title: Polyhydroxyalkanoate biosynthesis and simultaneous remotion of organic inhibitors from sugarcane bagasse hydrolysate by Burkholderia sp.
abstract: Burkholderia sp. F24, originally isolated from soil, was capable of growth on xylose and removed organic inhibitors present in a hemicellulosic hydrolysate and simultaneously produced poly-3-hydroxybutyrate (P3HB). Using non-detoxified hydrolysate, Burkholderia sp. F24 reached a cell dry weight (CDW) of 6.8 g L(-1), containing 48 % of P3HB and exhibited a volumetric productivity (PP3HB) of 0.10 g L(-1) h(-1). Poly-3-hydroxybutyrate-co-3-hydroxyvalerate copolymers (P3HB-co-3HV) were produced using xylose and levulinic acid (LA) as carbon sources. In shake flask cultures, the 3HV content in the copolymer increased from 9 to 43 mol% by adding LA from 1.0 to 5.0 g L(-1). In high cell density cultivation using concentrated hemicellulosic hydrolysate F24 reached 25.04 g L(-1) of CDW containing 49 % of P3HB and PP3HB of 0.28 g L(-1 )h(-1). Based on these findings, second-generation ethanol and bioplastics from sugarcane bagasse is proposed.
authors: Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, Cidade Universitária, São Paulo, SP, CEP 05508-000, Brazil.
authors: Mateus Schreiner Garcez
authors: MS
authors: Lopes
authors: José Gregório Cabrera
authors: JG
authors: Gomez
authors: Marilda Keico
authors: MK
authors: Taciro
authors: Thatiane Teixeira
authors: TT
authors: Mendonça
authors: Luiziana Ferreira
authors: LF
authors: Silva
mesh_terms: D019117:Burkholderia / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D031786:Saccharum / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D012988:Soil Microbiology; D014994:Xylose / Q000378:metabolism
chemical_list: D054813:Polyhydroxyalkanoates; D002482:Cellulose; C027433:bagasse; D014994:Xylose