title: Antifungal activity and inhibition of fumonisin production by Rosmarinus officinalis L. essential oil in Fusarium verticillioides (Sacc.) Nirenberg.
abstract: The chemical composition of Rosmarinus officinalis L. essential oil (REO) was analysed by gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy. The main compounds of the REO were 1.8 cineole (52.2%), camphor (15.2%) and α-pinene (12.4%). The mycelial growth of Fusarium verticillioides (Sacc.) Nirenberg was reduced significantly by 150 μg/mL of REO. Significant microscopic morphological changes were visualised, such as the rupture of the cell wall and the leakage of cytoplasm at 300 μg/mL of REO. At lower concentrations of REO, the effects on the production of ergosterol and the biomass of mycelium varied, as did the effects on the production of fumonisins, but at ≥300 μg/mL of REO, these processes were significantly inhibited, showing the effectiveness of the REO as an antifungal agent. The results suggested that the REO acts against F. verticillioides by disrupting the cell wall and causing the loss of cellular components, subsequently inhibiting the production of fumonisins and ergosterol.
authors: Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, Brazil.
authors: Natalia
authors: N
authors: da Silva Bomfim
authors: Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, Brazil.
authors: Lydiana Polis
authors: LP
authors: Nakassugi
authors: Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, Brazil.
authors: Jessica
authors: J
authors: Faggion Pinheiro Oliveira
authors: Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, Brazil.
authors: Cassia Yumie
authors: CY
authors: Kohiyama
authors: Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, Brazil.
authors: Simone Aparecida Galerani
authors: SAG
authors: Mossini
authors: Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, Brazil.
authors: Renata
authors: R
authors: Grespan
authors: Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, Brazil.
authors: Samuel Botião
authors: SB
authors: Nerilo
authors: Laboratory of Mycotoxicological Analysis, Federal University of Santa Maria, Santa Maria 97105-900, Brazil.
authors: Carlos Augusto
authors: CA
authors: Mallmann
authors: Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, Brazil.
authors: Benicio
authors: B
authors: Alves Abreu Filho
authors: Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, Brazil. Electronic address: mmjunior@uem.br.
authors: Miguel
authors: M
authors: Machinski
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry*; D037341:Fumonisins / Q000032:analysis*; D005670:Fusarium / Q000187:drug effects*; D009822:Oils, Volatile / Q000737:chemistry*; D027542:Rosmarinus / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D037341:Fumonisins; D009822:Oils, Volatile
keywords: Antifungal; Ergosterol; Fumonisins; Fusarium verticillioides; Rosemary essential oil