title: G-quadruplex DNA biosensor for sensitive visible detection of genetically modified food.
abstract: In this paper, a novel label-free G-quadruplex DNAzyme sensor has been proposed for colorimetric identification of GMO using CaMV 35S promoter sequence as the target. The binary probes can fold into G-quadruplex structure in the presence of DNA-T (Target DNA) and then combine with hemin to form a DNAzyme resembling horseradish peroxidase. The detection system consists of two G-rich probes with 2:2 split mode by using the absorbance and color of ABTS(2-) as signal reporter. Upon the addition of a target sequence, two probes both hybridize with target and then their G-rich sequences combine to form a G-quadruplex DNAzyme, and the DNAzyme can catalyze the reaction of ABTS(2-) with H2O2. Then the linear range is from 0.05 to 0.5 μM while detection limit is 5nM. These results demonstrate that the proposed G-quadruplex DNAzyme method could be used as a simple, sensitive and cost-effective approach for assays of GMO.
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China; School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen, Guangdong 518055, China.
authors: Xiaohua
authors: X
authors: Jiang
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China.
authors: Huimin
authors: H
authors: Zhang
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China.
authors: Jun
authors: J
authors: Wu
authors: Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
authors: Xiang
authors: X
authors: Yang
authors: Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China. Electronic address: shaojingwei@fzu.edu.cn.
authors: Jingwei
authors: J
authors: Shao
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China.
authors: Yujing
authors: Y
authors: Lu
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China. Electronic address: summer328cn@163.com.
authors: Bin
authors: B
authors: Qiu
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China.
authors: Zhenyu
authors: Z
authors: Lin
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China.
authors: Guonan
authors: G
authors: Chen
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D021881:DNA, Catalytic / Q000737:chemistry; D005504:Food Analysis / Q000379:methods; D030841:Food, Genetically Modified; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry; D006427:Hemin / Q000737:chemistry; D015203:Reproducibility of Results; D013025:Glycine max / Q000235:genetics; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D021881:DNA, Catalytic; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006147:Guanine; D006427:Hemin; D004247:DNA
keywords: Colorimetric; G-quadruplex; GMO