title: Glioma tissue obtained by modern ultrasonic aspiration with a simple sterile suction trap for primary cell culture and pathological evaluation.
abstract: OBJECTIVE
  Ultrasonic aspiration is widely used in the resection of brain tumors. Nevertheless, tumor tissue fragments obtained by ultrasonic aspiration are usually discarded. In this study, we demonstrate that these fragments are possible sources of material for histopathological study and tissue culture and compare their microscopic features and viability in tissue culture of cavitron ultrasonic surgical aspirator tissue fragments.
  
  
  METHODS
  Brain tumor tissue collected by ultrasonic aspiration (CUSA EXcel®; Integra Radionics Inc.) in a simple sterile suction trap during resection was processed for primary cell culture. Cell viability and immunohistological markers were measured by the WST-1 test, microscopy and immunofluorescent evaluation.
  
  
  RESULTS
  Six gliomas are presented to demonstrate that these tissue fragments show good preservation of histological detail and tissue viability in culture.
  
  
  CONCLUSIONS
  Utilization of this material may facilitate pathological interpretation by providing a more representative sample of tumor histology as well as an adequate and sterile biosource of material for tissue culture studies.
authors: Department of Neurosurgery, University Hospital Münster, Münster, Germany.
authors: Juliane
authors: J
authors: Schroeteler
authors: Ralf
authors: R
authors: Reeker
authors: Eric
authors: E
authors: Suero Molina
authors: Benjamin
authors: B
authors: Brokinkel
authors: Markus
authors: M
authors: Holling
authors: Oliver M
authors: OM
authors: Grauer
authors: Volker
authors: V
authors: Senner
authors: Walter
authors: W
authors: Stummer
authors: Christian
authors: C
authors: Ewelt
mesh_terms: D001921:Brain / Q000473:pathology*; D001932:Brain Neoplasms / Q000473:pathology*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D061765:Endoscopic Ultrasound-Guided Fine Needle Aspiration; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans