title: In vitro and in vivo effects of sublethal cadmium on the expression of MT2 and ABCC2 genes in grass carp (Ctenopharyngodon idellus).
abstract: To gain more knowledge about the physiological regulation of metal pollutant detoxification in grass carp, we examined Cd concentration and its the potential influence on the expression of metallothionein 2 (MT2) and multidrug resistance protein 2 (ABCC2) mRNA in the liver and kidney, using in vitro and in vivo experiments. First, the full-length of MT2 cDNA and partial ABCC2 cDNA was obtained, consisting 183bp and 366bp respectively. In vivo approach, grass carp received 96h exposure of Cd (1/10 LD50), and MT2 and ABCC2 mRNA expression were determined by qRT-PCR. The Cd treatment resulted in an increase of MT2 mRNA level in the liver with Cd accumulation. Nonetheless, the elevation ABCC2 mRNA in the liver was appeared at 48h after Cd exposure, as well as the expression of MT2 and ABCC2 mRNA in the kidney. The in vitro experiment was carried out using the hepatocyte (L86) and nephroblasts (CIK). The qRT-RCR results showed that MT2 and ABCC2 mRNA dramatically increased following Cd exposure (1/10 LD50); however, ABCC2 mRNA expression was suppressed in the L86 cell line at first (6h). In conclusion, this result suggested that both MT2 and ABCC2 mRNA may play important roles in the detoxification of toxic metals, and MT2 gene was more sensitive to Cd induction.
authors.affiliation: College of Life Science, Foshan University, No. 1 Xianhu University Road, Nanhai, Foshan, Guangdong 528231, China; Holdone Aquaculture Breeding Limited Company, Foshan, Guangdong 528231, China.
authors.forename: Shuwen
authors.initials: S
authors.lastname: Tan
authors.affiliation: College of Life Science, Foshan University, No. 1 Xianhu University Road, Nanhai, Foshan, Guangdong 528231, China; Holdone Aquaculture Breeding Limited Company, Foshan, Guangdong 528231, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Biophotonics, South China Normal University, Guangzhou, Guangdong 510631, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Life Science, Foshan University, No. 1 Xianhu University Road, Nanhai, Foshan, Guangdong 528231, China; Holdone Aquaculture Breeding Limited Company, Foshan, Guangdong 528231, China. Electronic address: yu71hui@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D002347:Carps / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D008658:Inactivation, Metabolic; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation
chemical_list: D002104:Cadmium; D018076:DNA, Complementary; D008668:Metallothionein; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger
keywords: ABCC2 gene; Cadmium; Detoxification mechanism; Grass carp; MT2 gene