title: [Amplification efficency and optimization of culture conditions of γδ T cells in peripheral blood by different phosphate compounds].
abstract: OBJECTIVE
  To compare the efficiency of pamidronate (PAM) and isopentenyl pyrophosphate (IPP) to stimulate γδ T cell expansion from human peripheral blood and explore the optimized expansion conditions.
  
  
  METHODS
  Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque gradient centrifugation, and then cultured in RPMI1640 medium supplemented with 10% fetal bovine serum, IPP (1.0, 5.0, 10.0, 15.0 μg/mL) or PAM (2.0, 5.0, 8.0, 12.0 μg/mL), and IL-2 (100.0, 200.0, 500.0 IU/mL). The cells were observed and collected. The number and proportion of CD3⁺TCRδ2⁺ γδ T cells stimulated by PAM or IPP in total lymphocytes were evaluated by flow cytometry and the expansion efficiency was calculated.
  
  
  RESULTS
  After 14 days, the ratios of γδ T cells in total lymphocytes in IPP group and PAM group increased to 81.3% and 78.5%, respectively. This indicated that both IPP and PAM could effectively stimulate γδ T cell expansion and there was no significant difference in the efficiency of expansion between the two groups (P>0.05).
  
  
  CONCLUSIONS
  PAM has the similar ability with IPP to stimulate γδ T cell expansion in vitro. PAM could become more economical and practical choice for stimulating γδ T cell expansion.
authors.affiliation: Chengdu Blood Center, Sichuan University, Chengdu 610041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xi
authors.affiliation: West China School of Medicine, Sichuan University, Sichuan University, Chengdu 610041, China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Miao
authors.affiliation: Chengdu Blood Center, Sichuan University, Chengdu 610041, China.
authors.forename: Like
authors.initials: L
authors.lastname: Wan
authors.affiliation: Chengdu Blood Center, Sichuan University, Chengdu 610041, China.
authors.forename: Yuanhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: West China School of Medicine, Sichuan University, Sichuan University, Chengdu 610041, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Feng
authors.affiliation: Chengdu Blood Center, Sichuan University, Chengdu 610041, China.
authors.forename: Tianxiang
authors.initials: T
authors.lastname: Gong
authors.affiliation: West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Li
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004164:Diphosphonates / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D045782:Hemiterpenes / Q000494:pharmacology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D000077268:Pamidronate; D010710:Phosphates / Q000494:pharmacology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D004164:Diphosphonates; D045782:Hemiterpenes; D007376:Interleukin-2; D009943:Organophosphorus Compounds; D010710:Phosphates; D016692:Receptors, Antigen, T-Cell, gamma-delta; C004809:isopentenyl pyrophosphate; D000077268:Pamidronate