title: Inoculation of starter cultures in a semi-dry coffee (Coffea arabica) fermentation process.
abstract: The aim of this study was to evaluate the use of yeasts as starter cultures in coffee semi-dry processing. Arabica coffee was inoculated with one of the following starter cultures: Saccharomyces cerevisiae UFLA YCN727, S. cerevisiae UFLA YCN724, Candida parapsilosis UFLA YCN448 and Pichia guilliermondii UFLA YCN731. The control was not inoculated with a starter culture. Denaturing gradient gel electrophoresis (DGGE) was used to assess the microbial population, and organic acids and volatile compounds were quantified by HPLC and HS-SPME/GC, respectively. Sensory analyses were evaluated using the Temporal Dominance of Sensations (TDS). DGGE analysis showed that the inoculated yeasts were present throughout the fermentation. Other yeast species were also detected, including Debaryomyces hansenii, Cystofilobasidium ferigula and Trichosporon cavernicola. The bacterial population was diverse and was composed of the following genera: Weissella, Leuconostoc, Gluconobacter, Pseudomonas, Pantoea, Erwinia and Klebsiella. Butyric and propionic acids, were not detected in any treatment A total of 47 different volatiles compounds have been identified. The coffee inoculated with yeast had a caramel flavor that was not detected in the control, as assessed by TDS. The use of starter cultures during coffee fermentation is an interesting alternative for obtaining a beverage quality with distinctive flavor.
authors.affiliation: Food Sciences Department, Federal University of Lavras, CEP 37200-000 Lavras, MG, Brazil.
authors.forename: Suzana Reis
authors.initials: SR
authors.lastname: Evangelista
authors.affiliation: Biology Department, Federal University of Lavras, CEP 37200-000 Lavras, MG, Brazil.
authors.forename: Maria Gabriela da Cruz Pedrozo
authors.initials: MG
authors.lastname: Miguel
authors.affiliation: Biology Department, Federal University of Lavras, CEP 37200-000 Lavras, MG, Brazil.
authors.forename: Cecília de Souza
authors.initials: Cde S
authors.lastname: Cordeiro
authors.affiliation: Biology Department, Federal University of Lavras, CEP 37200-000 Lavras, MG, Brazil.
authors.forename: Cristina Ferreira
authors.initials: CF
authors.lastname: Silva
authors.affiliation: Food Sciences Department, Federal University of Lavras, CEP 37200-000 Lavras, MG, Brazil.
authors.forename: Ana Carla Marques
authors.initials: AC
authors.lastname: Pinheiro
authors.affiliation: Biology Department, Federal University of Lavras, CEP 37200-000 Lavras, MG, Brazil. Electronic address: rosane.schwan@pq.cnpq.br.
authors.forename: Rosane Freitas
authors.initials: RF
authors.lastname: Schwan
mesh_terms: D000438:Alcohols / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D040503:Coffea / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D003069:Coffee / Q000737:chemistry; D005285:Fermentation; D006801:Humans; D013649:Taste; D015003:Yeasts / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000438:Alcohols; D000447:Aldehydes; D003069:Coffee
keywords: Metabolites; PCR-DGGE; Semi-dry processing; Yeast