title: Resistin 420C/G gene polymorphism on circulating resistin, metabolic risk factors and insulin resistance in adult women.
abstract: OBJECTIVE
  To investigate the frequency association between resistin gene polymorphism with its circulating levels, metabolic risk factor and insulin resistance in adult women.
  
  
  METHODS
  Totally 615 subjects were enrolled for the study, 305 women were with metabolic syndrome and 310 women were without metabolic syndrome according to NCEP-ATP III criteria. Fasting circulatory level of resistin, insulin, plasma glucose and lipid profiles were estimated along with calculation of insulin resistance. Resistin 420C/G promoter region polymorphism was done by RFLP method.
  
  
  RESULTS
  Variant genotype (CC vs CG+GG) (p<0.001: OR=2.22: 95% CI=1.60-3.10) of 420C/G resistin gene polymorphism was less frequently observed in control population. Further dividing subjects into two groups according to absence (Resistin -1) or presence (Resistin-2) of the G allele, significantly high levels of triglyceride (p<0.001), plasma glucose (p=0.012), systolic blood pressure (p<0.001), diastolic blood pressure (p<0.001), waist hip ratio (p<0.001), body mass index (p<0.001) and resistin (p<0.001), were observed in resistin-2 group.
  
  
  CONCLUSIONS
  Present study shows that 420C/G polymorphism of resistin gene directly correlated to its high circulating level and metabolic risk factors, specifically markers of obesity and atherosclerosis, so it may have an important role in the development of metabolic syndrome and cardio metabolic diseases.
authors.affiliation: Department of Physiology, King George's Medical University, Lucknow, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Physiology, King George's Medical University, Lucknow, India. Electronic address: vaniresearch@rediffmail.com.
authors.forename: Vani
authors.initials: V
authors.lastname: Gupta
authors.affiliation: Department of Physiology, King George's Medical University, Lucknow, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Srivastava
authors.affiliation: Department of Obstetrics and Gynecology, RIMS, Saifai, Etawah, India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Gupta
authors.affiliation: Department of Physiology, King George's Medical University, Lucknow, India.
authors.forename: Sameeksha
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Department of Physiology, King George's Medical University, Lucknow, India.
authors.forename: Supriya
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Department of Chemical Pathology, King George's Medical University, Lucknow, India.
authors.forename: Madhav
authors.initials: M
authors.lastname: Natu Shankar
authors.affiliation: Department of Biochemistry, Central Drug Research Institute, Lucknow, India.
authors.forename: Uma
authors.initials: U
authors.lastname: Roy
authors.affiliation: Department of Gastroenterology, King George's Medical University, Lucknow, India.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chandra
authors.affiliation: Institute for Data Computing & Training, Lucknow, India.
authors.forename: M P S
authors.initials: MP
authors.lastname: Negi
authors.affiliation: All India Institute of Medical Sciences, Bhopal, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000328:Adult; D000483:Alleles; D050197:Atherosclerosis / Q000097:blood / Q000235:genetics / Q000276:immunology; D001794:Blood Pressure; D005260:Female; D006801:Humans; D024821:Metabolic Syndrome / Q000097:blood / Q000235:genetics* / Q000276:immunology; D009765:Obesity / Q000097:blood / Q000235:genetics / Q000276:immunology; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D052243:Resistin / Q000097:blood / Q000235:genetics* / Q000276:immunology; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood / Q000276:immunology
chemical_list: C499624:RETN protein, human; D052243:Resistin; D014280:Triglycerides
keywords: Insulin resistance; Metabolic syndrome; Resistin level; Resistin promoter gene polymorphism