title: 6,6'-bieckol isolated from Ecklonia cava protects oxidative stress through inhibiting expression of ROS and proinflammatory enzymes in high-glucose-induced human umbilical vein endothelial cells.
abstract: Hyperglycemia-induced oxidative stress accelerates endothelial cell dysfunctions, which cause various complications of diabetes. The protective effects of 6,6'-bieckol (BEK), one of phlorotannin compound purified from Ecklonia cava against high-glucose-induced oxidative stress was investigated using human umbilical vein endothelial cells (HUVECs), which is susceptible to oxidative stress. High glucose (30 mM) treatment induced HUVECs' cell death, but BEK, at concentration 10 or 50 μg/ml, significantly inhibited the high-glucose-induced cytotoxicity. Furthermore, treatment with BEK dose-dependently decreased thiobarbituric acid reactive substances (TBARS), intracellular reactive oxygen species (ROS) generation, and nitric oxide level increased by high glucose. In addition, high glucose levels induced the overexpressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor-kappa B (NF-κB) proteins in HUVECs, but BEK treatment reduced the overexpressions of these proteins. These findings indicate that BEK is a potential therapeutic agent that will prevent diabetic endothelial dysfunction and related complications.
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan, 617-736, Republic of Korea.
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Park
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Heo
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.forename: Si-Heung
authors.initials: SH
authors.lastname: Sung
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Seung-Hong
authors.initials: SH
authors.lastname: Lee
mesh_terms: D051546:Cyclooxygenase 2 / Q000378:metabolism; D004147:Dioxins / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000633:toxicity*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D000459:Phaeophyceae / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D004147:Dioxins; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; C062360:6,6'-bieckol; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D005947:Glucose