title: MicroRNA-124 inhibits cellular proliferation and invasion by targeting Ets-1 in breast cancer.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that, by targeting certain messenger RNAs (mRNAs) for translational repression or cleavage, can regulate the expression of these genes. In addition, miRNAs may also function as oncogenes and tumor-suppressor genes, as the abnormal expression of miRNAs is associated with various human tumors. However, the effects of the expression of miR-124 in breast cancer remain unclear. The present study was conducted to study the expression of miR-124 in breast cancer, paying particular attention to miR-124's relation to the proliferation, invasion, and apoptosis in breast cancer cell MCF-7 and MDA-MB-231. Real-time quantitative RT-PCR (qRT-PCR) was performed to identify miR-124 that was down-regulated in breast cancer tissues. We also showed E26 transformation specific-1 (Ets-1) and miR-124 expression levels in breast cancer tissues that were associated with lymph node metastases. With transfected synthetic miR-124 agomir into MCF-7 and MDA-MB-231, a significant reduction (P < 0.05) in MCF-7 and MDA-MB-231 cell proliferation and colony forming potential was observed after treatment with miR-124. Apoptosis and migration rates were found to be significantly higher in two breast-derived cell lines transfected with a miR-124 agomir (P < 0.05). Luciferase reporter assay and Western blot were used to verify Ets-1 as a potential major target gene of miR-124, and the result showed that miR-124 can bind to putative binding sites within the Ets-1 mRNA 3' untranslated region (UTR) to reduce its expression. Based on these findings, we propose that miR-124 and Ets-1 may serve as a therapeutic agent in breast cancer.
authors.affiliation: Department of Breast Surgery, The People's Hospital of Henan Province (The People's Hospital of Zhengzhou University), No. 7 Weiwu Road, Zhengzhou, 450003, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Li
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Zang
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Du
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Chen
authors.forename: Meng
authors.initials: M
authors.lastname: Deng
authors.forename: Wencong
authors.initials: W
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
authors.forename: Baoping
authors.initials: B
authors.lastname: Zhai
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics*; D050784:Proto-Oncogene Protein c-ets-1 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D020413:3' Untranslated Regions; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; D050784:Proto-Oncogene Protein c-ets-1; D012333:RNA, Messenger
references.citation: Oncogene. 2002 Dec 5;21(55):8506-9
references.pmid: 12466970
references.citation: PLoS One. 2014 Jun 03;9(6):e96767
references.pmid: 24892549
references.citation: Clin Cancer Res. 2008 May 1;14(9):2690-5
references.pmid: 18451233
references.citation: J Cell Biochem. 2004 Apr 1;91(5):896-903
references.pmid: 15034925
references.citation: Nat Rev Genet. 2011 Nov 18;12(12):861-74
references.pmid: 22094949
references.citation: Oncogene. 2000 Dec 18;19(55):6514-23
references.pmid: 11175367
references.citation: Cancer Sci. 2010 Jun;101(6):1463-70
references.pmid: 20384626
references.citation: N Engl J Med. 2003 Dec 4;349(23):2241-52
references.pmid: 14657432
references.citation: J Ovarian Res. 2013 Nov 26;6(1):84
references.pmid: 24279510
references.citation: Int J Dev Biol. 1996 Dec;40(6):1097-108
references.pmid: 9032015
references.citation: PLoS One. 2011 Mar 28;6(3):e18286
references.pmid: 21464941
references.citation: Nature. 2008 Jan 10;451(7175):147-52
references.pmid: 18185580
references.citation: Genome Biol. 2003;4(9):228
references.pmid: 12952528
references.citation: Mol Cell Biochem. 2014 Jul;392(1-2):153-9
references.pmid: 24658854
references.citation: Br J Cancer. 2003 Mar 24;88(6):910-7
references.pmid: 12644829
references.citation: Hum Mol Genet. 2005 Apr 15;14 Spec No 1:R121-32
references.pmid: 15809264
references.citation: Dig Dis Sci. 2013 Mar;58(3):706-14
references.pmid: 23053883
references.citation: Mod Pathol. 2005 Oct;18(10):1305-20
references.pmid: 15933754
references.citation: Cell Growth Differ. 1997 Jun;8(6):655-65
references.pmid: 9185999
references.citation: Cancer Res. 2007 Sep 15;67(18):8699-707
references.pmid: 17875710
references.citation: Future Oncol. 2012 Jun;8(6):697-702
references.pmid: 22764767
references.citation: Histol Histopathol. 2005 Jan;20(1):119-26
references.pmid: 15578430
references.citation: Cell Oncol (Dordr). 2013 Feb;36(1):1-7
references.pmid: 23338821
references.citation: Eur J Biochem. 1993 Jan 15;211(1-2):7-18
references.pmid: 8425553
references.citation: Clin Exp Metastasis. 1997 Sep;15(5):519-26
references.pmid: 9247254
references.citation: World J Gastrointest Pathophysiol. 2014 May 15;5(2):63-70
references.pmid: 24891977
references.citation: Cancer Res. 2008 Jan 1;68(1):26-33
references.pmid: 18172293
references.citation: Cancer Cell. 2008 May;13(5):394-406
references.pmid: 18455123
references.citation: Br J Cancer. 2004 Oct 4;91(7):1308-15
references.pmid: 15365563
references.citation: J Pathol. 2001 May;194(1):43-50
references.pmid: 11329140
references.citation: Eur J Cancer. 2005 Nov;41(16):2462-78
references.pmid: 16213704
references.citation: Cancer Res. 2005 Jul 15;65(14):6029-33
references.pmid: 16024602
references.citation: Cancer Res. 2004 Sep 15;64(18):6740-9
references.pmid: 15374992
references.citation: Nat Rev Cancer. 2006 Nov;6(11):857-66
references.pmid: 17060945
references.citation: Cell. 1983 Nov;35(1):79-87
references.pmid: 6313230
references.citation: Nucleic Acids Res. 2011 Jan;39(Database issue):D152-7
references.pmid: 21037258
references.citation: Mol Cancer. 2013 Dec 13;12:163
references.pmid: 24330780
references.citation: Oncogene. 2000 Dec 18;19(55):6432-42
references.pmid: 11175359