title: Buyang Huanwu Decoction () reduces infarct volume and enhances estradiol and estradiol receptor concentration in ovariectomized rats after middle cerebral artery occlusion.
abstract: OBJECTIVE
  To investigate the effect of Buyang Huanwu Decoction (, BYHWD) on estradiol (E2) and estradiol receptor (ER) in serum and brain in ovariectomized rats after middle cerebral artery occlusion (MCAO).
  
  
  METHODS
  Adult female rats were ovariectomized and focal cerebral ischemic was induced by MCAO. Rats were randomly divided into normal, ovariectomy (OVX), MCAO, OVX+MCAO, OVX+MCAO+E2, and OVX+MCAO+BYHWD group. Rats were administered BYHWD 5 g/kg daily, estradiol valerate 500 μg/kg per day or distilled water for 7 consecutive days. Neuronal function and infarct volume were measured on day 7 after artery occlusion, and E2 and ER concentration in serum and brain were checked by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  BYHWD significantly improved the neurological behavior, reduced the infarction volume, increased E2 concentration in serum and brain, and increased ER concentration in the brain in ovariectomized rats after MCAO.
  
  
  CONCLUSIONS
  The neuroprotective effects of BYHWD are associated with estrogen and its receptor.
authors.affiliation: Key Laboratory of Internal Medicine, Hunan University of Traditional Chinese Medicine, Changsha, 410007, China.
authors.forename: Bai-yan
authors.initials: BY
authors.lastname: Liu
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Song
authors.forename: Jian
authors.initials: J
authors.lastname: Yi
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Yue
authors.initials: Y
authors.lastname: Yu
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Guang-xian
authors.initials: GX
authors.lastname: Cai
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000150:complications / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D002544:Cerebral Infarction / Q000150:complications / Q000188:drug therapy* / Q000473:pathology* / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004958:Estradiol / Q000097:blood*; D005260:Female; D020244:Infarction, Middle Cerebral Artery / Q000150:complications* / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D010052:Ovariectomy; D017208:Rats, Wistar; D011959:Receptors, Estradiol / Q000097:blood*
chemical_list: D004365:Drugs, Chinese Herbal; D011959:Receptors, Estradiol; C460102:buyang huanwu; D004958:Estradiol
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