title: Response of plasma membrane H+-ATPase in rice (Oryza sativa) seedlings to simulated acid rain.
abstract: Understanding the adaptation of plants to acid rain is important to find feasible approaches to alleviate such damage to plants. We studied effects of acid rain on plasma membrane H(+)-ATPase activity and transcription, intracellular H(+), membrane permeability, photosynthetic efficiency, and relative growth rate during stress and recovery periods. Simulated acid rain at pH 5.5 did not affect plasma membrane H(+)-ATPase activity, intracellular H(+), membrane permeability, photosynthetic efficiency, and relative growth rate. Plasma membrane H(+)-ATPase activity and transcription in leaves treated with acid rain at pH 3.5 was increased to maintain ion homeostasis by transporting excessive H(+) out of cells. Then intracellular H(+) was close to the control after a 5-day recovery, alleviating damage on membrane and sustaining photosynthetic efficiency and growth. Simulated acid rain at pH 2.5 inhibited plasma membrane H(+)-ATPase activity by decreasing the expression of H(+)-ATPase at transcription level, resulting in membrane damage and abnormal intracellular H(+), and reduction in photosynthetic efficiency and relative growth rate. After a 5-day recovery, all parameters in leaves treated with pH 2.5 acid rain show alleviated damage, implying that the increased plasma membrane H(+)-ATPase activity and its high expression were involved in repairing process in acid rain-stressed plants. Our study suggests that plasma membrane H(+)-ATPase can play a role in adaptation to acid rain for rice seedlings.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China, liangchanjuan@jiangnan.edu.cn.
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Liang
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Ge
authors.forename: Lei
authors.initials: L
authors.lastname: Su
authors.forename: Jinjin
authors.initials: J
authors.lastname: Bu
mesh_terms: D015258:Acid Rain / Q000633:toxicity*; D000222:Adaptation, Physiological; D000251:Adenosine Triphosphatases; D001692:Biological Transport; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D015870:Gene Expression / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012275:Oryza / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology; D010944:Plants / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000187:drug effects*; D036226:Seedlings / Q000187:drug effects* / Q000201:enzymology; D013312:Stress, Physiological
chemical_list: D015258:Acid Rain; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases
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