title: Increasing Confidence of LC-MS Identifications by Utilizing Ion Mobility Spectrometry.
abstract: Ion mobility spectrometry in conjunction with liquid chromatography separations and mass spectrometry offers a range of new possibilities for analyzing complex biological samples. To fully utilize the information obtained from these three measurement dimensions, informatics tools based on the accurate mass and time tag methodology were modified to incorporate ion mobility spectrometry drift times for peptides observed in human serum. In this work a reference human serum database was created for 12,139 peptides and populated with the monoisotopic mass, liquid chromatography normalized elution time, and ion mobility spectrometry drift time(s) for each. We demonstrate that the use of three dimensions for peak matching during the peptide identification process resulted in an increased numbers of identifications and a lower false discovery rate relative to only using the mass and normalized elution time dimensions.
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Crowell
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Erin S
authors.initials: ES
authors.lastname: Baker
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Samuel H
authors.initials: SH
authors.lastname: Payne
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Yehia M
authors.initials: YM
authors.lastname: Ibrahim
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Monroe
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Gordon W
authors.initials: GW
authors.lastname: Slysz
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Brian L
authors.initials: BL
authors.lastname: LaMarche
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Vladislav A
authors.initials: VA
authors.lastname: Petyuk
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Paul D
authors.initials: PD
authors.lastname: Piehowski
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: William F
authors.initials: WF
authors.lastname: Danielson
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Anderson
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
keywords: AMT tag; LC-IMS-MS; LC-MS; drift time; false discovery rate; ion mobility spectrometry; mass spectrometry; peptide identification
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