title: Au@Pt nanoparticle encapsulated target-responsive hydrogel with volumetric bar-chart chip readout for quantitative point-of-care testing.
abstract: Point-of-care testing (POCT) with the advantages of speed, simplicity, portability, and low cost is critical for the measurement of analytes in a variety of environments where access to laboratory infrastructure is lacking. While qualitative POCTs are widely available, quantitative POCTs present significant challenges. Here we describe a novel method that integrates an Au core/Pt shell nanoparticle (Au@PtNP) encapsulated target-responsive hydrogel with a volumetric bar-chart chip (V-Chip) for quantitative POCT. Upon target introduction, the hydrogel immediately dissolves and releases Au@PtNPs, which can efficiently catalyze the decomposition of H2 O2 to generate a large volume of O2 to move of an ink bar in the V-Chip. The concentration of the target introduced can be visually quantified by reading the traveling distance of the ink bar. This method has the potential to be used for portable and quantitative detection of a wide range of targets without any external instrument.
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China).
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Guan
authors.forename: Shasha
authors.initials: S
authors.lastname: Jia
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Lei
authors.forename: Shuichao
authors.initials: S
authors.lastname: Lin
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.forename: Yanli
authors.initials: Y
authors.lastname: Ma
authors.forename: Zhong-Qun
authors.initials: ZQ
authors.lastname: Tian
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D003042:Cocaine / Q000652:urine; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques; D053758:Nanoparticles / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D019095:Point-of-Care Systems
chemical_list: D052157:Aptamers, Nucleotide; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D010984:Platinum; D006046:Gold; D003042:Cocaine
keywords: aptamers; hydrogels; microfluidics; point-of-care testing