title: Solid-phase supports for the in situ assembly of quantum dot-FRET hybridization assays in channel microfluidics.
abstract: Semiconductor quantum dots (QDs) have long served as integral components in signal transduction modalities such as Förster resonance energy transfer (FRET). The majority of bioanalytical methods using QDs for FRET-based techniques simply monitor binding-induced conformational changes. In more recent work, QDs have been incorporated into solid-phase support systems, such as microfluidic chips, to serve as physical platforms in the development of functional biosensors and bioprobes. Herein, we describe a simple strategy for the transduction of nucleic acid hybridization that combines a novel design method based on FRET with an electrokinetically controlled microfluidic technology, and that offers further potential for amelioration of sample-handling issues and for simplification of dynamic stringency control.
authors.affiliation: Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Rd. North, Mississauga, ON, Canada, L5L 1C6.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Tavares
authors.forename: M Omair
authors.initials: MO
authors.lastname: Noor
authors.forename: Uvaraj
authors.initials: U
authors.lastname: Uddayasankar
authors.forename: Ulrich J
authors.initials: UJ
authors.lastname: Krull
authors.forename: Charles H
authors.initials: CH
authors.lastname: Vannoy
mesh_terms: D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D005898:Glass / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D001710:Biotin; D004247:DNA