title: Levels of circulating anti-muscarinic and anti-adrenergic antibodies and their effect on cardiac arrhythmias and dysautonomia in murine models of Chagas disease.
abstract: SUMMARY Antibodies (Ab) recognizing G-protein coupled receptors, such as β 1 and β 2 adrenergic (anti-β 1-AR and anti-β 2-AR, respectively) and muscarinic cholinergic receptors (anti-M2-CR) may contribute to cardiac damage, however their role in chronic chagasic cardiomyopathy is still controversial. We describe that Trypanosoma cruzi-infected C3H/He mice show increased P and QRS wave duration, and PR and QTc intervals, while the most significant ECG alterations in C57BL/6 are prolonged P wave and PR interval. Echocardiogram analyses show right ventricle dilation in infected animals of both mouse lineages. Analyses of heart rate variability (HRV) in chronically infected C3H/He mice show no alteration of the evaluated parameters, while C57BL/6 infected mice display significantly lower values of HRV components, suggesting autonomic dysfunction. The time-course analysis of anti-β 1-AR, anti-β 2-AR and anti-M2-CR Ab titres in C3H/He infected mice indicate that anti-β 1-AR Ab are detected only in the chronic phase, while anti-β 2-AR and anti-M2-CR are observed in the acute phase, diminish at 60 dpi and increase again in the chronic phase. Chronically infected C57BL/6 mice presented a significant increase in only anti-M2-CR Ab titres. Furthermore, anti-β 1-AR, anti-β 2-AR and anti-M2-CR, exhibit significantly higher prevalence in chronically T. cruzi-infected C3H/He mice when compared with C57BL/6. These observations suggest that T. cruzi infection leads to host-specific cardiac electric alterations.
authors.affiliation: Laboratório de Investigação Cardiovascular,Instituto Oswaldo Cruz,Fiocruz, Rio de Janeiro, RJ,Brazil.
authors.forename: Anissa
authors.initials: A
authors.lastname: Daliry
authors.affiliation: Laboratório de Biologia das Interações,Instituto Oswaldo Cruz,Fiocruz, Rio de Janeiro, RJ,Brazil.
authors.forename: Isabela Resende
authors.initials: IR
authors.lastname: Pereira
authors.affiliation: Laboratório de Eletrofisiologia Cardíaca,Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, UFRJ,Rio de Janeiro,Brazil.
authors.forename: Pedro Paulo
authors.initials: PP
authors.lastname: Pereira-Junior
authors.affiliation: Laboratório de Cardiologia Celular e Molecular,Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, UFRJ,Rio de Janeiro,Brazil.
authors.forename: Isalira Peroba
authors.initials: IP
authors.lastname: Ramos
authors.affiliation: Laboratório de Biologia das Interações,Instituto Oswaldo Cruz,Fiocruz, Rio de Janeiro, RJ,Brazil.
authors.forename: Glaucia
authors.initials: G
authors.lastname: Vilar-Pereira
authors.affiliation: Laboratório de Investigação Cardiovascular,Instituto Oswaldo Cruz,Fiocruz, Rio de Janeiro, RJ,Brazil.
authors.forename: Raquel Rangel
authors.initials: RR
authors.lastname: Silvares
authors.affiliation: Laboratório de Biologia das Interações,Instituto Oswaldo Cruz,Fiocruz, Rio de Janeiro, RJ,Brazil.
authors.forename: Joseli
authors.initials: J
authors.lastname: Lannes-Vieira
authors.affiliation: Laboratório de Cardiologia Celular e Molecular,Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, UFRJ,Rio de Janeiro,Brazil.
authors.forename: Antônio Carlos
authors.initials: AC
authors.lastname: Campos De Carvalho
mesh_terms: D018674:Adrenergic Antagonists / Q000097:blood*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001145:Arrhythmias, Cardiac / Q000503:physiopathology*; D002598:Chagas Cardiomyopathy / Q000469:parasitology / Q000503:physiopathology; D014355:Chagas Disease / Q000503:physiopathology*; D018678:Cholinergic Agents / Q000097:blood*; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D054969:Primary Dysautonomias / Q000503:physiopathology*; D043585:Receptor, Muscarinic M2 / Q000378:metabolism; D018342:Receptors, Adrenergic, beta-1 / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000502:physiology*
chemical_list: D018674:Adrenergic Antagonists; D000913:Antibodies, Protozoan; D018678:Cholinergic Agents; D043585:Receptor, Muscarinic M2; D018342:Receptors, Adrenergic, beta-1