title: In vitro anti-quorum sensing activity of phytol.
abstract: Anti-quorum sensing activity of the diterpene phytol was evaluated in vitro for the first time. This compound (at three sub-MIC concentrations - 0.5, 0.25 and 0.125 MIC, respectively) reduced the formation of Pseudomonas aeruginosa PAO1 biofilm in the range of 74.00-84.33% exhibiting higher activity than the both positive controls used, streptomycin and ampicillin. Phytol (0.5 MIC) also effectively reduced P. aeruginosa twitching and flagella motility. Indeed, the bacteria treated were incapable of producing a twitching zone and had almost round, smooth and regular colony edges. Finally, the tested compound (0.5 MIC) exhibited good P. aeruginosa pyocyanin inhibitory activity (51.94%) practically to the same extent as streptomycin (52.09%). According to the experimental data obtained, this phytol property may inspire design of medical foods targeting P. aeruginosa quorum sensing activity.
authors.affiliation: a Department of Life Sciences , Institute for Multidisciplinary Research (IMSI), University of Belgrade , Belgrade , Serbia.
authors.forename: Boris
authors.initials: B
authors.lastname: Pejin
authors.forename: Ana
authors.initials: A
authors.lastname: Ciric
authors.forename: Jasmina
authors.initials: J
authors.lastname: Glamoclija
authors.forename: Milos
authors.initials: M
authors.lastname: Nikolic
authors.forename: Marina
authors.initials: M
authors.lastname: Sokovic
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D015394:Molecular Structure; D010836:Phytol / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D053038:Quorum Sensing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D010836:Phytol
keywords: Pseudomonas aeruginosa PAO1; biofilm; diterpene alcohol; pyocyanin; twitching and flagella motility