title: Characterization of seven new polymorphic microsatellite loci in the brilliant-thighed poison frogAllobates femoralis(Dendrobatidae), and their cross-species utility in three other dendrobatid species.
abstract: Here we document the development of seven novel polymorphic microsatellite markers for the brilliant-thighed poison frogAllobates femoralis(Dendrobatidae). We found between six and 27 alleles per locus in 100 individuals (50 males, 50 females) from the field site 'Saut Pararé', French Guiana, with an average observed heterozygosity of 0.79. One locus (Afem23) deviated significantly from Hardy-Weinberg equilibrium. We did not find any evidence for linkage disequilibrium among the new loci, or to seven of the already described markers forA. femoralis. We also report cross-species amplification of some of the markers in three other dendrobatid species (A. talamancae, Dendrobates tinctoriusandOophaga pumilio).
authors.affiliation: University of Vienna, Department of Integrative Zoology, Althanstraße 14, A-1090 Vienna, Austria ; University of Vienna, Department of Cognitive Biology, Althanstraße 14, A-1090 Vienna, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Ringler
authors.affiliation: University of Vienna, Department of Cognitive Biology, Althanstraße 14, A-1090 Vienna, Austria.
authors.forename: Andrius
authors.initials: A
authors.lastname: Pašukonis
authors.affiliation: University of Vienna, Department of Integrative Zoology, Althanstraße 14, A-1090 Vienna, Austria.
authors.forename: Walter
authors.initials: W
authors.lastname: Hödl
authors.affiliation: University of Vienna, Department of Integrative Zoology, Althanstraße 14, A-1090 Vienna, Austria ; University of Vienna, Department of Tropical Ecology and Animal Biodiversity, Rennweg 14, A-1030 Vienna, Austria.
authors.forename: Max
authors.initials: M
authors.lastname: Ringler
keywords: Allobates femoralis; Dendrobatidae; cross-species amplification; microsatellite marker; polymorphic
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