title: Point mutation of H3/H4 histones affects acetic acid tolerance in Saccharomyces cerevisiae.
abstract: The molecular mechanism of acetic acid tolerance in yeast remains unclear despite of its importance for efficient cellulosic ethanol production. In this study, we examined the effects of histone H3/H4 point mutations on yeast acetic acid tolerance by comprehensively screening a histone H3/H4 mutant library. A total of 24 histone H3/H4 mutants (six acetic acid resistant and 18 sensitive) were identified. Compared to the wild-type strain, the histone acetic acid-resistant mutants exhibited improved ethanol fermentation performance under acetic acid stress. Genome-wide transcriptome analysis revealed that changes in the gene expression in the acetic acid-resistant mutants H3 K37A and H4 K16Q were mainly related to energy production, antioxidative stress. Our results provide novel insights into yeast acetic acid tolerance on the basis of histone, and suggest a novel approach to improve ethanol production by altering the histone H3/H4 sequences.
authors.affiliation: Department of Cell Biology, Binzhou Medical University, Yantai 264003, China. Electronic address: liuxiangyong81@gmail.com.
authors.forename: Xiangyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Cell Biology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.
authors.forename: Zhaojie
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D019342:Acetic Acid / Q000633:toxicity*; D000595:Amino Acid Sequence; D001709:Biotechnology; D000431:Ethanol / Q000032:analysis / Q000378:metabolism; D005285:Fermentation / Q000235:genetics; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000235:genetics* / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000502:physiology
chemical_list: D006657:Histones; D000431:Ethanol; D019342:Acetic Acid
keywords: Acetic acid; Histone H3/H4; Point mutation; Saccharomyces cerevisiae