title: Differential expression of immunogenic proteins on virulent Mycobacterium tuberculosis clinical isolates.
abstract: Molecular epidemiology has revealed that Mycobacterium tuberculosis (Mtb), formerly regarded as highly conserved species, displays a considerable degree of genetic variability that can influence the outcome of the disease as well as the innate and adaptive immune response. Recent studies have demonstrated that Mtb families found worldwide today differ in pathology, transmissibility, virulence, and development of immune response. By proteomic approaches seven proteins that were differentially expressed between a local clinical isolate from Latin-American-Mediterranean (LAM) and from Haarlem (H) lineages were identified. In order to analyze the immunogenic ability, recombinant Rv2241, Rv0009, Rv0407, and Rv2624c proteins were produced for testing specific antibody responses. We found that these proteins induced humoral immune responses in patients with drug-sensitive and drug-resistant tuberculosis with substantial cross-reactivity among the four proteins. Moreover, such reactivity was also correlated with anti-Mtb-cell surface IgM, but not with anti-ManLAM, anti-PPD, or anti-Mtb-surface IgG antibodies. Therefore, the present results describe new Mtb antigens with potential application as biomarkers of TB.
authors.affiliation: IMEX-CONICET, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425 CABA, Argentina.
authors.forename: Pablo
authors.identifier: 0000-0002-8725-6281
authors.initials: P
authors.lastname: Schierloh
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and Los Reseros s/n, 1686 Hurlingham, Argentina.
authors.forename: Laura
authors.initials: L
authors.lastname: Klepp
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and Los Reseros s/n, 1686 Hurlingham, Argentina.
authors.forename: Camila
authors.initials: C
authors.lastname: Vazquez
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and Los Reseros s/n, 1686 Hurlingham, Argentina.
authors.forename: Roxana Valeria
authors.initials: RV
authors.lastname: Rocha
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and Los Reseros s/n, 1686 Hurlingham, Argentina.
authors.forename: Federico Carlos
authors.initials: FC
authors.lastname: Blanco
authors.affiliation: IMEX-CONICET, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425 CABA, Argentina.
authors.forename: Luciana
authors.identifier: 0000-0001-7045-3572
authors.initials: L
authors.lastname: Balboa
authors.affiliation: Servicio de Micobacterias, Instituto Nacional de Enfermedades Infecciosas ANLIS "Carlos G. Malbran", Avenida Vélez Sarsfield 563, 1281 CABA, Argentina.
authors.forename: Beatriz
authors.initials: B
authors.lastname: López
authors.affiliation: Servicio de Micobacterias, Instituto Nacional de Enfermedades Infecciosas ANLIS "Carlos G. Malbran", Avenida Vélez Sarsfield 563, 1281 CABA, Argentina.
authors.forename: Viviana
authors.identifier: 0000-0003-1217-0229
authors.initials: V
authors.lastname: Ritacco
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and Los Reseros s/n, 1686 Hurlingham, Argentina.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Bigi
authors.affiliation: IMEX-CONICET, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425 CABA, Argentina.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Sasiain
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D003429:Cross Reactions / Q000235:genetics / Q000276:immunology; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006801:Humans; D055261:Immunogenetic Phenomena; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D040901:Proteomics; D018088:Tuberculosis, Multidrug-Resistant / Q000097:blood / Q000235:genetics / Q000276:immunology; D037521:Virulence Factors / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D007075:Immunoglobulin M; D037521:Virulence Factors
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