title: Platelet-rich plasma derived from bone marrow aspirate promotes new cementum formation.
abstract: BACKGROUND
  This study evaluates the influence of platelet-rich plasma derived from bone marrow aspirate (PRP-BMA) on the healing of periodontal fenestration defects in rats.
  
  
  METHODS
  Periodontal fenestration defects were surgically created in the mandibles of 40 rats. The animals were randomly divided into two groups, control and PRP-BMA, in which defects were filled with blood clot or PRP-bma, respectively. Animals were euthanized at either 10 or 30 days post-surgery. Histologic, histometric, and immunohistochemical analyses were performed. Percentage of new bone area (NBA), area of bone trabeculae (ABT), new cementum (NC), and extension of remaining defect were histometrically evaluated. Proliferating cell nuclear antigen (PCNA), bone sialoprotein (BSP), osteocalcin (OCN), and tartrate-resistant acid phosphatase (TRAP) immunohistochemical staining were performed. Immunolabeled cells were quantified. Data were statistically analyzed (analysis of variance; Tukey, P <0.05).
  
  
  RESULTS
  At 10 days, control and PRP-BMA groups presented similar amounts of NBA and ABT; NC formation was not observed. At 30 days, control and PRP-BMA groups presented similar amounts of NBA and ABT; the PRP-BMA group showed NC formation with collagen fibers inserted obliquely or perpendicularly to the root surface. NC formation was not observed in any control group specimen. PRP- BMA presented higher numbers of PCNA-positive and BSP-positive cells than control at 10 and 30 days post-surgery. No significant differences in the number of either OCN-positive or TRAP-positive cells were observed between groups at 10 or 30 days.
  
  
  CONCLUSIONS
  PRP-BMA promoted NC formation with a functional periodontal ligament when applied at experimental periodontal fenestration defects.
authors.affiliation: Department of Surgery and Integrated Clinic, Dental School of Araçatuba, São Paulo State University, Araçatuba, São Paulo, Brazil.
authors.forename: Maria J H
authors.initials: MJ
authors.lastname: Nagata
authors.forename: Natália
authors.initials: N
authors.lastname: de Campos
authors.forename: Michel R
authors.initials: MR
authors.lastname: Messora
authors.forename: Carolina S
authors.initials: CS
authors.lastname: Santinoni
authors.forename: Suely R M
authors.initials: SR
authors.lastname: Bomfim
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Fucini
authors.forename: Natália M
authors.initials: NM
authors.lastname: Pola
authors.forename: Adrieli P
authors.initials: AP
authors.lastname: Neves
authors.forename: Juliano M
authors.initials: JM
authors.lastname: de Almeida
authors.forename: Letícia H
authors.initials: LH
authors.lastname: Theodoro
authors.forename: Edilson
authors.initials: E
authors.lastname: Ervolino
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D016301:Alveolar Bone Loss / Q000473:pathology / Q000628:therapy*; D000818:Animals; D001777:Blood Coagulation / Q000502:physiology; D001854:Bone Marrow Cells / Q000502:physiology*; D001861:Bone Regeneration / Q000502:physiology; D025021:Cementogenesis / Q000502:physiology*; D003094:Collagen / Q000648:ultrastructure; D003238:Connective Tissue / Q000473:pathology; D003739:Dental Cementum / Q000473:pathology; D007249:Inflammation; D058574:Integrin-Binding Sialoprotein / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D008336:Mandibular Diseases / Q000473:pathology / Q000628:therapy*; D009336:Necrosis; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000502:physiology; D010513:Periodontal Ligament / Q000473:pathology; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors
chemical_list: C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D007527:Isoenzymes; D018809:Proliferating Cell Nuclear Antigen; D015675:Osteocalcin; D003094:Collagen; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: Bone marrow; dental cementum; periodontics; platelet-rich plasma; rats; wound healing