title: Isolation, screening and identification of Swine gut microbiota with ochratoxin a biodegradation ability.
abstract: The potential for ochratoxin A (OTA) degradation by swine intestinal microbiota was assessed in the current study. Intestinal content that was collected aseptically from swine was spiked with 100 ppb OTA and incubated for 6 and 12 h at 39°C. An OTA assay was conducted using the incubated samples, and it was found that 20% of the OTA toxin was detoxified, indicating the presence of microbes capable of OTA degradation. Twenty-eight bacterial species were isolated anaerobically in M 98-5 media and 45 bacterial species were isolated using nutrient broth aerobically. Screening results showed that one anaerobic bacterial isolate, named MM11, detoxified more than 75% of OTA in liquid media. Furthermore, 1.0 ppm OTA was degraded completely after 24 h incubation on a solid 'corn' substrate. The bacterium was identified by 16S rDNA sequencing as having 97% sequence similarity with Eubacterium biforme. The isolation of an OTA-degrading bacterium from the swine natural flora is of great importance for OTA biodegradation and may be a valuable potential source for OTA-degradation enzymes in industrial applications.
authors: Genebiotech co., Ltd., Gongju, Republic of Korea .
authors: Santi Devi
authors: SD
authors: Upadhaya
authors: Genebiotech co., Ltd., Gongju, Republic of Korea .
authors: Jae Yong
authors: JY
authors: Song
authors: Genebiotech co., Ltd., Gongju, Republic of Korea .
authors: Min Ah
authors: MA
authors: Park
authors: Genebiotech co., Ltd., Gongju, Republic of Korea .
authors: Ja Kyeom
authors: JK
authors: Seo
authors: Genebiotech co., Ltd., Gongju, Republic of Korea .
authors: Liu
authors: L
authors: Yang
authors: Genebiotech co., Ltd., Gongju, Republic of Korea .
authors: Chan Ho
authors: CH
authors: Lee
authors: Genebiotech co., Ltd., Gongju, Republic of Korea .
authors: Kyung J
authors: KJ
authors: Cho
authors: Genebiotech co., Ltd., Gongju, Republic of Korea .
authors: Jong K
authors: JK
authors: Ha
keywords: Degradation; Enzyme-linked Immunosorbent Assay; Isolation; Ochratoxin A; Swine Intestinal Microbiota