title: Anti-inflammatory and antiplatelet activities of plasma are conserved across twelve mammalian species.
abstract: Human plasma inhibits arachidonic acid metabolism and platelet aggregation. This helps human form a haemostatic control system that prevents the progress of certain aggregatory or inflammatory reactions. Whether this property of plasma is unique to human or extends to other species is not well known. It is speculated that this protective ability of plasma remains evolutionarily conserved in different mammals. In order to confirm this, the effect of plasma from 12 different mammalian species was investigated for its inhibitory potential against arachidonic acid metabolism and platelet aggregation. Metabolism of arachidonic acid by cyclooxygenase and lipoxygenase pathways was studies using radio-immuno assay and thin layer chromatography while platelet aggregation in the plasma of various mammals was monitored following turbedmetric method in a dual channel aggregometer. Results indicate that inhibition of AA metabolism and platelet aggregation is a common feature of plasma obtained from different mammalian species, although there exists large interspecies variation. This shows that besides human, other mammals also possess general protective mechanisms against various aggregatory and inflammatory conditions and this anti-inflammatory property of the plasma is evolutionarily conserved in mammalian species. The most likely candidates responsible for these properties of plasma include haptoglobin, albumin and lipoproteins.
authors.affiliation: PAP RSB Institute of Health Sciences, University Brunei Darussalam, Gadong BE1410, Bander Seri Begawan, Brunei Darussalam. sagheer.ahmed@ubd.edu.bn.
authors.forename: Sagheer
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: PAP RSB Institute of Health Sciences, University Brunei Darussalam, Gadong BE1410, Bander Seri Begawan, Brunei Darussalam. saimaa.gul@gmail.com.
authors.forename: Saima
authors.initials: S
authors.lastname: Gul
authors.affiliation: PAP RSB Institute of Health Sciences, University Brunei Darussalam, Gadong BE1410, Bander Seri Begawan, Brunei Darussalam. fazean.idris@ubd.edu.bn.
authors.forename: Fazean
authors.initials: F
authors.lastname: Idris
authors.affiliation: Department of Biotechnology & Informtics, Baluchistan University of Information Technology, Engineering & Management Sciences, Quetta 87300, Pakistan. abrarbangash176@gmail.com.
authors.forename: Abrar
authors.initials: A
authors.lastname: Hussain
authors.affiliation: The Patent Office, Karachi 74400, Pakistan. ahirzia@gmail.com.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Zia-Ul-Haq
authors.affiliation: Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, Selangor 43400, Malaysia. hawazej@agri.upm.edu.my.
authors.forename: Hawa Z E
authors.initials: HZ
authors.lastname: Jaafar
authors.affiliation: Department of Medical and Surgical Specialities, Transilvania University of Brasov, Brasov 500036, Romania. moga.og@gmail.com.
authors.forename: Marius
authors.initials: M
authors.lastname: Moga
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism; D005075:Biological Evolution; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D006487:Hemostasis; D006801:Humans; D020128:Inhibitory Concentration 50; D008084:Lipoxygenase / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D010949:Plasma / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D010975:Platelet Aggregation Inhibitors; D016718:Arachidonic Acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases
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