title: Nitric oxide-releasing porous silicon nanoparticles.
abstract: In this study, the ability of porous silicon nanoparticles (PSi NPs) to entrap and deliver nitric oxide (NO) as an effective antibacterial agent is tested against different Gram-positive and Gram-negative bacteria. NO was entrapped inside PSi NPs functionalized by means of the thermal hydrocarbonization (THC) process. Subsequent reduction of nitrite in the presence of d-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells. The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties. These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.
authors.forename: Morteza Hasanzadeh
authors.initials: MH
authors.lastname: Kafshgari
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.
authors.forename: Alex
authors.initials: A
authors.lastname: Cavallaro
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.
authors.forename: Bahman
authors.initials: B
authors.lastname: Delalat
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.
authors.forename: Frances J
authors.initials: FJ
authors.lastname: Harding
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.
authors.forename: Steven Jp
authors.initials: SJ
authors.lastname: McInnes
authors.affiliation: Department of Physics and Astronomy, University of Turku, Turku FI-20014, Finland.
authors.forename: Ermei
authors.initials: E
authors.lastname: Mäkilä
authors.affiliation: Department of Physics and Astronomy, University of Turku, Turku FI-20014, Finland.
authors.forename: Jarno
authors.initials: J
authors.lastname: Salonen
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.
authors.forename: Krasimir
authors.initials: K
authors.lastname: Vasilev
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia, GPO Box 2471 Adelaide, SA 5001, Australia.
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
keywords: Antibacterial; Nitric oxide; Porous silicon nanoparticles
references.citation: Adv Healthc Mater. 2013 Oct;2(10):1351-60
references.pmid: 23526816
references.citation: J Pharm Sci. 2008 Feb;97(2):632-53
references.pmid: 17546667
references.citation: Lancet. 2001 Jul 14;358(9276):135-8
references.pmid: 11463434
references.citation: Nitric Oxide. 2008 Aug;19(1):12-20
references.pmid: 18457680
references.citation: Anal Biochem. 2002 Aug 1;307(1):167-72
references.pmid: 12137794
references.citation: Metabolism. 2002 Oct;51(10):1269-73
references.pmid: 12370845
references.citation: Expert Opin Drug Deliv. 2009 Oct;6(10):1113-22
references.pmid: 19663720
references.citation: Clin Infect Dis. 2013 Jun;56(11):1614-20
references.pmid: 23362298
references.citation: J Am Acad Dermatol. 2008 Feb;58(2):185-206
references.pmid: 18222318
references.citation: Biomaterials. 2005 Mar;26(8):917-24
references.pmid: 15353203
references.citation: Dermatol Online J. 2013 Feb 15;19(2):7
references.pmid: 23473277
references.citation: Future Med Chem. 2009 Sep;1(6):1051-74
references.pmid: 21425994
references.citation: Dermatol Online J. 2013 Feb 15;19(2):5
references.pmid: 23473275
references.citation: Anal Chem. 1998 Jul 1;70(13):2446-53
references.pmid: 9666719
references.citation: ACS Nano. 2011 Sep 27;5(9):7235-44
references.pmid: 21842899
references.citation: J Biol Chem. 2006 Nov 24;281(47):36021-8
references.pmid: 17005567
references.citation: Am J Surg. 2002 Apr;183(4):406-12
references.pmid: 11975928
references.citation: Environ Sci Technol. 2007 Mar 15;41(6):2064-8
references.pmid: 17410806
references.citation: Arch Med Res. 2005 Sep-Oct;36(5):464-7
references.pmid: 16099322
references.citation: J Invest Dermatol. 2009 Oct;129(10):2463-9
references.pmid: 19387479
references.citation: Adv Drug Deliv Rev. 2008 Aug 17;60(11):1266-77
references.pmid: 18508154
references.citation: ACS Nano. 2009 Feb 24;3(2):279-90
references.pmid: 19236062
references.citation: Ann Biomed Eng. 2005 Feb;33(2):214-22
references.pmid: 15771275
references.citation: Acta Biomater. 2010 Jul;6(7):2721-31
references.pmid: 20036766
references.citation: Microbiol Mol Biol Rev. 1997 Dec;61(4):533-616
references.pmid: 9409151
references.citation: ACS Nano. 2008 Feb;2(2):235-46
references.pmid: 19206623
references.citation: Trends Biotechnol. 2012 Oct;30(10):499-511
references.pmid: 22884769
references.citation: ACS Nano. 2010 Jun 22;4(6):3023-32
references.pmid: 20509673
references.citation: Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10480-4
references.pmid: 1720542
references.citation: Biomaterials. 2009 May;30(14):2782-9
references.pmid: 19233464
references.citation: Nitric Oxide. 2006 Feb;14(1):21-9
references.pmid: 16188471
references.citation: Biomaterials. 2011 Apr;32(10):2625-33
references.pmid: 21194747
references.citation: Nanoscale Res Lett. 2012 Jun 19;7:311
references.pmid: 22713167
references.citation: Int Wound J. 2015 Apr;12(2):169-72
references.pmid: 23556996
references.citation: Carbohydr Res. 2004 Nov 15;339(16):2693-700
references.pmid: 15519328
references.citation: J Clin Microbiol. 1998 Jun;36(6):1683-7
references.pmid: 9620400
references.citation: World J Surg. 2004 Mar;28(3):301-6
references.pmid: 14961192
references.citation: Biotechnol Adv. 2009 Jan-Feb;27(1):76-83
references.pmid: 18854209