title: Chitosan nanoparticles as non-viral gene delivery systems: determination of loading efficiency.
abstract: Chitosan has been studied for use in particle delivery systems for therapeutic purposes, since one of its most important applications is as a non-viral vector in gene therapy. Due to its positive charge, it is capable of forming DNA complexes (polyplexes) obtained through several methods and with the property of protecting nucleic acids. Two methods for obtaining the nanoparticles of chitosan-nucleic acids are reported in this study: simple complexation (of depolymerized chitosan or of different chitosan salts with plasmid) and ionic gelation (by adsorption of plasmid in the nanoparticles or by encapsulation of plasmid into nanoparticles). The determination of the loading efficiency of chitosan nanoparticles with the plasmid is carried out by electrophoretic mobility of the samples on agarose gel. Furthermore, the nanoparticles have been characterized according to their morphology, size and surface charge using AFM, TEM, laser diffraction and dynamic light scattering techniques. The polyplexes obtained have been found to be spherical and nanometric in size (between 100-230nm) with a zeta potential between 37 and 48mV. Positive results have been obtained by agarose gel electrophoresis for all studied cases: a concentration of between 20 and 30μg/mL of chitosan salts is required while for the remaining chitosan samples studied, 100% loading efficiency does not occur until a concentration equal to 100μg/mL (regardless of previous depolymerisation and the method performed). Chitosan-plasmid nanocapsules have been obtained at the polymer concentrations worked with (between 0.025 and 0.2%).
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Technology Department, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: carolinacarrillo@ub.edu.
authors.forename: Carolina
authors.initials: C
authors.lastname: Carrillo
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Technology Department, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: jmsune@ub.edu.
authors.forename: Josep Maria
authors.initials: JM
authors.lastname: Suñé
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Technology Department, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: perezlo@ub.edu.
authors.forename: Pilar
authors.initials: P
authors.lastname: Pérez-Lozano
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Technology Department, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: encarnagarcia@ub.edu.
authors.forename: Encarna
authors.initials: E
authors.lastname: García-Montoya
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Technology Department, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: rsarraar@hotmail.es.
authors.forename: Rocío
authors.initials: R
authors.lastname: Sarrate
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Technology Department, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: afabregas@ub.edu.
authors.forename: Anna
authors.initials: A
authors.lastname: Fàbregas
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Technology Department, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: minarromontse@ub.edu.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Miñarro
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Technology Department, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: jrtico@ub.edu.
authors.forename: Josep Ramon
authors.initials: JR
authors.lastname: Ticó
mesh_terms: D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D018014:Gene Transfer Techniques / Q000639:trends; D015316:Genetic Therapy / Q000379:methods* / Q000639:trends; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D048271:Chitosan
keywords: Chitosan; Gene therapy; Nanoparticles; Non-viral vector; Particle delivery systems; Polyplexes