title: First report of Perkinsus beihaiensis in wild clams Anomalocardia brasiliana (Bivalvia: Veneridae) in Brazil.
abstract: This is the first report of Perkinsus sp. (Bivalvia: Veneridae) infecting wild clams of the species Anomalocardia brasiliana in Brazil. The gill lamellae and rectum of 150 specimens of A. brasiliana collected in the Timonha river estuary (Ceará, Northeastern Brazil) in March 2012 were incubated in Ray's fluid thioglycollate medium (RFTM) for detection of Perkinsus sp. In RFTM, the prevalence of Perkinsus sp. was 14.7% (22/150) and the intensity of infection ranged from very light (1-10 cells across the slide) to light (12-100 cells). The presence of Perkinsus sp. was confirmed by PCR in seven (31.8%) out of 22 RFTM-positive specimens. DNA sequencing confirmed the presence of the genus Perkinsus and the phylogenetic analysis strongly indicated Perkinsus beihaiensis as the species responsible for the infection.
authors.affiliation: Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3507, Meireles, CEP 60165-081 Fortaleza, CE, Brazil. Electronic address: lianapinho@hotmail.com.
authors.forename: Liana
authors.initials: L
authors.lastname: Pinho Ferreira
authors.affiliation: Instituto Federal de Educação Ciência e Tecnologia, Campus Aracati, Rua Teófilo Pinto, 200, Farias Brito, CEP 62000-800 Aracati, CE, Brazil. Electronic address: rachelsabry@ifce.edu.br.
authors.forename: Rachel Costa
authors.initials: RC
authors.lastname: Sabry
authors.affiliation: Universidade Federal da Paraíba, Centro de Ciências Exatas e da Natureza, Departamento de Biologia Molecular, Cidade Universitária - Campus I, CEP 58059-900 João Pessoa, PB, Brazil. Electronic address: mirella_dasilva@hotmail.com.
authors.forename: Patrícia Mirella
authors.initials: PM
authors.lastname: da Silva
authors.affiliation: Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3507, Meireles, CEP 60165-081 Fortaleza, CE, Brazil. Electronic address: cvgesteira@secrel.com.br.
authors.forename: Tereza Cristina Vasconcelos
authors.initials: TC
authors.lastname: Gesteira
authors.affiliation: Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3507, Meireles, CEP 60165-081 Fortaleza, CE, Brazil. Electronic address: li-romao@hotmail.com.
authors.forename: Lidiane
authors.initials: L
authors.lastname: de Souza Romão
authors.affiliation: Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3507, Meireles, CEP 60165-081 Fortaleza, CE, Brazil. Electronic address: marcelapinheiropaz@hotmail.com.
authors.forename: Marcela Pinheiro
authors.initials: MP
authors.lastname: Paz
authors.affiliation: Universidade Federal do Rio Grande, Instituto de Ciências Biológicas, Campus Carreiros, Av. Itália, Km 8, CEP 96.201-900 Rio Grande, RS, Brazil. Electronic address: rubensfeijo2005@yahoo.com.br.
authors.forename: Rubens Galdino
authors.initials: RG
authors.lastname: Feijó
authors.affiliation: Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3507, Meireles, CEP 60165-081 Fortaleza, CE, Brazil. Electronic address: maxfloyd@hotmail.com.
authors.forename: Maximiano Pinheiro Dantas
authors.initials: MP
authors.lastname: Neto
authors.affiliation: Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. da Abolição, 3507, Meireles, CEP 60165-081 Fortaleza, CE, Brazil. Electronic address: maggioni@ufc.br.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Maggioni
mesh_terms: D056893:Alveolata / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000469:parasitology*; D001938:Brazil; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D005880:Gills / Q000469:parasitology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D012335:RNA, Ribosomal; D004247:DNA
keywords: Bivalves; Clams; Perkinsus beihaiensis; Protozoa