title: MicroRNA-92 promotes gastric cancer cell proliferation and invasion through targeting FXR.
abstract: MicroRNAs (miRNAs), a class of small noncoding RNAs, play critical roles in human carcinogenesis through downregulation of various target genes. In the present study, we found that miR-92 is upregulated in gastric cancer tissues compared with adjacent normal tissues. Interestingly, miR-92 expression is significantly associated with clinical characteristics of patients. Gain or loss-of-function in vitro experiments further show that miR-92 mimics significantly promoted, while its antisense oligos inhibited gastric cancer cell proliferation and invasion. Moreover, luciferase reporter assays and western blot indicated that farnesoid X receptor (FXR), is a direct target of miR-92. Therefore, our data suggest that upregulation of miR-92 may represent an important mechanism for the development of gastric cancer.
authors.affiliation: Department of Gastroenterology, Second Hospital of Shaoxing City, Shaoxing, Zhejiang Province, 312000, China, jianhuaduan@126.com.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Duan
authors.forename: Long
authors.initials: L
authors.lastname: Fang
mesh_terms: D000328:Adult; D017209:Apoptosis; D015153:Blotting, Western; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D018160:Receptors, Cytoplasmic and Nuclear / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured; D000099150:Receptor, Farnesoid X-Activated
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018160:Receptors, Cytoplasmic and Nuclear; D000099150:Receptor, Farnesoid X-Activated; C541874:MIRN92 microRNA, human
references.citation: Int J Immunopathol Pharmacol. 2010 Oct-Dec;23(4):1229-34
references.pmid: 21244772
references.citation: Gut. 2014 Jan;63(1):170-8
references.pmid: 23348961
references.citation: Nat Rev Mol Cell Biol. 2013 Aug;14(8):475-88
references.pmid: 23800994
references.citation: Cancer Res. 2010 Jun 1;70(11):4778-84
references.pmid: 20484043
references.citation: Carcinogenesis. 2011 Jul;32(7):1005-12
references.pmid: 21572098
references.citation: World J Gastroenterol. 2011 Oct 21;17(39):4421-8
references.pmid: 22110269
references.citation: Cell. 2000 Sep 15;102(6):731-44
references.pmid: 11030617
references.citation: Oncol Rep. 2012 Apr;27(4):1019-26
references.pmid: 22267008
references.citation: Oncol Lett. 2013 Aug;6(2):468-474
references.pmid: 24137349
references.citation: J Clin Invest. 2004 May;113(10):1408-18
references.pmid: 15146238
references.citation: Cancer Sci. 2011 Dec;102(12):2264-71
references.pmid: 21883694
references.citation: Nat Rev Gastroenterol Hepatol. 2013 Sep;10(9):542-52
references.pmid: 23689081
references.citation: BMC Cancer. 2010 Jul 12;10:367
references.pmid: 20618998
references.citation: J Clin Invest. 2006 Apr;116(4):1102-9
references.pmid: 16557297
references.citation: Nat Rev Genet. 2013 Aug;14(8):535-48
references.pmid: 23817310
references.citation: FEBS Lett. 2014 Feb 14;588(4):538-44
references.pmid: 24374340
references.citation: Epigenetics. 2010 Oct 1;5(7):656-63
references.pmid: 20716963
references.citation: Nat Rev Genet. 2012 Apr 18;13(5):358-69
references.pmid: 22510765
references.citation: Cancer Res. 2008 Dec 1;68(23):9589-94
references.pmid: 19047134
references.citation: Nat Rev Mol Cell Biol. 2012 Mar 22;13(4):239-50
references.pmid: 22436747
references.citation: Cancer Res. 2006 Oct 15;66(20):10120-6
references.pmid: 17047076
references.citation: Nat Rev Drug Discov. 2013 Nov;12(11):847-65
references.pmid: 24172333
references.citation: Cancer Lett. 2012 Oct 28;323(2):215-22
references.pmid: 22579649
references.citation: Biochem J. 2011 Sep 1;438(2):315-23
references.pmid: 21619550
references.citation: Nat Rev Clin Oncol. 2013 Nov;10(11):643-55
references.pmid: 24061039
references.citation: Cancer Res. 2007 Feb 1;67(3):863-7
references.pmid: 17283114