title: Changes in plasma amino acid profiles, growth performance and intestinal antioxidant capacity of piglets following increased consumption of methionine as its hydroxy analogue.
abstract: The aim of the present study was to determine whether early weaning-induced growth retardation could be attenuated by increased consumption of methionine as DL-methionine (DLM) or DL-2-hydroxy-4-methylthiobutyrate (HMTBA) in both lactating sows and weaned piglets. Therefore, diets containing DLM and HMTBA at 25% of the total sulphur-containing amino acids (AA) present in the control (CON) diet were fed to lactating sows and weaned piglets and their responses were evaluated. Compared with the CON diet-fed sows, the HMTBA diet-fed sows exhibited a tendency (P<0·10) towards higher plasma taurine concentrations and the DLM diet-fed sows had higher (P<0·05) plasma taurine concentrations, but lower (P<0·05) isoleucine concentrations. Suckling piglets in the HMTBA treatment group had higher (P<0·05) intestinal reduced glutathione (GSH) content, lower (P<0·05) oxidised glutathione (GSSG):GSH ratio, and higher (P<0·05) plasma cysteine and glutathione peroxidase (GPx) activity than those in the CON and DLM treatment groups. The feed intake (P<0·05) and body weight of piglets averaged across post-weaning (PW) days were higher (P< 0·05) in the HMTBA treatment group than in the DLM treatment group and were higher (P<0·05) and tended (P<0·10) to be higher, respectively, in the HMTBA treatment group than in the CON treatment group. Increased (P<0·05) GSSG content and GSSG:GSH ratio and down-regulated (P<0·05) expression of nutrient transport genes were observed in the jejunum of piglets on PW day 7 than on PW day 0. On PW day 14, the HMTBA diet-fed piglets had higher (P<0·05) intestinal GSH content than the CON diet-fed piglets and higher (P<0·05) plasma GPx activity, villus height and goblet cell numbers than the CON diet- and DLM diet-fed piglets. In conclusion, early weaning-induced growth retardation appears to be attenuated through changes in plasma AA profiles and elevation of growth performance and intestinal antioxidant capacity in piglets following increased consumption of methionine as HMTBA.
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wan
authors.affiliation: Adisseo France S.A.S., CERN,Commentry,France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Mercier
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Caimei
authors.initials: C
authors.lastname: Wu
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Xiuqun
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Lianqiang
authors.initials: L
authors.lastname: Che
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Shengyu
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Tian
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: De
authors.initials: D
authors.lastname: Wu
authors.affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University,Ya'an625014,People's Republic of China.
authors.forename: Zhengfeng
authors.initials: Z
authors.lastname: Fang
mesh_terms: D000596:Amino Acids / Q000097:blood* / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002681:China; D003433:Crosses, Genetic; D004032:Diet / Q000662:veterinary*; D002149:Energy Intake; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D007774:Lactation; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D008715:Methionine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D018384:Oxidative Stress; D011247:Pregnancy; D034421:Sus scrofa / Q000097:blood / Q000254:growth & development* / Q000378:metabolism; D013654:Taurine / Q000097:blood / Q000378:metabolism; D014886:Weaning; D015430:Weight Gain
chemical_list: D000596:Amino Acids; D013654:Taurine; C008391:alpha-hydroxy-gamma-methylmercaptobutyric acid; D008715:Methionine; D005978:Glutathione