title: Trichoderma songyi sp. nov., a new species associated with the pine mushroom (Tricholoma matsutake).
abstract: A new species, Trichoderma songyi, was found to be associated with the pine mushroom (Tricholoma matsutake) in Korea. This species was isolated from three different substrates: Tricholoma matsutake basidiomata, as well as roots of Pinus densiflora and soil in the fairy ring. Based on its molecular and phenotypic characteristics, we demonstrate that Trichoderma songyi is unique and distinguishable from closely related species. We performed phylogenetic analyses based on two molecular markers, the genes for both translation elongation factor 1-alpha and the second largest subunit of RNA polymerase II. Phylogenetic analyses showed that Trichoderma songyi is closely related to Trichoderma koningii aggregate and Trichoderma caerulescens. Morphologically, Trichoderma songyi can be distinguished from these closely related taxa by its growth rates, colony morphology on PDA in darkness, and coconut-like odour. Due to the economic importance of the pine mushroom, the relationship between Trichoderma songyi and Tricholoma matsutake should be studied further.
authors: School of Biological Sciences, Seoul National University, Seoul, 151-747, South Korea.
authors: Myung Soo
authors: MS
authors: Park
authors: Seung-Yoon
authors: SY
authors: Oh
authors: Hae Jin
authors: HJ
authors: Cho
authors: Jonathan J
authors: JJ
authors: Fong
authors: Woo-Jae
authors: WJ
authors: Cheon
authors: Young Woon
authors: YW
authors: Lim
mesh_terms: D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D007723:Korea; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D028223:Pinus / Q000382:microbiology*; D012319:RNA Polymerase II / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012988:Soil Microbiology; D014242:Trichoderma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D055439:Tricholoma
chemical_list: D004271:DNA, Fungal; D020648:Peptide Elongation Factor 1; D012319:RNA Polymerase II