title: Oxidative stress participates in age-related changes in rat lumbar intervertebral discs.
abstract: Aging is a major factor associated with lumber intervertebral disc degeneration, and oxidative stress is known to play an essential role in the pathogenesis of many age-related diseases. In this study, we investigated oxidative stress in intervertebral discs of Wistar rats in three different age groups: youth, adult, and geriatric. Age-related intervertebral disc changes were examined by histological analysis. In addition, oxidative stress was evaluated by assessing nitric oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA), and advanced oxidation protein products (AOPPs). Intervertebral disc, but not serum, NO concentrations significantly differed between the three groups. Serum and intervertebral disc SOD activity gradually decreased with age. Furthermore, both serum and intervertebral disc MDA and AOPP levels gradually increased with age. Our studies suggest that oxidative stress is associated with age-related intervertebral disc changes.
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Hou
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. Electronic address: luhuading@gmail.com.
authors.forename: Huading
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Plastic Surgery, Guangzhou First Municipal People's Hospital, Guangzhou, China.
authors.forename: Mingjuan
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.affiliation: Department of Orthopedics, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000293:Adolescent; D000328:Adult; D062107:Advanced Oxidation Protein Products / Q000097:blood; D000375:Aging / Q000473:pathology / Q000502:physiology*; D000818:Animals; D006801:Humans; D007403:Intervertebral Disc / Q000378:metabolism / Q000473:pathology*; D008161:Lumbosacral Region; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D062107:Advanced Oxidation Protein Products; D009569:Nitric Oxide; D008315:Malondialdehyde; D013482:Superoxide Dismutase
keywords: Aging; Intervertebral disc; Malondialdehyde; Nitrogen monoxide; Oxidative stress; Superoxide dismutase