title: Global transcriptome profiling of genes that are differentially regulated during differentiation of mouse embryonic neural stem cells into astrocytes.
abstract: Many genes are associated with the differentiation of neural stem cells (NSCs) into astrocytes, the most abundant and functionally diverse population of glial cells in the central nervous system, particularly in the brain. In the present study, we differentiated NSCs from the forebrain of embryonic day 14.5 mouse embryos into astrocytes over 1 and 7 days. We identified transcriptomes of NSCs and astrocytes using RNA sequencing and analyzed enriched gene networks, signal pathways, and ontology. To identify important regulators of differentiation, we performed gene clustering according to expression patterns and promoter CG types. Our data show that genes related to system development, including Fbln2, Bcan, Ncam1, Itih3, Tnr, and Vcan, regulate NSC differentiation through WNT/beta-catenin and epithelial to mesenchymal transition pathways. We identified many CG-rich promoter genes related to basic cellular maintenance such as transcription, translation, and structural components and CG-poor promoter genes that are highly associated with cell-type-specific functions or play important roles during development. Our study provides a foundation for further research on NSC differentiation and the future application of stem cells.
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, 426-791, Korea.
authors.forename: Dalmuri
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, 426-791, Korea.
authors.forename: Mi Ran
authors.initials: MR
authors.lastname: Choi
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, 426-791, Korea.
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Jung
authors.affiliation: Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea.
authors.forename: Namshin
authors.initials: N
authors.lastname: Kim
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, 426-791, Korea.
authors.forename: Se Kye
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, 426-791, Korea.
authors.forename: Jin Choul
authors.initials: JC
authors.lastname: Chai
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, 426-791, Korea.
authors.forename: Young Seek
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, 426-791, Korea. ygchai@hanyang.ac.kr.|Department of Nanobiotechnology, Hanyang University, Seoul, 131-791, Korea. ygchai@hanyang.ac.kr.
authors.forename: Young Gyu
authors.initials: YG
authors.lastname: Chai
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D055495:Neurogenesis; D059467:Transcriptome; D060449:Wnt Signaling Pathway
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