title: A rapid SNAP-tag fluorogenic probe based on an environment-sensitive fluorophore for no-wash live cell imaging.
abstract: One major limitation of labeling proteins with synthetic fluorophores is the high fluorescence background, which necessitates extensive washing steps to remove unreacted fluorophores. In this paper, we describe a novel fluorogenic probe based on an environment-sensitive fluorophore for labeling with SNAP-tag proteins. The probe exhibits dramatic fluorescence turn-on of 280-fold upon being labeled to SNAP-tag. The major advantages of our fluorogenic probe are the dramatic fluorescence turn-on, ease of synthesis, high selectivity, and rapid labeling with SNAP-tag. No-wash labeling of both intracellular and cell surface proteins was successfully achieved in living cells, and the localization of these proteins was specifically visualized.
authors.affiliation: Department of Chemistry and ‡Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University , 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC).
authors.forename: Tao-Kai
authors.initials: TK
authors.lastname: Liu
authors.forename: Pei-Ying
authors.initials: PY
authors.lastname: Hsieh
authors.forename: Yu-De
authors.initials: YD
authors.lastname: Zhuang
authors.forename: Chi-Yang
authors.initials: CY
authors.lastname: Hsia
authors.forename: Chi-Ling
authors.initials: CL
authors.lastname: Huang
authors.forename: Hsiu-Ping
authors.initials: HP
authors.lastname: Lai
authors.forename: Hung-Sheung
authors.initials: HS
authors.lastname: Lin
authors.forename: I-Chia
authors.initials: IC
authors.lastname: Chen
authors.forename: Hsin-Yun
authors.initials: HY
authors.lastname: Hsu
authors.forename: Kui-Thong
authors.initials: KT
authors.lastname: Tan
mesh_terms: D003600:Cytosol / Q000378:metabolism*; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D045325:HCT116 Cells; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes; D011506:Proteins / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D011506:Proteins