title: Screening essential genes of Mycobacterium tuberculosis with the pathway enrichment method.
abstract: The number of effective drugs for the prevention and control of tuberculosis is very limited. Therefore, high-throughput screening for Mycobacterium tuberculosis drug targets is critical. In addition, determining the essential gene cluster is important for both understanding a survival mechanism and finding novel molecular targets for anti-tuberculosis drugs. In this study, we applied the pathway enrichment method to perform high throughput screening of genes encoding key molecules for potential drug targets for M. tuberculosis. Our results indicated 122 genes that existed in more than three pathways, while four existed in 11 pathways. We predicted 55 genes that are potentially essential genes. Four of them, namely, Rv0363c, Rv0408, Rv0409 and Rv0794c, had the highest probability to be essential genes, and thus further experimental validation is warranted.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, 130021, Jilin, China, xuguangyu2005@163.com.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Xu
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Ni
authors.forename: Yue
authors.initials: Y
authors.lastname: Shi
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Sun
authors.forename: Huaidong
authors.initials: H
authors.lastname: Wang
authors.forename: Chengguo
authors.initials: C
authors.lastname: Wei
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Li
mesh_terms: D000995:Antitubercular Agents; D016503:Drug Delivery Systems / Q000379:methods*; D020043:Genes, Essential / Q000235:genetics*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics; D015999:Multivariate Analysis; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D014376:Tuberculosis / Q000188:drug therapy*
chemical_list: D000995:Antitubercular Agents
references.citation: Science. 2001 Sep 21;293(5538):2266-9
references.pmid: 11567142
references.citation: Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):425-30
references.pmid: 16407165
references.citation: Curr Opin Chem Biol. 1997 Dec;1(4):579-88
references.pmid: 9667898
references.citation: J Pharm Pharm Sci. 2011;14(2):148-61
references.pmid: 21733406
references.citation: PLoS Pathog. 2007 Mar;3(3):e24
references.pmid: 17352532
references.citation: Annu Rev Microbiol. 1996;50:791-824
references.pmid: 8905099
references.citation: Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4678-83
references.pmid: 12682299
references.citation: Nat Rev Microbiol. 2003 Nov;1(2):127-36
references.pmid: 15035042
references.citation: Curr Pharm Des. 2002;8(13):1155-72
references.pmid: 12052225
references.citation: J Infect Dis. 2010 Jun 1;201(11):1743-52
references.pmid: 20394526
references.citation: AIDS. 2007 Jan 11;21(2):248-50
references.pmid: 17197821
references.citation: Curr Med Chem. 2008;15(19):1956-67
references.pmid: 18691051
references.citation: Asian Pac J Cancer Prev. 2011;12(8):2055-8
references.pmid: 22292650
references.citation: Mol Microbiol. 2002 Mar;43(6):1387-400
references.pmid: 11952893
references.citation: Curr Opin Biotechnol. 2006 Oct;17(5):448-56
references.pmid: 16978855
references.citation: J Proteome Res. 2012 Sep 7;11(9):4682-92
references.pmid: 22808930
references.citation: Biochem Biophys Res Commun. 2004 Sep 10;322(1):347-54
references.pmid: 15313213
references.citation: Microbiology (Reading). 2012 Feb;158(Pt 2):319-327
references.pmid: 22075031
references.citation: Drug Discov Today. 2008 Dec;13(23-24):1090-8
references.pmid: 18840542
references.citation: FEBS J. 2010 Jun;277(11):2416-27
references.pmid: 20553485
references.citation: Microbiology (Reading). 2001 Apr;147(Pt 4):1017-1024
references.pmid: 11283297
references.citation: Ann Saudi Med. 2004 Mar-Apr;24(2):102-5
references.pmid: 15323270
references.citation: Nature. 2002 Jul 25;418(6896):387-91
references.pmid: 12140549
references.citation: J Bacteriol. 2011 Apr;193(8):1981-90
references.pmid: 21335456
references.citation: Curr Opin Pharmacol. 2006 Oct;6(5):459-67
references.pmid: 16904376