title: Rice prolamin extract ameliorates acute murine colitis by inhibiting nuclear factor-kappa B and modulating intestinal apoptosis and cell proliferation.
abstract: We investigated the impact of rice prolamin extract (RPE) on lipopolysaccharide (LPS)-induced nuclear factor (NF)-κB signalling in intestinal epithelial cells and macrophages, and determined the therapeutic efficacy of RPE in acute murine colitis. The effect of RPE on LPS-induced NF-κB signalling and proinflammatory gene expression was evaluated by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, immunofluorescence and electrophoretic mobility shift assay (EMSA). The in-vivo efficacy of RPE was assessed in mice with 3% dextran sulphate sodium (DSS)-induced colitis. Apoptotic and cellular proliferative activities were evaluated by immunostaining with cleaved caspase-3 and proliferating cell nuclear antigen (PCNA) antibodies. RPE inhibited LPS-induced expression of monocyte chemotactic protein (MCP)-1, interleukin (IL)-6 and tumour necrosis factor (TNF)-alpha and LPS-induced NF-κB signalling in intestinal epithelial cells and macrophages. RPE-fed, DSS-exposed mice showed less weight loss, longer colon length and lower histological score compared to control diet-fed, DSS-exposed mice. Immunostaining analysis revealed a significant decrease of cleaved caspase-3 positive cells in RPE-fed, DSS-exposed mice compared to DSS-exposed mice. Also, the number of PCNA-positive cells within intact colonic crypts decreased significantly in RPE-fed, DSS-exposed mice compared to control diet-fed, DSS-exposed mice. DSS-induced NF-κB signalling was inhibited by RPE. RPE ameliorates intestinal inflammation by inhibiting NF-κB activation and modulating intestinal apoptosis and cell proliferation in an acute murine colitis.
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: C-Y
authors.initials: CY
authors.lastname: Chung
authors.forename: Y-L
authors.initials: YL
authors.lastname: Park
authors.forename: N
authors.initials: N
authors.lastname: Kim
authors.forename: H-H
authors.initials: HH
authors.lastname: Oh
authors.forename: D-S
authors.initials: DS
authors.lastname: Myung
authors.forename: J-S
authors.initials: JS
authors.lastname: Kim
authors.forename: S-B
authors.initials: SB
authors.lastname: Cho
authors.forename: W-S
authors.initials: WS
authors.lastname: Lee
authors.forename: H-S
authors.initials: HS
authors.lastname: Kim
authors.forename: B-W
authors.initials: BW
authors.lastname: Ahn
authors.forename: Y-E
authors.initials: YE
authors.lastname: Joo
mesh_terms: D000208:Acute Disease; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003092:Colitis / Q000188:drug therapy*; D007422:Intestines / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D012275:Oryza / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D055315:Prolamins / Q000494:pharmacology*
chemical_list: D016328:NF-kappa B; D010936:Plant Extracts; D055315:Prolamins
keywords: NF-κB; colon inflammation; dextran sulphate sodium; prolamin; rice
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