title: Horseradish peroxidase microcapsules based on layer-by-layer polyelectrolyte deposition.
abstract: Polyelectrolyte multilayer microcapsules have been prepared for encapsulating horseradish peroxidase (HRP). The CaCO3 microparticles containing HRP molecules were prepared and subsequently enwrapped with layer-by-layer (LbL) deposited multilayer films. The LbL films were constructed via an alternate electrostatic adsorption of poly(allylamine) and poly(styrene sulfonate). The CaCO3 templates were then dissolved in an ethylenediaminetetraacetic acid solution, leaving HRP in the microcapsules. Optical microscope and fluorescence microscope observations indicated that the microcapsules are homogeneous spheres with an average diameter about 7 μm. The encapsulated HRP showed catalytic activity in the oxidation of pyrogallol, suggesting the microcapsules provide a suitable environment for enzymatic reaction. A leakage of HRP from the microcapsules was negligibly small. Therefore, the HRP-containing microcapsules is promising for detection and treatment of phenolic compounds.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan; The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. zhaozixia@hotmail.com
authors.forename: Zixia
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Anzai
mesh_terms: D055162:Biocatalysis; D002214:Capsules; D004573:Electrolytes / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D058525:Quartz Crystal Microbalance Techniques; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002214:Capsules; D004573:Electrolytes; D006046:Gold; D006735:Horseradish Peroxidase; D016650:Fluorescein-5-isothiocyanate