title: Extraction of antibiotic zwittermicin A from Bacillus thuringiensis by macroporous resin and silica gel column chromatography.
abstract: To establish a production process capable of providing refined zwittermicin A (ZwA) on a large scale, the macroporous resin and silica gel column chromatography were used to separate and purify the antibiotic ZwA from the fermentation broth of Bacillus thuringiensis HD-1. The result of high-performance liquid chromatography-mass spectrometry after purification suggests that the samples of ZwA were of high purity, 89%, and the average yield was 20 mg L(-1). Erwinia herbicola LS005, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis were used to assess the toxicity of ZwA. The antibiotic had strong antibacterial activity against E. herbicola LS005 and a color reaction with ninhydrin.
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, People's Republic of China.
authors.forename: Zaibin
authors.initials: Z
authors.lastname: Hao
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, People's Republic of China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
authors.forename: Fuping
authors.initials: F
authors.lastname: Song
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001413:Bacillus thuringiensis / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D002457:Cell Extracts / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D058428:Silica Gel / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D002457:Cell Extracts; D010455:Peptides; C088290:zwittermicin A; D058428:Silica Gel
keywords: Bacillus thuringiensis; antibiotics; purification; zwittermicin A