title: Unique distribution of cyanobacterial podoviruses and their potential hosts in a paddy field of northeast China.
abstract: We first surveyed the DNA polymerase (pol) gene of cyanopodoviruses and the 16S-23S rRNA gene internal transcribed spacer (ITS) region of picocyanobacteria in a paddy field of northeast China. A total of 49 DNA pol clones and 76 ITS clones were obtained. The blast search results showed that all DNA pol clones and nearly 50% of the ITS clones had up to 76% and 50% identity/similarity to known sequences, respectively. Phylogenetic analyses showed that the DNA pol clones were narrowly distributed in the phylogenetic tree, and two new subclusters of cyanopodoviruses (PG-Pol-I and PG-Pol-II) specific to paddy field were discovered. In contrast, the distribution of ITS clones was very broad, and seven paddy-specific groups of picocyanobacteria (PG-Picocya-I-VII) were identified. In general, novel groups of cyanopodoviruses and picocyanobacteria were observed in this study, suggesting that coevolution between cyanopodoviruses and their hosts occurs in the paddy field.
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Wang
authors.forename: Junjie
authors.initials: J
authors.lastname: Liu
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Yu
authors.forename: Jian
authors.initials: J
authors.lastname: Jin
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Liu
mesh_terms: D002681:China; D000458:Cyanobacteria / Q000145:classification / Q000302:isolation & purification / Q000821:virology*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza; D010802:Phylogeny; D017902:Podoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012988:Soil Microbiology; D014871:Water Microbiology
chemical_list: D004259:DNA-Directed DNA Polymerase
keywords: 16S-23S rRNA gene ITS region; DNA polymerase gene; cyanopodoviruses; genetic diversity; picocyanobacteria