title: Decreased P-glycoprotein is associated with the inhibitory effects of static magnetic fields and cisplatin on K562 cells.
abstract: In this study, we explored the mechanism of the killing effects of a moderate-intensity static magnetic field (SMF) and cisplatin (DDP) on K562 cells. We analyzed the metabolic activity of cells, the extracellular DDP content, and P-glycoprotein (P-gp) expression after K562 cells were exposed continuously to a uniform 8.8 mT SMF for 8 h, with or without DDP. We found that SMF combined with DDP (10 µg/ml) significantly inhibited the metabolic activity of K562 cells (P < 0.05), while neither DDP nor SMF alone affected the metabolic activity of these cells. In the SMF + DDP group, extracellular DDP content was significantly reduced (P < 0.05). DDP also induced the expression of P-gp (P < 0.05). By contrast, in the SMF + DDP group, P-gp expression decreased compared with the DDP group (P < 0.05). Taken together, our results showed that 8.8 mT SMF enhanced the killing potency of DDP on K562 cells by decreasing the expression of P-gp.
authors.affiliation: College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, P. R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Wenfang
authors.initials: W
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Bu
authors.forename: Hao
authors.initials: H
authors.lastname: Qi
authors.forename: Runguang
authors.initials: R
authors.lastname: Sun
authors.forename: Xiao
authors.initials: X
authors.lastname: He
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000494:pharmacology*; D005110:Extracellular Space / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D020014:K562 Cells; D060526:Magnetic Fields; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D011838:Radiation-Sensitizing Agents; D002945:Cisplatin
keywords: K562 cell; P-glycoprotein, cisplatin; metabolic activity; static magnetic field