title: The effect of poly(trimethylene carbonate) molecular weight on macrophage behavior and enzyme adsorption and conformation.
abstract: Poly(trimethylene carbonate) (PTMC) with molecular weights greater than 100 kg/mol is known to degrade readily in vivo while PTMC of less than 70 kg/mol is resistant to degradation. The reason for the molecular weight dependent degradation rate of PTMC is unclear, and may be due to differences in macrophage behavior or enzyme adsorption or activity. Macrophage number and production of reactive oxygen species (ROS) and esterase were measured when cultured on 60 and 100 kg/mol PTMC. Cholesterol esterase and lipase were adsorbed to 60 and 100 kg/mol PTMC and mass and viscoelastic properties of the adsorbed enzyme layers were measured. No significant differences were observed in macrophage number or production of degradative species. Significant differences were measured in mass, shear modulus and viscosity of the adsorbed cholesterol esterase layer, suggesting that the cholesterol esterase is adsorbing in a different conformation on the 60 and 100 kg/mol PTMC. Despite similar bulk moduli, the surface modulus of 60 kg/mol PTMC was significantly lower than 100 kg/mol. It is proposed that the difference in surface stiffness and polymer chain flexibility affect the arrangement of water bound to and freed from the polymer chains during adsorption, thus affecting enzymatic adsorption, conformation, and activity.
authors.affiliation: Department of Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada.
authors.forename: Moira C
authors.initials: MC
authors.lastname: Vyner
authors.affiliation: Department of Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada.
authors.forename: Anne
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada. Electronic address: brian.amsden@chee.queensu.ca.
authors.forename: Brian G
authors.initials: BG
authors.lastname: Amsden
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000493:pharmacokinetics; D002460:Cell Line; D002478:Cells, Cultured; D004146:Dioxanes / Q000737:chemistry* / Q000493:pharmacokinetics; D008049:Lipase / Q000737:chemistry; D008264:Macrophages / Q000166:cytology / Q000187:drug effects*; D008422:Materials Testing; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry* / Q000493:pharmacokinetics; D011487:Protein Conformation; D017382:Reactive Oxygen Species / Q000378:metabolism; D002787:Sterol Esterase / Q000737:chemistry / Q000493:pharmacokinetics; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D004146:Dioxanes; D011108:Polymers; D017382:Reactive Oxygen Species; C059299:polytrimethylene carbonate; D002787:Sterol Esterase; D008049:Lipase
keywords: Enzymatic degradation; Macrophage; Poly(trimethylene carbonate); Polymer chain mobility; Protein adsorption