title: Mouse models of acute and chronic colitis.
abstract: Crohn's disease (CD) and ulcerative colitis are two main clinically defined forms of chronic inflammatory bowel disease (IBD). Our understanding of IBD depends largely on rodent models. DSS-induced intestinal inflammation in mice and T cell transfer colitis in SCID mice are most widely used and accepted models that can recapitulate the human diseases. Here, we provide detailed protocols of these two mouse models of experimentally induced intestinal inflammation. We also discuss the protocols for the isolation and analysis of inflammatory T cell from the colon.
authors: Department of Medicine and Cancer Center, CN-4153, Georgia Regents University, 1410 Laney-Walker Blvd, Augusta, GA, 30912, USA, smanicassamy@gru.edu.
authors: Santhakumar
authors: S
authors: Manicassamy
authors: Indumathi
authors: I
authors: Manoharan
mesh_terms: D000208:Acute Disease; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D002908:Chronic Disease; D003093:Colitis, Ulcerative / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D003106:Colon / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D057075:Enzyme Assays; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D009195:Peroxidase / Q000378:metabolism; D013194:Staining and Labeling; D046509:Tissue Culture Techniques; D020858:Tissue and Organ Harvesting
chemical_list: D018398:Homeodomain Proteins; C064658:RAG-1 protein; D009195:Peroxidase; D017493:Leukocyte Common Antigens