title: The Tibetan herbal medicines Padma 28 and Padma Circosan inhibit the formation of advanced glycation endproducts (AGE) and advanced oxidation protein products (AOPP) in vitro.
abstract: BACKGROUND
  Advanced glycation endproducts (AGE) and advanced oxidation protein products (AOPP) play a pivotal role in the development of diabetes associated diseases. The herbal medicines Padma 28 and Padma Circosan have shown effectiveness in symptoms of diabetes associated diseases and have antioxidant effects. It is not known whether inhibition of AGE and AOPP formation is a mechanism of their action.
  
  
  METHODS
  BSA was subjected to glycation or oxidation with or without 70% ethanolic extracts of Padma 28, Padma Circosan or with an active control. AGE and AOPP concentrations were analyzed fluorimetrically or spectrophotometrically respectively and by ELISA.
  
  
  RESULTS
  Compared to the positive control Padma 28, Padma Circosan and the active control significantly reduced AGE levels by 58.6%, 56.7%, and 8.14% (fluorimetry) and by 35.48, 34.19, and 19.68% (ELISA). AOPP were reduced by 57.28/66.78% (30'/60' incubation), by 67.08/71.99%, and by 81.68/86.54% (spectrophotometry) or by 79.98/86.97%, 79.3/84.3% and 77.07/90.31% (ELISA). All results are significantly different (p < 0.001). No difference was found between the effects of the two preparations.
  
  
  CONCLUSIONS
  Both formulas significantly inhibited the formation of AGE and AOPP to a similar extent as the active controls. This suggests a possible role for both Padma preparations in the treatment and prevention of diabetes associated diseases.
authors.affiliation: Department of Pharmaceutical Biochemistry, Wroclaw Medical University, Wroclaw, Poland. ewa.grzebyk@gmail.com.
authors.forename: Ewa
authors.initials: E
authors.lastname: Grzebyk
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Piwowar
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D062107:Advanced Oxidation Protein Products; D017127:Glycation End Products, Advanced; D010936:Plant Extracts; C066069:Padma 28; D012710:Serum Albumin, Bovine
references.citation: Diabetes Care. 2013 Jan;36(1):11-2
references.pmid: 23264287
references.citation: Diabetes Metab Res Rev. 2001 May-Jun;17(3):189-212
references.pmid: 11424232
references.citation: Phytother Res. 2008 Feb;22(2):228-37
references.pmid: 17886226
references.citation: Pol Arch Med Wewn. 2013;123(7-8):364-70
references.pmid: 23648800
references.citation: Ann Nutr Metab. 2010;57(1):40-9
references.pmid: 20668372
references.citation: Inflammopharmacology. 1999;7(1):47-62
references.pmid: 17657446
references.citation: Zhong Yao Cai. 2010 Jan;33(1):73-6
references.pmid: 20518309
references.citation: Fitoterapia. 2005 Jul;76(5):419-27
references.pmid: 15894431
references.citation: Mutat Res. 2005 Nov 11;579(1-2):37-46
references.pmid: 16084533
references.citation: BMJ. 2011 Jul 26;343:d4169
references.pmid: 21791495
references.citation: Clin Cardiol. 2014 Sep;37(9):576-81
references.pmid: 24863141
references.citation: Redox Rep. 2000;5(1):17-22
references.pmid: 10905539
references.citation: Eur J Clin Chem Clin Biochem. 1997 Sep;35(9):669-77
references.pmid: 9352229
references.citation: Forsch Komplementmed. 2013;20 Suppl 2:33-4
references.pmid: 23860112
references.citation: Forsch Komplementmed. 2013;20 Suppl 2:25-30
references.pmid: 23860110
references.citation: Phytother Res. 2011 May;25(5):740-3
references.pmid: 21413090
references.citation: Zhongguo Zhong Yao Za Zhi. 2008 Jun;33(11):1313-6
references.pmid: 18831216
references.citation: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2005 Jun;149(1):83-7
references.pmid: 16170393
references.citation: Lancet. 2011 Jul 2;378(9785):31-40
references.pmid: 21705069
references.citation: Am J Chin Med. 2011;39(4):827-38
references.pmid: 21721160
references.citation: Forsch Komplementmed. 2006 Feb;13 Suppl 1:1-6
references.pmid: 16582556
references.citation: Circ Res. 2009 Mar 27;104(6):750-7
references.pmid: 19179658
references.citation: Vasa. 2005 Feb;34(1):11-7
references.pmid: 15786932
references.citation: Kidney Int. 1996 May;49(5):1304-13
references.pmid: 8731095