title: Identification of velvet antler by random amplified polymorphism DNA combined with non-gel sieving capillary electrophoresis.
abstract: Mitochondrial DNA of velvet antler was amplified with random amplified polymorphic DNA (RAPD) technique and the PCR products were detected with non-gel sieving capillary electrophoresis to establish a RAPD-HPCE method used for identifying the authenticity of velvet antler or it counterfeits. Factors that could affect the PCR amplification and capillary electrophoresis were optimized. Under the optimized conditions, namely, 20 mmol L(-1) NaH2PO4-Na2HPO4-2 mmol L(-1) EDTA buffer solution [0.8% (W/V) HPMC, 15 mmol L(-1) TBAP and pH 7.3], -10 kV injection voltage and -8 kV separation voltage, Cervus nippon Temminck antler, Cervus elaphus Linnaeus antler, Rangifer tarandus antler, Cervus canadensis antler and Elaphurus davidianus antler were analyzed. The analysis on the similarity of obtained elctrophoretograms showed that there were significant differences in similarities of different velvet antlers, which could be used for the quick identification of the authenticity of velvet antler samples. It can be found that the technique of RAPD combined with HPCE is advantageous in rich polymorphism, high detection rate, simple and convenient performance, high efficiency, rapidness and sensitivity, indicating that it should be suitable for the quick identification of the authenticity of velvet antler samples.
authors.affiliation: a College of Pharmacy, Beihua University , Jilin , PR China .|b Biological Macromolecular Function Developments and Application of Engineering Laboratory , Jilin , PR China .
authors.forename: Guangxin
authors.initials: G
authors.lastname: Yuan
authors.affiliation: a College of Pharmacy, Beihua University , Jilin , PR China .
authors.forename: Jiyan
authors.initials: J
authors.lastname: Sun
authors.affiliation: a College of Pharmacy, Beihua University , Jilin , PR China .
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.affiliation: c School of Laboratory Medicine, Beihua University , Jilin , PR China , and.
authors.forename: Guilian
authors.initials: G
authors.lastname: Fu
authors.affiliation: a College of Pharmacy, Beihua University , Jilin , PR China .
authors.forename: Guangyu
authors.initials: G
authors.lastname: Xu
authors.affiliation: c School of Laboratory Medicine, Beihua University , Jilin , PR China , and.
authors.forename: Mingcheng
authors.initials: M
authors.lastname: Li
authors.affiliation: a College of Pharmacy, Beihua University , Jilin , PR China .|d LeiBo Technology Co., Ltd. , Jilin , PR China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a College of Pharmacy, Beihua University , Jilin , PR China .
authors.forename: Xintian
authors.initials: X
authors.lastname: Fan
mesh_terms: D000818:Animals; D000999:Antlers; D053801:Capillary Electrochromatography; D004272:DNA, Mitochondrial / Q000235:genetics*; D003670:Deer / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
chemical_list: D004272:DNA, Mitochondrial
keywords: Identification; non-gel sieving capillary electrophoresis (HPCE); random amplified polymorphic DNA (RAPD); velvet antler