title: Up-regulation of 91H promotes tumor metastasis and predicts poor prognosis for patients with colorectal cancer.
abstract: BACKGROUND
  Long noncoding RNAs (lncRNAs) play widespread roles in gene regulation and cellular processes. However, the functional roles of lncRNAs in colorectal cancer (CRC) are not yet well elucidated. The aim of the present study was to measure the levels of lncRNA 91H expression in CRC and evaluate its clinical significance and biological roles in the development and progression of CRC.
  
  
  METHODS
  91H expression and copy number variation (CNV) were measured in 72 CRC tumor tissues and adjacent normal tissues by real-time PCR. The biological roles of 91H were evaluated by MTT, scratch wound assay, migration and invasion assays, and flow cytometry.
  
  
  RESULTS
  91H was significantly overexpressed in cancerous tissue and CRC cell lines compared with adjacent normal tissue and a normal human intestinal epithelial cell line. Moreover, 91H overexpression was closely associated with distant metastasis and poor prognosis in patients with CRC, except for CNV of 91H. Multivariate analysis indicated that 91H expression was an independent prognostic indicator, as well as distant metastasis. Our in vitro data indicated that knockdown of 91H inhibited the proliferation, migration, and invasiveness of CRC cells.
  
  
  CONCLUSIONS
  91H played an important role in the molecular etiology of CRC and might be regarded as a novel prognosis indicator in patients with CRC.
authors: Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors: Qiwen
authors: Q
authors: Deng
authors: Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors: Bangshun
authors: B
authors: He
authors: Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors: Tianyi
authors: T
authors: Gao
authors: Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors: Yuqin
authors: Y
authors: Pan
authors: Department of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, China.
authors: Huiling
authors: H
authors: Sun
authors: Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors: Yeqiong
authors: Y
authors: Xu
authors: Department of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, China.
authors: Rui
authors: R
authors: Li
authors: Medical college, Southeast University, Nanjing, Jiangsu, China.
authors: Houqun
authors: H
authors: Ying
authors: Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors: Feng
authors: F
authors: Wang
authors: Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors: Xian
authors: X
authors: Liu
authors: Department of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, China.
authors: Jie
authors: J
authors: Chen
authors: Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors: Shukui
authors: S
authors: Wang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics; D002460:Cell Line; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality*; D056915:DNA Copy Number Variations; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D016019:Survival Analysis; D015854:Up-Regulation / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding