title: Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.
abstract: In this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutral red retention and 7-ethoxyresorufin-O-deethylase assays. The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems. However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe. Thus, the absolute pollution mass transfer of Yangtze River may be detrimental to the environmental quality of estuary and East China Sea. Effect-directed analysis was applied to identify substances causing high dioxin-like activities. To identify unknown substances contributing to dioxin-like potencies of whole extracts, we fractionated crude extracts by open column chromatography. Non-polar paraffinic components (F1), weakly and moderately polar components (F2), and highly polar substances (F3) were separated from each crude extract of sediments. F2 showed the highest dioxin-like activities. Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts. Further studies should be conducted to identify unknown pollutants.
authors.affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China; Department of Ecosystem Analysis, Institute for Environmental Research (Biology V), Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Ecosystem Analysis, Institute for Environmental Research (Biology V), Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany.
authors.forename: Tilman
authors.initials: T
authors.lastname: Floehr
authors.affiliation: Department of Ecosystem Analysis, Institute for Environmental Research (Biology V), Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Ecosystem Analysis, Institute for Environmental Research (Biology V), Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Ecosystem Analysis, Institute for Environmental Research (Biology V), Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Eichbaum
authors.affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China; Department of Ecosystem Analysis, Institute for Environmental Research (Biology V), Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany; College of Resources and Environmental Science, Chongqing University, Chongqing, China; School of Environment, Nanjing University, Nanjing, China.
authors.forename: Henner
authors.initials: H
authors.lastname: Hollert
authors.affiliation: Key Laboratory of Yangtze Water environment, Ministry of Education, Tongji University, Shanghai, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D002681:China; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis / Q000378:metabolism; D054750:Ecotoxicology; D004790:Enzyme Induction / Q000187:drug effects; D063366:Estuaries; D019015:Geologic Sediments / Q000737:chemistry*; D017686:Oncorhynchus mykiss / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism / Q000633:toxicity*; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D045483:Rivers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D018336:Receptors, Aryl Hydrocarbon; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1
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