title: Transforming growth factor-beta polymorphisms and serum level in the development of osteosarcoma.
abstract: The transforming growth factor-beta (TGF-β) signaling pathway plays critical roles in the development of various diseases. The current study investigated whether TGF-β was involved in the pathogenesis of osteosarcoma from the genetic polymorphism perspective and serum level perspective. We first examined two TGF-β1 polymorphisms, rs1800469C/T and rs1800470T/C, in 202 osteosarcoma patients and 216 healthy controls. Data revealed that the prevalence of rs1800470TT genotype and T allele was significantly elevated in patients than in controls (odds ratio [OR]=2.28, 95% confidence interval [CI]: 1.30-3.98, p<0.001, and OR=1.49, 95% CI: 1.14-1.96, p<0.001). Function analyses showed that healthy controls carrying rs1800470TT genotype had a significantly higher serum level of TGF-β than those carrying the rs1800470CC genotype (191.1±15.7 pg/mL vs. 129.4±10.9 pg/mL, p=0.003). We then compared the serum level of TGF-β between osteosarcoma patients and healthy controls. Results demonstrated a significantly increased serum level of TGF-β in patients than in controls. Further analyses identified that patients with metastasis had augmented levels of serum TGF-β than those without metastasis. These data indicate that TGF-β may be closely involved in the pathogenesis of osteosarcoma.
authors.affiliation: 1 Department of Orthopedics, Zhengzhou Orthopedics Hospital , Zhengzhou, Henan, China .
authors.forename: Shaogang
authors.initials: S
authors.lastname: Xu
authors.forename: Shufeng
authors.initials: S
authors.lastname: Yang
authors.forename: Guojing
authors.initials: G
authors.lastname: Sun
authors.forename: Wanxin
authors.initials: W
authors.lastname: Huang
authors.forename: Yelong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000235:genetics*; D055815:Young Adult
chemical_list: D053773:Transforming Growth Factor beta1