title: Natural vertical transmission of dengue-1 virus in Aedes aegypti populations in Acapulco, Mexico.
abstract: We carried out dengue virus surveillance in Aedes aegypti populations from 47 neighborhoods of Acapulco during the rainy season of 2011 following a standard national protocol and as an improvement of the entomological surveillance of the Mexican Dengue Control Program. A total of 4,146 Ae. aegypti adults collected indoors and/or emerged from eggs, larvae, or pupae from households with dengue reports (probable or confirmed cases), were grouped into pools and processed using a standardized serotype-specific 4-plex real-time reverse transcriptase-polymerase chain reaction assay. Overall, only 2 (0.9%) of 226 pools of Ae. aegypti adults (1 pool of adults emerged from field-collected larvae, and another of indoor-collected adults) were positive for dengue virus 1 (DENV-1). This is appears to be the 1st report of evidence on the vertical and transovarial transmission of DENV-1 in field-caught Ae. aegypti in Mexico.
authors.forename: Norma E
authors.initials: NE
authors.lastname: Martínez
authors.forename: Felipe
authors.initials: F
authors.lastname: Dzul-Manzanilla
authors.forename: Cipriano
authors.initials: C
authors.lastname: Gutiérrez-Castro
authors.forename: Jesus
authors.initials: J
authors.lastname: Ibarra-López
authors.forename: Wilbert
authors.initials: W
authors.lastname: Bibiano-Marín
authors.forename: Leonardo
authors.initials: L
authors.lastname: López-Damián
authors.forename: Andrés
authors.initials: A
authors.lastname: Martini-Jaimes
authors.forename: Herón
authors.initials: H
authors.lastname: Huerta
authors.forename: Azael
authors.initials: A
authors.lastname: Che-Mendoza
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Ayora-Talavera
authors.forename: Pablo
authors.initials: P
authors.lastname: Manrique-Saide
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D003715:Dengue / Q000635:transmission*; D003716:Dengue Virus / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D008800:Mexico; D020133:Reverse Transcriptase Polymerase Chain Reaction