title: Simultaneous determination of nine lignans from Schisandra chinensis extract using ultra-performance liquid chromatography with tandem mass spectrometry in rat plasma, urine, and gastrointestinal tract samples: application to the pharmacokinetic study of Schisandra chinensis.
abstract: The fruit of Schisandra chinensis is a well-known herbal medicine and dietary supplement due to a variety of biological activities including antihepatotoxic and antihyperlipidemic activities. However, the simultaneous validation methodology and pharmacokinetic investigation of nine lignans of S. chinensis extract in biological samples have not been proved yet. Thus, the present study was undertaken to develop the proper sample preparation method and simultaneous analytical method of schisandrol A, gomisin J, schisandrol B, tigloylgomisin H, angeloylgomisin H, schisandrin A, schisandrin B, gomisin N, and schisandrin C in the hexane-soluble extract of S. chinensis to apply for the pharmacokinetic study in rats. All intra- and interprecisions of nine lignans were below 13.7% and accuracies were 85.1-115% and it is enough to evaluate the pharmacokinetic parameters after both intravenous and oral administration of hexane-soluble extract of S. chinensis to rats.
authors.affiliation: College of Pharmacy and BK21PLUS R-FIND team, Dongguk University-Seoul, Goyang, South Korea.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hee Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Chul Young
authors.initials: CY
authors.lastname: Kim
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Han
authors.forename: Young-Won
authors.initials: YW
authors.lastname: Chin
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Choi
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D041981:Gastrointestinal Tract / Q000378:metabolism*; D017705:Lignans / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D057230:Limit of Detection; D008297:Male; D010936:Plant Extracts / Q000097:blood / Q000737:chemistry* / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D032141:Schisandra / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D017705:Lignans; D010936:Plant Extracts
keywords: Lignans; Liquid chromatography with tandem mass spectrometry; Pharmacokinetics; Rats; Schisandra chinensis