title: Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield.
abstract: Mammary gland is made up of a branching network of ducts that end with alveoli which surrounds the lumen. These alveolar mammary epithelial cells (MEC) reflect the milk producing ability of farm animals. In this study, we have used 2D-DIGE and mass spectrometry to identify the protein changes in MEC during immediate early, peak and late stages of lactation and also compared differentially expressed proteins in MEC isolated from milk of high and low milk producing cows. We have identified 41 differentially expressed proteins during lactation stages and 22 proteins in high and low milk yielding cows. Bioinformatics analysis showed that a majority of the differentially expressed proteins are associated in metabolic process, catalytic and binding activity. The differentially expressed proteins were mapped to the available biological pathways and networks involved in lactation. The proteins up-regulated during late stage of lactation are associated with NF-κB stress induced signaling pathways and whereas Akt, PI3K and p38/MAPK signaling pathways are associated with high milk production mediated through insulin hormone signaling.
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal, India.
authors.forename: Jagadeesh
authors.initials: J
authors.lastname: Janjanam
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal, India.
authors.forename: Surender
authors.initials: S
authors.lastname: Singh
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal, India.
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Jena
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal, India.
authors.forename: Nishant
authors.initials: N
authors.lastname: Varshney
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Srujana
authors.initials: S
authors.lastname: Kola
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal, India.
authors.forename: Sudarshan
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal, India.
authors.forename: Jai K
authors.initials: JK
authors.lastname: Kaushik
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal, India.
authors.forename: Sunita
authors.initials: S
authors.lastname: Grover
authors.affiliation: Dairy Cattle Physiology Division, National Dairy Research Institute, Karnal, India.
authors.forename: Ajay K
authors.initials: AK
authors.lastname: Dang
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, India.
authors.forename: Manishi
authors.initials: M
authors.lastname: Mukesh
authors.affiliation: Dairy Cattle Physiology Division, National Dairy Research Institute, Karnal, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Prakash
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal, India.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Mohanty
mesh_terms: D000818:Animals; D002417:Cattle; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008892:Milk / Q000378:metabolism*; D008894:Milk Proteins / Q000235:genetics / Q000378:metabolism; D025341:Principal Component Analysis; D040901:Proteomics; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D008894:Milk Proteins
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