title: [Simultaneous determination of 4 alkaloids and a flavonoid in Picrasmae Ramulus et Folium by RP-HPLC].
abstract: A RP-HPLC method was developed to evaluate the quality of Picrasmae Ramulus et Folium by simultaneous determination of five constituents including 1-hydroxymethyl-beta-carboline (1), 1-methoxicabony-beta-carboline (2), 4-methoxy-5-hydroxy-canthin-6-one (3), 4, 5-dimethoxy-canthin-6-one (4) and maackiain (5) in Picrasmae Ramulus et Folium. The samples were separated on a Kromasil RP-C18 (4.6 mm x 250 mm, 5 microm) column eluted with acetonitrile and 0.1% phosphoric acid as mobile phases in gradient mode. The detection wavelength was set at 254 nm. The calibration curves and linearity of the above five standards were determined as (1) Y = 6 525.6X + 37.25 (0.009-1.780 microg, r = 0.996 8), (2) Y = 3 662.3X + 41.55 (0.005-0.920 microg, r = 0.999 5), (3) Y = 3763.1X + 146.87 (0.015-3.060 microg, r = 0.999 0), (4) Y = 2 174.1X + 21.52 (0.003-0.620 microg, r = 0.999 5), and (5) Y = 276.25X + 7.65 (0.010-1.960 microg, r = 0.998 9), respectively. The method is simple and repeatable, and can be used for the quality assessment of Picrasmae Ramulus et Folium.
authors.forename: Chong
authors.initials: C
authors.lastname: Wu
authors.forename: Gui-Hua
authors.initials: GH
authors.lastname: Deng
authors.forename: Chao-Zhan
authors.initials: CZ
authors.lastname: Lin
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Zhu
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002138:Calibration; D002243:Carbolines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D026121:Indole Alkaloids / Q000032:analysis; D032270:Picrasma / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D036343:Pterocarpans / Q000032:analysis; D015203:Reproducibility of Results; D000094162:Phytoalexins
chemical_list: D000470:Alkaloids; D002243:Carbolines; D005419:Flavonoids; D026121:Indole Alkaloids; D036343:Pterocarpans; C453379:canthin-6-one; C001449:inermin; D000094162:Phytoalexins