title: Mapping of quantitative adult plant field resistance to leaf rust and stripe rust in two European winter wheat populations reveals co-location of three QTL conferring resistance to both rust pathogens.
abstract: We detected several, most likely novel QTL for adult plant resistance to rusts. Notably three QTL improved resistance to leaf rust and stripe rust simultaneously indicating broad spectrum resistance QTL. The rusts of wheat (Puccinia spp.) are destructive fungal wheat diseases. The deployment of resistant cultivars plays a central role in integrated rust disease management. Durability of resistance would be preferred, but is difficult to analyse. The Austrian winter wheat cultivar Capo was released in the 1989 and grown on a large acreage during more than two decades and maintained a good level of quantitative leaf rust and stripe rust resistance. Two bi-parental mapping populations: Capo × Arina and Capo × Furore were tested in multiple environments for severity of leaf rust and stripe rust at the adult plant stage in replicated field experiments. Quantitative trait loci associated with leaf rust and stripe rust severity were mapped using DArT and SSR markers. Five QTL were detected in multiple environments associated with resistance to leaf rust designated as QLr.ifa-2AL, QLr.ifa-2BL, QLr.ifa-2BS, QLr.ifa-3BS, and QLr.ifa-5BL, and five for resistance to stripe rust QYr.ifa-2AL, QYr.ifa-2BL, QYr.ifa-3AS, QYr.ifa-3BS, and QYr.ifa-5A. For all QTL apart from two (QYr.ifa-3AS, QLr.ifa-5BL) Capo contributed the resistance improving allele. The leaf rust and stripe rust resistance QTL on 2AL, 2BL and 3BS mapped to the same chromosome positions, indicating either closely linked genes or pleiotropic gene action. These three multiple disease resistance QTL (QLr.ifa-2AL/QYr.ifa-2AL, QLr.ifa.2BL/QYr.ifa-2BL, QLr.ifa-3BS/QYr.ifa.3BS) potentially contribute novel resistance sources for stripe rust and leaf rust. The long-lasting resistance of Capo apparently rests upon a combination of several genes. The described germplasm, QTL and markers are applicable for simultaneous resistance improvement against leaf rust and stripe rust.
authors.affiliation: Department for Agrobiotechnology Tulln, BOKU-University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Str. 20, Tulln, 3430, Austria.
authors.forename: Maria
authors.initials: M
authors.lastname: Buerstmayr
authors.forename: Lydia
authors.initials: L
authors.lastname: Matiasch
authors.forename: Fabio
authors.initials: F
authors.lastname: Mascher
authors.forename: Gyula
authors.initials: G
authors.lastname: Vida
authors.forename: Marianna
authors.initials: M
authors.lastname: Ittu
authors.forename: Olivier
authors.initials: O
authors.lastname: Robert
authors.forename: Sarah
authors.initials: S
authors.lastname: Holdgate
authors.forename: Kerstin
authors.initials: K
authors.lastname: Flath
authors.forename: Anton
authors.initials: A
authors.lastname: Neumayer
authors.forename: Hermann
authors.initials: H
authors.lastname: Buerstmayr
mesh_terms: D001487:Basidiomycota / Q000472:pathogenicity*; D001947:Breeding; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D060467:Disease Resistance / Q000235:genetics*; D008040:Genetic Linkage; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics*; D040641:Quantitative Trait Loci; D014908:Triticum / Q000235:genetics*
references.citation: Theor Appl Genet. 2007 May;114(7):1277-87
references.pmid: 17318493
references.citation: Genetics. 2001 Sep;159(1):371-87
references.pmid: 11560912
references.citation: Theor Appl Genet. 2009 Sep;119(5):889-98
references.pmid: 19578829
references.citation: Theor Appl Genet. 1999 Aug;99(3-4):554-60
references.pmid: 22665189
references.citation: Mol Genet Genomics. 2009 Feb;281(2):181-91
references.pmid: 19020902
references.citation: Theor Appl Genet. 2007 Oct;115(6):877-84
references.pmid: 17646964
references.citation: Theor Appl Genet. 2012 Dec;125(8):1619-38
references.pmid: 22872151
