title: Purification of dorsal root ganglion neurons from rat by immunopanning.
abstract: Dorsal root ganglion neurons (DRGs) are sensory neurons that facilitate somatosensation and have been used to study neurite outgrowth, regeneration, and degeneration and PNS and CNS myelination. Studies of DRGs have relied on cell isolation strategies that generally involve extended culture in the presence of antimitotic agents or other cytotoxic treatments that target dividing cells. The surviving cells typically are dependent on serum for growth. Other methods, involving purification of DRGs based on their large size, produce low yield. In contrast, the immunopanning-based method described here for prospective isolation of DRGs from rodents allows for rapid purification in the absence of antimitotic agents and serum. These DRG cultures take place in a defined medium. They are free of Schwann cells and other glia and thus can be used to study the role of glia in the biology of DRG neurons.
authors.affiliation: Department of Neurobiology, School of Medicine, Stanford University, Stanford, California 94305.
authors.forename: J Bradley
authors.initials: JB
authors.lastname: Zuchero
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D016895:Culture Media, Serum-Free / Q000737:chemistry; D005727:Ganglia, Spinal / Q000166:cytology*; D007158:Immunologic Techniques / Q000379:methods*; D009474:Neurons / Q000502:physiology*; D051381:Rats
chemical_list: D016895:Culture Media, Serum-Free
references.citation: J Neurosci Methods. 2008 Jun 30;171(2):239-47
references.pmid: 18471889
references.citation: Cold Spring Harb Protoc. 2014 Aug 01;2014(8):813-4
references.pmid: 25086024