title: NdhO, a subunit of NADPH dehydrogenase, destabilizes medium size complex of the enzyme in Synechocystis sp. strain PCC 6803.
abstract: Two mutants that grew faster than the wild-type (WT) strain under high light conditions were isolated from Synechocystis sp. strain PCC 6803 transformed with a transposon-bearing library. Both mutants had a tag in ssl1690 encoding NdhO. Deletion of ndhO increased the activity of NADPH dehydrogenase (NDH-1)-dependent cyclic electron transport around photosystem I (NDH-CET), while overexpression decreased the activity. Although deletion and overexpression of ndhO did not have significant effects on the amount of other subunits such as NdhH, NdhI, NdhK, and NdhM in the cells, the amount of these subunits in the medium size NDH-1 (NDH-1M) complex was higher in the ndhO-deletion mutant and much lower in the overexpression strain than in the WT. NdhO strongly interacts with NdhI and NdhK but not with other subunits. NdhI interacts with NdhK and the interaction was blocked by NdhO. The blocking may destabilize the NDH-1M complex and repress the NDH-CET activity. When cells were transferred from growth light to high light, the amounts of NdhI and NdhK increased without significant change in the amount of NdhO, thus decreasing the relative amount of NdhO. This might have decreased the blocking, thereby stabilizing the NDH-1M complex and increasing the NDH-CET activity under high light conditions.
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Guilin Road 100, Shanghai 200234, China and.
authors.forename: Jiaohong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Guilin Road 100, Shanghai 200234, China and.
authors.forename: Fudan
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Guilin Road 100, Shanghai 200234, China and.
authors.forename: Jingsong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Guilin Road 100, Shanghai 200234, China and. Electronic address: wma@shnu.edu.cn.
authors.forename: Weimin
authors.initials: W
authors.lastname: Ma
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology; D004795:Enzyme Stability / Q000235:genetics; D017353:Gene Deletion; D009245:NADH Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D045331:Photosystem I Protein Complex / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D046939:Synechocystis / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D045331:Photosystem I Protein Complex; D021122:Protein Subunits; D009245:NADH Dehydrogenase
keywords: Cyanobacteria; Electron Transfer Complex; Electron Transport; Membrane Protein; NDH-1 Complexes; NDH-CET; NdhO; Photosynthesis; Stability; Synechocystis 6803
references.citation: Methods Enzymol. 1988;167:747-54
references.pmid: 3148842
references.citation: Biochim Biophys Acta. 2006 Nov;1757(11):1469-75
references.pmid: 16844076
references.citation: PLoS Biol. 2011 Apr;9(4):e1001040
references.pmid: 21483722
references.citation: J Biol Chem. 2000 Oct 13;275(41):31630-4
references.pmid: 10906128
references.citation: FEBS Lett. 2000 Aug 11;479(1-2):1-5
references.pmid: 10940377
references.citation: J Biol Chem. 2005 Jan 28;280(4):2587-95
references.pmid: 15548534
references.citation: Proc Natl Acad Sci U S A. 1991 May 15;88(10):4275-9
references.pmid: 1903537
references.citation: Science. 2006 Mar 10;311(5766):1430-6
references.pmid: 16469879
references.citation: DNA Res. 1996 Jun 30;3(3):109-36
references.pmid: 8905231
references.citation: Nature. 2010 May 27;465(7297):441-5
references.pmid: 20505720
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: Biochemistry. 2011 Feb 22;50(7):1121-4
references.pmid: 21244052
references.citation: Plant Cell. 2009 Nov;21(11):3623-40
references.pmid: 19903870
references.citation: Photosynth Res. 2007 Jul-Sep;93(1-3):69-77
references.pmid: 17279442
references.citation: Plant Physiol. 2013 Nov;163(3):1191-202
references.pmid: 24089436
references.citation: J Biol Chem. 2014 Jul 4;289(27):18770-81
references.pmid: 24847053
references.citation: Plant Cell. 2013 Oct;25(10):3926-43
references.pmid: 24096342
references.citation: Plant Physiol. 2006 Jun;141(2):465-74
references.pmid: 16428601
references.citation: Plant J. 2013 Sep;75(5):858-66
references.pmid: 23725563
references.citation: J Bacteriol. 2011 Jan;193(1):292-5
references.pmid: 21036997
references.citation: FEBS Lett. 2003 Jun 12;545(1):9-17
references.pmid: 12788486
references.citation: Plant Cell Physiol. 2003 May;44(5):534-40
references.pmid: 12773640
references.citation: Plant Cell. 2002 Nov;14(11):2659-79
references.pmid: 12417694
references.citation: Nature. 2013 Feb 28;494(7438):443-8
references.pmid: 23417064
references.citation: Plant Cell. 2005 Jan;17(1):219-32
references.pmid: 15608332
references.citation: Biochim Biophys Acta. 2011 Aug;1807(8):935-44
references.pmid: 21035426
references.citation: Plant Cell Physiol. 2011 Sep;52(9):1560-8
references.pmid: 21785130
references.citation: FEBS Lett. 1995 Jun 26;367(2):107-11
references.pmid: 7796904
references.citation: Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9705-9
references.pmid: 9689145
references.citation: Plant Cell. 2011 Apr;23(4):1480-93
references.pmid: 21505067
references.citation: J Biol Chem. 2009 Jan 9;284(2):905-12
references.pmid: 18974055
references.citation: Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):8787-91
references.pmid: 8090724
references.citation: Plant Cell. 2001 Apr;13(4):793-806
references.pmid: 11283337
references.citation: Biochim Biophys Acta. 1998 May 6;1364(2):125-33
references.pmid: 9593856
references.citation: Photosynth Res. 2001;70(2):167-73
references.pmid: 16228350
references.citation: Plant J. 2003 Nov;36(4):541-9
references.pmid: 14617084
references.citation: Biochim Biophys Acta. 2011 Aug;1807(8):945-53
references.pmid: 21029720
references.citation: Biochim Biophys Acta. 2010 Sep;1797(9):1681-6
references.pmid: 20547137
references.citation: Mol Plant. 2009 Nov;2(6):1127-40
references.pmid: 19995722
references.citation: FEBS Lett. 1999 Aug 20;457(1):5-8
references.pmid: 10486552
references.citation: Biochem J. 2005 Sep 1;390(Pt 2):513-20
references.pmid: 15910282
references.citation: J Biol Chem. 2011 Oct 21;286(42):36992-7001
references.pmid: 21880717
references.citation: EMBO J. 1998 Feb 16;17(4):868-76
references.pmid: 9463365
references.citation: Plant Cell. 2005 May;17(5):1569-84
references.pmid: 15805487
references.citation: J Phycol. 1968 Mar;4(1):1-4
references.pmid: 27067764
references.citation: Physiol Plant. 2007 Sep;131(1):22-32
references.pmid: 18251921
references.citation: Plant Cell. 2012 Jan;24(1):202-14
references.pmid: 22274627