title: Overexpression of Mps1 in colon cancer cells attenuates the spindle assembly checkpoint and increases aneuploidy.
abstract: The spindle assembly checkpoint kinase Mps1 is highly expressed in several types of cancers, but its cellular involvement in tumorigenesis is less defined. Herein, we confirm that Mps1 is overexpressed in colon cancer tissues. Further, we find that forced expression of Mps1 in the colon cancer cell line SW480 enables cells to become resistant to both Mps1 inhibition-induced checkpoint depletion and cell death. Overexpression of Mps1 also increases genome instability in tumor cells owing to a weakened spindle assembly checkpoint. Collectively, our findings suggest that high levels of Mps1 contribute to tumorigenesis by attenuating the spindle assembly checkpoint.
authors: Department of Life Science, Anhui University, Hefei, China; Beijing Institute of Biotechnology, Beijing, China.
authors: Youguo
authors: Y
authors: Ling
authors: Center for Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors: Xiaojuan
authors: X
authors: Zhang
authors: Beijing Institute of Biotechnology, Beijing, China.
authors: Yuanyuan
authors: Y
authors: Bai
authors: Beijing Institute of Biotechnology, Beijing, China.
authors: Ping
authors: P
authors: Li
authors: Beijing Institute of Biotechnology, Beijing, China.
authors: Congwen
authors: C
authors: Wei
authors: Beijing Institute of Biotechnology, Beijing, China.
authors: Ting
authors: T
authors: Song
authors: Beijing Institute of Biotechnology, Beijing, China.
authors: Zirui
authors: Z
authors: Zheng
authors: Beijing Institute of Biotechnology, Beijing, China.
authors: Kai
authors: K
authors: Guan
authors: Beijing Institute of Biotechnology, Beijing, China.
authors: Yanhong
authors: Y
authors: Zhang
authors: Department of Life Science, Anhui University, Hefei, China.
authors: Buchang
authors: B
authors: Zhang
authors: University of Colorado at Boulder, Boulder, CO, USA.
authors: Xuedong
authors: X
authors: Liu
authors: Center for Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors: Runlin Z
authors: RZ
authors: Ma
authors: Beijing Institute of Biotechnology, Beijing, China. Electronic address: ccao@bmi.ac.cn.
authors: Cheng
authors: C
authors: Cao
authors: Beijing Institute of Biotechnology, Beijing, China. Electronic address: towall@yahoo.com.
authors: Hui
authors: H
authors: Zhong
authors: Beijing Institute of Biotechnology, Beijing, China. Electronic address: xuquanbin73@yahoo.com.
authors: Quanbin
authors: Q
authors: Xu
mesh_terms: D000782:Aneuploidy; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus
chemical_list: D018797:Cell Cycle Proteins; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; C075840:TTK protein, human
keywords: Aneuploidy; Mps1; Overexpression; Spindle assembly checkpoint; Tumorigenesis