title: Transformation of membrane nanosurface of red blood cells under hemin action.
abstract: Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane ("grains") with typical sizes of 120-200 nm was experimentally shown. The process of formation of "grains" was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.
authors.affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.
authors.forename: Elena
authors.initials: E
authors.lastname: Kozlova
authors.affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.
authors.forename: Alexander
authors.initials: A
authors.lastname: Chernysh
authors.affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.
authors.forename: Victor
authors.initials: V
authors.lastname: Moroz
authors.affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.
authors.forename: Olga
authors.initials: O
authors.lastname: Gudkova
authors.affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.
authors.forename: Victoria
authors.initials: V
authors.lastname: Sergunova
authors.affiliation: V.A. Negovsky Scientific Research Institute of General Reanimatology RAS, Moscow, Russian Federation.
authors.forename: Artem
authors.initials: A
authors.lastname: Kuzovlev
mesh_terms: D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006427:Hemin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry
chemical_list: D006427:Hemin
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