title: Lipid cell biology. Polyunsaturated phospholipids facilitate membrane deformation and fission by endocytic proteins.
abstract: Phospholipids (PLs) with polyunsaturated acyl chains are extremely abundant in a few specialized cellular organelles such as synaptic vesicles and photoreceptor discs, but their effect on membrane properties is poorly understood. Here, we found that polyunsaturated PLs increased the ability of dynamin and endophilin to deform and vesiculate synthetic membranes. When cells incorporated polyunsaturated fatty acids into PLs, the plasma membrane became more amenable to deformation by a pulling force and the rate of endocytosis was accelerated, in particular, under conditions in which cholesterol was limiting. Molecular dynamics simulations and biochemical measurements indicated that polyunsaturated PLs adapted their conformation to membrane curvature. Thus, by reducing the energetic cost of membrane bending and fission, polyunsaturated PLs may help to support rapid endocytosis.
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, Université Nice Sophia Antipolis and CNRS, 06560 Valbonne, France. Unité Mixte de Recherche 144, Institut Curie and CNRS, F-75248 Paris, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Pinot
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, Université Nice Sophia Antipolis and CNRS, 06560 Valbonne, France.
authors.forename: Stefano
authors.initials: S
authors.lastname: Vanni
authors.affiliation: Centre Commun de Microscopie Appliquée, Université Nice Sophia Antipolis, Parc Valrose, 06000 Nice, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Pagnotta
authors.affiliation: Centre Commun de Microscopie Appliquée, Université Nice Sophia Antipolis, Parc Valrose, 06000 Nice, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Lacas-Gervais
authors.affiliation: Signalisation et Transports Ioniques Membranaires, Université de Poitiers and CNRS, Poitiers, France.
authors.forename: Laurie-Anne
authors.initials: LA
authors.lastname: Payet
authors.affiliation: Signalisation et Transports Ioniques Membranaires, Université de Poitiers and CNRS, Poitiers, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Ferreira
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, Université Nice Sophia Antipolis and CNRS, 06560 Valbonne, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Gautier
authors.affiliation: Unité Mixte de Recherche 144, Institut Curie and CNRS, F-75248 Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Goud
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, Université Nice Sophia Antipolis and CNRS, 06560 Valbonne, France. antonny@ipmc.cnrs.fr.
authors.forename: Bruno
authors.initials: B
authors.lastname: Antonny
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, Université Nice Sophia Antipolis and CNRS, 06560 Valbonne, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Barelli
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology*; D034281:Dynamins / Q000737:chemistry / Q000378:metabolism; D004705:Endocytosis; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D056004:Molecular Dynamics Simulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D005231:Fatty Acids, Unsaturated; D008567:Membranes, Artificial; C504641:endophilin A1 protein, mouse; D034281:Dynamins