title: [Hypothermia combined with dexamethasone reduces ICAM1 expression and protects spermatogenesis after testicular torsion-detorsion].
abstract: OBJECTIVE
  To investigate the protective effect of hypothermia combined with dexamethasone on spermatogenesis and the expression of intercellular adhesion molecule 1 (ICAM1) after testicular torsion-detorsion.
  
  
  METHODS
  We made unilateral testicular torsion models in 100 pubertal male Sprague-Dawley rats by 720 degree torsion of the left testis and then randomly divided them into four groups of equal number to be treated with normal temperature + physiological saline (group A), hypothermia + physiological saline (group B), normal temperature + dexamethasone (group C), and hypothermia + dexamethasone (group D). After 48 hours, we collected the testes, observed pathological changes of the testicular tissue by HE staining under the light microscope, detected the apoptosis of spermatogenic cells by TUNEL, and determined the expression of ICAM1 by Western blot.
  
  
  RESULTS
  HE staining showed different degrees of testicular tissue injury in the four groups of rats, most obvious in group A, but mild in the other three. The ICAM1 protein expression was significantly higher in group A (0.68 +/-0. 03) than in B (0. 49 +/- 0. 06, P <0. 05) , C (0. 46 +/- 0. 09, P < 0.05) , and D (0.17 +/- 0.08, P <0.01). The nuclei were deep brown or brown. Lots of apoptotic spermatogenic cells were seen in the torsion testis of group A, with a significantly higher apoptosis index ( [33. 13 +/- 3.21 ]%) than in B ( [ 17. 12 +/-5.23 ]%, P < 0.05), C ([14.13 +/- 2.03]%, P <0.05), and D ([9.05 +/- 1.03]%, P <0.01).
  
  
  CONCLUSIONS
  Hypothermia combined with dexamethasone can protect the testis from injury as well as the reproductive function of the testis after testicular torsion-detorsion and reduce the expression of ICAM1.
authors.forename: Hui-Chang
authors.initials: HC
authors.lastname: Li
authors.forename: Wu-Bin
authors.initials: WB
authors.lastname: Weng
authors.forename: Chang-Ming
authors.initials: CM
authors.lastname: Liu
authors.forename: Ben-Jiang
authors.initials: BJ
authors.lastname: Qian
authors.forename: Miao-Chun
authors.initials: MC
authors.lastname: Lin
mesh_terms: D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D004195:Disease Models, Animal; D007036:Hypothermia, Induced; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013086:Spermatic Cord Torsion / Q000378:metabolism* / Q000503:physiopathology; D013091:Spermatogenesis / Q000187:drug effects
chemical_list: D018799:Intercellular Adhesion Molecule-1; D003907:Dexamethasone