title: Metabolic effects of hypoxia in colorectal cancer by 13C NMR isotopomer analysis.
abstract: (13)C NMR isotopomer analysis was used to characterize intermediary metabolism in three colorectal cancer cell lines (WiDr, LS1034, and C2BBe1) and determine the "metabolic remodeling" that occurs under hypoxia. Under normoxia, the three colorectal cancer cell lines present high rates of lactate production and can be seen as "Warburg" like cancer cells independently of substrate availability, since such profile was dominant at both high and low glucose media contents. The LS1034 was the less glycolytic of the three cell lines and was the most affected by the event of hypoxia, raising abruptly glucose consumption and lactate production. The other two colorectal cell lines, WiDr and C2BBe1, adapted better to hypoxia and were able to maintain their oxidative fluxes even at the very low levels of oxygen. These differential metabolic behaviors of the three colorectal cell lines show how important an adequate knowledge of the "metabolic remodeling" that follows a given cancer treatment is towards the correct (re)design of therapeutic strategies against cancer.
authors.affiliation: Biophysics Unit, IBILI, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal ; Centre of Investigation on Environment, Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3001-301 Coimbra, Portugal.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Abrantes
authors.affiliation: Life Sciences Department, Faculty of Sciences and Technology, University of Coimbra, 3000-456 Coimbra, Portugal.
authors.forename: Ludgero C
authors.initials: LC
authors.lastname: Tavares
authors.affiliation: Biophysics Unit, IBILI, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal ; Faculty of Sciences and Technology, University of Coimbra, 3000-456 Coimbra, Portugal.
authors.forename: Salomé
authors.identifier: 0000-0001-7994-4650
authors.initials: S
authors.lastname: Pires
authors.affiliation: Biophysics Unit, IBILI, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal ; Radiotherapy Department, CHUC, 3000-075 Coimbra, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Casalta-Lopes
authors.affiliation: Laboratory of Biochemical Genetics (CNC/UC), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: Cândida
authors.initials: C
authors.lastname: Mendes
authors.affiliation: Laboratory of Biochemical Genetics (CNC/UC), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: Marta
authors.identifier: 0000-0003-3998-1611
authors.initials: M
authors.lastname: Simões
authors.affiliation: Laboratory of Biochemical Genetics (CNC/UC), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal ; Center for Neuroscience and Cell Biology (CNC), 3004-517 Coimbra, Portugal.
authors.forename: Manuela M
authors.initials: MM
authors.lastname: Grazina
authors.affiliation: Life Sciences Department, Faculty of Sciences and Technology, University of Coimbra, 3000-456 Coimbra, Portugal ; Center for Neuroscience and Cell Biology (CNC), 3004-517 Coimbra, Portugal.
authors.forename: Rui A
authors.initials: RA
authors.lastname: Carvalho
authors.affiliation: Biophysics Unit, IBILI, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal ; Centre of Investigation on Environment, Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3001-301 Coimbra, Portugal.
authors.forename: Maria Filomena
authors.initials: MF
authors.lastname: Botelho
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D005947:Glucose / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D019344:Lactic Acid / Q000096:biosynthesis / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*
chemical_list: D019344:Lactic Acid; D005947:Glucose; D010100:Oxygen
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