title: Combination of acellular nerve graft and schwann cells-like cells for rat sciatic nerve regeneration.
abstract: OBJECTIVE
  To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.
  
  
  METHODS
  Forty-five rats with defective sciatic nerve were randomly divided into three groups. Rats in group A were repaired by acellular nerve grafts only. Rats in group B were repaired by tissue engineering nerve. In group C, rats were repaired by autogenous nerve grafts. After six and twelve weeks, sciatic nerve functional index (SFI), neural electrophysiology (NEP), histological and transmission electron microscope observation, recovery ratio of wet weight of gastrocnemius muscle, regenerated myelinated nerve fibers number, nerve fiber diameter, and thickness of the myelin sheath were measured to assess the effect.
  
  
  RESULTS
  After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).
  
  
  CONCLUSIONS
  The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.
authors.affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.
authors.forename: Songtao
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe Road, Zhengzhou, Henan 450052, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.
authors.forename: Qiqing
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Department of Orthopedics, Xiangya Hospital of Central South University, No. 87, Xiangya Road, Changsha, Hunan 410008, China.
authors.forename: Zhansheng
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.
authors.forename: Weitao
authors.initials: W
authors.lastname: Yao
authors.affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000818:Animals; D009186:Myelin Sheath / Q000648:ultrastructure; D009416:Nerve Regeneration; D009417:Nerve Tissue / Q000637:transplantation*; D059348:Peripheral Nerve Injuries / Q000601:surgery*; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000502:physiology / Q000637:transplantation* / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000293:injuries* / Q000502:physiology* / Q000648:ultrastructure; D023822:Tissue Engineering
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