title: Surface modification of HSA containing magnetic PLGA nanoparticles by poloxamer to decrease plasma protein adsorption.
abstract: Lifetime prolongation for hydrophobic drug carriers has been the focus of interest for many years. Poloxamer (Pluronic F68, PF68) has been employed in this study for modifying the surface of magnetic poly(d,l-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with human serum albumin (HSA) model drug. Surface characteristics of untreated and PF68 treated NPs were analyzed by size, zeta potential and electrophoretic mobility studies. UV-vis spectroscopic analysis, isothermal titration calorimetry (ITC) and dynamic light scattering methods were used to investigate serum protein (bovine serum albumin, BSA) adsorption. Results showed the successful surface attachment of PF68. Among different concentrations (0.1-1%, wt/vol) of PF68 studied, 0.5% was found to be the most useful, since a higher concentration can issue in micelle formation. 50% less BSA tended to be adsorbed on the treated NPs in comparison to the untreated ones.
authors.affiliation: Doctoral School of Molecular and Nanotechnologies, Faculty of Information Technology, University of Pannonia, Egyetem u.10, H-8200 Veszprém, Hungary; Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u.10, H-8200 Veszprém, Hungary. Electronic address: shubhro.du@gmail.com.
authors.forename: Quazi T H
authors.initials: QTH
authors.lastname: Shubhra
authors.affiliation: Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u.10, H-8200 Veszprém, Hungary; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok körútja 2, H-1117 Budapest, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Tóth
authors.affiliation: Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u.10, H-8200 Veszprém, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Gyenis
authors.affiliation: Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u.10, H-8200 Veszprém, Hungary; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok körútja 2, H-1117 Budapest, Hungary.
authors.forename: Tivadar
authors.initials: T
authors.lastname: Feczkó
mesh_terms: D000327:Adsorption; D002151:Calorimetry; D024202:Electrophoretic Mobility Shift Assay; D019344:Lactic Acid / Q000737:chemistry*; D008280:Magnetics; D053758:Nanoparticles; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012709:Serum Albumin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties
chemical_list: D012709:Serum Albumin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Isothermal titration calorimetry; Poloxamer; Protein adsorption; Surface modification