title: Bizionia psychrotolerans sp. nov., a psychrophilic bacterium isolated from the intestine of a sea cucumber (Apostichopus japonicus).
abstract: A novel Gram-negative, non-flagellated, non-gliding, rod-shaped bacterial strain, designated PB-M7(T), was isolated from the intestine of a sea cucumber collected from Pohang, South Korea. Growth was observed at 4-30 °C (optimum, 25 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0), and with 2.0-6.0% (w/v) NaCl (optimum, 2.0%). In a phylogenetic analysis based on 16S rRNA gene sequences, strain PB-M7(T) was found to belong to the genus Bizionia and to be most closely related to Bizionia echini KMM 6177(T) (99.0% 16S rRNA gene sequence similarity), Bizionia hallyeonensis T-y7(T) (97.9%), Bizionia algoritergicola APA-1(T) (97.5%), Bizionia argentinensis JUB59(T) (97.5%) and Bizionia myxarmorum ADA-4(T) (97.1%). The predominant fatty acids of strain PB-M7(T) were identified as iso-C(15:0) (22.2%), iso-C(15:1) G (10.8%), iso-C(17:0) 3-OH (16.7%) and summed feature 3 (C(16:1) ω7c and/or C(16:1) ω6c; 11.2%). The major respiratory quinone was identified as menaquinone-6 (MK-6). The polar lipid profile of strain PB-M7(T) was found to contain phosphatidylethanolamine, an unidentified phospholipid, three unidentified aminolipids and three unidentified lipids. The DNA G + C content of strain PB-M7(T) was determined to be 33.4 mol% and the mean DNA-DNA relatedness values with the type strains of B. echini, B. hallyeonensis, B. algoritergicola, B. argentinensis, and B. myxarmorum were 52.9, 48.5, 46.5, 37.1 and 26.6%, respectively. Based on the data presented, strain PB-M7(T) represents a novel species of the genus Bizionia, for which the name Bizionia psychrotolerans sp. nov. is proposed. The type strain of B. psychrotolerans is PB-M7(T) (= KCCM 43042 (T) = JCM 19924 (T)).
authors.affiliation: Fermentation Research Center, Korea Food Research Institute (KFRI), Seongnam, 463-746, Republic of Korea.
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Song
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Lee
authors.forename: Myung-Ji
authors.initials: MJ
authors.lastname: Seo
authors.forename: Kyung June
authors.initials: KJ
authors.lastname: Yim
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Hyun
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Bae
authors.forename: So-lim
authors.initials: SL
authors.lastname: Park
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.forename: Young-Do
authors.initials: YD
authors.lastname: Nam
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012616:Sea Cucumbers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride