title: Preparation of monolithic molecularly imprinted polymer sol-gel packed tips for high-throughput bioanalysis: extraction and quantification of L-tyrosine in human plasma and urine samples utilizing liquid chromatography and tandem mass spectrometry.
abstract: In situ monolithic molecularly imprinted polymer sol-gel packed tips (MMSTs) were prepared and evaluated for the extraction of lung cancer biomarker L-tyrosine (Tyr) from human plasma and urine samples. Several extraction parameters such as the conditioning, washing and elution solutions, pH and time were investigated. The enrichment factor (EF) and extraction recovery (ER) were studied. MMST showed good selectivity and a high extraction recovery, and MMST as a sorbent showed good stability and repeatability. The method validation showed good regression correlation coefficients for plasma and urine samples (R(2)≥0.996) within the concentration range of 5-1000 and 1-1000 nmol L(-1) in plasma and urine samples, respectively. The lower limits of quantification (LLOQ) in the plasma and urine samples were 5 and 1 nmol L(-1), respectively. The between-batch precision for Tyr in plasma ranged from 1.0 to 6.0%, and in urine it was from 1.0 to 7.0%. The results show that the developed method has more facility, stability, durability and repeatability compared with previous similar methods. To the best of our knowledge, this is the first study aimed at the selective separation of Tyr as a lung cancer biomarker by MMSTs from biological matrixes and detection by LC/MS/MS.
authors.affiliation: Department of Analytical Chemistry, Stockholm University, SE10691 Stockholm, Sweden.
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Moein
authors.affiliation: Department of Analytical Chemistry, Stockholm University, SE10691 Stockholm, Sweden.
authors.forename: Aziza
authors.initials: A
authors.lastname: El-Beqqali
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester M60 1QD, UK.
authors.forename: Abbi
authors.initials: A
authors.lastname: Abdel-Rehim
authors.affiliation: Department of Analytical Chemistry, Stockholm University, SE10691 Stockholm, Sweden.
authors.forename: Amin
authors.initials: A
authors.lastname: Jeppsson-Dadoun
authors.affiliation: Department of Analytical Chemistry, Stockholm University, SE10691 Stockholm, Sweden. Electronic address: mohamed.abdel-rehim@anchem.su.se.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdel-Rehim
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D057166:High-Throughput Screening Assays; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D054802:Molecular Imprinting / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014443:Tyrosine / Q000097:blood* / Q000302:isolation & purification / Q000652:urine*
chemical_list: D014443:Tyrosine
keywords: Liquid chromatography–tandem mass spectrometry; Monolithic molecularly imprinted polymer sol–gel in-tip; Plasma; Tyrosine; Urine