title: [Effect of Naringin on proliferation and osteogenic differentiation of bone marrow stromal cells in vitro].
abstract: OBJECTIVE
  To investigate the differentiation and osteogenic activity of Naringin-induced bone marrow stromal cells (BMSCs) in dogs.
  
  
  METHODS
  BMSCs were separated and cultured in vitro and identified by FCM. Then different concentration of Naringin (1×10⁻⁵, 1×10⁻⁶, 1×10⁻⁷, 1×10⁻⁸ and 1×10⁻⁹ mol/L) were added to cell culture media to induce BMSCs. The effect of Naringin on BMSCs was evaluated respectively by CCK-8 method and measuring the activity of alkaline phosphatase (ALP). The formation of nodules of calcium was detected by von Kossa staining. The data was analyzed with SPSS20.0 software package.
  
  
  RESULTS
  The result of cell-surface marker displayed that the expression of CD34 and CD45 were negative while the expression of CD90 was positive. The values were 0.126%, 0.075% and 95.4%, respectively. Naringin could obviously promote cell proliferation, which exhibited the best effect on proliferation and osteogenic differentiation at concentration of 10-6 mol/L. Calcium nodule (von Kossa) staining was positive.
  
  
  CONCLUSIONS
  Naringin-induced bone marrow stromal cells can be differentiated into osteoblasts. Naringin at the concentration of 10-6 mol/L can enhance the proliferation and osteogenic differentiation of BMSCs.
authors.affiliation: Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University. Shanghai 200072;China.E-mail:2011zzh@tongji.edu.cn.
authors.forename: Xue-bin
authors.initials: XB
authors.lastname: Zhang
authors.forename: Tian-lin
authors.initials: TL
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Ruan-jun
authors.initials: RJ
authors.lastname: Tan
authors.forename: Hai-liang
authors.initials: HL
authors.lastname: Ge
authors.forename: Yuan-liang
authors.initials: YL
authors.lastname: Huang
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004285:Dogs; D044950:Flavanones / Q000494:pharmacology*; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells; D010006:Osteoblasts; D010012:Osteogenesis
chemical_list: D044950:Flavanones; D000469:Alkaline Phosphatase; C005274:naringin