title: Preparative isolation of paclitaxel and related taxanes from cell cultures of Taxus chinensis using reversed-phase flash chromatography.
abstract: In this study, paclitaxel, baccatin III, taxuyunnanine C and sinenxane C were successfully separated by reversed-phase flash chromatography on a manually packed C18 column from Taxus chinensis cell culture extract. The crude cell culture extract was first treated with Al2O3 column chromatography and then divided into two parts: fraction 1 and fraction 2. Ten milligrams of baccatin III and 19 mg of paclitaxel were obtained from 100 mg dried fraction 1. Fifty-two milligrams of taxuyunnanine C and 11 mg sinenxane C were obtained from 100 mg dried fraction 2. The purities of the four compounds were 98.02%, 98.53%, 98.93% and 98.76%, respectively. Their structures were characterised by using UV, MS and NMR. These results indicate that paclitaxel and related taxanes including baccatin III can be obtained from cell culture in a highly pure state using reversed-phase flash chromatography.
authors.affiliation: a School of Pharmaceutical Sciences, Sun Yat-Sen University , Guangzhou 510006 , P.R. China.
authors.forename: Zhi-Kun
authors.initials: ZK
authors.lastname: Liang
authors.forename: Ruo-Gu
authors.initials: RG
authors.lastname: Huang
authors.forename: Zhi-Sheng
authors.initials: ZS
authors.lastname: Xie
authors.forename: Xin-Jun
authors.initials: XJ
authors.lastname: Xu
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification; D018929:Cell Culture Techniques; D056148:Chromatography, Reverse-Phase; D017239:Paclitaxel / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D043823:Taxoids / Q000302:isolation & purification*; D020946:Taxus / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D043823:Taxoids; C494600:taxuyunnanine C; C073950:baccatin III; D017239:Paclitaxel
keywords: Taxus chinensis cell culture; baccatin III; paclitaxel; reversed-phase flash chromatography; sinenxane C; taxuyunnanine C