title: Spermidine biosynthesis and transport modulate pneumococcal autolysis.
abstract: Polyamines are small cationic molecules that have far-reaching roles in biology. In the case of pathogenic bacteria, these functions include those central to their pathogenesis. Streptococcus pneumoniae is a major bacterial pathogen, causing a diverse range of diseases that account for significant morbidity and mortality worldwide. In this work, we characterize the polyamine biosynthetic pathway of S. pneumoniae, demonstrating that this organism produces spermidine from arginine. The synthesis of spermidine was found to be nonessential for growth in a polyamine-free chemically defined medium. However, mutant strains lacking the ability to synthesize or transport spermidine displayed a significant delay in the onset of autolysis. We provide evidence for a model in which spermidine modulates the activity of the major autolysin LytA in the pneumococcal cell wall compartment via interactions with negatively charged molecules, such as teichoic acids.
authors.affiliation: Research Centre for Infectious Diseases, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Potter
authors.affiliation: Research Centre for Infectious Diseases, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia, Australia james.paton@adelaide.edu.au.
authors.forename: James C
authors.initials: JC
authors.lastname: Paton
mesh_terms: D001433:Bacteriolysis / Q000502:physiology*; D001692:Biological Transport / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D013095:Spermidine / Q000096:biosynthesis*; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000378:metabolism
chemical_list: D013095:Spermidine
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