title: Immune-enhancing activity of polysaccharides from Cyrtomium macrophyllum.
abstract: The goal of the present study was to investigate the immune-enhancing activity of polysaccharides from Cyrtomium macrophyllum (CMP). Two experiments were carried out. In immunosuppression experiment, the immune-enhancing effect of CMP in immunosuppressive mice was performed. The results showed that CMP at high and medium doses was able to overcome the CY-induced immunosuppression, significantly increases the thymus and spleen indices, enhances lymphocyte proliferation activity and macrophage function, improves immunoglobulin and cytokines levels compared with negative control group. In macrophage immunomodulatory experiment, the immune-enhancing effect of CMP in RAW264.7 cells was measured. The results showed that CMP induced the elevation of NO production, TNF-α secretion and iNOS protein of RAW264.7 cells. CMP can also strongly increase NF-κB levels in nuclear, which is an important transcription factor that can modulate expressions of iNOS, NO and TNF-α. Therefore, the CMP could be an effective immunomodulatory agent.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ren
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Chenghua
authors.initials: C
authors.lastname: He
authors.affiliation: Wujiang Agricultural Commission, Wujiang 215200, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Fan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Si
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Shi
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400, China.
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: haibinzhang12@163.com.
authors.forename: Haibin
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D029624:Ferns / Q000737:chemistry*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D010641:Phenotype; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D016207:Cytokines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007155:Immunologic Factors; D016328:NF-kappa B; D010936:Plant Extracts; D011134:Polysaccharides; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II
keywords: Cyclophosphamide; Cyrtomium macrophyllum polysaccharide; Immune-enhancing activity