title: Validated spectrofluorimetric method for determination of selected aminoglycosides.
abstract: New, sensitive, and selective spectrofluorimetric method was developed for determination of three aminoglycoside drugs in different dosage forms, namely; neomycin sulfate (NEO), tobramycin (TOB) and kanamycin sulfate (KAN). The method is based on Hantzsch condensation reaction between the primary amino group of aminoglycosides with acetylacetone and formaldehyde in pH 2.7 yielding highly yellow fluorescent derivatives measured emission (471 nm) and excitation (410 nm) wavelengths. The fluorescence intensity was directly proportional to the concentration over the range 10-60, 40-100 and 5-50 ng/mL for NEO, TOB and KAN respectively. The proposed method was applied successfully for determination of these drugs in their pharmaceutical dosage forms.
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Omar
authors.affiliation: Pharmaceutical Analysis Department, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt. Electronic address: hmaahmed@yahoo.co.uk.
authors.forename: Hytham M
authors.initials: HM
authors.lastname: Ahmed
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Hammad
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
authors.forename: Sayed M
authors.initials: SM
authors.lastname: Derayea
mesh_terms: D000617:Aminoglycosides / Q000032:analysis* / Q000737:chemistry; D004304:Dosage Forms; D007202:Indicators and Reagents; D007612:Kanamycin / Q000032:analysis / Q000737:chemistry; D057230:Limit of Detection; D009355:Neomycin / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014031:Tobramycin / Q000032:analysis / Q000737:chemistry
chemical_list: D000617:Aminoglycosides; D004304:Dosage Forms; D007202:Indicators and Reagents; D007612:Kanamycin; D009355:Neomycin; D014031:Tobramycin
keywords: Aminoglycosides; Hantzsch reaction; Kanamycin; Neomycin; Spectrofluorimetry; Tobramycin