title: Integrated biomarkers in wild crucian carp for early warning of water quality in Hun River, North China.
abstract: Metabolizing enzymes play important roles in the detoxification of various pollutants in aquatic organisms, thereby they can also be used to provide early-warning signals of environmental risks. Real-time quantitative reverse-transcription polymerase chain reaction assays were developed to quantify cytochrome P450 1A (CYP1A), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and glutathione-S-transferase (GST) in crucian carp (Carassius auratus). The methods were then used to detect the respective mRNA expression levels in liver tissue in wild crucian carp from the Hun River, North China. CYP1A mRNA expression was significantly up-regulated in fish from stations S5, S6, and S8 (p < 0.05). SOD mRNA expression was significantly down-regulated in downstream areas relative to fish from upstream sites (p < 0.05); GPx and CAT mRNA expression levels were also down-regulated at S9 (p < 0.05). In contrast, GST mRNA expression showed no obvious change between fish collected from up- or downstream areas of the river. Finally, an integrated biomarker response was used to evaluate the integrated impact of pollutants in the Hun River and allow better comprehension of the real toxicological risk of these investigated sites.
authors: State Key Laboratory of Environmental Criteria and Risk Assessment & State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012. Electronic address: zhengbh@craes.org.cn.
authors: Binghui
authors: B
authors: Zheng
authors: State Key Laboratory of Environmental Criteria and Risk Assessment & State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012.
authors: Kun
authors: K
authors: Lei
authors: State Key Laboratory of Environmental Criteria and Risk Assessment & State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012.
authors: Ruizhi
authors: R
authors: Liu
authors: State Key Laboratory of Environmental Criteria and Risk Assessment & State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012.
authors: Shuangshuang
authors: S
authors: Song
authors: State Key Laboratory of Environmental Criteria and Risk Assessment & State Environmental Protection Key Laboratory of Estuarine and Coastal Research, Chinese Research Academy of Environment Sciences, Beijing 100012. Electronic address: anlhui@163.com.
authors: Lihui
authors: L
authors: An
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004784:Environmental Monitoring; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism*; D008969:Molecular Sequence Data; D013482:Superoxide Dismutase / Q000378:metabolism; D060753:Water Quality
chemical_list: D015415:Biomarkers; D002374:Catalase; D005979:Glutathione Peroxidase; D001189:Aryl Hydrocarbon Hydroxylases; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: Carassius auratus; Hun River; integrated biomarker response; real-time quantitative reverse-transcription polymerase chain reaction; water quality