title: High-throughput monitoring of major cell functions by means of lensfree video microscopy.
abstract: Quantification of basic cell functions is a preliminary step to understand complex cellular mechanisms, for e.g., to test compatibility of biomaterials, to assess the effectiveness of drugs and siRNAs, and to control cell behavior. However, commonly used quantification methods are label-dependent, and end-point assays. As an alternative, using our lensfree video microscopy platform to perform high-throughput real-time monitoring of cell culture, we introduce specifically devised metrics that are capable of non-invasive quantification of cell functions such as cell-substrate adhesion, cell spreading, cell division, cell division orientation and cell death. Unlike existing methods, our platform and associated metrics embrace entire population of thousands of cells whilst monitoring the fate of every single cell within the population. This results in a high content description of cell functions that typically contains 25,000 - 900,000 measurements per experiment depending on cell density and period of observation. As proof of concept, we monitored cell-substrate adhesion and spreading kinetics of human Mesenchymal Stem Cells (hMSCs) and primary human fibroblasts, we determined the cell division orientation of hMSCs, and we observed the effect of transfection of siCellDeath (siRNA known to induce cell death) on hMSCs and human Osteo Sarcoma (U2OS) Cells.
authors.affiliation: 1] Univ. Grenoble Alpes, F-38000 Grenoble, France [2] CEA, LETI, MINATEC Campus, F-38054 Grenoble, France.
authors.forename: S Vinjimore
authors.initials: SV
authors.lastname: Kesavan
authors.affiliation: 1] Univ. Grenoble Alpes, F-38000 Grenoble, France [2] CEA, LETI, MINATEC Campus, F-38054 Grenoble, France.
authors.forename: F
authors.initials: F
authors.lastname: Momey
authors.affiliation: 1] Univ. Grenoble Alpes, F-38000 Grenoble, France [2] CEA, LETI, MINATEC Campus, F-38054 Grenoble, France.
authors.forename: O
authors.initials: O
authors.lastname: Cioni
authors.affiliation: Plateforme d'imagerie dynamique, Imagopole, Institut Pasteur, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: David-Watine
authors.affiliation: Plateforme d'imagerie dynamique, Imagopole, Institut Pasteur, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Dubrulle
authors.affiliation: Plateforme d'imagerie dynamique, Imagopole, Institut Pasteur, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Shorte
authors.affiliation: 1] Univ. Grenoble Alpes, F-38000 Grenoble, France [2] CEA, iRTSV-Biologie à Grande Echelle, F-38054 Grenoble, France [3] INSERM, U1038, F-38054 Grenoble, France.
authors.forename: E
authors.initials: E
authors.lastname: Sulpice
authors.affiliation: 1] Univ. Grenoble Alpes, F-38000 Grenoble, France [2] CEA, iRTSV-Biologie à Grande Echelle, F-38054 Grenoble, France [3] INSERM, U1038, F-38054 Grenoble, France.
authors.forename: D
authors.initials: D
authors.lastname: Freida
authors.affiliation: 1] University of Cergy-Pontoise, France [2] CMLA, ENS Cachan, France.
authors.forename: B
authors.initials: B
authors.lastname: Chalmond
authors.affiliation: 1] Univ. Grenoble Alpes, F-38000 Grenoble, France [2] CEA, LETI, MINATEC Campus, F-38054 Grenoble, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Dinten
authors.affiliation: 1] Univ. Grenoble Alpes, F-38000 Grenoble, France [2] CEA, iRTSV-Biologie à Grande Echelle, F-38054 Grenoble, France [3] INSERM, U1038, F-38054 Grenoble, France [4].
authors.forename: X
authors.initials: X
authors.lastname: Gidrol
authors.affiliation: 1] Univ. Grenoble Alpes, F-38000 Grenoble, France [2] CEA, LETI, MINATEC Campus, F-38054 Grenoble, France [3].
authors.forename: C
authors.initials: C
authors.lastname: Allier
mesh_terms: D002448:Cell Adhesion; D002452:Cell Count; D016923:Cell Death / Q000235:genetics; D002455:Cell Division; D045744:Cell Line, Tumor; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods*; D010006:Osteoblasts / Q000378:metabolism* / Q000473:pathology; D061251:Primary Cell Culture; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014741:Video Recording / Q000295:instrumentation / Q000379:methods*
chemical_list: D034741:RNA, Small Interfering
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