title: In vitro efficacy of doxorubicin and etoposide against a feline injection site sarcoma cell line.
abstract: Feline injection site sarcoma (ISS) is a locally invasive tumor, in which surgical treatment is frequently combined with radiation or chemotherapy to improve tumor control. The focus of this study was to evaluate the cytotoxic effects of doxorubicin or etoposide on a feline injection site sarcoma cell line (JB) and to assess the impact of combining these drugs on cell death and cell cycle. Both single agent and combination drug administration increased cell death and significantly reduced the number of viable cells. Cells in G0/G1 were significantly reduced while the G2/M fraction was significantly increased following treatment. Collectively, combining doxorubicin and etoposide at the lower EC yielded comparable results to the EC50 of either drug alone in degree of cytotoxicity, level of apoptosis, and % of cells in G2/M. The results of this study indicate that doxorubicin and etoposide alone and in combination differentially alter ISS cell viability and cycle.
authors.affiliation: Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Georgia, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Hill
authors.affiliation: Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Georgia, USA; Veterinary Cancer Centre, The Royal School of Veterinary Studies, University of Edinburgh, UK.
authors.forename: Jessica
authors.initials: J
authors.lastname: Lawrence
authors.affiliation: Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Georgia, USA.
authors.forename: Corey
authors.initials: C
authors.lastname: Saba
authors.affiliation: Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Georgia, USA; Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Georgia, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Turek
authors.affiliation: Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Georgia, USA.
authors.forename: Brittany
authors.initials: B
authors.lastname: Feldhaeusser
authors.affiliation: Department of Pathology, College of Veterinary Medicine, University of Georgia, Georgia, USA.
authors.forename: Sheryl
authors.initials: S
authors.lastname: Coutermarsh-Ott
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Georgia, USA.
authors.forename: Jamie
authors.initials: J
authors.lastname: Barber
authors.affiliation: Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Georgia, USA.
authors.forename: Jo
authors.initials: J
authors.lastname: Smith
authors.affiliation: Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Georgia, USA; Department of Pathology, College of Veterinary Medicine, University of Georgia, Georgia, USA. Electronic address: rgogal@uga.edu.
authors.forename: Robert
authors.initials: R
authors.lastname: Gogal
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D002371:Cat Diseases / Q000188:drug therapy / Q000473:pathology*; D002415:Cats; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology* / Q000627:therapeutic use; D004359:Drug Therapy, Combination; D005047:Etoposide / Q000494:pharmacology* / Q000627:therapeutic use; D066298:In Vitro Techniques; D009372:Neoplasms, Connective Tissue / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D012509:Sarcoma / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*
chemical_list: D000970:Antineoplastic Agents; D005047:Etoposide; D004317:Doxorubicin
keywords: Apoptosis; Cell cycle; Doxorubicin; Etoposide; Injection site sarcoma