title: Whole-genome analyses reveals the animal origin of a rotavirus G4P[6] detected in a child with severe diarrhea.
abstract: Group A rotaviruses are a major cause of severe gastroenteritis in children worldwide. Currently, two rotavirus vaccines are being used in vaccination programs, and one of the factors involved in lower vaccine efficacy is the mismatch among the circulating strains and the vaccine strains. Thus, the emergence of animal strains in the human population could affect the efficacy of vaccination programs. Here we report the presence of a G4P[6] strain in a Paraguayan child presenting acute gastroenteritis in 2009. Genomic analyses revealed that the strain presents a porcine-like genome (G4-P[6]-I1-R1-C1-M1-A8-N1-T7-E1-H1), suggesting a direct animal-to-human transmission. Continuous surveillance of rotaviruses in humans and animals will help us to better understand rotavirus epidemiology and evolution.
authors: Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay.
authors: Magaly
authors: M
authors: Martinez
authors: Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay.
authors: Maria E
authors: ME
authors: Galeano
authors: The J. Craig Venter Institute, Rockville, MD, USA.
authors: Asmik
authors: A
authors: Akopov
authors: Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay.
authors: Ruth
authors: R
authors: Palacios
authors: Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay.
authors: Graciela
authors: G
authors: Russomando
authors: The J. Craig Venter Institute, Rockville, MD, USA.
authors: Ewen F
authors: EF
authors: Kirkness
authors: Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay. Electronic address: gabriel_parra@hotmail.com.
authors: Gabriel I
authors: GI
authors: Parra
mesh_terms: D000818:Animals; D002648:Child; D003967:Diarrhea / Q000821:virology*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D016679:Genome, Viral; D023281:Genomics; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D062486:Public Health Surveillance; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D012400:Rotavirus Infections / Q000635:transmission / Q000821:virology*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000939:Epitopes; D014764:Viral Proteins
keywords: Diarrhea; G4; Paraguay; Rotavirus; Zoonosis