title: Acute and chronic toxic effects of Pb²⁺ on polychaete Perinereis aibuhitensis: morphological changes and responses of the antioxidant system.
abstract: Perinereis aibuhitensis was used to assess adverse biological effects caused by acute and chronic Pb²⁺ exposure in artificial seawater under controlled laboratory conditions. In 96-hr acute toxicity experiments, the morphological changes showed a positive time/dose-dependent tendency, and the 96-hr LC₅₀ value of Pb²⁺ was 686.41 mg/L. The responses of enzymatic and non-enzymatic antioxidants in tissues including catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde (MDA) and the content of total soluble protein (TSP), were investigated on days 1, 4, 7 and 10 after Pb²⁺ exposure under chronic toxicity testing. Results showed that the activation of the antioxidant system in P. aibuhitensis depended on the Pb²⁺ concentration and the duration of exposure time. Specifically, POD and SOD activities were induced on the first day of the exposure and decreased to the control level on day 10 after exposure. Therefore, these two indexes could be used to indicate oxidative stress associated with P. aibuhitensis exposure to Pb²⁺.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. Electronic address: tianyulu1988@gmail.com.
authors.forename: Yulu
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Marine Biology Institute of Shangdong Province, Qingdao 266002, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Liu
authors.affiliation: Marine Biology Institute of Shangdong Province, Qingdao 266002, China.
authors.forename: Qixiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Marine Biology Institute of Shangdong Province, Qingdao 266002, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. Electronic address: tangxx@ouc.edu.cn.
authors.forename: Xuexi
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000737:chemistry / Q000633:toxicity*; D007854:Lead / Q000737:chemistry / Q000633:toxicity*; D011077:Polychaeta / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D004785:Environmental Pollutants; D007854:Lead
keywords: Antioxidant system; Pb(2+); Perinereis aibuhitensis; Toxicity