title: The effect of long-term supplementation with different dietary ω-6/ω-3 ratios on mineral content and ex vivo prostaglandin E2 release in bone of growing rabbits.
abstract: OBJECTIVE
  The aim of this research was to study the different long term effects of consumption of dietary oil sources with varying omega-6/omega-3 (ω-6/ω-3) polyunsaturated fatty acids (PUFAs) ratios on bone marrow fatty acid level, ex vivo prostaglandin E2 (PGE2) release, and mineral content of bone in rabbits.
  
  
  METHODS
  For this purpose, weaning and female New Zealand white rabbits were purchased and randomly divided into five groups and offered ad libitum diets containing 70 g/kg of added oil for 100 days. The dietary lipid treatments were formulated to provide the following ratios of ω-6/ω-3 fatty acids: 8.68 soy bean oil (SBO control), 21.75 sesame oil (SO), 0.39 fish oil (FO), 0.63 algae oil (DHA), and 0.68 algae oils (DHA/ARA). DHA and ARA are two types of marine microalgae of the genus Crypthecodinium cohnii.
  
  
  RESULTS
  The dietary treatments had significant effects on the bone marrow fatty acids of rabbits. Rabbits fed the FO diet, containing the highest ω-3 PUFA concentration, and those fed the SBO diet showed the highest ω-6 PUFA. On the other hand, a positive correlation was observed between Ex vivo PGE2 level and the ω-6/ω-3 dietary ratio. Significant effects of dietary treatment on femur Ca, P, Mg, and Zn contents were observed in both genders.
  
  
  CONCLUSIONS
  Findings of the current study clearly demonstrated that dietary PUFA, particularly ω-6/ω-3 and ARA/EPA ratios are important factors in determining bone marrow fatty acid profile, and this in turn determines the capacity of bone for synthesis of PGE2, thereby reducing bone resorption and improving bone mass during growth.
authors.affiliation: Department of Food Sciences and Nutrition, Faculty of Food Sciences and Agriculture, King Saud University, Saudi Arabia. P.O. Box 2460, Riyadh 11451 Saudi Arabia.
authors.forename: Doha Mustafa
authors.initials: DM
authors.lastname: Alnouri
authors.affiliation: Department of Food Sciences and Nutrition, Faculty of Food Sciences and Agriculture, King Saud University, Saudi Arabia. P.O. Box 2460, Riyadh 11451 Saudi Arabia. ; Department of Food Science and Nutrition, Faculty of Home Economics, Minufiya University, Egypt.
authors.forename: Mohamed Fekry Serag
authors.initials: MF
authors.lastname: El-Din
authors.affiliation: Department of Food Sciences and Nutrition, Faculty of Food Sciences and Agriculture, King Saud University, Saudi Arabia. P.O. Box 2460, Riyadh 11451 Saudi Arabia.
authors.forename: Abdulrhman Salih
authors.initials: AS
authors.lastname: Al-Khalifa
keywords: bone marrow; minerals; prostaglandin E2; rabbits; ω-6/ω-3 ratio
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