title: Higher levels of circulating monocyte-platelet aggregates are correlated with viremia and increased sCD163 levels in HIV-1 infection.
abstract: Increased levels of monocyte-platelet aggregates (MPAs) are reported to be highly correlated with cardiovascular events. In this study, the MPA levels in different monocyte subsets and the associations between MPA levels, HIV-1 viremia and monocyte activation were evaluated during HIV-1 infection. The results showed that the percentages of MPAs in all three monocyte subsets were higher in HIV-1-infected subjects than in healthy controls, and were associated with the plasma viral load in the non-classical and intermediate monocyte subsets. The plasma levels of sCD14 and sCD163 were upregulated in HIV-1 infection and were positively associated with viral loads and negatively associated with CD4 counts. P-selectin glycoprotein ligand-1 (PSGL-1) was shown to be expressed at significantly lower levels on all three monocyte subsets and was negatively correlated with the sCD163 level. The MPA level was correlated with the levels of plasma sCD163 but negatively correlated with CD163 and PSGL-1 on all three monocyte subsets. An elevated immune activation status was correlated with increased MPA formation, underlying the potential interaction between monocyte activation and MPA formation. This interaction may be related to a higher thromboembolic risk in patients infected with HIV-1.Cellular & Molecular Immunology advance online publication, 11 August 2014; doi:10.1038/cmi.2014.66.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, China CDC, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Microbiology, School of Basic Medical Sciences, Peking University, Beijing, China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Duan
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, China CDC, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Beijing Chaoyang District Center for Disease Control and Prevention, Beijing, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, China CDC, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, China CDC, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Microbiology, School of Basic Medical Sciences, Peking University, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Shen
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, China CDC, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Shao
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000097:blood / Q000276:immunology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000097:blood / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology* / Q000378:metabolism; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000097:blood / Q000276:immunology; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D010974:Platelet Aggregation / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000097:blood / Q000276:immunology*; D014766:Viremia / Q000097:blood / Q000276:immunology; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D011956:Receptors, Cell Surface
references.citation: World J Gastroenterol. 2006 Feb 28;12(8):1198-204
references.pmid: 16534871
references.citation: J Infect Dis. 2011 Mar 15;203(6):780-90
references.pmid: 21252259
references.citation: J Immunol. 1999 Jul 1;163(1):367-73
references.pmid: 10384137
references.citation: J Leukoc Biol. 2011 Apr;89(4):507-15
references.pmid: 21097698
references.citation: AIDS. 2008 Mar 30;22(6):685-94
references.pmid: 18356597
references.citation: J Leukoc Biol. 2006 Mar;79(3):499-507
references.pmid: 16415171
references.citation: Arterioscler Thromb Vasc Biol. 2000 Dec;20(12):2702-8
references.pmid: 11116075
references.citation: J Infect Dis. 2011 Jul 1;204(1):154-63
references.pmid: 21628670
references.citation: J Immunol. 2000 Sep 1;165(5):2764-72
references.pmid: 10946308
references.citation: Nephrol Dial Transplant. 2003 Oct;18(10):2088-96
references.pmid: 13679485
references.citation: Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):8767-71
references.pmid: 7522321
references.citation: J Am Coll Cardiol. 2001 Oct;38(4):1002-6
references.pmid: 11583872
references.citation: BMC Infect Dis. 2013 May 21;13:230
references.pmid: 23692821
references.citation: Clin Infect Dis. 2014 Feb;58(4):588-95
references.pmid: 24253250
references.citation: J Virol Methods. 2012 May;181(2):170-6
references.pmid: 22387340
references.citation: J Infect Dis. 2011 Oct 15;204(8):1227-36
references.pmid: 21917896
references.citation: Immunity. 2010 Sep 24;33(3):289-91
references.pmid: 20870168
references.citation: Cytometry B Clin Cytom. 2005 Jan;63(1):16-22
references.pmid: 15624200
references.citation: J Am Coll Cardiol. 1998 Feb;31(2):352-8
references.pmid: 9462579
references.citation: J Am Coll Cardiol. 2012 Oct 16;60(16):1512-20
references.pmid: 22999728
references.citation: Thromb Haemost. 2009 Nov;102(5):916-24
references.pmid: 19888530
references.citation: J Clin Invest. 1995 May;95(5):2297-303
references.pmid: 7537762
references.citation: J Acquir Immune Defic Syndr. 2009 Dec;52(5):553-9
references.pmid: 19950429
references.citation: Blood. 2012 Nov 29;120(23):4599-608
references.pmid: 23065151
references.citation: Circulation. 2001 Sep 25;104(13):1533-7
references.pmid: 11571248
references.citation: Curr Opin HIV AIDS. 2013 Mar;8(2):117-24
references.pmid: 23274365
references.citation: PLoS One. 2013 May 28;8(5):e63804
references.pmid: 23724003
references.citation: HIV Med. 2011 Sep;12(8):510-5
references.pmid: 21375686
references.citation: Arterioscler Thromb Vasc Biol. 2008 Aug;28(8):1491-8
references.pmid: 18497306
references.citation: Circulation. 2002 May 7;105(18):2166-71
references.pmid: 11994250
references.citation: Immunity. 2010 Sep 24;33(3):375-86
references.pmid: 20832340
references.citation: J Mol Biol. 2014 Mar 20;426(6):1133-47
references.pmid: 24262390
references.citation: Blood. 2010 Oct 21;116(16):e74-80
references.pmid: 20628149
references.citation: AIDS. 2008 Mar 12;22(5):655-8
references.pmid: 18317010
references.citation: PLoS Pathog. 2013;9(6):e1003453
references.pmid: 23818854
references.citation: Blood. 2012 Aug 16;120(7):1357-66
references.pmid: 22653975
references.citation: Am J Pathol. 2008 Apr;172(4):1141-52
references.pmid: 18349125
references.citation: Metab Syndr Relat Disord. 2010 Jun;8(3):279-86
references.pmid: 20235745
references.citation: J Infect Dis. 2014 Mar;209(6):931-9
references.pmid: 24185941
references.citation: AIDS Patient Care STDS. 2007 Jul;21(7):452-7
references.pmid: 17651026
references.citation: Atherosclerosis. 2006 Feb;184(2):342-7
references.pmid: 15979079
references.citation: Arthritis Res Ther. 2009;11(5):R156
references.pmid: 19832990
references.citation: J Infect Dis. 2013 Sep;208(6):874-83
references.pmid: 23852120
references.citation: Curr Opin Virol. 2011 Dec;1(6):447-54
references.pmid: 22440908
references.citation: Scand J Clin Lab Invest. 2012 Feb;72(1):1-13
references.pmid: 22060747