title: Synthesis of novel gold mesoflowers as SERS tags for immunoassay with improved sensitivity.
abstract: A new class of flowerlike gold mesostructure in high yield is successfully synthesized through a facile one-step route using ascorbic acid as a reducing agent of gold salt with cetyltrimethylammonium chloride (CTAC) as surfactant. The as-prepared Au particles have spherical profiles with an averaged diameter of 770 ± 50 nm, but showing a highly rough surface consisting of many irregular and randomly arranged protrusions. The Au mesoflowers exhibit strong surface-enhanced effects and near-infrared absorption which were utilized in the design of efficient surface-enhanced Raman scattering (SERS) tags as immunosensors for immunoassay with improved sensitivity. The experimental results indicate that a good linear relationship is found between the peak intensity at 1071 cm(-1) and the logarithm of H-IgG concentration in the range between 1 ng/mL and 1 fg/mL, and the limit of detection (LOD) is 1 fg/mL.
authors.affiliation: Key Lab Organic Electronics & Information Displays (KLOEID), and Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications , Nanjing 210023, China.
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Song
authors.forename: Linghua
authors.initials: L
authors.lastname: Min
authors.forename: Ni
authors.initials: N
authors.lastname: Zhou
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Yang
authors.forename: Shao
authors.initials: S
authors.lastname: Su
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
mesh_terms: D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D013056:Spectrophotometry, Ultraviolet; D019265:Spectroscopy, Near-Infrared; D013059:Spectrum Analysis, Raman; D014961:X-Ray Diffraction
chemical_list: D006046:Gold
keywords: SERS tags; flowerlike gold mesostructures; immunoassay; surface-enhanced Raman scattering