title: [Osteochodral lesion mouse model: an alternative for experimental work].
abstract: OBJECTIVE
  To report a reproducible and inexpensive model of critical osteochondral lesion (LOC) in adult mice for experimental studies
  
  
  METHODS
  An experimental study was conducted on 20 BKS mice of 15 weeks old, in which a LOC of 0.5mm in diameter was made in the trochlear groove. Ten animals were sacrificed at day 7, and the other 10 animals at day 14 of follow up. To assess the ability of the animal to repair/regenerate, a histological analysis was performed using hematoxylin-eosin and safranin-O stains, and the results were evaluated by the ICRS scale using areas of healthy cartilage from the same joint as control. The Mann-Whitney U test was used for the statistical analyses of scores (averages).
  
  
  RESULTS
  Significant differences were found in days 7 and 14 between the LOC area and control areas, but no differences were found between the day 7 and day 14.
  
  
  CONCLUSIONS
  This model of LOC in the trochlear groove of adult mice is highly reproducible, and could be used in further studies to obtain better treatments for chondral pathologies.
authors.affiliation: Facultad de Medicina Clínica Alemana-Universidad del Desarrollo, Santiago, Chile. Electronic address: doctormartinez@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Martinez
authors.affiliation: Facultad de Medicina Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
authors.forename: D
authors.initials: D
authors.lastname: Figueroa
authors.affiliation: Facultad de Medicina Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
authors.forename: R
authors.initials: R
authors.lastname: Calvo
authors.affiliation: Facultad de Medicina Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
authors.forename: P
authors.initials: P
authors.lastname: Conget
authors.affiliation: Facultad de Medicina Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
authors.forename: M
authors.initials: M
authors.lastname: Gallegos
authors.affiliation: Facultad de Medicina Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
authors.forename: F
authors.initials: F
authors.lastname: Figueroa
authors.affiliation: Facultad de Medicina Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
authors.forename: X
authors.initials: X
authors.lastname: Ahumada
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000293:injuries* / Q000473:pathology / Q000502:physiology; D004551:Elbow Joint / Q000473:pathology / Q000502:physiology; D005260:Female; D006811:Humerus / Q000293:injuries* / Q000473:pathology / Q000502:physiology; D051379:Mice; D023421:Models, Animal; D000092464:Elbow Injuries
keywords: Animal model; Cartilage; Cartílago; Lesión osteocondral; Mice; Modelo animal; Osteochondral lesion; Ratón