title: TRIM72 is required for effective repair of alveolar epithelial cell wounding.
abstract: The molecular mechanisms for lung cell repair are largely unknown. Previous studies identified tripartite motif protein 72 (TRIM72) from striated muscle and linked its function to tissue repair. In this study, we characterized TRIM72 expression in lung tissues and investigated the role of TRIM72 in repair of alveolar epithelial cells. In vivo injury of lung cells was introduced by high tidal volume ventilation, and repair-defective cells were labeled with postinjury administration of propidium iodide. Primary alveolar epithelial cells were isolated and membrane wounding and repair were labeled separately. Our results show that absence of TRIM72 increases susceptibility to deformation-induced lung injury whereas TRIM72 overexpression is protective. In vitro cell wounding assay revealed that TRIM72 protects alveolar epithelial cells through promoting repair rather than increasing resistance to injury. The repair function of TRIM72 in lung cells is further linked to caveolin 1. These data suggest an essential role for TRIM72 in repair of alveolar epithelial cells under plasma membrane stress failure.
authors.affiliation: Division of Pharmacology, College of Pharmacy, The Ohio State University, Columbus, Ohio;
authors.forename: Seong Chul
authors.initials: SC
authors.lastname: Kim
authors.affiliation: Division of Pharmacology, College of Pharmacy, The Ohio State University, Columbus, Ohio;
authors.forename: Thomas
authors.initials: T
authors.lastname: Kellett
authors.affiliation: Thoracic Diseases Research Unit, Mayo Clinic, Rochester, Minnesota;
authors.forename: Shaohua
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Biological Chemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Kyoto, Japan;
authors.forename: Miyuki
authors.initials: M
authors.lastname: Nishi
authors.affiliation: Division of Pharmacology, College of Pharmacy, The Ohio State University, Columbus, Ohio;
authors.forename: Nagaraja
authors.initials: N
authors.lastname: Nagre
authors.affiliation: Will Rogers Institute Pulmonary Research Center, Division of Pulmonary, Critical Care and Sleep Medicine, University of Southern California, Los Angeles, California;
authors.forename: Beiyun
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Will Rogers Institute Pulmonary Research Center, Division of Pulmonary, Critical Care and Sleep Medicine, University of Southern California, Los Angeles, California;
authors.forename: Per
authors.initials: P
authors.lastname: Flodby
authors.affiliation: Thoracic Pathology Division, Department of Pathology, The Ohio State University, Columbus, Ohio;
authors.forename: Konstantin
authors.initials: K
authors.lastname: Shilo
authors.affiliation: Biomedical Engineering Department, College of Engineering, The Ohio State University, Columbus, Ohio; and.
authors.forename: Samir N
authors.initials: SN
authors.lastname: Ghadiali
authors.affiliation: Department of Biological Chemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Kyoto, Japan;
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takeshima
authors.affiliation: Thoracic Diseases Research Unit, Mayo Clinic, Rochester, Minnesota;
authors.forename: Rolf D
authors.initials: RD
authors.lastname: Hubmayr
authors.affiliation: Division of Pharmacology, College of Pharmacy, The Ohio State University, Columbus, Ohio; Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, College of Medicine, The Ohio State University, Columbus, Ohio zhao.1028@osu.edu.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins; D051379:Mice; D018345:Mice, Knockout; D011650:Pulmonary Alveoli / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing
chemical_list: D002352:Carrier Proteins; C495541:Cav1 protein, mouse; D051242:Caveolin 1; C538824:MG53 protein, mouse; D008565:Membrane Proteins
keywords: acute lung injury; alveolar epithelial cells; caveolin 1; plasma membrane wounding and repair; tripartite motif protein 72
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