title: Direct common gram-negative bacterial identification from positive blood culture bottles by SELDI-TOF MS.
abstract: A protein database was constructed and validated with identification rate over 90% for the 4 most common Gram-negative bacteria on agar plates. By protein masses comparison, 120 bacteria of the 4 species from blood culture bottles were identified. The concordance was high (Kappa=0.906) between our method and conventional approach.
authors.affiliation: Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Daiwen
authors.initials: D
authors.lastname: Xiao
authors.affiliation: Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Hangfeng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.affiliation: Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yu
authors.affiliation: Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Chunbao
authors.initials: C
authors.lastname: Xie
authors.affiliation: Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhong
authors.affiliation: Clinical Medicine Department, Luzhou Medical College, Luzhou 646000, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Clinical Laboratory Department, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, China. Electronic address: huangwf2002@21cn.com.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Huang
mesh_terms: D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: Bloodstream infection; Identification; Protein database; SELDI-TOF MS