title: Association of abdominal obesity, insulin resistance, and oxidative stress in adipose tissue in women with polycystic ovary syndrome.
abstract: OBJECTIVE
  To study the expression of insulin signaling-related genes and oxidative stress markers in the visceral adipose tissue obtained from polycystic ovary syndrome (PCOS) patients and healthy control subjects and to investigate the relationships among abdominal obesity, insulin resistance, and oxidative stress at the tissue level.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  University teaching hospital.
  
  
  METHODS
  In total, 30 PCOS patients and 30 healthy control subjects, who underwent laparoscopic surgery, were included in the study.
  
  
  METHODS
  Abdominal obesity was defined based on waist circumference (WC). The homeostasis model index was used to assess insulin resistance (HOMA-IR).
  
  
  METHODS
  Gene expression of glucose transporter 4 (GLUT4) and insulin receptor substrate 1 (IRS1) in visceral adipose tissue (VAT) and the parameters of oxidative stress, such as superoxide dismutase, enzyme glutathione reductase, and dimethylarginine, were measured, and the expression of protein oxidative damage product 3-nitro-tyrosine residues (nitrotyrosine) in VAT was identified with the use of immunohistochemistry.
  
  
  RESULTS
  PCOS was associated with lower expression of GLUT4 and IRS1 and a higher level of oxidative stress in VAT, which was strongly correlated with WC and HOMA-IR. Presence of abdominal obesity further intensified the correlations observed in our measurements. The nitrotyrosine expression in VAT was stronger in PCOS patients.
  
  
  CONCLUSIONS
  The strong correlation of insulin resistance with oxidative stress at the VAT level suggests that local oxidative stress and abnormalities of insulin signaling in adipose tissue play critical roles in the pathogenesis of PCOS.
authors: Department of Obstetrics and Gynecology, Sichuan University-Chinese University of Hong Kong, Hong Kong, People's Republic of China.
authors: Li
authors: L
authors: Chen
authors: Joint Laboratory for Reproductive Medicine, Sichuan University-Chinese University of Hong Kong, Hong Kong, People's Republic of China; Key Laboratory of Obstetric, Gynecologic, and Pediatric Diseases and Birth Defects, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China. Electronic address: xuwenming1973@163.com.
authors: Wen Ming
authors: WM
authors: Xu
authors: Department of Obstetrics and Gynecology, Sichuan University-Chinese University of Hong Kong, Hong Kong, People's Republic of China.
authors: Dan
authors: D
authors: Zhang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D005260:Female; D051275:Glucose Transporter Type 4 / Q000235:genetics; D006785:Hospitals, University; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000737:chemistry*; D016014:Linear Models; D015999:Multivariate Analysis; D056128:Obesity, Abdominal / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D011085:Polycystic Ovary Syndrome / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction; D055105:Waist Circumference; D055815:Young Adult
chemical_list: D015415:Biomarkers; D051275:Glucose Transporter Type 4; C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D012333:RNA, Messenger; C495692:SLC2A4 protein, human
keywords: Polycystic ovary syndrome (PCOS); abdominal obesity; insulin resistance; oxidative stress; visceral adipose tissue (VAT); waist circumference (WC)