title: A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes.
abstract: A novel optical detection system consisting of combination of uricase/HRP-CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125-1000 µM with detection limit of 125 µM.
authors.affiliation: Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia; Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.
authors.forename: Nur Ellina
authors.initials: NE
authors.lastname: Azmi
authors.affiliation: Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia.
authors.forename: Noor Izaanin
authors.initials: NI
authors.lastname: Ramli
authors.affiliation: Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. Electronic address: jafar@upm.edu.my.
authors.forename: Jaafar
authors.initials: J
authors.lastname: Abdullah
authors.affiliation: School of Applied Physics, Faculty of Sciences and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia.
authors.forename: Mohammad Azmi
authors.initials: MA
authors.lastname: Abdul Hamid
authors.affiliation: Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia.
authors.forename: Hamidah
authors.initials: H
authors.lastname: Sidek
authors.affiliation: Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.
authors.forename: Samsulida
authors.initials: S
authors.lastname: Abd Rahman
authors.affiliation: Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia.
authors.forename: Nurhayati
authors.initials: N
authors.lastname: Ariffin
authors.affiliation: Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.
authors.forename: Nor Azah
authors.initials: NA
authors.lastname: Yusof
mesh_terms: D015374:Biosensing Techniques; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000302:isolation & purification*; D045663:Quantum Dots; D014503:Urate Oxidase / Q000737:chemistry; D014527:Uric Acid / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine
chemical_list: D014527:Uric Acid; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D014503:Urate Oxidase
keywords: Enzyme; Fluorescence; Quantum dots; Quenching; Uric acid