title: Prevalence of tonsillar human papillomavirus infections in Denmark.
abstract: The incidence of tonsillar carcinomas associated with Human Papillomavirus (HPV) infection has increased dramatically over the last three decades. In fact, currently in Scandinavia, HPV-associated cases account for over 80 % of tonsillar carcinoma cases. Yet, the epidemiology and natural history of tonsillar HPV infections remains poorly characterized. Our aim was to characterize such infections in the Danish population in tumor-free tonsillar tissue. Unlike previous studies, we considered both palatine tonsils. We examined both tonsils from 80 patients with peritonsillar abscess (n = 25) or chronic tonsillar disease (n = 55). HPV was detected by nested PCR with PGMY 09/11 and GP5+/GP6+L1 consensus primers, and typed by sequencing. Samples were also analyzed using a higher-throughput method, the CLART HPV 2 Clinical Array Assay. The overall prevalence of HPV tonsillar infection was 1.25 % (1/80, 95 % CI 0.03-6.77 %) by nested PCR, and 0 % by CLART HPV2 Clinical Array. The HPV-positive patient was a 16-year-old female with recurrent tonsillitis and tonsillar hypertrophy. The type detected was HPV6. HPV was not detected in the contralateral tonsil of this patient. Compared to cervical HPV infections in Denmark, tonsillar HPV infections are 10- to 15-fold less frequent. In the HPV-positive patient in this study, HPV was detected in only one of the tonsils. This raises the possibility that prior studies may underestimate the prevalence of HPV infections, as they do not consider both palatine tonsils.
authors.affiliation: Department of Otorhinolaryngology, Aarhus University Hospital, Aarhus, Denmark, maria.rusan@ki.au.dk.
authors.forename: M
authors.initials: M
authors.lastname: Rusan
authors.forename: T E
authors.initials: TE
authors.lastname: Klug
authors.forename: J J
authors.initials: JJ
authors.lastname: Henriksen
authors.forename: J H
authors.initials: JH
authors.lastname: Bonde
authors.forename: K
authors.initials: K
authors.lastname: Fuursted
authors.forename: T
authors.initials: T
authors.lastname: Ovesen
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002908:Chronic Disease; D003718:Denmark / Q000453:epidemiology; D005260:Female; D052163:Human papillomavirus 6 / Q000302:isolation & purification; D006801:Humans; D008297:Male; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D015995:Prevalence; D014069:Tonsillitis / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D055815:Young Adult
references.citation: Laryngoscope. 2006 Aug;116(8):1372-4
references.pmid: 16885738
references.citation: Clin Cancer Res. 2002 Apr;8(4):1093-100
references.pmid: 11948119
references.citation: Ann Otol Rhinol Laryngol. 2009 Oct;118(10):742-9
references.pmid: 19894403
references.citation: Cancer. 2001 Dec 1;92(11):2875-84
references.pmid: 11753961
references.citation: Sex Transm Dis. 2012 Jul;39(7):559-66
references.pmid: 22706220
references.citation: Arch Otolaryngol Head Neck Surg. 2009 Jun;135(6):554-7
references.pmid: 19528402
references.citation: J Virol. 2014 Jan;88(1):710-6
references.pmid: 24173230
references.citation: Sex Transm Infect. 2012 Dec;88(8):627-32
references.pmid: 22773328
references.citation: Oral Oncol. 2008 Feb;44(2):174-9
references.pmid: 17418614
references.citation: Vaccine. 2012 Nov 20;30 Suppl 5:F12-23
references.pmid: 23199955
references.citation: J Clin Microbiol. 2012 Jul;50(7):2289-98
references.pmid: 22535980
references.citation: Int J Cancer. 2011 Aug 1;129(3):733-41
references.pmid: 20878955
references.citation: J Clin Pathol. 2004 May;57(5):449-55
references.pmid: 15113849
references.citation: Cytokine. 2013 Nov;64(2):597-604
references.pmid: 24021705
references.citation: Eur J Clin Microbiol Infect Dis. 2012 Sep;31(9):2335-43
references.pmid: 22373896
references.citation: Sex Transm Dis. 2008 Mar;35(3):276-82
references.pmid: 18091564
references.citation: Pediatr Infect Dis J. 2006 Dec;25(12):1158-62
references.pmid: 17133162
references.citation: Lancet. 2013 Sep 7;382(9895):877-87
references.pmid: 23827089
references.citation: Int J Pediatr Otorhinolaryngol. 2010 Jan;74(1):7-14
references.pmid: 19800138
references.citation: J Natl Cancer Inst. 2013 Apr 17;105(8):536-45
references.pmid: 23503618
references.citation: Int J Cancer. 2014 Jun 15;134(12):2755-63
references.pmid: 23913554
references.citation: Mod Pathol. 2005 Apr;18(4):519-27
references.pmid: 15696119
references.citation: BMC Cancer. 2009 Dec 15;9:440
references.pmid: 20003490
references.citation: Gynecol Oncol. 2010 May;117(2 Suppl):S5-10
references.pmid: 20304221
references.citation: J Virol Methods. 2004 Dec 1;122(1):87-93
references.pmid: 15488625
references.citation: J Clin Microbiol. 2000 Jan;38(1):357-61
references.pmid: 10618116
references.citation: Eur J Pediatr. 2011 Jun;170(6):757-61
references.pmid: 21107606
references.citation: J Pediatr. 2012 May;160(5):837-43
references.pmid: 22137368
references.citation: J Gen Virol. 1995 Apr;76 ( Pt 4):1057-62
references.pmid: 9049358
references.citation: J Clin Pathol. 1990 Nov;43(11):918-21
references.pmid: 2175756
references.citation: Curr Opin Virol. 2013 Aug;3(4):416-21
references.pmid: 23816390
references.citation: J Clin Microbiol. 2005 Mar;43(3):1408-10
references.pmid: 15750119
references.citation: PLoS One. 2013;8(1):e53413
references.pmid: 23301068
references.citation: PLoS One. 2013 Jul 17;8(7):e68329
references.pmid: 23873171
references.citation: Int J Cancer. 2000 May 20;89(3):300-4
references.pmid: 10861508