title: Fish viperin exerts a conserved antiviral function through RLR-triggered IFN signaling pathway.
abstract: Mammalian viperin is a typical interferon (IFN)-induced antiviral protein. Fish have viperin homologs; however, little is known about the expression regulation of fish viperins. In this study, we report the expression regulation and antiviral function of a fish viperin from crucian carp Carassius auratus during IFN response. Crucian carp viperin is induced at mRNA and protein levels by fish IFNs and IFN stimuli such as poly(I:C). Consistently, this gene promoter contains multiple transcription factor binding sites including IFN-stimulated response elements (ISRE) and IFN gamma activation sequences (GAS), and is activated by two types of fish IFNs and also by the intracellular and extracellular poly(I:C). Activation of crucian carp viperin promoter by the intracellular poly(I:C) is mediated by retinoic acid-inducing gene I (RIG-I)-like receptors (RLR)-triggered IFN signaling pathway, which is further verified by the findings that each signaling molecule of RLR pathway is able to induce the expression of crucian carp viperin at mRNA and protein levels. Finally, overexpression of crucian carp viperin in cultured fish cells confers significant protection against infection of grass carp reovirus (GCRV). These data suggest that similar to mammalian homologs, crucian carp viperin exerts a conserved function through RLR-triggered IFN signaling pathway.
authors: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China.
authors: Bing
authors: B
authors: Wang
authors: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: ybzhang@ihb.ac.cn.
authors: Yi-Bing
authors: YB
authors: Zhang
authors: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China.
authors: Ting-Kai
authors: TK
authors: Liu
authors: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China.
authors: Jun
authors: J
authors: Shi
authors: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China.
authors: Fan
authors: F
authors: Sun
authors: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: jfgui@ihb.ac.cn.
authors: Jian-Fang
authors: JF
authors: Gui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000276:immunology* / Q000821:virology*; D002478:Cells, Cultured; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000737:chemistry / Q000276:immunology*; D007113:Immunity, Innate; D050835:Interferon Regulatory Factors / Q000378:metabolism; D007372:Interferons / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D012327:RNA Virus Infections / Q000276:immunology / Q000662:veterinary*; D012087:Reoviridae / Q000502:physiology; D015398:Signal Transduction
chemical_list: D029941:Fish Proteins; D050835:Interferon Regulatory Factors; D007372:Interferons
keywords: Antiviral function; Carassius auratus; IFN inducible protein; Promoter analysis; RLR signaling pathway; Viperin