title: Protective effect of rutin on LPS-induced acute lung injury via down-regulation of MIP-2 expression and MMP-9 activation through inhibition of Akt phosphorylation.
abstract: Lipopolysaccharide (LPS), also called endotoxin, is the important pathogen of acute lung injury (ALI), which is a clinical syndrome that still lacks effective therapeutic medicine. Rutin belongs to vitamin P and possesses various beneficial effects. In this study, we investigate the potential protective effects and the mechanisms of rutin on LPS-induced ALI. Pre-administration with rutin inhibited LPS-induced arterial blood gas exchange and neutrophils infiltration in the lungs. LPS-induced expression of macrophage inflammatory protein (MIP)-2 and activation of matrix metalloproteinase (MMP)-9 were suppressed by rutin. In addition, the inhibitory concentration of rutin on phosphorylation of Akt was similar as MIP-2 expression and MMP-9 activation. In conclusion, rutin is a potential protective agent for ALI via suppressing the blood gas exchange and neutrophil infiltration. The mechanism of rutin is down-regulation of MIP-2 expression and MMP-9 activation through inhibition of Akt phosphorylation.
authors.affiliation: Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Chen
authors.affiliation: School of Health, National Taichung University of Science and Technology, Taichung, Taiwan.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Department of Anatomy, School of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Ming-Ling
authors.initials: ML
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Pharmacy, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Chien-Ying
authors.initials: CY
authors.lastname: Lee
authors.affiliation: Department of Education and Research, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Chen
authors.affiliation: Department of Neurology, Show Chwan Memorial Hospital, Changhua, Taiwan; Department of Nursing, College of Medicine & Nursing, Hung Kuang University, Taichung, Taiwan.
authors.forename: Chung-Hsin
authors.initials: CH
authors.lastname: Yeh
authors.affiliation: Department of Pediatrics, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan.
authors.forename: Pin-Ho
authors.initials: PH
authors.lastname: Pan
authors.affiliation: Medical Education Center, Kaohsiung Armed Forced General Hospital, Kaohsiung, Taiwan; Department of Ophthalmology, Kaohsiung Armed Forced General Hospital, Kaohsiung, Taiwan.
authors.forename: Chi-Ting
authors.initials: CT
authors.lastname: Horng
authors.affiliation: Department of Medicine, Kaohsiung Armed Forced General Hospital, Kaohsiung, Taiwan; Department of Medicine, Tri-Service General Hospital, Taipei, Taiwan.
authors.forename: Wu-Hsien
authors.initials: WH
authors.lastname: Kuo
authors.affiliation: Department of Pharmacology, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Pharmacy, Chung Shan Medical University Hospital, Taichung, Taiwan. Electronic address: kuanyh@csmu.edu.tw.
authors.forename: Yu-Hsiang
authors.initials: YH
authors.lastname: Kuan
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D054426:Chemokine CXCL2 / Q000378:metabolism*; D015536:Down-Regulation; D008070:Lipopolysaccharides; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D020556:Neutrophil Infiltration / Q000187:drug effects; D010766:Phosphorylation; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D012431:Rutin / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D054426:Chemokine CXCL2; C404915:Cxcl2 protein, mouse; D008070:Lipopolysaccharides; D020011:Protective Agents; D012431:Rutin; D051057:Proto-Oncogene Proteins c-akt; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse
keywords: Acute lung injury; Akt; LPS; MIP-2; MMP-9; Rutin