title: Molecular typing of Treponema pallidum in the Czech Republic during 2011 to 2013: increased prevalence of identified genotypes and of isolates with macrolide resistance.
abstract: From January 2011 to December 2013, a total of 262 samples, from 188 patients suspected of having syphilis were tested for the presence of treponemal DNA by PCR amplification of five chromosomal loci, including the polA (TP0105), tmpC (TP0319), TP0136, TP0548, and 23S rRNA genes. Altogether, 146 samples from 103 patients were PCR positive for treponemal DNA. A set of 81 samples from 62 PCR-positive patients were typeable, and among them, nine different genotypes were identified. Compared to a previous study in the Czech Republic during 2004 to 2010, the number of genotypes detected among syphilis patients in a particular year increased to six in both 2012 and 2013, although they were not the same six. The proportion of macrolide-resistant clinical isolates in this 3-year study was 66.7%.
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Linda
authors.initials: L
authors.lastname: Grillová
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Pĕtrošová
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Mikalová
authors.affiliation: Department of Dermatovenereology, St. Anne's Faculty Hospital Brno, Brno, Czech Republic.
authors.forename: Radim
authors.initials: R
authors.lastname: Strnadel
authors.affiliation: Department of Dermatovenereology, St. Anne's Faculty Hospital Brno, Brno, Czech Republic.
authors.forename: Eliška
authors.initials: E
authors.lastname: Dastychová
authors.affiliation: Department of Dermatology, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Kuklová
authors.affiliation: Department of Dermatology, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Kojanová
authors.affiliation: Institute of Medical Biochemistry and Laboratory Diagnostics of the General University Hospital and of the 1st Faculty of Medicine of Charles University in Prague, Prague, Czech Republic.
authors.forename: Miluše
authors.initials: M
authors.lastname: Kreidlová
authors.affiliation: Department of Dermatovenereology, 2nd Faculty of Medicine, Charles University in Prague, Bulovka Hospital, Prague, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Vaňousová
authors.affiliation: Department of Dermatovenereology, 2nd Faculty of Medicine, Charles University in Prague, Bulovka Hospital, Prague, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Hercogová
authors.affiliation: Outpatient STI Clinic Medicentrum, Prague, Czech Republic.
authors.forename: Přemysl
authors.initials: P
authors.lastname: Procházka
authors.affiliation: The National Reference Laboratory for Diagnostics of the Syphilis, National Institute for Public Health, Prague, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Zákoucká
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, St. Anne's Hospital and Masaryk University, Brno, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Krchňáková
authors.affiliation: Department of Dermatovenereology, St. Anne's Faculty Hospital Brno, Brno, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Vašků
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic dsmajs@med.muni.cz.
authors.forename: David
authors.initials: D
authors.lastname: Šmajs
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018153:Czech Republic / Q000453:epidemiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005260:Female; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D018942:Macrolides / Q000494:pharmacology*; D008297:Male; D058889:Molecular Typing; D015995:Prevalence; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013587:Syphilis / Q000453:epidemiology / Q000382:microbiology*; D014210:Treponema pallidum / Q000145:classification* / Q000187:drug effects / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides; D012338:RNA, Ribosomal, 23S
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