title: Suppressed Krüppel‑like factor 17 expression induces tumor proliferation, metastasis and a poor prognosis in papillary thyroid carcinoma.
abstract: Although the role of krüppel‑like factor 17 (KLF17) in the regulation of proliferation and epithelial‑mesenchymal transition has been examined in breast and liver cancer, their effect on papillary thyroid carcinoma (PTC) remains to be elucidated. The present study aimed to investigate the expression pattern of KLF17 in PTC and the correlation between KLF17 expression and the malignant potential of PTC. KLF17 expression in PTC and adjacent liver tissues was studied by polymerase chain reaction and western blot analysis, and the association between KLF17 expression and the clinicopathological features of PTC was studied in 50 patients. By using RNA interference against KLF17, the correlation between KLF17 expression and malignant potential was examined by downregulating KLF17 expression in TPC‑1 cells, and the effects of KLF17 downregulation on cell proliferation and motility were analyzed. Furthermore, the association between KLF17 expression and the surgical outcomes of PTC patients were analyzed. Downregulated expression of KLF17 was associated with a shorter overall survival time in clinical patients (P<0.05). Low KLF17 expression was significantly associated with tumor stage, tumor size, nodal stage and metastasis stage in PTC (P<0.05). The reduced expression of KLF17 promoted the motility and proliferation ability of TPC‑1 cells by altering the expression of tight junction protein 1 and Snai1, and activating the AKT pathway by upregulating inhibitor of DNA binding 1. In conclusion, the present study demonstrated that KLF17 is important in tumor proliferation and may be a useful prognostic indicator in directing therapy. Therefore, further investigation regarding the role of KLF17 in PTC is required.
authors.affiliation: Department of Endocrinology, Mianyang Central Hospital, Mianyang, Sichuan 621000, P.R. China.
authors.forename: Wen-Chun
authors.initials: WC
authors.lastname: Ye
authors.affiliation: Department of Endocrinology, Mianyang Central Hospital, Mianyang, Sichuan 621000, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Endocrinology, Mianyang Central Hospital, Mianyang, Sichuan 621000, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Endocrinology, Mianyang Central Hospital, Mianyang, Sichuan 621000, P.R. China.
authors.forename: Xiang-Ming
authors.initials: XM
authors.lastname: Fang
authors.affiliation: Department of Pathology, Mianyang Central Hospital, Mianyang, Sichuan 621000, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xie
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D000071250:Snail Family Transcription Factors; D015996:Survival Rate; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; C510638:KLF17 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; C569042:TJP1 protein, human; D014157:Transcription Factors; D062826:Zonula Occludens-1 Protein; D051057:Proto-Oncogene Proteins c-akt