title: Accumulation of cytolytic CD8+ T cells in B16-melanoma and proliferation of mature T cells in TIS21-knockout mice after T cell receptor stimulation.
abstract: In vivo and in vitro effects of TIS21 gene on the mature T cell activation and antitumor activities were explored by employing MO5 melanoma orthograft and splenocytes isolated from the TIS21-knockout (KO)(2) mice. Proliferation and survival of mature T cells were significantly increased in the KO than the wild type (WT3)e cells, indicating that TIS21 inhibits the rate of mature T cell proliferation and its survival. In MO5 melanoma orthograft model, the KO mice recruited much more CD8(+) T cells into the tumors at around day 14 after tumor cell injection along with reduced tumor volumes compared with the WT. The increased frequency of granzyme B+ CD8+ T cells in splenocytes of the KO mice compared with the WT may account for antitumor-immunity of TIS21 gene in the melanoma orthograft. In contrast, reduced frequencies of CD107a+ CD8+ T cells in the splenocytes of KO mice may affect the loss of CD8+ T cell infiltration in the orthograft at around day 19. These results indicate that TIS21 exhibits antiproliferative and proapoptotic effects in mature T cells, and differentially affects the frequencies of granzyme B+ CD8+ T-cells and CD107a+ CD8+ T-cells, thus transiently regulating in vivo anti-tumor immunity.
authors.affiliation: Department of Biochemistry and Molecular Biology, Ajou University School of Medicine, 164, World cul-ro, Yeongtong-gu, Suwon, Gyeonggi-do 443-380, Republic of Korea.
authors.forename: Min Sook
authors.initials: MS
authors.lastname: Ryu
authors.affiliation: Department of Microbiology, Ajou University School of Medicine, 164, World cul-ro, Yeongtong-gu, Suwon, Gyeonggi-do 443-380, Republic of Korea; Department of Biomedical Sciences, The Graduate School, Ajou University, Republic of Korea.
authors.forename: Min-Yeong
authors.initials: MY
authors.lastname: Woo
authors.affiliation: Department of Microbiology, Kwandong University College of Medicine, Gangneung, Gangwon-do 210-701, Republic of Korea.
authors.forename: Daeho
authors.initials: D
authors.lastname: Kwon
authors.affiliation: Department of Biochemistry and Molecular Biology, Ajou University School of Medicine, 164, World cul-ro, Yeongtong-gu, Suwon, Gyeonggi-do 443-380, Republic of Korea.
authors.forename: Allen E
authors.initials: AE
authors.lastname: Hong
authors.affiliation: Department of Pathology, Chung-Ang University College of Medicine, Dongjak-gu, Seoul 156-756, Republic of Korea.
authors.forename: Kye Yong
authors.initials: KY
authors.lastname: Song
authors.affiliation: Department of Microbiology, Ajou University School of Medicine, 164, World cul-ro, Yeongtong-gu, Suwon, Gyeonggi-do 443-380, Republic of Korea.
authors.forename: Sun
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Biochemistry and Molecular Biology, Ajou University School of Medicine, 164, World cul-ro, Yeongtong-gu, Suwon, Gyeonggi-do 443-380, Republic of Korea.
authors.forename: In Kyoung
authors.initials: IK
authors.lastname: Lim
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D053804:Granzymes / Q000235:genetics / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000502:physiology*; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation; D008297:Male; D008546:Melanoma, Experimental / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000502:physiology*
chemical_list: C489435:Btg2 protein, mouse; D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D025521:Tumor Suppressor Proteins; D053804:Granzymes
keywords: Anti-CD3/anti-CD28 stimulation; CD107a(+) CD8(+) T-cell frequency; MO5-melanoma orthograft; Mature T-cells