title: Development of a highly sensitive MIP based-QCM nanosensor for selective determination of cholic acid level in body fluids.
abstract: Determination of cholic acid is very important and necessary in body fluids due to its both pharmaceutical and clinical significance. In this study, a quartz crystal microbalance (QCM) nanosensor, which is imprinted cholic acid, has been developed for the assignation of cholic acid. The cholic acid selective memories have been generated on QCM electrode surface by using molecularly imprinted polymer (MIP) based on methacryloylamidohistidine-copper (II) (MAH-Cu(II)) pre-organized monomer. The cholic acid imprinted nanosensor was characterized by atomic force microscopy (AFM) and then analytical performance of the cholic acid imprinted QCM nanosensor was studied. The detection limit was found to be 0.0065μM with linear range of 0.01-1,000 μM. Moreover, the high value of Langmuir constant (b) (7.3*10(5)) obtained by Langmuir graph showed that the cholic acid imprinted nanosensor had quite strong binding sites affinity. At the last step of this procedure, cholic acid levels in body fluids were determined by the prepared imprinted QCM nanosensor.
authors: Karamanoğlu Mehmetbey University, Faculty of Engineering, Department of Energy Systems Engineering, Karaman 70200, Turkey. Electronic address: aysari@yahoo.com.
authors: Aytaç
authors: A
authors: Gültekin
authors: Karamanoğlu Mehmetbey University, Faculty of Engineering, Department of Energy Systems Engineering, Karaman 70200, Turkey.
authors: Gamze
authors: G
authors: Karanfil
authors: Karamanoğlu Mehmetbey University, Faculty of Engineering, Department of Materials Science and Engineering, Karaman 70200, Turkey.
authors: Savaş
authors: S
authors: Sönmezoğlu
authors: Anadolu University, Faculty of Science, Department of Chemistry, Eskişehir 26470, Turkey.
authors: Rıdvan
authors: R
authors: Say
mesh_terms: D019826:Cholic Acid / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D006801:Humans; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D054802:Molecular Imprinting / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D058525:Quartz Crystal Microbalance Techniques / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
chemical_list: D019826:Cholic Acid
keywords: Cholic acid; MIP; Nanosensor; QCM