title: A novel Tn3-like composite transposon harboring blaVIM-1 in Klebsiella pneumoniae spp. pneumoniae isolated from river water.
abstract: OBJECTIVE
  We present a new plasmid (pOW16C2) with a novel Tn3-like transposon harboring blaVIM-1 from a Klebsiella pneumoniae strain isolated from river water in Switzerland.
  
  
  METHODS
  Complete nucleotide sequence of pOW16C2 was obtained using a Pacific Biosciences SMRT sequencing approach and coding sequences were predicted.
  
  
  RESULTS
  The 59,228 bp sequence included a typical IncN-like backbone and a mosaic structure with blaVIM-1, aacA4, aphA15, aadA1, catB2, qnrS1, sul1, and dfrA14 conferring resistance to carbapenems and other β-lactam antibiotics, aminoglycosides, chloramphenicol, quinolones, sulfonamides, and trimethoprim, respectively. Most of these resistance genes were inserted in a class 1 integron that was embedded in a novel Tn3-like composite transposon.
  
  
  CONCLUSIONS
  IncN plasmids carrying carbapenemases are frequently isolated from K. pneumoniae strains in clinical settings. The dissemination of K. pneumoniae harboring blaVIM-1 in surface water is a cause for increased concern to public health.
authors.affiliation: 1 Vetsuisse Faculty, Institute for Food Safety and Hygiene, University of Zurich , Zurich, Switzerland .
authors.forename: Katrin
authors.initials: K
authors.lastname: Zurfluh
authors.forename: Karen A
authors.initials: KA
authors.lastname: Power
authors.forename: Jochen
authors.initials: J
authors.lastname: Klumpp
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Séamus
authors.initials: S
authors.lastname: Fanning
authors.forename: Roger
authors.initials: R
authors.lastname: Stephan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D052978:Disk Diffusion Antimicrobial Tests; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010957:Plasmids / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D013557:Switzerland; D014871:Water Microbiology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D001618:beta-Lactamases; C000602280:beta-lactamase VIM-1, Klebsiella pneumoniae