title: Rgn gene is required for gut cell homeostasis after ingestion of sodium dodecyl sulfate in Drosophila.
abstract: Resistance and resilience constitute the two complementary aspects of epithelial host defenses in Drosophila. Epithelial cell homeostasis is necessary for the recovery of damages caused by stress or infections. However, the genes responsible for gut epithelial homeostasis remain poorly understood. Here, we show that rgn(G4035) mutant flies have higher mortality than wild-type flies after ingestion of sodium dodecyl sulfate (SDS). Excessive melanization and increased necrotic cells in the gut contribute to the reduced survival of rgn(G4035) mutant flies following SDS ingestion. rgn mutant flies have a defect in the replenishment of intestinal stem cells (ISCs) following gut damage. The antimicrobial peptide (AMP) expression is affected in rgn(G4035) mutant fly guts. Together, our study provides evidence that rgn gene is essential for gut cell homeostasis following damage in Drosophila.
authors: College of Life Science, Northeast Forestry University, No. 26 Hexing Road, Xiangfang District, Harbin 150040, PR China.
authors: Jichuan
authors: J
authors: Pan
authors: College of Life Science, Northeast Forestry University, No. 26 Hexing Road, Xiangfang District, Harbin 150040, PR China. Electronic address: lhjin2000@hotmail.com.
authors: Li Hua
authors: LH
authors: Jin
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D008297:Male; D008543:Melanins / Q000378:metabolism; D009154:Mutation; D009336:Necrosis; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013234:Stem Cells / Q000378:metabolism
chemical_list: D023181:Antimicrobial Cationic Peptides; C054756:DptA protein, Drosophila; D029721:Drosophila Proteins; D008543:Melanins; C529789:Rgn protein, Drosophila; C091227:DRS protein, Drosophila; D012967:Sodium Dodecyl Sulfate; C031161:cecropin A
keywords: 7-Aminoactinomycin D; Antimicrobial peptide; Melanization; Mutant; Survival