title: Rapid peptidomic profiling of peritoneal fluid by MALDI-TOF mass spectrometry for the identification of biomarkers of endometriosis.
abstract: Peptidomic profiling of peritoneal fluid by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS) may represent a promising, suitable, rapid method for early diagnosis and staging of endometriosis. In a case-control study, peritoneal fluid was collected from 23 patients affected by endometriosis (eight minimal/mild endometriosis and 15 moderate/severe endometriosis) and six "endometriosis free" women undergoing laparoscopy. MALDI-TOF mass spectra of the peptide fraction extracted from peritoneal fluid samples lead to identify biomarkers potentially suitable for discriminating between peritoneal fluid samples from women affected by minimal/mild endometriosis and those from women affected by moderate/severe endometriosis. Peptidomic analysis of peritoneal fluid samples may define putative peptide biomarkers suitable for staging endometriosis and improve our understanding of the pathogenesis of endometriosis.
authors.affiliation: a Institute of Food Sciences, National Research Council (CNR) Avellino Italy.
authors.forename: Rosa Anna
authors.initials: RA
authors.lastname: Siciliano
authors.affiliation: a Institute of Food Sciences, National Research Council (CNR) Avellino Italy.
authors.forename: Maria Fiorella
authors.initials: MF
authors.lastname: Mazzeo
authors.affiliation: a Institute of Food Sciences, National Research Council (CNR) Avellino Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Spada
authors.affiliation: a Institute of Food Sciences, National Research Council (CNR) Avellino Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Facchiano
authors.affiliation: a Institute of Food Sciences, National Research Council (CNR) Avellino Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: d'Acierno
authors.affiliation: b S-IN Soluzioni Informatiche Vicenza Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Stocchero
authors.affiliation: c Department of Obstetrics and Gynaecology, Second University of Naples Naples Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: De Franciscis
authors.affiliation: c Department of Obstetrics and Gynaecology, Second University of Naples Naples Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Colacurci
authors.affiliation: d Department of Experimental Medicine, Section of Hygiene, Occupational and Forensic Medicine - Second University of Naples Naples Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Sannolo
authors.affiliation: d Department of Experimental Medicine, Section of Hygiene, Occupational and Forensic Medicine - Second University of Naples Naples Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Miraglia
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D004715:Endometriosis / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D006801:Humans; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers
keywords: Biomarkers; MALDI-TOF-MS; endometriosis; laparoscopy; peptidomics