title: Redundancy of primary RNA-binding functions of the bacterial transcription terminator Rho.
abstract: The bacterial transcription terminator, Rho, terminates transcription at half of the operons. According to the classical model derived from in vitro assays on a few terminators, Rho is recruited to the transcription elongation complex (EC) by recognizing specific sites (rut) on the nascent RNA. Here, we explored the mode of in vivo recruitment process of Rho. We show that sequence specific recognition of the rut site, in majority of the Rho-dependent terminators, can be compromised to a great extent without seriously affecting the genome-wide termination function as well as the viability of Escherichia coli. These terminators function optimally only through a NusG-assisted recruitment and activation of Rho. Our data also indicate that at these terminators, Rho-EC-bound NusG interaction facilitates the isomerization of Rho into a translocase-competent form by stabilizing the interactions of mRNA with the secondary RNA binding site, thereby overcoming the defects of the primary RNA binding functions.
authors: Laboratory of Transcription, Center for DNA Fingerprinting and Diagnostics, Tuljaguda Complex, 4-1-714 Mozamjahi Road, Nampally, Hyderabad 500001, India.
authors: Rajesh
authors: R
authors: Shashni
authors: Laboratory of Transcription, Center for DNA Fingerprinting and Diagnostics, Tuljaguda Complex, 4-1-714 Mozamjahi Road, Nampally, Hyderabad 500001, India.
authors: M Zuhaib
authors: MZ
authors: Qayyum
authors: Laboratory of Transcription, Center for DNA Fingerprinting and Diagnostics, Tuljaguda Complex, 4-1-714 Mozamjahi Road, Nampally, Hyderabad 500001, India.
authors: V
authors: V
authors: Vishalini
authors: Laboratory of Transcription, Center for DNA Fingerprinting and Diagnostics, Tuljaguda Complex, 4-1-714 Mozamjahi Road, Nampally, Hyderabad 500001, India.
authors: Debashish
authors: D
authors: Dey
authors: Laboratory of Transcription, Center for DNA Fingerprinting and Diagnostics, Tuljaguda Complex, 4-1-714 Mozamjahi Road, Nampally, Hyderabad 500001, India rsen@cdfd.org.in.
authors: Ranjan
authors: R
authors: Sen
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001665:Binding Sites; D029968:Escherichia coli Proteins / Q000235:genetics; D009154:Mutation; D010445:Peptide Elongation Factors / Q000235:genetics; D039002:Prophages / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012234:Rho Factor / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics; D061806:Transcription Termination, Genetic; D059467:Transcriptome
chemical_list: D029968:Escherichia coli Proteins; C070291:NusG protein, E coli; D010445:Peptide Elongation Factors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012234:Rho Factor; D014157:Transcription Factors; D000255:Adenosine Triphosphate