title: The proteome of Atlantic herring (Clupea harengus L.) larvae is resistant to elevated pCO2.
abstract: Elevated anthropogenic pCO2 can delay growth and impair otolith structure and function in the larvae of some fishes. These effects may concurrently alter the larva's proteome expression pattern. To test this hypothesis, Atlantic herring larvae were exposed to ambient (370 μatm) and elevated (1800 μatm) pCO2 for one-month. The proteome structure of the larvae was examined using a 2-DE and mass spectrometry. The length of herring larvae was marginally less in the elevated pCO2 treatment compared to the control. The proteome structure was also different between the control and treatment, but only slightly: the expression of a small number of proteins was altered by a factor of less than 2-fold at elevated pCO2. This comparative proteome analysis suggests that the proteome of herring larvae is resilient to elevated pCO2. These observations suggest that herring larvae can cope with levels of CO2 projected for near future without significant proteome-wide changes.
authors.affiliation: Center for Environment and Water, Research Institute, King Fahd University of Petroleum and Minerals, 31261 Dhahran, Saudi Arabia; Helmholtz-Zentrum für Ozeanforschung Kiel-GEOMAR, Düsternbrooker Weg 20, 24105 Kiel, Germany.
authors.forename: Rommel H
authors.initials: RH
authors.lastname: Maneja
authors.affiliation: The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region.
authors.forename: R
authors.initials: R
authors.lastname: Dineshram
authors.affiliation: The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region. Electronic address: rajan@hku.hk.
authors.forename: Vengatesen
authors.initials: V
authors.lastname: Thiyagarajan
authors.affiliation: Institute of Marine Research, Austevoll Research Station, 5392 Storebø, Norway.
authors.forename: Anne Berit
authors.initials: AB
authors.lastname: Skiftesvik
authors.affiliation: Helmholtz-Zentrum für Ozeanforschung Kiel-GEOMAR, Düsternbrooker Weg 20, 24105 Kiel, Germany.
authors.forename: Andrea Y
authors.initials: AY
authors.lastname: Frommel
authors.affiliation: Helmholtz-Zentrum für Ozeanforschung Kiel-GEOMAR, Düsternbrooker Weg 20, 24105 Kiel, Germany.
authors.forename: Catriona
authors.initials: C
authors.lastname: Clemmesen
authors.affiliation: Department of Biology, University of Bergen, PO Box 7803, N-5020 Bergen, Norway.
authors.forename: Audrey J
authors.initials: AJ
authors.lastname: Geffen
authors.affiliation: Institute of Marine Research, Austevoll Research Station, 5392 Storebø, Norway. Electronic address: howard.browman@imr.no.
authors.forename: Howard I
authors.initials: HI
authors.lastname: Browman
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology*; D057231:Climate Change; D005399:Fishes / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D007814:Larva / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010037:Otolithic Membrane / Q000187:drug effects*; D020543:Proteome / Q000378:metabolism*
chemical_list: D020543:Proteome; D002245:Carbon Dioxide
keywords: Atlantic herring; Clupea harengus; Environmental proteomics; Fish larvae; Ocean acidification