title: miR-101 promotes breast cancer cell apoptosis by targeting Janus kinase 2.
abstract: OBJECTIVE
  microRNA-101 (miR-101) is down-regulated in several cancers. In this study, we explored the effects of dysregulated miR-101 on breast cancer cells and the underlying mechanisms.
  
  
  METHODS
  miR-101 level was quantified by real-time RT-PCR. Cell viability was analyzed by MTT assay. Apoptosis was detected by flow cytometry and TUNEL assay. Moreover, the level of protein expression was determined by Western blot.
  
  
  RESULTS
  miR-101 level was markedly reduced in both the human breast cancer samples and cultured breast cancer cell lines (MCF-7, MDA-MB-231). Overexpression of miR-101 inhibited the proliferation and promoted the apoptosis in cultured MCF-7 and MDA-MB-231 cells, which were reversed by co-transfection of AMO-101, the inhibitor of miR-101. We validated Janus kinase 2 (Jak2) as a direct target of miR-101. Knockdown of Jak2 induced apoptosis in cultured breast cancer cells. Moreover, the level of miR-101 is negatively correlated with Jak2 in breast cancer tissues and cell lines.
  
  
  CONCLUSIONS
  miR-101 suppressed proliferation and promoted apoptosis in breast cancer cells by targeting Jak2. These findings indicate that manipulation of miR-101 expression may represent a novel therapeutic strategy in the treatment of breast cancer.
authors: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), Harbin Medical University, Harbin, P. R.China.
authors: Lu
authors: L
authors: Wang
authors: Linqiang
authors: L
authors: Li
authors: Rui
authors: R
authors: Guo
authors: Xuelian
authors: X
authors: Li
authors: Ying
authors: Y
authors: Lu
authors: Xiaoxiang
authors: X
authors: Guan
authors: Samuel Chege
authors: SC
authors: Gitau
authors: Leimin
authors: L
authors: Wang
authors: Chaoqian
authors: C
authors: Xu
authors: Baofeng
authors: B
authors: Yang
authors: Hongli
authors: H
authors: Shan
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000201:enzymology / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D053614:Janus Kinase 2 / Q000235:genetics*; D035683:MicroRNAs / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D053614:Janus Kinase 2