title: Single-laboratory validation for the determination of caffeic acid and seven caffeoylquinic acids in sweet potato leaves.
abstract: A single-laboratory validation study was conducted on an HPLC method for the detection and quantification of caffeic acid (CA) and seven species of caffeoylquinic acids (CQAs) in lyophilized sweet potato leaves. The procedure for extraction of the analytes from the matrix and the HPLC conditions for the efficient separation of CA and CQAs were optimized. In the proposed method, a relative response factor to one of the CQAs (5-CQA) was used to quantify the others. The method performed well in terms of precision when carried out on five different days and demonstrated Horwitz ratio (HorRat) scores ranging from 0.5 to 1.0 for all analytes, which were well within the limits of performance acceptability. Accuracy testing at three levels showed an overall recovery of 94% when duplicated on five different days. Moreover, a stability study revealed that all analytes in both standard solution and sample extract were stable for 28 days.
authors.affiliation: a Kyushu Okinawa Agricultural Research Center , National Agriculture and Food Research Organization , Koshi , Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Sasaki
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Oki
authors.forename: Toru
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kai
authors.forename: Shigenori
authors.initials: S
authors.lastname: Okuno
mesh_terms: D002109:Caffeic Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D005419:Flavonoids / Q000302:isolation & purification*; D005612:Freeze Drying; D027723:Ipomoea batatas / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002109:Caffeic Acids; D005419:Flavonoids; D010936:Plant Extracts; C472707:caffeoylquinic acid; D011801:Quinic Acid; C040048:caffeic acid
keywords: HPLC; caffeoylquinic acid; single-laboratory validation; sweet potato leaves