title: Leukemic marker detection using a spectro-polarimetric surface plasmon resonance platform.
abstract: In this paper, we present a proof of concept screening for monoclonal immunoglobulin as a leukemia tumor marker using a surface plasmon resonance (SPR) bio-sensing platform. This screening method is based on measurements of immunoglobulin levels in human serum and the determination of the relative concentrations of kappa and lambda light chains. The kappa/lambda ratio is used to determine the presence of monoclonal immunoglobulin. Tests have been performed using standard solutions of immunoglobulins and serum samples from patients with known leukemic diagnoses. This platform has a resolution of 5×10(-7) refractive index unit (RIU) per channel, which is up to 10 times better than other SPR imaging systems for multi-sensing applications. The results obtained with this technique are in agreement with those acquired using conventional methods for immunoglobulin detection, indicating that our polarimetric SPR platform should be suitable for a cheap and efficient tool for early leukemia biomarker screening and monitoring applications.
authors: Department of Engineering Physics, École Polytechnique de Montreal, Montreal, QC, Canada.
authors: Mathieu
authors: M
authors: Maisonneuve
authors: Department of Chemistry, University of Victoria, Victoria, BC, Canada.
authors: Chiara
authors: C
authors: Valsecchi
authors: Department of Pathology and Lab Medicine, Mount Sinai Hospital, University of Toronto, Toronto, ON, Canada.
authors: Chen
authors: C
authors: Wang
authors: Department of Chemistry, University of Victoria, Victoria, BC, Canada.
authors: Alexandre G
authors: AG
authors: Brolo
authors: Department of Engineering Physics, École Polytechnique de Montreal, Montreal, QC, Canada. Electronic address: michel.meunier@polymtl.ca.
authors: Michel
authors: M
authors: Meunier
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000302:isolation & purification*; D015374:Biosensing Techniques; D006801:Humans; D007136:Immunoglobulins / Q000097:blood* / Q000302:isolation & purification; D007938:Leukemia / Q000097:blood* / Q000235:genetics / Q000276:immunology; D020349:Surface Plasmon Resonance
chemical_list: D014408:Biomarkers, Tumor; D007136:Immunoglobulins
keywords: Antibodies detection; Leukemia screening; SPR sensor; Spectro-polarimetric imaging