title: [Determination of four kinds of purines in deer fetus soft capsule by HPLC].
abstract: OBJECTIVE
  To establish an HPLC method for simultaneous determination of four kinds of purines in deer fetus soft capsule.
  
  
  METHODS
  Four kinds of purines were detected and determined by HPLC. The mobile phase was 0.02 mol/L KH2PO4 (containing 1 mmol/L heptane sulfonic acid sodium, pH = 3.8)-methanol = 97:3. Detection wavelength was 254 nm and flow rate was 1.5 ml min. The linear relationship of four kinds of purines was as follows: hypoxanthine: Y1 = 83695X1 + 355 (r1 = 0.9998), with the linear range 0.040-0.667 mg/mL; xanthine: Y2 = 50638X2 + 39 (r2 = 0.9989), with the linear range 0.008-0.119 mg/mL; guanine: Y3 = 30269X3-9562 (r3 = 0.9924), with the linear range 0.018 - 0.279 mg/mL; adenine: Y4 = 38975X4-8671 (r4 = 0.9989), with the linear range 0.027-0.399 mg/mL The average sample recovery rate of hypoxanthine, xanthine, guanine and adenine were 98.1%, 98.6%, 98.0% and 97.5%, with RSD 1.0%, 0.4%, 0.8% and 0.6%, respectively.
  
  
  RESULTS
  The content of hypoxanthine, xanthine, guanine and adenine in 3 lots of deer fetus soft capsule were 116.5-132.0 microg/capsule, 21.2-23.0 microg/capsule, 48.6-54.3 microg/capsule and 68.9-75.2 microg/capsule, respectively.
  
  
  CONCLUSIONS
  This method is simple,accurate and reproducible, which provides a basis for quality control of purines in deer fetus soft capsule.
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Song
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
mesh_terms: D000225:Adenine / Q000032:analysis; D000818:Animals; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003670:Deer; D005333:Fetus; D006147:Guanine / Q000032:analysis; D019271:Hypoxanthine / Q000032:analysis; D008419:Materia Medica / Q000737:chemistry*; D011687:Purines / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D019820:Xanthine / Q000032:analysis
chemical_list: D002214:Capsules; D008419:Materia Medica; D011687:Purines; D019820:Xanthine; D019271:Hypoxanthine; D006147:Guanine; D000225:Adenine; C030985:purine