title: Suitable technological conditions for enzymatic hydrolysis of waste paper by Novozymes® enzymes NS50013 and NS50010.
abstract: Waste paper belongs to a group of quantitatively the most produced waste types. Enzymatic hydrolysis is becoming a suitable way to treat this type of waste and at the same time, to produce a valuable liquid biofuel, because reducing sugars solutions that are formed during the process of saccharification can be a precursor for following or simultaneous fermentation. If it will be possible to make the enzymatic hydrolysis of the waste paper economically viable, it could serve as one of the new ways to lower the dependence of the transport sector on oil in the future. Only several studies comparing the enzymatic hydrolysis of different waste papers were performed in the past; they are summarized in this manuscript. In our experimental trials, suitable technological conditions for waste paper enzymatic hydrolysis using enzymes from Novozymes® biomass kit: enzymes NS50013 and NS50010 were investigated. The following enzymatic hydrolysis parameters in laboratory scale trials were verified on high cellulose content substrates-filter paper and cellulose pulp: type of buffer, pH, temperature, concentration of the substrate, loading of the enzyme and rate of stirring.
authors.affiliation: Institute of Process and Environmental Engineering, Brno University of Technology, Technicka 2896/2, 61669, Brno, Czech Republic. brummer@fme.vutbr.cz.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Brummer
authors.affiliation: Institute of Process and Environmental Engineering, Brno University of Technology, Technicka 2896/2, 61669, Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Skryja
authors.affiliation: Institute of Process and Environmental Engineering, Brno University of Technology, Technicka 2896/2, 61669, Brno, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Jurena
authors.affiliation: Institute of Food Science and Biotechnology, Brno University of Technology, Purkynova 118, 61200, Brno, Czech Republic.
authors.forename: Viliam
authors.initials: V
authors.lastname: Hlavacek
authors.affiliation: Institute of Process and Environmental Engineering, Brno University of Technology, Technicka 2896/2, 61669, Brno, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Stehlik
mesh_terms: D044602:Cellulases / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D006868:Hydrolysis; D010209:Paper; D012037:Refuse Disposal / Q000379:methods*
chemical_list: D002482:Cellulose; D044602:Cellulases