title: Effects of tatariside G isolated from Fagopyrum tataricum roots on apoptosis in human cervical cancer HeLa cells.
abstract: Cervical cancer is the second most common female carcinoma. Current therapies are often unsatisfactory, especially for advanced stage patients. The aim of this study was to explore the effects of tatariside G (TG) on apoptosis in human cervical cancer HeLa cells and the possible mechanism of action involved. An MTT assay was employed to evaluate cell viability. Hoechst 33258 staining and flow cytometry (FCM) assays were used to detect cell apoptosis. The protein expression of phosphorylated JNK, P38, ERK and Akt and cleaved caspase-3 and caspase-9 was evaluated by western blot analysis. Additionally, the mRNA expression of caspase-3 and caspase-9 was measured by fluorescent quantitative reverse transcription-PCR (FQ-RT-PCR). TG notably inhibited cell viability, enhanced the percentage of apoptotic cells, facilitated the phosphorylation of p38 MAPK and JNK proteins and caspase-3 and caspase-9 cracking, downregulated the phosphorylation level of Akt, and increased the loss of MMP and the mRNA expression of caspase-3 and caspase-9. TG-induced apoptosis is associated with activation of the mitochondrial death pathway. TG may be an effective candidate for chemotherapy against cervical cancer.
authors: Department of Chinese Materia Medica, School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. mengyingfeihen@126.com.
authors: Yuan
authors: Y
authors: Li
authors: Central Laboratory, Shanghai Seventh People's Hospital, Shanghai 200137, China. xhwang911@163.com.
authors: Su-Juan
authors: SJ
authors: Wang
authors: Department of Nuclear Medicine, Shanghai Seventh People's Hospital, Shanghai 200137, China. awingxia@163.com.
authors: Wei
authors: W
authors: Xia
authors: School of Pharmacy and Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, Liverpool L3 3AF, England, UK. K.Rahman@ljmu.ac.uk.
authors: Khalid
authors: K
authors: Rahman
authors: Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. jinhu7963@163.com.
authors: Yan
authors: Y
authors: Zhang
authors: Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. ncxq19811031@163.com.
authors: Hao
authors: H
authors: Peng
authors: Central Laboratory, Shanghai Seventh People's Hospital, Shanghai 200137, China. jinhu7963@163.com.
authors: Hong
authors: H
authors: Zhang
authors: Department of Chinese Materia Medica, School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. qinluping@126.com.
authors: Lu-Ping
authors: LP
authors: Qin
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D019612:Fagopyrum / Q000737:chemistry*; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D010936:Plant Extracts; C000596340:tatariside G; D051057:Proto-Oncogene Proteins c-akt; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D053148:Caspase 3; D053453:Caspase 9