title: Double substituted variant of Bacillus amyloliquefaciens esterase with enhanced enantioselectivity and high activity towards 1-(3',4'-methylenedioxyphenyl)ethyl acetate.
abstract: Bacillus amyloliquefaciens esterase (BAE) was applied to produce (R)-1-(3',4'-methylenedioxyphenyl)ethanol, a chiral drug intermediate. In this study, we improved the enantioselectivity of BAE by protein engineering instead of process engineering as used in our previous work. Saturation mutagenesis was carried out on eight positions of BAE based on structure modeling and substrate docking. A double substituted variant V10 (K358D/A396C) showed an excellent enantioselectivity without decreasing the activity. The functions of these two mutations (K358D and A396C) were investigated, revealing a synergic effect on the BAE enantioselectivity. Using the variant V10, enantiopure (R)-1-(3',4'-methylenedioxyphenyl)ethanol could be readily prepared in >97 % ee, affording a high space-time yield (123 g L(-1) day(-1)) and a high ratio of substrate/catalyst (40 g g(-1)) in 1-L reaction.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
authors.forename: Jia-Yan
authors.initials: JY
authors.lastname: Liu
authors.forename: Han-Ping
authors.initials: HP
authors.lastname: Bian
authors.forename: Yun
authors.initials: Y
authors.lastname: Tang
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Bai
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Xu
mesh_terms: D000085:Acetates / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D004252:DNA Mutational Analysis; D004950:Esterases / Q000235:genetics / Q000378:metabolism*; D016296:Mutagenesis; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D000085:Acetates; D050505:Mutant Proteins; C007650:ethyl acetate; D004950:Esterases