title: A fluorescence detection of D-penicillamine based on Cu(2+)-induced fluorescence quenching system of protein-stabilized gold nanoclusters.
abstract: In this contribution, a luminescent gold nanoclusters which were synthesized by bovine serum albumin as novel fluorescent probes were successfully utilized for the determination of D-penicillamine for the first time. Cupric ion was employed to quench the strong fluorescence of the gold nanoclusters, whereas the addition of D-penicillamine caused obvious restoration of fluorescence intensity of the Cu(2+)-gold nanoclusters system. Under optimum conditions, the increment in fluorescence intensity of Cu(2+)-gold nanoclusters system caused by D-penicillamine was linearly proportional to the concentration of D-penicillamine in the range of 2.0×10(-5)-2.39×10(-4) M. The detection limit for D-penicillamine was 5.4×10(-6) M. With the off-on fluorescence signal at 650 nm approaching the near-infrared region, the present sensor for D-penicillamine detection had high sensitivity and low spectral interference. Furthermore, the novel gold nanoclusters-based fluorescent sensor has been applied to the determination of D-penicillamine in real biological samples with satisfactory results.
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Peng
authors: P
authors: Wang
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Bang Lin
authors: BL
authors: Li
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Nian Bing
authors: NB
authors: Li
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: luohq@swu.edu.cn.
authors: Hong Qun
authors: HQ
authors: Luo
mesh_terms: D000818:Animals; D002021:Buffers; D002138:Calibration; D002417:Cattle; D003300:Copper / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007477:Ions; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D010396:Penicillamine / Q000097:blood*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D002021:Buffers; D007477:Ions; D012710:Serum Albumin, Bovine; D006046:Gold; D003300:Copper; D010396:Penicillamine
keywords: Cupric ion; Fluorescence; Gold nanoclusters; Turn-on sensor; d-Penicillamine