title: Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile.
abstract: OBJECTIVE
  The aim of the study was to evaluate the use of global and gene-specific DNA methylation changes as potential biomarkers for gallbladder cancer (GBC) in a cohort from Chile.
  
  
  METHODS
  DNA methylation was analyzed through an ELISA-based technique and quantitative methylation-specific PCR.
  
  
  RESULTS
  Global DNA Methylation Index (p = 0.02) and promoter methylation of SSBP2 (p = 0.01) and ESR1 (p = 0.05) were significantly different in GBC when compared with cholecystitis. Receiver curve operator analysis revealed promoter methylation of APC, CDKN2A, ESR1, PGP9.5 and SSBP2, together with the Global DNA Methylation Index, had 71% sensitivity, 95% specificity, a 0.97 area under the curve and a positive predictive value of 90%.
  
  
  CONCLUSIONS
  Global and gene-specific DNA methylation may be useful biomarkers for GBC clinical assessment.
authors: Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.
authors: Luciane Tsukamoto
authors: LT
authors: Kagohara
authors: Juliana L
authors: JL
authors: Schussel
authors: Tejaswini
authors: T
authors: Subbannayya
authors: Nandini
authors: N
authors: Sahasrabuddhe
authors: Cynthia
authors: C
authors: Lebron
authors: Mariana
authors: M
authors: Brait
authors: Leonel
authors: L
authors: Maldonado
authors: Blanca L
authors: BL
authors: Valle
authors: Francesca
authors: F
authors: Pirini
authors: Martha
authors: M
authors: Jahuira
authors: Jaime
authors: J
authors: Lopez
authors: Pablo
authors: P
authors: Letelier
authors: Priscilla
authors: P
authors: Brebi-Mieville
authors: Carmen
authors: C
authors: Ili
authors: Akhilesh
authors: A
authors: Pandey
authors: Aditi
authors: A
authors: Chatterjee
authors: David
authors: D
authors: Sidransky
authors: Rafael
authors: R
authors: Guerrero-Preston
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D002677:Chile; D002764:Cholecystitis / Q000175:diagnosis* / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D005706:Gallbladder Neoplasms / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques; D011401:Promoter Regions, Genetic; D012372:ROC Curve; D017422:Sequence Analysis, DNA
keywords: gallbladder cancer; global and gene-specific DNA methylation; molecular biomarkers panel; tumor suppressor genes