title: Enhancement of L-cysteine production by disruption of yciW in Escherichia coli.
abstract: Using in silico analysis, the yciW gene of Escherichia coli was identified as a novel L-cysteine regulon that may be regulated by the transcriptional activator CysB for sulfur metabolic genes. We found that overexpression of yciW conferred tolerance to L-cysteine, but disruption of yciW increased L-cysteine production in E. coli.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kawano
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan. Electronic address: iohtsu@bs.naist.jp.
authors.forename: Iwao
authors.initials: I
authors.lastname: Ohtsu
authors.affiliation: Research Institute for Bioscience Products and Fine Chemicals, Ajinomoto Co., 1-1 Suzuki-cho, Kawasaki, Kanagawa 210-8681, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takumi
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Tamakoshi
authors.affiliation: Research Institute for Bioscience Products and Fine Chemicals, Ajinomoto Co., 1-1 Suzuki-cho, Kawasaki, Kanagawa 210-8681, Japan.
authors.forename: Gen
authors.initials: G
authors.lastname: Nonaka
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Funahashi
authors.affiliation: Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, Nagano 399-4511, Japan; JST, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ihara
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takagi
mesh_terms: D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000378:metabolism; D001665:Binding Sites; D003198:Computer Simulation; D003545:Cysteine / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D018085:Regulon / Q000235:genetics; D013455:Sulfur / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C062886:CysB protein, Bacteria; D015534:Trans-Activators; D013455:Sulfur; D003545:Cysteine
keywords: CysB-binding motif; Escherichia coli; In silico analysis; l-Cysteine fermentation; yciW gene