title: Isolation and characterization of microalgae for biodiesel production from Nisargruna biogas plant effluent.
abstract: Increasing energy demand and depleting fossil fuel sources have intensified the focus on biofuel production. Microalgae have emerged as a desirable source for biofuel production because of high biomass and lipid production from waste water source. In this study, five microalgae were isolated from effluents of Nisargruna biogas plants. These isolates were identified based on morphology and partial 18S and 23S rRNA gene sequences. Growth and lipid accumulation potential of these microalgae were investigated. One isolate, Chlorella sp. KMN3, accumulated high biomass (1.59 ± 0.05 g L(-1)) with moderate lipid content (20%), while another isolate Monoraphidium sp. KMN5 showed moderate biomass accumulation of 0.65 ± 0.05 g L(-1) with a very high (35%) lipid content. The fatty acid methyl esters mainly composed of C-16:0, C-18:0, C-18:1 and C-18:2. This observation makes these microalgae immensely potential candidate for biodiesel production using the effluent of a biogas plant as feed stock.
authors: Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors: Manisha
authors: M
authors: Tale
authors: Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India. Electronic address: ghoshsukh@rediffmail.com.
authors: Sukhendu
authors: S
authors: Ghosh
authors: Department of Microbiology, University of Pune, Pune 411007, India.
authors: Balasaheb
authors: B
authors: Kapadnis
authors: Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors: Sharad
authors: S
authors: Kale
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D005227:Fatty Acids / Q000378:metabolism; D007194:India; D008055:Lipids / Q000096:biosynthesis; D058086:Microalgae / Q000254:growth & development / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017423:Sequence Analysis, RNA; D014865:Waste Disposal, Fluid
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D012337:RNA, Ribosomal, 18S; D012338:RNA, Ribosomal, 23S
keywords: 18S–23S rRNA; Biodiesel; Lipids; Microalgae; Nisargruna biogas effluent