title: Functional analysis of Drosophila HSP70 promoter with different HSE numbers in human cells.
abstract: The activation of genetic constructs including the Drosophila hsp70 promoter with four and eight HSE sequences in the regulatory region has been described in human cells. The promoter was shown to be induced at lower temperatures compared to the human hsp70 promoter. The promoter activity increased after a 60-min heat shock already at 38 °C in human cells. The promoter activation was observed 24 h after heat shock for the constructs with eight HSEs, while those with four HSEs required 48 h. After transplantation of in vitro heat-shocked transfected cells, the promoter activity could be maintained for 3 days with a gradual decline. The promoter activation was confirmed in vivo without preliminary heat shock in mouse ischemic brain foci. Controlled expression of the Gdnf gene under a Drosophila hsp70 promoter was demonstrated. This promoter with four and eight HSE sequences in the regulatory region can be proposed as a regulated promoter in genetic therapeutic systems.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia; Ltd Apto-pharm, Moscow, Russia.
authors.forename: Nadezda
authors.initials: N
authors.lastname: Kust
authors.affiliation: Ltd Apto-pharm, Moscow, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Rybalkina
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Ilya
authors.initials: I
authors.lastname: Mertsalov
authors.affiliation: Institute of Medicine and Cell Transplantation, Moscow, Russia; Ltd Apto-pharm, Moscow, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Savchenko
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia; Institute of Medicine and Cell Transplantation, Moscow, Russia; Ltd Apto-pharm, Moscow, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Revishchin
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia; Institute of Medicine and Cell Transplantation, Moscow, Russia; Ltd Apto-pharm, Moscow, Russia.
authors.forename: Gali
authors.initials: G
authors.lastname: Pavlova
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D004330:Drosophila / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013696:Temperature; D014162:Transfection
chemical_list: C495039:GDNF protein, human; D051100:Glial Cell Line-Derived Neurotrophic Factor; D018840:HSP70 Heat-Shock Proteins
references.citation: Mol Ther. 2007 Sep;15(9):1630-9
references.pmid: 17609656
references.citation: J Biol Chem. 1984 Dec 10;259(23):14812-7
references.pmid: 6094575
references.citation: Hum Gene Ther. 2002 Sep 1;13(13):1611-20
references.pmid: 12228016
references.citation: Bull Exp Biol Med. 2005 Apr;139(4):514-6
references.pmid: 16027892
references.citation: J Cell Biol. 1986 Aug;103(2):321-5
references.pmid: 2426277
references.citation: J Virol. 2006 Jan;80(1):27-37
references.pmid: 16352528
references.citation: Adv Drug Deliv Rev. 2009 Jul 2;61(7-8):527-41
references.pmid: 19394373
references.citation: Nucleic Acids Res. 1986 Oct 24;14(20):8183-8
references.pmid: 3095794
references.citation: Science. 1988 Mar 4;239(4844):1139-42
references.pmid: 3125608
references.citation: Mol Cell Biol. 1991 Jan;11(1):586-92
references.pmid: 1986252
references.citation: Cell Stress Chaperones. 2007 Autumn;12(3):283-90
references.pmid: 17915561
references.citation: PLoS One. 2010 May 12;5(5):e10611
references.pmid: 20485554
references.citation: Genetika. 2000 Nov;36(11):1436-42
references.pmid: 11094760
references.citation: Neurosci Lett. 1985 Nov 11;61(3):321-6
references.pmid: 3909005
references.citation: Gene. 1988 Nov 15;71(1):207-10
references.pmid: 2850976
references.citation: Proc Natl Acad Sci U S A. 1981 Nov;78(11):7038-42
references.pmid: 6273904
references.citation: Adv Genet. 1987;24:31-72
references.pmid: 3324701
references.citation: Genetika. 2008 Jul;44(7):992-9
references.pmid: 18767548
references.citation: J Virol. 2002 Nov;76(22):11605-11
references.pmid: 12388721
references.citation: Annu Rev Cell Dev Biol. 1995;11:441-69
references.pmid: 8689565
references.citation: J Cereb Blood Flow Metab. 1986 Aug;6(4):505-10
references.pmid: 3733910
references.citation: EMBO J. 1982;1(11):1473-7
references.pmid: 6327271
references.citation: Mol Cell Biol. 1989 Apr;9(4):1746-53
references.pmid: 2471067
references.citation: Mol Cell Biol. 1988 Sep;8(9):3761-9
references.pmid: 3146692
references.citation: Cell. 1991 Feb 8;64(3):585-93
references.pmid: 1899357