title: Effect of local application of an antibody against brain-derived neurotrophic factor on neuroma formation after transection of the inferior alveolar nerve in the rat.
abstract: This study aimed to examine the contributions of brain-derived neurotrophic factor (BDNF) at the injury site toward neuroma formation and nerve regeneration after inferior alveolar nerve transection. Histological analysis confirmed neuroma formation at 2 weeks after complete transection of the inferior alveolar nerve. A local administration of an antibody to BDNF inhibited connective tissue proliferation at the injury site and promoted nerve fiber integrity. Fluorogold labeling showed a significantly higher number of labeled cells in the trigeminal ganglion in the anti-BDNF-treated group compared with the vehicle control group. In-situ hybridization histochemistry showed intense signals for tropomyosin receptor kinase B mRNA in the area of the injury site containing fibrous or granular tissue in the anti-BDNF-treated group. In contrast, these signals were close to the detection limit in the area of the perineurium in intact nerve trunks, indicating that the signals were expressed by fibroblasts within the connective tissue. These findings suggest that antagonization of endogenous BDNF induced by nerve injury reduces neuroma formation, without inhibiting damaged axon regeneration.
authors: aDivision of Oral Anatomy bDivision of Orthodontics cDivision of Dental Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Yessenia M
authors: YM
authors: Valverde Guevara
authors: Hiroyuki
authors: H
authors: Yoshikawa
authors: Isao
authors: I
authors: Saito
authors: Takeyasu
authors: T
authors: Maeda
authors: Kenji
authors: K
authors: Seo
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D019208:Brain-Derived Neurotrophic Factor / Q000276:immunology / Q000378:metabolism*; D003390:Cranial Nerve Neoplasms / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D005347:Fibroblasts / Q000473:pathology / Q000502:physiology; D017403:In Situ Hybridization; D008297:Male; D008340:Mandibular Nerve / Q000473:pathology / Q000503:physiopathology*; D009416:Nerve Regeneration / Q000502:physiology*; D009463:Neuroma / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000378:metabolism; D013266:Stilbamidines; D061221:Trigeminal Nerve Injuries / Q000150:complications / Q000473:pathology / Q000503:physiopathology*
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D000906:Antibodies; D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D013266:Stilbamidines; D020813:Receptor, trkB