title: Temporal changes in neutral endopeptidase/CD10 immunoexpression in the cyclic and early pregnant canine endometrium.
abstract: CD10 is a multifunctional transmembrane neutral endopeptidase (NEP) that is considered to be a reliable marker of ectopic human endometrial stroma. Available information on NEP/CD10 protein expression in animal endometria is scarce. This study focused on the immunolocalization of NEP/CD10 in the canine uterus and on its temporal changes during the estrous cycle and early pregnancy (Days 11 to 23 post-LH surge) in healthy females. NEP/CD10 expression was found in the canine endometrial stroma in all stages of the estrous cycle, showing cyclic differences both in intensity and in distribution pattern. A small population of negative stromal cells in subsurface position was also observed. This population shared some morphological characteristics with the human predecidual cells, which became positive in progesterone-associated stages of the cycle. In addition, positive immunolabeling was also observed in canine myometrial stroma. In early pregnancy, the basal glandular epithelia and the syncytium cords remained negative to this marker contrasting with the trophoblast and the lacunar epithelium. A weak to moderate intensity of immunolabeling was observed in the decidual cells, whereas stromal immunolabeling was more intense at the delimitation of the syncytium cords. In conclusion, CD10 is consistently expressed in the canine endometrial stroma and myometrium but not in the endometrial epithelia. The characteristic pattern seen in early pregnancy also suggests a role for this molecule in the process of embryo invasion at implantation.
authors.affiliation: Animal and Veterinary Research Centre (CECAV), Universidade de Trás-os-Montes e Alto Douro, UTAD, Vila Real, Portugal. Electronic address: rtpayan@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Payan-Carreira
authors.affiliation: Animal and Veterinary Research Centre (CECAV), Universidade de Trás-os-Montes e Alto Douro, UTAD, Vila Real, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Santos
authors.affiliation: Animal and Veterinary Research Centre (CECAV), Universidade de Trás-os-Montes e Alto Douro, UTAD, Vila Real, Portugal; Department of Veterinary Medicine, University School Vasco da Gama, Coimbra, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Miranda
authors.affiliation: Department of Veterinary Medicine, University School Vasco da Gama, Coimbra, Portugal; Strategic Unit for Biotechnology and Genetic Resources, National Institute of Agrarian and Veterinary Research (INIAV)-Pole at Quinta da Fonte Boa, Vale de Santarém, Portugal.
authors.forename: R M L N
authors.initials: RM
authors.lastname: Pereira
authors.affiliation: Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Universidade de Trás-os-Montes e Alto Douro, UTAD, Vila Real, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Santos
authors.affiliation: Animal and Veterinary Research Centre (CECAV), Universidade de Trás-os-Montes e Alto Douro, UTAD, Vila Real, Portugal.
authors.forename: M A
authors.initials: MA
authors.lastname: Pires
mesh_terms: D000818:Animals; D004285:Dogs / Q000502:physiology*; D030762:Estrous Cycle / Q000502:physiology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015260:Neprilysin / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal; D013997:Time Factors
chemical_list: D015260:Neprilysin
keywords: Early pregnancy; Endometrial stroma; Estrous cycle; Female dog; Immunohistochemistry; Neutral endopeptidase