title: Corecovery of lipids and fermentable sugars from Rhodosporidium toruloides using ionic liquid cosolvents: application of recycle to batch fermentation.
abstract: This work evaluates the ability of an ionic liquid-methanol cosolvent system to extract lipids and recycle fermentable sugars recovered from oil-bearing Rhodosporidium toruloides grown in batch culture on defined media using glucose and xylose as carbon sources. Growth on the recycled mixed carbon substrate was successful with glucose consumed before xylose and overall cell mass to lipid yields (YP/X ) between 57% and 61% (w/w relative to whole dried cell mass) achieved. Enzymatic hydrolysis of the delipified carbohydrate fraction recovered approximately 9%-11% (w/w) of the whole dried cell mass as fermentable sugars, which were successfully recycled as carbon sources without further purification. In total, up to 70% (w/w) of the whole dried cell mass was recovered as lipids and fermentable sugars and the substrate to lipid yields (YP/S ) was increased from 0.12 to 0.16 g lipid/g carbohydrate consumed, highlighting the promise of this approach to process lipid bearing cell biomass.
authors: University of Hawaii at Manoa, Hawaii Natural Energy Inst., 1680 East West Road, POST 109, Honolulu, Hawaii, 96822.
authors: Godwin
authors: G
authors: Severa
authors: Guneet
authors: G
authors: Kumar
authors: Michael J
authors: MJ
authors: Cooney
mesh_terms: D018533:Biomass; D019149:Bioreactors; D002241:Carbohydrates / Q000737:chemistry / Q000302:isolation & purification*; D005285:Fermentation; D052578:Ionic Liquids; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification*; D059027:Recycling / Q000379:methods*; D014587:Ustilaginales / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D052578:Ionic Liquids; D008055:Lipids
keywords: Rhodosporidium toruloides; cosolvent extraction; enzyme saccharization; fermentable sugars; ionic liquids; lipids