title: Characterisation of low molecular weight glutenin subunit genes from Pseudoroegneria spicata and Pd. strigosa.
abstract: We report the characterisation of nine novel low molecular weight glutenin subunit (LMW-GS) genes from two Pseudoroegneria species, Pd. spicata and Pd. strigosa. We found that all nine LMW-GS genes possess the same primary structure shared by other published LMW-GS. Five genes encode LMW-i type subunits, three encode LMW-m type subunits and one encodes a peptide similar to B-3 hordeins of Hordeum chilense. No LMW-s type subunit genes were found in Pseudoroegneria. One subunit, PSt24-LMW-2, contains six conserved cysteine residues, and the other eight subunits all contain eight cysteine residues. We show that one cysteine residue is located in the signal peptide of PSt24-LMW-1, suggesting a mature peptide containing only seven cysteine residues. Phylogenetic analysis indicates that the LMW-GS genes from the St genome cluster together and suggests a distant relationship with LMW-GS of the A and B genomes of wheat. Slippage/unequal crossing over and illegitimate recombination are effective mechanisms for enriching variations of seed storage proteins.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Lumin
authors.initials: L
authors.lastname: Qin
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
authors.forename: Daozheng
authors.initials: D
authors.lastname: Yang
authors.forename: Guangmin
authors.initials: G
authors.lastname: Xia
authors.forename: Shuwei
authors.initials: S
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D017343:Genes, Plant; D005983:Glutens / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D006109:Poaceae / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D021122:Protein Subunits; D005983:Glutens; C037302:glutenin
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