title: Pre-stimulation of the kallikrein system in cisplatin-induced acute renal injury: an approach to renoprotection.
abstract: Antineoplastic treatment with cisplatin is frequently complicated by nephrotoxicity. Although oxidative stress may be involved, the pathogenic mechanisms responsible for renal damage have not been completely clarified. In order to investigate the role of the renal kinin system in this condition, a group of rats was submitted to high potassium diet to stimulate the synthesis and excretion of tissue kallikrein 1 (rKLK1) previous to an intraperitoneal injection of 7 mg/kg cisplatin. A significant reduction in lipoperoxidation, evidenced by urinary excretion of malondialdehyde and renal immunostaining of hidroxy-nonenal, was accompanied by a decline in apoptosis. Coincident with these findings we observed a reduction in the expression of renal KIM-1 suggesting that renoprotection may be occurring. Stimulation or indemnity of the renal kinin system deserves to be evaluated as a complementary pharmacological measure to diminish cisplatin nephrotoxicity.
authors.affiliation: Program of M.Sc., Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Andrés
authors.initials: A
authors.lastname: Aburto
authors.affiliation: School of Biochemistry, Faculty of Sciences, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Agustín
authors.initials: A
authors.lastname: Barría
authors.affiliation: Ph.D. Program, Faculty of Sciences, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Areli
authors.initials: A
authors.lastname: Cárdenas
authors.affiliation: Department of Anatomy, Histology and Pathology, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Daniel
authors.initials: D
authors.lastname: Carpio
authors.affiliation: Department of Anatomy, Histology and Pathology, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Carlos D
authors.initials: CD
authors.lastname: Figueroa
authors.affiliation: Department of Nephrology, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Burgos
authors.affiliation: Department of Nephrology, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile. Electronic address: leopoldoardiles@gmail.com.
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Ardiles
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced*; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D015815:Cell Adhesion Molecules / Q000032:analysis; D002945:Cisplatin / Q000633:toxicity*; D007610:Kallikreins / Q000502:physiology*; D007668:Kidney / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D000072105:Superoxide Dismutase-1
chemical_list: D000970:Antineoplastic Agents; D015815:Cell Adhesion Molecules; D002945:Cisplatin; D007610:Kallikreins; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; C110936:Havcr1 protein, rat; C000606292:Sod1 protein, rat
keywords: Apoptosis; Cisplatin; Kallikrein; Nephrotoxicity; Oxidative stress