title: Crucial role of the Rcl1p-Bms1p interaction for yeast pre-ribosomal RNA processing.
abstract: The essential Rcl1p and Bms1p proteins form a complex required for 40S ribosomal subunit maturation. Bms1p is a GTPase and Rcl1p has been proposed to catalyse the endonucleolytic cleavage at site A2 separating the pre-40S and pre-60S maturation pathways. We determined the 2.0 Å crystal structure of Bms1p associated with Rcl1p. We demonstrate that Rcl1p nuclear import depends on Bms1p and that the two proteins are loaded into pre-ribosomes at a similar stage of the maturation pathway and remain present within pre-ribosomes after cleavage at A2. Importantly, GTP binding to Bms1p is not required for the import in the nucleus nor for the incorporation of Rcl1p into pre-ribosomes, but is essential for early pre-rRNA processing. We propose that GTP binding to Bms1p and/or GTP hydrolysis may induce conformational rearrangements within the Bms1p-Rcl1p complex allowing the interaction of Rcl1p with its RNA substrate.
authors: Institut Européen de Chimie et Biologie, ARNA laboratory, Université de Bordeaux, F-33607 Pessac, France Institut National de la Santé Et de la Recherche Médicale, INSERM - U869, ARNA laboratory, F-33000 Bordeaux, France.
authors: Anna
authors: A
authors: Delprato
authors: Equipe labellisée Ligue Contre le Cancer, Centre National de la Recherche Scientifique, Laboratoire de Biologie Moléculaire Eucaryote and Université de Toulouse, UPS, F-31062 Toulouse Cedex 9, France.
authors: Yasmine
authors: Y
authors: Al Kadri
authors: Institut Européen de Chimie et Biologie, ARNA laboratory, Université de Bordeaux, F-33607 Pessac, France Institut National de la Santé Et de la Recherche Médicale, INSERM - U869, ARNA laboratory, F-33000 Bordeaux, France.
authors: Natacha
authors: N
authors: Pérébaskine
authors: Institut Européen de Chimie et Biologie, ARNA laboratory, Université de Bordeaux, F-33607 Pessac, France Institut National de la Santé Et de la Recherche Médicale, INSERM - U869, ARNA laboratory, F-33000 Bordeaux, France.
authors: Cécile
authors: C
authors: Monfoulet
authors: Equipe labellisée Ligue Contre le Cancer, Centre National de la Recherche Scientifique, Laboratoire de Biologie Moléculaire Eucaryote and Université de Toulouse, UPS, F-31062 Toulouse Cedex 9, France.
authors: Yves
authors: Y
authors: Henry
authors: Equipe labellisée Ligue Contre le Cancer, Centre National de la Recherche Scientifique, Laboratoire de Biologie Moléculaire Eucaryote and Université de Toulouse, UPS, F-31062 Toulouse Cedex 9, France anthony.henras@ibcg.biotoul.fr.
authors: Anthony K
authors: AK
authors: Henras
authors: Institut Européen de Chimie et Biologie, ARNA laboratory, Université de Bordeaux, F-33607 Pessac, France Institut National de la Santé Et de la Recherche Médicale, INSERM - U869, ARNA laboratory, F-33000 Bordeaux, France sebastien.fribourg@inserm.fr.
authors: Sébastien
authors: http://orcid.org/0000-0003-4112-7510
authors: S
authors: Fribourg
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006160:Guanosine Triphosphate / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017354:Point Mutation; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: C436684:Bms1 protein, S cerevisiae; D009687:Nuclear Proteins; C409342:RCL1 protein, S cerevisiae; D012322:RNA Precursors; D012335:RNA, Ribosomal; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins