title: Zoledronate and ion-releasing resins impair dentin collagen degradation.
abstract: This study analyzed the amounts of solubilized telopeptides cross-linked carboxyterminal telopeptide of type I collagen (ICTP) and C-terminal crosslinked telopeptide of type I collagen (CTX) derived from matrix-metalloproteinases (MMPs) and cysteine cathepsins (CTPs) subsequent to application of a filler-free (Res.A) or an ion-releasing resin (Res.B) to ethylenediaminetetraacetic acid (EDTA)-demineralized dentin with or without zoledronate-containing primer (Zol-primer) pre-treatment. The chemical modification induced following treatments and artificial saliva (AS) storage was also analyzed through attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). Totally EDTA-demineralized specimens were infiltrated with Res.A or Res.B with or without Zol-primer pre-treatment, light-cured, and immersed in AS for up to 4 wk. ICTP release was reduced following infiltration with Res.B and further reduced when Res.B was used with Zol-primer; remarkable phosphate mineral uptake was attained after AS storage. CTX release was increased in Res.A- and Res.B-treated dentin. However, when Zol-primer was used with Res.A, the CTX release fell significantly compared to the other tested resin-infiltration methods. In conclusion, zoledronate offers an additional inhibitory effect to the ion-releasing resins in MMP-mediated collagen degradation. However, Zol-primer induces a modest reduction in CTX release only when used with resin-based systems containing no ion-releasing fillers.
authors: Adhesive Dentistry Research Group, Institute of Dentistry, and TYKS University Hospital, University of Turku, Turku, Finland.
authors: A
authors: A
authors: Tezvergil-Mutluay
authors: Adhesive Dentistry Research Group, Institute of Dentistry, and TYKS University Hospital, University of Turku, Turku, Finland.
authors: R
authors: R
authors: Seseogullari-Dirihan
authors: Department of Restorative Dentistry, College of Dentistry, Federal University of Ceará, Fortaleza, Brazil.
authors: V P
authors: VP
authors: Feitosa
authors: Department of Endodontics, College of Dental Medicine, Georgia Health Sciences University, Augusta, GA, USA.
authors: F R
authors: FR
authors: Tay
authors: Biomaterials, Biomimetics & Biophotonics, King's College London Dental Institute at Guy's Hospital, London, UK.
authors: T F
authors: TF
authors: Watson
authors: Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA.
authors: D H
authors: DH
authors: Pashley
authors: Dental Biomaterials and Minimally Invasive Dentistry, Departamento de Odontología, Facultad de Ciencias de la Salud, CEU-Cardenal Herrera University, Valencia, Spain salvatore.sauro@uch.ceu.es.
authors: S
authors: S
authors: Sauro
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology; D002133:Calcium Sulfate / Q000494:pharmacology; D002403:Cathepsins / Q000037:antagonists & inhibitors / Q000494:pharmacology; D003094:Collagen / Q000187:drug effects*; D024042:Collagen Type I / Q000032:analysis; D003804:Dentin / Q000187:drug effects*; D004164:Diphosphonates / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology*; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology; D020782:Matrix Metalloproteinases / Q000494:pharmacology; D010455:Peptides / Q000032:analysis; D019279:Resin Cements / Q000494:pharmacology*; D012464:Saliva, Artificial / Q000737:chemistry; D017640:Silicates / Q000494:pharmacology; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D017001:Tooth Demineralization / Q000139:chemically induced; D000077211:Zoledronic Acid
chemical_list: D017607:Aluminum Compounds; D050071:Bone Density Conservation Agents; D017610:Calcium Compounds; D002130:Calcium Phosphates; D024042:Collagen Type I; D004164:Diphosphonates; D007093:Imidazoles; D061965:Matrix Metalloproteinase Inhibitors; D010455:Peptides; D019279:Resin Cements; D012464:Saliva, Artificial; D017640:Silicates; C485817:beta-tricalcium phosphate; C052929:collagen type I trimeric cross-linked peptide; C013481:dicalcium silicate; C035219:calcium aluminate; C506393:tricalcium silicate; D000077211:Zoledronic Acid; D003094:Collagen; D004492:Edetic Acid; D002403:Cathepsins; D020782:Matrix Metalloproteinases; D002133:Calcium Sulfate
keywords: bisphosphonates; calcium phosphates; cathepsins; matrix metalloproteinase; phosphorylation; proteolysis