title: [Effects of surface roughness of bone cements on histological characteristics of induced membranes].
abstract: OBJECTIVE
  To explore surface roughness of bone cement and surround tissue on histological characteristic of induced membranes.
  
  
  METHODS
  Bone cements with smooth and rough surface were implanted in radius bone defect, intramuscular and subcutaneous sites of rabbits, and formed induced membranes. Membranes were obtained and stained (HE) 6 weeks later. Images of membrane tissue were obtained and analyzed with an automated image analysis system. Five histological parameters of membranes were measured with thickness,area,cell density,ECM density and microvessel density. Double factor variance analysis was used to evaluate the effect of the two factors on histological characteristics of induced membranes.
  
  
  RESULTS
  Membranes can be induced by each kind of bone cement and at all the three tissue sites. In histological parameters of thickness,area and micro vessel,there were significant differences among the membranes induced at different tissue sites (P = 0.000, P = 0.000, P = 0.000); whereas, there were no significant differences in histological parameters of cell density and ECM density (P = 0.734, P = 0.638). In all five histological parameters of membranes, there were no significant differences between the membranes induced by bone cements with different surface roughness (P = 0.506, P = 0.185, P = 0.883, P = 0.093, P = 0.918).
  
  
  CONCLUSIONS
  Surround tissue rather than surface roughness of bone cements can affect the histological characteristics of induced membranes. The fibrocystic number, vascularity, mechanical tension and micro motion of the surround tissue may be closely correlated with the histological characteristics of induced membranes.
authors: Hai-Xiao
authors: HX
authors: Liu
authors: Hua-Zi
authors: HZ
authors: Xu
authors: Yu
authors: Y
authors: Zhang
authors: Gang
authors: G
authors: Hu
authors: Yue
authors: Y
authors: Shen
authors: Xiao-Jie
authors: XJ
authors: Cheng
authors: Lei
authors: L
authors: Peng
mesh_terms: D000818:Animals; D001843:Bone Cements; D005260:Female; D008566:Membranes / Q000166:cytology*; D011817:Rabbits; D011884:Radius / Q000166:cytology; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001843:Bone Cements