title: A sensitive and effective proteomic approach to identify she-donkey's and goat's milk adulterations by MALDI-TOF MS fingerprinting.
abstract: She-donkey's milk (DM) and goat's milk (GM) are commonly used in newborn and infant feeding because they are less allergenic than other milk types. It is, therefore, mandatory to avoid adulteration and contamination by other milk allergens, developing fast and efficient analytical methods to assess the authenticity of these precious nutrients. In this experimental work, a sensitive and robust matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling was designed to assess the genuineness of DM and GM milks. This workflow allows the identification of DM and GM adulteration at levels of 0.5%, thus, representing a sensitive tool for milk adulteration analysis, if compared with other laborious and time-consuming analytical procedures.
authors.affiliation: Department of Laboratory Medicine, Bambino Gesù Children's Hospital, IRCCS,Piazza Sant'Onofrio 4, Rome 00165, Italy. francesco.digirolamo@opbg.net.
authors.forename: Francesco
authors.initials: F
authors.lastname: Di Girolamo
authors.affiliation: Gene Expression-Microarrays Laboratory, Bambino Gesù Children's Hospital, IRCCS,Piazza Sant'Onofrio 4, Rome 00165, Italy. andrea.masotti@opbg.net.
authors.forename: Andrea
authors.initials: A
authors.lastname: Masotti
authors.affiliation: Neonatal Intensive Care Unit, Department of Medical and Surgical Neonatology,Bambino Gesù Children's Hospital, IRCCS, Piazza Sant'Onofrio 4, Rome 00165, Italy. guglielmo.salvatori@opbg.net.
authors.forename: Guglielmo
authors.initials: G
authors.lastname: Salvatori
authors.affiliation: Department of Laboratory Medicine, San Camillo Hospital, Viale Vittorio Veneto 18,Treviso 31100, Italy. scapaticci.m@gmail.com.
authors.forename: Margherita
authors.initials: M
authors.lastname: Scapaticci
authors.affiliation: Department of Laboratory Medicine, Bambino Gesù Children's Hospital, IRCCS,Piazza Sant'Onofrio 4, Rome 00165, Italy. maurizio.muraca@opbg.net.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Muraca
authors.affiliation: Parasitology Unit, Bambino Gesù Children's Hospital, IRCCS, Piazza Sant'Onofrio 4,Rome 00165, Italy. lorenza.putignani@opbg.net.
authors.forename: Lorenza
authors.initials: L
authors.lastname: Putignani
mesh_terms: D000485:Allergens / Q000032:analysis; D000818:Animals; D002417:Cattle; D016000:Cluster Analysis; D018397:Equidae; D006041:Goats; D008892:Milk / Q000737:chemistry*; D025341:Principal Component Analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000485:Allergens
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