title: Vascular programming in twins: the effects of chorionicity and fetal therapy for twin-to-twin transfusion syndrome.
abstract: We assessed vascular programming in genetically identical monochorionic twin pairs with twin-to-twin transfusion syndrome (TTTS) treated differently in utero by serial amnioreduction or fetal laser arterial photocoagulation. This case-control study re-assessed four twin groups at median 11 years comprising 20 pairs of monochorionic diamniotic twins: nine treated by amnioreduction (TTTS-amnio) and eleven by laser (TTTS-laser) with seven monochorionic and six dichorionic control pairs. Outcome measures were current blood pressure (BP), brachio-radial arterial stiffness derived from pulse wave velocity (PWV), resting microcirculation (Flux) and response to heating and post-occlusive reactive hyperaemia measured using laser Doppler. Potential confounders [PWV and BP at first study, current height, weight, heart rate and twin type (ex-recipient, ex-donor or heavier/lighter of pair)] were accounted for by Mixed Linear Models statistical methodology. PWV dichorionic > monochorionic (P = 0.024); systolic and diastolic BP dichorionic > TTTS-amnio and TTTS-laser (P = 0.004, P = 0.02 and P = 0.005, P = 0.02, respectively). Within-twin pair pattern of PWV discordance was similar in laser treated and dichorionic controls (heavier-born > lighter), opposite to TTTS-amnio and monochorionic controls. Flux monochorionic > dichorionic (P = 0.044) and heavier > lighter-born (P = 0.024). TTTS-laser and dichorionic diamniotic showed greatest hyperaemic responses (dichorionic > TTTS-amnio or monochorionic controls (P = 0.007, P = 0.025). Hyperaemic responses were slower in heavier-born twins (P = 0.005). In summary, monochorionic twins had lower BP, arterial stiffness and increased resting vasodilatation than dichorionic twins implying shared fetal circulation affects vascular development. Vascular responses in laser-TTTS were similar to dichorionic and opposite to TTTS-amnio suggesting a lasting effect of fetal therapy on vascular health.
authors.affiliation: 1Faculty of Medicine, Imperial College, Queen Charlotte's and Chelsea Hospital, London, UK.
authors.forename: H M
authors.initials: HM
authors.lastname: Gardiner
authors.affiliation: 3Blizard Institute of Cell and Molecular Science, Barts and The London School of Medicine and Dentistry, London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Barlas
authors.affiliation: 1Faculty of Medicine, Imperial College, Queen Charlotte's and Chelsea Hospital, London, UK.
authors.forename: H
authors.initials: H
authors.lastname: Matsui
authors.affiliation: 4Department of Obstetrics and Fetal Medicine, University Medical Center, Hamburg-Eppendorf, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Diemert
authors.affiliation: 1Faculty of Medicine, Imperial College, Queen Charlotte's and Chelsea Hospital, London, UK.
authors.forename: M J O
authors.initials: MJ
authors.lastname: Taylor
authors.affiliation: 1Faculty of Medicine, Imperial College, Queen Charlotte's and Chelsea Hospital, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Preece
authors.affiliation: 5Statistical Advisory Service, Imperial College, London, UK.
authors.forename: F
authors.initials: F
authors.lastname: Gordon
authors.affiliation: 3Blizard Institute of Cell and Molecular Science, Barts and The London School of Medicine and Dentistry, London, UK.
authors.forename: S E
authors.initials: SE
authors.lastname: Greenwald
authors.affiliation: 4Department of Obstetrics and Fetal Medicine, University Medical Center, Hamburg-Eppendorf, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Hecher