title: Highly sensitive multianalyte immunochromatographic test strip for rapid chemiluminescent detection of ractopamine and salbutamol.
abstract: A novel immunochromatographic assay (ICA) was proposed for rapid and multiple assay of β2-agonists, by utilizing ractopamine (RAC) and salbutamol (SAL) as the models. Owing to the introduction of chemiluminescent (CL) approach, the proposed protocol shows much higher sensitivity. In this work, the described ICA was based on a competitive format, and horseradish peroxidase-tagged antibodies were used as highly sensitive CL probes. Quantitative analysis of β2-agonists was achieved by recording the CL signals of the probes captured on the two test zones of the nitrocellulose membrane. Under the optimum conditions, RAC and SAL could be detected within the linear ranges of 0.50-40 and 0.10-50 ng mL(-1), with the detection limits of 0.20 and 0.040 ng mL(-1) (S/N=3), respectively. The whole process for multianalyte immunoassay of RAC and SAL can be completed within 20 min. Furthermore, the test strip was validated with spiked swine urine samples and the results showed that this method was reliable in measuring β2-agonists in swine urine. This CL-based multianalyte test strip shows a series of advantages such as high sensitivity, ideal selectivity, simple manipulation, high assay efficiency and low cost. Thus, it opens up new pathway for rapid screening and field analysis, and shows a promising prospect in food safety.
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors: Hongfei
authors: H
authors: Gao
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors: Jing
authors: J
authors: Han
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors: Shijia
authors: S
authors: Yang
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors: Zhenxing
authors: Z
authors: Wang
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
authors: Lin
authors: L
authors: Wang
authors: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. Electronic address: fuzf@swu.edu.cn.
authors: Zhifeng
authors: Z
authors: Fu
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000032:analysis* / Q000652:urine; D000420:Albuterol / Q000032:analysis* / Q000652:urine; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D010627:Phenethylamines / Q000032:analysis* / Q000652:urine; D013552:Swine
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D010627:Phenethylamines; C060897:ractopamine; D000420:Albuterol
keywords: Chemiluminescence; Immunochromatographic test strip; Lean meat agent; Multianalyte immunoassay; β(2)-agonist