title: Genetic polymorphism of 13 non-CODIS STR loci in three national populations from China.
abstract: The aim of this study was to investigate a 13 non-CODIS STR loci database using three national populations from China. A new multiplex PCR system that simultaneously amplified 13 loci in the same PCR reaction was developed. This multiplex system included the 13 STR markers (D3S2402, D3S2452, D3S1766, D3S4554, D3S2388, D3S3051, D3S3053, D4S2364, D4S2404, AC001348A, AC001348B, D17S975, and D17S1294), which were successfully analyzed by using 441 DNA samples from three national populations in China (154 Mongol, 177 Kazakh, and 110 Uigur). Allele frequencies and mutation rates of the 13 non-CODIS STR loci were investigated. A total of 4-10 alleles at each locus were observed and altogether 84, 88, and 87 alleles for the all selected loci were found in the Mongol, Kazakh, and Uigur, respectively. Eight mutations were detected from the 13 selected loci in 9880 meioses in kinship cases. These results indicate that this multiplex system may provide significant polymorphic information for kinship testing and relationship investigations.
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China; Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, P. R. China.
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Liu
authors.forename: Kai-Kai
authors.initials: KK
authors.lastname: Huang
authors.forename: Ye-Da
authors.initials: YD
authors.lastname: Wu
authors.forename: Hu
authors.initials: H
authors.lastname: Zhao
authors.forename: Cheng-Tao
authors.initials: CT
authors.lastname: Li
authors.forename: De-Jian
authors.initials: DJ
authors.lastname: Lu
mesh_terms: D044466:Asian People / Q000235:genetics*; D002681:China; D030541:Databases, Genetic; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009154:Mutation / Q000235:genetics; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers
keywords: Genetic polymorphism; Haplotype; Linkage; Multiplex PCR; STR