title: Simultaneous determination of binding constants for multiple carbohydrate hosts in complex mixtures.
abstract: We describe a simple method for the simultaneous determination of association constants for a guest binding to seven different hosts in a mixture of more than 20 different oligosaccharides. If the binding parameters are known for one component in the mixture, a single NMR titration suffices to determine binding constants for all other detectable and resolvable hosts. With the use of high-resolution (1)H-(13)C HSQC experiments, complexes of amphiphiles with more than 10 different maltooligosaccharides can be resolved. Hereby, the binding capabilities of a set of structurally related hosts can be quantitatively studied to systematically explore noncovalent interactions without the need to isolate each host.
authors: Department of Chemistry, Technical University of Denmark, Kemitorvet , Building 201, Kgs Lyngby 2800, Denmark.
authors: Sebastian
authors: S
authors: Meier
authors: Sophie R
authors: SR
authors: Beeren
mesh_terms: D003505:Cyclodextrins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
chemical_list: D003505:Cyclodextrins