title: IgG sera levels against a subset of periodontopathogens and severity of disease in aggressive periodontitis patients: a cross-sectional study of selected pocket sites.
abstract: OBJECTIVE
  To evaluate the association among serum immunoglobulin G (IgG) responses to Aggregatibacter actinomycetemcomitans (Aa) serotypes a, b and c, Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf) and clinical parameters in Aggressive Periodontitis (AP) subjects. Associations between periodontal pathogens and clinical and immunological parameters were also evaluated.
  
  
  METHODS
  Thirty-eight subjects diagnosed with generalized AP (GAP) and localized AP (LAP) were included. Ten healthy controls were also evaluated. Clinical parameters were assessed and percentages of subgingival levels of Aa, Pg and Tf (beyond bacterial load), were determined by quantitative real-time polymerase chain reaction. Serum IgG antibody levels against Aa, Pg and Tf were evaluated by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Percentages of Aa, Pg and Tf were significantly higher in AP than in controls. The response to Aa serotype c was higher in LAP subjects than in controls. There were no differences in microbial composition or antibodies responses between GAP and LAP, except for IgG response to Tf. Pg levels were correlated with probing depth (PD), BoP and CAL in GAP but not in LAP subjects. Tf levels correlated with PD and CAL in GAP subjects. In GAP, the infection levels of Aa and Pg correlated with the corresponding IgG levels to Aa serotype c and Pg.
  
  
  CONCLUSIONS
  Given the evidences that IgG response in AP patients correlated with bacterial infection level in GAP, but not in LAP, and that LAP patients lack a response to Tf, despite harbouring this species, our data suggest a difference in host immune defence between these two forms of aggressive periodontitis.
authors: Department of Periodontology, Dental School, University of São Paulo, São Paulo, SP, Brazil.
authors: Luciana
authors: L
authors: Saraiva
authors: Estela S
authors: ES
authors: Rebeis
authors: Eder de S
authors: Ede S
authors: Martins
authors: Ricardo T
authors: RT
authors: Sekiguchi
authors: Ellen S
authors: ES
authors: Ando-Suguimoto
authors: Carlos Eduardo S
authors: CE
authors: Mafra
authors: Marinella
authors: M
authors: Holzhausen
authors: Giuseppe A
authors: GA
authors: Romito
authors: Marcia P A
authors: MP
authors: Mayer
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification / Q000276:immunology; D010520:Aggressive Periodontitis / Q000145:classification / Q000276:immunology / Q000382:microbiology*; D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood*; D058491:Bacterial Load; D001439:Bacteroides / Q000145:classification / Q000276:immunology; D003430:Cross-Sectional Studies; D005260:Female; D006090:Gram-Negative Bacteria / Q000276:immunology*; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D017622:Periodontal Attachment Loss / Q000276:immunology / Q000382:microbiology; D010514:Periodontal Pocket / Q000276:immunology / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000145:classification / Q000276:immunology; D011859:Radiography; D065288:Serogroup; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G
keywords: Aggregatibacter actinomycetemcomitans; Porphyromonas gingivalis; Tannerella forsythia; aggressive periodontitis; antibodies; qPCR