title: Nanosecond pulsed electric fields (nsPEFs) regulate phenotypes of chondrocytes through Wnt/β-catenin signaling pathway.
abstract: Nanosecond pulsed electric fields (nsPEFs) characterized by high voltage, low energy and non-thermal effects, have been broadly investigated as a potential tumor therapy; however, little is known about their effects on somatic cells. In this current study, we evaluated effects of nsPEFs on the phenotype of chondrocytes (morphology, glycosaminoglycan (GAG) content, proliferation and gene expression) and explored the mechanisms involved. Our results demonstrated that exposing chondrocytes to nsPEFs led to enhanced proliferation and dedifferentiation, evidenced by the upregulated gene expression of collagen type I (COL I) and downregulated gene expression of Sox9, collagen type II (COL II) and aggrecan (AGG) with activation of the wnt/β-catenin signaling pathway. Inhibition of the wnt/β-catenin pathway partially blocked these effects. Thus we concluded that nsPEFs induce dedifferentiation of chondrocytes partially through transient activation of the wnt/β-catenin signaling pathway.
authors: Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors: Kun
authors: K
authors: Zhang
authors: Institute of Biomechaics and Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors: Jinsong
authors: J
authors: Guo
authors: 1] Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China [2] Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China [3] Arthritis Clinic and Research Center, Peking University People's Hospital, Beijing, 100871, China.
authors: Zigang
authors: Z
authors: Ge
authors: 1] Institute of Biomechaics and Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China [2] Center for BioMed-X Research, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
authors: Jue
authors: J
authors: Zhang
mesh_terms: D053674:Aggrecans / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D004558:Electric Stimulation; D006025:Glycosaminoglycans / Q000378:metabolism; D010641:Phenotype; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism; D013552:Swine; D013997:Time Factors; D015854:Up-Regulation; D051153:Wnt Proteins / Q000378:metabolism*; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D053674:Aggrecans; D024042:Collagen Type I; D024043:Collagen Type II; D006025:Glycosaminoglycans; D055758:SOX9 Transcription Factor; D051153:Wnt Proteins; D051176:beta Catenin