title: Features of membrane receptors in bacterial multiplication process and necessary conditions for phage infection of bacteria.
abstract: According to the obtained experimental results, the thermal shock (from 37 to 53 °C) not only stops the multiplication process of Escherichia coli bacteria, but also causes bacterial titer to decrease gradually. After this period lasting up to 1 hour, the bacterial cells continue to grow. A similar type of response was observed when bacteria were subjected to acid shock. Increasing acidity of media leads to decrease of bacterial growth process, and finally, their titer curve sharply falls over time. Also, interesting results were obtained about necessary conditions for infecting the bacteria by phages. Particularly, DNA injection from phages into bacterial cells requires most of corresponding bacterial membrane receptors to be occupied by phages. We suppose that this occurs due to autocrine phenomenon when the signaling molecules block the DNA ejection from phage particles. This effect lasts until a certain number of phage particles are attached to the membrane. After that, DNA injection from phage head into the cytoplasm takes place and the process of bacterial infection begins. The real number of phages in a stock is by several orders higher than the number of plaque-forming units in a given stock, which is determined by a classical double-layer agar method.
authors.affiliation: Faculty of Exact and Natural Sciences, I.Javakhishvili Tbilisi State University, 3, Chavchavadze ave., 0179, Tbilisi, Georgia, tamaz.mdzinarashvili@tsu.ge.
authors.forename: Tamaz
authors.initials: T
authors.lastname: Mdzinarashvili
authors.forename: Irina
authors.initials: I
authors.lastname: Papukashvili
authors.forename: Nino
authors.initials: N
authors.lastname: Shengelia
authors.forename: Mariam
authors.initials: M
authors.lastname: Khvedelidze
mesh_terms: D003090:Coliphages / Q000254:growth & development / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects / Q000821:virology*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D010948:Viral Plaque Assay; D053585:Virus Attachment; D053586:Virus Internalization
chemical_list: D003470:Culture Media
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