title: Distinct immunomodulation of bone marrow-derived dendritic cell responses to Lactobacillus plantarum WCFS1 by two different polysaccharides isolated from Lactobacillus rhamnosus LOCK 0900.
abstract: The structures of polysaccharides (PS) isolated from Lactobacillus rhamnosus LOCK 0900 and results from stimulation of mouse bone marrow-derived dendritic cells (BM-DC) and human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR) upon exposure to these antigens were studied. L. rhamnosus LOCK 0900 produces PS that differ greatly in their structure. The polymer L900/2, with a high average molecular mass of 830 kDa, is a branched heteropolysaccharide with a unique repeating unit consisting of seven sugar residues and pyruvic acid, whereas L900/3 has a low average molecular mass of 18 kDa and contains a pentasaccharide repeating unit and phosphorus. Furthermore, we found that both described PS neither induce cytokine production and maturation of mouse BM-DC nor induce signaling through TLR2/TLR4 receptors. However, they differ profoundly in their abilities to modulate the BM-DC immune response to the well-characterized human isolate Lactobacillus plantarum WCFS1. Exposure to L900/2 enhanced interleukin-10 (IL-10) production induced by L. plantarum WCFS1, while in contrast, L900/3 enhanced the production of IL-12p70. We conclude that PS, probably due to their chemical features, are able to modulate the immune responses to third-party antigens. The ability to induce regulatory IL-10 by L900/2 opens up the possibility to use this PS in therapy of inflammatory conditions, such as inflammatory bowel disease, whereas L900/3 might be useful in reverting the antigen-dependent Th2-skewed immune responses in allergies.
authors.affiliation: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland sabina.gorska@iitd.pan.wroc.pl schwarzer@biomed.cas.cz.
authors.forename: Sabina
authors.initials: S
authors.lastname: Górska
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Gnotobiology, Novy Hradek, Czech Republic sabina.gorska@iitd.pan.wroc.pl schwarzer@biomed.cas.cz.
authors.forename: Martin
authors.initials: M
authors.lastname: Schwarzer
authors.affiliation: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Jachymek
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Gnotobiology, Novy Hradek, Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Srutkova
authors.affiliation: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Brzozowska
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Gnotobiology, Novy Hradek, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Kozakova
authors.affiliation: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002236:Carbohydrate Conformation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D057809:HEK293 Cells / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology*; D052201:Lacticaseibacillus rhamnosus / Q000737:chemistry* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D019936:Probiotics; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
references.citation: Carbohydr Res. 1980 Mar;79(2):313-5
references.pmid: 7357572
references.citation: Carbohydr Res. 1979 Dec;77:10-7
references.pmid: 519653
references.citation: Microbiology (Reading). 2005 Jun;151(Pt 6):1839-1851
references.pmid: 15941992
references.citation: Carbohydr Res. 1989 Jun 1;188:169-91
references.pmid: 2673508
references.citation: Allergy. 2009 Mar;64(3):406-14
references.pmid: 19120072
references.citation: Genome Announc. 2013 Aug 15;1(4):
references.pmid: 23950137
references.citation: Mol Microbiol. 2010 Sep;77(6):1439-55
references.pmid: 20633227
references.citation: Nat Rev Gastroenterol Hepatol. 2014 Aug;11(8):506-14
references.pmid: 24912386
references.citation: J Sci Food Agric. 2011 Sep;91(12):2284-91
references.pmid: 21560134
references.citation: J Appl Microbiol. 2010 Dec;109(6):2079-86
references.pmid: 20846331
references.citation: Cell Mol Immunol. 2011 Mar;8(2):110-20
references.pmid: 21278760
references.citation: J Exp Med. 1980 Oct 1;152(4):979-95
references.pmid: 6158553
references.citation: Biochem J. 2002 Apr 1;363(Pt 1):7-17
references.pmid: 11903041
references.citation: J Dairy Sci. 2006 Aug;89(8):2873-81
references.pmid: 16840603
references.citation: Carbohydr Res. 2012 Jul 1;355:56-62
references.pmid: 22578768
references.citation: Int J Syst Evol Microbiol. 2007 Dec;57(Pt 12):2777-2789
references.pmid: 18048724
references.citation: Carbohydr Res. 1981 Sep 1;95(1):73-80
references.pmid: 7285020
references.citation: Arch Immunol Ther Exp (Warsz). 2012 Jun;60(3):211-20
references.pmid: 22484803
references.citation: J Appl Microbiol. 2010 Feb;108(2):666-75
references.pmid: 19702865
references.citation: Allergy. 2011 Mar;66(3):368-75
references.pmid: 20880132
references.citation: Vaccine. 2003 Dec 8;22(1):87-95
references.pmid: 14604575
references.citation: Nat Rev Microbiol. 2010 Mar;8(3):171-84
references.pmid: 20157338
references.citation: J Am Chem Soc. 1977 May 25;99(11):3812-5
references.pmid: 858877
references.citation: Trends Microbiol. 2011 Jul;19(7):349-59
references.pmid: 21684749
references.citation: Cytokine. 2006 Dec;36(5-6):254-60
references.pmid: 17363262
references.citation: Gut Microbes. 2012 Sep-Oct;3(5):420-5
references.pmid: 22713271
references.citation: Mediators Inflamm. 2013;2013:237921
references.pmid: 23576850
references.citation: Mol Immunol. 2004 Nov;41(11):1099-108
references.pmid: 15476921
references.citation: Clin Exp Immunol. 2007 Dec;150(3):531-8
references.pmid: 17956582
references.citation: Pharmacol Res. 2013 Mar;69(1):61-74
references.pmid: 23059538
references.citation: Probiotics Antimicrob Proteins. 2012 Dec;4(4):227-37
references.pmid: 26782182
references.citation: Carbohydr Res. 2013 Aug 30;378:148-53
references.pmid: 23787056
references.citation: Postepy Hig Med Dosw (Online). 2007 Dec 14;61:805-18
references.pmid: 18097339
references.citation: Anaerobe. 2014 Apr;26:24-30
references.pmid: 24445155
references.citation: Carbohydr Polym. 2014 Jul 17;107:51-6
references.pmid: 24702917
references.citation: J Bacteriol. 1971 Jul;107(1):150-4
references.pmid: 4998246
references.citation: Int J Biol Macromol. 2014 Jun;67:71-8
references.pmid: 24631548
references.citation: Int J Biol Macromol. 2014 Jul;68:233-40
references.pmid: 24820155
references.citation: J Food Prot. 2006 Aug;69(8):2011-5
references.pmid: 16924934
references.citation: Science. 2013 Oct 25;342(6157):447-53
references.pmid: 24072822
references.citation: Antonie Van Leeuwenhoek. 2009 Nov;96(4):505-13
references.pmid: 19633916
references.citation: Carbohydr Polym. 2014 May 25;105:334-40
references.pmid: 24708988
references.citation: Biochem J. 1969 Dec;115(5):947-58
references.pmid: 4902692
references.citation: J Atheroscler Thromb. 2010 Sep 30;17(9):980-8
references.pmid: 20543518
references.citation: Carbohydr Res. 1988 Apr 1;175(1):59-75
references.pmid: 3378242
references.citation: Carbohydr Res. 2006 Apr 10;341(5):616-23
references.pmid: 16442512
references.citation: Folia Microbiol (Praha). 2010 May;55(3):277-80
references.pmid: 20526842