title: Investigating femtosecond-laser-induced two-photon photoacoustic generation.
abstract: We investigated two-photon absorption-based photoacoustic generation and compared it with corresponding photoluminescence emission. Experimental results revealed expected quadratic dependences on the incident optical fluence in both photoacoustic and photoluminescence processes. We also investigated the influence of optical scattering on the generation of two-photon photoacoustic and photoluminescence signals and found that photoacoustic signals attenuated more slowly than photoluminescence signals when the optical scattering coefficient was increased, which was attributed to a weaker ultrasonic attenuation than that the optical attenuation in the scattering medium. Finally, we showed three-dimensional two-photon photoacoustic imaging.
authors.affiliation: Northwestern University, Department of Biomedical Engineering, Evanston, Illinois 60208, United States.
authors.forename: Ben E
authors.initials: BE
authors.lastname: Urban
authors.affiliation: Northwestern University, Department of Biomedical Engineering, Evanston, Illinois 60208, United States.
authors.forename: Ji
authors.initials: J
authors.lastname: Yi
authors.affiliation: Texas A&M University, Department of Biomedical Engineering, College Station, Texas 77843, United States.
authors.forename: Vladislav
authors.initials: V
authors.lastname: Yakovlev
authors.affiliation: Northwestern University, Department of Biomedical Engineering, Evanston, Illinois 60208, United States.
authors.forename: Hao F
authors.initials: HF
authors.lastname: Zhang
mesh_terms: D054459:Elasticity Imaging Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D007834:Lasers; D008163:Luminescent Measurements / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D019047:Phantoms, Imaging; D061088:Photoacoustic Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
references.citation: Biophys J. 2013 Aug 20;105(4):841-7
references.pmid: 23972836
references.citation: J Biomed Opt. 2011 Oct;16(10):106014
references.pmid: 22029361
references.citation: Science. 2012 Mar 23;335(6075):1458-62
references.pmid: 22442475
references.citation: Phys Med Biol. 2009 Oct 7;54(19):R59-97
references.pmid: 19724102
references.citation: Opt Express. 2013 Sep 23;21(19):22410-22
references.pmid: 24104130
references.citation: J Biomed Opt. 2012 Jun;17(6):061202
references.pmid: 22734732
references.citation: Appl Opt. 2000 Mar 1;39(7):1194-201
references.pmid: 18338003
references.citation: Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20335-9
references.pmid: 21059930
references.citation: Opt Express. 2011 Jul 4;19(14):13365-77
references.pmid: 21747492
references.citation: Appl Spectrosc. 2004 Nov;58(11):1325-33
references.pmid: 15606938
references.citation: Biophys J. 2000 Mar;78(3):1589-98
references.pmid: 10692343
references.citation: Science. 2003 May 30;300(5624):1434-6
references.pmid: 12775841
references.citation: Med Phys. 2010 Apr;37(4):1518-21
references.pmid: 20443472
references.citation: Opt Lett. 1998 Aug 1;23(15):1238-40
references.pmid: 18087486
references.citation: Lasers Surg Med. 1992;12(5):510-9
references.pmid: 1406004
references.citation: Photochem Photobiol. 2002 Apr;75(4):327-34
references.pmid: 12003120
references.citation: Q Rev Biophys. 2005 May;38(2):97-166
references.pmid: 16478566
references.citation: Nano Lett. 2007 Apr;7(4):941-5
references.pmid: 17335272