title: Subtype-dependent postnatal development of direction- and orientation-selective retinal ganglion cells in mice.
abstract: The direction-selective ganglion cells (DSGCs) and orientation-selective ganglion cells (OSGCs) encode the directional and the orientational information of a moving object, respectively. It is unclear how DSGCs and OSGCs mature in the mouse retina during postnatal development. Here we investigated the development of DSGCs and OSGCs after eye-opening. We show that 1) DSGCs and OSGCs are present at postnatal day 12 (P12), just before eye-opening; 2) the fractions of both DSGCs and OSGCs increase from P12 to P30; 3) the development of DSGCs and OSGCs is subtype dependent; and 4) direction and orientation selectivity are two separate features of retinal ganglion cells (RGCs) in the mouse retina. We classified RGCs into different functional subtypes based on their light response properties. Compared with P12, the direction and orientation selectivity of ON-OFF RGCs but not ON RGCs became stronger at P30. The tuning width of DSGCs for both ON and ON-OFF subtypes decreased with age. For OSGCs, we divided them into non-direction-selective (non-DS) OSGCs and direction-selective OSGCs (DS&OSGCs). For DS&OSGCs, we found that there was no correlation between the direction and orientation selectivity, and that the tuning width of both ON and ON-OFF subtypes remained unchanged with age. For non-DS OSGCs, the tuning width of ON but not ON-OFF subtype decreased with development. These findings provide a foundation to reveal the molecular and synaptic mechanisms underlying the development of the direction and orientation selectivity in the retina.
authors.affiliation: Department of Ophthalmology, Northwestern University, Evanston, Illinois;
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, Northwestern University, Evanston, Illinois; Department of Neurobiology, Northwestern University, Evanston, Illinois; xiaorong-liu@northwestern.edu.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology and Visual Science, University of Utah, Salt Lake City, Utah.
authors.forename: Ning
authors.initials: N
authors.lastname: Tian
mesh_terms: D000200:Action Potentials; D000818:Animals; D005260:Female; D006128:Growth; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012165:Retinal Ganglion Cells / Q000145:classification* / Q000502:physiology; D012684:Sensory Thresholds
keywords: direction-selective ganglion cell; multielectrode array; orientation-selective ganglion cell; retinal ganglion cell
references.citation: Nature. 2008 Mar 27;452(7186):478-82
references.pmid: 18368118
references.citation: J Physiol. 1968 Dec;199(3):613-35
references.pmid: 5710424
references.citation: Neuron. 2012 Nov 8;76(3):518-25
references.pmid: 23141064
references.citation: J Neurosci. 2011 Jun 15;31(24):8760-9
references.pmid: 21677160
references.citation: Trends Neurosci. 2011 Dec;34(12):638-45
references.pmid: 21872944
references.citation: J Comp Neurol. 2007 Jul 10;503(2):244-59
references.pmid: 17492624
references.citation: J Physiol. 2009 Feb 15;587(Pt 4):819-28
references.pmid: 19103682
references.citation: J Neurosci. 2010 Nov 17;30(46):15664-76
references.pmid: 21084622
references.citation: J Comp Neurol. 2009 Feb 10;512(5):664-87
references.pmid: 19051243
references.citation: PLoS Comput Biol. 2010 Oct 21;6(10):e1000967
references.pmid: 20975932
references.citation: Nat Rev Neurosci. 2012 Feb 08;13(3):194-208
references.pmid: 22314444
references.citation: J Neurophysiol. 1994 May;71(5):1672-91
references.pmid: 8064341
references.citation: J Neurosci. 2013 Jun 26;33(26):10616-24
references.pmid: 23804085
references.citation: J Physiol. 1982 Aug;329:243-61
references.pmid: 7143249
references.citation: Neuron. 2010 Dec 22;68(6):1159-72
references.pmid: 21172616
references.citation: Neuron. 2003 Jul 3;39(1):85-96
references.pmid: 12848934
references.citation: Science. 1963 Feb 1;139(3553):412-4
references.pmid: 13966712
references.citation: J Neurosci. 2013 Jul 31;33(31):12751-63
references.pmid: 23904611
references.citation: Vision Res. 2008 Oct;48(23-24):2466-75
references.pmid: 18782584
references.citation: Neuron. 2008 May 22;58(4):499-506
references.pmid: 18498732
references.citation: J Physiol. 1962 Jan;160:106-54
references.pmid: 14449617
references.citation: Science. 1967 Feb 17;155(3764):841-2
references.pmid: 6019094
references.citation: J Neurosci. 2013 Mar 13;33(11):4642-56
references.pmid: 23486939
references.citation: Nature. 2011 Mar 10;471(7337):183-8
references.pmid: 21390125
references.citation: Neuron. 2003 Jun 5;38(5):689-99
references.pmid: 12797955
references.citation: Neuron. 2012 Nov 21;76(4):713-20
references.pmid: 23177957
references.citation: Nature. 2002 Nov 28;420(6914):411-4
references.pmid: 12459782
references.citation: J Neurophysiol. 2011 Aug;106(2):895-904
references.pmid: 21613583
references.citation: Nature. 1997 Sep 25;389(6649):378-82
references.pmid: 9311778
references.citation: J Comp Neurol. 2002 Sep 16;451(2):115-26
references.pmid: 12209831
references.citation: Neuron. 2011 Aug 11;71(3):425-32
references.pmid: 21835340
references.citation: Nature. 2014 Mar 20;507(7492):358-61
references.pmid: 24572358
references.citation: Vision Res. 2002 Mar;42(6):683-94
references.pmid: 11888534
references.citation: Vis Neurosci. 1998 May-Jun;15(3):439-47
references.pmid: 9685197
references.citation: PLoS One. 2011 May 05;6(5):e19477
references.pmid: 21573161
references.citation: Nature. 2011 Jan 20;469(7330):402-6
references.pmid: 21131947
references.citation: J Physiol. 1967 Feb;188(3):285-307
references.pmid: 6032202
references.citation: Curr Opin Neurobiol. 1997 Aug;7(4):514-22
references.pmid: 9287203
references.citation: Annu Rev Neurosci. 2000;23:441-71
references.pmid: 10845071
references.citation: Invest Ophthalmol Vis Sci. 2013 Feb 07;54(2):1106-17
references.pmid: 23322576
references.citation: J Neurosci. 2002 Apr 1;22(7):2737-47
references.pmid: 11923439
references.citation: Nature. 2001 Jun 7;411(6838):698-701
references.pmid: 11395773
references.citation: Neuron. 2009 May 14;62(3):327-34
references.pmid: 19447089