title: Long-term time-lapse multimodal intravital imaging of regeneration and bone-marrow-derived cell dynamics in skin.
abstract: A major challenge for translating cell-based therapies is understanding the dynamics of cells and cell populations in complexin vivoenvironments. Intravital microscopy has shown great promise for directly visualizing cell behaviorin vivo. However, current methods are limited to relatively short imaging times (hours), by ways to track cell and cell population dynamics over extended time-lapse periods (days to weeks to months), and by relatively few imaging contrast mechanisms that persist over extended investigations. We present technology to visualize and quantify complex, multifaceted dynamic changes in natural deformable skin over long time periods using novel multimodal imaging and a non-rigid image registration method. These are demonstrated in green fluorescent protein (GFP) bone marrow (BM) transplanted mice to study dynamic skin regeneration. This technology provides a novel perspective for studying dynamic biological processes and will enable future studies of stem, immune, and tumor cell biologyin vivo.
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA ; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Benedikt W
authors.initials: BW
authors.lastname: Graf
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Chaney
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Marina
authors.initials: M
authors.lastname: Marjanovic
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Adie
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA ; Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: De Lisio
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Valero
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA ; Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Marni D
authors.initials: MD
authors.lastname: Boppart
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA ; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA ; Departments of Bioengineering and Internal Medicine, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Boppart
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