title: Metabolic characterization of hematopoietic stem cells.
abstract: An important feature of stem cells is their maintenance in their respective hypoxic niche. Survival in this low-oxygen microenvironment requires significant metabolic adaptation. We demonstrated that mouse HSCs utilize glycolysis instead of mitochondrial oxidative phosphorylation to meet their energy demands. We have adapted various tools for characterization of the metabolic properties of hematopoietic stem cells (HSCs). These techniques include flow cytometric profiling of HSCs based on mitochondrial potential and NADH fluorescence as well as measurement of ATP content, oxygen consumption rate, and glycolytic flux in purified HSCs.
authors: Department of Education, and Texas Institute of Biotechnology, Education and Research, North American University, 3203 N. Sam Houston Pkwy W, Houston, TX, 77038, USA, fk@na.edu.
authors: Fatih
authors: F
authors: Kocabas
authors: Junke
authors: J
authors: Zheng
authors: Chengcheng
authors: C
authors: Zhang
authors: Hesham A
authors: HA
authors: Sadek
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques; D005434:Flow Cytometry; D008401:Gas Chromatography-Mass Spectrometry; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D010101:Oxygen Consumption; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate