title: [Optimization of enzymatic extraction of polysaccharide from Dendrobium officinale by box-Behnken design and response surface methodology].
abstract: OBJECTIVE
  To optimize the processing of enzymatic extraction of polysaccharide from Dendrobium officinale.
  
  
  METHODS
  With phenol-sulfuric acid method and the DNS determination polysaccharide, Box-Behnken response surface methodology was used to optimize different enzyme dosage, reaction temperature and reaction time by using Design-Expert 8.05 software for data analysis and processing.
  
  
  RESULTS
  According to Box-Behnken response, the best extraction conditions for the polysaccharide from Dendrobium officinale were as follows: the amount of enzyme complex was 3.5 mg/mL, hydrolysis temperature was 53 degrees C, and reaction time was 70 min. In accordance with the above process, the polysaccharide yield was 16.11%.
  
  
  CONCLUSIONS
  Box-Behnken response surface methodology is used to optimize the enzymatic extraction process for the polysaccharide in this study, which is effective, stable and feasible.
authors.forename: Jian-mei
authors.initials: JM
authors.lastname: Hu
authors.forename: Jing-ling
authors.initials: JL
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Feng
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods; D031667:Dendrobium / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D015233:Models, Statistical; D010636:Phenols / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D013464:Sulfuric Acids / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D004798:Enzymes; D010636:Phenols; D011134:Polysaccharides; D013464:Sulfuric Acids; C033158:sulfuric acid