title: Effect of copper exposure on reproductive ability in the bank vole (Myodes glareolus).
abstract: The amount of copper in natural ecosystems is steadily increasing, due to human activities. It accumulates in plants, posing a threat to herbivores. In polluted areas the population density of small rodents is observed to be lower. The decline in rodent numbers may be caused by increased mortality or diminished fertility. This study examined the effect of copper on the reproductive activity of the bank vole (Myodes glareolus), a small rodent which during foraging often wanders into fields where it might be exposed to pollution. The animals were treated with solutions of 0, 150 or 600 ppm Cu. After 12 weeks of exposure the quality and quantity of the male's sperm was tested. To assess morphological development we compared the experimental groups for body weight, the weight of the male's testes and accessory sex glands, the female's uterus, and the number of matured ovary follicles in tested females. At both doses, copper administration led to lower sperm count and caused sperm head anomalies. The higher dose compromised sperm tail membrane integrity, viability and motility. No effect of copper on morphological development was observed in males, and only the lower dose increased testes weight. In females the higher dose had a negative effect on morphological development, and the lower dose increased uterus weight. No effect of copper on ovarian follicle number was found. For the first time, the morphology of the most typical ovarian follicles of the bank vole is presented.
authors.affiliation: Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland, agata.miska@uj.edu.pl.
authors.forename: Agata
authors.initials: A
authors.lastname: Miska-Schramm
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kruczek
authors.forename: Joanna
authors.initials: J
authors.lastname: Kapusta
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000502:physiology*; D001835:Body Weight / Q000187:drug effects; D003300:Copper / Q000633:toxicity*; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D008297:Male; D009929:Organ Size / Q000187:drug effects; D006080:Ovarian Follicle / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000187:drug effects; D014599:Uterus / Q000187:drug effects
chemical_list: D004785:Environmental Pollutants; D003300:Copper
references.citation: Can J Vet Res. 2001 Apr;65(2):104-10
references.pmid: 11346254
references.citation: Nephron Exp Nephrol. 2010;115(4):e112-21
references.pmid: 20424489
references.citation: J Clin Endocrinol Metab. 1986 Jun;62(6):1184-92
references.pmid: 3084535
references.citation: Ann N Y Acad Sci. 2005 Dec;1061:65-76
references.pmid: 16467258
references.citation: Reprod Toxicol. 2004 Dec;19(2):223-33
references.pmid: 15501388
references.citation: Reproduction. 2003 Nov;126(5):579-88
references.pmid: 14611630
references.citation: J Reprod Fertil. 1986 Jan;76(1):83-9
references.pmid: 3511257
references.citation: Int J Androl. 1985 Apr;8(2):168-74
references.pmid: 4018891
references.citation: Tohoku J Exp Med. 1972 Sep;108(1):9-18
references.pmid: 4648397
references.citation: Reprod Toxicol. 1996 May-Jun;10(3):237-44
references.pmid: 8738562
references.citation: Food Chem Toxicol. 2004 Dec;42(12):2053-60
references.pmid: 15500942
references.citation: Ecotoxicol Environ Saf. 1999 Jul;43(3):261-6
references.pmid: 10381303
references.citation: Biochem Biophys Res Commun. 1983 Apr 15;112(1):306-12
references.pmid: 6404279
references.citation: J Inorg Biochem. 1997 Mar;65(4):277-9
references.pmid: 9046108
references.citation: Endocrinology. 1965 Jun;76:1205-7
