title: Prevalence of human herpesvirus 6A and 6B during pregnancy.
abstract: The aim of the present study was to assess the frequency of human herpesvirus 6A (HHV-6A) and human herpesvirus 6B (HHV-6B) infection during pregnancy. 100-100 blood samples were collected from pregnant and non-pregnant women, then nucleic acid was isolated from both plasma and leukocytes fraction. Nested and real-time PCR were used to detect and differentiate HHV-6A and HHV-6B DNA and to determine viral loads. Reverse transcription PCR (RT-PCR) for HHV-6 U79/80 mRNA was performed in order to reveal active HHV-6 replication.HHV-6A and HHV-6B active infections were not detected in blood samples neither from pregnant nor from non-pregnant women. Frequency of HHV-6B and HHV-6A latency did not show difference between the studied groups (15% vs. 16%). HHV-6B latency was dominant in both studied groups (14/15 and 15/16). Beside these results, in leukocyte samples of one pregnant and three non-pregnant women high HHV-6A viral loads (1.28 × 10⁵ - 5.07 × 10⁵ GEq / 1.5 × 10⁶ leukocytes) were detected, and viral DNA was also found in plasma samples. Although RT-PCR did not confirm virus replication, but chromosomal integration was also not proved unequivocally, the number of 0.08-0.33 HHV-6 copy / 1 leukocyte refers more to postnatal infection.
authors.affiliation: University of Debrecen Department of Medical Microbiology Debrecen Hungary.
authors.forename: Beáta
authors.initials: B
authors.lastname: Mészáros
authors.affiliation: University of Debrecen Department of Obstetrics and Gynaecology Debrecen Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Sápy
authors.affiliation: University of Debrecen Department of Medical Microbiology Debrecen Hungary.
authors.forename: Lajos
authors.initials: L
authors.lastname: Gergely
authors.affiliation: University of Debrecen Department of Medical Microbiology Debrecen Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Csoma
mesh_terms: D000293:Adolescent; D000328:Adult; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015654:Herpesvirus 6, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D011247:Pregnancy; D011248:Pregnancy Complications / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D019349:Roseolovirus Infections / Q000453:epidemiology* / Q000821:virology; D019562:Viral Load; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: HHV-6A; HHV-6B; PCR; blood; healthy women; pregnancy