title: Atmospheric-pressure plasma jet induces DNA double-strand breaks that require a Rad51-mediated homologous recombination for repair in Saccharomyces cerevisiae.
abstract: Non-thermal plasma generated under atmospheric pressure produces a mixture of chemically reactive molecules and has been developed for a number of biomedical applications. Recently, plasma jet has been proposed as novel cancer therapies based on the observation that free radicals generated by plasma jet induce mitochondria-mediated apoptotic cell death. We show here that air plasma jet induces DNA double-strand breaks (DSBs) in yeast chromosomes leading to genomic instability and loss of viability, which are alleviated by Rad51, the yeast homolog of Escherichiacoli RecA recombinase, through DNA damage repair by a homologous recombination (HR) process. Hypersensitivity of rad51 mutant to air plasma was not restored by antioxidant treatment unlike sod1 mutant that was highly sensitive to reactive oxygen species (ROS) challenge, suggesting that plasma jet induces DSB-mediated cell death independent of ROS generation. These results may provide a new insight into the mechanism of air plasma jet-induced cell death.
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 132-714, Republic of Korea.
authors.forename: Yoonna
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Electrical and Computer Engineering, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: Kangil
authors.initials: K
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 132-714, Republic of Korea.
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Kang
authors.affiliation: Department of Life Sciences, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Electrical and Computer Engineering, Ajou University, Suwon 443-749, Republic of Korea. Electronic address: ssyang@ajou.ac.kr.
authors.forename: Sang Sik
authors.initials: SS
authors.lastname: Yang
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 132-714, Republic of Korea. Electronic address: whchung23@duksung.ac.kr.
authors.forename: Woo-Hyun
authors.initials: WH
authors.lastname: Chung
mesh_terms: D001274:Atmospheric Pressure; D016923:Cell Death / Q000187:drug effects; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D059765:Homologous Recombination / Q000187:drug effects*; D016296:Mutagenesis; D009154:Mutation; D009584:Nitrogen / Q000494:pharmacology; D058626:Plasma Gases / Q000494:pharmacology*; D051135:Rad51 Recombinase / Q000378:metabolism*; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D058626:Plasma Gases; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; C495151:RAD51 protein, S cerevisiae; D051135:Rad51 Recombinase; D009584:Nitrogen
keywords: Double-strand break; Homologous recombination; Plasma jet; Rad51; Reactive oxygen species