title: Porcine coronin 1A contributes to nuclear factor-kappa B (NF-κB) inactivation during Haemophilus parasuis infection.
abstract: Haemophilus parasuis (H.parasuis) is the etiological agent of porcine polyserositis and arthritis (Glässer's disease) characterized by fibrinous polyserositis, meningitis and polyarthritis, causing severe economic losses to the swine industry. Currently, the molecular basis of this infection is largely unkonwn. Coronin 1A (Coro1A) plays important roles in host against bacterial infection, yet little is known about porcine Coro1A. In this study, we investigated the molecular characterization of porcine Coro1A, revealing that porcine Coro1A was widely expressed in different tissues. Coro1A could be induced by lipopolysaccharide (LPS), polyinosinic acid-polycytidylic acid [poly (I:C)] and H.parasuis in porcine kidney-15 (PK-15) cells. Functional analyses revealed that porcine Coro1A suppressed the NF-κB activation during H.parasuis infection by inhibiting the degradation of IκBα and nuclear translocation of p65. Overexpression of porcine Coro1A inhibited the transcription of NF-κB-mediated downstream genes [Interleukin-6 (IL-6), Interleukin-8 (IL-8) and COX-2] through down-regulation of NF-κB. The results indicated that porcine Coro1A is an important immunity related gene that helps to inhibit NF-kB activation during H. parasuis infection.
authors.affiliation: Division of Animal Infectious Diseases, State key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P. R. China.
authors.forename: Chong
authors.initials: C
authors.lastname: Liu
authors.affiliation: State key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Division of Animal Infectious Diseases, State key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P. R. China.
authors.forename: Hengling
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Division of Animal Infectious Diseases, State key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P. R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Merial SAS, Lyon, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Charreyre
authors.affiliation: Merial SAS, Lyon, France.
authors.forename: Jean Christophe
authors.initials: JC
authors.lastname: Audonnet
authors.affiliation: Division of Animal Infectious Diseases, State key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P. R. China.
authors.forename: Pin
authors.initials: P
authors.lastname: Chen
authors.affiliation: Division of Animal Infectious Diseases, State key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P. R. China.
authors.forename: Qigai
authors.initials: Q
authors.lastname: He
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006192:Haemophilus Infections / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D044137:Haemophilus parasuis / Q000276:immunology / Q000472:pathogenicity*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins / Q000235:genetics / Q000502:physiology*; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011070:Poly I-C / Q000494:pharmacology; D013552:Swine / Q000235:genetics*; D013553:Swine Diseases / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D008840:Microfilament Proteins; D016328:NF-kappa B; C072061:coronin proteins; D011070:Poly I-C
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