title: Molecular characterization and differential expression of olfactory genes in the antennae of the black cutworm moth Agrotis ipsilon.
abstract: Insects use their sensitive and selective olfactory system to detect outside chemical odorants, such as female sex pheromones and host plant volatiles. Several groups of olfactory proteins participate in the odorant detection process, including odorant binding proteins (OBPs), chemosensory proteins (CSPs), odorant receptors (ORs), ionotropic receptors (IRs) and sensory neuron membrane proteins (SNMPs). The identification and functional characterization of these olfactory proteins will enhance our knowledge of the molecular basis of insect chemoreception. In this study, we report the identification and differential expression profiles of these olfactory genes in the black cutworm moth Agrotis ipsilon. In total, 33 OBPs, 12 CSPs, 42 ORs, 24 IRs, 2 SNMPs and 1 gustatory receptor (GR) were annotated from the A. ipsilon antennal transcriptomes, and further RT-PCR and RT-qPCR revealed that 22 OBPs, 3 CSPs, 35 ORs, 14 IRs and the 2 SNMPs are uniquely or primarily expressed in the male and female antennae. Furthermore, one OBP (AipsOBP6) and one CSP (AipsCSP2) were exclusively expressed in the female sex pheromone gland. These antennae-enriched OBPs, CSPs, ORs, IRs and SNMPs were suggested to be responsible for pheromone and general odorant detection and thus could be meaningful target genes for us to study their biological functions in vivo and in vitro.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Gu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Ruo-Nan
authors.initials: RN
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Kong-Ming
authors.initials: KM
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yu-Yuan
authors.initials: YY
authors.lastname: Guo
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xian-Chun
authors.initials: XC
authors.lastname: Li
authors.affiliation: Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, United Kingdom.
authors.forename: Jing-Jiang
authors.initials: JJ
authors.lastname: Zhou
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism*; D016000:Cluster Analysis; D019295:Computational Biology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017344:Genes, Insect; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D058977:Molecular Sequence Annotation; D009036:Moths / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D018035:Receptors, Odorant / Q000235:genetics; D011984:Sensory Receptor Cells / Q000378:metabolism; D059467:Transcriptome
chemical_list: D008565:Membrane Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein
references.citation: Genomics. 1999 Aug 15;60(1):31-9
references.pmid: 10458908
references.citation: BMC Genomics. 2007 Nov 13;8:416
references.pmid: 17997864
references.citation: Nature. 1981 Sep 10-16;293(5828):161-3
references.pmid: 18074618
references.citation: PLoS Genet. 2011 Feb 03;7(2):e1001291
references.pmid: 21304893
references.citation: J Mol Biol. 2004 Jul 16;340(4):783-95
references.pmid: 15223320
references.citation: Neuron. 1999 Feb;22(2):327-38
references.pmid: 10069338
references.citation: Insect Biochem Mol Biol. 1998 Oct;28(10):785-90
references.pmid: 9807224
references.citation: BMC Genomics. 2009 Jul 23;10:332
references.pmid: 19624863
references.citation: Mol Biol Evol. 2009 Sep;26(9):2073-86
references.pmid: 19542205
references.citation: Chem Senses. 2011 May;36(4):335-44
references.pmid: 21220518
references.citation: Insect Biochem Mol Biol. 2007 Mar;37(3):266-77
references.pmid: 17296501
references.citation: Insect Biochem Mol Biol. 2013 Mar;43(3):237-51
references.pmid: 23298680
references.citation: PLoS One. 2013;8(4):e60263
references.pmid: 23565215
references.citation: PLoS One. 2012;7(2):e31620
references.pmid: 22363688
references.citation: Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10996-1001
references.pmid: 18653762
references.citation: Insect Mol Biol. 2009 Jun;18(3):395-404
references.pmid: 19523071
references.citation: BMC Genomics. 2010 Jan 14;11:29
references.pmid: 20074338
references.citation: Cell. 1999 Mar 5;96(5):725-36
references.pmid: 10089887
references.citation: PLoS Genet. 2010 Aug 19;6(8):e1001064
references.pmid: 20808886
references.citation: Int J Biol Sci. 2012;8(7):1036-50
references.pmid: 22904672
references.citation: Insect Mol Biol. 1999 Nov;8(4):501-18
references.pmid: 10620045
references.citation: Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W293-7
references.pmid: 16845012
references.citation: Insect Biochem Mol Biol. 2008 Apr;38(4):387-97
references.pmid: 18342245
references.citation: Science. 2005 Mar 11;307(5715):1638-42
references.pmid: 15692016
references.citation: Vitam Horm. 2010;83:241-72
references.pmid: 20831949
