title: Oleuropein attenuates visceral adiposity in high-fat diet-induced obese mice through the modulation of WNT10b- and galanin-mediated signalings.
abstract: METHODS
  The aim of the present study was to investigate the antiobesity effect of oleuropein on high-fat diet (HFD) induced body weight gain and visceral adiposity in mice, and to explore the underlying mechanisms involved.
  
  
  RESULTS
  C57BL/6N mice were fed with a normal diet, HFD (40% fat of total energy), and HFD-supplemented with 0.03% oleuropein for 10 wk. Oleuropein significantly reduced HFD-induced body weight gain and visceral adiposity. Oleuropein also significantly reversed the HFD-induced elevations of adipogenic related gene expression involved in WNT10b- and galanin-mediated signalings in adipose tissue of mice. Consistent with in vivo findings, oleuropein dose-dependently suppressed lipid accumulation in 3T3-L1 cells during preadipocyte differentiation. Additionally, exposure of the 3T3-L1 preadipocytes to oleuropein resulted in a marked attenuation of the secreted frizzled-related protein 2 (WNT inhibitor) or galnon (galanin receptor agonist) induced cellular lipid accumulation.
  
  
  CONCLUSIONS
  This study demonstrated the oleuropein-reduced body weight gain and visceral adiposity in HFD-fed mice. The protective effect of oleuropein against HFD-induced adiposity in mice appeared to be mediated through the upregulation of genes involved in WNT10b-mediated signaling and downregulation of genes involved in galanin-mediated signaling cascades.
authors.affiliation: Department of Food and Nutrition, Yonsei University, Seoul, South Korea.
authors.forename: Narae
authors.initials: N
authors.lastname: Kuem
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Song
authors.forename: Rina
authors.initials: R
authors.lastname: Yu
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Yun
authors.forename: Taesun
authors.initials: T
authors.lastname: Park
mesh_terms: D041721:3T3-L1 Cells; D050154:Adiposity / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D019004:Galanin / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006949:Hyperlipidemias / Q000517:prevention & control; D057888:Iridoid Glucosides; D039823:Iridoids / Q000494:pharmacology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D015398:Signal Transduction; D015430:Weight Gain / Q000187:drug effects; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D019004:Galanin; D057888:Iridoid Glucosides; D039823:Iridoids; D008565:Membrane Proteins; D051153:Wnt Proteins; C491628:Sfrp2 protein, mouse; D000094742:Secreted Frizzled-Related Proteins; C102068:Wnt10b protein, mouse; C002769:oleuropein
keywords: Adipogenesis; Galanin-mediated signaling; Mice; Oleuropein; WNT10b-mediated signaling