title: Synthesis, physiochemical characterization, and biocompatibility of a chitosan/dextran-based hydrogel for postsurgical adhesion prevention.
abstract: An amine-functionalized succinyl chitosan and an oxidized dextran were synthesized and mixed in aqueous solution to form an in situ chitosan/dextran injectable, surgical hydrogel for adhesion prevention. Rheological characterization showed that the rate of gelation and moduli were tunable based on amine and aldehyde levels, as well as polymer concentrations. The CD hydrogels have been shown to be effective post-operative aids in prevention of adhesions in ear, nose, and throat surgeries and abdominal surgeries in vivo. In vitro biocompatibility testing was performed on CD hydrogels containing one of two oxidized dextrans, an 80 % oxidized (CD-100) or 25 % (CD-25) oxidized dextran. However, the CD-100 hydrogel showed moderate cytotoxicity in vitro to Vero cells. SC component of the CD hydrogel, however, showed no cytotoxic effect. In order to increase the biocompatibility of the hydrogel, a lower aldehyde level hydrogel was developed. CD-25 was found to be non-cytotoxic to L929 fibroblasts. The in vivo pro-inflammatory response of the CD-25 hydrogel, after intraperitoneal injection in BALB/c mice, was also determined by measuring serum TNF-α levels and by histological analysis of tissues. TNF-α levels were similar in mice injected with CD-25 hydrogel as compared to the negative saline injected control; and were significantly different (P < 0.05) as compared to the positive, lipopolysaccharide, injected control. Histological examination revealed no inflammation seen in CD hydrogel injected mice. The results of these in vitro and in vivo studies demonstrate the biocompatibility of the CD hydrogel as a post-operative aid for adhesion prevention.
authors.affiliation: Department of Chemistry, University of Otago, Dunedin, 9054, New Zealand, jcabral@chemistry.otago.ac.nz.
authors.forename: Jaydee D
authors.initials: JD
authors.lastname: Cabral
authors.forename: Marina
authors.initials: M
authors.lastname: Roxburgh
authors.forename: Zheng
authors.initials: Z
authors.lastname: Shi
authors.forename: Liqi
authors.initials: L
authors.lastname: Liu
authors.forename: Michelle
authors.initials: M
authors.lastname: McConnell
authors.forename: Gail
authors.initials: G
authors.lastname: Williams
authors.forename: Natasha
authors.initials: N
authors.lastname: Evans
authors.forename: Lyall R
authors.initials: LR
authors.lastname: Hanton
authors.forename: Jim
authors.initials: J
authors.lastname: Simpson
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Moratti
authors.forename: Brian H
authors.initials: BH
authors.lastname: Robinson
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Wormald
authors.forename: Simon
authors.initials: S
authors.lastname: Robinson
mesh_terms: D000818:Animals; D047070:Bandages, Hydrocolloid; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology*; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D002522:Chlorocebus aethiops; D003911:Dextrans / Q000737:chemistry* / Q000494:pharmacology; D055119:Elastic Modulus; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006244:Hardness; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D000267:Tissue Adhesions / Q000517:prevention & control*; D014709:Vero Cells
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D020100:Hydrogels; D048271:Chitosan
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