title: Acinetobacter refrigeratoris [corrected] sp. nov., isolated from a domestic refrigerator.
abstract: A Gram-negative bacterial strain, designated WB1(T), was isolated from a domestic refrigerator in Guangzhou, PR China. Cells of strain WB1(T) were oxidase-negative, catalase-positive, strictly aerobic, non-spore-forming and non-motile coccobacilli with peritrichous fimbriae-like structures. The strain was able to grow at 10-40 °C with optimum growth at 28-30 °C, pH 6.0-8.0 (optimum, pH 7.0) and 0-6 % NaCl (w/v, optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene and rpoB gene sequences revealed that strain WB1(T) belonged to the genus Acinetobacter and was most closely related to A. indicus DSM 25388(T) (97.2 % 16S rRNA gene sequence similarity) and A. radioresistens NBRC 102413(T) (96.8 %). The DNA G + C content of strain WB1(T) was 46.74 ± 0.04 mol % and the major fatty acids comprised summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C18:1 ω9c, C16:0 and C12:0. The predominant respiratory quinone was identified as Q-9 and the polar lipids as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and an unidentified phospholipid. Phenotypic, phylogenetic and chemotaxonomic data, including low DNA-DNA relatedness with closely related type strains, supported that strain WB1(T) represents a distinct novel species in the genus Acinetobacter, for which the name Acinetobacter refrigeratorensis sp. nov. was proposed. The type strain is WB1(T) (=GIMCC 1.663(T) = CCTCC AB 2014197(T) = KCTC 42011(T)).
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Building 59th, 100 Xianlie Zhong Road, Guangzhou, 510070, China.
authors.forename: Guangda
authors.initials: G
authors.lastname: Feng
authors.forename: Songzhen
authors.initials: S
authors.lastname: Yang
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Yao
authors.forename: Honghui
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000332:Aerobiosis; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012034:Refrigeration; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit
references.citation: Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):716-721
references.pmid: 22140171
references.citation: Syst Appl Microbiol. 1983;4(2):184-92
references.pmid: 23194591
references.citation: J Bacteriol. 1991 Jan;173(2):697-703
references.pmid: 1987160
references.citation: Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4402-4406
references.pmid: 23950148
references.citation: Res Microbiol. 2011 May;162(4):393-404
references.pmid: 21320596
references.citation: Eur J Biochem. 1970 Jan;12(1):133-42
references.pmid: 4984993
references.citation: J Clin Microbiol. 2006 Mar;44(3):827-32
references.pmid: 16517861
references.citation: Appl Environ Microbiol. 1982 Oct;44(4):992-3
references.pmid: 6184019
references.citation: Int J Syst Evol Microbiol. 2003 Sep;53(Pt 5):1563-1567
references.pmid: 13130049
references.citation: Ann Inst Pasteur (Paris). 1954 Jun;86(6):722-8
references.pmid: 13197842
references.citation: Evolution. 1985 Jul;39(4):783-791
references.pmid: 28561359
references.citation: Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):953-963
references.pmid: 12892111
references.citation: Int J Syst Evol Microbiol. 2013 Aug;63(Pt 8):2963-2969
references.pmid: 23396716
references.citation: Int J Syst Evol Microbiol. 2011 Dec;61(Pt 12):2837-2843
references.pmid: 21239566
references.citation: J Mol Evol. 1981;17(6):368-76
references.pmid: 7288891
references.citation: Int J Syst Evol Microbiol. 2009 Jan;59(Pt 1):118-24
references.pmid: 19126734
references.citation: Appl Microbiol Biotechnol. 2013 Jan;97(1):329-40
references.pmid: 22669636
references.citation: J Gen Microbiol. 1977 Jun;100(2):221-30
references.pmid: 894261
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1532-1539
references.pmid: 22904213
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: Int J Syst Evol Microbiol. 2014 Mar;64(Pt 3):1043-1050
references.pmid: 24363297