title: [Stimulating Toll-like receptor 2 promotes the cell apoptosis through augmenting the expression of NIPK in lung epithelial cells].
abstract: OBJECTIVE
  To investigate if stimulating Toll-like receptor 2 (TLR2) specifically induces the cell apoptosis in mouse lung epithelial cells (MLE-12) and related potential mechanism.
  
  
  METHODS
  TLR2 was stimulated by 0, 1, 3.3, 10, 33 ng/mL PAM3CYS4 for 24 hours, or 33 ng/mL PAM3CYS4 for 0, 8, 16, 24, 36, 48 hours, respectively. The expressions of neuronal cell death inducible putative kinase (NIPK) and cleaved caspase-3 were detected by Western blotting; the number of apoptotic cells was counted by TUNEL staining in MLE-12 lung epithelial cells.
  
  
  RESULTS
  Responding to TLR2-specific stimulation, the expressions of NIPK and cleaved caspase-3 were significantly elevated, and the number of apoptotic cells increased. Inhibiting NIPK by siRNA transfection decreased the expression of cleaved caspase-3 and the number of apoptotic cells.
  
  
  CONCLUSIONS
  Stimulating TLR2 signaling using TLR2-specific ligands PAM3CYS4 promotes the cell apoptosis through increasing the expression of NIPK.
authors.affiliation: Department of Oncology, Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100050, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Centre, Beijing 100094, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Centre, Beijing 100094, China.
authors.forename: Yongzhi
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Centre, Beijing 100094, China.
authors.forename: Jiaping
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D020287:In Situ Nick-End Labeling; D055666:Lipopeptides / Q000494:pharmacology; D008168:Lung / Q000166:cytology; D051379:Mice; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D013997:Time Factors; D051195:Toll-Like Receptor 2 / Q000819:agonists / Q000378:metabolism*
chemical_list: D055666:Lipopeptides; D051195:Toll-Like Receptor 2; D011494:Protein Kinases; C119271:neuronal cell death inducible putative kinase; D053148:Caspase 3