title: Expression profiling of bioactive genes from Moringa oleifera.
abstract: Plants are under constant assault by biotic and abiotic agents. When an elicitor is prologued, an immense reprogramming of plant gene expression and defense responses are initiated, which could be a natural source for potential drug development and insertional mutagenesis. In this regard, differential expression analysis of a medicinal plant Moringa oleifera was performed for bioactive genes at seedling stage, using differential display-RT-PCR technique. Infected seedlings with a fungus Fusarium solani collected at different time intervals, showed a massive change in their gene expression profile. The data analysis revealed that at least 150 pathogen-induced and about 60 suppressed genes were differentially expressed at 8-h postinoculation of the biotic stress. Fifty-five selective genes were disunited and reamplified. Sequence analysis of these potential genes illustrated that these genes had properties of some induced peroxidase mRNA, cell proliferation, others were mitogen activated protein kinases, ribosomal protein genes, defense regulating genes, and a few also had structural properties. Further studies about the utility of these genes in plant metabolism could assist to develop improved transgenic breeds with enhanced value of infection tolerance not only of M. oleifera but of other cultivars also.
authors.affiliation: Molecular Biochemistry Lab, Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Raheela
authors.initials: R
authors.lastname: Jabeen
authors.forename: Ghulam
authors.initials: G
authors.lastname: Mustafa
authors.forename: Zain
authors.initials: Z
authors.lastname: Ul Abdin
authors.forename: M Javid
authors.initials: MJ
authors.lastname: Iqbal
authors.forename: Amer
authors.initials: A
authors.lastname: Jamil
mesh_terms: D020869:Gene Expression Profiling; D017343:Genes, Plant; D029588:Moringa oleifera / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction