title: Renal response to L-arginine in diabetic rats. A possible link between nitric oxide system and aquaporin-2.
abstract: The aim of this study was to evaluate whether L-Arginine (L-Arg) supplementation modifies nitric oxide (NO) system and consequently aquaporin-2 (AQP2) expression in the renal outer medulla of streptozotocin-diabetic rats at an early time point after induction of diabetes. Male Wistar rats were divided in four groups: Control, Diabetic, Diabetic treated with L-Arginine and Control treated with L-Arginine. Nitric oxide synthase (NOS) activity was estimated by [14C] L-citrulline production in homogenates of the renal outer medulla and by NADPH-diaphorase staining in renal outer medullary tubules. Western blot was used to detect the expression of AQP2 and NOS types I and III; real time PCR was used to quantify AQP2 mRNA. The expression of both NOS isoforms, NOS I and NOS III, was decreased in the renal outer medulla of diabetic rats and L-Arg failed to prevent these decreases. However, L-Arg improved NO production, NADPH-diaphorase activity in collecting ducts and other tubular structures, and NOS activity in renal homogenates from diabetic rats. AQP2 protein and mRNA were decreased in the renal outer medulla of diabetic rats and L-Arg administration prevented these decreases. These results suggest that the decreased NOS activity in collecting ducts of the renal outer medulla may cause, at least in part, the decreased expression of AQP2 in this model of diabetes and constitute additional evidence supporting a role for NO in contributing to renal water reabsorption through the modulation of AQP2 expression in this pathological condition. However, we cannot discard that another pathway different from NOS also exists that links L-Arg to AQP2 expression.
authors.affiliation: Cátedra de Biología Celular y Molecular, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Buenos Aires, Argentina.
authors.forename: María C
authors.initials: MC
authors.lastname: Ortiz
authors.affiliation: Cátedra de Biología Celular y Molecular, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Buenos Aires, Argentina.
authors.forename: María F
authors.initials: MF
authors.lastname: Albertoni Borghese
authors.affiliation: Cátedra de Biología Celular y Molecular, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Buenos Aires, Argentina.
authors.forename: Sabrina E
authors.initials: SE
authors.lastname: Balonga
authors.affiliation: Cátedra de Biología Celular y Molecular, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Buenos Aires, Argentina.
authors.forename: Agustina
authors.initials: A
authors.lastname: Lavagna
authors.affiliation: Cátedra de Biología Celular y Molecular, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Buenos Aires, Argentina.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Filipuzzi
authors.affiliation: Cátedra de Fisiología, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Buenos Aires, Argentina.
authors.forename: Rosana
authors.initials: R
authors.lastname: Elesgaray
authors.affiliation: Cátedra de Fisiología, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Buenos Aires, Argentina.
authors.forename: María A
authors.initials: MA
authors.lastname: Costa
authors.affiliation: Cátedra de Biología Celular y Molecular, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Buenos Aires, Argentina.
authors.forename: Mónica P
authors.initials: MP
authors.lastname: Majowicz
mesh_terms: D000818:Animals; D051399:Aquaporin 2 / Q000096:biosynthesis* / Q000378:metabolism; D001120:Arginine / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects; D002956:Citrulline / Q000096:biosynthesis; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000473:pathology*; D007679:Kidney Medulla / Q000473:pathology; D007685:Kidney Tubules, Collecting / Q000473:pathology; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
chemical_list: C496025:Aqp2 protein, rat; D051399:Aquaporin 2; D001786:Blood Glucose; D002956:Citrulline; D009569:Nitric Oxide; D013311:Streptozocin; D001120:Arginine; D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III; D009252:NADPH Dehydrogenase
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