title: Alternative mounting media for preservation of some protozoa.
abstract: Protozoa resistant stages are disintegrated when mounted in toluene-based media. To overcome such problem, three toluene-free mountants were tested on preserve Acanthamoeba spp and gregarines. Two commercial glues based on cyanoacrylate or trimethoxysilane were suitable for preserving both cysts and trophozoites. Hoyer's medium showed good results for mounting gregarine oocysts.
authors.affiliation: Laboratorio de Parasitología, Departamento de Biomedicina y Biotecnología, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain. Electronic address: angel.criado@uah.es.
authors.forename: A
authors.initials: A
authors.lastname: Criado-Fornelio
authors.affiliation: Laboratorio de Parasitología, Departamento de Biomedicina y Biotecnología, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Heredero-Bermejo
authors.affiliation: Laboratorio de Parasitología, Departamento de Biomedicina y Biotecnología, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Pérez-Serrano
mesh_terms: D000048:Acanthamoeba / Q000502:physiology*; D016782:Apicomplexa / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry*; D008853:Microscopy; D033661:Oocysts / Q000502:physiology; D011309:Preservation, Biological / Q000379:methods*; D033761:Spores, Protozoan / Q000502:physiology
chemical_list: D003470:Culture Media
keywords: Acanthamoeba; Cyanoacrylate; Gregarines; Hoyer's medium; Mountant; Trimethoxysilane