title: Shortening the culture time in cytogenetic dosimetry using PCC-R assay.
abstract: The fast assessment of the dose received by exposed persons is crucial in radiological accidents, so the 48 h of cell culture in conventional cytogenetic dosimetry in addition to some limitations after high doses becomes a disadvantage. The premature chromosome condensation (PCC) assay permits to analyse enough cells after high radiation exposure, and the score of PCC-R may reduce the culture time up to 40-42 h. Peripheral whole-blood samples were exposed to 1-10 Gy of gamma radiation and cultured during 40 and 42 h. No statistical difference between frequencies was obtained between 40, 42 and 48 h of culture time, and PCC index decreased with the increase of the dose and increased with the culture time. The protocol proposed allows reduce the culture time down to 40 or 42 h when using the PCC-R assay with adequate precision in dose estimation.
authors.affiliation: Center of Radiation Protection and Hygiene, Calle 20 No. 4113, Playa, La Habana, Cuba ivonne@cphr.edu.cu.
authors.forename: Ivonne
authors.initials: I
authors.lastname: Romero
authors.affiliation: Center of Radiation Protection and Hygiene, Calle 20 No. 4113, Playa, La Habana, Cuba.
authors.forename: Ana Ilsa
authors.initials: AI
authors.lastname: Lamadrid
authors.affiliation: Center of Radiation Protection and Hygiene, Calle 20 No. 4113, Playa, La Habana, Cuba.
authors.forename: Jorge Ernesto
authors.initials: JE
authors.lastname: González
authors.affiliation: Center of Radiation Protection and Hygiene, Calle 20 No. 4113, Playa, La Habana, Cuba.
authors.forename: Omar
authors.initials: O
authors.lastname: García
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire, BP 17, 92262 Fontenay-aux-Roses Cedex, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Voisin
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire, BP 17, 92262 Fontenay-aux-Roses Cedex, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Roy
mesh_terms: D001681:Biological Assay; D002453:Cell Cycle / Q000235:genetics / Q000528:radiation effects*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000528:radiation effects*; D020732:Cytogenetic Analysis; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D008875:Middle Aged; D013997:Time Factors