title: Carbon partitioning in green algae (chlorophyta) and the enolase enzyme.
abstract: The exact mechanisms underlying the distribution of fixed carbon within photoautotrophic cells, also referred to as carbon partitioning, and the subcellular localization of many enzymes involved in carbon metabolism are still unknown. In contrast to the majority of investigated green algae, higher plants have multiple isoforms of the glycolytic enolase enzyme, which are differentially regulated in higher plants. Here we report on the number of gene copies coding for the enolase in several genomes of species spanning the major classes of green algae. Our genomic analysis of several green algae revealed the presence of only one gene coding for a glycolytic enolase [EC 4.2.1.11]. Our predicted cytosolic localization would require export of organic carbon from the plastid to provide substrate for the enolase and subsequent re-import of organic carbon back into the plastids. Further, our comparative sequence study of the enolase and its 3D-structure prediction may suggest that the N-terminal extension found in green algal enolases could be involved in regulation of the enolase activity. In summary, we propose that the enolase represents one of the crucial regulatory bottlenecks in carbon partitioning in green algae.
authors.affiliation: Department of Biology, Brooklyn College of the City University of New York, 2900 Bedford Avenue 200NE, Brooklyn, NY 11210, USA. jpolle@brooklyn.cuny.edu.
authors.forename: Jürgen E W
authors.initials: JE
authors.lastname: Polle
authors.affiliation: Department of Biology, Brooklyn College of the City University of New York, 2900 Bedford Avenue 200NE, Brooklyn, NY 11210, USA. pneofotis@gc.cuny.edu.
authors.forename: Peter
authors.initials: P
authors.lastname: Neofotis
authors.affiliation: Department of Biology, Brooklyn College of the City University of New York, 2900 Bedford Avenue 200NE, Brooklyn, NY 11210, USA. gnauhandy@gmail.com.
authors.forename: Andy
authors.initials: A
authors.lastname: Huang
authors.affiliation: Department of Biology, Brooklyn College of the City University of New York, 2900 Bedford Avenue 200NE, Brooklyn, NY 11210, USA. williamchang3389@gmail.com.
authors.forename: William
authors.initials: W
authors.lastname: Chang
authors.affiliation: Department of Biology, Brooklyn College of the City University of New York, 2900 Bedford Avenue 200NE, Brooklyn, NY 11210, USA. kiran_sury92@yahoo.com.
authors.forename: Kiran
authors.initials: K
authors.lastname: Sury
authors.affiliation: Department of Biology, Brooklyn College of the City University of New York, 2900 Bedford Avenue 200NE, Brooklyn, NY 11210, USA. EWiech@gc.cuny.edu.
authors.forename: Eliza M
authors.initials: EM
authors.lastname: Wiech
references.citation: Biochim Biophys Acta. 2010 Apr;1803(4):520-5
references.pmid: 20144902
references.citation: J Biol Chem. 2011 Jan 7;286(1):429-40
references.pmid: 20961853
references.citation: Plant Cell. 1991 Jul;3(7):719-35
references.pmid: 1841726
references.citation: Plant Cell. 2011 May;23(5):1904-19
references.pmid: 21540433
references.citation: Mol Biosyst. 2009 Dec;5(12):1889-903
references.pmid: 19763335
references.citation: Planta. 1986 Jan;167(1):81-6
references.pmid: 24241735
references.citation: Cell Mol Life Sci. 2001 Jun;58(7):902-20
references.pmid: 11497239
references.citation: Plant J. 2013 Nov;76(3):481-93
references.pmid: 23952686
references.citation: Plant Cell. 2010 Sep;22(9):2943-55
references.pmid: 20852019
references.citation: Biotechnol Bioeng. 2013 Sep;110(9):2317-22
references.pmid: 23733523
references.citation: Bioresour Technol. 2011 Jan;102(1):111-7
references.pmid: 20619638
references.citation: Plant Sci. 2012 Apr;185-186:9-22
references.pmid: 22325862
references.citation: Exp Eye Res. 1992 Feb;54(2):253-60
references.pmid: 1559553
references.citation: Genome Biol. 2012 May 25;13(5):R39
