title: A novel angiotensin-І converting enzyme (ACE) inhibitory peptide from gastrointestinal protease hydrolysate of silkworm pupa (Bombyx mori) protein: Biochemical characterization and molecular docking study.
abstract: Silkworm pupa (Bombyx mori) protein was hydrolyzed using gastrointestinal endopeptidases (pepsin, trypsin and α-chymotrypsin). Then, the hydrolysate was purified sequentially by ultrafiltration, gel filtration chromatography and RP-HPLC. A novel ACE inhibitory peptide, Ala-Ser-Leu, with the IC50 value of 102.15μM, was identified by IT-MS/MS. This is the first report of Ala-Ser-Leu from natural protein. Lineweaver-Burk plots suggest that the peptide is a competitive inhibitor against ACE. The molecular docking studies revealed that the ACE inhibition of Ala-Ser-Leu is mainly attributed to forming very strong hydrogen bonds with the S1 pocket (Ala354) and the S2 pocket (Gln281 and His353). The results indicate that silkworm pupa (B. mori) protein or its gastrointestinal protease hydrolysate could be used as a functional ingredient in auxiliary therapeutic foods against hypertension.
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu Province 212018, PR China. Electronic address: wuqy1@163.com.
authors.forename: Qiongying
authors.initials: Q
authors.lastname: Wu
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu Province 212018, PR China. Electronic address: jiajq@just.edu.cn.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Jia
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu Province 212018, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province 212018, PR China.
authors.forename: Jinjuan
authors.initials: J
authors.lastname: Du
authors.affiliation: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu Province 212018, PR China.
authors.forename: Zhongzheng
authors.initials: Z
authors.lastname: Gui
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D020134:Catalytic Domain; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D041981:Gastrointestinal Tract / Q000201:enzymology; D006801:Humans; D006868:Hydrolysis; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification; D062105:Molecular Docking Simulation; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000737:chemistry; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry; D011485:Protein Binding; D059748:Proteolysis
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D019476:Insect Proteins; D010447:Peptide Hydrolases; D007703:Peptidyl-Dipeptidase A
keywords: ACE inhibitory peptide; Gastrointestinal protease; Inhibition pattern; Molecular docking; Silkworm pupa (Bombyx mori)