title: Effects of activated protein C on the size of modeled ischemic focus and morphometric parameters of neurons and neuroglia in its perifocal zone.
abstract: The effects of activated protein C (APC) on the quantitative parameters of neurons and neuroglia in the perifocal zone of infarction induced in the left hemispheric cortex were studied in two groups of rats. Group 1 animals served as control (control infarction). Group 2 rats were injected with APC (50 μg/kg) in the right lateral cerebral ventricle 3 h after infarction was induced, and after 72 h the infarction size was evaluated and the neurons and neuroglia in the perifocal zone were counted. APC reduced the infarction size 2.5 times in comparison with the control and reduced by 16% the neuronal death in the perifocal zone layer V, causing no appreciable changes in layer III, and did not change the size of neuronal bodies but increased (by 11%) the size of neuronal nuclei in layer III. The protein maintained the sharply increased count of gliocytes in the perifocal zone of infarction and promoted their growth. Hence, APC protected the neurons from death in the ischemic focus by increasing the gliocyte count and stimulating the compensatory reparative processes.
authors.affiliation: Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russia, rolfbrain@yandex.ru.
authors.forename: R M
authors.initials: RM
authors.lastname: Khudoerkov
authors.forename: I G
authors.initials: IG
authors.lastname: Savinkova
authors.forename: S M
authors.initials: SM
authors.lastname: Strukova
authors.forename: L R
authors.initials: LR
authors.lastname: Gorbacheva
authors.forename: M V
authors.initials: MV
authors.lastname: Gulyaev
authors.forename: Yu A
authors.initials: YA
authors.lastname: Pirogov
authors.forename: V N
authors.initials: VN
authors.lastname: Sal'kov
authors.forename: V B
authors.initials: VB
authors.lastname: Sobolev
authors.forename: S A
authors.initials: SA
authors.lastname: Gavrilova
authors.forename: V B
authors.initials: VB
authors.lastname: Koshelev
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D002552:Cerebral Ventricles / Q000473:pathology; D054059:Coronary Occlusion / Q000473:pathology; D007276:Injections, Intraventricular; D008297:Male; D020768:Middle Cerebral Artery / Q000473:pathology; D009457:Neuroglia / Q000187:drug effects* / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011486:Protein C / Q000819:agonists / Q000494:pharmacology*; D051381:Rats; D020521:Stroke / Q000188:drug therapy* / Q000473:pathology
chemical_list: D018696:Neuroprotective Agents; D011486:Protein C