title: Sororin pre-mRNA splicing is required for proper sister chromatid cohesion in human cells.
abstract: Sister chromatid cohesion, which depends on cohesin, is essential for the faithful segregation of replicated chromosomes. Here, we report that splicing complex Prp19 is essential for cohesion in both G2 and mitosis, and consequently for the proper progression of the cell through mitosis. Inactivation of splicing factors SF3a120 and U2AF65 induces similar cohesion defects to Prp19 complex inactivation. Our data indicate that these splicing factors are all required for the accumulation of cohesion factor Sororin, by facilitating the proper splicing of its pre-mRNA. Finally, we show that ectopic expression of Sororin corrects defective cohesion caused by Prp19 complex inactivation. We propose that the Prp19 complex and the splicing machinery contribute to the establishment of cohesion by promoting Sororin accumulation during S phase, and are, therefore, essential to the maintenance of genome stability.
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290, Rennes, France Institut de Génétique et Développement de Rennes Université de Rennes 1, Rennes, France erwan.watrin@univ-rennes1.fr.
authors.forename: Erwan
authors.initials: E
authors.lastname: Watrin
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290, Rennes, France Institut de Génétique et Développement de Rennes Université de Rennes 1, Rennes, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Demidova
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290, Rennes, France Institut de Génétique et Développement de Rennes Université de Rennes 1, Rennes, France.
authors.forename: Tanguy
authors.initials: T
authors.lastname: Watrin
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290, Rennes, France Institut de Génétique et Développement de Rennes Université de Rennes 1, Rennes, France.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Centre National de la Recherche Scientifique, UMR 6290, Rennes, France Institut de Génétique et Développement de Rennes Université de Rennes 1, Rennes, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Prigent
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002842:Chromatids / Q000235:genetics; D020090:Chromosome Segregation / Q000235:genetics; D045643:DNA Repair Enzymes / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D042822:Genomic Instability; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000235:genetics; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing / Q000235:genetics*; D000072260:RNA Splicing Factors; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000037:antagonists & inhibitors; D012261:Ribonucleoproteins / Q000037:antagonists & inhibitors; D000072668:Splicing Factor U2AF
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C500695:CDCA5 protein, human; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D012322:RNA Precursors; D000072260:RNA Splicing Factors; D017413:Ribonucleoprotein, U2 Small Nuclear; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C000607494:U2AF2 protein, human; C088049:splicing factor 3a; D045643:DNA Repair Enzymes; C417041:PRPF19 protein, human
keywords: Prp19 complex; Sororin; genome stability; pre‐mRNA maturation; sister chromatid cohesion
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