title: Selective uptake of cylindrical poly(2-oxazoline) brush-antiDEC205 antibody-OVA antigen conjugates into DEC-positive dendritic cells and subsequent T-cell activation.
abstract: To achieve specific cell targeting by various receptors for oligosaccharides or antibodies, a carrier must not be taken up by any of the very many different cells and needs functional groups prone to clean conjugation chemistry to derive well-defined structures with a high biological specificity. A polymeric nanocarrier is presented that consists of a cylindrical brush polymer with poly-2-oxazoline side chains carrying an azide functional group on each of the many side chain ends. After click conjugation of dye and an anti-DEC205 antibody to the periphery of the cylindrical brush polymer, antibody-mediated specific binding and uptake into DEC205(+) -positive mouse bone marrow-derived dendritic cells (BMDC) was observed, whereas binding and uptake by DEC205(-) negative BMDC and non-DC was essentially absent. Additional conjugation of an antigen peptide yielded a multifunctional polymer structure with a much stronger antigen-specific T-cell stimulatory capacity of pretreated BMDC than application of antigen or polymer-antigen conjugate.
authors.affiliation: Institute for Physical Chemistry, University of Mainz, Jakob-Welder Weg 11, 55099 Mainz (Germany); Graduate School Materials Science, Staudinger Weg 9, 55128 Mainz (Germany).
authors.forename: Jasmin
authors.initials: J
authors.lastname: Bühler
authors.forename: Sabine
authors.initials: S
authors.lastname: Gietzen
authors.forename: Anika
authors.initials: A
authors.lastname: Reuter
authors.forename: Cinja
authors.initials: C
authors.lastname: Kappel
authors.forename: Karl
authors.initials: K
authors.lastname: Fischer
authors.forename: Sandra
authors.initials: S
authors.lastname: Decker
authors.forename: David
authors.initials: D
authors.lastname: Schäffel
authors.forename: Kaloian
authors.initials: K
authors.lastname: Koynov
authors.forename: Matthias
authors.initials: M
authors.lastname: Bros
authors.forename: Ingrid
authors.initials: I
authors.lastname: Tubbe
authors.forename: Stephan
authors.initials: S
authors.lastname: Grabbe
authors.forename: Manfred
authors.initials: M
authors.lastname: Schmidt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D018796:Immunoconjugates / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology*; D037181:Lectins, C-Type / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D010080:Oxazoles / Q000737:chemistry / Q000276:immunology; D011108:Polymers / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D015703:Antigens, CD; C093569:DEC-205 receptor; D018796:Immunoconjugates; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D010080:Oxazoles; D011108:Polymers; D011956:Receptors, Cell Surface; C577913:poly(2-oxazoline); D010047:Ovalbumin
keywords: T-cells; brush polymers; cancer therapy; dendritic cells; nanocarriers