title: Protective effect of myokine IL-15 against H2O2-mediated oxidative stress in skeletal muscle cells.
abstract: The production of reactive oxygen species (ROS) during oxidative stress may cause cellular injury. Interleukin-15 (IL-15) is one of the skeletal muscle secreted myokines, and there is no information that reported its anti-oxidative capability in skeletal muscle. The aim of this study therefore is to investigate the protective effects of myokine IL-15 against H2O2-mediated oxidative stress in C2C12 myoblasts. The results showed that IL-15 pre-incubation reduced the intracellular creatine kinase and lactate dehydrogenase activities, decreased the ROS overload, and protect the mitochondrial network via up-regulated mRNA expression levels of IL-15 and uncoupling protein 3. It also down-regulated the levels of IL-6 and p21 of the myoblasts compared to the cells treated only with H2O2. Meanwhile, apurinic/aprimidinic endonuclease 1 expression and the Akt signaling pathway were stimulated. These effects could contribute to the resumption of cell viability and act as protective mechanism. In conclusion, myokine IL-15 could be a novel endogenous regulator to control intracellular ROS production and attenuate oxidative stress in skeletal muscle cells.
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China, lifengna@isa.ac.cn.
authors.forename: Fengna
authors.initials: F
authors.lastname: Li
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.forename: Yulong
authors.initials: Y
authors.lastname: Tang
authors.forename: Binbin
authors.initials: B
authors.lastname: Lin
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Kong
authors.forename: Oso Abimbola
authors.initials: OA
authors.lastname: Oladele
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D003402:Creatine Kinase / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000009:adverse effects; D019409:Interleukin-15 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology*; D032446:Myoblasts / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D019409:Interleukin-15; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase
references.citation: Cell Death Differ. 2010 Sep;17(9):1420-34
references.pmid: 20203691
references.citation: Free Radic Biol Med. 2011 Sep 15;51(6):1106-15
references.pmid: 21762777
references.citation: Cancer Sci. 2009 Jul;100(7):1275-83
references.pmid: 19432898
references.citation: Immunity. 2012 Jan 27;36(1):68-78
references.pmid: 22206904
references.citation: Endocrinology. 2013 Jan;154(1):232-45
references.pmid: 23161867
references.citation: Med Hypotheses. 2013 Jun;80(6):776-7
references.pmid: 23566659
references.citation: Blood. 2009 Sep 17;114(12):2417-26
references.pmid: 19605850
references.citation: Cell. 2006 Oct 20;127(2):397-408
references.pmid: 17055439
references.citation: Am J Physiol Heart Circ Physiol. 2005 Jul;289(1):H17-9
references.pmid: 15961373
references.citation: Med Sci Sports Exerc. 2010 Feb;42(2):304-13
references.pmid: 20083961
references.citation: Science. 1994 May 13;264(5161):965-8
references.pmid: 8178155
references.citation: J Appl Physiol (1985). 2009 Oct;107(4):1006-14
references.pmid: 19696361
references.citation: Free Radic Biol Med. 2008 Sep 1;45(5):592-601
references.pmid: 18515104
references.citation: Exp Gerontol. 2002 Oct-Nov;37(10-11):1229-36
references.pmid: 12470836
references.citation: Antioxid Redox Signal. 2006 Sep-Oct;8(9-10):1749-56
references.pmid: 16987028
references.citation: Free Radic Biol Med. 2012 Sep 1;53(5):1068-79
references.pmid: 22796148
references.citation: J Leukoc Biol. 2009 Jul;86(1):187-94
references.pmid: 19389797
references.citation: Free Radic Biol Med. 2010 Dec 1;49(11):1646-54
references.pmid: 20801212
references.citation: J Neuroimmunol. 2010 Jun;223(1-2):65-72
references.pmid: 20430449
references.citation: Cell Metab. 2005 Aug;2(2):85-93
references.pmid: 16098826
references.citation: Leuk Res. 2005 Nov;29(11):1237-8
references.pmid: 15946739
references.citation: Cytokine Growth Factor Rev. 2013 Oct;24(5):455-66
references.pmid: 23791986
references.citation: Cell Biol Int. 2011 Nov;35(11):1141-6
references.pmid: 21510842
references.citation: Mol Carcinog. 1999 Jan;24(1):15-24
references.pmid: 10029406
references.citation: DNA Repair (Amst). 2004 Aug-Sep;3(8-9):1109-15
references.pmid: 15279799
references.citation: Oxid Med Cell Longev. 2012;2012:145418
references.pmid: 22666517
references.citation: Exerc Sport Sci Rev. 2005 Jul;33(3):114-9
references.pmid: 16006818
references.citation: Acta Physiol Scand. 2003 Jun;178(2):107-16
references.pmid: 12780384
references.citation: Proc Nutr Soc. 2004 May;63(2):269-73
references.pmid: 15294042
references.citation: Adv Biol Regul. 2012 May;52(2):340-50
references.pmid: 22781747
references.citation: Proc Natl Acad Sci U S A. 1994 May 24;91(11):4935-9
references.pmid: 8197160
references.citation: Biochim Biophys Acta. 2006 Nov;1760(11):1613-7
references.pmid: 17056184
references.citation: J Immunol. 2002 Aug 15;169(4):1753-9
references.pmid: 12165496
references.citation: Am J Respir Cell Mol Biol. 2002 May;26(5):587-93
references.pmid: 11970911
references.citation: Trends Biochem Sci. 2010 May;35(5):298-307
references.pmid: 20006514
references.citation: Mol Syst Biol. 2010;6:347
references.pmid: 20160708
references.citation: Cell Mol Life Sci. 2010 Nov;67(21):3589-608
references.pmid: 20706766
references.citation: Science. 1995 Feb 17;267(5200):1018-21
references.pmid: 7863327
references.citation: Nat Rev Cancer. 2009 Jun;9(6):400-14
references.pmid: 19440234