title: Extraction optimization of polysaccharide from Zanthoxylum bungeanum using RSM and its antioxidant activity.
abstract: Polysaccharide prepared from pericarp of Zanthoxylum bungeanum was proved to possess excellent antioxidant activities in vitro by using reducing ferric iron power, DPPH radical scavenging activity, chelating ferrous iron capacity, and hydroxyl radical scavenging activity assays in the present study. In those four antioxidant assay models, Z. bungeanum polysaccharide (ZBP) displayed prominent antioxidant activities with low EC50 values of 0.011, 0.021, 0.056 and 0.008 mg/mL, respectively. Moreover, the extraction process of ZBP was further optimized by response surface methodology combined with Box-Behnken design. The highest polysaccharide yield 13.96%, which agreed closely with the predicted yield 13.20%, was obtained under the optimal extraction conditions as follows: extraction temperature 89 °C, extraction time 3h, ratio of water volume (mL) to raw material weight (g) 29 (v/w), and extraction number two times. The present research not only provide theoretical basis for exploitation of natural polysaccharide antioxidants, but also establish the foundation of large-scale production and further system utilization of ZBP.
authors.affiliation: Department of Forestry Pathology, College of Forestry, Northwest Agricultural & Forestry University, No. 3, Taicheng Road, Yangling 712100, Shanxi, China. Electronic address: lipq110@163.com.
authors.forename: Peiqin
authors.initials: P
authors.lastname: Li
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Ligang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Mou
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Mao
mesh_terms: D000704:Analysis of Variance; D000975:Antioxidants / Q000494:pharmacology*; D001709:Biotechnology / Q000379:methods*; D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000494:pharmacology; D008962:Models, Theoretical; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010851:Picrates; D011134:Polysaccharides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron
keywords: Antioxidant activity; Extraction optimization; Zanthoxylum bungeanum polysaccharide