title: [Pharmacokinetic study of six aconitine alkaloids in aconiti lateralis radix praeparata in beagle dogs].
abstract: OBJECTIVE
  To study the pharmacokinetics characteristics of six Aconitum alkaloids aconitine (AC), mesaconitine (MA), hypaconitine (HA), benzoylaconine (BAC), benzoylmesaconine (BMA) and benzoylhypaconine (BHA) in beagle dogs.
  
  
  METHODS
  An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for simultaneous quantitation of six Aconitum alkaloids in beagle dog plasma after oral administration of Aconiti Lateralis Radix Praeparata decoction. UPLC/MS/MS system coupled with an electrospray ionization (ESI) source was performed in multiple-reaction monitoring (MRM) mode. Sample preparation was performed with solid-phase extraction(SPE) on a 3 mL HLB cartridge before the analysis. The separation was applied on a Waters C8 column (100 mm x 2.1 mm, 1.7 microm) and a gradient elution of methanol and 0.2% formic acid-water was used as mobile phase. The pharmacokinetic parameters were calculated by the results of the analysis through the DAS 2. 1 software (Drug and Statistics for Windows).
  
  
  RESULTS
  The results showed that the fitting model for the six Aconitum alkaloids was the one-compartment model pharmacokinetics.
  
  
  CONCLUSIONS
  The method is successfully used for the pharmacokinetic evaluation of the six Aconitum alkaloids in beagle dog plasma, it can help monitor the ADME/Tox process when taking Aconiti Lateralis Radix Praeparata by observing the pharmacokinetic process. The results provide a good reference for clinical treatment and safe application of Aconiti Lateralis Radix Praeparata.
authors.forename: Ri-Ping
authors.initials: RP
authors.lastname: Xiao
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Lai
authors.forename: Yai
authors.initials: Y
authors.lastname: Zhao
authors.forename: Liang-Wen
authors.initials: LW
authors.lastname: Yu
authors.forename: Yue-Lan
authors.initials: YL
authors.lastname: Zhu
authors.forename: Geng
authors.initials: G
authors.lastname: Li
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics*; D031841:Aconitum / Q000737:chemistry*; D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D016014:Linear Models; D008297:Male; D018517:Plant Roots / Q000737:chemistry; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D000157:Aconitine