title: Jasmonate-induced biosynthesis of andrographolide in Andrographis paniculata.
abstract: Andrographolide is a prominent secondary metabolite found in Andrographis paniculata that exhibits enormous pharmacological effects. In spite of immense value, the normal biosynthesis of andrographolide results in low amount of the metabolite. To induce the biosynthesis of andrographolide, we attempted elicitor-induced activation of andrographolide biosynthesis in cell cultures of A. paniculata. This was carried out by using methyl jasmonate (MeJA) as an elicitor. Among the various concentrations of MeJA tested at different time periods, 5 µM MeJA yielded 5.25 times more andrographolide content after 24 h of treatment. The accumulation of andrographolide was correlated with the expression level of known regulatory genes (hmgs, hmgr, dxs, dxr, isph and ggps) of mevalonic acid (MVA) and 2-C-methyl-d-erythritol-4-phosphate (MEP) pathways. These results established the involvement of MeJA in andrographolide biosynthesis by inducing the transcription of its biosynthetic pathways genes. The coordination of isph, ggps and hmgs expression highly influenced the andrographolide biosynthesis.
authors.affiliation: Department of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, 492012, India.
authors.forename: Shiv Narayan
authors.initials: SN
authors.lastname: Sharma
authors.forename: Zenu
authors.initials: Z
authors.lastname: Jha
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Sinha
authors.forename: Arvind Kumar
authors.initials: AK
authors.lastname: Geda
mesh_terms: D000085:Acetates / Q000494:pharmacology; D035685:Andrographis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000187:drug effects; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D054883:Oxylipins / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D004224:Diterpenes; D054883:Oxylipins; C030419:andrographolide; C011006:jasmonic acid; C072239:methyl jasmonate