title: Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy.
abstract: The myelin sheath plays an important role as the axon in the functioning of the neural system, and myelin degradation is a hallmark pathology of multiple sclerosis and spinal cord injury. Electron microscopy, fluorescent microscopy, and magnetic resonance imaging are three major techniques used for myelin visualization. However, microscopic observation of myelin in living organisms remains a challenge. Using a newly developed stimulated Raman scattering microscopy approach, we report noninvasive, label-free, real-time in vivo imaging of myelination by a single-Schwann cell, maturation of a single node of Ranvier, and myelin degradation in the transparent body of the Xenopus laevis tadpole.
authors.affiliation: University of Science and Technology of China, Hefei National Laboratory for Physical Sciences at Microscale, and the School of Life Sciences, Hefei 230027, China.
authors.forename: Chun-Rui
authors.initials: CR
authors.lastname: Hu
authors.affiliation: Purdue University, Department of Chemistry, West Lafayette, Indiana 47907, United States.
authors.forename: Delong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Purdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907, United States.
authors.forename: Mikhail N
authors.initials: MN
authors.lastname: Slipchenko
authors.affiliation: Purdue University, Department of Chemistry, West Lafayette, Indiana 47907, United StatescPurdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907, United States.
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Cheng
authors.affiliation: University of Science and Technology of China, Hefei National Laboratory for Physical Sciences at Microscale, and the School of Life Sciences, Hefei 230027, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
mesh_terms: D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D009186:Myelin Sheath / Q000648:ultrastructure*; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D014982:Xenopus laevis
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