title: Approach to reduction of blood atherogenicity.
abstract: We have earlier found that blood sera of patients with coronary heart disease (CHD) increase lipid levels in cells cultured from subendothelial intima of human aorta. We have also revealed that the ability of blood sera to raise intracellular cholesterol; that is, their atherogenicity is caused by at least modified low density lipoprotein (LDL) circulating in the blood of patients and autoantibodies to modified LDL. In the present work we have demonstrated significant impact of nonlipid factor(s) to blood atherogenicity. We have developed an approach to removal of nonlipid atherogenicity factor(s) from blood serum based on the use of immobilized LDL. This approach was used for extracorporeal perfusion of patient's blood through the column with immobilized LDL. Pilot clinical study confirmed the efficacy of this approach for prevention of coronary atherosclerosis progression.
authors.affiliation: Institute for Atherosclerosis Research, Skolkovo Innovation Center, P.O. Box No. 21, Moscow 121609, Russia ; Institute of General Pathology and Pathophysiology, Baltiyskaya Street 8, Moscow 125315, Russia.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Orekhov
authors.affiliation: Institute for Atherosclerosis Research, Skolkovo Innovation Center, P.O. Box No. 21, Moscow 121609, Russia ; Institute of General Pathology and Pathophysiology, Baltiyskaya Street 8, Moscow 125315, Russia.
authors.forename: Alexandra A
authors.initials: AA
authors.lastname: Melnichenko
authors.affiliation: Institute for Atherosclerosis Research, Skolkovo Innovation Center, P.O. Box No. 21, Moscow 121609, Russia ; Institute of General Pathology and Pathophysiology, Baltiyskaya Street 8, Moscow 125315, Russia ; Russian Cardiology Research and Production Center, 3rd Cherepkovskaya Street 15a, Moscow 121552, Russia.
authors.forename: Igor A
authors.initials: IA
authors.lastname: Sobenin
mesh_terms: D000328:Adult; D001011:Aorta / Q000166:cytology / Q000378:metabolism; D050197:Atherosclerosis / Q000097:blood* / Q000473:pathology; D001323:Autoantibodies / Q000276:immunology / Q000302:isolation & purification; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood; D002846:Chromatography, Affinity; D017023:Coronary Angiography; D003327:Coronary Disease / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D006435:Renal Dialysis; D012720:Severity of Illness Index
chemical_list: D001323:Autoantibodies; D008055:Lipids; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D002784:Cholesterol
references.citation: Arch Med Sci. 2013 Feb 21;9(1):151-8
references.pmid: 23515030
references.citation: Atherosclerosis. 1997 Apr;130(1-2):133-42
references.pmid: 9126657
references.citation: Atherosclerosis. 2014 Jan;232(1):204-10
references.pmid: 24401239
references.citation: Circ Res. 1990 Feb;66(2):311-20
references.pmid: 2297806
references.citation: J Altern Complement Med. 2013 Sep;19(9):759-66
references.pmid: 23484524
references.citation: Physiol Rev. 2013 Jul;93(3):1317-542
references.pmid: 23899566
references.citation: Curr Pharm Des. 2013;19(33):5909-28
references.pmid: 23438951
references.citation: J Clin Immunol. 2013 Feb;33(2):489-95
references.pmid: 23073618
references.citation: Atherosclerosis. 1998 May;138(1):183-95
references.pmid: 9678784
references.citation: PLoS One. 2013;8(2):e56782
references.pmid: 23460815
references.citation: Atherosclerosis. 1988 Aug;72(2-3):245-8
references.pmid: 3063269
references.citation: J Cardiovasc Transl Res. 2013 Apr;6(2):197-207
references.pmid: 23423864
references.citation: J Res Med Sci. 2013 Feb;18(2):89-93
references.pmid: 23914207
references.citation: Ther Apher. 1997 Aug;1(3):242-8
references.pmid: 10225746
references.citation: Nutr Metab Cardiovasc Dis. 2012 Jan;22(1):1-7
references.pmid: 22176921
references.citation: Arterioscler Thromb Vasc Biol. 2010 Dec;30(12):2311-6
references.pmid: 21084697
references.citation: Int Heart J. 2013;54(1):33-9
references.pmid: 23428922
references.citation: Curr Opin Lipidol. 2004 Jun;15(3):329-35
references.pmid: 15166790
references.citation: Lancet. 1986 Sep 13;2(8507):595-8
references.pmid: 2875319
references.citation: Recent Pat Cardiovasc Drug Discov. 2013 Apr;8(1):56-66
references.pmid: 23176379
references.citation: Redox Biol. 2013 Oct 08;1:483-91
references.pmid: 24251116
references.citation: Atheroscler Suppl. 2013 Jan;14(1):93-9
references.pmid: 23357149
references.citation: Ann Med. 1993 Dec;25(6):551-5
references.pmid: 8292305
references.citation: Acta Diabetol. 2008 Mar;45(1):1-6
references.pmid: 17823766
references.citation: Atherosclerosis. 2001 Nov;159(1):103-15
references.pmid: 11689212
references.citation: Circ Res. 1988 Mar;62(3):421-9
references.pmid: 3342473
references.citation: Biochem Biophys Res Commun. 1989 Aug 30;163(1):489-94
references.pmid: 2775281
references.citation: Exp Mol Pathol. 1997;64(3):127-45
references.pmid: 9439479
references.citation: Atherosclerosis. 1997 Nov;135(1):19-27
references.pmid: 9395269
references.citation: Exp Mol Pathol. 1995 Jun;62(3):166-72
references.pmid: 8612720
references.citation: Trends Cardiovasc Med. 2013 Aug;23(6):211-21
references.pmid: 23561795
references.citation: Curr Pharm Des. 2013;19(33):5954-62
references.pmid: 23438956
references.citation: Proc Natl Acad Sci U S A. 1989 Feb;86(4):1372-6
references.pmid: 2465552
references.citation: Nature. 2011 May 19;473(7347):317-25
references.pmid: 21593864
references.citation: Acta Clin Croat. 2010 Dec;49(4):429-39
references.pmid: 21830454
references.citation: Br J Nutr. 2012 Apr;107(7):1062-9
references.pmid: 22011476
references.citation: Am J Cardiol. 1987 Feb 15;59(5):495-6
references.pmid: 3812328
references.citation: Coron Artery Dis. 1993 Apr;4(4):379-85
references.pmid: 8261211
references.citation: Ther Apher. 2001 Aug;5(4):221-5
references.pmid: 11724504
references.citation: Arterioscler Thromb. 1991 Mar-Apr;11(2):316-26
references.pmid: 1998649
references.citation: Atherosclerosis. 2013 Aug;229(2):356-62
references.pmid: 23880187