title: Bioethanol production from wheat straw via enzymatic route employing Penicillium janthinellum cellulases.
abstract: This study concerns in-house development of cellulases from a mutant Penicillium janthinellum EMS-UV-8 and its application in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes for bioethanol production from pre-treated wheat straw. In a 5L fermentor, the above strain could produce cellulases having activity of 3.1 FPU/mL and a specific activity of 0.83 FPU/mg of protein. In-house developed cellulase worked more efficiently in case of SSF as ethanol concentration of 21.6g/L and yield of 54.4% were obtained which were higher in comparison to SHF (ethanol concentration 12 g/L and 30.2% yield). This enzyme preparation when compared with commercial cellulase for hydrolysis of pre-treated wheat straw was found competitive. This study demonstrates that P. janthinellum EMS-UV-8 is a potential fungus for future large-scale production of cellulases.
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Reeta Rani
authors.initials: RR
authors.lastname: Singhania
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Jitendra Kumar
authors.initials: JK
authors.lastname: Saini
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Reetu
authors.initials: R
authors.lastname: Saini
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Mukund
authors.initials: M
authors.lastname: Adsul
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Anshu
authors.initials: A
authors.lastname: Mathur
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: DBT-IOC Centre for Advanced Bio-Energy Research, Indian Oil Corporation Ltd., R&D Centre, Sector-13, Faridabad 121007, India. Electronic address: tulidk@indianoil.in.
authors.forename: Deepak Kumar
authors.initials: DK
authors.lastname: Tuli
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D044602:Cellulases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000378:metabolism*; D006868:Hydrolysis; D010407:Penicillium / Q000201:enzymology*; D014908:Triticum / Q000737:chemistry*; D014866:Waste Products
chemical_list: D056804:Biofuels; D014866:Waste Products; D000431:Ethanol; D044602:Cellulases
keywords: Bioethanol; Cellulase; Penicillium; SHF; SSF