title: Immunoprotection against influenza virus H9N2 by the oral administration of recombinant Lactobacillus plantarumNC8 expressing hemagglutinin in BALB/c mice.
abstract: The H9N2 avian influenza virus (AIV) has become increasingly concerning due to its role in severe economic losses in the poultry industry. Transmission of AIV to mammals, including pigs and humans, has accelerated efforts to devise preventive strategies. To develop an effective oral vaccine against H9N2 AIV, a recombinant Lactobacillus plantarum NC8 strain expressing the hemagglutinin (HA) gene of H9N2 AIV was constructed in this study. Mice were orally immunized with the recombinant NC8-pSIP409-HA strain, and sIgA, IgG and HI antibodies were produced by the NC8-pSIP409-HA strain, which also induced CD8(+) T cell immune responses. Most importantly, oral administration produced complete protection against challenge with mouse-adapted H9N2 virus. These results indicate that the recombinant NC8-pSIP409-HA was more effective at inducing the mucosal, humoral and cellular immune responses. Therefore, L. plantarum NC8-pSIP409-HA could become a promising oral vaccine candidate against H9N2 AIV.
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal probiotics, Changchun 130118, China; Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, 2888 Xincheng street, Changchun 130118, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal probiotics, Changchun 130118, China.
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal probiotics, Changchun 130118, China.
authors.forename: Gui-Lian
authors.initials: GL
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Cong
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal probiotics, Changchun 130118, China.
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal probiotics, Changchun 130118, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal probiotics, Changchun 130118, China.
authors.forename: Ruo-Peng
authors.initials: RP
authors.lastname: Cai
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal probiotics, Changchun 130118, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ye
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal probiotics, Changchun 130118, China.
authors.forename: Jing-Tao
authors.initials: JT
authors.lastname: Hu
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal probiotics, Changchun 130118, China.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal probiotics, Changchun 130118, China; Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, 2888 Xincheng street, Changchun 130118, China. Electronic address: wangchunfeng@jlau.edu.cn.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, 2888 Xincheng street, Changchun 130118, China. Electronic address: yuli966@126.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002645:Chickens; D005260:Female; D015870:Gene Expression; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D006801:Humans; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D014611:Vaccination
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines
keywords: H9N2 virus; Hemagglutinin; Oral vaccines; Recombinant L. Plantarum NC8-pSIP409-HA