title: Serial crystallography on in vivo grown microcrystals using synchrotron radiation.
abstract: Crystal structure determinations of biological macromolecules are limited by the availability of sufficiently sized crystals and by the fact that crystal quality deteriorates during data collection owing to radiation damage. Exploiting a micrometre-sized X-ray beam, high-precision diffractometry and shutterless data acquisition with a pixel-array detector, a strategy for collecting data from many micrometre-sized crystals presented to an X-ray beam in a vitrified suspension is demonstrated. By combining diffraction data from 80 Trypanosoma brucei procathepsin B crystals with an average volume of 9 µm(3), a complete data set to 3.0 Å resolution has been assembled. The data allowed the refinement of a structural model that is consistent with that previously obtained using free-electron laser radiation, providing mutual validation. Further improvements of the serial synchrotron crystallography technique and its combination with serial femtosecond crystallography are discussed that may allow the determination of high-resolution structures of micrometre-sized crystals.
authors: Center for Free-Electron Laser Science (CFEL), Deutsches Elektronensynchrotron (DESY) , Notkestrasse 85, 22607 Hamburg, Germany.
authors: Cornelius
authors: C
authors: Gati
authors: European Molecular Biology Laboratory (EMBL), Hamburg Outstation , Notkestrasse 85, 22607 Hamburg, Germany.
authors: Gleb
authors: G
authors: Bourenkov
authors: Joint Laboratory for Structural Biology of Infection and Inflammation, Institute of Biochemistry and Molecular Biology, University of Hamburg, and Institute of Biochemistry, University of Lübeck , Notkestrasse 85, 22607 Hamburg, Germany.
authors: Marco
authors: M
authors: Klinge
authors: Joint Laboratory for Structural Biology of Infection and Inflammation, Institute of Biochemistry and Molecular Biology, University of Hamburg, and Institute of Biochemistry, University of Lübeck , Notkestrasse 85, 22607 Hamburg, Germany.
authors: Dirk
authors: D
authors: Rehders
authors: Center for Free-Electron Laser Science (CFEL), Deutsches Elektronensynchrotron (DESY) , Notkestrasse 85, 22607 Hamburg, Germany.
authors: Francesco
authors: F
authors: Stellato
authors: Center for Free-Electron Laser Science (CFEL), Deutsches Elektronensynchrotron (DESY) , Notkestrasse 85, 22607 Hamburg, Germany ; Institute of Biochemistry and Molecular Biology, University of Hamburg , Notkestrasse 85, 22607 Hamburg, Germany.
authors: Dominik
authors: D
authors: Oberthür
authors: Center for Free-Electron Laser Science (CFEL), Deutsches Elektronensynchrotron (DESY) , Notkestrasse 85, 22607 Hamburg, Germany.
authors: Oleksandr
authors: O
authors: Yefanov
authors: Institute of Biochemistry and Molecular Biology, University of Hamburg , Notkestrasse 85, 22607 Hamburg, Germany ; Interfaculty Institute of Biochemistry, University of Tübingen , Hoppe-Seyler-Strasse 4, 72076 Tübingen, Germany.
authors: Benjamin P
authors: BP
authors: Sommer
authors: Interfaculty Institute of Biochemistry, University of Tübingen , Hoppe-Seyler-Strasse 4, 72076 Tübingen, Germany.
authors: Stefan
authors: S
authors: Mogk
authors: Interfaculty Institute of Biochemistry, University of Tübingen , Hoppe-Seyler-Strasse 4, 72076 Tübingen, Germany.
authors: Michael
authors: M
authors: Duszenko
authors: Institute of Biochemistry and Molecular Biology, University of Hamburg , Notkestrasse 85, 22607 Hamburg, Germany.
authors: Christian
authors: C
authors: Betzel
authors: European Molecular Biology Laboratory (EMBL), Hamburg Outstation , Notkestrasse 85, 22607 Hamburg, Germany.
authors: Thomas R
authors: TR
authors: Schneider
authors: Center for Free-Electron Laser Science (CFEL), Deutsches Elektronensynchrotron (DESY) , Notkestrasse 85, 22607 Hamburg, Germany ; Institute of Experimental Physics, University of Hamburg , Luruper Chaussee 149, 22761 Hamburg, Germany.
authors: Henry N
authors: HN
authors: Chapman
authors: Joint Laboratory for Structural Biology of Infection and Inflammation, Institute of Biochemistry and Molecular Biology, University of Hamburg, and Institute of Biochemistry, University of Lübeck , Notkestrasse 85, 22607 Hamburg, Germany.
authors: Lars
authors: L
authors: Redecke
keywords: in vivo grown microcrystals; protein microcrystallography; serial crystallography