title: Genomic characterization of coxsackievirus type A24 strains associated with acute flaccid paralysis and rarely identified Hopkins syndrome.
abstract: The full-length genome sequence analysis of four coxsackievirus A24 (CV-A24) strains, detected in three paralytic and one post-asthmatic paralytic (Hopkins syndrome) cases, is reported here for the first time. A phylogenetic tree constructed on the basis of entire genomes displayed topology similar to that of the full-VP1 tree, classifying the study strains in genogroup CV-A24vGIV along with their temporal counterparts in strains from non-paralytic cases. The strains of the study formed a single genetic cluster C4 within CV-A24vGIV and showed 3.5-19.4 % nucleotide sequence divergence, with 2-4 novel nucleotide mutations in the 5'NCR and 3-8 unique amino acid substitutions in the polyprotein, with respect to the CV-A24 strains associated with non-paralytic cases. Among the nucleotide mutations, A299U was identified in the 5'NCRs of all of the study strains. CV-A24v strains of the same genogroup with few genomic variations but different disease manifestations need to be explored to investigate the molecular basis of evolution of neurovirulence.
authors: Enteric Viruses Group, National Institute of Virology, 20-A, Dr Ambedkar Road, Pune, 411 001, Maharashtra, India.
authors: Rongala
authors: R
authors: Laxmivandana
authors: Prasanna
authors: P
authors: Yergolkar
authors: Mannapur
authors: M
authors: Rajeshwari
authors: Shobha D
authors: SD
authors: Chitambar
mesh_terms: D003384:Coxsackievirus Infections / Q000821:virology*; D030015:Enterovirus C, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D016679:Genome, Viral; D023281:Genomics; D006801:Humans; D008969:Molecular Sequence Data; D010243:Paralysis / Q000821:virology*; D010802:Phylogeny