title: CD8α¯ DC is the major DC subset which mediates inhibition of allergic responses by Schistosoma infection.
abstract: Our and others' previous studies have shown that Schistosoma japonicum (SJ) infection can inhibit allergic reactions. We recently reported that DCs played an important role in SJ infection-mediated inhibition of allergy, which was associated with enhanced IL-10 and T regulatory cell responses. Here, we further compared the role of CD8α(+) DC and CD8α(-) DC subsets for the inhibitory effect. We sorted CD8α(+) DC (SJCD8α(+) DC) and CD8α(-) DC (SJCD8α(-) DC) from SJ-infected mice and tested their ability to modulate allergic responses in vivo. The data showed that the adoptive transfer of SJCD8α(-) DC was much more efficient than SJCD8α(+) DC for the suppression of allergic airway eosinophilia, mucus overproduction, antigen-specific IgE responses, and Th2 cytokines (IL-4 and IL-5). More importantly, we found that the transfer of SJCD8α(-) DC, but not SJCD8α(+) DC, significantly increased IL-10 and TGF-β production following OVA exposure. As control, the transfer of DC subsets from naïve mice had no significant effect on allergic inflammation. In addition, SJCD8α-DC expressed significantly higher IL-10 but lower IL-12, CD80 and CD86 than SJCD8α(+) DC, fitting a tolerogenic phenotype. The results suggest that CD8α(-) DC is the predominant DC subset which is involved in the parasitic infection-mediated inhibition of allergic inflammation and possibly through enhancing immunomodulatory cytokine (IL-10 and TGF-β) production.
authors.affiliation: Laboratory of Cellular and Molecular Immunology, Department of Parasitology, Faculty of Basic Medicine Sciences, Tianjin Medical University, Tianjin, China.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Liu
authors.forename: P
authors.initials: P
authors.lastname: Lu
authors.forename: L-Z
authors.initials: LZ
authors.lastname: Hu
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Shen
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Zhu
authors.forename: J-L
authors.initials: JL
authors.lastname: Ren
authors.forename: W-H
authors.initials: WH
authors.lastname: Ji
authors.forename: X-Z
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Z-Q
authors.initials: ZQ
authors.lastname: Wu
authors.forename: X-Z
authors.initials: XZ
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: L-Y
authors.initials: LY
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: P-M
authors.initials: PM
authors.lastname: Liu
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D039481:CD11b Antigen; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D006967:Hypersensitivity / Q000276:immunology* / Q000517:prevention & control; D016753:Interleukin-10 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D011014:Pneumonia / Q000139:chemically induced / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D039481:CD11b Antigen; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D010047:Ovalbumin
keywords: DC subsets; Schistosoma japonicum; allergy; hygiene hypothesis