title: Factors associated with the seroprevalence of leishmaniasis in dogs living around Atlantic Forest fragments.
abstract: Canine visceral leishmaniasis is an important zoonosis in Brazil. However, infection patterns are unknown in some scenarios such as rural settlements around Atlantic Forest fragments. Additionally, controversy remains over risk factors, and most identified patterns of infection in dogs have been found in urban areas. We conducted a cross-sectional epidemiological survey to assess the prevalence of leishmaniasis in dogs through three different serological tests, and interviews with owners to assess features of dogs and households around five Atlantic Forest remnants in southeastern Brazil. We used Generalized Linear Mixed Models and Chi-square tests to detect associations between prevalence and variables that might influence Leishmania infection, and a nearest neighbor dispersion analysis to assess clustering in the spatial distribution of seropositive dogs. Our findings showed an average prevalence of 20% (ranging from 10 to 32%) in dogs. Nearly 40% (ranging from 22 to 55%) of households had at least one seropositive dog. Some individual traits of dogs (height, sterilization, long fur, age class) were found to positively influence the prevalence, while some had negative influence (weight, body score, presence of ectoparasites). Environmental and management features (number of cats in the households, dogs with free-ranging behavior) also entered models as negative associations with seropositivity. Strong and consistent negative (protective) influences of the presence of chickens and pigs in dog seropositivity were detected. Spatial clustering of cases was detected in only one of the five study sites. The results showed that different risk factors than those found in urban areas may drive the prevalence of canine leishmaniasis in farm/forest interfaces, and that humans and wildlife risk infection in these areas. Domestic dog population limitation by gonadectomy, legal restriction of dog numbers per household and owner education are of the greatest importance for the control of visceral leishmaniasis in rural zones near forest fragments.
authors.affiliation: Postgraduate program in Applied Ecology, Department of Biology, Federal University of Lavras, Lavras, Brazil.
authors.forename: Nelson Henrique de Almeida
authors.initials: NH
authors.lastname: Curi
authors.affiliation: Postgraduate program in Ecology, Conservation and Management of Wildlife, Department of Biology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Ana Maria de Oliveira
authors.initials: AM
authors.lastname: Paschoal
authors.affiliation: Postgraduate program in Ecology, Conservation and Management of Wildlife, Department of Biology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Rodrigo Lima
authors.initials: RL
authors.lastname: Massara
authors.affiliation: Laboratory of Leishmaniasis, Ezequiel Dias Foundation-FUNED, Belo Horizonte, Brazil.
authors.forename: Andreza Pain
authors.initials: AP
authors.lastname: Marcelino
authors.affiliation: Laboratory of Leishmaniasis, Ezequiel Dias Foundation-FUNED, Belo Horizonte, Brazil.
authors.forename: Adriana Aparecida
authors.initials: AA
authors.lastname: Ribeiro
authors.affiliation: Postgraduate program in Applied Ecology, Department of Biology, Federal University of Lavras, Lavras, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Passamani
authors.affiliation: Postgraduate program in Applied Ecology, Department of Biology, Federal University of Lavras, Lavras, Brazil.
authors.forename: Guilherme Ramos
authors.initials: GR
authors.lastname: Demétrio
authors.affiliation: Department of Biology, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Adriano Garcia
authors.initials: AG
authors.lastname: Chiarello
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001938:Brazil / Q000453:epidemiology; D002415:Cats; D002645:Chickens; D003430:Cross-Sectional Studies; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology / Q000635:transmission; D004285:Dogs; D062665:Epidemiological Monitoring; D005191:Family Characteristics; D005260:Female; D065928:Forests; D006801:Humans; D018314:Leishmania infantum / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology* / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D016014:Linear Models; D008297:Male; D015995:Prevalence; D012307:Risk Factors; D012424:Rural Population; D016036:Seroepidemiologic Studies; D013552:Swine
chemical_list: D000913:Antibodies, Protozoan
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