title: Identification of diverse innate lymphoid cells in human decidua.
abstract: Innate lymphoid cells (ILCs) are developmentally related cells that play an important role in innate defenses and tissue remodeling. So far, only natural killer (NK) cells have been identified and functionally characterized in human decidua where they contribute to induction of immune suppression, neo-angiogenesis, and tissue building/remodeling. The presence of other ILC subsets in human decidua has not been yet characterized. Here we identify in human decidua, during early pregnancy, two subsets of decidual group 3 ILC (ILC3), including lymphoid tissue inducer (LTi)-like cells and natural cytotoxicity receptors (NCRs)(+)ILC3 and interferon-(IFN)γ-producing ILC1, different from NK cells. Decidual LTi-like cells produced interleukin -17 (IL-17) and tumor necrosis factor (TNF), while NCR(+)ILC3 released IL-22 and IL-8. Importantly, NCR(+)ILC3 and LTi-like cells established functional interactions with stromal cells. Decidual LTi-like cells differentiated into NCR(+)ILC3, whereas they marginally contributed to NK cell generation. Our data suggest that decidual ILC3 may play a role in innate defenses and in vessel and tissue building, thus contributing to maintenance of pregnancy.
authors: Dipartimento di Medicina Sperimentale, Università di Genova, Genova, Italy.
authors: P
authors: P
authors: Vacca
authors: 1] Dipartimento di Medicina Sperimentale, Università di Genova, Genova, Italy [2] IRCCS AOU San Martino-IST, Genova, Italy.
authors: E
authors: E
authors: Montaldo
authors: 1] Dipartimento di Medicina Sperimentale, Università di Genova, Genova, Italy [2] IRCCS AOU San Martino-IST, Genova, Italy.
authors: D
authors: D
authors: Croxatto
authors: 1] IRCCS AOU San Martino-IST, Genova, Italy [2] Istituto Giannina Gaslini, Genova, Italy.
authors: F
authors: F
authors: Loiacono
authors: Istituto Giannina Gaslini, Genova, Italy.
authors: F
authors: F
authors: Canegallo
authors: 1] IRCCS AOU San Martino-IST, Genova, Italy [2] Dipartimento di Neuroscienze, riabilitazione, oftalmologia, genetica e scienze materno-infantili, Università di Genova, Genova, Italy.
authors: P L
authors: PL
authors: Venturini
authors: Istituto Giannina Gaslini, Genova, Italy.
authors: L
authors: L
authors: Moretta
authors: 1] Dipartimento di Medicina Sperimentale, Università di Genova, Genova, Italy [2] IRCCS AOU San Martino-IST, Genova, Italy.
authors: M C
authors: MC
authors: Mingari
mesh_terms: D000954:Antigens, Surface / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology; D003656:Decidua / Q000166:cytology* / Q000276:immunology*; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000166:cytology; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D010641:Phenotype; D011247:Pregnancy
chemical_list: D000954:Antigens, Surface; D016207:Cytokines