title: Crystallization and preliminary X-ray diffraction analysis of YidC, a membrane-protein chaperone and insertase from Bacillus halodurans.
abstract: YidC, a member of the YidC/Oxa1/Alb3 family, inserts proteins into the membrane and facilitates membrane-protein folding in bacteria. YidC plays key roles in both Sec-mediated integration and Sec-independent insertion of membrane proteins. Here, Bacillus halodurans YidC2, which has five transmembrane helices conserved among the other family members, was identified as a target protein for structure determination by a fluorescent size-exclusion chromatography analysis. The protein was overexpressed, purified and crystallized in the lipidic cubic phase. The crystals diffracted X-rays to 2.4 Å resolution and belonged to space group P21, with unit-cell parameters a = 43.9, b = 60.6, c = 58.9 Å, β = 100.3°. The experimental phases were determined by the multiwavelength anomalous diffraction method using a mercury-derivatized crystal.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kumazaki
authors.affiliation: Department of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Tsukazaki
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Nishizawa
authors.affiliation: Department of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USA.
authors.forename: Hideaki E
authors.initials: HE
authors.lastname: Kato
authors.affiliation: Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Nakada-Nakura
authors.affiliation: SR Life Science Instrumentation Unit, RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Hirata
authors.affiliation: Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mori
authors.affiliation: Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suga
authors.affiliation: Global Research Cluster, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Ishitani
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Nureki
mesh_terms: D001407:Bacillus / Q000737:chemistry* / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry*; D002850:Chromatography, Gel; D018360:Crystallography, X-Ray; D004798:Enzymes / Q000737:chemistry*; D018832:Molecular Chaperones / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D018832:Molecular Chaperones
keywords: Bacillus halodurans; YidC; lipidic cubic phase; membrane-protein insertase
references.citation: Annu Rev Biophys. 2009;38:29-51
references.pmid: 19086821
references.citation: Nature. 2011 May 11;474(7350):235-8
references.pmid: 21562494
references.citation: Annu Rev Biophys. 2012;41:21-40
references.pmid: 22224601
references.citation: Mol Cell. 2009 May 15;34(3):344-53
references.pmid: 19450532
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: J Biol Chem. 2008 Apr 4;283(14):9350-8
references.pmid: 18234665
references.citation: Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
references.pmid: 20383002
references.citation: FEBS Lett. 2010 Jun 18;584(12):2539-47
references.pmid: 20394746
references.citation: Methods Enzymol. 1997;276:472-494
references.pmid: 27799110
references.citation: J Biol Chem. 2003 May 2;278(18):15622-32
references.pmid: 12586834
references.citation: J Biol Chem. 2008 Feb 22;283(8):5208-16
references.pmid: 18093969
references.citation: Acta Crystallogr A. 2008 Jan;64(Pt 1):112-22
references.pmid: 18156677
references.citation: Biol Chem. 2012 Nov;393(11):1279-90
references.pmid: 23111630
references.citation: EMBO Rep. 2001 Jun;2(6):524-9
references.pmid: 11415986
references.citation: Mol Microbiol. 2014 Jan;91(2):408-21
references.pmid: 24261830
references.citation: Biochemistry. 2006 Nov 7;45(44):13401-8
references.pmid: 17073462
references.citation: Nature. 2000 Aug 10;406(6796):637-41
references.pmid: 10949305
references.citation: Biol Chem. 2011 Jan;392(1-2):13-9
references.pmid: 21194367
references.citation: Biochim Biophys Acta. 2014 Aug;1843(8):1489-96
references.pmid: 24418623
references.citation: J Struct Biol. 2010 Jul;171(1):82-7
references.pmid: 20152903
references.citation: Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):352-67
references.pmid: 22505256
references.citation: Bioinformatics. 2004 Sep 1;20(13):2138-9
references.pmid: 15044227
references.citation: Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):30-42
references.pmid: 15299723
references.citation: Structure. 2006 Apr;14(4):673-81
references.pmid: 16615909
references.citation: J Biol Chem. 2013 Jun 7;288(23):16295-16307
references.pmid: 23609445
references.citation: Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):849-61
references.pmid: 10089316