title: The chaperonin CCTα is required for efficient transcription and replication of rabies virus.
abstract: Negri bodies (NBs) are formed in the cytoplasm of rabies virus (RABV)-infected cells and are accompanied by a number of host factors to NBs, in which replication and transcription occur. Here, it was found that chaperonin containing TCP-1 subunit alpha (CCTα) relocalizes to NBs in RABV-infected cells, and that cotransfection of nucleo- and phospho-proteins of RABV is sufficient to recruit CCTα to the NBs' structure. Inhibition of CCTα expression by specific short hairpin RNA knockdown inhibited the replication and transcription of RABV. Therefore, this study showed that the host factor CCTα is associated with RABV infection and is very likely required for efficient virus transcription and replication.
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, 310058; State Key Laboratory and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou, 310003; Research Center of Molecular Medicine of Yunnan Province, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Jinyang
authors.initials: J
authors.lastname: Zhang
authors.forename: Chengjin
authors.initials: C
authors.lastname: Ye
authors.forename: Xizhen
authors.initials: X
authors.lastname: Ruan
authors.forename: Jie
authors.initials: J
authors.lastname: Zan
authors.forename: Yunbin
authors.initials: Y
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Liao
authors.forename: Jiyong
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002460:Cell Line; D056404:Chaperonin Containing TCP-1 / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D007181:Inclusion Bodies, Viral / Q000201:enzymology / Q000821:virology; D051379:Mice; D021381:Protein Transport; D011818:Rabies / Q000201:enzymology* / Q000235:genetics / Q000821:virology*; D011820:Rabies virus / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D056404:Chaperonin Containing TCP-1
keywords: Negri body; chaperonin; chaperonin containing TCP-1 subunit alpha; rabies virus