title: Polyethyleneglycol diacrylate microspheres: a novel carrier for laccase immobilisation.
abstract: Laccase was immobilised on polyethyleneglycol diacrylate (PEGDA) microspheres. The optimal preparation conditions of PEGDA microspheres were as follows: 3.0% (w/v) 2,2-azobisisobutyro-nitrite (AIBN), 4.0-5.0% (w/v) polyvinylpyrrolidone (PVP), 5.0-8.0% (w/v) glucose and 4.0% (w/v) PEGDA in glucose solution. The volume ratio of PEGDA solution, glucose/PVP solution and AIBN solution was 25: 100: 1. Microspheres obtained exhibited good characteristics with small sizes (1-4 µm). The immobilised laccase showed a higher stability in a wide pH range. Thermal stability and storage stability of immobilised laccase were enhanced. The activity of immobilised laccase was 45.0% after six cycles uses. Only 62.7% of the activity remained for free laccase while there was a 60.4% increased for immobilised laccase with storage at 4 °C for 25 d. The Km value of laccase increased from 21.9 to 114.0 µmol/l after immobilisation.
authors.affiliation: College of Life Sciences, Northeast Forestry University , Harbin , P.R. China and.
authors.forename: Xiao Yan
authors.initials: XY
authors.lastname: Li
authors.forename: Shu Yu
authors.initials: SY
authors.lastname: Yu
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
mesh_terms: D004337:Drug Carriers / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D042845:Laccase / Q000737:chemistry*; D008863:Microspheres; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D004800:Enzymes, Immobilized; C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols; D042845:Laccase
keywords: Enzyme activity; immobilisation; laccase; polyethyleneglycol diacrylate microspheres