title: Interaction of Serum microRNAs and Serum Folate With the Susceptibility to Pancreatic Cancer.
abstract: OBJECTIVE
  The aim of this study was to investigate whether 6 candidate serum miRNAs and their interactions with serum folate level were associated with the risk for pancreatic cancer (PC).
  
  
  METHODS
  A hospital-based case-control study including 74 incident PC cases and 74 controls was conducted. Serum folate and miRNAs were determined by radioimmunoassay and real-time quantitative polymerase chain reaction, respectively. Cell lines AsPC-1 and PANC-1 were used for in vitro study.
  
  
  RESULTS
  MiR-16 was elevated (P = 0.030-0.043) and miR-103 was reduced (P = 0.018-0.020) in PC after adjustment for age, sex, and smoking; however, after additional adjustment for folate, only miR-103 was significantly different between cases and controls (P = 0.010). After converting the relative expression of miRNAs into binary variables and adjusting for age, sex, smoking, and folate, the subjects with low miR-103 or low miR-601 were observed to have a higher risk for PC, with odds ratios of 2.33 (95% confidence interval, 1.06-5.10) and 2.37 (95% confidence interval, 1.07-5.26), respectively. Multifactor dimensionality reduction analysis showed a significant interaction for miR-16, folate, and smoking (cross-validation consistency, 10/10; mean testing accuracy, 0.696; P = 0.013). Interaction between miR-16 and folate was also verified in the AsPC-1 cells.
  
  
  CONCLUSIONS
  Serum miR-103; miR-601; and interactions among serum miR-16, folate, and smoking are associated with PC.
authors.affiliation: From the *Department of Epidemiology and Biostatistics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences; †School of Basic Medicine, Peking Union Medical College; ‡Division of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences; and §Peking Union Medical College, Beijing, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Tian
authors.forename: Yibo
authors.initials: Y
authors.lastname: Xue
authors.forename: Gechong
authors.initials: G
authors.lastname: Ruan
authors.forename: Kailiang
authors.initials: K
authors.lastname: Cheng
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.forename: Qian
authors.initials: Q
authors.lastname: Qiu
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D002681:China / Q000453:epidemiology; D005260:Female; D005492:Folic Acid / Q000097:blood*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000453:epidemiology / Q000235:genetics; D011237:Predictive Value of Tests; D011379:Prognosis; D011863:Radioimmunoassay; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
chemical_list: D014408:Biomarkers, Tumor; C576906:MIRN1228 microRNA, human; C572916:MIRN320 microRNA, human; C580741:MIRN601 microRNA, human; D035683:MicroRNAs; D005492:Folic Acid
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