title: Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains.
abstract: The objective of this study was to isolate, identify, and characterize some lactic acid bacterial strains from human milk, infant feces, and fermented grapes and dates, as potential probiotics with antimicrobial activity against some human pathogenic strains. One hundred and forty bacterial strains were isolated and, after initial identification and a preliminary screening for acid and bile tolerance, nine of the best isolates were selected and further identified using 16 S rRNA gene sequences. The nine selected isolates were then characterized in vitro for their probiotic characteristics and their antimicrobial activities against some human pathogens. Results showed that all nine isolates belonged to the genus Lactobacillus. They were able to tolerate pH 3 for 3 h, 0.3% bile salts for 4 h, and 1.9 mg/mL pancreatic enzymes for 3 h. They exhibited good ability to attach to intestinal epithelial cells and were not resistant to the tested antibiotics. They also showed good antimicrobial activities against the tested pathogenic strains of humans, and most of them exhibited stronger antimicrobial activity than the reference strain L. casei Shirota. Thus, the nine Lactobacillus strains could be considered as potential antimicrobial probiotic strains against human pathogens and should be further studied for their human health benefits.
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Parisa
authors.initials: P
authors.lastname: Shokryazdan
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia ; Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Chin Chin
authors.initials: CC
authors.lastname: Sieo
authors.affiliation: Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia.
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Kalavathy
authors.affiliation: Institute of Tropical Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Juan Boo
authors.initials: JB
authors.lastname: Liang
authors.affiliation: Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Noorjahan Banu
authors.initials: NB
authors.lastname: Alitheen
authors.affiliation: Institute of Tropical Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Faseleh Jahromi
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
authors.forename: Yin Wan
authors.initials: YW
authors.lastname: Ho
mesh_terms: D000328:Adult; D000890:Anti-Infective Agents; D001422:Bacterial Adhesion / Q000502:physiology; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007778:Lactobacillus / Q000145:classification / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology; D008895:Milk, Human / Q000382:microbiology; D019936:Probiotics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000890:Anti-Infective Agents; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
references.citation: Oral Microbiol Immunol. 2008 Apr;23(2):139-47
references.pmid: 18279182
references.citation: Meat Sci. 2008 Nov;80(3):715-21
references.pmid: 22063588
references.citation: Appl Environ Microbiol. 1995 Jan;61(1):116-21
references.pmid: 7887594
references.citation: Bioinformatics. 2004 Sep 22;20(14):2317-9
references.pmid: 15073015
references.citation: Acta Physiol Scand. 1959 Aug 31;46:339-45
references.pmid: 13831440
references.citation: Curr Opin Biotechnol. 2009 Apr;20(2):135-41
references.pmid: 19243931
references.citation: J Appl Microbiol. 2002;92(5):966-75
references.pmid: 11972703
references.citation: Int J Food Microbiol. 1998 Oct 20;44(1-2):93-106
references.pmid: 9849787
references.citation: J Dairy Sci. 1984 Dec;67(12):3045-51
references.pmid: 6442304
references.citation: J Dairy Sci. 2005 Jan;88(1):55-66
references.pmid: 15591367
references.citation: Aliment Pharmacol Ther. 2003 Feb;17(3):429-35
references.pmid: 12562457
references.citation: Appl Environ Microbiol. 2005 Dec;71(12):7724-36
references.pmid: 16332745
references.citation: Res Microbiol. 2003 Dec;154(10):705-12
references.pmid: 14643409
references.citation: Int J Food Microbiol. 2010 Nov 15;144(1):81-7
references.pmid: 20888656
references.citation: Mol Biol Evol. 1992 Jul;9(4):678-87
references.pmid: 1630306
references.citation: Appl Environ Microbiol. 2008 Jun;74(12):3745-8
references.pmid: 18441109
references.citation: Int J Food Microbiol. 1998 May 26;41(2):103-25
references.pmid: 9704860
references.citation: Lett Appl Microbiol. 1998 Sep;27(3):183-5
references.pmid: 9750324
references.citation: Int J Food Microbiol. 1998 May 5;41(1):45-51
references.pmid: 9631336
references.citation: Am J Clin Nutr. 2001 Feb;73(2 Suppl):386S-392S
references.pmid: 11157346
references.citation: Appl Environ Microbiol. 1999 Nov;65(11):4949-56
references.pmid: 10543808
references.citation: Appl Microbiol Biotechnol. 1998 Aug;50(2):253-6
references.pmid: 9763693
references.citation: J Appl Microbiol. 1998 May;84(5):759-68
references.pmid: 9674129
references.citation: Vet Med (Praha). 1997 Jan;42(1):19-27
references.pmid: 9123779
references.citation: Infect Immun. 2006 Jan;74(1):425-34
references.pmid: 16368998
references.citation: J Appl Microbiol. 2003;95(5):1058-69
references.pmid: 14633035
references.citation: Microbiol Res. 1997 Jul;152(2):171-9
references.pmid: 9265768
references.citation: Public Health Nutr. 1999 Jun;2(2):223-9
references.pmid: 10447251
references.citation: J Nutr Biochem. 2003 May;14(5):259-65
references.pmid: 12832029
references.citation: Int J Food Microbiol. 1999 Mar 1;47(1-2):79-87
references.pmid: 10357276
references.citation: BMJ. 1996 Jan 6;312(7022):55-6
references.pmid: 8555882
references.citation: PLoS One. 2012;7(9):e43928
references.pmid: 22962594
references.citation: Benef Microbes. 2010 Mar;1(1):75-80
references.pmid: 21831752
references.citation: Appl Environ Microbiol. 2005 Dec;71(12):8982-6
references.pmid: 16332905
references.citation: Dig Liver Dis. 2006 Dec;38 Suppl 2:S248-55
references.pmid: 17259085
references.citation: FEMS Microbiol Rev. 1999 Apr;23(2):153-77
references.pmid: 10234843
references.citation: Int J Food Microbiol. 2001 Aug 5;67(3):207-16
references.pmid: 11518430
references.citation: Biomed Res Int. 2014;2014:208974
references.pmid: 24955346
references.citation: Int J Food Microbiol. 2003 May 25;83(1):63-74
references.pmid: 12672593