title: Periaortic brown adipose tissue as a major determinant of [¹⁸F]-fluorodeoxyglucose vascular uptake in atherosclerosis-prone, apoE-/- mice.
abstract: BACKGROUND
  [18F]-fluorodeoxyglucose (FDG) has been suggested for the clinical and experimental imaging of inflammatory atherosclerotic lesions. Significant FDG uptake in brown adipose tissue (BAT) has been observed both in humans and mice. The objective of the present study was to investigate the influence of periaortic BAT on apolipoprotein E-deficient (apoE-/-) mouse atherosclerotic lesion imaging with FDG.
  
  
  METHODS
  ApoE-/- mice (36 ± 2 weeks-old) were injected with FDG (12 ± 2 MBq). Control animals (Group A, n = 7) were injected conscious and kept awake at room temperature (24°C) throughout the accumulation period. In order to minimize tracer activity in periaortic BAT, Group B (n = 7) and C (n = 6) animals were injected under anaesthesia at 37°C and Group C animals were additionally pre-treated with propranolol. PET/CT acquisitions were performed prior to animal euthanasia and ex vivo analysis of FDG biodistribution.
  
  
  RESULTS
  Autoradiographic imaging indicated higher FDG uptake in atherosclerotic lesions than in the normal aortic wall (all groups, P<0.05) and the blood (all groups, P<0.01) which correlated with macrophage infiltration (R = 0.47; P<0.001). However, periaortic BAT uptake was either significantly higher (Group A, P<0.05) or similar (Group B and C, P = NS) to that observed in atherosclerotic lesions and was shown to correlate with in vivo quantified aortic FDG activity.
  
  
  CONCLUSIONS
  Periaortic BAT FDG uptake was identified as a confounding factor while using FDG for the non-invasive imaging of mouse atherosclerotic lesions.
authors: INSERM, UMR 1039, Radiopharmaceutiques Biocliniques; Université Grenoble I, La Tronche, France.
authors: Jakub
authors: J
authors: Toczek
authors: INSERM, UMR 1039, Radiopharmaceutiques Biocliniques; Université Grenoble I, La Tronche, France.
authors: Alexis
authors: A
authors: Broisat
authors: INSERM, UMR 1039, Radiopharmaceutiques Biocliniques; Université Grenoble I, La Tronche, France.
authors: Pascale
authors: P
authors: Perret
authors: INSERM, UMR 1039, Radiopharmaceutiques Biocliniques; Université Grenoble I, La Tronche, France.
authors: Marie-Dominique
authors: MD
authors: Desruet
authors: INSERM, UMR 1039, Radiopharmaceutiques Biocliniques; Université Grenoble I, La Tronche, France.
authors: Daniel
authors: D
authors: Fagret
authors: INSERM, UMR 1039, Radiopharmaceutiques Biocliniques; Université Grenoble I, La Tronche, France.
authors: Laurent M
authors: LM
authors: Riou
authors: INSERM, UMR 1039, Radiopharmaceutiques Biocliniques; Université Grenoble I, La Tronche, France.
authors: Catherine
authors: C
authors: Ghezzi
mesh_terms: D002001:Adipose Tissue, Brown / Q000000981:diagnostic imaging*; D000818:Animals; D001011:Aorta / Q000000981:diagnostic imaging*; D001057:Apolipoproteins E / Q000235:genetics*; D050197:Atherosclerosis / Q000000981:diagnostic imaging* / Q000235:genetics; D001835:Body Weight; D005260:Female; D019788:Fluorodeoxyglucose F18; D051379:Mice; D018345:Mice, Knockout; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D014018:Tissue Distribution
chemical_list: D001057:Apolipoproteins E; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18