title: The impact of enterocin AS-48 on the shelf-life and safety of sardines (Sardina pilchardus) under different storage conditions.
abstract: The purpose of this study was to determine the effect of enterocin AS-48, packaged under normal atmosphere (NA), vacuum (VP) or modified atmosphere (MAP) on the shelf life and safety of fresh sardines (Sardina pilchardus) stored at 5 °C. We studied the effect of these hurdles, alone or combined, on the relevant autochthonous bacterial populations. Total volatile basic nitrogen (TVB-N) content was used as indicative of freshness. Levels of biogenic amines cadaverine, putrescine, tyramine, and histamine were also determined. The application of AS-48 did not reduce the mesophilic, psychrotrophic, or Gram negative bacteria viable cell counts under any of the storage conditions tested. AS-48 did cause significant reductions in viable staphylococci counts, especially under VP. In sardines under NA treated with AS-48, the populations of histamine- and tyramine-forming total and lactic acid bacteria (LAB) showed no significant reductions. MAP or VP with AS-48 allowed reductions (significant at some storage times) in histamine- and tyramine-forming LAB. The TVB-N content was also reduced under normal atmosphere and, especially, in sardines stored under MAP. The most interesting results are those concerning the decrease (by several fold) in the levels of the biogenic amines cadaverine, putrescine, tyramine, and histamine determined after treatment with AS-48.
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Fuente Nueva s/n, 19071 Granada, Spain; Instituto de Biotecnología, Universidad de Granada, 18071 Granada, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Ananou
authors.affiliation: Instituto de Biotecnología, Universidad de Granada, 18071 Granada, Spain.
authors.forename: H
authors.initials: H
authors.lastname: Zentar
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Fuente Nueva s/n, 19071 Granada, Spain; Instituto de Biotecnología, Universidad de Granada, 18071 Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Bueno
authors.affiliation: Área de Microbiología, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje Las Lagunillas, Jaén, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Gálvez
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Fuente Nueva s/n, 19071 Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Maqueda
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Fuente Nueva s/n, 19071 Granada, Spain; Instituto de Biotecnología, Universidad de Granada, 18071 Granada, Spain. Electronic address: evavm@ugr.es.
authors.forename: E
authors.initials: E
authors.lastname: Valdivia
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D001430:Bacteriocins / Q000032:analysis / Q000494:pharmacology*; D005396:Fish Products / Q000032:analysis* / Q000382:microbiology; D005399:Fishes; D005503:Food Additives / Q000032:analysis* / Q000494:pharmacology; D005519:Food Preservation / Q000379:methods*; D059022:Food Safety; D061353:Food Storage
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D005503:Food Additives; C107799:enterocin A
keywords: Biogenic amines; Enterocin AS-48; Fish quality; Sardina pilchardus; Shelf-life