title: Effect of Hominis Placenta on cutaneous wound healing in normal and diabetic mice.
abstract: OBJECTIVE
  The number of diabetic patients has recently shown a rapid increase, and delayed wound healing is a major clinical complication in diabetes. In this study, the wound healing effect of Hominis placenta (HP) treatment was investigated in normal and streptozotocin-induced diabetic mice.
  
  
  METHODS
  Four full thickness wounds were created using a 4 mm biopsy punch on the dorsum. HP was injected subcutaneously at the middle region of the upper and lower wounds. Wounds were digitally photographed and wound size was measured every other day until the 14th day. Wound closure rate was analyzed using CANVAS 7SE software. Wound tissues were collected on days 2, 6, and 14 after wounding for H/E, immunohistochemistry for FGF2, and Masson's trichrome staining for collagen study.
  
  
  RESULTS
  Significantly faster wound closure rates were observed in the HP treated group than in normal and diabetes control mice on days 6 and 8. Treatment with HP resulted in reduced localization of inflammatory cells in wounded skin at day 6 in normal mice and at day 14 in diabetic mice (P < 0.01). Expression of fibroblast growth factor (FGF) 2 showed a significant increase in the HP treated group on day 14 in both normal (P < 0.01) and diabetic mice (P < 0.05). In addition, HP treated groups showed a thicker collagen layer than no treatment groups, which was remarkable on the last day, day 14, in both normal and diabetic mice.
  
  
  CONCLUSIONS
  Taken together, HP treatment has a beneficial effect on acceleration of cutaneous wound healing via regulation of the entire wound healing process, including inflammation, proliferation, and remodeling.
authors.affiliation: Studies of Translational Acupuncture Research, Acupuncture and Meridian Science Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea. ; Department of Korean Medical Science, Graduate School of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea.
authors.forename: Ji-Yeun
authors.initials: JY
authors.lastname: Park
authors.affiliation: Studies of Translational Acupuncture Research, Acupuncture and Meridian Science Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Lee
authors.affiliation: Happy Kyung Hee Korean Medicine Clinic, Banrim-dong, Sungsan-gu, Changwon, Kyungbuk 642-180, Korea.
authors.forename: Minsu
authors.initials: M
authors.lastname: Jeong
authors.affiliation: Studies of Translational Acupuncture Research, Acupuncture and Meridian Science Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea. ; Department of Korean Medical Science, Graduate School of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea.
authors.forename: Seorim
authors.initials: S
authors.lastname: Min
authors.affiliation: Studies of Translational Acupuncture Research, Acupuncture and Meridian Science Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea.
authors.forename: Song-Yi
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Studies of Translational Acupuncture Research, Acupuncture and Meridian Science Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea. ; Department of Korean Medical Science, Graduate School of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea.
authors.forename: Hyejung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea.
authors.forename: Yunsook
authors.initials: Y
authors.lastname: Lim
authors.affiliation: Studies of Translational Acupuncture Research, Acupuncture and Meridian Science Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea. ; Department of Korean Medical Science, Graduate School of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea.
authors.forename: Hi-Joon
authors.initials: HJ
authors.lastname: Park
keywords: Diabetes; Hominis placenta; fibroblast growth factor 2; inflammation; wound
references.citation: J Am Coll Surg. 1996 Jul;183(1):61-4
references.pmid: 8673309
references.citation: Growth Factors. 2004 Jun;22(2):111-9
references.pmid: 15253387
references.citation: Biol Pharm Bull. 2003 Oct;26(10):1472-7
references.pmid: 14519957
references.citation: Diabetes Res Clin Pract. 2005 Jan;67(1):3-21
references.pmid: 15620429
references.citation: Diabetes. 1988 Apr;37(4):371-6
references.pmid: 3378683
references.citation: J Clin Invest. 2010 Dec;120(12):4207-19
references.pmid: 21060152
references.citation: Clin Plast Surg. 2003 Jan;30(1):37-45
references.pmid: 12636214
references.citation: Microsc Res Tech. 2003 Jan 1;60(1):107-14
references.pmid: 12500267
references.citation: J Surg Res. 2002 Nov;108(1):122-8
references.pmid: 12443724
references.citation: Am J Surg. 2002 Apr;183(4):406-12
references.pmid: 11975928
references.citation: Biomed Res Int. 2013;2013:385641
references.pmid: 23653894
references.citation: Am J Pathol. 1990 Jun;136(6):1235-46
references.pmid: 2356856
references.citation: Wound Repair Regen. 2009 Jul-Aug;17(4):461-72
references.pmid: 19614910
references.citation: Acta Pharmacol Sin. 2006 Jan;27(1):50-8
references.pmid: 16364210
references.citation: Clin Plast Surg. 1990 Jul;17(3):493-501
references.pmid: 2199140
references.citation: J Ethnopharmacol. 2013 May 20;147(2):286-92
references.pmid: 23500882
references.citation: Front Biosci. 2004 Jan 01;9:283-9
references.pmid: 14766366
references.citation: Nat Prod Res. 2011 Dec;25(20):1988-92
references.pmid: 21707226
references.citation: Nature. 2008 May 15;453(7193):314-21
references.pmid: 18480812
references.citation: Exp Biol Med (Maywood). 2013 Jul;238(7):733-43
references.pmid: 23788174
references.citation: Lancet. 2005 Nov 12;366(9498):1736-43
references.pmid: 16291068
references.citation: Proc Natl Acad Sci U S A. 1986 Oct;83(19):7297-301
references.pmid: 2429303
references.citation: Dermatol Surg. 2005 Jun;31(6):674-86; discussion 686
references.pmid: 15996419
references.citation: Wound Repair Regen. 1998 Mar-Apr;6(2):135-41
references.pmid: 9776856
references.citation: J Exp Med. 1998 Feb 2;187(3):297-306
references.pmid: 9449710
references.citation: J Cell Physiol. 2011 Aug;226(8):2033-40
references.pmid: 21520055
references.citation: Photomed Laser Surg. 2012 Nov;30(11):617-36
references.pmid: 23075146
references.citation: Wound Repair Regen. 1995 Oct-Dec;3(4):408-18
references.pmid: 17147652
references.citation: PLoS One. 2013;8(3):e59585
references.pmid: 23555718
references.citation: Nature. 1987 Jan 15-21;325(6101):257-9
references.pmid: 2433585
references.citation: J Oral Maxillofac Surg. 1988 May;46(5):391-8
references.pmid: 2452866
references.citation: Photomed Laser Surg. 2013 Jul;31(7):315-21
references.pmid: 23789588
references.citation: Am J Pathol. 2007 Apr;170(4):1178-91
references.pmid: 17392158
references.citation: Lancet. 1980 Jul 5;2(8184):40
references.pmid: 6104256
references.citation: Ann Plast Surg. 2010 Jul;65(1):96-100
references.pmid: 20548228
references.citation: Placenta. 2010 Oct;31(10):873-9
references.pmid: 20708262
references.citation: Evid Based Complement Alternat Med. 2011;2011:
references.pmid: 20953385