: Cheloni
authors: Environmental Biogeochemistry and Ecotoxicology, Institute F.-A. Forel, Earth and Environmental Sciences, Faculty of Sciences, University of Geneva, 10, Route de Suisse, CH-1290 Versoix, Switzerland.
authors: Claudia
authors: C
authors: Cosio
authors: Environmental Biogeochemistry and Ecotoxicology, Institute F.-A. Forel, Earth and Environmental Sciences, Faculty of Sciences, University of Geneva, 10, Route de Suisse, CH-1290 Versoix, Switzerland. Electronic address: vera.slaveykova@unige.ch.
authors: Vera I
authors: VI
authors: Slaveykova
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000528:radiation effects*; D003300:Copper / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008027:Light / Q000009:adverse effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D014874:Water Pollutants, Chemical; D003300:Copper
keywords: Combined stress; Copper; Oxidative stress; PCR; ROS; UVB irradiation