title: Rapid metabolic discrimination and prediction of dioscin content from African yam tubers using Fourier transform-infrared spectroscopy combined with multivariate analysis.
abstract: To determine whether or not FT-IR spectroscopy could be used for taxonomic and metabolic discrimination of African yam lines, tuber samples from African and Asian yam species were subjected to FT-IR. Most remarkable spectral differences between African and Asian yams were found in the 1750-1700 cm(-1) region, polysaccharide (1200-900 cm(-1)) and protein/amide I and II (1700-1500 cm(-1)) regions of FT-IR spectra. A hierarchical dendrogram based on partial least square-discriminant analysis (PLS-DA) of FT-IR data from 7 African yam species show phylogenetic relationship. In addition, the content of dioscin, a steroidal saponin found in yam tuber, was predicted using a PLS regression model with regression coefficient R(2)=0.7208 indicated that prediction model had average accuracy. Thus, considering these results we suggest that FT-IR combined with multivariate analysis could be applied as a novel tool for metabolic evaluation and high-throughput screening of African yam lines with higher content of dioscin.
authors: Microbiological Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon 305-806, South Korea; Western Seoul Center, Korea Basic Science Institute, 150 Bugahyeon-ro, Seodamun-gu, Seoul 120-140,South Korea.
authors: Yong-Kook
authors: YK
authors: Kwon
authors: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon 305-806, South Korea.
authors: Eun Yee
authors: EY
authors: Jie
authors: International Institute of Tropical Agriculture, c/o IITA Ltd., Carolyn House, 26 Dingwall Road, Croydon CR9 3EE, United Kingdom.
authors: Alieu
authors: A
authors: Sartie
authors: School of Life Science and Bioengineering, The Nelson Mandela African Institute of Science and Technology (NM-AIST), P.O. Box 447, Arusha, Tanzania. Electronic address: dj.kim@nm-aistac.tz.
authors: Dong Jin
authors: DJ
authors: Kim
authors: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon 305-806, South Korea.
authors: Jang Ryol
authors: JR
authors: Liu
authors: School of Ecological & Environmental System, Kyungpook National University, Sangju 742-711, South Korea.
authors: Byung Whan
authors: BW
authors: Min
authors: Microbiological Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon 305-806, South Korea. Electronic address: kimsw@kribb.re.kr.
authors: Suk Weon
authors: SW
authors: Kim
mesh_terms: D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000737:chemistry; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: C019357:dioscin; D004144:Diosgenin
keywords: African yam tuber; Dioscin; Fourier transform-infrared spectroscopy; Multivariate analysis; PLS (partial least square) regression