title: Anthocyanins from fermented berry beverages inhibit inflammation-related adiposity response in vitro.
abstract: Increased adiposity has been associated with macrophage infiltration into the adipose tissue which, in turn, leads to obesity comorbidities, including type 2 diabetes. The objective of this study was to evaluate the effect of anthocyanin (ANC)-enriched fractions from blackberry-blueberry beverages on inflammation and adipogenesis in an in vitro model of inflammation mimicking the pathologic interaction between adipocytes and macrophages. Blend ANCs inhibited secretion of nitric oxide (17.5%), tumor necrosis factor-alpha (TNF-α) (89.4%), and phosphorylated-p65 nuclear factor kappa-B (52.1%) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages after 24 h. Blends reduced intracellular fat accumulation (28.2%) when applied during 3T3-L1 adipocyte differentiation and inhibited isoproterenol-induced lipolysis (18.6%) of mature 3T3-L1 cells. In addition, blend ANCs restored adiponectin-blunted gene expression induced by the TNF-α treatment (18.2%) and reduced the glycerol release (15.9%) induced by LPS-induced macrophage-conditioned media (CM) in adipocytes. Furthermore, blends slightly restored the insulin-induced glucose uptake of adipocytes, blunted by the CM treatment. In conclusion, ANCs from blueberry and blackberry dealcoholized fermented beverages are potential inhibitors of inflammation-related adiposity response and sensitizers of insulin signaling in adipocytes.
authors: 1 Department of Nutrition, School of Medicine, University of Chile , Santiago, Chile .
authors: Diego F
authors: DF
authors: Garcia-Diaz
authors: Michelle H
authors: MH
authors: Johnson
authors: Elvira G
authors: EG
authors: de Mejia
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D050156:Adipogenesis / Q000187:drug effects; D050154:Adiposity / Q000187:drug effects; D000818:Animals; D000872:Anthocyanins / Q000378:metabolism / Q000494:pharmacology*; D001628:Beverages / Q000032:analysis* / Q000382:microbiology; D029798:Blueberry Plants / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D005285:Fermentation; D005638:Fruit / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D008066:Lipolysis; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000276:immunology; D000067996:RAW 264.7 Cells; D065856:Rubus / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D012440:Saccharomyces / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D000872:Anthocyanins; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide
keywords: adiposity; anthocyanins; anti-inflammatory; diabetes; fermentation