title: In situ assessment of effects of the bromide- and fluoride-incorporating adhesive systems on biofilm and secondary caries.
abstract: OBJECTIVE
  This in situ study assessed the effects of adhesive systems containing or not fluoride and/or the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the microbiological composition of dental biofilm and enamel demineralization.
  
  
  METHODS
  During two phases of 14 days, ten volunteers wore intraoral palatal appliances containing two slabs of human enamel according to a double-blind, crossover design. The slabs were randomly restored using a composite resin and one of the following adhesive systems: All-Bond SE(TM) (self-etch, fluoride/MDPB free adhesive, AB) and Clearfl Protect Bond (self-etch containing fluoride and MDPB adhesive, CB). The biofilm formed on the slabs was analyzed with regard to total and mutans streptococci and lactobacilli counts. Demineralization represented by integrated area of hardness × lesion depth Delta S ( ΔS) was determined on enamel by analysis of cross-sectional microhardness, at 20 and 70 μm from the restoration margin. Data were analyzed by ANOVA.
  
  
  RESULTS
  No statistically significant difference was found either in enamel demineralization or in the microbiological composition of dental biofilm.
  
  
  CONCLUSIONS
  All adhesive systems containing or not fluoride and/or MDPB tested were unable to inhibit secondary caries in the in situ model used in the present research.
authors.affiliation: Student, Post-graduation Program in Dentistry, Faculty of Pharmacy Dentistry and Nursing, Federal University of Ceara, Ceara Brazil.
authors.forename: Suyane Maria Luna Cruz de
authors.initials: SM
authors.lastname: Vasconcelos
authors.affiliation: Assistant Professor, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore MD, USA.
authors.forename: Mary Anne Sampaio de
authors.initials: MA
authors.lastname: Melo
authors.affiliation: Undergraduate Student , Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Ceara, Brazil.
authors.forename: Joao Paulo Marques Saraiva
authors.initials: JP
authors.lastname: Wenceslau
authors.affiliation: Associate Professor, The School of Dentistry of Sobral; Professor of the Post-graduation Program in Dentistry, Federal University of Ceara Ceara, Brazil.
authors.forename: Iriana Carla Junqueira
authors.initials: IC
authors.lastname: Zanin
authors.affiliation: Associate Professor, Department of Operative Dentistry, Faculty of Pharmacy Dentistry and Nursing, Federal University of Ceara, Ceara Brazil.
authors.forename: Haroldo Cesar Pinheiro
authors.initials: HC
authors.lastname: Beltrao
authors.affiliation: Associate Professor, Department of Operative Dentistry, Faculty of Pharmacy Dentistry and Nursing, Federal University of Ceara, Ceara Brazil.
authors.forename: Carlos Augusto Oliveira
authors.initials: CA
authors.lastname: Fernandes
authors.affiliation: Associate Professor, Department of Public Health, Ceara State University, Ceara Brazil.
authors.forename: Paulo Cesar de
authors.initials: PC
authors.lastname: Almeida
authors.affiliation: Associate Professor, Department of Operative Dentistry, Faculty of Pharmacy Dentistry and Nursing; Professor of the Post-graduation Program in Dentistry, Federal University of Ceara, Ceara, Brazil.
authors.forename: Lidiany Karla Azevedo
authors.initials: LK
authors.lastname: Rodrigues
mesh_terms: D058491:Bacterial Load / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D002327:Cariostatic Agents / Q000008:administration & dosage* / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D018592:Cross-Over Studies; D003731:Dental Caries / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D003743:Dental Enamel / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D003764:Dental Materials / Q000737:chemistry; D017220:Dentin-Bonding Agents / Q000737:chemistry; D004311:Double-Blind Method; D005459:Fluorides / Q000008:administration & dosage* / Q000737:chemistry; D006244:Hardness; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects; D008689:Methacrylates / Q000737:chemistry; D011726:Pyridinium Compounds / Q000008:administration & dosage* / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry*; D013295:Streptococcus mutans / Q000187:drug effects; D017001:Tooth Demineralization / Q000209:etiology / Q000382:microbiology / Q000473:pathology; D055815:Young Adult
chemical_list: C074864:All-Bond system; D002327:Cariostatic Agents; C503075:Clearfil Protect Bond; D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; C444701:Filtek Z250; D008689:Methacrylates; D011726:Pyridinium Compounds; D019279:Resin Cements; C089392:methacryloyloxydodecylpyridinium bromide; D005459:Fluorides