title: Reciprocal interaction between fish TGF-β1 and IL-1β is responsible for restraining IL-1β signaling activity in grass carp head kidney leukocytes.
abstract: In the present study, we found that recombinant grass carp IL-1β (rgcIL-1β) simultaneously up-regulated grass carp IL-1β (gcIL-1β) and TGF-β1 (gcTGF-β1) expression via NF-κB and MAPK signaling in grass carp head kidney leukocytes (HKLs), promoting us to clarify whether TGF-β1 is an effective antagonist in IL-1β expression and activity. Our results showed that a stimulation of gcIL-1β on its own expression was noted within 6 h, but gcTGF-β1 neutralizing antibody prolonged gcIL-1β autostimulation up to 12 h, indicating a possible inhibitory role of gcTGF-β1 in regulating gcIL-1β effect. This notion was reinforced by the fact that recombinant grass carp TGF-β1 (rgcTGF-β1) could impede rgcIL-1β-induced gcIL-1β gene expression and secretion in a reciprocal manner. Further studies revealed that rgcTGF-β1 was able to attenuate rgcIL-1β-induced mRNA expression of its own receptor signaling molecules and the activation of NF-κB. By contrast, rgcIL-1β significantly amplified rgcTGF-β1-mediated gcTGF-β1 type I receptor (ALK5) expression and Smad2 phosphorylation in the same cell model. Taken together, these data shed light on an intrinsic mechanism for controlling inflammatory response by the reciprocal interaction between TGF-β1 and IL-1β in teleost.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: He
authors.initials: H
authors.lastname: Wei
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Anying
authors.initials: A
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Linyong
authors.initials: L
authors.lastname: Du
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. Electronic address: zhouhongzh@uestc.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000494:pharmacology; D002347:Carps; D025461:Feedback, Physiological; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D060226:Head Kidney / Q000166:cytology / Q000276:immunology*; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D007962:Leukocytes / Q000166:cytology / Q000276:immunology*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000276:immunology; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D010766:Phosphorylation / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D051899:Smad2 Protein / Q000235:genetics / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D029941:Fish Proteins; D053583:Interleukin-1beta; D016328:NF-kappa B; D034741:RNA, Small Interfering; D018125:Receptors, Transforming Growth Factor beta; D051899:Smad2 Protein; D053773:Transforming Growth Factor beta1; D017346:Protein Serine-Threonine Kinases; D020928:Mitogen-Activated Protein Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I
keywords: Grass carp; Head kidney leukocytes; IL-1β; Reciprocal interaction; Signaling pathway; TGF-β1