title: Effect of bioclimatic area on the composition and bioactivity of Tunisian Rosmarinus officinalis essential oils.
abstract: The chemical composition of eight Tunisian Rosmarinus officinalis L. populations (A-H) from different bioclimatic areas has been examined by gas chromatography (GC) and GC-mass spectrometry. The essential oils are characterised by high amounts of oxygenated monoterpenes (58.2-71.7%) followed by monoterpene hydrocabons (15.1-26.7%). 1,8-Cineole, camphor, α-pinene and borneol are the main representative components. The antioxidant activity was investigated by 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing ability power assay and β-carotene bleaching test. Samples showed antiradical activity by inhibiting DPPH radical with IC50 values ranging from 375.3 to 592.8 μg mL(- 1) for samples F and A, respectively. Sample A also showed the most promising activity in β-carotene bleaching test (IC50 of 31.9 μg mL(- 1)). The essential oils were also screened for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity. Sample G showed the highest activity against AChE (IC50 of 64.7 μg mL(- 1)) while sample D (IC50 of 29.5 μg mL(- 1)) exhibited the most potent activity against BChE.
authors.affiliation: a Laboratoire des Plantes Extremophiles - Biotechnologic Center Borj-Cedria Technopark , B.P. 901, 2050 Hammam-Lif , Tunisia.
authors.forename: Mariem
authors.initials: M
authors.lastname: Ben Jemia
authors.forename: Rosa
authors.initials: R
authors.lastname: Tundis
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pugliese
authors.forename: Francesco
authors.initials: F
authors.lastname: Menichini
authors.forename: Felice
authors.initials: F
authors.lastname: Senatore
authors.forename: Maurizio
authors.initials: M
authors.lastname: Bruno
authors.forename: Mohamed Elyes
authors.initials: ME
authors.lastname: Kchouk
authors.forename: Monica Rosa
authors.initials: MR
authors.lastname: Loizzo
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000737:chemistry; D002980:Climate; D039821:Monoterpenes / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D027542:Rosmarinus / Q000737:chemistry*; D014416:Tunisia
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D039821:Monoterpenes; D009822:Oils, Volatile; D010938:Plant Oils
keywords: GC-MS analysis; Rosmarinus officinalis; antioxidant properties; cholinesterase inhibitory activity; essential oil