title: Quantitative fluorescence ratio imaging of intralysosomal chloride ions with single excitation/dual maximum emission.
abstract: Fluorescence ratio imaging is currently being used to quantitatively detect biologically active molecules in biosystems; however, two excitations of most existing fluorescent ratiometric probes account for cumbersome operating conditions for imaging. Thus, a fluorescent ratiometric probe, 6-methoxyquinolinium-dansyl (MQ-DS), for Cl(-) with single excitation/dual maximum emission has been developed. MQ-DS can preferably localize into lysosomes and display excellent photostability. Upon excitation at a single wavelength, it responds precisely and instantaneously to changes in Cl(-) concentrations, and it can be conveniently utilized to implement real-time fluorescence ratio imaging to quantitatively track alterations in Cl(-) levels inside cells treated under various pH conditions, and also in zebrafish with acute wounds. The successful application of the new probe in bioimaging may greatly facilitate a complete understanding of the physiological functions of Cl(-) .
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014 (P.R. China).
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Shan
authors.initials: S
authors.lastname: Zhang
authors.forename: Nannan
authors.initials: N
authors.lastname: Fan
authors.forename: Haibin
authors.initials: H
authors.lastname: Xiao
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
mesh_terms: D000818:Animals; D002712:Chlorides / Q000737:chemistry* / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008247:Lysosomes / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D002712:Chlorides
keywords: anions; chlorine; fluorescence; imaging agents; lysosomes