title: [Effect of glycolytic inhibitor 3-BrPA on the proliferation and apoptosis of mouse splenic lymphocytes in mixed lymphocytes culture].
abstract: OBJECTIVE
  To study the effect of glycolytic inhibitor 3-Bromopyruvate (3-BrPA) on the proliferation and apoptosis of mouse spleen lymphocytes and explore its mechanism.
  
  
  METHODS
  An one-way mixed lymphocyte culture (MLC) system was established, including BALB/c mouse spleen cells (H-2d) as stimulator and C57BL/6 mouse spleen cells (H-2b) as responder. With treatment of 3-BrPA at different concentrations (0-200 μmol/L), lymphocyte proliferation capacity was detected by the CCK-8 method, the expression of CD3, CD4, and CD8 by flow cytometry, and the concentrations of cytokine interleukin (IL)-4 and interferon (IFN)-γ in the supernatant by ELISA.
  
  
  RESULTS
  At a middle or high dose (over 20 μmol/L), 3-BrPA displayed a dose-dependent inhibitory effect on lymphocyte proliferation in the MLC system. The 50% inhibitory concentration (IC50) were 48.6, 41.2, and 41.9 μmol/L after 24, 36, and 48 h culture, respectively. With treatment of 50 μmol/L 3-BrPA, the IFN-γ level [(164.25 ± 20.14) ng/L] was significantly lower, compared with control [(277.61 ± 18.46) ng/L]. The IL-4 level [(31.06 ± 6.06) ng/L] was significantly higher, compared with control [(28.64 ± 3.97) ng/L]. Consequently, the IFN-γ/IL-4 ratio decreased significantly.
  
  
  CONCLUSIONS
  These results indicate that 3-BrPA had a significant inhibitory effect on the proliferation of mouse spleen lymphocytes cultured in MLC system, accompanied with the Th2-biased secretion of cytokines.
authors: Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University Zhongshan 528403, China.
authors: Ruiqing
authors: R
authors: Zhou
authors: Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University Zhongshan 528403, China.
authors: Jing
authors: J
authors: Wang
authors: Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University Zhongshan 528403, China.
authors: Dafa
authors: D
authors: Qiu
authors: Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University Zhongshan 528403, China.
authors: Xiaomin
authors: X
authors: Niu
authors: Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University Zhongshan 528403, China.
authors: Ziwen
authors: Z
authors: Guo
authors: Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University Zhongshan 528403, China.
authors: Huiqing
authors: H
authors: He
authors: Department of Hematology, Zhongshan Hospital of Sun Yat-Sen University Zhongshan 528403, China.
authors: Xiaojun
authors: X
authors: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011773:Pyruvates / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology* / Q000378:metabolism
chemical_list: D011773:Pyruvates; D015847:Interleukin-4; C017092:bromopyruvate; D007371:Interferon-gamma