title: Mode of swine hepatitis E virus infection and replication in primary human hepatocytes.
abstract: The aim of this study was to investigate the infection and replication of swine-derived hepatitis E virus (HEV) in primary cultured human hepatocytes (PHCs). Hepatocytes were cultured from the resected normal livers of patients with metastatic tumours. These cultured hepatocytes were infected with swine-derived genotype 3 or 4 HEV. Viral replication was monitored using reverse transcriptase-quantitative PCR. The amount of HEV RNA increased in the culture media and cells following infection. Immunofluorescence staining implied that the spread of HEV infection in hepatocytes was attributed mainly to cell-to-cell transmission via the cell membrane. The sequences of the inoculated and propagated HEV were determined to examine whether sequence variation occurred during infection. Sequence analysis showed that there were no differences between inoculated and propagated HEV, demonstrating that in vitro infection and replication of swine HEV in PHCs occurred without sequence variation.
authors.affiliation: Division of Gastroenterological and Hepatobiliary Surgery and Organ Transplantation, University of Tsukuba, Tsukuba, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Oshiro
authors.affiliation: Animal Genome Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yasue
authors.affiliation: Division of Gastroenterological and Hepatobiliary Surgery and Organ Transplantation, University of Tsukuba, Tsukuba, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Japan Blood Products Organization, Kobe, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Hattori
authors.affiliation: Japan Blood Products Organization, Kobe, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Ideno
authors.affiliation: Japan Blood Products Organization, Kobe, Japan.
authors.forename: Takeru
authors.initials: T
authors.lastname: Urayama
authors.affiliation: Division of Gastroenterological and Hepatobiliary Surgery and Organ Transplantation, University of Tsukuba, Tsukuba, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Chiba
authors.affiliation: Department of Infection Biology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Suguru
authors.initials: S
authors.lastname: Osari
authors.affiliation: Kawasaki Medical School, Okayama, Japan.
authors.forename: Tadasuke
authors.initials: T
authors.lastname: Naito
authors.affiliation: Department of Infection Biology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takeuchi
authors.affiliation: Department of Infection Biology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nagata
authors.affiliation: Division of Gastroenterological and Hepatobiliary Surgery and Organ Transplantation, University of Tsukuba, Tsukuba, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ohkohchi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016679:Genome, Viral; D005838:Genotype; D016751:Hepatitis E / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology*; D006801:Humans; D013552:Swine; D013553:Swine Diseases / Q000821:virology*