title: Overexpression of receptor for advanced glycation end products and high-mobility group box 1 in human dental pulp inflammation.
abstract: High mobility group box 1 (HMGB1), a nonhistone DNA-binding protein, is released into the extracellular space and promotes inflammation. HMGB1 binds to related cell signaling transduction receptors, including receptor for advanced glycation end products (RAGE), which actively participate in vascular and inflammatory diseases. The aim of this study was to examine whether RAGE and HMGB1 are involved in the pathogenesis of pulpitis and investigate the effect of Prevotella intermedia (P. intermedia) lipopolysaccharide (LPS) on RAGE and HMGB1 expression in odontoblast-like cells (OLC-1). RAGE and HMGB1 expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. Upregulated expression of RAGE was observed in odontoblasts, stromal pulp fibroblasts-like cells, and endothelial-like cell lining human pulpitis tissue. Strong cytoplasmic HMGB1 immunoreactivity was noted in odontoblasts, whereas nuclear HMGB1 immunoreactivity was seen in stromal pulp fibroblasts-like cells in human pulpitis tissue. LPS stimulated OLC-1 cells produced HMGB1 in a dose-dependent manner through RAGE. HMGB1 translocation towards the cytoplasm and secretion from OLC-1 in response to LPS was inhibited by TPCA-1, an inhibitor of NF-κB activation. These findings suggest that RAGE and HMGB1 play an important role in the pulpal immune response to oral bacterial infection.
authors.affiliation: Department of Pharmacology, Faculty of Dentistry, Mahidol University, 6 Yothe Road, Rajthevee, Bangkok 10400, Thailand.
authors.forename: Salunya
authors.initials: S
authors.lastname: Tancharoen
authors.affiliation: Department of Advanced General Dentistry, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Tassanee
authors.initials: T
authors.lastname: Tengrungsun
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Theeralaksna
authors.initials: T
authors.lastname: Suddhasthira
authors.affiliation: Departments of Physiology and Neurosurgery, Kurume University School of Medicine, Fukuoka 830-0011, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kikuchi
authors.affiliation: Department of Public Health and Environment, Nakhon Pathom Municipality, Nakhon Pathom 73000, Thailand.
authors.forename: Nuttavun
authors.initials: N
authors.lastname: Vechvongvan
authors.affiliation: Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan.
authors.forename: Masayuki
authors.identifier: 0000-0001-6682-0720
authors.initials: M
authors.lastname: Tokuda
authors.affiliation: Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
authors.forename: Ikuro
authors.initials: I
authors.lastname: Maruyama
mesh_terms: D002478:Cells, Cultured; D003782:Dental Pulp / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000235:genetics / Q000378:metabolism*; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*
chemical_list: D024243:HMGB1 Protein; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic
references.citation: Infect Immun. 2001 Aug;69(8):4951-7
references.pmid: 11447173
references.citation: Arthritis Rheum. 2003 Apr;48(4):971-81
references.pmid: 12687539
references.citation: J Clin Invest. 2001 Oct;108(7):949-55
references.pmid: 11581294
references.citation: Blood. 2003 Apr 1;101(7):2652-60
references.pmid: 12456506
references.citation: Semin Thromb Hemost. 2000;26(5):485-93
references.pmid: 11129404
references.citation: Mediators Inflamm. 2013;2013:596716
references.pmid: 24302816
references.citation: Cell Commun Signal. 2009 May 08;7:12
references.pmid: 19426472
references.citation: Clin Exp Rheumatol. 2007 Sep-Oct;25(5):740-2
references.pmid: 18078623
references.citation: J Exp Med. 2000 Aug 21;192(4):565-70
references.pmid: 10952726
references.citation: Annu Rev Med. 1987;38:417-32
references.pmid: 3555304
references.citation: J Mol Med (Berl). 2005 Nov;83(11):876-86
references.pmid: 16133426
references.citation: Int Rev Immunol. 1990;6(4):247-61
references.pmid: 2102906
references.citation: Cell. 1999 Jun 25;97(7):889-901
references.pmid: 10399917
references.citation: J Periodontal Res. 2008 Feb;43(1):76-83
references.pmid: 18067550
