title: Cellular and proteomics analysis of the endomembrane system from the unicellular Entamoeba histolytica.
abstract: Entamoeba histolytica is the protozoan parasite agent of amoebiasis, an infectious disease of the human intestine and liver. Specific active pathogenic factors are secreted toward the external milieu upon interaction of the parasite with human tissue. Trafficking dynamics and secretion of these factors is not known and characterization of the dynamics interplay of subcellular compartments such as the ER or Golgi apparatus is still pending. In this work, we took advantage of cell fractionation and a wide proteomic analysis to search for principal components of the endomembrane system in E. histolytica. Over 1500 proteins were identified and the two top categories contained components of trafficking machinery and GTPases. Trafficking related proteins account for over 100 markers from the ER, Golgi, MVB, and retromers. The lack of important components supporting Golgi polarization was also highlighted. The data further describe principal components of the endosomal traffic highlighting Alix in isolated vesicles and during parasite division.
  
  
  CONCLUSIONS
  This work represents the first in-depth proteomics analysis of subcellular compartments in E. histolytica and allows a detailed map of vesicle traffic components in an ancient single-cell organism that lacks a stereotypical ER and Golgi apparatus to be established.
authors.affiliation: Institut Pasteur, Cell Biology of Parasitism Unit, F-75015 Paris, France; INSERM U786, F-75015 Paris, France; Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, F-75015 Paris, France. Electronic address: doranda.perdomobastidas@mail.mcgill.ca.
authors.forename: Doranda
authors.initials: D
authors.lastname: Perdomo
authors.affiliation: Institut Pasteur, Cell Biology of Parasitism Unit, F-75015 Paris, France; INSERM U786, F-75015 Paris, France.
authors.forename: Nawel
authors.initials: N
authors.lastname: Aït-Ammar
authors.affiliation: Institut Pasteur, Cell Biology of Parasitism Unit, F-75015 Paris, France; INSERM U786, F-75015 Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Syan
authors.affiliation: Institut Pasteur, Imagopole, Plate-Forme de Microscopie Ultrastructurale, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Sachse
authors.affiliation: National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Gagan Deep
authors.initials: GD
authors.lastname: Jhingan
authors.affiliation: Institut Pasteur, Cell Biology of Parasitism Unit, F-75015 Paris, France; INSERM U786, F-75015 Paris, France. Electronic address: nguillen@pasteur.fr.
authors.forename: Nancy
authors.initials: N
authors.lastname: Guillén
mesh_terms: D004721:Endoplasmic Reticulum / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D021381:Protein Transport; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015800:Protozoan Proteins / Q000378:metabolism*
chemical_list: D020543:Proteome; D015800:Protozoan Proteins
keywords: Endomembrane system; Entamoeba; Traffic