title: Lectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against Haemonchus contortus.
abstract: Bioactive molecules of plant species are promising alternatives for the chemical control of gastrointestinal nematodes in ruminants. Extracts of native and exotic seed species from Brazil's semi-arid region were tested in vitro in an egg hatch assay and the bioactivity of their proteins was investigated. Each seed species was subjected to three extractions with three types of solvents. All the seeds showed ovicidal activity, which varied according to the solvents. Higher ovicidal activity was found in the molecule fractions of low molecular weight (<12 kDa) for Albizia lebbeck, Ipomoea asarifolia, Jatropha curcas, Libidibia ferrea, Moringa oleifera and Ricinus communis (P<0.05, Bonferroni test). The two fractions of Crotalaria spectabilis showed the same ovicidal activity (P>0.05, Bonferroni test). Hemagglutinating activity was detected in the fractions of C. spectabilis and M. oleifera fractions, hemolysin activity in the A. lebbeck and M. oleifera fractions, serine protease inhibitory activity in the A. lebbeck, I. asarifolia, J. curcas, M. oleifera and R. communis fractions, cysteine protease inhibitor activity in the M. oleifera fraction, and no protein activity in the L. ferrea fraction. The results of this work reveal new plant species with a potential for use in controlling nematode parasites in goats, thus opening a new field of research involving plant protein molecules with ovicidal properties.
authors: Embrapa Caprinos e Ovinos, Sobral, CE, Brasil.
authors: Hévila Oliveira
authors: HO
authors: Salles
authors: Instituto Superior de Teologia Aplicada ? INTA, Sobral, CE, Brasil.
authors: Ana Carolina Linhares
authors: AC
authors: Braga
authors: Universidade Estadual Vale do Acaraú ? UVA, Campus da Betânia, Sobral, CE, Brasil.
authors: Maria Thayana dos Santos Canuto do
authors: MT
authors: Nascimento
authors: Universidade Estadual Vale do Acaraú ? UVA, Campus da Betânia, Sobral, CE, Brasil.
authors: Ana Márjory Paiva
authors: AM
authors: Sousa
authors: Embrapa Caprinos e Ovinos, Sobral, CE, Brasil.
authors: Adriano Rodrigues
authors: AR
authors: Lima
authors: Embrapa Caprinos e Ovinos, Sobral, CE, Brasil.
authors: Luiz da Silva
authors: Lda S
authors: Vieira
authors: Embrapa Caprinos e Ovinos, Sobral, CE, Brasil.
authors: Antônio Cézar Rocha
authors: AC
authors: Cavalcante
authors: Embrapa Caprinos e Ovinos, Sobral, CE, Brasil.
authors: Antonio Silvio do
authors: AS
authors: Egito
authors: Universidade Estadual Vale do Acaraú ? UVA, Campus da Betânia, Sobral, CE, Brasil.
authors: Lúcia Betânia da Silva
authors: LB
authors: Andrade
mesh_terms: D000818:Animals; D006189:Haemonchus / Q000187:drug effects*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology*; D010063:Ovum / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D012639:Seeds
chemical_list: D006460:Hemolysin Proteins; D037102:Lectins; D010936:Plant Extracts; D011480:Protease Inhibitors