title: Lutein inhibits the migration of retinal pigment epithelial cells via cytosolic and mitochondrial Akt pathways (lutein inhibits RPE cells migration).
abstract: During the course of proliferative vitreoretinopathy (PVR), the retinal pigment epithelium (RPE) cells will de-differentiate, proliferate, and migrate onto the surfaces of the sensory retina. Several studies have shown that platelet-derived growth factor (PDGF) can induce migration of RPE cells via an Akt-related pathway. In this study, the effect of lutein on PDGF-BB-induced RPE cells migration was examined using transwell migration assays and Western blot analyses. We found that both phosphorylation of Akt and mitochondrial translocation of Akt in RPE cells induced by PDGF-BB stimulation were suppressed by lutein. Furthermore, the increased migration observed in RPE cells with overexpressed mitochondrial Akt could also be suppressed by lutein. Our results demonstrate that lutein can inhibit PDGF-BB induced RPE cells migration through the inhibition of both cytoplasmic and mitochondrial Akt activation.
authors.affiliation: Graduate Institute of Applied Science and Engineering, Fu Jen Catholic University,New Taipei City 24205, Taiwan. m212092001@tmu.edu.tw.
authors.forename: Ching-Chieh
authors.initials: CC
authors.lastname: Su
authors.affiliation: School of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan. m212092001@tmu.edu.tw.
authors.forename: Chi-Ming
authors.initials: CM
authors.lastname: Chan
authors.affiliation: Graduate Institute of Applied Science and Engineering, Fu Jen Catholic University,New Taipei City 24205, Taiwan. 056489@mail.fju.edu.tw.
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Chen
authors.affiliation: Department of Life Sciences, Fu Jen Catholic University, New Taipei City 24205, Taiwan. bobwu80113@gmail.com.
authors.forename: Chia-Chun
authors.initials: CC
authors.lastname: Wu
authors.affiliation: Department of Nutrition and Health Science, Chang Guang University of Science and Technology,Taoyuan 33303, Taiwan. mozart@gw.cgust.edu.tw.
authors.forename: Chien-Yu
authors.initials: CY
authors.lastname: Hsiao
authors.affiliation: Slone Epidemiology Center, Boston University, Boston, Massachusetts, United States of America,Boston, MA 02215, USA. peilan@bu.edu.
authors.forename: Pei-Lan
authors.initials: PL
authors.lastname: Lee
authors.affiliation: Graduate Institute of Applied Science and Engineering, Fu Jen Catholic University,New Taipei City 24205, Taiwan. suk.ccsu@gmail.com.
authors.forename: Victor Chia-Hsiang
authors.initials: VC
authors.lastname: Lin
authors.affiliation: School of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan. 054317@mail.fju.edu.tw.
authors.forename: Chi-Feng
authors.initials: CF
authors.lastname: Hung
mesh_terms: D000077214:Becaplermin; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D003600:Cytosol / Q000378:metabolism*; D006801:Humans; D014975:Lutein / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D051057:Proto-Oncogene Proteins c-akt; D014975:Lutein
references.citation: Am J Physiol Heart Circ Physiol. 2006 Jun;290(6):H2402-8
references.pmid: 16687609
references.citation: Ann N Y Acad Sci. 1992 Sep 30;669:7-20
references.pmid: 1444060
references.citation: Circ Res. 2004 Apr 16;94(7):884-91
references.pmid: 14988230
references.citation: J Biol Chem. 2003 Aug 15;278(33):30945-51
references.pmid: 12773546
references.citation: Mol Cell Biol. 2011 May;31(9):1788-99
