title: Identification of prohibitin as an antigen in Behcet's disease.
abstract: OBJECTIVE
  This study is intended to screen potential antigen for Behcet's disease (BD) by using human microvascular endothelial cells (HUVEC).
  
  
  METHODS
  Following cell-based indirect immunofluorescence assay with sera from BD patients, proteins extracted from HUVEC were separated and detected by Western blotting. Then the target protein was identified by LC-MALDI-TOF/TOF, the recombinant target protein was expressed, purified and then used as coating antigen to test the prevalence of autoantibodies in patient's sera.
  
  
  RESULTS
  The Western blotting result showed that some patients' sera could react with a protein band with about 30 kDa of molecular weight, which was further identified as prohibitin by mass spectrometry. The prevalence of serum antibodies against recombinant human prohibitin was detected in 16 of 58 BD patients (28%) but none in healthy controls.
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Xun
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Yan
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Weikang
authors.initials: W
authors.lastname: Yang
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Shi
authors.affiliation: ImmunoHunt Corporation, 139 Fengtai Rd, Beijing 100071, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Chen
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China. Electronic address: hongwudu@ustb.edu.cn.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Du
mesh_terms: D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000276:immunology; D001528:Behcet Syndrome / Q000097:blood / Q000276:immunology*; D015415:Biomarkers / Q000097:blood*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D000091402:Prohibitins; D012097:Repressor Proteins / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D015415:Biomarkers; D000091402:Prohibitins; D012097:Repressor Proteins
keywords: Autoantigen; Autoimmune; Behcet’s disease; Prohibitin