title: Using structure to inform carbohydrate binding module function.
abstract: Generally, non-catalytic carbohydrate binding module (CBM) specificity has been shown to parallel the catalytic activity of the carbohydrate active enzyme (CAZyme) module it is appended to. With the rapid expansion in metagenomic sequence space for the potential discovery of new CBMs in addition to the recent emergence of several new CBM families that display diverse binding profiles and novel functions, elucidating the function of these protein modules has become a much more challenging task. This review summarizes several approaches that have been reported for using primary structure to inform CBM specificity and streamlining their biophysical characterization. In addition we discuss general trends in binding site architecture and several newly identified functions for CBMs. Streams of investigation that will facilitate the development and refinement of sequence-based prediction tools are suggested.
authors.affiliation: Lethbridge Research Centre, Agriculture and Agri-Food Canada, 5403-1st Ave, Lethbridge, AB, Canada T1J4B1. Electronic address: wade.abbott@agr.gc.ca.
authors.forename: D Wade
authors.initials: DW
authors.lastname: Abbott
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen 9747AG, The Netherlands.
authors.forename: Alicia Lammerts
authors.initials: AL
authors.lastname: van Bueren
mesh_terms: D001665:Binding Sites; D002241:Carbohydrates / Q000737:chemistry*; D017124:Conserved Sequence; D004798:Enzymes / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D010802:Phylogeny; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D011506:Proteins