title: Easyworm: an open-source software tool to determine the mechanical properties of worm-like chains.
abstract: BACKGROUND
  A growing spectrum of applications for natural and synthetic polymers, whether in industry or for biomedical research, demands for fast and universally applicable tools to determine the mechanical properties of very diverse polymers. To date, determining these properties is the privilege of a limited circle of biophysicists and engineers with appropriate technical skills.
  
  
  RESULTS
  Easyworm is a user-friendly software suite coded in MATLAB that simplifies the image analysis of individual polymeric chains and the extraction of the mechanical properties of these chains. Easyworm contains a comprehensive set of tools that, amongst others, allow the persistence length of single chains and the Young's modulus of elasticity to be calculated in multiple ways from images of polymers obtained by a variety of techniques (e.g. atomic force microscopy, electron, contrast-phase, or epifluorescence microscopy).
  
  
  CONCLUSIONS
  Easyworm thus provides a simple and efficient tool for specialists and non-specialists alike to solve a common problem in (bio)polymer science. Stand-alone executables and shell scripts are provided along with source code for further development.
authors.affiliation: Centre for High-Throughput Biology, University of British Colombia, Vancouver, BC V6T 1Z4, Canada ; Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada ; Department of Biochemistry & Molecular Biology, University of British Colombia, Vancouver, BC V6T 2A1, Canada.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Lamour
authors.affiliation: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK.
authors.forename: Julius B
authors.initials: JB
authors.lastname: Kirkegaard
authors.affiliation: Department of Biochemistry & Molecular Biology, University of British Colombia, Vancouver, BC V6T 2A1, Canada.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK.
authors.forename: Tuomas Pj
authors.initials: TP
authors.lastname: Knowles
authors.affiliation: Centre for High-Throughput Biology, University of British Colombia, Vancouver, BC V6T 1Z4, Canada ; Department of Biochemistry & Molecular Biology, University of British Colombia, Vancouver, BC V6T 2A1, Canada.
authors.forename: Jörg
authors.initials: J
authors.lastname: Gsponer
keywords: AFM; GUI; Matlab; Persistence length; Polymer; Worm-like chain model; Young’s modulus
references.citation: Phys Rev Lett. 2005 Oct 7;95(15):158105
references.pmid: 16241768
references.citation: ACS Nano. 2014 Apr 22;8(4):3851-61
references.pmid: 24588725
references.citation: Nat Nanotechnol. 2011 Jul 31;6(8):469-79
references.pmid: 21804553
references.citation: J Cell Biol. 1993 Feb;120(4):923-34
references.pmid: 8432732
references.citation: J Mol Biol. 1996 Dec 20;264(5):919-32
references.pmid: 9000621