title: Regional and age-related differences in GAD67 expression of parvalbumin- and calbindin-expressing neurons in the rhesus macaque auditory midbrain and brainstem.
abstract: Neurons expressing the calcium binding proteins (CaBPs) parvalbumin (PV) and calbindin (CB) have shown age-related density changes throughout the ascending auditory system of both rodents and macaque monkeys. In the cerebral cortex, neurons expressing these CaBPs express markers of γ-aminobutyric acidergic neurotransmission, such as GAD67, and have well-understood physiological response properties. Recent evidence suggests that, in the rodent auditory brainstem, CaBP-containing cells do not express GAD67. It is unknown whether PV- and CB-containing cells in subcortical auditory structures of macaques similarly do not express GAD67, and a better understanding of the neurotransmission of neurons expressing these proteins is necessary for understanding the age-related changes in their density throughout the macaque auditory system. This was investigated with immunofluorescent double-labeling techniques to coregister PV- and CB-expressing neurons with GAD67 in the superior olivary complex and the inferior colliculus of young and aged rhesus macaques. The proportions of GAD67-expressing PV- and CB-positive neurons were computed with unbiased sampling techniques. Our results indicate that between 42% and 62% of PV- and CB-positive neurons in the auditory brainstem and midbrain express GAD67, which is significantly less than in the cerebrum. In general, fewer PV(+) neurons and more CB(+) neurons expressed GAD67 as a function of age. These results demonstrate that the inhibitory molecular profile of PV- and CB-expressing neurons can change with age in subcortical auditory structures and that these neurons are distinct from the well-described inhibitory interneurons that express these proteins in the cerebral cortex.
authors.affiliation: Center for Neuroscience, University of California at Davis, Davis, California, 95616; Evelyn F. McKnight Brain Institute, University of Arizona, Tucson, Arizona, 85721.
authors.forename: D T
authors.initials: DT
authors.lastname: Gray
authors.forename: J R
authors.initials: JR
authors.lastname: Engle
authors.forename: M L
authors.initials: ML
authors.lastname: Rudolph
authors.forename: G H
authors.initials: GH
authors.lastname: Recanzone
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D064026:Calbindins / Q000378:metabolism; D002452:Cell Count; D005260:Female; D005455:Fluorescent Antibody Technique; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D007245:Inferior Colliculi / Q000033:anatomy & histology / Q000378:metabolism*; D008253:Macaca mulatta / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism; D065832:Superior Olivary Complex / Q000378:metabolism*
chemical_list: D064026:Calbindins; D010320:Parvalbumins; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
keywords: GAD67; auditory midbrain; brainstem; calbindin; parvalbumin; rhesus macaque
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