title: Gene chip/PCR-array analysis of tissue response to 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer surfaces in a mouse subcutaneous transplantation system.
abstract: To evaluate the in vivo foreign body reaction to bio-inert 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers, MPC polymer-coated porous substrates, with large surface area, were implanted subcutaneously in mice for 7 and 28 days, and the surrounding tissue response and cells infiltrating into the porous structure were evaluated. The MPC polymer surface induced low angiogenesis and thin encapsulation around the porous substrate, and slightly suppressed cell infiltration into the porous substrate. M1-type macrophage specific gene (CCR7) expression was suppressed by the MPC polymer surface after 7 days, resulting in the suppression of inflammatory cytokine/chemokine gene expression. However, the expression of these genes on the MPC polymer surface was higher than on the non-coated surface after 28 days. These findings suggest that MPC polymer surfaces successfully inhibit inflammatory responses during the early stage of tissue response, and seem to retard its occurrence over time.
authors: a Department of Biomedical Engineering , National Cerebral and Cardiovascular Center Research Institute , 5-7-1 Fujishiro-dai, Suita , Osaka 565-8565 , Japan.
authors: Sachiro
authors: S
authors: Kakinoki
authors: Yusuke
authors: Y
authors: Sakai
authors: Taro
authors: T
authors: Takemura
authors: Nobutaka
authors: N
authors: Hanagata
authors: Toshia
authors: T
authors: Fujisato
authors: Kazuhiko
authors: K
authors: Ishihara
authors: Tetsuji
authors: T
authors: Yamaoka
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry; D018925:Chemokines / Q000235:genetics; D005355:Fibrosis; D005549:Foreign-Body Reaction / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D020959:Polyethylene / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011108:Polymers / Q000009:adverse effects* / Q000737:chemistry*; D016062:Porosity; D019736:Prostheses and Implants / Q000009:adverse effects*; D012867:Skin; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D018925:Chemokines; D008689:Methacrylates; D011108:Polymers; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; D020959:Polyethylene
keywords: bio-inert; foreign body reaction; inflammation; poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate); porous substrate