title: Glycan structure and site of glycosylation in the ER-resident glycoprotein, uridine 5'-diphosphate-glucose: glycoprotein glucosyltransferases 1 from rat, porcine, bovine, and human.
abstract: Here we report glycan structures and their position of attachment to a carrier protein, uridine 5'-diphosphate-glucose: glycoprotein glucosyltransferase (UGGT1), as detected using tandem mass spectrometry. UGGT1 acts as a folding sensor of newly synthesized glycosylated polypeptides in the endoplasmic reticulum, and the transferase itself is known to be glycosylated. The structure of glycan attached to UGGT1, however, has not been investigated. In this study, we reveal the site of glycosylation (N269) and the glycan structures (Hex5-8HexNAc2) in UGGT1 obtained from rat (Rattus norvegicus), pig (Sus scrofa), cow (Bos taurus), and human (Homo sapiens).
authors.affiliation: Ito Glycotrilogy Project, ERATO, Japan Science and Technology Agency (JST), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Shusaku
authors.initials: S
authors.lastname: Daikoku
authors.affiliation: Ito Glycotrilogy Project, ERATO, Japan Science and Technology Agency (JST), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Seko
authors.affiliation: Ito Glycotrilogy Project, ERATO, Japan Science and Technology Agency (JST), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan; Synthetic Cellular Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Yukishige
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Ito Glycotrilogy Project, ERATO, Japan Science and Technology Agency (JST), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan; Institute of Glycoscience, Tokai University, 4-1-1 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan. Electronic address: kanie@tokai-u.jp.
authors.forename: Osamu
authors.initials: O
authors.lastname: Kanie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005964:Glucosyltransferases / Q000737:chemistry* / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D017510:Protein Folding; D051381:Rats; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D011134:Polysaccharides; D005964:Glucosyltransferases
keywords: Glycan structure; NanoLC–MS(n); Uridine 5′-diphosphate (UDP)-glucose: glycoprotein glucosyltransferase 1