title: Chikungunya virus was isolated in Thailand, 2010.
abstract: Chikungunya fever (CHIKF) is an acute febrile illness caused by a mosquito-borne alphavirus, chikungunya virus (CHIKV). This disease re-emerged in Kenya in 2004, and spread to the countries in and around the Indian Ocean. The re-emerging epidemics rapidly spread to regions like India and Southeast Asia, and it was subsequently identified in Europe in 2007, probably as a result of importation of chikungunya cases. On the one hand, chikungunya is one of the neglected diseases and has only attracted strong attention during large outbreaks. In 2008-2009, there was a major outbreak of chikungunya fever in Thailand, resulting in the highest number of infections in any country in the region. However, no update of CHIKV circulating in Thailand has been published since 2009. In this study, we examined the viral growth kinetics and sequences of the structural genes derived from CHIKV clinical isolates obtained from the serum specimens of CHIKF-suspected patients in Central Thailand in 2010. We identified the CHIKV harboring two mutations E1-A226V and E2-I211T, indicating that the East, Central, and South African lineage of CHIKV was continuously circulating as an indigenous population in Thailand.
authors.affiliation: Mahidol-Osaka Center for Infectious Diseases, Ratchathewi, Bangkok, 10400, Thailand.
authors.forename: Mikiko
authors.initials: M
authors.lastname: Sasayama
authors.forename: Surachet
authors.initials: S
authors.lastname: Benjathummarak
authors.forename: Norihito
authors.initials: N
authors.lastname: Kawashita
authors.forename: Prasert
authors.initials: P
authors.lastname: Rukmanee
authors.forename: Suntaree
authors.initials: S
authors.lastname: Sangmukdanun
authors.forename: Promsin
authors.initials: P
authors.lastname: Masrinoul
authors.forename: Pannamthip
authors.initials: P
authors.lastname: Pitaksajjakul
authors.forename: Orapim
authors.initials: O
authors.lastname: Puiprom
authors.forename: Pitak
authors.initials: P
authors.lastname: Wuthisen
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kurosu
authors.forename: Panjaporn
authors.initials: P
authors.lastname: Chaichana
authors.forename: Pannamas
authors.initials: P
authors.lastname: Maneekan
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ikuta
authors.forename: Pongrama
authors.initials: P
authors.lastname: Ramasoota
authors.forename: Tamaki
authors.initials: T
authors.lastname: Okabayashi
authors.forename: Pratap
authors.initials: P
authors.lastname: Singhasivanon
authors.forename: Natthanej
authors.initials: N
authors.lastname: Luplertlop
mesh_terms: D065632:Chikungunya Fever / Q000453:epidemiology* / Q000821:virology*; D002646:Chikungunya virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D014644:Genetic Variation; D006801:Humans; D008958:Models, Molecular; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D044967:Serum / Q000821:virology; D013785:Thailand / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014759:Viral Envelope Proteins
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