title: Snapshot of Vibrio parahaemolyticus densities in open and closed shellfish beds in Coastal South Carolina and Mississippi.
abstract: Vibrio parahaemolyticus is a Gram negative, halophilic bacterium that is ubiquitous in warm, tropical waters throughout the world. It is a major cause of seafood-associated gastroenteritis and is generally associated with consumption of raw or undercooked seafood, especially oysters. This study presents a snapshot of total V. parahaemolyticus densities in surface waters and shellstock American oysters (Crassostrea virginica) from open and closed shellfish harvesting areas, as well as "more rural areas" on two different US coasts, the Atlantic and the Gulf. Sampling was conducted from 2001 to 2003 at five sites near Charleston/Georgetown, SC and at four locations in the Gulfport/Pascagoula, MS area. V. parahaemolyticus numbers were determined by a direct plating method using an alkaline-phosphatase-labeled DNA probe targeting the species-specific thermolabile hemolysin gene (tlh) that was used for identification of bacterial isolates. The greatest difference between the two coasts was salinity; mean salinity in SC surface waters was 32.9 ppt, whereas the mean salinity in MS waters was 19.2 ppt, indicating more freshwater input into MS shellfish harvesting areas during the study period. The mean V. parahaemolyticus numbers in oysters were almost identical between the two states (567.4 vs. 560.1 CFU/g). Bacterial numbers in the majority of surface water samples from both states were at or below the limit of detection (LOD = <10 CFU/mL). The bacterial concentrations determined during this study predict a low public health risk from consumption of oysters in shellfish growing areas on either the Gulf or the Atlantic US coast.
authors.affiliation: National Oceanic and Atmospheric Administration (NOAA), National Ocean Service (NOS), National Center for Coastal Ocean Science (NCCOS), Center for Coastal Environmental Health and Biomolecular Research (CCEHBR) Lab, Charleston, SC, USA, janet.moore@noaa.gov.
authors.forename: J Gooch
authors.initials: JG
authors.lastname: Moore
authors.forename: A
authors.initials: A
authors.lastname: Ruple
authors.forename: K
authors.initials: K
authors.lastname: Ballenger-Bass
authors.forename: S
authors.initials: S
authors.lastname: Bell
authors.forename: P L
authors.initials: PL
authors.lastname: Pennington
authors.forename: G I
authors.initials: GI
authors.lastname: Scott
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D004784:Environmental Monitoring; D008922:Mississippi; D010125:Ostreidae / Q000382:microbiology; D054712:Salinity; D017747:Seafood / Q000382:microbiology; D012758:Shellfish / Q000382:microbiology*; D013022:South Carolina; D014736:Vibrio parahaemolyticus / Q000254:growth & development*; D014871:Water Microbiology
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