title: Mechanism of curcumin resistance to human cytomegalovirus in HELF cells.
abstract: BACKGROUND
  We have previously shown that curcumin exhibited an outstanding anti-HCMV effect in vitro and in vivo. However, the underlying mechanism for the anti-HCMV effect remains unclear.
  
  
  METHODS
  Levels of IL-6 and TNF-α cytokine secretions in HELF cells were determined by enzyme-linked immunosorbent assay (ELISA); cell cycles were assessed by flow cytometry; ie and ul83 gene expressions were evaluated using reverse transcriptase real-time quantitative PCR; HCMV IE and UL83 antigen expressions were studied using immunofluorescence staining assay and western blot.
  
  
  RESULTS
  Curcumin reduced HCMV immediate early antigen (IEA) and UL83A expressions and IL-6, and TNF-α secretions and recovered cell proliferation to normal level in HCMV infected HELF cells.
  
  
  CONCLUSIONS
  Curcumin anti-HCMV effect may possibly be that curcumin concurrently alters host cell microenviroment and inhibits the HCMV antigen expressions. These findings may provide a basic understanding of the curcumin anti-HCMV effect and a novel strategy for further development of curcumin anti-HCMV treatment.
authors.forename: Yali
authors.initials: Y
authors.lastname: Lv
authors.forename: Zhuoling
authors.initials: Z
authors.lastname: An
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Zihui
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of pharmaceutical affairs, Beijing Chao-Yang Hospital, Capital Medical University, No, 8 GongrenTiyuchangNanlu, Chaoyang District, 100020 Beijing, P, R, China. hongllh@126.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Liu
mesh_terms: D002460:Cell Line; D003474:Curcumin / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D003587:Cytomegalovirus / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D016207:Cytokines; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D003474:Curcumin
references.citation: PLoS Pathog. 2012 Sep;8(9):e1002905
references.pmid: 23028311
references.citation: J Gen Virol. 2011 Dec;92(Pt 12):2757-2769
references.pmid: 21832009
references.citation: J Virol. 2010 Aug;84(15):7803-14
references.pmid: 20504932
references.citation: J Virol. 1997 Feb;71(2):1629-34
references.pmid: 8995690
references.citation: Oncol Lett. 2013 Jul;6(1):81-85
references.pmid: 23946781
references.citation: PLoS One. 2013;8(2):e56795
references.pmid: 23451089
references.citation: Clin Transplant. 2001 Oct;15(5):297-308
references.pmid: 11678955
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: J Virol. 1998 May;72(5):3729-41
references.pmid: 9557655
references.citation: J Med Virol. 2001 May;64(1):29-34
references.pmid: 11285565
references.citation: J Virol. 2001 Apr;75(8):3613-25
references.pmid: 11264351
references.citation: J Virol. 2011 Dec;85(23):12750-8
references.pmid: 21937636
references.citation: Virol J. 2011 Jul 29;8:374
references.pmid: 21798067
references.citation: Ontogenez. 2001 Jan-Feb;32(1):29-34
references.pmid: 11234389
references.citation: PLoS Pathog. 2009 May;5(5):e1000427
references.pmid: 19436702
references.citation: Biosci Biotechnol Biochem. 2009 Jun;73(6):1268-73
references.pmid: 19502735
references.citation: Curr Top Microbiol Immunol. 2008;325:1-19
references.pmid: 18637497
references.citation: Virus Res. 2011 May;157(2):151-60
references.pmid: 21056604
references.citation: J Antimicrob Chemother. 2002 Feb;49(2):243-53
references.pmid: 11815564
references.citation: Viruses. 2013 Sep 19;5(9):2272-81
references.pmid: 24056673
references.citation: PLoS Pathog. 2012;8(8):e1002901
references.pmid: 22952450
references.citation: Pharmacol Ther. 2003 Jun;98(3):269-97
references.pmid: 12782241
references.citation: Transpl Infect Dis. 2009 Jun;11(3):195-202
references.pmid: 19228345
references.citation: J Biol Chem. 2012 Sep 28;287(40):33198-214
references.pmid: 22847000
references.citation: Viral Immunol. 2012 Apr;25(2):98-105
references.pmid: 22486303
references.citation: Epidemiol Infect. 2009 Jan;137(1):58-65
references.pmid: 18413004
references.citation: Pharmacol Ther. 2011 Sep;131(3):309-29
references.pmid: 21570424
references.citation: J Virol. 2000 Aug;74(15):7108-18
references.pmid: 10888651