title: Neural crest stem cells from hair follicles and boundary cap have different effects on pancreatic islets in vitro.
abstract: OBJECTIVE
  Neural crest stem cells derived from the boundary cap (bNCSCs), markedly promote survival, proliferation and function of insulin producing β-cells in vitro and in vivo after coculture/transplantation with pancreatic islets [ 1, 2 ]. Recently, we have shown that beneficial effects on β-cells require cadherin contacts between bNCSCs and β-cells [ 3, 4 ]. Here we investigated whether hair follicle (HF) NCSCs, a potential source for human allogeneic transplantation, exert similar positive effects on β-cells.
  
  
  METHODS
  We established cocultures of HF-NCSCs or bNCSCs from mice expressing enhanced green fluorescent protein together with pancreatic islets from DxRed expressing mice or NMRI mice and compared their migration towards islet cells and effect on proliferation of β-cells as well as intracellular relations between NCSCs and islets using qRT-PCR analysis and immunohistochemistry.
  
  
  RESULTS
  Whereas both types of NCSCs migrated extensively in the presence of islets, only bNCSCs demonstrated directed migration toward islets, induced β-cell proliferation and increased the presence of cadherin at the junctions between bNCSCs and β-cells. Even in direct contact between β-cells and HF-NCSCs, no cadherin expression was detected.
  
  
  CONCLUSIONS
  These observations indicate that HF-NCSCs do not confer the same positive effect on β-cells as demonstrated for bNCSCs. Furthermore, these data suggest that induction of cadherin expression by HF-NCSCs may be useful for their ability to support β-cells in coculture and after transplantation.
authors: 1Department of Neuroscience, Uppsala University Biomedical Center, Uppsala, Sweden.
authors: Anastasiia
authors: A
authors: Kosykh
authors: Anongnad
authors: A
authors: Ngamjariyawat
authors: Svitlana
authors: S
authors: Vasylovska
authors: Niclas
authors: N
authors: Konig
authors: Carl
authors: C
authors: Trolle
authors: Joey
authors: J
authors: Lau
authors: Arsen
authors: A
authors: Mikaelyan
authors: Michael
authors: M
authors: Panchenko
authors: Per-Ola
authors: PO
authors: Carlsson
authors: Ekaterina
authors: E
authors: Vorotelyak
authors: Elena N
authors: EN
authors: Kozlova
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D004622:Embryo, Mammalian; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology*; D066298:In Vitro Techniques; D050417:Insulin-Secreting Cells / Q000502:physiology; D007515:Islets of Langerhans / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009432:Neural Crest / Q000166:cytology*; D058953:Neural Stem Cells / Q000502:physiology* / Q000637:transplantation*; D013997:Time Factors
chemical_list: D000199:Actins; D015820:Cadherins; D049452:Green Fluorescent Proteins
keywords: Diabetes; cell culture; coculture; intercellular contacts; migration