title: Arsenic modulates posttranslational S-nitrosylation and translational proteome in keratinocytes.
abstract: Arsenic is a class I human carcinogen (such as inducing skin cancer) by its prominent chemical interaction with protein thio (-SH) group. Therefore, arsenic may compromise protein S-nitrosylation by competing the -SH binding activity. In the present study, we aimed to understand the influence of arsenic on protein S-nitrosylation and the following proteomic changes. By using primary human skin keratinocyte, we found that arsenic treatment decreased the level of protein S-nitrosylation. This was coincident to the decent expressions of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS). By using LC-MS/MS, around twenty S-nitrosoproteins were detected in the biotin-switched eluent. With the interest that arsenic not only regulates posttranslational S-nitrosylation but also separately affects protein's translation expression, we performed two-dimensional gel electrophoresis and found that 8 proteins were significantly decreased during arsenic treatment. Whether these decreased proteins are the consequence of protein S-nitrosylation will be further investigated. Taken together, these results provide a finding that arsenic can deplete the binding activity of NO and therefore reduce protein S-nitrosylation.
authors.affiliation: Department of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ; Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors.forename: Bin
authors.initials: B
authors.lastname: Huang
authors.affiliation: Department of Biotechnology, College of Life Science, No. 100, Shihchuan 1st Rd., Sanming District, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors.forename: Kuo-Hao
authors.initials: KH
authors.lastname: Chiang
authors.affiliation: Department of Dermatology, Kaohsiung Medical University Hospital and Kaohsiung Medical University College of Medicine, Kaohsiung 80708, Taiwan.
authors.forename: Hsin-Su
authors.initials: HS
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, Mackay Memorial Hospital, Taipei 10449, Taiwan.
authors.forename: Ying-Lun
authors.initials: YL
authors.lastname: Chen
authors.affiliation: Departments of Laboratory Medicine, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Kaohsiung 83301, Taiwan.
authors.forename: Huey-Ling
authors.initials: HL
authors.lastname: You
authors.affiliation: Department of Biotechnology, College of Life Science, No. 100, Shihchuan 1st Rd., Sanming District, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Liao
mesh_terms: D001151:Arsenic / Q000494:pharmacology*; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D020543:Proteome; D040901:Proteomics / Q000379:methods
chemical_list: D020543:Proteome; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D001151:Arsenic
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