title: Amperometric magnetoimmunoassay for the direct detection of tumor necrosis factor alpha biomarker in human serum.
abstract: An amperometric immunoassay for the determination of tumor necrosis factor alpha (TNFα) protein biomarker in human serum based on the use of magnetic microbeads (MBs) and disposable screen-printed carbon electrodes (SPCEs) has been developed. The specifically modified microbeads were magnetically captured on the working electrode surface and the amperometric responses were measured at -0.20V (vs. Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as electron transfer mediator and H2O2 as the enzyme substrate. After a thorough optimization of the assay, extremely low limits of detection were achieved: 2.0pg mL(-1) (36fM) and 5.8pg mL(-1) (105fM) for standard solutions and spiked human serum, respectively. The simplicity, robustness and this clinically interesting LOD proved the developed TNFα immunoassay as a good contender for real clinical application.
authors: Micro-NanoFabrication Unit, IK4-Tekniker, Eibar, Spain; CIC microGUNE, Arrasate-Mondragón, Spain.
authors: U
authors: U
authors: Eletxigerra
authors: CIC microGUNE, Arrasate-Mondragón, Spain.
authors: J
authors: J
authors: Martinez-Perdiguero
authors: Micro-NanoFabrication Unit, IK4-Tekniker, Eibar, Spain; CIC microGUNE, Arrasate-Mondragón, Spain.
authors: S
authors: S
authors: Merino
authors: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors: R
authors: R
authors: Villalonga
authors: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain.
authors: J M
authors: JM
authors: Pingarrón
authors: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, Spain. Electronic address: susanacr@quim.ucm.es.
authors: S
authors: S
authors: Campuzano
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D055664:Electrochemical Techniques; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D048069:Tumor Necrosis Factors / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D048069:Tumor Necrosis Factors
keywords: Electrochemical; Human serum; Immunoassay; Magnetic beads; Tumor necrosis factor alpha