title: Physiological conditioning by electric field stimulation promotes cardiomyogenic gene expression in human cardiomyocyte progenitor cells.
abstract: The optimal cell lineage for cardiac-regeneration approaches remains mysterious. Additionally, electrical stimulation promotes cardiomyogenic differentiation of stimulated cells. Therefore, we hypothesized that electrical conditioning of cardiomyocyte progenitor cells (CMPCs) might enrich their cardiovascular potential. CMPCs were isolated from human adult atrial appendages, characterized, and electrically stimulated for 7 and 14 days. Electrical stimulation modulated CMPCs gene and protein expression, increasing all cardiac markers. GATA-binding protein 4 (GATA4) early transcription factor was significantly overexpressed (P = 0.008), but also its coactivator myocyte enhancer factor 2A (MEF2A) was upregulated (P = 0.073) under electrical stimulation. Moreover, important structural proteins and calcium handling-related genes were enhanced. The cardioregeneration capability of CMPCs is improved by electrical field stimulation. Consequently, short-term electrical stimulation should be a valid biophysical approach to modify cardiac progenitor cells toward a cardiogenic phenotype, and can be incorporated into transdifferentiation protocols. Electrostimulated CMPCs may be best-equipped cells for myocardial integration after implantation.
authors.forename: Aida
authors.initials: A
authors.lastname: Llucià-Valldeperas
authors.forename: Benjamin
authors.initials: B
authors.lastname: Sanchez
authors.forename: Carolina
authors.initials: C
authors.lastname: Soler-Botija
authors.forename: Carolina
authors.initials: C
authors.lastname: Gálvez-Montón
authors.forename: Santiago
authors.initials: S
authors.lastname: Roura
authors.forename: Cristina
authors.initials: C
authors.lastname: Prat-Vidal
authors.forename: Isaac
authors.initials: I
authors.lastname: Perea-Gil
authors.forename: Javier
authors.initials: J
authors.lastname: Rosell-Ferrer
authors.forename: Ramon
authors.initials: R
authors.lastname: Bragos
authors.forename: Antoni
authors.initials: A
authors.lastname: Bayes-Genis
mesh_terms: D018929:Cell Culture Techniques; D004558:Electric Stimulation; D015870:Gene Expression; D006801:Humans; D024510:Muscle Development / Q000235:genetics; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
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