title: Resveratrol modulates mitochondria dynamics in replicative senescent yeast cells.
abstract: Mitochondria form a reticulum network dynamically fuse and divide in the cell. The balance between mitochondria fusion and fission is correlated to the shape, activity and integrity of these pivotal organelles. Resveratrol is a polyphenol antioxidant that can extend life span in yeast and worm. This study examined mitochondria dynamics in replicative senescent yeast cells as well as the effects of resveratrol on mitochondria fusion and fission. Collecting cells by biotin-streptavidin sorting method revealed that majority of the replicative senescent cells bear fragmented mitochondrial network, indicating mitochondria dynamics favors fission. Resveratrol treatment resulted in a reduction in the ratio of senescent yeast cells with fragmented mitochondria. The readjustment of mitochondria dynamics induced by resveratrol likely derives from altered expression profiles of fusion and fission genes. Our results demonstrate that resveratrol serves not only as an antioxidant, but also a compound that can mitigate mitochondria fragmentation in replicative senescent yeast cells.
authors.affiliation: Institute of Biotechnology, National Tsing Hua University, Hsin Chu City, Taiwan.
authors.forename: I-Hua
authors.initials: IH
authors.lastname: Wang
authors.affiliation: Institute of Biotechnology, National Tsing Hua University, Hsin Chu City, Taiwan.
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Chen
authors.affiliation: Institute of Biotechnology, National Tsing Hua University, Hsin Chu City, Taiwan.
authors.forename: Yu-Han
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Institute of Biotechnology, National Tsing Hua University, Hsin Chu City, Taiwan.
authors.forename: Ko-Wei
authors.initials: KW
authors.lastname: Chang
authors.affiliation: Institute of Biotechnology, National Tsing Hua University, Hsin Chu City, Taiwan.
authors.forename: Ying-Chieh
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Institute of Biotechnology, National Tsing Hua University, Hsin Chu City, Taiwan; Department of Medical Science, National Tsing Hua University, Hsin Chu City, Taiwan.
authors.forename: Chuang-Rung
authors.initials: CR
authors.lastname: Chang
mesh_terms: D063154:Mitochondrial Dynamics / Q000187:drug effects*; D000077185:Resveratrol; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D013267:Stilbenes; D014665:Vasodilator Agents; D000077185:Resveratrol
references.citation: Adv Exp Med Biol. 2012;748:145-69
references.pmid: 22729857
references.citation: J Cell Biol. 2000 Oct 16;151(2):341-52
references.pmid: 11038181
references.citation: J Cell Biol. 1999 Nov 15;147(4):699-706
references.pmid: 10562274
references.citation: Transl Res. 2014 May;163(5):456-65
references.pmid: 24316383
references.citation: J Cell Sci. 2012 Feb 15;125(Pt 4):795-9
references.pmid: 22448035
references.citation: Nature. 2012 Dec 13;492(7428):261-5
references.pmid: 23172144
references.citation: Muscle Nerve. 2013 Dec;48(6):963-70
references.pmid: 23494933
references.citation: Nat Cell Biol. 2011 May;13(5):521-2
references.pmid: 21540850
references.citation: Science. 2012 Nov 9;338(6108):822-4
references.pmid: 23139336
references.citation: Mol Biol Cell. 2011 Apr 15;22(8):1207-16
references.pmid: 21325626
references.citation: J Cell Biol. 2000 Oct 16;151(2):367-80
references.pmid: 11038183
references.citation: J Biol Chem. 2004 Jul 2;279(27):28298-303
references.pmid: 15087460
