title: Up-regulation of HDAC4 is associated with Schwann cell proliferation after sciatic nerve crush.
abstract: Histone deacetylase 4 (HDAC4), a member of the class IIa HDACs subfamily, has emerged as a critical regulator of cell growth, differentiation, and migration in various cell types. It was reported that HDAC4 stimulated colon cell proliferation via repression of p21. Also, HDAC4 contributes to platelet-derived growth factor-BB-induced proliferation and migration of vascular smooth muscle cells. Furthermore, HDAC4 may play an important role in the regulation of neuronal differentiation and survival. However, the role of HDAC4 in the process of peripheral nervous system regeneration after injury remains virtually unknown. Herein, we investigated the spatiotemporal expression of HDAC4 in a rat sciatic nerve crush model. We found that sciatic nerve crush induced up-regulated expression of HDAC4 in Schwann cells. Moreover, the expression of the proliferation marker Ki-67 exhibited a similar tendency with that of HDAC4. In cell cultures, we observed increased expression of HDAC4 during the process of TNF-α-induced Schwann cell proliferation, whereas the protein level of p21 was down-regulated. Interference of HDAC4 led to enhanced expression of p21 and impaired proliferation of Schwan cells. Taken together, our findings implicated that HDAC4 was up-regulated in the sciatic nerve after crush, which was associated with proliferation of Schwann cells.
authors.affiliation: Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Nie
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Zhu
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Liu
authors.forename: Xingxing
authors.initials: X
authors.lastname: Mao
authors.forename: Shixian
authors.initials: S
authors.lastname: Yan
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Ni
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D006655:Histone Deacetylases / Q000378:metabolism*; D008297:Male; D009409:Nerve Crush / Q000379:methods; D009416:Nerve Regeneration / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism*; D012584:Sciatic Nerve / Q000293:injuries*; D015854:Up-Regulation
chemical_list: C559944:HDAC4 protein, rat; D006655:Histone Deacetylases
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