title: Isolation, purification, and anti-aging activity of melanin from Lachnum singerianum.
abstract: Intracellular melanin from Lachnum singerianum YM296 (LIM) was obtained by the method of microwave-assisted extraction. On the basis of single factor experiment, the optimum microwave-assisted extraction conditions of LIM using Box-Behnken design were as follows: NaOH concentration, 1.05 mol/L; ratio of raw material to liquid ratio, 1:14.72 (g/mL); microwave time, 118.70 s; and microwave power, 320 W. Under these conditions, the extraction yield of LIM was 11.08 %, and 40.43 % higher than that of extraction by alkali extraction and acid precipitation. The results showed that microwave-assisted extraction could increase the extraction yield of LIM. Three homogeneous components were fractionated from LIM by Sephadex G-15 column. LIM-a was the main homogeneous component, accounting for 75.7 % of the total content of the homogeneous components. Aged mice model were induced by injecting D-galactose, and the aged mice were given LIM-a of different dosages, respectively. The results showed that LIM-a can significantly increase body weight, thymus indexes, and spleen indexes of the aged mice; effectively elevate the levels of SOD, GSH-PX, and CAT; and decrease the level of MDA in mice liver homogenate, brain homogenate, and serum, indicating that LIM-a had significant anti-aging activity.
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.forename: Sheng
authors.initials: S
authors.lastname: Song
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.forename: Farnaz
authors.initials: F
authors.lastname: Shaikh
authors.forename: Junhong
authors.initials: J
authors.lastname: Li
mesh_terms: D000375:Aging; D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008543:Melanins / Q000302:isolation & purification* / Q000494:pharmacology*; D051379:Mice; D008872:Microwaves; D013154:Spleen / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects
chemical_list: D008543:Melanins; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase