title: Nandrolone decanoate is able to modulate proliferation and adhesion of myoblasts.
abstract: The search for a more efficient repair process of muscle injuries has become evident in clinical practice. The aim of the present study was to evaluate the effect of nandrolone decanoate (ND) on the proliferation, adhesion, and expression of myogenic regulatory factors (MRFs) in C2C12 cells.Methods. Cell proliferation and adhesion were assessed using an MTT assay. The expression of MRFs was assessed by real-time PCR.Results. ND applied at 10 or 25 µM concentration induced after 60 min an increase in adhesion, at 5 µM concentration induced after 5 days an increase in cell proliferation, and ND at 50 µM concentration led after 5 days to a decrease in cell proliferation in comparison with other groups. The steroid did not alter the expression of MRFs.Conclusions. The positive effects of ND regarding the proliferation and adhesion of C2C12 cells suggest that this steroid may have positive effects following a muscle injury.
authors.forename: E N
authors.initials: EN
authors.lastname: Oliveira
authors.forename: K P
authors.initials: KP
authors.lastname: Fernandes
authors.forename: C A
authors.initials: CA
authors.lastname: Silva
authors.forename: T S
authors.initials: TS
authors.lastname: Oliveira
authors.forename: J A
authors.initials: JA
authors.lastname: Junior
authors.forename: S K
authors.initials: SK
authors.lastname: Bussadori
authors.forename: A C
authors.initials: AC
authors.lastname: Renno
authors.forename: R A
authors.initials: RA
authors.lastname: Mesquita-Ferrari
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D024510:Muscle Development / Q000187:drug effects / Q000235:genetics; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D032446:Myoblasts / Q000187:drug effects* / Q000502:physiology; D018008:Myogenin / Q000235:genetics / Q000378:metabolism; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077603:Nandrolone Decanoate; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; C503801:Myog protein, mouse; D018008:Myogenin; D014157:Transcription Factors; D009277:Nandrolone; D000077603:Nandrolone Decanoate