title: Relationship between CYP7A1 -204A>C polymorphism with gallbladder stone disease and serum lipid levels: a meta-analysis.
abstract: BACKGROUND
  The CYP7A1 gene polymorphism has been reported to be associated with gallbladder stone disease (GSD) and serum lipid levels, but the results were inconsistent. This meta-analysis aimed to evaluate the influence of the -204A>C polymorphism in the promoter of CYP7A1 gene on the GSD and serum lipid levels.
  
  
  METHODS
  According to inclusion/exclusion criteria, eligible studies on CYP7A1 gene -204A>C polymorphism of serum lipid levels and the risk of GSD were retrieved. Depending on the between-study heterogeneity, the fixed- or random-effects model was applied, and the data were analyzed using the RevMan software (V5.2).
  
  
  RESULTS
  Five studies totaling 830 GSD patients and 882 healthy controls were used to evaluate the relation of CYP7A1 -204A>C polymorphism with GSD. Overall comparison of alleles A with C in all study population yielded 5% but non-significant increased risk of GSD (OR=1.05, 95% CI: 0.91 - 1.22, P=0.48). Subgroup analysis by ethnic differences did not show any association between CYP7A1 -204A>C polymorphism and GSD either. Four studies totaling 802 cases and 691 controls were used to assess the relation of CYP7A1 -204A>C polymorphism with serum lipid levels. All the subjects were from the Asian population. The pooled effects indicated that AC genotype had higher levels of TG than AA (MD=-0.42, 95% CI: -0.76 - -0.08, P=0.01). CC genotype in cases had higher levels of TC (MD=0.65, 95% CI: 0.25 - 1.05, P=0.001) and LDL-C (MD=0.40, 95% CI: 0.06 - 0.73, P=0.02) than AA, AA (MD = -0.35, 95% CI: -0.60 - -0.10, P=0.007) and AC (MD=-0.35, 95% CI: -0.61 - -0.08, P=0.01) genotypes in controls had higher levels of TC than CC, and AA genotype in controls had higher levels of HDL-C than CC (MD = -0.15, 95% CI: -0.21 - -0.09, P<0.00001).
  
  
  CONCLUSIONS
  The CYP7A1 -204A>C polymorphism is significantly associated with serum lipid levels in Asian population, but not gallbladder stone disease.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cai
authors.forename: Zhen-Qiang
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Qu
authors.initials: Q
authors.lastname: Cai
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Er-Zhen
authors.initials: EZ
authors.lastname: Chen
authors.affiliation: Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai JiaoTong University School of Medicine, 200025 Shanghai, China. zhaoyanjiang@gmail.com.
authors.forename: Zhao-Yan
authors.initials: ZY
authors.lastname: Jiang
mesh_terms: D016022:Case-Control Studies; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics*; D042882:Gallstones / Q000097:blood / Q000235:genetics*; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008055:Lipids / Q000097:blood*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: D008055:Lipids; C553219:CYP7A1 protein, human; D002790:Cholesterol 7-alpha-Hydroxylase
references.citation: World J Gastroenterol. 2010 Oct 7;16(37):4685-90
references.pmid: 20872969
references.citation: Metabolism. 2010 Jun;59(6):767-73
references.pmid: 20005541
references.citation: Nat Clin Pract Gastroenterol Hepatol. 2005 Sep;2(9):423-33
references.pmid: 16265433
references.citation: Gut Liver. 2012 Apr;6(2):172-87
references.pmid: 22570746
references.citation: Yi Chuan. 2004 May;26(3):283-6
references.pmid: 15640003
references.citation: World J Gastroenterol. 2004 May 15;10(10):1508-12
references.pmid: 15133863
references.citation: Clin Biochem. 2003 Jun;36(4):263-7
references.pmid: 12810154
references.citation: BMJ. 1997 Sep 13;315(7109):629-34
references.pmid: 9310563
references.citation: Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Aug;24(4):432-6
references.pmid: 17680536
references.citation: Genet Test. 2008 Mar;12(1):97-100
references.pmid: 18307386
references.citation: J Nutr. 2004 Sep;134(9):2200-4
references.pmid: 15333704
references.citation: J Clin Invest. 1998 Mar 15;101(6):1283-91
references.pmid: 9502769
references.citation: DNA Cell Biol. 2009 Sep;28(9):451-60
references.pmid: 19558216
references.citation: J Natl Cancer Inst. 1959 Apr;22(4):719-48
references.pmid: 13655060
references.citation: Control Clin Trials. 1986 Sep;7(3):177-88
references.pmid: 3802833
references.citation: Ann Intern Med. 1997 Nov 1;127(9):820-6
references.pmid: 9382404
references.citation: Stat Med. 2002 Jun 15;21(11):1539-58
references.pmid: 12111919
references.citation: J Cardiovasc Pharmacol. 2012 Mar;59(3):254-9
references.pmid: 22075751
references.citation: Hepatology. 2005 May;41(5):1138-43
references.pmid: 15747383
references.citation: Clin Nutr. 2011 Apr;30(2):239-46
references.pmid: 20884100
references.citation: Atherosclerosis. 2001 Feb 15;154(3):579-87
references.pmid: 11257258
references.citation: J Lipid Res. 1999 Oct;40(10):1883-9
references.pmid: 10508208
references.citation: Curr Gastroenterol Rep. 2004 Apr;6(2):140-50
references.pmid: 15191694
references.citation: J Clin Invest. 2002 Jul;110(1):109-17
references.pmid: 12093894
references.citation: BMJ. 2003 Sep 6;327(7414):557-60
references.pmid: 12958120