title: [HPTLC fingerprint analysis of andrographolides from Andrographis paniculata].
abstract: OBJECTIVE
  To establish the high-performance thin layer chromatography (HPTLC) fingerprint of andrographolides from Andrographis paniculata, and to valuate the fingerprint similarity of samples from different habitats, markets, used parts and so on.
  
  
  METHODS
  Chromatographic conditions were as follows: stationary phase: precoated HPTLC GF254 silica-gel plate (20 cm x 10 cm); developing solvent system: chloroform-toluene-methanol (80:10:15); Relative humidity: 42%; Color development reagent: 5% H2SO4 ethanolic solution, heating at 105 degrees C and observing the fluorescent chromatogram in a UV cabinet at 366 nm. The common patterns of HPTLC fingerprint were obtained through CHROMAP 1.5 solution software.
  
  
  RESULTS
  The HPTLC fingerprint of andrographolides was consisted of 9 characteristic peaks (fluorescent bands) including andrographolide, neoandrographolide and dehydroandrographolide which were chemical reference substances. The investigation and analysis of 51 batches of Andrographis paniculata showed that there were remarkable differences among different samples, so was the content of andrographolide and total lactones.
  
  
  CONCLUSIONS
  This method is simple and rapid, which can serve as an effective identification and quality assessment method for Andrographis paniculata.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Shao
authors.forename: Jian-Gang
authors.initials: JG
authors.lastname: Wang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Lai
authors.forename: Xiang-Wei
authors.initials: XW
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Ding
mesh_terms: D035685:Andrographis / Q000737:chemistry* / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D003890:Desiccation / Q000379:methods; D004224:Diterpenes / Q000737:chemistry*; D004355:Drug Stability; D005960:Glucosides / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D013764:Tetrahydronaphthalenes / Q000737:chemistry
chemical_list: D004224:Diterpenes; D005960:Glucosides; D013764:Tetrahydronaphthalenes; C030419:andrographolide; C478098:dehydroandrographolide; C082497:neoandrographolide