title: Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice.
abstract: Transferring gut microbiota from one individual to another may enable researchers to "humanize" the gut of animal models and transfer phenotypes between species. To date, most studies of gut microbiota transfer are performed in germ-free mice. In the studies presented, it was tested whether an antibiotic treatment approach could be used instead. C57BL/6 mice were treated with ampicillin prior to inoculation at weaning or eight weeks of age with gut microbiota from lean or obese donors. The gut microbiota and clinical parameters of the recipients was characterized one and six weeks after inoculation. The results demonstrate, that the donor gut microbiota was introduced, established, and changed the gut microbiota of the recipients. Six weeks after inoculation, the differences persisted, however alteration of the gut microbiota occurred with time within the groups. The clinical parameters of the donor phenotype were partly transmissible from obese to lean mice, in particularly β cell hyperactivity in the obese recipients. Thus, a successful inoculation of gut microbiota was not age dependent in order for the microbes to colonize, and transferring different microbial compositions to conventional antibiotic-treated mice was possible at least for a time period during which the microbiota may permanently modulate important host functions.
authors.affiliation: 1] Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark [2].
authors.forename: Merete
authors.initials: M
authors.lastname: Ellekilde
authors.affiliation: 1] Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark [2].
authors.forename: Ellika
authors.initials: E
authors.lastname: Selfjord
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Christian S
authors.initials: CS
authors.lastname: Larsen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Maja
authors.initials: M
authors.lastname: Jakesevic
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Ida
authors.initials: I
authors.lastname: Rune
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Britt
authors.initials: B
authors.lastname: Tranberg
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, 1958 Frederiksberg C, Denmark.
authors.forename: Finn K
authors.initials: FK
authors.lastname: Vogensen
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, 1958 Frederiksberg C, Denmark.
authors.forename: Dennis S
authors.initials: DS
authors.lastname: Nielsen
authors.affiliation: National Food Institute, Technical University of Denmark, 2860 Søborg, Denmark.
authors.forename: Martin I
authors.initials: MI
authors.lastname: Bahl
authors.affiliation: National Food Institute, Technical University of Denmark, 2860 Søborg, Denmark.
authors.forename: Tine R
authors.initials: TR
authors.lastname: Licht
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Axel K
authors.initials: AK
authors.lastname: Hansen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.
authors.forename: Camilla H F
authors.initials: CH
authors.lastname: Hansen
mesh_terms: D000667:Ampicillin / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D064307:Microbiota
chemical_list: D000900:Anti-Bacterial Agents; D000667:Ampicillin
references.citation: Diabetologia. 2006 Sep;49(9):2105-8
references.pmid: 16816951
references.citation: Gastroenterology. 2012 Oct;143(4):913-6.e7
references.pmid: 22728514
references.citation: J Clin Invest. 2010 Dec;120(12):4332-41
references.pmid: 21099116
references.citation: Gut Microbes. 2013 May-Jun;4(3):241-5
references.pmid: 23549457
references.citation: Nature. 2006 Dec 21;444(7122):1022-3
references.pmid: 17183309
references.citation: Cell. 2014 Jan 16;156(1-2):123-33
references.pmid: 24439373
references.citation: Acta Diabetol. 2011 Dec;48(4):257-273
references.pmid: 21964884
references.citation: Lab Anim. 2010 Oct;44(4):283-9
references.pmid: 20713427
references.citation: Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):11070-5
references.pmid: 16033867
references.citation: FEMS Microbiol Ecol. 2003 May 1;44(1):131-6
references.pmid: 19719658
references.citation: Nature. 2013 Aug 29;500(7464):541-6
references.pmid: 23985870
references.citation: Appl Environ Microbiol. 1998 Oct;64(10):3854-9
references.pmid: 9758810
references.citation: Am J Clin Nutr. 1999 May;69(5):1035S-1045S
references.pmid: 10232646
references.citation: PLoS One. 2012;7(3):e34043
references.pmid: 22479515
references.citation: Science. 2013 Sep 6;341(6150):1241214
references.pmid: 24009397
references.citation: FEMS Microbiol Lett. 2012 Dec;337(1):38-47
references.pmid: 22967145
references.citation: Nature. 2006 Dec 21;444(7122):1027-31
references.pmid: 17183312
references.citation: Gut. 2010 Dec;59(12):1635-42
references.pmid: 20926643
references.citation: Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15718-23
references.pmid: 15505215
references.citation: PLoS One. 2010 Aug 16;5(8):e12191
references.pmid: 20808947
references.citation: QJM. 2009 Nov;102(11):781-4
references.pmid: 19726581
references.citation: Appl Environ Microbiol. 1976 Mar;31(3):359-75
references.pmid: 938032
references.citation: Res Vet Sci. 2014 Apr;96(2):241-50
references.pmid: 24556473
references.citation: Exp Anim. 2005 Oct;54(5):437-45
references.pmid: 16365521
references.citation: Endocrinology. 2013 Oct;154(10):3515-24
references.pmid: 23867214
references.citation: Diabetologia. 2012 Aug;55(8):2285-94
references.pmid: 22572803
references.citation: J Pediatr Gastroenterol Nutr. 2009 Apr;48 Suppl 2:S56-7
references.pmid: 19300127
references.citation: J Diabetes Res. 2013;2013:319321
references.pmid: 24369539
references.citation: Curr Opin Gastroenterol. 2010 Jan;26(1):5-11
references.pmid: 19901833
references.citation: PLoS One. 2010 Feb 05;5(2):e9085
references.pmid: 20140211