title: [Influence of lipoic acid on the level of TNF-alpha in spleen homogenates].
abstract: Lipoic acid (LA) is a natural antioxidant and possess beneficial effects on oxidative stress parameters in various tissues. The aim of the study was to estimate of influence of LA on free -SH groups and TNF-alpha levels in spleen homogenates in rats during oxidative stress induced by LPS.
  
  
  METHODS
  Experiments were performed on Wistar rats devided into 4 groups: group I (control) received 0,9% NaCl; group II --received LA (100 mg/kg); group III received LPS (10 mg/kg); group IV--received LPS (10 mg/kg) and 30 min later received LA at the dose 100 mg/kg.
  
  
  RESULTS
  Administration of LA after LPS-induced oxidative stress resulted in statistically significant increase in the level of -SH groups and decrease in the level of TNF-alpha when compared to the LPS group (p < 0.001).
  
  
  CONCLUSIONS
  The early administration of LA after LPS challenge a significant suppressed symptoms of oxidative stress and inflammatory effects LPS, expressed as a increase in -SH groups and decrease in TNF-alpha in the spleen homogenates.
authors.forename: Halina
authors.initials: H
authors.lastname: Huk-Kolega
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Ciejka
authors.forename: Beata
authors.initials: B
authors.lastname: Skibska
authors.forename: Agata
authors.initials: A
authors.lastname: Kowalczyk
authors.forename: Anna
authors.initials: A
authors.lastname: Goraca
mesh_terms: D000818:Animals; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D008063:Thioctic Acid / Q000378:metabolism* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D013438:Sulfhydryl Compounds; D014409:Tumor Necrosis Factor-alpha; D008063:Thioctic Acid