title: Prolonged ingestion of ovalbumin diet by sensitized mice improves the metabolic consequences induced by experimental food allergy.
abstract: The prevalence of food allergy is rising in the western world. Allergen restriction is the chosen treatment in this condition, but continuous ingestion of the antigen has shown positive results in clinical trials. In a previous study, we have shown several allergic and metabolic alterations after 7 days of ovalbumin (OVA) ingestion by sensitized mice. The aim of this study was to investigate whether prolonged ingestion of antigen by sensitized mice would reverse the metabolic consequences caused by experimental food allergy. For this, allergic and metabolic parameters were analysed after prolonged ingestion of an OVA diet by OVA-sensitized mice. As shown previously, after 7 days of OVA consumption, sensitized mice showed increased serum levels of anti-OVA immunoglobulin (Ig)E and IgG1, aversion to the antigen ingestion, marked body and adipose tissue weight loss, followed by adipose tissue inflammation and decreased serum levels of adipokines, glucose and triglycerides. However, after 14 days of oral challenge, sensitized mice showed an anti-OVA IgE level similar to the mice that were only sensitized, but the specific IgG1 did not change. With this prolonged ingestion of OVA, sensitized mice were protected from OVA-induced anaphylaxis when the antigen was given systemically at a dose of 2 mg/animal. Moreover, various parameters analysed were significantly ameliorated, including adipose tissue inflammation, body and adipose tissue loss, as well as serum levels of adipokines and triglycerides. Therefore, our data suggest that prolonged ingestion of OVA by sensitized mice results in an improvement of the metabolic consequences caused by experimental food allergy.
authors.affiliation: Department of Biochemistry and Immunology, Biological Sciences Institute, Belo Horizonte, Brazil.
authors.forename: N V
authors.initials: NV
authors.lastname: Batista
authors.forename: R V S
authors.initials: RV
authors.lastname: Pereira
authors.forename: M L M
authors.initials: ML
authors.lastname: Noviello
authors.forename: L P A
authors.initials: LP
authors.lastname: Dourado
authors.forename: D A
authors.initials: DA
authors.lastname: Perez
authors.forename: G
authors.initials: G
authors.lastname: Foureaux
authors.forename: A J
authors.initials: AJ
authors.lastname: Ferreira
authors.forename: A V M
authors.initials: AV
authors.lastname: Ferreira
authors.forename: D C
authors.initials: DC
authors.lastname: Cara
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology; D000707:Anaphylaxis / Q000517:prevention & control; D000821:Animal Feed; D000818:Animals; D005512:Food Hypersensitivity / Q000276:immunology / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D015431:Weight Loss
chemical_list: D007073:Immunoglobulin E; D010047:Ovalbumin; D005947:Glucose
keywords: food allergy; inflammation; metabolism
references.citation: ISRN Allergy. 2011 Dec 01;2011:818239
references.pmid: 23724232
references.citation: Cell Immunol. 2010;262(1):62-8
references.pmid: 20092813
references.citation: Allergy. 2009 Aug;64(8):1218-20
references.pmid: 19226304
references.citation: Immunol Allergy Clin North Am. 2011 May;31(2):367-76, xi
references.pmid: 21530825
references.citation: Arterioscler Thromb Vasc Biol. 2005 Oct;25(10):2062-8
references.pmid: 16123319
references.citation: J Allergy Clin Immunol. 2010 Feb;125(2 Suppl 2):S116-25
references.pmid: 20042231
references.citation: Clin Exp Allergy. 2011 Sep;41(9):1273-81
references.pmid: 21414048
references.citation: J Allergy Clin Immunol. 2012 Jun;129(6):1579-1587.e1
references.pmid: 22554705
references.citation: Cell Immunol. 2013 Jul-Aug;284(1-2):20-8
references.pmid: 23916876
references.citation: J Clin Invest. 2006 Mar;116(3):833-41
references.pmid: 16498503
references.citation: Neuroimmunomodulation. 2009 Jan;16(1):19-27
references.pmid: 19077442
references.citation: Allergy. 2008 Mar;63(3):354-9
references.pmid: 18053008
references.citation: Diabetes. 2002 Jun;51(6):1737-44
references.pmid: 12031960
references.citation: Nature. 2012 Apr 25;484(7395):465-72
references.pmid: 22538607
references.citation: Clin Exp Allergy. 1992 Aug;22(8):762-6
references.pmid: 1525695
references.citation: J Nutr. 1993 Nov;123(11):1923-31
references.pmid: 8229309
references.citation: Curr Opin Allergy Clin Immunol. 2010 Jun;10(3):214-9
references.pmid: 20431369
references.citation: J Biol Chem. 1964 Feb;239:375-80
references.pmid: 14169133
references.citation: Braz J Med Biol Res. 1994 Jun;27(6):1331-41
references.pmid: 7534545
references.citation: J Neuroimmunol. 2003 Jul;140(1-2):69-77
references.pmid: 12864973
references.citation: J Allergy Clin Immunol. 2010 Aug;126(2):375-83
references.pmid: 20624641
references.citation: Intern Med. 2006;45(16):941-6
references.pmid: 16974055
references.citation: J Allergy Clin Immunol. 2008 Jun;121(6):1344-50
references.pmid: 18410959
references.citation: Braz J Med Biol Res. 2004 Jun;37(6):809-16
references.pmid: 15264023
references.citation: Cell Immunol. 2012 Dec;280(2):171-81
references.pmid: 23399844
references.citation: Science. 2004 Feb 20;303(5661):1195-8
references.pmid: 14976316
references.citation: Pediatr Allergy Immunol. 2008 Mar;19(2):188-95
references.pmid: 18257908
references.citation: Mol Nutr Food Res. 2011 Oct;55(10):1475-83
references.pmid: 21714123
references.citation: J Allergy Clin Immunol. 2008 Dec;122(6):1154-60
references.pmid: 18951617
references.citation: Stem Cells. 2010 Nov;28(11):2065-72
references.pmid: 20845475
references.citation: Cell Immunol. 2011;270(2):198-206
references.pmid: 21636080
references.citation: J Allergy Clin Immunol. 2005 Jan;115(1):3-12; quiz 13
references.pmid: 15637539
references.citation: Vitam Horm. 2000;59:265-304
references.pmid: 10714243
references.citation: Immunol Rev. 2005 Aug;206:232-59
references.pmid: 16048553
references.citation: J Allergy Clin Immunol. 2007 Jan;119(1):199-205
references.pmid: 17208602
references.citation: J Hypertens. 2004 Oct;22(10):1977-82
references.pmid: 15361770
references.citation: Curr Opin Allergy Clin Immunol. 2001 Jun;1(3):229-36
references.pmid: 11964694
references.citation: J Clin Endocrinol Metab. 2008 Feb;93(2):543-9
references.pmid: 18029463
references.citation: Nature. 1994 Dec 1;372(6505):425-32
references.pmid: 7984236
references.citation: Diabetes. 2006 Nov;55(11):3083-90
references.pmid: 17065346
references.citation: J Allergy Clin Immunol. 2007 Sep;120(3):638-46
references.pmid: 17628647
references.citation: J Clin Endocrinol Metab. 2003 Jul;88(7):3005-10
references.pmid: 12843134
references.citation: Curr Opin Lipidol. 2003 Dec;14(6):561-6
references.pmid: 14624132
references.citation: Nature. 2001 Jan 18;409(6818):307-12
references.pmid: 11201732