title: Collagen scaffolds combined with collagen-binding ciliary neurotrophic factor facilitate facial nerve repair in mini-pigs.
abstract: The preclinical studies using animal models play a very important role in the evaluation of facial nerve regeneration. Good models need to recapitulate the distance and time for axons to regenerate in humans. Compared with the most used rodent animals, the structure of facial nerve in mini-pigs shares more similarities with humans in microanatomy. To evaluate the feasibility of repairing facial nerve defects by collagen scaffolds combined with ciliary neurotrophic factor (CNTF), 10-mm-long gaps were made in the buccal branch of mini-pigs' facial nerve. Three months after surgery, electrophysiological assessment and histological examination were performed to evaluate facial nerve regeneration. Immunohistochemistry and transmission electron microscope observation showed that collagen scaffolds with collagen binding (CBD)-CNTF could promote better axon regeneration, Schwann cell migration, and remyelination at the site of implant device than using scaffolds alone. Electrophysiological assessment also showed higher recovery rate in the CNTF group. In summary, combination of collagen scaffolds and CBD-CNTF showed promising effects on facial nerve regeneration in mini-pig models.
authors.affiliation: Department of VIP Service, School of Stomatology, Capital Medical University, Beijing, 10050, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.forename: Danqing
authors.initials: D
authors.lastname: Meng
authors.forename: Jiani
authors.initials: J
authors.lastname: Cao
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Yi
authors.initials: Y
authors.lastname: Cui
authors.forename: Jingya
authors.initials: J
authors.lastname: Fan
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Cui
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jinling
authors.initials: J
authors.lastname: Ma
authors.forename: Juli
authors.initials: J
authors.lastname: Pan
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D002450:Cell Communication / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D055724:Electrophysiological Phenomena / Q000187:drug effects; D005154:Facial Nerve / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology / Q000637:transplantation; D005260:Female; D006801:Humans; D009186:Myelin Sheath / Q000378:metabolism; D009416:Nerve Regeneration / Q000187:drug effects*; D011485:Protein Binding / Q000187:drug effects; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D013552:Swine; D013556:Swine, Miniature; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020934:Ciliary Neurotrophic Factor; D003094:Collagen
keywords: ciliaryneurotrophic factor; collagen scaffold; facial nerve regeneration; mini-pig