title: An approach to identify SNPs in the gene encoding acetyl-CoA acetyltransferase-2 (ACAT-2) and their proposed role in metabolic processes in pig.
abstract: The novel liver protein acetyl-CoA acetyltransferase-2 (ACAT2) is involved in the beta-oxidation and lipid metabolism. Its comprehensive relative expression, in silico non-synonymous single nucleotide polymorphism (nsSNP) analysis, as well as its annotation in terms of metabolic process with another protein from the same family, namely, acetyl-CoA acyltransferase-2 (ACAA2) was performed in Sus scrofa. This investigation was conducted to understand the most important nsSNPs of ACAT2 in terms of their effects on metabolic activities and protein conformation. The two most deleterious mutations at residues 122 (I to V) and 281 (R to H) were found in ACAT2. Validation of expression of genes in the laboratory also supported the idea of differential expression of ACAT2 and ACAA2 conceived through the in silico analysis. Analysis of the relative expression of ACAT2 and ACAA2 in the liver tissue of Jeju native pig showed that the former expressed significantly higher (P<0.05). Overall, the computational prediction supported by wet laboratory analysis suggests that ACAT2 might contribute more to metabolic processes than ACAA2 in swine. Further associations of SNPs in ACAT2 with production traits might guide efforts to improve growth performance in Jeju native pigs.
authors: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju-si, Jeju-do, South Korea.
authors: Simrinder Singh
authors: SS
authors: Sodhi
authors: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju-si, Jeju-do, South Korea.
authors: Mrinmoy
authors: M
authors: Ghosh
authors: The Animal Genomics and Breeding Center, Hankyong National University, Anseong-si, Gyeonggi-do, South Korea.
authors: Ki Duk
authors: KD
authors: Song
authors: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju-si, Jeju-do, South Korea.
authors: Neelesh
authors: N
authors: Sharma
authors: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju-si, Jeju-do, South Korea.
authors: Jeong Hyun
authors: JH
authors: Kim
authors: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju-si, Jeju-do, South Korea.
authors: Nam Eun
authors: NE
authors: Kim
authors: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Kangwon National University, Chuncheon, South Korea.
authors: Sung Jin
authors: SJ
authors: Lee
authors: Department of Mechanical and System Engineering, College of Engineering, Jeju National University, Jeju-si, Jeju-do, South Korea.
authors: Chul Woong
authors: CW
authors: Kang
authors: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju-si, Jeju-do, South Korea.
authors: Sung Jong
authors: SJ
authors: Oh
authors: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju-si, Jeju-do, South Korea; Sustainable Agriculture Research Institute (SARI), Jeju National University, Jeju-si, Jeju-do, South Korea.
authors: Dong Kee
authors: DK
authors: Jeong
mesh_terms: D000102:Acetyl-CoA C-Acyltransferase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics; D004795:Enzyme Stability; D015870:Gene Expression; D063990:Gene Ontology; D056726:Genetic Association Studies; D060005:Genotyping Techniques; D008099:Liver / Q000201:enzymology; D058977:Molecular Sequence Annotation; D020641:Polymorphism, Single Nucleotide; D002785:Sterol O-Acyltransferase / Q000235:genetics* / Q000378:metabolism; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development; D000097587:Sterol O-Acyltransferase 2
chemical_list: D000102:Acetyl-CoA C-Acyltransferase; D002785:Sterol O-Acyltransferase; D000097587:Sterol O-Acyltransferase 2