title: Comparative mapping between Coho Salmon (Oncorhynchus kisutch) and three other salmonids suggests a role for chromosomal rearrangements in the retention of duplicated regions following a whole genome duplication event.
abstract: Whole genome duplication has been implicated in evolutionary innovation and rapid diversification. In salmonid fishes, however, whole genome duplication significantly pre-dates major transitions across the family, and re-diploidization has been a gradual process between genomes that have remained essentially collinear. Nevertheless, pairs of duplicated chromosome arms have diverged at different rates from each other, suggesting that the retention of duplicated regions through occasional pairing between homeologous chromosomes may have played an evolutionary role across species pairs. Extensive chromosomal arm rearrangements have been a key mechanism involved in re-dipliodization of the salmonid genome; therefore, we investigated their influence on degree of differentiation between homeologs across salmon species. We derived a linkage map for coho salmon and performed comparative mapping across syntenic arms within the genus Oncorhynchus, and with the genus Salmo, to determine the phylogenetic relationship between chromosome arrangements and the retention of undifferentiated duplicated regions. A 6596.7 cM female coho salmon map, comprising 30 linkage groups with 7415 and 1266 nonduplicated and duplicated loci, respectively, revealed uneven distribution of duplicated loci along and between chromosome arms. These duplicated regions were conserved across syntenic arms across Oncorhynchus species and were identified in metacentric chromosomes likely formed ancestrally to the divergence of Oncorhynchus from Salmo. These findings support previous studies in which observed pairings involved at least one metacentric chromosome. Re-diploidization in salmon may have been prevented or retarded by the formation of metacentric chromosomes after the whole genome duplication event and may explain lineage-specific innovations in salmon species if functional genes are found in these regions.
authors: School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98105 mkodama@uw.edu knaish@uw.edu.
authors: Miyako
authors: M
authors: Kodama
authors: School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98105.
authors: Marine S O
authors: MS
authors: Brieuc
authors: Fisheries and Oceans Canada, West Vancouver, British Columbia, V7K 1N6, Canada.
authors: Robert H
authors: RH
authors: Devlin
authors: National Marine Fisheries Service, Northwest Fisheries Science Center, Seattle, Washington 98112.
authors: Jeffrey J
authors: JJ
authors: Hard
authors: School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98105 mkodama@uw.edu knaish@uw.edu.
authors: Kerry A
authors: http://orcid.org/0000-0002-3275-8778
authors: KA
authors: Naish
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D020440:Gene Duplication; D008040:Genetic Linkage; D016678:Genome; D008297:Male; D012474:Salmon / Q000235:genetics*; D017422:Sequence Analysis, DNA
keywords: RAD sequencing; chromsome rearrangements; comparative genome mapping; salmon; whole genome duplication