title: In vitro screening antibacterial activity of Bidens pilosa Linné and Annona crassiflora Mart. against oxacillin resistant Staphylococcus aureus (ORSA) from the aerial environment at the dental clinic.
abstract: Currently multiresistant Staphylococcus aureus is one common cause of infections with high rates of morbidity and mortality worldwide, which directs scientific endeavors in search for novel antimicrobials. In this study, nine extracts from Bidens pilosa (root, stem, flower and leaves) and Annona crassiflora (rind fruit, stem, leaves, seed and pulp) were obtained with ethanol: water (7:3, v/v) and their in vitro antibacterial activity evaluated through both the agar diffusion and broth microdilution methods against 60 Oxacillin Resistant S. aureus (ORSA) strains and against S. aureus ATCC6538. The extracts from B. pilosa and A. crassiflora inhibited the growth of the ORSA isolates in both methods. Leaves of B. pilosa presented mean of the inhibition zone diameters significantly higher than chlorexidine 0.12% against ORSA, and the extracts were more active against S. aureus ATCC (p < 0.05). Parallel, toxicity testing by using MTT method and phytochemical screening were assessed, and three extracts (B. pilosa, root and leaf, and A. crassiflora, seed) did not evidence toxicity. On the other hand, the cytotoxic concentrations (CC50 and CC90) for other extracts ranged from 2.06 to 10.77 mg/mL. The presence of variable alkaloids, flavonoids, tannins and saponins was observed, even though there was a total absence of anthraquinones. Thus, the extracts from the leaves of B. pilosa revealed good anti-ORSA activity and did not exhibit toxicity.
authors: Microbiology and Immunology Department, Biomedical Science Institute, Federal University of Alfenas, Alfenas, MG, Brazil.
authors: Jeferson Junior da
authors: JJ
authors: Silva
authors: Microbiology and Immunology Department, Biomedical Science Institute, Federal University of Alfenas, Alfenas, MG, Brazil.
authors: Cláudio Daniel
authors: CD
authors: Cerdeira
authors: Microbiology and Immunology Department, Biomedical Science Institute, Federal University of Alfenas, Alfenas, MG, Brazil.
authors: Juliana Moscardini
authors: JM
authors: Chavasco
authors: Microbiology and Immunology Department, Biomedical Science Institute, Federal University of Alfenas, Alfenas, MG, Brazil.
authors: Ana Beatriz Pugina
authors: AB
authors: Cintra
authors: Microbiology and Immunology Department, Biomedical Science Institute, Federal University of Alfenas, Alfenas, MG, Brazil.
authors: Carla Brigagão Pacheco da
authors: CB
authors: Silva
authors: Microbiology and Immunology Department, Biomedical Science Institute, Federal University of Alfenas, Alfenas, MG, Brazil.
authors: Andreia Natan de
authors: AN
authors: Mendonça
authors: Microbiology and Immunology Department, Biomedical Science Institute, Federal University of Alfenas, Alfenas, MG, Brazil.
authors: Tati
authors: T
authors: Ishikawa
authors: Research Laboratory of Genetics and Molecular Biology, University José do Rosário Vellano, Alfenas, MG, Brazil.
authors: Marcelo Fabiano Gomes
authors: MF
authors: Boriollo
authors: Microbiology and Immunology Department, Biomedical Science Institute, Federal University of Alfenas, Alfenas, MG, Brazil.
authors: Jorge Kleber
authors: JK
authors: Chavasco
mesh_terms: D031041:Annona / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D031176:Bidens / Q000737:chemistry*; D003740:Dental Clinics; D004783:Environmental Microbiology; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D018440:beta-Lactam Resistance / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts