title: Evaluation of blood culture media for the detection of fungi.
abstract: The aim of this study was to compare the utility of BACTEC™ Mycosis-IC/F (Mycosis), BACTEC™ Plus Aerobic/F (Aerobic), and BACTEC™ Plus Anaerobic/F (Anaerobic) media in the detection of fungi from simulated (obtained by the inoculation of tested media first with sterile sheep's blood and subsequently with one of 60 clinical yeast isolates) and clinical blood samples, taken during routine diagnostic examination in two hospitals. All tested strains grew on Mycosis as well as Aerobic bottles, and the time to detection obtained for Mycosis was significantly shorter (p < 0.05). The largest differences in the time to positivity was found for Candida glabrata and Cryptococcus neoformans, when Mycosis preceded Aerobic in 20-48 h (mean 35.5 h) and 0.7-64 h (mean 24 h), respectively. On the contrary, C. krusei were detected earlier in Aerobic media. In clinical samples, the detection of C. glabrata was also significantly faster in Mycosis than in Aerobic (29.22 ± 11.48 h compared to 86 ± 40 h). The media complement each other and, in 45% of clinical examination sets, a single positive medium was noted (25% in Mycosis and 19% in Aerobic). The study proved that both Aerobic and Mycosis media serve as the correct condition for the culture of fungi and that they varied significantly in the detection time of clinically important species. This result could suggest that the simultaneous use of Aerobic as well as Mycosis media may improve the time of diagnosis in many patients, especially those infected with C. glabrata or C. neoformans.
authors.affiliation: Department of Microbiology, Medical University of Wrocław, Wrocław, Poland. urszula.nawrot@umed.wroc.pl.
authors.forename: U
authors.initials: U
authors.lastname: Nawrot
authors.affiliation: Department of Microbiology, Medical University of Wrocław, Wrocław, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Kowalska-Krochmal
authors.affiliation: Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Sulik-Tyszka
authors.affiliation: Department of Diagnostics, Laboratory of Microbiology, Military Hospital, Wrocław, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kozak
authors.affiliation: Department of Microbiology, Medical University of Wrocław, Wrocław, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Świętek
authors.affiliation: Department of Microbiology, Medical University of Wrocław, Wrocław, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Pajączkowska
authors.affiliation: Department of Microbiology, Medical University of Wrocław, Wrocław, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Piątkowska
authors.affiliation: Department of Diagnostics, Laboratory of Microbiology, Military Hospital, Wrocław, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Rosiak
authors.affiliation: Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland.|Department of Dental Microbiology, Medical University of Warsaw, Warsaw, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Swoboda-Kopeć
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis*; D013997:Time Factors
chemical_list: D003470:Culture Media
references.citation: Clin Microbiol Infect. 2012 Dec;18 Suppl 7:9-18
references.pmid: 23137134
references.citation: J Clin Microbiol. 2004 Jan;42(1):115-8
references.pmid: 14715740
references.citation: Eur J Clin Microbiol Infect Dis. 2012 Nov;31(11):2983-7
references.pmid: 22669561
references.citation: Mycoses. 2013 Sep;56(5):576-81
references.pmid: 23565662
references.citation: J Hosp Infect. 2003 Nov;55(3):159-68; quiz 233
references.pmid: 14572481
references.citation: Diagn Microbiol Infect Dis. 2012 Apr;72(4):328-31
references.pmid: 22306352
references.citation: J Clin Microbiol. 2005 Jan;43(1):433-5
references.pmid: 15635007
references.citation: Clin Infect Dis. 2004 Aug 1;39(3):309-17
references.pmid: 15306996
references.citation: Mycoses. 2012 May;55(3):228-36
references.pmid: 21777296
references.citation: J Microbiol. 2005 Jun;43(3):257-9
references.pmid: 15995643
references.citation: Eur J Clin Microbiol Infect Dis. 1998 Feb;17(2):113-6
references.pmid: 9629977
references.citation: Clin Infect Dis. 2005 Oct 1;41(7):975-81
references.pmid: 16142662
references.citation: J Clin Microbiol. 2008 Dec;46(12):4029-33
references.pmid: 18923011
references.citation: J Int Med Res. 2008 Nov-Dec;36(6):1261-72
references.pmid: 19094435
references.citation: Mycoses. 2011 Sep;54(5):e539-45
references.pmid: 21605185
references.citation: J Clin Microbiol. 2013 Nov;51(11):3683-7
references.pmid: 23985911
references.citation: J Antimicrob Chemother. 2013 Dec;68(12):2839-41
references.pmid: 23843300
references.citation: J Clin Microbiol. 2011 Sep;49(9):3300-8
references.pmid: 21715585
references.citation: Ann Clin Lab Sci. 2011 Summer;41(3):251-6
references.pmid: 22075508
references.citation: Diagn Microbiol Infect Dis. 2012 Jun;73(2):153-6
references.pmid: 22494558
references.citation: J Clin Microbiol. 2004 Feb;42(2):773-7
references.pmid: 14766852