title: A novel strategy for synthesis of hollow gold nanosphere and its application in electrogenerated chemiluminescence glucose biosensor.
abstract: Well-distributed hollow gold nanospheres (Aushell@GOD) (20 ± 5 nm) were synthesized using the glucose oxidase (GOD) cross-linked with glutaraldehyde as a template. A glucose biosensor was prepared based on Aushell@GOD nanospheres for catalyzing luminol electrogenerated chemiluminescence (ECL). Firstly, chitosan was modified in a glassy carbon electrode which offered an interface of abundant amino-groups to assemble Aushell@GOD nanospheres. Then, glucose oxidase was adsorbed on the surface of Aushell@GOD nanospheres via binding interactions between Aushell and amino groups of GOD to construct a glucose biosensor. The Aushell@GOD nanospheres were investigated with TEM and UV-vis. The ECL behaviors of the biosensor were also investigated. Results showed that, the obtained Aushell@GOD nanospheres exhibited excellent catalytic effect towards the ECL of luminol-H2O2 system. The response of the prepared biosensor to glucose was linear with the glucose concentration in the range of 1.0 μM to 4.3mM (R=0.9923) with a detection limit of 0.3 μM (signal to noise=3). This ECL biosensor exhibited short response time and excellent stability for glucose. At the same time the prepared ECL biosensor showed good reproducibility, sensitivity and selectivity.
authors: Key Laboratory of Luminescence and Real-time Analysis Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Xia
authors: X
authors: Zhong
authors: Key Laboratory of Luminescence and Real-time Analysis Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: yqchai@swu.edu.cn.
authors: Ya-Qin
authors: YQ
authors: Chai
authors: Key Laboratory of Luminescence and Real-time Analysis Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: yuanruo@swu.edu.cn.
authors: Ruo
authors: R
authors: Yuan
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D054118:Nanospheres / Q000737:chemistry / Q000648:ultrastructure; D015203:Reproducibility of Results; D013053:Spectrophotometry
chemical_list: D004800:Enzymes, Immobilized; D008165:Luminol; D006046:Gold; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose
keywords: Electrogenerated chemiluminescence; Glucose; Glucose oxidase; Glutaraldehyde; Nanospheres