title: CXCR7 Receptor Controls the Maintenance of Subpial Positioning of Cajal-Retzius Cells.
abstract: Cajal-Retzius (CR) cells are essential for cortical development and lamination. These pioneer neurons arise from distinct progenitor sources, including the cortical hem and the ventral pallium at pallium-subpallium boundary (PSB). CXCR4, the canonical receptor for the chemokine CXCL12, controls the superficial location of hem-derived CR cells. However, recent studies showed that CXCR7, a second CXCL12 receptor, is also expressed in CR cells at early developmental stages. We thus investigated the role of CXCR7 during CR cell development using multiple loss-of-function approaches. Cxcr7 gene inactivation led to aberrant localization of Reelin-positive cells within the pallium. In addition, Cxcr7(-/-) mice were characterized by significant accumulation of ectopic CR cells in the lateral part of the dorsal pallium compared with Cxcr4 knockout mice. Loss-of-function approaches, using either gene targeting or pharmacological receptor inhibition, reveal that CXCR7 and CXCR4 act both in CR positioning. Finally, conditional Cxcr7 deletion in cells derived from Dbx1-expressing progenitors indicates an essential role of CXCR7 in controlling the positioning of a subpopulation of PSB-derived CR cells. Our data demonstrate that CXCR7 has a role in the positioning of hem and PSB-derived CR cells, CXCL12 regulating CR cell subpial localization through the combined action of CXCR4 and CXCR7.
authors.affiliation: Université Montpellier 2, Montpellier F-34095, France INSERM U710, University Montpellier 2, Montpellier F-34095, France EPHE, Paris F-75007, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Trousse
authors.affiliation: Anatomy, Physiology and Genetics Neuroscience, Uniformed Services University, Bethesda, MD, USA.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Poluch
authors.affiliation: CNRS UMR 7592, Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pierani
authors.affiliation: CNRS UMR 7592, Institut Jacques Monod, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Dutriaux
authors.affiliation: Gastroenterology, Hepatology and Infectiology Department, University Hospital Düsseldorf, 40225 Düsseldorf, Germany.
authors.forename: Hans H
authors.initials: HH
authors.lastname: Bock
authors.affiliation: Department of Immunobiology and Hematology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagasawa
authors.affiliation: Université Montpellier 2, Montpellier F-34095, France INSERM U710, University Montpellier 2, Montpellier F-34095, France EPHE, Paris F-75007, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Verdier
authors.affiliation: Université Montpellier 2, Montpellier F-34095, France INSERM U710, University Montpellier 2, Montpellier F-34095, France EPHE, Paris F-75007, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Rossel
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D002465:Cell Movement; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D054387:Receptors, CXCR / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C514936:CXCR4 protein, mouse; D015816:Cell Adhesion Molecules, Neuronal; C501897:Cmkor1 protein, mouse; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D054387:Receptors, CXCR; D019718:Receptors, CXCR4; D000091042:Reelin Protein; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases
keywords: Dbx1; Reelin; chemokine receptor; mouse; neocortical development; pallium–subpallium boundary