title: Urinary CD133+ extracellular vesicles are decreased in kidney transplanted patients with slow graft function and vascular damage.
abstract: Extracellular vesicles (EVs) present in the urine are mainly released from cells of the nephron and can therefore provide information on kidney function. We here evaluated the presence of vesicles expressing the progenitor marker CD133 in the urine of normal subjects and of patients undergoing renal transplant. We found that EV expressing CD133 were present in the urine of normal subjects, but not of patients with end stage renal disease. The first day after transplant, urinary CD133+ EVs were present at low levels, to increase thereafter (at day 7). Urinary CD133(+) EVs significantly increased in patients with slow graft function in respect to those with early graft function. In patients with a severe pre-transplant vascular damage of the graft, CD133(+) EVs did not increase at day 7. At variance, the levels of EVs expressing the renal exosomal marker CD24 did not vary in the urine of patients with end stage renal disease or in transplanted patients in respect to controls. Sorted CD133(+) EVs were found to express glomerular and proximal tubular markers. These data indicate that urinary CD133(+) EVs are continuously released during the homeostatic turnover of the nephron and may provide information on its function or regenerative potential.
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy.
authors.forename: Veronica
authors.initials: V
authors.lastname: Dimuccio
authors.affiliation: Department of Medical Sciences, University of Torino, Torino, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ranghino
authors.affiliation: Immunogenetics and Transplant Biology Service, Città della Salute e della Scienza Hospital, Torino, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Praticò Barbato
authors.affiliation: Division of Nephrology, Dialysis and Transplantation, Città della Salute e della Scienza Hospital, Torino, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Fop
authors.affiliation: Department of Medical Sciences, University of Torino, Torino, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Biancone
authors.affiliation: Department of Medical Sciences, University of Torino, Torino, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Camussi
authors.affiliation: Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Bussolati
mesh_terms: D000071916:AC133 Antigen; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000652:urine*; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000473:pathology / Q000503:physiopathology / Q000652:urine; D007678:Kidney Glomerulus / Q000378:metabolism / Q000503:physiopathology; D016030:Kidney Transplantation; D007684:Kidney Tubules / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000652:urine*; D013997:Time Factors
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides
references.citation: J Am Soc Nephrol. 2009 Feb;20(2):278-88
references.pmid: 19158352
references.citation: Clin Lab Med. 1993 Mar;13(1):131-4
references.pmid: 8462256
references.citation: J Am Soc Nephrol. 2006 Sep;17(9):2443-56
references.pmid: 16885410
references.citation: FEBS Lett. 2010 May 3;584(9):1659-64
references.pmid: 20122930
references.citation: Am J Physiol Renal Physiol. 2009 Oct;297(4):F1006-16
references.pmid: 19640902
references.citation: J Cell Sci. 2005 Jul 1;118(Pt 13):2849-58
references.pmid: 15976444
references.citation: J Am Soc Nephrol. 1999 Dec;10(12):2591-8
references.pmid: 10589699
references.citation: Kidney Int. 2006 Nov;70(10):1847-57
references.pmid: 17021608
references.citation: Am J Pathol. 2005 Feb;166(2):545-55
references.pmid: 15681837
references.citation: Stem Cells Transl Med. 2013 Dec;2(12):1011-9
references.pmid: 24167320
references.citation: PLoS One. 2013 Sep 19;8(9):e75054
references.pmid: 24069378
references.citation: Histol Histopathol. 2011 Feb;26(2):223-32
references.pmid: 21154236
references.citation: Kidney Blood Press Res. 2013;37(2-3):116-23
references.pmid: 23594936
references.citation: Kidney Int. 2011 Dec;80(11):1138-45
references.pmid: 21881557
references.citation: Lancet. 2004 Nov 13-19;364(9447):1814-27
references.pmid: 15541456
references.citation: Hum Pathol. 2011 Aug;42(8):1132-41
references.pmid: 21315412
references.citation: Anal Biochem. 1984 Apr;138(1):141-3
references.pmid: 6731838
references.citation: N Engl J Med. 2003 Jul 10;349(2):182-4
references.pmid: 12853592
references.citation: J Pathol. 2013 Apr;229(5):645-59
references.pmid: 23124355
references.citation: PLoS One. 2009;4(4):e5219
references.pmid: 19381331
references.citation: Am J Transplant. 2002 Sep;2(8):701-11
references.pmid: 12243491
references.citation: Biochem Pharmacol. 2011 May 15;81(10):1171-82
references.pmid: 21371441
references.citation: Kidney Int. 2007 Nov;72(9):1095-102
references.pmid: 17700640
references.citation: J Cell Biol. 2013 Feb 18;200(4):373-83
references.pmid: 23420871
references.citation: Transplantation. 2008 Apr 27;85(8):1112-9
references.pmid: 18431230
references.citation: Am J Physiol Renal Physiol. 2012 Jan 1;302(1):F116-28
references.pmid: 21900452
references.citation: Biochim Biophys Acta. 2012 Jul;1820(7):940-8
references.pmid: 22503788
references.citation: J Extracell Vesicles. 2013 Jan 10;2:
references.pmid: 24009896
references.citation: EMBO Mol Med. 2011 Jul;3(7):398-409
references.pmid: 21591261
references.citation: Kidney Int. 2008 Sep;74(5):613-21
references.pmid: 18509321
references.citation: J Immunol Methods. 2014 Jan 31;403(1-2):62-5
references.pmid: 24291342
references.citation: Epilepsy Res. 2012 Mar;99(1-2):101-6
references.pmid: 22130040
references.citation: Stem Cells. 2008 Mar;26(3):698-705
references.pmid: 18096722
references.citation: Kidney Int. 2014 May;85(5):1225-37
references.pmid: 24196483
references.citation: Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13368-73
references.pmid: 15326289