references.citation: Cell. 2009 Jan 9;136(1):149-62
references.pmid: 19135896
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Nat Genet. 2000 May;25(1):25-9
references.pmid: 10802651
references.citation: Chem Senses. 2008 Mar;33(3):291-9
references.pmid: 18209018
references.citation: Cell Mol Life Sci. 2005 May;62(10):1156-66
references.pmid: 15928808
references.citation: Insect Mol Biol. 2003 Apr;12(2):125-34
references.pmid: 12653934
references.citation: BMC Genomics. 2011 May 27;12:271
references.pmid: 21619637
references.citation: Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11845-50
references.pmid: 15289611
references.citation: Insect Mol Biol. 2012 Feb;21(1):41-8
references.pmid: 22074189
references.citation: J Chem Ecol. 2004 May;30(5):889-911
references.pmid: 15274438
references.citation: Genome Res. 2002 Sep;12(9):1357-69
references.pmid: 12213773
references.citation: Chem Senses. 2002 Mar;27(3):231-44
references.pmid: 11923186
references.citation: Nature. 2007 Nov 8;450(7167):289-93
references.pmid: 17943085
references.citation: Bioinformatics. 2001 Dec;17(12):1093-104
references.pmid: 11751217
references.citation: Curr Biol. 2007 Apr 3;17(7):606-12
references.pmid: 17363256
references.citation: Science. 2002 Jan 11;295(5553):328-32
references.pmid: 11711637
references.citation: Neuron. 2005 Jan 20;45(2):193-200
references.pmid: 15664171
references.citation: J Biol Chem. 1994 Jun 10;269(23):16340-7
references.pmid: 8206941
references.citation: Insect Biochem Mol Biol. 2009 Jul;39(7):448-56
references.pmid: 19364529
references.citation: Genome Res. 2006 Nov;16(11):1395-403
references.pmid: 17065611
references.citation: Nature. 2008 Apr 24;452(7190):1007-11
references.pmid: 18408711
references.citation: J Insect Physiol. 2013 Apr;59(4):430-43
references.pmid: 23454276
references.citation: Gene. 1997 Nov 20;202(1-2):39-43
references.pmid: 9427543
references.citation: Insect Mol Biol. 2011 Apr;20(2):189-99
references.pmid: 21091811
references.citation: J Comp Neurol. 2004 Jul 19;475(2):202-10
references.pmid: 15211461
references.citation: Chem Senses. 2001 Feb;26(2):207-13
references.pmid: 11238253
references.citation: Cell Tissue Res. 2001 Mar;303(3):433-46
references.pmid: 11320659
references.citation: Insect Mol Biol. 2007 Oct;16(5):525-37
references.pmid: 17635615
references.citation: Annu Rev Neurosci. 1986;9:121-45
references.pmid: 3518584
references.citation: Insect Mol Biol. 2003 Dec;12(6):549-60
references.pmid: 14986916
references.citation: Bioinformatics. 1998;14(4):349-56
references.pmid: 9632830
references.citation: BMC Genomics. 2009 May 12;10:219
references.pmid: 19435504
references.citation: Eur J Biochem. 1999 Jun;262(3):745-54
references.pmid: 10411636
references.citation: PLoS One. 2012;7(8):e42871
references.pmid: 22900060
references.citation: Genome Res. 2004 Jun;14(6):1147-59
references.pmid: 15140833
references.citation: Science. 2002 Oct 4;298(5591):176-8
references.pmid: 12364795
references.citation: PLoS One. 2013 Sep 20;8(9):e73524
references.pmid: 24073197
references.citation: Bioinformatics. 2005 Sep 15;21(18):3674-6
references.pmid: 16081474
references.citation: Nat Methods. 2008 Jul;5(7):621-8
references.pmid: 18516045
references.citation: Annu Rev Entomol. 2013;58:373-91
references.pmid: 23020622
references.citation: Chem Senses. 2001 Sep;26(7):833-44
references.pmid: 11555479
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: Proc Natl Acad Sci U S A. 2011 May 3;108(18):7449-54
references.pmid: 21498690
references.citation: Insect Mol Biol. 2007 Feb;16(1):107-19
references.pmid: 17257213
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Bioinformatics. 2009 Nov 1;25(21):2839-40
references.pmid: 19737799
references.citation: BMC Genomics. 2013 Sep 22;14:636
references.pmid: 24053512
references.citation: PLoS One. 2012;7(10):e48260
references.pmid: 23110222
references.citation: Genome Res. 1999 Sep;9(9):868-77
references.pmid: 10508846
references.citation: Chem Senses. 2000 Oct;25(5):541-53
references.pmid: 11015326
references.citation: BMC Genomics. 2013 Mar 21;14:198
references.pmid: 23517120
references.citation: J Neurobiol. 1996 May;30(1):3-19
references.pmid: 8727979
references.citation: Insect Mol Biol. 2010 Feb;19 Suppl 1:121-36
references.pmid: 20167023
references.citation: Cell Mol Life Sci. 2006 Jul;63(14):1658-76
references.pmid: 16786224
references.citation: Nature. 2008 Apr 24;452(7190):1002-6
references.pmid: 18408712
references.citation: Cell. 2008 Jun 27;133(7):1255-1265
references.pmid: 18585358
references.citation: PLoS One. 2012;7(11):e50664
references.pmid: 23226348
references.citation: PLoS One. 2013;8(2):e56555
references.pmid: 23424668
references.citation: Int J Dev Biol. 1992 Sep;36(3):391-8
references.pmid: 1445782
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791