title: Modeling the growth of Listeria monocytogenes on the surface of smear- or mold-ripened cheese.
abstract: Surface-ripened cheeses are matured by means of manual or mechanical technologies posing a risk of cross-contamination, if any cheeses are contaminated with Listeria monocytogenes. In predictive microbiology, primary models are used to describe microbial responses, such as growth rate over time and secondary models explain how those responses change with environmental factors. In this way, primary models were used to assess the growth rate of L. monocytogenes during ripening of the cheeses and the secondary models to test how much the growth rate was affected by either the pH and/or the water activity (aw) of the cheeses. The two models combined can be used to predict outcomes. The purpose of these experiments was to test three primary (the modified Gompertz equation, the Baranyi and Roberts model, and the Logistic model) and three secondary (the Cardinal model, the Ratowski model, and the Presser model) mathematical models in order to define which combination of models would best predict the growth of L. monocytogenes on the surface of artificially contaminated surface-ripened cheeses. Growth on the surface of the cheese was assessed and modeled. The primary models were firstly fitted to the data and the effects of pH and aw on the growth rate (μmax) were incorporated and assessed one by one with the secondary models. The Logistic primary model by itself did not show a better fit of the data among the other primary models tested, but the inclusion of the Cardinal secondary model improved the final fit. The aw was not related to the growth of Listeria. This study suggests that surface-ripened cheese should be separately regulated within EU microbiological food legislation and results expressed as counts per surface area rather than per gram.
authors: Food Safety Department, Teagasc Food Research Centre Moorepark, Fermoy, Ireland ; Biosystems Engineering, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin Dublin, Ireland.
authors: M Sol
authors: MS
authors: Schvartzman
authors: Biosystems Engineering, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin Dublin, Ireland.
authors: Ursula
authors: U
authors: Gonzalez-Barron
authors: Biosystems Engineering, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin Dublin, Ireland.
authors: Francis
authors: F
authors: Butler
authors: Food Safety Department, Teagasc Food Research Centre Moorepark, Fermoy, Ireland.
authors: Kieran
authors: K
authors: Jordan
mesh_terms: D000465:Algorithms; D002611:Cheese / Q000382:microbiology*; D005516:Food Microbiology; D005658:Fungi / Q000254:growth & development; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000254:growth & development*; D008954:Models, Biological; D015233:Models, Statistical; D015203:Reproducibility of Results; D013696:Temperature
keywords: Baranyi and Roberts; Gompertz; Listeria monocytogenes; Presser; growth; mathematical modeling