title: Fabrication and structure analysis of poly(lactide-co-glycolic acid)/silk fibroin hybrid scaffold for wound dressing applications.
abstract: Silk fibroin (SF) and poly(lactide-co-glycolic acid) (PLGA) have been proved to be invaluable polymers in the field wound healing. This study aims at optimizing the electrospinning process of those polymers to make a hybrid membrane as a chronic wounds dressing. After characterizing the scaffolds, PLGA/SF (2:1), and PLGA scaffolds were selected for further study according to their superior tensile mechanical properties. The attachment and proliferation of mouse fibroblasts (L929) on scaffolds were measured using colorimetric assay and scanning electron microscopy. Furthermore, to evaluate the wound healing effect of the scaffolds in comparison with gauze and Comfeel(®) dressings, an excision wound model was conducted on diabetic rats. On the postoperative days of 3, 6, 9, 12, and 15, residual wound area was calculated using macroscopic data. In vitro results showed that the attachment and proliferation of L929 were significantly increased on PLGA/SF (2:1) hybrid scaffold. Animal study and histopathological evaluation outcomes confirmed the in vitro results as well. On day 15, the residual wound area in PLGA/SF (2:1) hybrid membrane group was significantly smaller than PLGA and control groups. This promising scaffold has the potential to be used for the upcoming development of wound dressings with or without biological drugs.
authors: Nanomedicine and Biomaterial Lab, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Sheida
authors: S
authors: Shahverdi
authors: Nanomedicine and Biomaterial Lab, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Nano Alvand Co., Avicenna Tech Park, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mirhamed
authors: M
authors: Hajimiri
authors: Nanomedicine and Biomaterial Lab, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohammad Amin
authors: MA
authors: Esfandiari
authors: Endocrinology and Metabolism Research Center (EMRC), Tehran University Medical Sciences, Tehran, Iran.
authors: Bagher
authors: B
authors: Larijani
authors: Nanomedicine and Biomaterial Lab, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Fatemeh
authors: F
authors: Atyabi
authors: Department of Pathology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Afsaneh
authors: A
authors: Rajabiani
authors: Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Ahmad Reza
authors: AR
authors: Dehpour
authors: Department of Textile Engineering, Amirkabir University of Technology, Tehran, Iran.
authors: Ali Akbar
authors: AA
authors: Gharehaghaji
authors: Nanomedicine and Biomaterial Lab, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: dinarvand@tums.ac.ir.
authors: Rassoul
authors: R
authors: Dinarvand
mesh_terms: D000818:Animals; D001458:Bandages; D002460:Cell Line; D003921:Diabetes Mellitus, Experimental; D005347:Fibroblasts; D005349:Fibroins / Q000008:administration & dosage / Q000737:chemistry*; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry*; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017207:Rats, Sprague-Dawley; D047011:Silk / Q000737:chemistry*; D054457:Tissue Scaffolds; D014945:Wound Healing
chemical_list: D047011:Silk; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005349:Fibroins
keywords: Diabetic wounds; Electrospun scaffold; PLGA; Silk fibroin; Wound dressing