title: The inhibition of fluorescence resonance energy transfer between multicolor quantum dots for rapid and sensitive detection of Staphylococcus aureus.
abstract: In this paper, we constructed the fluorescence resonance energy transfer (FRET) system between two multi-color quantum dots (QDs) of two sizes for rapid and sensitive detection of Staphylococcus aureus. In this system, green-emitting QDs conjugated with rabbit anti-S. aureus antibodies were used as energy donors while orange-emitting QDs conjugated with goat-anti-rabbit IgG were used as energy acceptors to form FRET system. Pre-binding of Staphylococcus aureus (S. aureus) on the donor occupied the binding sites and thus blocked resonance energy transfer between two colors QDs, leading to the quenching fluorescence of the acceptor. The fluorescence of acceptor has a linear calibration graph with the concentrations of S. aureus from 52 to 2.6×10(5) CFU mL(-1). The low detection limit was 10 CFU/mL. It was worth mentioning that the detection method of S. aureus had been applied to the analysis of apple juice and milk samples, which could potentially be developed into a sensor in the further study.
authors.affiliation: National R&D Center for Egg Processing, Food Science and Technology College, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Wang
authors.affiliation: National R&D Center for Egg Processing, Food Science and Technology College, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: National R&D Center for Egg Processing, Food Science and Technology College, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, China.
authors.forename: Meihu
authors.initials: M
authors.lastname: Ma
authors.affiliation: National R&D Center for Egg Processing, Food Science and Technology College, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, China. Electronic address: caizhaoxia@mail.hzau.edu.cn.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000818:Animals; D001628:Beverages / Q000382:microbiology; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D057230:Limit of Detection; D008892:Milk / Q000382:microbiology; D010316:Particle Size; D045663:Quantum Dots / Q000737:chemistry*; D011817:Rabbits; D013211:Staphylococcus aureus / Q000302:isolation & purification*
keywords: Fluorescence resonance energy transfer; Food samples; Quantum dots; Staphylococcus aureus detection