title: Role of acrAB in antibiotic resistance of Haemophilus parasuis serovar 4.
abstract: Haemophilus parasuis infection is responsible for important economic losses to the pig industry. The increasing emergence of resistance to multiple antibiotics is of concern and to study the role of the acrRAB operon in H. parasuis drug resistance, acrB or acrR mutants were generated from H. parasuis serovar 4 clinical strains. The susceptibilities of the clinical strains and their acrB/acrR mutants to a number of antibiotics were determined. The acrB mutants were more susceptible to novobiocin, erythromycin, clarithromycin, and azithromycin. In the acrR mutant of H. parasuis, acrB was up-regulated, as determined by quantitative reverse transcriptase polymerase chain reaction. The results of this study indicated that the efflux pump AcrB may play a role in multidrug resistance of H. parasuis.
authors.affiliation: Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510624, China.
authors.forename: Saixiang
authors.initials: S
authors.lastname: Feng
authors.affiliation: Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510624, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510624, China.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510624, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Fan
authors.affiliation: Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510624, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
authors.affiliation: Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510624, China. Electronic address: rentao6868@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Ren
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D044137:Haemophilus parasuis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins
keywords: AcrAB efflux pump; Drug resistance; Haemophilus parasuis