title: Effect of oxygenated polyethylene glycol decorated hemoglobin on microvascular diameter and functional capillary density in the transgenic mouse model of sickle cell anemia.
abstract: Malemide polyethylene glycol-conjugated Hb (MP4OX, Sangart Inc.), a high-affinity low-concentration acellular hemoglobin (P50 = 5 mmHg, 4.3 g/dl) solution, has been shown to optimize microvascular perfusion and target oxygen delivery to anoxic tissue. Microvascular perfusion during an acute hypoxic challenge in a transgenic anemic sickle cell disease mouse model was studied with MP4OX and saline. Arterioles were dilated in both groups. Functional capillary density (FCD) was maintained at a higher level with MP4OX. In conclusion, MP4OX treatment reduced the hypoxia-mediated decline in FCD, an effect in part due to higher arterial pressure resulting in increased microvascular perfusion pressures.
authors: Department of Bioengineering, University of California , San Diego, La Jolla, CA , USA.
authors: Amy G
authors: AG
authors: Tsai
authors: Pedro
authors: P
authors: Cabrales
authors: Mark A
authors: MA
authors: Young
authors: Robert M
authors: RM
authors: Winslow
authors: Marcos
authors: M
authors: Intaglietta
mesh_terms: D000208:Acute Disease; D000755:Anemia, Sickle Cell / Q000097:blood / Q000473:pathology / Q000628:therapy*; D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry / Q000494:pharmacology*; D002196:Capillaries / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry; D004195:Disease Models, Animal; D005260:Female; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology*; D006579:Heterozygote; D000860:Hypoxia / Q000097:blood / Q000473:pathology / Q000628:therapy*; D008297:Male; D008301:Maleimides / Q000737:chemistry; D051379:Mice; D008822:Mice, Transgenic; D008833:Microcirculation / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001802:Blood Substitutes; D003432:Cross-Linking Reagents; D006454:Hemoglobins; D008301:Maleimides; C043592:maleimide; D011092:Polyethylene Glycols; D010100:Oxygen
keywords: cell-free hemoglobin; hypoxia; microcirculation; perfusion