title: Multiple GO-SELEX for efficient screening of flexible aptamers.
abstract: We describe a simple, high-speed, high-throughput aptamer screening for a group of small molecules using graphene oxide (simple Multi-GO-SELEX) without immobilizing targets. The affinities of ten different ssDNA aptamers successfully obtained for three pesticides were in the range of 10-100 nM. Besides a specific aptamer for each target, we found a couple of flexible multi-target aptamers, which can bind with 2 or 3 different molecules. These flexible aptamers developed for binding with a mixture of targets are not only significant for the rapid screening of a group of small molecules but also offer great promise for aptamer-based biosensor applications.
authors: School of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-Gu, Seoul 136-713, Republic of Korea. mbgu@korea.ac.kr.
authors: Van-Thuan
authors: VT
authors: Nguyen
authors: Young Seop
authors: YS
authors: Kwon
authors: Jae Hoon
authors: JH
authors: Kim
authors: Man Bock
authors: MB
authors: Gu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D003124:Colorimetry; D004277:DNA, Single-Stranded / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D010575:Pesticides / Q000032:analysis; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D010087:Oxides; D010575:Pesticides; D006046:Gold; D006108:Graphite