title: Finding and mapping new genes faster than ever: revisited.
abstract: This article recounts some of the early days of the Human Genome Project, covering the important and sometimes controversial role that complementary DNA-based approaches played in the discovery and mapping of the majority of human genes. It also describes my involvement in this effort and my lab's development of methods for rapid sequence identification and mapping of human genes.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, Colorado 80045-0508 james.sikela@ucdenver.edu.
authors.forename: James M
authors.initials: JM
authors.lastname: Sikela
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015894:Genome, Human; D049673:History, 20th Century; D016045:Human Genome Project / Q000266:history*; D006801:Humans; D053766:Presenilin-2 / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D020413:3' Untranslated Regions; D018076:DNA, Complementary; D053766:Presenilin-2
references.citation: Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9228-32
references.pmid: 7937745
references.citation: Nat Genet. 1992 Nov;2(3):180-5
references.pmid: 1345165
references.citation: Nucleic Acids Res. 1991 Apr 11;19(7):1715
references.pmid: 2027783
references.citation: Nucleic Acids Res. 1991 Apr 25;19(8):1837-43
references.pmid: 2030965
references.citation: Science. 1991 Jun 21;252(5013):1651-6
references.pmid: 2047873
references.citation: Nat Genet. 1995 Aug;10(4):415-23
references.pmid: 7670491
references.citation: Science. 1989 Sep 29;245(4925):1434-5
references.pmid: 2781285
references.citation: Nature. 1995 Jun 29;375(6534):754-60
references.pmid: 7596406
references.citation: Nat Genet. 1993 Mar;3(3):189-91
references.pmid: 8485571
references.citation: Science. 1998 Oct 23;282(5389):744-6
references.pmid: 9784132
references.citation: Nat Genet. 1995 Aug;10(4):369-71
references.pmid: 7670480
references.citation: Science. 1996 Oct 25;274(5287):540-6
references.pmid: 8849440
references.citation: FASEB J. 1991 Jan;5(1):35-9
references.pmid: 1991581
references.citation: Science. 1995 Aug 18;269(5226):973-7
references.pmid: 7638622