title: Precise characterization method of antibody-conjugated magnetic nanoparticles for pathogen detection using stuffer-free multiplex ligation-dependent probe amplification.
abstract: Antibody-conjugated magnetic nanoparticles (Ab-MNPs) have potential in pathogen detection because they allow target cells to be easily separated from complex sample matrices. However, the sensitivity and specificity of pathogen capture by Ab-MNPs generally vary according to the types of MNPs, antibodies, and sample matrices, as well as preparation methods, including immobilization. Therefore, achieving a reproducible analysis utilizing Ab-MNPs as a pathogen detection method requires accurate characterization of Ab-MNP capture ability and standardization of all handling processes. In this study, we used high-resolution CE-single strand conformational polymorphism coupled with a stuffer-free multiplex ligation-dependent probe amplification system to characterize Ab-MNPs. The capture ability of Ab-MNPs targeting Salmonella enteritidis and nine pathogens, including S. enteritidis, was analyzed in phosphate buffer and milk. The effect of storage conditions on the stability of Ab-MNPs was also assessed. The results showed that the stuffer-free multiplex ligation-dependent probe amplification system has the potential to serve as a standard characterization method for Ab-MNPs. Moreover, the precise characterization of Ab-MNPs facilitated robust pathogen detection in various applications.
authors: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Korea.
authors: Boram
authors: B
authors: Chung
authors: Gi Won
authors: GW
authors: Shin
authors: Woong
authors: W
authors: Choi
authors: Jinmyoung
authors: J
authors: Joo
authors: Sangmin
authors: S
authors: Jeon
authors: Gyoo Yeol
authors: GY
authors: Jung
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods*; D019075:Electrophoresis, Capillary; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008460:Meat / Q000382:microbiology; D008892:Milk / Q000382:microbiology; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D058726:Nanoconjugates / Q000737:chemistry*; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D000906:Antibodies; D058185:Magnetite Nanoparticles; D058726:Nanoconjugates
keywords: Antibody-conjugated magnetic nanoparticles; Capillary electrophoresis-single-strand conformation polymorphism; Pathogen detection; Stuffer-free multiplex ligation-dependent probe amplification