title: Effect of ABCB1 polymorphism on the clinical outcome of osteosarcoma patients after receiving chemotherapy.
abstract: OBJECTIVE
  To investigate the role of three genetic polymorphisms of ABC proteins in response to chemotherapy and overall survival of osteosarcoma patients.
  
  
  METHODS
  A prospective study was conducted. Genotyping analyses of ABCB1 C3435T, ABCG2 C421A, and ABCC3 C-211T were conducted using the TaqMan methodology. Multivariate Cox proportional hazards models were used to calculate hazard ratio (HR) and 95% CI of effect of each genotype of ABCB1 C3435T, ABCG2 c421A, and ABCC3 C-211T on PFS and OS.
  
  
  RESULTS
  During the follow-up period, 135 patients (74.18%) were alive and 47 died (25.82). The median follow-up periods were 36.7 months. Individuals carrying with ABCB1 3435TT genotype and T allele showed less likely to have a poor response to chemotherapy. Cox regression analysis showed that individuals with ABCB1 TT genotype and T allele were associated with high risk of death from osteosarcoma when compared with wide-type genotype. However, we did not find significant association between ABCG2 C421A and ABCC3 C-211T polymorphisms and overall survival of osteosarcoma.
  
  
  CONCLUSIONS
  ABCB1 C3435T polymorphism may be used as a genetic predictor of clinical outcome in osteosarcoma patients treated with chemotherapy. However, no association was found between polymorphisms in ABCG2 C421A and ABCC3 C-211T and clinical outcome of osteosarcoma.
authors.affiliation: Sun Xiaohui, Department of Orthopedic Surgery, The first affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Xiaohui
authors.affiliation: Li Aiguo, Department of Orthopedic Surgery, The first affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Aiguo
authors.affiliation: Geng Xiaolin, Department of Orthopedic Surgery, The first affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China.
authors.forename: Geng
authors.initials: G
authors.lastname: Xiaolin
authors.affiliation: Liu Ying, Department of Obstetrics and Gynecology, The first affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Ying
authors.affiliation: Zhao Hongxing, Department of Orthopedic Surgery, The first affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Hongxing
authors.affiliation: Zhao Yilei, Department of Orthopedic Surgery, The first affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yilei
keywords: APT-binding cassette; Chemotherapy; Clinical outcome; Osteosarcoma
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