title: Expression pattern and tissue localization of the class B scavenger receptor BmSCRBQ4 in Bombyx mori.
abstract: Class B scavenger receptors (SR-Bs) are cell surface glycoproteins involved in various physiological processes in vivo, including the transport and metabolism of lipids, binding and phagocytosis of xenobiotics, and signaling. But little information is available about silkworm SR-Bs; it is necessary to study these SR-Bs for revealing their function. In this study, we cloned the full-length coding sequence of BmSCRBQ4, a SR-B gene from the silkworm Bombyx mori L. We found that the BmSCRBQ4 gene consists of nine exons and eight introns, with an open reading frame of 1371 bp encoding 456 amino acids. Gene expression studies determined that BmSCRBQ4 messenger RNA (mRNA) was expressed in unfertilized eggs, during embryonic development and throughout the majority of the larval period. Expression of mRNA was detected in the mid gut, middle silk gland, posterior silk gland, head, integumentum, fat body, testes and the ovaries of the larval B. mori Dazao strain, as well as in the silkworm cell lines BmN and BmE. Protein expression studies found BmSCRBQ4 protein was expressed only in the testes, fat body and middle silk gland of larvae, as well as in the silkworm cell lines BmN and BmE. The BmSCRBQ4 protein showed variability in banding patterns in different tissues and cells when analyzed by Western blotting. Immunohistochemical staining showed that the BmSCRBQ4 protein localizes to the constitutive membranes or cellular membranes of these tissues. These results indicated that BmSCRBQ4 gene may play some physiologically relevant roles at the cell surface in each tissue.
authors.affiliation: Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan.
authors.forename: Zhan-Peng
authors.initials: ZP
authors.lastname: Dong
authors.affiliation: College of Biotechnology, Southwest University, Chongqing.
authors.forename: Chun-Li
authors.initials: CL
authors.lastname: Chai
authors.affiliation: Institute of Sericulture and System Biology, Southwest University, Chongqing.|College of Biotechnology, Southwest University, Chongqing.
authors.forename: Fang-Yin
authors.initials: FY
authors.lastname: Dai
authors.affiliation: Institute of Sericulture and System Biology, Southwest University, Chongqing.|College of Biotechnology, Southwest University, Chongqing.
authors.forename: Min-Hui
authors.initials: MH
authors.lastname: Pan
authors.affiliation: Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan.
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Southwest University, Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Liao
authors.affiliation: Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Sericulture and System Biology, Southwest University, Chongqing.|College of Biotechnology, Southwest University, Chongqing.|College of Animal Science and Technology, Southwest University, Chongqing, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D008969:Molecular Sequence Data; D051122:Scavenger Receptors, Class B / Q000235:genetics / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D051122:Scavenger Receptors, Class B
keywords: BmSCRBQ4; Bombyx mori; Cameo2; class B scavenger receptor; gene expression; immunohistochemistry