title: Molecular basis of the behavior of hepatitis a virus exposed to high hydrostatic pressure.
abstract: Food-borne hepatitis A outbreaks may be prevented by subjecting foods at risk of virus contamination to moderate treatments of high hydrostatic pressure (HHP). A pretreatment promoting hepatitis A virus (HAV) capsid-folding changes enhances the virucidal effect of HHP, indicating that its efficacy depends on capsid conformation. HAV populations enriched in immature capsids (125S provirions) are more resistant to HHP, suggesting that mature capsids (150S virions) are more susceptible to this treatment. In addition, the monoclonal antibody (MAb) K24F2 epitope contained in the immunodominant site is a key factor for the resistance to HHP. Changes in capsid folding inducing a loss of recognition by MAb K24F2 render more susceptible conformations independently of the origin of such changes. Accordingly, codon usage-associated folding changes and changes stimulated by pH-dependent breathings, provided they confer a loss of recognition by MAb K24F2, induce a higher susceptibility to HHP. In conclusion, the resistance of HAV to HHP treatments may be explained by a low proportion of 150S particles combined with a good accessibility of the epitope contained in the immunodominant site close to the 5-fold axis.
authors.affiliation: Enteric Virus Laboratory, Department of Microbiology, School of Biology, University of Barcelona, Barcelona, Spain Enteric Virus Laboratory, Institute of Nutrition and Food Safety, Campus Torribera, University of Barcelona, Santa Coloma de Gramenet, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: D'Andrea
authors.affiliation: Enteric Virus Laboratory, Department of Microbiology, School of Biology, University of Barcelona, Barcelona, Spain Enteric Virus Laboratory, Institute of Nutrition and Food Safety, Campus Torribera, University of Barcelona, Santa Coloma de Gramenet, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Pérez-Rodríguez
authors.affiliation: Enteric Virus Laboratory, Department of Microbiology, School of Biology, University of Barcelona, Barcelona, Spain Enteric Virus Laboratory, Institute of Nutrition and Food Safety, Campus Torribera, University of Barcelona, Santa Coloma de Gramenet, Spain.
authors.forename: M Isabel
authors.initials: MI
authors.lastname: Costafreda
authors.affiliation: Enteric Virus Laboratory, Department of Microbiology, School of Biology, University of Barcelona, Barcelona, Spain Enteric Virus Laboratory, Institute of Nutrition and Food Safety, Campus Torribera, University of Barcelona, Santa Coloma de Gramenet, Spain.
authors.forename: Nerea
authors.initials: N
authors.lastname: Beguiristain
authors.affiliation: Enteric Virus Laboratory, Department of Microbiology, School of Biology, University of Barcelona, Barcelona, Spain Enteric Virus Laboratory, Institute of Nutrition and Food Safety, Campus Torribera, University of Barcelona, Santa Coloma de Gramenet, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Fuentes
authors.affiliation: CENTA, IRTA, Monells, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Aymerich
authors.affiliation: Enteric Virus Laboratory, Department of Microbiology, School of Biology, University of Barcelona, Barcelona, Spain Enteric Virus Laboratory, Institute of Nutrition and Food Safety, Campus Torribera, University of Barcelona, Santa Coloma de Gramenet, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Guix
authors.affiliation: Enteric Virus Laboratory, Department of Microbiology, School of Biology, University of Barcelona, Barcelona, Spain Enteric Virus Laboratory, Institute of Nutrition and Food Safety, Campus Torribera, University of Barcelona, Santa Coloma de Gramenet, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Bosch
