title: Oral supplementation of Ocimum basilicum has the potential to improves the locomotory, exploratory, anxiolytic behavior and learning in adult male albino mice.
abstract: The aim of this project was to determine the effect of 100 mg/ml solvent/kg body weight of Ocimum basilicum leaf extract on neuromuscular co-ordination, exploratory, locomotory and short-term memory formation in male albino mice. Five weeks old, male albino mice were used as the experimental animals in order to demonstrate the effect of O. basilicum's extract on learning and memory. Each male albino mouse was weighted and orally treated either with 100 mg/ml solvent/kg body weight of O. basilicum leaf extract or with commercially available saline solution (Otsuka, Pakistan) for 7 days. Behavioral observations were made by applying a series of neurological tests (Elevated plus maze, Light and dark box, Open field and Rota rod). Dose supplementation continued during neurological testing. It was observed that 100 mg/ml solvent/kg body weight of leaf extract improves neuromuscular co-ordination and male albino mouse performance in open field, light dark box and during novel object test when compared with control group. We concluded that 100 mg/ml solvent/kg body weight of leaf extract has the potential to improve neuromuscular co-ordination, exploratory behavior, object recognition ability and transfer latency in male albino mice and can be safely administrated orally.
authors.affiliation: Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60800, Pakistan.
authors.forename: K
authors.initials: K
authors.lastname: Zahra
authors.forename: M A
authors.initials: MA
authors.lastname: Khan
authors.forename: F
authors.initials: F
authors.lastname: Iqbal
mesh_terms: D000284:Administration, Oral; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D001007:Anxiety / Q000188:drug therapy*; D005106:Exploratory Behavior / Q000187:drug effects*; D007858:Learning / Q000187:drug effects*; D008297:Male; D008570:Memory, Short-Term / Q000187:drug effects; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000187:drug effects*; D018649:Ocimum basilicum; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D045442:Rotarod Performance Test; D014163:Transfer, Psychology / Q000187:drug effects
chemical_list: D014151:Anti-Anxiety Agents; D010936:Plant Extracts
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