title: Importance of Ca(2+) in gastric epithelial restitution-new views revealed by real-time in vivo measurements.
abstract: It has been a few decades since Ca(2+) was identified as one of the important factors that can accelerate gastric wound repair as well as contribute to epithelial homeostasis and regulation of gastric secretions. The mechanistic basis has remained largely unexplored in vivo because it was not possible to track in real time either intracellular Ca(2+) mobilization or wound repair in living tissues. Recent advances in technology, such as combining high resolution light microscopy and genetically encoded Ca(2+) reporters in mice, now allow the monitoring of Ca(2+) mobilization during gastric epithelial cell restitution. Ca(2+) is a ubiquitous second messenger that influences numerous cellular processes, including gastric acid/bicarbonate secretion, mucus secretion, and cell migration. We have demonstrated that cytosolic Ca(2+) mobilization within the restituting gastric epithelial cells is a central signal driving small wound repair. However, extracellular Ca(2+) is also mobilized in the juxtamucosal luminal space above a wound, and evidence suggests extracellular Ca(2+) is a third messenger that also promotes gastric epithelial restitution. Interplay between intracellular and extracellular Ca(2+) is necessary for efficient gastric epithelial restitution.
authors.affiliation: Department of Molecular and Cellular Physiology, University of Cincinnati, OH 45267, USA.
authors.forename: Eitaro
authors.initials: E
authors.lastname: Aihara
authors.affiliation: Department of Molecular and Cellular Physiology, University of Cincinnati, OH 45267, USA. Electronic address: mhm@uc.edu.
authors.forename: Marshall H
authors.initials: MH
authors.lastname: Montrose
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D018613:Microscopy, Confocal; D014945:Wound Healing / Q000502:physiology*
chemical_list: D002118:Calcium
references.citation: Clin Cancer Res. 2008 Oct 15;14(20):6564-73
references.pmid: 18927297
references.citation: Am J Physiol Cell Physiol. 2008 Jan;294(1):C223-32
references.pmid: 17959725
references.citation: J Biol Chem. 2007 Apr 20;282(16):11613-7
references.pmid: 17329241
references.citation: Gut. 2010 Sep;59(9):1184-91
references.pmid: 20587547
references.citation: J Physiol. 2002 Nov 1;544(3):871-82
references.pmid: 12411530
references.citation: Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2895-900
references.pmid: 18287061
references.citation: J Chin Med Assoc. 2009 Oct;72(10):509-14
references.pmid: 19837644
references.citation: Am J Physiol. 1993 Sep;265(3 Pt 1):G418-22
references.pmid: 8214062
references.citation: Curr Pharm Des. 2013;19(1):126-32
references.pmid: 22950493
references.citation: Am J Physiol Gastrointest Liver Physiol. 2002 Feb;282(2):G211-9
references.pmid: 11804841
references.citation: Am J Physiol. 1995 Jun;268(6 Pt 1):G933-42
references.pmid: 7611414
references.citation: Am J Physiol Gastrointest Liver Physiol. 2001 Nov;281(5):G1151-7
references.pmid: 11668023
references.citation: Am J Physiol. 1985 Jan;248(1 Pt 1):G79-86
references.pmid: 2578256
references.citation: Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15350-5
references.pmid: 19706394
references.citation: EMBO J. 2001 Nov 15;20(22):6316-26
references.pmid: 11707403
references.citation: Am J Physiol Cell Physiol. 2002 Apr;282(4):C885-98
references.pmid: 11880277
references.citation: J Pharmacol Exp Ther. 2007 Oct;323(1):1-9
references.pmid: 17609422
references.citation: J Physiol Pharmacol. 2008 Dec;59(4):691-706
references.pmid: 19212004
references.citation: Am J Physiol Gastrointest Liver Physiol. 2004 Apr;286(4):G596-605
references.pmid: 14604862
references.citation: Dig Dis Sci. 1995 Nov;40(11):2495-6
references.pmid: 7587840
references.citation: Dig Dis Sci. 1985 Nov;30(11):1072-8
references.pmid: 2414082
references.citation: Acta Physiol Scand. 2005 Jun;184(2):151-9
