title: Rapid screening of low-molecular-weight phenols from persimmon (Diospyros kaki) pulp using liquid chromatography/UV-visible/electrospray mass spectrometry analysis.
abstract: BACKGROUND
  Persimmon fruits have been widely used in traditional medicine owing to their phenolic composition. This research aims to perform a rapid, detailed and affordable study of the profile of low-molecular-weight phenols from persimmon pulp.
  
  
  RESULTS
  Two different HPLC-DAD/ESI-MS(n) analyses were performed using a routine three-dimensional ion trap mass spectrometer to analyze the ethanolic extract of persimmon pulp: (1) an untargeted data-dependent analysis to identify the majority of small phenols that included full MS and MS(2) scan events; (2) a targeted data-dependent analysis to identify polymerized phenols (dimers and formic acid adducts) through a source-induced dissociation analysis that included full MS and MS(2) scan events. Thirty-two low-molecular-weight phenols were detected, comprising gallic acid and its glycoside and acyl derivatives, glycosides of p-coumaric, vanillic and cinnamic acids and different flavone di-C-hexosides, most of them reported for the first time in persimmon.
  
  
  CONCLUSIONS
  The use of a straightforward and affordable methodology of analysis led to obtain an up-to-date profiling of low-molecular-weight phenols in persimmon. The results can help future actions aimed to expand the understanding of the phenolic metabolome of persimmon cultivars.
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Avda Agustín Escardino 7, E-46980, Paterna, Valencia, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Sentandreu
authors.forename: Manuela
authors.initials: M
authors.lastname: Cerdán-Calero
authors.forename: John M
authors.initials: JM
authors.lastname: Halket
authors.forename: José L
authors.initials: JL
authors.lastname: Navarro
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029788:Diospyros / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000032:analysis; D008970:Molecular Weight; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010636:Phenols; D010936:Plant Extracts; D005707:Gallic Acid
keywords: Diospyros kaki; HPLC-DAD/ESI-MSn analysis; low-molecular-weight phenols; persimmon pulp; routine three-dimensional ion trap