title: Description of Mariniphaga anaerophila gen. nov., sp. nov., a facultatively aerobic marine bacterium isolated from tidal flat sediment, reclassification of the Draconibacteriaceae as a later heterotypic synonym of the Prolixibacteraceae and description of the family Marinifilaceae fam. nov.
abstract: A mesophilic, chemoheterotrophic bacterium, strain Fu11-5(T), was isolated from tidal-flat sediment from Tokyo Bay, Chiba, Japan. Cells of strain Fu11-5(T) were facultatively aerobic, Gram-negative, non-sporulating, non-motile and rod-shaped (1.9-6.9 µm long). Strain Fu11-5(T) grew optimally at 35-37 °C and pH 6.5-7.0 and with 1-2% (w/v) NaCl. Oxygen and l-cysteine were used as an alternative electron acceptor and donor, respectively. Strain Fu11-5(T) also grew fermentatively on some pentoses, hexoses and disaccharides and soluble starch. Succinic acid was the major end product from d-glucose. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain Fu11-5(T) was affiliated with the order Bacteroidales, and its nearest neighbours were members of the genera Meniscus, Prolixibacter, Sunxiuqinia, Mangrovibacterium and Draconibacterium, with 87-91% sequence similarity. Cell morphology, optimum growth temperature and utilization of sugars of strain Fu11-5(T) distinguished the strain from phylogenetically related bacteria. On the basis of its phenotypic features and phylogenetic position, a novel genus and species are proposed to accommodate strain Fu11-5(T), with the name Mariniphaga anaerophila gen. nov., sp. nov. The type strain of Mariniphaga anaerophila is strain Fu11-5(T) ( =JCM 18693(T) =NBRC 109408(T) =DSM 26910(T)). We also propose to combine the family Draconibacteriaceae into the family Prolixibacteraceae as a later heterotypic synonym and to place the distinct sublineage of the genus Marinifilum in the family Marinifilaceae fam. nov.
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Iino
authors.affiliation: NITE Biological Resource Center (NBRC), Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Mori
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Kudo
authors.affiliation: NITE Biological Resource Center (NBRC), Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Ken-Ichiro
authors.initials: KI
authors.lastname: Suzuki
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Moriya
authors.initials: M
authors.lastname: Ohkuma
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7