title: Rapid clearance of epigenetic protein reporters from wound edge cells inDrosophilalarvae does not depend on the JNK or PDGFR/VEGFR signaling pathways.
abstract: The drastic cellular changes required for epidermal cells to dedifferentiate and become motile during wound closure are accompanied by changes in gene transcription, suggesting corresponding alterations in chromatin. However, the epigenetic changes that underlie wound-induced transcriptional programs remain poorly understood partly because a comprehensive study of epigenetic factor expression during wound healing has not been practical. To determine which chromatin modifying factors might contribute to wound healing, we screened publicly available fluorescently-tagged reporter lines inDrosophilafor altered expression at the wound periphery during healing. Thirteen reporters tagging seven different proteins showed strongly diminished expression at the wound edge. Three downregulated proteins, Osa, Kismet, and Spt6, are generally associated with active chromatin, while four others, Sin3A, Sap130, Mi-2, and Mip120, are associated with repressed chromatin. In all cases reporter down regulation was independent of the Jun N-terminal Kinase and Pvr pathways, suggesting that novel signals control reporter clearance. Taken together, our results suggest that clearance of chromatin modifying factors may enable wound edge cells to rapidly and comprehensively change their transcriptional state following tissue damage.
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, Unit 1000, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
authors.forename: Aimee E
authors.initials: AE
authors.lastname: Anderson
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, Unit 1000, 1515 Holcombe Boulevard, Houston, TX 77030, USA ; Genes & Development Graduate Program, The University of Texas MD Anderson Cancer Center, Unit 1000, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Galko
keywords: Drosophila; Polycomb; epigenetic; trithorax; wound healing
references.citation: J Am Soc Nephrol. 2008 Jul;19(7):1311-20
references.pmid: 18322163
references.citation: PLoS Genet. 2012;8(8):e1002878
references.pmid: 22912596
references.citation: Proc Natl Acad Sci U S A. 1988 Nov;85(21):8136-40
references.pmid: 3141923
references.citation: Nature. 2002 Dec 19-26;420(6917):833-7
references.pmid: 12490953
references.citation: J Cell Sci. 2012 Dec 1;125(Pt 23):5667-76
references.pmid: 22976306
references.citation: Semin Cell Dev Biol. 2009 Jul;20(5):565-74
references.pmid: 19146968
references.citation: Curr Biol. 2012 Jan 10;22(1):R14-6
references.pmid: 22240471
references.citation: Cell. 2007 Feb 23;128(4):693-705
references.pmid: 17320507
references.citation: J Cell Physiol. 2006 Apr;207(1):1-11
references.pmid: 16155926
references.citation: Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2224-9
references.pmid: 19168633
references.citation: Trends Cell Biol. 2009 Jun;19(6):286-94
references.pmid: 19394225
references.citation: Cell Stem Cell. 2010 Oct 8;7(4):455-69
references.pmid: 20887952
references.citation: Cell. 2006 Dec 15;127(6):1209-21
references.pmid: 17174895
references.citation: Science. 2005 Apr 15;308(5720):381-5
references.pmid: 15831751
references.citation: Genes Dev. 2000 Oct 15;14(20):2623-34
references.pmid: 11040216
references.citation: Nature. 2009 Jun 18;459(7249):996-9
references.pmid: 19494811
references.citation: PLoS One. 2013 Apr 24;8(4):e61773
references.pmid: 23637905
references.citation: Mol Biol Cell. 2012 Mar;23(6):1068-79
references.pmid: 22262460
references.citation: Genetics. 1999 Sep;153(1):135-77
references.pmid: 10471706
references.citation: Nat Methods. 2011 Sep;8(9):737-43
references.pmid: 21985007
references.citation: Genetics. 2010 Nov;186(3):943-57
references.pmid: 20813879
references.citation: Development. 2005 Apr;132(7):1623-35
references.pmid: 15728673
references.citation: J Surg Res. 2009 May 15;153(2):347-58
references.pmid: 19027922
references.citation: J Cell Sci. 2009 Sep 15;122(Pt 18):3209-13
