title: Synergic effects of pregabalin-acetaminophen combination in somatic and visceral nociceptive reactivity.
abstract: OBJECTIVE
  The present study investigates the effects of pregabalin (PGB), acetaminophen (ACET) and tenoxicam (TNX) administration in somatic and visceral nociception, using the tail flick test and the writhing test in mice.
  
  
  METHODS
  In the tail flick test, the substances were administered orally and the latency time response was recorded 15, 30, 60, 90 and 120 min after administration. In the writhing test, pain responses were scored every 5 min during a 30-min period after intraperitoneal injection of diluted acetic acid.
  
  
  RESULTS
  Our study demonstrated that oral administration of the combination PGB-ACET resulted in a stronger increase of latency reaction - statistically significant after 15 min compared to TNX and after 30 min compared to PGB in tail flick test. In the writhing test, the combination PGB-ACET, but also PGB-TNX, resulted in a stronger decrease of writhe numbers - statistically significant compared to the effects of the separate administration of each substance. This decrease was more intense in animals treated with the combination PGB-ACET than with PGB-TNX.
  
  
  CONCLUSIONS
  These results suggest an antinociceptive activity which may be a consequence of the synergic action of the substances.
authors: Department of Pharmacology-Algesiology, University of Medicine and Pharmacy 'Grigore T. Popa', Iasi, Romania.
authors: Liliana
authors: L
authors: Mititelu Tartau
authors: Eliza Gratiela
authors: EG
authors: Popa
authors: Raoul Vasile
authors: RV
authors: Lupusoru
authors: Catalina Elena
authors: CE
authors: Lupusoru
authors: Iulian
authors: I
authors: Stoleriu
authors: Lacramioara
authors: L
authors: Ochiuz
mesh_terms: D000082:Acetaminophen / Q000008:administration & dosage / Q000627:therapeutic use*; D019342:Acetic Acid; D000700:Analgesics / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D004338:Drug Combinations; D004357:Drug Synergism; D006358:Hot Temperature; D008297:Male; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000209:etiology; D010894:Piroxicam / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000627:therapeutic use; D000069583:Pregabalin; D005680:gamma-Aminobutyric Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use
chemical_list: D000700:Analgesics; D004338:Drug Combinations; D010894:Piroxicam; D000082:Acetaminophen; D000069583:Pregabalin; D005680:gamma-Aminobutyric Acid; D019342:Acetic Acid; C032801:tenoxicam