title: Mammalian cellular culture models of Trypanosoma cruzi infection: a review of the published literature.
abstract: Cellular culture infection with Trypanosoma cruzi is a tool used to dissect the biological mechanisms behind Chagas disease as well as to screen potential trypanocidal compounds. Data on these models are highly heterogeneous, which represents a challenge when attempting to compare different studies. The purpose of this review is to provide an overview of the cell culture infectivity assays performed to date. Scientific journal databases were searched for articles in which cultured cells were infected with any Trypanosoma cruzi strain or isolate regardless of the study's goal. From these articles the cell type, parasite genotype, culture conditions and infectivity results were extracted. This review represents an initial step toward the unification of infectivity model data. Important differences were detected when comparing the pathophysiology of Chagas disease with the experimental conditions used in the analyzed studies. While Trypanosoma cruzi preferentially infects stromal cells in vivo, most of the assays employ epithelial cell lines. Furthermore, the most commonly used parasite strain (Tulahuen-TcVI) is associated with chagasic cardiomyopathy only in the Southern Cone of South America. Suggestions to overcome these discrepancies include the use of stromal cell lines and parasite genotypes associated with the known characteristics of the natural history of Chagas disease.
authors.affiliation: Grupo de Ciencias Básicas Médicas, Facultad de Medicina, Universidad de los Andes, Bogotá, DC, Colombia.
authors.forename: Gabriel Alberto
authors.initials: GA
authors.lastname: Duran-Rehbein
authors.affiliation: Grupo de Ciencias Básicas Médicas, Facultad de Medicina, Universidad de los Andes, Bogotá, DC, Colombia.
authors.forename: Juan Camilo
authors.initials: JC
authors.lastname: Vargas-Zambrano
authors.affiliation: Grupo de Inmunobiología y Biología Celular, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, DC, Colombia.
authors.forename: Adriana
authors.initials: A
authors.lastname: Cuéllar
authors.affiliation: Laboratorio de Parasitología Molecular, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá DC, Colombia.
authors.forename: Concepción Judith
authors.initials: CJ
authors.lastname: Puerta
authors.affiliation: Grupo de Ciencias Básicas Médicas, Facultad de Medicina, Universidad de los Andes, Bogotá, DC, Colombia.
authors.forename: John Mario
authors.initials: JM
authors.lastname: Gonzalez
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D002478:Cells, Cultured / Q000469:parasitology*; D002598:Chagas Cardiomyopathy / Q000469:parasitology; D014355:Chagas Disease / Q000469:parasitology*; D004847:Epithelial Cells / Q000469:parasitology; D005838:Genotype; D000882:Haplorhini; D006801:Humans; D007668:Kidney / Q000166:cytology; D008322:Mammals; D010274:Parasitology / Q000379:methods*; D017154:Stromal Cells / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity
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