title: RNA-seq-mediated transcriptome analysis of actively growing and winter dormant shoots identifies non-deciduous habit of evergreen tree tea during winters.
abstract: Tea [Camellia sinensis (L.) O. Kuntze] is a perennial tree which undergoes winter dormancy and unlike deciduous trees, the species does not shed its leaves during winters. The present work dissected the molecular processes operating in the leaves during the period of active growth and winter dormancy through transcriptome analysis to understand a long-standing question: why should tea be a non-deciduous species? Analyses of 24,700 unigenes obtained from 57,767 primarily assembled transcripts showed (i) operation of mechanisms of winter tolerance, (ii) down-regulation of genes involved in growth, development, protein synthesis and cell division, and (iii) inhibition of leaf abscission due to modulation of senescence related processes during winter dormancy in tea. These senescence related processes exhibited modulation to favour leaf abscission (i) in deciduous Populus tremula during winters, and (ii) also in tea but under osmotic stress during which leaves also abscise. These results validated the relevance of the identified senescence related processes for leaf abscission and suggested their operation when in need in tea.
authors.affiliation: 1] Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur-176061, Himachal Pradesh, India [2] [3].
authors.forename: Asosii
authors.initials: A
authors.lastname: Paul
authors.affiliation: 1] Studio of Computational Biology &Bioinformatics, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur-176061, Himachal Pradesh, India [2].
authors.forename: Ashwani
authors.initials: A
authors.lastname: Jha
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur-176061, Himachal Pradesh, India.
authors.forename: Shruti
authors.initials: S
authors.lastname: Bhardwaj
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur-176061, Himachal Pradesh, India.
authors.forename: Sewa
authors.initials: S
authors.lastname: Singh
authors.affiliation: Studio of Computational Biology &Bioinformatics, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur-176061, Himachal Pradesh, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Shankar
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur-176061, Himachal Pradesh, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000375:Aging / Q000235:genetics; D028241:Camellia sinensis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D002455:Cell Division; D003080:Cold Temperature; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014176:Protein Biosynthesis; D012621:Seasons; D013662:Tea / Q000737:chemistry; D059467:Transcriptome; D014197:Trees / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D013662:Tea
references.citation: Plant Physiol. 2009 May;150(1):365-77
references.pmid: 19261734
references.citation: Trends Plant Sci. 2000 Dec;5(12):523-30
references.pmid: 11120474
references.citation: BMC Genomics. 2012 Mar 31;13:126
references.pmid: 22462805
references.citation: Plant Cell Physiol. 2011 Sep;52(9):1583-602
references.pmid: 21828102
references.citation: J Exp Bot. 2011 Nov;62(15):5607-21
references.pmid: 21862479
references.citation: Plant Cell Rep. 2012 Jan;31(1):27-34
references.pmid: 21850593
references.citation: Mol Biol Rep. 2009 May;36(5):921-7
references.pmid: 18454353
references.citation: BMC Genomics. 2008 Nov 12;9:536
references.pmid: 19014493
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: Plant Mol Biol. 2006 May;61(1-2):329-44
references.pmid: 16786310
references.citation: Planta. 2011 May;233(5):971-83
references.pmid: 21274560
references.citation: J Exp Bot. 2007;58(5):1035-45
references.pmid: 17244630
references.citation: Development. 2005 Oct;132(20):4563-74
references.pmid: 16176952
references.citation: New Phytol. 2006;170(4):723-38
references.pmid: 16684234
references.citation: Plant Physiol. 2003 Feb;131(2):430-42
references.pmid: 12586868
references.citation: Tree Physiol. 2007 Sep;27(9):1253-9
references.pmid: 17545125
references.citation: BMC Bioinformatics. 2008 Apr 09;9:180
references.pmid: 18400082
references.citation: Nucleic Acids Res. 2011 Jan;39(Database issue):D225-9
references.pmid: 21109532
references.citation: Biochemistry. 2001 Jan 16;40(2):569-76
references.pmid: 11148052
references.citation: Biochem Biophys Res Commun. 2005 Apr 15;329(3):831-8
references.pmid: 15752731
references.citation: Development. 2006 May;133(10):1857-69
references.pmid: 16651539
references.citation: Mol Biol Rep. 2013 May;40(5):3859-63
references.pmid: 23275208
references.citation: Biotechnol Biofuels. 2012 Dec 21;5(1):89
references.pmid: 23259593
references.citation: Mol Biol Rep. 2012 Apr;39(4):3485-90
references.pmid: 21725638
references.citation: PLoS Comput Biol. 2012;8(4):e1002486
references.pmid: 22570599
references.citation: BMC Plant Biol. 2009 Nov 26;9:139
references.pmid: 19941659
references.citation: J Exp Bot. 2010 Mar;61(5):1419-30
references.pmid: 20164142
references.citation: Mol Biotechnol. 2012 Sep;52(1):82-8
references.pmid: 22144070
references.citation: Plant J. 2007 May;50(4):557-73
references.pmid: 17419838
references.citation: Genome Res. 2008 Sep;18(9):1509-17
references.pmid: 18550803
references.citation: Science. 1995 Dec 22;270(5244):1986-8
references.pmid: 8592746
references.citation: Plant Physiol. 2001 Nov;127(3):937-48
references.pmid: 11706176
references.citation: Bioinformatics. 2010 Jan 1;26(1):139-40
references.pmid: 19910308
references.citation: Genome Res. 2010 Feb;20(2):265-72
references.pmid: 20019144
references.citation: Plant Physiol. 2013 Jun;162(2):991-1005
references.pmid: 23629833
references.citation: Bioinformatics. 2005 Sep 15;21(18):3674-6
references.pmid: 16081474
references.citation: Nature. 2004 Apr 22;428(6985):821-7
references.pmid: 15103368
references.citation: Funct Integr Genomics. 2011 Dec;11(4):659-64
references.pmid: 21755357
references.citation: Genome Biol. 2004;5(4):R24
references.pmid: 15059257
references.citation: Mol Biol Rep. 2009 Mar;36(3):537-42
references.pmid: 18224457
references.citation: Plant Cell. 2007 Aug;19(8):2370-90
references.pmid: 17693531
references.citation: Plant Physiol. 1964 Nov;39(6):963-9
references.pmid: 16656043
references.citation: Plant Physiol Biochem. 2005 Apr;43(4):383-8
references.pmid: 15907690
references.citation: Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4788-93
references.pmid: 11287667
references.citation: Nucleic Acids Res. 2010 Jan;38(Database issue):D822-7
references.pmid: 19858103
references.citation: Funct Integr Genomics. 2012 Aug;12(3):565-71
references.pmid: 22543414
references.citation: Nucleic Acids Res. 2010 Jul;38(Web Server issue):W64-70
references.pmid: 20435677