title: PU.1 promotes miR-191 to inhibit adipogenesis in 3T3-L1 preadipocytes.
abstract: PU.1, an Ets family transcription factor, was previously demonstrated expressed in 3T3-L1 preadipocytes and had an negative effect on adiopogenesis. However, the underlying mechanism remains elusive. Here, miR-191 was identified as an inhibitor of adipocyte differentiation through targeting the 3' untranslated regions of C/EBPβ, the initial factor in the C/EBPα/β-PPARγ terminal pathway of adipogenic differentiation. MiR-191 suppressed the lipid accumulation by Oil Red O staining and downregulated the levels of adipogenic marker genes PPARγ (P<0.01), aP2 (P<0.01) and FAS (P<0.05). Then, we found that PU.1 overexpression resulted in upregulation of miR-191 and adipogenic inhibition. Likewise, PU.1 overexpression rescued the miR-191 decrease and resisted the adipogenic promotion caused by miR-191 oligonucleotide inhibitor. Collectively, these results revealed that PU.1 promoted miR-191 to suppress adipogenesis 3T3-L1 preadipocyte and indicated a new mechanism of PU.1 inhibiting adipogenesis.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.
authors.forename: Shengyue
authors.initials: S
authors.lastname: Ji
authors.affiliation: Beijing Shuita Aged-vinegar Research Institute for Biosciences, Beijing 102200, PR China.
authors.forename: Weili
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Bao
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Han
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China.
authors.forename: Fanxu
authors.initials: F
authors.lastname: Meng
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, PR China. Electronic address: jiziqing2010@163.com.
authors.forename: Binyun
authors.initials: B
authors.lastname: Cao
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics* / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D017930:Genes, Reporter; D049410:Luciferases, Renilla / Q000235:genetics; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics*; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D020413:3' Untranslated Regions; D022782:CCAAT-Enhancer-Binding Protein-beta; D049410:Luciferases, Renilla; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C545013:Cebpb protein, mouse; C555937:MIRN191 microRNA, mouse
keywords: 3T3-L1 preadipocytes; Adipogenesis; C/EBPβ; PU.1; miR-191