title: Relationships between molecular mobility, fibrillogenesis of collagen molecules, and the inflammatory response: an experimental study in vitro and in vivo.
abstract: This study was designed to investigate the in vitro adsorption and fibrillogenesis of collagen on a surface with dynamic properties and to investigate how this surface affected the inflammatory response in vivo. Investigation of collagen-surface interactions is directly related to the control of wound healing where collagen adsorption, fibrillization, deposition, and maturation occur. ABA-type block copolymers, composed of polyrotaxane (which possesses α-cyclodextrin threaded along poly(ethylene glycol)) and hydrophobic terminal segments, were used to prepare mobile surfaces with representative dynamic properties. Analyses using a quartz crystal microbalance with dissipation monitoring (QCM-D) indicated that increasing the mobility of the polymer on the surface led to the formation of a soft collagen layer. The collagens in this layer had rearranged, leading to the formation of thicker collagen fibrils by lateral aggregation. When a surface with a high molecular mobility was subcutaneously implanted into rats, collagen rearrangement occurred leading to suppression of macrophage recruitment at the interface and the formation of a fibrotic capsule around the implant. These results suggest that surface mobility on an implant is an important parameter for normal wound healing.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan; Japan Science and Technology Agency, CREST, 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan.
authors.forename: Kwangwoo
authors.initials: K
authors.lastname: Nam
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan; Japan Science and Technology Agency, CREST, 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Seo
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan; Japan Science and Technology Agency, CREST, 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kimura
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan; Japan Science and Technology Agency, CREST, 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yui
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan; Japan Science and Technology Agency, CREST, 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan. Electronic address: kishida.mbme@tmd.ac.jp.
authors.forename: Akio
authors.initials: A
authors.lastname: Kishida
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D003505:Cyclodextrins / Q000737:chemistry / Q000494:pharmacology; D020442:Poloxamer / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D043862:Rotaxanes / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D014945:Wound Healing / Q000187:drug effects*; D047391:alpha-Cyclodextrins / Q000737:chemistry / Q000494:pharmacology
chemical_list: D003505:Cyclodextrins; D043862:Rotaxanes; D047391:alpha-Cyclodextrins; C475673:polyrotaxane; D020442:Poloxamer; D011092:Polyethylene Glycols; D003094:Collagen
keywords: Collagen; Fibrillogenesis; Inflammatory response; Molecular mobility; Polyrotaxane