title: Transcriptome analysis reveals the effect of oral contraceptive use on cervical cancer.
abstract: Differentially-expressed genes (DEGs) correlated to oral contraceptives (OCs) were identified by comparing the transcriptomes of cervical cancer patients who have taken OCs and those who have not. Their biological functions and relevance to clinical manifestations were investigated further in order to gain an understanding of the pathogenesis of cervical cancer and provide potential therapeutic targets. Level 3 RNA-sequencing (seq) data for cervical squamous-cell carcinoma and endocervical adenocarcinoma and the clinical information were downloaded from The Cancer Genome Atlas. The present study analyzed the RNA‑seq data and information on OC use of 35 patients [OC users (n=18) and those who have never used OCs (n=17)]. Student's t‑test was used in order to identify DEGs and the false discovery rate (FDR) was estimated by a Beta-Uniform Mixture model, which was adopted in multiple testing corrections. A functional enrichment analysis was performed with the Database for Annotation, Visualization and Integrated Discovery tool and BioCarta. A total of 80 DEGs were identified in OC users while FDR=0.3 was set as the cut-off value. The metabolic process and human telomerase RNA gene transcription were significantly upregulated in DEGs. Furthermore, secreted LY6/PLAUR domain containing 1 was identified to be correlated to the pathological response, while the synapse defective 1 Rho GTPase homolog 2 was found to be significantly associated with the histological grade and overall survival time. In conclusion, present study shed light on the effect of OC use on the oncogenesis of the cervix and may indicate novel approaches for a targeted therapy of cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital, Tongji University, Shanghai 200065, P.R. China.
authors.forename: Tian
authors.initials: T
authors.lastname: Gao
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital, Tongji University, Shanghai 200065, P.R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Women's Hospital School of Medicine, Zhejiang University, Hangzhou 310006, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital, Tongji University, Shanghai 200065, P.R. China.
authors.forename: Huaifang
authors.initials: H
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D016000:Cluster Analysis; D003276:Contraceptives, Oral / Q000494:pharmacology*; D030541:Databases, Genetic; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D017423:Sequence Analysis, RNA; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D003276:Contraceptives, Oral; D058851:GPI-Linked Proteins; C510169:LYPD1 protein, human; D025521:Tumor Suppressor Proteins; D020741:rho GTP-Binding Proteins
references.citation: Lancet. 2003 Apr 5;361(9364):1159-67
references.pmid: 12686037
references.citation: Br J Cancer. 2000 Apr;82(7):1332-8
references.pmid: 10755410
references.citation: Bioinformatics. 2003 Jul 1;19(10):1236-42
references.pmid: 12835267
references.citation: Breast Cancer Res Treat. 2012 Aug;135(1):79-91
references.pmid: 22535017
references.citation: Trends Cell Biol. 1998 Jul;8(7):282-8
references.pmid: 9714600
references.citation: Int J Gynecol Cancer. 2003 Mar-Apr;13(2):103-10
references.pmid: 12657108
references.citation: Am J Obstet Gynecol. 1999 Nov;181(5 Pt 1):1263-9
references.pmid: 10561657
references.citation: Am J Epidemiol. 2000 Aug 1;152(3):233-41
references.pmid: 10933270
references.citation: Biochimie. 2008 Jan;90(1):13-23
references.pmid: 17854971
references.citation: Rev Med Chir Soc Med Nat Iasi. 2012 Oct-Dec;116(4):1112-7
references.pmid: 23700898
references.citation: Lancet. 2007 Nov 10;370(9599):1609-21
references.pmid: 17993361
references.citation: Neoplasia. 2000 Nov-Dec;2(6):531-9
references.pmid: 11228546
references.citation: Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2490-5
references.pmid: 15699322
references.citation: Am J Obstet Gynecol. 1992 Oct;167(4 Pt 2):1177-84
references.pmid: 1415443
references.citation: Nat Protoc. 2009;4(1):44-57
references.pmid: 19131956
references.citation: Med Hypotheses. 2007;69(3):550-2
references.pmid: 17368751
references.citation: Anticancer Res. 2010 May;30(5):1559-64
references.pmid: 20592341
references.citation: Biochemistry (Mosc). 2006 Oct;71(10):1153-60
references.pmid: 17125464
references.citation: Carcinogenesis. 2002 Dec;23(12):2025-30
references.pmid: 12507925
references.citation: Life Sci. 2012 Nov 27;91(21-22):1122-5
references.pmid: 22369755
references.citation: N Engl J Med. 1990 Nov 15;323(20):1375-81
references.pmid: 2146499
references.citation: Auton Autacoid Pharmacol. 2008 Oct;28(4):109-16
references.pmid: 18764860
references.citation: Cancer Res. 2011 Apr 15;71(8):2988-99
references.pmid: 21363912