title: Assay to probe proteolytic processing of Notch by γ-secretase.
abstract: With the increasing appreciation of the role of Notch in development and disease, measuring its cleavage and signaling activity in cellular systems has become important. Here we describe a cell-based method to analyze the cleavage of Notch at the S3 site by γ-secretase. HEK cells are transfected with an N-terminal truncated and myc-labeled mNotchΔE construct which can be easily and quantitatively detected by western blotting.
authors: VIB Center for the Biology of Disease - VIB11 and Center for Human Genetics, VIB and KU Leuven, O&N 4, Herestraat 49, 602, Leuven, 3000, Belgium.
authors: Lutgarde
authors: L
authors: Serneels
authors: Ina
authors: I
authors: Tesseur
authors: Bart
authors: B
authors: De Strooper
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D059748:Proteolysis; D051880:Receptors, Notch / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014162:Transfection / Q000379:methods
chemical_list: D051880:Receptors, Notch; D053829:Amyloid Precursor Protein Secretases