title: Effect of dietary management on the gastric endocrine cells in patients with irritable bowel syndrome.
abstract: OBJECTIVE
  The gastric endocrine cells in patients with irritable bowel syndrome (IBS) tend to normalize following dietary guidance. The aim of the present study was to identify the gastric endocrine cell types that are changed following such dietary guidance.
  
  
  METHODS
  Fourteen IBS patients and 14 healthy subjects were included in the study. Patients received three sessions of individual dietary management guidance. Gastroscopy was performed on both the controls and the patients at baseline and then again for the patients at 3-9 months after dietary guidance. Biopsy samples from the corpus and antrum were immunostained for all gastric endocrine cell types. Endocrine cells were quantified by computerized image analysis.
  
  
  RESULTS
  The densities of the ghrelin cells for the controls and IBS patients before and after dietary guidance were 149.6 ± 36.2 (mean ± s.e.m.; 95% confidence interval (CI) 71.3-227.8), 114.5 ± 32.7 and 161.8 ± 37.8 cells/mm(2), respectively. The densities of the gastrin cells in these groups were 155.8 ± 21.0 (95% CI 110.3-201.2), 159.4 ± 24.3 and 211.6 ± 28.0 cells/mm(2), respectively; the corresponding densities of serotonin cells in the corpus were 18.2 ± 3.9 (95% CI 9.8-26.6), 10.6 ± 3.4 and 14 ± 2.0 cells/mm(2) and in the antrum were 44.6 ± 12.2 (95% CI 18.1-71.1), 1.7 ± 0.5 and 14.7 ± 6.3 cells/mm(2). The densities of the somatostatin cells in the corpus were 40.0 ± 7.7 (95% CI 23.5-56.5), 23.0 ± 3.0 and 37.3 ± 4.2 cells/mm(2), respectively, and in the antrum were 138.9 ± 22.0 (95% CI 91.4-186.3), 95.6 ± 15.9 and 86.0 ± 16.9 cells/mm(2), respectively.
  
  
  CONCLUSIONS
  The densities of all of the gastric endocrine cell types changed towards the healthy control values in the IBS patients following a change in food intake.
authors.affiliation: 1] Section for Gastroenterology, Department of Medicine, Stord Hospital, Stord, Norway [2] Section for Gastroenterology, Institute of Medicine, Bergen University, Bergen, Norway.
authors.forename: T
authors.initials: T
authors.lastname: Mazzawi
authors.affiliation: Section for Gastroenterology, Institute of Medicine, Bergen University, Bergen, Norway.
authors.forename: T
authors.initials: T
authors.lastname: Hausken
authors.affiliation: Department of Research, Helse-Fonna, Haugesund, Norway.
authors.forename: D
authors.initials: D
authors.lastname: Gundersen
authors.affiliation: 1] Section for Gastroenterology, Department of Medicine, Stord Hospital, Stord, Norway [2] Section for Gastroenterology, Institute of Medicine, Bergen University, Bergen, Norway.
authors.forename: M
authors.initials: M
authors.lastname: El-Salhy
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004032:Diet; D055098:Endocrine Cells / Q000166:cytology*; D005260:Female; D005755:Gastrins / Q000378:metabolism; D005773:Gastroscopy; D054439:Ghrelin / Q000378:metabolism; D064368:Healthy Volunteers; D006801:Humans; D007091:Image Processing, Computer-Assisted; D043183:Irritable Bowel Syndrome / Q000178:diet therapy*; D008297:Male; D008875:Middle Aged; D015596:Nutrition Assessment; D018673:Nutrition Policy; D012701:Serotonin / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism; D013270:Stomach / Q000166:cytology*; D055815:Young Adult
chemical_list: D005755:Gastrins; D054439:Ghrelin; D012701:Serotonin; D013004:Somatostatin
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