title: Molecular analysis of the cold tolerant Antarctic nematode, Panagrolaimus davidi.
abstract: Isolated and established in culture from the Antarctic in 1988, the nematode Panagrolaimus davidi has proven to be an ideal model for the study of adaptation to the cold. Not only is it the best-documented example of an organism surviving intracellular freezing but it is also able to undergo cryoprotective dehydration. As part of an ongoing effort to develop a molecular understanding of this remarkable organism, we have assembled both a transcriptome and a set of genomic scaffolds. We provide an overview of the transcriptome and a survey of genes involved in temperature stress. We also explore, in silico, the possibility that P. davidi will be susceptible to an environmental RNAi response, important for further functional studies.
authors.affiliation: British Antarctic Survey, Natural Environment Research Council, Cambridge, United Kingdom.
authors.forename: Michael A S
authors.initials: MA
authors.lastname: Thorne
authors.affiliation: Transdisciplinary Research Integration Center, Research Organization of Information and Systems, Tokyo, Japan; National Institute of Genetics, Mishima, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kagoshima
authors.affiliation: British Antarctic Survey, Natural Environment Research Council, Cambridge, United Kingdom.
authors.forename: Melody S
authors.initials: MS
authors.lastname: Clark
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors.forename: Craig J
authors.initials: CJ
authors.lastname: Marshall
authors.affiliation: Department of Zoology, University of Otago, Dunedin, New Zealand.
authors.forename: David A
authors.initials: DA
authors.lastname: Wharton
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D005615:Freezing; D059647:Gene-Environment Interaction; D049751:Genome, Helminth / Q000502:physiology*; D009348:Nematoda / Q000502:physiology*; D059467:Transcriptome / Q000502:physiology*
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