title: Placental miR-1301 is dysregulated in early-onset preeclampsia and inversely correlated with maternal circulating leptin.
abstract: BACKGROUND
  miRNAs are small non-coding RNAs important for the regulation of mRNA in many organs including placenta. Adipokines and specifically leptin are known to be dysregulated in preeclampsia, but little is known regarding their regulation by miRNAs during pregnancy.
  
  
  METHODS
  We performed high-throughput sequencing of small RNAs in placenta from 72 well-defined patients: 23 early-onset preeclampsia (PE), 26 late-onset PE and 23 controls. The regulation of some miRNAs was confirmed on qRT-PCR. Maternal circulating levels and placental mRNA of leptin, resistin and adiponectin were measured using Bio-Plex and qRT-PCR.
  
  
  RESULTS
  We found that miR-1301, miR-223 and miR-224 expression was downregulated in early-onset PE, but not in late-onset PE, compared to controls. In silico analysis predicted the leptin gene (LEP) to be a target for all three miRNAs. Indeed, we found significant correlation between maternal circulating levels of leptin and placental LEP expression. In addition, we found a significant inverse correlation between maternal circulating leptin/placental LEP expression and placental miR-1301 expression levels. Interestingly, placental expression of miR-1301 was also correlated with newborn weight percentile and inversely correlated with both maternal systolic and diastolic blood pressure prior to delivery.
  
  
  CONCLUSIONS
  Our results confirm that placenta is a major site of LEP expression during pregnancy. It further suggests that miR-1301 could be involved in the regulation of leptin during pregnancy and may play a role in early-onset PE.
  
  
  CONCLUSIONS
  miR-1301 is dysregulated in early-onset preeclampsia and could possibly play a role in the regulation of leptin during pregnancy.
authors: The Biotechnology Centre of Oslo, University of Oslo, P.O. Box 1125, Blindern, N-0317 Oslo, Norway; Faculty of Medicine, University of Oslo, P.O. Box 1125, Blindern, N-0317 Oslo, Norway; Department of Gynaecology and Obstetrics, Oslo University Hospital, P.O. Box 4956, Nydalen, N-0424, Norway.
authors: M S
authors: MS
authors: Weedon-Fekjær
authors: Dept. of Medical Genetics, Oslo University Hospital, P.O. Box 4956, Nydalen, 0424 Oslo, Norway.
authors: Y
authors: Y
authors: Sheng
authors: Department of Gynaecology and Obstetrics, Oslo University Hospital, P.O. Box 4956, Nydalen, N-0424, Norway.
authors: M
authors: M
authors: Sugulle
authors: The Biotechnology Centre of Oslo, University of Oslo, P.O. Box 1125, Blindern, N-0317 Oslo, Norway; Department of Gynaecology and Obstetrics, Oslo University Hospital, P.O. Box 4956, Nydalen, N-0424, Norway.
authors: G M
authors: GM
authors: Johnsen
authors: Experimental and Clinical Research Center, a Joint Cooperation Between the Charité Medical Faculty and the Max-Delbrueck Center for Molecular Medicine, Berlin, Lindenbergerweg 80, 13125 Berlin, Germany; Franz-Volhard Klinik, HELIOS-Klinik, Berlin, Germany.
authors: F
authors: F
authors: Herse
authors: Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK.
authors: C W
authors: CW
authors: Redman
authors: Dept. of Medical Genetics, Oslo University Hospital, P.O. Box 4956, Nydalen, 0424 Oslo, Norway.
authors: R
authors: R
authors: Lyle
authors: Experimental and Clinical Research Center, a Joint Cooperation Between the Charité Medical Faculty and the Max-Delbrueck Center for Molecular Medicine, Berlin, Lindenbergerweg 80, 13125 Berlin, Germany; Franz-Volhard Klinik, HELIOS-Klinik, Berlin, Germany.
authors: R
authors: R
authors: Dechend
authors: Faculty of Medicine, University of Oslo, P.O. Box 1125, Blindern, N-0317 Oslo, Norway; Department of Gynaecology and Obstetrics, Oslo University Hospital, P.O. Box 4956, Nydalen, N-0424, Norway. Electronic address: UXNNAF@ous-hf.no.
authors: A C
authors: AC
authors: Staff
mesh_terms: D000293:Adolescent; D000328:Adult; D001794:Blood Pressure; D015992:Body Mass Index; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020738:Leptin / Q000097:blood*; D035683:MicroRNAs / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D055815:Young Adult
chemical_list: D020738:Leptin; D035683:MicroRNAs; C572423:MIRN1301 microRNA, human; C525067:MIR223, human; C546140:MIRN224 microRNA, human
keywords: Early-onset preeclampsia; High throughput sequencing; Late-onset preeclampsia; Leptin; MicroRNA; miR-1301