title: [Ketamine induces apoptosis of human uroepithelial SV-HUC-1 cells].
abstract: OBJECTIVE
  To determine the effect of ketamine on the apoptosis of human uroepithelial cells (SVHUC-1) and the pathogenesis of ketamine-associated cystitis.
  
  
  METHODS
  SV-HUC-1 cells were cultured under various concentrations of ketamine and differenttime. Flow cytometry was used to analyze the rate of cell apoptosis. The protein levels of Bax, Bcl-2, pro-caspase-3, and cleaved caspase-3 were detected by Western blot.
  
  
  RESULTS
  Compared with the control group, the apoptotic rate of ketamine cultured SV-HUC-1 cells increased. The expression of Bax increased, Bcl-2 expression decreased, and Bax/Bcl-2 in the ketamine cultured SV-HUC-1 cells was significantly higher. The protein level of pro-caspase-3 was significantly lower, and that of cleaved caspase-3 was significantly higher than that in the control group (P<0.05), positively correlated with the dose of ketamine and time of culture (P<0.05).
  
  
  CONCLUSIONS
  Ketamine can induce the apoptosis of SV-HUC-1 cells in a dose and time dependent manner.
authors: Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors: Liang
authors: L
authors: Huang
authors: Zhengyan
authors: Z
authors: Tang
authors: Dongjie
authors: D
authors: Li
authors: Guilin
authors: G
authors: Wang
authors: Ke
authors: K
authors: Huang
authors: Jie
authors: J
authors: Zhou
authors: Yonglin
authors: Y
authors: Li
authors: Benmin
authors: B
authors: Gao
authors: Junjie
authors: J
authors: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line / Q000187:drug effects; D004847:Epithelial Cells / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D007649:Ketamine / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D019459:Urothelium / Q000166:cytology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C000595707:BCL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D007649:Ketamine; C505407:CASP3 protein, human; D053148:Caspase 3