references.pmid: 15916675
references.citation: Gastroenterology. 2008 Jul;135(1):41-60
references.pmid: 18549814
references.citation: Am J Physiol. 1997 Mar;272(3 Pt 1):G646-53
references.pmid: 9124587
references.citation: Biochem Pharmacol. 2007 Jan 1;73(1):103-14
references.pmid: 17069767
references.citation: J Biol Chem. 2013 Nov 22;288(47):33585-33597
references.pmid: 24121509
references.citation: Gastroenterology. 1995 Oct;109(4):1214-23
references.pmid: 7557088
references.citation: Dig Dis Sci. 2002 May;47(5):1008-14
references.pmid: 12018895
references.citation: Am J Physiol. 1982 Mar;242(3):G183-93
references.pmid: 7039351
references.citation: J Clin Invest. 2006 Apr;116(4):878-91
references.pmid: 16528408
references.citation: Am J Physiol Gastrointest Liver Physiol. 2006 Apr;290(4):G782-92
references.pmid: 16282360
references.citation: Gastroenterology. 1985 Jan;88(1 Pt 2):237-49
references.pmid: 3917262
references.citation: J Physiol. 2007 Aug 15;583(Pt 1):25-36
references.pmid: 17615095
references.citation: J Biol Chem. 2007 May 4;282(18):13477-86
references.pmid: 17363364
references.citation: Pharmacol Res. 1994 Apr;29(3):217-24
references.pmid: 8058594
references.citation: J Cell Biol. 2004 Jul 5;166(1):111-9
references.pmid: 15240573
references.citation: Pharmacol Res. 2000 Mar;41(3):265-81
references.pmid: 10675278
references.citation: J Membr Biol. 2005 Jun;205(3):129-37
references.pmid: 16362501
references.citation: Curr Top Membr. 2013;71:149-79
references.pmid: 23890115
references.citation: Digestion. 2003;67(4):186-94
references.pmid: 12966226
references.citation: Am J Physiol Gastrointest Liver Physiol. 2005 Dec;289(6):G1061-6
references.pmid: 16099867
references.citation: Dig Dis Sci. 1983 Nov;28(11):993-1000
references.pmid: 6628161
references.citation: Ann N Y Acad Sci. 2007 Mar;1099:276-86
references.pmid: 17446468
references.citation: J Biol Chem. 2007 Mar 23;282(12):9105-16
references.pmid: 17224452
references.citation: Physiology (Bethesda). 2007 Oct;22:320-7
references.pmid: 17928545
references.citation: Harvey Lect. 2003-2004;99:77-93
references.pmid: 15984552
references.citation: Gastroenterology. 1997 Nov;113(5):1553-9
references.pmid: 9352857
references.citation: Eur J Gastroenterol Hepatol. 1995 Nov;7(11):1105-22
references.pmid: 8680912
references.citation: Cell Calcium. 2005 Jan;37(1):1-8
references.pmid: 15541458
references.citation: Scand J Gastroenterol Suppl. 1984;101:87-95
references.pmid: 6336239
references.citation: Nature. 1997 Aug 28;388(6645):882-7
references.pmid: 9278050
references.citation: Dig Dis Sci. 1993 Aug;38(8):1426-34
references.pmid: 8393756
references.citation: J Biol Chem. 2008 May 9;283(19):12935-40
references.pmid: 18326500
references.citation: Cell Mol Life Sci. 2005 Dec;62(24):2932-8
references.pmid: 16374581
references.citation: Mol Nutr Food Res. 2012 May;56(5):753-60
references.pmid: 22648622
references.citation: Am J Physiol Endocrinol Metab. 2013 Apr 1;304(7):E724-33
references.pmid: 23360827
references.citation: Am J Physiol Cell Physiol. 2012 Aug 1;303(3):C308-17
references.pmid: 22592407
references.citation: Mol Cell Biochem. 2008 Jan;308(1-2):219-26
references.pmid: 17978865
references.citation: Am J Physiol Gastrointest Liver Physiol. 2010 Jul;299(1):G255-64
references.pmid: 20413716
references.citation: Am J Physiol Cell Physiol. 2001 Aug;281(2):C430-8
references.pmid: 11443042
references.citation: Dig Dis Sci. 1999 Mar;44(3):515-22
references.pmid: 10080143
references.citation: Gastroenterology. 2008 Jun;134(7):1842-60
references.pmid: 18474247
references.citation: J Pharmacol Exp Ther. 2008 Sep;326(3):889-96
references.pmid: 18550692
references.citation: Photochem Photobiol Sci. 2006 Nov;5(11):1024-30
references.pmid: 17077898
references.citation: Blood Cells Mol Dis. 2009 Sep-Oct;43(2):211-3
references.pmid: 19447651
references.citation: Biochem Pharmacol. 1999 Dec 15;58(12):1997-2002
references.pmid: 10591156