title: Expression of Bcl-2 and NF-κB in brain tissue after acute renal ischemia-reperfusion in rats.
abstract: OBJECTIVE
  To investigate the effect of acute renal ischemia reperfusion on brain tissue.
  
  
  METHODS
  Fourty eight rats were randomly divided into four groups (n=12): sham operation group, 30 min ischemia 60 min reperfusion group, 60 min ischemia 60 min reperfusion group, and 120 min ischemia 60 min reperfusion group. The brain tissues were taken after the experiment. TUNEL assay was used to detect the brain cell apoptosis, and western blot was used to detect the expression of apoptosis-related proteins and inflammatory factors.
  
  
  RESULTS
  Renal ischemia-reperfusion induced apoptosis of brain tissues, and the apoptosis increased with prolongation of ischemia time. The detection at the molecular level showed decreased Bcl-2 expression, increased Bax expression, upregulated expression of NF-κB and its downstream factor COX-2/PGE2.
  
  
  CONCLUSIONS
  Acute renal ischemia-reperfusion can cause brain tissue damage, manifested as induced brain tissues apoptosis and inflammation activation.
authors: Department of Neurosurgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors: Na
authors: N
authors: Zhang
authors: Department of Nephrology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: chengrooter@163.com.
authors: Gen-Yang
authors: GY
authors: Cheng
authors: Department of Neurosurgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors: Xian-Zhi
authors: XZ
authors: Liu
authors: Department of Neurosurgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors: Feng-Jiang
authors: FJ
authors: Zhang
mesh_terms: D058186:Acute Kidney Injury / Q000378:metabolism*; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000032:analysis / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D001923:Brain Chemistry; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D016207:Cytokines; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: Acute renal ischemia reperfusion; Bcl-2; Brain tissue damage; NF-κB