title: Clinical and quality evaluation of red blood cell units collected via apheresis versus those obtained manually.
abstract: To evaluate the impact of collection procedure on the in vitro quality of red blood cells (RBC), we studied 30 units of apheresis-prepared RBC (ARBC) and 30 units of manually collected RBC (MRBC). We performed assays on day 1 and day 21 of the study, evaluating red cell mass volume (RCM); rate of hemolysis; pH, and levels of sodium, potassium, adenosine triphosphate (ATP), 2,3-diphosphoglycerate (2,3-DPG) and glucose. Eight patients with aplastic anemia received RBC transfusions of both components and their post-transfusion hematocrit (HCT) levels were compared. On day 21, we observed a significant drop of sodium and glucose levels in the ARBC group, compared with the MRBC group (P <.05). ARBC group demonstrated higher RCM that provided significantly higher HCT values to our group of anemic patients (P <.05). Hemolysis was significantly lower in the ARBC group, compared with the MRBC group (P <.05). At day 21, both groups had no detectable 2,3-DPG. Specimens from both groups retained ATP in sufficiently healthy amounts. The ARBC group demonstrated higher RCM and lower hemolysis levels compared with the MRBC group.
authors: Cairo University Blood Bank, Department of Clinical Pathology, Cairo University, Egypt; eimanhussein@ymail.com.
authors: Eiman
authors: E
authors: Hussein
authors: Hematology laboratory, Department of Clinical Pathology, Cairo University, Egypt.
authors: Azza
authors: A
authors: Enein
mesh_terms: D001781:Blood Component Removal; D001782:Blood Donors; D004912:Erythrocytes / Q000166:cytology*; D006403:Hematologic Tests; D006801:Humans
keywords: apheresis-prepared red cells; hemolysis; in vitro assay; in vivo assay; manually prepared red cells; storage days