title: Purification and culture of dorsal root ganglion neurons.
abstract: Dorsal root ganglion neurons (DRGs) are sensory neurons that reside in ganglions on the dorsal root of the spinal cord. Here we introduce a method for the acute, prospective purification and culture of DRGs from rodents in a serum-free, defined medium, in the absence of glial cells. This immunopanning-based method facilitates the study of DRG biology and function.
authors.affiliation: Department of Neurobiology, School of Medicine, Stanford University, Stanford, California 94305.
authors.forename: J Bradley
authors.initials: JB
authors.lastname: Zuchero
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D016895:Culture Media, Serum-Free / Q000737:chemistry; D005727:Ganglia, Spinal / Q000166:cytology*; D009474:Neurons / Q000502:physiology*; D012377:Rodentia
chemical_list: D016895:Culture Media, Serum-Free
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