title: Effects of growth hormone gene polymorphism on lipogenic gene expression levels in diaphragm tissues of Japanese black heifers.
abstract: Two SNPs, i.e. L127V and T172M, of bovine growth hormone (GH) causing the presence of GH gene haplotypes A, B, and C was previously shown to alter intramuscular fatty acid (FA) composition in Japanese Black (JB) heifers. To determine the SNP effect on somatotropic hormone concentration and lipogenesis, we measured plasma GH, insulin, and insulin-like growth factor-1 (IGF-1) concentrations. We also measured mRNA levels of fatty acid synthase (FASN), stearoyl-coA desaturase (SCD), and sterol regulatory element binding proteins-1 (SREBP-1) and FA composition in diaphragm tissues. Heifers with genotype CC had the lowest plasma insulin concentration and FASN and SCD mRNA levels among genotypes. FASN mRNA levels in haplotype A tended to positively correlate with saturated FA (SFA) content and negatively correlated with C18:2 and unsaturated FA (USFA) contents. SCD mRNA levels in haplotype A positively correlated with monounsaturated FA (MUFA) contents and negatively correlated with C18:0 content. They also tended to positively correlate with C16:1, C18:1, and USFA contents and USFA/SFA ratio and negatively correlate with SFA content. Taken together, GH gene polymorphism affects the lipogenic genes expression levels and their relationships with fatty acid compositions in diaphragm tissues of JB heifers at 31 months of age.
authors: National Livestock Breeding Center, Fukushima 961-8511, Japan .
authors: Astrid
authors: A
authors: Ardiyanti
authors: National Livestock Breeding Center, Fukushima 961-8511, Japan .
authors: Tsuyoshi
authors: T
authors: Abe
authors: National Livestock Breeding Center, Fukushima 961-8511, Japan .
authors: Nanae
authors: N
authors: Tameoka
authors: National Livestock Breeding Center, Fukushima 961-8511, Japan .
authors: Eiji
authors: E
authors: Kobayashi
authors: Yamagata General Agricultural Research Center, Shinjo 996-0041, Japan .
authors: Noriaki
authors: N
authors: Shoji
authors: National Livestock Breeding Center, Fukushima 961-8511, Japan .
authors: Yoshihisa
authors: Y
authors: Ohtani
authors: Department of Animal Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan .
authors: Keiichi
authors: K
authors: Suzuki
authors: National Livestock Breeding Center, Fukushima 961-8511, Japan .
authors: Sang-Gun
authors: SG
authors: Roh
authors: National Livestock Breeding Center, Fukushima 961-8511, Japan .
authors: Kazuo
authors: K
authors: Katoh
keywords: Fatty Acid Synthase; GH Gene Polymorphism; Gene Expression Levels; Japanese Black Heifers; Stearoyl-coA Desaturase; Sterol Regulatory Element Binding Proteins-1