references.citation: Nature. 2003 Sep 11;425(6954):191-6
references.pmid: 12939617
references.citation: Cell. 2011 Sep 2;146(5):682-95
references.pmid: 21884931
references.citation: Free Radic Biol Med. 2011 Apr 15;50(8):963-70
references.pmid: 21255640
references.citation: J Cell Biol. 2005 Jul 18;170(2):237-48
references.pmid: 16009724
references.citation: Cell. 1997 May 2;89(3):381-91
references.pmid: 9150138
references.citation: J Cell Biol. 2006 Jun 5;173(5):645-50
references.pmid: 16735578
references.citation: Nature. 2011 Sep 21;477(7365):E1-2
references.pmid: 21938026
references.citation: Eur J Clin Invest. 2013 Aug;43(8):886-93
references.pmid: 23869410
references.citation: Nat Cell Biol. 2011 May;13(5):589-98
references.pmid: 21478857
references.citation: Mutat Res. 1992 Sep;275(3-6):181-93
references.pmid: 1383760
references.citation: Adv Exp Med Biol. 2012;942:311-27
references.pmid: 22399429
references.citation: J Cell Biol. 2000 Oct 16;151(2):353-66
references.pmid: 11038182
references.citation: Science. 2012 Aug 31;337(6098):1062-5
references.pmid: 22936770
references.citation: Cell. 1997 Dec 26;91(7):1033-42
references.pmid: 9428525
references.citation: Biochim Biophys Acta. 2014 Jul;1842(7):902-15
references.pmid: 24582596
references.citation: J Biol Chem. 1998 Aug 7;273(32):20150-5
references.pmid: 9685359
references.citation: Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):E2967-76
references.pmid: 23878239
references.citation: Curr Opin Cell Biol. 2014 Feb;26:34-40
references.pmid: 24529244
references.citation: Biochim Biophys Acta. 2013 Jan;1832(1):216-27
references.pmid: 23046812
references.citation: Semin Cell Dev Biol. 2010 Aug;21(6):542-9
references.pmid: 20006727
references.citation: J Biol Chem. 2007 Jul 27;282(30):21583-7
references.pmid: 17553808
references.citation: J Biol Chem. 2007 Aug 3;282(31):22977-83
references.pmid: 17545159
references.citation: Nat Cell Biol. 1999 Sep;1(5):298-304
references.pmid: 10559943
references.citation: FASEB J. 2009 Nov;23(11):3917-27
references.pmid: 19638400
references.citation: Annu Rev Genet. 2005;39:503-36
references.pmid: 16285870
references.citation: J Cell Biol. 1998 Oct 19;143(2):359-73
references.pmid: 9786948
references.citation: Mycologia. 2005 May-Jun;97(3):580-8
references.pmid: 16392246
references.citation: J Cell Sci. 2007 Apr 1;120(Pt 7):1178-88
references.pmid: 17341580
references.citation: Cell Metab. 2012 Jul 3;16(1):18-31
references.pmid: 22768836
references.citation: Science. 2009 Apr 3;324(5923):102-5
references.pmid: 19342591
references.citation: Mol Microbiol. 2001 Mar;39(5):1166-73
references.pmid: 11251834
references.citation: Nature. 2004 Aug 5;430(7000):686-9
references.pmid: 15254550
references.citation: J Cell Biol. 2007 May 7;177(3):439-50
references.pmid: 17470634
references.citation: Essays Biochem. 2010;47:85-98
references.pmid: 20533902
references.citation: J Cell Physiol. 2006 Nov;209(2):468-80
references.pmid: 16883569
references.citation: Biochem Soc Trans. 2011 Oct;39(5):1520-6
references.pmid: 21936845
references.citation: Genetics. 2009 Oct;183(2):413-22, 1SI-13SI
references.pmid: 19652178
references.citation: EMBO Rep. 2010 Sep;11(9):678-84
references.pmid: 20725092
references.citation: Diabetes. 2010 Mar;59(3):554-63
references.pmid: 19934007
references.citation: Yeast. 2000 Nov;16(15):1421-7
references.pmid: 11054823
references.citation: Mutat Res. 1992 Sep;275(3-6):169-80
references.pmid: 1383759
references.citation: Cell. 2010 Apr 16;141(2):280-9
references.pmid: 20403324