title: Microarray immobilization of biomolecules using a fast trans-cyclooctene (TCO)-tetrazine reaction.
abstract: By anchoring 1,2,4,5-tetrazine-containing biomolecules onto trans-cyclooctene (TCO)-functionalized slides, a site-specific microarray immobilization approach is described in this study. Compared with existing immobilization methods, our approach offers several distinctive features, including fast kinetics and high chemoselectivity.
authors: Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, P. R. China. hongysun@cityu.edu.hk.
authors: Ping
authors: P
authors: Wang
authors: Zhenkun
authors: Z
authors: Na
authors: Jiaqi
authors: J
authors: Fu
authors: Chelsea Y J
authors: CY
authors: Tan
authors: Huatang
authors: H
authors: Zhang
authors: Shao Q
authors: SQ
authors: Yao
authors: Hongyan
authors: H
authors: Sun
mesh_terms: D000595:Amino Acid Sequence; D034242:Cyclooctanes / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D034242:Cyclooctanes; D055767:Immobilized Proteins; D010455:Peptides; D011506:Proteins