title: Oxovanadium(IV) complexes of curcumin for cellular imaging and mitochondria targeted photocytotoxicity.
abstract: Oxovanadium(IV) complexes [VO(R-tpy)(cur)](ClO4) (1, 2) of curcumin (Hcur) and terpyridine ligands (R-tpy) where R is phenyl (phtpy in 1) or p-triphenylphosphonium methylphenyl bromide (C6H4CH2PPh3Br) (TPP-phtpy in 2) were prepared and characterized and their DNA photocleavage activity, photocytotoxicity and cellular localization in cancer cells (HeLa and MCF-7) were studied. Acetylacetonate (acac) complexes [VO(R-tpy)(acac)](ClO4) of phtpy (3) and TPP-phtpy (4) were prepared and used as the control species. These complexes showed efficient cleavage of pUC19 DNA in visible light of 454 nm and near-IR light of 705 nm. Complexes 1 and 2 showed significant photocytotoxicity in visible light of 400-700 nm. FACS analysis showed sub-G1/G0 phase cell-cycle arrest in cancer cells when treated with 1 and 2 in visible light in comparison with the dark controls. Fluorescence microscopic studies revealed specific localization of the p-triphenylphosphonium complex 2 in the mitochondria of MCF-7 cancer cells whereas no such specificity was observed for complex 1.
authors: Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore 560012, India. arc@ipc.iisc.ernet.in.
authors: Bhabatosh
authors: B
authors: Banik
authors: Kumar
authors: K
authors: Somyajit
authors: Ganesh
authors: G
authors: Nagaraju
authors: Akhil R
authors: AR
authors: Chakravarty
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage / Q000737:chemistry*; D003603:Cytotoxins / Q000008:administration & dosage / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D018613:Microscopy, Confocal / Q000379:methods; D008928:Mitochondria / Q000737:chemistry* / Q000187:drug effects; D010775:Photic Stimulation / Q000379:methods; D014638:Vanadates / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D003603:Cytotoxins; C075743:oxovanadium IV; D014638:Vanadates; D003474:Curcumin