title: Collagenase digestion down-regulates the density of CD27 on lymphocytes.
abstract: Collagenases are widely used for tissue digestion in experiments due to their potent hydrolysis of connective tissue. CD27, also known as tumor necrosis factor receptor superfamily 7 (TNFRSF7), is limited to be expressed on the cells of the lymphoid lineage. In our preliminary research, we found that CD27 on NK cells was almost disappeared with the digestion of type I collagenase for 90min. This phenomenon suggests that the process of tissue digestion may affect the density of CD27 on cells. In order to verify this, the lungs of mice were digested with types I and IV collagenase or grinded, respectively. The percentage of CD27(+) cells and the density of CD27 on cells were assayed by flow cytometry. The data presented that the percentage of CD27(+) cells and the density of CD27 on lymphocytes gradually decreased with the time of digestion with type I or IV collagenase. We also detected that the density of CD11b on NK cells was not affected by collagenase digestion. Collectively, the findings of the present study suggest that the collagenase digestion has a selective effect on the density of molecules on cells.
authors: Department of Immunology, School of Basic Medicine, Tongji Medical College, HuaZhong University of Science and Technology, 430030 Wuhan, China.
authors: Zhangbo
authors: Z
authors: Chen
authors: Department of Immunology, School of Basic Medicine, Tongji Medical College, HuaZhong University of Science and Technology, 430030 Wuhan, China.
authors: Xiangyu
authors: X
authors: Chen
authors: Department of Immunology, School of Basic Medicine, Tongji Medical College, HuaZhong University of Science and Technology, 430030 Wuhan, China.
authors: Yong
authors: Y
authors: Xu
authors: Department of Immunology, School of Basic Medicine, Tongji Medical College, HuaZhong University of Science and Technology, 430030 Wuhan, China.
authors: Ping
authors: P
authors: Xiong
authors: Department of Immunology, School of Basic Medicine, Tongji Medical College, HuaZhong University of Science and Technology, 430030 Wuhan, China.
authors: Min
authors: M
authors: Fang
authors: Department of Immunology, School of Basic Medicine, Tongji Medical College, HuaZhong University of Science and Technology, 430030 Wuhan, China.
authors: Zheng
authors: Z
authors: Tan
authors: Department of Immunology, School of Basic Medicine, Tongji Medical College, HuaZhong University of Science and Technology, 430030 Wuhan, China.
authors: Feili
authors: F
authors: Gong
authors: Department of Immunology, School of Basic Medicine, Tongji Medical College, HuaZhong University of Science and Technology, 430030 Wuhan, China. Electronic address: zhengfangtj@hust.edu.cn.
authors: Fang
authors: F
authors: Zheng
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D017364:Collagenases / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D007527:Isoenzymes / Q000378:metabolism / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D059748:Proteolysis; D013379:Substrate Specificity; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology / Q000378:metabolism*
chemical_list: D039481:CD11b Antigen; D007527:Isoenzymes; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D017364:Collagenases; C417249:collagenase 1
keywords: CD27; Collagenase; Lymphocyte; NK cell