title: Current perspectives on RNA secondary structure probing.
abstract: The range of roles played by structured RNAs in biological systems is vast. At the same time as we are learning more about the importance of RNA structure, recent advances in reagents, methods and technology mean that RNA secondary structural probing has become faster and more accurate. As a result, the capabilities of laboratories that already perform this type of structural analysis have increased greatly, and it has also become more widely accessible. The present review summarizes established and recently developed techniques. The information we can derive from secondary structural analysis is assessed, together with the areas in which we are likely to see exciting developments in the near future.
authors.affiliation: *University of Cambridge Department of Medicine, Box 157 Level 5 Addenbrooke's Hospital, Hills Road, Cambridge, Cambridgeshire CB2 0QQ, U.K.
authors.forename: Julia
authors.initials: J
authors.lastname: Kenyon
authors.affiliation: *University of Cambridge Department of Medicine, Box 157 Level 5 Addenbrooke's Hospital, Hills Road, Cambridge, Cambridgeshire CB2 0QQ, U.K.
authors.forename: Liam
authors.initials: L
authors.lastname: Prestwood
authors.affiliation: *University of Cambridge Department of Medicine, Box 157 Level 5 Addenbrooke's Hospital, Hills Road, Cambridge, Cambridgeshire CB2 0QQ, U.K.
authors.forename: Andrew
authors.initials: A
authors.lastname: Lever
mesh_terms: D000465:Algorithms; D000818:Animals; D019295:Computational Biology; D006801:Humans; D009690:Nucleic Acid Conformation; D017433:Protein Structure, Secondary; D012313:RNA / Q000737:chemistry*
chemical_list: D012313:RNA
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