title: Healing of critical-size segmental defects in rat femora using strong porous bioactive glass scaffolds.
abstract: The repair of structural bone defects such as segmental defects in the long bones of the limbs is a challenging clinical problem. In this study, the capacity of silicate (13-93) and borate (13-93B3) bioactive glass scaffolds (porosity=47-50%) to heal critical-size segmental defects in rat femurs was evaluated and compared with autografts. Defects were implanted with 13-93 and 13-93B3 scaffolds with a grid-like microstructure (compressive strength=86 MPa and 40 MPa, respectively), 13-93B3 scaffolds with an oriented microstructure (compressive strength=32 MPa) and autografts using intramedullary fixation. Twelve weeks post-implantation, the defects were harvested and evaluated using histomorphometric analysis. The percentage of new bone in the defects implanted with the three groups of glass scaffolds (25-28%) and the total von Kossa-positive area (32-38%) were not significantly different from the autografts (new bone=38%; von Kossa-positive area=40%) (p>0.05). New blood vessel area in the defects implanted with the glass scaffolds (4-8%) and the autografts (5%) showed no significant difference among the four groups. New cartilage formed in the 13-93 grid-like scaffolds (18%) was significantly higher than in 13-93B3 grid-like scaffolds (8%) and in the autografts (8%) (p=0.02). The results indicate that these strong porous bioactive glass scaffolds are promising synthetic implants for structural bone repair.
authors: Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri - Kansas City, Kansas City, MO 64108, USA.
authors: Lianxiang
authors: L
authors: Bi
authors: School of Dentistry, University of Missouri - Kansas City, Kansas City, MO 64108, USA.
authors: Brett
authors: B
authors: Zobell
authors: Department of Materials Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA.
authors: Xin
authors: X
authors: Liu
authors: Department of Materials Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA. Electronic address: rahaman@mst.edu.
authors: Mohamed N
authors: MN
authors: Rahaman
authors: Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri - Kansas City, Kansas City, MO 64108, USA. Electronic address: bonewaldl@umkc.edu.
authors: Lynda F
authors: LF
authors: Bonewald
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D002356:Cartilage / Q000378:metabolism; D005269:Femur / Q000187:drug effects* / Q000293:injuries / Q000502:physiology; D005898:Glass / Q000737:chemistry*; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: C553965:13-93 bioactive glass; D001672:Biocompatible Materials
keywords: Bioactive glass scaffold; Bone regeneration; Histomorphometric analysis; Rodent model; Segmental bone defects