title: Full-scale bioreactor pretreatment of highly toxic wastewater from styrene and propylene oxide production.
abstract: The wastewater originating from simultaneous production of styrene and propylene oxide (SPO) is classified as highly polluted with chemical oxygen demand level in the range 5965 to 9137mgL(-1)-as well as highly toxic. The dilution factor providing for a 10 percent toxic effect of wastewater samples in a test with Paramecium caudatum was 8.0-9.5. Biological approach for pretreatment and detoxification of the wastewater under full-scale bioreactor conditions was investigated. The number of suspended microorganisms and the clean up efficiency were increased up to 5.5-6.58×10(8)CFUmL(-1) and 88 percent, respectively during the bioreactor's operation. Isolates in the Citrobacter, Burkholderia, Pseudomonas, and Paracoccus genera were dominant in the mature suspended, as well as the immobilized microbial community of the bioreactor. The most dominant representatives were tested for their ability to biodegrade the major components of the SPO wastewater and evidence of their role in the treatment process was demonstrated. The investigated pretreatment process allowed the wastewater to be detoxified for conventional treatment with activated sludge and was closely related to the maturation of the bioreactor's microbial community.
authors.affiliation: Department of Microbiology, Kazan (Volga Region) Federal University, 420008 Kazan, Russian Federation. Electronic address: linhdao.kpfu@gmail.com.
authors.forename: Linh
authors.initials: L
authors.lastname: Dao
authors.affiliation: Department of Microbiology, Kazan (Volga Region) Federal University, 420008 Kazan, Russian Federation.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Grigoryeva
authors.affiliation: Department of Microbiology, Kazan (Volga Region) Federal University, 420008 Kazan, Russian Federation.
authors.forename: Alexander
authors.initials: A
authors.lastname: Laikov
authors.affiliation: Department of Microbiology, Kazan (Volga Region) Federal University, 420008 Kazan, Russian Federation.
authors.forename: Ruslan
authors.initials: R
authors.lastname: Devjatijarov
authors.affiliation: Department of Microbiology, Kazan (Volga Region) Federal University, 420008 Kazan, Russian Federation.
authors.forename: Olga
authors.initials: O
authors.lastname: Ilinskaya
mesh_terms: D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D004852:Epoxy Compounds; D006256:Hazardous Waste; D059013:Microbial Consortia; D020058:Styrene; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004852:Epoxy Compounds; D006256:Hazardous Waste; D020058:Styrene; D062065:Wastewater; C009068:propylene oxide
keywords: Full-scale bioreactor; Microbial diversity; Petrochemical wastewater; Propylene oxide; Styrene; Toxicity