title: Tandem mass spectrometry detection of quorum sensing activity in multidrug resistant clinical isolate Acinetobacter baumannii.
abstract: Many Proteobacteria communicate via production followed by response of quorum sensing molecules, namely, N-acyl homoserine lactones (AHLs). These molecules consist of a lactone moiety with N-acyl side chain with various chain lengths and degrees of saturation at C-3 position. AHL-dependent QS is often associated with regulation of diverse bacterial phenotypes including the expression of virulence factors. With the use of biosensor and high resolution liquid chromatography tandem mass spectrometry, the AHL production of clinical isolate A. baumannii 4KT was studied. Production of short chain AHL, namely, N-hexanoyl-homoserine lactone (C6-HSL) and N-octanoyl-homoserine lactone (C8-HSL), was detected.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Huey Jia
authors.initials: HJ
authors.lastname: Cheng
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Jian Woon
authors.initials: JW
authors.lastname: Chen
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yun Fong
authors.initials: YF
authors.lastname: Ngeow
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis; D040981:Acinetobacter baumannii / Q000378:metabolism / Q000502:physiology*; D018432:Drug Resistance, Multiple; D007783:Lactones; D053038:Quorum Sensing; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007783:Lactones; C088386:homoserine lactone; D015107:4-Butyrolactone
references.citation: FEMS Microbiol Lett. 2002 Jan 22;207(1):1-7
references.pmid: 11886742
references.citation: Curr Opin Microbiol. 2001 Apr;4(2):186-93
references.pmid: 11282475
references.citation: Antonie Van Leeuwenhoek. 2010 Oct;98(3):299-305
references.pmid: 20376561
references.citation: J Bacteriol. 2006 Jan;188(2):773-83
references.pmid: 16385066
references.citation: Sensors (Basel). 2012;12(4):4339-51
references.pmid: 22666033
references.citation: Annu Rev Microbiol. 1996;50:727-51
references.pmid: 8905097
references.citation: J Antimicrob Chemother. 2007 Jun;59(6):1210-5
references.pmid: 17324960
references.citation: Microbiol Res. 2001 Mar;155(4):271-7
references.pmid: 11297357
references.citation: Infect Control Hosp Epidemiol. 2002 May;23(5):261-7
references.pmid: 12026151
references.citation: ACS Chem Biol. 2012 Oct 19;7(10):1719-28
references.pmid: 22853441
references.citation: J Bacteriol. 2008 May;190(9):3386-92
references.pmid: 18281398
references.citation: J Clin Microbiol. 2008 Jun;46(6):1946-54
references.pmid: 18400920
references.citation: Sensors (Basel). 2012;12(4):4661-96
references.pmid: 22666051
references.citation: Microbiology (Reading). 1997 Dec;143 ( Pt 12):3703-3711
references.pmid: 9421896
references.citation: Sensors (Basel). 2013 May 13;13(5):6217-28
references.pmid: 23669710
references.citation: Sensors (Basel). 2012;12(4):4016-30
references.pmid: 22666015
references.citation: Philos Trans R Soc Lond B Biol Sci. 2000 May 29;355(1397):667-80
references.pmid: 10874739
references.citation: Annu Rev Genet. 2001;35:439-68
references.pmid: 11700290
references.citation: J Nat Prod. 2011 Oct 28;74(10):2261-4
references.pmid: 21910441
references.citation: BMC Microbiol. 2011 Mar 08;11:51
references.pmid: 21385437
references.citation: Int J Med Microbiol. 2006 Apr;296(2-3):149-61
references.pmid: 16503194
references.citation: Int J Med Microbiol. 2001 May;291(2):131-43
references.pmid: 11437336
references.citation: Clin Microbiol Rev. 2008 Jul;21(3):538-82
references.pmid: 18625687
references.citation: Clin Microbiol Infect. 2005 Nov;11(11):868-73
references.pmid: 16216100
references.citation: J Chemother. 2009 Jun;21(3):290-5
references.pmid: 19567349
references.citation: World J Microbiol Biotechnol. 2012 Feb;28(2):453-61
references.pmid: 22806840
references.citation: Microbiology (Reading). 2007 Dec;153(Pt 12):3923-3938
references.pmid: 18048907
references.citation: Trends Biochem Sci. 1996 Jun;21(6):214-9
references.pmid: 8744355
references.citation: Infect Immun. 2002 Oct;70(10):5635-46
references.pmid: 12228292
references.citation: Clin Infect Dis. 2010 Jun 15;50(12):1611-6
references.pmid: 20462357
references.citation: Nat Rev Microbiol. 2007 Dec;5(12):939-51
references.pmid: 18007677
references.citation: Microbiology (Reading). 2002 Apr;148(Pt 4):1119-1127
references.pmid: 11932456
references.citation: Curr Opin Microbiol. 1998 Apr;1(2):183-9
references.pmid: 10066485
references.citation: Philos Trans R Soc Lond B Biol Sci. 2007 Jul 29;362(1483):1119-34
references.pmid: 17360280
references.citation: Adv Microb Physiol. 2001;45:199-270
references.pmid: 11450110
references.citation: J Ind Microbiol Biotechnol. 2009 Feb;36(2):247-51
references.pmid: 18946694
references.citation: FEMS Microbiol Lett. 1998 Jun 15;163(2):185-92
references.pmid: 9673021
references.citation: Sensors (Basel). 2013 Apr 18;13(4):5117-29
references.pmid: 23598500
references.citation: Microbiology (Reading). 1995 Mar;141 ( Pt 3):541-50
references.pmid: 7711893
references.citation: J Hosp Infect. 2007 Mar;65(3):204-11
references.pmid: 17254667