title: Facile enzymatic synthesis of base J-containing oligodeoxyribonucleotides and an analysis of the impact of base J on DNA replication in cells.
abstract: We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU) in both single- and double-stranded ODNs, though the latter reaction occurred only when 5hmU was mispaired with a guanine. In addition, base J blocked moderately DNA replication, but it did not induce mutations during replication in human cells.
authors: Department of Chemistry, University of California Riverside, Riverside, California, United States of America.
authors: Debin
authors: D
authors: Ji
authors: Department of Chemistry, University of California Riverside, Riverside, California, United States of America.
authors: Yinsheng
authors: Y
authors: Wang
mesh_terms: D017122:Bacteriophage T4 / Q000201:enzymology; D055162:Biocatalysis; D004261:DNA Replication / Q000187:drug effects*; D005960:Glucosides / Q000737:chemistry* / Q000494:pharmacology; D005964:Glucosyltransferases / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D005960:Glucosides; D009838:Oligodeoxyribonucleotides; D014764:Viral Proteins; C084880:5-((glucopyranosyloxy)methyl)uracil; D014498:Uracil; D005964:Glucosyltransferases