title: Semiquantitative fluorescence method for bioconjugation analysis.
abstract: Quantum dots (QDs) have been used as fluorescent probes in biological and medical fields such as bioimaging, bioanalytical, and immunofluorescence assays. For these applications, it is important to characterize the QD-protein bioconjugates. This chapter provides details on a versatile method to confirm quantum dot-protein conjugation including the required materials and instrumentation in order to perform the step-by-step semiquantitative analysis of the bioconjugation efficiency by using fluorescence plate readings. Although the protocols to confirm the QD-protein attachment shown here were developed for CdTe QDs coated with specific ligands and proteins, the principles are the same for other QDs-protein bioconjugates.
authors.affiliation: Research Group on Biomedical Nanotechnology, Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Aluízio G
authors.initials: AG
authors.lastname: Brasil
authors.forename: Kilmara H G
authors.initials: KH
authors.lastname: Carvalho
authors.forename: Elisa S
authors.initials: ES
authors.lastname: Leite
authors.forename: Adriana
authors.initials: A
authors.lastname: Fontes
authors.forename: Beate Saegesser
authors.initials: BS
authors.lastname: Santos
mesh_terms: D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry; D002417:Cattle; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D007136:Immunoglobulins / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013691:Tellurium / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D055767:Immobilized Proteins; D007136:Immunoglobulins; D014867:Water; D012710:Serum Albumin, Bovine; D013691:Tellurium; C028337:cadmium telluride