title: Determination of the antiradical properties of olive oils using an electrochemical method based on DPPH radical.
abstract: The present work describes the development of an electrochemical method based on the use of 2,2'-diphenyl-1-picrylhidrazyl free radical (DPPH) for the determination of the antiradical properties of several olive oils. Differential pulse voltammetry was used as measuring technique while the electrochemical process was recorded at a platinum screen-printed working electrode. The decrease in 2,2'-diphenyl-1-picrylhidrazyl peak current intensity was measured at a specific potential value of +160 mV vs. screen-printed pseudo-reference electrode, in the presence of α-, δ- and γ-tocopherol and olive oil samples, respectively. The obtained results using differential pulse voltammetry, as detection technique for real samples analysis, showed a satisfactory agreement with those obtained by high-performance liquid chromatography coupled with fluorescence detection. The reported electrochemical method is rapid and easy to use, feasible and accessible to be used as an alternative to 2,2'-diphenyl-1-picrylhidrazyl spectrophotometric based method.
authors: Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences - Bucharest, 296 Splaiul Independentei, 060031 Bucharest, Romania.
authors: Ioana
authors: I
authors: Vasilescu
authors: Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences - Bucharest, 296 Splaiul Independentei, 060031 Bucharest, Romania. Electronic address: sandraeremia@gmail.com.
authors: Sandra A V
authors: SAV
authors: Eremia
authors: Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences - Bucharest, 296 Splaiul Independentei, 060031 Bucharest, Romania.
authors: Camelia
authors: C
authors: Albu
authors: National Institute for Research and Development in Microtechnology (IMT-Bucharest), 126A Erou Iancu Nicolae, 077190 Bucharest, Romania.
authors: Antonio
authors: A
authors: Radoi
authors: Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences - Bucharest, 296 Splaiul Independentei, 060031 Bucharest, Romania.
authors: Simona-Carmen
authors: SC
authors: Litescu
authors: Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences - Bucharest, 296 Splaiul Independentei, 060031 Bucharest, Romania.
authors: Gabriel-Lucian
authors: GL
authors: Radu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005609:Free Radicals; D000069463:Olive Oil / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D024505:Tocopherols / Q000737:chemistry*; D014810:Vitamin E / Q000737:chemistry*
chemical_list: D005609:Free Radicals; D000069463:Olive Oil; D010938:Plant Oils; D014810:Vitamin E; D024505:Tocopherols
keywords: 2,2′-Diphenyl-1-picrylhidrazyl; Differential pulse voltammetry; Olive oils; Tocopherols; Vitamin E