title: Lack of association between miR-27a, miR-146a, miR-196a-2, miR-492 and miR-608 gene polymorphisms and colorectal cancer.
abstract: Colorectal cancer (CRC) is one of the most common cancers worldwide with high mortality rates. MicroRNAs (miRNAs) have an established role in the development of different cancers. Single nucleotide polymorphisms (SNPs) in miRNA related genes were linked with various gastrointestinal malignancies. However, the data on association between miRNA SNPs and CRC development are inconsistent. The aim of the present study was to evaluate the association between miRNA-related gene polymorphisms (miR-27a, miR-146a, miR-196a-2, miR-492 and miR-608) and the presence of CRC in European population. Gene polymorphisms were analyzed in 621 subjects (controls: n = 428; CRC: n = 193). MiR-27a T>C (rs895819), miR-146a G>C (rs2910164), miR-196a-2 C>T (rs11614913), miR-492 G>C (rs2289030) and miR-608 C>G (rs4919510) SNPs were genotyped by RT-PCR. Overall, all genotypes and alleles of miRNA SNPs were distributed equally between control and CRC groups. We observed a tendency for miR-146a C allele to be associated with lower risk of CRC when compared to G allele, however, the difference did not reach the adjusted P-value (odds ratio (OR) = 0.68, 95% confidence interval (CI) 0.49-0.95, P = 0.025). In conclusion, gene polymorphisms of miR-27a, miR-146a, miR-196a-2, miR-492, miR-492a and miR-608 were not associated with the presence of CRC in European subjects.
authors.affiliation: 1] Institute for Digestive Research, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania [2] Department of Gastroenterology, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Juozas
authors.initials: J
authors.lastname: Kupcinskas
authors.affiliation: Institute for Digestive Research, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Indre
authors.initials: I
authors.lastname: Bruzaite
authors.affiliation: Institute for Digestive Research, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Simonas
authors.initials: S
authors.lastname: Juzenas
authors.affiliation: Institute for Digestive Research, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Ugne
authors.initials: U
authors.lastname: Gyvyte
authors.affiliation: 1] Institute for Digestive Research, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania [2] Department of Gastroenterology, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Laimas
authors.initials: L
authors.lastname: Jonaitis
authors.affiliation: 1] Institute for Digestive Research, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania [2] Department of Gastroenterology, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Gediminas
authors.initials: G
authors.lastname: Kiudelis
authors.affiliation: Institute for Digestive Research, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Jurgita
authors.initials: J
authors.lastname: Skieceviciene
authors.affiliation: Faculty of Medicine, University of Latvia, Raina Bulvaris 19, LV-1050, Riga, Latvia.
authors.forename: Marcis
authors.initials: M
authors.lastname: Leja
authors.affiliation: Department of Surgery, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Henrikas
authors.initials: H
authors.lastname: Pauzas
authors.affiliation: Department of Surgery, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Algimantas
authors.initials: A
authors.lastname: Tamelis
authors.affiliation: Department of Surgery, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Dainius
authors.initials: D
authors.lastname: Pavalkis
authors.affiliation: 1] Institute for Digestive Research, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania [2] Department of Gastroenterology, Lithuanian University of Health Sciences, Eiveniu str. 2, LT-50009, Kaunas, Lithuania.
authors.forename: Limas
authors.initials: L
authors.lastname: Kupcinskas
mesh_terms: D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D044465:White People / Q000235:genetics
chemical_list: D035683:MicroRNAs
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