title: Novel functional changes during podocyte differentiation: increase of oxidative resistance and H-ferritin expression.
abstract: Podocytes are highly specialized, arborized epithelial cells covering the outer surface of the glomerular tuft in the kidney. Terminally differentiated podocytes are unable to go through cell division and hereby they are lacking a key property for regeneration after a toxic injury. Podocytes are long-lived cells but, to date, little is known about the mechanisms that support their stress resistance. Our aim was to investigate whether the well-known morphological changes during podocyte differentiation are accompanied by changes in oxidative resistance in a manner that could support their long-term survival. We used a conditionally immortalized human podocyte cell line to study the morphological and functional changes during differentiation. We followed the differentiation process for 14 days by time-lapse microscopy. During this period nondifferentiated podocytes gradually transformed into large, nonproliferating, frequently multinucleated cells, with enlarged nuclei and opened chromatin structure. We observed that differentiated podocytes were highly resistant to oxidants such as H2O2 and heme when applied separately or in combination, whereas undifferentiated cells were prone to such challenges. Elevated oxidative resistance of differentiated podocytes was associated with increased activities of antioxidant enzymes and H-ferritin expression. Immunohistochemical analysis of normal human kidney specimens revealed that podocytes highly express H-ferritin in vivo as well.
authors.affiliation: Department of Medicine, University of Debrecen, Debrecen 4032, Hungary.
authors.forename: Emese
authors.identifier: 0000-0001-7873-1864
authors.initials: E
authors.lastname: Bányai
authors.affiliation: Department of Medicine, University of Debrecen, Debrecen 4032, Hungary.
authors.forename: Enikő
authors.initials: E
authors.lastname: Balogh
authors.affiliation: Division of Clinical Physiology, Institute of Cardiology, University of Debrecen, Debrecen 4032, Hungary.
authors.forename: Miklós
authors.identifier: 0000-0003-3262-884X
authors.initials: M
authors.lastname: Fagyas
authors.affiliation: Department of Molecular and Translational Medicine, University of Brescia, 25125 Brescia, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Arosio
authors.affiliation: MTA-DE Vascular Biology, Thrombosis and Hemostasis Research Group, Hungarian Academy of Sciences, Debrecen 4032, Hungary.
authors.forename: Zoltán
authors.identifier: 0000-0001-9967-5005
authors.initials: Z
authors.lastname: Hendrik
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen 4010, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Király
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen 4010, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Nagy
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen 4010, Hungary.
authors.forename: Bence
authors.initials: B
authors.lastname: Tánczos
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen 4010, Hungary.
authors.forename: István
authors.identifier: 0000-0003-2692-6453
authors.initials: I
authors.lastname: Pócsi
authors.affiliation: MTA-DE Vascular Biology, Thrombosis and Hemostasis Research Group, Hungarian Academy of Sciences, Debrecen 4032, Hungary ; Department of Pediatrics, University of Debrecen, Debrecen 4032, Hungary.
authors.forename: György
authors.initials: G
authors.lastname: Balla
authors.affiliation: Department of Medicine, University of Debrecen, Debrecen 4032, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Balla
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen 4010, Hungary.
authors.forename: Gáspár
authors.initials: G
authors.lastname: Bánfalvi
authors.affiliation: Department of Medicine, University of Debrecen, Debrecen 4032, Hungary ; MTA-DE Vascular Biology, Thrombosis and Hemostasis Research Group, Hungarian Academy of Sciences, Debrecen 4032, Hungary.
authors.forename: Viktória
authors.identifier: 0000-0003-4942-7091
authors.initials: V
authors.lastname: Jeney
mesh_terms: D001052:Apoferritins / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D018715:Microscopy, Video; D018384:Oxidative Stress; D050199:Podocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D059008:Time-Lapse Imaging
chemical_list: D002843:Chromatin; D001052:Apoferritins; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
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