title: Identification of a novel 6'-N-aminoglycoside acetyltransferase, AAC(6')-Iak, from a multidrug-resistant clinical isolate of Stenotrophomonas maltophilia.
abstract: Stenotrophomonas maltophilia IOMTU250 has a novel 6'-N-aminoglycoside acetyltransferase-encoding gene, aac(6')-Iak. The encoded protein, AAC(6')-Iak, consists of 153 amino acids and has 86.3% identity to AAC(6')-Iz. Escherichia coli transformed with a plasmid containing aac(6')-Iak exhibited decreased susceptibility to arbekacin, dibekacin, neomycin, netilmicin, sisomicin, and tobramycin. Thin-layer chromatography showed that AAC(6')-Iak acetylated amikacin, arbekacin, dibekacin, isepamicin, kanamycin, neomycin, netilmicin, sisomicin, and tobramycin but not apramycin, gentamicin, or lividomycin.
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tada
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University, Maharajgunj, Kathmandu, Nepal.
authors.forename: Rajan K
authors.initials: RK
authors.lastname: Dahal
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University, Maharajgunj, Kathmandu, Nepal.
authors.forename: Shyam K
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Shimada
authors.affiliation: Department of International Medical Cooperation, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohara
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan tkirikae@ri.imcj.go.jp.
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University, Maharajgunj, Kathmandu, Nepal.
authors.forename: Bharat M
authors.initials: BM
authors.lastname: Pokhrel
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003982:Dibekacin / Q000031:analogs & derivatives / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D008826:Microbial Sensitivity Tests; D009355:Neomycin / Q000494:pharmacology; D009428:Netilmicin / Q000494:pharmacology; D012853:Sisomicin / Q000494:pharmacology; D020615:Stenotrophomonas maltophilia / Q000187:drug effects* / Q000201:enzymology*; D014031:Tobramycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003982:Dibekacin; D009428:Netilmicin; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; C031986:arbekacin; D009355:Neomycin; D014031:Tobramycin; D012853:Sisomicin
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