title: [Supercritical CO2 fluid extraction and component analysis of leaves oil from Taxus chinensis var. mairei].
abstract: OBJECTIVE
  To research the optimal extraction process of supercritical CO2 extraction and analyze the component of the oil extracted from leaves of Taxus chinensis var. mairei.
  
  
  METHODS
  Using the yield of leaves oil from Taxus chinensis var. mairei as the index, investigated the effect of the extraction pressure, extraction temperature and extraction time on the extracting-rate of leaves oil. The chemical composition of the extracted leaves oil was analyzed by derivatized GC-MS.
  
  
  RESULTS
  The optimal parameters of the supercritical CO2 extraction of the oil extracted from leaves of Taxus chinensis var. mairei were determined: CO2 compressor pump frequency was 10 Hz, the extraction pressure was 25 MPa and the temperature of extraction was 45 degrees C, the extraction time was 120 min, the isolator I pressure was 8.0 MPa and the temperature of extraction was 40 degrees C, the isolator II pressure was 5.0 MPa and the temperature of extraction was 35 degrees C. The extracted leaves oil was derivatized with boron trifluoride-methanol complex. Thirty-three kinds of fatty acids were identified by GC-MS.
  
  
  CONCLUSIONS
  The yield of leaves oils are different from Taxus chinensis var. mairei from 3 habitats. The yield of leaves oil from Donggang, Wuxi city is the highest, about 2.61%. The kinds of fatty acids with high amounts in leaves oil from Taxus chinensis var. mairei is identical in general, the kinds of fatty acids with low amounts in leaves oil from Taxus chinensis var. mairei have differences.
authors.forename: Bin
authors.initials: B
authors.lastname: Tang
authors.forename: Feng-su
authors.initials: FS
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Chen
authors.forename: Xiao
authors.initials: X
authors.lastname: Yao
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry; D002681:China; D025924:Chromatography, Supercritical Fluid; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry / Q000302:isolation & purification*; D011312:Pressure; D020946:Taxus / Q000737:chemistry* / Q000254:growth & development; D013696:Temperature
chemical_list: D005227:Fatty Acids; D010938:Plant Oils; D002245:Carbon Dioxide