title: Biomethane production and dynamics of microflora in response to copper treatments during mesophilic anaerobic digestion.
abstract: This study discussed the effects of different concentrations (0.625, 1.875 and 3.125 mM) of copper (Cu) in the form of CuSO4 on biomethane production and on the dynamics of microbial communities during the mesophilic anaerobic digestion (AD) of cow manure. The effects on biomethane production were found to depend on CuSO4 concentrations. After 50 days of AD, treatment A3 (3.125 mM) had lower cumulative biomethane production than the no-Cu control. The maximum value of cumulative biomethane production was detected under treatment A2 (1.875 mM). These results suggested that the stimulation or inhibition to biomethane production might be related to the concentration and chemical forms of Cu. Moreover, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was used to discuss the dynamics of microbial communities. Results revealed that different concentrations of CuSO4 had effects on the richness and diversity of bacterial and archaeal communities. The predominance of Bacteroidetes bacterium (GU339485.1) was verified through the sequencing of the dominant DGGE bands. Furthermore, Bacteroidetes bacterium could be detected during the whole AD process and is adaptable to a certain concentration range of CuSO4.
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, China kex@iae.ac.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Ke
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, China.
authors.forename: Chunyong
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, China.
authors.forename: Rundong
authors.initials: R
authors.lastname: Li
authors.affiliation: College of Land and Environment, Shenyang Agriculture University, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, China.
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Gui
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D000818:Animals; D001105:Archaea / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels / Q000032:analysis*; D019149:Bioreactors / Q000382:microbiology; D002417:Cattle; D019327:Copper Sulfate / Q000378:metabolism*; D058645:Denaturing Gradient Gel Electrophoresis; D004305:Dose-Response Relationship, Drug; D005285:Fermentation; D008372:Manure / Q000382:microbiology; D008697:Methane / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D056804:Biofuels; D008372:Manure; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D019327:Copper Sulfate; D008697:Methane
keywords: Copper; PCR-DGGE; anaerobic digestion (AD); archaeal community; bacterial community; mesophilic