title: [Protective effects of total epimedium flavonoids against QA-induced toxicity in SH-SY5Y cells].
abstract: OBJECTIVE
  To investigate the protective effects and its mechanisms of total epimedium flavonoids (TEF) against Quindinince acid (QA)-induced toxicity in SH-SY5Y cells.
  
  
  METHODS
  The MT assay was used to detect the toxicity of QA and the cell viability of pretreatment of TEF and co-incubation with QA in SH-SY5Y cells; Cell apoptosis was observed by Hochest33258 staining; SOD and GSH-Px activities as well as MDA content were measured by colorimetric method; The mitochondrial membrane potential (delta psi m) and intracellular free calcium concentration ([Ca2+]i) were monitored by FCM.
  
  
  RESULTS
  TEF increased the cell viability of SH-SY5Y cells, reduced apoptosis cells significantly, improved SOD and GSH-Px activities, decreased MDA content, increased the mitochondrial membrane potential and reduced intracellular free calcium concentration( [Ca2+]i ).
  
  
  CONCLUSIONS
  TEF has significant protective effects against QA-induced toxicity in SH-SY5Y cells,the action mechanism may be associated with resisting oxidative stress, decreasing the [Ca2]i and up-regulating delta psi m.
authors.forename: Sheng-mei
authors.initials: SM
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yan-xi
authors.initials: YX
authors.lastname: Shi
authors.forename: Chuan-xia
authors.initials: CX
authors.lastname: Ju
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.forename: Feng-xiao
authors.initials: FX
authors.lastname: Li
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology; D017378:Quinolinic Acid / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D018696:Neuroprotective Agents; D017378:Quinolinic Acid; D002118:Calcium