title: Atoh7 promotes retinal Müller cell differentiation into retinal ganglion cells.
abstract: Glaucoma is one of the leading eye diseases due to the death of retinal ganglion cells. Increasing evidence suggests that retinal Müller cells exhibit the characteristics of retinal progenitor cells and can differentiate to neurons in injured retinas under certain conditions. However, the number of ganglion cells differentiated from retinal Müller cells falls far short of therapeutic needs. This study aimed to promote the differentiation of retinal Müller cells into ganglion cells by introducing Atoh7 into the stem cells dedifferentiated from retinal Müller cells. Rat retinal Müller cells were isolated and dedifferentiated into stem cells, which were transfected with PEGFP-N1 or PEGFP-N1-Atoh7 vector, and then further induced to differentiate into ganglion cells. The proportion of ganglion cells differentiated from Atoh7-tranfected stem cells was significantly higher than that of control transfected or untransfected cells. In summary, Atoh7 promotes the differentiation of retinal Müller cells into retinal ganglion cells. This may open a new avenue for gene therapy of glaucoma by promoting optic nerve regeneration.
authors.affiliation: Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Wei-Tao
authors.initials: WT
authors.lastname: Song
authors.affiliation: Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, 410008, China. xbxia21@163.com.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Xia
authors.affiliation: State Key Lab of Medical Genetics of China, Central South University, Changsha, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xia
authors.affiliation: State Key Lab of Medical Genetics of China, Central South University, Changsha, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Pan
keywords: Atoh7; Müller cells; Progenitor cells; Retinal ganglion cells
references.citation: Curr Opin Neurobiol. 1999 Feb;9(1):37-46
references.pmid: 10072376
references.citation: J Neurosci. 1995 Aug;15(8):5765-78
references.pmid: 7643217
references.citation: Stem Cells Dev. 2008 Aug;17(4):837-48
references.pmid: 18752428
references.citation: Mol Vis. 2007 Jun 30;13:1066-72
references.pmid: 17653051
references.citation: Biotechniques. 1994 Dec;17(6):1058-62
references.pmid: 7873174
references.citation: Genes Dev. 2001 Jan 1;15(1):24-9
references.pmid: 11156601
references.citation: Dev Biol. 2003 Dec 1;264(1):240-54
references.pmid: 14623245
references.citation: J Neurosci Methods. 2002 Jun 30;117(2):153-8
references.pmid: 12100980
references.citation: J Neurosci. 2007 Feb 14;27(7):1712-24
references.pmid: 17301179
references.citation: Stem Cells. 2006 Dec;24(12):2776-91
references.pmid: 16902196
references.citation: Cell Tissue Res. 2001 Aug;305(2):171-6
references.pmid: 11545254
references.citation: Dev Biol. 2006 Nov 1;299(1):283-302
references.pmid: 16949068
references.citation: Science. 2000 Mar 17;287(5460):2032-6
references.pmid: 10720333
references.citation: Mol Cell Neurosci. 2001 Mar;17(3):426-43
references.pmid: 11273640
references.citation: Biochem Biophys Res Commun. 2006 Dec 15;351(2):514-20
references.pmid: 17070773
references.citation: Development. 2001 Jul;128(13):2497-508
references.pmid: 11493566
references.citation: Mol Vis. 2012;18:2658-72
references.pmid: 23170059
references.citation: Prog Retin Eye Res. 2006 Jul;25(4):397-424
references.pmid: 16839797
references.citation: Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13654-9
references.pmid: 15353594
references.citation: Stem Cells. 2007 Aug;25(8):2033-43
references.pmid: 17525239
references.citation: Development. 1998 Dec;125(23):4821-33
references.pmid: 9806930
references.citation: Invest Ophthalmol Vis Sci. 1998 Jan;39(1):212-6
references.pmid: 9430566
references.citation: BMC Dev Biol. 2007 May 15;7:45
references.pmid: 17504531
references.citation: J Neurosci Res. 2009 Mar;87(4):964-77
references.pmid: 18855938
references.citation: Biophys J. 1998 Jun;74(6):3015-22
references.pmid: 9635756