title: Flavobacterium oryzae sp. nov., isolated from a flooded rice field, and emended descriptions of Flavobacterium flevense, Flavobacterium yonginense and Flavobacterium myungsuense.
abstract: A bacterial strain, designated Jyi-05(T), was isolated from a flooded rice field and characterized using a polyphasic taxonomic approach. Cells of strain Jyi-05(T) were Gram-stain-negative, strictly aerobic, bright-yellow-pigmented rods that were motile by gliding. Growth occurred at 10-30 °C (optimum 30 °C), at pH 6.5-7.5 (optimum pH 7.0) and with 0-2% (w/v) NaCl (optimum 1%, w/v, NaCl). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Jyi-05(T) belonged to the genus Flavobacterium and was most closely related to Flavobacterium flevense DSM 1076(T) with sequence similarity of 97.6%. Strain Jyi-05(T) contained iso-C(15:0) (16.6%), anteiso-C(15:0) (10.8%), summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c; 10.3%), iso-C((17:0) 3-OH (9.2%), C(16:0) (7.3%) and iso-C(15:0) 3-OH (7.2%) as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. The genomic DNA G+C content of strain Jyi-05(T) was 34.2 mol%. The DNA-DNA relatedness of strain Jyi-05(T) with respect to recognized species of the genus Flavobacterium was less than 70%. On the basis of the phylogenetic inference and phenotypic data, strain Jyi-05(T) should be classified as a novel species, for which the name Flavobacterium oryzae sp. nov. is proposed. The type strain is Jyi-05(T) ( = BCRC 80522(T) =LMG 27292(T) =KCTC 32240(T)). Emended descriptions of Flavobacterium flevense, Flavobacterium yonginense and Flavobacterium myungsuense are also presented.
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142 Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142 Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Jhen-Ci
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142 Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000737:chemistry; D013624:Taiwan; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6; D013095:Spermidine