title: ER stress responses in the absence of apoptosome: a comparative study in CASP9 proficient vs deficient mouse embryonic fibroblasts.
abstract: Cells respond to endoplasmic reticulum (ER) stress through the unfolded protein response (UPR), autophagy and cell death. In this study we utilized casp9(+/+) and casp9(-/-) MEFs to determine the effect of inhibition of mitochondrial apoptosis pathway on ER stress-induced-cell death, UPR and autophagy. We observed prolonged activation of UPR and autophagy in casp9(-/-) cells as compared with casp9(+/+) MEFs, which displayed transient activation of both pathways. Furthermore we showed that while casp9(-/-) MEFs were resistant to ER stress, prolonged exposure led to the activation of a non-canonical, caspase-mediated mode of cell death.
authors.affiliation: Apoptosis Research Centre, NUI Galway, Ireland; School of Natural Sciences, NUI Galway, Ireland.
authors.forename: Shane
authors.initials: S
authors.lastname: Deegan
authors.affiliation: Apoptosis Research Centre, NUI Galway, Ireland; School of Natural Sciences, NUI Galway, Ireland.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Saveljeva
authors.affiliation: Apoptosis Research Centre, NUI Galway, Ireland; School of Medicine, NUI Galway, Ireland.
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Apoptosis Research Centre, NUI Galway, Ireland; School of Natural Sciences, NUI Galway, Ireland.
authors.forename: David C
authors.initials: DC
authors.lastname: MacDonald
authors.affiliation: Apoptosis Research Centre, NUI Galway, Ireland; School of Natural Sciences, NUI Galway, Ireland. Electronic address: afshin.samali@nuigalway.ie.
authors.forename: Afshin
authors.initials: A
authors.lastname: Samali
mesh_terms: D000818:Animals; D053478:Apoptosomes / Q000172:deficiency; D001343:Autophagy / Q000502:physiology*; D053453:Caspase 9 / Q000172:deficiency / Q000502:physiology*; D016923:Cell Death / Q000502:physiology*; D059865:Endoplasmic Reticulum Stress; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice / Q000196:embryology; D008928:Mitochondria / Q000378:metabolism*; D056811:Unfolded Protein Response / Q000502:physiology
chemical_list: D053478:Apoptosomes; D053453:Caspase 9
keywords: Apoptosis; Autophagy; Caspase; Endoplasmic reticulum (ER) stress; Unfolded protein response (UPR)