title: Increased serum concentrations of pentosidine are related to presence and severity of coronary artery disease.
abstract: BACKGROUND
  There are limited data regarding the contribution of advanced glycation end products (AGEs) in the presence of coronary artery disease (CAD). We investigated whether serum pentosidine and Nε-carboxymethyllysine (CML) were related to the presence and the severity of CAD.
  
  
  METHODS
  69 Tunisian patients with CAD (≥ 50% obstruction in ≥ 1 coronary artery), 32 Tunisian patients without CAD but with potential cardiovascular risk factors and 60 Tunisian control subjects were included in a cross-sectional study. Patients were classified as CAD and non CAD patients according to angiographic results. The severity of CAD was assessed using the Gensini score. Serum pentosidine and CML were measured by LC-MS/MS.
  
  
  RESULTS
  Serum pentosidine and CML concentrations were significantly higher in non-CAD patients vs control subjects (P<0.001). Serum pentosidine concentrations were significantly higher in CAD patients vs non-CAD patients (P<0.001). A multiple logistic regression analysis demonstrated that pentosidine was independently associated with the presence of CAD (OR=1.52, 95% CI: 1.12-2.07, P=0.007). The area under curve (AUC) determined by ROC analysis was 0.74 (95% CI: 0.64-0.84, P<0.001) and the optimal cut-off value of pentosidine to predict the presence of CAD was 3.2 μmol/mol Lys, with 64% sensitivity and 78% specificity. Furthermore, in a multivariate stepwise regression analysis, pentosidine was independently correlated with Gensini score (standardized β= 0.46, 95% CI: 0.70-1.99, P<0.001).
  
  
  CONCLUSIONS
  High concentrations of pentosidine show the presence and the severity of CAD with high sensitivity.
authors: Laboratory of Biochemistry, LR12ES05, Faculty of Medicine, University of Monastir, Tunisia. Electronic address: mohsen.kerkeni@yahoo.fr.
authors: Mohsen
authors: M
authors: Kerkeni
authors: Laboratory of Paediatric Biology and Research, American Memorial Hospital, University Hospital of Reims, Faculty of Medicine, Reims, France; Laboratory of Biochemistry and Molecular Biology, UMR CNRS/URCA n°7369, Faculty of Medicine, Reims, France.
authors: Izabella Santos
authors: IS
authors: Weiss
authors: Laboratory of Paediatric Biology and Research, American Memorial Hospital, University Hospital of Reims, Faculty of Medicine, Reims, France; Laboratory of Biochemistry and Molecular Biology, UMR CNRS/URCA n°7369, Faculty of Medicine, Reims, France.
authors: Stephane
authors: S
authors: Jaisson
authors: Laboratory of Biochemistry, CHU-Farhat Hached, Sousse, Tunisia.
authors: Azza
authors: A
authors: Dandana
authors: Department of Cardiology-University Hopital A. Mami, Ariana, Tunisia.
authors: Faouzi
authors: F
authors: Addad
authors: Laboratory of Paediatric Biology and Research, American Memorial Hospital, University Hospital of Reims, Faculty of Medicine, Reims, France; Laboratory of Biochemistry and Molecular Biology, UMR CNRS/URCA n°7369, Faculty of Medicine, Reims, France.
authors: Philippe
authors: P
authors: Gillery
authors: Laboratory of Biochemistry, LR12ES05, Faculty of Medicine, University of Monastir, Tunisia.
authors: Mohamed
authors: M
authors: Hammami
mesh_terms: D000368:Aged; D019540:Area Under Curve; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000097:blood* / Q000000981:diagnostic imaging; D023921:Coronary Stenosis / Q000097:blood* / Q000000981:diagnostic imaging; D003430:Cross-Sectional Studies; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood*; D006801:Humans; D016015:Logistic Models; D008239:Lysine / Q000031:analogs & derivatives* / Q000097:blood; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016017:Odds Ratio; D011237:Predictive Value of Tests; D012372:ROC Curve; D012720:Severity of Illness Index; D014416:Tunisia; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D017127:Glycation End Products, Advanced; C048496:N(6)-carboxymethyllysine; D001120:Arginine; C062187:pentosidine; D008239:Lysine
keywords: Advanced glycation end products; Coronary artery disease; Gensini score; Pentosidine