title: Long non-coding RNA HOTAIR promotes carcinogenesis and invasion of gastric adenocarcinoma.
abstract: Gastric cancer is one of the major causes of cancer death worldwide; however, the mechanism of carcinogenesis is complex and poorly understood. Long non-coding RNA HOTAIR (HOX transcript antisense RNA) recently emerged as a promoter of metastasis in various cancers including gastric cancer. Here we investigated the impact of HOTAIR on apoptosis, cell proliferation and cell cycle to dissect the carcinogenesis of gastric cancer. We examined the mechanism of invasion and metastasis and analyzed the clinical significance of HOTAIR. Downregulation of HOTAIR was confirmed by two different siRNAs. The expression of HOTAIR was significantly elevated in various gastric cancer cell lines and tissues compared to normal control. si-HOTAIR significantly reduced viability in MKN 28, MKN 74, and KATO III cells but not in AGS cells. si-HOTAIR induced apoptosis in KATO III cells. Lymphovascular invasion and lymph node metastasis were more common in the high level of HOTAIR group. si-HOTAIR significantly decreased invasiveness and migration. si-HOTAIR led to differential expression of epithelial to mesenchymal transition markers. We found that HOTAIR was involved in inhibition of apoptosis and promoted invasiveness, supporting a role for HOTAIR in carcinogenesis and progression of gastric cancer.
authors: Division of Gastroenterology, Department of Internal Medicine, Yonsei Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors: Na Keum
authors: NK
authors: Lee
authors: Division of Gastroenterology, Department of Internal Medicine, Yonsei Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors: Jung Hwa
authors: JH
authors: Lee
authors: Division of Gastroenterology, Department of Internal Medicine, Yonsei Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors: Chan Hyuk
authors: CH
authors: Park
authors: Division of Gastroenterology, Department of Internal Medicine, Yonsei Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors: Dayeon
authors: D
authors: Yu
authors: Division of Gastroenterology, Department of Internal Medicine, Yonsei Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors: Yong Chan
authors: YC
authors: Lee
authors: Department of Surgery, Yonsei University College of Medicine, Republic of Korea.
authors: Jae-Ho
authors: JH
authors: Cheong
authors: Department of Surgery, Yonsei University College of Medicine, Republic of Korea.
authors: Sung Hoon
authors: SH
authors: Noh
authors: Division of Gastroenterology, Department of Internal Medicine, Yonsei Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Republic of Korea. Electronic address: sklee@yuhs.ac.
authors: Sang Kil
authors: SK
authors: Lee
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D017209:Apoptosis; D063646:Carcinogenesis / Q000235:genetics; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors / Q000235:genetics*; D012334:RNA, Neoplasm / Q000037:antagonists & inhibitors / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics* / Q000473:pathology
chemical_list: C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering
keywords: Apoptosis; Gastric cancer; HOTAIR; Invasion; Metastasis