title: Assembly principles of a unique cage formed by hexameric and decameric E. coli proteins.
abstract: A 3.3 MDa macromolecular cage between two Escherichia coli proteins with seemingly incompatible symmetries-the hexameric AAA+ ATPase RavA and the decameric inducible lysine decarboxylase LdcI-is reconstructed by cryo-electron microscopy to 11 Å resolution. Combined with a 7.5 Å resolution reconstruction of the minimal complex between LdcI and the LdcI-binding domain of RavA, and the previously solved crystal structures of the individual components, this work enables to build a reliable pseudoatomic model of this unusual architecture and to identify conformational rearrangements and specific elements essential for complex formation. The design of the cage created via lateral interactions between five RavA rings is unique for the diverse AAA+ ATPase superfamily.
authors.affiliation: European Molecular Biology Laboratory, Grenoble, France Unit for Virus Host-Cell Interactions, Université Grenoble Alpes, Grenoble, France Unit for Virus Host-Cell Interactions, CNRS, Grenoble, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Malet
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, Canada.
authors.forename: Kaiyin
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, Canada.
authors.forename: Majida
authors.initials: M
authors.lastname: El Bakkouri
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, Canada.
authors.forename: Sze Wah Samuel
authors.initials: SW
authors.lastname: Chan
authors.affiliation: Unit for Virus Host-Cell Interactions, Université Grenoble Alpes, Grenoble, France Unit for Virus Host-Cell Interactions, CNRS, Grenoble, France.
authors.forename: Gregory
authors.initials: G
authors.lastname: Effantin
authors.affiliation: Université Grenoble Alpes, Institut de Biologie Structurale, Grenoble, France Institut de Biologie Structurale, CNRS, Grenoble, France Institut de Biologie Structurale, CEA, Grenoble, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Bacia
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, Canada walid.houry@utoronto.ca.
authors.forename: Walid A
authors.initials: WA
authors.lastname: Houry
authors.affiliation: Unit for Virus Host-Cell Interactions, Université Grenoble Alpes, Grenoble, France Unit for Virus Host-Cell Interactions, CNRS, Grenoble, France gutsche@embl.fr.
authors.forename: Irina
authors.initials: I
authors.lastname: Gutsche
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002262:Carboxy-Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020285:Cryoelectron Microscopy; D004926:Escherichia coli / Q000737:chemistry* / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008958:Models, Molecular; D009154:Mutation; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D000251:Adenosine Triphosphatases; C508688:RavA protein, E coli; D002262:Carboxy-Lyases; C021520:lysine decarboxylase
keywords: AAA+ ATPase; RavA; electron microscopy; image analysis; lysine decarboxylase; macromolecular assembly
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