title: Genetic polymorphisms of Interleukin-18 are not associated with allograft function in kidney transplant recipients.
abstract: Interleukin 18 (IL-18) is a proinflammatory cytokine that plays a role in host defense by upregulating both innate and acquired immune responses. Analysis of IL18 polymorphisms may be clinically important since their roles have been recognized in a variety of inflammatory and autoimmune disorders. However, the role of this cytokine polymorphisms in kidney transplant still remains unclear. In this study, we evaluated the associations between IL18 polymorphisms and graft function assessed by creatinine clearance in kidney transplant recipients. A total of 82 kidney transplant recipients and 183 healthy controls were enrolled, and frequencies of alleles, genotypes and haplotypes for IL18 polymorphisms were determined and compared with creatinine clearance. The -607C/A (rs1946518) and -137C/G (rs187238) variant alleles in the IL18 gene were determined by polymerase chain reaction. In our study, no significant association was found between the IL18 variants and creatinine clearance (p > 0.05). Nonetheless, polymorphism analysis revealed an increase in the frequency of the IL18 major haplotype -607C/-137G in kidney transplant patients (odds ratio 2.57, 95% confidence interval 1.45-4.55, p = 0.0014). Finally, we found that IL18 polymorphisms did not influence the renal function and that IL18 haplotype -607C/-137G seems to be associated with kidney transplant recipients.
authors: Departamento de Análises Clínicas e Toxicológicas , Universidade Federal do Rio Grande do Norte , Natal, RN , Brazil .
authors: Wenna Gleyce Araújo
authors: WG
authors: do Nascimento
authors: Departamento de Medicina Clínica , Faculdade de Medicina de Ribeirão Preto , Universidade de São Paulo , Ribeirão Preto, SP , Brazil .
authors: Daiani Alves
authors: DA
authors: Cilião
authors: Programa de Pós-Graduação em Ciências Farmacêuticas , Universidade Federal do Rio Grande do Norte , Natal, RN , Brazil .
authors: Julieta
authors: J
authors: Genre
authors: Departamento de Análises Clínicas e Toxicológicas , Universidade Federal do Rio Grande do Norte , Natal, RN , Brazil .
authors: Dikson Dibe
authors: DD
authors: Gondim
authors: Hemocentro do Rio Grande do Norte , Natal, RN , Brazil .
authors: Renata Gomes
authors: RG
authors: Alves
authors: Departamento de Medicina Clínica , Faculdade de Medicina de Ribeirão Preto , Universidade de São Paulo , Ribeirão Preto, SP , Brazil .
authors: Neife Deghaide
authors: ND
authors: Hassan
authors: Departamento de Patologia , Universidade Federal do Rio Grande do Norte , Natal, RN , Brazil .
authors: Francisco Pignataro
authors: FP
authors: Lima
authors: Departamento de Medicina Integrativa , Universidade Federal do Rio Grande do Norte , Natal, RN , Brazil .
authors: Maurício Galvão
authors: MG
authors: Pereira
authors: Departamento de Medicina Clínica , Faculdade de Medicina de Ribeirão Preto , Universidade de São Paulo , Ribeirão Preto, SP , Brazil .
authors: Eduardo Antônio
authors: EA
authors: Donadi
authors: Departamento de Análises Clínicas e Toxicológicas , Universidade Federal do Rio Grande do Norte , Natal, RN , Brazil .
authors: Janaina Cristiana
authors: JC
authors: de Oliveira Crispim
keywords: -137C/G; -607C/A; IL-18; kidney transplant