title: Assessing the role of selected growth factors and cytostatic agents in an in vitro model of human dura mater healing.
abstract: OBJECTIVE
  Cerebrospinal fluid (CSF) leaks are a common concern in skull base surgery. Appropriate dural healing is crucial to prevent CSF leaks but the entire process has been barely understood so far. Here, we review the impact of growth factors and chemotherapeutic agents on an explant culture of human dural fibroblasts and a 3D subculture grown in a collagen mesh scaffold.
  
  
  METHODS
  Human dural specimens were harvested during surgical procedures where they would not be further used therapeutically or diagnostically. Explant cultures were grown in Petri dishes, and subcultures were grown in collagen mesh scaffolds. Insulin, fibroblast growth factor type 2 (FGF-2), and human serum were analyzed for their effect as growth factors, whereas mitomycin C, vincristine, and colchicine were analyzed for their role as inhibitors. Cell count was used as a parameter to assess the effects of these factors. In addition, the effects of human serum were assessed using collagen mesh scaffolds.
  
  
  RESULTS
  Insulin, FGF-2, and human serum increased culture cell count; human serum also achieved an increased number of viable fibroblasts embedded in a collagen mesh. Mitomycin C, which is a mitosis inhibitor, showed no significant effect on cell count, whereas colchicine and vincristine, which inhibit both mitosis and migration, resulted in cell growth suppression.
  
  
  CONCLUSIONS
  In our model, dural defect closure is achieved through cell migration rather than through cell growth. Adding growth factors to the dural suture line or into a collagen mesh might prove useful to stimulate dural closure.
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Goldschmidt
authors.forename: Marcelo
authors.initials: M
authors.lastname: Ielpi
authors.forename: Monica
authors.initials: M
authors.lastname: Loresi
authors.forename: Maximiliano
authors.initials: M
authors.lastname: D'adamo
authors.forename: Diego
authors.initials: D
authors.lastname: Giunta
authors.forename: Antonio
authors.initials: A
authors.lastname: Carrizo
authors.forename: Pablo
authors.initials: P
authors.lastname: Ajler
authors.forename: Claudio
authors.initials: C
authors.lastname: Yampolsky
authors.forename: Pablo
authors.initials: P
authors.lastname: Argibay
mesh_terms: D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D054697:Cytostatic Agents / Q000494:pharmacology*; D004388:Dura Mater / Q000187:drug effects* / Q000503:physiopathology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D044967:Serum; D014945:Wound Healing / Q000187:drug effects
chemical_list: D054697:Cytostatic Agents; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D016222:Fibroblast Growth Factor 2; D003094:Collagen
keywords: CSF leaks,; Chemotherapy,; Dura mater,; Growth factors