title: Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation.
abstract: BACKGROUND
  Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in different tissues. ADAM17 and ADAM10 are two members of this family surface metalloproteases involved in germ cell apoptosis during the first wave of spermatogenesis in the rat, but they have other signaling functions in somatic tissues.
  
  
  RESULTS
  In an attempt to further study these two enzymes, we describe the presence and localization in adult male rats. Results showed that both enzymes are detected in germ and Sertoli cells during all the stages of spermatogenesis. Interestingly their protein levels and cell surface localization in adult rats were stage-specific, suggesting activation of these enzymes at particular events of rat spermatogenesis.
  
  
  CONCLUSIONS
  Therefore, these results show that ADAM10 and ADAM17 protein levels and subcellular (cell surface) localization are regulated during rat spermatogenesis.
authors: Paulina
authors: P
authors: Urriola-Muñoz
authors: Carlos
authors: C
authors: Lizama
authors: Raúl
authors: R
authors: Lagos-Cabré
authors: Juan G
authors: JG
authors: Reyes
authors: Ricardo D
authors: RD
authors: Moreno
mesh_terms: D051722:ADAM Proteins / Q000032:analysis / Q000378:metabolism*; D000072197:ADAM10 Protein; D000072198:ADAM17 Protein; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012671:Seminiferous Tubules / Q000737:chemistry; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism; D013087:Spermatids / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000033:anatomy & histology; D019014:fas Receptor / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D019014:fas Receptor; D051722:ADAM Proteins; D000072197:ADAM10 Protein; C542371:ADAM10 protein, rat; D000072198:ADAM17 Protein; C000606464:Adam17 protein, rat