title: Coal fly ash ceramics: preparation, characterization, and use in the hydrolysis of sucrose.
abstract: Coal ash is a byproduct of mineral coal combustion in thermal power plants. This residue is responsible for many environmental problems because it pollutes soil, water, and air. Thus, it is important to find ways to reuse it. In this study, coal fly ash, obtained from the Presidente Médici Thermal Power Plant, was utilized in the preparation of ceramic supports for the immobilization of the enzyme invertase and subsequent hydrolysis of sucrose. Coal fly ash supports were prepared at several compaction pressures (63.66-318.30 MPa) and sintered at 1200°C for 4 h. Mineralogical composition (by X-ray diffraction) and surface area were studied. The ceramic prepared with 318.30 MPa presented the highest surface area (35 m(2)/g) and amount of immobilized enzyme per g of support (76.6 mg/g). In assays involving sucrose inversion, it showed a high degree of hydrolysis (around 81%) even after nine reuses and 30 days' storage. Therefore, coal fly ash ceramics were demonstrated to be a promising biotechnological alternative as an immobilization support for the hydrolysis of sucrose.
authors.affiliation: Computer Engineering, Federal University of Ceará, 62010-560 Sobral, CE, Brazil.
authors.forename: Ricardo Pires
authors.initials: RP
authors.lastname: dos Santos
authors.affiliation: Institute of Chemistry and Geosciences, Federal University of Pelotas, 96160-000 Pelotas, RS, Brazil.
authors.forename: Jorge
authors.initials: J
authors.lastname: Martins
authors.affiliation: Department of Molecular Biology, Federal University of Paraíba, 58059-900 João Pessoa, PB, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Gadelha
authors.affiliation: Laboratory of Biologically Active Molecules, Federal University of Ceará, 60440-970 Fortaleza, CE, Brazil.
authors.forename: Benildo
authors.initials: B
authors.lastname: Cavada
authors.affiliation: Laboratory of Theoretical, Computational and Experimental Biophysics, Federal Rural University of Pernambuco, 52171-900 Recife, PE, Brazil.
authors.forename: Alessandro Victor
authors.initials: AV
authors.lastname: Albertini
authors.affiliation: Integrated Laboratory of Biomolecules, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Francisco
authors.initials: F
authors.lastname: Arruda
authors.affiliation: Laboratory of Biologically Active Molecules, Federal University of Ceará, 60440-970 Fortaleza, CE, Brazil ; Integrated Laboratory of Biomolecules, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Mayron
authors.initials: M
authors.lastname: Vasconcelos
authors.affiliation: Integrated Laboratory of Biomolecules, Federal University of Ceará, 60430-160 Fortaleza, CE, Brazil.
authors.forename: Edson
authors.initials: E
authors.lastname: Teixeira
authors.affiliation: Computer Engineering, Federal University of Ceará, 62010-560 Sobral, CE, Brazil.
authors.forename: Francisca
authors.initials: F
authors.lastname: Alves
authors.affiliation: Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, 50780-901 Recife, PE, Brazil.
authors.forename: José
authors.initials: J
authors.lastname: Lima Filho
authors.affiliation: Department of Physics, Federal University of Ceará, 60455-900 Fortaleza, CE, Brazil.
authors.forename: Valder
authors.initials: V
authors.lastname: Freire
mesh_terms: D002516:Ceramics / Q000737:chemistry*; D060729:Coal Ash / Q000737:chemistry*; D006868:Hydrolysis; D010316:Particle Size; D013395:Sucrose / Q000737:chemistry; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D060729:Coal Ash; D013395:Sucrose
references.citation: J Hazard Mater. 2010 Sep 15;181(1-3):82-90
references.pmid: 20546994
references.citation: J Biotechnol. 2008 Feb 1;133(3):403-11
references.pmid: 18035441
references.citation: Biomacromolecules. 2007 Jun;8(6):1775-89
references.pmid: 17444679
references.citation: Biotechnol Adv. 2009 Jul-Aug;27(4):311-22
references.pmid: 19472508
references.citation: Phytochemistry. 2010 Oct;71(14-15):1610-4
references.pmid: 20696445
references.citation: Prog Energy Combust Sci. 2010 Aug 1;36(4):
references.pmid: 24223466
references.citation: J Environ Manage. 2010 Jan-Feb;91(3):603-17
references.pmid: 19914766