title: Identification of biotin carboxyl carrier protein in Tetrahymena and its application in in vitro motility systems of outer arm dynein.
abstract: Axonemal dynein plays a central role in ciliary beating. Recently, a functional expression system of axonemal dynein was established in the ciliated protozoan Tetrahymena. This study identifies biotin carboxyl carrier protein (BCCP) in Tetrahymena and demonstrates its application in in vitro motility systems of outer arm dynein.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan. Electronic address: cedam@mail.ecc.u-tokyo.ac.jp.
authors.forename: Masaki
authors.initials: M
authors.lastname: Edamatsu
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000032:analysis* / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D016000:Cluster Analysis; D004398:Dyneins / Q000378:metabolism*; D054889:Fatty Acid Synthase, Type II / Q000032:analysis / Q000235:genetics; D008124:Locomotion; D008958:Models, Molecular; D010802:Phylogeny; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D013768:Tetrahymena / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*
chemical_list: D004398:Dyneins; D054889:Fatty Acid Synthase, Type II; D000103:Acetyl-CoA Carboxylase; C079633:biotin carboxyl carrier protein
keywords: Axonemal dynein; Biomolecular motor; Biotin carboxyl carrier protein (BCCP); Ciliary movement; Ciliated protozoan; Tetrahymena