title: Lipid-AuNPs@PDA nanohybrid for MRI/CT imaging and photothermal therapy of hepatocellular carcinoma.
abstract: Multifunctional theranostic nanoparticles represent an emerging agent with the potential to offer extremely sensitive diagnosis and targeted cancer therapy. Herein, we report the synthesis and characterization of a multifunctional theranostic agent (referred to as LA-LAPNHs) for targeted magnetic resonance imaging/computed X-ray tomography (MRI/CT) dual-mode imaging and photothermal therapy of hepatocellular carcinoma. The LA-LAPNHs were characterized as having a core-shell structure with the gold nanoparticles (AuNPs)@polydopamine (PDA) as the inner core, the indocyanine green (ICG), which is electrostatically absorbed onto the surface of PDA, as the photothermal therapeutic agent, and the lipids modified with gadolinium-1,4,7,10-tetraacetic acid and lactobionic acid (LA), which is self-assembled on the outer surface as the shell. The LA-LAPNHs could be selectively internalized into the hepatocellular cell line (HepG2 cells) but not into HeLa cells due to the specific recognition ability of LA to asialoglycoprotein receptor. Additionally, the dual-mode imaging ability of the LA-LAPNH aqueous solution was confirmed by enhanced MR and CT imaging showing a shorter T1 relaxation time and a higher Hounsfield unit value, respectively. In addition, the LA-LAPNHs showed significant photothermal cytotoxicity against liver cancer cells with near-infrared irradiation due to their strong absorbance in the region between 700 and 850 nm. In summary, this study demonstrates that LA-LAPNHs may be a promising candidate for targeted MR/CT dual-mode imaging and photothermal therapy of hepatocellular carcinoma.
authors.affiliation: Liver Disease Center, The First Affiliated Hospital of Fujian Medical University , Fuzhou 350005, P. R. China.
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Zeng
authors.forename: Da
authors.initials: D
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.forename: Xueyong
authors.initials: X
authors.lastname: Wei
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Liu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000628:therapy*; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D003952:Diagnostic Imaging / Q000379:methods*; D005682:Gadolinium / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008113:Liver Neoplasms / Q000175:diagnosis / Q000628:therapy; D008279:Magnetic Resonance Imaging / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D010789:Phototherapy / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D007211:Indoles; D008055:Lipids; D011108:Polymers; C568283:polydopamine; D006046:Gold; D005682:Gadolinium; D007208:Indocyanine Green