title: Impact of extraction parameters on the recovery of lipolytic activity from fermented babassu cake.
abstract: Enzyme extraction from solid matrix is as important step in solid-state fermentation to obtain soluble enzymes for further immobilization and application in biocatalysis. A method for the recovery of a pool of lipases from Penicillium simplicissimum produced by solid-state fermentation was developed. For lipase recovery different extraction solution was used and phosphate buffer containing Tween 80 and NaCl showed the best results, yielding lipase activity of 85.7 U/g and 65.7 U/g, respectively. The parameters with great impacts on enzyme extraction detected by the Plackett-Burman analysis were studied by Central Composite Rotatable experimental designs where a quadratic model was built showing maximum predicted lipase activity (160 U/g) at 25°C, Tween 80 0.5% (w/v), pH 8.0 and extraction solution 7 mL/g, maintaining constant buffer molarity of 0.1 M and 200 rpm. After the optimization process a 2.5 fold increase in lipase activity in the crude extract was obtained, comparing the intial value (64 U/g) with the experimental design (160 U/g), thus improving the overall productivity of the process.
authors.affiliation: Departamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Jaqueline N
authors.initials: JN
authors.lastname: Silva
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Mateus G
authors.initials: MG
authors.lastname: Godoy
authors.affiliation: Departamento de Engenharia Bioquímica, Escola de Química/Polo Xerém, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Melissa L E
authors.initials: ML
authors.lastname: Gutarra
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Denise M G
authors.initials: DM
authors.lastname: Freire
mesh_terms: D000704:Analysis of Variance; D028023:Arecaceae / Q000378:metabolism*; D045424:Complex Mixtures; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification*; D008066:Lipolysis; D010407:Penicillium / Q000201:enzymology*; D012996:Solutions; D012997:Solvents; D013696:Temperature; D013997:Time Factors; D014866:Waste Products
chemical_list: D045424:Complex Mixtures; D012996:Solutions; D012997:Solvents; D014866:Waste Products; D008049:Lipase
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