title: [Studies on optimizing extraction technics of flowers of Polygonum orientale by response surface methodology].
abstract: OBJECTIVE
  To analyze and optimize extraction technics of Polygonum orientale flowers by response surface methodology.
  
  
  METHODS
  With the index for the content of taxifolin in flowers of Polygonum orientale, the effect of three factors such as concentration of alcohol, extraction time and solvent-solid ratio was designed by Box-Behnken central composite. Extraction technic parameters of Polygonum orientale flowers was optimized by response surface methodology.
  
  
  RESULTS
  The optimizing extraction conditions of Polygonum orientale flowers were as follows: ethanol concentration was 65%, extracting time was 129 min and solvent-solid ratio was 18. Under the conditions, the average yield of taxifolin in 3 validation experiments was 2.79 mg/g.
  
  
  CONCLUSIONS
  Optimizing extraction technics by response surface methodology is reasonable, simple, and has good predictability.
authors.forename: Yu-Yao
authors.initials: YY
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Tong
authors.forename: Tian-Min
authors.initials: TM
authors.lastname: Wang
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Zhai
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D035264:Flowers / Q000737:chemistry*; D016014:Linear Models; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D000431:Ethanol; D011794:Quercetin; C003377:taxifolin