title: Proteomic white adipose tissue analysis of obese mice fed with a high-fat diet and treated with oral angiotensin-(1-7).
abstract: Angiotensin-(1-7) has been described as a new potential therapeutic tool for the treatment and prevention of metabolic disorders by regulating several pathways in visceral white adipose tissue (vWAT). The aim of this study was to access the proteins differentially regulated by Ang-(1-7) using proteomic analysis of visceral adipose tissue. Male mice were divided into three groups and fed for 60 days, with each group receiving one of the following diets: standard diet+HPβCD (ST), high fat diet+HPβCD (HFD) and high fat diet+Ang-(1-7)/HPβCD (HFD+Ang-(1-7)). Body weight, fat weight and food intake were measured. At the end of treatment, Ang-(1-7) induced a decrease in body and fat weight. Differential proteomic analysis using two-dimensional electrophoresis (2-DE) combined with mass spectrometry were performed. Results of protein mapping of mesenteric adipose tissue using 2-DE revealed the presence of about 450 spots in each gel (n=3/treatment) with great reproducibility (>70%). Image analysis and further statistical analysis allowed the detection and identification of eight proteins whose expression was modulated in response to HFD when compared to ST. Among these, two proteins showed a sensitive response to Ang-(1-7) treatment (eno1 and aldehyde dehydrogenase). In addition, three proteins were expressed statistically different between HFD+Ang-(1-7) and HFD groups, and four proteins were modulated compared to standard diet. In conclusion, comparative proteomic analysis of a mice model of diet-induced obesity allowed us to outline possible pathways involved in the response to Ang-(1-7), suggesting that Ang-(1-7) may be a useful tool for the treatment of metabolic disorders.
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (UNIMONTES), Montes Claros, Minas Gerais, Brazil; Nursing Department, Universidade Estadual de Montes Claros (UNIMONTES), Montes Claros, Minas Gerais, Brazil.
authors.forename: João Marcus Oliveira
authors.initials: JM
authors.lastname: Andrade
authors.affiliation: Pharmacology Department, Biological Sciences Institute (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Fernanda de Oliveira
authors.initials: Fde O
authors.lastname: Lemos
authors.affiliation: Parasitology Department, Biological Sciences Institute (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Simone
authors.initials: S
authors.lastname: da Fonseca Pires
authors.affiliation: Department of Chemistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Ruben Dario Sinisterra
authors.initials: RD
authors.lastname: Millán
authors.affiliation: Department of Chemistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Frederico Barros
authors.initials: FB
authors.lastname: de Sousa
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (UNIMONTES), Montes Claros, Minas Gerais, Brazil.
authors.forename: André Luiz Sena
authors.initials: AL
authors.lastname: Guimarães
authors.affiliation: Touro University Nevada College of Osteopathic Medicine, Las Vegas, NV, United States.
authors.forename: Mahboob
authors.initials: M
authors.lastname: Qureshi
authors.affiliation: Touro University Nevada College of Osteopathic Medicine, Las Vegas, NV, United States.
authors.forename: John David
authors.initials: JD
authors.lastname: Feltenberger
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (UNIMONTES), Montes Claros, Minas Gerais, Brazil.
authors.forename: Alfredo Maurício Batista
authors.initials: AM
authors.lastname: de Paula
authors.affiliation: Physical Training School, Universidade Estadual de Montes Claros (UNIMONTES), Montes Claros, Minas Gerais, Brazil.
authors.forename: Jaime Tolentino Miranda
authors.initials: JT
authors.lastname: Neto
authors.affiliation: Pharmacology Department, Biological Sciences Institute (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Miriam Teresa Paz
authors.initials: MT
authors.lastname: Lopes
authors.affiliation: Parasitology Department, Biological Sciences Institute (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Hélida Monteiro de
authors.initials: HM
authors.lastname: Andrade
authors.affiliation: National Institute of Science and Technology (INCT-NanoBiofar), Physiology Department, Biological Sciences Institute (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Robson Augusto Souza
authors.initials: RA
authors.lastname: Santos
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros (UNIMONTES), Montes Claros, Minas Gerais, Brazil; Pharmacology Department, Biological Sciences Institute (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil. Electronic address: sergiosousas@hotmail.com.
authors.forename: Sérgio Henrique Sousa
authors.initials: SH
authors.lastname: Santos
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects* / Q000378:metabolism*; D000284:Administration, Oral; D000803:Angiotensin I / Q000008:administration & dosage* / Q000494:pharmacology*; D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008815:Mice, Inbred Strains; D009765:Obesity / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D015430:Weight Gain / Q000187:drug effects
chemical_list: D010446:Peptide Fragments; D020543:Proteome; D000803:Angiotensin I; C118790:angiotensin I (1-7)
keywords: Ang-(1–7); Metabolism; Obesity; Proteomic; Renin–angiotensin system