title: Enhanced osteogenesis of bone morphology protein-2 in 2-N,6-O-sulfated chitosan immobilized PLGA scaffolds.
abstract: By using 2-N,6-O-sulfated chitosan (26SCS) immobilized poly(lactide-co-glycolide) (PLGA) scaffolds, our system achieved controlled release and improved bioactivity of recombinant human bone morphology protein-2 (rhBMP-2). Initially aminolyzed by ethylenediamine, PLGA scaffolds surface was immobilized with 26SCS via electrostatic assembly. Upon the presence of 26SCS, the system displayed improved rhBMP-2 adsorption and prolonged release process in vitro due to the high affinity of rhBMP-2 with 26SCS. On the other hand, because of incorporation of 26SCS, the system appeared to be more hydrophilic and provided a better environment for cells attachment. Moreover, 26SCS enhanced the binding efficiency between rhBMP-2 and its receptors as well as alkaline phosphatase activity. Our study highlights 26SCS immobilized PLGA scaffolds may be excellent candidates for use in bone tissue engineering.
authors.affiliation: Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Kong
authors.affiliation: Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: biomatwj@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Lingyan
authors.initials: L
authors.lastname: Cao
authors.affiliation: Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Yuanman
authors.initials: Y
authors.lastname: Yu
authors.affiliation: The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China; Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Changsheng
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D056951:Photoelectron Spectroscopy; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011994:Recombinant Proteins / Q000737:chemistry; D055672:Static Electricity
chemical_list: D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan
keywords: Bone morphology protein-2; Osteogenesis; Scaffold; Sulfated chitosan; Surface modification