title: Expression of group B protective surface protein (BPS) by invasive and colonizing isolates of group B streptococci.
abstract: Group B protective surface protein (BPS) is expressed on the cell surface of some group B streptococcal (GBS) (Streptococcus agalactiae) strains and adds to the identification by capsular polysaccharide (CPS), and c or R proteins. We investigated the prevalence of BPS among GBS clinical isolates (303 invasive, 4122 colonizing) collected over 11 years in four American cities. Hot HCl cell extracts were tested by immunoprecipitation in agarose with rabbit antisera to BPS; the alpha (α) and beta (β) components of c protein; R1, R3, and R4 species of R protein; and CPS serotypes Ia-VIII. BPS was found in 155 isolates (seven invasive, 148 colonizing). Of these, 87 were Ia, 37 II, 20 V; none were III. BPS was expressed usually with another protein: a species of R by 87 or a component of c by 39. The predominant CPS/protein profiles with BPS were Ia/R1,BPS and II/c(α + β),BPS. Thus, along with CPS serotype and other surface proteins, BPS can be a valuable marker for precise strain characterization of unique GBS clinical isolates with complex surface protein profiles.
authors.affiliation: Department of Pediatrics and Laboratory Medicine and Pathology, University of Minnesota Medical School, MMC 134, 420 Delaware St. SE, Minneapolis, MN, 55455, USA.
authors.forename: Aurea E
authors.initials: AE
authors.lastname: Flores
authors.forename: G S
authors.initials: GS
authors.lastname: Chhatwal
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Hillier
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Baker
authors.forename: Patricia
authors.initials: P
authors.lastname: Ferrieri
mesh_terms: D000569:Americas; D000942:Antigens, Bacterial / Q000032:analysis* / Q000145:classification; D000954:Antigens, Surface / Q000032:analysis* / Q000145:classification; D002353:Carrier State / Q000382:microbiology; D002947:Cities; D006801:Humans; D047468:Immunoprecipitation; D016920:Meningitis, Bacterial / Q000382:microbiology; D018805:Sepsis / Q000382:microbiology; D013290:Streptococcal Infections / Q000382:microbiology*; D013292:Streptococcus agalactiae / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification*
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; C447143:group B protective surface protein, S agalactiae
references.citation: Vaccine. 2003 Jul 28;21(24):3468-72
references.pmid: 12850362
references.citation: Emerg Infect Dis. 2013 Apr;19(4):551-8
references.pmid: 23628320
references.citation: Pediatrics. 1999 Aug;104(2 Pt 1):203-9
references.pmid: 10428995
references.citation: J Clin Microbiol. 1984 Apr;19(4):506-10
references.pmid: 6201506
references.citation: J Clin Microbiol. 2002 Feb;40(2):620-6
references.pmid: 11825981
references.citation: Infect Immun. 2002 Feb;70(2):803-11
references.pmid: 11796614
references.citation: J Clin Microbiol. 2004 Mar;42(3):1326-9
references.pmid: 15004110
references.citation: APMIS. 1995 Oct;103(10):731-6
references.pmid: 8534432
references.citation: J Clin Microbiol. 2007 Sep;45(9):2909-16
references.pmid: 17634303
references.citation: Clin Microbiol Infect. 2008 Jan;14(1):66-73
references.pmid: 18034863
references.citation: Scand J Immunol. 1998 Feb;47(2):179-88
references.pmid: 9496695
references.citation: J Clin Microbiol. 1989 May;27(5):1050-4
references.pmid: 2501340
references.citation: J Clin Microbiol. 2010 Sep;48(9):3100-4
references.pmid: 20610684
references.citation: Infect Immun. 1999 May;67(5):2491-6
references.pmid: 10225912
references.citation: Appl Microbiol. 1972 Oct;24(4):669-70
references.pmid: 4628802
references.citation: J Clin Microbiol. 2004 Jan;42(1):83-9
references.pmid: 14715736
references.citation: Rev Infect Dis. 1988 Jul-Aug;10 Suppl 2:S363-6
references.pmid: 3055205
references.citation: Infect Immun. 1971 Nov;4(5):596-604
references.pmid: 5005310
references.citation: Zentralbl Bakteriol. 1996 Sep;285(1):44-51
references.pmid: 8946695
references.citation: J Clin Microbiol. 2007 Jun;45(6):1985-8
references.pmid: 17376884
references.citation: J Biol Chem. 1996 Aug 2;271(31):18892-7
references.pmid: 8702550
references.citation: Clin Infect Dis. 2000 Feb;30(2):276-81
references.pmid: 10671328
references.citation: J Exp Med. 1975 Jul 1;142(1):165-79
references.pmid: 1097573
references.citation: J Microbiol Methods. 2009 Sep;78(3):363-5
references.pmid: 19573567
references.citation: Clin Vaccine Immunol. 2013 Apr;20(4):607-12
references.pmid: 23408530
references.citation: Indian J Med Res. 2004 May;119 Suppl:191-6
references.pmid: 15232193
references.citation: Clin Vaccine Immunol. 2006 Jan;13(1):145-9
references.pmid: 16426012