title: The winch model can explain both coordinated and uncoordinated stepping of cytoplasmic dynein.
abstract: Cytoplasmic dynein moves processively along microtubules, but the mechanism of how its heads use the energy from ATP hydrolysis, coupled to a linker swing, to achieve directed motion, is still unclear. In this article, we present a theoretical model based on the winch mechanism in which the principal direction of the linker stroke is toward the microtubule-binding domain. When mechanically coupling two identical heads (each with postulated elastic properties and a minimal ATPase cycle), the model reproduces stepping with 8-nm steps (even though the motor itself is much larger), interhead coordination, and processivity, as reported for mammalian dyneins. Furthermore, when we loosen the elastic connection between the heads, the model still shows processive directional stepping, but it becomes uncoordinated and the stepping pattern shows a greater variability, which reproduces the properties of yeast dyneins. Their slower chemical kinetics allows processive motility and a high stall force without the need for coordination.
authors.affiliation: Faculty of Mathematics and Physics, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Andreja
authors.initials: A
authors.lastname: Šarlah
authors.affiliation: Faculty of Mathematics and Physics, University of Ljubljana, Ljubljana, Slovenia; J. Stefan Institute, Ljubljana, Slovenia. Electronic address: andrej.vilfan@ijs.si.
authors.forename: Andrej
authors.initials: A
authors.lastname: Vilfan
mesh_terms: D000595:Amino Acid Sequence; D056445:Cytoplasmic Dyneins / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary
chemical_list: D056445:Cytoplasmic Dyneins
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