title: Effect of glyphosate on the sperm quality of zebrafish Danio rerio.
abstract: Glyphosate is a systemic, non-selective herbicide widely used in agriculture worldwide. It acts as an inhibitor of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase by interrupting the synthesis of essential aromatic amino acids. This pathway is not present in animals, although some studies have shown that the herbicide glyphosate can affect fish reproduction. In this study, the effect of glyphosate on sperm quality of the fish Danio rerio was investigated after 24 and 96 h of exposure at concentrations of 5mg/L and 10mg/L. The spermatic cell concentration, sperm motility and motility period were measured employing conventional microscopy. The mitochondrial functionality, membrane integrity and DNA integrity were measured by fluorescence microscopy using specific probes. No significant differences in sperm concentration were observed; however, sperm motility and the motility period were reduced after exposure to both glyphosate concentrations during both exposure periods. The mitochondrial functionality and membrane and DNA integrity were also reduced at the highest concentration during both exposure periods. The results showed that glyphosate can induce harmful effects on reproductive parameters in D. rerio and that this change would reduce the fertility rate of these animals.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900 Rio Grande, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, Brazil. Electronic address: fernandalopes@furg.br.
authors.forename: Fernanda Moreira
authors.initials: FM
authors.lastname: Lopes
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900 Rio Grande, RS, Brazil; Programa de Pós-Graduação em Biologia de Ambientes Aquáticos Continentais, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900 Rio Grande, RS, Brazil; Programa de Pós-Graduação em Medicina Veterinária, Universidade Federal de Pelotas, Campus Universitário, Caixa Postal 354, 96001-970 Pelotas, RS, Brazil. Electronic address: antoniovarela@furg.br.
authors.forename: Antonio Sergio
authors.initials: AS
authors.lastname: Varela Junior
authors.affiliation: Programa de Pós-Graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, Brazil; Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Universitário, Caixa Postal 354, 96001-970 Pelotas, RS, Brazil; Programa de Pós-Graduação em Medicina Veterinária, Universidade Federal de Pelotas, Campus Universitário, Caixa Postal 354, 96001-970 Pelotas, RS, Brazil. Electronic address: corcinicd@gmail.com.
authors.forename: Carine Dahl
authors.initials: CD
authors.lastname: Corcini
authors.affiliation: Programa de Pós-Graduação em Medicina Veterinária, Universidade Federal de Pelotas, Campus Universitário, Caixa Postal 354, 96001-970 Pelotas, RS, Brazil. Electronic address: alecardososilva@hotmail.com.
authors.forename: Alessandra Cardoso
authors.initials: AC
authors.lastname: da Silva
authors.affiliation: Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Universitário, Caixa Postal 354, 96001-970 Pelotas, RS, Brazil. Electronic address: vitoria.guazzelli@hotmail.com.
authors.forename: Vitória Gasperin
authors.initials: VG
authors.lastname: Guazzelli
authors.affiliation: Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Universitário, Caixa Postal 354, 96001-970 Pelotas, RS, Brazil. Electronic address: georgiadacruz.tavares@gmail.com.
authors.forename: Georgia
authors.initials: G
authors.lastname: Tavares
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900 Rio Grande, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, Brazil. Electronic address: carlosrosa@furg.br.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: da Rosa
mesh_terms: D000818:Animals; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000378:metabolism / Q000633:toxicity*; D006801:Humans; D008297:Male; D012098:Reproduction / Q000187:drug effects; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000378:metabolism; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D014874:Water Pollutants, Chemical; D000097797:Glyphosate
keywords: DNA damage; Danio rerio; Herbicide; Reproduction; Sperm motility