title: The FinO family of bacterial RNA chaperones.
abstract: Antisense RNAs have long been known to regulate diverse aspects of plasmid biology. Here we review the FinOP system that modulates F plasmid gene expression through regulation of the F plasmid transcription factor, TraJ. FinOP is a two component system composed of an antisense RNA, FinP, which represses TraJ translation, and a protein, FinO, which is required to stabilize FinP and facilitate its interactions with its traJ mRNA target. We review the evidence that FinO acts as an RNA chaperone to bind and destabilize internal stem-loop structures within the individual RNAs that would otherwise block intermolecular RNA duplexing. Recent structural studies have provided mechanistic insights into how FinO may facilitate interactions between FinP and traJ mRNA. We also review recent findings that two other proteins, Escherichia coli ProQ and Neisseria meningitidis NMB1681, may represent FinO-like RNA chaperones.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada. Electronic address: mark.glover@ualberta.ca.
authors.forename: J N
authors.initials: JN
authors.lastname: Mark Glover
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Chaulk
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada.
authors.forename: Ross A
authors.initials: RA
authors.lastname: Edwards
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Arthur
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada.
authors.forename: Laura S
authors.initials: LS
authors.lastname: Frost
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005144:F Factor / Q000235:genetics*; D005786:Gene Expression Regulation; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D009345:Neisseria meningitidis / Q000235:genetics / Q000378:metabolism; D009690:Nucleic Acid Conformation; D016372:RNA, Antisense; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D018832:Molecular Chaperones; C118132:ProQ protein, E coli; D016372:RNA, Antisense; D012329:RNA, Bacterial; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; C041427:TraJ protein, bacteria; C074275:FinO protein, E coli
keywords: Antisense RNA; FinO; FinP; Plasmid conjugation; RNA chaperone