title: The TLR3, PI3K, survivin, FasL, and Fas genes as major risk factors of occurrence and development of cervical cancer disease.
abstract: To investigate the role of TLR3/PI3K signals in the occurrence and development of cervical cancer disease, TLR3-siRNA was used to block key signaling pathways involved in cervical cancer metastasis that are pivotal to metastatic tumor cells but not to normal cells under ordinary physiologic conditions. Results show that tumor U14 cell growth, migration and invasion in TLR3-siRNA treatment group were significantly decreased. Through LY294002 suppressing targeted gene, the LY294002 treatment specifically and significantly knocked down the expressions of tumor TLR3 and PI3K proteins in cervical cancer mice. Furthermore, expressions of tumor Survivin and FasL proteins were markedly suppressed, whereas expressions of Fas protein were upregulated in LY294002 treatment group mice. LY294002 treatment suppressed tumor growth and increased the thymus and spleen indeces and survival days of cervical cancer mice. This study demonstrates that TLR3-siRNA and LY294002 treatments can markedly suppress cervical cancer cell invasion and tumor growth and increase survival life by silencing targeted genes.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Hui Na
authors.initials: HN
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: huirongshizzdr@126.com.
authors.forename: Hui Rong
authors.initials: HR
authors.lastname: Shi
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Xian Lan
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Rui Tao
authors.initials: RT
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, Zhengzhou 450003, China.
authors.forename: Guang Zhi
authors.initials: GZ
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, Zhengzhou 450003, China.
authors.forename: Ju Xin
authors.initials: JX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002867:Chromones / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009025:Morpholines / Q000494:pharmacology; D009929:Organ Size / Q000187:drug effects; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D000077022:Survivin; D013950:Thymus Gland / Q000187:drug effects / Q000473:pathology; D051196:Toll-Like Receptor 3 / Q000235:genetics / Q000378:metabolism*; D047368:Tumor Burden / Q000187:drug effects / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000517:prevention & control; D019014:fas Receptor
chemical_list: D002867:Chromones; D004791:Enzyme Inhibitors; D053222:Fas Ligand Protein; D051034:Inhibitor of Apoptosis Proteins; D009025:Morpholines; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D012097:Repressor Proteins; D000077022:Survivin; D051196:Toll-Like Receptor 3; D019014:fas Receptor; C117749:Birc5 protein, mouse; C491899:Fas protein, mouse; C519703:TLR3 protein, mouse; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
keywords: Cervical cancer; Mice; Survivin; TLR3/PI3K signals