title: [Metabonomic study on protective effect of xiaoyao powder for acute hepatic injury in rats].
abstract: OBJECTIVE
  To study the metabonomic for acute hepatic injury in rats and the hepato-protective effect of Xiaoyao Powder in rats.
  
  
  METHODS
  Male rats were treated with Xiaoyao Powder decoction through intragastric for 7 days. 0.2% CCl4 peanut oil solution were given by intraperitoneal injection in one hour after 7 days. The rats were fasted for 16 hours, but water was given. Hepatic tissues were used for histopathological examination. Pre-column derivatization-GC-MS was used to detect the changes in rats serum metabolites. Mass spectrometry analysis, PCA and other technolgy technical were used to analyze the differences among their metabolites.
  
  
  RESULTS
  Compared with normal group, model group rats hepatic tissue was destroyed obviously and nodular regenerative hyperplasia of hepatic cells were clear. Alanine, glycine, serine, threonine, aminomalonic acid, malic acid, aspartic acid, ornithine, lysine, inositol and glutamine were significantly increased in serum. All the indicators were increased after treated with Xiaoyao Powder.
  
  
  CONCLUSIONS
  Xiaoyao Powder has curative effect on acute hepatic injury, and its mechanism may be related to adjusting aliphatic acid metabolism and promoting amino acids generated function.
authors.forename: Fang
authors.initials: F
authors.lastname: Geng
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Heng
authors.initials: H
authors.lastname: Fang
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Liu
mesh_terms: D000596:Amino Acids / Q000097:blood*; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000009:adverse effects; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D055442:Metabolome; D055432:Metabolomics; D010946:Plants, Medicinal / Q000737:chemistry; D011208:Powders; D025341:Principal Component Analysis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; D011208:Powders; C516449:xiaoyao; D002251:Carbon Tetrachloride