title: Characterization of ovalbumin absorption pathways in the rat intestine, including the effects of aspirin.
abstract: Ingested proteins are absorbed from the intestinal lumen via the paracellular and/or transcellular pathways, depending on their physicochemical properties. In this study, we investigated the absorption pathway(s) of ovalbumin (OVA), an egg white-allergen, as well as the mechanisms of aspirin-facilitated OVA absorption in rats. In situ intestinal re-circulating perfusion experiments showed that the absorption rate of fluorescein isothiocyanate (FITC)-labeled OVA in the distal intestine was higher than that for a marker of non-specific absorption, FITC-dextran (FD-40), and that colchicine, a general endocytosis inhibitor, suppressed OVA absorption. In the distal intestine, bafiromycin A1 and phenylarsine oxide inhibited the OVA absorption rate, whereas mehyl-β-cyclodextrin exerted no significant effects. Thus, OVA is preferentially absorbed from the distal intestine via the paracellular and receptor- and clathrin-mediated endocytic pathways. Furthermore, aspirin increased OVA absorption in the presence or absence of colchicine, indicating that aspirin facilitated OVA absorption by inducing intestinal barrier disruption and paracellular permeability.
authors.affiliation: Department of Pathophysiology and Therapeutics, Institute of Biomedical and Health Sciences, Hiroshima University.
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Yokooji
authors.forename: Hitomi
authors.initials: H
authors.lastname: Nouma
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Matsuo
mesh_terms: D000485:Allergens / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D001152:Arsenicals / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology*; D003078:Colchicine / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000494:pharmacology; D007408:Intestinal Absorption / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D018942:Macrolides / Q000494:pharmacology; D008297:Male; D010047:Ovalbumin / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D000485:Allergens; D001152:Arsenicals; D003911:Dextrans; D018942:Macrolides; D047392:beta-Cyclodextrins; C015219:fluorescein isothiocyanate dextran; C042851:fluorescein-ovalbumin; C108732:methyl-beta-cyclodextrin; C029341:oxophenylarsine; D012710:Serum Albumin, Bovine; C040929:bafilomycin A1; D010047:Ovalbumin; D016650:Fluorescein-5-isothiocyanate; D001241:Aspirin; D003078:Colchicine