title: Effects of Methylcellulose on Cellulolytic Bacteria Attachment and Rice Straw Degradation in the In vitro Rumen Fermentation.
abstract: An in vitro experiment was conducted to evaluate the effect of methylcellulose on the attachment of major cellulolytic bacteria on rice straw and its digestibility. Rice straw was incubated with ruminal mixture with or without 0.1% methylcellulose (MC). The attachment of F. succinogenes, R. flavefaciens and R. albus populations on rice straw was measured using real-time PCR with specific primer sets. Methylcellulose at the level of 0.1% decreased the attachment of all three major cellulolytic bacteria. In particular, MC treatment reduced (p<0.05) attachment of F. succinogenes on rice straw after 10 min of incubation while a significant reduction (p<0.05) in attachment was not observed until 4 h incubation in the case of R. flavefaciens and R. albus. This result indicated F. succinogenes responded to MC more sensitively and earlier than R. flavefaciens and R. albus. Dry matter digestibility of rice straw was subsequently inhibited by 0.1% MC, and there was a significant difference between control and MC treatment (p<0.05). Incubated cultures containing MC had higher pH and lower gas production than controls. Current data clearly indicated that the attachment of F. succinogenes, R. flavefaciens and R. albus on rice straw was inhibited by MC, which apparently reduced rice straw digestion.
authors: Department of Animal Science and Technology, Sangji University, Wonju, 220-702, Korea.
authors: Ha Guyn
authors: HG
authors: Sung
authors: Department of Animal Science and Technology, Sangji University, Wonju, 220-702, Korea.
authors: Min Ji
authors: MJ
authors: Kim
authors: Department of Animal Science and Technology, Sangji University, Wonju, 220-702, Korea.
authors: Santi Devi
authors: SD
authors: Upadhaya
authors: Department of Animal Science and Technology, Sangji University, Wonju, 220-702, Korea.
authors: Jong K
authors: JK
authors: Ha
authors: Department of Animal Science and Technology, Sangji University, Wonju, 220-702, Korea.
authors: Sung Sill
authors: SS
authors: Lee
keywords: Bacterial Attachment; Cellulolytic Bacteria; Cellulose Digestion; Methylcellulose; Rice Straw