title: Characterization of recombinant malarial RecQ DNA helicase.
abstract: RecQ DNA gene of multi-drug resistant Plasmodium falciparum K1 (PfRecQ1) was cloned, and the recombinant C-terminal-decahistidine-tagged PfRecQ1 was expressed in Escherichia coli. The purified enzyme could efficiently unwind partial duplex DNA substrate in a 3' to 5' direction. The malarial RecQ1 could not unwind substrates with both 5' and 3' overhangs, those with a 5' overhang, or blunt-ended DNA duplexes. Unwinding of DNA helicase activity was driven by the hydrolysis of ATP. The drug inhibitory effects of six compounds indicated that only doxorubicin and daunorubicin could inhibit the unwinding activity.
authors.affiliation: Faculty of Science, Rangsit University, Thailand.
authors.forename: Pattra
authors.initials: P
authors.lastname: Suntornthiticharoen
authors.affiliation: Molecular Biotechnology Laboratory, Suranaree University of Technology, Thailand.
authors.forename: Witsanu
authors.initials: W
authors.lastname: Srila
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Thailand.
authors.forename: Porntip
authors.initials: P
authors.lastname: Chavalitshewinkoon-Petmitr
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Thailand.
authors.forename: Paviga
authors.initials: P
authors.lastname: Limudomporn
authors.affiliation: Molecular Biotechnology Laboratory, Suranaree University of Technology, Thailand. Electronic address: montarop@sut.ac.th.
authors.forename: Montarop
authors.initials: M
authors.lastname: Yamabhai
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004791:Enzyme Inhibitors / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006868:Hydrolysis; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000235:genetics; D011487:Protein Conformation; D053484:RecQ Helicases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
chemical_list: D016054:DNA, Protozoan; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D004247:DNA; D053484:RecQ Helicases
keywords: Cloning; DNA helicase; Expression; Malaria; Plasmodium falciparum; RecQ