title: Alterations in gene expression during radiation-induced mitotic catastrophe in HeLa Hep2 cells.
abstract: OBJECTIVE
  To explore kinetic changes in the gene expression profile during radiation-induced mitotic catastrophes.
  
  
  METHODS
  Gene expression changes were measured in HPV-infected HeLa Hep2 tumor cells following exposure to 5 Gy of ionizing radiation ((60)Co). Signaling pathways were explored and correlated to the biological responses linked to mitotic catastrophe.
  
  
  RESULTS
  Following irradiation a transient G2-arrest was induced. Anaphase bridge formation and centrosome hyperamplification was observed. These phenotypical changes correlated well with the observed gene expression changes. Genes with altered expression were found to be involved in mitotic processes as well as G2- and spindle assembly checkpoints. Also centrosome-associated genes displayed an increased expression.
  
  
  CONCLUSIONS
  This study elucidates specific characteristics in the altered gene expression pattern induced by irradiation, which can be correlated to the events of mitotic catastrophe in HeLa Hep2 cells. Therapeutic strategies modulating these alterations might potentiate future therapy and enhance tumor cell killing.
authors: Department of Clinical Microbiology, Immunology, Umeå University, Umeå, Sweden.
authors: Theres
authors: T
authors: Lindgren
authors: Department of Clinical Microbiology, Immunology, Umeå University, Umeå, Sweden.
authors: Torgny
authors: T
authors: Stigbrand
authors: Department of Radiation Sciences, Radiation Physics, Umeå University, Umeå, Sweden.
authors: Lennart
authors: L
authors: Johansson
authors: Department of Radiation Sciences, Diagnostic Radiology, Umeå University, Umeå, Sweden.
authors: Katrine
authors: K
authors: Riklund
authors: Department of Clinical Microbiology, Immunology, Umeå University, Umeå, Sweden david.eriksson@climi.umu.se.
authors: David
authors: D
authors: Eriksson
mesh_terms: D018385:Centrosome / Q000528:radiation effects; D059565:G2 Phase Cell Cycle Checkpoints / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000528:radiation effects*; D059467:Transcriptome / Q000528:radiation effects*
keywords: Radiation; cell cycle checkpoint; cell death; gene expression; mitotic catastrophe