title: Glycoprotein profiling of stem cells using lectin microarray based on surface plasmon resonance imaging.
abstract: Lectin microarrays have emerged as a novel platform for glycan analysis during recent years. Here, we have combined surface plasmon resonance imaging (SPRi) with the lectin microarray for rapid and label-free profiling of stem cells. In this direction, 40 lectins from seven different glyco-binding motifs and three different cell lines-mouse embryonic stem cells (mESCs), mouse-induced pluripotent stem cells (miPSCs), and mouse embryonic fibroblast stem cells (MEFs)-were used. Pluripotent mouse stem cells were clearly distinguished from non-pluripotent stem cells. Eight lectins-DBA, MAL, PHA_E, PHA_L, EEL, AAL, PNA, and SNA-generated maximal value to define pluripotency of mouse stem cells in our experiments. The discriminant function based on lectin reactivities was highly accurate for the determination of stem cell pluripotency. These results suggested that glycomic analysis of stem cells leads to a novel comprehensive approach for quality control in cell-based therapy and regenerative medicine.
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Amita
authors.initials: A
authors.lastname: Nand
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Vikramjeet
authors.initials: V
authors.lastname: Singh
authors.affiliation: Centre for Stem Cell Biology and Regenerative Medicine, School of Medicine, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Peizhe
authors.initials: P
authors.lastname: Wang
authors.affiliation: Centre for Stem Cell Biology and Regenerative Medicine, School of Medicine, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Na
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China. Electronic address: jizhu@nanoctr.cn.
authors.forename: Jingsong
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002460:Cell Line; D037102:Lectins / Q000737:chemistry*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D037102:Lectins
keywords: Glycoprotein; Lectin microarray; Pluripotency; SPRi; Stem cell