title: Overexpression of NADH oxidase gene from Deinococcus geothermalis in Escherichia coli.
abstract: When using stable enzyme genes from a thermophile to create a biosensor in Escherichia coli, it is vital that these genes be overexpressed in order to provide a sufficient supply of enzymes. In this study, overexpression of the NADH oxidase (Nox) gene from the thermophile Deinococcus geothermalis was successfully achieved with the aim of creating a stable biosensor active at room temperatures. To do so, modification of 10 nucleotides, GAAATTAACT, upstream of the start codon of the Nox gene was necessary.
authors: Department of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 3690293, Japan.
authors: Sase
authors: S
authors: Kazuya
authors: Department of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 3690293, Japan.
authors: Iwasaki
authors: I
authors: Tomomi
authors: Department of Life Science and Green Chemistry, Faculty of Engineering, Saitama Institute of Technology, Fukaya 3690293, Japan.
authors: Karasaki
authors: K
authors: Hatsune
authors: Department of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 3690293, Japan; Department of Life Science and Green Chemistry, Faculty of Engineering, Saitama Institute of Technology, Fukaya 3690293, Japan. Electronic address: ishikawa@sit.ac.jp.
authors: Ishikawa
authors: I
authors: Masahide
mesh_terms: D001483:Base Sequence; D034301:Deinococcus / Q000201:enzymology* / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D006639:Histidine / Q000378:metabolism; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000302:isolation & purification; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D009842:Oligopeptides / Q000378:metabolism
chemical_list: C471213:His-His-His-His-His-His; D009097:Multienzyme Complexes; D009842:Oligopeptides; D006639:Histidine; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases
keywords: Deinococcus geothermalis; biosensor; overexpression; stable enzyme