title: Hymenolepis diminuta: analysis of the expression of Toll-like receptor genes and protein (TLR3 and TLR9) in the small and large intestines of rats.
abstract: Toll-like receptors (TLRs) play a fundamental role in the rapid activation of innate immune responses to a variety of pathogen-associated molecular patterns (PAMPs). In a previous study we observed an increase in the level of expression of TLR2 and TLR4 mRNA in the jejunum and colon during experimental hymenolepidosis in rats. In this study, we performed a quantitative real-time polymerase chain reaction (qRT-PCR), Western blot analysis and immunohistochemical staining of TLR3 and TLR9 receptors during experimental hymenolepidosis in rats. The levels of mRNA and protein expression of TLR3 and TLR9 in the jejunum had increased at 16 days post Hymenolepis diminuta infection (dpi) in the case of TLR3 and at 16 and 25 dpi in the case of TLR9. In the colon the expression of TLR3 and TLR9 had increased at 16, 25 and 40 dpi. The results of the immunohistochemical reactions showed that H. diminuta infected rats (16, 25, 40 and 60 dpi) exhibited changes in TLR3 and TLR9 localization and intensity in the epithelial cells of the jejunum and colon. The changes in the level of TLR3 and TLR9 expression may confirm involvement of the innate immune system in the pathomechanism of hymenolepidosis.
authors.affiliation: Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland. Electronic address: kodan@pum.edu.pl.
authors.forename: Danuta I
authors.initials: DI
authors.lastname: Kosik-Bogacka
authors.affiliation: Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701 Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wojtkowiak-Giera
authors.affiliation: Department of Histology and Embryology, Pomeranian Medical University, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kolasa
authors.affiliation: Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Baranowska-Bosiacka
authors.affiliation: Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Lanocha
authors.affiliation: Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701 Poznan, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Wandurska-Nowak
authors.affiliation: Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Gutowska
authors.initials: G
authors.lastname: Izabela
authors.affiliation: Department of General Biology and Parasitology, Medical University of Warsaw, Chałubińskiego 5, 02-004 Warsaw, Poland.
authors.forename: Ruslan
authors.initials: R
authors.lastname: Salamatin
authors.affiliation: Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Święcickiego 6, 60-781 Poznan, Poland.
authors.forename: Paweł P
authors.initials: PP
authors.lastname: Jagodzinski
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005786:Gene Expression Regulation; D006925:Hymenolepiasis / Q000378:metabolism*; D048190:Hymenolepis diminuta / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D007420:Intestine, Large / Q000378:metabolism / Q000469:parasitology; D007421:Intestine, Small / Q000378:metabolism / Q000469:parasitology; D008297:Male; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D051196:Toll-Like Receptor 3 / Q000235:genetics / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000235:genetics / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C530472:TLR3 protein, rat; C489640:Tlr9 protein, rat; D051196:Toll-Like Receptor 3; D051217:Toll-Like Receptor 9; D051193:Toll-Like Receptors
keywords: Colon; Hymenolepis diminuta; Jejunum; Rats; Toll-like receptors