title: Quorum sensing in Bacillus thuringiensis is required for completion of a full infectious cycle in the insect.
abstract: Bacterial cell-cell communication or quorum sensing (QS) is a biological process commonly described as allowing bacteria belonging to a same pherotype to coordinate gene expression to cell density. In Gram-positive bacteria, cell-cell communication mainly relies on cytoplasmic sensors regulated by secreted and re-imported signaling peptides. The Bacillus quorum sensors Rap, NprR, and PlcR were previously identified as the first members of a new protein family called RNPP. Except for the Rap proteins, these RNPP regulators are transcription factors that directly regulate gene expression. QS regulates important biological functions in bacteria of the Bacillus cereus group. PlcR was first characterized as the main regulator of virulence in B. thuringiensis and B. cereus. More recently, the PlcR-like regulator PlcRa was characterized for its role in cysteine metabolism and in resistance to oxidative stress. The NprR regulator controls the necrotrophic properties allowing the bacteria to survive in the infected host. The Rap proteins negatively affect sporulation via their interaction with a phosphorelay protein involved in the activation of Spo0A, the master regulator of this differentiation pathway. In this review we aim at providing a complete picture of the QS systems that are sequentially activated during the lifecycle of B. cereus and B. thuringiensis in an insect model of infection.
authors.affiliation: INRA, Unité MICALIS UMR-1319, La Minière, 78280 Guyancourt, France. leyla.slamti@jouy.inra.fr.
authors.forename: Leyla
authors.initials: L
authors.lastname: Slamti
authors.affiliation: INRA, Unité MICALIS UMR-1319, La Minière, 78280 Guyancourt, France. stephane.perchat@jouy.inra.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Perchat
authors.affiliation: INRA, Unité MICALIS UMR-1319, La Minière, 78280 Guyancourt, France. eugenie.huillet@jouy.inra.fr.
authors.forename: Eugénie
authors.initials: E
authors.lastname: Huillet
authors.affiliation: INRA, Unité MICALIS UMR-1319, La Minière, 78280 Guyancourt, France. didier.lereclus@jouy.inra.fr.
authors.forename: Didier
authors.initials: D
authors.lastname: Lereclus
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000472:pathogenicity / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D007313:Insecta / Q000382:microbiology*; D053038:Quorum Sensing; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors
references.citation: FEMS Microbiol Rev. 2008 Jul;32(4):579-606
references.pmid: 18422617
references.citation: Environ Microbiol. 2012 Aug;14(8):2233-46
references.pmid: 22540344
references.citation: Biotechnology (N Y). 1995 Jan;13(1):67-71
references.pmid: 9634751
references.citation: Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1549-53
references.pmid: 8643670
references.citation: Mol Microbiol. 1999 Jun;32(5):1043-53
references.pmid: 10361306
references.citation: Trends Microbiol. 2010 May;18(5):189-94
references.pmid: 20338765
references.citation: Mol Microbiol. 2002 Oct;46(2):297-304
references.pmid: 12406209
references.citation: J Bacteriol. 2006 Jan;188(2):487-98
references.pmid: 16385039
references.citation: Clin Microbiol Rev. 2010 Apr;23(2):382-98
references.pmid: 20375358
references.citation: J Bacteriol. 2003 Aug;185(16):4861-71
references.pmid: 12897006
references.citation: J Bacteriol. 2001 Aug;183(16):4905-9
references.pmid: 11466295
references.citation: Curr Opin Microbiol. 2005 Apr;8(2):203-7
references.pmid: 15802253
references.citation: Curr Opin Microbiol. 2000 Dec;3(6):561-6
references.pmid: 11121774
references.citation: Infect Immun. 2003 Jun;71(6):3116-24
references.pmid: 12761089
references.citation: J Biol Chem. 2002 Jun 7;277(23):20483-9
references.pmid: 11923303
references.citation: Mol Microbiol. 2001 May;40(4):963-75
references.pmid: 11401703
references.citation: PLoS Biol. 2013;11(3):e1001516
references.pmid: 23526885
references.citation: Cell. 1991 Feb 8;64(3):545-52
references.pmid: 1846779
references.citation: Appl Environ Microbiol. 2000 Jun;66(6):2627-30
references.pmid: 10831447
references.citation: Nucleic Acids Res. 2008 Jun;36(11):3791-801
references.pmid: 18492723
references.citation: Biochemistry. 2007 Mar 20;46(11):3234-45
references.pmid: 17323930
references.citation: J Bacteriol. 2003 Mar;185(6):1911-22
references.pmid: 12618455
references.citation: Mol Microbiol. 2013 Apr;88(1):48-63
references.pmid: 23388036
references.citation: Nat Methods. 2011 Sep 29;8(10):785-6
references.pmid: 21959131
references.citation: FEMS Microbiol Lett. 1994 Aug 1;121(1):31-4
references.pmid: 8082824
