title: Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease.
abstract: We report a sensitive and selective approach for the DNA methyltransferase (MTase) activity assay and MTase inhibitor screening by coupling the fluorescence quenching of graphene oxide with site-specific cleavage of a restriction endonuclease.
authors: Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China. zhangh@njnu.edu.cn cxcai@njnu.edu.cn.
authors: Lijuan
authors: L
authors: Ji
authors: Zhewei
authors: Z
authors: Cai
authors: Yingdan
authors: Y
authors: Qian
authors: Ping
authors: P
authors: Wu
authors: Hui
authors: H
authors: Zhang
authors: Chenxin
authors: C
authors: Cai
mesh_terms: D015257:DNA-Cytosine Methylases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010087:Oxides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D010087:Oxides; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D006108:Graphite; D015257:DNA-Cytosine Methylases