title: Evaluation of the anti-inflammatory and antinociceptive effects of the essential oil from leaves of Xylopia laevigata in experimental models.
abstract: Xylopia laevigata (Annonaceae) is a medicinal plant used in folk medicine to treat pain and inflammation. Thus, we investigated the possible antioxidant, antinociceptive, and anti-inflammatory effects of X. laevigata leaf essential oil (EOX) in animal models. Our EOX sample showed the presence of γ-muurolene (17.78%), δ-cadinene (12.23%), bicyclogermacrene (7.77%), and α-copaene (7.17%) as main compounds. EOX presented a strong antioxidant potential according to the DPPH, TBARS, and nitrite production tests. Additionally, pretreatment with EOX, in mice, also significantly produced (P < 0.05 or P < 0.001) antinociceptive effect by reduction of nociceptive behavior (in formalin and writhing tests). The EOX showed c-Fos label in the olfactory bulb, piriform cortex, and periaqueductal gray. Acute administration of EOX exhibited a significant (P < 0.01 or P < 0.001) anti-inflammatory profile in the carrageenan-induced peritonitis and by the carrageenan-induced hindpaw edema tests in mice. Our results provide evidence for the use of X. laevigata by traditional medicine practitioners in the management of pain and inflammatory disorders.
authors.affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.
authors.forename: João Carlos C
authors.initials: JC
authors.lastname: Queiroz
authors.affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.
authors.forename: Angelo R
authors.initials: AR
authors.lastname: Antoniolli
authors.affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.
authors.forename: Lucindo J
authors.initials: LJ
authors.lastname: Quintans-Júnior
authors.affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.
authors.forename: Renan G
authors.initials: RG
authors.lastname: Brito
authors.affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.
authors.forename: Rosana S S
authors.initials: RS
authors.lastname: Barreto
authors.affiliation: Departamento de Química, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.
authors.forename: Emmanoel V
authors.initials: EV
authors.lastname: Costa
authors.affiliation: Departamento de Química, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.
authors.forename: Thanany B
authors.initials: TB
authors.lastname: da Silva
authors.affiliation: Departamento de Biologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil.
authors.forename: Ana Paula Nascimento
authors.initials: AP
authors.lastname: Prata
authors.affiliation: Departamento de Morfologia, Universidade Federal de Sergipe, CEP 49.000-100 São Cristóvão, SE, Brazil.
authors.forename: Waldecy
authors.initials: W
authors.lastname: de Lucca
authors.affiliation: Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco, 56.304-205 Petrolina, PE, Brazil.
authors.forename: Jackson R G S
authors.initials: JR
authors.lastname: Almeida
authors.affiliation: Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco, 56.304-205 Petrolina, PE, Brazil.
authors.forename: Julianeli T
authors.initials: JT
authors.lastname: Lima
authors.affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, 49.000-100 São Cristóvão, SE, Brazil ; Laboratório de Farmacologia Pré-Clinica (LAPEC), Departamento de Fisiologia, Universidade Federal de Sergipe, Avenida Tancredo Neves, S/N, Bairro, Rosa Elza, 49.000-100 São Cristóvão, SE, Brazil.
