title: MIRA analysis of RARβ2 gene methylation in DNA circulating in the blood in lung cancer.
abstract: Analysis of DNA epigenetic mutations in the blood circulating DNA is a prospective trend for creation of noninvasive methods for the diagnosis and treatment efficiency monitoring in cancer. The methylation status of target genes in circulating DNA was evaluated by methods based on preliminary bisulfite conversion of DNA. We used a different approach based on selection of hypermethylated sequences of circulating DNA by means of DNA-methyl-binding protein (methylated CpG island recovery assay, MIRA). Methylation was evaluated for RARβ2 tumor suppression gene in circulating DNA in lung cancer and a trend was detected to higher methylation of this gene in the patients in comparison with healthy donors.
authors.affiliation: Institute of Organic Biochemistry, the Russian Academy of Sciences, Moscow, Russia.
authors.forename: K Yu
authors.initials: KY
authors.lastname: Kapitskaya
authors.forename: T L
authors.initials: TL
authors.lastname: Azhikina
authors.forename: A A
authors.initials: AA
authors.lastname: Ponomaryova
authors.forename: N V
authors.initials: NV
authors.lastname: Cherdyntseva
authors.forename: V V
authors.initials: VV
authors.lastname: Vlasov
authors.forename: P P
authors.initials: PP
authors.lastname: Laktionov
authors.forename: E Yu
authors.initials: EY
authors.lastname: Rykova
mesh_terms: D000368:Aged; D001681:Biological Assay; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D018899:CpG Islands; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000097:blood* / Q000235:genetics; D044127:Epigenesis, Genetic; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018168:Receptors, Retinoic Acid / Q000097:blood* / Q000235:genetics; D013447:Sulfites / Q000737:chemistry
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D018168:Receptors, Retinoic Acid; D013447:Sulfites; C540966:retinoic acid receptor, beta2, human; C042345:hydrogen sulfite