title: MALDI-MS lipid profiles of oocytes recovered by ovum pickup from Bos indicus and 1/2 indicus × taurus with high vs low oocyte yields.
abstract: The aim of the present study was to compare the lipid profile in oocytes of indicus and 1/2 indicus × taurus cows with high and low antral follicle count (AFC)/oocyte yields. After an OPU procedure (D0), antral follicles ≥3 mm were counted by ultrasonography (D4, 19, 34, 49, 64), and cows were assigned to groups with either high AFC (≥30 follicles; indicus, NH group; 1/2 indicus × taurus, AH group) or low AFC (≤15 antral follicles; indicus, NL group; 1/2 indicus × taurus, AL group). The lipid profiles of the oocytes were determined by MALDI-MS. For GI, GII and GIII oocytes, the indicus samples tend to cluster separately from the 1/2 indicus × taurus samples. The lipid species [PC (P-38:5) + H](+) and/or [PC (P-36:2) + Na](+) , [PC (38:2) + H](+) , [PC (38:5) + Na](+) and [TAG (60:8) + NH(4) ](+) were more abundant in indicus (NH and NL groups) than 1/2 indicus × taurus. The higher lipid content in the indicus oocytes likely reflects differences in the rate of lipid metabolism and may contribute to oocyte competence and embryo development.
authors.affiliation: Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina, Brazil.
authors.forename: K C
authors.initials: KC
authors.lastname: Silva-Santos
authors.forename: C R
authors.initials: CR
authors.lastname: Ferreira
authors.forename: G M G
authors.initials: GM
authors.lastname: Santos
authors.forename: M N
authors.initials: MN
authors.lastname: Eberlin
authors.forename: L S
authors.initials: LS
authors.lastname: Siloto
authors.forename: C O
authors.initials: CO
authors.lastname: Rosa
authors.forename: T N
authors.initials: TN
authors.lastname: Marcantonio
authors.forename: M M
authors.initials: MM
authors.lastname: Seneda
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics / Q000502:physiology*; D005260:Female; D006824:Hybridization, Genetic; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D009865:Oocytes / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008055:Lipids