title: Identification of discrete classes of small nucleolar RNA featuring different ends and RNA binding protein dependency.
abstract: Small nucleolar RNAs (snoRNAs) are among the first discovered and most extensively studied group of small non-coding RNA. However, most studies focused on a small subset of snoRNAs that guide the modification of ribosomal RNA. In this study, we annotated the expression pattern of all box C/D snoRNAs in normal and cancer cell lines independent of their functions. The results indicate that C/D snoRNAs are expressed as two distinct forms differing in their ends with respect to boxes C and D and in their terminal stem length. Both forms are overexpressed in cancer cell lines but display a conserved end distribution. Surprisingly, the long forms are more dependent than the short forms on the expression of the core snoRNP protein NOP58, thought to be essential for C/D snoRNA production. In contrast, a subset of short forms are dependent on the splicing factor RBFOX2. Analysis of the potential secondary structure of both forms indicates that the k-turn motif required for binding of NOP58 is less stable in short forms which are thus less likely to mature into a canonical snoRNP. Taken together the data suggest that C/D snoRNAs are divided into at least two groups with distinct maturation and functional preferences.
authors: Département de biochimie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: Gabrielle
authors: G
authors: Deschamps-Francoeur
authors: Laboratoire de génomique fonctionnelle de l'Université de Sherbrooke, Québec J1E 4K8, Canada Département de microbiologie et d'infectiologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: Daniel
authors: D
authors: Garneau
authors: Département de biochimie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: Fabien
authors: F
authors: Dupuis-Sandoval
authors: Département de biochimie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: Audrey
authors: A
authors: Roy
authors: Département de biochimie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: Marie
authors: M
authors: Frappier
authors: Laboratoire de génomique fonctionnelle de l'Université de Sherbrooke, Québec J1E 4K8, Canada Département de microbiologie et d'infectiologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: Mathieu
authors: M
authors: Catala
authors: Laboratoire de génomique fonctionnelle de l'Université de Sherbrooke, Québec J1E 4K8, Canada Département de microbiologie et d'infectiologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: Sonia
authors: S
authors: Couture
authors: Département de biochimie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.
authors: Mélissa
authors: M
authors: Barbe-Marcoux
authors: Laboratoire de génomique fonctionnelle de l'Université de Sherbrooke, Québec J1E 4K8, Canada Département de microbiologie et d'infectiologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada michelle.scott@usherbrooke.ca.
authors: Sherif
authors: S
authors: Abou-Elela
authors: Département de biochimie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada michelle.scott@usherbrooke.ca.
authors: Michelle S
authors: MS
authors: Scott
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000502:physiology*; D009690:Nucleic Acid Conformation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism; D000072260:RNA Splicing Factors; D020537:RNA, Small Nucleolar / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000502:physiology*; D012097:Repressor Proteins / Q000502:physiology*; D020718:Ribonucleoproteins, Small Nucleolar / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: C499062:NOP58 protein, human; D009687:Nuclear Proteins; C454924:RBFOX2 protein, human; D000072260:RNA Splicing Factors; D020537:RNA, Small Nucleolar; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D020718:Ribonucleoproteins, Small Nucleolar