title: Pinus densiflora Sieb. et Zucc. alleviates lipogenesis and oxidative stress during oleic acid-induced steatosis in HepG2 cells.
abstract: Excess accumulation of lipids and oxidative stress in the liver contribute to nonalcoholic fatty liver disease (NAFLD). We hypothesized that Pinus densiflora Sieb. et Zucc. (PSZ) can protect against NAFLD by regulating lipid accumulation and oxidative stress in the liver. To investigate the effect of PSZ upon NAFLD, we used an established cellular model: HepG2 cells treated with oleic acid. Then, the extent of hepatic steatosis and oxidative stress was assessed and levels of inflammatory markers measured. Oleic acid-treated HepG2 cells, compared with controls, had greater lipid accumulation. PSZ decreased lipid accumulation by 63% in oleic acid-treated HepG2 cells. Additionally, PSZ decreased the target gene expression of lipogenesis such as sterol regulatory element binding protein-1c, fatty acid synthase, stearoyl-CoA desaturase-1, diacylglycerol O-acyltransferase-1, and acetyl-CoA carboxylase-1 by 1.75, 6.0, 2.32, 1.93 and 1.81 fold, respectively. In addition, Oleic acid-treated HepG2 cells elicited extensive accumulation of tumor necrosis factor-α (TNFα) by 4.53 fold, whereas PSZ-treated cells decreased the expression of TNFα mRNA by 1.76 fold. PSZ significantly inhibited oxidative stress induced by reactive oxygen species. These results suggest that PSZ has effects on steatosis in vitro and further studies are needed in vivo to verify the current observations.
authors: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea. kwpark@skku.edu.
authors: Yu-Jin
authors: YJ
authors: Hwang
authors: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea. hr226@naver.com.
authors: Hae-Ri
authors: HR
authors: Wi
authors: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea. kimhrr@korea.kr.
authors: Haeng-Ran
authors: HR
authors: Kim
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, Korea. kwpark@skku.edu.
authors: Kye Won
authors: KW
authors: Park
authors: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea. kah366@korea.kr.
authors: Kyung-A
authors: KA
authors: Hwang
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D050155:Lipogenesis / Q000187:drug effects*; D065626:Non-alcoholic Fatty Liver Disease / Q000139:chemically induced / Q000188:drug therapy / Q000517:prevention & control*; D009829:Oleic Acids / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects*; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D009829:Oleic Acids; D010936:Plant Extracts; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D051780:Sterol Regulatory Element Binding Protein 1; D014409:Tumor Necrosis Factor-alpha; D013230:Stearoyl-CoA Desaturase; D064429:Fatty Acid Synthases; C000588423:ACACA protein, human; D000103:Acetyl-CoA Carboxylase