title: Polymerase chain reaction-based assays for the diagnosis of human brucellosis.
abstract: Polymerase chain reaction (PCR) is an in vitro technique for the nucleic acid amplification, which is commonly used to diagnose infectious diseases. The use of PCR for pathogens detection, genotyping and quantification has some advantages, such as high sensitivity, high specificity, reproducibility and technical ease. Brucellosis is a common zoonosis caused by Brucella spp., which still remains as a major health problem in many developing countries around the world. The direct culture and immunohistochemistry can be used for detecting infection with Brucella spp. However, PCR has the potential to address limitations of these methods. PCR are now one of the most useful assays for the diagnosis in human brucellosis. The aim of this review was to summarize the main PCR techniques and their applications for diagnosis and follow-up of patients with brucellosis. Moreover, advantages or limitation of the different PCR methods as well as the evaluation of PCR results for treatment and follow-up of human brucellosis were also discussed.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhanli
authors.initials: Z
authors.lastname: Wang
authors.forename: Yaxian
authors.initials: Y
authors.lastname: Zhang
authors.forename: Liyun
authors.initials: L
authors.lastname: Bai
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Chunfang
authors.initials: C
authors.lastname: Liu
authors.forename: An
authors.initials: A
authors.lastname: Ma
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
mesh_terms: D002002:Brucella / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis*; D016903:Drug Monitoring / Q000379:methods; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*
references.citation: Harefuah. 2006 Nov;145(11):836-40, 860
references.pmid: 17183958
references.citation: J Clin Microbiol. 2008 Jan;46(1):385-7
references.pmid: 17977995
references.citation: J Med Virol. 1990 Feb;30(2):85-91
references.pmid: 2156009
references.citation: Clin Microbiol Rev. 2000 Oct;13(4):559-70
references.pmid: 11023957
references.citation: J Clin Microbiol. 2010 Mar;48(3):697-702
references.pmid: 20053863
references.citation: East Mediterr Health J. 2009 Sep-Oct;15(5):1068-74
references.pmid: 20214119
references.citation: Clin Microbiol Infect. 2008 Dec;14(12):1128-34
references.pmid: 19046166
references.citation: Eur J Clin Microbiol Infect Dis. 2005 Dec;24(12):842-5
references.pmid: 16341519
references.citation: J Microbiol Methods. 2008 Oct;75(2):375-8
references.pmid: 18675856
references.citation: J Clin Microbiol. 1999 Dec;37(12):4163-6
references.pmid: 10565954
references.citation: Ann Agric Environ Med. 2013;20(2):233-8
references.pmid: 23772567
references.citation: FEMS Microbiol Lett. 2006 Apr;257(1):7-16
references.pmid: 16553826
references.citation: Jpn J Infect Dis. 2011;64(4):272-6
references.pmid: 21788700
references.citation: Vet Microbiol. 2011 Dec 29;154(1-2):152-5
references.pmid: 21782356
references.citation: BMC Infect Dis. 2013 Mar 21;13:145
references.pmid: 23517532
references.citation: Mol Med. 1997 Nov;3(11):734-9
references.pmid: 9407549
references.citation: J Clin Microbiol. 2007 Apr;45(4):1211-8
references.pmid: 17267626
references.citation: J Clin Microbiol. 1995 Jun;33(6):1640-2
references.pmid: 7650203
references.citation: PLoS One. 2009;4(2):e4526
references.pmid: 19225565
references.citation: J Clin Microbiol. 1994 Nov;32(11):2660-6
references.pmid: 7852552
references.citation: Can J Microbiol. 2008 May;54(5):352-7
references.pmid: 18449219
references.citation: J Med Microbiol. 2005 Aug;54(Pt 8):727-730
references.pmid: 16014425
references.citation: J Clin Microbiol. 1995 Mar;33(3):615-7
references.pmid: 7538508
references.citation: Ann Saudi Med. 2000 May-July;20(3-4):224-8
references.pmid: 17322662
references.citation: Vet Microbiol. 2002 Dec 20;90(1-4):435-46
references.pmid: 12414163
references.citation: Appl Environ Microbiol. 2010 Sep;76(17):5837-45
references.pmid: 20639360
references.citation: Crit Rev Microbiol. 2005;31(4):191-6
references.pmid: 16417200
references.citation: Pol J Microbiol. 2011;60(1):27-33
references.pmid: 21630571
references.citation: J Clin Microbiol. 1999 May;37(5):1654-5
references.pmid: 10328689
references.citation: Rev Med Inst Mex Seguro Soc. 2007 Nov-Dec;45(6):615-22
references.pmid: 18593545
references.citation: J Clin Microbiol. 1995 Dec;33(12):3087-90
references.pmid: 8586678
references.citation: J Microbiol Methods. 2013 Feb 15;92(2):103-7
references.pmid: 23174277
references.citation: J Clin Microbiol. 1998 Nov;36(11):3217-22
references.pmid: 9774568
references.citation: Mikrobiyol Bul. 2011 Jul;45(3):392-400
references.pmid: 21935772
references.citation: Vet Microbiol. 2009 May 28;137(1-2):156-64
references.pmid: 19200666
references.citation: Jpn J Infect Dis. 2007 Nov;60(6):362-6
