title: Renin gene polymorphisms in bangladeshi hypertensive population.
abstract: OBJECTIVE
  Linkages of renin gene polymorphisms with hypertension have been implicated in several populations with contrasting results. Present study aims to assess the pattern of renin gene polymorphisms in Bangladeshi hypertensive individuals.
  
  
  METHODS
  Introns 1, 9 of renin gene and 4063 bases upstream of promoter sequence of renin gene were amplified from the genomic DNA of the total 124 (hypertensive and normotensive) subjects using respective primers. Polymerase chain reaction-based restriction fragment length polymorphisms were performed using BglI, MboI and TaqI restriction enzymes.
  
  
  RESULTS
  Homozygosity was common in renin gene regarding BglI (bb=48.4%, Bb=37.9%, BB=13.7%, χ (2) =1.91, P>0.05), TaqI (TT=81.5%, Tt=14.5%, tt=4.0%, χ (2) =7.50, P<0.01) and MboI (mm=63.7%, Mm=32.3%, MM=4.0%, χ (2) =0.00, P>0.05) polymorphisms among total study population. For BglI and TaqI genotype distribution, hypertensive subjects (BglI: χ (2) =6.66, P<0.05; TaqI: χ (2) = 10.28, P<0.005) significantly deviate from Hardy-Weinberg Equilibrium law compared to normotensive subjects (BglI: χ (2) =0.51, P>0.05; TaqI: χ (2) =0.20, P>0.05). On the other hand, with respect to MboI polymorphisms of renin gene, only normotensive subjects deviate from the law (patients: χ (2) =1.28, P>0.05; vs controls: χ (2) =6.81, P<0.01). In the context of allelic frequency, common T allele was clearly prevalent (T frequency=0.86, t frequency = 0.14) for TaqI, but rare alleles b and m were more frequent for both BglI (b frequency=0.69, B frequency=0.31) and MboI (m frequency=0.80 M frequency=0.20) polymorphisms, respectively.
  
  
  CONCLUSIONS
  Thus, we report that Bangladeshi hypertensive subjects did not show any distinct pattern of renin gene polymorphisms compared to their healthy control subjects with regard to their genotypic and allelic frequencies.
authors: 1. Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000;
authors: Rownock
authors: R
authors: Afruza
authors: 1. Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000;
authors: Laila N
authors: LN
authors: Islam
authors: 2. Department of Cardiology, Bangabandhu Sheikh Mujib Medical University;
authors: Sajal
authors: S
authors: Banerjee
authors: 3. Laboratory Medicine Division, Apollo Hospitals, Dhaka, Bangladesh;
authors: Md Mahbub
authors: MM
authors: Hassan
authors: 4. Laboratory of Animal Biochemistry, Faculty of Applied Biological Sciences, Gifu University, Japan.
authors: Fumiaki
authors: F
authors: Suzuki
authors: 1. Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000;
authors: Ahm Nurun
authors: AN
authors: Nabi
keywords: BglI polymorphism; Hardy-Weinberg equilibrium.; MboI polymorphism; Renin gene polymorphism; TaqI polymorphism; association analysis; genetic variation; hypertension; renin