title: Regulation of Human PAX6 Expression by miR-7.
abstract: The paired box gene 6 (PAX6) is a powerful mediator of eye and brain organogenesis whose spatiotemporal expression is exquisitely controlled by multiple mechanisms, including post-transcriptional regulation by microRNAs (miRNAs). In the present study, we use bioinformatic predictions to identify three candidate microRNA-7 (miR-7) target sites in the human PAX6 3' untranslated region (3'-UTR) and demonstrate that two of them are functionally active in a human cell line. Furthermore, transient transfection of cells with synthetic miR-7 inhibits PAX6 protein expression but does not alter levels of PAX6 mRNA, suggesting that miR-7 induces translational repression of PAX6. Finally, a comparison of PAX6 3'-UTRs across species reveals that one of the functional miR-7 target sites is conserved, whereas the second functional target site is found only in primates. Thus, the interaction between PAX6 and miR-7 appears to be highly conserved; however, the precise number of sites through which this interaction occurs may have expanded throughout evolution.
authors.affiliation: Parkinson's Centre (ParkC), School of Medical Sciences, Edith Cowan University, Joondalup, Western Australia, Australia. ; Experimental and Regenerative Neurosciences (EaRN), School of Animal Biology, University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Maria
authors.initials: M
authors.lastname: Needhamsen
authors.affiliation: Parkinson's Centre (ParkC), School of Medical Sciences, Edith Cowan University, Joondalup, Western Australia, Australia. ; Experimental and Regenerative Neurosciences (EaRN), School of Animal Biology, University of Western Australia, Crawley, Western Australia, Australia. ; School of Anatomy, Physiology and Human Biology, University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Robert B
authors.initials: RB
authors.lastname: White
authors.affiliation: Harry Perkins Institute of Medical Research, Nedlands, Western Australia, Australia.
authors.forename: Keith M
authors.initials: KM
authors.lastname: Giles
authors.affiliation: Experimental and Regenerative Neurosciences (EaRN), School of Animal Biology, University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Dunlop
authors.affiliation: Parkinson's Centre (ParkC), School of Medical Sciences, Edith Cowan University, Joondalup, Western Australia, Australia. ; Experimental and Regenerative Neurosciences (EaRN), School of Animal Biology, University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Meghan G
authors.initials: MG
authors.lastname: Thomas
keywords: 3′-UTR; PAX6; miR-7; miRNA
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