title: The expression of FAS-associated factor 1 and heat shock protein 70 in ovarian cancer.
abstract: OBJECTIVE
  In this study, we evaluated the expression of FAS-associated factor 1 (FAF1) and heat shock protein 70 (HSP70) in normal ovary and ovarian cancer, and also analyzed the correlation between FAF1 and HSP70 in ovarian cancer.
  
  
  METHODS
  The patient group consisted of 29 unrelated Korean women diagnosed as ovarian cancers and control samples were obtained from 7 patients who underwent oophorectomy for benign disease of uterus, and normal ovary was confirmed histologically from biopsy. We examined FAF1 and HSP70 expression by western blot analysis and immunohistochemical staining in normal ovary and ovarian cancer. Furthermore, we examined a correlation between FAF1 and HSP70 in ovarian cancer.
  
  
  RESULTS
  The expression of FAF1 was lower in ovarian cancer than that in normal ovary (P=0.02), and the expression of HSP70 was increased in ovarian cancer in comparison to that in normal ovary (P=0.03). The expression of FAF1 was decreased in advanced stages (stage III or stage IV) as compared with early stages (stage I or stage II) (P=0.01). The expression of HSP70 was not significantly related with ovarian cancer histology (P=0.10), but the expression of HSP70 was most increased with papillary serous carcinomas and undifferentiated ovarian cancer. The expression of FAF1 was inversely correlated with the expression of HSP70 in ovarian cancer (Spearman correlation coefficience=-0.47).
  
  
  CONCLUSIONS
  We concluded that the expression of FAF1 or HSP70 each seems to have a meaning as a biomarker for early detection of ovarian cancer. The expressions of FAF1 and HSP70 seem to be more valuable in predicting ovarian cancer when used together because of their inverse correlation. This is the first study about the expression of FAF1 in ovarian cancer and the correlation between FAF1 and HSP70 expression in ovarian cancer.
authors.affiliation: Mizpark Obstetrics and Gynecology, Incheon, Korea.
authors.forename: Hye-Ju
authors.initials: HJ
authors.lastname: Kang
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University School of Medicine, Seoul, Korea.
authors.forename: Hye-Sung
authors.initials: HS
authors.lastname: Moon
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University School of Medicine, Seoul, Korea.
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Chung
keywords: FAS-associated factor 1; HSP70 heat-shock proteins; Ovarian neoplasms
references.citation: Hum Reprod. 1989 Jan;4(1):1-12
references.pmid: 2651469
references.citation: Science. 1995 Sep 15;269(5230):1585-8
references.pmid: 7545313
references.citation: J Exp Med. 2004 Aug 16;200(4):425-35
references.pmid: 15314073
references.citation: Cell Cycle. 2009 Aug 15;8(16):2528-34
references.pmid: 19597341
references.citation: Expert Rev Mol Diagn. 2010 Sep;10(6):787-98
references.pmid: 20843202
references.citation: Int J Gynecol Pathol. 1983;2(3):275-85
references.pmid: 6196309
references.citation: Pathol Oncol Res. 1998;4(4):316-21
references.pmid: 9887365
references.citation: Oral Oncol. 1998 Mar;34(2):93-8
references.pmid: 9682770
references.citation: Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11894-8
references.pmid: 8524870
references.citation: Cell. 1998 Feb 6;92(3):351-66
references.pmid: 9476895
references.citation: Biochem Biophys Res Commun. 2001 Sep 7;286(5):1027-32
references.pmid: 11527403
references.citation: Int J Oncol. 2003 Oct;23(4):1015-23
references.pmid: 12963981
references.citation: Womens Health (Lond). 2010 Jul;6(4):487-90
references.pmid: 20597610
references.citation: Cancer. 1995 Nov 15;76(10 Suppl):2092-6
references.pmid: 8635006
references.citation: J Korean Med Sci. 1998 Aug;13(4):383-8
references.pmid: 9741542
references.citation: Mol Cancer. 2008 May 19;7:40
references.pmid: 18489772
references.citation: Drug Resist Updat. 2008 Aug-Oct;11(4-5):164-79
references.pmid: 18818117
references.citation: Am J Gastroenterol. 2009 Mar;104(3):630-8
references.pmid: 19174780
references.citation: Eur J Cancer Prev. 1998 Jun;7(3):225-31
references.pmid: 9696931
references.citation: Nature. 1996 Jun 13;381(6583):571-9
references.pmid: 8637592
references.citation: Exp Cell Res. 2003 Feb 1;283(1):1-16
references.pmid: 12565815
references.citation: Am J Respir Cell Mol Biol. 1994 Apr;10(4):453-61
references.pmid: 8136161
references.citation: BMC Cancer. 2005 Jul 09;5:77
references.pmid: 16004614
references.citation: Int J Cancer. 2001 Aug 15;93(4):481-8
references.pmid: 11477551
references.citation: Breast Cancer Res Treat. 1997 Mar;43(1):43-51
references.pmid: 9065598
references.citation: Nat Rev Drug Discov. 2009 Jan;8(1):33-40
references.pmid: 19116625
references.citation: Cancer Detect Prev. 1998;22(6):549-55
references.pmid: 9824379
references.citation: Cancer Res. 2007 Feb 15;67(4):1735-43
references.pmid: 17308115
references.citation: Int J Hyperthermia. 2000 Sep-Oct;16(5):445-8
references.pmid: 11001578
references.citation: J Immunol. 1993 Oct 15;151(8):4286-94
references.pmid: 8409402
references.citation: J Biol Chem. 2004 Jan 23;279(4):2544-9
references.pmid: 14600157
references.citation: Hepatology. 2003 Jan;37(1):198-207
references.pmid: 12500205
references.citation: Nat Med. 2000 Apr;6(4):435-42
references.pmid: 10742151
references.citation: J Biol Chem. 2005 Mar 4;280(9):8125-33
references.pmid: 15596450
references.citation: Gynecol Oncol. 1992 Feb;44(2):147-54
references.pmid: 1312052
references.citation: Oncogene. 2008 Oct 20;27(48):6216-27
references.pmid: 18931689
references.citation: Blood. 2009 Mar 26;113(13):3059-69
references.pmid: 18984860