title: Effects of mTOR-STAT3 on the migration and invasion abilities of hepatoma cell and mTOR-STAT3 expression in liver cancer.
abstract: OBJECTIVE
  To investigate the effects of mTOR-STAT3 pathway on the invasion and migration of hepatoma cell.
  
  
  METHODS
  mTOR and STAT3 expression in the hepatocellular carcinoma cell line HepG2 and normal liver cell line L02 were detected by reverse transcription PCR (RT-PCR) and western blotting. The migration and invasion abilities of cells and expression of STAT3 were detected by scratch adhesion test and transwell migration assays, after siRNA transfection blocking mTOR expression of HepG2 cells.
  
  
  RESULTS
  The HepG2 cells expression is higher compared with normal cells L02 expression. Western blotting assay showed the mTOR expression was blocked, while STAT3 expression was also decreased, after the siRNA transfection of HepG2 cells. The migration (scratch adhesion test) and invasion (transwell assays) abilities of HepG2 cells which the mTOR expression was blocked by siRNA interference were significantly decreased (P<0.05).
  
  
  CONCLUSIONS
  mTORSTAT3 expression in hepatoma cells HepG2 was significantly higher than that in normal liver cells. mTOR blocking can reduce the expression of STAT3, which is also closely related to the invasion and metastasis of liver cancer cells.
authors: Department of Pathology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, China. Electronic address: puxia_79@163.com.
authors: Xia
authors: X
authors: Pu
authors: Department of Pathology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, China.
authors: Qing-Xi
authors: QX
authors: Guo
authors: Department of Pathology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, China.
authors: Han-An
authors: HA
authors: Long
authors: Department of Pathology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, China.
authors: Cheng-Wan
authors: CW
authors: Yang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D050796:STAT3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases
keywords: Invasion; Liver cancer; Metastasis; mTOR/STAT3