title: In vitro and in vivo evaluation of a novel collagen/cellulose nanocrystals scaffold for achieving the sustained release of basic fibroblast growth factor.
abstract: Tissue-engineered dermis is thought to be the best treatment for skin defects; however, slow vascularization of these biomaterial scaffolds limits their clinical application. Exogenous administration of angiogenic growth factors is highly desirable for tissue regeneration. In this study, biodegradable gelatin microspheres (GMs) containing basic fibroblast growth factor (bFGF) were fabricated and incorporated into a porous collagen/cellulose nanocrystals (CNCs) scaffold, as a platform for long-term release and consequent angiogenic boosting. The physicochemical properties of these scaffolds were examined and the in vitro release pattern of bFGF from scaffolds was measured by ELISA. Collagen/CNCs scaffolds with and without bFGF-GMs were incubated with human umbilical vein endothelial cells for 1 week, results showed that the scaffolds with bFGF-GMs significantly augmented cell proliferation. Then, four different groups of scaffolds were implanted subcutaneously into Sprague-Dawley rats to study angiogenesis in vivo via macroscopic observation, and hematoxylin and eosin and immunohistochemical staining. The results suggested that the collagen/CNCs/bFGF-GMs scaffolds had a significantly higher number of newly formed and mature blood vessels, and the fastest degradation rate. This study demonstrated that collagen/CNCs/bFGF-GMs scaffolds have great potential in skin tissue engineering.
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China Department of Chemistry, Jinan University, Guangzhou, China.
authors.forename: Weichang
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China Department of Biomedical Engineering, Jinan University, Guangzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lan
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China Department of Biomedical Engineering, Jinan University, Guangzhou, China guorui@jnu.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China Department of Biomedical Engineering, Jinan University, Guangzhou, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China Department of Biomedical Engineering, Jinan University, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
authors.affiliation: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, China Department of Chemistry, Jinan University, Guangzhou, China.
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D065966:Absorption, Physicochemical; D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage / Q000737:chemistry; D000818:Animals; D001808:Blood Vessels / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D002482:Cellulose / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000138:chemical synthesis*; D004058:Diffusion; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000737:chemistry; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry*; D018919:Neovascularization, Physiologic / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply; D017768:Skin, Artificial; D054457:Tissue Scaffolds
chemical_list: D043925:Angiogenesis Inducing Agents; D024042:Collagen Type I; D003692:Delayed-Action Preparations; D053769:Nanocapsules; D016222:Fibroblast Growth Factor 2; D002482:Cellulose
keywords: Collagen; angiogenesis; basic fibroblast growth factor; cellulose nanocrystals; gelatin microspheres; wound healing