title: Interactive host cells related to Mycoplasma suis α-enolase by yeast two-hybrid analysis.
abstract: Mycoplasma suis belongs to the haemotrophic mycoplasmas, which colonise the red blood cells of a wide range of vertebrates. Adhesion to red blood cells is the crucial step in the unique lifecycle of M. suis. In addition to MSG1 protein, α-enolase is the second adhesion protein of M. suis, and may be involved in the adhesion of M. suis to porcine red blood cells (RBC). To simulate the environment of the RBC, we established the cDNA library of swine peripheral blood mononuclear cells (PBMC). The yeast two-hybrid (Y2H) system was adopted to screen α-enolase interactive proteins in the PBMC line. Alignment with the NCBI database revealed four interactive proteins: beta-actin, 60S ribosomal protein L11, clusterin precursor and endonuclease/reverse transcriptase. However, the M. suis α-enolase interactive proteins in the PBMC cDNA library obtained in the current study provide valuable information about the host cell interactions of the M. suis α-enolase protein.
authors.affiliation: Department of Veterinary Medicine, Agriculture College of Yanbian University, Yanji 133002, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Veterinary Medicine, Agriculture College of Yanbian University, Yanji 133002, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Veterinary Medicine, Agriculture College of Yanbian University, Yanji 133002, China.
authors.forename: Jixu
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Veterinary Medicine, Agriculture College of Yanbian University, Yanji 133002, China.
authors.forename: Shujiang
authors.initials: S
authors.lastname: Xue
authors.affiliation: Department of Veterinary Medicine, Agriculture College of Yanbian University, Yanji 133002, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Veterinary Medicine, Agriculture College of Yanbian University, Yanji 133002, China.
authors.forename: Longzheng
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Veterinary Medicine, Agriculture College of Yanbian University, Yanji 133002, China. Electronic address: shoufazhang@sina.com.
authors.forename: Shoufa
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D051152:Clusterin / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D015723:Gene Library; D007963:Leukocytes, Mononuclear / Q000382:microbiology* / Q000473:pathology / Q000502:physiology; D009174:Mycoplasma / Q000235:genetics / Q000502:physiology*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D010751:Phosphopyruvate Hydratase / Q000235:genetics* / Q000502:physiology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D020798:Two-Hybrid System Techniques
chemical_list: D000199:Actins; D051152:Clusterin; D004269:DNA, Bacterial; D010751:Phosphopyruvate Hydratase
keywords: Interactive cellular protein; Mycoplasma suis; Porcine infections; Yeast two-hybrid; α-Enolase gene