references.pmid: 21357737
references.citation: Am J Physiol Heart Circ Physiol. 2012 Feb 1;302(3):H716-23
references.pmid: 22081709
references.citation: Invest Ophthalmol Vis Sci. 1986 Nov;27(11):1644-52
references.pmid: 2429937
references.citation: Invest Ophthalmol Vis Sci. 2002 Feb;43(2):483-9
references.pmid: 11818394
references.citation: Nippon Ganka Gakkai Zasshi. 1990 Apr;94(4):340-51
references.pmid: 1697726
references.citation: Recent Pat Biotechnol. 2008;2(3):218-26
references.pmid: 19097333
references.citation: Free Radic Biol Med. 2008 Sep 15;45(6):885-96
references.pmid: 18620044
references.citation: J Biomed Biotechnol. 2010;2010:190724
references.pmid: 20182540
references.citation: Am J Ophthalmol. 1985 Jul 15;100(1):188-93
references.pmid: 4014372
references.citation: Surv Ophthalmol. 1992 Mar-Apr;36(5):373-84
references.pmid: 1566240
references.citation: Invest Ophthalmol Vis Sci. 1992 Sep;33(10):2830-8
references.pmid: 1526732
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11946-51
references.pmid: 16882732
references.citation: J Mol Cell Cardiol. 2009 Jun;46(6):919-26
references.pmid: 19249309
references.citation: Nutr Cancer. 2011;63(1):39-45
references.pmid: 21128180
references.citation: Mol Cell Endocrinol. 2003 Jul 31;205(1-2):99-106
references.pmid: 12890571
references.citation: Biochem Biophys Res Commun. 2009 Oct 9;388(1):172-6
references.pmid: 19664603
references.citation: Int J Ophthalmol. 2011;4(3):303-6
references.pmid: 22553667
references.citation: J Neurochem. 2003 Dec;87(6):1427-35
references.pmid: 14713298
references.citation: J Photochem Photobiol B. 2013 Jul 5;124:34-41
references.pmid: 23651647
references.citation: Molecules. 2011 Feb 18;16(2):1710-38
references.pmid: 21336241
references.citation: Invest Ophthalmol Vis Sci. 1999 Oct;40(11):2683-9
references.pmid: 10509666
references.citation: Jpn J Ophthalmol. 2003 Mar-Apr;47(2):158-65
references.pmid: 12738549
references.citation: Exp Eye Res. 1997 Sep;65(3):365-78
references.pmid: 9299173
references.citation: PLoS One. 2013;8(2):e56819
references.pmid: 23457620
references.citation: Invest Ophthalmol Vis Sci. 2002 Jul;43(7):2406-11
references.pmid: 12091444
references.citation: Biochem Biophys Res Commun. 2006 Jun 9;344(3):912-9
references.pmid: 16631611
references.citation: Annu Rev Nutr. 2003;23:171-201
references.pmid: 12626691
references.citation: J Cell Biochem. 2007 Sep 1;102(1):196-210
references.pmid: 17340627
references.citation: J Nutr Biochem. 2006 Nov;17(11):780-6
references.pmid: 16563717
references.citation: Invest Ophthalmol Vis Sci. 2005 Oct;46(10):3562-9
references.pmid: 16186334
references.citation: Nat Rev Mol Cell Biol. 2001 May;2(5):339-49
references.pmid: 11331908
references.citation: Kaohsiung J Med Sci. 2014 Jan;30(1):1-11
references.pmid: 24388052
references.citation: Surv Ophthalmol. 1998 Jul-Aug;43(1):3-18
references.pmid: 9716190
references.citation: Invest Ophthalmol Vis Sci. 1994 Sep;35(10):3649-63
references.pmid: 8088954
references.citation: J Mol Cell Cardiol. 2013 Jun;59:167-75
references.pmid: 23500391
references.citation: Cutan Ocul Toxicol. 2014 Jun;33(2):132-7
references.pmid: 23862688
references.citation: Mol Vis. 2010 Apr 03;16:586-95
references.pmid: 20376327
references.citation: Arch Ophthalmol. 1997 Feb;115(2):237-41
references.pmid: 9046259
references.citation: J Biomed Sci. 2012 Feb 08;19:18
references.pmid: 22313606