title: Folate receptor targeted self-assembled chitosan-based nanoparticles for SPECT/CT imaging: demonstrating a preclinical proof of concept.
abstract: A new biocompatible, biodegradable, self-assembled chitosan-based nanoparticulate product was successfully synthesized and radiolabeled with technetium-99m, and studied as a potential new SPECT or SPECT/CT imaging agent for diagnosis of folate receptor overexpressing tumors. In the present study we examined the conditions of a preclinical application of this labeled nanosystem in early diagnosis of spontaneously diseased veterinary patient using a human SPECT/CT device. The results confirmed that the nanoparticles accumulated in tumor cells overexpressing folate receptors, contrast agent revealed higher uptake in the tumor for a long time. Preclinical trials verified that the new nanoparticles are able to detect folate-receptor-overexpressing tumors in spontaneously diseased animal models with enhanced contrast.
authors.affiliation: National "F.J.C." Research Institute for Radiobiology and Radiohygiene (NRIRR), Budapest, Hungary. Electronic address: bandi.polyak@gmail.com.
authors.forename: András
authors.initials: A
authors.lastname: Polyák
authors.affiliation: BBS Nanotechnology Ltd., Debrecen, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Hajdu
authors.affiliation: BBS Nanotechnology Ltd., Debrecen, Hungary.
authors.forename: Magdolna
authors.initials: M
authors.lastname: Bodnár
authors.affiliation: Department of Nuclear Medicine, Semmelweis University, Budapest, Hungary.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Dabasi
authors.affiliation: Department of Nuclear Medicine, Semmelweis University, Budapest, Hungary.
authors.forename: Róbert P
authors.initials: RP
authors.lastname: Jóba
authors.affiliation: BBS Nanotechnology Ltd., Debrecen, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Borbély
authors.affiliation: National "F.J.C." Research Institute for Radiobiology and Radiohygiene (NRIRR), Budapest, Hungary.
authors.forename: Lajos
authors.initials: L
authors.lastname: Balogh
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D002415:Cats; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry*; D005260:Female; D058973:Folate Receptors, GPI-Anchored / Q000032:analysis* / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000378:metabolism; D064847:Multimodal Imaging / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D058973:Folate Receptors, GPI-Anchored; D019275:Radiopharmaceuticals; D048271:Chitosan
keywords: (99m)Tc; Chitosan; Folate receptor; Nanomedicine; Nanoparticles; SPECT-CT