title: The Mycoplasma hyopneumoniae recombinant heat shock protein P42 induces an immune response in pigs under field conditions.
abstract: Enzootic pneumonia (EP), resulting from Mycoplasma hyopneumoniae infection is one of the most prevalent diseases in pigs and is a major cause of economic losses to the swine industry worldwide. EP is often controlled by vaccination with inactivated, adjuvanted whole-cell bacterin. However, these bacterins provide only partial protection and do not prevent M. hyopneumoniae colonization. Attempts to develop vaccines that are more efficient have made use of the recombinant DNA technology. The objective of this study was to assess the potential of recombinant M. hyopneumoniae heat shock protein P42 in vaccine preparations against EP, using piglets housed under field conditions in a M. hyopneumoniae-positive farm. The cellular and humoral immune responses were elicited after a single intramuscular inoculation of rP42 in an oil-based adjuvant, or in conjunction with whole-cell vaccine preparation. The production of INF-γ and IL-10 cytokines was quantified in the supernatant of the cultured mononuclear cells. The rP42 emulsified in oil-based adjuvant was able to trigger a strong humoral immune response. Further, it induced a cellular immune response, accompanied by the production of antibodies that reacted with the native M. hyopneumoniae protein. The rP42 mediated induction of cellular and humoral immune response in the host suggests that rP42 emulsified in an oil-based adjuvant holds promise as an effective recombinant subunit vaccine against EP.
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas (UFPel), Campus Capão do Leão, 96010-900 Pelotas, RS, Brazil. Electronic address: sergiojorgevet@hotmail.com.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Jorge
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas (UFPel), Campus Capão do Leão, 96010-900 Pelotas, RS, Brazil.
authors.forename: Natasha Rodrigues
authors.initials: NR
authors.lastname: de Oliveira
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas (UFPel), Campus Capão do Leão, 96010-900 Pelotas, RS, Brazil.
authors.forename: Silvana Beutinger
authors.initials: SB
authors.lastname: Marchioro
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas (UFPel), Campus Capão do Leão, 96010-900 Pelotas, RS, Brazil.
authors.forename: Andressa
authors.initials: A
authors.lastname: Fisch
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas (UFPel), Campus Capão do Leão, 96010-900 Pelotas, RS, Brazil.
authors.forename: Charles Klazer
authors.initials: CK
authors.lastname: Gomes
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas (UFPel), Campus Capão do Leão, 96010-900 Pelotas, RS, Brazil.
authors.forename: Cláudia Pinho
authors.initials: CP
authors.lastname: Hartleben
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas (UFPel), Campus Capão do Leão, 96010-900 Pelotas, RS, Brazil.
authors.forename: Fabricio Rochedo
authors.initials: FR
authors.lastname: Conceição
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas (UFPel), Campus Capão do Leão, 96010-900 Pelotas, RS, Brazil.
authors.forename: Odir Antonio
authors.initials: OA
authors.lastname: Dellagostin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D056724:Immunity, Humoral; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D045705:Mycoplasma hyopneumoniae / Q000235:genetics / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D013552:Swine / Q000276:immunology / Q000382:microbiology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D016207:Cytokines; D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: Enzootic pneumonia; Heat shock protein; Subunit vaccine