title: Functional and pharmacological analysis of cardiomyocytes differentiated from human peripheral blood mononuclear-derived pluripotent stem cells.
abstract: Advances in induced pluripotent stem cell (iPSC) technology have set the stage for routine derivation of patient- and disease-specific human iPSC-cardiomyocyte (CM) models for preclinical drug screening and personalized medicine approaches. Peripheral blood mononuclear cells (PBMCs) are an advantageous source of somatic cells because they are easily obtained and readily amenable to transduction. Here, we report that the electrophysiological properties and pharmacological responses of PBMC-derived iPSC CM are generally similar to those of iPSC CM derived from other somatic cells, using patch-clamp, calcium transient, and multielectrode array (MEA) analyses. Distinct iPSC lines derived from a single patient display similar electrophysiological features and pharmacological responses. Finally, we demonstrate that human iPSC CMs undergo acute changes in calcium-handling properties and gene expression in response to rapid electrical stimulation, laying the foundation for an in-vitro-tachypacing model system for the study of human tachyarrhythmias.
authors: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors: Michael
authors: M
authors: Riedel
authors: Division of Pediatric Cardiology, University of Utah School of Medicine, Salt Lake City, UT 83113, USA ; Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
authors: Chuanchau J
authors: CJ
authors: Jou
authors: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors: Shuping
authors: S
authors: Lai
authors: Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
authors: Robert L
authors: RL
authors: Lux
authors: Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
authors: Alonso P
authors: AP
authors: Moreno
authors: Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
authors: Kenneth W
authors: KW
authors: Spitzer
authors: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors: Elizabeth
authors: E
authors: Christians
authors: Division of Pediatric Cardiology, University of Utah School of Medicine, Salt Lake City, UT 83113, USA ; Nora Eccles Harrison CVRTI, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
authors: Martin
authors: M
authors: Tristani-Firouzi
authors: Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors: Ivor J
authors: IJ
authors: Benjamin
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004594:Electrophysiology; D005434:Flow Cytometry; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D059785:Karyotype; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000166:cytology*