title: SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins.
abstract: The aim of this study was to explore the role of SecDF in protein secretion in Bacillus cereus ATCC 14579 by in-depth characterization of a markerless secDF knock out mutant. Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. Most toxins, including food poisoning-associated enterotoxins Nhe, Hbl, and cytotoxin K, as well as phospholipase C were less abundant in the secretome of the ΔsecDF mutant as determined by label-free mass spectrometry. Global transcriptome studies revealed profound transcriptional changes upon deletion of secDF indicating cell envelope stress. Interestingly, the addition of glucose enhanced the described phenotypes. This study shows that SecDF is an important part of the Sec-translocase mediating efficient secretion of virulence factors in the Gram-positive opportunistic pathogen B. cereus, and further supports the notion that B. cereus enterotoxins are secreted by the Sec-system.
authors.affiliation: Laboratory for Microbial Dynamics (LaMDa), Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.
authors.forename: Aniko
authors.initials: A
authors.lastname: Vörös
authors.affiliation: Laboratory for Microbial Dynamics (LaMDa), Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.
authors.forename: Roger
authors.initials: R
authors.lastname: Simm
authors.affiliation: INRA, UMR1319 Micalis, Domaine de La Minière, Guyancourt, France.
authors.forename: Leyla
authors.initials: L
authors.lastname: Slamti
authors.affiliation: Australian Proteome Analysis Facility (APAF), Macquarie University, Sydney, Australia.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: McKay
authors.affiliation: Laboratory for Microbial Dynamics (LaMDa), Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.
authors.forename: Ida K
authors.initials: IK
authors.lastname: Hegna
authors.affiliation: INRA, UMR1319 Micalis, Domaine de La Minière, Guyancourt, France.
authors.forename: Christina
authors.initials: C
authors.lastname: Nielsen-LeRoux
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.
authors.forename: Karl A
authors.initials: KA
authors.lastname: Hassan
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.
authors.forename: Ian T
authors.initials: IT
authors.lastname: Paulsen
authors.affiliation: INRA, UMR1319 Micalis, Domaine de La Minière, Guyancourt, France; AgroParistech, UMR Micalis, Jouy-en-Josas, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Lereclus
authors.affiliation: Laboratory for Microbial Dynamics (LaMDa), Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.
authors.forename: Ole Andreas
authors.initials: OA
authors.lastname: Økstad
authors.affiliation: Australian Proteome Analysis Facility (APAF), Macquarie University, Sydney, Australia.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Molloy
authors.affiliation: Laboratory for Microbial Dynamics (LaMDa), Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.
authors.forename: Anne-Brit
authors.initials: AB
authors.lastname: Kolstø
mesh_terms: D000818:Animals; D001409:Bacillus cereus / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001692:Biological Transport; D002473:Cell Wall / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D009036:Moths / Q000382:microbiology; D009154:Mutation; D013312:Stress, Physiological; D014774:Virulence / Q000235:genetics; D015262:Xenobiotics / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D015262:Xenobiotics
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