title: The distribution of transplanted human mesenchymal stem cells in the CNS of young Macaca fascicularis.
abstract: Mesenchymal stem cell (MSC)-based therapies have generated much hope and promise as a potential source of cells for cell-based therapeutic strategies in pediatric degenerative diseases. However, the distribution and migratory routes of MSCs are unknown. Here, real-time PCR and microscopy were used to observe the migration and distribution of labeled human MSCs (hMSCs) transplanted into the striatum of young Macaca fascicularis. Moreover, the differentiation of hMSCs was also detected using immunofluorescence. We found that hMSCs were mainly located near the injection site in the brain and in the anterior brain after 2 weeks. After 4 weeks, the hMSCs had dispersed and could be detected in each brain slice and were more uniformly distributed than after 2 weeks. The hMSCs showed a preference for migration towards blood vessels, which may be one of the migratory routes used by hMSCs. Additionally, hMSCs could be observed to give rise to NeuN- and GFAP-positive cells. Transplanted hMSCs also increased the expression levels of N-cadherin in the host brain tissue, which may be one factor that drives the migration and differentiation of hMSCs after transplantation. These results provide preclinical evidence that MSC-based therapies may represent an efficacious alternative to more conventional treatment regimens for a variety of pediatric neurologic disorders.
authors: Comparative Medical Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Science (CAMS), Beijing 100005, China; Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors: Jiamei
authors: J
authors: Li
authors: Comparative Medical Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Science (CAMS), Beijing 100005, China.
authors: Hua
authors: H
authors: Zhu
authors: Comparative Medical Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Science (CAMS), Beijing 100005, China.
authors: Yunxin
authors: Y
authors: Chen
authors: Comparative Medical Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Science (CAMS), Beijing 100005, China.
authors: Wei
authors: W
authors: Deng
authors: Motac Collaborative Laboratory, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Science (CAMS), Beijing 100005, China.
authors: Qin
authors: Q
authors: Li
authors: Institute of Basic Medical Sciences and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.
authors: Shan
authors: S
authors: Lu
authors: Comparative Medical Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Science (CAMS), Beijing 100005, China.
authors: Yanfeng
authors: Y
authors: Xu
authors: Comparative Medical Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Science (CAMS), Beijing 100005, China.
authors: Lan
authors: L
authors: Huang
authors: Comparative Medical Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Science (CAMS), Beijing 100005, China.
authors: Chunmei
authors: C
authors: Ma
authors: Institute of Basic Medical Sciences and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.
authors: Chunhua
authors: C
authors: Zhao
authors: Peking Union Medical College Hospital, Beijing 100730, China.
authors: Renzhi
authors: R
authors: Wang
authors: Comparative Medical Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Science (CAMS), Beijing 100005, China. Electronic address: qinchuan@pumc.edu.cn.
authors: Chuan
authors: C
authors: Qin
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D015820:Cadherins / Q000378:metabolism; D002421:Caudate Nucleus / Q000166:cytology; D002454:Cell Differentiation; D002465:Cell Movement; D005260:Female; D006801:Humans; D008252:Macaca fascicularis; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*
chemical_list: D015820:Cadherins
keywords: Central nervous system; Differentiation; Macaca fascicularis; Mesenchymal stem cells; Migration; N-cadherin; Transplantation