title: Lawsonia inermis-mediated synthesis of silver nanoparticles: activity against human pathogenic fungi and bacteria with special reference to formulation of an antimicrobial nanogel.
abstract: Lawsonia inermis mediated synthesis of silver nanoparticles (Ag-NPs) and its efficacy against Candida albicans, Microsporum canis, Propioniabacterium acne and Trichophyton mentagrophytes is reported. A two-step mechanism has been proposed for bioreduction and formation of an intermediate complex leading to the synthesis of capped nanoparticles was developed. In addition, antimicrobial gel for M. canis and T. mentagrophytes was also formulated. Ag-NPs were synthesized by challenging the leaft extract of L. inermis with 1 mM AgNO₃. The Ag-NPs were characterized by Ultraviolet-Visible (UV-Vis) spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Transmission electron microscopy (TEM), nanoparticle tracking and analysis sytem (NTA) and zeta potential was measured to detect the size of Ag-NPs. The antimicrobial activity of Ag-NPs was evaluated by disc diffusion method against the test organisms. Thus these Ag-NPs may prove as a better candidate drug due to their biogenic nature. Moreover, Ag-NPs may be an answer to the drug-resistant microorganisms.
authors.affiliation: Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602, Maharashtra, India.
authors.forename: Arpita
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602, Maharashtra, India.
authors.forename: Shital R
authors.initials: SR
authors.lastname: Bonde
authors.affiliation: Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602, Maharashtra, India.
authors.forename: Swapnil
authors.initials: S
authors.lastname: Gaikwad
authors.affiliation: Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602, Maharashtra, India.
authors.forename: Avinash
authors.initials: A
authors.lastname: Ingle
authors.affiliation: Department of Biology, Utah State University, Logan, UT 84322-5305, USA.
authors.forename: Aniket K
authors.initials: AK
authors.lastname: Gade
authors.affiliation: Laboratório de Química Biológica, Instituto de Química - Unicamp Cidade Universitária 'Zefferino Vaz', Barão Geraldo-CEP 13083-970, Caixa Postal 6150 Campinas, SP, Brazil. mkrai123@rediffmail.com.
authors.forename: Mahendra
authors.initials: M
authors.lastname: Rai
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000494:pharmacology; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004339:Drug Compounding / Q000379:methods; D005658:Fungi / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts; D012834:Silver