title: Adhesion of suspension cells on a coverslip in serum-free conditions.
abstract: Here, we present a rapid and damage-free fixation protocol for human cells cultured in suspension. Our results demonstrated that serum-free incubation of myeloid suspension cell lines HL-60, U937, and THP-1 for 10 min resulted in cell adhesion to coverslips, allowing simple and efficient fixation for microscopy. The fixed cells exhibited an intact morphology and were suitable for immunostaining. Such simplicity and cost effectiveness have not been achieved by any previously established fixation technique, and our newly developed method provides an additional fixation technique for researchers working with suspension cells.
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Nakayama
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Mihara
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.
authors.forename: Jin
authors.initials: J
authors.lastname: Kawata
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan. Electronic address: hisa@kumamoto-u.ac.jp.
authors.forename: Hisato
authors.initials: H
authors.lastname: Saitoh
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D018914:Cells, Immobilized; D003584:Cytological Techniques / Q000379:methods*; D006801:Humans; D008853:Microscopy; D013194:Staining and Labeling; D013535:Suspensions; D013997:Time Factors; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D013535:Suspensions
keywords: Adhesion; Fixation; HL-60; Immunostaining; Myeloid cell