title: Nanoparticle/Polymer assembled microcapsules with pH sensing property.
abstract: The dual-labeled microcapsules via nanoparticle/polymer assembly based on polyamine-salt aggregates can be fabricated for the ratiometric intracellular pH sensing. After deposition of SiO2 nanoparticles on the poly(allylamine hydrochloride)/multivalent anionic salt aggregates followed by silicic acid treatment, the generated microcapsules are stable in a wide pH range (3.0 ∼ 8.0). pH sensitive dye and pH insensitive dye are simultaneously labeled on the capsules, which enable the ratiometric pH sensing. Due to the rough and positively charged surface, the microcapsules can be internalized by several kinds of cells naturally. Real-time measurement of intracellular pH in several living cells shows that the capsules are all located in acidic organelles after being taken up. Furthermore, the negatively charged DNA and dyes can be easily encapsulated into the capsules via charge interaction. The microcapsules with combination of localized pH sensing and drug loading abilities have many advantages, such as following the real-time transportation and processing of the carriers in cells.
authors: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
authors: Pan
authors: P
authors: Zhang
authors: Xiaoxue
authors: X
authors: Song
authors: Weijun
authors: W
authors: Tong
authors: Changyou
authors: C
authors: Gao
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D004339:Drug Compounding; D005456:Fluorescent Dyes; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011073:Polyamines / Q000737:chemistry*; D012824:Silicic Acid / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D055672:Static Electricity
chemical_list: D004337:Drug Carriers; D005456:Fluorescent Dyes; D011073:Polyamines; D012824:Silicic Acid; C063994:polyallylamine; D012822:Silicon Dioxide; D004247:DNA
keywords: cellular uptake; loading; microcapsules; nanoparticle/polymer assembly; pH sensing