title: The Mutagenesis Assistant Program.
abstract: Mutagenesis Assistant Program (MAP) is a web-based statistical tool to develop directed evolution strategies by investigating the consequences at the amino acid level of the mutational biases of random mutagenesis methods on any given gene. The latest development of the program, the MAP(2.0)3D server, correlates the generated amino acid substitution patterns of a specific random mutagenesis method to the sequence and structural information of the target protein. The combined information can be used to select an experimental strategy that improves the chances of obtaining functionally efficient and/or stable enzyme variants. Hence, the MAP(2.0)3D server facilitates the "in silico" prescreening of the target gene by predicting the amino acid diversity generated in a random mutagenesis library. Here, we describe the features of MAP(2.0)3D server by analyzing, as an example, the cytochrome P450BM3 monooxygenase (CYP102A1). The MAP(2.0)3D server is available publicly at http://map.jacobs-university.de/map3d.html.
authors: Department of Chemistry, Wichita State University, Wichita, Kansas, USA.
authors: Rajni
authors: R
authors: Verma
authors: Tuck Seng
authors: TS
authors: Wong
authors: Ulrich
authors: U
authors: Schwaneberg
authors: Danilo
authors: D
authors: Roccatano
mesh_terms: D000465:Algorithms; D019020:Directed Molecular Evolution; D020407:Internet; D012984:Software