title: Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats.
abstract: MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats.
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No.61, Taian 271018, Shandong Province, China.
authors: Zhibin
authors: Z
authors: Ji
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No.61, Taian 271018, Shandong Province, China.
authors: Guizhi
authors: G
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No.61, Taian 271018, Shandong Province, China.
authors: Chunlan
authors: C
authors: Zhang
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No.61, Taian 271018, Shandong Province, China.
authors: Zhijing
authors: Z
authors: Xie
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No.61, Taian 271018, Shandong Province, China.
authors: Zhaohua
authors: Z
authors: Liu
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No.61, Taian 271018, Shandong Province, China.
authors: Jianmin
authors: J
authors: Wang
keywords: Goat; Mammary Gland; MicroRNA; Solexa High-throughput Sequencing