title: Novel insights into the metabolic and biochemical underpinnings assisting dry-season survival in female malaria mosquitoes of the Anopheles gambiae complex.
abstract: The mechanisms by which Anopheles gambiae mosquitoes survive the desiccating conditions of the dry season in Africa and are able to readily transmit malaria soon after the rains start remain largely unknown. The desiccation tolerance and resistance of female An. gambiae M and S reared in contrasting environmental conditions reflecting the onset of dry season ("ods") and the rainy season ("rs") was determined by monitoring their survival and body water loss in response to low relative humidity. Furthermore, we investigated the degree to which the physiology of 1-h and 24-h-old females is altered at "ods" by examining and comparing their quantitative metabotypes and proteotypes with conspecifics exposed to "rs" conditions. Results showed that distinct biochemical rearrangements occurred soon after emergence in female mosquitoes that enhance survival and limit water loss under dry conditions. In particular, three amino acids (phenylalanine, tyrosine, and valine) playing a pivotal role in cuticle permeability decreased significantly from the 1-h to 24-h-old females, regardless of the experimental conditions. However, these amino acids were present in higher amounts in 1-h-old female An. gambiae M reared under "ods" whereas no such seasonal difference was reported in S ones. Together with the 1.28- to 2.84-fold increased expression of cuticular proteins 70 and 117, our data suggests that cuticle composition, rigidity and permeability were adjusted at "ods". Increased expression of enzymes involved in glycogenolytic and proteolytic processes were found in both forms at "ods". Moreover, 1-h-old S forms were characterised by elevated amounts of glycogen phosphorylase, isocitrate dehydrogenase, and citrate synthase, suggesting an increase of energetic demand in these females at "ods".
authors.affiliation: Université de Rennes 1, UMR CNRS 6553 Ecobio, Campus de Beaulieu, 263 Avenue du Général Leclerc, CS 74205, 35042 Rennes Cedex, France; Institut de Recherche pour le Développement (IRD), UMR IRD 224-CNRS 5290-Université de Montpellier 1, Université de Montpellier 2 MIVEGEC, 911 Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France. Electronic address: kevin.hidalgo@univ-rennes1.fr.
authors.forename: K
authors.initials: K
authors.lastname: Hidalgo
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR IRD 224-CNRS 5290-Université de Montpellier 1, Université de Montpellier 2 MIVEGEC, 911 Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France; Institut de Recherche en Sciences de la Santé (IRSS), Direction Régionale de l'Ouest (DRO), 399 Avenue de la Liberté, 01 BP 545, Bobo-Dioulasso, Burkina Faso.
authors.forename: K
authors.initials: K
authors.lastname: Mouline
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR IRD 224-CNRS 5290-Université de Montpellier 1, Université de Montpellier 2 MIVEGEC, 911 Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France; Institut de Recherche en Sciences de la Santé (IRSS), Direction Régionale de l'Ouest (DRO), 399 Avenue de la Liberté, 01 BP 545, Bobo-Dioulasso, Burkina Faso.
authors.forename: W
authors.initials: W
authors.lastname: Mamai
authors.affiliation: Université Claude Bernard Lyon 1, UMR CNRS 5023 LEHNA, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
authors.forename: N
authors.initials: N
authors.lastname: Foucreau
authors.affiliation: Institut de Recherche en Sciences de la Santé (IRSS), Direction Régionale de l'Ouest (DRO), 399 Avenue de la Liberté, 01 BP 545, Bobo-Dioulasso, Burkina Faso.
authors.forename: K R
authors.initials: KR
authors.lastname: Dabiré
authors.affiliation: Université de Rennes 1, UMR INRA IGEPP, Campus de Beaulieu, 263 Avenue du Général Leclerc, CS 74205, 35042 Rennes Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Bouchereau
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR IRD 224-CNRS 5290-Université de Montpellier 1, Université de Montpellier 2 MIVEGEC, 911 Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France.
authors.forename: F
authors.initials: F
authors.lastname: Simard
authors.affiliation: Université de Rennes 1, UMR CNRS 6553 Ecobio, Campus de Beaulieu, 263 Avenue du Général Leclerc, CS 74205, 35042 Rennes Cedex, France.
authors.forename: D
authors.initials: D
authors.lastname: Renault
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000852:Anopheles / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D001834:Body Water / Q000502:physiology; D002241:Carbohydrates / Q000032:analysis; D003681:Dehydration / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D040901:Proteomics; D012621:Seasons
chemical_list: D000596:Amino Acids; D002241:Carbohydrates
keywords: Body water; Desiccation; Metabolic fingerprint; Proteomics; RR-2 cuticular protein; Tricarboxylic acid cycle enzyme