title: Prevalence of HPV 16 and HPV 18 lineages in Galicia, Spain.
abstract: Genetic variants of human papillomavirus types 16 and 18 (HPV16/18) could differ in their cancer risk. We studied the prevalence and association with high-grade cervical lesions of different HPV16/18 variant lineages in a case-control study including 217 cases (cervical intraepithelial neoplasia grade 2 or grade 3 or worse: CIN2 or CIN3+) and 116 controls (no CIN2 or CIN3+ in two-year follow-up). HPV lineages were determined by sequencing the long control region (LCR) and the E6 gene. Phylogenetic analysis of HPV16 confirmed that isolates clustered into previously described lineages: A (260, 87.5%), B (4, 1.3%), C (8, 2.7%), and D (25, 8.4%). Lineage D/lineage A strains were, respectively, detected in 4/82 control patients, 19/126 CIN3+ cases (OR = 3.1, 95%CI: 1.0-12.9, p = 0.04), 6/1 glandular high-grade lesions (OR = 123, 95%CI: 9.7-5713.6, p<0.0001), and 4/5 invasive lesions (OR = 16.4, 95%CI: 2.2-113.7, p = 0.002). HPV18 clustered in lineages A (32, 88.9%) and B (4, 11.1%). Lineage B/lineage A strains were respectively detected in 1/23 control patients and 2/5 CIN3+ cases (OR = 9.2, 95%CI: 0.4-565.4, p = 0.12). In conclusion, lineages A of HPV16/18 were predominant in Spain. Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A. Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant. Large well-designed studies are needed before the application of HPV lineage detection in clinical settings.
authors.affiliation: Department of Microbiology, University Hospital of Vigo, Vigo, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Pérez
authors.affiliation: Department of Microbiology, University Hospital of Ourense, Ourense, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Cid
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital of Vigo, Vigo, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Iñarrea
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital of Ourense, Ourense, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Pato
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital of Vigo, Vigo, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Lamas
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital of Ourense, Ourense, Spain.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Couso
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital of Vigo, Vigo, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Gil
authors.affiliation: Department of Pathology, University Hospital of Vigo, Vigo, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Alvarez
authors.affiliation: Department of Microbiology, University Hospital of Vigo, Vigo, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Rey
authors.affiliation: Department of Microbiology, University Hospital of Vigo, Vigo, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: López-Miragaya
authors.affiliation: Department of Virology, University Hospital of Asturias, Oviedo, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Melón
authors.affiliation: Department of Virology, University Hospital of Asturias, Oviedo, Spain.
authors.forename: María de
authors.initials: Md
authors.lastname: Oña
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D014644:Genetic Variation; D060005:Genotyping Techniques; D052162:Human papillomavirus 16 / Q000235:genetics*; D052161:Human papillomavirus 18 / Q000235:genetics*; D006801:Humans; D013030:Spain; D002583:Uterine Cervical Neoplasms / Q000821:virology
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