title: Qualitative and quantitative peptidomic and proteomic approaches to phenotyping chicken semen.
abstract: Understanding of the avian male gamete biology is essential to improve the conservation of genetic resources and performance in farming. In this study, the chicken semen peptidome/proteome and the molecular phenotype related to sperm quality were investigated. Spermatozoa (SPZ) and corresponding seminal plasma (SP) from 11 males with different fertilizing capacity were analyzed using three quantitative strategies (fluid and intact cells MALDI-MS, SDS-PAGE combined to LC-MS/MS with spectral counting and XIC methods). Individual MALDI profiling in combination with top-down MS allowed to characterize specific profiles per male and to identify 16 biomolecules (e.g.VMO1, AvBD10 and AvBD9 including polymorphism). Qualitative analysis identified 1165 proteins mainly involved in oxidoreduction mechanisms, energy processes, proteolysis and protein localization. Comparative analyses between the most and the least fertile males were performed. The enzymes involved in energy metabolism, respiratory chain or oxido-reduction activity were over-represented in SPZ of the most fertile males. The SP of the most and the least fertile males differed also on many proteins (e.g. ACE, AvBD10 and AvBD9, NEL precursor, acrosin). Thus proteomic is a "phenomic molecular tool" that may help to discriminate avian males on their reproductive capacity. The data have been deposited with ProteomeXchange (identifiers PXD000287 and PXD001254).
  
  
  CONCLUSIONS
  This peptidomic and proteomic study i) characterized for the first time the semen protein composition of the main domestic avian species (Gallus gallus) by analysis of ejaculated spermatozoa and corresponding seminal plasma; ii) established a characteristic molecular phenotype distinguishing semen and males at an individual level; and iii) proposedthe first evidence of biomarkers related to fertility.
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; INRA, Plate-forme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Labas
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Grasseau
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Cahier
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Gargaros
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France; INRA, Plate-forme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France.
authors.forename: Grégoire
authors.initials: G
authors.lastname: Harichaux
authors.affiliation: INRA, Plate-forme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, F-37380 Nouzilly, France; INRA, UMR 1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France; Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, F-37000 Tours, France.
authors.forename: Ana-Paula
authors.initials: AP
authors.lastname: Teixeira-Gomes
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Alves
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Bourin
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Gérard
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, Institut Français du Cheval et de l'Equitation, F-37380 Nouzilly, France. Electronic address: Elisabeth.Blesbois@tours.inra.fr.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Blesbois
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000378:metabolism*; D002645:Chickens; D008297:Male; D040901:Proteomics; D012661:Semen / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; D029589:Seminal Plasma Proteins
keywords: Chicken (Gallus gallus); Fertility; ICM-MS; Proteomic; Semen; Top-down MS