title: The effects of human keratinocyte coculture on human adipose-derived stem cells.
abstract: The potential for adipose-derived stem cells to differentiate into keratinocyte-like cells has recently been receiving attention, stemming from the hypothesis that a bioengineered skin may be manufactured from these readily available mesenchymal stem cells. This study was conducted to evaluate the influence of human keratinocyte non-contact coculture on hADSCs. Human epidermal keratinocytes and hADSCs obtained by lipoaspiration were cultured in keratinogenic growth media, which were divided into the following groups: human adipose-derived stem cell (hADSC) monoculture, non-contact coculture of hADSCs and human keratinocytes and keratinocyte monoculture. Cell proliferation was assessed, and keratogenicity was analysed through immunocytochemistry and polymerase chain reaction of early, intermediate and late keratogenic markers. hADSCs cocultured with keratinocytes displayed enhanced proliferation compared with the monoculture group. After a 7-day coculture period, immunohistochemistry and polymerase chain reaction findings revealed the presence of specific keratinocyte markers in the coculture group. This study demonstrates that hADSCs cocultured with keratinocytes have the capacity to transdifferentiate into keratinocyte lineage cells, and suggests that adipose tissue may be a source of keratinocytes that may further be used in structuring the bioengineered skin.
authors.affiliation: Department of Plastic and Reconstructive Surgery, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Bommie F
authors.initials: BF
authors.lastname: Seo
authors.affiliation: Department of Plastic and Reconstructive Surgery, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Ki J
authors.initials: KJ
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Min K
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, College of Medicine, The Catholic University of Korea, Seoul, South Korea. rhie@catholic.ac.kr.
authors.forename: Jong W
authors.initials: JW
authors.lastname: Rhie
mesh_terms: D000273:Adipose Tissue; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D006801:Humans; D015603:Keratinocytes; D059630:Mesenchymal Stem Cells
keywords: Adipose-derived stem cells; Adult stem cells; Coculture; Differentiation; Keratinocytes
references.citation: Burns. 2014 Feb;40(1):82-8
references.pmid: 23764150
references.citation: Cytotherapy. 2013 Apr;15(4):434-48
references.pmid: 23376106
references.citation: FASEB J. 2011 Jan;25(1):122-31
references.pmid: 20847231
references.citation: Transl Res. 2014 Apr;163(4):399-408
references.pmid: 24361334
references.citation: Differentiation. 2007 Apr;75(4):292-8
references.pmid: 17286607
references.citation: J Biomech Eng. 2000 Dec;122(6):570-5
references.pmid: 11192376
references.citation: J Clin Invest. 2004 Jun;113(12):1701-10
references.pmid: 15199405
references.citation: Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2397-402
references.pmid: 12606728
references.citation: Br J Dermatol. 1993 Aug;129(2):110-9
references.pmid: 7544603
references.citation: Sci STKE. 2005 Jul 26;2005(294):pe37
references.pmid: 16046665
references.citation: PLoS One. 2013 Dec 02;8(12):e80587
references.pmid: 24312483
references.citation: J Invest Dermatol. 1983 Jul;81(1 Suppl):100s-3s
references.pmid: 6345687
references.citation: J Immunol. 2008 Feb 15;180(4):2581-7
references.pmid: 18250469
references.citation: Toxicol In Vitro. 2011 Sep;25(6):1224-30
references.pmid: 21645610
references.citation: Surg Today. 2011 Jan;41(1):18-23
references.pmid: 21191687
references.citation: Burns. 2007 Jun;33(4):418-28
references.pmid: 17467179
references.citation: Nat Cell Biol. 2004 Jun;6(6):532-9
references.pmid: 15133469
references.citation: Cell Tissue Res. 2012 Jan 31;347(3):737-746
references.pmid: 22290635