title: Urinary carbonic anhydrase VI as a biomarker for kidney disease in pigs.
abstract: This study investigated whether carbonic anhydrase (CA)-VI has utility as a biomarker in swine kidney disease. Serum chemistry, histopathology, immunohistochemical staining and enzyme-linked immunosorbent assay (ELISA) analyses were performed. In the kidney of normal healthy pigs, CA-VI was localized in the epithelial cells of the renal distal straight tubules. CA-VI levels were 16 ± 35 ng/g wet tissue and 50 ± 66 ng/mL in normal pig kidney and urine, respectively, and 136 ± 173 ng/mL in the urine of pigs with kidney disease. CA-VI urinary concentration was not correlated with urinary urea nitrogen (UUN), urinary creatinine (Cre), or urinary albumin levels in pigs with kidney disease. However, UUN and Cre levels were positively correlated in the urine of pigs with kidney disease. These data suggest that urinary CA-VI may represent a biomarker for kidney disease in pigs, particularly for disorders affecting distal straight tubules.
authors.affiliation: Laboratory of Physiology I, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan. Electronic address: nishida@azabu-u.ac.jp.
authors.forename: Toshiho
authors.initials: T
authors.lastname: Nishita
authors.affiliation: Miyagi Prefectural Meat Sanitation Inspection Station, 314 Imaizumi, Sakuraoka, Yoneyamacho, Tome City, Miyagi 987-0031, Japan.
authors.forename: Juro
authors.initials: J
authors.lastname: Yatsu
authors.affiliation: Piglet Co., Ltd., 1309-2 Komegome, Asahi City, Chiba 289-0516, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Research Institute of Biosciences, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Hideharu
authors.initials: H
authors.lastname: Ochiai
authors.affiliation: Laboratory of Anatomy I, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Nobutsune
authors.initials: N
authors.lastname: Ichihara
authors.affiliation: Laboratory of Physiology II, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Orito
authors.affiliation: Laboratory of Anatomy II, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Arishima
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D002256:Carbonic Anhydrases / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007668:Kidney / Q000201:enzymology*; D007674:Kidney Diseases / Q000201:enzymology / Q000209:etiology / Q000652:urine / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000201:enzymology* / Q000209:etiology / Q000652:urine*
chemical_list: D015415:Biomarkers; D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI
keywords: Biomarker; Carbonic anhydrase-VI; Kidney; Pig; Urine