title: Tibolone protects T98G cells from glucose deprivation.
abstract: The steroidal drug Tibolone is used for the treatment of climacteric symptoms and osteoporosis in post-menopausal women. Although Tibolone has been shown to exert neuroprotective actions after middle cerebral artery occlusion, its specific actions on glial cells have received very little attention. In the present study we have assessed whether Tibolone exerts protective actions in a human astrocyte cell model, the T98G cells, subjected to glucose deprivation. Our findings indicate that Tibolone decreases the effects of glucose deprivation on cell death, nuclear fragmentation, superoxide ion production, mitochondrial membrane potential, cytoplasmic calcium concentration and morphological parameters. These findings suggest that glial cells may participate in the neuroprotective actions of Tibolone in the brain.
authors.affiliation: Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia.
authors.forename: Marco
authors.initials: M
authors.lastname: Ávila Rodriguez
authors.affiliation: Instituto Cajal, CSIC, Avenida Doctor Arce 37, 28002 Madrid, Spain.
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: Garcia-Segura
authors.affiliation: Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Cabezas
authors.affiliation: Department of Physics and Astronomy, The University of Texas at San Antonio, United States.
authors.forename: Daniel
authors.initials: D
authors.lastname: Torrente
authors.affiliation: Laboratorio de Citoarquitectura y Plasticidad Neuronal, Instituto de Investigaciones cardiológicas Prof. Dr. Alberto C. Taquini (ININCA), Facultad de Medicina, UBA-CONICET, Marcelo T. de Alvear 2270, C1122AAJ Buenos Aires, Argentina.
authors.forename: Francisco
authors.initials: F
authors.lastname: Capani
authors.affiliation: Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia.
authors.forename: Janneth
authors.initials: J
authors.lastname: Gonzalez
authors.affiliation: Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia. Electronic address: gsampaio@javeriana.edu.co.
authors.forename: George E
authors.initials: GE
authors.lastname: Barreto
mesh_terms: D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D020847:Estrogen Receptor Modulators / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009652:Norpregnenes / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D020847:Estrogen Receptor Modulators; D018696:Neuroprotective Agents; D009652:Norpregnenes; D017382:Reactive Oxygen Species; C027385:tibolone; D005947:Glucose; D002118:Calcium
keywords: Estrogenic activity; Free radicals; Metabolic insult; Neuroprotection