title: Anti-psoriatic activity of Givotia rottleriformis in rats.
abstract: OBJECTIVE
  To evaluate the antipsoriatic activity of Givotia rottleriformis bark in rats.
  
  
  METHODS
  The antipsoriatic activity of the ethanol extract was investigated using ultraviolet B (UV-B)-induced photodermatitis model in rats. The animals were divided into four groups (6/groups). The vehicle-control group animals received normal saline (10 ml/kg, p.o.) and standard group received retinoic acid (0.5 mg/kg, p.o.). Remaining groups were treated orally with the ethanolic extract of bark of Givotia rottleriformis (200 and 400 mg/kg, p.o.) and data were analyzed using one-way analysis of variance (ANOVA). The extract was standardized using chemical markers by high-performance liquid chromatography (HPLC) analysis.
  
  
  RESULTS
  In case of psoriasis model, histopathological analysis revealed that in the section, there were absence of Munro's microabscess, elongation of rete ridges, and capillary loop dilation in ethanol extract (400 mg/Kg) and standard group. The ethanolic extract (200 and 400 mg/Kg) exhibited significant reduction (P < 0.01) in percentage of relative epidermal thickness as compared with positive control. In the HPLC analysis, 4 flavonoids were quantified by comparison to a calibration curve derived from the standard, rutin (0.215 mg/gm) quercetin (1.36 mg/gm), kaempferol (6.36 mg/gm) and luteolin (8.64 mg/gm).
  
  
  CONCLUSIONS
  The crude extract containing ethanolic extract of Givotia rottleriformis bark have potent antipsoriatic activity in UV-B-induced psoriasis in rat.
authors.affiliation: Department of Pharmacognosy, Sri Ramaswamy Memorial College of Pharmacy, Sri Ramaswamy Memorial University, Kattankulathur, Chengalpet, Tamil Nadu, India.
authors.forename: A
authors.initials: A
authors.lastname: Vijayalakshmi
authors.affiliation: Department of Pharmacognosy, Sri Ramaswamy Memorial College of Pharmacy, Sri Ramaswamy Memorial University, Kattankulathur, Chengalpet, Tamil Nadu, India.
authors.forename: Madhira
authors.initials: M
authors.lastname: Geetha
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D019665:Euphorbiaceae / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use* / Q000633:toxicity; D011565:Psoriasis / Q000188:drug therapy* / Q000276:immunology; D051381:Rats; D012867:Skin / Q000098:blood supply / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts
keywords: Anti-psoriatic; Givotia rottleriformis; UV-B photodermatits model; flavonoids
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