title: Generation and characterization of Lhx9-GFPCreER(T2) knock-in mouse line.
abstract: LHX9 is a LIM-homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an Lhx9-GFPCreER(T) (2) (GCE) knock-in mouse line by knocking-in a GCE cassette at the Lhx9 locus, thus inactivating endogenous Lhx9. Lhx9(GCE) (/+) mice were viable, fertile, and displayed no overt phenotypical characteristics. Lhx9(GCE) (/) (GCE) mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the Lhx9-GCE mouse line was verified by crossing it to a Rosa26-tdTomato reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the Lhx9-GCE mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments. genesis
authors.affiliation: Department of Ophthalmology and Flaum Eye Institute, University of Rochester, Rochester, NY 14642.|Department of Neurobiology and Anatomy, University of Rochester, Rochester, NY 14642.
authors.forename: Revathi
authors.initials: R
authors.lastname: Balasubramanian
authors.affiliation: Department of Biology, University of Rochester Medical Center, Rochester, NY 14642, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Bui
authors.affiliation: Department of Ophthalmology and Flaum Eye Institute, University of Rochester, Rochester, NY 14642.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Ophthalmology and Flaum Eye Institute, University of Rochester, Rochester, NY 14642.
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.affiliation: Department of Ophthalmology and Flaum Eye Institute, University of Rochester, Rochester, NY 14642.|Department of Neurobiology and Anatomy, University of Rochester, Rochester, NY 14642.
authors.forename: Lin
authors.initials: L
authors.lastname: Gan
mesh_terms: D025042:Amacrine Cells / Q000166:cytology / Q000378:metabolism; D000818:Animals; D005121:Extremities / Q000196:embryology / Q000254:growth & development; D005260:Female; D055879:Gene Knock-In Techniques; D056426:Genetic Loci; D006624:Hippocampus / Q000196:embryology / Q000254:growth & development; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D060850:LIM-Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D009474:Neurons / Q000378:metabolism / Q000382:microbiology; D012160:Retina / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D013737:Testis / Q000196:embryology / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D060850:LIM-Homeodomain Proteins; C117058:Lhx9 protein, mouse; D014157:Transcription Factors; C045073:Cre recombinase; D019426:Integrases
keywords: Cre recombinase; LIM-homeodomain; Lhx9; amacrine cells; retina; transcription factor
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