title: FGF10: Type III Epithelial Mesenchymal Transition and Invasion in Breast Cancer Cell Lines.
abstract: OBJECTIVE
  Fibroblastic growth factor-10 (FGF-10) has an important role in type I epithelial mesenchymal transition (EMT) during the embryonic period of life (gastrulation). Since EMT has a critical role during cancer cells invasion and metastasis (type III) this study sought to investigate the possible role of FGF-10 in type III EMT by monitoring breast cancer cell lines' behavior by FGF-10 regulation.
  
  
  METHODS
  MCF-7 and MDA-MB-231 cell lines with different levels of FGF10 expression were treated with FGF-10 recombinant protein and FGF-10 siRNA, respectively.
  
  
  RESULTS
  The cell viability, migration, colony formation and wound healing have a direct relationship with FGF-10 expression, while FGF-10 expression decreased apoptosis. All mesenchymal factors (such as vimentin, N cadherin, snail, slug, TGF-β) increased due to FGF-10 expression with contrary expression of epithelial markers (such as E-cadherin). Moreover, GSK3β phosphorylation (inactivation) increased with FGF-10 expression.
  
  
  CONCLUSIONS
  The important role of FGF-10 in type III EMT on cancer cells and initiation of metastasis via various kinds of signaling pathways has been suggested.
authors: 1. Group of Genetics, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences (TUMS), Tehran, Iran ; 2. Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.
authors: Ali
authors: A
authors: Abolhassani
authors: 1. Group of Genetics, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors: Gholam Hossein
authors: GH
authors: Riazi
authors: 2. Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.
authors: Ebrahim
authors: E
authors: Azizi
authors: 3. Group of experimental research in cancer, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors: Saeid
authors: S
authors: Amanpour
authors: 3. Group of experimental research in cancer, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors: Samad
authors: S
authors: Muhammadnejad
authors: 3. Group of experimental research in cancer, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors: Mahnaz
authors: M
authors: Haddadi
authors: 4. Department of Genetics, Faculty of Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors: Ali
authors: A
authors: Zekri
authors: 1. Group of Genetics, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
authors: Reza
authors: R
authors: Shirkoohi
keywords: FGF10; breast cancer; epithelial mesnchymal transition; invasion; metastasis.