title: Carboplatin loaded protein nanoparticles exhibit improve anti-proliferative activity in retinoblastoma cells.
abstract: Retinoblastoma, a common neoplasm of eye in children accounts about 9-10% of all paediatric cancer. Carboplatin (carbo) is preferred chemotherapeutic regimen. In this study the prospective of carboplatin loaded apotranferrin (Apo-nano-carbo) and lactoferrin (Lacto-nano-carbo) nanoparticles have been demonstrated for the treatment of retinoblastoma. Apo-nano-carbo and Lacto-nano-carbo were prepared by sol-oil method (as a patented formula) with size of 82-92 nm and 68-81 nm, hydrodynamic size were 142±15 nm and 263±20 nm, encapsulation efficiency were 50%±2.3 and 52%±3.9 respectively. Results of pH dependent-drug release and receptor-blocking assay showed that nanoparticles may deliver drug through receptor mediated endocytosis. The carboplatin loaded nanoparticles shows greater intracellular uptake, sustained retention and thus, high anti-proliferative activity (Apo-nano-carbo IC50=4.31 μg ml(-1), Lacto-nano-carbo IC50=4.16 μg ml(-1), Sol-carbo IC50=13.498 μg ml(-1)) into the retinoblastoma cells compared to their soluble counterpart.
authors.affiliation: Department Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, India.
authors.forename: Farhan
authors.initials: F
authors.lastname: Ahmed
authors.affiliation: L.V. Prasad Eye Institute, Hyderabad, India.
authors.forename: Mohammad Javed
authors.initials: MJ
authors.lastname: Ali
authors.affiliation: Department Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, India. Electronic address: akksl@uohyd.ernet.in.
authors.forename: Anand K
authors.initials: AK
authors.lastname: Kondapi
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D016190:Carboplatin / Q000008:administration & dosage*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D053938:DNA Fragmentation / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry; D065546:Drug Liberation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011506:Proteins / Q000737:chemistry*; D012175:Retinoblastoma / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D011506:Proteins; D016190:Carboplatin; D053148:Caspase 3
keywords: Carboplatin; Protein nanoparticle; Retinoblastoma