title: Comparison of the rhizosphere bacterial communities of Zigongdongdou soybean and a high-methionine transgenic line of this cultivar.
abstract: Previous studies have shown that methionine from root exudates affects the rhizosphere bacterial population involved in soil nitrogen fixation. A transgenic line of Zigongdongdou soybean cultivar (ZD91) that expresses Arabidopsis cystathionine γ-synthase resulting in an increased methionine production was examined for its influence to the rhizosphere bacterial population. Using 16S rRNA gene-based pyrosequencing analysis of the V4 region and DNA extracted from bacterial consortia collected from the rhizosphere of soybean plants grown in an agricultural field at the pod-setting stage, we characterized the populational structure of the bacterial community involved. In total, 87,267 sequences (approximately 10,908 per sample) were analyzed. We found that Acidobacteria, Proteobacteria, Bacteroidetes, Actinobacteria, Chloroflexi, Planctomycetes, Gemmatimonadetes, Firmicutes, and Verrucomicrobia constitute the dominant taxonomic groups in either the ZD91 transgenic line or parental cultivar ZD, and that there was no statistically significant difference in the rhizosphere bacterial community structure between the two cultivars.
authors: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
authors: Jingang
authors: J
authors: Liang
authors: The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, The Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Shi
authors: S
authors: Sun
authors: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
authors: Jun
authors: J
authors: Ji
authors: Nanchong Academy of Agricultural Science, Nanchong, China.
authors: Haiying
authors: H
authors: Wu
authors: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
authors: Fang
authors: F
authors: Meng
authors: Nanchong Academy of Agricultural Science, Nanchong, China.
authors: Mingrong
authors: M
authors: Zhang
authors: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
authors: Xiaobo
authors: X
authors: Zheng
authors: The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), MOA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Science, The Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Cunxiang
authors: C
authors: Wu
authors: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.
authors: Zhengguang
authors: Z
authors: Zhang
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D008715:Methionine / Q000378:metabolism*; D009586:Nitrogen Fixation; D030821:Plants, Genetically Modified / Q000378:metabolism / Q000382:microbiology*; D013025:Glycine max / Q000378:metabolism / Q000382:microbiology*
chemical_list: D008715:Methionine