title: Effect of Glucagon-like Peptide 2 on Tight Junction in Jejunal Epithelium of Weaned Pigs though MAPK Signaling Pathway.
abstract: The glucagon-like peptide 2 (GLP-2) that is expressed in intestine epithelial cells of mammals, is important for intestinal barrier function and regulation of tight junction (TJ) proteins. However, there is little known about the intracellular mechanisms of GLP-2 in the regulation of TJ proteins in piglets' intestinal epithelial cells. The purpose of this study is to test the hypothesis that GLP-2 regulates the expressions of TJ proteins in the mitogen-activated protein kinase (MAPK) signaling pathway in piglets' intestinal epithelial cells. The jejunal tissues were cultured in a Dulbecco's modified Eagle's medium/high glucose medium containing supplemental 0 to 100 nmol/L GLP-2. At 72 h after the treatment with the appropriate concentrations of GLP-2, the mRNA and protein expressions of zonula occludens-1 (ZO-1), occludin and claudin-1 were increased (p<0.05). U0126, an MAPK kinase inhibitor, prevented the mRNA and protein expressions of ZO-1, occludin, claudin-1 increase induced by GLP-2 (p<0.05). In conclusion, these results indicated that GLP-2 could improve the expression of TJ proteins in weaned pigs' jejunal epithelium, and the underlying mechanism may due to the MAPK signaling pathway.
authors: Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China ; Key Laboratory of Animal Disease-Resistance Nutrition and Feed Science, Ministry of Agriculture, Ya'an, Sichuan, 625014, China.
authors: Changsong
authors: C
authors: Yu
authors: Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China ; Key Laboratory of Animal Disease-Resistance Nutrition and Feed Science, Ministry of Agriculture, Ya'an, Sichuan, 625014, China.
authors: Gang
authors: G
authors: Jia
authors: Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China ; Key Laboratory of Animal Disease-Resistance Nutrition and Feed Science, Ministry of Agriculture, Ya'an, Sichuan, 625014, China.
authors: Yi
authors: Y
authors: Jiang
authors: Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China ; Key Laboratory of Animal Disease-Resistance Nutrition and Feed Science, Ministry of Agriculture, Ya'an, Sichuan, 625014, China.
authors: Qiuhong
authors: Q
authors: Deng
authors: College of Animal Medicine, Sichuan Agriculture University, Ya'an, Sichuan, 625014, China .
authors: Zhengli
authors: Z
authors: Chen
authors: College of Animal Medicine, Sichuan Agriculture University, Ya'an, Sichuan, 625014, China .
authors: Zhiwen
authors: Z
authors: Xu
authors: Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China ; Key Laboratory of Animal Disease-Resistance Nutrition and Feed Science, Ministry of Agriculture, Ya'an, Sichuan, 625014, China.
authors: Xiaolin
authors: X
authors: Chen
authors: Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China ; Key Laboratory of Animal Disease-Resistance Nutrition and Feed Science, Ministry of Agriculture, Ya'an, Sichuan, 625014, China.
authors: Kangning
authors: K
authors: Wang
keywords: Glucagon-like Peptide 2; Jejunal Epithelium; Mitogen-activated Protein Kinase Signaling Pathway; Tight Junction; Weaned Pig