title: Antigenicity and immunogenicity of fused B-subunit of heat labile toxin of Escherichia coli and colonization factor antigen I polyepitopes.
abstract: Linear B-cell epitopes ((93)AKEFEAAAL(101) and (66)PQLTDVLN(73)) of CfaB were genetically fused to ltb-(gly)5-cfaB(1-25). Sera of rabbits immunized with fusion proteins reacted strongly with solid-phase bound ETEC bacteria bearing CFA/I fimbriae. Sera failed to agglutinate or inhibit hemagglutination promoted by CFA/I-positive strain which may be due to solvent inaccessibility of epitope residues on intact fimbriae.
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran. Electronic address: nstrn_savar@yahoo.com.
authors.forename: Nastaran Sadat
authors.initials: NS
authors.lastname: Savar
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Dashti
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Darzi Eslam
authors.affiliation: Department of Pharmaceutical Biotechnology, and Bioinformatics Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jahanian-Najafabadi
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran. Electronic address: anisjafari@yahoo.com.
authors.forename: Anis
authors.initials: A
authors.lastname: Jafari
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology*; D036781:Fimbriae Proteins / Q000235:genetics / Q000276:immunology*; D006386:Hemagglutination Tests; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D000939:Epitopes; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; C022924:colonization factor antigens; D036781:Fimbriae Proteins; C027538:heat-labile enterotoxin, E coli
keywords: Colonization factor antigen; Enterotoxigenic Escherichia coli; Fusion protein; Heat-labile enterotoxin B subunit; Polyepitopes