title: Whole-genome analysis of muscle founder cells implicates the chromatin regulator Sin3A in muscle identity.
abstract: Skeletal muscles are formed in numerous shapes and sizes, and this diversity impacts function and disease susceptibility. To understand how muscle diversity is generated, we performed gene expression profiling of two muscle subsets from Drosophila embryos. By comparing the transcriptional profiles of these subsets, we identified a core group of founder cell-enriched genes. We screened mutants for muscle defects and identified functions for Sin3A and 10 other transcription and chromatin regulators in the Drosophila embryonic somatic musculature. Sin3A is required for the morphogenesis of a muscle subset, and Sin3A mutants display muscle loss and misattachment. Additionally, misexpression of identity gene transcription factors in Sin3A heterozygous embryos leads to direct transformations of one muscle into another, whereas overexpression of Sin3A results in the reverse transformation. Our data implicate Sin3A as a key buffer controlling muscle responsiveness to transcription factors in the formation of muscle identity, thereby generating tissue diversity.
authors.affiliation: Program in Developmental Biology, Sloan-Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Krista C
authors.initials: KC
authors.lastname: Dobi
authors.affiliation: Department of Biochemistry, State University of New York at Buffalo, Buffalo, NY 14203, USA.
authors.forename: Marc S
authors.initials: MS
authors.lastname: Halfon
authors.affiliation: Program in Developmental Biology, Sloan-Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address: m-baylies@ski.mskcc.org.
authors.forename: Mary K
authors.initials: MK
authors.lastname: Baylies
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002843:Chromatin / Q000235:genetics*; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D049750:Genome, Insect; D018482:Muscle, Skeletal / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D056264:Sin3 Histone Deacetylase and Corepressor Complex
chemical_list: D002843:Chromatin; D012097:Repressor Proteins; C099683:SIN3A transcription factor; D056264:Sin3 Histone Deacetylase and Corepressor Complex
references.citation: Int Rev Neurobiol. 2006;75:55-70
references.pmid: 17137923
references.citation: Curr Genet. 2005 Jan;47(1):1-17
references.pmid: 15565322
references.citation: Nucleic Acids Res. 2002 Jan 1;30(1):207-10
references.pmid: 11752295
references.citation: Curr Opin Genet Dev. 2001 Aug;11(4):431-9
references.pmid: 11448630
references.citation: Development. 1995 Jul;121(7):1979-88
references.pmid: 7635046
references.citation: Exp Cell Res. 2010 Nov 1;316(18):3019-27
references.pmid: 20673829
references.citation: Genes Dev. 1990 Dec;4(12A):2098-111
references.pmid: 1980118
references.citation: Development. 2001 Nov;128(21):4251-64
references.pmid: 11684661
references.citation: Pharmacol Res. 2010 Jul;62(1):1-2
references.pmid: 20471557
references.citation: EMBO Rep. 2001 Mar;2(3):211-6
references.pmid: 11266362
references.citation: Curr Opin Genet Dev. 2008 Oct;18(5):404-10
references.pmid: 18929655
references.citation: Cell. 1998 Jun 12;93(6):921-7
references.pmid: 9635422
references.citation: Genetics. 2013 May;194(1):189-98
references.pmid: 23457234
references.citation: Genome Biol. 2002;3(12):RESEARCH0088
references.pmid: 12537577
references.citation: Nat Protoc. 2009;4(1):44-57
references.pmid: 19131956
references.citation: Genome Res. 2002 Aug;12(8):1294-300
references.pmid: 12176937
references.citation: Mol Cell Biochem. 2012 Jul;366(1-2):251-8
references.pmid: 22476904
references.citation: Trends Genet. 1999 Nov;15(11):448-53
references.pmid: 10529807
