title: Fabrication of optical multilayer for two-color phase plate in super-resolution microscope.
abstract: In super-resolution microscopy based on fluorescence depletion, the two-color phase plate (TPP) is an indispensable optical element, which can independently control the phase shifts for two beams of different color, i.e., the pump and erase beams. By controlling a phase shift of the erase beam through the TPP, the erase beam can be modulated into a doughnut shape, while the pump beam maintains the initial Gaussian shape. To obtain a reliable optical multiplayer (ML) for the TPP, we designed a ML with only two optical layers by performing numerical optimization. The measured phase shifts generated by the fabricated ML using interferometry correspond to the design values. The beam profiles in the focal plane are also consistent with theoretical results. Although the fabricated ML consists of only two optical layers, the ML can provide a suitable phase modulation function for the TPP in a practical super-resolution microscope.
authors.affiliation: Olympus Corporation, 2-3 Kuboyama-cho, Hachioji 192-8512, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Iketaki
authors.affiliation: Toray Engineering Co. Ltd., 1-1-45 Oe, Otsu 520-2141, Japan.
authors.forename: Katsuichi
authors.initials: K
authors.lastname: Kitagawa
authors.affiliation: Ceratech Japan Co., Ltd., 500 Okada, Shinonoi, Nagano 381-2295, Japan.
authors.forename: Kohjiro
authors.initials: K
authors.lastname: Hidaka
authors.affiliation: Ceratech Japan Co., Ltd., 500 Okada, Shinonoi, Nagano 381-2295, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Kato
authors.affiliation: Ceratech Japan Co., Ltd., 500 Okada, Shinonoi, Nagano 381-2295, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hirabayashi
authors.affiliation: Department of Physics, Budapest University of Technology and Economics, Budapest 1111, Hungary.
authors.forename: Nandor
authors.initials: N
authors.lastname: Bokor