title: Development of a new 26plex Y-STRs typing system for forensic application.
abstract: In this study, 26plex Y-STRs typing system, including 17 Y-STRs (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and GATA H4) recommended as YHRD standard loci and nine new highly discriminating Y-STRs (DYS549, DYS643, DYS388, DYS570, DYS533, DYS576, DYS460, DYS481 and DYS449), was established with 5-dye fluorescences labelling. Developmental validation indicated that the 26plex Y-STRs typing system was reproducible, accurate, sensitive and robust. The sensitivity of the system was such that a full profile was obtainable even with 125pg of male DNA. Specificity testing was demonstrated by the lack of cross-reactivity with a variety of commonly encountered animal species and bacteria. Also, the multiplex is suitable for mixture study. An average of above 97% of the minor alleles detected with the male/male mixture with 1:3 and 3:1 ratios, while an average of above 70% of the minor alleles detected with the male/male mixture with 1:19 and 19:1 ratios. Full profiles are consistently detected with 125pg of male DNA, even in the presence of excessive amounts of female DNA. In addition, the whole PCR amplification of the 26 Y-STRs can finish in 1h, making the multiplex system suitable for fast-detection. For the forensic evaluation of the multiplex system, 516 haplotypes were found among 517 unrelated males. HD of the multiplex system was 0.9999925 while DC was 0.9980658, which is suitable for forensic application.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Room 109, 2nd Biology Building, Shanghai 200433, PR China; Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, PR China, Shanghai 200063, PR China.
authors.forename: Suhua
authors.initials: S
authors.lastname: Zhang
authors.affiliation: PEOPLESPOTINC R&D, PR China, Beijing 102206, PR China.
authors.forename: Huaizhou
authors.initials: H
authors.lastname: Tian
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, PR China, Shanghai 200063, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, PR China, Shanghai 200063, PR China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, PR China, Shanghai 200063, PR China; Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, PR China, Shanghai 200063, PR China. Electronic address: lichengtaohla@163.com.
authors.forename: Chengtao
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Room 109, 2nd Biology Building, Shanghai 200433, PR China. Electronic address: chnji@fudan.edu.cn.
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
mesh_terms: D000818:Animals; D016477:Artifacts; D002681:China; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction; D015203:Reproducibility of Results; D013045:Species Specificity
keywords: DNA typing; Forensic science; Validation; Y-STR