title: Myomaker is essential for muscle regeneration.
abstract: Regeneration of injured adult skeletal muscle involves fusion of activated satellite cells to form new myofibers. Myomaker is a muscle-specific membrane protein required for fusion of embryonic myoblasts, but its potential involvement in adult muscle regeneration has not been explored. We show that myogenic basic helix-loop-helix (bHLH) transcription factors induce myomaker expression in satellite cells during acute and chronic muscle regeneration. Moreover, genetic deletion of myomaker in adult satellite cells completely abolishes muscle regeneration, resulting in severe muscle destruction after injury. Myomaker is the only muscle-specific protein known to be absolutely essential for fusion of embryonic and adult myoblasts.
authors.affiliation: Department of Molecular Biology, Harmon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
authors.forename: Douglas P
authors.initials: DP
authors.lastname: Millay
authors.affiliation: Department of Molecular Biology, Harmon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
authors.forename: Lillian B
authors.initials: LB
authors.lastname: Sutherland
authors.affiliation: Department of Molecular Biology, Harmon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
authors.forename: Rhonda
authors.initials: R
authors.lastname: Bassel-Duby
authors.affiliation: Department of Molecular Biology, Harmon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA eric.olson@utsouthwestern.edu douglas.millay@cchmc.org.
authors.forename: Eric N
authors.initials: EN
authors.lastname: Olson
mesh_terms: D000818:Animals; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000293:injuries / Q000502:physiology*; D017570:MyoD Protein / Q000378:metabolism; D018008:Myogenin / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D012038:Regeneration / Q000235:genetics*; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D009124:Muscle Proteins; D017570:MyoD Protein; D018008:Myogenin; C000591742:Mymk protein, mouse
keywords: E-box elements; fusion; muscle regeneration; myogenesis; myomaker
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