title: Improvement of 2,3-butanediol yield in Klebsiella pneumoniae by deletion of the pyruvate formate-lyase gene.
abstract: Klebsiella pneumoniae is considered a good host strain for the production of 2,3-butanediol, which is a promising platform chemical with various industrial applications. In this study, three genes, including those encoding glucosyltransferase (wabG), lactate dehydrogenase (ldhA), and pyruvate formate-lyase (pflB), were disrupted in K. pneumoniae to reduce both its pathogenic characteristics and the production of several by-products. In flask cultivation with minimal medium, the yield of 2,3-butanediol from rationally engineered K. pneumoniae (ΔwabG ΔldhA ΔpflB) reached 0.461 g/g glucose, which was 92.2% of the theoretical maximum, with a significant reduction in by-product formation. However, the growth rate of the pflB mutant was slightly reduced compared to that of its parental strain. Comparison with similar mutants of Escherichia coli suggested that the growth defect of pflB-deficient K. pneumoniae was caused by redox imbalance rather than reduced level of intracellular acetyl coenzyme A (acetyl-CoA). From an analysis of the transcriptome, it was confirmed that the removal of pflB from K. pneumoniae significantly repressed the expression of genes involved in the formate hydrogen lyase (FHL) system.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Moo-Young
authors.initials: MY
authors.lastname: Jung
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Suman
authors.initials: S
authors.lastname: Mazumdar
authors.affiliation: Macrogen Inc., Seoul, Republic of Korea.
authors.forename: Sang Heum
authors.initials: SH
authors.lastname: Shin
authors.affiliation: Macrogen Inc., Seoul, Republic of Korea.
authors.forename: Kap-Seok
authors.initials: KS
authors.lastname: Yang
authors.affiliation: Department of Chemical and Biological Engineering, Sogang University, Seoul, Republic of Korea.
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea mkoh@korea.ac.kr.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Oh
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D053898:Biosynthetic Pathways; D002072:Butylene Glycols / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D055786:Gene Knockout Techniques; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering; D017384:Sequence Deletion; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D002072:Butylene Glycols; C026978:2,3-butylene glycol; D007770:L-Lactate Dehydrogenase; D000123:Acetyltransferases; C022560:formate C-acetyltransferase; D005964:Glucosyltransferases; D005947:Glucose
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