title: [Influence of huatanjiangqi prescription on multidrug resistance-associated protein 1 in human bronchial epithelial cells].
abstract: OBJECTIVE
  To investigate the effect of Huatanjiangqi prescription (Sinapis Semen, Perillae Fructus, Cynanchi Stauntonii Rhizoma et Radix, Inulae Herba, Angelicae Sinensis Radix, Honey-fried Ephedrae Herba) on multidrug resistance-associated protein 1 (MRP1) in human bronchial epithelial cells.
  
  
  METHODS
  The human bronchial epithelial cells line 16HBE140- was used to analyze the in vitro effect of Huatanjiangqi prescription on MRP1 transport. 5-CFDA was used as a model MRP1 substrate and was measured with flow cytometry. The mRNA expression of MRP1 was detected by real-time PCR.
  
  
  RESULTS
  Huatanjiangqi prescription could promote the proliferation of human bronchial epithelial cells 16HBE140- in a certain range of concentration; Compared with the control group (5-CFDA), low, medium and high concentration (100, 1 000, 2 000 microg/mL) of Huatanjiangqi prescription on MRP1 function were increased by 22.59%, 47.14% and 68.36%, respectively; Huatanjiangqi prescription could concentration-dependently induce the expression of MRP1 mRNA, medium and high concentration could induce a significant difference.
  
  
  CONCLUSIONS
  Huatanjiangqi prescription can improve MRP1 efflux function and mRNA levels in a concetration-dependent manner.
authors.forename: Chen-Yin
authors.initials: CY
authors.lastname: Wang
authors.forename: Dian-Lei
authors.initials: DL
authors.lastname: Wang
authors.forename: Xiu-Hua
authors.initials: XH
authors.lastname: Tao
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Yang
authors.forename: Jin-Pei
authors.initials: JP
authors.lastname: Chen
authors.forename: Yin
authors.initials: Y
authors.lastname: Cao
mesh_terms: D001980:Bronchi / Q000166:cytology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D021381:Protein Transport / Q000187:drug effects; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005452:Fluoresceins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger; C421109:multidrug resistance-associated protein 1