title: Lr70, a new gene for leaf rust resistance mapped in common wheat accession KU3198.
abstract: KU3198 is a common wheat accession that carries one novel leaf rust resistance (Lr) gene, Lr70 , and another Lr gene which is either novel, Lr52 or an allele of Lr52. Leaf rust, caused by Puccinia triticina Eriks. (Pt), is a broadly distributed and economically important disease of wheat. Deploying cultivars carrying effective leaf rust resistance (Lr) genes is a desirable method of disease control. KU3198 is a common wheat (Triticum aestivum L.) accession from the Kyoto collection that was highly resistant to Pt in Canada. An F2 population from the cross HY644/KU3198 showed segregation for two dominant Lr genes when tested with Pt race MBDS which was virulent on HY644. Multiple bulk segregant analysis (MBSA) was employed to find putative chromosome locations of these Lr genes using SSR markers that provided coverage of the genome. MBSA predicted that the Lr genes were located on chromosomes 5B and 5D. A doubled haploid population was generated from the cross of JBT05-714 (HY644*3/KU3198), a line carrying one of the Lr genes from KU3198, to Thatcher. This population segregated for a single Lr gene conferring resistance to Pt race MBDS, which was mapped to the terminal region of the short arm of chromosome 5B with SSR markers and given the temporary designation LrK1. One F3 family derived from the HY644/KU3198 F2 population that segregated only for the second Lr gene from KU3198 was identified. This family was treated as an F2-equivalent population and used for mapping the Lr gene, which was located to the terminal region of chromosome 5DS. As no other Lr gene has been mapped to 5DS, this gene is novel and has been designated as Lr70.
authors.affiliation: Agriculture and Agri-Food Canada, Cereal Research Centre, 101 Route 100 Unit 100, Morden, MB, R6M 1Y5, Canada, colin.hiebert@agr.gc.ca.
authors.forename: Colin W
authors.initials: CW
authors.lastname: Hiebert
authors.forename: Brent D
authors.initials: BD
authors.lastname: McCallum
authors.forename: Julian B
authors.initials: JB
authors.lastname: Thomas
mesh_terms: D001487:Basidiomycota / Q000472:pathogenicity*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
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