title: HAUSP compartmentalization in chronic myeloid leukemia.
abstract: BACKGROUND
  PTEN plays an essential role in the pathogenesis of chronic myeloid leukemia. Recently, we have shown that BCR-ABL promotes PTEN nuclear exclusion, through the modulation of HAUSP activity.
  
  
  OBJECTIVE
  Here, we investigate HAUSP cellular compartmentalization in primary CML samples.
  
  
  RESULTS
  While in normal CD34 positive cells HAUSP is expressed mostly in the nucleus, in CML CD34 cells HAUSP is expressed both in the nuclear bodies and in the cytoplasm.
  
  
  CONCLUSIONS
  This observation suggests that HAUSP behaves as a shuttling protein in CML. It can bind to BCR-ABL in the cytosol, where it is phosphorylated on tyrosine residues, and it maintains the proper compartmentalization in the nuclear bodies, where it acts as part of a PML network to regulate PTEN de-ubiquitination.
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, San Luigi Hospital, Orbassano, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Morotti
authors.forename: Cristina
authors.initials: C
authors.lastname: Panuzzo
authors.forename: Sabrina
authors.initials: S
authors.lastname: Crivellaro
authors.forename: Giovanna
authors.initials: G
authors.lastname: Carrà
authors.forename: Angelo
authors.initials: A
authors.lastname: Guerrasio
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Saglio
mesh_terms: D002460:Cell Line; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D043222:Ubiquitin Thiolesterase / Q000235:genetics / Q000378:metabolism*; D000076084:Ubiquitin-Specific Peptidase 7
chemical_list: D016044:Fusion Proteins, bcr-abl; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; C464569:USP7 protein, human; D043222:Ubiquitin Thiolesterase; D000076084:Ubiquitin-Specific Peptidase 7
keywords: HAUSP; PTEN; cellular compartmentalization; chronic myeloid leukemia