title: Sequence analysis and characterization of plasmid pLK39 isolated from endophytic Salmonella sp. isolated from Solanum lycocarpum.
abstract: The complete nucleotide sequence of a small cryptic plasmid pLK39 isolated from endophytic Salmonella sp. was determined. This plasmid is 4,029 bp long with an overall GC content of 55.4 %. Sequence analyses of pLK39 revealed extensive homology to several plasmids: pRK10, pK, pSW200, pBERT, pST728/06-2, pSW100, pEC3, and pUCD5000. Using the ORF Finder program, 35 putative ORFs was identified, 30 showed more than 35 residues. After performing a search for homologous sequences to the pLK39 at BLASTn software on NCBI, it was ascertained that the plasmid has a ColE1-like replication origin and also a region of mobilization proteins from relaxase family (mobCABD). Besides these mobilization proteins, the pLK39 codes a putative DUF903 protein family, which is characterized as assumed external cytoplasmic membrane lipoprotein. A recombinant form of pLK39 carrying a kanamycin resistance gene is stably maintained in Escherichia coli cells grown in the absence of selection pressure. pLK39 was compatible with pUC18, pBR322, and pACYC184.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, 74001-970, Brazil, periclesdourado@yahoo.com.br.
authors.forename: Pericles Lopes
authors.initials: PL
authors.lastname: Dourado
authors.forename: Vera Lúcia
authors.initials: VL
authors.lastname: Cardoso
authors.forename: Alexandre Siqueira Guedes
authors.initials: AS
authors.lastname: Coelho
authors.forename: Luiz Artur Mendes
authors.initials: LA
authors.lastname: Bataus
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D060026:Endophytes / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D018741:Replication Origin; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D032322:Solanum / Q000382:microbiology*
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