title: Parathyroid hormone (1-34) prevents cartilage degradation and preserves subchondral bone micro-architecture in guinea pigs with spontaneous osteoarthritis.
abstract: OBJECTIVE
  To assess whether parathyroid hormone (PTH) (1-34) could improve the micro-structure of subchondral bone, and retard cartilage degradation in a naturally occurring Osteoarthritis (OA) model.
  
  
  METHODS
  Forty-eight 1-month-old guinea pigs were divided into two groups: 32 were treated by normal saline (NS) and sacrificed at 1, 3, 6 and 9 months of age; the other 16 received PTH (1-34) from 3 months, and were sacrificed at 6 and 9 months. Masson staining and the Osteoarthritis Research Society International (OARSI) grade scores were used to assess cartilage degradation. Immunohistochemistry analyses of type-II collagen, matrix metalloproteinases-13 (MMP-13) and sclerostin (SOST) in the cartilage, osteoprotegerin (OPG) and receptor activator of nuclear factor-kB ligand (RANKL) and PTH receptor (PTH1R) in the cartilage and subchondral bone were performed. Subchondral bone micro-architecture was assessed by micro-computed tomography (micro-CT).
  
  
  RESULTS
  Histological analyses revealed OA occurred at 3 months of age and was more severe with increasing age, and PTH (1-34) reduced the OARSI scores at 6 and 9 months of age. Micro-CT analysis indicated that PTH (1-34) treatment increased the bone volume ratio and bone mineral density (BMD), while retarding the subchondral trabecular bone micro-architectural changes from rod-like to plate-like. Immunohistochemical staining demonstrated that PTH (1-34) treatment increased type-II collagen expression and decreased SOST and MMP-13 expression in the cartilage, while elevating the PTH1R, OPG/RANKL expression ratio in the cartilage and subchondral trabecular bone when compared with the control groups.
  
  
  CONCLUSIONS
  PTH (1-34) can prevent cartilage damage progression and retard the deterioration of subchondral trabecular bone in guinea pigs.
authors.affiliation: Department of Orthopedic Surgery, Hebei Medical University, Shijiazhuang, China. Electronic address: tsyjydoctor55@163.com.
authors.forename: J-y
authors.initials: JY
authors.lastname: Yan
authors.affiliation: Medical Research Center, Hebei United University, Tangshan, China. Electronic address: tfm9911316@163.com.
authors.forename: F-m
authors.initials: FM
authors.lastname: Tian
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Hebei United University, Tangshan, China. Electronic address: wangwenya-3@126.com.
authors.forename: W-Y
authors.initials: WY
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, The Affiliated Hospital of Hebei United University, Tangshan, China. Electronic address: chengying2010@yeah.net.
authors.forename: Y
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Orthopedic Surgery, The Affiliated Hospital of Hebei United University, Tangshan, China. Electronic address: songhuiping@163.com.
authors.forename: H-P
authors.initials: HP
authors.lastname: Song
authors.affiliation: Department of Orthopedic Surgery, Hebei Medical University, Shijiazhuang, China. Electronic address: yingze1953@163.com.
authors.forename: Y-Z
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, Hebei Medical University, Shijiazhuang, China; Department of Orthopedic Surgery, The Affiliated Hospital of Hebei United University, Tangshan, China. Electronic address: zhliu130@sohu.com.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D010003:Osteoarthritis / Q000000981:diagnostic imaging / Q000473:pathology / Q000517:prevention & control*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D010281:Parathyroid Hormone
keywords: Cartilage; Micro-CT; Osteoarthritis; PTH (1-34); Subchondral bone