title: α-Amylase inhibitory triterpene from Abrus precatorius leaves.
abstract: In the screening experiments for porcine pancreatic α-amylase inhibitors in 18 plants obtained from Indonesia, a potent inhibitory activity was detected in the extract of leaves of Abrus precatorius. The enzyme assay-guided fractionation of the extract led to the isolation of a triterpene ketone, lupenone (1), as a potent α-amylase inhibitor, together with 24-methylenecycloartenone (2) and luteolin (3). The mode of inhibition of compound 1 against porcine pancreatic α-amylase was a mixed inhibition. This is the first report that describes the potent α-amylase inhibitory activity of the low-polar triterpene ketone similar to compound 1. A comparison of the activities of the isolate and related compounds indicated the importance of C-3 ketone and the lupane skeleton in the α-amylase inhibitory activity.
authors.affiliation: Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University , Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Yonemoto
authors.forename: Miyuki
authors.initials: M
authors.lastname: Shimada
authors.forename: Maria D P T
authors.initials: MD
authors.lastname: Gunawan-Puteri
authors.forename: Eisuke
authors.initials: E
authors.lastname: Kato
authors.forename: Jun
authors.initials: J
authors.lastname: Kawabata
mesh_terms: D029912:Abrus / Q000737:chemistry*; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D007700:Kinetics; D015394:Molecular Structure; D055292:Pancreatic alpha-Amylases / Q000037:antagonists & inhibitors* / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D013552:Swine; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014315:Triterpenes; D055292:Pancreatic alpha-Amylases
keywords: Abrus precatorius; triterpene ketone; α-amylase inhibitor