title: New α-glucosidase inhibitors with p-terphenyl skeleton from the mushroom Hydnellum concrescens.
abstract: The purpose of this study is to elucidate the bioactive components responsible for the the α-glucosidase inhibitory activity detected in the EtOAc extract of the mushroom Hydnellum concrescens. Two new p-terphenyl derivatives, concrescenins A (1) and B (2), in along with six known compounds thelephantins L (3), I (4), J (5), K (6), dihydroauran-tiacin dibenzoate (7), and curtisian A (8) were isolated from the fruiting bodies of H. concrescens. Their chemical structures were elucidated by NMR experiments. Compounds 1-4 and 6-8 showed the inhibitory activity against α-glucosidase with the IC50 of 0.99, 3.11, 4.53, 18.77, 2.98, 5.16, and 8.34 μM, respectively. Kinetic analysis of α-glucosidase indicated that compounds 1 and 2 inhibited the activity of α-glucosidase in a noncompetitive fashion with a Ki value of 0.02 and 0.21 μM, respectively. In antioxidant evaluation, compounds 1 and 4 showed weak DPPH scavenging activity (EC50=82.50 and 161.75 μM) and weak reducing ability (EC50=193.57 and 152.94 μM). The current research supports the potential use of mushroom-derived p-terphenyl derivatives for the treatment of diabetes.
authors.affiliation: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, People's Republic of China; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichenxi Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Shi-Mei
authors.initials: SM
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichenxi Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Han
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichenxi Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ma
authors.affiliation: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, People's Republic of China. Electronic address: 13766969566@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichenxi Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Bao
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichenxi Road, Chaoyang District, Beijing 100101, People's Republic of China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Pei
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichenxi Road, Chaoyang District, Beijing 100101, People's Republic of China. Electronic address: liuhw@im.ac.cn.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liu
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D001487:Basidiomycota / Q000737:chemistry*; D002681:China; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D013730:Terphenyl Compounds / Q000737:chemistry* / Q000302:isolation & purification; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D065089:Glycoside Hydrolase Inhibitors; D013730:Terphenyl Compounds; C000597513:concrescenin A; C000597514:concrescenin B; D000520:alpha-Glucosidases
keywords: Antioxidation; Hydnellum concrescens; p-terphenyl derivatives; α-glucosidase inhibitors