title: Expression profile and clinical significance of microRNAs in papillary thyroid carcinoma.
abstract: This study screened microRNAs (miRNAs) that are abnormally expressed in papillary thyroid carcinoma (PTC) tissues to identify PTC and nodular goiter and the degree of PTC malignancy. A total of 51 thyroid tumor tissue specimens paired with adjacent normal thyroid tissues were obtained from the Department of Surgical Oncology of Hangzhou First People's Hospital from June-December 2011. miRNA expression profiles were examined by microarrays and validated by quantitative real-time PCR (qRT-PCR). Expression levels of the miRNAs were analyzed to assess if they were associated with selected clinicopathological features. Eleven miRNAs were significantly differentially expressed between nodular goiter and PTC and between highly invasive and low invasive PTC. miR-199b-5p and miR-30a-3p were significantly differentially expressed among the three groups. miR-30a-3p, miR-122-5p, miR-136-5p, miR-146b-5p and miR-199b-5p were selected for further study by qRT-PCR and miR-146b-5p, miR-199b-5p and miR-30a-3p were different between the PTC and nodular goiter groups. miR-199b-5p was over-expressed in PTC patients with extrathyroidal invasion and cervical lymph node metastasis. In conclusion miR-146b-5p, miR-30a-3p, and miR-199b-5p may serve as biomarkers for the diagnosis of PTC and miR-199b-5p is associated with PTC invasiveness.
authors.affiliation: Department of Oncological Surgery, Hangzhou First People's Hospital, Hangzhou 310006, China. doctor3663@163.com.
authors.forename: You
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of General Surgery, Wuxi Second People's Hospital, Wuxi 214000, China. bullfrog007@sina.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Oncological Surgery, Wushan District of Hangzhou First People's Hospital, Hangzhou Cancer Hospital, Hangzhou 310002, China. ldc65@163.com.
authors.forename: Ding-Cun
authors.initials: DC
authors.lastname: Luo
authors.affiliation: Department of Oncological Surgery, Hangzhou First People's Hospital, Hangzhou 310006, China. zjlsdjw@163.com.
authors.forename: Jin-Wang
authors.initials: JW
authors.lastname: Ding
authors.affiliation: Department of Oncological Surgery, Hangzhou First People's Hospital, Hangzhou 310006, China. zwwyntx@sina.com.
authors.forename: Wo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Oncological Surgery, Hangzhou First People's Hospital, Hangzhou 310006, China. xjxsh@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Pan
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000175:diagnosis / Q000235:genetics*; D002291:Carcinoma, Papillary; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006044:Goiter, Nodular / Q000175:diagnosis / Q000235:genetics; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D015203:Reproducibility of Results; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000235:genetics*; D059467:Transcriptome; D047368:Tumor Burden
chemical_list: D035683:MicroRNAs
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