title: Introduction of correlative light and airSEM™ microscopy imaging for tissue research under ambient conditions.
abstract: A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellular components while minimizing artifacts. We introduce the application of a novel scanning electron microscope (airSEM™) in conjunction with light microscopy for functional analysis of tissue preparations at nanometric resolution (<10 nm) and under ambient conditions. Our metal-staining protocols enable easy and detailed visualization of tissues and their extracellular scaffolds. A multimodality imaging setup, featuring airSEM™ and a light microscope on the same platform, provides a convenient and easy-to-use system for obtaining structural and functional correlative data. The airSEM™ imaging station complements other existing imaging solutions and shows great potential for studies of complex biological systems.
authors.affiliation: 1] Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel [2].
authors.forename: Inna
authors.initials: I
authors.lastname: Solomonov
authors.affiliation: 1] Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel [2].
authors.forename: Dalit
authors.initials: D
authors.lastname: Talmi-Frank
authors.affiliation: B-nano LTD, Rehovot 76326, Israel.
authors.forename: Yonat
authors.initials: Y
authors.lastname: Milstein
authors.affiliation: B-nano LTD, Rehovot 76326, Israel.
authors.forename: Sefi
authors.initials: S
authors.lastname: Addadi
authors.affiliation: Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Anna
authors.initials: A
authors.lastname: Aloshin
authors.affiliation: Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Irit
authors.initials: I
authors.lastname: Sagi
mesh_terms: D000818:Animals; D002232:Carbocyanines; D024042:Collagen Type I / Q000302:isolation & purification / Q000648:ultrastructure; D004396:Coloring Agents; D055032:Electron Microscope Tomography / Q000295:instrumentation* / Q000379:methods; D005109:Extracellular Matrix / Q000648:ultrastructure; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D008168:Lung / Q000648:ultrastructure*; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation* / Q000379:methods; D009942:Organometallic Compounds; D051381:Rats; D012430:Ruthenium Red; D013194:Staining and Labeling / Q000379:methods; D013623:Tail / Q000737:chemistry
chemical_list: D002232:Carbocyanines; D024042:Collagen Type I; D004396:Coloring Agents; D009942:Organometallic Compounds; C089187:cyanine dye 3; D012430:Ruthenium Red; C005460:uranyl acetate
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