title: Localization of excitatory amino acid transporters EAAT1 and EAAT2 in human postmortem cortex: a light and electron microscopic study.
abstract: The process of glutamate release, activity, and reuptake involves the astrocyte, the presynaptic and postsynaptic neurons. Glutamate is released into the synapse and may occupy and activate receptors on both neurons and astrocytes. Glutamate is rapidly removed from the synapse by a family of plasma membrane excitatory amino acid transporters (EAATs), also localized to neurons and astrocytes. The purpose of the present study was to examine EAAT labeling in the postmortem human cortex at the light and electron microscopic (EM) levels. The postmortem prefrontal cortex was processed for EAAT1 and EAAT2 immunohistochemistry. At the light microscopic level, EAAT1 and EAAT2 labeling was found in both gray and white matter. Most cellular labeling was in small cells which were morphologically similar to glia. In addition, EAAT1-labeled neurons were scattered throughout, some of which were pyramidal in shape. At the EM level, EAAT1 and EAAT2 labeling was found in astrocytic soma and processes surrounding capillaries. EAAT labeling was also found in small astrocytic processes adjacent to axon terminals forming asymmetric (glutamatergic) synapses. While EAAT2 labeling was most prevalent in astrocytic processes, EAAT1 labeling was also present in neuronal processes including the soma, axons, and dendritic spines. Expression of EAAT1 protein on neurons may be due to the hypoxia associated with the postmortem interval, and requires further confirmation. The localization of EAATs on the astrocytic plasma membrane and adjacent to excitatory synapses is consistent with the function of facilitating glutamate reuptake and limiting glutamate spillover. Establishment that EAAT1 and EAAT2 can be measured at the EM level in human postmortem tissues will permit testing of hypotheses related to these molecules in diseases lacking analogous animal models.
authors: Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham, Birmingham, AL, USA. Electronic address: rcusidor@uab.edu.
authors: R C
authors: RC
authors: Roberts
authors: Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham, Birmingham, AL, USA.
authors: J K
authors: JK
authors: Roche
authors: Department of Psychiatry and Behavioral Neuroscience, University of Cincinnati College of Medicine, Cincinnati, OH, USA. Electronic address: robert.mccullumsmith@uc.edu.
authors: R E
authors: RE
authors: McCullumsmith
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000648:ultrastructure; D027341:Excitatory Amino Acid Transporter 1 / Q000235:genetics / Q000378:metabolism*; D027342:Excitatory Amino Acid Transporter 2; D005260:Female; D050491:Glutamate Plasma Membrane Transport Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D018345:Mice, Knockout; D008854:Microscopy, Electron; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D017397:Prefrontal Cortex / Q000098:blood supply / Q000378:metabolism* / Q000648:ultrastructure*; D013045:Species Specificity
chemical_list: D027341:Excitatory Amino Acid Transporter 1; D027342:Excitatory Amino Acid Transporter 2; D050491:Glutamate Plasma Membrane Transport Proteins; C493397:SLC1A2 protein, human; C493394:SLC1A3 protein, human; C493393:Slc1a3 protein, mouse
keywords: DAB; EAATs; EM; EtOH; LTD; LTP; N-methyl-d-aspartate; NMDA; PB; PBS; PMI; PSD; astrocytes; diaminobenzidine; electron microscopic; electron microscopy; ethanol; excitatory amino acid transporters; glutamate transporter; long-term depression; long-term potentiation; mitochondria; phosphate buffer; phosphate-buffered saline; postmortem interval; postsynaptic density; rER; rough endoplasmic reticulum; ultrastructure