title: Synthesis of mixed-sequence oligonucleotides on mesoporous silicon: chemical strategies and material stability.
abstract: Rapid screening tests in medical diagnostic and environmental analysis are often based on oligonucleotide biochips. In this paper, we studied the stability of functionalized mesoporous silicon supports in the solid-phase synthesis of oligonucleotides, exploiting several chemical procedures. A 19-mer mixed sequence has been successfully synthesized on aminosilane-modified porous silicon photonic structures. The process and the materials have been characterized by optical reflectivity, atomic force microscopy and high-performance liquid chromatography.
authors.affiliation: Institute for Microelectronics and Microsystems, National Council of Research, Naples 80131, Italy ; Department of Pharmacy, University of Naples Federico II, Naples 80138, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Terracciano
authors.affiliation: Institute for Microelectronics and Microsystems, National Council of Research, Naples 80131, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Rea
authors.affiliation: Institute for Microelectronics and Microsystems, National Council of Research, Naples 80131, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: De Stefano
authors.affiliation: Institute for Microelectronics and Microsystems, National Council of Research, Naples 80131, Italy.
authors.forename: Ivo
authors.initials: I
authors.lastname: Rendina
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples 80138, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Oliviero
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples 80138, Italy.
authors.forename: Fabrizia
authors.initials: F
authors.lastname: Nici
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples 80138, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: D'Errico
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples 80138, Italy.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Piccialli
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples 80138, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Borbone
keywords: DNA synthesis; Deprotection conditions; Mesoporous silicon functionalization; Surface stability
references.citation: Annu Rev Biomed Eng. 2002;4:129-53
references.pmid: 12117754
references.citation: Curr Protoc Mol Biol. 2001 May;Chapter 2:Unit2.11
references.pmid: 18265179
references.citation: J R Soc Interface. 2013 Mar 27;10(83):20130160
references.pmid: 23536541
references.citation: Sensors (Basel). 2008 Oct 23;8(10):6549-6556
references.pmid: 27873885