title: Increased expression of GAP43 in interneurons in a rat model of experimental polymicrogyria.
abstract: To investigate seizure susceptibility in polymicrogyria, the seizure threshold and growth-associated protein GAP43 expression were analyzed in a rat experimental model of polymicrogyria induced by intracerebral injection of ibotenate. A total of 72 neonates from 9 pregnant rats were used. Intraperitoneal pentylenetetrazole injection did not induce any seizure activity in the control rats, although it elicited seizures of variable severity in the polymicrogyria rats. Fluoro-Jade B-positive degenerating interneurons were found in the polymicrogyria brains; however, no such neurons were detected in the control brains. In the polymicrogyria rats, the GAP43 expression was significantly and widely distributed in the brain, and the percentage of parvalbumin-positive interneurons in the GAP43-positive cells was significantly higher than that observed in the nonphosphorylated neurofilament-positive pyramidal cells. We conclude that the relatively selective vulnerability of inhibitory interneurons constitutes the basis for the decreased seizure threshold observed in this model of polymicrogyria.
authors: Department of Pediatrics, Shiga University of Medical Science, Seta-Tsukinowa, Otsu, Japan tmyktkn@belle.shiga-med.ac.jp.
authors: Tomoyuki
authors: T
authors: Takano
authors: Department of Pediatrics, Shiga University of Medical Science, Seta-Tsukinowa, Otsu, Japan.
authors: Katsuyuki
authors: K
authors: Matsui
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism*; D007051:Ibotenic Acid / Q000633:toxicity; D007395:Interneurons / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010320:Parvalbumins / Q000378:metabolism; D010433:Pentylenetetrazole / Q000633:toxicity; D065706:Polymicrogyria / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D011247:Pregnancy; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D012640:Seizures / Q000139:chemically induced
chemical_list: D019922:GAP-43 Protein; D010320:Parvalbumins; D007051:Ibotenic Acid; D010433:Pentylenetetrazole
keywords: GAP43; interneuron; polymicrogyria; seizure susceptibility