title: Commercial formulation of Metarhizium anisopliae for the control of Rhipicephalus microplus in a pen study.
abstract: The present study evaluated, for the first time, the effect of the commercial formulation Metarril(®) SP Organic of Metarhizium anisopliae plus 10% mineral oil to control Rhipicephalus microplus in a pen study. Three groups were formed with six animals each: the first group was exposed to Metarril(®) plus 10% mineral oil and 1% Tween 80; the second group was exposed to sterile distilled water, mineral oil and Tween 80 (oil control group); and the third group received no treatment (control group). The fungal formulation contained 1 × 10(8)conidiaml(-1). Each animal was sprayed with 3L of formulation. Fallen ticks were counted daily and a sample of 20 engorged females per day was incubated for assessment of biological parameters. Throughout the study period, Metarril(®) oil-based formulation showed an efficacy ranging from 19.20% to 67.39% in comparison with the control group; and from 8.18% to 61.38% in comparison with the oil control group. The average efficacy of Metarril(®) oil-based formulation was 47.74% and 40.89% in comparison with control and oil control groups, respectively. Changes in the biological parameters of engorged R. microplus females were observed in the first three days after treatment, with a significant reduction in hatching percentage and egg production index. We concluded that Metarril(®) SP Organic plus 10% mineral oil was efficient against R. microplus in pen studies. However, further in vivo studies are required to increase the efficacy and to establish a protocol for the use of this product in the field against the cattle tick.
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro CEP 23890-000, Brazil.
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Camargo
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro CEP 23890-000, Brazil.
authors.forename: Allan F
authors.initials: AF
authors.lastname: Marciano
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro CEP 23890-000, Brazil.
authors.forename: Fillipe A
authors.initials: FA
authors.lastname: Sá
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro CEP 23890-000, Brazil.
authors.forename: Wendell M S
authors.initials: WM
authors.lastname: Perinotto
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro CEP 23890-000, Brazil.
authors.forename: Simone
authors.initials: S
authors.lastname: Quinelato
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro CEP 23890-000, Brazil.
authors.forename: Patrícia S
authors.initials: PS
authors.lastname: Gôlo
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro CEP 23890-000, Brazil.
authors.forename: Isabele C
authors.initials: IC
authors.lastname: Angelo
authors.affiliation: Embrapa Gado de Leite, Rua Eugênio do Nascimento, 610, Bairro Dom Bosco, Juiz de Fora, Minas Gerais CEP 36038-330, Brazil.
authors.forename: Márcia C A
authors.initials: MC
authors.lastname: Prata
authors.affiliation: Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro CEP 23890-000, Brazil. Electronic address: vaniabit@ufrrj.br.
authors.forename: Vânia R E P
authors.initials: VR
authors.lastname: Bittencourt
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology* / Q000517:prevention & control; D052981:Metarhizium; D010572:Pest Control, Biological / Q000379:methods*; D048494:Rhipicephalus / Q000187:drug effects*; D013983:Tick Control / Q000379:methods*; D013984:Tick Infestations / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*
keywords: Acaripathogenic fungi; Biological control; Cattle tick; Oil formulation; Pen studies