title: Smartphone fluorescence spectroscopy.
abstract: We demonstrate the first use of smartphone spectrophotometry for readout of fluorescence-based biological assays. We evaluated the smartphone fluorimeter in the context of a fluorescent molecular beacon (MB) assay for detection of specific nucleic acid sequences in a liquid test sample and compared performance against a conventional laboratory fluorimeter. The capability of distinguishing a one-point mismatch is also demonstrated by detecting single-base mutation in target nucleic acids. Our approach offers a route toward portable biomolecular assays for viral/bacterial pathogens, disease biomarkers, and toxins.
authors.affiliation: Department of Electrical and Computer Engineering, Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign , 208 North Wright Street, Urbana, Illinois 61801, United States.
authors.forename: Hojeong
authors.initials: H
authors.lastname: Yu
authors.forename: Yafang
authors.initials: Y
authors.lastname: Tan
authors.forename: Brian T
authors.initials: BT
authors.lastname: Cunningham
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D040421:Cell Phone; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics; D015335:Molecular Probes / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D005456:Fluorescent Dyes; D035683:MicroRNAs; D015335:Molecular Probes; D009696:Nucleic Acids