title: HMGA1 is a new target of miR-195 involving isoprenaline-induced cardiomyocyte hypertrophy.
abstract: Emerging data have shown that microRNAs (miRNAs) have important functions in the processes of cardiac hypertrophy and heart failure that occur during the postnatal period. Cardiac overexpression of miR-195 results in pathological cardiac growth and heart failure in transgenic mice. In the present study, we analyzed the roles of miR-195 in cardiomyocyte hypertrophy and found that miR-195 was greatly upregulated during isoprenaline-induced cardiomyocyte hypertrophy. By using mRNA microarray and molecular approach, we identified a novel putative target of miR-195 called high-mobility group A1 (HMGA1). Total mRNA microarray showed that HMGA1 was downregulated in primary cardiomyocytes that overexpressed miR-195. Using luciferase activity assay, we demonstrated that miR-195 interacts with the 3'-untranslated region of HMGA1 mRNA. Moreover, we showed that miR-195 in primary cardiomyocytes downregulates the expression of HMGA1 at the protein level. Taken together, our data demonstrated that miR-195 can negatively regulate a new target, HMGA1, which is involved in cardiomyocyte hypertrophy.
authors.affiliation: Cardiovascular Department, Second Hospital Affiliated to Guangzhou Medical University, Guangzhou Institute of Cardiovascular Disease, Guangzhou, 510260, PR China. gzliushiming@126.com.
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: You
authors.forename: Jiong-Hua
authors.initials: JH
authors.lastname: Huang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Shao-Jun
authors.initials: SJ
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhong
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D006332:Cardiomegaly / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D015536:Down-Regulation; D025741:HMGA1a Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D007545:Isoproterenol / Q000633:toxicity; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; C569389:MIRN195a microRNA, mouse; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D025741:HMGA1a Protein; D007545:Isoproterenol