title: Purification and characterization of a novel alkaline β-1,3-1,4-glucanase (lichenase) from thermophilic fungus Malbranchea cinnamomea.
abstract: A novel alkaline β-1,3-1,4-glucanase (McLic1) from a thermophilic fungus, Malbranchea cinnamomea, was purified and biochemically characterized. McLic1 was purified to homogeneity with a purification fold of 3.1 and a recovery yield of 3.7 %. The purified enzyme was most active at pH 10.0 and 55 °C, and exhibited a wide range of pH stability (pH 4.0-10.0). McLic1 displayed strict substrate specificity for barley β-glucan, oat β-glucan and lichenan, but did not show activity towards other tested polysaccharides and synthetic p-nitrophenyl derivates, suggesting that it is a specific β-1,3-1,4-glucanase. The K m values for barley β-glucan, oat β-glucan and lichenan were determined to be 0.69, 1.11 and 0.63 mg mL(-1), respectively. Moreover, the enzyme was stable in various non ionic surfactants, oxidizing agents and several commercial detergents. Thus, the alkaline β-1,3-1,4-glucanase may have potential in industrial applications, such as detergent, paper and pulp industries.
authors.affiliation: Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, PO Box 294, Beijing, 100083, China.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Yang
authors.forename: Hao
authors.initials: H
authors.lastname: Xiong
authors.forename: Qiaojuan
authors.initials: Q
authors.lastname: Yan
authors.forename: Hongye
authors.initials: H
authors.lastname: Yang
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology*; D003902:Detergents / Q000737:chemistry; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005936:Glucans / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D047071:beta-Glucans / Q000737:chemistry
chemical_list: D003902:Detergents; D005656:Fungal Proteins; D005936:Glucans; D047071:beta-Glucans; C067858:beta-glucan, (1-3)(1-4)-; D006026:Glycoside Hydrolases; C028020:licheninase; C015968:lichenin
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