title: A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo.
abstract: In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
authors: Kathryn S
authors: KS
authors: Potts
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
authors: Tobias J
authors: TJ
authors: Sargeant
authors: National Information Communications Technology Australia Victoria Research Laboratory, Department of Electrical and Electronic Engineering, School of Engineering.
authors: John F
authors: JF
authors: Markham
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Computing and Information Systems, and Department of Mathematics and Statistics, The University of Melbourne, Australia; and.
authors: Wei
authors: W
authors: Shi
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
authors: Christine
authors: C
authors: Biben
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
authors: Emma C
authors: EC
authors: Josefsson
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
authors: Lachlan W
authors: LW
authors: Whitehead
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
authors: Kelly L
authors: KL
authors: Rogers
authors: Medical Research Council Centre for Regenerative Medicine, University of Edinburgh, United Kingdom.
authors: Anna
authors: A
authors: Liakhovitskaia
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Computing and Information Systems, and Department of Mathematics and Statistics, The University of Melbourne, Australia; and.
authors: Gordon K
authors: GK
authors: Smyth
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
authors: Benjamin T
authors: BT
authors: Kile
authors: Medical Research Council Centre for Regenerative Medicine, University of Edinburgh, United Kingdom.
authors: Alexander
authors: A
authors: Medvinsky
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
authors: Warren S
authors: WS
authors: Alexander
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
authors: Douglas J
authors: DJ
authors: Hilton
authors: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
authors: Samir
authors: S
authors: Taoudi
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000235:genetics*; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics / Q000378:metabolism; D004171:Diploidy; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D055016:Megakaryocyte Progenitor Cells / Q000166:cytology / Q000378:metabolism*; D008533:Megakaryocytes / Q000166:cytology / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D020411:Oligonucleotide Array Sequence Analysis; D011123:Polyploidy; D013997:Time Factors; D059467:Transcriptome; D015017:Yolk Sac / Q000166:cytology / Q000196:embryology / Q000378:metabolism
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; C493726:Runx1 protein, mouse