title: Molecular identification of Aedes triseriatus and Aedes hendersoni by a novel duplex polymerase chain reaction assay.
abstract: Aedes triseriatus is the principal vector of La Crosse virus (LACv), which is the most common cause of pediatric arboviral encephalitis in North America. Here we report a novel species-specific polymerase chain reaction (PCR) assay that differentially identifies Ae. triseriatus and Ae. hendersoni. Because these 2 sibling species differ in their abilities to transmit LACv, accurate identification is critical for surveillance, research, and control programs. This duplex assay can detect the presence of both species in a single PCR reaction and is therefore simpler and faster than previously reported methods.
authors.forename: Robert
authors.initials: R
authors.lastname: Wilson
authors.forename: Robin
authors.initials: R
authors.lastname: Harrison
authors.forename: Michael
authors.initials: M
authors.lastname: Riles
authors.forename: Gideon
authors.initials: G
authors.lastname: Wasserberg
authors.forename: Brian D
authors.initials: BD
authors.lastname: Byrd
mesh_terms: D000330:Aedes / Q000145:classification* / Q000235:genetics / Q000821:virology; D000818:Animals; D004275:DNA, Ribosomal / Q000235:genetics; D007303:Insect Vectors / Q000145:classification* / Q000235:genetics / Q000821:virology; D018062:La Crosse virus / Q000502:physiology; D044969:Ochlerotatus / Q000145:classification* / Q000235:genetics / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D014481:United States
chemical_list: D004275:DNA, Ribosomal