title: Bactericidal activities of GM flax seedcake extract on pathogenic bacteria clinical strains.
abstract: BACKGROUND
  The antibiotic resistance of pathogenic microorganisms is a worldwide problem. Each year several million people across the world acquire infections with bacteria that are antibiotic-resistant, which is costly in terms of human health. New antibiotics are extremely needed to overcome the current resistance problem.
  
  
  RESULTS
  Transgenic flax plants overproducing compounds from phenylpropanoid pathway accumulate phenolic derivatives of potential antioxidative, and thus, antimicrobial activity. Alkali hydrolyzed seedcake extract containing coumaric acid, ferulic acid, caffeic acid, and lignan in high quantities was used as an assayed against pathogenic bacteria (commonly used model organisms and clinical strains). It was shown that the extract components had antibacterial activity, which might be useful as a prophylactic against bacterial infection. Bacteria topoisomerase II (gyrase) inhibition and genomic DNA disintegration are suggested to be the main reason for rendering antibacterial action.
  
  
  CONCLUSIONS
  The data obtained strongly suggest that the seedcake extract preparation is a suitable candidate for antimicrobial action with a broad spectrum and partial selectivity. Such preparation can be applied in cases where there is a risk of multibacterial infection and excellent answer on global increase in multidrug resistance in pathogenic bacteria.
authors: Faculty of Biotechnology, Wrocław University, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland. mzuk@ibmb.uni.wroc.pl.
authors: Magdalena
authors: M
authors: Zuk
authors: Agata
authors: A
authors: Dorotkiewicz-Jach
authors: Zuzanna
authors: Z
authors: Drulis-Kawa
authors: Malgorzata
authors: M
authors: Arendt
authors: Anna
authors: A
authors: Kulma
authors: Jan
authors: J
authors: Szopa
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004250:DNA Topoisomerases, Type II / Q000737:chemistry / Q000378:metabolism; D019597:Flax / Q000737:chemistry* / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D012867:Skin / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D004250:DNA Topoisomerases, Type II