title: Diversity analysis in Indian genotypes of linseed (Linum usitatissimum L.) using AFLP markers.
abstract: AFLP fingerprinting of 45 Indian genotypes of linseed was carried out to determine the genetic relationship among them. Sixteen primer combinations produced 1142 fragments with 1129 as polymorphic and 13 as monomorphic fragments. Polymorphic fragments varied from 44 (E-ACA/M-CTA) to 94 (E-AGC/M-CAC) with an average of 70.6 fragments per primer combination. The frequency of polymorphism varied from 93.7% to 100% with an average of 98.8% across all the genotypes. The PIC value ranged from 0.19 to 0.31 with an average of 0.23 per primer combination. The primer pair E-AGC/M-CAC showed the maximum PIC value (0.31) followed by E-AGC/M-CAG (0.29), E-AAC/M-CAG (0.26) and E-AGC/M-CTA (0.25). Resolving power (RP) and marker index (MI) varied from 13.73 to 43.50 and 8.81 to 28.91 respectively. The Jaccard's similarity coefficient varied from 0.16 to 0.57 with an average of 0.26 ± 0.05. The maximum genetic similarities (57%) were detected between genotypes Him Alsi-1 and Him Alsi-2, followed by Him Alsi-1 and GS41 and GS41 and LC-54. The genotypes R-552, Himani, RKY-14, Meera, Indira Alsi-32 and Suyog were found to be more divergent genotypes. The NJ clustering grouped all the 45 genotypes into three major clusters. In general the genotypes of cluster III had high oil content and those of cluster I had low oil content. At the population level, within population variance was much higher than between populations variance.
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
authors.lastname: Chandrawati
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, India.
authors.forename: Ramanuj
authors.initials: R
authors.lastname: Maurya
authors.affiliation: AICRP on Linseed, CSAUA&T, Kanpur, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Singh
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
authors.forename: S A
authors.initials: SA
authors.lastname: Ranade
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India. Electronic address: h.yadav@nbri.res.in.
authors.forename: Hemant Kumar
authors.initials: HK
authors.lastname: Yadav
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000704:Analysis of Variance; D016000:Cluster Analysis; D017931:DNA Primers; D019597:Flax / Q000145:classification / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D014644:Genetic Variation; D018745:Genome, Plant; D005838:Genotype; D018515:Plant Leaves / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D017931:DNA Primers; D005819:Genetic Markers
keywords: AFLP; Flax; Genetic diversity; Jaccard's coefficient; Polymorphism information content