title: Hyaluronic acid behavior in the lamina propria of the larynx with advancing age.
abstract: OBJECTIVE
  To analyze the hyaluronic acid behavior in the lamina propria of the larynx with advancing age.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  UNESP-Univ Estadual Paulista, Botucatu Medical School, Brazil.
  
  
  METHODS
  Thirty vocal folds were obtained at necropsy from 10 adult males (30-50 years old) and 20 geriatric males (10: 60-75 years old; 10: over 76 years old). Midmembranous vocal fold sections were subjected to immunohistochemical reactions. Digital imaging software (ImageJ) was used to quantify the hyaluronic acid distribution over the lamina propria of vocal folds, from superficial to deep layers.
  
  
  RESULTS
  Hyaluronic acid distribution was homogeneous for the larynges, at both superficial and deep layers (41.6 and 38.5, respectively). For both 30- to 50-year-old men geriatric age groups, hyaluronic acid level was lower at both layers.
  
  
  CONCLUSIONS
  Hyaluronic acid level in the lamina propria of the larynx of geriatric men decreases with advancing age at both layers of the lamina.
authors.affiliation: Head and Neck Surgery Department, Univ Estadual Paulista, Botucatu Medical School, Ophthalmology, Otorhinolaryngology, São Paulo, Brazil.
authors.forename: Anete
authors.initials: A
authors.lastname: Branco
authors.affiliation: São Paulo State Technological College/FATEC BT, São Paulo, Brazil.
authors.forename: Sergio Augusto
authors.initials: SA
authors.lastname: Rodrigues
authors.affiliation: Department of Pathology, Univ Estadual Paulista, Botucatu Medical School, São Paulo, Brazil.
authors.forename: Alexandre Todorovic
authors.initials: AT
authors.lastname: Fabro
authors.affiliation: Veterinary Clinic Department, School of Veterinary Medicine and Animal Science/UNESP-Univ Estadual Paulista, São Paulo, Brazil.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Fonseca-Alves
authors.affiliation: Head and Neck Surgery Department, Univ Estadual Paulista, Botucatu Medical School, Ophthalmology, Otorhinolaryngology, São Paulo, Brazil rmartins@fmb.unesp.br.
authors.forename: Regina Helena Garcia
authors.initials: RH
authors.lastname: Martins
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism* / Q000473:pathology; D002102:Cadaver; D016022:Case-Control Studies; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D011446:Prospective Studies; D046509:Tissue Culture Techniques; D014827:Vocal Cords / Q000378:metabolism* / Q000473:pathology
chemical_list: D006820:Hyaluronic Acid
keywords: hyaluronic acid; lamina propria; larynx; presbyphonia; vocal folds