title: The effect of proinflammatory cytokines on IL-17RA expression in NSCLC.
abstract: Interleukin-17 receptor (IL-17RA) is essential for proinflammatory cytokine IL-17-mediated pathogenesis of various tumors. IL-17RA is upregulated by some proinflammatory cytokines such as IL-21 and IL-15 and downregulated by IL-2, while the effect of IL-1β, IL-6, IL-8, TNF-α on IL-17RA expression in non-small cell lung caner (NSCLC) remains unknown. Our findings revealed that IL-17RA mRNA was increased in NSCLC tissues compared with the corresponding peritumor tissues (P = 0.0039) and high expression of IL-17RA protein in human NSCLC tissues was significantly associated with histological subtype, primary tumor size and clinical stages (P = 0.033, 0.033 and 0.011, respectively). IL-17RA mRNA expression was positively related to IL-1β, IL-6, IL-8, TNF-α mRNA expression (P = 0.013, 0.0001, 0.002 and 0.010 respectively) in NSCLC tissues. Furthermore, IL-1β, IL-6, IL-8, TNF-α upregulated IL-17RA mRNA and protein in A549 and H460 cells (all P < 0.05). It is suggested that IL-1β, IL-6, IL-8, TNF-α promoted IL-17RA expression in NSCLC and they may involve in IL-17RA signaling in NSCLC.
authors.affiliation: Department of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, 430022, Hubei, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.forename: Jiao
authors.initials: J
authors.lastname: Du
authors.forename: Jinshuo
authors.initials: J
authors.lastname: Fan
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Lv
authors.forename: Xin
authors.initials: X
authors.lastname: Qian
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Zhang
authors.forename: Jieli
authors.initials: J
authors.lastname: Han
authors.forename: Caiyun
authors.initials: C
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry / Q000453:epidemiology / Q000378:metabolism*; D016207:Cytokines / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008168:Lung / Q000737:chemistry; D008175:Lung Neoplasms / Q000737:chemistry / Q000453:epidemiology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D053722:Receptors, Interleukin-17 / Q000032:analysis / Q000378:metabolism*
chemical_list: D016207:Cytokines; D053722:Receptors, Interleukin-17
references.citation: J Leukoc Biol. 2002 Jan;71(1):1-8
references.pmid: 11781375
references.citation: Nat Immunol. 2005 Nov;6(11):1133-41
references.pmid: 16200068
references.citation: Chest. 2002 Sep;122(3):1037-57
references.pmid: 12226051
references.citation: J Cancer Res Clin Oncol. 2007 Dec;133(12):1017-21
references.pmid: 17690908
references.citation: J Clin Immunol. 1997 Sep;17(5):366-9
references.pmid: 9327335
references.citation: Carcinogenesis. 2009 Jul;30(7):1073-81
references.pmid: 19468060
references.citation: Clin Exp Immunol. 2002 Mar;127(3):539-46
references.pmid: 11966773
references.citation: J Immunol. 2005 Nov 1;175(9):6177-89
references.pmid: 16237115
references.citation: J Biol Chem. 2006 Aug 25;281(34):24138-48
references.pmid: 16798734
references.citation: Int J Cancer. 2007 Dec 1;121(11):2373-80
references.pmid: 17893866
references.citation: Cancer Sci. 2010 Nov;101(11):2384-90
references.pmid: 20825419
references.citation: Expert Opin Ther Targets. 2011 Sep;15(9):1127-37
references.pmid: 21751938
references.citation: Nat Immunol. 2005 Nov;6(11):1123-32
references.pmid: 16200070
references.citation: Am J Respir Cell Mol Biol. 2001 Sep;25(3):335-40
references.pmid: 11588011
references.citation: Immunity. 2006 Feb;24(2):179-89
references.pmid: 16473830
references.citation: J Biol Chem. 2008 May 16;283(20):14100-8
references.pmid: 18348982
references.citation: Am J Physiol Lung Cell Mol Physiol. 2005 Sep;289(3):L446-53
references.pmid: 15894558
references.citation: Curr Opin Genet Dev. 2011 Feb;21(1):80-5
references.pmid: 21144738
references.citation: Lung Cancer. 2010 Sep;69(3):348-54
references.pmid: 20022135
references.citation: Cancer Res. 2014 Jan 1;74(1):8-14
references.pmid: 24362915
references.citation: Cytokine Growth Factor Rev. 2003 Apr;14(2):155-74
references.pmid: 12651226
references.citation: Cancer Res. 2001 May 15;61(10):3986-97
references.pmid: 11358816
references.citation: Immunity. 1995 Dec;3(6):811-21
references.pmid: 8777726
references.citation: Clin Exp Immunol. 2003 Sep;133(3):344-9
references.pmid: 12930359
references.citation: Expert Rev Anticancer Ther. 2008 Apr;8(4):605-15
references.pmid: 18402527
references.citation: Zhonghua Zhong Liu Za Zhi. 2012 Aug;34(8):596-9
references.pmid: 23158993
references.citation: J Cell Physiol. 2010 Aug;224(2):454-64
references.pmid: 20333651
references.citation: Cell Signal. 2011 Jul;23(7):1069-75
references.pmid: 21130872
references.citation: Zhongguo Fei Ai Za Zhi. 2013 Sep;16(9):476-81
references.pmid: 24034995
references.citation: Clin Cancer Res. 2008 Nov 1;14(21):6735-41
references.pmid: 18980965
references.citation: PLoS One. 2011;6(9):e24856
references.pmid: 21949768
references.citation: J Cardiothorac Surg. 2012 Oct 04;7:104
references.pmid: 23034167
references.citation: Curr Opin Immunol. 2007 Jun;19(3):281-6
references.pmid: 17433650
references.citation: J Exp Med. 2005 Jan 3;201(1):139-48
references.pmid: 15630141
references.citation: Cell. 2010 Mar 19;140(6):883-99
references.pmid: 20303878
references.citation: CA Cancer J Clin. 2014 Jan-Feb;64(1):9-29
references.pmid: 24399786
references.citation: Cytokine. 2014 Mar;66(1):23-9
references.pmid: 24548421
references.citation: Immunol Lett. 2012 Dec 17;148(2):144-50
references.pmid: 23089548
references.citation: J Immunol. 2011 Mar 15;186(6):3462-71
references.pmid: 21300825
references.citation: J Immunol. 2010 Nov 15;185(10):6348-54
references.pmid: 20952674
references.citation: Biochem Pharmacol. 2007 Jan 15;73(2):215-27
references.pmid: 17067555