title: Characterization of newly isolated lytic bacteriophages active against Acinetobacter baumannii.
abstract: Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB_AbaM_Acibel004 and podovirus vB_AbaP_Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively), high burst size (125 and 145, respectively), stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.
authors.affiliation: Laboratory for Molecular and Cellular Technology (LabMCT), Queen Astrid Military Hospital, Brussels, Belgium; Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia; Laboratory for Bacteriology Research (LBR), Faculty Medicine & Health Sciences, Ghent University, Ghent, Belgium.
authors.forename: Maia
authors.initials: M
authors.lastname: Merabishvili
authors.affiliation: Laboratory of Gene Technology, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Dieter
authors.initials: D
authors.lastname: Vandenheuvel
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.affiliation: Electron Microscopy Unit, Veterinary and Agrochemical Research Centre, Brussels, Belgium.
authors.forename: Jan
authors.initials: J
authors.lastname: Mast
authors.affiliation: Laboratory for Molecular and Cellular Technology (LabMCT), Queen Astrid Military Hospital, Brussels, Belgium.
authors.forename: Daniel
authors.initials: D
authors.lastname: De Vos
authors.affiliation: Laboratory for Molecular and Cellular Technology (LabMCT), Queen Astrid Military Hospital, Brussels, Belgium.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Verbeken
authors.affiliation: Department of Pathology, Bacteriology and Poultry Diseases, Ghent University, Merelbeke, Belgium.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Noben
authors.affiliation: Laboratory of Gene Technology, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
authors.affiliation: Laboratory for Bacteriology Research (LBR), Faculty Medicine & Health Sciences, Ghent University, Ghent, Belgium.
authors.forename: Mario
authors.initials: M
authors.lastname: Vaneechoutte
authors.affiliation: Laboratory for Molecular and Cellular Technology (LabMCT), Queen Astrid Military Hospital, Brussels, Belgium.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Pirnay
mesh_terms: D040981:Acinetobacter baumannii / Q000821:virology*; D001435:Bacteriophages / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology*; D046508:Culture Techniques; D016679:Genome, Viral / Q000235:genetics; D058507:Host Specificity; D058977:Molecular Sequence Annotation; D010641:Phenotype; D040901:Proteomics
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