title: Molecular identification of chlamydial cause of abortion in small ruminants in Jordan.
abstract: Chlamydophila abortus (Ch. abortus) is the etiological agent of ovine enzootic abortion (OEA) and one of the most common infectious agents of abortion in small ruminants worldwide. RFLP-PCR analysis of the outer membrane protein gene (OMP2 gene) was used for diagnosis and characterization of chlamydial causes of abortion in small ruminants in Jordan. Sixty-six placental tissues and 15 vaginal swabs were collected from aborted ewes and does to identify cause of abortion in Jordan. Thirty-eight placental samples (58 %) and 13 vaginal swabs (87 %) were positive for chlamydial DNA. Shedding of bacteria in vaginal swabs was detected within 7 days after abortion. The results of this study showed that chlamydiosis is one of the important causes of abortion in small ruminants in Jordan. In addition, vaginal swab is an excellent sample for molecular diagnosis of chlamydiosis. DNA sequencing and RFLP analysis of the OMP2 reveal that all chlamydial cause of abortion in small ruminants in Jordan are due to Ch. abortus. While, Ch. pecorum was not detected in any sample. OMP2 gene of the isolated Jordanian strain was identical (100 %) to Ch. abortus FAS strain. In conclusion, Ch. abortus is an important cause of abortion in Jordan; vaginal swab within 7 days of abortion can be used for molecular diagnosis of chlamydiosis in small ruminants.
authors.affiliation: National Center for Agricultural Research and Extension, Baqa', 19381, Jordan, hothaifaababneh@yahoo.com.
authors.forename: Huthaifa Salah
authors.initials: HS
authors.lastname: Ababneh
authors.forename: Mustafa Mohammed Kheir
authors.initials: MM
authors.lastname: Ababneh
authors.forename: Wael Mahmoud
authors.initials: WM
authors.lastname: Hananeh
authors.forename: Fawzi Mohammad
authors.initials: FM
authors.lastname: Alsheyab
authors.forename: Khaleel Ibraheem
authors.initials: KI
authors.lastname: Jawasreh
authors.forename: Moath Ahmad
authors.initials: MA
authors.lastname: Al-Gharaibeh
authors.forename: Mohammed Mahmoud
authors.initials: MM
authors.lastname: Ababneh
mesh_terms: D000034:Abortion, Veterinary / Q000453:epidemiology / Q000382:microbiology*; D000818:Animals; D023501:Chlamydophila / Q000302:isolation & purification; D023521:Chlamydophila Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D015511:Goat Diseases / Q000453:epidemiology / Q000382:microbiology*; D006041:Goats; D007597:Jordan / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*; D014621:Vagina / Q000382:microbiology
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