title: Chondrogenic differentiation of human adipose mesenchimal stem cells: influence of a biomimetic gelatin genipin crosslinked porous scaffold.
abstract: Human adipose derived stem cells have shown chondrogenic differentiation potential in cartilage tissue engineering in combination with biomimetic materials. In this study, the chondrogenic potential of a porous gelatin based scaffold genipin (GNP) crosslinked was investigated in human mesenchymal stem cells obtained from adipose tissue. Cells were cultured up to 4 weeks on the scaffold and on monolayer, MTT assay was performed to evaluate cell viability, light, and transmission electron microscopy were carried out to demonstrate cell proliferation, scaffold adhesion, and cell colonization inside the porous architecture of the biomaterial. The expression of chondrogenic markers such as SOX9, collagen type II, aggregan, and versican was investigated by Real Time PCR. Results showed an high cell viability, adhesion, and colonization of the scaffold. Real Time PCR data demonstrated an upregulation of all the chondrogenic markers analyzed. In conclusion, 3D gelatin GNP crosslinked porous scaffold provides an improved environment for chondrogenic differentiation of stem cells compared with cell monolayer culture system.
authors.affiliation: Department for Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, 40126, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Focaroli
authors.forename: Gabriella
authors.initials: G
authors.lastname: Teti
authors.forename: Viviana
authors.initials: V
authors.lastname: Salvatore
authors.forename: Sandra
authors.initials: S
authors.lastname: Durante
authors.forename: Monica Mattioli
authors.initials: MM
authors.lastname: Belmonte
authors.forename: Roberto
authors.initials: R
authors.lastname: Giardino
authors.forename: Antonio
authors.initials: A
authors.lastname: Mazzotti
authors.forename: Adriana
authors.initials: A
authors.lastname: Bigi
authors.forename: Mirella
authors.initials: M
authors.lastname: Falconi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D053674:Aggrecans / Q000096:biosynthesis; D040761:Biomimetic Materials; D002454:Cell Differentiation / Q000502:physiology*; D020219:Chondrogenesis / Q000502:physiology*; D024043:Collagen Type II / Q000096:biosynthesis; D005780:Gelatin; D006801:Humans; D039823:Iridoids; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D060888:Real-Time Polymerase Chain Reaction; D055758:SOX9 Transcription Factor / Q000096:biosynthesis; D054457:Tissue Scaffolds; D053675:Versicans / Q000096:biosynthesis
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; D039823:Iridoids; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C508322:VCAN protein, human; D053675:Versicans; D005780:Gelatin; C007834:genipin
keywords: chondrogenic markers; tissue engineering; ultrastructure