title: Eradication of multidrug-resistantA. baumanniiin burn wounds by antiseptic pulsed electric field.
abstract: Emerging bacterial resistance to multiple drugs is an increasing problem in burn wound management. New non-pharmacologic interventions are needed for burn wound disinfection. Here we report on a novel physical method for disinfection: antiseptic pulsed electric field (PEF) applied externally to the infected burns. In a mice model, we show that PEF can reduce the load of multidrug resistantAcinetobacter baumanniipresent in a full thickness burn wound by more than four orders of magnitude, as detected by bioluminescence imaging. Furthermore, using a finite element numerical model, we demonstrate that PEF provides non-thermal, homogeneous, full thickness treatment for the burn wound, thus, overcoming the limitation of treatment depth for many topical antimicrobials. These modeling tools and ourin vivoresults will be extremely useful for further translation of the PEF technology to the clinical setting, as they provide the essential elements for planning of electrode design and treatment protocol.
authors.forename: Alexander
authors.initials: A
authors.lastname: Golberg
authors.forename: G Felix
authors.initials: GF
authors.lastname: Broelsch
authors.forename: Daniela
authors.initials: D
authors.lastname: Vecchio
authors.forename: Saiqa
authors.initials: S
authors.lastname: Khan
authors.forename: Michael R
authors.initials: MR
authors.lastname: Hamblin
authors.forename: William G
authors.initials: WG
authors.lastname: Austen
authors.forename: Robert L
authors.initials: RL
authors.lastname: Sheridan
authors.forename: Martin L
authors.initials: ML
authors.lastname: Yarmush
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