title: miR-129-1-3p promote BGC-823 cell proliferation by targeting PDCD2.
abstract: MicroRNAs (miRNAs) are the class of small noncoding RNAs, and play an important role in the regulation of gene expression at the posttranscriptional level. In this study, we explored the effect of miR-129-1-3p on the growth and cell cycle of human gastric cancer cell line BGC-823. The miR-129-1-3p mimics or inhibitors were transfected into the BGC-823 cell line, and the cell cycle and cell growth was measured by flow cytometry and real-time cell analyzer, respectively. The possible targets of miR-129-1-3p were analyzed by quantitative real time-PCR (QRT-PCR), Western blotting and Luciferase reporter assay. The results showed that miR-129-1-3p could promote the growth and cell cycle of BGC-823 cells. Although protein expression of programmed cell death 2 (PDCD2) was not changed with miR-129-1-3p, QRT-PCR showed that expression of PDCD2 mRNA was negatively related to the miR-129-1-3p. Luciferase reporter assay revealed that PDCD2 is one of the targets of miR-129-1-3p. Our results indicated that miR-129-1-3p might promote proliferation of BGC-823 cells by targeting PDCD2.
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, Medical School of Ningbo University, Zhejiang, China; Ningbo Institute of Medical Sciences, Medical School of Ningbo University, Ningbo, Zhejiang, China.
authors.forename: Yantao
authors.initials: Y
authors.lastname: Du
authors.forename: Danping
authors.initials: D
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Luo
authors.forename: Junming
authors.initials: J
authors.lastname: Guo
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D051017:Apoptosis Regulatory Proteins; D035683:MicroRNAs; C541915:Mirn129 microRNA, human; C094601:PDCD2 protein, human; D012333:RNA, Messenger; D008156:Luciferases
keywords: cell cycle; cell growth; gastric carcinoma; miRNA