title: [Pregnane steroids from Nephthea sp. ].
abstract: OBJECTIVE
  To study the chemical constituents of Nephthea sp..
  
  
  METHODS
  The constituents were isolated and purified by silica gel, Sephadex LH-20 column chromatography as well as HPLC. Their structures were identified by spectroscopic analysis (NMR and MS) and their spectral data were compared with those of related steroids.
  
  
  RESULTS
  The compounds were isolated from the EtOAc fraction of Nephthea sp. 95% ethanol extract and identified as (17alpha)-pregnan-4-ene-3,20-dione(1), (20S)-pregnan-1,4-diene-3-oxo-20-carboxylic acid methyl ester(2), pregnan4-ene-3,6,20-trione(3), (20R)-pregnan-4,16-dien-20-hydroxy-3-one(4), pregnan-15beta-hydroxy4,6-diene-3,20-dione (5) and pregnan-4,6-diene-3, 20-dione (6), respectively.
  
  
  CONCLUSIONS
  All the compounds are isolated from Nephthea sp. for the first time.
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011278:Pregnanes / Q000737:chemistry* / Q000302:isolation & purification; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D011278:Pregnanes; D013256:Steroids; D000431:Ethanol