title: Mesoflavibacter sabulilitoris sp. nov., isolated from seashore sand.
abstract: A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, gliding and rod-shaped bacterial strain, designated GJMS-9(T), was isolated from seashore sand collected at Geoje island in the South Sea, South Korea. Strain GJMS-9(T) grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJMS-9(T) clustered with the type strain of Mesoflavibacter zeaxanthinifaciens, showing the highest sequence similarity of 99.1%. Strain GJMS-9(T) exhibited 16S rRNA gene sequence similarity of 96.5% to the type strain of Mesoflavibacter aestuarii and of less than 96.1% to the type strains of other recognized species. Strain GJMS-9(T) contained MK-6 as the only menaquinone and iso-C(15:1) G, iso-C(16:0) 3-OH, iso-C(15:0) and iso-C(17:0) 3-OH as the major fatty acids. The polar lipid profile of strain GJMS-9(T) containing phosphatidylethanolamine, one unidentified lipid and one unidentified glycolipid as major components was similar to that of the type strain of M. zeaxanthinifaciens. The DNA G+C content of strain GJMS-9(T) was 32.2 mol% and its DNA-DNA relatedness with M. zeaxanthinifaciens DSM 18436(T) was 38 ± 6.1%. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJMS-9(T) is separated from other species of the genus Mesoflavibacter. On the basis of the data presented, strain GJMS-9(T) is considered to represent a novel species of the genus Mesoflavibacter, for which the name Mesoflavibacter sabulilitoris sp. nov. is proposed. The type strain is GJMS-9(T) ( =KCTC 42117(T) =CECT 8597(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Ja-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon, Republic of Korea.
authors.forename: Chi Nam
authors.initials: CN
authors.lastname: Seong
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006017:Glycolipids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6; D012822:Silicon Dioxide