title: Genistein attenuates choroidal neovascularization.
abstract: Genistein is a dietary-derived flavonoid abundantly present in soybeans and known to possess various biological effects including anti-inflammation and anti-angiogenic activity. To investigate the effects of genistein on intraocular neovascularization, we used an animal model of laser-induced choroidal neovascularization (CNV). Male C57BL/6J mice were treated in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. CNV was induced by laser photocoagulation. The animals were fed a mixture diet containing 0.5% genistein or a control diet ad libitum for 7 days before laser photocoagulation and the treatment was continued until the end of the study. Seven days after laser injury, the size of CNV lesions was quantified. Retinal pigment epithelium (RPE)-choroid complex was also harvested 1 or 3 days after laser injury and the level of monocyte chemoattractant protein (MCP)-1, intercellular adhesion molecule (ICAM)-1, and matrix metalloproteinase (MMP)-9 were measured by enzyme-linked immunosorbent assay. Expression levels of Ets-1 and F4/80 were examined by real-time PCR. A significant decrease in CNV size was observed in animals treated with genistein (15441.9±1511.8 μm(2)) compared to control mice (21074.0±1940.7μm(2), P<.05). Genistein significantly reduced the protein level of MCP-1, ICAM-1, and MMP-9 in the RPE-choroid complex (P<.05). In addition, genistein suppressed the expression levels of Ets-1 and F4/80 (P<.05). The current data indicate the anti-angiogenic property of genistein during CNV formation.
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science; Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kinoshita
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science; Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan. Electronic address: nodako@med.hokudai.ac.jp.
authors.forename: Kousuke
authors.initials: K
authors.lastname: Noda
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science; Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tagawa
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science; Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Inafuku
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science; Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science; Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Fukuhara
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science; Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science; Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Ando
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science; Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Kanda
authors.affiliation: Laboratory of Ocular Cell Biology and Visual Science; Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Ishida
mesh_terms: D000818:Animals; D001483:Base Sequence; D020256:Choroidal Neovascularization / Q000201:enzymology / Q000378:metabolism / Q000517:prevention & control*; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D019833:Genistein / Q000494:pharmacology*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D050784:Proto-Oncogene Protein c-ets-1 / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C494042:Ets1 protein, mouse; D050784:Proto-Oncogene Protein c-ets-1; D019833:Genistein; D020780:Matrix Metalloproteinase 9
keywords: Age-related macular Degeneration; Choroidal neovascularization; Flavonoid; Genistein; Matrix metalloproteinase-9