title: Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis.
abstract: A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mg L(-1) for glucose to 0.85 mg L(-1) for lactate.
authors.affiliation: Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Podolec
authors.affiliation: Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
authors.forename: Alexandra Hengerics
authors.initials: AH
authors.lastname: Szabó
authors.affiliation: Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Blaško
authors.affiliation: Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia. Electronic address: kubinec@fns.uniba.sk.
authors.forename: Róbert
authors.initials: R
authors.lastname: Kubinec
authors.affiliation: Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia.
authors.forename: Renáta
authors.initials: R
authors.lastname: Górová
authors.affiliation: Institute of Chemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-2, 842 15 Bratislava, Slovakia; SynthCluster, s.r.o., Komenského 1439, 900 01 Modra, Slovakia.
authors.forename: Jozef
authors.initials: J
authors.lastname: Višňovský
authors.affiliation: Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-1, 842 15 Bratislava, Slovakia.
authors.forename: Anna
authors.initials: A
authors.lastname: Gnipová
authors.affiliation: Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina CH-1, 842 15 Bratislava, Slovakia.
authors.forename: Anton
authors.initials: A
authors.lastname: Horváth
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Bierhanzl
authors.affiliation: Institute of Forensic Medicine and Toxicology, General University Hospital in Prague, U Nemocnice 2, 128 08 Prague 2, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Hložek
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic; Institute of Forensic Medicine and Toxicology, General University Hospital in Prague, U Nemocnice 2, 128 08 Prague 2, Czech Republic.
authors.forename: Radomír
authors.initials: R
authors.lastname: Čabala
mesh_terms: D000409:Alanine / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005947:Glucose / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D017646:Organosilicon Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D014297:Trimethylsilyl Compounds / Q000737:chemistry; D014346:Trypanosoma brucei brucei / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D017646:Organosilicon Compounds; D014297:Trimethylsilyl Compounds; D019344:Lactic Acid; C047270:N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide; C024548:hexamethylsilazane; D005947:Glucose; D000409:Alanine
keywords: Aqueous samples; GC–MS/MS analysis; Metabolites; Protozoa; Trimethylsilylation