title: Identification of a unique Fe-S cluster binding site in a glycyl-radical type microcompartment shell protein.
abstract: Recently, progress has been made toward understanding the functional diversity of bacterial microcompartment (MCP) systems, which serve as protein-based metabolic organelles in diverse microbes. New types of MCPs have been identified, including the glycyl-radical propanediol (Grp) MCP. Within these elaborate protein complexes, BMC-domain shell proteins [bacterial microcompartment (in reference to the shell protein domain)] assemble to form a polyhedral barrier that encapsulates the enzymatic contents of the MCP. Interestingly, the Grp MCP contains a number of shell proteins with unusual sequence features. GrpU is one such shell protein whose amino acid sequence is particularly divergent from other members of the BMC-domain superfamily of proteins that effectively defines all MCPs. Expression, purification, and subsequent characterization of the protein showed, unexpectedly, that it binds an iron-sulfur cluster. We determined X-ray crystal structures of two GrpU orthologs, providing the first structural insight into the homohexameric BMC-domain shell proteins of the Grp system. The X-ray structures of GrpU, both obtained in the apo form, combined with spectroscopic analyses and computational modeling, show that the metal cluster resides in the central pore of the BMC shell protein at a position of broken 6-fold symmetry. The result is a structurally polymorphic iron-sulfur cluster binding site that appears to be unique among metalloproteins studied to date.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Thompson
authors.affiliation: Molecular Biology Interdepartmental Ph.D. Program, University of California, Los Angeles, CA 90095, USA.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Wheatley
authors.affiliation: University of California, Los Angeles-Department of Energy Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA.
authors.forename: Julien
authors.initials: J
authors.lastname: Jorda
authors.affiliation: University of California, Los Angeles-Department of Energy Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Sawaya
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA.
authors.forename: Soheil D
authors.initials: SD
authors.lastname: Gidaniyan
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA.
authors.forename: Hoda
authors.initials: H
authors.lastname: Ahmed
authors.affiliation: Jules Stein Eye Institute, University of California, Los Angeles, CA 90095, USA.
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA.
authors.forename: Krystal N
authors.initials: KN
authors.lastname: McCarty
authors.affiliation: Pasarow Mass Spectrometry Laboratory, Neuropsychiatric Institute-Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, CA 90095, USA.
authors.forename: Julian P
authors.initials: JP
authors.lastname: Whitelegge
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA; University of California, Los Angeles-Department of Energy Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA. Electronic address: yeates@mbi.ucla.edu.
authors.forename: Todd O
authors.initials: TO
authors.lastname: Yeates
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*; D001665:Binding Sites; D003013:Clostridium / Q000378:metabolism; D018360:Crystallography, X-Ray; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000648:ultrastructure; D008958:Models, Molecular; D044043:Pectobacterium / Q000378:metabolism; D011409:Propylene Glycols / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D011409:Propylene Glycols
keywords: BMC-domain; conformational polymorphism; glycyl-radical propanediol (Grp); metalloprotein; symmetry-breaking
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