title: Preparation and evaluation of bovine serum albumin immobilized chiral monolithic column for affinity capillary electrochromatography.
abstract: A system of capillary silica monolith with bovine serum albumin (BSA) functionalized through two approaches for affinity monolithic capillary electrochromatography (AMCEC) was developed. Covalent immobilization conditions for two different Schiff base methods, which employed 3-glycidopropyl trimethoxysilane (GPTS) and 3-aminopropyl trimethoxysilane (APTS) as starting materials, respectively, were investigated to obtain good and stable chiral separation. The BSA immobilized silica monoliths were evaluated in terms of morphology, electroosmotic flow, retention time, column efficiency and resolution of model compound (±)-tryptophan. The columns exhibited satisfactory run-to-run, column-to-column repeatability and maintained their enantioselectivity for more than 3months. Both developed methods can baseline separate tryptophan enantiomers, whereas shorter retention time, better column efficiency, and enantiomeric recognition between two pairs of drug enantiomers (pantoprazole and atenolol) were obtained by the GPTS method.
authors: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
authors: Tingting
authors: T
authors: Hong
authors: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
authors: Yan
authors: Y
authors: Zheng
authors: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
authors: Wenwen
authors: W
authors: Hu
authors: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China. Electronic address: jiyibing@msn.com.
authors: Yibing
authors: Y
authors: Ji
mesh_terms: D053801:Capillary Electrochromatography / Q000295:instrumentation*; D002846:Chromatography, Affinity / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D012710:Serum Albumin, Bovine
keywords: Affinity capillary electrochromatography; Bovine serum albumin; Capillary silica monolith; Enantiomer selectivity; Post-functionalization; Schiff base methods