title: Rapid colorimetric sensing of tetracycline antibiotics with in situ growth of gold nanoparticles.
abstract: A colorimetric assay utilizing the formation of gold nanoparticles was developed to detect tetracycline antibiotics in fluidic samples. Tetracycline antibiotics showed the capability of directly reducing aurate salts into atomic gold which form gold nanoparticles spontaneously under proper conditions. The resulted gold nanoparticles showed characteristic plasmon absorbance at 526 nm, which can be visualized by naked eyes or with a spectrophotometer. UV-vis absorbance of the resulted gold nanoparticles is correlated directly with the concentrations of tetracycline antibiotics in the solution, allowing for quantitative colorimetric detection of tetracycline antibiotics. Reaction conditions, such as pH, temperature, reaction time, and ionic strength were optimized. Sensitivity of the colorimetric assay can be enhanced by the addition of gold nanoparticle seeds, a LOD as low as 20 ng mL(-1) can be achieved with the help of seed particles. The colorimetric assay showed minimum interference from ethanol, methanol, urea, glucose, and other antibiotics such as sulfonamides, amino glycosides etc. Validity of the method was also evaluated on urine samples spiked with tetracycline antibiotics. The method provides a broad spectrum detection method for rapid and sensitive detection of reductive substances such as tetracycline antibiotics in liquid and biological samples.
authors: Logistics School, Beijing Wuzi University, Beijing 101149, China. Electronic address: lishen97@163.com.
authors: Li
authors: L
authors: Shen
authors: Logistics School, Beijing Wuzi University, Beijing 101149, China.
authors: Jing
authors: J
authors: Chen
authors: Logistics School, Beijing Wuzi University, Beijing 101149, China.
authors: Na
authors: N
authors: Li
authors: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100094, China.
authors: Pingli
authors: P
authors: He
authors: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors: Zhen
authors: Z
authors: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D013056:Spectrophotometry, Ultraviolet; D013754:Tetracyclines / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D013754:Tetracyclines; D006046:Gold
keywords: Colorimetric assay; Gold nanoparticles; Tetracycline antibiotics; Urine sample