title: A novel finding of anoctamin 5 expression in the rodent gastrointestinal tract.
abstract: Anoctamin 5 (Ano5) belongs to the anoctamin gene family and acts as a calcium-activated chloride channel (CaCC). A mutation in the Ano5 gene causes limb-girdle muscular dystrophy (LGMD) type 2L, the third most common LGMD in Northern and Central Europe. Defective sarcolemmal membrane repair has been reported in patients carrying this Ano5 mutant. It has also been noted that LGMD patients often suffer from nonspecific pharyngoesophageal motility disorders. One study reported that 8/19 patients carrying Ano5 nutations suffered from dysphagia, including the feeling that solid food items become lodged in the upper portion of the esophagus. Ano5 is widely distributed in bone, skeletal muscle, cardiac muscle, brain, heart, kidney and lung tissue, but no report has examined its expression in the gastrointestinal (GI) tract. In the present study, we investigated the distribution of Ano5 in the GI tracts of mice via reverse transcription-polymerase chain reaction (RT-PCR), Western blot and immunofluorescence analyses. The results indicated that Ano5 mRNA and protein are widely expressed in the esophagus, the stomach, the duodenum, the colon and the rectum but that Ano5 immunoreactivity was only detected in the mucosal layer, except for the muscular layer of the upper esophagus, which consists of skeletal muscle. In conclusion, our present results demonstrate for the first time the expression of Ano5 in the GI epithelium and in skeletal muscle in the esophagus. This novel finding facilitates clinical differential diagnosis and treatment. However, further investigation of the role of Ano5 in GI function is required.
authors.affiliation: Key Laboratory for Medical Tissue Regeneration of Henan Province, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, PR China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Song
authors.affiliation: Key Laboratory for Medical Tissue Regeneration of Henan Province, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, PR China. Electronic address: opopiuy@163.com.
authors.forename: Yue-Min
authors.initials: YM
authors.lastname: Tian
authors.affiliation: Key Laboratory for Medical Tissue Regeneration of Henan Province, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, PR China.
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Medical Tissue Regeneration of Henan Province, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Medical Tissue Regeneration of Henan Province, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lian
authors.affiliation: Key Laboratory for Medical Tissue Regeneration of Henan Province, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, PR China; Department of Physiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China. Electronic address: zhu_jx@ccmu.edu.cn.
authors.forename: Jin-Xia
authors.initials: JX
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000075368:Anoctamins; D015153:Blotting, Western; D018118:Chloride Channels / Q000235:genetics* / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D049288:Muscular Dystrophies, Limb-Girdle / Q000235:genetics; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012508:Sarcolemma / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: C547813:ANO5 protein, human; C000592508:ANO5 protein, mouse; D000075368:Anoctamins; D018118:Chloride Channels; C502024:GDD1 protein, mouse; D008565:Membrane Proteins; D012333:RNA, Messenger
keywords: Ano5; Colon; Duodenum; Esophagus; Gastrointestinal tract; Stomach