references.citation: Ann N Y Acad Sci. 2011 Dec;1243:88-102
references.pmid: 22211895
references.citation: J Biol Chem. 2007 Dec 14;282(50):36330-40
references.pmid: 17942394
references.citation: J Leukoc Biol. 2009 Sep;86(3):645-53
references.pmid: 19581374
references.citation: Br J Pharmacol. 2005 May;145(2):178-92
references.pmid: 15753951
references.citation: Front Physiol. 2013 Nov 11;4:326
references.pmid: 24273514
references.citation: J Physiol. 2006 May 15;573(Pt 1):225-36
references.pmid: 16527857
references.citation: J Immunol. 2006 Mar 1;176(5):2880-7
references.pmid: 16493045
references.citation: Biochem Biophys Res Commun. 2006 Apr 14;342(3):718-24
references.pmid: 16494847
references.citation: Lab Invest. 2010 Jun;90(6):853-66
references.pmid: 20231821
references.citation: Arch Oral Biol. 2002 Dec;47(12):859-66
references.pmid: 12450517
references.citation: Front Biosci. 2001 Oct 01;6:D1151-60
references.pmid: 11578972
references.citation: Biochim Biophys Acta. 2005 Sep 25;1741(3):271-81
references.pmid: 15869869
references.citation: J Alzheimers Dis. 2009;16(4):833-43
references.pmid: 19387116
references.citation: Adv Dent Res. 1988 Nov;2(2):284-91
references.pmid: 3271023
references.citation: J Endod. 2006 Oct;32(10):951-5
references.pmid: 16982271
references.citation: Br J Nutr. 2014 Apr 14;111(7):1221-30
references.pmid: 24252501
references.citation: Exp Ther Med. 2011 Sep;2(5):767-770
references.pmid: 22977572
references.citation: J Biol Chem. 1992 Jul 25;267(21):14998-5004
references.pmid: 1378843
references.citation: Arch Oral Biol. 2003 Apr;48(4):273-83
references.pmid: 12663072
references.citation: J Immunol. 2010 Apr 15;184(8):4497-509
references.pmid: 20231695
references.citation: World J Gastroenterol. 2006 Dec 21;12(47):7666-70
references.pmid: 17171797
references.citation: Int Endod J. 2012 May;45(5):439-48
references.pmid: 22188368
references.citation: Rheumatology (Oxford). 2013 Oct;52(10):1739-47
references.pmid: 23584368
references.citation: EMBO Rep. 2002 Oct;3(10):995-1001
references.pmid: 12231511
references.citation: J Infect Dis. 2001 Dec 15;184(12):1566-71
references.pmid: 11740732
references.citation: Genome Res. 1996 Oct;6(10):986-94
references.pmid: 8908518
references.citation: Am J Respir Crit Care Med. 2008 Aug 15;178(4):356-62
references.pmid: 18535257
references.citation: Infect Immun. 1999 Apr;67(4):1736-42
references.pmid: 10085012
references.citation: Infect Immun. 1986 Jul;53(1):149-53
references.pmid: 3721577
references.citation: J Biol Chem. 1995 Oct 27;270(43):25752-61
references.pmid: 7592757
references.citation: J Endod. 2004 Nov;30(11):770-3
references.pmid: 15505507
references.citation: Pain. 2001 Dec;94(3):231-244
references.pmid: 11731060
references.citation: Am J Physiol Lung Cell Mol Physiol. 2011 Apr;300(4):L516-25
references.pmid: 21257730
references.citation: Toxicology. 2013 Jun 7;308:138-45
references.pmid: 23578390
references.citation: Int Immunol. 2005 Jan;17(1):1-14
references.pmid: 15585605
references.citation: J Endod. 1996 Jan;22(1):9-12
references.pmid: 8618087
references.citation: Int J Mol Sci. 2013 Sep 13;14(9):18899-924
references.pmid: 24065095
references.citation: J Endod. 2004 Feb;30(2):88-91
references.pmid: 14977303
references.citation: Oral Microbiol Immunol. 2007 Feb;22(1):5-13
references.pmid: 17241164
references.citation: J Endod. 2001 Apr;27(4):273-7
references.pmid: 11485266
references.citation: Oral Microbiol Immunol. 2009 Aug;24(4):292-8
references.pmid: 19572890
references.citation: Ann Neurol. 2012 Sep;72(3):373-84
references.pmid: 22915134
references.citation: Nature. 2002 Jul 11;418(6894):191-5
references.pmid: 12110890
references.citation: J Immunol. 2011 Jul 1;187(1):118-25
references.pmid: 21642542
references.citation: J Pharmacol Exp Ther. 2005 Jan;312(1):373-81
references.pmid: 15316093
references.citation: Crit Rev Oral Biol Med. 1998;9(4):498-521
references.pmid: 9825224
references.citation: Surgery. 1999 Aug;126(2):389-92
references.pmid: 10455911
references.citation: FEBS Lett. 2003 May 22;543(1-3):98-102
references.pmid: 12753913
references.citation: Infect Immun. 1992 Nov;60(11):4932-7
references.pmid: 1328062