title: Analytical protocols based on LC-MS, GC-MS and CE-MS for nontargeted metabolomics of biological tissues.
abstract: Invasive, site-specific metabolite information could be better obtained from tissues. Hence, highly sensitive mass spectrometry-based metabolomics coupled with separation techniques are increasingly in demand in clinical research for tissue metabolomics application. Applying these techniques to nontargeted tissue metabolomics provides identification of distinct metabolites. These findings could help us to understand alterations at the molecular level, which can also be applied in clinical practice as screening markers for early disease diagnosis. However, tissues as solid and heterogeneous samples pose an additional analytical challenge that should be considered in obtaining broad, reproducible and representative analytical profiles. This manuscript summarizes the state of the art in tissue (human and animal) treatment (quenching, homogenization and extraction) for nontargeted metabolomics with mass spectrometry.
authors: CEMBIO (Center for Metabolomics & Bioanalysis), Facultad de Farmacia, Universidad San Pablo CEU, Campus Monteprincipe, Boadilla del Monte, 28668 Madrid, Spain.
authors: Shama
authors: S
authors: Naz
authors: Délia Chaves
authors: DC
authors: Moreira dos Santos
authors: Antonia
authors: A
authors: García
authors: Coral
authors: C
authors: Barbas
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D016591:Histocytological Preparation Techniques; D006801:Humans; D055432:Metabolomics / Q000379:methods*