title: Biocompatible nanocrystalline natural bonelike carbonated hydroxyapatite synthesized by mechanical alloying in a record minimum time.
abstract: Single phase nanocrystalline biocompatible A-type carbonated hydroxyapatite (A-cHAp) powder has been synthesized by mechanical alloying the stoichiometric mixture of CaCO3 and CaHPO4.2H2O powders in open air at room temperature within 2h of milling. The A-type carbonation in HAp is confirmed by FTIR analysis. Structural and microstructure parameters of as-milled powders are obtained from both Rietveld's powder structure refinement analysis and transmission electron microscopy. Size and lattice strain of nanocrystalline HAp particles are found to be anisotropic in nature. Mechanical alloying causes amorphization of a part of crystalline A-cHAp which is analogous to native bone mineral. Some primary bond lengths of as-milled samples are critically measured. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay test reveals high percentage of cell viability and hence confirms the biocompatibility of the sample. The overall results indicate that the processed A-cHAp has a chemical composition very close to that of biological apatite.
authors: Materials Science Division, Department of Physics, The University of Burdwan, Golapbag, Burdwan-713104, West Bengal, India.
authors: S
authors: S
authors: Lala
authors: Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata-700 032, India.
authors: S
authors: S
authors: Brahmachari
authors: Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata-700 032, India.
authors: P K
authors: PK
authors: Das
authors: UGC-DAE Consortium for Scientific Research, Kolkata-700098, India.
authors: D
authors: D
authors: Das
authors: Department of Materials Science, Indian Association for the Cultivation of Science, Jadavpur, Kolkata-700032 , India.
authors: T
authors: T
authors: Kar
authors: Materials Science Division, Department of Physics, The University of Burdwan, Golapbag, Burdwan-713104, West Bengal, India. Electronic address: skp_bu@yahoo.com.
authors: S K
authors: SK
authors: Pradhan
mesh_terms: D000497:Alloys; D000818:Animals; D016466:CHO Cells; D002254:Carbonates / Q000737:chemistry* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D017886:Durapatite / Q000737:chemistry* / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D014961:X-Ray Diffraction
chemical_list: D000497:Alloys; D002254:Carbonates; D017886:Durapatite
keywords: Hydroxyapatite; MTT assay; Nanoparticles; Rietveld analysis; TEM; XRD