title: Simultaneous determination of 7-O-succinyl macrolactin A and its active major metabolite, macrolactin A in dog plasma using high-performance liquid chromatography with UV detection.
abstract: We developed a method for the simultaneous quantification of 7-O-succinyl macrolactin A and its active metabolite, macrolactin A, in dog plasma. After protein precipitation with acetonitrile including flufenamic acid as an internal standard, 7-O-succinyl macrolactin A, macrolactin A, and flufenamic acid were chromatographed on a reverse-phase C18 analytical column. The mobile phase, consisting of 20 mM acetate buffer and acetonitrile, was eluted using a gradient program at 1 mL/min, and the UV absorbance was measured at 230 nm. The retention times of 7-O-succinyl macrolactin A, flufenamic acid, and macrolactin A were 3.4, 4.8, and 6.9 min, respectively. The coefficient of variation in the assay precision for both substances was less than 6%, and the accuracy ranged from 96 to 105%. This method was used to measure the concentrations of 7-O-succinyl macrolactin A and macrolactin A in dog plasma following an intravenous administration of a single dose (25 mg/kg) of 7-O-succinyl macrolactin A salt.
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, South Korea.
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Kim
authors.forename: Beomsoo
authors.initials: B
authors.lastname: Shin
authors.forename: Kwang-il
authors.initials: KI
authors.lastname: Kwon
authors.forename: Joon Seok
authors.initials: JS
authors.lastname: Bang
authors.forename: Wonku
authors.initials: W
authors.lastname: Kang
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D018942:Macrolides / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: C559723:7-O-succinyl macrolactin A; D018942:Macrolides; C453562:macrolactin A
keywords: 7-O-Succinyl macrolactin A; Dog plasma; High-performance liquid chromatography; Macrolactin A; UV detection