title: The transcriptional activators AraR and XlnR from Aspergillus niger regulate expression of pentose catabolic and pentose phosphate pathway genes.
abstract: The pentose catabolic pathway (PCP) and the pentose phosphate pathway (PPP) are required for the conversion of pentose sugars in fungi and are linked via d-xylulose-5-phosphate. Previously, it was shown that the PCP is regulated by the transcriptional activators XlnR and AraR in Aspergillus niger. Here we assessed whether XlnR and AraR also regulate the PPP. Expression of two genes, rpiA and talB, was reduced in the ΔaraR/ΔxlnR strain and increased in the xylulokinase negative strain (xkiA1) on d-xylose and/or l-arabinose. Bioinformatic analysis of the 1 kb promoter regions of rpiA and talB showed the presence of putative XlnR binding sites. Combining all results in this study, it strongly suggests that these two PPP genes are under regulation of XlnR in A. niger.
authors.affiliation: Microbiology & Kluyver Centre for Genomics of Industrial Fermentation, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands; CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CY, Utrecht, The Netherlands. Electronic address: e.battaglia@cbs.knaw.nl.
authors.forename: Evy
authors.initials: E
authors.lastname: Battaglia
authors.affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CY, Utrecht, The Netherlands. Electronic address: m.zhou@cbs.knaw.nl.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Microbiology & Kluyver Centre for Genomics of Industrial Fermentation, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands; CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CY, Utrecht, The Netherlands. Electronic address: r.devries@cbs.knaw.nl.
authors.forename: Ronald P
authors.initials: RP
authors.lastname: de Vries
mesh_terms: D001234:Aspergillus niger / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D010427:Pentose Phosphate Pathway; D010429:Pentoses / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010429:Pentoses; D015534:Trans-Activators
keywords: AraR; Aspergillus niger; Pentose phosphate pathway; Regulation of gene expression; XlnR