title: Sox4 up-regulates Cyr61 expression in colon cancer cells.
abstract: OBJECTIVE
  Genetic changes leading to aberrant activation of oncogenes are viewed as a crucial step in colon cancer. Sox4, a member of Sox (Sry-box) family of transcription factors, plays a critical role in tumorigenesis.
  
  
  METHODS
  PCR-based microarrays were used to identify potential transcriptional target of Sox4. siRNA was used to knockdown the expression of Sox4. Luciferase and chromatin immunoprecipitation (ChIP) assays were used to test the transcriptional regulations.
  
  
  RESULTS
  PCR-based microarrays found that Cyr61, a secreted extracellular matrix-associated signaling protein, was a transcriptional target of Sox4. Overexpression of Sox4 increased, while its knockdown using small interfering RNA (siRNA) reduced Cyr61 expression. A potential Sox4 binding motif located at the proximal Cyr61 promoter was identified.
  
  
  CONCLUSIONS
  Thus, our results suggest a previously unknown Sox4-Cyr61 molecular network, which may control colon cancer cell proliferation and survival.
authors: Department of General Surgery, Henan Provincial People' s Hospital, People's Hospital of Zhengzhou University, Zhengzhou, People' s Republic of China.
authors: Gang
authors: G
authors: Wu
authors: Yuan-Zeng
authors: YZ
authors: Zhu
authors: Jian-Cheng
authors: JC
authors: Zhang
mesh_terms: D001483:Base Sequence; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D055515:Cysteine-Rich Protein 61 / Q000378:metabolism*; D017931:DNA Primers; D006801:Humans; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D055751:SOXC Transcription Factors / Q000502:physiology*; D015398:Signal Transduction; D015854:Up-Regulation / Q000502:physiology*
chemical_list: C501848:CCN1 protein, human; D055515:Cysteine-Rich Protein 61; D017931:DNA Primers; C083431:SOX4 protein, human; D055751:SOXC Transcription Factors