title: Proteomics analysis of Mahonia bealei leaves with induction of alkaloids via combinatorial peptide ligand libraries.
abstract: Alkaloids are one of the most attractive sources for obtaining active natural products. However, alkaloids exist in the plants as the secondary metabolites with tracing amount, and there is an enormous demand for a large production. In the present study, we aimed to profile the modification of benzylisoquinoline alkaloids in Mahonia bealei seedlings under the binary stress of ultraviolet-B irradiation and dark incubation. Comparative proteomics analysis was carried out to address the underlying proteome variations that accounted for the alkaloid induction under treatment. Thirteen differential proteins were identified in the leaves under binary stress. Of note, the abundance of S-adenosyl-L-methionine synthetase was highly increased to sustain a high concentration of S-adenosyl-L-methionine for the enhanced biosynthesis of alkaloids. Additionally, we presented the application of CPLL to M. bealei leaf proteins. Three new secondary metabolism proteins and 12 additional differential proteins were identified only after CPLL treatment. Six genes in the benzylisoquinoline alkaloid biosynthesis pathway were selected to verify their variable expression using quantitative real time polymerase chain reaction. The results suggest that the benzylisoquinoline alkaloids in M. bealei leaf were increased to eliminate the adverse effect of UV-B exposure. The suppression of photosynthesis and respiratory rate may save an extra energy for the secondary metabolites, and the enhanced N-metabolism may supply sufficient primary metabolite precursors. To our best knowledge, this is the first work aimed at the secondary metabolism proteomic characterization of M. bealei using the CPLL technique. It also presented an effective and innovative process to improve the contents of alkaloids in medicinal plants for industrial production.
  
  
  CONCLUSIONS
  Besides the effective and innovative process to improve the contents of alkaloids in M. bealei leaves for industrial production, the presented combinatorial hexapeptide ligand library technology was applied for the study of low-abundance protein in medicinal plant. It may be an available tool for the analysis of the secondary proteins.
authors.affiliation: Education Ministry Key Laboratory for Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, Zhejiang 310027, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Education Ministry Key Laboratory for Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China.
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, Zhejiang 310027, China.
authors.forename: Bingxian
authors.initials: B
authors.lastname: Yang
authors.affiliation: College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, Zhejiang 310027, China.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Education Ministry Key Laboratory for Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China.
authors.forename: Ximin
authors.initials: X
authors.lastname: Li
authors.affiliation: Education Ministry Key Laboratory for Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China; College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, Zhejiang 310027, China. Electronic address: tjk@zju.edu.cn.
authors.forename: Jingkui
authors.initials: J
authors.lastname: Tian
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis* / Q000737:chemistry*; D028142:Berberis / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D019151:Peptide Library; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D019151:Peptide Library; D010940:Plant Proteins; D020543:Proteome
keywords: Benzylisoquinoline alkaloids; Combinatorial hexapeptide ligand libraries; Mahonia bealei; Two-dimensional electrophoresis; UV-B irradiation; qRT-PCR