title: Antimycobacterial, antimicrobial, and biocompatibility properties of para-aminosalicylic acid with zinc layered hydroxide and Zn/Al layered double hydroxide nanocomposites.
abstract: The treatment of tuberculosis by chemotherapy is complicated due to multiple drug prescriptions, long treatment duration, and adverse side effects. We report here for the first time an in vitro therapeutic effect of nanocomposites based on para-aminosalicylic acid with zinc layered hydroxide (PAS-ZLH) and zinc-aluminum layered double hydroxides (PAS-Zn/Al LDH), against mycobacteria, Gram-positive bacteria, and Gram-negative bacteria. The nanocomposites demonstrated good antimycobacterial activity and were found to be effective in killing Gram-positive and Gram-negative bacteria. A biocompatibility study revealed good biocompatibility of the PAS-ZLH nanocomposites against normal human MRC-5 lung cells. The para-aminosalicylic acid loading was quantified with high-performance liquid chromatography analysis. In summary, the present preliminary in vitro studies are highly encouraging for further in vivo studies of PAS-ZLH and PAS-Zn/Al LDH nanocomposites to treat tuberculosis.
authors.affiliation: Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Bullo
authors.initials: B
authors.lastname: Saifullah
authors.affiliation: Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia ; Department of Environmental Health, Faculty of Public Health and Tropical Medicine, Jazan University, Jazan, Saudi Arabia.
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: El Zowalaty
authors.affiliation: Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Palanisamy
authors.initials: P
authors.lastname: Arulselvan
authors.affiliation: Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia ; Department of Human Anatomy, Faculty of Medicine and Health Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Sharida
authors.initials: S
authors.lastname: Fakurazi
authors.affiliation: Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA ; Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
authors.affiliation: Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA.
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Geilich
authors.affiliation: Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Mohd Zobir
authors.initials: MZ
authors.lastname: Hussein
mesh_terms: D000535:Aluminum / Q000737:chemistry; D010131:Aminosalicylic Acid / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D006878:Hydroxides / Q000737:chemistry; D008168:Lung / Q000166:cytology; D009161:Mycobacterium / Q000187:drug effects; D053761:Nanocomposites; D015032:Zinc / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000995:Antitubercular Agents; D006878:Hydroxides; D010131:Aminosalicylic Acid; D000535:Aluminum; D015032:Zinc
keywords: Zn/Al-layered double hydroxides; antimicrobial agents; para-aminosalicylic acid (PAS); tuberculosis; zinc layered hydroxides
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