title: Enhanced response of a proteinase K-based conductometric biosensor using nanoparticles.
abstract: Proteinases are involved in a multitude of important physiological processes, such as protein metabolism. For this reason, a conductometric enzyme biosensor based on proteinase K was developed using two types of nanoparticles (gold and magnetic). The enzyme was directly adsorbed on negatively charged nanoparticles and then deposited and cross-linked on a planar interdigitated electrode (IDE). The biosensor was characterized with bovine serum albumin (BSA) as a standard protein. Higher sensitivity was obtained using gold nanoparticles. The linear range for BSA determination was then from 0.5 to 10 mg/L with a maximum response of 154 µs. These results are greater than that found without any nanoparticles (maximum response of 10 µs). The limit of detection (LOD) was 0.3 mg/L. An inter-sensor reproducibility of 3.5% was obtained.
authors: Institute of Analytical Sciences, University of Lyon, La Doua Street, 5, 69622 Villeurbanne, France. widednouira@yahoo.fr.
authors: Wided
authors: W
authors: Nouira
authors: Laboratory of Interfaces and Advanced Materials, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia. abderrazak.maaref@fsm.rnu.tn.
authors: Abderrazak
authors: A
authors: Maaref
authors: University of Lyon, CNRS, UMR 5007, LAGEP-CPE, 43 Bd. 11 Novembre 1918, Villeurbanne, France. elaissari@lagep.univ-lyon1.fr.
authors: Hamid
authors: H
authors: Elaissari
authors: Hubert Curien Laboratory, University of Lyon, F-42023 Saint-Etienne, France. francis.vocanson@univ-st-etienne.fr.
authors: Francis
authors: F
authors: Vocanson
authors: LASC, ISEA, University of Metz, 7 Marconi Street, 57070 Metz, France. maryam.siadat@univ-lorraine.fr.
authors: Maryam
authors: M
authors: Siadat
authors: Institute of Analytical Sciences, University of Lyon, La Doua Street, 5, 69622 Villeurbanne, France. nicole.jaffrezic@univ-lyon1.fr.
authors: Nicole
authors: N
authors: Jaffrezic-Renault
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D003217:Conductometry / Q000295:instrumentation* / Q000379:methods*; D019286:Endopeptidase K / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D008280:Magnetics / Q000295:instrumentation / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D012710:Serum Albumin, Bovine; D006046:Gold; D019286:Endopeptidase K