title: Long-lived coherences: improved dispersion in the frequency domain using continuous-wave and reduced-power windowed sustaining irradiation.
abstract: Long-lived coherences (LLC's) are detectable magnetisation modes with favourable relaxation times that translate as sharp resonances upon Fourier transform. The frequency domain of LLC's was previously limited to the range of J-couplings within pairs of homonuclear spins. LLC evolution at high magnetic fields needs to be sustained by radio-frequency irradiation. We show that LLC-based spectral dispersion can be extended beyond the J-couplings domain using adapted carrier offsets and introduce a new reduced-power sustaining method to preserve LLC's within the required range of offsets. Spectral resolution is enhanced as the natively narrow lines of LLC's are further dispersed, making them potential probes for the study of biomolecules featuring strong resonance overlap and for media where NMR spectroscopy is commonly hindered by line broadening.
authors.affiliation: Laboratoire de Chimie et Biochimie Toxicologiques et Pharmacologiques UMR-8601, Université Paris Descartes - CNRS, PRES Paris Sorbonne Cité, 75006 Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Sadet
authors.affiliation: Laboratoire de Chimie et Biochimie Toxicologiques et Pharmacologiques UMR-8601, Université Paris Descartes - CNRS, PRES Paris Sorbonne Cité, 75006 Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Fernandes
authors.affiliation: Laboratoire de Chimie et Biochimie Toxicologiques et Pharmacologiques UMR-8601, Université Paris Descartes - CNRS, PRES Paris Sorbonne Cité, 75006 Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Kateb
authors.affiliation: Laboratoire de Chimie et Biochimie Toxicologiques et Pharmacologiques UMR-8601, Université Paris Descartes - CNRS, PRES Paris Sorbonne Cité, 75006 Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Balzan
authors.affiliation: Laboratoire de Chimie et Biochimie Toxicologiques et Pharmacologiques UMR-8601, Université Paris Descartes - CNRS, PRES Paris Sorbonne Cité, 75006 Paris, France.
authors.forename: P R
authors.initials: PR
authors.lastname: Vasos
mesh_terms: D000465:Algorithms; D004151:Dipeptides / Q000737:chemistry* / Q000528:radiation effects*; D017785:Photons; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D025801:Ubiquitin / Q000737:chemistry* / Q000528:radiation effects*
chemical_list: D004151:Dipeptides; D025801:Ubiquitin; C028872:alanylglycine