title: Cryptosporidiosis in broiler chickens in Zhejiang Province, China: molecular characterization of oocysts detected in fecal samples.
abstract: Cryptosporidium is one of the most important parasites in poultry, and this pathogen can infect more than 30 avian species. The present study investigated the infection rate of Cryptosporidium among broiler chicken flocks. A total of 385 fecal samples from broiler chickens in 7 regions of Zhejiang Province collected from November 2010 to January 2012 were examined by microscopy. Thirty-eight (10%) samples were positive for Cryptosporidium infection, and 3 genotypes (Cryptosporidium baileyi, Cryptosporidium meleagridis, and avian genotype II) were identified by PCR and sequencing. A phylogenetic tree of the isolates was analyzed. These results suggest that cryptosporidiosis is widespread in poultry in Zhejiang Province, and is a potential threat to public health as well as the economy. This is the first report about the infection rate and molecular characterization of Cryptosporidium in broiler chickens in Zhejiang.
authors: Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
authors: Lengmei
authors: L
authors: Wang
authors: Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
authors: Xue
authors: X
authors: Xue
authors: Hangzhou College of Commerce, Zhejiang Gongshang University, Hangzhou, Zhejiang 310012, PR China.
authors: Jianqiu
authors: J
authors: Li
authors: Faculty of Life Science and Technology, Ningbo University, Ningbo, Zhejiang 315211, PR China.
authors: Qianjin
authors: Q
authors: Zhou
authors: Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
authors: Yingchao
authors: Y
authors: Yu
authors: Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
authors: Aifang
authors: A
authors: Du
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000469:parasitology*; D002681:China / Q000453:epidemiology; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000469:parasitology; D003458:Cryptosporidium / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics; D005243:Feces / Q000469:parasitology; D016266:Food Parasitology; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D033661:Oocysts / Q000737:chemistry*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000453:epidemiology / Q000469:parasitology*; D015995:Prevalence; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D021521:Ribotyping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S