title: Development and validation of an ultra-performance liquid chromatography method for simultaneous analysis of 20 antihistaminics in dietary supplements.
abstract: The purpose of this study was to develop and validate an ultra-performance liquid chromatography method for simultaneous analysis of 20 antihistamines (illegal additives) in dietary supplements. The limits of detection and quantitation of the method ranged from 1.5 to 2.5 µg/mL and from 20.0 to 50.0 µg/mL, respectively. The determination coefficient was >0.999, precisions were 0.2-5.1% (intra-day) and 0.1-8.8% (inter-day), and accuracies were 84.5-111.2% (intra-day) and 91.9-112.0% (inter-day). The mean recoveries of 20 targeted compounds from dietary supplements ranged from 75.4 to 119.3%. The relative standard deviations were <6.6% and complied with established international guidelines. The relative standard deviation of stability was <0.8%. Fifty-two commercially available dietary supplements were evaluated using this method, and were found to have none of the 20 antihistamines in significant abundance.
authors: Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, 187 Osongsaengmyeong2-ro, Osongeup, Cheongwon-gun, Chungcheongbuk-do, 363-700, Republic of Korea.
authors: Jung Yeon
authors: JY
authors: Kim
authors: Jung-Ah
authors: JA
authors: Do
authors: Ji Yeon
authors: JY
authors: Choi
authors: Sooyeul
authors: S
authors: Cho
authors: Woo-Seong
authors: WS
authors: Kim
authors: Chang-Yong
authors: CY
authors: Yoon
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D004355:Drug Stability; D006633:Histamine Antagonists / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D006633:Histamine Antagonists
keywords: UPLC; antihistamine; dietary supplement; validated