title: Cloning and expression pattern of akirin2 gene in broiler.
abstract: Akirin2 is an important nuclear factor which plays functions in innate immune response, myogenesis, muscle development, and carcinogenesis. In this study, akirin2 genes were cloned from 4-day-old Sanhuang and AA(+) broiler, and its expression patterns were analyzed by RT-PCR. The results showed that there were four SNPs in the 5'-terminal region of akirin2 coding sequences. Expression profile analysis showed that the akirin2 transcripts were constitutively expressed in 15 tissues tested, and similar expression patterns were found between the two breeds of broilers. In addition, one of the interesting findings was that the akirin2 gene is highly expressed in blood and lowly expressed in heart, respectively. These data can serve as a foundation for further studying functions of akirin2 gene.
authors.affiliation: College of Life Science and Technology, Harbin Normal University, Harbin, 150025, Heilongjiang Province, People's Republic of China, manchaolai@126.com.
authors.forename: Chaolai
authors.initials: C
authors.lastname: Man
authors.forename: Yang
authors.initials: Y
authors.lastname: Chang
authors.forename: Weitao
authors.initials: W
authors.lastname: Mu
authors.forename: Dongxue
authors.initials: D
authors.lastname: Zhao
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis / Q000235:genetics; D002645:Chickens / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D030161:Avian Proteins; D012097:Repressor Proteins
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