title: Effects of liposome-encapsulated hemoglobin on learning ability in tokai high-avoider rat after total brain ischemia and reperfusion.
abstract: Liposome-encapsulated hemoglobin with low O2 -affinity (l-LEH) was shown to be protective in focal brain ischemia and reperfusion (I/R) in rats and primates. We tested l-LEH in the transient whole brain ischemia in the Tokai high-avoider rat (THA), which has been selected, mated, and bred over 77 generations for a high and consistent learning ability determined by the Sidman avoidance test (SAT). Young/naïve (before SAT) and adult/parent (after SAT) THA rats underwent acute and complete four-vessel occlusion in the chest for 3 or 5 min, administration of 2 mL/kg of l-LEH, saline, or homologous washed red blood cells (RBCs), reperfusion, and resuscitation. One week later, all rats underwent SAT, open-field behavioral observation, Morris water maze tests, and morphological study. Whereas young/naïve rats treated with l-LEH retained a rapid and consistent learning curve as in nonischemic controls, THA rats treated with RBCs or saline had retarded learning response on SAT as well as reduced cellularity in the amygdala. Adult/parent rats with established memory on SAT maintained perfect achievement even after I/R. In contrast, l-LEH-treated rats showed no better performance on Morris water maze (function) or cellularity of the CA1 sector of the hippocampus (morphology) compared with the rats treated with RBCs. Although task performance on SAT and Morris water maze appeared antithetical, morphological observations corresponded to the respective functions, suggesting that l-LEH was protective only for the amygdala on SAT tasks but not for the CA1 sector of the hippocampus on spatial orientation as in our previous studies on focal brain I/R, where the cortex was preserved better than basal ganglia.
authors: Department of Cell Transplantation and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
authors: Akira T
authors: AT
authors: Kawaguchi
authors: Hitoshi
authors: H
authors: Endo
authors: Hiroyuki
authors: H
authors: Aikawa
authors: Mariko
authors: M
authors: Yamano
authors: Yo
authors: Y
authors: Kawaguchi
authors: Munetaka
authors: M
authors: Haida
authors: Tetsu
authors: T
authors: Watanabe
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology* / Q000627:therapeutic use; D002545:Brain Ischemia / Q000188:drug therapy*; D004195:Disease Models, Animal; D006454:Hemoglobins / Q000494:pharmacology* / Q000627:therapeutic use; D008081:Liposomes / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D018782:Maze Learning / Q000187:drug effects*; D051381:Rats; D015424:Reperfusion; D015427:Reperfusion Injury / Q000188:drug therapy*
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes
keywords: Behavior; Established memory; Fresh memory; Hippocampus; Liposome-encapsulated hemoglobin; Reperfusion; Resuscitation; Water maze test; Whole brain ischemia