title: Physiological features of Halomonas lionensis sp. nov., a novel bacterium isolated from a Mediterranean Sea sediment.
abstract: A novel halophilic bacterium, strain RHS90(T), was isolated from marine sediments from the Gulf of Lions, in the Mediterranean Sea. Its metabolic and physiological characteristics were examined under various cultural conditions, including exposure to stressful ones (oligotrophy, high pressure and high concentrations of metals). Based on phylogenetic analysis of the 16S rRNA gene, the strain was found to belong to the genus Halomonas in the class Gammaproteobacteria. Its closest relatives are Halomonas axialensis and Halomonas meridiana (98% similarity). DNA-DNA hybridizations indicated that the novel isolate is genotypically distinct from these species. The DNA G + C content of the strain is 54.4 mol%. The main fatty acids (C18:1ω7c, 2-OH iso-C15:0, C16:0 and/or C19:0 cyclo ω8c), main polar lipids (diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unidentified phosphoglycolipid) and major respiratory quinone (ubiquinone Q9) were determined. The novel isolate is heterotrophic, mesophilic, euryhaline (growth optimum ranging from 2 to 8% w/v NaCl) and is able to grow under stressful conditions. The strain accumulates poly-β-hydroxyalkanoates granules and compatible solutes. Based on genotypic, chemotaxonomic and phenotypic distinctiveness, this isolate is likely to represent a novel species, for which the name Halomonas lionensis is proposed. The type strain of H. lionensis is RHS90(T) (DSM 25632(T) = CIP 110370(T) = UBOCC 3186(T)).
authors.affiliation: Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM) - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; CNRS, IUEM - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; Ifremer, UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Technopôle Pointe du diable, F-29280 Plouzané, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Gaboyer
authors.affiliation: CNRS, IUEM - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM) - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; Ifremer, UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Technopôle Pointe du diable, F-29280 Plouzané, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Vandenabeele-Trambouze
authors.affiliation: Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM) - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; CNRS, IUEM - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; Ifremer, UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Technopôle Pointe du diable, F-29280 Plouzané, France.
authors.forename: Junwei
authors.initials: J
authors.lastname: Cao
authors.affiliation: Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM) - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; CNRS, IUEM - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; Ifremer, UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Technopôle Pointe du diable, F-29280 Plouzané, France.
authors.forename: Maria-Cristina
authors.initials: MC
authors.lastname: Ciobanu
authors.affiliation: Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM) - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; CNRS, IUEM - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; Ifremer, UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Technopôle Pointe du diable, F-29280 Plouzané, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jebbar
authors.affiliation: Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM) - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; CNRS, IUEM - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; Ifremer, UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Technopôle Pointe du diable, F-29280 Plouzané, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Le Romancer
authors.affiliation: CNRS, IUEM - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM) - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), rue Dumont d'Urville, F-29280 Plouzané, France; Ifremer, UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Technopôle Pointe du diable, F-29280 Plouzané, France. Electronic address: Karine.Alain@univ-brest.fr.
authors.forename: Karine
authors.initials: K
authors.lastname: Alain
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008522:Mediterranean Sea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D054813:Polyhydroxyalkanoates / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D054813:Polyhydroxyalkanoates; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
keywords: Compatible; Environmental adaptation; Halomonas; Metal tolerance; Poly-β-hydroxyalkanoate; Taxonomy