title: Engineering β-lactamase zymogens for use in protease activity assays.
abstract: In this study, the concept of autoinhibition, a mechanism of protein activity regulation, was applied to the design and engineering of a β-lactamase zymogen. Using this zymogen, a sensitive protease assay method was developed in which activation of the zymogen by proteases produces an amplified absorbance signal. The approach reported here can be adapted for engineering of zymogens as biological sensors and components of synthetic signaling pathways.
authors: Department of Molecular Science and Technology, Ajou University, 206 World cup-ro, Yeongtong-gu, Suwon 443-749, South Korea.
authors: Hajin
authors: H
authors: Kim
authors: Hyun Kyung
authors: HK
authors: Yoon
authors: Tae Hyeon
authors: TH
authors: Yoo
mesh_terms: D004789:Enzyme Activation / Q000502:physiology; D004792:Enzyme Precursors / Q000138:chemical synthesis* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D017433:Protein Structure, Secondary; D001618:beta-Lactamases / Q000138:chemical synthesis* / Q000378:metabolism
chemical_list: D004792:Enzyme Precursors; D010447:Peptide Hydrolases; D001618:beta-Lactamases