title: trans-Platinum(II) complex of 3-aminoflavone - synthesis, X-ray crystal structure and biological activities in vitro.
abstract: This paper describes the synthesis of trans-bis-(3-aminoflavone)dichloridoplatinum(ii) (trans-Pt(3-af)2Cl2; TCAP) for use as a potential anticancer compound, and the evaluation of its structure by elemental and spectral analyses, and X-ray crystallography. The complex demonstrated a significant cytotoxic effect against human and murine cancer cell lines, as well as weaker toxicity towards healthy cells (human peripheral blood lymphocytes) in comparison with cisplatin. Various biochemical and morphological methods confirm that the proapoptotic activity of trans-Pt(3-af)2Cl2 is markedly higher than the reference cisplatin. Our results suggest that trans-Pt(3-af)2Cl2 may have a different antitumour specificity from that of cisplatin.
authors.affiliation: Department of Bioinorganic Chemistry, 1 Muszyńskiego St., Medical University, 90-151 Łódź, Poland. justyn.ochocki@umed.lodz.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Fabijańska
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Studzian
authors.forename: Leszek
authors.initials: L
authors.lastname: Szmigiero
authors.forename: Agnieszka J
authors.initials: AJ
authors.lastname: Rybarczyk-Pirek
authors.forename: Arno
authors.initials: A
authors.lastname: Pfitzner
authors.forename: Barbara
authors.initials: B
authors.lastname: Cebula-Obrzut
authors.forename: Piotr
authors.initials: P
authors.lastname: Smolewski
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Zyner
authors.forename: Justyn
authors.initials: J
authors.lastname: Ochocki
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D018360:Crystallography, X-Ray; D053938:DNA Fragmentation / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry*; D006801:Humans; D007536:Isomerism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D010984:Platinum / Q000737:chemistry*
chemical_list: C488624:3-aminoflavone; D056831:Coordination Complexes; D005419:Flavonoids; D010984:Platinum; D053148:Caspase 3