title: An in silico model for rapid identification of multiple bacteria in resource limited laboratories.
abstract: We have developed an in silico model for simplified detection of five major food borne bacteria using common PCR primer based on conserved region flanking a variable region in the first rRNA operon sequences. The RFLP pattern exhibited on virtual SmaI and MboI digestion were unique to each genus. This design is based on the combination of comparative sequence analysis, conventional PCR and restriction digestion methods. We believe this approach as a better alternative to 16S rRNA sequencing based identification / detection of bacteria and suited for the resource limited academic research laboratories in which variety of bacteria are used in different students' projects.
authors.affiliation: School of Bio Sciences and Technology, VIT University, Vellore, 632014, India, babu.s@vit.ac.in.
authors.forename: Subramanian
authors.initials: S
authors.lastname: Babu
authors.forename: Asit Ranjan
authors.initials: AR
authors.lastname: Ghosh
authors.forename: Ramamoorthy
authors.initials: R
authors.lastname: Siva
authors.forename: Chinnadurai Immanuel
authors.initials: CI
authors.lastname: Selvaraj
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001419:Bacteria / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D003198:Computer Simulation; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D005517:Foodborne Diseases / Q000382:microbiology*; D006295:Health Resources; D006801:Humans; D007753:Laboratories; D008954:Models, Biological; D008958:Models, Molecular; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D012407:rRNA Operon
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S