title: [Study on quality control method of suanzaoren hehuan formula and fingerprint of its saponins parts].
abstract: OBJECTIVE
  To establish the method for determining total saponins and paeoniflorin in Suanzaoren Hehuan Formula (SHF) and an HPLC fingerprint of its saponins parts.
  
  
  METHODS
  The total saponins in SHF was determined by ultraviolet spectrophotometry at 545 nm. The content of paeoniflorin and the chromatographic fingerprints were determined by HPLC.
  
  
  RESULTS
  There was a good linear relationship in the range of 2.3-11.8 microg/mL for oleanolic acid (r1 = 0.9997) of UV, and 12.8-38.4 pg/mL for paeoniflorin (r2 = 0.9996) of HPLC. The average recovery of oleanolic acid and paeoniflorin was 99.60% and 99.98%, respectively. Thirteen peaks were selected as common peaks in fingerprint and four of them were recognized.
  
  
  CONCLUSIONS
  The method is accurate, sensitive, rapid and suitable for quality control of this formula.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Qing-wei
authors.initials: QW
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Fei
authors.initials: F
authors.lastname: Meng
authors.forename: Wei
authors.initials: W
authors.lastname: Qiao
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
mesh_terms: D000928:Antidepressive Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis; D039821:Monoterpenes / Q000032:analysis; D009828:Oleanolic Acid / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D006027:Glycosides
chemical_list: D000928:Antidepressive Agents; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; D009828:Oleanolic Acid; D012503:Saponins; C015423:peoniflorin; D006027:Glycosides; C530157:suanzaoren