title: Green synthesis of Kocuran-functionalized silver glyconanoparticles for use as antibiofilm coatings on silicone urethral catheters.
abstract: Microbial infections due to biofilm formation on medical implants are serious complications arising after surgery which can be prevented by using antimicrobial coatings on biomaterial surfaces. We developed a simple, rapid and green chemistry approach for synthesis of silver glyconanoparticles (AgNPs) using Kocuran, an exopolysaccharide produced by Kocuria rosea strain BS-1. Kocuran-capped AgNPs exhibited a characteristic surface plasmon resonance (SPR) peak around 435 nm. They were mono-dispersed, spherical with an average particle size of 12 nm. XRD and SAED studies suggested that AgNPs were crystalline in nature. AgNPs had a zeta potential of -33.9 mV and were anionic charged. They showed colloidal stability at different pH (6 to 10), temperatures (30 °C to 100 °C), in NaCl, NaNO3 and BSA solutions. Kocuran-capped AgNPs exhibited effective antimicrobial activity against Staphylococcus aureus and Escherichia coli and cell death was mainly due to hydroxyl radical induction and depletion of NADH. They also inhibited the biofilm development by S. aureus and E. coli and confocal scanning laser microscopic images revealed the damage of intact cell architecture. In vitro evaluation of Kocuran-capped silver glyconanoparticles on human gingival fibroblasts demonstrated good cell proliferation as compared to commercial AgNPs suggesting that they are biocompatible and non-toxic in nature. This is a first report on Kocuran-functionalized AgNPs exhibiting potential antibacterial and antiadhesive properties for use as antimicrobial coatings against bacterial adhesion and biofilm formation on silicone urethral catheters.
authors: Academy of Scientific and Innovative Research, CSIR-Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500007, India. Medicinal Chemistry and Pharmacology Division, CSIR-Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500007, India.
authors: C Ganesh
authors: CG
authors: Kumar
authors: Pombala
authors: P
authors: Sujitha
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000138:chemical synthesis*; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D001688:Biological Products / Q000008:administration & dosage / Q000737:chemistry; D016923:Cell Death; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012828:Silicones; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D062885:Urinary Catheters
chemical_list: D000900:Anti-Bacterial Agents; D001688:Biological Products; C000591383:Kocuran; D010936:Plant Extracts; D011135:Polysaccharides, Bacterial; D012828:Silicones; D012834:Silver