title: The interaction of CuInS2 /ZnS/TGA quantum dots with tyrosine kinase inhibitor and its application.
abstract: The interactions between thioglycolic acid-capped-CuInS2 /ZnS quantum dots (CuInS2 /ZnS/TGA QDs) and tyrosine kinase inhibitor (TKI) were investigated using fluorescence, ultraviolet-visible spectrometry and Fourier transform infrared spectrometry. The results indicated that the fluorescence intensity of CuInS2 /ZnS/TGA could be quenched by imatinib, dasatinib, nilotinib, gefitinib and erlotinib, which hinted that CuInS2 /ZnS/TGA QDs could be used in the detection of TKI in active pharmaceutical ingredients (API). Calibration curves showed good linear correlation and low detection limits. The average recovery was between 98 and 102%. Moreover, the nature of the fluorescence quenching mechanism of CuInS2 /ZnS/TGA QDs by TKI was discussed. A ground state complex was formed by hydrogen bonding between the carboxyl group of CuInS2 /ZnS/TGA QDs and the amino group of TKI. This led to an increase in non-radiative transition and fluorescence quenching of CuInS2 /ZnS/TGA QDs.
authors: Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing, 211198, China; Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 211198, China.
authors: Shenghua
authors: S
authors: Liao
authors: Yu
authors: Y
authors: Huang
authors: Jiaxin
authors: J
authors: Zuo
authors: Zhengyu
authors: Z
authors: Yan
mesh_terms: D001665:Binding Sites; D003300:Copper / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006860:Hydrogen Bonding; D007204:Indium / Q000737:chemistry; D047428:Protein Kinase Inhibitors / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013440:Sulfides / Q000737:chemistry*; D013696:Temperature; D013864:Thioglycolates / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D047428:Protein Kinase Inhibitors; D013440:Sulfides; D013864:Thioglycolates; D017967:Zinc Compounds; D007204:Indium; C017487:2-mercaptoacetate; D003300:Copper; D011505:Protein-Tyrosine Kinases; C031238:zinc sulfide
keywords: content determination; interaction; intermolecular hydrogen bond; thioglycolic acid-capped-CuInS2/ZnS quantum dots; tyrosine kinase inhibitor