title: Protective effect of quercetin in ecto-enzymes, cholinesterases, and myeloperoxidase activities in the lymphocytes of rats exposed to cadmium.
abstract: The ex vivo and in vitro effects of quercetin on NTPDase, adenosine deaminase (ADA), and acetycholinesterase (AChE) activities in lymphocytes, as well as the effects of quercetin on butyrylcholinesterase (BChE) activity in serum and myeloperoxidase (MPO) activity in plasma were determined in rats. For the ex vivo experiment, animals were orally exposed to Cadmium (Cd) for 45 days. Animals were divided into eight groups: saline/ethanol, saline/Querc 5 mg/kg, saline/Querc 25 mg/kg, saline/Querc 50 mg/kg, Cd/ethanol, Cd/Querc 5 mg/kg, Cd/Querc 25 mg/kg, and Cd/Querc 50 mg/kg. The ex vivo data showed an increase in the ATP and ADP hydrolysis and ADA activity in Cd-exposed rats when compared to the control group. The treatment with quercetin 25 and 50 mg/kg prevented this increase in the ATP and ADP hydrolysis, while the treatment with quercetin 5, 25, and 50 mg/kg prevented the increase in the ADA activity. AChE, BChE, and MPO activities ex vivo presented an increase in the Cd-exposed group when compared to the control group, and the treatment with quercetin 5, 25, and 50 mg/kg prevented this increase caused by Cd exposure. The in vitro experiment showed that quercetin 5, 10, 25, or 50 µM decreased the ADA activity proportionally to the increase of the concentrations of quercetin when compared to the control group. Thus, we can suggest that the quercetin is able to modulate NTPDase, ADA, AChE, and MPO activities and contribute to maintain the levels of ATP, adenosine, and acetylcholine normal, respectively, exhibiting potent pro-inflammatory and anti-inflammatory actions.
authors: Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, Santa Maria, RS, 97105-900, Brazil, faha.biomed@hotmail.com.
authors: Fátima Husein
authors: FH
authors: Abdalla
authors: Andréia Machado
authors: AM
authors: Cardoso
authors: Roberta
authors: R
authors: Schmatz
authors: Jamile Fabbrin
authors: JF
authors: Gonçalves
authors: Jucimara
authors: J
authors: Baldissarelli
authors: Caroline Curry
authors: CC
authors: Martins
authors: Daniela
authors: D
authors: Zanini
authors: Lizielle Souza
authors: LS
authors: de Oliveira
authors: Pauline
authors: P
authors: da Costa
authors: Victor Camera
authors: VC
authors: Pimentel
authors: Luciane Belmonte
authors: LB
authors: Pereira
authors: Cibele Lima
authors: CL
authors: Lhamas
authors: Maria Rosa Chitolina
authors: MR
authors: Schetinger
authors: Vera Maria
authors: VM
authors: Morsch
authors: Cinthia Melazzo Andrade
authors: CM
authors: Mazzanti
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood; D002104:Cadmium / Q000633:toxicity*; D002802:Cholinesterases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006868:Hydrolysis; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000378:metabolism*; D020011:Protective Agents / Q000494:pharmacology; D011755:Pyrophosphatases / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D017208:Rats, Wistar; D018675:Toxicity Tests / Q000379:methods
chemical_list: D020011:Protective Agents; D002104:Cadmium; D011794:Quercetin; D009195:Peroxidase; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases; D000243:Adenosine Deaminase; D011755:Pyrophosphatases