title: Magnetic properties and antitumor effect of nanocomplexes of iron oxide and doxorubicin.
abstract: We present a technology and magneto-mechanical milling chamber for the magneto-mechano-chemical synthesis (MMCS) of magneto-sensitive complex nanoparticles (MNC) comprising nanoparticles Fe3O4 and anticancer drug doxorubicin (DOXO). Magnetic properties of MNC were studied with vibrating magnetometer and electron paramagnetic resonance. Under the influence of mechano-chemical and MMCS, the complex show a hysteresis curve, which is typical for soft ferromagnetic materials. We also demonstrate that Lewis lung carcinoma had a hysteresis loop typical for a weak soft ferromagnet in contrast to surrounding tissues, which were diamagnetic. Combined action of constant magnetic field and radio frequency moderate inductive hyperthermia (RFH) below 40°C and MNC was found to induce greater antitumor and antimetastatic effects as compared to conventional DOXO. Radiospectroscopy shows minimal activity of FeS-protein electron transport chain of mitochondria, and an increase in the content of non-heme iron complexes with nitric oxide in the tumor tissues under the influence of RFH and MNC.
authors.affiliation: National Cancer Institute, Kyiv, Ukraine. Electronic address: v-orel@voliacable.com.
authors.forename: Valerii
authors.initials: V
authors.lastname: Orel
authors.affiliation: G.V. Kurdyumov Institute for Metal Physics, 36 Academican Vernadsky Blvd., Kyiv, Ukraine.
authors.forename: Anatoliy
authors.initials: A
authors.lastname: Shevchenko
authors.affiliation: National Cancer Institute, Kyiv, Ukraine.
authors.forename: Andriy
authors.initials: A
authors.lastname: Romanov
authors.affiliation: Cavendish Laboratory, University of Cambridge, J.J. Thomson Avenue, Cambridge, United Kingdom; Cavendish NanoTherapeutics Ltd, CPC1 Capital Park, Fulbourn, Cambridge, United Kingdom.
authors.forename: Marina
authors.initials: M
authors.lastname: Tselepi
authors.affiliation: Cavendish Laboratory, University of Cambridge, J.J. Thomson Avenue, Cambridge, United Kingdom; Cavendish NanoTherapeutics Ltd, CPC1 Capital Park, Fulbourn, Cambridge, United Kingdom.
authors.forename: Thanos
authors.initials: T
authors.lastname: Mitrelias
authors.affiliation: Cavendish Laboratory, University of Cambridge, J.J. Thomson Avenue, Cambridge, United Kingdom.
authors.forename: Crispin H W
authors.initials: CH
authors.lastname: Barnes
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, Ukraine.
authors.forename: Anatoliy
authors.initials: A
authors.lastname: Burlaka
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, Ukraine.
authors.forename: Sergey
authors.initials: S
authors.lastname: Lukin
authors.affiliation: National Cancer Institute, Kyiv, Ukraine.
authors.forename: Igor
authors.initials: I
authors.lastname: Shchepotin
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D018827:Carcinoma, Lewis Lung; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005290:Ferric Compounds / Q000737:chemistry; D005609:Free Radicals; D006979:Hyperthermia, Induced; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*; D036103:Nanotechnology / Q000379:methods*; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D005290:Ferric Compounds; D005609:Free Radicals; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D004317:Doxorubicin
keywords: Doxorubicin; Free radicals; Lewis lung carcinoma; Magnetic nanoparticles; Magneto-mechano-chemical synthesis