title: Astaxanthin induction in Microalga H. pluvialis with flat panel airlift photobioreactors under indoor and outdoor conditions.
abstract: Astaxanthin was induced from Haematococcus pluvialis (NIES-144) under indoor and outdoor conditions using 17-, 50-, and 90-L flat-panel airlift photobioreactors (FP-APBRs). Preliminary experiments in 1.5-L bubble column photobioreactors (BC-PBRs) revealed that sterilized clean water with 3% CO2 aeration (1.47 cm(3) s(-1) CO2 loading) could best encourage astaxanthin accumulation at 18.21 g m(-3) (3.63% by weight). Operating 17-L FP-APBRs with these bubble column parameters under indoor conditions could further enhance astaxanthin to 26.63 g m(-3) (5.34% by weight). This was potentially due to the inherited up-lift force from the reactor that helped avoid cell precipitation by allowing the cells to be circulated within the reactor. In addition, the various sizes of FP-APBRs exhibited similar performance, implying a potential scale-up opportunity. However, similar operation under outdoor condition exhibited slightly poorer performance due to the light inhibition effect. The best outdoor performance was obtained with the FP-APBR covered with one layer of shading net, where 20.11 g m(-3) (4.47% by weight) of astaxanthin was resulted.
authors.affiliation: a Department of Chemical Engineering, Faculty of Engineering , Chulalongkorn University , Bangkok , Thailand.
authors.forename: Woradej
authors.initials: W
authors.lastname: Poonkum
authors.forename: Sorawit
authors.initials: S
authors.lastname: Powtongsook
authors.forename: Prasert
authors.initials: P
authors.lastname: Pavasant
mesh_terms: D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002245:Carbon Dioxide / Q000378:metabolism; D002452:Cell Count; D000460:Chlorophyta / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D004867:Equipment Design; D058086:Microalgae / Q000378:metabolism*; D058433:Photobioreactors; D024341:Xanthophylls / Q000378:metabolism
chemical_list: D003470:Culture Media; D024341:Xanthophylls; D002245:Carbon Dioxide; C005948:astaxanthine
keywords: Haematococcus pluvialis; carotenoid; mature cyst; microalgae; pigment; scale-up