title: De novo transcriptomes of olfactory epithelium reveal the genes and pathways for spawning migration in japanese grenadier anchovy (Coilia nasus).
abstract: BACKGROUND
  Coilia nasus (Japanese grenadier anchovy) undergoes spawning migration from the ocean to fresh water inland. Previous studies have suggested that anadromous fish use olfactory cues to perform successful migration to spawn. However, limited genomic information is available for C. nasus. To understand the molecular mechanisms of spawning migration, it is essential to identify the genes and pathways involved in the migratory behavior of C. nasus.
  
  
  RESULTS
  Using de novo transcriptome sequencing and assembly, we constructed two transcriptomes of the olfactory epithelium from wild anadromous and non-anadromous C. nasus. Over 178 million high-quality clean reads were generated using Illumina sequencing technology and assembled into 176,510 unigenes (mean length: 843 bp). About 51% (89,456) of the unigenes were functionally annotated using protein databases. Gene ontology analysis of the transcriptomes indicated gene enrichment not only in signal detection and transduction, but also in regulation and enzymatic activity. The potential genes and pathways involved in the migratory behavior were identified. In addition, simple sequence repeats and single nucleotide polymorphisms were analyzed to identify potential molecular markers.
  
  
  CONCLUSIONS
  We, for the first time, obtained high-quality de novo transcriptomes of C. nasus using a high-throughput sequencing approach. Our study lays the foundation for further investigation of C. nasus spawning migration and genome evolution.
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
authors.forename: Guoli
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Liangjiang
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Tang
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
authors.forename: Jinquan
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D029941:Fish Proteins / Q000235:genetics*; D048388:Gadiformes / Q000235:genetics* / Q000502:physiology*; D009831:Olfactory Mucosa / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D029941:Fish Proteins
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