title: Characterization and antitumor activity of a polysaccharide from Sarcodia ceylonensis.
abstract: A water-soluble polysaccharide from Sarcodia ceylonensis was obtained by using the method of water-extraction and ethanol-precipitation. The polysaccharide was further purified by chromatography on AB-8 and ADS-7 columns, yielding a pure polysaccharide termed SCP-60. The molecular weight (Mw) of SCP-60 was calculated to be 50.0 kDa, based on the calibration curve obtained with a series of Dextran T standards. The results of FT-IR indicated that the polysaccharide contains the α-configuration of sugar units. GC-MS analysis revealed that SCP-60 was mainly composed of galactose and glucose. NMR spectroscopy revealed SCP-60 had the backbone consisting of → 6)-α-Manp-(1 →, α-D-Glcp-(1 →, → 6)-α-D-Glcp-(1 → and → 6)-α-Galp-(1 →. In order to evaluate the antitumor activity in vivo of the polysaccharide, a sarcoma 180 model was used. The results showed SCP-60 had strong antitumor ability, meanwhile, SCP-60 at a high dose (100 mg/kg) could significantly increase the thymic and splenic indices of S180 mice, and strongly promote the secretion of IL-2, TNF-α and IFN-γ, increase the SOD activities and reduce the concentrations of MDA in blood. Therefore the polysaccharide SCP-60 should be explored as a novel potential antitumor drug.
authors: Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China.
authors: Yijun
authors: Y
authors: Fan
authors: Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China.
authors: Mengchuan
authors: M
authors: Lin
authors: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors: Aoshuang
authors: A
authors: Luo
authors: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors: Ze
authors: Z
authors: Chun
authors: Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China. aoxueluo@sicau.edu.cn.
authors: Aoxue
authors: A
authors: Luo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D053147:Plant Exudates / Q000737:chemistry / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000461:Rhodophyta / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D016207:Cytokines; D053147:Plant Exudates; D011134:Polysaccharides; D008315:Malondialdehyde; D013482:Superoxide Dismutase