title: Diversity and spatiotemporal dynamics of bacterial communities: physicochemical and other drivers along an acid mine drainage.
abstract: Deciphering the biotic and abiotic factors that control microbial community structure over time and along an environmental gradient is a pivotal question in microbial ecology. Carnoulès mine (France), which is characterized by acid waters and very high concentrations of arsenic, iron, and sulfate, provides an excellent opportunity to study these factors along the pollution gradient of Reigous Creek. To this end, biodiversity and spatiotemporal distribution of bacterial communities were characterized using T-RFLP fingerprinting and high-throughput sequencing. Patterns of spatial and temporal variations in bacterial community composition linked to changes in the physicochemical conditions suggested that species-sorting processes were at work in the acid mine drainage. Arsenic, temperature, and sulfate appeared to be the most important factors that drove the composition of bacterial communities along this continuum. Time series investigation along the pollution gradient also highlighted habitat specialization for some major members of the community (Acidithiobacillus and Thiomonas), dispersal for Acidithiobacillus, and evidence of extinction/re-thriving processes for Gallionella. Finally, pyrosequencing revealed a broader phylogenetic range of taxa than previous clone library-based diversity. Overall, our findings suggest that in addition to environmental filtering processes, additional forces (dispersal, birth/death events) could operate in AMD community.
authors: Laboratoire HydroSciences Montpellier, HSM, UMR 5569 (IRD, CNRS, Universités Montpellier 1 et 2), Université Montpellier 2, Montpellier, France.
authors: Aurélie
authors: A
authors: Volant
authors: Odile
authors: O
authors: Bruneel
authors: Angélique
authors: A
authors: Desoeuvre
authors: Marina
authors: M
authors: Héry
authors: Corinne
authors: C
authors: Casiot
authors: Noëlle
authors: N
authors: Bru
authors: Sophie
authors: S
authors: Delpoux
authors: Anne
authors: A
authors: Fahy
authors: Fabien
authors: F
authors: Javerliat
authors: Olivier
authors: O
authors: Bouchez
authors: Robert
authors: R
authors: Duran
authors: Philippe N
authors: PN
authors: Bertin
authors: Françoise
authors: F
authors: Elbaz-Poulichet
authors: Béatrice
authors: B
authors: Lauga
mesh_terms: D001151:Arsenic / Q000032:analysis; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D007501:Iron / Q000032:analysis; D008906:Mining; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D013431:Sulfates / Q000032:analysis; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis; D014876:Water Pollution
chemical_list: D013431:Sulfates; D014874:Water Pollutants, Chemical; D007501:Iron; D001151:Arsenic
keywords: acid mine drainage; arsenic; bacterial diversity; spatial and temporal dynamics