title: Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration.
abstract: Polarized cell migration is essential for normal organism development and is also a critical component of cancer cell invasion and disease progression. Directional cell motility requires the coordination of dynamic cell-extracellular matrix interactions as well as repositioning of the Golgi apparatus, both of which can be controlled by the microtubule (MT) cytoskeleton. In this paper, we have identified a new and conserved role for the focal adhesion scaffold protein paxillin in regulating the posttranslational modification of the MT cytoskeleton through an inhibitory interaction with the α-tubulin deacetylase HDAC6. We also determined that through HDAC6-dependent regulation of the MT cytoskeleton, paxillin regulates both Golgi organelle integrity and polarized cell invasion and migration in both three-dimensional and two-dimensional matrix microenvironments. Importantly, these data reveal a fundamental role for paxillin in coordinating MT acetylation-dependent cell polarization and migration in both normal and transformed cells.
authors: Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY 13210.
authors: Nicholas O
authors: NO
authors: Deakin
authors: Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY 13210 turnerce@upstate.edu.
authors: Christopher E
authors: CE
authors: Turner
mesh_terms: D000107:Acetylation; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D016764:Cell Polarity; D005109:Extracellular Matrix / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure*; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D041681:NIH 3T3 Cells; D051419:Paxillin / Q000502:physiology*; D011499:Protein Processing, Post-Translational
chemical_list: C496061:PXN protein, human; D051419:Paxillin; C433811:HDAC6 protein, human; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases