title: Effective stimulation of the biotechnological potential of the medicinal white rot fungus: Phellinus pini by menadione-mediated oxidative stress.
abstract: The effect of menadione (MQ; 2-methyl-1,4-naphtoquinone), a superoxide-generating agent, on the natural biodegradation system in the medicinal white rot fungus Phellinus pini was determined. While measuring the activities of extracellular manganese-dependent peroxidase (MnP) and intracellular chitinase, it was found that the application of MQ (0.75 mM) distinctly stimulated the activities of these enzymes in comparison to the control values (without MQ). Using the capillary electrophoresis (CE) method, an increase in the extracellular oxalic acid (OXA) concentration was detected during the first days after the addition of MQ. It was observed that the rate of intracellular proteolysis at pH 3.5 evidently decreased under oxidative stress conditions. Contrary to these results, the activities of serine proteases at pH 9.5 measured against fluorogenic peptide substrates distinctly increased in stressed cultures. The MQ treatment also caused an evident increase in the catalase (CAT) activity, as well as the levels of superoxide anion radicals (SORs), formaldehyde (FA), and phenolic compounds (PHC) in the experimental cultures. The results obtained confirm that prooxidants may find application as an effective way to stimulate biotechnological production of MnP and chitinase by white rot fungi.
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, 19 Akademicka Street, 20-033, Lublin, Poland, magdalena.jaszek@poczta.umcs.lublin.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Jaszek
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kos
authors.forename: Anna
authors.initials: A
authors.lastname: Matuszewska
authors.forename: Marcin
authors.initials: M
authors.lastname: Grąz
authors.forename: Dawid
authors.initials: D
authors.lastname: Stefaniuk
authors.forename: Monika
authors.initials: M
authors.lastname: Osińska-Jaroszuk
authors.forename: Monika
authors.initials: M
authors.lastname: Prendecka
authors.forename: Ewa
authors.initials: E
authors.lastname: Jóźwik
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Grzywnowicz
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D001709:Biotechnology; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D018384:Oxidative Stress / Q000187:drug effects*; D059748:Proteolysis; D024483:Vitamin K 3 / Q000494:pharmacology*
chemical_list: D024483:Vitamin K 3
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