title: Quantitative comparison of tumor delivery for multiple targeted nanoparticles simultaneously by multiplex ICP-MS.
abstract: Given the rapidly expanding library of disease biomarkers and targeting agents, the number of unique targeted nanoparticles is growing exponentially. The high variability and expense of animal testing often makes it unfeasible to examine this large number of nanoparticles in vivo. This often leads to the investigation of a single formulation that performed best in vitro. However, nanoparticle performance in vivo depends on many variables, many of which cannot be adequately assessed with cell-based assays. To address this issue, we developed a lanthanide-doped nanoparticle method that allows quantitative comparison of multiple targeted nanoparticles simultaneously. Specifically, superparamagnetic iron oxide (SPIO) nanoparticles with different targeting ligands were created, each with a unique lanthanide dopant. Following the simultaneous injection of the various SPIO compositions into tumor-bearing mice, inductively coupled plasma mass spectroscopy was used to quantitatively and orthogonally assess the concentration of each SPIO composition in serial blood and resected tumor samples.
authors: 1] Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia PA, 19104 USA [2].
authors: Andrew
authors: A
authors: Elias
authors: 1] Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia PA, 19104 USA [2].
authors: Samuel H
authors: SH
authors: Crayton
authors: Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia PA, 19104 USA.
authors: Robert
authors: R
authors: Warden-Rothman
authors: Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia PA, 19104 USA.
authors: Andrew
authors: A
authors: Tsourkas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry; D015870:Gene Expression; D050958:HSP47 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D055166:Hepatic Stellate Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015552:Injections, Intralesional; D039482:Integrin alpha5 / Q000235:genetics / Q000378:metabolism*; D028581:Lanthanoid Series Elements / Q000737:chemistry; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D058990:Molecular Targeted Therapy; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D013054:Spectrophotometry, Atomic; D023041:Xenograft Model Antitumor Assays
chemical_list: D000906:Antibodies; D005290:Ferric Compounds; D050958:HSP47 Heat-Shock Proteins; D039482:Integrin alpha5; D028581:Lanthanoid Series Elements; D010455:Peptides; D018719:Erb-b2 Receptor Tyrosine Kinases; C494660:SERPINH1 protein, human; C000499:ferric oxide; C524056:Erbb2 protein, mouse