title: Molecular characterization and B cell membrane expression analysis of Fc fragment gene of goose IgY.
abstract: A novel goose immunoglobulin υ chain (Igυ) Fc fragment gene was cloned from splenic tissue mRNA using RT-PCR. Deduced amino acid sequence data from different vertebrates revealed high similarity to IgY-Fc fragments of duck (91%) and chicken (64%). Molecular characterization showed that the goose IgY-Fc fragment was consistent with the definition of immunoglobulin, and had the same antigenicity to natural IgY. Flow cytometry and laser scanning confocal microscopy showed that the polyclonal antibody against GoυFc reacted with the membrane surface of B lymphocytes in peripheral blood, which indicates that IgY was expressed on the surface of B cells. Analyses of the gene sequence of the goose IgY-Fc fragment and expression of B cell membrane may provide insight into the evolution of the Ig heavy chain gene family and benefit future studies on the avian immune system.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: gaomingchun@163.com.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Haili
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin 150030, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xiuxin
authors.initials: X
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism*; D002645:Chickens / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004372:Ducks / Q000235:genetics; D005777:Geese / Q000235:genetics*; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007136:Immunoglobulins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D018009:Receptors, Glycine / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; C016512:IgY; D007141:Immunoglobulin Fc Fragments; D007136:Immunoglobulins; D018009:Receptors, Glycine
keywords: B cell; Goose; IgY-Fc fragment; Membrane expression; Sequence analysis