title: [Study on characteristic HPLC fingerprint of Polygonum orientale inflorescence].
abstract: OBJECTIVE
  To establish a characteristic HPLC fingerprint of Polygonum orientale inflorescence, and to provide reference for its quality evaluation.
  
  
  METHODS
  Taxifolin was used as reference. HPLC analysis was carried out with Diamonsil C18 column (200 mm x 4.6 mm, 5 microm) using acetonitrile -0.1% phosphoric acid(gradient elution)as mobile phase at flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm and the column temperature was 30 degrees C.
  
  
  RESULTS
  Eighteen common peaks were pointed out from the HPLC fingerprint of Polygonum orientale inflorescence from 12 different habitats. Among of them,four common peaks were identified as taxifolin, catechin, gallic acid and 3,3'-dimethyl ellagic acid-4-O-beta-D-glucoside. Analyzed by "Similarity Evaluation for Chromatographic Fingerprint of Traditional Chinese Medicine" software, the HPLC fingerprint similarities of 12 samples were more than 0.9.
  
  
  CONCLUSIONS
  This method is repeatable and exclusive. It can be used for identification and quality control of Polygonum orientale inflorescence.
authors.forename: Yu-yao
authors.initials: YY
authors.lastname: Zhang
authors.forename: Miao-miao
authors.initials: MM
authors.lastname: Tong
authors.forename: Zheng-yun
authors.initials: ZY
authors.lastname: Chu
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Kang
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Zhai
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D011794:Quercetin; C003377:taxifolin