authors.forename: Jullyana S S
authors.initials: JS
authors.lastname: Quintans
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004487:Edema / Q000188:drug therapy; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D051379:Mice; D059225:Nociception / Q000187:drug effects; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D010538:Peritonitis / Q000188:drug therapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D012717:Sesquiterpenes / Q000032:analysis; D054336:Xylopia / Q000737:chemistry*
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D009822:Oils, Volatile; D010936:Plant Extracts; D016760:Proto-Oncogene Proteins c-fos; D012717:Sesquiterpenes
references.citation: Bioresour Technol. 2004 Nov;95(2):229-33
references.pmid: 15246449
references.citation: J Pain. 2007 Feb;8(2):127-36
references.pmid: 16949880
references.citation: Food Chem Toxicol. 2010 Dec;48(12):3412-7
references.pmid: 20849907
references.citation: Comb Chem High Throughput Screen. 2006 Jul;9(6):425-42
references.pmid: 16842224
references.citation: Biomed Pharmacother. 2004 Jul-Aug;58(6-7):388-92
references.pmid: 15271421
references.citation: Basic Clin Pharmacol Toxicol. 2014 Feb;114(2):188-96
references.pmid: 24119304
references.citation: Eur J Pharmacol. 1980 Jan 11;61(1):17-24
references.pmid: 7353582
references.citation: Pain. 1989 Sep;38(3):347-352
references.pmid: 2478947
references.citation: Planta Med. 2013 Jan;79(2):123-30
references.pmid: 23307235
references.citation: Nat Immunol. 2005 Dec;6(12):1182-90
references.pmid: 16369557
references.citation: Trends Neurosci. 2009 Jun;32(6):339-46
references.pmid: 19414201
references.citation: Br J Pharmacol. 1987 Aug;91(4):823-32
references.pmid: 2822190
references.citation: Methods Enzymol. 1990;186:407-21
references.pmid: 2233308
references.citation: Toxicol In Vitro. 2011 Mar;25(2):462-8
references.pmid: 21111802
references.citation: Basic Clin Pharmacol Toxicol. 2010 Dec;107(6):949-57
references.pmid: 20849525
references.citation: J Med Food. 2011 Oct;14(10):1159-66
references.pmid: 21548805
references.citation: Pharmacol Rev. 2001 Dec;53(4):597-652
references.pmid: 11734620
references.citation: Science. 1969 Apr 25;164(3878):444-5
references.pmid: 4887743
references.citation: Phytother Res. 2006 Sep;20(9):764-72
references.pmid: 16807883
references.citation: Molecules. 2011 Nov 23;16(11):9714-20
references.pmid: 22113579
references.citation: Basic Clin Pharmacol Toxicol. 2013 Apr;112(4):215-21
references.pmid: 23035741
references.citation: Chem Senses. 2001 Jun;26(5):551-76
references.pmid: 11418502
references.citation: Behav Brain Res. 2000 Nov;115(2):117-41
references.pmid: 11000416
references.citation: Life Sci. 2004 Mar 12;74(17):2157-84
references.pmid: 14969719
references.citation: Arch Immunol Ther Exp (Warsz). 2007 Sep-Oct;55(5):315-27
references.pmid: 18219762
references.citation: J Chromatogr. 1963 Aug;11:463-71
references.pmid: 14062605
references.citation: Nat Prod Commun. 2011 Jun;6(6):907-12
references.pmid: 21815437
references.citation: J Ethnopharmacol. 2008 Feb 28;116(1):125-30
references.pmid: 18164567
references.citation: Pain. 1983 Jun;16(2):109-110
references.pmid: 6877845
references.citation: Proc Soc Exp Biol Med. 1962 Dec;111:544-7
references.pmid: 14001233
references.citation: J Neurochem. 2006 Jun;97(6):1634-58
references.pmid: 16805774
references.citation: Z Naturforsch C J Biosci. 2009 Sep-Oct;64(9-10):650-6
references.pmid: 19957432
references.citation: Neurosci Biobehav Rev. 2004 Jan;27(8):729-37
references.pmid: 15019423
references.citation: Phytother Res. 2013 Jan;27(1):1-15
references.pmid: 23296806
references.citation: J Ethnopharmacol. 1999 Jul;66(1):113-7
references.pmid: 10432217
references.citation: Chem Biodivers. 2008 Feb;5(2):251-8
references.pmid: 18293438
references.citation: Pharm Biol. 2011 Jun;49(6):583-6
references.pmid: 21385090
references.citation: J Med Food. 2011 Nov;14(11):1389-96
references.pmid: 21663477
references.citation: Methods Enzymol. 1996;268:142-51
references.pmid: 8782580
references.citation: Pain. 1987 Jul;30(1):103-114
references.pmid: 3614974
references.citation: J Nat Med. 2010 Jan;64(1):9-15
references.pmid: 19730974
references.citation: Br J Pharmacol. 1988 Oct;95(2):413-8
references.pmid: 2465805
references.citation: Basic Clin Pharmacol Toxicol. 2014 Jun;114(6):442-50
references.pmid: 24252102
references.citation: Pharm Biol. 2010 Oct;48(10):1164-9
references.pmid: 20738158
references.citation: Eur J Pharmacol. 2007 Dec 8;576(1-3):171-9
references.pmid: 17719028
references.citation: Biochem Soc Trans. 2006 Nov;34(Pt 5):965-70
references.pmid: 17052238