title: [Fingerprint research on immunosuppressive fraction of Rehmanniae Radix by HPLC].
abstract: OBJECTIVE
  To study the HPLC fingerprint of immunosuppressive fraction of Rehmanniae Radix, the genuine medicinal materials in Henan province.
  
  
  METHODS
  By HPLC method,Dikma Platisil-C18 column (250 mm x 4.6 mm, 5 microm) was used with acetonitrile-0.1% phosphoric acid as mobile phase by gradient elution. The detection wavelength was 210 nm, flow rate was 1.0 mL/min and column temperature was set at 30 degrees C.
  
  
  RESULTS
  Compared the 10 batches sample of the fingerprint similarity, 18 common peaks were marked and five peaks were identified. The similarities were more than 0.9.
  
  
  CONCLUSIONS
  HPLC fingerprint of immunosuppressive fraction of Rehmanniae Radix is established, which lays the foundation for further pharmacodynamics research on Rehmanniae Radix.
authors.forename: Xiao-ke
authors.initials: XK
authors.lastname: Zheng
authors.forename: Yu-guang
authors.initials: YG
authors.lastname: Jia
authors.forename: Zhi-yi
authors.initials: ZY
authors.lastname: Feng
authors.forename: Shen
authors.initials: S
authors.lastname: Wang
authors.forename: Ming-hui
authors.initials: MH
authors.lastname: Zhang
authors.forename: Wei-sheng
authors.initials: WS
authors.lastname: Feng
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D007166:Immunosuppressive Agents / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D011786:Quality Control; D032267:Rehmannia / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D007166:Immunosuppressive Agents; D000431:Ethanol