title: Developmental programming of aging of isolated pancreatic islet glucose-stimulated insulin secretion in female offspring of mothers fed low-protein diets in pregnancy and/or lactation.
abstract: Diabetes predisposition is determined by pancreatic islet insulin secretion and insulin resistance. We studied female rat offspring exposed to low-protein maternal diet (50% control protein diet) in pregnancy and/or lactation at postnatal days 36, 110 and 450. Rats were fed either control 20% casein diet (C) or restricted diet (R - 10% casein) during pregnancy. After delivery, mothers received either C or R diet until weaning to provide four offspring groups: CC, RR, CR and RC (first letter denoting maternal pregnancy diet and the second lactation diet). Serum glucose, insulin and homeostatic model assessment (HOMA) were measured. Pancreatic islets were isolated and in vitro insulin secretion quantified in low glucose (5 mM) and high glucose (11 mM). Serum glucose, insulin and HOMA were similar in all groups at 36 and 110 postnatal days. HOMA was only higher in RR at 450 postnatal days. Only CC demonstrated differences in glucose sensitivity of β-cells to high and low doses at the three ages studied. At 36 days, RR, CR and RC and at 450 days RR and RC groups did not show glucose-stimulated insulin secretion differences between low and high glucose. Aging-associated glucose-stimulated insulin secretion loss was affected by maternal dietary history, indicating that developmental programming must be considered a major factor in aging-related development of predisposition to later-life dysfunctional insulin metabolism. Female offspring islets' insulin secretion was higher than previously reported in males.
authors.affiliation: 1 Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City, Mexico.
authors.forename: S
authors.initials: S
authors.lastname: Morimoto
authors.affiliation: 1 Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City, Mexico.
authors.forename: T C
authors.initials: TC
authors.lastname: Sosa
authors.affiliation: 1 Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City, Mexico.
authors.forename: L
authors.initials: L
authors.lastname: Calzada
authors.affiliation: 1 Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City, Mexico.
authors.forename: L A
authors.initials: LA
authors.lastname: Reyes-Castro
authors.affiliation: 1 Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Díaz-Díaz
authors.affiliation: 1 Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Morales
authors.affiliation: 2 Departament of Gynecology, Center for Pregnancy and Newborn Research, UTHSCSA, San Antonio Texas, USA.
authors.forename: P W
authors.initials: PW
authors.lastname: Nathanielsz
authors.affiliation: 1 Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México City, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Zambrano
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D018753:Diet, Protein-Restricted; D005260:Female; D047109:Fetal Development; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D007774:Lactation / Q000378:metabolism*; D008297:Male; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics
chemical_list: D001786:Blood Glucose; D007328:Insulin; D005947:Glucose