title: Mincle signaling in the innate immune response after traumatic brain injury.
abstract: The innate immune response contributes to the inflammatory activity after traumatic brain injury (TBI). In the present study we identify macrophage-inducible C-type lectin (mincle) as a pattern recognition receptor that contributes to innate immunity in neurons after TBI. Here we report that mincle is activated by SAP130 in cortical neurons in culture, resulting in production of the inflammatory cytokine TNF. In addition, mincle and SAP130 are elevated in the brain and cerebrospinal fluid of humans after TBI and the brain of rodents after fluid percussion brain injury. Thus, these findings suggest the involvement of mincle to the pathology of TBI. Importantly, blocking mincle with a neutralizing antibody against mincle in cortical neurons in culture treated with SAP130 resulted in inhibition of mincle signaling and decreased TNF production. Therefore, our findings identify mincle as a contributor to the inflammatory response after TBI.
authors.affiliation: 1 Ophthalmology Department, Louisiana State University School of Medicine/Ochsner Medical Center , New Orleans, Louisiana.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: de Rivero Vaccari
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Brand
authors.forename: Aldo F
authors.initials: AF
authors.lastname: Berti
authors.forename: Ofelia F
authors.initials: OF
authors.lastname: Alonso
authors.forename: M Ross
authors.initials: MR
authors.lastname: Bullock
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: de Rivero Vaccari
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001930:Brain Injuries / Q000276:immunology*; D005260:Female; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000276:immunology*; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D051381:Rats; D017207:Rats, Sprague-Dawley; D011971:Receptors, Immunologic / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D055815:Young Adult
chemical_list: C482593:CLEC4D protein, human; D037181:Lectins, C-Type; D011971:Receptors, Immunologic
keywords: innate immunity; mincle; neuroinflammation; neurons; traumatic brain injury