title: Novel synthesis of gold nanoclusters templated with L-tyrosine for selective analyzing tyrosinase.
abstract: L-Tyrosine (Tyr), playing roles as both a reducing reagent and a protecting ligand, has been first employed for synthesizing fluorescent gold nanoclusters (AuNCs@Tyr) via a novel one-pot strategy. The as-prepared AuNCs@Tyr exhibited a fluorescence emission at 470 nm with a quantum yield of approximately 2.5%. Subsequently, the AuNCs@Tyr described here was applied for detections of tyrosinase (TR) activity, which was based on the mechanism of aggregations of AuNCs@Tyr occurring on the active sites of TR since TR was introduced, thus leading to the fluorescence quenching of AuNCs@Tyr. Accordingly, TR was analyzed in a linear range of 0.5-200 u mL(-1) with a detection limit of 0.08 u mL(-1) at a signal-to-noise ratio of 3. Significantly, TR has been considered as a critical marker for melanoma owing to its specifically expressing in melanoma cells. Therefore, this analytical method towards investigating TR activity may broaden avenues for meaningfully clinical applications.
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Tiansheng Road No. 2, Beibei District, Chongqing 400716, China. Electronic address: ming4444@swu.edu.cn.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Tiansheng Road No. 2, Beibei District, Chongqing 400716, China.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Luo
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Tiansheng Road No. 2, Beibei District, Chongqing 400716, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhuo
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Tiansheng Road No. 2, Beibei District, Chongqing 400716, China.
authors.forename: Yuanjiao
authors.initials: Y
authors.lastname: Feng
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Tiansheng Road No. 2, Beibei District, Chongqing 400716, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhu
mesh_terms: D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000032:analysis* / Q000737:chemistry; D017433:Protein Structure, Secondary; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D014443:Tyrosine; D006046:Gold; D014442:Monophenol Monooxygenase
keywords: Fluorescence quenching; Gold nanoclusters; Tyrosinase; Tyrosine