title: Complete mitochondrial genome of Paramisgurnus dabryanus.
abstract: In this study, the complete mitochondrial genome of Paramisgurnus dabryanus was obtained by PCR base on 18 pairs of primers. Among the 18 primers, the 14 primers were from the previously published universal primers for Cyprinus carpio L. mitogenome amplification. The remaining 4 primers were designed on the basis of related species mtDNA sequences. The genome is 16,570 bp in length, including 2 ribosomal RNA genes. 13 proteins-coding genes, 22 transfer RNA genes, and a non-coding control region, the gene composition and order of which was similar to most reported from other vertebrates. Sequence analysis showed that the overall base composition of Paramisgurnus dabryanus is T 27.3%, C 26.9%, A 28.5%, and G 17.4%. The sequence is a slight A + T bias of 55.8%, which is similar to other fishes. Mitochondrial genome is widely used in phylogenetic analysis, evolutionary genomics, species identification and related research of fish.
authors.affiliation: a Zhejiang Ocean University , Dinghai District , Zhoushan City , P. R. China.
authors.forename: Luyi
authors.initials: L
authors.lastname: Dai
authors.affiliation: a Zhejiang Ocean University , Dinghai District , Zhoushan City , P. R. China.
authors.forename: Baoying
authors.initials: B
authors.lastname: Guo
authors.affiliation: a Zhejiang Ocean University , Dinghai District , Zhoushan City , P. R. China.
authors.forename: Zhangjie
authors.initials: Z
authors.lastname: Chu
authors.affiliation: a Zhejiang Ocean University , Dinghai District , Zhoushan City , P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Zhejiang Ocean University , Dinghai District , Zhoushan City , P. R. China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
authors.affiliation: a Zhejiang Ocean University , Dinghai District , Zhoushan City , P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D018387:Codon, Initiator / Q000235:genetics; D018388:Codon, Terminator / Q000235:genetics; D003531:Cypriniformes / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D059646:Genome Size / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary*
chemical_list: D018387:Codon, Initiator; D018388:Codon, Terminator; D017931:DNA Primers; D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Mitochondrial DNA; Paramisgurnus dabryanus; sequence analysis