title: Pseudohongiella acticola sp. nov., a novel gammaproteobacterium isolated from seawater, and emended description of the genus Pseudohongiella.
abstract: A Gram-negative, aerobic, non-spore-forming and rod-shaped or ovoid bacterial strain able to move by a single polar flagellum, designated GBSW-5(T), was isolated from seawater in the East Sea, South Korea. Strain GBSW-5(T) was found to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GBSW-5(T) clustered with the type strain of Pseudohongiella spirulinae. Strain GBSW-5(T) exhibited the highest 16S rRNA gene sequence similarity value (96.4%) to the type strain of P. spirulinae, and of less than 91.0% sequence similarities to the type strains of other recognized species. Strain GBSW-5(T) was found to contain Q-8 as the predominant ubiquinone and C(18:1)ω7c and summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c) as the major fatty acids. The major polar lipids of strain GBSW-5(T) were identified as phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The DNA G+C content of strain GBSW-5(T) was determined to be 59.1 mol%. The differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain GBSW-5(T) is separated from P. spirulinae. On the basis of the data presented, strain GBSW-5(T) is considered to represent a novel species of the genus Pseudohongiella, for which the name Pseudohongiella acticola sp. nov. is proposed. The type strain is GBSW-5(T) (= KCTC 42131(T) = CECT 8627(T)). An emended description of the genus Pseudohongiella is also given.
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.forename: Ja-Min
authors.initials: JM
authors.lastname: Park
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride