title: Microfluidic-integrated laser-controlled microactuators with on-chip microscopy imaging functionality.
abstract: The fabrication of a novel microfluidic system, integrated with a set of laser-controlled microactuators on an ePetri on-chip microscopy platform, is presented in this paper. In the fully integrated microfluidic system, a set of novel thermally actuated paraffin-based microactuators, precisely controlled by programmed laser optics, was developed to regulate flow and to provide pumping of liquid solutions without external connections. The microfluidic chip was fabricated on a complementary metal-oxide-semiconductor (CMOS)-imaging sensor chip on an ePetri platform; this configuration provided real-time, wide field-of-view, high-resolution imaging using a sub-pixel sweeping microscopy technique. The system of microactuators, which consisted of microvalves and a micropump, operated well in the microfluidic channel with a focused near-infrared laser beam providing the actuation control. As a demonstration, we used our prototype to assess cell-drug interactions and to monitor cell growth directly within an incubator in real time. The powerful combination of laser-actuated microfluidics and chip-scale microscopy techniques represents a significant step forward in terms of a simple, robust, high-throughput, and highly compact analysis system for biomedical and bioscience applications.
authors.affiliation: Department of Electrical Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA.
authors.forename: Jae Hee
authors.initials: JH
authors.lastname: Jung
authors.forename: Chao
authors.initials: C
authors.lastname: Han
authors.forename: Seung Ah
authors.initials: SA
authors.lastname: Lee
authors.forename: Jinho
authors.initials: J
authors.lastname: Kim
authors.forename: Changhuei
authors.initials: C
authors.lastname: Yang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002166:Camptothecin / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D003584:Cytological Techniques / Q000295:instrumentation; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*
chemical_list: D000970:Antineoplastic Agents; D002166:Camptothecin
references.citation: Opt Lett. 2009 Jul 1;34(13):1976-8
references.pmid: 19571971
references.citation: Chem Rev. 1973 Aug;73(4):385-405
references.pmid: 4578778
references.citation: Nucleic Acids Res. 2000 Jun 15;28(12):E60
references.pmid: 10871383
references.citation: Lab Chip. 2007 May;7(5):557-64
references.pmid: 17476373
references.citation: Anal Chem. 2012 Mar 6;84(5):2133-40
references.pmid: 22277086
references.citation: Lab Chip. 2008 Jul;8(7):1198-204
references.pmid: 18584098
references.citation: Lab Chip. 2011 Jan 7;11(1):104-14
references.pmid: 21060932
references.citation: Anal Chem. 2004 Jul 1;76(13):3740-8
references.pmid: 15228349
references.citation: Biotechnol Adv. 2007 Sep-Oct;25(5):483-514
references.pmid: 17601695
references.citation: Clin Cancer Res. 2002 Mar;8(3):641-61
references.pmid: 11895891
references.citation: Soft Matter. 2007 Sep 19;3(10):1223-1230
references.pmid: 32900089
references.citation: Lab Chip. 2011 Jan 7;11(1):70-8
references.pmid: 21042620
references.citation: Lab Chip. 2011 Feb 21;11(4):723-6
references.pmid: 21103528
references.citation: Nature. 2006 Jul 27;442(7101):381-6
references.pmid: 16871205
references.citation: PLoS One. 2014 Feb 26;9(2):e89712
references.pmid: 24586978
references.citation: Anal Chem. 2001 Feb 1;73(3):565-70
references.pmid: 11217764
references.citation: Science. 2000 Apr 7;288(5463):113-6
references.pmid: 10753110
references.citation: J Biol Chem. 1985 Nov 25;260(27):14873-8
references.pmid: 2997227
references.citation: Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):16889-94
references.pmid: 21969539
references.citation: Nature. 2006 Jul 27;442(7101):374-80
references.pmid: 16871204
references.citation: Lab Chip. 2009 May 7;9(9):1254-60
references.pmid: 19370245
references.citation: Anal Chem. 2006 Feb 1;78(3):695-701
references.pmid: 16448041
references.citation: Lab Chip. 2009 Jul 21;9(14):2026-35
references.pmid: 19568671
references.citation: Anal Chem. 2013 Feb 19;85(4):2356-60
references.pmid: 23350531
references.citation: Nature. 2006 Jul 27;442(7101):368-73
references.pmid: 16871203
references.citation: Lab Chip. 2010 Apr 21;10(8):939-56
references.pmid: 20358102
references.citation: Anal Chem. 1998 Dec 1;70(23):4974-84
references.pmid: 21644679
references.citation: Anal Chem. 2004 Apr 1;76(7):1824-31
references.pmid: 15053639
references.citation: Lab Chip. 2007 May;7(5):565-73
references.pmid: 17476374
references.citation: Lab Chip. 2009 Jun 7;9(11):1548-55
references.pmid: 19458861