title: Analytical method for biomonitoring of perfluoroalkyl acids in human urine.
abstract: Perfluoroalkyl acids are an important class of synthetic compounds widely used in commercial and residential settings, which may have potential adverse health effects. The objective of this study was to monitor 6 perfluorocarboxylic acids and perfluorooctane sulphonate in human urine to obtain a way to asses exposure. The target analytes were extracted from urine by using a semi automated solid-phase extraction module and derivatised with isobutyl chloroformate by catalysis with 3% N,N-dicyclohexylcarbodiimide in pyridine. Determination and quantisation were achieved by gas chromatography with a mass spectrometer detector operating in the selected-ion monitoring mode. The developed approach is fast and provided low limits of detection (0.2-1.0 ng L(-1)) with good precision (relative standard deviation lower than 7.5%, within-day and between day). Recoveries from urine samples, which were spiked with the studied compounds at levels of 10 and 50 ng L(-1) ranged from 93% to 96%. Perfluorohexanoic (≤ 70 ng L(-1)) and perfluoroheptanoic acids (<2 ng L(-1)) were found in the urine samples from exposed researchers taken after handling these compounds. From the calculation of the excretion kinetics it was found that the dosage absorbed was eliminated within 15 h after exposure.
authors: Department of Analytical Chemistry, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain. Electronic address: bjsanchez@ucsd.edu.
authors: Beatriz
authors: B
authors: Jurado-Sánchez
authors: Department of Physical and Analytical Chemistry, E.P.S. of Linares, University of Jaén, E-23700 Linares, Jaén, Spain.
authors: Evaristo
authors: E
authors: Ballesteros
authors: Department of Analytical Chemistry, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain.
authors: Mercedes
authors: M
authors: Gallego
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D004784:Environmental Monitoring / Q000379:methods*; D005466:Fluorocarbons / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D005561:Formates / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007700:Kinetics; D016273:Occupational Exposure / Q000032:analysis; D015203:Reproducibility of Results; D012108:Research Personnel; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D005561:Formates; C039311:isobutyl chloroformate; C076994:perfluorooctane sulfonic acid
keywords: Derivatisation; Gas chromatography–mass spectrometry; Perfluorocarboxylic acids; Perfluorooctane sulphonate; Solid-phase extraction; Urine