title: Addition of 1,3-β-D-glucan to chitosan-based composites enhances osteoblast adhesion, growth, and proliferation.
abstract: The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells. Cytotoxicity of materials was evaluated indirectly using fluid extracts and by direct-contact method using live/dead double fluorescent staining. Cell adhesion was determined quantitatively by LDH total test and cell proliferation was assessed by confocal microscope observation. Obtained data clearly prove that addition of 1,3-β-D-glucan to the bi-component chitosan/bioceramic materials significantly enhances adhesion, growth, and proliferation of osteoblast cells. The results demonstrated that all investigated biomaterials were non-toxic and allowed for cell attachment. However, significantly better osteoblast growth was observed on scaffolds containing 1,3-β-D-glucan. Thus, it may be inferred that scaffolds modified with glucan are more promising materials for bone tissue engineering application than bi-component chitosan/bioceramic composites.
authors: Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland. Electronic address: agata.przekora@umlub.pl.
authors: Agata
authors: A
authors: Przekora
authors: Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland.
authors: Grazyna
authors: G
authors: Ginalska
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D047071:beta-Glucans / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D047071:beta-Glucans; D048271:Chitosan; C033363:beta-1,3-glucan
keywords: Biocompatibility; Curdlan.; Scaffolds; Tissue engineering