title: Identification and detection of three new F17 fimbrial variants in Escherichia coli strains isolated from cattle.
abstract: F17 fimbriae are produced by pathogenic Escherichia coli involved in diarrhea and septicemia outbreaks in calves and lambs. These proteins result from the expression of four different clustered genes, namely f17A, f17D, f17C and f17G, encoding a pilin protein, a periplasmic protein, an anchor protein and an adhesin protein, respectively. Several variants of f17A and f17G genes have been reported and found genetically associated with typical virulence factors of bovine pathogenic E. coli strains. In this study, a new F17e-A variant, closely related to F17b-A, was identified from a collection of 58 E. coli isolates from diarrheic calves in Iran. While highly prevalent in Iranian F17-producing clinical isolates from calves, this variant was rare among E. coli from a French healthy adult bovine population, suggesting a possible association with virulence. The f17Ae gene was also found in the genome of the Shiga-like toxin variant Stx1d-producing bovine E. coli strain MHI813, and belonged to a gene cluster also encoding a new F17-G3 variant, which greatly differed from F17-G1 and F17-G2. This gene cluster was located on a pathogenicity island integrated in the tRNA pheV gene. The gene coding for a third new F17f-A variant corresponding to a combination of F17c-A and F17d-A was also identified on the pVir68 plasmid in the bovine pathogenic E. coli strain 6.0900. In conclusion, we identified three new F17-A and F17-G variants in cattle E. coli, which may also have significant impact on the development of new diagnostics and vaccination tools.
authors.forename: Morgan
authors.initials: M
authors.lastname: Bihannic
authors.forename: Reza
authors.initials: R
authors.lastname: Ghanbarpour
authors.forename: Frédéric
authors.initials: F
authors.lastname: Auvray
authors.forename: Laurent
authors.initials: L
authors.lastname: Cavalié
authors.forename: Pierre
authors.initials: P
authors.lastname: Châtre
authors.forename: Michèle
authors.initials: M
authors.lastname: Boury
authors.forename: Hubert
authors.initials: H
authors.lastname: Brugère
authors.affiliation: Unité Antibiorésistance et Virulence Bactériennes, Anses Lyon, Lyon, France. jean-yves.madec@anses.fr.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Madec
authors.forename: Eric
authors.initials: E
authors.lastname: Oswald
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics* / Q000382:microbiology; D003967:Diarrhea / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D018830:Adhesins, Escherichia coli; D029968:Escherichia coli Proteins
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