title: Transcription. Comment on "Mechanism of eukaryotic RNA polymerase III transcription termination".
abstract: Nielsen et al. (Reports, 28 June 2013, p. 1577) characterized their RNA polymerase III (Pol III) preparation and concluded that it requires an RNA hairpin/duplex structure for terminating transcription. We could not corroborate their findings using bona fide Pol III from two laboratory sources. We show that Pol III efficiently terminates transcription in the absence of a hairpin/duplex in vitro and in vivo.
authors.affiliation: Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Aneeshkumar G
authors.initials: AG
authors.lastname: Arimbasseri
authors.affiliation: Division of Biological Sciences, University of California, San Diego, CA, USA.
authors.forename: George A
authors.initials: GA
authors.lastname: Kassavetis
authors.affiliation: Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA. Commissioned Corps, U.S. Public Health Service. maraiar@mail.nih.gov.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Maraia
mesh_terms: D012320:RNA Polymerase III / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D061806:Transcription Termination, Genetic
chemical_list: D012320:RNA Polymerase III
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