title: Identification of putative ecdysteroid and juvenile hormone pathway genes in the shrimp Neocaridina denticulata.
abstract: Although the sesquiterpenoid juvenile hormone (JH) and the steroidal ecdysteroids are of vital importance to the development and reproduction of insects, our understanding of the evolution of these crucial hormonal regulators in other arthropods is limited. To better understand arthropod hormone evolution and regulation, here we describe the hormonal pathway genes (e.g. those involved in hormone biosynthesis, degradation, regulation and signal transduction) of a new decapod model, the shrimp Neocaridina denticulata. The majority of known insect sesquiterpenoid and ecdysteroid pathway genes and their regulators are contained in the N. denticulata genome. In the sesquiterpenoid pathway, these include biosynthetic pathway components: juvenile hormone acid methyltransferase (JHAMT); hormone binding protein: juvenile hormone binding protein (JHBP); and degradation pathway components: juvenile hormone esterase (JHE), juvenile hormone esterase binding protein (JHEBP) and juvenile hormone epoxide hydrolase (JHEH), with the JHBP, JHEBP and JHEH genes being discovered in a crustacean for the first time here. Ecdysteroid biosynthetic pathway genes identified include spook, phantom, disembodied, shadow and CYP18. Potential hormonal regulators and signal transducers such as allatostatins (ASTs), Methoprene-tolerant (Met), Retinoid X receptor (RXR), Ecdysone receptor (EcR), calponin-like protein Chd64, FK509-binding protein (FKBP39), Broad-complex (Br-c), and crustacean hyperglycemic hormone/molt-inhibiting hormone/gonad-inhibiting hormone (CHH/MIH/GIH) genes are all present in the shrimp N. denticulata. To our knowledge, this is the first report of these hormonal pathways and their regulatory genes together in a single decapod, providing a vital resource for further research into development, reproduction, endocrinology and evolution of crustaceans, and arthropods in general.
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology and Centre for Soybean Research, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Yung Wa
authors.initials: YW
authors.lastname: Sin
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology and Centre for Soybean Research, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Kenny
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology and Centre for Soybean Research, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Qu
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology and Centre for Soybean Research, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Ka Wo
authors.initials: KW
authors.lastname: Chan
authors.affiliation: The Hong Kong Institute of Vocational Education, Chaiwan, Hong Kong.
authors.forename: Katie W S
authors.initials: KW
authors.lastname: Chan
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology and Centre for Soybean Research, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Sam P S
authors.initials: SP
authors.lastname: Cheong
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology and Centre for Soybean Research, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Ricky W T
authors.initials: RW
authors.lastname: Leung
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology and Centre for Soybean Research, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Ting Fung
authors.initials: TF
authors.lastname: Chan
authors.affiliation: Department of Biology, Queen's University, Kingston, ON, Canada.
authors.forename: William G
authors.initials: WG
authors.lastname: Bendena
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology and Centre for Soybean Research, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Ka Hou
authors.initials: KH
authors.lastname: Chu
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, M5S 3G5, Canada.
authors.forename: Stephen S
authors.initials: SS
authors.lastname: Tobe
authors.affiliation: School of Life Sciences, State Key Laboratory of Agrobiotechnology and Centre for Soybean Research, Chinese University of Hong Kong, Shatin, Hong Kong. Electronic address: jeromehui@cuhk.edu.hk.
authors.forename: Jerome H L
authors.initials: JH
authors.lastname: Hui
mesh_terms: D000818:Animals; D033364:Decapoda / Q000235:genetics* / Q000378:metabolism; D026461:Ecdysteroids / Q000235:genetics* / Q000378:metabolism; D007605:Juvenile Hormones / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D026461:Ecdysteroids; D007605:Juvenile Hormones; D012333:RNA, Messenger
keywords: Arthropod; Crustacean; Ecdysteroid; Insect; Juvenile hormone; Methyl farnesoate