title: Evidence of Borrelia in wild and domestic mammals from the state of Minas Gerais, Brazil.
abstract: The main of the study was to evaluate the presence of Borrelia burgdorferi infection in domestic and wild vertebrates and ectoparasites in endemic areas from the state of Minas Gerais, Brazil. A total of 445 serum samples were examined by ELISA, which used the Borrelia burgdorferi strain G39/40 U.S. source and 3,821 tick samples were tested by polymerase chain reaction (PCR). B. burgdorferi antibodies were found in 30 serum samples (6.74%); three in marsupials (7.69%), three in rodents (2.80%), nine in dogs (6.25%), and 15 in horses (9.68%). Nested-PCR performed in DNA samples obtained from collected ticks demonstrated negative results. Although attempts to amplify B. burgdorferi DNA from ticks had been not successful, the presence of seroreactive vertebrates suggests the possibility the Borrelia species circulating in these regions. Further research is required to provide information on the presence of Borrelia in Brazilian territory and its association with Baggio-Yoshinari syndrome.
authors: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa ? UFV, Viçosa, MG, Brazil.
authors: Carlos Emmanuel
authors: CE
authors: Montandon
authors: Faculdade de Medicina, Universidade de São Paulo ? USP, São Paulo, SP, Brazil.
authors: Natalino Hajime
authors: NH
authors: Yoshinari
authors: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa ? UFV, Viçosa, MG, Brazil.
authors: Bruno Silva
authors: BS
authors: Milagres
authors: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa ? UFV, Viçosa, MG, Brazil.
authors: Rafael
authors: R
authors: Mazioli
authors: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa ? UFV, Viçosa, MG, Brazil.
authors: Gabriel Guimarães
authors: GG
authors: Gomes
authors: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa ? UFV, Viçosa, MG, Brazil.
authors: Higo Nasser
authors: HN
authors: Moreira
authors: Departamento de Ciências Médicas, Universidade Federal de Ouro Preto ? UFOP, Ouro Preto, MG, Brazil.
authors: Amanda de Freitas
authors: Ade F
authors: Padilha
authors: Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo ? USP, São Paulo, SP, Brazil.
authors: Guido Gomes
authors: GG
authors: Wanderley
authors: Faculdade de Medicina, Universidade de São Paulo ? USP, São Paulo, SP, Brazil.
authors: Elenice
authors: E
authors: Mantovani
authors: Departamento de Ciências Médicas, Universidade Federal de Ouro Preto ? UFOP, Ouro Preto, MG, Brazil.
authors: Márcio Antônio Moreira
authors: MA
authors: Galvão
authors: Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo ? USP, São Paulo, SP, Brazil.
authors: Helio
authors: H
authors: Langoni
authors: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa ? UFV, Viçosa, MG, Brazil.
authors: Cláudio
authors: C
authors: Mafra
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D000835:Animals, Wild / Q000382:microbiology*; D025065:Borrelia burgdorferi / Q000302:isolation & purification*; D001938:Brazil; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008193:Lyme Disease / Q000175:diagnosis / Q000662:veterinary*; D008394:Marsupialia / Q000382:microbiology*; D012377:Rodentia / Q000382:microbiology*