title: Assembly, characterization, and delivery of quantum dot labeled biotinylated lipid particles.
abstract: Lipid nanoparticles composed of mixtures of PEGylated-lipids; cationic and neutral lipids prepared by detergent dialysis can encapsulate biological active molecules and show considerable potential as systemic therapeutic agents. Addition of biotinylated lipids to this formulation allows surface modification of these particles with a suitable ligand or probe conjugated to streptavidin for specific cell targeting. Monitoring long circulating particles and cellular uptake requires stable and bright fluorescent probes. Quantum dots (QDs) constitute a relatively new class of fluorescent probes that overcome the limitations of organic fluorophores in biological imaging applications. Here, a protocol for the encapsulation of QD655 (red) in biotinylated lipid particles (BLPs) prepared by a detergent dialysis technique is presented followed by characterization of the loaded liposomal vehicles. Then, a protocol for BLPs surface modification via biotin-streptavidin linkage with preformed complexes of ligand-QD525 (green) for specific cell targeting of the nanoparticle is detailed. Conditions for cell binding and uptake of two colors QD labeled BLPs as well as basic microscopic settings for confocal live cell imaging are described.
authors.affiliation: Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry, 37077, Götingen, Germany, vsigot@bioingenieria.edu.ar.
authors.forename: Valeria
authors.initials: V
authors.lastname: Sigot
mesh_terms: D001710:Biotin / Q000378:metabolism; D019921:Biotinylation; D045744:Cell Line, Tumor; D003116:Color; D004337:Drug Carriers / Q000737:chemistry*; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D010316:Particle Size; D045663:Quantum Dots / Q000737:chemistry*; D013194:Staining and Labeling; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D013395:Sucrose / Q000737:chemistry; D013499:Surface Properties; D014461:Ultracentrifugation
chemical_list: D004337:Drug Carriers; D008055:Lipids; D013395:Sucrose; D004815:Epidermal Growth Factor; D001710:Biotin; D019809:Streptavidin