title: [Effect of retinociacdi combined extracts from testudinis carapacis et plastri on proliferating in MSCs and its mechanism].
abstract: OBJECTIVE
  To explore the effect of retinociacdi (RA) combined extracts from Testudinis Carapacis et Plastri(PTE) on proliferating in MSCs and its mechanism.
  
  
  METHODS
  Transfected PGL3-ID1 using the calcium phosphate co-precipitation method in rat MSCs. PTE combined with RA and retinociacdi receptor inhibitor(Ro41) acted on transfected MSCs with respective concentrations of 10(-6), 10(-7) and 10(-8) mol/L. Luciferase activity measurement was used to detect the activity of RAR and IDI 36 h later. PTE acted on MSCs 36 h,3 d and 7 d for respective concentrations of 1, 3, 30 and 100 microg/mL,then collected cells to detect RAR with RT-PCR. PTE combined with RA for 10(-7) mol/L and Ro41 for 10(-6) mol/L respectively on MSCs for 36 h,and then collected cells to detect RAR and ID1 with RT-PCR.
  
  
  RESULTS
  PTE promoted expression of ID1 on MSCs. When combined with RA, the promotion effect became greater and it promoted expression of RAR at the same time; When inhibited RA using Ro41, the promotion of IDI was weaken by PTE.
  
  
  CONCLUSIONS
  RA promotes expression of IDI on MSCs, PTE regulates proliferation and differentiation of MSCs by expression of nuclear receptor RAR.
authors.forename: Hai-ling
authors.initials: HL
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Yin
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Jin-feng
authors.initials: JF
authors.lastname: Chen
authors.forename: Dong-feng
authors.initials: DF
authors.lastname: Chen
authors.forename: Zi-chun
authors.initials: ZC
authors.lastname: Hua
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics / Q000378:metabolism*; D008419:Materia Medica / Q000008:administration & dosage / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018168:Receptors, Retinoic Acid / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014020:Tissue Extracts / Q000494:pharmacology; D014162:Transfection; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology*; D014426:Turtles
chemical_list: C497454:ID1 protein, rat; D051798:Inhibitor of Differentiation Protein 1; D008419:Materia Medica; D018168:Receptors, Retinoic Acid; D014020:Tissue Extracts; D014212:Tretinoin