title: PVP formulated fullerene (C60) increases Rho-kinase dependent vascular tissue contractility in pregnant Sprague Dawley rats.
abstract: Pregnancy is a unique physiological state, in which C60 fullerene is reported to be distributed in both maternal and fetal tissues. Tissue distribution of C60 differs between pregnant and non-pregnant states, presumably due to functional changes in vasculature during pregnancy. We hypothesized that polyvinylpyrrolidone (PVP) formulated C60 (C60/PVP) increases vascular tissue contractility during pregnancy by increasing Rho-kinase activity. C60/PVP was administered intravenously to pregnant and non-pregnant female Sprague Dawley rats. Vascular responses were assessed using wire myography 24h post-exposure. Increased stress generation was observed in uterine artery, thoracic aorta and umbilical vein. Rho-Rho-kinase mediated force maintenance was increased in arterial segments from C60/PVP exposed pregnant rats when compared to PVP exposed rats. Our findings suggest that intravenous exposure to C60/PVP during pregnancy increases vascular tissue contractility of the uterine artery through elements of Rho-Rho-kinase signaling during late stages of pregnancy.
authors.affiliation: Department of Physiology, Brody School of Medicine, East Carolina University, 600, Moye Boulevard, Greenville, NC 27834, USA.
authors.forename: Achini K
authors.initials: AK
authors.lastname: Vidanapathirana
authors.affiliation: Department of Physiology, Brody School of Medicine, East Carolina University, 600, Moye Boulevard, Greenville, NC 27834, USA.
authors.forename: Leslie C
authors.initials: LC
authors.lastname: Thompson
authors.affiliation: Department of Physiology, Brody School of Medicine, East Carolina University, 600, Moye Boulevard, Greenville, NC 27834, USA.
authors.forename: Erin E
authors.initials: EE
authors.lastname: Mann
authors.affiliation: Department of Physiology, Brody School of Medicine, East Carolina University, 600, Moye Boulevard, Greenville, NC 27834, USA.
authors.forename: Jillian T
authors.initials: JT
authors.lastname: Odom
authors.affiliation: Department of Physiology, Brody School of Medicine, East Carolina University, 600, Moye Boulevard, Greenville, NC 27834, USA.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Holland
authors.affiliation: Discovery Sciences, RTI International, Research Triangle Park, NC 27709, USA.
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Sumner
authors.affiliation: Discovery Sciences, RTI International, Research Triangle Park, NC 27709, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.affiliation: Discovery Sciences, RTI International, Research Triangle Park, NC 27709, USA.
authors.forename: Anita H
authors.initials: AH
authors.lastname: Lewin
authors.affiliation: Discovery Sciences, RTI International, Research Triangle Park, NC 27709, USA.
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Fennell
authors.affiliation: Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, CO 80045, USA.
authors.forename: Jared M
authors.initials: JM
authors.lastname: Brown
authors.affiliation: Department of Physiology, Brody School of Medicine, East Carolina University, 600, Moye Boulevard, Greenville, NC 27834, USA. Electronic address: wingardc@ecu.edu.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Wingard
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004452:Echocardiography; D005260:Female; D037741:Fullerenes / Q000633:toxicity*; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009213:Myography; D011205:Povidone / Q000378:metabolism; D011247:Pregnancy; D017207:Rats, Sprague-Dawley; D054460:rho-Associated Kinases / Q000187:drug effects* / Q000502:physiology
chemical_list: D037741:Fullerenes; D054460:rho-Associated Kinases; D011205:Povidone
keywords: Nanotoxicology; Polyvinylpyrrolidone; Pregnancy; Rho-kinase pathway; Umbilical vein; Uterine artery; Vascular tissue contractility
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