title: Extraction and purification of C-phycocyanin fromSpirulina platensis(CCC540).
abstract: In this study a simple protocol was developed for purifying phycocyanin (PC) fromSpirulina platensis(CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A620/A280). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A620/A280) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD).
authors.affiliation: Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi, 110012 India.
authors.forename: Devendra
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi, 110012 India.
authors.forename: Dolly Wattal
authors.initials: DW
authors.lastname: Dhar
authors.affiliation: Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi, 110012 India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Pabbi
authors.affiliation: Microbiology Department, Kurukshetra University, Kurukshetra, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Division of Agricultural Chemical, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Suresh
authors.initials: S
authors.lastname: Walia
keywords: DEAE-Cellulose-11; Phycocyanin; Spirulina
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