title: Starved anammox cells are less resistant to NO₂⁻ inhibition.
abstract: Anaerobic ammonium oxidizing (anammox) bacteria are be inhibited by their terminal electron acceptor, nitrite. Serious nitrite inhibition of the anammox bacteria occurs if the exposure coincides with the absence of the electron donating substrate, ammonium and pH < 7.2. Starvation of biomass occurs during underloading of bioreactors or biomass storage. This work investigated the effect of starvation on the sensitivity of anammox bacteria to nitrite exposure. Batch activity tests were carried out evaluating the response of anammox biomass subjected to different levels of starvation upon exposure to nitrite in the presence and absence of ammonium (active- and resting-cells, respectively). The response of the bacteria was evaluated by measuring the specific anammox activity and the evolution of the ATP content in the biomass over time. The 50% inhibitory concentrations of nitrite in starved- and fresh-resting-cells was 7 mg N L(-1) and 52 mg N L(-1), respectively. By contrast, only moderate nitrite inhibition occurred to starved anammox biomass when exposed to nitrite and ammonium simultaneously. Maximum ATP levels were observed in fresh cells. The ATP content in starved resting cells peaked 2-3 h after addition of NO2(-)(-). The response was hindered in cells starved for long periods. These findings agreed with a bioreactor study in which underloading of anammox biomass (0.10 g N L(-1) d(-1)) decreased its tolerance to a nitrite (only) exposure (101 mg NO2(-)-N L(-1)) and completely disrupted the N removal capacity of the biomass. A similar accumulation of 108 mg NO2(-)-N L(-1) after operation at 0.95 g N L(-1) d(-1) did not cause observable inhibition of the bacteria. The results taken as a whole demonstrate that starved anammox biomass is highly sensitive to nitrite toxicity. An explanation is proposed based on energy requirements to translocate nitrite in the cell.
authors.affiliation: Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 210011, Tucson, AZ, USA. Electronic address: jcarvaja@email.arizona.edu.
authors.forename: José M
authors.initials: JM
authors.lastname: Carvajal-Arroyo
authors.affiliation: Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 210011, Tucson, AZ, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Puyol
authors.affiliation: Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 210011, Tucson, AZ, USA.
authors.forename: Guangbin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 210011, Tucson, AZ, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Swartwout
authors.affiliation: Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 210011, Tucson, AZ, USA.
authors.forename: Reyes
authors.initials: R
authors.lastname: Sierra-Álvarez
authors.affiliation: Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 210011, Tucson, AZ, USA.
authors.forename: Jim A
authors.initials: JA
authors.lastname: Field
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D064751:Ammonium Compounds / Q000378:metabolism*; D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000166:cytology / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D009573:Nitrites / Q000378:metabolism*; D016877:Oxidants; D012722:Sewage / Q000382:microbiology
chemical_list: D064751:Ammonium Compounds; D009573:Nitrites; D016877:Oxidants; D012722:Sewage; D000255:Adenosine Triphosphate
keywords: ATP; Anaerobic ammonium oxidation; Autotrophic nitrogen removal; Granular sludge; Inhibition; Underloading