title: Effects of Nano-MnO2 on dopaminergic neurons and the spatial learning capability of rats.
abstract: This study aimed to observe the effect of intracerebrally injected nano-MnO2 on neurobehavior and the functions of dopaminergic neurons and astrocytes. Nano-MnO2, 6-OHDA, and saline (control) were injected in the substantia nigra and the ventral tegmental area of Sprague-Dawley rat brains. The neurobehavior of rats was evaluated by Morris water maze test. Tyrosine hydroxylase (TH), inducible nitric oxide synthase (iNOS) and glial fibrillary acidic protein (GFAP) expressions in rat brain were detected by immunohistochemistry. Results showed that the escape latencies of nano-MnO2 treated rat increased significantly compared with control. The number of TH-positive cells decreased, GFAP- and iNOS-positive cells increased significantly in the lesion side of the rat brains compared with the contralateral area in nano-MnO2 group. The same tendencies were observed in nano-MnO2-injected rat brains compared with control. However, in the the positive control, 6-OHDA group, escape latencies increased, TH-positive cell number decreased significantly compared with nano-MnO2 group. The alteration of spatial learning abilities of rats induced by nano-MnO2 may be associated with dopaminergic neuronal dysfunction and astrocyte activation.
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. llg501@aliyun.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Institute of Neurobiology, Southeast University, Nanjing 210009, China. shitingting.student@sina.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Shi
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. lixiaobo1007@hotmail.com.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Neurobiology, Southeast University, Nanjing 210009, China. zsl@seu.edu.cn.
authors.forename: Shuilin
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. lhyin@seu.edu.cn.
authors.forename: Lihong
authors.initials: L
authors.lastname: Yin
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. yppu@seu.edu.cn.
authors.forename: Yuepu
authors.initials: Y
authors.lastname: Pu
mesh_terms: D000818:Animals; D002452:Cell Count; D059290:Dopaminergic Neurons / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D017895:Manganese Compounds; D018782:Maze Learning / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000633:toxicity*; D010087:Oxides / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000187:drug effects*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D014446:Tyrosine 3-Monooxygenase
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