title: In vitro PAMAM, phosphorus and viologen-phosphorus dendrimers prevent rotenone-induced cell damage.
abstract: We have investigated whether polyamidoamine (PAMAM), phosphorus (pd) and viologen-phosphorus (vpd) dendrimers can prevent damage to embryonic mouse hippocampal cells (mHippoE-18) caused by rotenone, which is used as a pesticide, insecticide, and as a nonselective piscicide, that works by interfering with the electron transport chain in mitochondria. Several basic aspects, such as cell viability, production of reactive oxygen species and changes in mitochondrial transmembrane potential, were analyzed. mHippoE-18 cells were treated with these structurally different dendrimers at 0.1μM. A 1h incubation with dendrimers was followed by the addition of rotenone at 1μM, and a further 24h incubation. PAMAM, phosphorus and viologen-phosphorus dendrimers all increased cell viability (reduced cell death-data need to be compared with untreated controls). A lower level of reactive oxygen species and a favorable effect on mitochondrial system were found with PAMAM and viologen-phosphorus dendrimers. These results indicate reduced toxicity in the presence of dendrimers.
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland. Electronic address: milowska@biol.uni.lodz.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Milowska
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Szwed
authors.affiliation: Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Zablocka
authors.affiliation: Laboratoire de Chimie de Coordination CNRS, 205 route de Narbonne, 31077 Toulouse, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Caminade
authors.affiliation: Laboratoire de Chimie de Coordination CNRS, 205 route de Narbonne, 31077 Toulouse, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Majoral
authors.affiliation: Université Paris Descartes, PRES Sorbonne Paris Cité, CNRS UMR 860, Laboratoire de Chimie et de Biochimie pharmacologiques et toxicologique, 45 rue des Saints Pères, 75006 Paris, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Mignani
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Gabryelak
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D050091:Dendrimers / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D010758:Phosphorus / Q000737:chemistry / Q000494:pharmacology*; D011073:Polyamines / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012402:Rotenone / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014755:Viologens / Q000494:pharmacology*
chemical_list: D050091:Dendrimers; C531249:Poly(amidoamine); D011073:Polyamines; D017382:Reactive Oxygen Species; D014755:Viologens; D012402:Rotenone; D010758:Phosphorus
keywords: Cytotoxicity; Dendrimers; Parkinson’s disease; Rotenone