title: Nanoelectrode for amperometric monitoring of individual vesicular exocytosis inside single synapses.
abstract: Chemical neurotransmission occurs at chemical synapses and endocrine glands, but up to now there was no means for direct monitoring of neurotransmitter exocytosis fluxes and their precise kinetics from inside an individual synapse. The fabrication of a novel finite conical nanoelectrode is reported perfectly suited in size and electrochemical properties for probing amperometrically inside what appears to be single synapses and monitoring individual vesicular exocytotic events in real time. This allowed obtaining direct and important physiological evidences which may yield important and new insights into the nature of synaptic communications.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China).
authors: Yu-Tao
authors: YT
authors: Li
authors: Shu-Hui
authors: SH
authors: Zhang
authors: Li
authors: L
authors: Wang
authors: Rong-Rong
authors: RR
authors: Xiao
authors: Wei
authors: W
authors: Liu
authors: Xin-Wei
authors: XW
authors: Zhang
authors: Zhuan
authors: Z
authors: Zhou
authors: Christian
authors: C
authors: Amatore
authors: Wei-Hua
authors: WH
authors: Huang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D005089:Exocytosis; D008839:Microelectrodes; D036103:Nanotechnology; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism; D017783:Superior Cervical Ganglion / Q000166:cytology; D013569:Synapses / Q000378:metabolism*
chemical_list: D018377:Neurotransmitter Agents
keywords: amperometry; complex exocytosis; nanoelectrodes; neurotransmitters; synapses