title: Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats.
abstract: BACKGROUND
  The GABAergic system plays an important role in modulating levels of anxiety. When transplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) have the ability to differentiate into GABAergic interneurons and modify the inhibitory tone in the host brain. Currently, two methods have been reported for obtaining MGE precursor cells for transplantation: fresh and neurosphere dissociated cells. Here, we investigated the effects generated by transplantation of the two types of cell preparations on anxiety behavior in rats.
  
  
  RESULTS
  We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain of male rats on postnatal (PN) day 2-3. At early adulthood (PN 62-63), transplanted animals were tested in the Elevated Plus Maze (EPM). To verify the differentiation and migration pattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry for GFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) and calretinin (CR). Cells from both types of preparations expressed these interneuronal markers. However, an anxiolytic effect on behavior in the EPM was observed in animals that received the MGE-derived freshly dissociated cells but not in those that received the neurosphere dissociated cells.
  
  
  CONCLUSIONS
  Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cells that reinforces the inhibitory function of the GABAergic neuronal circuitry in the hippocampus related to anxiety-like behavior in rats.
authors.forename: Simone Amaro Alves
authors.initials: SA
authors.lastname: Romariz
authors.forename: Daisyléa de Souza
authors.initials: Dde S
authors.lastname: Paiva
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Valente
authors.forename: Gabriela Filoso
authors.initials: GF
authors.lastname: Barnabé
authors.forename: Roberto
authors.initials: R
authors.lastname: Frussa-Filho
authors.forename: Regina Cláudia
authors.initials: RC
authors.lastname: Barbosa-Silva
authors.forename: Maria Elisa
authors.initials: ME
authors.lastname: Calcagnotto
authors.affiliation: Departamento de Fisiologia, UNIFESP, Rua Botucatu, 862, 5° andar, 04023-062 São Paulo, SP, Brazil. beatrizlongo@gmail.com.
authors.forename: Beatriz Monteiro
authors.initials: BM
authors.lastname: Longo
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001007:Anxiety / Q000503:physiopathology / Q000628:therapy*; D064032:Calbindin 2 / Q000378:metabolism; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000502:physiology; D017690:Cell Transplantation / Q000379:methods*; D002540:Cerebral Cortex / Q000503:physiopathology; D053595:Embryonic Stem Cells / Q000502:physiology / Q000637:transplantation*; D005106:Exploratory Behavior / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000503:physiopathology; D007395:Interneurons / Q000502:physiology; D008297:Male; D008473:Median Eminence / Q000196:embryology / Q000502:physiology / Q000637:transplantation*; D058953:Neural Stem Cells / Q000502:physiology / Q000637:transplantation*; D055495:Neurogenesis / Q000502:physiology; D009478:Neuropeptide Y / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic
chemical_list: C576512:Calb2 protein, rat; D064032:Calbindin 2; D009478:Neuropeptide Y; D010320:Parvalbumins; D049452:Green Fluorescent Proteins
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