references.pmid: 19726630
references.citation: J Biol Chem. 2003 Jul 4;278(27):24624-8
references.pmid: 12730213
references.citation: Curr Biol. 2009 Sep 15;19(17):1473-7
references.pmid: 19646875
references.citation: Proc Natl Acad Sci U S A. 1987 Nov;84(21):7696-700
references.pmid: 3499612
references.citation: Genetics. 2010 Jan;184(1):129-40
references.pmid: 19884309
references.citation: EMBO Rep. 2009 Aug;10(8):881-6
references.pmid: 19575012
references.citation: Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):19889-94
references.pmid: 19897725
references.citation: Nat Cell Biol. 2009 Jul;11(7):890-5
references.pmid: 19525935
references.citation: Worm. 2012 Apr 1;1(2):134-8
references.pmid: 24058838
references.citation: Development. 1999 Feb;126(4):733-42
references.pmid: 9895321
references.citation: Curr Biol. 2010 Mar 9;20(5):464-70
references.pmid: 20188558
references.citation: Curr Biol. 2013 Mar 4;23(5):424-9
references.pmid: 23394834
references.citation: Genetics. 2011 Jul;188(3):731-43
references.pmid: 21515576
references.citation: PLoS Genet. 2008 Oct;4(10):e1000217
references.pmid: 18846226
references.citation: Genetics. 2007 Mar;175(3):1089-104
references.pmid: 17179094
references.citation: Methods Mol Biol. 2013;1037:449-61
references.pmid: 24029952
references.citation: Nucleic Acids Res. 2004 Jan 1;32(Database issue):D418-20
references.pmid: 14681446
references.citation: Dev Biol. 2002 Jan 1;241(1):145-56
references.pmid: 11784101
references.citation: EMBO Rep. 2011 Mar;12(3):208-15
references.pmid: 21311559
references.citation: Nat Cell Biol. 2013 Sep;15(9):1123-30
references.pmid: 23934216
references.citation: Genes Dev. 1997 Aug 1;11(15):1949-62
references.pmid: 9271118
references.citation: Anat Rec (Hoboken). 2012 Oct;295(10):1541-51
references.pmid: 22933452
references.citation: Science. 1998 Dec 4;282(5395):1897-900
references.pmid: 9836641
references.citation: Cell. 2007 Feb 23;128(4):635-8
references.pmid: 17320500
references.citation: J Assoc Res Otolaryngol. 2009 Sep;10(3):341-53
references.pmid: 19340485
references.citation: Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15050-5
references.pmid: 11742088
references.citation: Gene. 2002 Jun 26;293(1-2):107-14
references.pmid: 12137948
references.citation: PLoS Genet. 2011 Dec;7(12):e1002424
references.pmid: 22242003
references.citation: Methods Enzymol. 2013;528:237-55
references.pmid: 23849869
references.citation: Dev Genes Evol. 1998 Nov;208(9):531-6
references.pmid: 9799435
references.citation: Genes Dev. 2004 Dec 1;18(23):2929-40
references.pmid: 15545624
references.citation: Curr Biol. 2011 Dec 6;21(23):1960-7
references.pmid: 22100061
references.citation: Dis Model Mech. 2011 Sep;4(5):569-74
references.pmid: 21810906
references.citation: FEBS Lett. 2012 Mar 23;586(6):772-7
references.pmid: 22449966
references.citation: J Cell Biol. 2012 Oct 15;199(2):225-34
references.pmid: 23045550
references.citation: Dev Biol. 2005 Apr 1;280(1):73-86
references.pmid: 15766749
references.citation: Curr Opin Cell Biol. 2008 Jun;20(3):266-73
references.pmid: 18439810
references.citation: Mech Dev. 2002 Jan;110(1-2):71-83
references.pmid: 11744370
references.citation: Dev Cell. 2003 Jun;4(6):865-77
references.pmid: 12791271
references.citation: EMBO J. 2000 Aug 15;19(16):4332-41
references.pmid: 10944116
references.citation: Nature. 2010 Mar 25;464(7288):606-9
references.pmid: 20336145
references.citation: Mech Dev. 2012 Sep-Dec;129(9-12):208-18
references.pmid: 22947425
references.citation: PLoS Biol. 2004 Aug;2(8):E239
references.pmid: 15269788
references.citation: Bioinformatics. 2009 Feb 15;25(4):548-9
references.pmid: 19126575
references.citation: Genetics. 2007 Mar;175(3):1505-31
references.pmid: 17194782
references.citation: J Biol Chem. 2010 Aug 27;285(35):27457-27467
references.pmid: 20566628
references.citation: Nat Rev Mol Cell Biol. 2013 Apr;14(4):249-62
references.pmid: 23443750
references.citation: FEBS Lett. 2011 Jun 6;585(11):1617-24
references.pmid: 21569771