title: Angiogenin expression during early human placental development; association with blood vessel formation.
abstract: The placenta is a transient organ essential for fetal development. During human placental development, chorionic villi grow in coordination with a large capillary network resulting from both vasculogenesis and angiogenesis. Angiogenin is one of the most potent inducers of neovascularisation in experimental models in vivo. We and others have previously mapped angiogenin expression in the human term placenta. Here, we explored angiogenin involvement in early human placental development. We studied, angiogenin expression by in situ hybridisation and/or by RT-PCR in tissues and primary cultured trophoblastic cells and angiogenin cellular distribution by coimmunolabelling with cell markers: CD31 (PECAM-1), vascular endothelial cadherin (VE-cadherin), vascular endothelial growth factor receptor-2 (VEGF-R2), Tie-2, von Willebrand factor, CD34, erythropoeitin receptor (Epo-R), alpha-smooth muscle actin, CD45, cytokeratin 7, and Ki-67. Extravillous and villous cytotrophoblasts, isolated and differentiated in vitro, expressed and secreted angiogenin. Angiogenin was detected in villous trophoblastic layers, and structured and nascent fetal vessels. In decidua, it was expressed by glandular epithelial cells, vascular cells and macrophages. The observed pattern of angiogenin expression is compatible with a role in blood vessel formation and in cross-talk between trophoblasts and endothelial cells. In view of angiogenin properties, we suggest that angiogenin may participate in placental vasculogenesis and organogenesis.
authors.affiliation: INSERM, U1139, 75006 Paris, France ; Université Paris Descartes, UMR S1139, Sorbonne Paris Cité, 75006 Paris, France ; Université de Lorraine, 54000 Nancy, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Pavlov
authors.affiliation: INSERM, U1139, 75006 Paris, France ; Université Paris Descartes, UMR S1139, Sorbonne Paris Cité, 75006 Paris, France ; CNRS, UMR5547, Université de Toulouse 3, 31062 Toulouse, France.
authors.forename: Jean-Louis
authors.identifier: 0000-0003-0118-5556
authors.initials: JL
authors.lastname: Frendo
authors.affiliation: INSERM, U1139, 75006 Paris, France ; Université Paris Descartes, UMR S1139, Sorbonne Paris Cité, 75006 Paris, France ; Clinical Endocrinology, Hôpital Cochin, AP-HP, Université Paris Descartes, 75006 Paris, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Guibourdenche
authors.affiliation: INSERM, U1139, 75006 Paris, France ; Université Paris Descartes, UMR S1139, Sorbonne Paris Cité, 75006 Paris, France ; PremUP Foundation, 75006 Paris, France.
authors.forename: Séverine A
authors.initials: SA
authors.lastname: Degrelle
authors.affiliation: INSERM, U1139, 75006 Paris, France ; Université Paris Descartes, UMR S1139, Sorbonne Paris Cité, 75006 Paris, France ; PremUP Foundation, 75006 Paris, France.
authors.forename: Danièle
authors.initials: D
authors.lastname: Evain-Brion
authors.affiliation: INSERM, U1139, 75006 Paris, France ; Université Paris Descartes, UMR S1139, Sorbonne Paris Cité, 75006 Paris, France ; INSERM Unit 1139, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, 4 Avenue de l'Observatoire, 75270 Paris, France.
authors.forename: Josette
authors.identifier: 0000-0001-7177-7261
authors.initials: J
authors.lastname: Badet
mesh_terms: D001808:Blood Vessels / Q000254:growth & development / Q000378:metabolism*; D002824:Chorionic Villi / Q000254:growth & development / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D010920:Placenta / Q000378:metabolism; D010929:Placentation; D011247:Pregnancy; D061251:Primary Cell Culture; D012259:Ribonuclease, Pancreatic / Q000096:biosynthesis* / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism
chemical_list: C046366:angiogenin; D012259:Ribonuclease, Pancreatic
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