title: Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction.
abstract: Many applications in biotechnology and molecular biology rely on modified nucleotides. Here, we present an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels-Alder reaction with inverse electron demand using a tetrazine. Labelling proceeds very efficiently and supersedes several known approaches.
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany. andreas.marx@uni-konstanz.de.
authors.forename: Holger
authors.initials: H
authors.lastname: Busskamp
authors.forename: Ellen
authors.initials: E
authors.lastname: Batroff
authors.forename: Andrea
authors.initials: A
authors.lastname: Niederwieser
authors.forename: Obadah S
authors.initials: OS
authors.lastname: Abdel-Rahman
authors.forename: Rainer F
authors.initials: RF
authors.lastname: Winter
authors.forename: Valentin
authors.initials: V
authors.lastname: Wittmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Marx
mesh_terms: D055162:Biocatalysis; D061565:Cycloaddition Reaction; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003839:Deoxyadenosines / Q000737:chemistry / Q000378:metabolism; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D004583:Electrons; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: D003839:Deoxyadenosines; D014753:Vinyl Compounds; C011381:5-vinyl-2'-deoxyuridine; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D003857:Deoxyuridine