title: Immunohistochemical expression of heat shock proteins, p63 and androgen receptor in benign prostatic hyperplasia and prostatic carcinoma in the dog.
abstract: This study compared heat shock proteins Hsp60, Hsp72 and Hsp73, along with p63 and androgen receptor (AR) immunoexpression between 16 cases of benign prostatic hyperplasia (BPH) and 11 prostatic carcinomas (PCa) in dogs. The proportion of Hsp60-positive cells was higher in PCa compared with BPH (P = 0.033), whereas the frequency and intensity of Hsp73 immunostaining did not differ significantly between the two groups. Hsp72-immunostained nuclei formed a discontinuous layer along the basement membrane in BPH, whereas cells in this layer in PCa were negative or weakly positive. Hsp72 nuclear score showed significant positive associations with both p63 (P = 0.016) and AR (P = 0.009) scores. Double immunofluorescence revealed Hsp72-p63 and Hsp72-AR co-expressions in basal cell nuclei. Aberrant cytoplasmic p63 immunolabelling was observed in 3 of 11 PCa cases. These results suggest a role of the combined expression of Hsp72, p63 and AR in basal epithelial cells in canine BPH and PCa.
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: M
authors: M
authors: Romanucci
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: L
authors: L
authors: Frattone
authors: Faculty of Political Sciences, University of Teramo, Teramo, Italy.
authors: A
authors: A
authors: Ciccarelli
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: L
authors: L
authors: Bongiovanni
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: D
authors: D
authors: Malatesta
authors: Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia (Bologna), Italy.
authors: C
authors: C
authors: Benazzi
authors: Department of Biopathological Sciences and Hygiene of Animal and Food Production, Faculty of Veterinary Medicine, University of Perugia, Perugia, Italy.
authors: C
authors: C
authors: Brachelente
authors: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors: L
authors: L
authors: Della Salda
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000378:metabolism / Q000662:veterinary; D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D011470:Prostatic Hyperplasia / Q000378:metabolism / Q000662:veterinary*; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000662:veterinary; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018834:Chaperonin 60; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D011944:Receptors, Androgen; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins
keywords: androgen receptor; benign prostatic hyperplasia; dog; heat shock protein; p63; prostate cancer