title: In vitro free radical scavenging and DNA damage protective property of Coriandrum sativum L. leaves extract.
abstract: Coriandrum sativum L. (coriander), an everyday spice in the Indian kitchen is known to add flavor to the cuisine. It is an annual herb belonging to the Apiaceae (Umbellifera) family. The hydro-alcohol extract of Coriandrum sativum L. at the dose of 1 mg/ml was subjected to a series of in vitro assays viz. 2, 2'- diphenyl-1-picrylhydrazyl, lipid peroxidation by thiobarbituric acid, reducing power and nitric oxide (NO) radical scavenging in order to study its antioxidant efficacy in detail. The amount of flavonoids in 70% ethanol extract was found to be 44.5 μg and that of the total phenols was 133.74 μg gallic acid equivalents per mg extract. The extracts of the leaves showed metal chelating power, with IC50 values, 368.12 μg/ml where as that of standard EDTA was 26.7 μg/ml. The IC50 values for 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid radical scavenging was 222 μg/ml where as that of standard ascorbic acid was 22.6 μg/ml. The NO scavenging activity of the extract of the leaves showed IC50 value of 815.6 μg/ml; at the same time the standard BHA had 49.1 μg/ml. All the plant extracts provided DNA damage protection; however, the protection provided at the dose of 8 μg/ml was comparable to that of standard gallic acid. The Coriandrum sativum leaf extract was able to prevent in vitro lipid peroxidation with IC50 values; 589.6 μg/ml where as that of standard BHA was 16.3 μg/ml. Our results also showed significant ferric reducing power indicating the hydrogen donating ability of the extract. This study indicated the potential of the leaf extract as a source of natural antioxidants or nutraceuticals that could be of use in food industry with potential application to reduce oxidative stress in living system.
authors.affiliation: Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Mysore, 570011 India.
authors.forename: S N
authors.initials: SN
authors.lastname: Harsha
authors.affiliation: Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Mysore, 570011 India.
authors.forename: K R
authors.initials: KR
authors.lastname: Anilakumar
keywords: ABTS; Antioxidant; Coriandrum sativum; DNA damage; DPPH; Reactive oxygen species
references.citation: J Ethnopharmacol. 2000 Aug;71(3):457-63
references.pmid: 10940583
references.citation: Plant Foods Hum Nutr. 1997;51(2):167-72
references.pmid: 9527351
references.citation: Free Radic Biol Med. 1999 Feb;26(3-4):463-71
references.pmid: 9895239
references.citation: Anal Biochem. 1997 Feb 15;245(2):161-6
references.pmid: 9056207
references.citation: Fitoterapia. 2001 Mar;72(3):272-7
references.pmid: 11295303
references.citation: Free Radic Biol Med. 1999 May;26(9-10):1231-7
references.pmid: 10381194
references.citation: J Ethnopharmacol. 2005 Jan 4;96(1-2):145-50
references.pmid: 15588663
references.citation: Chem Pharm Bull (Tokyo). 1988 Jun;36(6):2090-7
references.pmid: 3240445
references.citation: J Agric Food Chem. 1999 Oct;47(10):3954-62
references.pmid: 10552749
references.citation: Food Chem Toxicol. 2009 Feb;47(2):279-82
references.pmid: 18940228
references.citation: Arch Biochem Biophys. 1994 Nov 15;315(1):161-9
references.pmid: 7979394
references.citation: Biochim Biophys Acta. 1983 Aug 23;759(1-2):38-41
references.pmid: 6192847
references.citation: Biochem Biophys Res Commun. 1994 Jun 15;201(2):748-55
references.pmid: 8003011
references.citation: Pharmacol Res. 2001 Dec;44(6):491-4
references.pmid: 11735355
references.citation: Carcinogenesis. 1995 Sep;16(9):2045-50
references.pmid: 7554052
references.citation: J Ethnopharmacol. 2005 Jan 15;96(3):365-70
references.pmid: 15619553
references.citation: Diabetologia. 1990 Aug;33(8):462-4
references.pmid: 2210118
references.citation: Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):7915-22
references.pmid: 8367443
references.citation: Anal Biochem. 1996 Jul 15;239(1):70-6
references.pmid: 8660627
references.citation: J Diet Suppl. 2010 Mar;7(1):9-20
references.pmid: 22435570
references.citation: Free Radic Res. 1997 May;26(5):469-78
references.pmid: 9179593
references.citation: Nephrol Dial Transplant. 2001 Aug;16(8):1577-82
references.pmid: 11477158
references.citation: Nutr Rev. 1997 Jan;55(1 Pt 2):S44-9; discussion S49-52
references.pmid: 9155225
references.citation: Genes Nutr. 2008 Dec;3(3-4):115-26
references.pmid: 18937002
references.citation: Anal Biochem. 1979 Jun;95(2):351-8
references.pmid: 36810
references.citation: Crit Rev Food Sci Nutr. 1992;32(1):67-103
references.pmid: 1290586
references.citation: Planta Med. 1984 Dec;50(6):473-7
references.pmid: 6531408
references.citation: Br J Nutr. 1999 Mar;81(3):203-9
references.pmid: 10434846
references.citation: Biochim Biophys Acta. 1999 Nov 16;1472(3):643-50
references.pmid: 10564778
references.citation: J Agric Food Chem. 2004 Aug 11;52(16):5032-9
references.pmid: 15291471
references.citation: Clin Chem. 2004 Sep;50(9):1668-70
references.pmid: 15331502
references.citation: Genes Nutr. 2009 Dec;4(4):243-50
references.pmid: 19685255
references.citation: Lancet. 1984 Jun 23;1(8391):1396-7
references.pmid: 6145845
references.citation: Phytother Res. 2003 Sep;17(8):870-5
references.pmid: 13680815