title: Effects of deferoxamine on the repair ability of dental pulp cells in vitro.
abstract: BACKGROUND
  In previous studies, we found that hypoxia promoted the mineralization of dental pulp cells (DPCs). However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia. The purpose of this study was to investigate the effects of DFO on the repair ability of DPCs.
  
  
  METHODS
  DPCs were obtained by using a tissue explant technique in vitro and were treated with different concentrations of DFO or hypoxia culture for 2 days. The viability, proliferation, migration, and odontogenic differentiation of DPCs were assayed and analyzed. The expression of hypoxia-inducible factor 1-alpha (HIF-1α) was assessed through Western blotting.
  
  
  RESULTS
  Ten micromolars of DFO enhanced the expression of HIF-1α similarly to hypoxia and did not affect the viability of DPCs for 2 days. Furthermore, the proliferation, migration, and odontogenic differentiation of DPCs were promoted by DFO.
  
  
  CONCLUSIONS
  These results suggest that DFO might improve the repair ability of DPCs by HIF-1α.
authors: Department of General Dentistry, 9th People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Long
authors: L
authors: Jiang
authors: Department of General Dentistry, 9th People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Wei-Wei
authors: WW
authors: Peng
authors: Department of General Dentistry, 9th People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Li-Fen
authors: LF
authors: Li
authors: Department of General Dentistry, 9th People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Rong
authors: R
authors: Du
authors: Department of General Dentistry, 9th People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Tian-Tian
authors: TT
authors: Wu
authors: Department of General Dentistry, 9th People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Zhuo-Jun
authors: ZJ
authors: Zhou
authors: Department of General Dentistry, 9th People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Jun-Jun
authors: JJ
authors: Zhao
authors: Department of General Dentistry, 9th People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Ya
authors: Y
authors: Yang
authors: Department of General Dentistry, 9th People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors: Dong-Lin
authors: DL
authors: Qu
authors: Department of General Dentistry, 9th People's Hospital, Shanghai JiaoTong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China. Electronic address: zyq1590@163.com.
authors: Ya-Qin
authors: YQ
authors: Zhu
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000032:analysis; D002113:Calcification, Physiologic / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D003676:Deferoxamine / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D005456:Fluorescent Dyes; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis; D009804:Odontoblasts / Q000187:drug effects; D015675:Osteocalcin / Q000032:analysis; D012038:Regeneration / Q000187:drug effects; D017262:Siderophores / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D055815:Young Adult
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D017262:Siderophores; D013778:Tetrazolium Salts; D013844:Thiazoles; D015675:Osteocalcin; D000469:Alkaline Phosphatase; C022616:thiazolyl blue; D003676:Deferoxamine
keywords: Deferoxamine; dental pulp cells; hypoxia-inducible factors-1α; repair ability