references.pmid: 18032835
references.citation: J Health Popul Nutr. 2013 Jun;31(2):218-22
references.pmid: 23930340
references.citation: Prilozi. 2010;31(1):65-89
references.pmid: 20703184
references.citation: Int J Med Microbiol. 2009 Dec;299(8):563-73
references.pmid: 19560966
references.citation: Nucleic Acids Res. 1997 Aug 15;25(16):3235-41
references.pmid: 9241236
references.citation: J Microbiol Methods. 2010 Jan;80(1):112-4
references.pmid: 19887090
references.citation: Microbes Infect. 2006 Mar;8(3):860-5
references.pmid: 16483820
references.citation: Spine (Phila Pa 1976). 2010 Nov 15;35(24):E1392-6
references.pmid: 21030888
references.citation: J Microbiol Biotechnol. 2011 Jan;21(1):89-92
references.pmid: 21301197
references.citation: Asian Pac J Trop Med. 2013 Jun;6(6):453-6
references.pmid: 23711705
references.citation: J Clin Microbiol. 2002 Apr;40(4):1475-80
references.pmid: 11923376
references.citation: Clin Infect Dis. 2005 Jan 15;40(2):260-4
references.pmid: 15655745
references.citation: BMC Infect Dis. 2003 Apr 28;3:5
references.pmid: 12718759
references.citation: FEMS Immunol Med Microbiol. 2002 Oct 11;34(2):147-51
references.pmid: 12381466
references.citation: PLoS One. 2013;8(3):e58353
references.pmid: 23520501
references.citation: BMC Microbiol. 2011 Mar 24;11:60
references.pmid: 21435217
references.citation: J Infect Dev Ctries. 2012 Jun 15;6(6):495-500
references.pmid: 22706192
references.citation: J Clin Microbiol. 2001 Apr;39(4):1661-4
references.pmid: 11283112
references.citation: Adv Exp Med Biol. 2003;529:451-3
references.pmid: 12756807
references.citation: Vet Microbiol. 2002 Dec 20;90(1-4):209-27
references.pmid: 12414145
references.citation: J Neurol Neurosurg Psychiatry. 2005 Jul;76(7):1025-7
references.pmid: 15965220
references.citation: J Clin Microbiol. 2007 Sep;45(9):2923-8
references.pmid: 17634297
references.citation: Ann Trop Med Parasitol. 2002 Jun;96(4):397-403
references.pmid: 12171621
references.citation: Clin Microbiol Infect. 2005 Sep;11(9):713-8
references.pmid: 16104985
references.citation: J Microbiol Methods. 2009 Jul;78(1):66-70
references.pmid: 19410609
references.citation: Med Sci Monit. 2005 Nov;11(11):MT69-74
references.pmid: 16258407
references.citation: Expert Rev Mol Diagn. 2004 Jan;4(1):115-23
references.pmid: 14711354
references.citation: Mikrobiyol Bul. 2002 Jul-Oct;36(3-4):271-6
references.pmid: 12838660
references.citation: Clin Diagn Virol. 1995 Aug;4(2):113-20
references.pmid: 15566833
references.citation: J Clin Microbiol. 1998 Sep;36(9):2443-6
references.pmid: 9705371
references.citation: Jpn J Infect Dis. 2007 May;60(2-3):137-9
references.pmid: 17515651
references.citation: J Infect Public Health. 2012 Apr;5(2):189-94
references.pmid: 22541267
references.citation: Diagn Microbiol Infect Dis. 2011 Nov;71(3):294-6
references.pmid: 21855249
references.citation: Mol Cell Probes. 2011 Apr-Jun;25(2-3):126-9
references.pmid: 21232598
references.citation: Clin Vaccine Immunol. 2008 Feb;15(2):293-6
references.pmid: 18077622
references.citation: Int J Prev Med. 2011 Jul;2(3):170-7
references.pmid: 21811660
references.citation: Int J Antimicrob Agents. 2010 Nov;36 Suppl 1:S12-7
references.pmid: 20692128
references.citation: Expert Rev Anti Infect Ther. 2011 Jul;9(7):833-45
references.pmid: 21810055
references.citation: J Clin Microbiol. 1996 Feb;34(2):477-8
references.pmid: 8789045
references.citation: Croat Med J. 2010 Aug;51(4):306-13
references.pmid: 20718083
references.citation: Diagn Microbiol Infect Dis. 2005 Sep;53(1):1-7
references.pmid: 16054326
references.citation: J Clin Microbiol. 1997 Nov;35(11):2927-30
references.pmid: 9350761
references.citation: Diagn Microbiol Infect Dis. 2007 Sep;59(1):23-32
references.pmid: 17532591
references.citation: BMC Microbiol. 2008 Jun 02;8:86
references.pmid: 18518958
references.citation: Clin Lab. 2003;49(9-10):487-505
references.pmid: 14572205
references.citation: J Bacteriol. 1992 Dec;174(23):7778-83
references.pmid: 1360006
references.citation: Exp Hematol. 2002 Jun;30(6):503-12
references.pmid: 12063017
references.citation: J Clin Microbiol. 2008 Oct;46(10):3484-7
references.pmid: 18716225
references.citation: Int J Infect Dis. 2010 Jun;14(6):e469-78
references.pmid: 19910232
references.citation: Pol J Microbiol. 2009;58(1):15-9
references.pmid: 19469281
references.citation: BMC Microbiol. 2009 Apr 07;9:66
references.pmid: 19351390
references.citation: Vet Microbiol. 1996 Jul;51(1-2):169-78
references.pmid: 8828133
references.citation: Clin Diagn Lab Immunol. 2000 Sep;7(5):835-9
references.pmid: 10973465
references.citation: Clin Infect Dis. 2006 May 1;42(9):1266-73
references.pmid: 16586386
references.citation: Mil Med. 2013 Jan;178(1):88-94
references.pmid: 23356125
references.citation: J Appl Bacteriol. 1990 Aug;69(2):216-27
references.pmid: 2272943
references.citation: PCR Methods Appl. 1993 Dec;3(3):S18-29
references.pmid: 8118393