title: Alleviation of severe inflammatory responses in LPS-exposed mice by Schisantherin A.
abstract: In this study, we aimed to investigate our hypothesis starting that Schisantherin A (SchA), which exerts significant anti-inflammatory effects in vitro, could reduce the pulmonary inflammatory response in an acute lung injury (ALI) model. ALI was induced in mice by exposure to lipopolysaccharide (LPS, 20 mg/kg), and the inflammatory mediator production, neutrophil infiltration, and histopathological changes were evaluated. SchA at a dose of 100 mg/kg significantly improved survival rate of mice injected with LPS. The levels of TNF-α and IL-6 in bronchoalveolar lavage fluid (BALF) and the histopathological changes due to the injury were significantly inhibited when SchA was administered before or after LPS insult, and the infiltration of neutrophils and macrophages in lung tissues induced by LPS were suppressed by SchA. Additionally, pretreatment with SchA notably blocked the activation of nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinases (MAPKs). Taken together, SchA showed obvious anti-inflammatory effects in an LPS-induced ALI model via blockage of the NF-κB and MAPK pathways. Thus, SchA may be an innovative therapy for inflammatory diseases.
authors: The First Hospital of Jilin University, 71 Xinmin Road, Changchun 130021, PR China.
authors: Dan
authors: D
authors: Li
authors: The First Hospital of Jilin University, 71 Xinmin Road, Changchun 130021, PR China.
authors: Xinxin
authors: X
authors: Ci
authors: The First Hospital of Jilin University, 71 Xinmin Road, Changchun 130021, PR China.
authors: Yang
authors: Y
authors: Li
authors: The First Hospital of Jilin University, 71 Xinmin Road, Changchun 130021, PR China.
authors: Chaoying
authors: C
authors: Liu
authors: The First Hospital of Jilin University, 71 Xinmin Road, Changchun 130021, PR China.
authors: Zhongmei
authors: Z
authors: Wen
authors: The First Hospital of Jilin University, 71 Xinmin Road, Changchun 130021, PR China.
authors: Jing
authors: J
authors: Jie
authors: The First Hospital of Jilin University, 71 Xinmin Road, Changchun 130021, PR China. Electronic address: pengliping1263@163.com.
authors: Liping
authors: L
authors: Peng
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000401:mortality; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002465:Cell Movement / Q000187:drug effects; D034242:Cyclooctanes / Q000494:pharmacology / Q000627:therapeutic use*; D004149:Dioxoles / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D017705:Lignans / Q000494:pharmacology / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020556:Neutrophil Infiltration / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D011654:Pulmonary Edema / Q000188:drug therapy / Q000209:etiology; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D016019:Survival Analysis
chemical_list: D000893:Anti-Inflammatory Agents; D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D008070:Lipopolysaccharides; D008315:Malondialdehyde; C034557:schizandrer A; D009195:Peroxidase; D013482:Superoxide Dismutase
keywords: ALI; LPS; MAPKs; NF-κB; Schisantherin A