title: Novel molecular alterations in the ORF 2 capsid gene of hepatitis E virus in patients with acute liver failure in North India.
abstract: Hepatitis E virus (HEV) is evolving as a major global threat to public health, including in developed countries. We partially sequenced the ORF 2 capsid protein genes of HEV genomes from patients with acute liver failure, including pregnant women in the northern part of India. Five unique synonymous substitutions and one non-synonymous substitution, along with a novel mutation, P259S, in the capsid gene, were identified that might be associated with the poor outcome in the patients. Phylogenetic analysis revealed that the isolates belonged to genotype 1 with subtype 1a. The significance of these findings for disease pathogenicity needs to be investigated further.
authors.affiliation: PCR Hepatitis Laboratory, Department of Medicine, Maulana Azad Medical College and Associated Lok Nayak Jai Prakash Hospital, University of Delhi, New Delhi, India.
authors.forename: Jayanta
authors.initials: J
authors.lastname: Borkakoti
authors.forename: Giasuddin
authors.initials: G
authors.lastname: Ahmed
authors.forename: Syed Akhtar
authors.initials: SA
authors.lastname: Hussain
authors.forename: Arvind
authors.initials: A
authors.lastname: Rai
authors.forename: Premashis
authors.initials: P
authors.lastname: Kar
mesh_terms: D016000:Cluster Analysis; D016751:Hepatitis E / Q000150:complications* / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007194:India; D017114:Liver Failure, Acute / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017354:Point Mutation; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: C400971:ORF2 protein, Hepatitis E virus; D012367:RNA, Viral; D014764:Viral Proteins