title: Molecular basis for the differential interaction of plant mitochondrial VDAC proteins with tRNAs.
abstract: In plants, the voltage-dependent anion-selective channel (VDAC) is a major component of a pathway involved in transfer RNA (tRNA) translocation through the mitochondrial outer membrane. However, the way in which VDAC proteins interact with tRNAs is still unknown. Potato mitochondria contain two major mitochondrial VDAC proteins, VDAC34 and VDAC36. These two proteins, composed of a N-terminal α-helix and of 19 β-strands forming a β-barrel structure, share 75% sequence identity. Here, using both northwestern and gel shift experiments, we report that these two proteins interact differentially with nucleic acids. VDAC34 binds more efficiently with tRNAs or other nucleic acids than VDAC36. To further identify specific features and critical amino acids required for tRNA binding, 21 VDAC34 mutants were constructed and analyzed by northwestern. This allowed us to show that the β-barrel structure of VDAC34 and the first 50 amino acids that contain the α-helix are essential for RNA binding. Altogether the work shows that during evolution, plant mitochondrial VDAC proteins have diverged so as to interact differentially with nucleic acids, and this may reflect their involvement in various specialized biological functions.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, UPR 2357 CNRS, associated with Strasbourg University, 12 rue du Général Zimmer 67084 Strasbourg cedex, France laurence.drouard@ibmp-cnrs.unistra.fr.
authors.forename: Thalia
authors.initials: T
authors.lastname: Salinas
authors.affiliation: Institut de Biologie Moléculaire des Plantes, UPR 2357 CNRS, associated with Strasbourg University, 12 rue du Général Zimmer 67084 Strasbourg cedex, France.
authors.forename: Samira
authors.initials: S
authors.lastname: El Farouk-Ameqrane
authors.affiliation: Institut de Biologie Moléculaire des Plantes, UPR 2357 CNRS, associated with Strasbourg University, 12 rue du Général Zimmer 67084 Strasbourg cedex, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Ubrig
authors.affiliation: Institut de Biologie Moléculaire et Cellulaire, UPR 9002 CNRS, associated with Strasbourg University, 15 rue René Descartes 67084 Strasbourg cedex, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Sauter
authors.affiliation: Institut de Biologie Moléculaire des Plantes, UPR 2357 CNRS, associated with Strasbourg University, 12 rue du Général Zimmer 67084 Strasbourg cedex, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Duchêne
authors.affiliation: Institut de Biologie Moléculaire des Plantes, UPR 2357 CNRS, associated with Strasbourg University, 12 rue du Général Zimmer 67084 Strasbourg cedex, France laurence.drouard@ibmp-cnrs.unistra.fr.
authors.forename: Laurence
authors.initials: L
authors.lastname: Maréchal-Drouard
mesh_terms: D018744:DNA, Plant / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D020033:Protein Isoforms / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism*; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism; D050994:Voltage-Dependent Anion Channels / Q000737:chemistry* / Q000378:metabolism
chemical_list: D018744:DNA, Plant; D024101:Mitochondrial Proteins; D010940:Plant Proteins; D020033:Protein Isoforms; D018749:RNA, Plant; D050994:Voltage-Dependent Anion Channels; D012343:RNA, Transfer
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