title: SELDI-TOF-MS proteomic profiling of serum, urine, and amniotic fluid in neural tube defects.
abstract: Neural tube defects (NTDs) are common birth defects, whose specific biomarkers are needed. The purpose of this pilot study is to determine whether protein profiling in NTD-mothers differ from normal controls using SELDI-TOF-MS. ProteinChip Biomarker System was used to evaluate 82 maternal serum samples, 78 urine samples and 76 amniotic fluid samples. The validity of classification tree was then challenged with a blind test set including another 20 NTD-mothers and 18 controls in serum samples, and another 19 NTD-mothers and 17 controls in urine samples, and another 20 NTD-mothers and 17 controls in amniotic fluid samples. Eight proteins detected in serum samples were up-regulated and four proteins were down-regulated in the NTD group. Four proteins detected in urine samples were up-regulated and one protein was down-regulated in the NTD group. Six proteins detected in amniotic fluid samples were up-regulated and one protein was down-regulated in the NTD group. The classification tree for serum samples separated NTDs from healthy individuals, achieving a sensitivity of 91% and a specificity of 97% in the training set, and achieving a sensitivity of 90% and a specificity of 97% and a positive predictive value of 95% in the test set. The classification tree for urine samples separated NTDs from controls, achieving a sensitivity of 95% and a specificity of 94% in the training set, and achieving a sensitivity of 89% and a specificity of 82% and a positive predictive value of 85% in the test set. The classification tree for amniotic fluid samples separated NTDs from controls, achieving a sensitivity of 93% and a specificity of 89% in the training set, and achieving a sensitivity of 90% and a specificity of 88% and a positive predictive value of 90% in the test set. These suggest that SELDI-TOF-MS is an additional method for NTDs pregnancies detection.
authors: Department of Pediatric Surgery, The Shengjing Hospital, China Medical University, Heping District, Shenyang City, Liaoning Province, People's Republic of China.
authors: Zhenjiang
authors: Z
authors: Liu
authors: Department of Pediatric Surgery, The Shengjing Hospital, China Medical University, Heping District, Shenyang City, Liaoning Province, People's Republic of China.
authors: Zhengwei
authors: Z
authors: Yuan
authors: Department of Pediatric Surgery, The Shengjing Hospital, China Medical University, Heping District, Shenyang City, Liaoning Province, People's Republic of China.
authors: Qun
authors: Q
authors: Zhao
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry*; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D033261:Maternal-Fetal Relations; D009436:Neural Tube Defects / Q000097:blood* / Q000652:urine*; D010865:Pilot Projects; D020543:Proteome / Q000032:analysis*
chemical_list: D001798:Blood Proteins; D020543:Proteome