title: Construction of carbon nanotube based nanoarchitectures for selective impedimetric detection of cancer cells in whole blood.
abstract: A carbon nanotube (CNT) based nanoarchitecture is developed for rapid, sensitive and specific detection of cancer cells by using real time electrical impedance sensing. The sensor is constructed with carbon nanotube (CNT) multilayers and EpCAM (epithelial cell adhesion molecule) antibodies, which are assembled on an indium tin oxide (ITO) electrode surface. The binding of tumor cells to EpCAM antibodies causes increase of the electron-transfer resistance. The electrochemical impedance of the prepared biosensors is linear with the logarithm of concentration of the liver cancer cell line (HepG2) within the concentration range of 10 to 10(5) cells per mL. The detection limit for HepG2 cells is 5 cells per mL. The proposed impedimetric sensing devices allow for sensitive and specific detection of cancer cells in whole-blood samples without any sample pretreatment steps.
authors.affiliation: Key Laboratory of Microsystems and Microstructures Manufacturing, Ministry of Education, Harbin Institute of Technology, Harbin, 150080, China. shaoqinliu@hit.edu.cn shaoqinliu@hotmail.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Fanjiao
authors.initials: F
authors.lastname: Zhu
authors.forename: Wangxia
authors.initials: W
authors.lastname: Dan
authors.forename: Yu
authors.initials: Y
authors.lastname: Fu
authors.forename: Shaoqin
authors.initials: S
authors.lastname: Liu
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D015374:Biosensing Techniques; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods*; D015815:Cell Adhesion Molecules / Q000276:immunology; D017097:Electric Impedance; D004566:Electrodes; D000071858:Epithelial Cell Adhesion Molecule; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D018189:Immunomagnetic Separation; D018613:Microscopy, Confocal; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009360:Neoplastic Cells, Circulating / Q000737:chemistry* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D000906:Antibodies; D055910:Antibodies, Immobilized; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D005456:Fluorescent Dyes; D037742:Nanotubes, Carbon; D017971:Tin Compounds; C109984:indium tin oxide