title: Characterisation of nine new polymorphic microsatellite loci in the reticulated glass frogHyalinobatrachium valerioi(Centrolenidae).
abstract: Here we document the development of thirteen novel microsatellite markers for the reticulated glass frogHyalinobatrachium valerioi(Centrolenidae). Nine of those markers were polymorphic and contained between 4 and 34 alleles per locus (mean = 20.3) in 138 individuals (91 males, 47 females) from the field site 'La Gamba', Costa Rica. Average observed heterozygosity was 0.76. Two loci (Hyval19 and Hyval21) significantly deviated from Hardy-Weinberg equilibrium. We did not find evidence for linkage disequilibrium among any of the loci. These markers will serve to identify the genetic mating system inH. valerioi, investigate gene flow between local populations, and reconstruct parent-offspring relationships for studies on individual mating and reproductive success. Therefore, these markers will serve to answer a wide range of scientific questions in conservation, behavioural ecology, and also evolutionary biology.
authors.affiliation: Department of Integrative Zoology, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria ; Department of Cognitive Biology, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Ringler
authors.affiliation: Department of Integrative Zoology, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Mangold
authors.affiliation: Department of Integrative Zoology, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Trenkwalder
authors.affiliation: Department of Integrative Zoology, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria ; Department of Tropical Ecology and Animal Biodiversity, University of Vienna, Rennweg 14, A-1030 Vienna, Austria.
authors.forename: Max
authors.initials: M
authors.lastname: Ringler
authors.affiliation: Department of Integrative Zoology, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria.
authors.forename: Walter
authors.initials: W
authors.lastname: Hödl
keywords: Costa Rica; Hyalinobatrachium valerioi; microsatellite markers; population genetics
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