title: Local and systemic immune responses induced by a recombinant chimeric protein containing Mycoplasma hyopneumoniae antigens fused to the B subunit of Escherichia coli heat-labile enterotoxin LTB.
abstract: A multi-antigen chimera composed of three antigens of Mycoplasma hyopneumoniae (R1, P42, and NrdF) and the mucosal adjuvant Escherichia coli heat-labile enterotoxin B subunit (LTB) was constructed, and its antigenic and immunogenic properties were evaluated in mice and pigs. In addition, we compared the effect of the fusion and co-administration of these proteins in mice. Antibodies against each subunit recognized the chimeric protein. Intranasal and intramuscular immunization of mice with the chimeric protein significantly increased IgG and IgA levels in the serum and tracheobronchial lavages, respectively, against some of the antigens present in the chimeric. Swine immunized with the chimeric protein developed an immune response against all M. hyopneumoniae antigens present in the fusion with a statistically significant difference (P<0.05). The adjuvant rLTB enhanced the immune response in both fused and co-administered antigens; however, better results were obtained with the chimeric protein. This multi-antigen is a promising vaccine candidate that may help control M. hyopneumoniae infection.
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, RS, Brazil. Electronic address: silmarchioro@hotmail.com.
authors.forename: Silvana Beutinger
authors.initials: SB
authors.lastname: Marchioro
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, RS, Brazil.
authors.forename: Andressa
authors.initials: A
authors.lastname: Fisch
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, RS, Brazil.
authors.forename: Charles K
authors.initials: CK
authors.lastname: Gomes
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, RS, Brazil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Jorge
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, RS, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Galli
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Freddy
authors.initials: F
authors.lastname: Haesebrouck
authors.affiliation: Unit of Porcine Health Management, Department of Reproduction, Obstetrics and Herd Health, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Dominiek
authors.initials: D
authors.lastname: Maes
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, RS, Brazil.
authors.forename: Odir
authors.initials: O
authors.lastname: Dellagostin
authors.affiliation: Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, RS, Brazil.
authors.forename: Fabricio R
authors.initials: FR
authors.lastname: Conceição
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D004768:Enterotoxins / Q000737:chemistry / Q000276:immunology*; D004926:Escherichia coli / Q000737:chemistry; D005260:Female; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D045705:Mycoplasma hyopneumoniae / Q000737:chemistry / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D013552:Swine; D014611:Vaccination
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D004768:Enterotoxins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins
keywords: Adjuvant; Bacterial enterotoxins; Fused protein; Swine enzootic pneumonia