references.citation: Heredity (Edinb). 1992 Oct;69(4):315-24
references.pmid: 16718932
references.citation: Genome. 2005 Feb;48(1):97-107
references.pmid: 15729401
references.citation: Theor Appl Genet. 2008 Jun;117(1):103-15
references.pmid: 18437346
references.citation: Theor Appl Genet. 2009 Jun;119(1):13-21
references.pmid: 19330313
references.citation: Phytopathology. 2003 Jul;93(7):784-9
references.pmid: 18943158
references.citation: Theor Appl Genet. 2008 May;116(8):1155-66
references.pmid: 18347772
references.citation: Phytopathology. 2009 Aug;99(8):968-73
references.pmid: 19594316
references.citation: Theor Appl Genet. 2008 Nov;117(7):1069-75
references.pmid: 18651124
references.citation: Proc Natl Acad Sci U S A. 1984 Dec;81(24):8014-8
references.pmid: 6096873
references.citation: Theor Appl Genet. 2009 Jul;119(2):263-9
references.pmid: 19396420
references.citation: Theor Appl Genet. 2004 Oct;109(6):1105-14
references.pmid: 15490101
references.citation: Theor Appl Genet. 2005 Feb;110(3):550-60
references.pmid: 15655666
references.citation: Theor Appl Genet. 2006 Nov;113(8):1409-20
references.pmid: 17033786
references.citation: Mol Plant Pathol. 2008 Sep;9(5):563-75
references.pmid: 19018988
references.citation: Theor Appl Genet. 2008 Aug;117(3):307-12
references.pmid: 18542911
references.citation: Theor Appl Genet. 2013 Oct;126(10):2427-49
references.pmid: 23955314
references.citation: Theor Appl Genet. 2012 May;124(7):1283-94
references.pmid: 22274764
references.citation: Theor Appl Genet. 2004 Feb;108(3):477-84
references.pmid: 14523520
references.citation: Theor Appl Genet. 2012 Sep;125(5):847-57
references.pmid: 22562146
references.citation: Bioinformatics. 2003 May 1;19(7):889-90
references.pmid: 12724300
references.citation: Phytopathology. 2003 Feb;93(2):153-9
references.pmid: 18943129
references.citation: Genome. 2006 Aug;49(8):977-90
references.pmid: 17036073
references.citation: Theor Appl Genet. 2005 Jan;110(2):244-51
references.pmid: 15672257
references.citation: Theor Appl Genet. 2011 Aug;123(4):615-23
references.pmid: 21573954
references.citation: Phytopathology. 2003 Jul;93(7):881-90
references.pmid: 18943170
references.citation: Phytopathology. 2009 Jan;99(1):89-94
references.pmid: 19055439
references.citation: Theor Appl Genet. 2004 Feb;108(3):567-75
references.pmid: 14523516
references.citation: J Appl Genet. 2012 Feb;53(1):19-25
references.pmid: 22033869
references.citation: Theor Appl Genet. 2012 May;124(7):1305-14
references.pmid: 22278178
references.citation: Theor Appl Genet. 2013 Jul;126(7):1721-32
references.pmid: 23558982
references.citation: Science. 2009 Mar 6;323(5919):1360-3
references.pmid: 19229000
references.citation: Theor Appl Genet. 2012 Sep;125(5):955-65
references.pmid: 22610360
references.citation: Theor Appl Genet. 2005 Aug;111(4):731-5
references.pmid: 15965649
references.citation: Genetics. 1998 Aug;149(4):2007-23
references.pmid: 9691054
references.citation: Phytopathology. 1998 Sep;88(9):890-4
references.pmid: 18944865
references.citation: Theor Appl Genet. 2008 May;116(7):1027-34
references.pmid: 18335201
references.citation: Bioinformatics. 2005 Apr 15;21(8):1703-4
references.pmid: 15598829
references.citation: Theor Appl Genet. 2008 Nov;117(8):1225-40
references.pmid: 18712342
references.citation: Theor Appl Genet. 2011 Jun;123(1):143-57
references.pmid: 21455722
references.citation: Genome. 1997 Apr;40(2):253-60
references.pmid: 18464825
references.citation: Euphytica. 2011 May 1;179(1):69-79
references.pmid: 26900170
references.citation: Theor Appl Genet. 2011 Dec;123(8):1401-11
references.pmid: 21830107
references.citation: Genetics. 2007 Nov;177(3):1889-913
references.pmid: 17947425
references.citation: Theor Appl Genet. 2006 Feb;112(3):492-9
references.pmid: 16311724
references.citation: Mol Ecol. 2008 Sep;17(17):3818-26
references.pmid: 18673440
references.citation: J Hered. 2002 Jan-Feb;93(1):77-8
references.pmid: 12011185