title: Cartilage polysaccharide induces apoptosis in K562 cells through a reactive oxygen species-mediated caspase pathway.
abstract: In this study, a polysaccharide (PS) was successfully extracted from porcine cartilage and its effect on chronic myeloid leukemia was examined using human K562 cells. The results of cell proliferation assays indicated that the PS inhibited cancer cell growth at different concentrations. Morphological and biochemical changes characteristic of apoptosis were observed and confirmed by PI staining and TUNEL assay. The nuclear DNA, RNA and proteins of the cancer cells subjected to PS treatment were irreversibly destroyed by reactive oxygen species (ROS), additionally, the ROS effected on the cells directly. The apoptotic signals altered the permeability of the mitochondrial outer membrane, thereby resulted in the release of apoptotic factors into the cytoplasm that induced apoptosis. As caspase-3/7, 8 and 9 were expressed, it was speculated that both intrinsic and extrinsic pathways were involved in the PS-induced apoptosis.
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. weisong@hit.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Panpan
authors.initials: P
authors.lastname: Hu
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Shan
authors.forename: Ming
authors.initials: M
authors.lastname: Du
authors.forename: Anjun
authors.initials: A
authors.lastname: Liu
authors.forename: Ran
authors.initials: R
authors.lastname: Ye
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002356:Cartilage / Q000737:chemistry*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053179:Caspase 7 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D020287:In Situ Nick-End Labeling; D020014:K562 Cells; D011134:Polysaccharides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine
chemical_list: D011134:Polysaccharides; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; C505407:CASP3 protein, human; C505520:CASP7 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053179:Caspase 7; D053453:Caspase 9