title: Effect of glucose content on thermally cross-linked fibrous gelatin scaffolds for tissue engineering.
abstract: Thermally cross-linked glucose-containing electrospun gelatin meshes were studied as possible cell substrate materials. FTIR analysis was used to study the effect of glucose on cross-linking reactions. It was found that the presence of glucose increases the extent of cross-linking of fibrous gelatin scaffolds, which in return determines scaffold properties and their usability in tissue engineering applications. Easy to handle fabric-like scaffolds were obtained from blends containing up to 15% glucose. Maximum extent of cross-linking was reached at nearly 20% glucose content. Cross-linking effectively resulted in decreased solubility and increased resistance to enzymatic degradation. Preliminary short-term cell culture experiments indicate that such thermally cross-linked gelatin-glucose scaffolds are suitable for tissue engineering applications.
authors: Institute of Physics, University of Tartu, Estonia. Electronic address: Kaido.Siimon@ut.ee.
authors: Kaido
authors: K
authors: Siimon
authors: Institute of Biomedicine and Translational Medicine, University of Tartu, Estonia.
authors: Paula
authors: P
authors: Reemann
authors: Faculty of Medicine, University of Tartu, Estonia.
authors: Annika
authors: A
authors: Põder
authors: Institute of Molecular and Cell Biology, University of Tartu, Estonia.
authors: Martin
authors: M
authors: Pook
authors: Institute of Physics, University of Tartu, Estonia; Estonian Nanotechnology Competence Centre, Estonia.
authors: Triin
authors: T
authors: Kangur
authors: Clinic of Dermatology, Tartu University Hospital, Estonia; Department of Dermatology, University of Tartu, Estonia.
authors: Külli
authors: K
authors: Kingo
authors: Institute of Molecular and Cell Biology, University of Tartu, Estonia.
authors: Viljar
authors: V
authors: Jaks
authors: Institute of Chemistry, University of Tartu, Estonia.
authors: Uno
authors: U
authors: Mäeorg
authors: Institute of Physics, University of Tartu, Estonia; Estonian Nanotechnology Competence Centre, Estonia.
authors: Martin
authors: M
authors: Järvekülg
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D055664:Electrochemical Techniques; D005347:Fibroblasts / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D036103:Nanotechnology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D005780:Gelatin; D005947:Glucose
keywords: Cross-linking; Electrospinning; Fibroblast; Gelatin; Glucose