title: CE-TOF MS-based metabolomic profiling revealed characteristic metabolic pathways in postmortem porcine fast and slow type muscles.
abstract: To determine key compounds and metabolic pathways associated with meat quality, we profiled metabolites in postmortem porcine longissimus lumborum (LL) and vastus intermedius (VI) muscles with different aging times by global metabolomics using capillary electrophoresis-time of flight mass spectrometry. Loading analyses of the principal component analysis showed that hydrophilic amino acids and β-alanine-related compounds contributed to the muscle type positively and negatively, respectively, whereas glycolytic and ATP degradation products contributed to aging time. At 168h postmortem, LL samples were characterized by abundance of combinations of amino acids, dipeptides, and glycolytic products, whereas the VI samples were characterized by abundance of both sulfur-containing compounds and amino acids. The AMP and inosine contents in the VI were approx. 10 times higher than those in the LL at 4h postmortem, suggesting different rates of inosine 5'-monophosphate (IMP) accumulation by adenylate kinase 7 and 5'-nucleotidase, and subsequent different production levels of IMP and hypoxanthine between these two porcine muscles.
authors.affiliation: Animal Products Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan. Electronic address: muros@affrc.go.jp.
authors.forename: Susumu
authors.initials: S
authors.lastname: Muroya
authors.affiliation: Animal Products Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Oe
authors.affiliation: Animal Products Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan.
authors.forename: Ikuyo
authors.initials: I
authors.lastname: Nakajima
authors.affiliation: Animal Products Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Ojima
authors.affiliation: Animal Products Research Division, NARO Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Chikuni
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D005511:Food Handling / Q000379:methods; D063427:Food Quality; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis*; D053858:Metabolic Networks and Pathways; D055432:Metabolomics / Q000379:methods*; D009132:Muscles; D025341:Principal Component Analysis; D013552:Swine; D013997:Time Factors
keywords: CE-TOF MS; Metabolomics; Muscle type; Myosin heavy chain isoform; Pork; Postmortem aging