title: CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice.
abstract: Common methods for the generation of human embryonic-derived neural stem cells (hNSCs) result in cells with potentially compromised safety profiles due to maintenance of cells in conditions containing non-human proteins (e.g. in bovine serum or on mouse fibroblast feeders). Additionally, sufficient expansion of resulting hNSCs for scaling out or up in a clinically relevant time frame has proven to be difficult. Here, we report a strategy that produces hNSCs in completely "Xeno-Free" culture conditions. Furthermore, we have enriched the hNSCs for the cell surface marker CD133 via magnetic sorting, which has led to an increase in the expansion rate and neuronal fate specification of the hNSCs in vitro. Critically, we have also confirmed neural lineage specificity upon sorted hNSC transplantation into the immunodeficient NOD-scid mouse brain. The future use or adaptation of these protocols has the potential to better facilitate the advancement of pre-clinical strategies from the bench to the bedside.
authors.affiliation: Sue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USA; Anatomy & Neurobiology, University of California, Irvine, CA 92697-1750, USA.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Haus
authors.affiliation: Sue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USA; UCI Institute for Memory Impairments and Neurological Disorders (MIND), University of California, Irvine, CA 92697-1750, USA.
authors.forename: Hal X
authors.initials: HX
authors.lastname: Nguyen
authors.affiliation: Sue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USA; Anatomy & Neurobiology, University of California, Irvine, CA 92697-1750, USA.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Gold
authors.affiliation: Sue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USA; UCI Institute for Memory Impairments and Neurological Disorders (MIND), University of California, Irvine, CA 92697-1750, USA.
authors.forename: Noriko
authors.initials: N
authors.lastname: Kamei
authors.affiliation: Sue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USA; UCI Institute for Memory Impairments and Neurological Disorders (MIND), University of California, Irvine, CA 92697-1750, USA.
authors.forename: Harvey
authors.initials: H
authors.lastname: Perez
authors.affiliation: Centre for Stem Cell Biology, University of Sheffield, Sheffield S10 2TN, UK; Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK.
authors.forename: Harry D
authors.initials: HD
authors.lastname: Moore
authors.affiliation: Sue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USA; Physical and Medical Rehabilitation, University of California, Irvine, CA 92697-1750, USA; Anatomy & Neurobiology, University of California, Irvine, CA 92697-1750, USA; UCI Institute for Memory Impairments and Neurological Disorders (MIND), University of California, Irvine, CA 92697-1750, USA.
authors.forename: Aileen J
authors.initials: AJ
authors.lastname: Anderson
authors.affiliation: Sue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USA; Physical and Medical Rehabilitation, University of California, Irvine, CA 92697-1750, USA; Anatomy & Neurobiology, University of California, Irvine, CA 92697-1750, USA; UCI Institute for Memory Impairments and Neurological Disorders (MIND), University of California, Irvine, CA 92697-1750, USA. Electronic address: cummings@uci.edu.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Cummings
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D019070:Cell Lineage; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D053595:Embryonic Stem Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism*; D064593:Heterografts; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D058953:Neural Stem Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D055495:Neurogenesis; D010455:Peptides / Q000378:metabolism*; D010641:Phenotype; D013724:Teratoma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D015415:Biomarkers; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse
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