title: The glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH) is regulated by myeloid zinc finger 1 (MZF-1) and is induced by calcitriol.
abstract: Recently, new tissue-specific functions for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) have been discovered, aside from its archetypal function in glycolysis. This casts doubt on the legitimacy of using GAPDH as a normalization control for gene expression analysis. We report the binding of the myeloid zinc finger-1 (MZF-1) transcription factor to the human GAPDH promoter. Furthermore, we show that up-regulation of MZF-1 by 1,25-dihydroxyvitamin D3 (calcitriol) induces GAPDH in HS-5 stromal fibroblasts, while knockdown of MZF1 by shRNA leads to a concomitant reduction in GAPDH expression. This argues that MZF-1 regulates GAPDH, indicating a role for GAPDH in calcitriol-mediated signaling.
authors: Department of Sciences, John Jay College, The City University of New York, United States; Albert Einstein College of Medicine, Yeshiva University, United States.
authors: Richard T
authors: RT
authors: Piszczatowski
authors: Department of Science, Borough of Manhattan Community College, The City University of New York, New York, NY 10019, United States.
authors: Brian J
authors: BJ
authors: Rafferty
authors: Department of Sciences, John Jay College, The City University of New York, United States.
authors: Andre
authors: A
authors: Rozado
authors: Department of Sciences, John Jay College, The City University of New York, United States.
authors: Szilvia
authors: S
authors: Tobak
authors: Department of Sciences, John Jay College, The City University of New York, United States. Electronic address: nlents@jjay.cuny.edu.
authors: Nathan H
authors: NH
authors: Lents
mesh_terms: D001483:Base Sequence; D002117:Calcitriol / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering; D015398:Signal Transduction; D014212:Tretinoin / Q000378:metabolism / Q000494:pharmacology
chemical_list: D051741:Kruppel-Like Transcription Factors; C085969:MZF1 protein, human; D034741:RNA, Small Interfering; D014212:Tretinoin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D002117:Calcitriol
keywords: Calcitriol; GAPDH; Glycolysis; MZF1; Vitamin D