title: Organochlorinated pesticide degrading microorganisms isolated from contaminated soil.
abstract: Degradation of selected organochlorinated pesticides (γ-hexachlorocyclohexane - γ-HCH, dichlorodiphenyltrichloroethane - DDT, hexachlorobenzene - HCB) by soil microorganisms was studied. Bacterial strains isolated from contaminated soil from Klatovy-Luby, Hajek and Neratovice, Czech Republic, capable of growth on the selected pesticides were isolated and characterised. These isolates were subjected to characterisation and identification by MS MALDI-TOF of whole cells and sequence analysis of 16S rRNA genes. The isolates were screened by gas chromatography for their ability to degrade the selected pesticides. Some isolates were able to degrade pesticides, and the formation of degradation products (γ-pentachlorocyclohexane (γ-PCCH), dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DDD)) observed in liquid culture confirmed their degradation capability. The isolates and DNA samples isolated from the contaminated soil were also screened for the bphA1 gene (encoding biphenyl-2,3-dioxygenase, the first enzyme in the PCB degradation pathway) and its occurrence was demonstrated. The isolates were also screened for the presence of linA, encoding dehydrochlorinase, the first enzyme of the HCH degradation pathway. The linA gene could not be found in any of the tested isolates, possibly due to the high specificity of the primers used. The isolates with the most effective degradation abilities could be used for further in situ bioremediation experiments with contaminated soil.
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 3, Prague 166 28, Czech Republic. Electronic address: loveckap@vscht.cz.
authors.forename: Petra
authors.initials: P
authors.lastname: Lovecka
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 3, Prague 166 28, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Pacovska
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 3, Prague 166 28, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Stursa
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 3, Prague 166 28, Czech Republic.
authors.forename: Blanka
authors.initials: B
authors.lastname: Vrchotova
authors.affiliation: Department of Environmental Chemistry, Institute of Chemical Technology, Technicka 3, Prague 166 28, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Kochankova
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 3, Prague 166 28, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Demnerova
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D001673:Biodegradation, Environmental / Q000187:drug effects; D002849:Chromatography, Gas; D004269:DNA, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D050296:Microbial Viability / Q000187:drug effects; D010575:Pesticides / Q000378:metabolism* / Q000633:toxicity; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004269:DNA, Bacterial; D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants