title: ELF-MF attenuates quercetin-induced apoptosis in K562 cells through modulating the expression of Bcl-2 family proteins.
abstract: This study investigated the effects of sinusoidal ELF-MF (1 mT; 50 Hz) on the apoptosis induced by four different compounds, namely vinblastine, etoposide, quercetin, and resveratrol, in human K562 chronic myeloid leukemia cells. The exposure to ELF-MF did not affect growth and viability of untreated K562 cells and did not influence the anti-proliferative effects of resveratrol, vinblastine, and etoposide. On the contrary, in quercetin-treated cells, exposure to ELF-MF significantly reduced the percentage of apoptotic cells and the caspase-3 activity and modified the cell cycle profile especially after 48 h of exposure. In addition, the simultaneous treatments for 24 h with quercetin plus ELF-MF increased Bcl-2 protein expression and prevented quercetin-induced downregulation of Mcl-1 and Bcl-xL. Finally, an increase of HSP70 expression was also observed after prolonged ELF-MF treatment. The ELF-MF-dependent modulation of the expression of anti-apoptotic Bcl-2 family and Hsp70 proteins could act as a pro-survival mechanism in K562 cells.
authors.affiliation: Department of Biotechnological and Applied Clinical Science, University of L'Aquila, Via Vetoio, Coppito 2, 67100, L'Aquila, Italy, fabrizia.brisdelli@cc.univaq.it.
authors.forename: Fabrizia
authors.initials: F
authors.lastname: Brisdelli
authors.forename: Francesca
authors.initials: F
authors.lastname: Bennato
authors.forename: Argante
authors.initials: A
authors.lastname: Bozzi
authors.forename: Benedetta
authors.initials: B
authors.lastname: Cinque
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Mancini
authors.forename: Roberto
authors.initials: R
authors.lastname: Iorio
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D060526:Magnetic Fields; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D011794:Quercetin / Q000494:pharmacology*; D051020:bcl-X Protein / Q000096:biosynthesis*
chemical_list: D000975:Antioxidants; C000595707:BCL2 protein, human; C494811:BCL2L1 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein; D011794:Quercetin; C505407:CASP3 protein, human; D053148:Caspase 3
references.citation: Mol Cell Biochem. 2007 Feb;296(1-2):137-49
references.pmid: 16969687
references.citation: Curr Drug Metab. 2009 Jul;10(6):530-46
references.pmid: 19702538
references.citation: Biochem Biophys Res Commun. 2001 Sep 7;286(5):953-7
references.pmid: 11527392
references.citation: J Cell Physiol. 2009 May;219(2):334-43
references.pmid: 19115234
references.citation: Bioelectromagnetics. 1998;19(2):85-91
references.pmid: 9492164
references.citation: J Nutr Biochem. 2005 Mar;16(3):155-62
references.pmid: 15741050
references.citation: Bioelectromagnetics. 2012 Dec;33(8):641-51
references.pmid: 22535669
references.citation: Radiat Environ Biophys. 2010 Nov;49(4):731-41
references.pmid: 20582429
references.citation: Acta Biol Hung. 2010 Jun;61(2):158-67
references.pmid: 20519170
references.citation: Biochem Biophys Res Commun. 2002 Mar 29;292(2):355-61
references.pmid: 11906169
references.citation: Altern Med Rev. 2011 Jun;16(2):172-94
references.pmid: 21649459
references.citation: Biochem Pharmacol. 2004 Nov 15;68(10):2019-30
references.pmid: 15476673
references.citation: Cancer Res. 1994 Sep 15;54(18):4952-7
references.pmid: 8069862
references.citation: FASEB J. 1999 Jan;13(1):95-102
references.pmid: 9872934
references.citation: Bioelectromagnetics. 1992;13(5):401-11
references.pmid: 1445421
references.citation: Cancer Chemother Pharmacol. 2005 Mar;55(3):251-62
references.pmid: 15538571
references.citation: Br J Dermatol. 2008 Jun;158(6):1189-96
references.pmid: 18410412
references.citation: Biochim Biophys Acta. 2005 Mar 22;1743(1-2):120-9
references.pmid: 15777847
references.citation: Environ Res. 2004 Feb;94(2):145-51
references.pmid: 14757377
references.citation: PLoS One. 2011 Mar 23;6(3):e18021
references.pmid: 21448285
references.citation: Bioelectromagnetics. 1993;14(3):247-55
references.pmid: 8391817
references.citation: J Cell Physiol. 2011 Nov;226(11):2901-7
references.pmid: 21302292
references.citation: Ann N Y Acad Sci. 2006 Dec;1090:59-68
references.pmid: 17384247
references.citation: J Biol Chem. 2005 Mar 18;280(11):10491-500
references.pmid: 15637055
references.citation: Chem Res Toxicol. 1992 Mar-Apr;5(2):227-31
references.pmid: 1322737
references.citation: Annu Rev Physiol. 2008;70:73-91
references.pmid: 17680735
references.citation: Mutat Res. 2010 Jan 5;683(1-2):74-83
references.pmid: 19896957
references.citation: Gen Physiol Biophys. 2012 Mar;31(1):1-10
references.pmid: 22447825
references.citation: Electromagn Biol Med. 2010 Aug;29(3):122-30
references.pmid: 20707646
references.citation: Blood. 2003 Nov 1;102(9):3179-85
references.pmid: 12855558
references.citation: Occup Environ Med. 2007 Aug;64(8):553-9
references.pmid: 17525094
references.citation: Methods Cell Biol. 1995;46:153-85
references.pmid: 7541883
references.citation: Eur J Pharmacol. 2010 Dec 15;649(1-3):84-91
references.pmid: 20858478
references.citation: J Physiol Pharmacol. 2005 Dec;56 Suppl 6:101-8
references.pmid: 16340043
references.citation: Int J Radiat Biol. 2013 Jul;89(7):548-61
references.pmid: 23367877
references.citation: Crit Rev Clin Lab Sci. 2006;43(1):1-67
references.pmid: 16531274
references.citation: J Cancer Res Clin Oncol. 2005 Nov;131(11):765-71
references.pmid: 16049707
references.citation: Cardiology. 2010;117(1):54-6
references.pmid: 20924178
references.citation: J Biomed Biotechnol. 2009;2009:834239
references.pmid: 19672456
references.citation: Int J Radiat Biol. 2005 Jan;81(1):1-11
references.pmid: 15962758
references.citation: J Cell Physiol. 2008 Apr;215(1):129-39
references.pmid: 17941084
references.citation: Biochem Biophys Res Commun. 2010 Oct 1;400(4):739-44
references.pmid: 20816755
references.citation: J Immunol Methods. 2002 Jul 1;265(1-2):97-110
references.pmid: 12072181
references.citation: Bioelectromagnetics. 2009 Feb;30(2):163-5
references.pmid: 19051321
references.citation: Biochem Pharmacol. 2005 May 15;69(10):1421-32
references.pmid: 15857606
references.citation: Bioelectromagnetics. 2011 Jan;32(1):15-27
references.pmid: 20690107