title: Different stressors induce differential responses of the CRH-stress system in the gilthead sea bream (Sparus aurata).
abstract: The hypothalamus-pituitary-interrenal (HPI) axis, involved in the regulation of the neuroendocrine stress responses, presents important players such as corticotropin-releasing hormone (CRH, generally considered as the initiator of this pathway) and CRH-binding protein (CRH-BP, considered as an antagonist of CRH function). CRH and CRH-BP full-length cDNA sequences were obtained from Sparus aurata by screening a brain cDNA library, and their phylogenetic analysis as well as their roles during acute and chronic stress responses were assessed. mRNA expression levels and plasma cortisol concentrations were measured by RT qPCR and ELISA, respectively, in S. aurata juveniles submitted to: i) different environmental salinities in a short-time course response; and ii) food deprivation during 21 days. In addition, osmoregulatory and metabolic parameters in plasma corroborated a clear reorganization depending on the stress source/period. Salinity transfer induced stress as indicated by enhanced plasma cortisol levels, as well as by up-regulated CRH and down-regulated CRH-BP expression values. On the other hand, food deprivation did not affect both expression levels, although plasma cortisol concentrations were enhanced. These results suggest that different stressors are handled through different stress pathways in S. aurata.
authors.affiliation: Department of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cádiz, 11510 Puerto Real (Cádiz), Spain; Department of Marine Biology and Aquaculture, Instituto de Ciencias Marinas de Andalucía (CSIC), Apartado Oficial, 11510 Puerto Real (Cádiz), Spain. Electronic address: juanantonio.sitcha@icman.csic.es.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Martos-Sitcha
authors.affiliation: Department of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cádiz, 11510 Puerto Real (Cádiz), Spain; Department of Organismal Animal Physiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University Nijmegen, Heyendaalseweg 135 6525 AJ, Nijmegen, The Netherlands.
authors.forename: Yvette S
authors.initials: YS
authors.lastname: Wunderink
authors.affiliation: Department of Organismal Animal Physiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University Nijmegen, Heyendaalseweg 135 6525 AJ, Nijmegen, The Netherlands.
authors.forename: Justin
authors.initials: J
authors.lastname: Straatjes
authors.affiliation: Department of Marine Biology and Aquaculture, Instituto de Ciencias Marinas de Andalucía (CSIC), Apartado Oficial, 11510 Puerto Real (Cádiz), Spain.
authors.forename: Arleta K
authors.initials: AK
authors.lastname: Skrzynska
authors.affiliation: Department of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cádiz, 11510 Puerto Real (Cádiz), Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Mancera
authors.affiliation: Department of Marine Biology and Aquaculture, Instituto de Ciencias Marinas de Andalucía (CSIC), Apartado Oficial, 11510 Puerto Real (Cádiz), Spain.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Martínez-Rodríguez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology; D003346:Corticotropin-Releasing Hormone / Q000097:blood* / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D015723:Gene Library; D006854:Hydrocortisone / Q000097:blood; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D054712:Salinity; D021541:Sea Bream / Q000097:blood / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone; D006854:Hydrocortisone
keywords: CRH; CRH-binding protein; Cortisol; Environmental salinity; Food deprivation; Sparus aurata