references.pmid: 14301530
references.citation: J Nutr. 1973 Feb;103(2):196-201
references.pmid: 4684353
references.citation: Chem Res Toxicol. 1998 Aug;11(8):855-62
references.pmid: 9705746
references.citation: Arch Androl. 1995 Sep-Oct;35(2):111-8
references.pmid: 8579471
references.citation: Acta Vet Scand. 2010 Nov 05;52:58
references.pmid: 21054852
references.citation: Endocrinology. 1977 Aug;101(2):534-9
references.pmid: 328265
references.citation: Reprod Biol Endocrinol. 2005 Sep 27;3:51
references.pmid: 16188042
references.citation: Ginekol Pol. 1994 Feb;65(2):99-102
references.pmid: 8070718
references.citation: Kathmandu Univ Med J (KUMJ). 2005 Oct-Dec;3(4):392-400
references.pmid: 16449843
references.citation: Oecologia. 1994 May;97(4):491-498
references.pmid: 28313738
references.citation: Adv Pharmacol Chemother. 1977;14:327-409
references.pmid: 329660
references.citation: J Toxicol Environ Health A. 2010;73(2):114-27
references.pmid: 20077283
references.citation: Fundam Appl Toxicol. 1993 Nov;21(4):461-75
references.pmid: 8253299
references.citation: Endocrinology. 1965 Dec;77(6):963-7
references.pmid: 5892209
references.citation: Endocrinology. 2003 Jun;144(6):2425-36
references.pmid: 12746304
references.citation: J Reprod Fertil. 1976 Nov;48(2):271-4
references.pmid: 1033278
references.citation: Mutat Res. 2001 Apr 18;475(1-2):141-52
references.pmid: 11295159
references.citation: J Hazard Mater. 2011 Jan 15;185(1):295-305
references.pmid: 20934807
references.citation: Sci Total Environ. 2008 Jan 25;389(2-3):320-8
references.pmid: 17900661
references.citation: Ecotoxicol Environ Saf. 1998 Sep;41(1):2-7
references.pmid: 9756683
references.citation: J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(9):1202-6
references.pmid: 22540640
references.citation: Toxicol Ind Health. 2012 Oct;28(9):789-95
references.pmid: 22042770
references.citation: J Am Coll Nutr. 2003 Jun;22(3):239-46
references.pmid: 12805251
references.citation: Hum Reprod. 2013 Jan;28(1):274-82
references.pmid: 23042799
references.citation: Biol Trace Elem Res. 2006 Sep;112(3):193-203
references.pmid: 17057258
references.citation: Environ Pollut. 2000 Dec;110(3):441-9
references.pmid: 15092823
references.citation: J Nutr. 2003 May;133(5 Suppl 1):1477S-80S
references.pmid: 12730447
references.citation: Reprod Biol. 2007 Nov;7(3):193-205
references.pmid: 18059972
references.citation: Arch Environ Contam Toxicol. 2011 Apr;60(3):524-32
references.pmid: 20532880
references.citation: Arch Environ Contam Toxicol. 2009 Jul;57(1):145-56
references.pmid: 18846312
references.citation: Br J Nutr. 1969 Mar;23(1):47-50
references.pmid: 5766792
references.citation: Neuroendocrinology. 1989 Feb;49(2):197-201
references.pmid: 2498755
references.citation: Environ Sci Pollut Res Int. 2012 Nov;19(9):3977-88
references.pmid: 22532121
references.citation: Environ Int. 2012 Sep;44:53-8
references.pmid: 22336529
references.citation: J Reprod Fertil. 1968 Dec;17(3):555-7
references.pmid: 5715685
references.citation: Biochem Pharmacol. 1986 Sep 1;35(17):2903-9
references.pmid: 3017365
references.citation: Reprod Biol. 2006;6 Suppl 1:201-6
references.pmid: 16967100
references.citation: J Inorg Biochem. 1990 Oct;40(2):121-5
references.pmid: 2092076
references.citation: Behav Processes. 2009 Nov;82(3):279-85
references.pmid: 19635532
references.citation: Zhonghua Nan Ke Xue. 2006 Jul;12(7):590-3
references.pmid: 16894932
references.citation: In Vitro Cell Dev Biol. 1990 Jan;26(1):24-8
references.pmid: 2155192
references.citation: Arch Environ Contam Toxicol. 2007 May;52(4):603-13
references.pmid: 17387425