title: Clinical relevance of IL-10 gene polymorphism in patients with major trauma.
abstract: OBJECTIVE
  To assess IL-10 serum concentration according to outcome of severe trauma treatment and influence of short nuclear polymorphism (SNP) 1082G/A within IL-10 gene on treatment outcome of patients with severe trauma.
  
  
  METHODS
  Forty-seven patients with major trauma were prospectively recruited, and they were divided into two groups according to outcome (survivors and non-survivors). The IL-10 gene polymorphisms were genotyped using restriction fragment length polymorphism analysis. Serum IL-10 levels were determined with enzyme-linked immunosorbent assay. Association between IL-10 serum concentration, IL-10 SNP type and IL-10 serum concentration in groups of patients with different SNPs with outcome after severe trauma was evaluated.
  
  
  RESULTS
  Mean age of patients was 35.53±14.53 years. The major mechanism of injury was traffic, and the mean injury severity score was 35.47±11.23. Despite higher values of IL-10 serum concentrations in patients with lethal outcome, the difference was not statistically significant. In 40 (85%) patients no gene polymorphism for IL-10 was recorded. No statistical significance in frequency of IL-10-1082 gene polymorphism was observed between the patients with different outcomes of polytrauma. No statistically significant difference in IL-10 values was evidenced between the subjects with and without polymorphisms in any of the observed times of measurement, although a trend toward the higher values may be observed in patients with polymorphism in heterozygous form.
  
  
  CONCLUSIONS
  The patients with IL-10 SNP gene polymorphism despite no proven statistical significance appeared to have higher values of IL-10 and consequently worse outcome.
authors.affiliation: School of Medicine, University of Belgrade, 2Clinic for Emergency Surgery, 3Clinic for Gastroenterology and Hepatology, 4Center for Anesthesiology and Reanimation; Clinical Center of Serbia; Belgrade, Serbia.
authors.forename: Vasilije
authors.initials: V
authors.lastname: Jeremić
authors.forename: Tamara
authors.initials: T
authors.lastname: Alempijević
authors.forename: Srđan
authors.initials: S
authors.lastname: Mijatović
authors.forename: Vladimir
authors.initials: V
authors.lastname: Arsenijević
authors.forename: Nebojša
authors.initials: N
authors.lastname: Ladjevic
authors.forename: Slobodan
authors.initials: S
authors.lastname: Krstić
mesh_terms: D000328:Adult; D005260:Female; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000097:blood* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D011446:Prospective Studies; D018709:Statistics, Nonparametric; D014947:Wounds and Injuries / Q000097:blood* / Q000235:genetics*
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10