title: Multi-modal label-free imaging based on a femtosecond fiber laser.
abstract: We demonstrate multi-mode microscopy based on a single femtosecond fiber laser. Coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and photothermal images can be obtained simultaneously with this simplified setup. Distributions of lipid and hemoglobin in sliced mouse brain samples and blood cells are imaged. The dependency of signal amplitude on the pump power and pump modulation frequency is characterized, which allows to isolate the impact from different contributions.
authors: Department Electrical Engineering & Computer Science, University of Kansas, Lawrence, KS, 66045, USA.
authors: Ruxin
authors: R
authors: Xie
authors: Department Electrical Engineering & Computer Science, University of Kansas, Lawrence, KS, 66045, USA.
authors: Jue
authors: J
authors: Su
authors: Department of Chemistry, University of Kansas, Lawrence, KS, 66045, USA.
authors: Eric C
authors: EC
authors: Rentchler
authors: Department of Pharmacology & Toxicology, University of Kansas, Lawrence, KS, 66045, USA.
authors: Ziyan
authors: Z
authors: Zhang
authors: Department of Chemistry, University of Kansas, Lawrence, KS, 66045, USA.
authors: Carey K
authors: CK
authors: Johnson
authors: Department of Pharmacology & Toxicology, University of Kansas, Lawrence, KS, 66045, USA.
authors: Honglian
authors: H
authors: Shi
authors: Department Electrical Engineering & Computer Science, University of Kansas, Lawrence, KS, 66045, USA.
authors: Rongqing
authors: R
authors: Hui
keywords: (060.5295) Photonic crystal fibers; (140.3510) Lasers, fiber; (170.3880) Medical and biological imaging; (180.4315) Nonlinear microscopy; (300.6230) Spectroscopy, coherent anti-Stokes Raman scattering; (300.6430) Spectroscopy, photothermal; (300.6450) Spectroscopy, Raman