title: Proteomics informed by transcriptomics identifies novel secreted proteins in Dermacentor andersoni saliva.
abstract: Dermacentor andersoni, known as the Rocky Mountain wood tick, is found in the western United States and transmits pathogens that cause diseases of veterinary and public health importance including Rocky Mountain spotted fever, tularemia, Colorado tick fever and bovine anaplasmosis. Tick saliva is known to modulate both innate and acquired immune responses, enabling ticks to feed for several days without detection. During feeding ticks subvert host defences such as hemostasis and inflammation, which would otherwise result in coagulation, wound repair and rejection of the tick. Molecular characterization of the proteins and pharmacological molecules secreted in tick saliva offers an opportunity to develop tick vaccines as an alternative to the use of acaricides, as well as new anti-inflammatory drugs. We performed proteomics informed by transcriptomics to identify D. andersoni saliva proteins that are secreted during feeding. The transcript data generated a database of 21,797 consensus sequences, which we used to identify 677 proteins secreted in the saliva of D. andersoni ticks fed for 2 and 5days, following proteomic investigations of whole saliva using mass spectrometry. Salivary gland transcript levels of unfed ticks were compared with 2 and 5day fed ticks to identify genes upregulated early during tick feeding. We cross-referenced the proteomic data with the transcriptomic data to identify 157 proteins of interest for immunomodulation and blood feeding. Proteins of unknown function as well as known immunomodulators were identified.
authors.affiliation: Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, USA; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA.
authors.forename: Lwiindi
authors.initials: L
authors.lastname: Mudenda
authors.affiliation: Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, USA; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA.
authors.forename: Sebastián Aguilar
authors.initials: SA
authors.lastname: Pierlé
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA.
authors.forename: Joshua E
authors.initials: JE
authors.lastname: Turse
authors.affiliation: Animal Diseases Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA, USA.
authors.forename: Glen A
authors.initials: GA
authors.lastname: Scoles
authors.affiliation: Environmental Molecular Sciences Laboratory, Fundamental and Computational Sciences, Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Samuel O
authors.initials: SO
authors.lastname: Purvine
authors.affiliation: Biological Sciences Division, Fundamental and Computational Sciences, Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Carrie D
authors.initials: CD
authors.lastname: Nicora
authors.affiliation: Biological Sciences Division, Fundamental and Computational Sciences, Pacific Northwest National Laboratory, Richland, WA, USA.
authors.forename: Therese R W
authors.initials: TR
authors.lastname: Clauss
authors.affiliation: Animal Diseases Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA, USA.
authors.forename: Massaro W
authors.initials: MW
authors.lastname: Ueti
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA. Electronic address: wbrown@vetmed.wsu.edu.
authors.forename: Wendy C
authors.initials: WC
authors.lastname: Brown
authors.affiliation: Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, USA; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA. Electronic address: kbrayton@vetmed.wsu.edu.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Brayton
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D030561:Databases, Nucleic Acid; D003870:Dermacentor / Q000235:genetics* / Q000502:physiology*; D020224:Expressed Sequence Tags; D005247:Feeding Behavior / Q000502:physiology; D020869:Gene Expression Profiling; D040901:Proteomics; D012463:Saliva / Q000737:chemistry*; D012469:Salivary Glands / Q000469:parasitology / Q000502:physiology
chemical_list: D060829:Arthropod Proteins
keywords: Gene expression; Mass spectrometry; Proteomics; Saliva; Salivary proteins; Tick; Transcriptomics