title: Toxicological assessment of trace β-diketone antibiotic mixtures on zebrafish (Danio rerio) by proteomic analysis.
abstract: β-Diketone antibiotics (DKAs) can produce chronic toxicity in aquatic ecosystems due to their pseudo-persistent in the environment. In this study, after long-term DKA exposure to zebrafish (Danio rerio), 47 protein spots had greater than 2-fold differential expression as compared to the control; there were 26 positive proteins with 14 up-regulated and 12 down-regulated. The main functions of the differentially expressed proteins were related to signal transduction mechanisms and the cytoskeleton. Of the 26 target genes, 11 genes were consistent between their transcriptional and translational levels. Low dose DKA exposure (4.69 and 9.38 mg/L) stimulated spontaneous movement in zebrafish. Changes in both creatine kinase activity and creatine concentration showed a similar trend to zebrafish activity. There was no obvious change in SV-BA after DKA exposure, while a reduction of heart rate was concomitant with increasing DKA concentrations. DKAs also induced severe histopathological changes in zebrafish heart tissue, such as dissolution of cristae and vacuolation of mitochondria. These results demonstrated that trace-level DKA exposure affects a variety of cellular and biological processes in zebrafish.
authors: Institute of Wenzhou Applied Technology for Environmental Research, Wenzhou Medical University, Wenzhou, China.
authors: Xiaohan
authors: X
authors: Yin
authors: Key Laboratory of Wenzhou Marine Biology, College of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors: Huili
authors: H
authors: Wang
authors: Institute of Wenzhou Applied Technology for Environmental Research, Wenzhou Medical University, Wenzhou, China.
authors: Yuna
authors: Y
authors: Zhang
authors: Institute of Wenzhou Applied Technology for Environmental Research, Wenzhou Medical University, Wenzhou, China.
authors: Randy A
authors: RA
authors: Dahlgren
authors: Key Laboratory of Wenzhou Marine Biology, College of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors: Hongqin
authors: H
authors: Zhang
authors: Key Laboratory of Wenzhou Marine Biology, College of Life Sciences, Wenzhou Medical University, Wenzhou, China.
authors: Mengru
authors: M
authors: Shi
authors: Institute of Wenzhou Applied Technology for Environmental Research, Wenzhou Medical University, Wenzhou, China.
authors: Ming
authors: M
authors: Gao
authors: Institute of Wenzhou Applied Technology for Environmental Research, Wenzhou Medical University, Wenzhou, China.
authors: Xuedong
authors: X
authors: Wang
mesh_terms: D000818:Animals; D017753:Ecosystem; D024841:Fluoroquinolones / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D040901:Proteomics; D013754:Tetracyclines / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity; D015027:Zebrafish / Q000235:genetics; D029961:Zebrafish Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D024841:Fluoroquinolones; D013754:Tetracyclines; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins