title: Response surface methodology to optimise Accelerated Solvent Extraction of steviol glycosides from Stevia rebaudiana Bertoni leaves.
abstract: Following the approval of steviol glycosides as a food additive in Europe in December 2011, large-scale stevia cultivation will have to be developed within the EU. Thus there is a need to increase the efficiency of stevia evaluation through germplasm enhancement and agronomic improvement programs. To address the need for faster and reproducible sample throughput, conditions for automated extraction of dried stevia leaves using Accelerated Solvent Extraction were optimised. A response surface methodology was used to investigate the influence of three factors: extraction temperature, static time and cycle number on the stevioside and rebaudioside A extraction yields. The model showed that all the factors had an individual influence on the yield. Optimum extraction conditions were set at 100 °C, 4 min and 1 cycle, which yielded 91.8% ± 3.4% of total extractable steviol glycosides analysed. An additional optimisation was achieved by reducing the grind size of the leaves giving a final yield of 100.8% ± 3.3%.
authors: Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), 4 Allée Emile Monso, F-31030 Toulouse, France; INRA, UMR 1010 CAI, F-31030 Toulouse, France. Electronic address: jeanbaptiste.jentzer@ensiacet.fr.
authors: Jean-Baptiste
authors: JB
authors: Jentzer
authors: Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), 4 Allée Emile Monso, F-31030 Toulouse, France; INRA, UMR 1010 CAI, F-31030 Toulouse, France. Electronic address: marion.alignan@ensiacet.fr.
authors: Marion
authors: M
authors: Alignan
authors: Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), 4 Allée Emile Monso, F-31030 Toulouse, France; INRA, UMR 1010 CAI, F-31030 Toulouse, France; King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: carlos.vacagarcia@ensiacet.fr.
authors: Carlos
authors: C
authors: Vaca-Garcia
authors: Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), 4 Allée Emile Monso, F-31030 Toulouse, France; INRA, UMR 1010 CAI, F-31030 Toulouse, France. Electronic address: luc.rigal@ensiacet.fr.
authors: Luc
authors: L
authors: Rigal
authors: Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro-industrielle), 4 Allée Emile Monso, F-31030 Toulouse, France; INRA, UMR 1010 CAI, F-31030 Toulouse, France. Electronic address: gerard.vilarem@ensiacet.fr.
authors: Gérard
authors: G
authors: Vilarem
mesh_terms: D002851:Chromatography, High Pressure Liquid; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification*; D005060:Europe; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D008962:Models, Theoretical; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D036625:Stevia / Q000737:chemistry*
chemical_list: D045786:Diterpenes, Kaurane; D005960:Glucosides; D010936:Plant Extracts; D012997:Solvents; C012043:stevioside; C012042:steviol
keywords: Accelerated Solvent Extraction; Genotype screening; HPLC; Response surface methodology; Stevia rebaudiana Bertoni; Steviol glycosides