title: A germline mutation in the miR‑125a coding region reduces miR‑125a expression and is associated with human gastric cancer.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that inhibit the expression of target protein-coding genes, most often at the post-transcriptional level. miRNAs are often found to be misregulated in human cancer and they can act as potent oncogenes or tumor suppressor genes. In this study, we found that a germline mutation in the miR-125a coding region is associated with human gastric cancer. This mutation reduced the expression of mature miR-125a and alleviated its inhibitory effect on erythroblastic leukemia viral oncogene homolog 2 (ERBB2) gene expression and on gastric tumor cell proliferation. Thus, the data of this study suggested that this germline mutation in pri‑miR-125a likely contributes to the genetic predisposition to gastric cancer by reducing the production of miR-125a, thereby interfering with the expression of miR-125a target genes.
authors.affiliation: Department of Gastroenterology, Qilu Hospital of Shangdong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Shang
authors.affiliation: Department of Gastrointestinal Surgery, Linzi District People's Hospital 255400, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: The 94th Hospital of Chinese People's Liberation Army, Nanchang, Jiangxi 330002, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Shang
authors.affiliation: Department of Gastroenterology, General Hospital of Zibo, Zibo, Shandong 255036, P.R. China.
authors.forename: Kun-Ming
authors.initials: KM
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology, Qilu Hospital of Shangdong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Li
mesh_terms: D000483:Alleles; D045744:Cell Line, Tumor; D005753:Gastric Mucosa / Q000378:metabolism; D005787:Gene Frequency; D005838:Genotype; D018095:Germ-Line Mutation; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D035683:MicroRNAs; D018719:Erb-b2 Receptor Tyrosine Kinases; C531888:MIRN125 microRNA, human; C508053:ERBB2 protein, human