title: Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas.
abstract: An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B. abortus-B. melitensis-B. ovis-B. suis (AMOS)-PCR. Unexpectedly, the isolate showed a phenotype different from B. abortus bv 1, the most prevalent strain in cattle in Argentina, and from vaccine strain 19, currently used in bovines and water buffaloes. Genotyping supported the phenotypic results, as the analysis of the omp2ab gene sequence showed an identical pattern to either B. abortus bv 5 or B. melitensis. Finally, the AMOS PCR generated a 1700-bp fragment from the isolate, different than those amplified from B. abortus bv 1 (498bp) and B. melitensis (731bp), confirming the presence of B. abortus bv 5. The OIE/FAO Reference Laboratory for Brucellosis confirmed this typing. This is the first report of B. abortus bv 5 from a water buffalo in the Americas.
authors.affiliation: Universidad Nacional del Nordeste, Facultad de Ciencias Veterinarias, Sargento Cabral 2139 - (3400) Corrientes, Argentina.
authors.forename: Diana
authors.initials: D
authors.lastname: Martínez
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela, Ruta 34km 227, CP 2300 Rafaela, Santa Fe, Argentina.
authors.forename: Carolina
authors.initials: C
authors.lastname: Thompson
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Mercedes, Juan Pujol Este s/n, CP 3470 Mercedes, Corrientes, Argentina.
authors.forename: Graciela
authors.initials: G
authors.lastname: Draghi
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela, Ruta 34km 227, CP 2300 Rafaela, Santa Fe, Argentina.
authors.forename: Vilma
authors.initials: V
authors.lastname: Canavesio
authors.affiliation: Universidad Nacional del Nordeste, Facultad de Ciencias Veterinarias, Sargento Cabral 2139 - (3400) Corrientes, Argentina.
authors.forename: Roberto
authors.initials: R
authors.lastname: Jacobo
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Mercedes, Juan Pujol Este s/n, CP 3470 Mercedes, Corrientes, Argentina.
authors.forename: Patricia
authors.initials: P
authors.lastname: Zimmer
authors.affiliation: Laboratorio y Centro de Referencia de la OIE y FAO para Brucelosis (DILAB-SENASA) Talcahuano 1660, 1640 Martinez, Buenos Aires, Argentina.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Elena
authors.affiliation: Laboratorio y Centro de Referencia de la OIE y FAO para Brucelosis (DILAB-SENASA) Talcahuano 1660, 1640 Martinez, Buenos Aires, Argentina.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Nicola
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela, Ruta 34km 227, CP 2300 Rafaela, Santa Fe, Argentina. Electronic address: torioni.susana@inta.gob.ar.
authors.forename: Susana Torioni
authors.initials: ST
authors.lastname: de  Echaide
mesh_terms: D000818:Animals; D001118:Argentina; D015373:Bacterial Typing Techniques; D002003:Brucella abortus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002020:Buffaloes / Q000382:microbiology*; D005333:Fetus; D005838:Genotype; D007106:Immune Sera / Q000276:immunology; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D007106:Immune Sera
keywords: Biovar 5; Brucella abortus; Bubalus bubalis; PCR; Typing