title: Production of 1,5-anhydro-d-fructose by an α-glucosidase belonging to glycoside hydrolase family 31.
abstract: α-1,4-Glucan lyases [glycoside hydrolase family (GH) 31] catalyze an elimination reaction to form 1,5-anhydro-d-fructose (AF), while GH31 α-glucosidases normally catalyze a hydrolytic reaction. We determined that a small amount of AF was produced by GH31 Aspergillus niger α-glucosidase from maltooligosaccharides by elimination reaction, likely via an oxocarbenium ion intermediate.
authors.affiliation: a Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors.forename: Janjira
authors.initials: J
authors.lastname: Maneesan
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsuura
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Tagami
authors.forename: Haruhide
authors.initials: H
authors.lastname: Mori
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kimura
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry* / Q000201:enzymology; D055162:Biocatalysis; D002236:Carbohydrate Conformation; D002247:Carbon Isotopes; D005632:Fructose / Q000031:analogs & derivatives* / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D007700:Kinetics; D009844:Oligosaccharides / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D002247:Carbon Isotopes; D005656:Fungal Proteins; D009844:Oligosaccharides; C021705:maltooligosaccharides; D005632:Fructose; C048927:1,5-anhydrofructose; D000520:alpha-Glucosidases
keywords: 1,5-anhydro-d-fructose; GH31 α-glucosidase; elimination mechanism