title: Comparison of renal biomarkers with glomerular filtration rate in susceptibility to the detection of gentamicin-induced acute kidney injury in dogs.
abstract: Fourteen renal biomarkers were compared with measurement of glomerular filtration rate (GFR) in detecting acute kidney injury (AKI) in beagle dogs given gentamicin (40 mg/kg/day by subcutaneous injection) for 7 consecutive days. Serum and urinary biomarkers were measured before administration of gentamicin and then on days 4 and 8 after starting administration. GFR was derived by use of a simplified equation. Increased urinary cystatin C and decreased GFR occurred from day 4 and were detected before increases in blood urea nitrogen (BUN) and serum creatinine concentrations and changes in other urinary parameters. The closest correlation was between urinary cystatin C and GFR. At termination, microscopical examination revealed extensive necrosis of proximal tubular epithelium with hyaline casts in the kidney of treated dogs. These data indicate that urinary cystatin C is the most sensitive index of kidney injury and GFR reflects the kidney functional mass.
authors.affiliation: Co-operative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Sasaki
authors.affiliation: Co-operative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
authors.affiliation: Co-operative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Iwama
authors.affiliation: Co-operative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shimamura
authors.affiliation: Medicinal Safety Research Laboratories, Daiichi Sankyo Co., Ltd., 1-16-13 Kita-Kasai, Edogawa-ku, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yabe
authors.affiliation: Medicinal Safety Research Laboratories, Daiichi Sankyo Co., Ltd., 1-16-13 Kita-Kasai, Edogawa-ku, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takasuna
authors.affiliation: Co-operative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ichijo
authors.affiliation: Co-operative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Furuhama
authors.affiliation: Co-operative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan. Electronic address: satohsss@iwate-u.ac.jp.
authors.forename: H
authors.initials: H
authors.lastname: Satoh
mesh_terms: D058186:Acute Kidney Injury / Q000175:diagnosis* / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D055316:Cystatin C / Q000097:blood / Q000652:urine*; D004285:Dogs; D005839:Gentamicins / Q000633:toxicity; D005919:Glomerular Filtration Rate
chemical_list: D015415:Biomarkers; D055316:Cystatin C; D005839:Gentamicins
keywords: dog; gentamicin; glomerular filtration rate; kidney injury