title: Comparative gene array analysis of progenitor cells from human paired deep neck and subcutaneous adipose tissue.
abstract: Brown and white adipocytes have been shown to derive from different progenitors. In this study we sought to clarify the molecular differences between human brown and white adipocyte progenitors cells. To this end, we performed comparative gene array analysis on progenitor cells isolated from paired biopsies of deep and subcutaneous neck adipose tissue from individuals (n = 6) undergoing neck surgery. Compared with subcutaneous neck progenitors, cells from the deep neck adipose tissue displayed marked differences in gene expression pattern, including 355 differentially regulated (>1.5 fold) genes. Analysis of highest regulated genes revealed that STMN2, MME, ODZ2, NRN1 and IL13RA2 genes were specifically expressed in white progenitor cells, whereas expression of LRRC17, CNTNAP3, CD34, RGS7BP and ADH1B marked brown progenitor cells. In conclusion, progenitors from deep neck and subcutaneous neck adipose tissue are characterized by a distinct molecular signature, giving rise to either brown or white adipocytes. The newly identified markers may provide potential pharmacological targets facilitating brown adipogenesis.
authors.affiliation: Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Tews
authors.affiliation: Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Schwar
authors.affiliation: Clinic of Otorhinolaryngology, University Medical Center Ulm, Ulm, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Scheithauer
authors.affiliation: Department of Surgery, University Medical Center Ulm, Ulm, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Weber
authors.affiliation: Else-Kröner-Fresenius Center for Nutritional Medicine, Technische Universität München, Munich, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Fromme
authors.affiliation: Else-Kröner-Fresenius Center for Nutritional Medicine, Technische Universität München, Munich, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Klingenspor
authors.affiliation: Department of Pathology, University Medical Center Ulm, Ulm, Germany.
authors.forename: T F
authors.initials: TF
authors.lastname: Barth
authors.affiliation: Department of Pathology, University Medical Center Ulm, Ulm, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Möller
authors.affiliation: Core Facility Genomics, Ulm University, Ulm, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Holzmann
authors.affiliation: Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany.
authors.forename: K M
authors.initials: KM
authors.lastname: Debatin
authors.affiliation: Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Fischer-Posovszky
authors.affiliation: Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany. Electronic address: martin.wabitsch@uniklinik-ulm.de.
authors.forename: M
authors.initials: M
authors.lastname: Wabitsch
mesh_terms: D052437:Adipocytes, Brown / Q000166:cytology / Q000378:metabolism*; D052438:Adipocytes, White / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000502:physiology*; D000328:Adult; D000368:Aged; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009333:Neck; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism*
keywords: Adipogenesis; Brown adipose tissue; Obesity; Progenitor cells