title: FADD is essential for glucose uptake and survival of thymocytes.
abstract: Fas-associated protein with death domain (FADD) has been implicated in T lymphocytes, but the nature of FADD-dependent mechanism in early T cell development has not been completely elucidated. In this study, using T-cell specific deletion mice, we observed that FADD deficiency in thymocytes led to increased apoptosis and reduced cell numbers, which may be attributed to the reduction of Glut1 expression and correspondingly decreased glucose uptake. Furthermore, an abnormal transduction of Akt signaling was discovered in FADD(-/-) thymocytes, which may be responsible for the declined Glut1 expression. Collectively, our results demonstrate the new function of FADD in glucose metabolism and survival of early T cells.
authors: State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences and School of Stomatology, Affiliated Stomatological Hospital, Nanjing University, Nanjing 210093, Jiangsu, PR China.
authors: Xiang-Yu
authors: XY
authors: Zhang
authors: State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences and School of Stomatology, Affiliated Stomatological Hospital, Nanjing University, Nanjing 210093, Jiangsu, PR China.
authors: Bing-Ya
authors: BY
authors: Yang
authors: State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences and School of Stomatology, Affiliated Stomatological Hospital, Nanjing University, Nanjing 210093, Jiangsu, PR China.
authors: Jia-Yu
authors: JY
authors: Wang
authors: State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences and School of Stomatology, Affiliated Stomatological Hospital, Nanjing University, Nanjing 210093, Jiangsu, PR China.
authors: Xuan
authors: X
authors: Mo
authors: State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences and School of Stomatology, Affiliated Stomatological Hospital, Nanjing University, Nanjing 210093, Jiangsu, PR China.
authors: Jing
authors: J
authors: Zhang
authors: State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences and School of Stomatology, Affiliated Stomatological Hospital, Nanjing University, Nanjing 210093, Jiangsu, PR China; Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target-Pharma Laboratories Inc., Changzhou 213164, Jiangsu, PR China. Electronic address: huazc@nju.edu.cn.
authors: Zi-Chun
authors: ZC
authors: Hua
mesh_terms: D000818:Animals; D017209:Apoptosis; D001693:Biological Transport, Active; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D053200:Fas-Associated Death Domain Protein / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*
chemical_list: C505534:Fadd protein, mouse; D053200:Fas-Associated Death Domain Protein; D051272:Glucose Transporter Type 1; C495678:Slc2a1 protein, mouse; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose
keywords: Akt; FADD; Glucose uptake; Glut1; Thymocytes