title: The chemokine CCL5 induces selective migration of bovine classical monocytes and drives their differentiation into LPS-hyporesponsive macrophages in vitro.
abstract: Human and mouse studies indicate distinct roles of selected chemokines for monocyte subset attraction. We therefore analyzed the still unknown sensitivity and response of bovine monocyte subsets toward two monocyte-attracting chemokines (CCL2, CCL5). Only CCL5 induced a significant Ca(2+)influx and migration response in bovine monocytes, with classical and intermediate monocytes being significantly stimulated and attracted compared to nonclassical monocytes. The presence of CCL5 during in vitro macrophage differentiation did not alter their capacity to phagocytize or to generate reactive oxygen species upon stimulation with E. coli. However, macrophages differentiated in the presence of CCL5 displayed an altered phenotype with significantly less expressed CD14 and MHC class II molecules, whereas CD16 was upregulated. Moreover, CCL5-differentiated macrophages displayed a reduced upregulation of CXCL8, ARG1, IL6 and IL10 mRNA. Taken together, CCL5 but not CCL2 mainly attract bovine classical monocytes and promote their differentiation into LPS-hypo-responsive macrophages.
authors: Immunology Unit, University of Veterinary Medicine Foundation, Bischofsholer Damm 15, D-30173 Hannover, Germany.
authors: Jamal
authors: J
authors: Hussen
authors: Immunology Unit, University of Veterinary Medicine Foundation, Bischofsholer Damm 15, D-30173 Hannover, Germany.
authors: Constanze
authors: C
authors: Frank
authors: Immunology Unit, University of Veterinary Medicine Foundation, Bischofsholer Damm 15, D-30173 Hannover, Germany; HIPRA Deutschland GmbH, Feldstraße 21, D-40479 Düsseldorf, Germany.
authors: Anna
authors: A
authors: Düvel
authors: Immunology Unit, University of Veterinary Medicine Foundation, Bischofsholer Damm 15, D-30173 Hannover, Germany.
authors: Mirja
authors: M
authors: Koy
authors: Immunology Unit, University of Veterinary Medicine Foundation, Bischofsholer Damm 15, D-30173 Hannover, Germany. Electronic address: hans-joachim.schuberth@tiho-hannover.de.
authors: Hans-Joachim
authors: HJ
authors: Schuberth
mesh_terms: D000818:Animals; D001119:Arginase / Q000235:genetics / Q000276:immunology; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D018932:Chemokine CCL2 / Q000494:pharmacology; D018946:Chemokine CCL5 / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects*; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology; D017136:Ion Transport / Q000187:drug effects; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D061251:Primary Cell Culture; D017382:Reactive Oxygen Species / Q000378:metabolism; D017452:Receptors, IgG / Q000235:genetics / Q000276:immunology
chemical_list: D018932:Chemokine CCL2; D018946:Chemokine CCL5; D015850:Interleukin-6; D016209:Interleukin-8; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D017452:Receptors, IgG; D016753:Interleukin-10; D001119:Arginase; D002118:Calcium
keywords: Cattle; Chemokine; Macrophage; Monocyte