title: Evaluation of MODS assay for rapid detection of Mycobacterium tuberculosis resistance to second-line drugs in a tertiary care tuberculosis hospital in China.
abstract: In this study we evaluated the performance of microscopic observation drug susceptibility (MODS) assay for rapid detection of Mycobacterium tuberculosis resistance to second-line drugs. 246 multidrug-resistant M. tuberculosis clinical isolates were used to compare MODS with the agar proportion method for rapid detection of resistance to 8 second-line drugs: ofloxacin, amikacin, kanamycin, capreomycin, ethionamide, cycloserine, ciprofloxacin and para-aminosalicylic acid. The sensitivity of the MODS for different drugs ranged from 88.1% to 100%, whereas the specificity ranged from 92.3% to 100%. Results for MODS assay were obtained in a median time of 7 days (range 5-18). Thus MODS assay could be used as a fast, reliable and inexpensive method for detection of M. tuberculosis resistance to second-line drugs in resource-limited settings.
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Zikun
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Tuberculosis, Jiangxi Chest Hospital, Nanchang, Jiangxi, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Weiting
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Department of Clinical Laboratory, Jiangxi Chest Hospital, Nanchang, Jiangxi, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Xiong
authors.affiliation: Department of Respiratory Medicine, Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China. Electronic address: lisir361@163.com.
authors.forename: Junming
authors.initials: J
authors.lastname: Li
mesh_terms: D000995:Antitubercular Agents / Q000627:therapeutic use*; D002681:China; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D008853:Microscopy; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D062606:Tertiary Care Centers; D013997:Time Factors; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents
keywords: Drug resistance; MODS; Mycobacterium tuberculosis; Second-line drugs