title: Analysis of phosphoproteome in rice pistil.
abstract: As the female reproductive part of a flower, the pistil consists of the ovary, style, and stigma, and is a critical organ for the process from pollen recognition to fertilization and seed formation. Previous studies on pollen-pistil interaction mainly focused on gene expression changes with comparative transcriptomics or proteomics method. However, studies on protein PTMs are still lacking. Here we report a phosphoproteomic study on mature pistil of rice. Using IMAC enrichment, hydrophilic interaction chromatography fraction and high-accuracy MS instrument (TripleTOF 5600), 2347 of high-confidence (Ascore ≥ 19, p ≤ 0.01), phosphorylation sites corresponding to 1588 phosphoproteins were identified. Among them, 1369 phosphorylation sites within 654 phosphoproteins were newly identified; 41 serine phosphorylation motifs, which belong to three groups: proline-directed, basophilic, and acidic motifs were identified after analysis by motif-X. Two hundred and one genes whose phosphopeptides were identified here showed tissue-specific expression in pistil based on information mining of previous microarray data. All MS data have been deposited in the ProteomeXchange with identifier PXD000923 (http://proteomecentral.proteomexchange.org/dataset/PXD000923). This study will help us to understand pistil development and pollination on the posttranslational level.
authors: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of Chinese Academy of Sciences, Wuhan, P. R. China.
authors: Kun
authors: K
authors: Wang
authors: Yong
authors: Y
authors: Zhao
authors: Ming
authors: M
authors: Li
authors: Feng
authors: F
authors: Gao
authors: Ming-Kun
authors: MK
authors: Yang
authors: Xin
authors: X
authors: Wang
authors: Shaoqing
authors: S
authors: Li
authors: Pingfang
authors: P
authors: Yang
mesh_terms: D000595:Amino Acid Sequence; D035264:Flowers / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry* / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis* / Q000378:metabolism; D010766:Phosphorylation; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D016415:Sequence Alignment
chemical_list: D010750:Phosphoproteins; D010940:Plant Proteins
keywords: IMAC; Oryza sativa L; Phosphoproteome; Pistil; Plant proteomics; Pollen-pistil interaction