title: A melanocyte--melanoma precursor niche in sweat glands of volar skin.
abstract: Determination of the niche for early-stage cancer remains a challenging issue. Melanoma is an aggressive cancer of the melanocyte lineage. Early melanoma cells are often found in the epidermis around sweat ducts of human volar skin, and the skin pigmentation pattern is an early diagnostic sign of acral melanoma. However, the niche for melanoma precursors has not been determined yet. Here, we report that the secretory portion (SP) of eccrine sweat glands provide an anatomical niche for melanocyte-melanoma precursor cells. Using lineage-tagged H2B-GFP reporter mice, we found that melanoblasts that colonize sweat glands during development are maintained in an immature, slow-cycling state but renew themselves in response to genomic stress and provide their differentiating progeny to the epidermis. FISH analysis of human acral melanoma expanding in the epidermis revealed that unpigmented melanoblasts with significant cyclin D1 gene amplification reside deep in the SP of particular sweat gland(s). These findings indicate that sweat glands maintain melanocyte-melanoma precursors in an immature state in the niche and explain the preferential distribution of early melanoma cells around sweat glands in human volar skin.
authors: Department of Stem Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan; Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors: Natsuko
authors: N
authors: Okamoto
authors: Takahiro
authors: T
authors: Aoto
authors: Hisashi
authors: H
authors: Uhara
authors: Satoshi
authors: S
authors: Yamazaki
authors: Hidenori
authors: H
authors: Akutsu
authors: Akihiro
authors: A
authors: Umezawa
authors: Hiromitsu
authors: H
authors: Nakauchi
authors: Yoshiki
authors: Y
authors: Miyachi
authors: Toshiaki
authors: T
authors: Saida
authors: Emi K
authors: EK
authors: Nishimura
mesh_terms: D000818:Animals; D002453:Cell Cycle; D019938:Cyclin D1 / Q000235:genetics; D005784:Gene Amplification; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008544:Melanocytes / Q000473:pathology*; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D014411:Neoplastic Stem Cells / Q000473:pathology*; D012867:Skin / Q000473:pathology*; D012878:Skin Neoplasms / Q000473:pathology*; D055153:Stem Cell Niche; D013545:Sweat Glands / Q000473:pathology*
chemical_list: D019938:Cyclin D1; D049452:Green Fluorescent Proteins
keywords: melanocyte stem cell; melanoma; sweat gland/niche