title: Sensitivity and specificity of the empirical lymphocyte genome sensitivity (LGS) assay: implications for improving cancer diagnostics.
abstract: Lymphocyte responses from 208 individuals: 20 with melanoma, 34 with colon cancer, and 4 with lung cancer (58), 18 with suspected melanoma, 28 with polyposis, and 10 with COPD (56), and 94 healthy volunteers were examined. The natural logarithm of the Olive tail moment (OTM) was plotted for exposure to UVA through 5 different agar depths (100 cell measurements/depth) and analyzed using a repeated measures regression model. Responses of patients with cancer plateaued after treatment with different UVA intensities, but returned toward control values for healthy volunteers. For precancerous conditions and suspected cancers, intermediate responses occurred. ROC analysis of mean log OTMs, for cancers plus precancerous/suspect conditions vs. controls, cancer vs. precancerous/suspect conditions plus controls, and cancer vs. controls, gave areas under the curve of 0.87, 0.89, and 0.93, respectively (P<0.001). Optimization allowed test sensitivity or specificity to approach 100% with acceptable complementary measures. This modified comet assay could represent a stand-alone test or an adjunct to other investigative procedures for detecting cancer.
authors: School of Life Sciences and d.anderson1@bradford.ac.uk.
authors: Diana
authors: D
authors: Anderson
authors: School of Life Sciences and.
authors: Mojgan
authors: M
authors: Najafzadeh
authors: School of Life Sciences and.
authors: Rajendran
authors: R
authors: Gopalan
authors: School of Life Sciences and.
authors: Nader
authors: N
authors: Ghaderi
authors: School of Health Studies, University of Bradford, Bradford, UK;
authors: Andrew J
authors: AJ
authors: Scally
authors: Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, UK; and.
authors: Stephen T
authors: ST
authors: Britland
authors: Bradford Royal Infirmary, Bradford, UK.
authors: Badie K
authors: BK
authors: Jacobs
authors: Bradford Royal Infirmary, Bradford, UK.
authors: P Dominic
authors: PD
authors: Reynolds
authors: Bradford Royal Infirmary, Bradford, UK.
authors: Justin
authors: J
authors: Davies
authors: Bradford Royal Infirmary, Bradford, UK.
authors: Andrew L
authors: AL
authors: Wright
authors: Bradford Royal Infirmary, Bradford, UK.
authors: Shariff
authors: S
authors: Al-Ghazal
authors: Bradford Royal Infirmary, Bradford, UK.
authors: David
authors: D
authors: Sharpe
authors: School of Life Sciences and.
authors: Morgan C
authors: MC
authors: Denyer
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003627:Data Interpretation, Statistical; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D015894:Genome, Human; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000175:diagnosis*; D011836:Radiation Tolerance; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
keywords: blood test; susceptibility