title: Observation of glycolytic metabolites in tumor cell lysate by using hyperpolarization of deuterated glucose.
abstract: Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of (13)C6-glucose-d7 and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin-lattice relaxation time T1. (13)C6-Glucose-d7 exhibited a T1 that was over ten times as long as that of (13)C6-glucose, and metabolic NMR studies of hyperpolarized (13)C6-glucose-d7 using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.
authors.affiliation: Science Research Center, Kochi University.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kumagai
authors.forename: Mai
authors.initials: M
authors.lastname: Akakabe
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tsuda
authors.forename: Masashi
authors.initials: M
authors.lastname: Tsuda
authors.forename: Eri
authors.initials: E
authors.lastname: Fukushi
authors.forename: Jun
authors.initials: J
authors.lastname: Kawabata
authors.forename: Takamasa
authors.initials: T
authors.lastname: Abe
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ichikawa
mesh_terms: D002247:Carbon Isotopes; D045744:Cell Line, Tumor; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy
chemical_list: D002247:Carbon Isotopes; D005947:Glucose