title: MicroRNA expression profile of bromocriptine-resistant prolactinomas.
abstract: MicroRNAs (miRNA) have been implicated in the resistance of tumors to chemotherapy. However, little is known about miRNA expression in bromocriptine-resistant prolactinomas. In this study, 23 prolactinoma samples were classified as bromocriptine-sensitive or -resistant according to the clinical definition of bromocriptine resistance, and their miRNA expression profiles were determined using Solexa sequencing. We found 41 miRNAs that were differentially expressed between the two groups, and 12 of these were validated by stem-loop qRT-PCR. Hsa-mir-93, hsa-mir-17, hsa-mir-22*, hsa-mir-126*, hsa-mir-142-3p, hsa-mir-144*, hsa-mir-486-5p, hsa-mir-451, and hsa-mir-92a were up-regulated and hsa-mir-30a, hsa-mir-382, and hsa-mir-136 were down-regulated in bromocriptine-resistant prolactinomas in comparison with bromocriptine-sensitive prolactinomas. Furthermore, silencing of mir-93 significantly increased the sensitivity of MMQ cells to dopamine agonist treatment. Mir-93 directly affected p21 expression in MMQ cells by targeting the 3'-UTR. Our study is the first to identify a miRNA expression profile associated with bromocriptine-resistant prolactinoma.
authors: Department of Neurosurgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address: zhebaowu@aliyun.com.
authors: Zhe Bao
authors: ZB
authors: Wu
authors: Department of Neurosurgery, Yuyao People's Hospital, Ningbo 315400, China.
authors: Wei Qiang
authors: WQ
authors: Li
authors: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors: Shao Jian
authors: SJ
authors: Lin
authors: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors: Cheng De
authors: CD
authors: Wang
authors: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors: Lin
authors: L
authors: Cai
authors: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors: Jiang Long
authors: JL
authors: Lu
authors: Department of Neurosurgery, Jinhua People's Hospital, Jinhua 321000, China.
authors: Yun Xiang
authors: YX
authors: Chen
authors: Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors: Zhi Peng
authors: ZP
authors: Su
authors: Department of Neurosurgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors: Han Bing
authors: HB
authors: Shang
authors: Department of Neurosurgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors: Wen Lei
authors: WL
authors: Yang
authors: Department of Neurosurgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: rjneurosurgery@qq.com.
authors: Wei Guo
authors: WG
authors: Zhao
mesh_terms: D000328:Adult; D001971:Bromocriptine / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D006727:Hormone Antagonists / Q000494:pharmacology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D015175:Prolactinoma / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D006727:Hormone Antagonists; D035683:MicroRNAs; D012334:RNA, Neoplasm; D001971:Bromocriptine
keywords: Bromocriptine; Prolactinoma; Resistance; microRNA; microRNA-93; p21