title: Metabotropic glutamate receptor 1 recycles to the cell surface in protein phosphatase 2A-dependent manner in non-neuronal and neuronal cell lines.
abstract: Trafficking of G protein-coupled receptors plays a crucial role in controlling the precise signalling of the receptor as well as its proper regulation. Metabotropic glutamate receptor 1 (mGluR1), a G protein-coupled receptor, is a member of the group I mGluR family. mGluR1 plays a critical role in neuronal circuit formation and also in multiple types of synaptic plasticity. This receptor has also been reported to be involved in various neuropsychiatric diseases. Other than the central nervous system, mGluR1 plays crucial roles in various non-neuronal cells like hepatocytes, skin cells, etc. Although it has been reported that mGluR1 gets endocytosed on ligand application, the events after the internalization of the receptor has not been studied. We show here that mGluR1 internalizes on ligand application. Subsequent to endocytosis, majority of the receptors localize at the recycling compartment and no significant presence of the receptor was noticed in the lysosome. Furthermore, mGluR1 returned to the cell membrane subsequent to ligand-mediated internalization. We also show here that the recycling of mGluR1 is dependent on the activity of protein phosphatase 2A. Thus, our data suggest that the ligand-mediated internalized receptors recycle back to the cell surface in protein phosphatase 2A-dependent manner.
authors.affiliation: Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Punjab, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Pandey
authors.forename: Prabhat Kumar
authors.initials: PK
authors.lastname: Mahato
authors.forename: Samarjit
authors.initials: S
authors.lastname: Bhattacharyya
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D065095:Calcineurin Inhibitors / Q000494:pharmacology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D016572:Cyclosporine / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D054648:Protein Phosphatase 2 / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D018094:Receptors, Metabotropic Glutamate / Q000235:genetics / Q000378:metabolism*; D016559:Tacrolimus / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection
chemical_list: D065095:Calcineurin Inhibitors; D034741:RNA, Small Interfering; D018094:Receptors, Metabotropic Glutamate; C104077:metabotropic glutamate receptor type 1; D000643:Ammonium Chloride; D008734:Methoxyhydroxyphenylglycol; D016572:Cyclosporine; D054648:Protein Phosphatase 2; C010117:3,4-dihydroxyphenylglycol; D016559:Tacrolimus
keywords: GPCR; endocytosis; metabotropic glutamate receptors; neurotransmitter receptors; receptor recycling; trafficking