title: Association of eight EST-derived SNPs with carcass and meat quality traits in pigs.
abstract: The identification of genetic markers associated with important economic traits is fundamental to improving the productivity and quality of livestock. In this investigation, we searched for 177 expressed sequence tags (ESTs) putatively involved in meat quality from the available pig EST database, and detected eight single nucleotide polymorphisms (SNPs) in eight ESTs. We investigated the associations of these SNPs with 18 carcass and meat quality traits in a Landrace × Lantang F2 resource population (n = 257). Association analysis revealed that seven SNPs (except E42) were associated with some of the carcass- and meat quality-related traits. Particularly, significant associations of three SNPs (E53, E82, and E36) with backfat thickness traits were observed. Further, the genetic effects of E53 on four live backfat thickness traits were validated in an independent population (n = 221). More investigations about E53 sequence characteristics were performed, i.e., radiation hybrid (RH) mapping, 3'-RACE, and screening analysis of the positive BAC clones. Our research identified the genetic effects of eight EST-derived SNPs on carcass and meat quality traits, and suggested that E53 may be a useful marker for live backfat thickness traits in pig breeding programs.
authors.affiliation: College of Animal Science, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou, Guangdong, 510642, People's Republic of China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Tong
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yiren
authors.initials: Y
authors.lastname: Jiao
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Hongquyen
authors.initials: H
authors.lastname: Dang
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Jiang
authors.forename: Junli
authors.initials: J
authors.lastname: Duan
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Li
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
mesh_terms: D050154:Adiposity / Q000235:genetics; D000818:Animals; D020224:Expressed Sequence Tags; D005260:Female; D005838:Genotype; D008297:Male; D008460:Meat / Q000032:analysis*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D021101:Radiation Hybrid Mapping; D034421:Sus scrofa / Q000235:genetics*
references.citation: Proc Natl Acad Sci U S A. 1973 Dec;70(12):3321-3
references.pmid: 4519626
references.citation: Meat Sci. 2002 Feb;60(2):113-24
references.pmid: 22063234
references.citation: J Biochem. 2011 Jul;150(1):23-37
references.pmid: 21610046
references.citation: Mol Biol Cell. 2002 Dec;13(12):4484-96
references.pmid: 12475967
references.citation: Nucleic Acids Res. 2013 Jan;41(Database issue):D871-9
references.pmid: 23180796
references.citation: J Anim Breed Genet. 2007 Nov;124 Suppl 1:2-11
references.pmid: 17988245
references.citation: Nature. 2012 Nov 15;491(7424):393-8
references.pmid: 23151582
references.citation: Biochem J. 2012 Feb 1;441(3):763-87
references.pmid: 22248338
references.citation: Science. 1991 Jun 21;252(5013):1651-6
references.pmid: 2047873
references.citation: Int J Evol Biol. 2012;2012:596274
references.pmid: 22811948
references.citation: Anim Sci J. 2012 Jan;83(1):14-22
references.pmid: 22250734
references.citation: Anim Biotechnol. 2012;23(1):43-63
references.pmid: 22292700
references.citation: Trends Cell Biol. 2009 Aug;19(8):385-94
references.pmid: 19648010
references.citation: Mamm Genome. 2001 Aug;12(8):630-6
references.pmid: 11471058
references.citation: Anim Genet. 2004 Apr;35(2):123-5
references.pmid: 15025572
references.citation: Anim Genet. 1998 Dec;29(6):415-24
references.pmid: 9883502
references.citation: Meat Sci. 2005 Jul;70(3):423-34
references.pmid: 22063742
references.citation: BMC Proc. 2009 Jul 16;3 Suppl 4:S14
references.pmid: 19615114
references.citation: Nat Genet. 1999 Dec;23(4):452-6
references.pmid: 10581034
references.citation: Anim Genet. 2002 Feb;33(1):3-18
references.pmid: 11849132
references.citation: PLoS One. 2011 Mar 22;6(3):e18067
references.pmid: 21445367
references.citation: Anim Genet. 2000 Feb;31(1):8-12
references.pmid: 10690355
references.citation: Yi Chuan Xue Bao. 2004 Dec;31(12):1361-8
references.pmid: 15633641
references.citation: Bioinformatics. 2000 Jun;16(6):558-9
references.pmid: 10980153
references.citation: Plant Physiol. 2003 Feb;131(2):419-29
references.pmid: 12586867
references.citation: Nat Genet. 1993 Aug;4(4):332-3
references.pmid: 8401577
references.citation: Meat Sci. 2005 Jan;69(1):143-9
references.pmid: 22062650
references.citation: Cytogenet Cell Genet. 1998;82(3-4):182-8
references.pmid: 9858812
references.citation: J Biol Chem. 2007 Jun 29;282(26):19122-32
references.pmid: 17420258
references.citation: Gigascience. 2012 Nov 15;1(1):16
references.pmid: 23587058
references.citation: Obesity (Silver Spring). 2009 Apr;17(4):632-9
references.pmid: 19148129
references.citation: Nucleic Acids Res. 2002 Oct 1;30(19):4103-17
references.pmid: 12364589
references.citation: J Biol Chem. 2001 Aug 3;276(31):28824-8
references.pmid: 11375985
references.citation: Am J Physiol Regul Integr Comp Physiol. 2004 Sep;287(3):R502-16
references.pmid: 15308499
references.citation: Nat Rev Genet. 2002 May;3(5):391-7
references.pmid: 11988764
references.citation: Meat Sci. 2007 Jun;76(2):359-65
references.pmid: 22064307
references.citation: Biochem Genet. 2009 Apr;47(3-4):315-21
references.pmid: 19189208
references.citation: Trends Plant Sci. 2003 Jul;8(7):321-9
references.pmid: 12878016