title: Polymorphism, Expression of Natural Resistance-associated Macrophage Protein 1 Encoding Gene (NRAMP1) and Its Association with Immune Traits in Pigs.
abstract: Natural resistance-associated macrophage protein 1 encoding gene (NRAMP1) plays an important role in immune response against intracellular pathogens. To evaluate the effects of NRAMP1 gene on immune capacity in pigs, tissue expression of NRAMP1 mRNA was observed by real time quantitative polymerase chain reaction (PCR), and the results revealed NRAMP1 expressed widely in nine tissues. One single nucleotide polymorphism (SNP) (ENSSSCG00000025058: g.130 C>T) in exon1 and one SNP (ENSSSCG00000025058: g.657 A>G) in intron1 region of porcine NRAMP1 gene were demonstrated by DNA sequencing and PCR-RFLP analysis. A further analysis of SNP genotypes associated with immune traits including contain of white blood cell (WBC), granulocyte, lymphocyte, monocyte (MO), rate of cytotoxin in monocyte (MC) and CD4/CD8 T lymphocyte subpopulations in blood was carried out in four pig populations including Large White and three Chinese indigenous breeds (Wannan Black, Huai pig and Wei pig). The results showed that the SNP (ENSSSCG00000025058: g.130 C>T) was significantly associated with level of WBC % (p = 0.031), MO% (p = 0.024), MC% (p = 0.013) and CD4(-)CD8(+) T lymphocyte (p = 0.023). The other SNP (ENSSSCG00000025058: g.657 A>G) was significantly associated with the level of MO% (p = 0.012), MC% (p = 0.019) and CD4(-)CD8(+) T lymphocyte (p = 0.037). These results indicate that the NRAMP1 gene can be regarded as a molecular marker for genetic selection of disease susceptibility in pig breeding.
authors.affiliation: Anhui Antai Pig Breeding Co., LTD., Hefei 340100, China .
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Ding
authors.affiliation: Anhui Antai Pig Breeding Co., LTD., Hefei 340100, China .
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Anhui Antai Pig Breeding Co., LTD., Hefei 340100, China .
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Anhui Antai Pig Breeding Co., LTD., Hefei 340100, China .
authors.forename: Yueyun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Anhui Antai Pig Breeding Co., LTD., Hefei 340100, China .
authors.forename: Weiwei
authors.initials: W
authors.lastname: Xue
authors.affiliation: Anhui Antai Pig Breeding Co., LTD., Hefei 340100, China .
authors.forename: Yun
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Anhui Antai Pig Breeding Co., LTD., Hefei 340100, China .
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Anhui Antai Pig Breeding Co., LTD., Hefei 340100, China .
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Yin
keywords: Association; Expression; Natural Resistance-associated Macrophage Protein 1 (NRAMP1); Pig; Polymorphism
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