title: Chemotherapy and remission status do not alter pre-existing innate immune dysfunction in dogs with lymphoma.
abstract: Dogs with lymphoma have altered innate immunity and little is known about the effects of chemotherapy on innate immune function in dogs. Lipopolysaccharide (LPS), lipoteichoic acid (LTA), and peptidoglycan (PG) - induced leukocyte cytokine production capacity, and phagocytosis and respiratory burst were evaluated in dogs prior to and following 6 weeks of chemotherapy. Dogs had decreased TNF production following LPS stimulation and increased IL-10 production following PG stimulation, which did not improve following remission of lymphoma. Dogs also had reduced E. coli-induced respiratory burst function after chemotherapy induced complete or partial remission. Dogs with lymphoma have an imbalance in pro-and anti-inflammatory cytokine production which did not improve with remission, and, following treatment, a decrease in respiratory burst function. Altered immune responses following exposure to bacterial pathogen associated molecular pattern motifs and bacteria may have many implications in the management of canine lymphoma.
authors.affiliation: Comparative Oncology and Epigenetics Laboratory, Department of Veterinary Medicine and Surgery, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.
authors.forename: S
authors.initials: S
authors.lastname: Axiak-Bechtel
authors.affiliation: Comparative Internal Medicine Laboratory, Department of Veterinary Medicine and Surgery, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.
authors.forename: B
authors.initials: B
authors.lastname: Fowler
authors.affiliation: College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, 17 Gwangju, Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Yu
authors.affiliation: Comparative Internal Medicine Laboratory, Department of Veterinary Medicine and Surgery, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.
authors.forename: J
authors.initials: J
authors.lastname: Amorim
authors.affiliation: Comparative Internal Medicine Laboratory, Department of Veterinary Medicine and Surgery, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.
authors.forename: K
authors.initials: K
authors.lastname: Tsuruta
authors.affiliation: Comparative Internal Medicine Laboratory, Department of Veterinary Medicine and Surgery, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA. Electronic address: decluea@missouri.edu.
authors.forename: A
authors.initials: A
authors.lastname: DeClue
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016207:Cytokines / Q000378:metabolism; D004283:Dog Diseases / Q000188:drug therapy* / Q000276:immunology*; D004285:Dogs; D004358:Drug Therapy; D004926:Escherichia coli / Q000502:physiology; D005500:Follow-Up Studies; D007113:Immunity, Innate / Q000187:drug effects* / Q000276:immunology; D016753:Interleukin-10 / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008223:Lymphoma / Q000188:drug therapy* / Q000276:immunology / Q000662:veterinary*; D010457:Peptidoglycan / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D012074:Remission Induction; D016897:Respiratory Burst / Q000187:drug effects / Q000502:physiology; D013682:Teichoic Acids / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D013682:Teichoic Acids; D016753:Interleukin-10; C009900:lipoteichoic acid
keywords: Chemotherapy; Innate immunity; Lymphoma; Neutrophils