title: Fusobacterium detected in colonic biopsy and clinicopathological features of ulcerative colitis in Japan.
abstract: OBJECTIVE
  Fusobacterium species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. Here, we performed prevalence screening of Fusobacterium in ulcerative colitis (UC) in Japanese patients.
  
  
  METHODS
  We examined Fusobacterium nucleatum (F. nucleatum) and whole Fusobacterium species (Pan-fusobacterium) by quantitative real-time PCR in 163 inflamed mucosae from 152 UC patients. Data were correlated with clinical subtypes of UC.
  
  
  RESULTS
  In an initial prevalence screen, F. nucleatum and Pan-fusobacterium were detected in 6.3 % (4/64) and 53.1 % (34/64). For all 163 mucosae, the prevalence of Pan-fusobacterium was 54.6 % (89/163). Pan-fusobacterium status was concordant in inflamed and normal adjacent samples, and the matched cases during 1-year follow-up colonoscopy. The higher amount of Pan-fusobacterium was observed in chronic continuous type compared to one attack and relapse/remitting type (p = 0.039). The higher amount of Pan-fusobacterium was also associated with rather mild clinical course of disease, such as non-steroid dependency (p = 0.015), non-refractory phenotype (p = 0.013), and non-severe phenotype (p = 0.04). Based on the distribution of Pan-fusobacterium measurable cases, we identified 10 cases as having a high amount of Pan-fusobacterium (FB-high). The clinicopathological features of FB-high UC cases were also highlighted by chronic continuous type and mild phenotypes of disease.
  
  
  CONCLUSIONS
  Whole Fusobacterium species, but not F. nucleatum, are common in UC patients and have a role in persistence of colonic inflammation in UC. However, Fusobacterium infection is associated with rather mild clinical phenotypes of UC.
authors.affiliation: Department of Gastroenterology, Fujita Health University School of Medicine, 1-98 Dengakugakubo Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan, tomomiccyu@yahoo.co.jp.
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Tahara
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Kawamura
authors.forename: Masaaki
authors.initials: M
authors.lastname: Okubo
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sumi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyata
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Nagasaka
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Naoki
authors.initials: N
authors.lastname: Ohmiya
authors.forename: Tomiyasu
authors.initials: T
authors.lastname: Arisawa
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hirata
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D003093:Colitis, Ulcerative / Q000382:microbiology* / Q000473:pathology*; D003106:Colon / Q000382:microbiology* / Q000473:pathology; D005260:Female; D005673:Fusobacterium / Q000302:isolation & purification*; D005674:Fusobacterium Infections / Q000150:complications*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D007564:Japan; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D055815:Young Adult
references.citation: J Clin Immunol. 2011 Feb;31(1):69-73
references.pmid: 20848167
references.citation: Genome Res. 2012 Feb;22(2):299-306
references.pmid: 22009989
references.citation: FEMS Immunol Med Microbiol. 2009 Jun;56(1):67-72
references.pmid: 19484811
references.citation: BMC Microbiol. 2009 Aug 20;9:171
references.pmid: 19695087
references.citation: Cytokine. 2010 Aug;51(2):173-7
references.pmid: 20621719
references.citation: Gut. 2011 Jan;60(1):34-40
references.pmid: 19926616
references.citation: Periodontol 2000. 1994 Jun;5:66-77
references.pmid: 9673163
references.citation: Altern Med Rev. 2003 Aug;8(3):247-83
references.pmid: 12946238
references.citation: FEMS Immunol Med Microbiol. 2005 Aug 1;45(2):191-9
references.pmid: 15919188
references.citation: Hum Mol Genet. 2006 Mar 1;15(5):743-9
references.pmid: 16434481
references.citation: N Engl J Med. 1990 Nov 1;323(18):1228-33
references.pmid: 2215606
references.citation: Gastroenterology. 1998 Dec;115(6):1405-13
references.pmid: 9834268
references.citation: Gut. 2001 Apr;48(4):526-35
references.pmid: 11247898
references.citation: Curr Issues Mol Biol. 2011;13(2):25-36
references.pmid: 21220789
references.citation: Clin Exp Med. 2013 Nov;13(4):239-44
references.pmid: 22955700
references.citation: Inflamm Bowel Dis. 2013 Mar;19(3):481-8
references.pmid: 23385241
references.citation: Gastroenterology. 2008 Feb;134(2):577-94
references.pmid: 18242222
references.citation: Inflamm Bowel Dis. 2011 Sep;17(9):1971-8
references.pmid: 21830275
references.citation: Gut. 2003 Jan;52(1):79-83
references.pmid: 12477765
references.citation: Genome Res. 2012 Feb;22(2):292-8
references.pmid: 22009990
references.citation: Orv Hetil. 2003 Sep 21;144(38):1853-60
references.pmid: 14596023