title: Ten years of next-generation sequencing technology.
abstract: Ten years ago next-generation sequencing (NGS) technologies appeared on the market. During the past decade, tremendous progress has been made in terms of speed, read length, and throughput, along with a sharp reduction in per-base cost. Together, these advances democratized NGS and paved the way for the development of a large number of novel NGS applications in basic science as well as in translational research areas such as clinical diagnostics, agrigenomics, and forensic science. Here we provide an overview of the evolution of NGS and discuss the most significant improvements in sequencing technologies and library preparation protocols. We also explore the current landscape of NGS applications and provide a perspective for future developments.
authors.affiliation: Centre de Génétique Moléculaire - CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France. Electronic address: vandijk@cgm.cnrs-gif.fr.
authors.forename: Erwin L
authors.initials: EL
authors.lastname: van Dijk
authors.affiliation: Centre de Génétique Moléculaire - CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Auger
authors.affiliation: Plateforme Intégrée IMAGIF - CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jaszczyszyn
authors.affiliation: Centre de Génétique Moléculaire - CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Thermes
mesh_terms: D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D057170:Translational Research, Biomedical
keywords: ChIP-seq; DNA-seq; NGS library preparation; Next-generation sequencing (NGS); RNA-seq; genomics