title: The cellular localization of Na(+)/H(+) exchanger 1, cystic fibrosis transmembrane conductance regulator, potassium channel, epithelial sodium channel γ and vacuolar-type H+-ATPase in human eccrine sweat glands.
abstract: The secretory portions of human eccrine sweat glands secrete isotonic fluid into the lumen and then the primary fluid is rendered hypotonic during its passage to the skin surface. During the processes of sweat secretion and absorption, many enzymes and proteins play important roles. In the study, the cellular localizations of Na(+)/H(+) exchanger 1 (NHE1), cystic fibrosis transmembrane conductance regulator (CFTR), potassium channel (KC), epithelial sodium channel γ (γENaC) and vacuolar-type H+-ATPase (V-ATPase) in human eccrine sweat glands and epidermis were detected using immunofluorescence labeling. The results revealed that in the secretory coils, the basolateral membranes showed evidence of CFTR, NHE1 and KC activities, the apical membranes showed the activities of KC and NHE1, and the nucleus showed γEaNC and V-ATPase activities; in the duct, the peripheral and luminal ductal cells showed evidence of CFTR, NHE1 and KC, the apical membranes showed the activities of CFTR and NHE1, and the nucleus showed γEaNC, V-ATPase and KC activities. The cellular localization of these proteins in eccrine sweat glands is helpful to better understand the mechanisms of sweat secretion and absorption.
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China; Research Center for Translational Medicine, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China. Electronic address: lihaihong1051@126.com.
authors.forename: Haihong
authors.initials: H
authors.lastname: Li
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Xuexue
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, 22 Xin Ling Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Changmin
authors.initials: C
authors.lastname: Lin
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Shenyou
authors.initials: S
authors.lastname: Shu
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Sitian
authors.initials: S
authors.lastname: Xie
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Yunpu
authors.initials: Y
authors.lastname: He
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China; Research Center for Translational Medicine, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Lvjun
authors.initials: L
authors.lastname: Yang
authors.affiliation: Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Tang
authors.affiliation: Burns Institute, The First Affiliated Hospital, Chinese PLA General Hospital, Trauma Center of Postgraduate Medical School, 51 Fu Cheng Road, Beijing 100037, PR China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
mesh_terms: D000328:Adult; D027682:Cation Transport Proteins / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism*; D004439:Eccrine Glands / Q000378:metabolism*; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D015221:Potassium Channels / Q000378:metabolism*; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism*
chemical_list: D027682:Cation Transport Proteins; D053503:Epithelial Sodium Channels; D015221:Potassium Channels; C490998:SLC9A1 protein, human; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D025262:Vacuolar Proton-Translocating ATPases
keywords: Cystic fibrosis transmembrane conductance regulator; Eccrine sweat gland; Epithelial sodium channel γ; Na(+)/H(+) exchanger 1; Potassium channel; Vacuolar-type H+-ATPase