title: Comparison of the serological tests ICT and ELISA for the diagnosis of alveolar echinococcosis in France.
abstract: Serological diagnosis of alveolar echinococcosis (AE) is a key element for efficient patient treatment management. A rapid immunochromatography test kit (ICT) using the recombinant Em18 antigen (rEm18) was recently developed. The aim of our study was to assess this test on a panel of sera from French patients with alveolar echinococcosis and control patients. In a blind test, a total of 112 serum samples were tested including samples of AE (n = 30), cystic echinococcosis [CE] (n = 15), and polycystic echinococcosis [PE] (n = 1). For the comparison, 66 sera from patients with hepatocarcinoma, fascioliasis, toxocariasis, Caroli's disease, or autoimmune chronic active hepatitis were used. The diagnostic test sets we used were the rEm18-ICT and two validated ELISAs with rEm18 and Em2-Em18 antigens, respectively. For the ICT, 27/30 sera from AE patients, 4/15 sera from CE patients and the PE patient serum were positive. One serum from the control panel (toxocariasis) was positive for the ICT. The rEm18-ICT sensitivity (90.0%) and specificity (92.7%) for detection of Em18-specific antibodies confirmed it as a relevant tool for AE diagnosis. The rEm18-ELISA had a sensitivity of 86.7% and specificity of 91.5%, and the Em2-Em18-ELISA had a sensitivity of 96.7% and specificity of 87.8%. However, when AE patient sera are recorded as weak in intensity with the ICT, we recommend a double reading and use of a reference sample if the ICT is used for patient follow-up.
authors: Laboratory of Chrono-environnement, UMR/CNRS 6249, Faculty of Medicine and Pharmacy, Besançon, France - WHO Collaborating Centre for prevention and treatment of human echinococcosis, Besançon, France.
authors: Jenny
authors: J
authors: Knapp
authors: Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan.
authors: Yasuhito
authors: Y
authors: Sako
authors: Laboratory of Chrono-environnement, UMR/CNRS 6249, Faculty of Medicine and Pharmacy, Besançon, France - WHO Collaborating Centre for prevention and treatment of human echinococcosis, Besançon, France - Laboratory of Parasitology-Mycology, University Hospital of Besançon, France.
authors: Frédéric
authors: F
authors: Grenouillet
authors: WHO Collaborating Centre for prevention and treatment of human echinococcosis, Besançon, France - Department of Hepatology, University Hospital of Besançon, France.
authors: Solange
authors: S
authors: Bresson-Hadni
authors: WHO Collaborating Centre for prevention and treatment of human echinococcosis, Besançon, France - Department of Hepatology, University Hospital of Besançon, France.
authors: Carine
authors: C
authors: Richou
authors: Laboratory of Chrono-environnement, UMR/CNRS 6249, Faculty of Medicine and Pharmacy, Besançon, France - Laboratory of Hospital Hygiene, University Hospital of Besançon, France.
authors: Houssein
authors: H
authors: Gbaguidi-Haore
authors: Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido, Japan.
authors: Akira
authors: A
authors: Ito
authors: Laboratory of Chrono-environnement, UMR/CNRS 6249, Faculty of Medicine and Pharmacy, Besançon, France - WHO Collaborating Centre for prevention and treatment of human echinococcosis, Besançon, France - Laboratory of Parasitology-Mycology, University Hospital of Besançon, France.
authors: Laurence
authors: L
authors: Millon
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D002846:Chromatography, Affinity; D003937:Diagnosis, Differential; D004444:Echinococcosis, Hepatic / Q000175:diagnosis* / Q000453:epidemiology; D048210:Echinococcus multilocularis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D005602:France / Q000453:epidemiology; D006801:Humans; D008107:Liver Diseases / Q000175:diagnosis; D009369:Neoplasms / Q000175:diagnosis; D010272:Parasitic Diseases / Q000175:diagnosis; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C117889:Em18 antigen; C000594589:Em2 antigen, Echinococcus multilocularis; D011994:Recombinant Proteins