title: Isolation of endothelial cells from mouse lung.
abstract: The isolation of endothelial cells (ECs) from knockout and transgenic mouse lines provides the opportunity to study the endothelial-specific activities of a targeted molecule. As a means of pursuing these types of investigations, the protocols described in this unit provide a reliable method for isolating lung microvascular ECs from mouse neonatal pups that can be serially passaged. These protocols are useful in settings where mouse age is irrelevant and a pure population of pulmonary vascular ECs, uncontaminated by other cells, is needed. When a specific source of ECs is not required, these procedures also represent a reliable means of obtaining murine ECs in general.
authors.affiliation: Pulmonary, Allergy and Critical Care Division, Department of Medicine, Perelman School of School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
authors.forename: Gaoyuan
authors.initials: G
authors.lastname: Cao
authors.forename: Valsamma
authors.initials: V
authors.lastname: Abraham
authors.forename: Horace M
authors.initials: HM
authors.lastname: DeLisser
mesh_terms: D000818:Animals; D004727:Endothelium / Q000166:cytology*; D008168:Lung / Q000166:cytology*; D051379:Mice
keywords: endothelial cells; lung cells; microvascular cells