title: 68Ga-labeled cyclic NGR peptide for microPET imaging of CD13 receptor expression.
abstract: Peptides containing the asparagines-glycine-arginine (NGR) motif have been identified as specific ligands binding to CD13/aminopeptidase N (APN) receptor, a tumor neovascular biomarker. In this study, we synthesized a novel NGR-containing peptide (NOTA-G3-NGR), and labeled NOTA-G3-NGR with (68)Ga (t1/2 = 67.7 min). The resulting (68)Ga-NOTA-G3-NGR peptide was subject to in vitro and in vivo characterization. The microPET imaging results revealed that the (68)Ga-NOTA-G3-NGR peptide exhibits rapid and specific tumor uptake, and high tumor-to-background contrast in a subcutaneous HT-1080 fibrosarcoma mouse model. We concluded that the (68)Ga-NOTA-G3-NGR peptide has potential in the diagnosis of CD13-targeted tumor angiogenesis.
authors.affiliation: Department of Nuclear Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Department of Nuclear Medicine, Lanzhou General Hospital of Lanzhou Military Area Command, Lanzhou 730050, China.
authors.forename: Wansheng
authors.initials: W
authors.lastname: Liang
authors.affiliation: Department of Nuclear Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Kang
authors.affiliation: Department of Nuclear Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Nuclear Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Nuclear Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Guiyu
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Nuclear Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zong
authors.affiliation: Molecular Imaging Center, Department of Radiology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. chenkai@usc.edu.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Nuclear Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China. wangjing@fmmu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000626:Aminopeptidases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D018826:CD13 Antigens / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D005710:Gallium Radioisotopes / Q000737:chemistry; D015870:Gene Expression; D064593:Heterografts; D006801:Humans; D051379:Mice; D057054:Molecular Imaging; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D049268:Positron-Emission Tomography; D011485:Protein Binding; D059629:Signal-To-Noise Ratio; D014018:Tissue Distribution
chemical_list: D005710:Gallium Radioisotopes; C526428:NGR peptide; D009842:Oligopeptides; D000626:Aminopeptidases; D018826:CD13 Antigens
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