title: Secretomes are a potential source of molecular targets for cancer therapies and indicate that APOE is a candidate biomarker for lung adenocarcinoma metastasis.
abstract: Identifying patients at high risk of metastasis is a major challenge in lung adenocarcinoma (ADC) therapy, therefore discovery of noninvasive biomarkers and therapeutic targets is urgent. We found significant differences between the secretomes of differentially expressed proteins in lung ADC cell lines, clinical tissue samples and serum plasma samples with high and low metastatic potential. In particular, Apolipoprotein E (APOE) levels were three-times greater in cells with lymph node metastases (LNM) than those without. Our study indicates that APOE is a potential indicator of metastatic lung ADC and that secretomes may offer a valuable resource for biomarkers of lung ADC with LNM.
authors.affiliation: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, 110001, China.
authors.forename: Zan
authors.initials: Z
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Fengxia
authors.initials: F
authors.lastname: Hao
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Lou
authors.forename: Xiuwei
authors.initials: X
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Ting
authors.initials: T
authors.lastname: Xiao
authors.forename: Longhai
authors.initials: L
authors.lastname: Yang
authors.forename: Qingchang
authors.initials: Q
authors.lastname: Li
authors.forename: Xueshan
authors.initials: X
authors.lastname: Qiu
authors.forename: Enhua
authors.initials: E
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D000704:Analysis of Variance; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism*; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000175:diagnosis* / Q000235:genetics; D013058:Mass Spectrometry; D046888:Tissue Array Analysis
chemical_list: D001057:Apolipoproteins E; D014408:Biomarkers, Tumor
references.citation: J Clin Oncol. 2007 Feb 10;25(5):472-8
references.pmid: 17290054
references.citation: Proteomics. 2005 Jul;5(10):2587-601
references.pmid: 15912508
references.citation: Br J Cancer. 2009 Jun 16;100(12):1966-74
references.pmid: 19455140
references.citation: Annu Rev Genomics Hum Genet. 2000;1:507-37
references.pmid: 11701639
references.citation: Proteomics. 2012 Feb;12(4-5):722-35
references.pmid: 22247067
references.citation: PLoS One. 2011 Apr 12;6(4):e18567
references.pmid: 21533267
references.citation: Nature. 2008 Apr 3;452(7187):571-9
references.pmid: 18385731
references.citation: PLoS Comput Biol. 2010 Jan 15;6(1):e1000641
references.pmid: 20090828
references.citation: J Proteome Res. 2008 Apr;7(4):1379-87
references.pmid: 18290608
references.citation: Proteome Sci. 2010 Jun 14;8:31
references.pmid: 20546617
references.citation: Mol Cell Proteomics. 2010 Jun;9(6):1100-17
references.pmid: 20124221
references.citation: Lung Cancer. 2011 Jan;71(1):28-33
references.pmid: 20430468
references.citation: Mol Cell Proteomics. 2007 Sep;6(9):1638-55
references.pmid: 17533153
references.citation: J Proteome Res. 2012 Mar 2;11(3):1759-72
references.pmid: 22204653
references.citation: Oncol Rep. 2008 Dec;20(6):1313-9
references.pmid: 19020708
references.citation: Cancer Res Treat. 2012 Jun;44(2):121-6
references.pmid: 22802750
references.citation: J Proteome Res. 2007 Mar;6(3):1083-92
references.pmid: 17284061
references.citation: Oncol Lett. 2013 Jul;6(1):43-48
references.pmid: 23946775
references.citation: Cancer Res. 2004 Apr 1;64(7):2397-405
references.pmid: 15059891
references.citation: Haematologica. 2013 Feb;98(2):296-304
references.pmid: 22875629
references.citation: J Biol Chem. 1992 Jun 5;267(16):10931-4
references.pmid: 1375931
references.citation: Microbiol Mol Biol Rev. 2000 Sep;64(3):515-47
references.pmid: 10974125
references.citation: Aging Cell. 2011 Jun;10(3):533-41
references.pmid: 21332925
references.citation: BMC Genomics. 2011 Nov 30;12 Suppl 3:S24
references.pmid: 22369099
references.citation: Mol Cell Proteomics. 2009 Dec;8(12):2746-58
references.pmid: 19776420
references.citation: BMC Bioinformatics. 2010 Jan 26;11:53
references.pmid: 20102611
references.citation: J Proteome Res. 2010 Jan;9(1):545-55
references.pmid: 19924834
references.citation: N Engl J Med. 1971 Nov 18;285(21):1182-6
references.pmid: 4938153
references.citation: Clin Chim Acta. 2013 Jun 5;421:230-5
references.pmid: 23523589
references.citation: J Transl Med. 2008 Sep 17;6:52
references.pmid: 18796163
references.citation: BMC Med Genomics. 2011 Jan 06;4:2
references.pmid: 21211025
references.citation: J Proteomics. 2010 Nov 10;73(12):2291-305
references.pmid: 20637910
references.citation: Cancer Res. 2009 Oct 15;69(20):8001-8
references.pmid: 19808960
references.citation: PLoS Comput Biol. 2010 Jan 15;6(1):e1000639
references.pmid: 20090827
references.citation: PLoS One. 2012;7(10):e47469
references.pmid: 23094052
references.citation: Nature. 2000 Sep 14;407(6801):249-57
references.pmid: 11001068
references.citation: PLoS One. 2013 May 01;8(5):e54659
references.pmid: 23658677
references.citation: CA Cancer J Clin. 2013 Mar-Apr;63(2):107-17
references.pmid: 23315954
references.citation: Proteome Sci. 2013 May 20;11(1):22
references.pmid: 23688336