title: High-grain diets suppress ruminal tissue abundance of angiopoietin-like protein 4 in cattle.
abstract: Angiopoietin-like protein 4 (ANGPTL4) is expressed in bovine ruminal epithelium, making it possible that dietary components or commensal microbes may influence gastrointestinal ANGPTL4 production via interactions at the mucosal surface. Therefore, we conducted 3 experiments to evaluate the effects of dietary concentrate level and VFA infusions on ANGPTL4 abundance in ruminal tissue. In Exp. 1, we assigned 12 nonlactating Holstein cows to either 8% or 64% concentrate diets; diets were fed 28 d before euthanasia and ruminal tissue collection. Ruminal tissue and plasma ANGPTL4 protein abundance were unaltered by treatment. In Exp. 2, we assigned 8 continental crossbred heifers to either a 45% or 90% concentrate diet; diets were fed for 75 d before euthanasia and ruminal tissue collection. Compared with the 45% concentrate diet, the 90% concentrate diet decreased (P < 0.01) ruminal tissue ANGPTL4 protein abundance. In Exp. 3, we assigned 6 ruminally cannulated lactating Holstein cows to a treatment sequence in replicated 3 × 3 Latin squares and fed a standard lactation diet. Cows were infused with 5 mol/d sodium acetate, sodium propionate, or sodium butyrate for 2 d. Infusions of VFA did not affect (P > 0.10) mRNA or protein abundance of ANGPTL4 in ruminal papillae. Ruminal papillae ANGPTL4 abundance was, however, negatively correlated with (P = 0.01) ruminal VFA concentrations. ANGPTL4 abundance in ruminal tissue decreases in response to very high dietary concentrate and is inversely correlated with ruminal total VFA concentrations.
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: K
authors.initials: K
authors.lastname: Yuan
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: L K
authors.initials: LK
authors.lastname: Mamedova
authors.affiliation: Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon S7N 5A8, Canada.
authors.forename: G B
authors.initials: GB
authors.lastname: Penner
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton T6G 2P5, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Oba
authors.affiliation: Agriculture and Agri-Food Canada, Research Center, Lethbridge T1J 4B1, Canada.
authors.forename: K A
authors.initials: KA
authors.lastname: Beauchemin
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506 bbradfor@ksu.edu.
authors.forename: B J
authors.initials: BJ
authors.lastname: Bradford
mesh_terms: D042682:Angiopoietins / Q000378:metabolism*; D000818:Animals; D020148:Butyric Acid / Q000494:pharmacology; D002417:Cattle / Q000378:metabolism*; D004032:Diet / Q000662:veterinary*; D002523:Edible Grain / Q000378:metabolism*; D005260:Female; D007774:Lactation / Q000502:physiology; D011422:Propionates / Q000494:pharmacology; D012417:Rumen / Q000378:metabolism*; D019346:Sodium Acetate / Q000494:pharmacology
chemical_list: D042682:Angiopoietins; D011422:Propionates; D020148:Butyric Acid; D019346:Sodium Acetate; C514135:sodium propionate
keywords: angiopoietin-like protein 4; cattle; volatile fatty acid