title: Measuring transport of motor cargos.
abstract: In this chapter, we describe experimental techniques used in vitro to illuminate how small teams of motors can work to translocate cargos. We will focus on experiments utilizing in vitro reconstitution, artificial or ex vivo purified cargos, and fluorescence imaging. A number of studies have been able to recapitulate the activities of cargo transport driven by small teams of motors elucidating how multiple motors can work together to transport cargos within the cell. Here, we describe some of the methods employed and highlight important experimental details needed to perform these experiments.
authors.affiliation: Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, Amherst, MA, USA.
authors.forename: Leslie
authors.initials: L
authors.lastname: Conway
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Ross
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D020409:Molecular Motor Proteins / Q000378:metabolism*; D015336:Molecular Probe Techniques; D061848:Optical Imaging / Q000379:methods*; D021381:Protein Transport
chemical_list: D005456:Fluorescent Dyes; D020409:Molecular Motor Proteins