title: Mechanical characterization of a bifunctional Tetronic hydrogel adhesive for soft tissues.
abstract: Although a number of tissue adhesives and sealants for surgical use are currently available, attaining a useful balance in high strength, high compliance, and low swelling has proven difficult. Recent studies have demonstrated that a four-arm poly(propylene oxide)-poly(ethylene oxide) block copolymer, Tetronic, can be chemically modified to form a hydrogel tissue adhesive (Cho et al., Acta Biomater 2012;8:2223-2232; Barrett et al., Adv Health Mater 2012;1-11; Balakrishnan, Evaluating mechanical performance of hydrogel-based adhesives for soft tissue applications. Clemson University, All Theses, Paper 1574: Tiger Prints; 2013). Building on the success of these studies, this study explored bifunctionalization of Tetronic with acrylates for chemical crosslinking of the hydrogel and N-hydroxysuccinimide (NHS) for reaction with tissue amines. The adhesive bond strengths of various uni and bifunctional Tetronic blends (T1107 ACR: T1107 ACR/NHS) determined by lap shear testing ranged between 8 and 74 kPa, with the 75:25 (T1107 ACR: T1107 ACR/NHS) blend displaying the highest value. These results indicated that addition of NHS led to improvement of tissue bond strength over acrylation alone. Furthermore, ex vivo pressure tests using the rat bladder demonstrated that the bifunctional Tetronic adhesive exhibited high compliance and maintained pressures under hundreds of filling and emptying cycles. Together, the results of this study provided evidence that the bifunctional Tetronic adhesive with a proper blend ratio may be used to achieve an accurate balance in bulk and tissue bond strengths, as well as the compliance and durability for soft tissue such as the bladder.
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, South Carolina, 29634.
authors.forename: Lindsey
authors.initials: L
authors.lastname: Sanders
authors.forename: Roland
authors.initials: R
authors.lastname: Stone
authors.forename: Kenneth
authors.initials: K
authors.lastname: Webb
authors.forename: Thompson
authors.initials: T
authors.lastname: Mefford
authors.forename: Jiro
authors.initials: J
authors.lastname: Nagatomi
mesh_terms: D000269:Adhesives / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D006358:Hot Temperature; D020100:Hydrogels / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D011108:Polymers / Q000737:chemistry; D011312:Pressure; D051381:Rats; D033081:Shear Strength; D013314:Stress, Mechanical; D013388:Succinimides / Q000737:chemistry
chemical_list: D000269:Adhesives; D000588:Amines; D001672:Biocompatible Materials; D020100:Hydrogels; D011108:Polymers; D013388:Succinimides; C001426:N-hydroxysuccinimide
keywords: functionalization; hydrogel; mechanical characterization; poloxamine; tissue adhesive
references.citation: Transfusion. 2008 Jul;48(7):1502-16
references.pmid: 18422855
references.citation: Acta Biomater. 2012 Jul;8(6):2223-32
references.pmid: 22406506
references.citation: Urology. 2001 Dec;58(6):893-8
references.pmid: 11744453
references.citation: JAMA. 1997 May 21;277(19):1559-60
references.pmid: 9153373
references.citation: J Card Surg. 2001 May-Jun;16(3):255-7
references.pmid: 11824673
references.citation: Ann Thorac Surg. 2005 Jul;80(1):106-10; discussion 110-1
references.pmid: 15975350
references.citation: Urology. 2008 Jan;71(1):3-6
references.pmid: 18242353
references.citation: Neurosurgery. 2005 Jul;57(1 Suppl):146-51; discussion 146-51
references.pmid: 15987581
references.citation: Adv Healthc Mater. 2013 May;2(5):745-55
references.pmid: 23184616
references.citation: ANZ J Surg. 2007 Apr;77(4):209-13
references.pmid: 17388821
references.citation: Macromol Biosci. 2013 Mar;13(3):271-88
references.pmid: 23225776
references.citation: J Urol. 2002 Jul;168(1):23-6
references.pmid: 12050484
references.citation: Vox Sang. 1997;72(3):133-43
references.pmid: 9145483
references.citation: Am J Physiol. 1995 Jul;269(1 Pt 2):R98-103
references.pmid: 7631909
references.citation: J Neurosurg. 2007 Apr;106(4 Suppl):302-4
references.pmid: 17465365
references.citation: J Biomech. 2008 Nov 14;41(15):3260-4
references.pmid: 18986654
references.citation: Urology. 2001 Apr;57(4):806-10
references.pmid: 11306419
references.citation: Biomaterials. 1994 Jan;15(1):63-7
references.pmid: 8161661
references.citation: Am J Surg. 2001 Aug;182(2 Suppl):40S-44S
references.pmid: 11566476
references.citation: Eur J Pharm Biopharm. 2008 Jan;68(1):34-45
references.pmid: 17881200
references.citation: Eur J Surg. 1999 Jan;165(1):61-8
references.pmid: 10069636