title: Differences of various region-of-interest methods for measuring dopamine transporter availability using 99mTc-TRODAT-1 SPECT.
abstract: This study was to investigate whether various region-of-interest (ROI) methods for measuring dopamine transporter (DAT) availabilities by single photon emission computed tomography (SPECT) are statistically different, whether results of medical research are thereby influenced, and causes of these differences. Eighty-four healthy adults with (99m)Tc-TRODAT-1 SPECT and magnetic resonance imaging (MRI) scans were included. Six major analysis approaches were compared: (1) ROI drawn on the coregistered MRI; (2) ROIs drawn on the SPECT images; (3) standard ROI templates; (4) threshold-ROIs; (5) atlas-based mappings with coregistered MRI; and (6) atlas-based mappings with SPECT images. Using the atlas-based approaches we assessed the influence of striatum ROIs by slice-wise and voxel-wise comparisons. In (5) and (6), three partial-volume correction (PVC) methods were also explored. The results showed that DAT availabilities obtained from different methods were closely related but quite different and leaded to significant differences in determining the declines of DAT availability per decade (range: 5.95-11.99%). Use of 3D whole-striatum or more transverse slices could avoid biases in measuring the striatal DAT declines per decade. Atlas-based methods with PVC may be the preferable methods for medical research.
authors.affiliation: Department of Computer Science and Information Engineering, National University of Kaohsiung, 700 Kaohsiung University Road, Kaohsiung 811, Taiwan.
authors.forename: Tang-Kai
authors.initials: TK
authors.lastname: Yin
authors.affiliation: Department of Nuclear Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 704, Taiwan.
authors.forename: Bi-Fang
authors.initials: BF
authors.lastname: Lee
authors.affiliation: Department of Psychiatry, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 704, Taiwan.
authors.forename: Yen Kuang
authors.initials: YK
authors.lastname: Yang
authors.affiliation: Department of Nuclear Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 704, Taiwan.
authors.forename: Nan-Tsing
authors.initials: NT
authors.lastname: Chiu
mesh_terms: D000328:Adult; D050483:Dopamine Plasma Membrane Transport Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D013667:Technetium; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*; D055815:Young Adult
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D013667:Technetium
references.citation: Neuroimage. 2008 Apr 15;40(3):1195-201
references.pmid: 18282769
references.citation: Neuroimage. 2011 Jun 1;56(3):1526-30
references.pmid: 21371559
references.citation: J Nucl Med. 2010 Sep;51(9):1331-4
references.pmid: 20720060
references.citation: Eur J Nucl Med. 2000 Nov;27(11):1714-8
references.pmid: 11105829
references.citation: J Affect Disord. 2005 Dec;89(1-3):115-23
references.pmid: 16213028
references.citation: Neuroimage. 2008 Mar 1;40(1):275-9
references.pmid: 18096411
references.citation: Neuroimage. 2003 Jul;19(3):1233-9
references.pmid: 12880848
references.citation: Neuroimage. 2013 Jan 1;64:61-7
references.pmid: 22982354
references.citation: Exp Neurol. 2008 Nov;214(1):112-6
references.pmid: 18723016
references.citation: Eur J Nucl Med Mol Imaging. 2011 Jun;38(6):1104-19
references.pmid: 21336694
references.citation: Neuroimage. 2008 Feb 15;39(4):1570-84
references.pmid: 18077187
references.citation: Mol Imaging Biol. 2011 Dec;13(6):1267-71
references.pmid: 20953736
references.citation: J Nucl Med. 2007 Sep;48(9):1459-67
references.pmid: 17704252
references.citation: Nucl Med Commun. 2009 Sep;30(9):713-7
references.pmid: 19550362
references.citation: J Neurol Sci. 2010 Apr 15;291(1-2):64-8
references.pmid: 20096859
references.citation: J Nucl Med Technol. 2010 Jun;38(2):61-8
references.pmid: 20484180
references.citation: J Nucl Med. 2012 Mar;53(3):399-406
references.pmid: 22323779
references.citation: Am J Psychiatry. 2007 Apr;164(4):622-9
references.pmid: 17403976
references.citation: Neuroimage. 2004 Jan;21(1):450-5
references.pmid: 14741682
references.citation: J Cereb Blood Flow Metab. 2003 Mar;23(3):285-300
references.pmid: 12621304
references.citation: Nucl Med Commun. 2009 Jul;30(7):513-8
references.pmid: 19451839
references.citation: Neuroimage. 2007 Feb 15;34(4):1782-9
references.pmid: 17188897
references.citation: Neurobiol Dis. 2010 Feb;37(2):356-61
references.pmid: 19853661
references.citation: Clin Neurol Neurosurg. 2008 Jan;110(1):19-24
references.pmid: 17900799
references.citation: Psychiatry Res. 2009 Feb 28;171(2):120-8
references.pmid: 19176281
references.citation: Neurosci Biobehav Rev. 2002 Nov;26(7):785-93
references.pmid: 12470690
references.citation: Neuroimage. 2011 Mar 1;55(1):101-12
references.pmid: 21126591
references.citation: Neuroimage. 2003 Jul;19(3):601-12
references.pmid: 12880791