title: Effects of MicroRNA-10b on lung cancer cell proliferation and invasive metastasis and the underlying mechanism.
abstract: OBJECTIVE
  To study the influence of MicroRNA-10b on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.
  
  
  METHODS
  Lipofectamine MicroRNA-10b eukaryotic expression plasmid was transfected into 95-C. The experiment group was divided into blank control group, empty vector transfected group and MicroRNA-10b transfected group. Real time quantitative RT-PCR was used to detect the expression of MicroRNA-10b and KLF4mRNA expression. Proliferations of cells were detected by cell proliferation assay, invasion of the detected the cell Transwell experiments, the expression of KLF4 protein was detected in Western blotting cells.
  
  
  RESULTS
  The proliferation rate of MicroRNA-10b plasmid transfection group increased significantly after transfection, invasion and migration ability enhancement, by comparison, there are statistically significant differences in the blank control group and negative control group (P<0.05); the expression of MicroRNA-10b plasmid transfection group KLF4 protein decreased, the difference was statistically significant (P<0.05); reduce the expression of MicroRNA-10b plasmid transfection group KLF4mRNA, but no significant differences (P>0.05).
  
  
  CONCLUSIONS
  MicroRNA-10b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.
authors: Department of Respiratory Medicine, Huaxi Hospital, Sichuan University, Chengdu, Sichuan 61004, China.
authors: Qiao-Li
authors: QL
authors: Su
authors: Department of Respiratory Medicine, Huaxi Hospital, Sichuan University, Chengdu, Sichuan 61004, China.
authors: Shuang-Qing
authors: SQ
authors: Li
authors: Department of Respiratory Medicine, Huaxi Hospital, Sichuan University, Chengdu, Sichuan 61004, China.
authors: Duo-Ning
authors: DN
authors: Wang
authors: Department of Respiratory Medicine, Huaxi Hospital, Sichuan University, Chengdu, Sichuan 61004, China.
authors: Feng
authors: F
authors: Liu
authors: Department of Respiratory Medicine, Huaxi Hospital, Sichuan University, Chengdu, Sichuan 61004, China.
authors: Bo
authors: B
authors: Yuan
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics*; D014162:Transfection
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C547183:MIRN10 microRNA, human; D035683:MicroRNAs
keywords: 95-C; Invasion; Lung cancer; MicroRNA-10b; Proliferation