title: Crystal structure of TIR domain of TLR6 reveals novel dimeric interface of TIR-TIR interaction for toll-like receptor signaling pathway.
abstract: Toll-like receptors (TLRs) are responsible for recognition of particular pathogens during the innate immune response and cytoplasmic Toll/interleukin-1 receptor (TIR) domain responsible for downstream signaling. TLR6 working with TLR2 can detect bacterial lipoprotein leading signal for nuclear factor-kappaB activation for immune response. To better understand TLR-mediated signaling event in the innate immune system, in this study, we report the first crystal structure of the TIR domain of TLR6 at 2.2Å resolution. Our structure reveals novel homo-dimerization interfaces, which might be a critical for the interaction with TIR-containing adaptor proteins and itself. We also report structural similarities and differences of TLR6 with those of other TIR domains, which may be functionally relevant.
authors.affiliation: School of Biotechnology and Graduate School of Biochemistry, Yeungnam University, 280, Daehak-ro, Gyeongsan-si, Gyeongsangbuk-do 712-749, South Korea.
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Jang
authors.affiliation: School of Biotechnology and Graduate School of Biochemistry, Yeungnam University, 280, Daehak-ro, Gyeongsan-si, Gyeongsangbuk-do 712-749, South Korea. Electronic address: hyunho@ynu.ac.kr.
authors.forename: Hyun Ho
authors.initials: HH
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017472:Receptors, Interleukin-1 / Q000737:chemistry*; D015398:Signal Transduction; D051216:Toll-Like Receptor 6 / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D008562:Membrane Glycoproteins; D017472:Receptors, Interleukin-1; C434984:TIRAP protein, human; C495368:TLR6 protein, human; D051216:Toll-Like Receptor 6
keywords: NF-kappaB; TIR domain; TLR6; crystal structure; innate immunity