title: Functional characterization of Autographa californica multiple nucleopolyhedrovirus gp16 (ac130).
abstract: To investigate the function of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) gp16, multiple gp16-knockout and repair mutants were constructed and characterized. No obvious difference in productivity of budded virus, DNA synthesis, late gene expression and morphogenesis was observed between gp16-knockout and repair viruses, but gp16 deletion resulted in six hours of lengthening in ST50 to the third instar Spodoptera exigua larvae in bioassays. GP16 was fractionated mainly in the light membrane fraction, by subcellular fractionation. A GP16-EGFP fusion protein was predominantly localized close around the nuclear membrane in infected cells, being coincident with formation of the vesicles associated with the nuclear membrane, which hosted nucleocapsids released from the nucleus. These data suggest that gp16 is not required for viral replication, but may be involved in membrane trafficking associated with the envelopment/de-envelopment of budded viruses when they cross over the nuclear membrane and pass through cytoplasm.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Huang
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China.
authors.forename: Duo-Duo
authors.initials: DD
authors.lastname: Qian
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China. Electronic address: lilulin@mail.ccnu.edu.cn.
authors.forename: Lu-Lin
authors.initials: LL
authors.lastname: Li
mesh_terms: D000818:Animals; D009685:Nuclear Envelope / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D018411:Spodoptera / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D014764:Viral Proteins
keywords: Autographa californica multiple nucleopolyhedrovirus; ac130; gp16; gp16 knockout