title: The RNase R from Campylobacter jejuni has unique features and is involved in the first steps of infection.
abstract: Bacterial pathogens must adapt/respond rapidly to changing environmental conditions. Ribonucleases (RNases) can be crucial factors contributing to the fast adaptation of RNA levels to different environmental demands. It has been demonstrated that the exoribonuclease polynucleotide phosphorylase (PNPase) facilitates survival of Campylobacter jejuni in low temperatures and favors swimming, chick colonization, and cell adhesion/invasion. However, little is known about the mechanism of action of other ribonucleases in this microorganism. Members of the RNB family of enzymes have been shown to be involved in virulence of several pathogens. We have searched C. jejuni genome for homologues and found one candidate that displayed properties more similar to RNase R (Cj-RNR). We show here that Cj-RNR is important for the first steps of infection, the adhesion and invasion of C. jejuni to eukaryotic cells. Moreover, Cj-RNR proved to be active in a wide range of conditions. The results obtained lead us to conclude that Cj-RNR has an important role in the biology of this foodborne pathogen.
authors.affiliation: From the LUNAM Université, Oniris, University of Nantes, 44200 Nantes, France, the UMR1014 Sécurité des Aliments et Microbiologie, INRA, 44322 Nantes, France, and nabila.haddad@oniris-nantes.fr.
authors.forename: Nabila
authors.initials: N
authors.lastname: Haddad
authors.affiliation: the Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av da República, 2780-157 Oeiras, Portugal.
authors.forename: Rute G
authors.initials: RG
authors.lastname: Matos
authors.affiliation: the Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av da República, 2780-157 Oeiras, Portugal.
authors.forename: Teresa
authors.initials: T
authors.lastname: Pinto
authors.affiliation: From the LUNAM Université, Oniris, University of Nantes, 44200 Nantes, France, the UMR1014 Sécurité des Aliments et Microbiologie, INRA, 44322 Nantes, France, and.
authors.forename: Pauline
authors.initials: P
authors.lastname: Rannou
authors.affiliation: From the LUNAM Université, Oniris, University of Nantes, 44200 Nantes, France, the UMR1014 Sécurité des Aliments et Microbiologie, INRA, 44322 Nantes, France, and.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Cappelier
authors.affiliation: From the LUNAM Université, Oniris, University of Nantes, 44200 Nantes, France, the UMR1014 Sécurité des Aliments et Microbiologie, INRA, 44322 Nantes, France, and.
authors.forename: Hervé
authors.initials: H
authors.lastname: Prévost
authors.affiliation: the Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av da República, 2780-157 Oeiras, Portugal cecilia@itqb.unl.pt.
authors.forename: Cecília M
authors.initials: CM
authors.lastname: Arraiano
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002169:Campylobacter Infections / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D005095:Exoribonucleases; C080868:ribonuclease R
keywords: Adhesion; Campylobacter; Foodborne Pathogen; Invasion; RNA Catalysis; RNA Metabolism; Ribonuclease
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