title: [Changes of platelet mitochondria in rats with tourniquet-induced limb ischemia- reperfusion injury].
abstract: OBJECTIVE
  To investigate the changes of platelet mitochondria in rats with tourniquet-induced limb ischemia-reperfusion (IR) injury.
  
  
  METHODS
  Thirty SD rats were randomized equally into 5 groups including a control group and 4 limb IR injury groups for blood sampling at 2, 6, 12, or 24 h following IR injury induced by tourniquet on the thighs for 4 h. Platelet was separated from the blood samples for measurement of ATP content, mitochondrial membrane potentials, plasma cytochrome C level, and hydroperoxides.
  
  
  RESULTS
  Compared with the control group, the rats with tourniquet-induced limb IR injury showed significantly decreased ATP content, lowered mitochondrial membrane potential, and increased plasma cytochrome C and hydroperoxide levels in the platelets at 2 and 6 h following the injury (P<0.05 or 0.01). These alterations recovered partially but remained significantly different from the control levels at 12 h (P<0.05 or 0.01) until full recovery at 24 h. Limb IR injury did not cause significant variations of the platelet counts.
  
  
  CONCLUSIONS
  Tourniquet-induced limb IR injury can cause mitochondrial damage in the platelets, which occurs mainly in the early stage (6 h) and recovers gradually afterwards without significant impact on platelet counts.
authors: Department of Anesthesiology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China. E-mail: jianxingzhang_edu@163.com.
authors: Jianxing
authors: J
authors: Zhang
authors: Hongying
authors: H
authors: Li
authors: Guobin
authors: G
authors: Zhou
authors: Qing
authors: Q
authors: Wang
authors: Zhen
authors: Z
authors: Li
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000473:pathology*; D045304:Cytochromes c / Q000097:blood; D006861:Hydrogen Peroxide / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000473:pathology*; D010976:Platelet Count; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury; D014111:Tourniquets
chemical_list: D000255:Adenosine Triphosphate; D045304:Cytochromes c; D006861:Hydrogen Peroxide