authors.affiliation: Enteric Virus Laboratory, Department of Microbiology, School of Biology, University of Barcelona, Barcelona, Spain Enteric Virus Laboratory, Institute of Nutrition and Food Safety, Campus Torribera, University of Barcelona, Santa Coloma de Gramenet, Spain rpinto@ub.edu.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Pintó
mesh_terms: D002213:Capsid / Q000737:chemistry* / Q000276:immunology; D000939:Epitopes; D030041:Hepatitis A virus / Q000502:physiology*; D006874:Hydrostatic Pressure; D010088:Oxidoreductases / Q000276:immunology; D038301:Virus Inactivation
chemical_list: D000939:Epitopes; D010088:Oxidoreductases; C026444:1-ene-dehydrogenase
references.citation: Intervirology. 1984;22(4):218-26
references.pmid: 6096294
references.citation: J Infect Dis. 2001 Apr 15;183(8):1273-6
references.pmid: 11262211
references.citation: N Engl J Med. 1999 Feb 25;340(8):595-602
references.pmid: 10029643
references.citation: Appl Environ Microbiol. 2009 Dec;75(23):7350-5
references.pmid: 19820160
references.citation: Food Environ Virol. 2013 Mar;5(1):1-12
references.pmid: 23412716
references.citation: Appl Environ Microbiol. 2003 Mar;69(3):1840-3
references.pmid: 12620879
references.citation: J Food Prot. 2006 Oct;69(10):2454-9
references.pmid: 17066927
references.citation: J Gen Virol. 2002 Feb;83(Pt 2):359-368
references.pmid: 11807229
references.citation: Biophys J. 1999 Mar;76(3):1270-9
references.pmid: 10049311
references.citation: J Virol. 1992 Apr;66(4):2208-16
references.pmid: 1312628
references.citation: J Virol. 2004 Sep;78(18):9807-13
references.pmid: 15331714
references.citation: Appl Environ Microbiol. 2006 Jun;72(6):3846-55
references.pmid: 16751488
references.citation: MMWR Morb Mortal Wkly Rep. 1997 Apr 4;46(13):288, 295
references.pmid: 9121422
references.citation: Virus Res. 2004 Jun 15;102(2):221-4
references.pmid: 15084405
references.citation: Arch Virol. 1997;142(11):2147-60
references.pmid: 9672583
references.citation: PLoS Pathog. 2010 Mar 05;6(3):e1000797
references.pmid: 20221432
references.citation: J Gen Virol. 1981 Dec;57(Pt 2):331-41
references.pmid: 6172556
references.citation: FEBS Lett. 1998 Oct 30;438(1-2):106-10
references.pmid: 9821968
references.citation: J Food Prot. 2001 May;64(5):706-9
references.pmid: 11348005
references.citation: J Virol. 1993 Feb;67(2):1080-5
references.pmid: 7678298
references.citation: Euro Surveill. 2013 Apr 25;18(17):20467
references.pmid: 23647625
references.citation: J Biol Chem. 1999 Feb 19;274(8):4527-31
references.pmid: 9988685
references.citation: Euro Surveill. 2010 May 20;15(20):
references.pmid: 20504389
references.citation: Euro Surveill. 2013 Jul 04;18(27):
references.pmid: 23870076
references.citation: J Food Prot. 2002 Oct;65(10):1605-9
references.pmid: 12380746
references.citation: J Virol. 1985 Apr;54(1):78-85
references.pmid: 2983123
references.citation: N Engl J Med. 2005 Sep 1;353(9):890-7
references.pmid: 16135833
references.citation: Virology. 1993 Dec;197(2):616-23
references.pmid: 8249284
references.citation: J Virol. 2014 May;88(9):5029-41
references.pmid: 24554668
references.citation: J Gen Virol. 1989 Sep;70 ( Pt 9):2481-5
references.pmid: 2550575
references.citation: Int J Food Microbiol. 2012 Jan 3;152(1-2):35-9
references.pmid: 22044732
references.citation: J Virol. 2012 Aug;86(15):7887-95
references.pmid: 22593170
references.citation: J Infect Dis. 1992 Sep;166(3):518-24
references.pmid: 1323618
references.citation: Int J Food Microbiol. 2009 Mar 15;130(1):61-4
references.pmid: 19187994