title: Simultaneous expression of cancer stem cell-like properties and cancer-associated fibroblast-like properties in a primary culture of breast cancer cells.
abstract: The importance of cancer-associated fibroblasts (CAFs) in cancer biology has been recently highlighted owing to their critical roles in cancer growth, progression, metastasis, and therapeutic resistance. We have previously established a primary culture of breast cancer cells, which showed epithelial-mesenchymal transition and cancer stem cell-like properties. In this study, we found that the primary culture also showed CAF-like properties. For example, hypoxia inducible factor 1α (HIF1A) and its downstream genes, nuclear factor-kappa B2 (NF-κB2) and BCL2/adenovirus E1B 19 kd-interacting protein 3 (BNIP3), and many enzymes involved in glycolysis, such as GAPDH, LDH, PGAM1, and PKM2, were highly overexpressed in the primary culture. Moreover, media conditioned with the primary culture cells enhanced the growth of breast cancer cells. Similar to previous CAF studies, this enhancement suggested to be occurred through fibroblast growth factor signaling. This MCKH primary culture cell, which showed simultaneous expression of tumorigenic and CAF properties, offers a unique experimental system for studying the biology of CAFs.
authors.affiliation: Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe 650-0047, Japan. m1461001@center.konan-u.ac.jp.
authors.forename: Mami
authors.initials: M
authors.lastname: Ishikawa
authors.affiliation: Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe 650-0047, Japan. m1461002@center.konan-u.ac.jp.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Inoue
authors.affiliation: Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe 650-0047, Japan. sp091023@center.konan-u.ac.jp.
authors.forename: Takuma
authors.initials: T
authors.lastname: Shirai
authors.affiliation: Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe 650-0047, Japan. kazuhiko.takamatsu@jp.astellas.com.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Takamatsu
authors.affiliation: Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe 650-0047, Japan. m1361007@center.konan-u.ac.jp.
authors.forename: Shiori
authors.initials: S
authors.lastname: Kunihiro
authors.affiliation: Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe 650-0047, Japan. dp161001@center.konan-u.ac.jp.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ishii
authors.affiliation: Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe 650-0047, Japan. nisikata@konan-u.ac.jp.
authors.forename: Takahito
authors.initials: T
authors.lastname: Nishikata
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