title: Genetic control of anastomosis in Podospora anserina.
abstract: We developed a new microscopy procedure to study anastomoses in the model ascomycete Podospora anserina and compared it with the previous method involving the formation of balanced heterokaryons. Both methods showed a good correlation. Heterokaryon formation was less quantifiable, but enabled to observe very rare events. Microscopic analysis evidenced that anastomoses were greatly influence by growth conditions and were severely impaired in the IDC mutants of the PaMpk1, PaMpk2, IDC1 and PaNox1 pathways. Yet some mutants readily formed heterokaryons, albeit with a delay when compared to the wild type. We also identified IDC(821), a new mutant presenting a phenotype similar to the other IDC mutants, including lack of anastomosis. Complete genome sequencing revealed that IDC(821) was affected in the orthologue of the Neurospora crassa So gene known to control anastomosis in several other ascomycetes.
authors: Institut des Energies de Demain (IED), Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris CEDEX 13, France.
authors: Laetitia Chan Ho
authors: LC
authors: Tong
authors: Institut des Energies de Demain (IED), Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris CEDEX 13, France. Electronic address: philippe.silar@univ-paris-diderot.fr.
authors: Philippe
authors: P
authors: Silar
authors: Institut des Energies de Demain (IED), Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris CEDEX 13, France. Electronic address: herve.lalucque@univ-paris-diderot.fr.
authors: Hervé
authors: H
authors: Lalucque
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D025301:Hyphae / Q000235:genetics / Q000502:physiology*; D008853:Microscopy / Q000379:methods; D009154:Mutation; D048468:Podospora / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D005656:Fungal Proteins
keywords: Anastomosis; MAP kinase; NADPH oxidase; Podospora anserina; Signaling pathways