title: Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.
abstract: Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since standard kits to produce cDNA of small RNAs are biased for certain small RNAs, we used High Definition adapters that reduce ligation bias. We identified and experimentally validated five new microRNAs and a few other small non-coding RNAs that were not microRNAs. The biological implication of microRNA expression levels and targets of microRNAs are discussed.
authors: School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
authors: Ping
authors: P
authors: Xu
authors: School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
authors: Irina
authors: I
authors: Mohorianu
authors: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Centre for Bamboo, Rattan, Beijing, China.
authors: Li
authors: L
authors: Yang
authors: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Centre for Bamboo, Rattan, Beijing, China.
authors: Hansheng
authors: H
authors: Zhao
authors: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Centre for Bamboo, Rattan, Beijing, China.
authors: Zhimin
authors: Z
authors: Gao
authors: School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
authors: Tamas
authors: T
authors: Dalmay
mesh_terms: D031723:Bambusa / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics; D018515:Plant Leaves / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant