title: Photoacoustic microscopy using ultrashort pulses with two different pulse durations.
abstract: We propose photoacoustic microscopy using ultrashort pulses with two different pulse durations in the range from femtoseconds to picoseconds. The subtraction of images for longer-pulse excitation from those for shorter-pulse excitation extracts two-photon photoacoustic images effectively, based on observation that the intensity ratio of two-photon to one-photon absorption-induced photoacoustic signals depends on the pulse duration in the same manner as the intensity ratio of two-photon and one-photon fluorescence signals. Two-photon photoacoustic microscopy using this subtraction method enables precise observation of the cross-sections of silicone hollows filled with the mixture of one-photon and two-photon absorption solutions.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Yamaoka
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Harada
authors.forename: Masaaki
authors.initials: M
authors.lastname: Sakakura
authors.forename: Takeo
authors.initials: T
authors.lastname: Minamikawa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Nishino
authors.forename: Seiji
authors.initials: S
authors.lastname: Maehara
authors.forename: Shujiro
authors.initials: S
authors.lastname: Hamano
authors.forename: Hideo
authors.initials: H
authors.lastname: Tanaka
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Takamatsu