title: Generation of an allelic series of knock-in mice using recombinase-mediated cassette exchange (RMCE).
abstract: Molecular genetic strategies applying embryonic stem cell (ES cell) technologies to study the function of a gene in mice or to generate a mouse model for a human disease are continuously under development. Next to (conditional) inactivation of genes the application and importance of approaches to generate knock-in mutations are increasing. In this chapter the principle and application of recombinase-mediated cassette exchange (RMCE) are discussed as being a new emerging knock-in strategy, which enables easy generation of a series of different knock-in mutations within one gene. An RMCE protocol, which was used to generate a series of different knock-in mutations in the Lrp1 gene of ES cells, is described in detail as an example of how RMCE can be used to generate highly efficiently an allelic series of differently modified ES cell clones from a parental modified ES cell clone. Subsequently the differently modified ES cell clones can be used to generate an allelic series of mutant knock-in mice.
authors: Laboratory for Experimental Mouse Genetics, Center for Human Genetics, KU Leuven, Campus Gasthuisberg, Herestraat 49, 602, 3000, Leuven, Belgium, anton.roebroek@med.kuleuven.be.
authors: Anton J M
authors: AJ
authors: Roebroek
authors: Bart
authors: B
authors: Van Gool
mesh_terms: D000483:Alleles; D000818:Animals; D002460:Cell Line; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000378:metabolism; D055879:Gene Knock-In Techniques / Q000379:methods*; D019426:Integrases / Q000378:metabolism; D051379:Mice; D016254:Mutagenesis, Insertional / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D045522:Recombinases / Q000378:metabolism*
chemical_list: D045522:Recombinases; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; D019426:Integrases