title: Forchlorfenuron detection based on its inhibitory effect towards catalase immobilized on boron nitride substrate.
abstract: An enzymatic procedure based on a catalase biosensor for the detection of forchlorfenuron (CPPU) has been reported in this work. Catalase was immobilized on boron nitride (BN) sheets dispersed in chitosan by adsorption. The immobilized catalase exhibited direct electron transfer character and excellent electrocatalytic activity towards H2O2 reduction. After introducing CPPU into the H2O2 containing phosphate buffer solution, the catalase-catalyzed H2O2 reduction current decreased. By measuring the current decrease, CPPU can be determined in the range of 0.5-10.0 µM with the detection limit of 0.07 μM. The non-competitive inhibition behavior of CPPU towards catalase was verified by the Lineweaver-Burk plots. Long stability character has been ascribed to this biosensor. Possible use of this biosensor in flow systems is illustrated. The proposed biosensor has been successfully applied to CPPU determination in fruits samples with satisfactory results.
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Cai
authors.affiliation: Institute of Optoelectronics & Nanomaterials, College of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Huijie
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
authors.forename: Jiaqian
authors.initials: J
authors.lastname: Tang
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China. Electronic address: xyhu@yzu.edu.cn.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Optoelectronics & Nanomaterials, College of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: zeng.haibo.nano@gmail.com.
authors.forename: Haibo
authors.initials: H
authors.lastname: Zeng
mesh_terms: D015374:Biosensing Techniques; D001896:Boron Compounds / Q000737:chemistry; D002374:Catalase / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D010671:Phenylurea Compounds / Q000737:chemistry / Q000302:isolation & purification*; D011725:Pyridines / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D001896:Boron Compounds; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D010671:Phenylurea Compounds; D011725:Pyridines; C017282:boron nitride; D002374:Catalase; C116093:N-(2-chloro-4-pyridyl)-N'-phenylurea
keywords: Boron nitride; Catalase; Forchlorfenuron; Inhibition