title: Longitudinal assessment of mouse renal injury using high-resolution anatomic and magnetization transfer MR imaging.
abstract: The purpose of this study is to evaluate the utility of high-resolution non-invasive endogenous high-field MRI methods for the longitudinal structural and quantitative assessments of mouse kidney disease using the model of unilateral ureter obstruction (UUO). T1-weighted, T2-weighted and magnetization transfer (MT) imaging protocols were optimized to improve the regional contrast in mouse kidney. Conventional T1 and T2 weighted images were collected in UUO mice on day 0 (~3h), day 1, day 3 and day 6 after injury, on a 7 T small animal MRI system. Cortical and medullary thickness, corticomedullary contrast and Magnetization Transfer Ratio (MTR) were assessed longitudinally. Masson trichrome staining was used to histologically assess changes in tissue microstructure. Over the course of UUO progression there were significant (p<0.05) changes in thickness of cortex and outer medulla, and regional changes in T2 signal intensity and MTR values. Histological changes included tubular cell death, tubular dilation, urine retention, and interstitial fibrosis, assessed by histology. The MRI measures of renal cortical and medullary atrophy, cortical-medullary differentiation and MTR changes provide an endogenous, non-invasive and quantitative evaluation of renal morphology and tissue composition during UUO progression.
authors.affiliation: Vanderbilt University Institute of Imaging Science; Departments of Radiology and Radiological Sciences.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Vanderbilt O'Brien Mouse Kidney Physiology and Disease Center.
authors.forename: Rosie
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Vanderbilt O'Brien Mouse Kidney Physiology and Disease Center.
authors.forename: Keiko
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Vanderbilt University Institute of Imaging Science; Departments of Radiology and Radiological Sciences; Biomedical Engineering; Cancer Biology.
authors.forename: John
authors.initials: J
authors.lastname: Gore
authors.affiliation: Vanderbilt O'Brien Mouse Kidney Physiology and Disease Center.
authors.forename: Raymond C
authors.initials: RC
authors.lastname: Harris
authors.affiliation: Cancer Biology; Vanderbilt O'Brien Mouse Kidney Physiology and Disease Center. Electronic address: takamune.takahashi@vanderbilt.edu.
authors.forename: Takamune
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Vanderbilt University Institute of Imaging Science; Departments of Radiology and Radiological Sciences; Biomedical Engineering; Cancer Biology. Electronic address: chad.quarles@vanderbilt.edu.
authors.forename: C Chad
authors.initials: CC
authors.lastname: Quarles
mesh_terms: D000818:Animals; D003287:Contrast Media; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007668:Kidney / Q000473:pathology*; D007674:Kidney Diseases / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice
chemical_list: D003287:Contrast Media
keywords: Cortical thickness; Cortical–medullary contrast; MRI; Magnetization transfer (MT); Renal models of disease; Unilateral ureteral obstruction
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