references.pmid: 22630137
references.citation: J Appl Phycol. 2012 Apr;24(2):253-266
references.pmid: 22427720
references.citation: Proc Natl Acad Sci U S A. 2007 May 1;104(18):7705-10
references.pmid: 17460045
references.citation: Biochem J. 2013 Apr 15;451(2):145-54
references.pmid: 23535167
references.citation: Plant Physiol. 2008 Sep;148(1):156-75
references.pmid: 18633119
references.citation: Mol Biochem Parasitol. 2014 Feb;193(2):75-81
references.pmid: 24602601
references.citation: New Phytol. 2009 Nov;184(3):607-618
references.pmid: 19694966
references.citation: J Mol Biol. 2004 Aug 20;341(4):1015-21
references.pmid: 15289101
references.citation: Plant Cell. 2014 Jun 3;26(6):2310-2350
references.pmid: 24894045
references.citation: Metabolites. 2014 Apr 11;4(2):184-217
references.pmid: 24957022
references.citation: Science. 2007 Oct 12;318(5848):245-50
references.pmid: 17932292
references.citation: Nature. 2012 Aug 16;488(7411):329-35
references.pmid: 22895338
references.citation: J Biol Chem. 1998 Jun 5;273(23):14503-15
references.pmid: 9603964
references.citation: Science. 2012 Jun 29;336(6089):1663-7
references.pmid: 22745419
references.citation: Curr Opin Chem Biol. 2013 Jun;17(3):453-6
references.pmid: 23542013
references.citation: Parasitol Res. 2007 Nov;101(6):1511-6
references.pmid: 17653767
references.citation: Bioresour Technol. 2011 Jan;102(1):123-9
references.pmid: 20594832
references.citation: Mol Biol Cell. 2005 Oct;16(10):4509-18
references.pmid: 16030251
references.citation: Annu Rev Plant Biol. 2013;64:583-607
references.pmid: 23451781
references.citation: Curr Genet. 2011 Jun;57(3):151-68
references.pmid: 21533645
references.citation: Mol Biol Evol. 2012 Dec;29(12):3625-39
references.pmid: 22826458
references.citation: J Cell Biol. 2005 Jul 4;170(1):103-13
references.pmid: 15998802
references.citation: Curr Opin Chem Biol. 2013 Jun;17(3):444-52
references.pmid: 23587864
references.citation: J Exp Bot. 2012 Mar;63(6):2293-308
references.pmid: 22371075
references.citation: Science. 2010 Jul 9;329(5988):223-6
references.pmid: 20616280
references.citation: Biochim Biophys Acta. 1991 Mar 4;1073(2):410-5
references.pmid: 1826218
references.citation: J Mol Biol. 2004 Oct 29;343(4):997-1005
references.pmid: 15476816
references.citation: New Phytol. 2010 Feb;185(3):649-62
references.pmid: 20002588
references.citation: Mol Biochem Parasitol. 2012 Aug;184(2):122-5
references.pmid: 22569588
references.citation: J Biol Chem. 2000 Feb 25;275(8):5958-65
references.pmid: 10681589
references.citation: Gene. 2004 Oct 13;340(2):227-35
references.pmid: 15475163
references.citation: Eukaryot Cell. 2013 Jun;12(6):776-93
references.pmid: 23543671
references.citation: BMC Genomics. 2011 Dec 22;12 Suppl 4:S5
references.pmid: 22369158
references.citation: Science. 2009 Apr 10;324(5924):268-72
references.pmid: 19359590
references.citation: FEBS Lett. 2009 Mar 18;583(6):983-91
references.pmid: 19223001
references.citation: Mol Cell Biol. 1999 Apr;19(4):2880-6
references.pmid: 10082554
references.citation: Biochemistry. 2014 Apr 29;53(16):2722-31
references.pmid: 24697546
references.citation: Plant Cell. 2010 Aug;22(8):2594-617
references.pmid: 20798327
references.citation: Mol Biosyst. 2010 Jun;6(6):1018-31
references.pmid: 20358043
references.citation: J Biomed Biotechnol. 2012;2012:156795
references.pmid: 23118496
references.citation: Biochemistry. 2011 Nov 22;50(46):9950-62
references.pmid: 21999478
references.citation: J Proteomics. 2013 Dec 6;94:207-18
references.pmid: 24120529
references.citation: Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
references.pmid: 15034147
references.citation: Microbes Infect. 2004 May;6(6):604-8
references.pmid: 15158195
references.citation: Plant Physiol Biochem. 2010 Dec;48(12):961-5
references.pmid: 20951055
references.citation: Curr Top Microbiol Immunol. 2013;358:155-213
references.pmid: 22143554