title: Pseudomonas soli sp. nov., a novel producer of xantholysin congeners.
abstract: A chemoorganotrophic Gram-negative bacterium was isolated by means of a diffusion sandwich system from a soil sample from the Sierra Nevada National Park, Spain. Strain F-279,208(T) was oxidase and catalase positive, strictly aerobic, non-spore-forming and motile by single polar flagellum. Phylogenetic analysis of the 16S rRNA, gyrB, rpoB and rpoD genes revealed that strain F-279,208(T) belongs to the Pseudomonas putida group with Pseudomonas mosselii and Pseudomonas entomophila as its closest relatives. DNA-DNA hybridization assays and phenotypic traits confirmed that this strain belongs to a novel species of the genus Pseudomonas, for which the name Pseudomonas soli sp. nov. is proposed. The type strain is F-279,208(T) (=DSM 28043(T)=LMG 27941(T)), and during fermentation it produces xantholysins, a family of lipodepsipeptides. The major compound, xantholysin A, showed an interesting activity in a RCC4 kidney tumor cell line with inactivation of VHL linked with the HIF pathway, without any cytotoxic effects against other human tumor cell lines tested including, liver, pancreas and breast.
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Pascual
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: García-López
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Carmona
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain.
authors.forename: Thiciana da S
authors.initials: Tda S
authors.lastname: Sousa
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: de Pedro
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain.
authors.forename: Bastien
authors.initials: B
authors.lastname: Cautain
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Martín
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Vicente
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Reyes
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain.
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Bills
authors.affiliation: Fundación MEDINA, Avenida del Conocimiento, 3. Parque Tecnológico de Ciencias de la Salud, E-18016 Granada, Spain. Electronic address: olga.genilloud@medinaandalucia.es.
authors.forename: Olga
authors.initials: O
authors.lastname: Genilloud
mesh_terms: D045744:Cell Line, Tumor; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Cytotoxic activity; Diffusion sandwich system; HIF pathway; Lipodepsipeptide; Multilocus sequence analysis; Pseudomonas soli sp. nov.; Sierra Nevada National Park; Xantholysin