title: Swi2/Snf2-like protein Uls1 functions in the Sgs1-dependent pathway of maintenance of rDNA stability and alleviation of replication stress.
abstract: The Saccharomyces cerevisiae Uls1 belongs to the Swi2/Snf2 family of DNA-dependent ATPases and a new protein family of SUMO-targeted ubiquitin ligases. Here we show that Uls1 is implicated in DNA repair independently of the replication stress response pathways mediated by the endonucleases Mus81 and Yen1 and the helicases Mph1 and Srs2. Uls1 works together with Sgs1 and we demonstrate that the attenuation of replication stress-related defects in sgs1Δ by deletion of ULS1 depends on a functional of Rad51 recombinase and post-replication repair pathway mediated by Rad18 and Rad5, but not on the translesion polymerase, Rev3. The higher resistance of sgs1Δ uls1Δ mutants to genotoxic stress compared to single sgs1Δ cells is not the result of decreased formation or accelerated resolution of recombination-dependent DNA structures. Instead, deletion of ULS1 restores stability of the rDNA region in sgs1Δ cells. Our data suggest that Uls1 may contribute to genomic stability during DNA synthesis and channel the repair of replication lesions into the Sgs1-dependent pathway, with DNA translocase and SUMO binding activities of Uls1 as well as a RING domain being essential for its functions in replication stress response.
authors.affiliation: Institute of Experimental Biology, Faculty of Biological Sciences, University of Wrocław, 50-328 Wrocław, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Kramarz
authors.affiliation: Institute of Experimental Biology, Faculty of Biological Sciences, University of Wrocław, 50-328 Wrocław, Poland.
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Litwin
authors.affiliation: Institute of Experimental Biology, Faculty of Biological Sciences, University of Wrocław, 50-328 Wrocław, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Cal-Bąkowska
authors.affiliation: Fondazione IFOM, Istituto FIRC di Oncologia Molecolare, Milan 20139, Italy.
authors.forename: Barnabas
authors.initials: B
authors.lastname: Szakal
authors.affiliation: Fondazione IFOM, Istituto FIRC di Oncologia Molecolare, Milan 20139, Italy.
authors.forename: Dana
authors.initials: D
authors.lastname: Branzei
authors.affiliation: Institute of Experimental Biology, Faculty of Biological Sciences, University of Wrocław, 50-328 Wrocław, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Wysocki
authors.affiliation: Faculty of Biotechnology, University of Wrocław, 50-383 Wrocław, Poland. Electronic address: dorota.dziadkowiec@uni.wroc.pl.
authors.forename: Dorota
authors.initials: D
authors.lastname: Dziadkowiec
mesh_terms: D004249:DNA Damage; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D004275:DNA, Ribosomal / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D042822:Genomic Instability; D051135:Rad51 Recombinase / Q000235:genetics / Q000378:metabolism; D053484:RecQ Helicases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; C063607:RAD18 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C495151:RAD51 protein, S cerevisiae; D051135:Rad51 Recombinase; C076225:RAD5 protein, S cerevisiae; C090472:SGS1 protein, S cerevisiae; C506745:ULS1 protein, S cerevisiae; D004265:DNA Helicases; D053484:RecQ Helicases
keywords: Endonuclease; Helicase; Replication stress; STUbL; rDNA