title: Efficacy of plant-derived compounds combined with hydrogen peroxide as antimicrobial wash and coating treatment for reducing Listeria monocytogenes on cantaloupes.
abstract: The efficacy of four plant-derived antimicrobials (PDAs), namely carvacrol, thymol, β-resorcylic acid, and caprylic acid, with or without hydrogen peroxide (HP), as antimicrobial wash and chitosan based coating for reducing Listeria monocytogenes (LM) on cantaloupes was investigated. Cantaloupe rind plugs inoculated with LM (10(7) CFU/cm(2)) were washed for 3, 6, 10 min at 25 °C or 1, 3, 5 min at 55 or 65 °C in water, or water containing 2% PDAs with or without 2% HP. Additionally, inoculated cantaloupes (10(8) CFU/fruit) washed with 2% PDA-HP combinations at 55 or 65 °C (5 min) were cut into rindless cubical pieces, stored at 4 °C for 7 days and sampled for LM. Furthermore, inoculated plugs coated with 2% PDAs were stored for 7 days and sampled for surviving LM. Individual PDA washes reduced LM on rinds by ≥2.5 log CFU/cm(2) by 3 min (P < 0.05). PDA-HP combinations decreased LM to undetectable levels by 5 min at 55, 65 °C, and 10 min at 25 °C (P < 0.05) and reduced LM transfer from cantaloupe surface to interior (P < 0.0001). All PDA coating treatments reduced LM on cantaloupe to undetectable levels by 5 days (P < 0.05). Results indicate that PDAs alone, or with HP could be used to reduce LM on cantaloupes.
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Abhinav
authors.initials: A
authors.lastname: Upadhyay
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Indu
authors.initials: I
authors.lastname: Upadhyaya
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Shankumar
authors.initials: S
authors.lastname: Mooyottu
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Anup
authors.initials: A
authors.lastname: Kollanoor-Johny
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA. Electronic address: kumar.venkitanarayanan@uconn.edu.
authors.forename: Kumar
authors.initials: K
authors.lastname: Venkitanarayanan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry; D028463:Cucumis melo / Q000737:chemistry / Q000382:microbiology*; D004202:Disinfectants / Q000494:pharmacology*; D005511:Food Handling / Q000379:methods*; D005519:Food Preservation / Q000379:methods*; D005638:Fruit / Q000737:chemistry / Q000382:microbiology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004202:Disinfectants; D010936:Plant Extracts; D048271:Chitosan; D006861:Hydrogen Peroxide
keywords: Cantaloupes; Chitosan; Hydrogen peroxide; Inactivation; Listeria monocytogenes; Plant-derived antimicrobials