title: [Rapid propagation system for tissure culture of Smilax glabra].
abstract: OBJECTIVE
  Establishing the rapid propagation for plant tissue culture of Smilax glabra in order to rationally protect and utilize the resource.
  
  
  METHODS
  The seed, shoot, leaf and rhizome of Similax glabra were cultured as explants. Different media and plant growth regulators at different concerntration were used.
  
  
  RESULTS
  The best medium for bud induction was MS + 6-BA 2.0 mg/L + NAA 0.05 mg/L; The optimal medium for proliferation culture was MS +6-BA 2.0 mg/L + NAA 0.5 mg/L; The optimal medium for rooting was H + IBA 0.5 mg/L + NAA 0.5 mg/L; The optimal stroma for seedlings was 50% peat + 50% river sand.
  
  
  CONCLUSIONS
  Different explants except leaf can be induced for tissue culture and rhizome has the optimal bud induction rate. An effective approach for rapid propagation has been provided,which can promote the artificial cultivation of Smilax glabra.
authors.forename: Qing-song
authors.initials: QS
authors.lastname: Dong
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Yan
authors.forename: Long-hua
authors.initials: LH
authors.lastname: Bai
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Wu
mesh_terms: D003470:Culture Media / Q000737:chemistry; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development; D027343:Rhizome / Q000187:drug effects / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000254:growth & development; D032281:Smilax / Q000187:drug effects / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators