title: Condensin suppresses recombination and regulates double-strand break processing at the repetitive ribosomal DNA array to ensure proper chromosome segregation during meiosis in budding yeast.
abstract: During meiosis, homologues are linked by crossover, which is required for bipolar chromosome orientation before chromosome segregation at anaphase I. The repetitive ribosomal DNA (rDNA) array, however, undergoes little or no meiotic recombination. Hyperrecombination can cause chromosome missegregation and rDNA copy number instability. We report here that condensin, a conserved protein complex required for chromosome organization, regulates double-strand break (DSB) formation and repair at the rDNA gene cluster during meiosis in budding yeast. Condensin is highly enriched at the rDNA region during prophase I, released at the prophase I/metaphase I transition, and reassociates with rDNA before anaphase I onset. We show that condensin plays a dual role in maintaining rDNA stability: it suppresses the formation of Spo11-mediated rDNA breaks, and it promotes DSB processing to ensure proper chromosome segregation. Condensin is unnecessary for the export of rDNA breaks outside the nucleolus but required for timely repair of meiotic DSBs. Our work reveals that condensin coordinates meiotic recombination with chromosome segregation at the repetitive rDNA sequence, thereby maintaining genome integrity.
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4370.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4370.
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4370 hyu@bio.fsu.edu.
authors.forename: Hong-Guo
authors.initials: HG
authors.lastname: Yu
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000705:Anaphase / Q000235:genetics; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics; D020090:Chromosome Segregation / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors; D042822:Genomic Instability / Q000235:genetics; D008540:Meiosis / Q000235:genetics; D008870:Microtubules / Q000378:metabolism; D046912:Multiprotein Complexes / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D008941:Spindle Apparatus / Q000378:metabolism; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D000251:Adenosine Triphosphatases; D018797:Cell Cycle Proteins; D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases; D046912:Multiprotein Complexes; D017346:Protein Serine-Threonine Kinases; D017027:Protein Tyrosine Phosphatases; D029701:Saccharomyces cerevisiae Proteins; C074860:CDC14 protein, S cerevisiae; C108312:condensin complexes; C081927:CDC5 protein, S cerevisiae; C518938:Spo11 protein, S cerevisiae; D000099257:Meiotic Recombination Protein SPO11
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