title: Molecular characterization of rice sphingosine-1-phosphate lyase gene OsSPL1 and functional analysis of its role in disease resistance response.
abstract: Our results indicate that overexpression of OsSPL1 in transgenic tobacco plants attenuated disease resistance and facilitated programmed cell death. Long-chain base phosphates including sphingosine-1-phosphate have been shown to act as signaling mediators in regulating programmed cell death (PCD) and stress responses in mammals. In the present study, we characterized a rice gene OsSPL1, encoding a putative sphingosine-1-phosphate lyase that is involved in metabolism of sphingosine-1-phosphate. Expression of OsSPL1 was down-regulated in rice plants after treatments with salicylic acid, benzothiadiazole and 1-amino cyclopropane-1-carboxylic acid, but was induced by infection with a virulent strain of Magnaporthe oryzae, the causal agent of rice blast disease. Transgenic tobacco lines with overexpression of OsSPL1 were generated and analyzed for the possible role of OsSPL1 in disease resistance response and PCD. The OsSPL1-overexpressing tobacco plants displayed increased susceptibility to infection of Pseudomonas syringae pv. tabaci (Pst), the causal agent of wildfire disease, showing severity of disease symptom and bacterial titers in inoculated leaves, and attenuated pathogen-induced expression of PR genes after infection of Pst as compared to the wild-type and vector-transformed plants. Higher level of cell death, as revealed by dead cell staining, leakage of electrolyte and expression of hypersensitive response indicator genes, was observed in the OsSPL1-overexpressing plants after treatment with fumonisin B1, a fungal toxin that induces PCD in plants. Our results suggest that OsSPL1 has different functions in regulating disease resistance response and PCD in plants.
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310058, People's Republic of China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Jin
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Hong
authors.forename: Yafen
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Ouyang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.forename: Fengming
authors.initials: F
authors.lastname: Song
authors.forename: Dayong
authors.initials: D
authors.lastname: Li
mesh_terms: D000446:Aldehyde-Lyases / Q000235:genetics / Q000378:metabolism*; D060467:Disease Resistance / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D044224:Pseudomonas syringae / Q000472:pathogenicity; D020156:Salicylic Acid / Q000494:pharmacology; D013830:Thiadiazoles / Q000494:pharmacology
chemical_list: D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole; D000446:Aldehyde-Lyases; C022649:sphingosine 1-phosphate lyase (aldolase); D020156:Salicylic Acid
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