title: Bioengineered functional brain-like cortical tissue.
abstract: The brain remains one of the most important but least understood tissues in our body, in part because of its complexity as well as the limitations associated with in vivo studies. Although simpler tissues have yielded to the emerging tools for in vitro 3D tissue cultures, functional brain-like tissues have not. We report the construction of complex functional 3D brain-like cortical tissue, maintained for months in vitro, formed from primary cortical neurons in modular 3D compartmentalized architectures with electrophysiological function. We show that, on injury, this brain-like tissue responds in vitro with biochemical and electrophysiological outcomes that mimic observations in vivo. This modular 3D brain-like tissue is capable of real-time nondestructive assessments, offering previously unidentified directions for studies of brain homeostasis and injury.
authors.affiliation: Department of Biomedical Engineering and.
authors.forename: Min D
authors.initials: MD
authors.lastname: Tang-Schomer
authors.affiliation: Department of Biomedical Engineering and.
authors.forename: James D
authors.initials: JD
authors.lastname: White
authors.affiliation: Department of Biomedical Engineering and.
authors.forename: Lee W
authors.initials: LW
authors.lastname: Tien
authors.affiliation: Department of Neuroscience, Tufts University Medical School, Boston, MA 02111.
authors.forename: L Ian
authors.initials: LI
authors.lastname: Schmitt
authors.affiliation: Department of Biomedical Engineering and.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Valentin
authors.affiliation: Department of Biomedical Engineering and.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Graziano
authors.affiliation: Department of Biomedical Engineering and.
authors.forename: Amy M
authors.initials: AM
authors.lastname: Hopkins
authors.affiliation: Department of Biomedical Engineering and Physics Department, Tufts University, Medford, MA 02155; and.
authors.forename: Fiorenzo G
authors.initials: FG
authors.lastname: Omenetto
authors.affiliation: Department of Neuroscience, Tufts University Medical School, Boston, MA 02111.
authors.forename: Philip G
authors.initials: PG
authors.lastname: Haydon
authors.affiliation: Department of Biomedical Engineering and david.kaplan@tufts.edu.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000628:therapy; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*
keywords: connectivity; electrophysiology; scaffold; silk; traumatic brain injury
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