title: Sequential expression of long noncoding RNA as mRNA gene expression in specific stages of mouse spermatogenesis.
abstract: Many long noncoding RNA (lncRNA) species have been identified in gametes. However, the biogenesis and function of other categories of lncRNAs in gametes is poorly understood. Here, we profiled the expression of lncRNAs and mRNAs in spermatogonial stem cells (SSC), type A spermatogonia (A), pachytene spermatocytes (PS) and round spermatids (RS) by microarray analysis. We analyze the total expression of lncRNA/mRNA in these four germ cells and found that the maximum number of lncRNAs expression is in A (22127), and the minimum is in PS (14456). Also, the maximum number of mRNAs is in A (19923), and the minimum is in PS (13941). Furthermore, the trend in the number of specific lncRNAs was similar to the number of specific mRNAs in each type of germ cells (e.g., maximum in A and minimum in PS). The trend in the number of lncRNAs was similar to the number of mRNAs in two continued types of germ cells (e.g., maximum in SSC to A and minimum in PS to RS). The correlation analysis showed a high correlation coefficient of lncRNAs/mRNAs expression (R = 0.992). The results suggested that the sequential expression of long noncoding RNA as mRNA gene expression exhibits coordinated changes in male spermatogenesis.
authors.affiliation: 1] International Peace Maternity &Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China [2] Department of Bioscience, Bengbu Medical College, Bengbu, Anhui 233030, China [3].
authors.forename: Meng
authors.initials: M
authors.lastname: Liang
authors.affiliation: 1] International Peace Maternity &Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China [2] School of Basic Medicine, Anhui Medical University, Hefei, Anhui 230032, China [3].
authors.forename: Wenqing
authors.initials: W
authors.lastname: Li
authors.affiliation: International Peace Maternity &Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tian
authors.affiliation: International Peace Maternity &Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
authors.affiliation: International Peace Maternity &Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: International Peace Maternity &Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Lin
authors.affiliation: International Peace Maternity &Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Yulei
authors.initials: Y
authors.lastname: Li
authors.affiliation: International Peace Maternity &Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Hefeng
authors.initials: H
authors.lastname: Huang
authors.affiliation: International Peace Maternity &Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism; D000818:Animals; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D013087:Spermatids / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D062085:RNA, Long Noncoding; D012333:RNA, Messenger
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