title: Expression analysis of miRNA and target mRNAs in esophageal cancer.
abstract: We aimed to investigate miRNAs and related mRNAs through a network-based approach in order to learn the crucial role that they play in the biological processes of esophageal cancer. Esophageal squamous-cell carcinoma (ESCC) and adenocarcinoma (EAC)-related miRNA and gene expression data were downloaded from the Gene Expression Omnibus database, and differentially expressed miRNAs and genes were selected. Target genes of differentially expressed miRNAs were predicted and their regulatory networks were constructed. Differentially expressed miRNA analysis selected four miRNAs associated with EAC and ESCC, among which hsa-miR-21 and hsa-miR-202 were shared by both diseases. hsa-miR-202 was reported for the first time to be associated with esophageal cancer in the present study. Differentially expressed miRNA target genes were mainly involved in cancer-related and signal-transduction pathways. Functional categories of these target genes were related to transcriptional regulation. The results may indicate potential target miRNAs and genes for future investigations of esophageal cancer.
authors.affiliation: Oncology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: X R
authors.initials: XR
authors.lastname: Meng
authors.affiliation: Gastrointestinal Surgery Department, People's Hospital of Zhengzhou, Zhengzhou, China.
authors.forename: P
authors.initials: P
authors.lastname: Lu
authors.affiliation: Medical Oncology Department, People's Hospital of Zhengzhou, Zhengzhou, China.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Mei
authors.affiliation: Medical Oncology Department, People's Hospital of Zhengzhou, Zhengzhou, China.
authors.forename: G J
authors.initials: GJ
authors.lastname: Liu
authors.affiliation: Oncology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Q X
authors.initials: QX
authors.lastname: Fan
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D000077277:Esophageal Squamous Cell Carcinoma; D020869:Gene Expression Profiling; D063990:Gene Ontology; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D046228:Microarray Analysis; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D016019:Survival Analysis
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
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