title: Influence of self-assembling peptide nanofibre scaffolds on retinal differentiation potential of stem/progenitor cells derived from ciliary pigment epithelial cells.
abstract: Aim of the study was to investigate the influence of the self-assembling peptide nanofibre scaffolds (SAPNs) on the growth, proliferation and retinal neuronal differentiation of the stem/progenitor cells (SCs) derived from the ciliary pigment epithelium (CPE) of human cadaveric eye. Here SAPNs (RADA16-I, PM), which is well described in previous studies, commercially available and xeno-free. The CPE cells isolated were cultured in DMEM/F12 supplemented with N2 and growth factors such as basic fibroblast growth factor and epidermal growth factor, encapsulated in the scaffolds. The entrapped SCs actively expanded and formed clone-like clusters in the scaffolds. Many cells in the cluster were proliferating, as revealed by 5-bromo-2-deoxyuridine uptake and could be maintained for up to 6 days and expressed neural progenitor markers such as β-III tubulin, Nestin, Pax6 and Musashi1. Upon differentiation of these cells in conditioned medium, the cells exhibited retinal neuronal markers such as s-Opsin, rhodopsin and Recoverin. The RT2profiler polymerase chain reaction array experiments showed selective gene expression, possibly involved in neural stem/progenitor cell adhesion and differentiation. These findings suggest the suitability of the three-dimensional culture system for the proliferation and maintenance of CPE stem/progenitor cells (CPE-NS) and for possible use in ex vivo studies of small molecules, drug deliveries for retinal diseases and for use in combination with directed stem/progenitor cell differentiation. and ultimately for tissue replacement therapies. Copyright © 2014 John Wiley & Sons, Ltd.
authors: L&T Department of Ocular Pathology.|Radheshyam Kanoi Stem Cell Laboratory, Vision Research Foundation, Sankara Nethralaya, Tamilnadu, India.
authors: Srilatha
authors: S
authors: Jasty
authors: L&T Department of Ocular Pathology.
authors: Saranya
authors: S
authors: Suriyanarayanan
authors: L&T Department of Ocular Pathology.
authors: Subramanian
authors: S
authors: Krishnakumar
mesh_terms: D002102:Cadaver; D002448:Cell Adhesion; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D002924:Ciliary Body / Q000166:cytology; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000166:cytology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology*; D010455:Peptides / Q000737:chemistry*; D055351:Retinal Neurons / Q000378:metabolism; D055213:Retinal Pigment Epithelium / Q000166:cytology*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D017077:Culture Media, Conditioned; D010455:Peptides; C546681:RADA16-I; D016222:Fibroblast Growth Factor 2
keywords: adult stem cells; ciliary pigment epithelial cells; retinal cells; self assembling peptide nanofibre scaffolds; stem cell biology; tissue engineering