title: Comparative efficacy of cedarwood oil and xylene in hematoxylin and eosin staining procedures: An experimental study.
abstract: BACKGROUND
  Xylene is used as a clearing agent in hematoxylin and eosin (H and E) staining of tissue sections in routine histopathology based diagnosis. However, the hazards associated with exposure to xylene are of concern. Numerous solutions mainly essential oils have been evaluated in the past as clearing agents, which can possibly be substituted for xylene during the routine tissue processing.
  
  
  OBJECTIVE
  The aim of this study is to compare the efficacy of essential oil (cedarwood oil), as a possible replacement for xylene in H and E staining procedures.
  
  
  METHODS
  The study was carried out in the Department of Oral Pathology and Microbiology. Thirty paraffin blocks of the routine biopsy specimen were retrieved from the department archives. The cedarwood oil was procured from organic and essential oil dealer in the local market. Two to three paraffin sections of four micron thickness were cut from each of the 30 paraffin blocks of processed tissue specimens, were subjected to different clearing agents: Essential oil (8% cedarwood oil) or xylene and stained with H and E stain. The stained sections were scored based on nuclear and cytoplasmic details, clarity and uniformity of staining.
  
  
  RESULTS
  Significant correlation was observed between cedarwood oil and xylene in terms of the three staining quality parameters assessed.
  
  
  CONCLUSIONS
  We conclude that cedarwood oil can be an effective, eco-friendly and safe alternative to xylene as a clearing agent in the histopathological laboratory.
authors.affiliation: Department of Oral Pathology and Microbiology, Mahatma Gandhi Post Graduate Institute of Dental Sciences, Puducherry, India.
authors.forename: Sudip
authors.initials: S
authors.lastname: Indu
authors.affiliation: Department of Oral Pathology and Microbiology, Mahatma Gandhi Post Graduate Institute of Dental Sciences, Puducherry, India.
authors.forename: V
authors.initials: V
authors.lastname: Ramesh
authors.affiliation: Department of Nutrition and Dietitics, Achariya Arts and Science College, Puducherry, India.
authors.forename: Priyanka Chakravarty
authors.initials: PC
authors.lastname: Indu
authors.affiliation: Department of Oral Pathology and Microbiology, Mahatma Gandhi Post Graduate Institute of Dental Sciences, Puducherry, India.
authors.forename: Karthikshree V
authors.initials: KV
authors.lastname: Prashad
authors.affiliation: Department of Oral Pathology and Microbiology, Mahatma Gandhi Post Graduate Institute of Dental Sciences, Puducherry, India.
authors.forename: B
authors.initials: B
authors.lastname: Premalatha
authors.affiliation: Department of Oral Pathology and Microbiology, Mahatma Gandhi Post Graduate Institute of Dental Sciences, Puducherry, India.
authors.forename: K
authors.initials: K
authors.lastname: Ramadoss
keywords: Cedarwood oil; clearing; staining; xylene
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