title: Interferon alpha-inducible protein 27 promotes epithelial-mesenchymal transition and induces ovarian tumorigenicity and stemness.
abstract: BACKGROUND
  Interferon alpha-inducible protein 27 (IFI27) is an interferon alpha-inducible protein, which was found to be upregulated in some cancers, such as breast cancer, squamous cell carcinoma, hepatocellular carcinoma, and serous ovarian carcinoma. However, the role of IFI27 in ovarian cancer remains to be elucidated. This study was designed to investigate the role of IFI27 in ovarian cancer tumorigenicity.
  
  
  METHODS
  The expression of IFI27 was examined in ovarian cancer tissues and cell lines by real time quantitative reverse transcription polymerase chain reaction and immunohistochemistry. The cell migration and invasion was investigated by wound healing and transwell invasion assay. The epithelial-mesenchymal transition markers were detected by Western blotting and the stemness was evaluated by sphere formation. The tumor growth was examined in the athymic mice model.
  
  
  RESULTS
  We found that IFI27 is overexpressed in ovarian cancer and associated with patients' survival. Interestingly, we further observed that the expression of IFI27 was associated with the expression of mesenchymal marker vimentin in ovarian cancer. Overexpression of IFI27 induces epithelial-mesenchymal transition and promotes epithelial ovarian cancer cells migration and invasion, tumorigenicity, stemness, and drug resistance. Moreover, overexpression of IFI27 is associated with loss of miR-502 in ovarian cancer. Reexpression of miR-502 inhibits IFI27-induced tumorigenicity, migration, and drug resistance.
  
  
  CONCLUSIONS
  These data suggested that IFI27 may be a potential target for developing novel diagnosis strategies and therapeutic interventions.
authors.affiliation: Department of Obstetrics and Gynecology, Yijishan Hospital, Wannan Medical College, Wuhu, China.
authors.forename: Shuqin
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Yangpu District Central Hospital, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Pathology, Yijishan Hospital, Wannan Medical College, Wuhu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Hefei First People's Hospital, Hefei, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Obstetrics and Gynecology, Yijishan Hospital, Wannan Medical College, Wuhu, China.
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Yijishan Hospital, Wannan Medical College, Wuhu, China.
authors.forename: Guoxuan
authors.initials: G
authors.lastname: Zheng
authors.affiliation: Department of Obstetrics and Gynecology, Yijishan Hospital, Wannan Medical College, Wuhu, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, China. Electronic address: xiahp82@gmail.com.
authors.forename: Hongping
authors.initials: H
authors.lastname: Xia
authors.affiliation: Department of Pathology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China. Electronic address: tutufoliage@hotmail.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Tao
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D063646:Carcinogenesis / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D019008:Drug Resistance, Neoplasm; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000473:pathology*; D014746:Vimentin / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C406887:IFI27 protein, human; D016898:Interferon-alpha; C545730:MIRN502 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D014746:Vimentin
keywords: Epithelial–mesenchymal transition; IFI27; Metastasis; Ovarian cancer; miR-502