title: Isolation, identification and arsenic-resistance of Acidithiobacillus ferrooxidans HX3 producing schwertmannite.
abstract: Schwertmannite, a ubiquitous mineral present in iron oxyhydroxides formed in iron- and sulfate-rich acid media, favors incorporation of some toxic anions in its structure. We reported an iron-oxidizing bacterial strain HX3 from a municipal sludge that facilitates the formation of pure schwertmannite in cultures. Ferrous iron oxidation by the isolated strain HX3 was optimum at an initial pH of 2.0-3.3 and temperature of 28-35°C. Pure schwertmannite was found through bacterial oxidation of ferrous iron at an initial pH2.8 and temperature 28°C. Following 16S rDNA gene sequence analysis the bacterial strain HX3 was identified as Acidithiobacillus ferrooxidans. The arsenic-resistance A. ferrooxidans HX3 showed the potential of environmental application in arsenic removal from the As(III)- and iron-rich acid sulfate waters directly by As(III) adsorption or the formation of schwertmannite in the environment.
authors: School of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China.. Electronic address: yqxu@yzu.edu.cn.
authors: Yiqun
authors: Y
authors: Xu
authors: School of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China.
authors: Ming
authors: M
authors: Yang
authors: School of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China.
authors: Ting
authors: T
authors: Yao
authors: School of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China.. Electronic address: hxxiong@yzu.edu.cn.
authors: Huixin
authors: H
authors: Xiong
mesh_terms: D001151:Arsenic / Q000633:toxicity*; D004352:Drug Resistance, Microbial; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D058085:Iron Compounds / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D013855:Thiobacillus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D058085:Iron Compounds; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C511969:schwertmannite; D001151:Arsenic
keywords: Acidithiobacillus ferrooxidans; Arsenic-resistance; Identification; Isolation; Schwertmannite