title: Species identification in the genus Saprolegnia (Oomycetes): defining DNA-based molecular operational taxonomic units.
abstract: The lack of a robust taxonomy in the genus Saprolegnia is leading to the presence of incorrectly named isolates in culture collections and of an increasing number of misassigned sequences in DNA databases. Accurate species delimitation is critical for most biological disciplines. A recently proposed approach to solve species delimitation (taxonomic diagnosis system) of difficult organisms is the definition of molecular operational taxonomic units (MOTUs). We have used 961 sequences of nrDNA ITS from culture collections (461 sequences) and GenBank (500 sequences), to perform phylogenetic and clustering optimization analyses. As result, we have identified 29 DNA-based MOTUs in agreement with phylogenetic studies. The resulting molecular clusters support the validity of 18 species of Saprolegnia and identify 11 potential new ones. We have also listed a number of incorrectly named isolates in culture collections, misassigned species names to GenBank sequences, and reference sequences for the species. We conclude that GenBank represents the main source of errors for identifying Saprolegnia species since it possesses sequences with misassigned names and also sequencing errors. The presented taxonomic diagnosis system might help setting the basis for a suitable identification of species in this economically important genus.
authors.affiliation: Departamento de Micología, Real Jardín Botánico-CSIC, Plaza Murillo 2, 28014 Madrid, Spain.
authors.forename: Jose Vladimir
authors.initials: JV
authors.lastname: Sandoval-Sierra
authors.affiliation: Departamento de Micología, Real Jardín Botánico-CSIC, Plaza Murillo 2, 28014 Madrid, Spain.
authors.forename: María P
authors.initials: MP
authors.lastname: Martín
authors.affiliation: Departamento de Micología, Real Jardín Botánico-CSIC, Plaza Murillo 2, 28014 Madrid, Spain. Electronic address: dieguez@rjb.csic.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Diéguez-Uribeondo
mesh_terms: D016000:Cluster Analysis; D048028:DNA, Algal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D044762:Saprolegnia / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D048028:DNA, Algal; D021903:DNA, Ribosomal Spacer
keywords: GenBank; ITS; Pathogens; Phylogeny; Taxonomy