title: [Comparative resistance of typical and genetically altered Vibrio cholerae biovar El Tor strains to the effect of unfavorable environmental factors].
abstract: OBJECTIVE
  Carry out comparative analysis of survival of typical strains and genovariants of V. cholerae biovar El Tor imported in different years to the territory of Russian Federation, in the absence of nutrients and under the conditions of temperature stress.
  
  
  METHODS
  24 V. cholerae biovar El Tor strains isolated in 1970 - 2011 were studied, 8 of those were typical isolates and 16--genetically altered variants. Strain survival was studied in 0.9% sodium chloride solution and autoclaved river water at various temperature modes (5, 25, 37 and 42 degrees C). Protein composition and exopolysaccharide production were determined by electrophoresis method by U.K. Laemmli.
  
  
  RESULTS
  Genovariants as well as typical strains were shown to be able to exist for a long time (up to 5 months) in the absence of nutrients at the temperature of 25 degrees C. However, unlike typical eltor vibrios, genovariants were more resistant to temperature stress. As a result of adaptation to high temperature (42 degrees C) biosynthesis of porin proteins of outer membrane OmpU and/or OmpT is increased in genovariant cells, and at lower temperatures (5 degrees C)--exopolysaccharide.
  
  
  CONCLUSIONS
  V. cholerae biovar El Tor genovariants are able to adapt to temperature change better, that may facilitate their higher survival in the environment.
authors: S P
authors: SP
authors: Zadnova
authors: D A
authors: DA
authors: Agafonov
authors: A V
authors: AV
authors: Shashkova
authors: N I
authors: NI
authors: Smirnova
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D018272:Porins / Q000096:biosynthesis / Q000235:genetics*; D012426:Russia; D013312:Stress, Physiological; D013696:Temperature; D014734:Vibrio cholerae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; C096349:OmpU protein, Vibrio cholerae; D011135:Polysaccharides, Bacterial; D018272:Porins; C405564:ompT protein, bacteria