title: Monitoring bisphenol A and its biodegradation in water using a fluorescent molecularly imprinted chemosensor.
abstract: In this paper, we present a simple and rapid method for monitoring bisphenol A (BPA) and its biodegradation in environmental water using a fluorescent molecularly imprinted polymer chemosensor (fMIPcs). A fluorescent molecularly imprinted polymer (fMIP) was first synthesized by precipitation polymerization method using BPA as template, dansyl methacrylate as functional monomer. Then a fMIPcs was constructed by combining the fMIP with a fluorescent microplate reader. The fMIPcs displayed selective, concentration-dependent fluorescence quenching in response to BPA in water even in the existence of interferences, thereby allowing reliable high through-put quantification of BPA via simple fluorescence measurements. The fMIPcs was able to directly quantify BPA (from 10 to 2000 μg L(-1)) in different environmental water samples (distilled water, distilled water containing heavy metals and humic acid, tap water, and river water) with high accuracy, and to monitor BPA biodegradation in real-time. Using the fMIPcs, it was possible to achieve fast analytical results with lower limit of detection for BPA (3 μg L(-1)) from smaller sample volume (250 μL), which are superior to many relevant methods reported in the literature. Moreover, BPA levels and biodegradation rates measured by fMIPcs are comparable to the instrument-based method (HPLC). The fMIPcs developed in this work offers a new solution for simple, rapid, accurate and high through-put BPA quantification, and makes it possible to monitor BPA biodegradation in real time.
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address: cherrywu521@gmail.com.
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Wu
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Jingjiang Waterway Regulation Works Construction Headquarter, Changjiang Waterway Bureau, #99 Jiangjin Road, Jingzhou, Hubei 434001, China. Electronic address: 729789596@qq.com.
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: Department of Public Health, Xinxiang Medical University, East Jin Sui Road, Xinxiang, Henan 453003, China. Electronic address: 452421211@qq.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
authors.affiliation: Jingjiang Waterway Regulation Works Construction Headquarter, Changjiang Waterway Bureau, #99 Jiangjin Road, Jingzhou, Hubei 434001, China. Electronic address: 1185517627@qq.com.
authors.forename: Kai-Chun
authors.initials: KC
authors.lastname: Gao
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address: xiahu19872006@126.com.
authors.forename: Hu
authors.initials: H
authors.lastname: Xia
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address: lmfqs@163.com.
authors.forename: Mi-Fang
authors.initials: MF
authors.lastname: Luo
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address: wxj_169@163.com.
authors.forename: Xue-Juan
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Division of Pure and Applied Biochemistry, Lund University, Box 124, SE 22 100 Lund, Sweden. Electronic address: Lei.Ye@tbiokem.lth.se.
authors.forename: Lei
authors.initials: L
authors.lastname: Ye
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address: yunshi31@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address: lubin@mails.tjmu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis* / Q000378:metabolism; D001673:Biodegradation, Environmental; D004784:Environmental Monitoring / Q000379:methods*; D005453:Fluorescence; D005618:Fresh Water / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D008689:Methacrylates; D010636:Phenols; D011108:Polymers; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Biodegradation; Bisphenol A; Fluorescent chemosensor; Microplate reader; Molecularly imprinted polymer; Real-time monitoring