title: miR-144 suppresses the proliferation and metastasis of hepatocellular carcinoma by targeting E2F3.
abstract: MicroRNAs (miRNAs) play essential roles in the progression of hepatocellular carcinoma (HCC). miR-144 acts as a tumor suppressor in some malignancies, while its role in HCC is unclear. Here, we found that miR-144 was significantly decreased in HCC tissues and cell lines. Forced overexpression of miR-144 remarkably reduced cell proliferation, increased apoptosis, and suppressed migration and invasion of HCC cells. E2F transcription factor 3 (E2F3) was identified as a target of miR-144 in HCC cells. Moreover, E2F3 overexpression partially attenuated the tumor suppressive effects of miR-144, and the expression of E2F3 was negatively correlated with miR-144 level in HCC tissues. Our data suggest that miR-144 might suppress the growth and motility of HCC cells partially by targeting E2F3.
authors: Department of General Surgery, Wuhan General Hospital of Guangzhou Command, 627 Wuluo Road, Wuhan, 430070, China.
authors: Tingjia
authors: T
authors: Cao
authors: Hanjun
authors: H
authors: Li
authors: Yilin
authors: Y
authors: Hu
authors: Dandan
authors: D
authors: Ma
authors: Xun
authors: X
authors: Cai
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D002465:Cell Movement; D049109:Cell Proliferation; D050699:E2F3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D050699:E2F3 Transcription Factor; C098319:E2F3 protein, human; C554293:MIRN144 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger