title: Kingella kingae sequence type-complex 14 arthritis in a 16-month-old child in Greece.
abstract: We describe the first case of Kingella kingae arthritis in a 16-month-old girl in Greece, which has been diagnosed by novel molecular techniques. A joint aspiration of her knee was performed before the initiation of antibiotics, as well as on the 5th and 14th day of empiric antimicrobial therapy. The synovial fluid white blood cell count decreased from 65,000 to 1500 cells/mm, but the percentage of neutrophils remained 90% in all 3 specimens. Molecular analysis of the synovial fluid specimens by real-time polymerase chain reaction and multilocus sequence typing enabled us to reveal the presence of K. kingae belonging to the international sequence type-complex 14, which persisted up to the fifth day of antibiotic therapy.
authors.affiliation: From the *Departments of Pediatrics, University of Thessaly, School of Health Sciences, Faculty of Medicine, General University Hospital, Larissa, Greece; †Univ Paris Diderot, Sorbonne Paris Cité; ‡AP-HP, Laboratoire de Microbiologie, Hôpital Robert-Debré, Paris, France; Departments of §Orthopedic Surgery; and ¶Radiology, University of Thessaly, School of Health Sciences, Faculty of Medicine, General University Hospital, Larissa, Greece.
authors.forename: Ioanna N
authors.initials: IN
authors.lastname: Grivea
authors.forename: Aspasia N
authors.initials: AN
authors.lastname: Michoula
authors.forename: Romain
authors.initials: R
authors.lastname: Basmaci
authors.forename: Zoe H
authors.initials: ZH
authors.lastname: Dailiana
authors.forename: George
authors.initials: G
authors.lastname: Tsimitselis
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bonacorsi
authors.forename: George A
authors.initials: GA
authors.lastname: Syrogiannopoulos
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001170:Arthritis, Infectious / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D006115:Greece; D006801:Humans; D007223:Infant; D017882:Kingella kingae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007719:Knee Joint / Q000382:microbiology / Q000473:pathology; D058885:Multilocus Sequence Typing; D016870:Neisseriaceae Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial