title: Crystallographic characterization of the C-terminal coiled-coil region of mouse Bicaudal-D1 (BICD1).
abstract: Bicaudal-D1 (BICD1) is an α-helical coiled-coil protein which is evolutionarily conserved from Drosophila to mammals and facilitates the attachment of specific cargo factors to the dynein motor complex. The C-terminal coiled-coil region (CC3) of BICD1 plays an important role in sorting cargo, linking proteins such as the small GTPase Rab6 and the nuclear pore complex component Ran-binding protein 2 (RanBP2) to the dynein motor complex. This report describes the crystallization and X-ray data collection of the BICD1 CC3 region, as well as the preparation of the complex of BICD1 CC3 with a constitutively active mutant of Rab6. The crystals of the BICD1 CC3 region belonged to space group C2, with unit-cell parameters a = 59.0, b = 36.8, c = 104.3 Å, α = γ = 90, β = 99.8°. The X-ray diffraction data set was collected to 1.50 Å resolution.
authors.affiliation: Faculty of Gunma University, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Terawaki
authors.affiliation: Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ootsuka
authors.affiliation: Department of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
authors.affiliation: Faculty of Gunma University, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Wakamatsu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry*; D000818:Animals; D001483:Base Sequence; D018360:Crystallography, X-Ray; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D017931:DNA Primers; D051379:Mice; D011487:Protein Conformation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D017931:DNA Primers; C000591599:Bicd1 protein, mouse
keywords: BICD1; Rab6; retrograde transport
references.citation: Am J Hum Genet. 2013 Jun 6;92(6):955-64
references.pmid: 23664119
references.citation: Methods Enzymol. 1997;276:523-30
references.pmid: 9048379
references.citation: EMBO J. 2006 Dec 13;25(24):5670-82
references.pmid: 17139249
references.citation: Nat Cell Biol. 1999 Nov;1(7):444-9
references.pmid: 10559989
references.citation: Am J Hum Genet. 2013 Jun 6;92(6):946-54
references.pmid: 23664116
references.citation: Am J Hum Genet. 2013 Jun 6;92(6):965-73
references.pmid: 23664120
references.citation: Genes Dev. 1989 Dec;3(12A):1957-68
references.pmid: 2576013
references.citation: EMBO J. 2003 Nov 17;22(22):6004-15
references.pmid: 14609947
references.citation: Neuron. 2010 Nov 18;68(4):610-38
references.pmid: 21092854
references.citation: Mol Biol Cell. 2012 Nov;23(21):4226-41
references.pmid: 22956769
references.citation: Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):30-42
references.pmid: 15299723
references.citation: Methods Mol Biol. 2007;364:215-30
references.pmid: 17172768
references.citation: Development. 1993 Aug;118(4):1169-80
references.pmid: 8269846
references.citation: Biochem Soc Trans. 2009 Oct;37(Pt 5):1066-71
references.pmid: 19754453
references.citation: Nature. 2001 Dec 6;414(6864):611-6
references.pmid: 11740552
references.citation: Nat Cell Biol. 2004 May;6(5):427-35
references.pmid: 15077115
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707
references.citation: Development. 1996 Nov;122(11):3577-86
references.pmid: 8951073
references.citation: PLoS Biol. 2010 Apr 06;8(4):e1000350
references.pmid: 20386726
references.citation: J Mol Biol. 1993 Jan 5;229(1):105-24
references.pmid: 7678431
references.citation: Genetics. 1986 Jul;113(3):665-78
references.pmid: 3089869
references.citation: PLoS One. 2008;3(11):e3763
references.pmid: 19018277
references.citation: EMBO J. 2001 Aug 1;20(15):4041-54
references.pmid: 11483508
references.citation: Curr Biol. 2006 Jul 25;16(14):1447-52
references.pmid: 16860745
references.citation: Exp Cell Res. 2007 Oct 1;313(16):3408-20
references.pmid: 17707369
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: Cell. 1989 Dec 1;59(5):881-92
references.pmid: 2590944
references.citation: Genomics. 1997 Nov 1;45(3):601-6
references.pmid: 9367685
references.citation: Genetics. 1986 Apr;112(4):803-22
references.pmid: 3082713
references.citation: Nat Cell Biol. 2002 Dec;4(12):986-92
references.pmid: 12447383
references.citation: Cell. 2005 Jul 15;122(1):97-106
references.pmid: 16009136