title: Ultrasensitive electrochemical biosensor for detection of DNA from Bacillus subtilis by coupling target-induced strand displacement and nicking endonuclease signal amplification.
abstract: A simple, ultrasensitive, and specific electrochemical biosensor was designed to determine the given DNA sequence of Bacillus subtilis by coupling target-induced strand displacement and nicking endonuclease signal amplification. The target DNA (TD, the DNA sequence from the hypervarient region of 16S rDNA of Bacillus subtilis) could be detected by the differential pulse voltammetry (DPV) in a range from 0.1 fM to 20 fM with the detection limit down to 0.08 fM at the 3s(blank) level. This electrochemical biosensor exhibits high distinction ability to single-base mismatch, double-bases mismatch, and noncomplementary DNA sequence, which may be expected to detect single-base mismatch and single nucleotide polymorphisms (SNPs). Moreover, the applicability of the designed biosensor for detecting the given DNA sequence from Bacillus subtilis was investigated. The result obtained by electrochemical method is approximately consistent with that by a real-time quantitative polymerase chain reaction detecting system (QPCR) with SYBR Green.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, and College of Chemistry, Fuzhou University , Fuzhou, Fujian 350108, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Hu
authors.forename: Xueqin
authors.initials: X
authors.lastname: Xu
authors.forename: Qionghua
authors.initials: Q
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D055664:Electrochemical Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D020641:Polymorphism, Single Nucleotide
chemical_list: D004269:DNA, Bacterial