title: Transient Darwinian selection in Salmonella enterica serovar Paratyphi A during 450 years of global spread of enteric fever.
abstract: Multiple epidemic diseases have been designated as emerging or reemerging because the numbers of clinical cases have increased. Emerging diseases are often suspected to be driven by increased virulence or fitness, possibly associated with the gain of novel genes or mutations. However, the time period over which humans have been afflicted by such diseases is only known for very few bacterial pathogens, and the evidence for recently increased virulence or fitness is scanty. Has Darwinian (diversifying) selection at the genomic level recently driven microevolution within bacterial pathogens of humans? Salmonella enterica serovar Paratyphi A is a major cause of enteric fever, with a microbiological history dating to 1898. We identified seven modern lineages among 149 genomes on the basis of 4,584 SNPs in the core genome and estimated that Paratyphi A originated 450 y ago. During that time period, the effective population size has undergone expansion, reduction, and recent expansion. Mutations, some of which inactivate genes, have occurred continuously over the history of Paratyphi A, as has the gain or loss of accessory genes. We also identified 273 mutations that were under Darwinian selection. However, most genetic changes are transient, continuously being removed by purifying selection, and the genome of Paratyphi A has not changed dramatically over centuries. We conclude that Darwinian selection is not responsible for increased frequency of enteric fever and suggest that environmental changes may be more important for the frequency of disease.
authors.affiliation: Warwick Medical School, University of Warwick, Coventry CV4 7AL, United Kingdom;Environmental Research Institute and Department of Microbiology, University College Cork, Cork, Ireland; zhemin.zhou@warwick.ac.uk m.achtman@warwick.ac.uk.
authors.forename: Zhemin
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Environmental Research Institute and Department of Microbiology, University College Cork, Cork, Ireland;
authors.forename: Angela
authors.initials: A
authors.lastname: McCann
authors.affiliation: Institut Pasteur, Unité des Bactéries Pathogènes Entériques, 75724 Paris, France;
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Weill
authors.affiliation: Environmental Research Institute and Department of Microbiology, University College Cork, Cork, Ireland;
authors.forename: Camille
authors.initials: C
authors.lastname: Blin
authors.affiliation: Salmonella Reference Service, Public Health England, Colindale, London NW9 5EQ, United Kingdom; and.
authors.forename: Satheesh
authors.initials: S
authors.lastname: Nair
authors.affiliation: Salmonella Reference Service, Public Health England, Colindale, London NW9 5EQ, United Kingdom; andWellcome Trust Genome Campus, The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom.
authors.forename: John
authors.initials: J
authors.lastname: Wain
authors.affiliation: Wellcome Trust Genome Campus, The Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom.
authors.forename: Gordon
authors.initials: G
authors.lastname: Dougan
authors.affiliation: Warwick Medical School, University of Warwick, Coventry CV4 7AL, United Kingdom;Environmental Research Institute and Department of Microbiology, University College Cork, Cork, Ireland; zhemin.zhou@warwick.ac.uk m.achtman@warwick.ac.uk.
authors.forename: Mark
authors.initials: M
authors.lastname: Achtman
mesh_terms: D005798:Genes, Bacterial; D014943:Global Health; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D019779:Salmonella enterica / Q000235:genetics*; D012641:Selection, Genetic; D014435:Typhoid Fever / Q000453:epidemiology* / Q000382:microbiology
keywords: historical reconstruction; pathogen evolution; phylogeography
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