title: Fine mapping of the tomato yellow leaf curl virus resistance geneTy-2on chromosome 11 of tomato.
abstract: Resistances to begomoviruses, including bipartite tomato mottle virus and monopartite tomato yellow leaf curl virus (TYLCV), have been introgressed to cultivated tomato (Solanum lycopersicum) from wild tomato accessions. A major gene,Ty-2fromS. habrochaitesf.glabratumaccession "B6013,"that confers resistance to TYLCV was previously mapped to a 19-cM region on the long arm of chromosome 11. In the present study, approximately 11,000 plants were screened and nearly 157 recombination events were identified between the flanking markers C2_At1g07960 (82.5 cM, physical distance 51.387 Mb) and T0302 (89 cM, 51.878 Mb). Molecular marker analysis of recombinants and TYLCV evaluation of progeny from these recombinants localizedTy-2to an approximately 300,000-bp interval between markers UP8 (51.344 Mb) and M1 (51.645 Mb). No recombinants were identified between TG36 and C2_At3g52090, a region of at least 115 kb, indicating severe recombination suppression in this region. Due to the small interval, fluorescence in situ hybridization analysis failed to clarify whether recombination suppression is caused by chromosomal rearrangements. Candidate genes predicted based on tomato genome annotation were analyzed by RT-PCR and virus-induced gene silencing. Results indicate that the NBS gene family present in theTy-2region is likely not responsible for theTy-2-conferred resistance and that two candidate genes might play a role in theTy-2-conferred resistance. Several markers very tightly linked to theTy-2locus are presented and useful for marker-assisted selection in breeding programs to introgressTy-2for begomovirus resistance.
authors: Institute of Vegetable and Flowers, The Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Haidian District, Beijing, 100081 China ; Shandong Key Laboratory for Biology of Greenhouse Vegetables, Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Jinan, China ; Gulf Coast Research and Education Center, University of Florida, 14625 CR 672, Wimauma, FL 33598 USA.
authors: Xiaohui
authors: X
authors: Yang
authors: Wageningen UR Plant Breeding, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Myluska
authors: M
authors: Caro
authors: Gulf Coast Research and Education Center, University of Florida, 14625 CR 672, Wimauma, FL 33598 USA.
authors: Samuel F
authors: SF
authors: Hutton
authors: Gulf Coast Research and Education Center, University of Florida, 14625 CR 672, Wimauma, FL 33598 USA.
authors: John W
authors: JW
authors: Scott
authors: Institute of Vegetable and Flowers, The Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Haidian District, Beijing, 100081 China.
authors: Yanmei
authors: Y
authors: Guo
authors: Institute of Vegetable and Flowers, The Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Haidian District, Beijing, 100081 China.
authors: Xiaoxuan
authors: X
authors: Wang
authors: Wageningen UR Plant Breeding, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Md Harunur
authors: MH
authors: Rashid
authors: Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Dora
authors: D
authors: Szinay
authors: Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Hans
authors: H
authors: de Jong
authors: Wageningen UR Plant Breeding, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Richard G F
authors: RG
authors: Visser
authors: Wageningen UR Plant Breeding, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Yuling
authors: Y
authors: Bai
authors: Institute of Vegetable and Flowers, The Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Haidian District, Beijing, 100081 China.
authors: Yongchen
authors: Y
authors: Du
keywords: Breeding; Resistance; Tomato; Tomato yellow leaf curling virus (TYLCV); Virus-induced gene silencing