title: Preventive effects of hydroalcoholic extract of saffron on hematological parameters of experimental asthmatic rats.
abstract: OBJECTIVE
  Asthma is a chronic inflammatory disease of the respiratory airways distinguished by edema and infiltration of inflammatory immune cells. To test our hypothesis about the anti-inflammatory effect of saffron, we examined effects of Crocus sativus (C. sativus) extract as a prophylactic anti-inflammatory agent in sensitized rats.
  
  
  METHODS
  To induce experimental asthma, rats were sensitized with injection and inhalation of ovalbumin (OA). Forty male Wistar rats were divided into 5 groups (n=8 for each): control, sensitized (asthma), and sensitized and pretreated with three different concentrations of extract, 50, 100, and 200 mg/kg, 2 times a week (group asthma+50EX, group asthma+100EX, and group asthma+200EX). After 32 days, total white blood cells (WBC) counts, red blood cells (RBC), and platelet counts in blood were examined.
  
  
  RESULTS
  Total WBC number and eosinophil and neutrophil percentage in blood were increased, but lymphocyte decreased in sensitized animals compared with those of control group (p<0.05 to p<0.001). We observed also elevated levels in RBC and platelet counts after sensitization in the asthma group. Pretreatment of sensitized rats in all concentrations decreased WBC count which was significant in first two concentrations (p<0.01 compared with group asthma). All concentrations of extract decreased eosinophil percentage significantly (p<0.001 compared with group asthma), however, for neutrophil percentage this improvement was not significant. Lymphocyte percentage increased in group asthma+100EX compared with group asthma (p<0.05). Moreover, in all concentrations, the extract reduced RBC and platelet count in pretreated sensitized rats compared with group asthma (p<0.01 to p<0.001).
  
  
  CONCLUSIONS
  Our findings indicated that the extract of C. sativus could be useful to prevent asthma as an anti-inflammatory treatment.
authors: Department of Biology, Payame Noor University, Boshruyeh, South Khorasan , I. R. Iran.
authors: Somayyeh
authors: S
authors: Vosooghi
authors: Applied Physiology Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad , I. R. Iran.
authors: Maryam
authors: M
authors: Mahmoudabady
authors: Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, I. R. Iran.
authors: Ali
authors: A
authors: Neamati
authors: Department of Biology, Faculty of Science, Arsanjan Branch, Islamic Azad University, I. R. Iran.
authors: Heydar
authors: H
authors: Aghababa
keywords: Asthma; Crocus sativus; Inflammation; Rat; Sensitization; WBC