title: Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice.
abstract: BACKGROUND
  The presence of oligoclonal IgM bands (OCMB) in cerebrospinal fluid (CSF) is an unfavourable prognostic marker in multiple sclerosis. There is no commercial test to investigate OCMB status. However, a sensitive and specific isoelectrofocusing (IEF) and western blot method was described. We aimed to study the inter-centre reproducibility of this technique, a necessary condition for a reliable test to be incorporated into clinical practice.
  
  
  METHODS
  The presence of OCMB was analysed by IEF and western blot with prior reduction of pentameric IgM. We assayed the reproducibility of this test in a blinded multicentre study performed in 13 university hospitals. Paired-CSF and serum samples from 52 neurological patients were assayed at every centre.
  
  
  RESULTS
  Global analysis rendered a concordance of 89.8% with a kappa value of 0.71.
  
  
  CONCLUSIONS
  These data indicate that OCMB detection by means of IEF and western blot with IgM reduction shows a good interlaboratory reproducibility and thus can be used in daily clinical setting.
authors.affiliation: Department of Immunology, Hospital Universitario Ramón y Cajal, Ctra de Colmenar Viejo km 9.100, 28034 Madrid, IRYCIS, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: M
authors.initials: M
authors.lastname: Espiño
authors.affiliation: Department of Biostatistics, Hospital Universitario Ramón y Cajal, Ctra de Colmenar Viejo km 9.100, 28034 Madrid, IRYCIS, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Abraira
authors.affiliation: Department of Neurology, Hospital Clínico San Carlos, Calle Profesor Martín Lagos, s/n, 28040 Madrid, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: R
authors.initials: R
authors.lastname: Arroyo
authors.affiliation: Department of Neurology, Hospital Universitari de Bellvitge, Av. Granvia s/n, Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Bau
authors.affiliation: Department of Immunology, Hospital San Pedro de Alcántara, Avenida Pablo Naranjo s/n, 10003 Cáceres, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Cámara
authors.affiliation: Department of Neurology, Hospital Universitario Puerta de Hierro, Calle Manuel de Falla, 1, 28222 Majadahonda, Madrid, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: L
authors.initials: L
authors.lastname: Campos-Ruiz
authors.affiliation: Department of Neurology, Hospital Universitari La Fe, Avinguda Fernando Abril Martorell, 46026 Valencia, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: B
authors.initials: B
authors.lastname: Casanova
authors.affiliation: Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya, Vall d'Hebron Institut de Recerca, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Passeig Vall d'Hebron, 119-129, 08035 Barcelona, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: C
authors.initials: C
authors.lastname: Espejo
authors.affiliation: Department of Neurology, Hospital Regional Universitario, Avda Carlos Haya, s/n, 29010 Málaga, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: O
authors.initials: O
authors.lastname: Fernández
authors.affiliation: Department of Neurology, Hospital Universitario Puerta de Hierro, Calle Manuel de Falla, 1, 28222 Majadahonda, Madrid, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: A
authors.initials: A
authors.lastname: García-Merino
authors.affiliation: Department of Neurology, Hospital Universitario Virgen Macarena, Avd. Dr. Fedriani, 3, 41007 Sevilla, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: M I
authors.initials: MI
authors.lastname: García-Sánchez
authors.affiliation: Department of Neurology, Hospital San Pedro de Alcántara, Avenida Pablo Naranjo s/n, 10003 Cáceres, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Gómez
authors.affiliation: Red Española de Esclerosis Múltiple (REEM), Spain; Immunology, Hospital Regional Universitario, Avda Carlos Haya, s/n, 29010 Málaga, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Gosis
authors.affiliation: Department of Neurology, Hospital Universitario Virgen Macarena, Avd. Dr. Fedriani, 3, 41007 Sevilla, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: G
authors.initials: G
authors.lastname: Izquierdo
authors.affiliation: Department of Neurology, Hospital Universitario Virgen de la Arrixaca, Ctra. Madrid-Cartagena, s/n, 30120 El Palmar, Murcia, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: J
authors.initials: J
authors.lastname: Meca
authors.affiliation: Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya, Vall d'Hebron Institut de Recerca, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Passeig Vall d'Hebron, 119-129, 08035 Barcelona, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: X
authors.initials: X
authors.lastname: Montalban
authors.affiliation: Immunology, Hospital Universitari de Bellvitge, Av. Granvia s/n, Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Morandeira
authors.affiliation: Department of Neurology, Hospital Universitario Donostia, P° Dr. Beguiristain, 107-111, 20014 San Sebastián, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: J
authors.initials: J
authors.lastname: Olascoaga
authors.affiliation: Red Española de Esclerosis Múltiple (REEM), Spain; Department of Neurology, Hospital Universitario Donostia, P° Dr. Beguiristain, 107-111, 20014 San Sebastián, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Prada
authors.affiliation: Unitat de Neuroimmunologia i Esclerosi Múltiple, Hospital Universitari Dr. Josep Trueta, Institut d'Investigació Biomèdica de Girona (IDIBGI), Avenida França, s/n, 17007 Girona, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: E
authors.initials: E
authors.lastname: Quintana
authors.affiliation: Unitat de Neuroimmunologia i Esclerosi Múltiple, Hospital Universitari Dr. Josep Trueta, Institut d'Investigació Biomèdica de Girona (IDIBGI), Avenida França, s/n, 17007 Girona, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: Ll
authors.initials: L
authors.lastname: Ramió-Torrentà
authors.affiliation: Department of Neurology, Hospital Universitario Basurto, Av de Montevideo, 18, 48013 Bilbao, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez-Antigüedad
authors.affiliation: Immunology, Hospital Universitario Virgen de la Arrixaca, Ctra. Madrid-Cartagena, s/n, 30120 El Palmar, Murcia, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: G
authors.initials: G
authors.lastname: Salgado
authors.affiliation: Department of Neurology, Hospital Clínico San Carlos, Calle Profesor Martín Lagos, s/n, 28040 Madrid, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Santiago
authors.affiliation: Department of Neurology, Hospital Universitario Basurto, Av de Montevideo, 18, 48013 Bilbao, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: E
authors.initials: E
authors.lastname: Sarasola
authors.affiliation: Department of Neurology, Hospital Universitari La Fe, Avinguda Fernando Abril Martorell, 46026 Valencia, Spain; Red Española de Esclerosis Múltiple (REEM), Spain.
authors.forename: M
authors.initials: M
authors.lastname: Simó-Castelló
authors.affiliation: Red Española de Esclerosis Múltiple (REEM), Spain; Department of Neurology, Hospital Universitario Ramón y Cajal, Ctra de Colmenar Viejo km 9.100, 28034 Madrid, IRYCIS, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Alvarez-Cermeño
authors.affiliation: Department of Immunology, Hospital Universitario Ramón y Cajal, Ctra de Colmenar Viejo km 9.100, 28034 Madrid, IRYCIS, Spain; Red Española de Esclerosis Múltiple (REEM), Spain. Electronic address: luisamaria.villar@salud.madrid.org.
authors.forename: L M
authors.initials: LM
authors.lastname: Villar
mesh_terms: D015153:Blotting, Western; D006801:Humans; D007075:Immunoglobulin M / Q000134:cerebrospinal fluid*; D057230:Limit of Detection; D015203:Reproducibility of Results; D013030:Spain
chemical_list: D007075:Immunoglobulin M
keywords: Biomarker; Immunoglobulin IgM; Multiple sclerosis; Oligoclonal bands