title: Effect of γ-aminobutyric acid on digestive enzymes, absorption function, and immune function of intestinal mucosa in heat-stressed chicken.
abstract: To explore the effect of dietary γ-aminobutyric acid (GABA) on digestive enzyme activity, absorption function and immune function of intestinal mucosa in heat-stressed Wenchang chicken were studied. One-day-old male Wenchang chickens were randomly divided into a control group (CK), heat stress group (HS), and GABA+HS group. The chickens from the GABA+HS group were administered with 0.2 mL of GABA solution daily. Chickens from HS and GABA+HS groups were subjected to heat stress treatment at 40 ± 0.5°C for 2 h during 1300 to 1500 h every day. Blood was drawn and 0.5 cm-long duodenum, jejunum, and ileum were collected from the chickens on d 3, 5, 7, 9, 12, and 15. Results showed that the activity of Ca²⁺-Mg²⁺-adenosine triphosphatase (ATPase), Na⁺-K⁺-ATPase, maltase, sucrase, and alkaline phosphatase, the contents of secretory IgA, glutathione, and d-xylose, and the number of lymphocytes in HS group were significantly lower than those in the CK group. Among them, some were rescued after the treatment of GABA as the time extension. For maltase, d-xylose, alkaline phosphatase, and Na⁺-K⁺-ATPase, it required 5 to 7 d for achieving the significant effect. For sucrase, 12 d for the alleviation effect was required. In the case of other parameters, no alleviation was observed during the whole period of the study. We have concluded that HS can inhibit the activity of digestive enzymes and reduce absorption and immune functions of intestinal mucosa. γ-Aminobutyric acid can effectively alleviate these inhibitory effects.
authors.affiliation: Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, Hainan Normal University, Haikou 571158, China zh.chen@hainnu.edu.cn.
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, Hainan Normal University, Haikou 571158, China.
authors.forename: J
authors.initials: J
authors.lastname: Xie
authors.affiliation: Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, Hainan Normal University, Haikou 571158, China.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, Hainan Normal University, Haikou 571158, China.
authors.forename: J
authors.initials: J
authors.lastname: Tang
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology* / Q000378:metabolism*; D018869:Heat-Shock Response; D006358:Hot Temperature; D007113:Immunity, Innate; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000201:enzymology; D008297:Male; D011897:Random Allocation; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005680:gamma-Aminobutyric Acid
keywords: absorption; digestive enzyme; heat stress; immune function; γ-aminobutyric acid