title: Thalassotalea ponticola sp. nov., isolated from seawater, reclassification of Thalassomonas fusca as Thalassotalea fusca comb. nov. and emended description of the genus Thalassotalea.
abstract: A Gram-stain-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated GJSW-36(T), was isolated from seawater at Geoje island in the South Sea, South Korea. Strain GJSW-36(T) grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain GJSW-36(T) fell within the clade comprising the type strains of species of the genus Thalassotalea and Thalassomonas fusca. Strain GJSW-36(T) exhibited 16S rRNA gene sequence similarity values of 94.2-96.0% to the type strains of species of the genus Thalassotalea and Thalassomonas fusca and of 93.8-94.5% to the type strains of the other species of the genus Thalassomonas. Strain GJSW-36(T) contained ubiquinone-8 (Q-8) as the predominant ubiquinone and summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c), C(1:1)ω8c and C(16:0) as the major fatty acids. The major polar lipids of strain GJSW-36(T) were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain GJSW-36(T) was 45.1 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain GJSW-36(T) is separated from species of the genus Thalassotalea and Thalassomonas fusca. On the basis of the data presented, strain GJSW-36(T) is considered to represent a novel species of the genus Thalassotalea, for which the name Thalassotalea ponticola sp. nov. is proposed. The type strain is GJSW-36(T) ( =KCTC 42155(T) =CECT 8656(T)). From this study, it is also proposed that Thalassomonas fusca should be reclassified as a member of the genus Thalassotalea and the description of the genus Thalassotalea is emended.
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Chul-Hyung
authors.initials: CH
authors.lastname: Kang
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Ja-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C483858:phosphatidylethanolamine; C030778:ubiquinone 8