title: Dual characterization of biological cells by optofluidic microscope and resistive pulse sensor.
abstract: Label-free detection technique has emerged as a powerful platform for biomedical applications since it can avoid laborious multi-step sample preparation. In this paper, we demonstrate a dual analysis of biological cells using a single microfluidic system combining optofluidic microscopy and resistive pulse sensing. Both red blood cells (RBCs) and circulating tumor cells (CTCs) have been used to validate the concept of dual analysis and also to test the performance of the microfluidic device. The cell characterization by resistive pulse sensing is in good agreement with the analysis by optofluidic microscopy, further verified by the commercial Beckman-Coulter® FC500 flow cytometry. The present system has attractive merits such as simple fabrication, easy integration, high portability, and low cost. This study has great potentials for the development of innovative on-chip flow cytometry with concurrent imaging sensing and resistive sensing.
authors.affiliation: Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore, Singapore; School of Microelectronics and Solid-State Electronics, University of Electronic Science and Technology of China, Chengdu, Sichuan, P. R. China; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Guo
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Xiwei
authors.initials: X
authors.lastname: Huang
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Ai
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Kang
mesh_terms: D004867:Equipment Design; D004912:Erythrocytes; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D056945:Hep G2 Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009360:Neoplastic Cells, Circulating
keywords: Label-free detection; On-chip flow cytometry; Optofluidic microscopy; Resistive pulse sensing