title: Are endothelial outgrowth cells a potential source for future re-vascularization therapy?
abstract: Endothelial progenitor cells (EPCs) represent a heterogeneous cell population that is believed to be involved in vasculogenesis after ischemic diseases. EPCs could have a potential for future therapies with the purpose of enhancing endothelial repair. However, due to the low amount of these cells in circulation they have to be expanded in vitro before administration into recipients. The purpose of this study was to analyse and evaluate possible changes in morphology and functionality as a result of in vitro ageing of a subtype of EPCs called endothelial outgrowth cells (EOCs), since such changes might compromise the cells' ability to participate in vasculogenesis. EOCs were isolated and grown from human umbilical cord blood using two methodologies with varying degree of cell purification. The changes between the two culture setups and the changes occurring in EOCs over time were traced by flow cytometry and assays for growth, tube formation, and beta-galactosidase production. The cells showed to be indistinguishable from each other during the first weeks of culture. The cells showed a changed morphology with bigger and more granular cells and the growth rate decreased with time. The cells also showed an increased Beta-galactosidase expression and decreased tube formation ability in late passage EOCs. Our data indicates that EOCs undergo senescence during long-term expansion and therefore time for cell harvest has to be validated in order to achieve functional cells still maintaining a therapeutic potential. A possible application in large animal or humans could be local injection of EOCs into affected areas and thereby reducing the need for long-term expansion of the cells.
authors.affiliation: The MR Research Centre, Department of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark. Electronic address: lotte@mr.au.dk.
authors.forename: Lotte B
authors.initials: LB
authors.lastname: Bertelsen
authors.affiliation: Department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Anja B
authors.initials: AB
authors.lastname: Bohn
authors.affiliation: Aarhus School of Engineering, Aarhus University, Aarhus, Denmark.
authors.forename: Màiri
authors.initials: M
authors.lastname: Smith
authors.affiliation: Aarhus School of Engineering, Aarhus University, Aarhus, Denmark.
authors.forename: Brian
authors.initials: B
authors.lastname: Mølgaard
authors.affiliation: Department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Bjarne
authors.initials: B
authors.lastname: Møller
authors.affiliation: The MR Research Centre, Department of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Hans
authors.initials: H
authors.lastname: Stødkilde-Jørgensen
authors.affiliation: Department of Engineering, Aarhus University, Aarhus, Denmark.
authors.forename: Peter
authors.initials: P
authors.lastname: Kristensen
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D002478:Cells, Cultured; D016922:Cellular Senescence; D066026:Endothelial Progenitor Cells / Q000502:physiology* / Q000637:transplantation*; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D018919:Neovascularization, Physiologic; D010641:Phenotype; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D001616:beta-Galactosidase
keywords: Cell culture; Endothelial outgrowth cells; Senescence