title: Thromboxane A2 stimulates neurite outgrowth in cerebral cortical neurons via mitogen activated protein kinase signaling.
abstract: Thromboxane A2 (TXA2) is a central prostanoid in the cardiovascular system and is a crucial mediator of vascular homeostasis and platelet aggregation. In this study, we report a novel role for TXA2 in neurite outgrowth. TXA2 receptor is expressed in rat cortical neurons, and treatment with the TXA2 agonist U-46619 promotes neurite outgrowth in a concentration-dependent manner. We investigated the molecular mechanism underlying U-46619-induced neurite outgrowth in cortical neurons. Blockade of the phosphorylation of mitogen activated protein kinase (MAPK) prevents U-46619-mediated neurite outgrowth. These data indicates that TXA2 functions as a positive regulator of neurite outgrowth via a mechanism dependent on MAPKs in cortical neurons.
authors.affiliation: Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, 5, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Sumimoto
authors.affiliation: Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, 5, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan; Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, 5, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan. Electronic address: muramatsu@molneu.med.osaka-u.ac.jp.
authors.forename: Rieko
authors.initials: R
authors.lastname: Muramatsu
authors.affiliation: Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, 5, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan. Electronic address: yamashita@molneu.med.osaka-u.ac.jp.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D007150:Immunohistochemistry; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology*; D016501:Neurites / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology; D013928:Thromboxane A2 / Q000378:metabolism*
chemical_list: D013928:Thromboxane A2; D020928:Mitogen-Activated Protein Kinases
keywords: Axon guidance; Development; Neuron; Outgrowth; Regeneration