title: [Measurement of genital mycoplasma concentration by using a microbiological and molecular-biological methods].
abstract: OBJECTIVE
  Establishment of ratios that would allow to execute recalculation of mycoplasma concentration from CFU/ml and/or CCU/ml into units obtained during PCR analysis--geq/ml.
  
  
  METHODS
  Pure cultures of Mycoplasma hominis, Ureaplasma parvum and Ureaplasma urealyticum were studied by cultural and molecular-biological methods with quantitative evaluation. Studies of initial cultures as well as series of 10-fold dilutions were carried out. 32 experiments in total were carried out.
  
  
  RESULTS
  Ratio between geq/ml and CFU/ml for M. hominis was 3.5; geq/ ml and CCU/ml ratio--4.4. Ratio between geq/ml and CCU/ml for U. parvum was 7.1; for U. urealyticum--11.2.
  
  
  CONCLUSIONS
  Ratios between indexes obtained during quantitative study of pure genital micoplasma cultures by using 2 methods were established.
authors: T A
authors: TA
authors: Ivanova
authors: A E
authors: AE
authors: Gushchin
authors: N A
authors: NA
authors: Gamova
authors: O I
authors: OI
authors: Barkhatova
authors: I V
authors: IV
authors: Rakovskaia
mesh_terms: D015169:Colony Count, Microbial / Q000592:standards* / Q000706:statistics & numerical data; D003470:Culture Media; D006801:Humans; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000382:microbiology; D019535:Mycoplasma hominis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000592:standards* / Q000706:statistics & numerical data; D012044:Regression Analysis; D014509:Ureaplasma / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016869:Ureaplasma Infections / Q000175:diagnosis / Q000382:microbiology; D016990:Ureaplasma urealyticum / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D014566:Urogenital System / Q000382:microbiology
chemical_list: D003470:Culture Media