title: AAPE proliposomes for topical atopic dermatitis treatment.
abstract: BACKGROUND
  Anti-inflammatory effect of advanced adipose stem cell derived protein extract (AAPE) could be improved by minimising protein degradation.
  
  
  OBJECTIVE
  To develop a proliposomal formulation of AAPE for the treatment of topical atopic dermatitis.
  
  
  METHODS
  Proliposomal powder was manufactured by evaporating a solution of soy phosphatidyl choline, AAPE and Poloxamer 407 in ethanol under vacuum on sorbitol powder. Characterisation of proliposomes (zeta potential, diameter, stability and flowability) as well as in vivo efficacy in a dermatitis mouse model was investigated.
  
  
  CONCLUSIONS
  Reconstitution of the proliposomal powder formed liposomes of 589 ± 3.6 nm diameter with zeta potential of -51.33 ± 0.36 mV. Protein stability was maintained up to 90 days at 25 °C as proliposomes. In vivo studies on atopic dermatitis mouse model showed a significant reduction in IgE levels after topical AAPE proliposome treatment.
  
  
  CONCLUSIONS
  AAPE proliposomes maintained protein stability and showed promising results for atopic dermatitis treatment.
authors.affiliation: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Seoul National University , Seoul , South Korea and.
authors.forename: Alexander
authors.initials: A
authors.lastname: Jahn
authors.forename: Chung Kil
authors.initials: CK
authors.lastname: Song
authors.forename: Prabagar
authors.initials: P
authors.lastname: Balakrishnan
authors.forename: Soon-Sun
authors.initials: SS
authors.lastname: Hong
authors.forename: Ju-Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Suk-Jae
authors.initials: SJ
authors.lastname: Chung
authors.forename: Dae-Duk
authors.initials: DD
authors.lastname: Kim
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D003876:Dermatitis, Atopic / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D020442:Poloxamer / Q000737:chemistry / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology; D013234:Stem Cells / Q000737:chemistry*
chemical_list: D011506:Proteins; D020442:Poloxamer
keywords: AAPE; ADSC; atopic dermatitis; liposomes; proliposomes