title: Type 2C protein phosphatase is a key regulator of antiviral extreme resistance limiting virus spread.
abstract: Effector-triggered immunity (ETI) is an active immune response triggered by interactions between host resistance proteins and their cognate effectors. Although ETI is often associated with the hypersensitive response (HR), various R genes mediate an HR-independent process known as extreme resistance (ER). In the soybean-Soybean mosaic virus (SMV) pathosystem, the strain-specific CI protein of SMV functions as an effector of Rsv3-mediated ER. In this study, we used the soybean (Rsv3)-SMV (CI) pathosystem to gain insight into the molecular signaling pathway involved in ER. We used genome-wide transcriptome analysis to identify a subset of the type 2C protein phophatase (PP2C) genes that are specifically up-regulated in Rsv3-mediated ER. Gain-of-function analysis of the most significantly expressed soybean PP2C gene, GmPP2C3a, showed that ABA-induced GmPP2C3a functions as a key regulator of Rsv3-mediated ER. Our results further suggest that the primary mechanism of ER against viruses is the inhibition of viral cell-to-cell movement by callose deposition in an ABA signaling-dependent manner.
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
authors.affiliation: Horticultural and Crop Herbal Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 440-310, Republic of Korea.
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Kwon
authors.affiliation: Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Cho
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D060467:Disease Resistance; D017343:Genes, Plant; D054884:Host-Pathogen Interactions; D009029:Mosaic Viruses / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000502:physiology*; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000821:virology; D010940:Plant Proteins / Q000502:physiology*; D000071636:Protein Phosphatase 2C; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics / Q000821:virology; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C
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