title: Differential nuclear shape dynamics of invasive andnon-invasive breast cancer cells are associated with actin cytoskeleton organization and stability.
abstract: Cancer cells often exhibit characteristic aberrations in their nuclear architecture, which are indicative of their malignant potential. In this study, we have examined the nuclear and cytoskeletal composition, attachment configuration dynamics, and osmotic or drug treatment response of invasive (Hs578T and MDA-MB-231) and non-invasive (MCF-10A and MCF-7) breast cancer cell lines. Unlike MCF-10A and MCF-7, Hs578T and MDA-MB-231 cells showed extensive nuclear elasticity and deformability and displayed distinct kinetic profiles during substrate attachment. The nuclear shape of MCF-10A and MCF-7 cells remained almost unaffected upon detachment, hyperosmotic shock, or cytoskeleton depolymerization, while Hs578T and MDA-MB-231 revealed dramatic nuclear contour malformations following actin reorganization.
authors: Department of Biochemistry, School of Medicine, University of Crete, Heraklion 71003, Greece.
authors: Rena
authors: R
authors: Chiotaki
authors: Hara
authors: H
authors: Polioudaki
authors: Panayiotis A
authors: PA
authors: Theodoropoulos
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D002467:Cell Nucleus / Q000473:pathology; D053144:Cell Nucleus Shape; D053145:Cell Nucleus Size; D005260:Female; D006706:Homeostasis; D006801:Humans; D009361:Neoplasm Invasiveness; D055550:Protein Stability
keywords: actin cytoskeleton organization; adhérence cellulaire; cell attachment; cellules de cancer du sein invasives et non-invasives; cytoskeleton; cytosquelette; forme et déformabilité du noyau; invasive and non-invasive breast cancer cells; nuclear shape and deformability; organisation du cytosquelette d’actine