title: Extracellular virion proteins of two Chinese CyHV-3/KHV isolates, and identification of two novel envelope proteins.
abstract: Proteins in extracellular virions of two Chinese cyprinid herpesvirus-3/koi herpesvirus (CyHV-3/KHV) isolates were identified through one-dimensional gel-based matrix-assisted laser desorption/ionization tandem time of flight mass spectrometry in combination with liquid chromatography tandem mass spectrometry (LC ESI-MS/MS). A total of 43 viral proteins were identified, seven of which (pORF62, 68, 43, 51, 92, 84, and 72) were characterized as marked characteristic viral proteins in CyHV-3/KHV virions and six of which (pORF11, 27, 83, 91, 106 and 116) were not detected previously. Of the newly identified proteins, pORF83 was identified as a novel viral minor envelope protein by Western blot analysis and indirect immunofluorescence assay. Another 27 cellular proteins were validated by LC ESI-MS/MS to be involved in purified extracellular CyHV-3/KHV virions, among which a highly abundant, virus-inducible stress protein was shown and characterized as a cell-derived envelope protein in CyHV-3/KHV virions. Our study extends the number of known CyHV-3/KHV virion-associated viral proteins from 40 to 46 and identifies a novel viral envelope protein as well as a cellular envelope protein. The present findings provide some new insights for better understanding CyHV-3/KHV.
authors.affiliation: MOE Key Laboratory of Aquatic Food Safety/State Key Laboratory for Bio-control, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yi
authors.affiliation: MOE Key Laboratory of Aquatic Food Safety/State Key Laboratory for Bio-control, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Hetong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Aquatic Food Safety/State Key Laboratory for Bio-control, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Xuezhu
authors.initials: X
authors.lastname: Lee
authors.affiliation: MOE Key Laboratory of Aquatic Food Safety/State Key Laboratory for Bio-control, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Weng
authors.affiliation: MOE Key Laboratory of Aquatic Food Safety/State Key Laboratory for Bio-control, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China; School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: He
authors.affiliation: MOE Key Laboratory of Aquatic Food Safety/State Key Laboratory for Bio-control, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China. Electronic address: dongchfu@mail.sysu.edu.cn.
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Dong
mesh_terms: D000818:Animals; D002347:Carps / Q000821:virology; D005393:Fish Diseases / Q000821:virology*; D006564:Herpesviridae / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014771:Virion / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D014759:Viral Envelope Proteins
keywords: Cyprinid herpesvirus 3; Envelope proteins; Koi herpesvirus; Structural protein; Virus inducible stress protein