title: PEI/Zr⁴⁺-coated nanopore for selective and sensitive detection of ATP in combination with single-walled carbon nanotubes.
abstract: By virtue of a biomimetic nanopore and single-walled carbon nanotubes, a new sensor for adenosine triphosphate (ATP) detection is designed. As compared to the routine approach, the present scenario does not entail the surface modification of nanopore with analyte-specific probes. The underlying mechanism relies on a symmetric nanopore sequentially modified with polyethyleneimine (PEI) and Zr(4+) that can quantitate the concentration of ATP-bound aptamer, while other free aptamers are removed by single-walled carbon nanotubes (SWNTs). The detection limit of the nanopore sensor is 27.46 nM, and the linear range is from 50 nM to 400 nM. The biosensor with an excellent selectivity against guanosine triphosphate (GTP), uridine triphosphate (UTP), and cytosine triphosphate (CTP) can be applied in the real samples such as Hela cell.
authors.affiliation: College of Sciences, Northeastern University, Shenyang 110819, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, Jilin 130022, China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, Jilin 130022, China.
authors.forename: Amin
authors.initials: A
authors.lastname: Bao
authors.affiliation: College of Sciences, Northeastern University, Shenyang 110819, China. Electronic address: sun1th@163.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, Jilin 130022, China. Electronic address: ekwang@ciac.jl.cn.
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
authors.affiliation: College of Sciences, Northeastern University, Shenyang 110819, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, Jilin 130022, China. Electronic address: jhwang@ciac.jl.cn.
authors.forename: Jiahai
authors.initials: J
authors.lastname: Wang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006367:HeLa Cells; D006801:Humans; D007097:Imines / Q000737:chemistry*; D058608:Nanopores / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011095:Polyethylenes / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015040:Zirconium / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D020099:Coated Materials, Biocompatible; D007097:Imines; D037742:Nanotubes, Carbon; D011095:Polyethylenes; C505405:poly(ethylene imine); D000255:Adenosine Triphosphate; D015040:Zirconium
keywords: ATP detection; Aptamer; Nanopore; Polyethyleneimine; Single-walled carbon nanotubes; Zr(4+)