title: Morphological and molecular characterization of the two known North American Chlorociboria species and their anamorphs.
abstract: This study confirms that the two known Chlorociboria species from North America correspond to Chlorociboria aeruginascens and Chlorociboria aeruginosa that were originally described from Europe. The anamorphs of these two species are unambiguously identified for the first time: the genetic connection between C. aeruginascens is Dothiorina tulasnei, is here demonstrated for the first time by molecular data, and the anamorph of C. aeruginosa was previously undescribed. These two species are more closely related to different Southern Hemisphere taxa than they are to each other, indicating complex speciation processes in a global geographic context. Pure cultures isolated from the two species were grown on various media for examination of growth rate, sporulation, and xylindein production. The latter is responsible for green staining of wood and has applications in craftsmanship and perhaps also for drug development.
authors.affiliation: Faculty of Forestry, University of Toronto, 33 Willcocks Street, Toronto, ON M5S 3B3, Canada. Electronic address: daniela.tudor@utoronto.ca.
authors.forename: Daniela
authors.initials: D
authors.lastname: Tudor
authors.affiliation: Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, ON M5S 2C6, Canada.
authors.forename: Simona
authors.initials: S
authors.lastname: Margaritescu
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Toronto, 100 Queen's Park, Toronto, ON M5S 2C6, Canada.
authors.forename: Santiago
authors.initials: S
authors.lastname: Sánchez-Ramírez
authors.affiliation: Faculty of Forestry, University of Toronto, 33 Willcocks Street, Toronto, ON M5S 3B3, Canada. Electronic address: sara.robinson@oregonstate.edu.
authors.forename: Sara C
authors.initials: SC
authors.lastname: Robinson
authors.affiliation: Faculty of Forestry, University of Toronto, 33 Willcocks Street, Toronto, ON M5S 3B3, Canada. Electronic address: p.cooper@utoronto.ca.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Cooper
authors.affiliation: Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, ON M5S 2C6, Canada; Department of Ecology and Evolutionary Biology, University of Toronto, 100 Queen's Park, Toronto, ON M5S 2C6, Canada.
authors.forename: Jean Marc
authors.initials: JM
authors.lastname: Moncalvo
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000166:cytology* / Q000254:growth & development / Q000302:isolation & purification; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D008853:Microscopy; D008969:Molecular Sequence Data; D009656:North America; D010636:Phenols / Q000378:metabolism; D010802:Phylogeny; D011083:Polycyclic Compounds / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000166:cytology / Q000254:growth & development
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D010636:Phenols; D011083:Polycyclic Compounds; C419334:xylindein
keywords: Dothiorina tulasnei; ITS; Phylogeny; Wood staining; Xylindein