title: [Induction on callus culture and regeneration of Orostachyis fimbriatae].
abstract: OBJECTIVE
  To explore the effects of different hormonal combinations on induction, proliferation and differentiation of Orostachyis fimbriatae callus culture.
  
  
  METHODS
  Aseptic seedling leaves were used as explants,the different concentrations of IAA,NAA, 6-BA and KT on induction proliferation of callus were optimized by orthogonal test to explore the optimum medium for differentiation of callus by tissue culture techniques.
  
  
  RESULTS
  The best medium for induction was MS + IAA 1.0 mg/L + NAA 0.5 mg/L + KT 1.0 mg/L, and the best hormonal combination for proliferation was MS + IAA 0.5 mg/L + 6-BA 0.5 mg/I. + KT 1.0 mg/L. The best medium for differentiation was MS + IAA 0.1 mg/L + KT 2.0 mg/L, and 1/2MS + IAA 0.2 mg/L was the optimum medium for rooting culture.
  
  
  CONCLUSIONS
  The system of regeneration of Orostachyis fimbriatae is establishd by tissue culture techniques in this study.
authors.forename: Rui-jun
authors.initials: RJ
authors.lastname: Su
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Zou
authors.forename: Qing-gui
authors.initials: QG
authors.lastname: Wu
authors.forename: Ming-hua
authors.initials: MH
authors.lastname: Luo
mesh_terms: D029627:Crassulaceae / Q000187:drug effects / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D012038:Regeneration / Q000187:drug effects*; D012639:Seeds / Q000187:drug effects / Q000502:physiology; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators