title: Structural and thermodynamic properties of water-membrane interphases: significance for peptide/membrane interactions.
abstract: Water appears as a common intermediary in the mechanisms of interaction of proteins and polypeptides with membranes of different lipid composition. In this review, how water modulates the interaction of peptides and proteins with lipid membranes is discussed by correlating the thermodynamic response and the structural changes of water at the membrane interphases. The thermodynamic properties of the lipid-protein interaction are governed by changes in the water activity of monolayers of different lipid composition according to the lateral surface pressure. In this context, different water populations can be characterized below and above the phase transition temperature in relation to the CH₂ conformers' states in the acyl chains. According to water species present at the interphase, lipid membrane acts as a water state regulator, which determines the interfacial water domains in the surface. It is proposed that those domains are formed by the contact between lipids themselves and between lipids and the water phase, which are needed to trigger adsorption-insertion processes. The water domains are essential to maintain functional dynamical properties and are formed by water beyond the hydration shell of the lipid head groups. These confined water domains probably carries information in local units in relation to the lipid composition thus accounting for the link between lipidomics and aquaomics. The analysis of these results contributes to a new insight of the lipid bilayer as a non-autonomous, responsive (reactive) structure that correlates with the dynamical properties of a living system.
authors.affiliation: Centro de Investigaciones y Transferencia Santiago del Estero (CITSE), (CONICET-UNSE), Laboratorio de Biointerfases y Sistemas Biomiméticos, Laboratorios Centrales - Ala Norte, Ruta Nacional 9, Km 1125 - Villa El Zanjón, CP 4206 Santiago del Estero, Argentina. Electronic address: disalvoanibal@yahoo.com.ar.
authors.forename: E A
authors.initials: EA
authors.lastname: Disalvo
authors.affiliation: Department of Pharmaceutical Technology, Universidad de Buenos Aires, Buenos Aires, Argentina and CONICET.
authors.forename: M F
authors.initials: MF
authors.lastname: Martini
authors.affiliation: Centro de Investigaciones y Transferencia Santiago del Estero (CITSE), (CONICET-UNSE), Laboratorio de Biointerfases y Sistemas Biomiméticos, Laboratorios Centrales - Ala Norte, Ruta Nacional 9, Km 1125 - Villa El Zanjón, CP 4206 Santiago del Estero, Argentina.
authors.forename: A M
authors.initials: AM
authors.lastname: Bouchet
authors.affiliation: Centro de Investigaciones y Transferencia Santiago del Estero (CITSE), (CONICET-UNSE), Laboratorio de Biointerfases y Sistemas Biomiméticos, Laboratorios Centrales - Ala Norte, Ruta Nacional 9, Km 1125 - Villa El Zanjón, CP 4206 Santiago del Estero, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Hollmann
authors.affiliation: Centro de Investigaciones y Transferencia Santiago del Estero (CITSE), (CONICET-UNSE), Laboratorio de Biointerfases y Sistemas Biomiméticos, Laboratorios Centrales - Ala Norte, Ruta Nacional 9, Km 1125 - Villa El Zanjón, CP 4206 Santiago del Estero, Argentina.
authors.forename: M A
authors.initials: MA
authors.lastname: Frías
mesh_terms: D000818:Animals; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008567:Membranes, Artificial; D008956:Models, Chemical; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D008567:Membranes, Artificial; D010455:Peptides; D014867:Water
keywords: Aqueous interphases; Lipid membranes; Peptide interaction; Thermodynamic response; Water organization