title: Bioactivity and structural properties of chimeric analogs of the starfish SALMFamide neuropeptides S1 and S2.
abstract: The starfish SALMFamide neuropeptides S1 (GFNSALMFamide) and S2 (SGPYSFNSGLTFamide) are the prototypical members of a family of neuropeptides that act as muscle relaxants in echinoderms. Comparison of the bioactivity of S1 and S2 as muscle relaxants has revealed that S2 is ten times more potent than S1. Here we investigated a structural basis for this difference in potency by comparing the bioactivity and solution conformations (using NMR and CD spectroscopy) of S1 and S2 with three chimeric analogs of these peptides. A peptide comprising S1 with the addition of S2's N-terminal tetrapeptide (Long S1 or LS1; SGPYGFNSALMFamide) was not significantly different to S1 in its bioactivity and did not exhibit concentration-dependent structuring seen with S2. An analog of S1 with its penultimate residue substituted from S2 (S1(T); GFNSALTFamide) exhibited S1-like bioactivity and structure. However, an analog of S2 with its penultimate residue substituted from S1 (S2(M); SGPYSFNSGLMFamide) exhibited loss of S2-type bioactivity and structural properties. Collectively, our data indicate that the C-terminal regions of S1 and S2 are the key determinants of their differing bioactivity. However, the N-terminal region of S2 may influence its bioactivity by conferring structural stability in solution. Thus, analysis of chimeric SALMFamides has revealed how neuropeptide bioactivity is determined by a complex interplay of sequence and conformation.
authors.affiliation: School of Biological & Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK; School of Science and Health, University of Western Sydney, Locked bag 1797, Penrith, 2751 Sydney, Australia.
authors.forename: Christopher E
authors.initials: CE
authors.lastname: Jones
authors.affiliation: School of Biological & Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
authors.forename: Claire B
authors.initials: CB
authors.lastname: Otara
authors.affiliation: School of Biological & Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
authors.forename: Nadine D
authors.initials: ND
authors.lastname: Younan
authors.affiliation: School of Biological & Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
authors.forename: John H
authors.initials: JH
authors.lastname: Viles
authors.affiliation: School of Biological & Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK. Electronic address: m.r.elphick@qmul.ac.uk.
authors.forename: Maurice R
authors.initials: MR
authors.lastname: Elphick
keywords: Circular dichroism spectroscopy; Echinoderm; Neuropeptide; Nuclear magnetic resonance spectroscopy; Pharmacology