title: Loading and release of fluorescent dye from layer-by-layer film-coated magnetic particles in response to hydrogen peroxide.
abstract: Polymer-coated magnetic particles (MPs) were prepared to study the binding of fluorescence dye on the surface and its H2O2-induced release. For this goal, multilayer films were prepared by layer-by-layer deposition of shikimic acid-appended poly(allylamine hydrochloride) (SA-PAH) and poly(styrenesulfonate) (PSS) on the surface of MPs. 3-(Dansylamino)phenylboronic acid (DPBA) was loaded on the MPs through boronate ester bonding between SA-PAH and DPBA. DPBA was released from the MPs in response to H2O2 as a result of breakage of the boronate ester bond by an oxidative reaction with H2O2. DPBA release was dependent on the H2O2 concentration. For example, 65% and 93% of the DPBA was released from (SA-PAH/PSS)4SA-PAH film-coated MPs in 30min after the addition of 0.1 and 0.5mM H2O2, respectively. In addition, the multilayer film-coated MPs were further modified by using glucose oxidase (GOx) to develop glucose-induced release systems. GOx-modified MPs released DPBA in response to 0.1mM d-glucose as a result of H2O2 generation through a GOx-catalyzed oxidation reaction of d-glucose. The results suggest a potential use of the multilayer film-coated MPs in the development of H2O2- and/or glucose-sensitive drug delivery systems.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan. Electronic address: satok@m.tohoku.ac.jp.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Sato
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Abe
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Mao
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Anzai
mesh_terms: D001897:Boronic Acids / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D005456:Fluorescent Dyes; D011073:Polyamines; D011137:Polystyrenes; C063994:polyallylamine; C003321:polystyrene sulfonic acid; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; C010686:benzeneboronic acid
keywords: Drug delivery system; Glucose response; Hydrogen peroxide; Layer-by-layer (LbL) film; Phenylboronic acid