title: Prevention of Staphylococcus epidermidis biofilm formation using electrical current.
abstract: A technique for the prevention of staphylococcal adhesion by electrical current exposure was investigated. Teflon coupons were exposed to a continuous flow of 103 cfu/ml Staphylococcus epidermidis with or without 2000 microA DC electrical current delivered by electrodes on opposite sides of a coupon, touching neither each other nor the coupon. A mean 3.46 (SD, 0.20) and 5.70 (SD, 1.03) log10 cfu/cm2 were adhered to the non-electrical current exposed coupons after 4 h and 24 h, respectively. A mean 2.46 (SD, 0.31) and 1.47 (SD, 0.73) log10 cfu/cm2 were adhered after 4 h and 24 h with exposure to 2000 microA electrical current delivered by graphite electrodes. A mean 2.21 (SD, 0.14) and 0.55 (SD, 0.00) log10 cfu/cm2 were adhered after 4 h and 24 h with exposure to 2000 microA electrical current delivered by stainless steel electrodes. Electrical current may be useful in the prevention of staphylococcal adhesion to biomaterials.
authors.affiliation: 2 Infectious Diseases Research Laboratory, Mayo Clinic College of Medicine, Rochester, Minnesota - USA.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Del Pozo
authors.forename: Mark S
authors.initials: MS
authors.lastname: Rouse
authors.forename: Gorane
authors.initials: G
authors.lastname: Euba
authors.forename: Kerryl E
authors.initials: KE
authors.lastname: Greenwood-Quaintance
authors.forename: Jayawant N
authors.initials: JN
authors.lastname: Mandrekar
authors.forename: James M
authors.initials: JM
authors.lastname: Steckelberg
authors.forename: Robin
authors.initials: R
authors.lastname: Patel
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D004560:Electricity; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D013193:Stainless Steel / Q000737:chemistry; D013212:Staphylococcus epidermidis / Q000502:physiology*
chemical_list: D013193:Stainless Steel; D006108:Graphite
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