title: Performance of two new automated assays for measuring von Willebrand activity: HemosIL AcuStar and Innovance.
abstract: The ristocetin cofactor activity assay (VWF:RCo) is the reference method for assessing von Willebrand factor (VWF) activity but remains difficult to perform, and the coefficient of variation of the method is high (about 20-30%). This study evaluated and compared the performance for measuring the VWF activity of two newly commercialised assays [VWF:Ac Innovance (VWF:Ac) and VWF:RCo Acustar (VWF:RCo Acu)] with the reference VWF:RCo aggregation in 123 pathological plasma samples. The correlation and concordance between both new tests (VWF:RCo-Acu and VWF:Ac) and the reference VWF:RCo were good. The results of the VWF activity to VWF antigen ratio were also comparable whatever the method for the classification of VWF deficiency in all patients. Our results showed that both new tests could replace the "gold standard" VWF:RCo in aggregometry with several benefits: they are fully automated, easier and faster to perform, better adapted to emergency situations if necessary.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: de Maistre
authors.forename: Fabienne
authors.initials: F
authors.lastname: Volot
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mourey
authors.forename: Ludwig S
authors.initials: LS
authors.lastname: Aho
authors.forename: Catherine
authors.initials: C
authors.lastname: Ternisien
authors.forename: Marie-Elisabeth
authors.initials: ME
authors.lastname: Briquel
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Bertrand
authors.forename: Brigitte
authors.initials: B
authors.lastname: Tardy
authors.forename: Birgit
authors.initials: B
authors.lastname: Frotscher
authors.forename: Philippe
authors.initials: P
authors.lastname: Nguyen
authors.forename: Laure
authors.initials: L
authors.lastname: Dumont
authors.forename: David
authors.initials: D
authors.lastname: Vandroux
authors.forename: Nathalie
authors.initials: N
authors.lastname: Hézard
authors.affiliation: Dr. Marc Trossaërt, Centre Régional de Traitement de l'Hémophilie, 1 Place Alexis RICORDEAU, Centre Hospitalier Universitaire, 44093 Nantes Cedex 1, France, Tel.: +33 2 40 08 74 68, Fax: +33 2 40 08 42 59, E-mail: marc.trossaert@chu-nantes.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Trossaërt
mesh_terms: D001331:Automation; D001777:Blood Coagulation; D001780:Blood Coagulation Tests / Q000379:methods*; D002138:Calibration; D016022:Case-Control Studies; D003094:Collagen / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010974:Platelet Aggregation; D011446:Prospective Studies; D012016:Reference Values; D015203:Reproducibility of Results; D012310:Ristocetin / Q000097:blood; D012680:Sensitivity and Specificity; D014842:von Willebrand Diseases / Q000097:blood* / Q000175:diagnosis; D014841:von Willebrand Factor / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism
chemical_list: D014841:von Willebrand Factor; D012310:Ristocetin; D003094:Collagen
keywords: Willebrand activity assay; aggregometry; ristocetin cofactor activity; von Willebrand disease