title: Repetitive magnetic stimulation induces plasticity of excitatory postsynapses on proximal dendrites of cultured mouse CA1 pyramidal neurons.
abstract: Repetitive transcranial magnetic stimulation (rTMS) of the human brain can lead to long-lasting changes in cortical excitability. However, the cellular and molecular mechanisms which underlie rTMS-induced plasticity remain incompletely understood. Here, we used repetitive magnetic stimulation (rMS) of mouse entorhino-hippocampal slice cultures to study rMS-induced plasticity of excitatory postsynapses. By employing whole-cell patch-clamp recordings of CA1 pyramidal neurons, local electrical stimulations, immunostainings for the glutamate receptor subunit GluA1 and compartmental modeling, we found evidence for a preferential potentiation of excitatory synapses on proximal dendrites of CA1 neurons (2-4 h after stimulation). This rMS-induced synaptic potentiation required the activation of voltage-gated sodium channels, L-type voltage-gated calcium channels and N-methyl-D-aspartate-receptors. In view of these findings we propose a cellular model for the preferential strengthening of excitatory synapses on proximal dendrites following rMS in vitro, which is based on a cooperative effect of synaptic glutamatergic transmission and postsynaptic depolarization.
authors.affiliation: Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Lenz
authors.affiliation: Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Platschek
authors.affiliation: Department of Neural Systems and Coding, Max Planck Institute for Brain Research, Frankfurt/Main, Germany.|Department of Nonlinear Dynamics, Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany.
authors.forename: Viola
authors.initials: V
authors.lastname: Priesemann
authors.affiliation: Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany.
authors.forename: Denise
authors.initials: D
authors.lastname: Becker
authors.affiliation: Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany.
authors.forename: Laurent M
authors.initials: LM
authors.lastname: Willems
authors.affiliation: Department of Neurology and Stroke, and Hertie Institute for Clinical Brain Research, Eberhard-Karls-University, Tübingen, Germany.
authors.forename: Ulf
authors.initials: U
authors.lastname: Ziemann
authors.affiliation: Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Deller
authors.affiliation: Department of Neurology and Stroke, and Hertie Institute for Clinical Brain Research, Eberhard-Karls-University, Tübingen, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Müller-Dahlhaus
authors.affiliation: Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Jedlicka
authors.affiliation: Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany. a.vlachos@med.uni-frankfurt.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Vlachos
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000166:cytology / Q000502:physiology*; D002478:Cells, Cultured; D003712:Dendrites / Q000502:physiology*; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D009473:Neuronal Plasticity / Q000502:physiology*; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000502:physiology*; D017470:Receptors, Glutamate / Q000502:physiology; D013569:Synapses / Q000502:physiology; D050781:Transcranial Magnetic Stimulation / Q000379:methods*
chemical_list: D017470:Receptors, Glutamate
keywords: 3D-reconstruction; AMPA-receptors; Backpropagating action potentials; Computational modelling; Hebbian plasticity; Silent synapses; Spike timing dependent plasticity; Strontium