title: γEpithelial Na(+) Channel (γENaC) and the Acid-Sensing Ion Channel 1 (ASIC1) expression in the urothelium of patients with neurogenic detrusor overactivity.
abstract: OBJECTIVE
  To investigate the expression of two types of cation channels, γEpithelial Na(+) Channel (γENaC) and the Acid-Sensing Ion Channel 1 (ASIC1), in the urothelium of controls and in patients affected by neurogenic detrusor overactivity (NDO). In parallel, urodynamic parameters were collected and correlated to the immunohistochemical results.
  
  
  METHODS
  Four controls and 12 patients with a clinical diagnosis of NDO and suprasacral spinal cord lesion underwent urodynamic measurements and cystoscopy. Cold-cup biopsies were frozen and processed for immunohistochemistry and Western Blot. Spearman's correlation coefficient between morphological and urodynamic data was applied. One-way anova followed by Newman-Keuls multiple comparison post hoc test was applied for Western Blot results.
  
  
  RESULTS
  In the controls, γENaC and ASIC1 were expressed in the urothelium with differences in their cell distribution and intensity. In patients with NDO, both markers showed consistent changes either in cell distribution and labelling intensity compared with the controls. A significant correlation between a higher intensity of γENaC expression in the urothelium of patients with NDO and lower values of bladder compliance was detected.
  
  
  CONCLUSIONS
  The present findings show important changes in the expression of γENaC and ASIC1 in NDO human urothelium. Notably, while the changes in γENaC might impair the mechanosensory function of the urothelium, the increase of ASIC1 might represent an attempt to compensate for the excess in local sensitivity.
authors.affiliation: Department of Experimental and Clinical Medicine, Histology and Embryology Research Unit, University of Florence, Florence, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Traini
authors.affiliation: Department of Neuro-Urology, Careggi University Hospital, Florence, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Del Popolo
authors.affiliation: Division of Oncology, Unit of Urology, URI, IRCCS Ospedale San Raffaele, Università Vita-Salute San Raffaele, Milan, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Lazzeri
authors.affiliation: Department of Experimental and Clinical Medicine, Histology and Embryology Research Unit, University of Florence, Florence, Italy.
authors.forename: Katia
authors.initials: K
authors.lastname: Mazzaferro
authors.affiliation: Department of Neuro-Urology, Careggi University Hospital, Florence, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Nelli
authors.affiliation: Department of Experimental and Clinical Medicine, Histology and Embryology Research Unit, University of Florence, Florence, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Calosi
authors.affiliation: Department of Experimental and Clinical Medicine, Histology and Embryology Research Unit, University of Florence, Florence, Italy.
authors.forename: Maria Giuliana
authors.initials: MG
authors.lastname: Vannucchi
mesh_terms: D062565:Acid Sensing Ion Channels / Q000378:metabolism*; D003558:Cystoscopy / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D001743:Urinary Bladder / Q000473:pathology*; D001750:Urinary Bladder, Neurogenic / Q000378:metabolism* / Q000503:physiopathology; D053201:Urinary Bladder, Overactive / Q000378:metabolism* / Q000503:physiopathology; D019459:Urothelium / Q000378:metabolism* / Q000473:pathology
chemical_list: C568266:ASIC1 protein, human; D062565:Acid Sensing Ion Channels
keywords: cystoscopy; degenerin family; immunohistochemistry; spinal cord lesion; urodynamic parameters; western blot