title: Molecular cloning and functional characterization of the NFIL3/E4BP4 transcription factor of grass carp, Ctenopharyngodon idella.
abstract: NFIL3 (nuclear factor interleukin 3-regulated) is an important bZIP transcription factor in the immune response and immune cells' development. Here, we identified the NFIL3 gene from grass carp (Ctenopharyngodon idella; gcNFIL3). The deduced amino acid sequence of gcNFIL3 is 468 residues with a typical bZIP domain. Phylogenetics demonstrated that gcNFIL3 clustered closely with NFIL3 of zebrafish. Real-time PCR revealed gcNFIL3 is constitutively expressed in all tissues examined. Its expression was significantly upregulated in head kidney and trunk kidney after stimulation by bacteria. Immunofluorescence microscopy revealed that gcNFIL3 is mainly expressed in the nucleus. Overexpression of gcNFIL3 reduces Aeromonas hydrophila invasion and proliferation. In CIK cells, gcNFIL3 could induce the activation of NF-kappa B and upregulates the expression of IL10 and IFN. These results indicated that gcNFIL3 has immunoregulatory properties and might play a role in the immune response of fish.
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999 Huchenghuan Road, 201306 Shanghai, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999 Huchenghuan Road, 201306 Shanghai, China.
authors.forename: Yubang
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999 Huchenghuan Road, 201306 Shanghai, China.
authors.forename: Junlong
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999 Huchenghuan Road, 201306 Shanghai, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Key Laboratory of Conventional Freshwater Fish Breeding and Health Culture Technology Germplasm Resources, Ministry of Agriculture, Suzhou Wujiang Area Aquaculture Limited Company, 215221 Suzhou, China.
authors.forename: Rongquan
authors.initials: R
authors.lastname: Wang
authors.affiliation: Key Laboratory of Conventional Freshwater Fish Breeding and Health Culture Technology Germplasm Resources, Ministry of Agriculture, Suzhou Wujiang Area Aquaculture Limited Company, 215221 Suzhou, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Xuan
authors.affiliation: National Pathogen Collection Center for Aquatic Animals, College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, 201306 Shanghai, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Lu
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999 Huchenghuan Road, 201306 Shanghai, China; E-Institute of Shanghai Universities, Shanghai Ocean University, 999 Huchenghuan Road, 201306 Shanghai, China. Electronic address: jlli2009@126.com.
authors.forename: Jiale
authors.initials: J
authors.lastname: Li
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology; D000818:Animals; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000276:immunology*; D002347:Carps / Q000145:classification / Q000235:genetics / Q000276:immunology*; D002467:Cell Nucleus / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology*; D015870:Gene Expression; D007113:Immunity, Innate; D007372:Interferons / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology*; D007668:Kidney / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D016366:Open Reading Frames; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D015398:Signal Transduction / Q000276:immunology*; D015027:Zebrafish / Q000235:genetics / Q000276:immunology
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D029941:Fish Proteins; D016328:NF-kappa B; D016753:Interleukin-10; D007372:Interferons
keywords: Bacteria; Grass carp; Immunity; NFIL3