title: Molecular cloning and bioinformatics analysis of a new plasma membrane Na⁺/H⁺ antiporter gene from the halophyte Kosteletzkya virginica.
abstract: A new plasma membrane Na(+)/H(+) antiporter gene (named as KvSOS1) was cloned from the halophyte Kosteletzkya virginica by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technology, which is a homologue of SOS1 (salt overly sensitive 1). The full-length cDNA is 3850 bp and contains an open reading frame (ORF) encoding a protein of 1147 amino acids with a molecular weight of 127.56 kDa and a theoretical pI of 6.18. Bioinformatics analysis indicated that the deduced protein appears to be a transmembrane protein with 12 transmembrane domains at the N-terminal region and a long hydrophilic tail in cytoplasm at its C-terminal region and shares 72-82% identity at the peptide level with other plant plasma membrane Na(+)/H(+) antiporters.
authors.affiliation: Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, China ; Yantai Academy of China Agricultural University, Yantai 264670, China ; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, China ; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Tang
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Taian 271018, China.
authors.forename: Chuyang
authors.initials: C
authors.lastname: Shao
authors.affiliation: Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, China ; Institute for Life Sciences, Qingdao University of Science & Technology (QUST), Qingdao 266042, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Shao
authors.affiliation: Yantai Academy of China Agricultural University, Yantai 264670, China.
authors.forename: Honglei
authors.initials: H
authors.lastname: Wang
mesh_terms: D003001:Cloning, Molecular; D019295:Computational Biology / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D055051:Salt-Tolerant Plants / Q000235:genetics* / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D017923:Sodium-Hydrogen Exchangers
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