title: Akabane virus utilizes alternative endocytic pathways to entry into mammalian cell lines.
abstract: The entry mechanisms of Akabane virus (AKAV), Bunyaviridae family, have not yet been determined. In this study, chemical inhibitors were used to analyze endocytic mechanisms during AKAV infection of mammalian cell lines. The analyses using drug treatments followed by quantitative measurement of viral RNA and N protein revealed that AKAV enters non-bovine-derived cell lines (Vero, HmLu-1 and BHK cells) in a manner indicative of clathrin endocytosis. By contrast, AKAV infection in bovine-derived cell lines (LB9.K and MDBK cells) is independent of this pathway. Further analyses indicated that AKAV entry into bovine cell lines involves a non-clathrin, non-caveolae endocytic pathway that is dependent on dynamin. We conclude that although both cell types require a low pH for AKAV penetration, AKAV utilizes alternative entry pathways into mammalian cell lines.
authors: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors: Norasuthi
authors: N
authors: Bangphoomi
authors: Akiko
authors: A
authors: Takenaka-Uema
authors: Tatsuki
authors: T
authors: Sugi
authors: Kentaro
authors: K
authors: Kato
authors: Hiroomi
authors: H
authors: Akashi
authors: Taisuke
authors: T
authors: Horimoto
mesh_terms: D000818:Animals; D002043:Bunyaviridae / Q000502:physiology*; D002417:Cattle; D002522:Chlorocebus aethiops; D002772:Cholera Toxin / Q000378:metabolism; D006224:Cricetinae; D004705:Endocytosis / Q000502:physiology*; D008856:Microscopy, Fluorescence; D019590:Nucleocapsid Proteins / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D014168:Transferrin / Q000378:metabolism; D014709:Vero Cells; D053586:Virus Internalization
chemical_list: D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D014168:Transferrin; C115040:nucleocapsid protein, Akabane virus; D002772:Cholera Toxin