title: Communities of sediment ammonia-oxidizing bacteria along a coastal pollution gradient in the East China Sea.
abstract: Anthropogenic nitrogen (N) discharges has caused eutrophication in coastal zones. Ammonia-oxidizing bacteria (AOB) convert ammonia to nitrite and play important roles in N transformation. Here, we used pyrosequencing based on the amoA gene to investigate the response of the sediment AOB community to an N pollution gradient in the East China Sea. The results showed that AOB assemblages were primarily affiliated with Nitrosospira-like lineages, and only 0.4% of those belonged to Nitrosomonas-like lineage. The Nitrosospira-like lineage was separated into four clusters that were most similar to the sediment AOB communities detected in adjacent marine regions. Additionally, one clade was out grouped from the AOB lineages, which shared the high similarities with pmoA gene. The AOB community structures substantially changed along the pollution gradient, which were primarily shaped by NH4(+)-N, NO3(-)-N, SO4(2)(-)-S, TP and Eh. These results demonstrated that coastal pollution could dramatically influence AOB communities, which, in turn, may change ecosystem function.
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Manhua
authors.initials: M
authors.lastname: Hou
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China; 2011 Center of Modern Marine Aquaculture of East China Sea, Ningbo 315211, China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Xiong
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China; 2011 Center of Modern Marine Aquaculture of East China Sea, Ningbo 315211, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Marine Environmental Monitoring Center of Ningbo, SOA, Ningbo 315012, China.
authors.forename: Xiansen
authors.initials: X
authors.lastname: Ye
authors.affiliation: Marine Environmental Monitoring Center of Ningbo, SOA, Ningbo 315012, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Ye
authors.affiliation: Marine Environmental Monitoring Center of Ningbo, SOA, Ningbo 315012, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Changju
authors.initials: C
authors.lastname: Hu
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China; 2011 Center of Modern Marine Aquaculture of East China Sea, Ningbo 315211, China. Electronic address: zhangdemin@nbu.edu.cn.
authors.forename: Demin
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000641:Ammonia; D001105:Archaea / Q000235:genetics; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics / Q000502:physiology*; D002681:China; D017753:Ecosystem; D005068:Eutrophication; D019015:Geologic Sediments / Q000382:microbiology*; D009584:Nitrogen; D009792:Oceans and Seas; D010802:Phylogeny; D014876:Water Pollution / Q000032:analysis
chemical_list: D000641:Ammonia; D009584:Nitrogen
keywords: Ammonia oxidizing bacteria; Nitrification; Pyrosequencing; amoA