title: New technique to isolate anthocyanins from Delaware grapes by forming an aluminium complex using a Discovery DPA-6S.
abstract: An aluminium complex of crude Delaware grape anthocyanins, bearing an ortho-dihydroxyl group on the B ring and/or a p-coumaroyl group as an ester with flavocommelin, was formed and isolated by ethanol precipitation. Using a Discovery DPA-6S short column, selected anthocyanins were isolated to give cyanidin 3-glucoside (Cy3G, 48.2% yield with 95.2% purity) and cyanidin 3-(6-O-p-coumaroylglucoside) (Cy3-pC·G, 44.9% yield with 91.4% purity) from natural Delaware grape skin extracts without ODS-HPLC column chromatography. DPPH radical scavenging activity of the complex pigment was 3.4 ± 0.10 μmol TE/mg. Moreover, isolated pure anthocyanins from the complex pigment showed significantly higher DPPH radical scavenging activity [4.5 ± 0.08 μmol TE/mg (Cy3G) and 4.5 ± 0.04 μmol TE/mg (Cy3-pC·G), (p<0.05)].
authors: The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan.
authors: Takayuki
authors: T
authors: Asada
authors: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
authors: Yoriko
authors: Y
authors: Koi
authors: The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan; Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan. Electronic address: tamura@ag.kagawa-u.ac.jp.
authors: Hirotoshi
authors: H
authors: Tamura
mesh_terms: D000535:Aluminum / Q000032:analysis*; D000872:Anthocyanins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D027843:Vitis / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D000535:Aluminum
keywords: Acylated anthocyanidin 3-glucoside; Anthocyanidin 3-glucoside; Complex pigment; Selective isolation