title: The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans.
abstract: BACKGROUND
  Streptococcus mutans is known to be a primary etiological factor of dental caries, a widespread and growing disease in Polish children. Recognition of novel features determining the pathogenicity of this pathogen may contribute to understanding the mechanisms of bacterial infections.The goal of the study was to determine the activity of prephenate dehydrogenase (PHD) and to illuminate the role of the enzyme in S. mutans pathogenicity. The strains were biotyped based on STREPTOtest 24 biochemical identification tests and the usefulness of biotyping in the determination of S. mutans pathogenicity determinants was examined.
  
  
  RESULTS
  Out of ninety strains isolated from children with deciduous teeth fifty three were classified as S. mutans species. PDH activity was higher (21.69 U/mg on average) in the experimental group compared to the control group (5.74 U/mg on average) (P <0.001). Moreover, it was demonstrated that biotype I, established basing on the biochemical characterization of the strain, was predominant (58.5%) in oral cavity streptococcosis. Its dominance was determined by higher PDH activity compared to biotypes II and III (P = 0.0019).
  
  
  CONCLUSIONS
  The usefulness of biotyping in the determination of Streptococcus mutans pathogenicity determinants was demonstrated. The obtained results allow for better differentiation of S. mutans species and thus may contribute to recognition of pathogenic bacteria transmission mechanisms and facilitate treatment.
authors.forename: Wirginia
authors.initials: W
authors.lastname: Krzyściak
authors.forename: Katarzyna K
authors.initials: KK
authors.lastname: Pluskwa
authors.forename: Jakub
authors.initials: J
authors.lastname: Piątkowski
authors.forename: Paweł
authors.initials: P
authors.lastname: Krzyściak
authors.forename: Anna
authors.initials: A
authors.lastname: Jurczak
authors.forename: Dorota
authors.initials: D
authors.lastname: Kościelniak
authors.affiliation: Genetics Laboratory, Department of Endocrinology, Jagiellonian University, Medical College, Kopernika 17, 31-501 Krakow, Poland. anna.skalniak@uj.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Skalniak
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D009055:Mouth / Q000382:microbiology*; D011303:Prephenate Dehydrogenase / Q000032:analysis*; D013295:Streptococcus mutans / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification / Q000472:pathogenicity; D037521:Virulence Factors / Q000032:analysis*
chemical_list: D037521:Virulence Factors; D011303:Prephenate Dehydrogenase
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