title: The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain.
abstract: The first structure of the catalytic domain of RpfC (Rv1884), one of the resuscitation-promoting factors (RPFs) from Mycobacterium tuberculosis, is reported. The structure was solved using molecular replacement once the space group had been correctly identified as twinned P21 rather than the apparent C2221 by searching for anomalous scattering sites in P1. The structure displays a very high degree of structural conservation with the previously published structures of the catalytic domains of RpfB (Rv1009) and RpfE (Rv2450). This structural conservation highlights the importance of the versatile domain composition of the RPF family.
authors.affiliation: Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences, Birkbeck, University of London, Malet Street, London WC1E 7HX, England.
authors.forename: Francois Xavier
authors.initials: FX
authors.lastname: Chauviac
authors.affiliation: Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences, Birkbeck, University of London, Malet Street, London WC1E 7HX, England.
authors.forename: Giles
authors.initials: G
authors.lastname: Robertson
authors.affiliation: Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences, Birkbeck, University of London, Malet Street, London WC1E 7HX, England.
authors.forename: Doris H X
authors.initials: DH
authors.lastname: Quay
authors.affiliation: Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences, Birkbeck, University of London, Malet Street, London WC1E 7HX, England.
authors.forename: Claire
authors.initials: C
authors.lastname: Bagnéris
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR 5048, 29 Rue de Navacelles, 34090 Montpellier, France; INSERM U1054, Université Montpellier I, Montpellier, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Dumas
authors.affiliation: Department of Microbial Diseases, UCL-Eastman Dental Institute, University College London, 256 Gray's Inn Road, London WC1X 8LD, England.
authors.forename: Brian
authors.initials: B
authors.lastname: Henderson
authors.affiliation: The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, England.
authors.forename: John
authors.initials: J
authors.lastname: Ward
authors.affiliation: Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences, Birkbeck, University of London, Malet Street, London WC1E 7HX, England.
authors.forename: Nicholas H
authors.initials: NH
authors.lastname: Keep
authors.affiliation: Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences, Birkbeck, University of London, Malet Street, London WC1E 7HX, England.
authors.forename: Martin
authors.initials: M
authors.lastname: Cohen-Gonsaud
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D020134:Catalytic Domain; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins
keywords: Mycobacterium tuberculosis; peptidoglycan; resuscitation-promoting factor
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