title: [Clustered regularly interspaced short palindromic repeat associated protein genes cas1 and cas2 in Shigella].
abstract: OBJECTIVE
  To detect the distribution of clustered regularly interspaced short palindromic repeat (CRISPR) associated protein genes cas1 and cas2 in Shigella and to understand the characteristics of CRISPR with relationship between CRISPR and related characteristics on drug resistance.
  
  
  METHODS
  CRISPR associated protein genes cas1 and cas2 in Shigella were detected by PCR, with its products sequenced and compared.
  
  
  RESULTS
  The CRISPR-associated protein genes cas1 and cas2 were found in all the 196 Shigella isolates which were isolated at different times and locations in China. Consistencies showed through related sequencing appeared as follows: cas2, cas1 (a) and cas1 (b) were 96.44%, 97.61% and 96.97%, respectively. There were two mutations including 3177129 site(C→G)and 3177126 site (G→C) of cas1 (b) gene in 2003135 strain which were not found in the corresponding sites of Z23 and 2008113.
  
  
  RESULTS
  showed that in terms of both susceptibility and antibiotic-resistance, strain 2003135 was stronger than Z23 and 2008113.
  
  
  CONCLUSIONS
  CRISPR system widely existed in Shigella, with the level of drug resistance in cas1 (b) gene mutant strains higher than in wild strains. Cas1 (b) gene mutation might be one of the reasons causing the different levels of resistance.
authors: School of Public Health, Zhengzhou Universtiy, Zhengzhou 450001, China.
authors: Zerun
authors: Z
authors: Xue
authors: School of Public Health, Zhengzhou Universtiy, Zhengzhou 450001, China; Henan University of Science and Technology.
authors: Yingfang
authors: Y
authors: Wang
authors: School of Public Health, Zhengzhou Universtiy, Zhengzhou 450001, China. Email: gcduan@zzu.edu.cn.
authors: Guangcai
authors: G
authors: Duan
authors: School of Public Health, Zhengzhou Universtiy, Zhengzhou 450001, China.
authors: Pengfei
authors: P
authors: Wang
authors: School of Public Health, Zhengzhou Universtiy, Zhengzhou 450001, China.
authors: Linlin
authors: L
authors: Wang
authors: School of Public Health, Zhengzhou Universtiy, Zhengzhou 450001, China.
authors: Xiangjiao
authors: X
authors: Guo
authors: School of Public Health, Zhengzhou Universtiy, Zhengzhou 450001, China.
authors: Yuanlin
authors: Y
authors: Xi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D064130:CRISPR-Associated Proteins / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D024881:Drug Resistance, Bacterial / Q000235:genetics; D009154:Mutation; D012760:Shigella / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D064130:CRISPR-Associated Proteins