title: Pregnancy-secreted Acid phosphatase, uteroferrin, enhances fetal erythropoiesis.
abstract: Uteroferrin (UF) is a progesterone-induced acid phosphatase produced by uterine glandular epithelia in mammals during pregnancy and targeted to sites of hematopoiesis throughout pregnancy. The expression pattern of UF is coordinated with early fetal hematopoietic development in the yolk sac and then liver, spleen, and bone to prevent anemia in fetuses. Our previous studies suggested that UF exerts stimulatory impacts on hematopoietic progenitor cells. However, the precise role and thereby the mechanism of action of UF on hematopoiesis have not been investigated previously. Here, we report that UF is a potent regulator that can greatly enhance fetal erythropoiesis. Using primary fetal liver hematopoietic cells, we observed a synergistic stimulatory effect of UF with erythropoietin and other growth factors on both burst-forming unit-erythroid and colony-forming unit-erythroid formation. Further, we demonstrated that UF enhanced erythropoiesis at terminal stages using an in vitro culture system. Surveying genes that are crucial for erythrocyte formation at various stages revealed that UF, along with erythropoietin, up-regulated transcription factors required for terminal erythrocyte differentiation and genes required for synthesis of hemoglobin. Collectively, our results demonstrate that UF is a cytokine secreted by uterine glands in response to progesterone that promotes fetal erythropoiesis at various stages of pregnancy, including burst-forming unit-erythroid and colony-forming unit-erythroid progenitor cells and terminal stages of differentiation of hematopoietic cells in the erythroid lineage.
authors.affiliation: Department of Animal Science (W.Y., F.W.B.), Texas A&M University, College Station, Texas 77843-2471; and Department of Veterinary Physiology and Pharmacology (H.W., B.Z.), College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4466.
authors.forename: Wei
authors.initials: W
authors.lastname: Ying
authors.forename: Haiqing
authors.initials: H
authors.lastname: Wang
authors.forename: Fuller W
authors.initials: FW
authors.lastname: Bazer
authors.forename: Beiyan
authors.initials: B
authors.lastname: Zhou
mesh_terms: D000135:Acid Phosphatase / Q000494:pharmacology / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D015672:Erythroid Precursor Cells / Q000187:drug effects / Q000502:physiology; D004920:Erythropoiesis / Q000187:drug effects / Q000235:genetics*; D005260:Female; D005333:Fetus / Q000187:drug effects / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology; D007527:Isoenzymes / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D017385:Sequence Homology; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D011374:Progesterone; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
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