title: Maternal mRNA expression levels of H19 are inversely associated with risk of macrosomia.
abstract: BACKGROUND
  To investigate the associations between the mRNA levels of H19 in term placenta and risk of macrosomia.
  
  
  METHODS
  Term placentas were collected from 37 macrosomia and 37 matched neonates with normal birth weight (controls) born in Changzhou Women and Children Health Hospital, Jiangsu province, P. R. China from March 1 to June 30, 2008. The mRNA levels of H19 in those placentas were measured by real-time polymerase chain reaction (PCR). Simple and multiple logistic regression models were used to explore the risk factors in the development of macrosomia. All analyses were performed using Stata 10.0 (StataCorp, College Station, Texas, USA).
  
  
  RESULTS
  The average H19 mRNA level of the macrosomia group was 1.450 ±0.456 while in the control group it was 2.080 ±1.296. Based on the result of Student's t test, there was a significant difference in H19 mRNA level between the macrosomia group and the control group (p = 0.008). After controlling for potential confounders, the multivariable adjusted odds ratio (OR) of macrosomia for those in the highest tertile of H19 mRNA level was 0.12 (95% CI: 0.02-0.59) when compared to those in the lowest tertile (p for linear trend = 0.009).
  
  
  CONCLUSIONS
  The term placental H19 mRNA levels were inversely related to the occurrence of macrosomia. Our findings suggest that the low expression of H19 mRNA may contribute to the development of macrosomia.
authors.affiliation: Changzhou Maternity and Child Health Hospital Affiliated to Nanjing Medical University Jiangsu, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Comprehensive Laboratory, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Nutrition, Gillings Schools of Global Public Health and School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, USA ; Department of Epidemiology, Gillings Schools of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, USA.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Xun
authors.affiliation: Comprehensive Laboratory, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Comprehensive Laboratory, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Luo
authors.affiliation: Changzhou Maternity and Child Health Hospital Affiliated to Nanjing Medical University Jiangsu, China.
authors.forename: Qiuwei
authors.initials: Q
authors.lastname: Wang
keywords: H19; insulin-like growth factor II; macrosomia; placenta; real-time polymerase chain reaction
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