title: A dual input DNA-based molecular switch.
abstract: We have designed and characterized a DNA-based molecular switch which processes two physiologically relevant inputs: pH (i.e. alkalinisation) and enzymatic activity, and generates a chemical output (in situ synthesized oligonucleotide). The design, based on allosteric interactions between i-motif and hairpin stem within the DNA molecule, addresses such critical physiological system parameters as molecular simplicity, tunability, orthogonality of the two input sensing domains, and compatibility with intracellular operation/delivery.
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA. inester@lsu.edu.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Nesterova
authors.forename: Siddieg O
authors.initials: SO
authors.lastname: Elsiddieg
authors.forename: Evgueni E
authors.initials: EE
authors.lastname: Nesterov
mesh_terms: D000494:Allosteric Regulation; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D059372:Nucleotide Motifs; D009841:Oligonucleotides / Q000138:chemical synthesis*
chemical_list: D004277:DNA, Single-Stranded; D004798:Enzymes; D009841:Oligonucleotides; D004247:DNA