title: Neuregulin-1 increases connexin-40 and connexin-45 expression in embryonic stem cell-derived cardiomyocytes.
abstract: Neuregulin-1 (NRG-1) is a vital factor involved in heart development. NRG-1 up-regulated connexin-40 (Cx40) in mice fetal cardiomyocytes has been reported, while the effect of NRG-1 on expression of connexins in embryonic stem cells-derived cardiomyocytes (ESCMs) is limited studied. The process of cardiomyocytes differentiated from embryonic stem cells with or without NRG-1 treatment was observed continuously. Exposure to NRG-1 increased the expression of Cx40 and connexin-45 (Cx45) in ESCMs, while the expression of connexin-43 was unchanged regardless of NRG-1 treatment Western blot analysis also confirmed that the expression of Cx40 and Cx45 in the beating foci was increased in the presence of NRG-1. These results indicate that connexins are differentially regulated by exogenous NRG-1 during cardiomyocytic differentiation of embryonic stem cells.
authors: Department of Cardiology, Nanjing Chest Hospital, 215 Guangzhou Road, Nanjing, 210029, China, wangzhi1977nj@hotmail.com.
authors: Zhi
authors: Z
authors: Wang
authors: Jin
authors: J
authors: Huang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D017630:Connexins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D032383:Myocytes, Cardiac / Q000378:metabolism*; D020890:Neuregulin-1 / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D000097003:Gap Junction alpha-5 Protein
chemical_list: D017630:Connexins; D017931:DNA Primers; D020890:Neuregulin-1; D012333:RNA, Messenger; D000097003:Gap Junction alpha-5 Protein; C490581:Nrg1 protein, mouse; C082920:connexin 45