title: Production of silver nanoparticles with strong and stable antimicrobial activity against highly pathogenic and multidrug resistant bacteria.
abstract: OBJECTIVE
  To synthesize, characterize, and analyze antimicrobial activity of AgNPs of Escherichia hermannii (SHE), Citrobacter sedlakii (S11P), and Pseudomonas putida (S5).
  
  
  METHODS
  The synthesized AgNPs were examined using ultraviolet-visible spectroscopy (UV-vis) and, zeta potential, and the size and the morphology obtained from the three different isolates were also confirmed by TEM.
  
  
  RESULTS
  Among the three isolates tested, SHE showed the best antimicrobial activity due to the presence of small (4-12 nm) and stable (-22 mV) AgNPs. Stability of AgNPs was also investigated and found to be dependent on the nature of isolates.
  
  
  CONCLUSIONS
  Produced AgNPs showed particle stability and antimicrobial efficacy up to 90 days of production. Our AgNPs exhibited greater antimicrobial activity compared with gentamicin against P. aeruginosa isolates and vancomycin against S. aureus and MRSA isolates at very low concentration (0.0002 mg per Microliters).
authors.affiliation: Strategic Center for Diabetes Research, King Saud University, P.O. Box 18397, Riyadh 11415, Saudi Arabia.
authors.forename: Amr T M
authors.initials: AT
authors.lastname: Saeb
authors.affiliation: The National Nanotechnology Center, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi Arabia.
authors.forename: Ahmad S
authors.initials: AS
authors.lastname: Alshammari
authors.affiliation: Strategic Center for Diabetes Research, King Saud University, P.O. Box 18397, Riyadh 11415, Saudi Arabia.
authors.forename: Hessa
authors.initials: H
authors.lastname: Al-Brahim
authors.affiliation: Strategic Center for Diabetes Research, King Saud University, P.O. Box 18397, Riyadh 11415, Saudi Arabia ; University Diabetes Center, King Saud University, P.O. Box 18397, Riyadh 11415, Saudi Arabia.
authors.forename: Khalid A
authors.initials: KA
authors.lastname: Al-Rubeaan
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D005839:Gentamicins / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D005839:Gentamicins; D012834:Silver; D014640:Vancomycin
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