title: Aging related changes in mixed basal saliva concentration of sodium, potassium and chloride in healthy non medicated humans.
abstract: It is well known that the salivary flow is reduced by aging but ionic composition changes associated to aging have been less evaluated. To measure salivary and plasmatic [Na(+)], [K(+)] and [Cl(-)] and to correlate with age in healthy, non-medicated subjects of any gender, 165 healthy participating subjects (over 15 years old) were asked to give sample of 5 mL mix basal saliva in a plastic vial without any stimulation technique, additionally, 5 mL of venous blood was collected. Samples [Na(+)] and [K(+)] were measured by flame photometry (Corning™ M-405) and [Cl(-)] by voltametric chlorometry (Corning™ M-920). Ionic concentrations were expressed as (X±DE; meq.L⁻¹). All three ionic concentrations progressively increased with age, with the lineal regression equation being: [Na(+)] mEq=17.76 + 0.26(Age); r=+0.42; F=31.5; P=0.00001; [K(+)] mEq=13.2+0.15(Age); r=+0.32; F=16.5; P=0.00001; [Cl(-)] mEq=9.05+0.18(Age); r=+0.35; F=7.8; P=0.0071. Age induced changes in salivary ionic concentrations were not associated to blood ionic changes. However, saliva and blood [Na(+)] and [K(+)] were correlated (r=+0.25; F=4.49; P=0.04 and r=+0.30; F=6.98; P=0.01, respectively). Significant association was found among salivary ions: [Na(+)] mEq=9.14+0.99[K(+)] (r=+0.79; F=95.2; P=0.000001); [Cl(-)] mEq=0.95+0.56[Na(+)] (r=0.79; F=106.6; P=0.000001) and [Cl(-)] mEq=3.45+0.69[K(+)] (r=0.73; F=72.5; P=0.000001). These results confirm and measure the impact of aging over the mixed and resting salivary secretion process and suggest that local changes are not related to blood ionic composition.
authors.forename: Rui
authors.initials: R
authors.lastname: De Oliveira
authors.forename: Eunice
authors.initials: E
authors.lastname: Navas
authors.forename: Carolina
authors.initials: C
authors.lastname: Duran
authors.forename: Maria
authors.initials: M
authors.lastname: Pinto
authors.forename: Jose
authors.initials: J
authors.lastname: Gutierrez
authors.affiliation: Lab. Neurofisiologia, Escuela de Ciencias Biomedicas y Tecnologicas, Facultad de Ciencias de la Salud. Universidad de Carabobo, Venezuela. aeblen@uc.edu.ve.
authors.forename: Antonio
authors.initials: A
authors.lastname: Eblen-Zajjur
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood / Q000378:metabolism*; D002712:Chlorides / Q000032:analysis* / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D064368:Healthy Volunteers; D006706:Homeostasis; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D011188:Potassium / Q000032:analysis* / Q000097:blood; D012463:Saliva / Q000737:chemistry*; D012964:Sodium / Q000032:analysis* / Q000097:blood; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D002712:Chlorides; D012964:Sodium; D011188:Potassium