title: Apoptotic Effects of Quercitrin on DLD-1 Colon Cancer Cell Line.
abstract: Quercetin, which is the most abundant bioflavonoid compound, is mainly present in the glycoside form of quercitrin. Although different studies indicated that quercitrin is a potent antioxidant, the action of this compound is not well understood. In this study, we investigated whether quercitrin has apoptotic and antiproliferative effects in DLD-1 colon cancer cell lines. Time and dose dependent antiproliferative and apoptotic effects of quercitrin were subsequently determined by WST-1 cell proliferation assay, lactate dehydrogenase (LDH) cytotoxicity assay, detection of nucleosome enrichment factor, changes in caspase-3 activity, loss of mitochondrial membrane potential (MMP) and also the localization of phosphatidylserine (PS) in the plasma membrane. There were significant increases in caspase-3 activity, loss of MMP, and increases in the apoptotic cell population in response to quercitrin in DLD-1 colon cancer cells in a time- and dose-dependent manner. These results revealed that quercitrin has antiproliferative and apoptotic effects on colon cancer cells. Quercitrin activity supported with in vivo analyses could be a biomarker candicate for early colorectal carcinoma.
authors.affiliation: Istanbul University, Institute of Experimental Medical Research, Department of Molecular Medicine, Capa, Istanbul, Turkey.
authors.forename: Zeynep Birsu
authors.initials: ZB
authors.lastname: Cincin
authors.forename: Miray
authors.initials: M
authors.lastname: Unlu
authors.forename: Bayram
authors.initials: B
authors.lastname: Kiran
authors.forename: Elif Sinem
authors.initials: ES
authors.lastname: Bireller
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Baran
authors.forename: Bedia
authors.initials: B
authors.lastname: Cakmakoglu
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D053148:Caspase 3 / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000502:physiology; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; C012526:quercitrin; D011794:Quercetin; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3
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