references.citation: Genes Dev. 2013 Jun 15;27(12):1391-405
references.pmid: 23788625
references.citation: Neuron. 1992 Sep;9(3):549-61
references.pmid: 1524829
references.citation: Semin Neurol. 2008 Apr;28(2):250-9
references.pmid: 18351526
references.citation: Biochem Biophys Res Commun. 1997 Dec 18;241(2):334-40
references.pmid: 9425272
references.citation: PLoS Genet. 2011 Jul;7(7):e1002186
references.pmid: 21811416
references.citation: Genes Dev. 2000 Nov 15;14(22):2906-17
references.pmid: 11090137
references.citation: Genetics. 2000 Oct;156(2):645-63
references.pmid: 11014813
references.citation: Genes Dev. 2011 Jul 15;25(14):1499-509
references.pmid: 21764853
references.citation: Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5662-6
references.pmid: 8202544
references.citation: Development. 2007 Dec;134(24):4357-67
references.pmid: 18003739
references.citation: Development. 2005 Mar;132(6):1211-22
references.pmid: 15703278
references.citation: Mol Cell Biol. 2010 Dec;30(24):5686-97
references.pmid: 20956564
references.citation: Mech Dev. 1995 Mar;50(1):29-41
references.pmid: 7605749
references.citation: Dev Biol. 2010 May 1;341(1):66-83
references.pmid: 19932206
references.citation: Dev Cell. 2012 Apr 17;22(4):721-35
references.pmid: 22445365
references.citation: Development. 2007 Nov;134(22):3985-97
references.pmid: 17942482
references.citation: PLoS Genet. 2011 Jun;7(6):e1002109
references.pmid: 21698137
references.citation: Biochim Biophys Acta. 2009 Jun-Aug;1789(6-8):443-50
references.pmid: 19505602
references.citation: Development. 2012 Mar;139(6):1164-74
references.pmid: 22296846
references.citation: Dev Genes Evol. 1998 Nov;208(9):531-6
references.pmid: 9799435
references.citation: BMC Dev Biol. 2007 Jul 10;7:83
references.pmid: 17623094
references.citation: Curr Top Dev Biol. 2011;96:57-83
references.pmid: 21621067
references.citation: Nature. 2009 Nov 5;462(7269):65-70
references.pmid: 19890324
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7520-4
references.pmid: 8052612
references.citation: Science. 1996 Jun 7;272(5267):1481-4
references.pmid: 8633240
references.citation: Methods Mol Biol. 2008;475:299-314
references.pmid: 18979251
references.citation: Nucleic Acids Res. 2009 Jan;37(1):1-13
references.pmid: 19033363
references.citation: Dev Cell. 2007 May;12(5):751-66
references.pmid: 17488626
references.citation: J Mol Biol. 1999 Aug 6;291(1):71-82
references.pmid: 10438607
references.citation: PLoS Genet. 2011 Oct;7(10):e1002339
references.pmid: 22028675
references.citation: Genes Dev. 2008 Oct 15;22(20):2799-810
references.pmid: 18923078
references.citation: Development. 1993 Jun;118(2):401-15
references.pmid: 8223268
references.citation: Neuron. 1993 Mar;10(3):409-26
references.pmid: 8461134
references.citation: Development. 1997 Sep;124(17):3407-14
references.pmid: 9310335
references.citation: Development. 2003 Dec;130(25):6257-72
references.pmid: 14602676
references.citation: PLoS Genet. 2006 Feb;2(2):e16
references.pmid: 16482229
references.citation: Nature. 2010 Mar 11;464(7286):287-91
references.pmid: 20220848
references.citation: Mol Cell. 2008 Nov 7;32(3):359-70
references.pmid: 18995834
references.citation: Development. 2002 Mar;129(5):1119-29
references.pmid: 11874908
references.citation: Fly (Austin). 2011 Apr-Jun;5(2):68-75
references.pmid: 21339707
references.citation: Dev Dyn. 2008 Oct;237(10):3040-50
references.pmid: 18816856
references.citation: Nature. 2012 Mar 18;484(7392):120-4
references.pmid: 22425998
references.citation: Science. 2011 Jan 21;331(6015):304-8
references.pmid: 21148348
references.citation: J Biol Chem. 2010 Aug 27;285(35):27457-27467
references.pmid: 20566628