title: Production of surfactin using pentose carbohydrate by Bacillus subtilis.
abstract: Interest in microbial surfactants has been steadily increasing in recent years due to their diversity, mass production possibility, selectivity, performance under extreme conditions and potential applications in environmental protection. In this study two pentose sugars (xylose and arabinose) were investigated for the submerged fermentation (SmF) of Bacillus subtilis in surfactant production medium for bio-surfactant surfactin production. An excellent vegetative growth of B. subtilis (× 10(10) CFU/mL) was observed for xylose and arabinose containing medium which were comparable to glucose supplemented medium. Low growth (× 10(8) CFU/mL) was found when medium was not supplemented with any of the sugars. Surfactin production in xylose, arabinose and glucose containing medium was 2700, 2600 and 2000 mg/L, respectively, whereas, medium without any sugar showed low surfactin (700 mg/L) production. These results clearly indicate the effect of pentose sugars on production of surfactin. Gradual depletion of the xylose and arabinose were confirmed by HPLC analysis during the growth phase of the strain that ultimately produced the surfactin.
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; Institute of Biological Resources, Anise Corporation, N605 Building #C, 1-12 Minamiwatarida-cho, Kawasaki-ku, Kawasaki-shi 210-0855, Japan.
authors.forename: Abdul Wahab
authors.initials: AW
authors.lastname: Khan
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; Institute of Biological Resources, Anise Corporation, N605 Building #C, 1-12 Minamiwatarida-cho, Kawasaki-ku, Kawasaki-shi 210-0855, Japan.
authors.forename: Mohammad Shahedur
authors.initials: MS
authors.lastname: Rahman
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; Institute of Biological Resources, Anise Corporation, N605 Building #C, 1-12 Minamiwatarida-cho, Kawasaki-ku, Kawasaki-shi 210-0855, Japan.
authors.forename: Umme Salma
authors.initials: US
authors.lastname: Zohora
authors.affiliation: Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa-city, Wakayama, 649-6493, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okanami
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa-city, Wakayama, 649-6493, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ano
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D010429:Pentoses / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D010429:Pentoses; D013501:Surface-Active Agents; D002244:Carbon