title: Fingerprinting of psychoactive drugs in zebrafish anxiety-like behaviors.
abstract: A major hindrance for the development of psychiatric drugs is the prediction of how treatments can alter complex behaviors in assays which have good throughput and physiological complexity. Here we report the development of a medium-throughput screen for drugs which alter anxiety-like behavior in adult zebrafish. The observed phenotypes were clustered according to shared behavioral effects. This barcoding procedure revealed conserved functions of anxiolytic, anxiogenic and psychomotor stimulating drugs and predicted effects of poorly characterized compounds on anxiety. Moreover, anxiolytic drugs all decreased, while anxiogenic drugs increased, serotonin turnover. These results underscore the power of behavioral profiling in adult zebrafish as an approach which combines throughput and physiological complexity in the pharmacological dissection of complex behaviors.
authors: Laboratório de Neurociências e Comportamento "Frederico Guilherme Graeff", Departamento de Morfologia e Ciências Fisiológicas, Centro de Ciências Biológicas e da Saúde, Universidade do Estado do Pará, Marabá, Pará, Brazil.
authors: Caio
authors: C
authors: Maximino
authors: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
authors: Annanda Waneza Batista
authors: AW
authors: da Silva
authors: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
authors: Juliana
authors: J
authors: Araújo
authors: Laboratório de Neurociências e Comportamento "Frederico Guilherme Graeff", Departamento de Morfologia e Ciências Fisiológicas, Centro de Ciências Biológicas e da Saúde, Universidade do Estado do Pará, Marabá, Pará, Brazil.
authors: Monica Gomes
authors: MG
authors: Lima
authors: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
authors: Vanessa
authors: V
authors: Miranda
authors: Laboratório de Neuroendocrinologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
authors: Bruna
authors: B
authors: Puty
authors: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
authors: Rancés
authors: R
authors: Benzecry
authors: Universidade Federal do Oeste do Pará, Oriximiná, Pará, Brazil.
authors: Domingos Luiz Wanderley
authors: DL
authors: Picanço-Diniz
authors: Laboratório de Neurociências e Comportamento, Núcleo de Pesquisa e Teoria do Comportamento, Universidade Federal do Pará, Belém, Pará, Brazil.
authors: Amauri
authors: A
authors: Gouveia
authors: Laboratório de Neuroendocrinologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
authors: Karen Renata Matos
authors: KR
authors: Oliveira
authors: Laboratório de Neuroendocrinologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
authors: Anderson Manoel
authors: AM
authors: Herculano
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001007:Anxiety / Q000188:drug therapy*; D002065:Buspirone / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D046352:Freezing Reaction, Cataleptic / Q000187:drug effects; D012701:Serotonin / Q000378:metabolism; D013550:Swimming; D015027:Zebrafish
chemical_list: D014151:Anti-Anxiety Agents; D012701:Serotonin; D002110:Caffeine; D003975:Diazepam; D002065:Buspirone