references.citation: Peptides. 2001 Oct;22(10):1541-7
references.pmid: 11587783
references.citation: PLoS Biol. 2013;11(3):e1001511
references.pmid: 23526880
references.citation: PLoS One. 2012;7(12):e51047
references.pmid: 23239999
references.citation: Proteomics. 2002 Jun;2(6):784-91
references.pmid: 12112862
references.citation: J Bacteriol. 1996 May;178(10):2749-56
references.pmid: 8631661
references.citation: Int J Med Microbiol. 2000 Oct;290(4-5):295-9
references.pmid: 11111901
references.citation: FEMS Microbiol Rev. 2014 May;38(3):473-92
references.pmid: 24118108
references.citation: Nucleic Acids Res. 2013 Sep;41(16):7920-33
references.pmid: 23793817
references.citation: Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18596-601
references.pmid: 16339309
references.citation: EMBO J. 2002 Sep 2;21(17):4550-9
references.pmid: 12198157
references.citation: Annu Rev Genet. 2008;42:541-64
references.pmid: 18713030
references.citation: BMC Microbiol. 2008 Oct 16;8:183
references.pmid: 18925929
references.citation: Proc Natl Acad Sci U S A. 1990 Mar;87(5):1801-5
references.pmid: 2106683
references.citation: Genes Dev. 2005 Sep 15;19(18):2236-44
references.pmid: 16166384
references.citation: Cell. 1997 Jun 13;89(6):917-25
references.pmid: 9200610
references.citation: Cold Spring Harb Perspect Med. 2012 Nov 01;2(11):
references.pmid: 23125205
references.citation: Hong Kong Med J. 2001 Mar;7(1):89-92
references.pmid: 11406681
references.citation: J Biol Chem. 2008 Dec 19;283(51):35551-60
references.pmid: 18974048
references.citation: J Bacteriol. 2006 May;188(10):3551-71
references.pmid: 16672610
references.citation: Mol Microbiol. 2003 Sep;49(6):1509-22
references.pmid: 12950917
references.citation: FEMS Immunol Med Microbiol. 2011 Jul;62(2):123-39
references.pmid: 21539625
references.citation: Mol Microbiol. 2011 Nov;82(3):619-33
references.pmid: 21958299
references.citation: Ann Thorac Surg. 1999 Dec;68(6):2351-2
references.pmid: 10617040
references.citation: Annu Rev Microbiol. 2009;63:575-97
references.pmid: 19575568
references.citation: Nat Chem Biol. 2009 Sep;5(9):625-7
references.pmid: 19578333
references.citation: PLoS Biol. 2011 Dec;9(12):e1001226
references.pmid: 22215984
references.citation: Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18490-5
references.pmid: 17998541
references.citation: Annu Rev Genet. 2009;43:197-222
references.pmid: 19686078
references.citation: Environ Microbiol. 2011 Jun;13(6):1387-94
references.pmid: 21352461
references.citation: Mol Microbiol. 2004 Oct;54(2):520-32
references.pmid: 15469521
references.citation: Plant Biotechnol J. 2011 Apr;9(3):283-300
references.pmid: 21375687
references.citation: J Bacteriol. 2005 Feb;187(3):1182-7
references.pmid: 15659693
references.citation: J Mol Biol. 1990 Oct 5;215(3):359-72
references.pmid: 2121995
references.citation: Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1047-52
references.pmid: 23277548
references.citation: J Mol Biol. 1997 Sep 19;272(2):200-12
references.pmid: 9299348
references.citation: Mol Microbiol. 2012 Jul;85(1):67-88
references.pmid: 22571587
references.citation: Mol Microbiol. 2007 Sep;65(5):1321-33
references.pmid: 17666034
references.citation: Nucleic Acids Res. 2007 Jul;35(Web Server issue):W429-32
references.pmid: 17483518
references.citation: Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12055-9
references.pmid: 8618843
references.citation: PLoS Pathog. 2012;8(4):e1002629
references.pmid: 22511867
references.citation: Science. 1984 Nov 16;226(4676):849-50
references.pmid: 6436978
references.citation: PLoS One. 2008 Jul 30;3(7):e2793
references.pmid: 18665214
references.citation: Microbiology (Reading). 2005 Jan;151(Pt 1):183-197
references.pmid: 15632437
references.citation: Mol Microbiol. 1996 Mar;19(6):1151-7
references.pmid: 8730857
references.citation: J Bacteriol. 1992 Jul;174(13):4361-73
references.pmid: 1378051
references.citation: PLoS Biol. 2013;11(3):e1001512
references.pmid: 23526881
references.citation: J Bacteriol. 2005 Oct;187(20):6972-81
references.pmid: 16199567
references.citation: Toxins (Basel). 2014 Jul 23;6(7):2194-209
references.pmid: 25055802
references.citation: Mol Microbiol. 2000 Nov;38(3):535-42
references.pmid: 11069677
references.citation: Clin Infect Dis. 2001 May 15;32(10):1456-62
references.pmid: 11317247
references.citation: Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8612-7
references.pmid: 9238025
references.citation: Microbiology (Reading). 2000 Nov;146 ( Pt 11):2825-2832
references.pmid: 11065361
references.citation: Structure. 2000 Aug 15;8(8):851-62
references.pmid: 10997904
references.citation: FEMS Immunol Med Microbiol. 2009 Apr;55(3):361-77
references.pmid: 19159431