title: Intratumorally implanted mesenchymal stromal cells potentiate peripheral immunotherapy against malignant rat gliomas.
abstract: Bone marrow-derived mesenchymal stromal cells (MSCs) target glioma extensions and micro-satellites efficiently when implanted intratumorally. Here, we report that intratumoral implantation of MSCs and peripheral immunotherapy with interferon-gamma (IFNγ) producing tumor cells improve the survival of glioma-bearing rats (54% cure rate) compared to MSC alone (0% cure rate) or immunotherapy alone (21% cure rate) by enforcing an intratumoral CD8(+) T cell response. Further analysis revealed that the MSCs up-regulate MHC classes I and II in response to IFNγ treatment in vitro and secrete low amounts of immunosuppressive molecules prostaglandin E2 and interleukin-10.
authors.affiliation: Bengzon Group, Lund Stem Cell Center, Division of Neurosurgery, Department of Clinical Sciences, Lund University, BMC B10, SE-221 84 Lund, Sweden.
authors.forename: Salina
authors.initials: S
authors.lastname: Ströjby
authors.affiliation: Bengzon Group, Lund Stem Cell Center, Division of Neurosurgery, Department of Clinical Sciences, Lund University, BMC B10, SE-221 84 Lund, Sweden. Electronic address: sofia.eberstal@med.lu.se.
authors.forename: Sofia
authors.initials: S
authors.lastname: Eberstål
authors.affiliation: Bengzon Group, Lund Stem Cell Center, Division of Neurosurgery, Department of Clinical Sciences, Lund University, BMC B10, SE-221 84 Lund, Sweden.
authors.forename: Andreas
authors.initials: A
authors.lastname: Svensson
authors.affiliation: Glioma Immunotherapy Group, Division of Neurosurgery, Department of Clinical Sciences, Lund University, Barngatan 2B, SE-221 85 Lund, Sweden.
authors.forename: Sara
authors.initials: S
authors.lastname: Fritzell
authors.affiliation: Bengzon Group, Lund Stem Cell Center, Division of Neurosurgery, Department of Clinical Sciences, Lund University, BMC B10, SE-221 84 Lund, Sweden; Translational Cancer Research, Lund University, Medicon Village 404:C3, Scheelevägen 2, SE-223 81 Lund, Sweden.
authors.forename: Daniel
authors.initials: D
authors.lastname: Bexell
authors.affiliation: Glioma Immunotherapy Group, Division of Neurosurgery, Department of Clinical Sciences, Lund University, Barngatan 2B, SE-221 85 Lund, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Siesjö
authors.affiliation: Glioma Immunotherapy Group, Division of Neurosurgery, Department of Clinical Sciences, Lund University, Barngatan 2B, SE-221 85 Lund, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Darabi
authors.affiliation: Bengzon Group, Lund Stem Cell Center, Division of Neurosurgery, Department of Clinical Sciences, Lund University, BMC B10, SE-221 84 Lund, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Bengzon
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000276:immunology / Q000628:therapy*; D045744:Cell Line, Tumor; D005910:Glioma / Q000276:immunology / Q000628:therapy*; D007167:Immunotherapy / Q000379:methods*; D015552:Injections, Intralesional; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D013601:T-Lymphocytes / Q000276:immunology
keywords: Glioma; Immunotherapy; Interferon-gamma; Mesenchymal stromal cells; Rat