title: Oral chronic graft-versus-host disease: analysis of dendritic cells subpopulations.
abstract: The graft-versus-host disease is the major cause of morbidity and mortality in patients who have undergone hematopoietic stem cell transplantation. Aiming at contributing to the understanding of the role of myeloid and plasmacytoid dendritic cells, and natural killer cells in chronic graft-versus-host disease, we examined biopsies of jugal mucosa of 26 patients with acute myeloid leukemia who had undergone allogenic hematopoietic stem cell transplantation. Half of these patients developed oral chronic graft-versus-host disease. Microscopic sections were immunohistochemically stained for anti-CD1a, anti-CD123 and anti-CD56. We calculated the number of immunostained cells in the corium per square millimeter and applied the Mann-Whitney test. Results showed a statistically significant increase of myeloid dendritic cells (CD1a+; p=0,02) and natural killer cells (CD56; p=0,04) in patients with oral chronic graft-versus-host disease. CD123 immunostaining showed no statistical difference between groups. It was concluded that myeloid dendritic cells and natural killer cells participate in the development of oral chronic graft-versus-host disease.
authors: Hospital Fundação Amaral Carvalho, Jaú, SP, Brazil.
authors: Clara Marino Espricigo
authors: CM
authors: Botari
authors: Hospital Fundação Amaral Carvalho, Jaú, SP, Brazil.
authors: Adauto José Ferreira
authors: AJ
authors: Nunes
authors: Hospital Fundação Amaral Carvalho, Jaú, SP, Brazil.
authors: Mair Pedro de
authors: MP
authors: Souza
authors: Hospital Fundação Amaral Carvalho, Jaú, SP, Brazil.
authors: Erica Sinara Lenharo
authors: ES
authors: Orti-Raduan
authors: Hospital Fundação Amaral Carvalho, Jaú, SP, Brazil.
authors: Ana Gabriela
authors: AG
authors: Salvio
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D017209:Apoptosis; D001706:Biopsy; D002452:Cell Count; D002908:Chronic Disease; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology / Q000473:pathology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000276:immunology / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000276:immunology / Q000473:pathology; D008297:Male; D009061:Mouth Mucosa / Q000276:immunology / Q000473:pathology*; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D015703:Antigens, CD