title: Monoclonal antibody targeting chikungunya virus envelope 1 protein inhibits virus release.
abstract: Chikungunya virus (CHIKV) causes an acute clinical illness characterized by sudden high fever, intense joint pain, and skin rash. Recent outbreaks of chikungunya disease in Africa and Asia are a major public health concern; however, there is currently no effective licensed vaccine or specific treatment. This study reported the development of a mouse monoclonal antibody (MAb), CK47, which recognizes domain III within the viral envelope 1 protein and inhibited the viral release process, thereby preventing the production of progeny virus. The MAb had no effect on virus entry and replication processes. Thus, CK47 may be a useful tool for studying the mechanisms underlying CHIKV release and may show potential as a therapeutic agent.
authors: Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok 10400, Thailand.
authors: Promsin
authors: P
authors: Masrinoul
authors: Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok 10400, Thailand.
authors: Orapim
authors: O
authors: Puiprom
authors: Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors: Atsushi
authors: A
authors: Tanaka
authors: Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok 10400, Thailand; Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors: Miwa
authors: M
authors: Kuwahara
authors: Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok 10400, Thailand.
authors: Panjaporn
authors: P
authors: Chaichana
authors: Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors: Kazuyoshi
authors: K
authors: Ikuta
authors: Center of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
authors: Pongrama
authors: P
authors: Ramasoota
authors: Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok 10400, Thailand; Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: tamaoka@biken.osaka-u.ac.jp.
authors: Tamaki
authors: T
authors: Okabayashi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000914:Antibodies, Viral / Q000276:immunology / Q000494:pharmacology*; D065632:Chikungunya Fever / Q000188:drug therapy* / Q000821:virology; D002646:Chikungunya virus / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D014759:Viral Envelope Proteins / Q000276:immunology*; D053586:Virus Internalization / Q000187:drug effects; D057074:Virus Release / Q000187:drug effects*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014759:Viral Envelope Proteins
keywords: Chikungunya virus; Monoclonal antibody; Post-entry inhibitory step