title: Sinomonas notoginsengisoli sp. nov., isolated from the rhizosphere of Panax notoginseng.
abstract: A Gram-positive, aerobic, non-motile and pale yellow colour actinobacterial strain, designated SYP-B575(T), was isolated from rhizosphere soil of Panax notoginseng. The optimal growth of the strain was found to occur at 28 °C, pH 7.0 and without NaCl. Phylogenetic analysis indicated that strain SYP-B575(T) clearly belongs to the genus Sinomonas and should be considered as a candidate of novel species within this genus. The 16S rRNA gene sequence similarities between strain SYP-B575(T) and the other Sinomonas type strains ranged from 97.3 to 96.0%. The predominant isoprenoid quinone was identified as MK-9(H2) and the major fatty acids were identified as anteiso-C(15:0) and anteiso-C(17:0). The polar lipids were found to consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and glycolipids. The major cell-wall amino acids were identified as Lys, Ala, Glu, Gly and Ser. The whole-cell sugars were identified as mannose, ribose, rhamnose, glucose and galactose. The G+C content of the genomic DNA was determined to be 66.6 mol%. The DNA-DNA relatedness values between SYP-B575(T) and its closest phylogenetic neighbours were lower than 35.5%. On the basis of this polyphasic taxonomic study, strain SYP-B575(T) represents a novel species of the genus Sinomonas, for which the name Sinomonas notoginsengisoli sp. nov. is proposed. The type strain is SYP-B575(T) (=DSM 27685(T) = KCTC 29237(T)).
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China.
authors.forename: Meng-Yue
authors.initials: MY
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.forename: Tian-Yuan
authors.initials: TY
authors.lastname: Zhang
authors.forename: Huan
authors.initials: H
authors.lastname: Xu
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.forename: Shan-Hui
authors.initials: SH
authors.lastname: Li
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Yi-Xuan
authors.initials: YX
authors.lastname: Zhang
mesh_terms: D000332:Aerobiosis; D000596:Amino Acids / Q000032:analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D053723:Panax notoginseng / Q000254:growth & development; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride