title: [Transfusion of necrotic cells redresses regulatory T cell and Th17 cell imbalance in septic mice].
abstract: OBJECTIVE
  To investigate the effect of transfusion of necrotic cells on regulatory T (Treg) and Th17 cell balance in septic mice.
  
  
  METHODS
  Thirty-four C57BL/6 mice were randomized into PBS group (n=5), sham-operated group (n=5), sepsis group (n=12), and necrotic cell transfusion group (n=12) and subjected to intraperitoneal PBS injection, sham operation by separating the cecum only, cecal ligation and puncture (CLP), and injection of 2 × 10⁷ necrotic cells 5 days before CLP, respectively. All the mice were sacrificed 2 weeks after CLP for analyzing the proportion of CD4⁺Foxp3⁺Treg cells and CD4⁺IL17A⁺Th17 cells in the peripheral blood, spleen and thymus by flow cytometry.
  
  
  RESULTS
  The percentage of Th17 cells and Treg/Th17 ratio in the spleen was significantly higher in CLP group than in the sham-operated group and PBS group (P<0.01). The percentage of Treg cells in the thymus was significantly lower in CLP group than in the sham-operated group (P<0.01). Pre-infusion of necrotic cells redressed the abnormality of Treg and Th17 cell percentages and Treg/Th17 imbalance in mice following CLP (P<0.05).
  
  
  CONCLUSIONS
  Pre-infusion of necrotic cells can reverse Treg/Th17 imbalance in septic mice.
authors: Department of Rheumatism and Immunity, Third Affiliated Hospital, Southern Medical University, Guangzhou 510630, Chin. E-mail: rmjy1981@163.com.
authors: Dongsheng
authors: D
authors: Gu
authors: Lu
authors: L
authors: Xiong
authors: Yanping
authors: Y
authors: Han
authors: Erwei
authors: E
authors: Sun
mesh_terms: D000818:Animals; D002432:Cecum; D005434:Flow Cytometry; D020381:Interleukin-17; D008026:Ligation; D051379:Mice; D008810:Mice, Inbred C57BL; D009336:Necrosis; D018805:Sepsis / Q000628:therapy*; D013154:Spleen; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*; D058504:Th17 Cells / Q000166:cytology*; D013950:Thymus Gland
chemical_list: D020381:Interleukin-17