title: Postnatal expression patterns and polymorphism analysis of the bovine myocyte enhancer factor 2C (Mef2C) gene.
abstract: The aim of this study was to analyze the level of expression of the Mef2C gene in the developing bovine longissimus dorsi (LD) muscle (at 6, 9 and 12months of age) and to evaluate differences in expression among Polish Holstein-Friesian (HO) and Limousine (LM) bulls. Moreover, the expression patterns of Mef2C in different tissues were determined. The results showed that Mef2C mRNA was expressed at a high level in adult skeletal and cardiac muscles. Moreover, Mef2C expression was markedly lower in the semitendinosus (ST) than in the gluteus medius (GM) and LD muscles. A relatively higher Mef2C mRNA and MEF2C protein level was estimated in the muscles of HO bulls at the age of 12months in comparison with its lower expression in LM bulls. Furthermore, we found that the Mef2C promoter variant (GU211004:g.-1606C>T) does not affect the level of mRNA in the LD and ST muscles of 12-month-old HO bulls.
authors.affiliation: Department of Molecular Cytogenetics, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzębiec, Poland. Electronic address: e.kubiak@ighz.pl.
authors.forename: E
authors.initials: E
authors.lastname: Juszczuk-Kubiak
authors.affiliation: Department of Molecular Cytogenetics, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzębiec, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Wicińska
authors.affiliation: Department of Molecular Biology, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzębiec, Poland.
authors.forename: R R
authors.initials: RR
authors.lastname: Starzyński
mesh_terms: D000367:Age Factors; D000375:Aging; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D064326:MEF2 Transcription Factors / Q000235:genetics*; D008297:Male; D018482:Muscle, Skeletal; D009206:Myocardium; D011110:Polymorphism, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome / Q000235:genetics*
chemical_list: D064326:MEF2 Transcription Factors; D012333:RNA, Messenger
keywords: Cattle; Gene expression; Mef2C; Myogenesis; Skeletal muscle