title: Molecular epidemiological characterization of Staphylococcus aureus isolates originating from food poisoning outbreaks that occurred in Tokyo, Japan.
abstract: Staphylococcal food poisoning (SFP), one of the commonest food-borne diseases, results from the ingestion of one or more staphylococcal enterotoxins (SEs) produced in foods by Staphylococcus aureus. In the present study, 203 S. aureus strains originating from 83 outbreaks that had occurred in Tokyo were examined for their coagulase type and genotype of SEs to analyze their molecular epidemiological characteristics. The representative subsets of the 83 S. aureus isolates were analyzed by multilocus sequence typing (MLST) and S. aureus pathogenicity island (SaPI) scanning. The isolates were integrated into eight specific clonal complexes (CC) s; CC81, CC8, CC6, CC5, CC508, CC59, CC20 and CC30. The profiles of the coagulase type, SE/SEl genotype and the suspected type of enterotoxin-encoding mobile genetic element (MGE) indicated a correlation with each CC. SaPI scanning showed fixed regularity between the distributions of genomic islands, including SaPIs, and the phylogenetic lineage based on MLST. These results indicate that the S. aureus isolates, which classified into eight CCs, have distinguishable properties concerning specific coagulase type, enterotoxin genotype and MGE type. Strains of S. aureus harboring these particular elements possess the potential to cause SFP.
authors.affiliation: Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka City, Iwate, 020-8550; United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu City, Gifu, 501-1193; Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3-24-1 Hyakunin-cho, Shinjuku-ku, Tokyo, 169-0073.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Omoe
authors.forename: Dong-Liang
authors.initials: DL
authors.lastname: Hu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato'o
authors.forename: Hisaya K
authors.initials: HK
authors.lastname: Ono
authors.forename: Chie
authors.initials: C
authors.lastname: Monma
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Arai
authors.forename: Noriko
authors.initials: N
authors.lastname: Konishi
authors.forename: Rei
authors.initials: R
authors.lastname: Kato
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hirai
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakama
authors.forename: Akemi
authors.initials: A
authors.lastname: Kai
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamata
mesh_terms: D004196:Disease Outbreaks; D005517:Foodborne Diseases / Q000453:epidemiology / Q000382:microbiology*; D005838:Genotype; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014041:Tokyo / Q000453:epidemiology
keywords: Staphylococcus aureus pathogenicity island scanning; clonal complex; coagulase type; enterotoxin genotype