title: Crystal and solution structure of the human RIG-I SF2 domain.
abstract: RIG-I is a pathogen-recognition receptor that recognizes viral 5'-triphosphates carrying double-stranded RNA. Upon binding to these microbe-associated molecular patterns (MAMPs), RIG-I forms oligomers and promotes downstream processes that result in type I interferon production and induction of an antiviral state. Here, the crystal structure of the human RIG-I superfamily 2 ATPase domain crystallized in an unusually elongated and open conformation is reported. The elongated structure is probably induced in part by crystal packing, but nevertheless indicates that the domain is intrinsically very flexible. This flexibility might allow substantial structural changes upon substrate binding and oligomerization.
authors.affiliation: Gene Center and Department of Biochemistry, Ludwig-Maximilians University Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Deimling
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, 9 Dong Dan San Tiao, Beijing 100730, People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Cui
authors.affiliation: Gene Center and Department of Biochemistry, Ludwig-Maximilians University Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Lammens
authors.affiliation: Gene Center and Department of Biochemistry, Ludwig-Maximilians University Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors.forename: Karl-Peter
authors.initials: KP
authors.lastname: Hopfner
authors.affiliation: Gene Center and Department of Biochemistry, Ludwig-Maximilians University Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors.forename: Gregor
authors.initials: G
authors.lastname: Witte
mesh_terms: D002850:Chromatography, Gel; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000737:chemistry*; D006801:Humans; D008027:Light; D011487:Protein Conformation; D011971:Receptors, Immunologic; D012542:Scattering, Radiation
chemical_list: D011971:Receptors, Immunologic; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases
keywords: RIG-I; SF2 helicase
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