title: Use of multiplex PCR in diagnosis of bloodstream infections in kidney patients.
abstract: The LightCycler® SeptiFast Test (Roche Diagnostics GmbH, Mannheim, Germany) was prospectively compared with the standard blood culture technique in a series of 86 kidney patients. The sensitivity of the PCR compared with the culture was 71%, and the specificity was 88%. All the species identified by culture in these patients were in the SeptiFast panel. The median time to results was 1 day for the PCR, 3 days for positive cultures, and 5 days for negative cultures.
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046, Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Fernández-Romero
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046, Madrid, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Quiles
authors.affiliation: Servicio de Nefrología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046, Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Jiménez
authors.affiliation: Servicio de Nefrología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046, Madrid, Spain.
authors.forename: María Ovidea Lopez
authors.initials: MO
authors.lastname: Oliva
authors.affiliation: Servicio de Nefrología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046, Madrid, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Rivas
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046, Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Mingorance
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046, Madrid, Spain. Electronic address: mromerogomez@salud.madrid.org.
authors.forename: María Pilar
authors.initials: MP
authors.lastname: Romero-Gómez
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000150:complications*; D008297:Male; D008828:Microbiological Techniques / Q000379:methods*; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis*; D013997:Time Factors; D055815:Young Adult
keywords: Multiplex PCR for diagnostic of sepsis in kidney patients