title: Immobilization of catalase on electrospun PVA/PA6-Cu(II) nanofibrous membrane for the development of efficient and reusable enzyme membrane reactor.
abstract: In this study, a mat/membrane consisting of overlaid PVA/PA6-Cu(II) composite nanofibers was prepared via the electrospinning technique followed by coordination/chelation with Cu(II) ions; an enzyme of catalase (CAT) was then immobilized onto the PVA/PA6-Cu(II) nanofibrous membrane. The amount of immobilized catalase reached a high value of 64 ± 4.6 mg/g, while the kinetic parameters (Vmax and Km) of enzyme were 3774 μmol/mg·min and 41.13 mM, respectively. Furthermore, the thermal stability and storage stability of immobilized catalase were improved significantly. Thereafter, a plug-flow type of immobilized enzyme membrane reactor (IEMR) was assembled from the PVA/PA6-Cu(II)-CAT membrane. With the increase of operational pressure from 0.02 to 0.2 MPa, the flux value of IEMR increased from 0.20 ± 0.02 to 0.76 ± 0.04 L/m(2)·min, whereas the conversion ratio of H2O2 decreased slightly from 92 ± 2.5% to 87 ± 2.1%. After 5 repeating cycles, the production capacity of IEMR was merely decreased from 0.144 ± 0.006 to 0.102 ± 0.004 mol/m(2)·min. These results indicated that the assembled IEMR possessed high productivity and excellent reusability, suggesting that the IEMR based on electrospun PVA/PA6-Cu(II) nanofibrous membrane might have great potential for various applications, particularly those related to environmental protection.
authors.affiliation: Key Laboratory of Textile Fabric, College of Textiles and Clothing, Anhui Polytechnic University , Wuhu, Anhui 241000, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Feng
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Anfang
authors.initials: A
authors.lastname: Wei
authors.forename: Changlong
authors.initials: C
authors.lastname: Li
authors.forename: Qufu
authors.initials: Q
authors.lastname: Wei
authors.forename: Hao
authors.initials: H
authors.lastname: Fong
mesh_terms: D000818:Animals; D019149:Bioreactors; D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry; D002374:Catalase / Q000378:metabolism* / Q000648:ultrastructure; D002417:Cattle; D003300:Copper / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism / Q000648:ultrastructure; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D016062:Porosity; D059027:Recycling; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011108:Polymers; C009916:nylon 6; D002209:Caprolactam; D003300:Copper; D011142:Polyvinyl Alcohol; D006861:Hydrogen Peroxide; D002374:Catalase