title: Long-term stabilization of enzyme activity by immobilizing enzyme colony in polymaleimidostyrene-modified micelles.
abstract: Amperometric biosensors fabricated by immobilizing enzyme colony in polymaleimidostyrene-modified micelles exhibited good sensitivity and great long-term stability. To our knowledge, there has not been a general design for various enzyme biosensors. Enzyme micelle membrane which is different from any of other conventional immobilization methods, is an innovative way and will be a well-developed biosensor technology to provide rapid and reliable measurements. The potential of using enzyme micelle membrane to fabricate biosensors will be of great hope.
authors.affiliation: Department of Materials and Science, Graduate School of Engineering, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293. Japan; School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Materials and Science, Graduate School of Engineering, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293. Japan.
authors.forename: Shunichi
authors.initials: S
authors.lastname: Uchiyama
mesh_terms: D002021:Buffers; D004563:Electrochemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008301:Maleimides / Q000737:chemistry*; D008823:Micelles; D008855:Microscopy, Electron, Scanning; D011137:Polystyrenes / Q000737:chemistry*; D012996:Solutions; D014508:Urea / Q000032:analysis; D014510:Urease / Q000378:metabolism*
chemical_list: D002021:Buffers; D004800:Enzymes, Immobilized; D008301:Maleimides; D008823:Micelles; D011137:Polystyrenes; D012996:Solutions; C512149:polymaleimidostyrene; D014508:Urea; D014510:Urease