title: A novel crosslinked hyaluronic acid nanogel for drug delivery.
abstract: An amphiphilic hyaluronic acid conjugate is successfully developed based on grafting a thiolated hydrophobic molecule to the polysaccharide backbone. The engineered conjugate is capable of assembling into nanostructures once dispersed in water, with average diameter of 80.2 ± 0.4 nm (n = 5), stable up to 6 months. The thiolated HyA conjugate is reticulated by dissulfide bond with a homofunctional crosslinker-1,4-Bis(3-[2-pyridyldithio]propionamido)butane (DPDPB). The drug loading efficiency of the reticulated and non-reticulated nanogel is accessed with two hydrophobic drugs, curcumin and simvastatin. Results suggest that crosslinked nanogel exhibit higher stability upon dilution and drug loading efficiency and proves to be a redox sensitive material. The nanogels hold great potential as stealth carriers of lipophilic drugs.
authors.affiliation: Institute for Biotechnology and Bioengineering (IBB), Centre for Biological Engineering, Campus de Gualtar University of Minho, Braga, Portugal.
authors.forename: Sílvia Santos
authors.initials: SS
authors.lastname: Pedrosa
authors.forename: Catarina
authors.initials: C
authors.lastname: Gonçalves
authors.forename: Laurent
authors.initials: L
authors.lastname: David
authors.forename: Miguel
authors.initials: M
authors.lastname: Gama
mesh_terms: D002073:Butanes / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D020285:Cryoelectron Microscopy; D003474:Curcumin / Q000494:pharmacology*; D016503:Drug Delivery Systems; D006820:Hyaluronic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D057446:Hydrodynamics; D008027:Light; D008970:Molecular Weight; D000080385:Nanogels; D010084:Oxidation-Reduction; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D012542:Scattering, Radiation; D053838:Scattering, Small Angle; D019821:Simvastatin / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014961:X-Ray Diffraction
chemical_list: D002073:Butanes; D003102:Colloids; D003432:Cross-Linking Reagents; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; C046888:butane; D011094:Polyethyleneimine; D006820:Hyaluronic Acid; D019821:Simvastatin; D003474:Curcumin