title: Troponin T immunosensor based on liquid crystal and silsesquioxane-supported gold nanoparticles.
abstract: A nanostructured immunosensor based on the liquid crystal (E)-1-decyl-4-[(4-decyloxyphenyl)diazenyl]pyridinium bromide (Br-Py) and gold nanoparticles supported by the water-soluble hybrid material 3-n-propyl-4-picolinium silsesquioxane chloride (AuNP-Si4Pic(+)Cl(-)) was built for the detection of troponin T (cTnT), a cardiac marker for acute myocardial infarction (AMI). The functionalized nanostructured surface was used to bind anti-cTnT monoclonal antibodies through electrostatic interaction. The immunosensor (ab-cTnT/AuNP-Si4Pic(+)Cl(-)/Br-Py/GCE) surface was characterized by microscopy techniques. The electrochemical behavior of the immunosensor was studied by cyclic voltammetry and electrochemical impedance spectroscopy. A calibration curve was obtained by square-wave voltammetry. The immnunosensor provided a limit of detection of 0.076 ng mL(-1) and a linear range between 0.1 and 0.9 ng mL(-1) (appropriate for AMI diagnosis).
authors.affiliation: Laboratory of Biosensors, ‡Study Group of Electrochemical and Electroanalytical Processes, and §Laboratory of Synthesis of Liquid Crystals, Department of Chemistry, Federal University of Santa Catarina , 88040-900, Florianópolis, SC, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Zapp
authors.forename: Paulo Sérgio
authors.initials: PS
authors.lastname: da Silva
authors.forename: Eduard
authors.initials: E
authors.lastname: Westphal
authors.forename: Hugo
authors.initials: H
authors.lastname: Gallardo
authors.forename: Almir
authors.initials: A
authors.lastname: Spinelli
authors.forename: Iolanda Cruz
authors.initials: IC
authors.lastname: Vieira
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D050866:Liquid Crystals / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D020107:Troponin T / Q000032:analysis*
chemical_list: C000598101:3-n-propyl-4-picolinium silsesquioxane chloride; D017646:Organosilicon Compounds; D011726:Pyridinium Compounds; D020107:Troponin T; D006046:Gold