title: Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite.
abstract: A computationally engineered impedimetric naltrexone (NLT) biosensor based on immobilization of bovine serum albumin (BSA) onto fullerene-C60/glassy carbon electrode (FLR/GCE) has been developed using initial characterization by computational methods and complementing them by experimental ones. Computational results showed that BSA hydrophobically binds to FLR which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite and also showed that interaction of NLT with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. Besides complementing the computational studies, experimental results showed that addition of FLR to the surface of the electrode facilitated electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the non-conductive properties of BSA. The presence of NLT may form a negatively charged electroactive complex with BSA which repels the negatively charged redox probe and decelerates interfacial electron transfer leading to obvious faradaic impedance change. The faradaic impedance responses were linearly related to naltrexone concentration between 0.1 nM and 80 nM and limit of detection (LOD) was calculated to be 0.01 nM 3Sb/b. Finally, the proposed biosensor was successfully applied to determination of NLT in urine samples of both healthy and addict volunteers.
authors.affiliation: Faculty of Chemistry, Razi University, Kermanshah 671496734, Iran. Electronic address: mbgholivand2013@gmail.com.
authors.forename: Mohammad-Bagher
authors.initials: MB
authors.lastname: Gholivand
authors.affiliation: Faculty of Chemistry, Razi University, Kermanshah 671496734, Iran; Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Universidad Nacional del Litoral, Ciudad Universitaria, CC 242, S3000ZAA Santa Fe, Argentina.
authors.forename: Ali R
authors.initials: AR
authors.lastname: Jalalvand
authors.affiliation: Faculty of Chemistry, Razi University, Kermanshah 671496734, Iran.
authors.forename: Giti
authors.initials: G
authors.lastname: Paimard
authors.affiliation: Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Universidad Nacional del Litoral, Ciudad Universitaria, CC 242, S3000ZAA Santa Fe, Argentina.
authors.forename: Hector C
authors.initials: HC
authors.lastname: Goicoechea
authors.affiliation: Quality & Technology, Department of Food Science, Faculty of Science, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Thomas
authors.initials: T
authors.lastname: Skov
authors.affiliation: Faculty of Chemistry, Razi University, Kermanshah 671496734, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Farhadi
authors.affiliation: Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
authors.forename: Sirous
authors.initials: S
authors.lastname: Ghobadi
authors.affiliation: Faculty of Chemistry, Razi University, Kermanshah 671496734, Iran.
authors.forename: Nozar
authors.initials: N
authors.lastname: Moradi
authors.affiliation: Faculty of Chemistry, Razi University, Kermanshah 671496734, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Nasirian
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D003198:Computer Simulation; D004563:Electrochemistry; D004566:Electrodes; D009271:Naltrexone / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D001672:Biocompatible Materials; D009271:Naltrexone
keywords: Biosensor; Bovine serum albumin; Computational and experimental characterizations; Fullerene-C(60); Naltrexone