title: Tts1, the fission yeast homologue of the TMEM33 family, functions in NE remodeling during mitosis.
abstract: The fission yeast Schizosaccharomyces pombe undergoes "closed" mitosis in which the nuclear envelope (NE) stays intact throughout chromosome segregation. Here we show that Tts1, the fission yeast TMEM33 protein that was previously implicated in organizing the peripheral endoplasmic reticulum (ER), also functions in remodeling the NE during mitosis. Tts1 promotes insertion of spindle pole bodies (SPBs) in the NE at the onset of mitosis and modulates distribution of the nuclear pore complexes (NPCs) during mitotic NE expansion. Structural features that drive partitioning of Tts1 to the high-curvature ER domains are crucial for both aspects of its function. An amphipathic helix located at the C-terminus of Tts1 is important for ER shaping and modulating the mitotic NPC distribution. Of interest, the evolutionarily conserved residues at the luminal interface of the third transmembrane region function specifically in promoting SPB-NE insertion. Our data illuminate cellular requirements for remodeling the NE during "closed" nuclear division and provide insight into the structure and functions of the eukaryotic TMEM33 family.
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604 zhangdan@tll.org.sg snezhana.oliferenko@kcl.ac.uk.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604 Department of Biological Sciences, National University of Singapore, Singapore 117604 zhangdan@tll.org.sg snezhana.oliferenko@kcl.ac.uk.
authors.forename: Snezhana
authors.initials: S
authors.lastname: Oliferenko
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D020090:Chromosome Segregation / Q000235:genetics*; D004721:Endoplasmic Reticulum / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics; D008938:Mitosis / Q000235:genetics; D009685:Nuclear Envelope / Q000502:physiology*; D022022:Nuclear Pore / Q000235:genetics; D028861:Nuclear Pore Complex Proteins / Q000235:genetics; D017434:Protein Structure, Tertiary; D012568:Schizosaccharomyces / Q000235:genetics / Q000254:growth & development*; D029702:Schizosaccharomyces pombe Proteins; D017422:Sequence Analysis, DNA; D064048:Spindle Pole Bodies / Q000235:genetics* / Q000378:metabolism; D013329:Structure-Activity Relationship; D059470:Tetraspanins / Q000235:genetics / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins; D028861:Nuclear Pore Complex Proteins; D029702:Schizosaccharomyces pombe Proteins; D059470:Tetraspanins; C000598470:Tts1 protein, S pombe
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