title: Optimization of alkaline extraction and bioactivities of polysaccharides from rhizome of Polygonatum odoratum.
abstract: The present study is to explore the optimal extraction parameters, antioxidant activity, and antimicrobial activity of alkaline soluble polysaccharides from rhizome of Polygonatum odoratum. The optimal extraction parameters were determined as the following: NaOH concentration (A) 0.3 M, temperature (B) 80 °C, ratio of NaOH to solid (C) 10-fold, and extraction time (D) 4 h, in which ratio of NaOH to solid was a key factor. The order of the factors was ratio of NaOH to solid (fold, C) > extraction temperature (°C, B) > NaOH concentration (M, A) > extraction time (h, D). The monosaccharide compositions of polysaccharides from P. odoratum were rhamnose, mannose, xylose, and arabinose with the molecular ratio of 31.78, 31.89, 11.11, and 1.00, respectively. The reducing power, the 1, 1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging rate, the hydroxyl radicals scavenging rate, and the inhibition rate to polyunsaturated fatty acid (PUFA) peroxidation of the alkaline soluble polysaccharides from P. odoratum at 1 mg/mL were 9.81%, 52.84%, 19.22%, and 19.42% of ascorbic acid at the same concentration, respectively. They also showed antimicrobial activity against pathogenic bacteria Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli.
authors.affiliation: Department of Life Sciences, Hunan Institute of Humanities, Science and Technology, Loudi 417000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Life Sciences, Hunan Institute of Humanities, Science and Technology, Loudi 417000, China.
authors.forename: Luoyi
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Life Sciences, Hunan Institute of Humanities, Science and Technology, Loudi 417000, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Life Sciences, Hunan Institute of Humanities, Science and Technology, Loudi 417000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Life Sciences, Hunan Institute of Humanities, Science and Technology, Loudi 417000, China.
authors.forename: Qiuzhi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Life Sciences, Hunan Institute of Humanities, Science and Technology, Loudi 417000, China.
authors.forename: Chenzhong
authors.initials: C
authors.lastname: Jin
authors.affiliation: Department of Life Sciences, Hunan Institute of Humanities, Science and Technology, Loudi 417000, China.
authors.forename: Yihong
authors.identifier: 0000-0001-9697-2450
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Crop Research Institute, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, China.
authors.forename: Jihua
authors.initials: J
authors.lastname: Wang
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001089:Arabinose / Q000737:chemistry / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008358:Mannose / Q000737:chemistry / Q000302:isolation & purification; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry; D031384:Polygonatum / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012210:Rhamnose / Q000737:chemistry / Q000302:isolation & purification; D027343:Rhizome / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011134:Polysaccharides; D017665:Hydroxyl Radical; D001089:Arabinose; D008358:Mannose; D012210:Rhamnose
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