title: A micellar on-pathway intermediate step explains the kinetics of prion amyloid formation.
abstract: In a previous work by Alvarez-Martinez et al. (2011), the authors pointed out some fallacies in the mainstream interpretation of the prion amyloid formation. It appeared necessary to propose an original hypothesis able to reconcile the in vitro data with the predictions of a mathematical model describing the problem. Here, a model is developed accordingly with the hypothesis that an intermediate on-pathway leads to the conformation of the prion protein into an amyloid competent isoform thanks to a structure, called micelles, formed from hydrodynamic interaction. The authors also compare data to the prediction of their model and propose a new hypothesis for the formation of infectious prion amyloids.
authors.affiliation: CI2MA, Universidad de Concepción, Concepción, Chile.
authors.forename: Erwan
authors.initials: E
authors.lastname: Hingant
authors.affiliation: INSERM U710, Université Montpellier 2, Place E. Bataillon, 3eme étage, Montpellier, France.
authors.forename: Pascaline
authors.initials: P
authors.lastname: Fontes
authors.affiliation: Etablissement Confiné d'Expérimentation, Plateforme RAM, UMS 3426-BioCampus, Place E. Bataillon, UM2, Montpellier, France.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Alvarez-Martinez
authors.affiliation: Etablissement Confiné d'Expérimentation, Plateforme RAM, UMS 3426-BioCampus, Place E. Bataillon, UM2, Montpellier, France.
authors.forename: Jacques-Damien
authors.initials: JD
authors.lastname: Arnaud
authors.affiliation: Centre de Recherche sur les Pathogènes et Biologie pour la Santé, CPBS UMR5236, Université Montpellier 2, Place E. Bataillon, Montpellier, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Liautard
authors.affiliation: Université de Lyon, CNRS, Université Lyon 1, Institut Camille Jordan UMR5208, Villeurbanne, France; INRIA Team Dracula, Inria Center Grenoble Rhône-Alpes, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Pujo-Menjouet
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008823:Micelles; D008958:Models, Molecular; D011328:Prions / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D000682:Amyloid; D008823:Micelles; D011328:Prions
references.citation: Biophys Chem. 1999 Mar 29;77(2-3):139-52
references.pmid: 10326247
references.citation: Science. 2010 Feb 26;327(5969):1132-5
references.pmid: 20110469
references.citation: Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13363-83
references.pmid: 9811807
references.citation: Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):5959-63
references.pmid: 8327467
references.citation: J Mol Biol. 2006 Mar 31;357(3):833-41
references.pmid: 16466741
references.citation: Biochemistry. 2007 Jun 12;46(23):7045-53
references.pmid: 17503780
references.citation: Methods. 2011 Mar;53(3):208-13
references.pmid: 21176786
references.citation: J Mol Biol. 2002 Feb 1;315(5):1241-56
references.pmid: 11827491
references.citation: Biophys J. 2010 May 19;98(10):2236-45
references.pmid: 20483332
references.citation: Methods Enzymol. 1999;309:256-74
references.pmid: 10507029
references.citation: Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9741-6
references.pmid: 17535913
references.citation: Science. 2000 Aug 25;289(5483):1317-21
references.pmid: 10958771
references.citation: Biophys J. 2008 Jan 15;94(2):379-91
references.pmid: 17890392
references.citation: Annu Rev Biochem. 1997;66:385-407
references.pmid: 9242912
references.citation: J Theor Biol. 2006 Oct 7;242(3):598-606
references.pmid: 16753184
references.citation: Biochemistry. 2005 Feb 22;44(7):2339-48
references.pmid: 15709746
references.citation: Proteins. 2010 Jun;78(8):1909-25
references.pmid: 20229606
references.citation: Annu Rev Biochem. 2004;73:617-56
references.pmid: 15189155
references.citation: Biophys J. 2006 Jul 1;91(1):122-32
references.pmid: 16603497
references.citation: Biochim Biophys Acta. 2011 Oct;1814(10):1305-17
references.pmid: 21683809
references.citation: Biophys J. 2007 May 15;92(10):3448-58
references.pmid: 17325005
references.citation: PLoS Biol. 2004 Oct;2(10):e321
references.pmid: 15383837
references.citation: J Biol Chem. 1999 Dec 24;274(52):36859-65
references.pmid: 10601237
references.citation: Science. 1982 Apr 9;216(4542):136-44
references.pmid: 6801762
references.citation: Biochemistry. 2010 Sep 21;49(37):8169-76
references.pmid: 20718504