title: Acinetobacter apis sp. nov., isolated from the intestinal tract of a honey bee, Apis mellifera.
abstract: A novel Gram-negative, obligate aerobic, non-motile, and both coccobacillus- and bacillus-shaped bacterium, designated strain HYN18(T), was isolated from the intestinal tract of a honey bee (Apis mellifera). The isolate was oxidase-negative and catalase-positive. Strain HYN18(T) showed optimum growth at 25°C, pH 6-7, and in the presence of 1% (w/v) NaCl in trypticase soy broth medium. The isolate was negative for hydrolyses of starch, casein, gelatin and urea, indole production from tryptone and hemolysis on sheep blood agar. A phylogenetic analysis based on the 16S rRNA gene and rpoB gene sequence showed that strain HYN18(T) was most closely related to Acinetobacter nectaris SAP 763.2(T) and A. boissieri SAP 284.1(T) with 98.3% and 98.1% similarity (16S rRNA gene), respectively, and 84.4% similarity with Acinetobacter nectaris SAP 763.2(T) (rpoB gene). The major cellular fatty acids were summed features 3 (comprising C16:1ω7c /C16:1ω6c ), C12:0 and C16:0. The main isoprenoid quinone was ubiquinone-9 (Q-9). The polar lipids of strain HYN18(T) were phosphatidylethanolamine, three unidentified lipids, an unidentified phospholipid and an unidentified glycolipid. The DNA G+C content was 40.6 mol%. DNA-DNA hybridization experiments indicated less than 33 ± 10% relatedness to the closest phylogenetic species, Acinetobacter nectaris SAP 763.2(T). Thus, the phenotypic, phylogenetic and genotypic analyses indicate that strain HYN18(T) is a novel species within the genus Acinetobacter, for which the name Acinetobacter apis is proposed. The type strain is HYN18(T) (=KACC 16906(T) =JCM 18575(T)).
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, 130-701, Republic of Korea.
authors.forename: Pil Soo
authors.initials: PS
authors.lastname: Kim
authors.forename: Na-Ri
authors.initials: NR
authors.lastname: Shin
authors.forename: Joon Yong
authors.initials: JY
authors.lastname: Kim
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Yun
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Hyun
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Bae
mesh_terms: D000150:Acinetobacter / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001516:Bees / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis*; D005838:Genotype; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010714:Phosphatidylethanolamines / Q000302:isolation & purification; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030571:ubiquinone 9
references.citation: Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):716-721
references.pmid: 22140171
references.citation: J Microbiol. 2013 Jun;51(3):301-4
references.pmid: 23812809
references.citation: J Microbiol Methods. 2008 Dec;75(3):523-30
references.pmid: 18782592
references.citation: Appl Environ Microbiol. 2005 Mar;71(3):1373-86
references.pmid: 15746340
references.citation: Int J Syst Evol Microbiol. 2009 Jan;59(Pt 1):118-24
references.pmid: 19126734
references.citation: Ann Inst Pasteur (Paris). 1954 Jan;86(1):118-20
references.pmid: 13158938
references.citation: J Microbiol. 2008 Aug;46(4):396-401
references.pmid: 18758729
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Aug 25;823(1):26-36
references.pmid: 15990370
references.citation: Int J Syst Bacteriol. 1997 Apr;47(2):590-2
references.pmid: 9103655
references.citation: J Microbiol. 2012 Oct;50(5):735-45
references.pmid: 23124740
references.citation: J Bacteriol. 1936 Jun;31(6):575-80
references.pmid: 16559914
references.citation: Appl Environ Microbiol. 2000 Aug;66(8):3376-80
references.pmid: 10919794
references.citation: Int J Syst Evol Microbiol. 2011 Dec;61(Pt 12):2837-2843
references.pmid: 21239566
references.citation: J Mol Evol. 1981;17(6):368-76
references.pmid: 7288891
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Int J Syst Evol Microbiol. 2012 Dec;62(Pt 12):2883-2890
references.pmid: 22247213
references.citation: Int J Syst Evol Microbiol. 2000 May;50 Pt 3:1297-1303
references.pmid: 10843075
references.citation: Environ Microbiol. 2002 Nov;4(11):770-3
references.pmid: 12460286
references.citation: Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1532-1539
references.pmid: 22904213
references.citation: BMC Ecol. 2006 Mar 21;6:4
references.pmid: 16551367
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: PLoS Biol. 2007 Jun;5(6):e168
references.pmid: 17564497
references.citation: J Clin Microbiol. 2006 Mar;44(3):827-32
references.pmid: 16517861
references.citation: Trends Ecol Evol. 2005 May;20(5):238-44
references.pmid: 16701375
references.citation: Int J Syst Evol Microbiol. 2003 Sep;53(Pt 5):1563-1567
references.pmid: 13130049
references.citation: J Clin Microbiol. 1988 May;26(5):831-6
references.pmid: 3384908
references.citation: Microbiol Rev. 1981 Jun;45(2):316-54
references.pmid: 7022156
references.citation: Appl Environ Microbiol. 2005 Dec;71(12):8825-35
references.pmid: 16332879
references.citation: Science. 2007 Oct 12;318(5848):283-7
references.pmid: 17823314
references.citation: FEMS Microbiol Lett. 1992 Dec 15;100(1-3):59-65
references.pmid: 1282488
references.citation: Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W5-9
references.pmid: 18440982
references.citation: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references.pmid: 7984417
references.citation: Microb Drug Resist. 2002 Winter;8(4):261-5
references.pmid: 12523622
references.citation: Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):953-963
references.pmid: 12892111