title: Direct electrochemistry of glucose oxidase and biosensing for glucose based on DNA/chitosan film.
abstract: Glucose oxidase (GOD) is widely used in the glucose biosensor industry. The amperometric biosensors based on directly electron transfer (DET) between an electrode and immobilized GOD are especially promising. In this article, GOD was immobilized with a DNA/chitosan bio-material film on GC electrode, and the DET of GOD on DNA/chitosan was studied. The cyclic voltammetric results indicated that the GOD immobilized in the DNA/chitosan film underwent DET reaction, and the cyclic voltammogram displayed a pair of well-defined redox peaks with a formal potential of -0.45 V (vs. Ag/AgCl) at pH 5.5. The response showed a surface-controlled electrode process with an electron transfer rate constant of 0.91 sec(-1) determined in the scan rate range from 10 to 100 mV/sec. The GOD immobilized in DNA/chitosan membrane retained its biocatalytic activity and stability. The immobilized GOD could electrocatalyze the reduction of dissolved oxygen and resulted in a great increase of the reduction peak current. Upon the addition of glucose, the reduction peak current decreased, which could be used for glucose detection with a sensitivity of 0.48 μA/(mmol/L), a linear range from 0.04 to 2.28 mmol/L and a detection limit of 0.04 mmol/L at a signal-to-noise ratio of 3. The sensor could exclude the interference of commonly coexisted uricacid and ascorbic acid.
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114044, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Gu
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114044, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114044, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Deng
authors.affiliation: School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114044, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Life Science & Green Chemistry, Saitama Institute of Technology, Fukaya, 369-0293, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hasebe
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D004560:Electricity; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000032:analysis*; D008297:Male
chemical_list: D002244:Carbon; D004247:DNA; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose