title: [Enhanced sensitivity of leukemia cell line KG-1a to activated immune cell-mediated cytolysis after treated with resveratrol].
abstract: OBJECTIVE
  To explore the enhanced sensitivity of leukemia cell line KG-1a to activated immune cell-mediated cytolysis after treated with resveratrol.
  
  
  METHODS
  The value of 50% inhibition concentration (IC₅₀) for KG-1a by resveratrol was analyzed using trypan blue staining. Peripheral blood mononuclear cells were separated, and then activated by interleukin (IL)-2 and IL-15. The sensitivity of KG-1a treated with and without resveratrol to activated immune cell-mediated cytolysis was assayed by lactate dehydrogenase (LDH) -releasing assay. The expression of tumor necrosis factor related apoptosis inducing ligand (TRAIL) on the surface of activated immune cells and its receptors (DR4/5 and DcR1/2) on the surface of KG-1a were detected by flow cytometry.
  
  
  RESULTS
  Resveratrol could inhibit the proliferation of KG-1a and IC50 at 24 h was 25 mmol/L. At a ratio of 10:1 or 20:1 between effect and target, the cytolytic rates of treated KG-1a by activated immune cells were (55.80 ± 10.88)% and (72.31 ± 13.06)%, significantly higher than (24.96 ± 9.25)% and (37.93 ± 5.21)% of untreated KG-1a (P<0.05). The expression of DR5 on the surface of KG-1a treated with resveratrol was (9.05 ± 3.57)%, significantly higher than (3.11 ± 0.54)% of untreated KG-1a (P<0.05). Conversely, the expression of DcR1 on the surface of treated KG-1a was (13.23 ± 3.56)%, lower than (53.75 ± 10.51)% of KG-1a (P<0.05). When TRAIL pathway on the surface of activated immune cells was blocked, the cytolytic rates of treated KG-1a were (35.97 ± 6.36)% and (49.80 ± 10.68)%, significantly lower than (52.92 ± 6.98)% and (70.73 ± 9.79)% of untreated KG-1a (P<0.05) at the same ratio of effector and target.
  
  
  CONCLUSIONS
  Resveratrol could enhance cytolytic sensitivity of KG-1a by activated immune cells through TRAIL pathway.
authors: Department of Laboratory Medicine, Guangdong Provincial No.2 People's Hospital, Guangzhou 510317, China.
authors: Liangshan
authors: L
authors: Hu
authors: Department of Laboratory Medicine, Guangdong Provincial No.2 People's Hospital, Guangzhou 510317, China.
authors: Huawen
authors: H
authors: Yang
authors: Department of Laboratory Medicine, Guangdong Provincial No.2 People's Hospital, Guangzhou 510317, China.
authors: Lihua
authors: L
authors: Li
authors: Department of Laboratory Medicine, Guangdong Provincial No.2 People's Hospital, Guangzhou 510317, China.
authors: Zhihong
authors: Z
authors: Zhang
authors: Department of Laboratory Medicine, Guangdong Provincial No.2 People's Hospital, Guangzhou 510317, China.
authors: Xiaolin
authors: X
authors: Fang
authors: Department of Laboratory Medicine, Guangdong Provincial No.2 People's Hospital, Guangzhou 510317, China.
authors: Donglin
authors: D
authors: Cao
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008297:Male; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism; D053320:Receptors, Tumor Necrosis Factor, Member 10c / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism
chemical_list: D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D013267:Stilbenes; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D000077185:Resveratrol