title: Determination of Water-Soluble Components of Abdominal Secretion of Grasshopper (Chorthippusspp.) by GC/MS/MS in Search for Potential Wound Healing Agents.
abstract: Wound healing is still a serious medical problem due to process complexity and lack of effective medicaments. This is particularly true in the treatment of wounds arising in the course of such diseases as AIDS or diabetes. Therefore, scientific efforts are focused on the search for new compounds of natural origin, which could be used as medicines or evaluated for subsequent drug design. In folk medicine, grasshopper (Chorthippusspp.) abdominal secretion has been used to accelerate the wound healing process. In this context, the knowledge of the composition of grasshopper abdominal secretion is crucial. The aim of this study was to determine the main water-soluble components of grasshopper abdominal secretion with the use of GC/MS/MS. Liquid-liquid extraction was used as a pretreatment method to clean up, concentrate and fractionate compounds from the complex insect matrix. To obtain more stable and volatile compounds, necessary for GC/MS/MS analysis, a double-step derivatization process was carried out with the use of methoxyamine hydrochloride and a mixture of bis-N,O-trimethylsilyl trifluoroacetamide and chlorotrimethylsilane. As a result, 2,108 compounds were identified, mainly as amino acids, carbohydrates and organic acids. Some of the identified compounds are emphasized due to antimicrobial, antifungal or antioxidant activities reported in the literature. Moreover, a set of compounds characteristic forChorthippusspp. samples has been selected. In the last part of the study, a statistical analysis was performed to demonstrate differences in composition of aqueous fractions of abdominal secretions from grasshoppers collected at two distant locations: Starogard Gdański and Łubianka meadows.
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Buszewska-Forajta
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Wiktoria
authors.initials: W
authors.lastname: Struck-Lewicka
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Bujak
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Siluk
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Kaliszan
keywords: Chorthippus spp.; GC/MS/MS; Grasshoppers’ abdominal secretion; Metabolomics; Water-soluble compounds; Wound healing
references.citation: J Nutr. 2004 Jun;134(6 Suppl):1558S-1565S
references.pmid: 15173430
references.citation: Biochim Biophys Acta. 2000 Oct 18;1482(1-2):102-9
references.pmid: 11058752
references.citation: Nat Protoc. 2011 Jun 30;6(7):1060-83
references.pmid: 21720319
references.citation: J Surg Res. 1970 Oct;10(10):459-64
references.pmid: 5476457
references.citation: Metabolomics. 2011 Sep;7(3):307-328
references.pmid: 21949491
references.citation: Nephrol Dial Transplant. 2005 Dec;20(12):2836-8
references.pmid: 16221705
references.citation: J Chin Med Assoc. 2006 May;69(5):207-12
references.pmid: 16835982
references.citation: Curr Opin Clin Nutr Metab Care. 2008 May;11(3):281-8
references.pmid: 18403925
references.citation: Appl Environ Microbiol. 1983 Jan;45(1):302-4
references.pmid: 6824318
references.citation: J Insect Sci. 2010;10:92
references.pmid: 20673116
references.citation: Lipids. 2001 Nov;36(11):1283-7
references.pmid: 11795862
references.citation: BMC Bioinformatics. 2011 May 04;12:131
references.pmid: 21542920
references.citation: Clin Chim Acta. 2009 Feb;400(1-2):63-9
references.pmid: 19010317
references.citation: J Pharm Biomed Anal. 2014 Feb;89:56-66
references.pmid: 24247089
references.citation: Biochim Biophys Acta. 2002 Jul 18;1587(2-3):133-44
references.pmid: 12084455
references.citation: J Chromatogr A. 2007 Jul 27;1158(1-2):318-28
references.pmid: 17466315