title: Two alternative multiplex PCRs for the identification of the seven species of anglerfish (Lophius spp.) using an end-point or a melting curve analysis real-time protocol.
abstract: Anglerfish (Lophius spp.) is consumed worldwide and is an important economic resource though its seven species are often fraudulently interchanged due to their different commercial value, especially when sold in the form of fillets or pieces. Molecular analysis is the only possible mean to verify traceability and counteract fraud. We developed two multiplex PCRs, one end-point and one real-time with melting curve post-amplification analysis, which can even be run with the simplest two-channel thermocyclers. The two methods were tested on seventy-five reference samples. Their specificity was checked in twenty more species of those most commonly available on the market and in other species of the Lophiidae family. Both methods, the choice of which depends on the equipment and budget of the lab, provide a rapid and easy-to-read response, improving both the simplicity and cost-effectiveness of existing methods for identifying Lophius species.
authors: Department of Veterinary Sciences, University of Pisa, Via delle Piagge 2, 56124 Pisa, Italy. Electronic address: lorenzo.castigliego@unipi.it.
authors: Lorenzo
authors: L
authors: Castigliego
authors: Department of Veterinary Sciences, University of Pisa, Via delle Piagge 2, 56124 Pisa, Italy.
authors: Andrea
authors: A
authors: Armani
authors: Department of Veterinary Sciences, University of Pisa, Via delle Piagge 2, 56124 Pisa, Italy.
authors: Lara
authors: L
authors: Tinacci
authors: Department of Veterinary Sciences, University of Pisa, Via delle Piagge 2, 56124 Pisa, Italy.
authors: Daniela
authors: D
authors: Gianfaldoni
authors: Department of Veterinary Sciences, University of Pisa, Via delle Piagge 2, 56124 Pisa, Italy.
authors: Alessandra
authors: A
authors: Guidi
mesh_terms: D000818:Animals; D005399:Fishes; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
keywords: Lophius; Melting curve analysis; Multiplex PCR; Real-time PCR; Seafood frauds; Species-identification