title: Novel divergent nidovirus in a python with pneumonia.
abstract: The order Nidovirales contains large, enveloped viruses with a non-segmented positive-stranded RNA genome. Nidoviruses have been detected in man and various animal species, but, to date, there have been no reports of nidovirus in reptiles. In the present study, we describe the detection, characterization, phylogenetic analyses and disease association of a novel divergent nidovirus in the lung of an Indian python (Python molurus) with necrotizing pneumonia. Characterization of the partial genome (>33 000 nt) of this virus revealed several genetic features that are distinct from other nidoviruses, including a very large polyprotein 1a, a putative ribosomal frameshift signal that was identical to the frameshift signal of astroviruses and retroviruses and an accessory ORF that showed some similarity with the haemagglutinin-neuraminidase of paramyxoviruses. Analysis of genome organization and phylogenetic analysis of polyprotein 1ab suggests that this virus belongs to the subfamily Torovirinae. Results of this study provide novel insights into the genetic diversity within the order Nidovirales.
authors: Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.
authors: Rogier
authors: R
authors: Bodewes
authors: Department of Pathology, University of Veterinary Medicine, Hannover, Germany.
authors: Charlotte
authors: C
authors: Lempp
authors: Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.
authors: Anita C
authors: AC
authors: Schürch
authors: Department of Pathology, University of Veterinary Medicine, Hannover, Germany.
authors: Andre
authors: A
authors: Habierski
authors: Department of Pathology, University of Veterinary Medicine, Hannover, Germany.
authors: Kerstin
authors: K
authors: Hahn
authors: Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.
authors: Mart
authors: M
authors: Lamers
authors: Hannover Adventure Zoo, Hannover, Germany.
authors: Katja
authors: K
authors: von Dörnberg
authors: Department of Pathology, University of Veterinary Medicine, Hannover, Germany.
authors: Peter
authors: P
authors: Wohlsein
authors: Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.|Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.
authors: Jan Felix
authors: JF
authors: Drexler
authors: Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.
authors: Bart L
authors: BL
authors: Haagmans
authors: Viroclinics Biosciences BV, Rotterdam, The Netherlands.|Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.
authors: Saskia L
authors: SL
authors: Smits
authors: Department of Pathology, University of Veterinary Medicine, Hannover, Germany.
authors: Wolfgang
authors: W
authors: Baumgärtner
authors: Artemis Research Institute for Wildlife Health, Utrecht, The Netherlands.|Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.|Viroclinics Biosciences BV, Rotterdam, The Netherlands.|Centre for Infection Medicine and Zoonoses Research, University of Veterinary Medicine, Hannover, Germany.
authors: Albert D M E
authors: ADME
authors: Osterhaus
mesh_terms: D000818:Animals; D001483:Base Sequence; D017821:Boidae / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral; D008168:Lung / Q000473:pathology / Q000821:virology; D008969:Molecular Sequence Data; D028381:Nidovirales / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D030341:Nidovirales Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D011024:Pneumonia, Viral / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins