title: Comparison of complete nucleotide sequences and genome organization of six distinct cherry leaf roll virus isolates from New Zealand.
abstract: The complete genomic sequences of six phenotypically distinct cherry leaf roll virus (CLRV) isolates from New Zealand were determined and compared. RNA1 and RNA2 are 7,919-7,921 and 6,361-6,363 nucleotides in length, respectively, excluding the poly(A) tails. Both genome segments contain a single open reading frame and encode one polyprotein of 2,113 amino acids for RNA1 and 1,590 aa for RNA2, with corresponding molecular masses of 235.5-236.1 kDa and 174.2-174.8 kDa, respectively. The six RNA1 sequences share 92.0-99.7 % nt and 97.0-99.5 % aa similarity, and the RNA2 sequences share 91.4-99.8 % nt and 94.3-99.7 % aa similarity. Phylogenetic analysis established close associations between the six New Zealand CLRV isolates and members of the subgroup C nepoviruses.
authors.affiliation: School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand, ewoo040@aucklanduni.ac.nz.
authors.forename: E N Y
authors.initials: EN
authors.lastname: Woo
authors.forename: M N
authors.initials: MN
authors.lastname: Pearson
mesh_terms: D016000:Cluster Analysis; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017860:Nepovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009520:New Zealand; D016366:Open Reading Frames; D010802:Phylogeny; D018515:Plant Leaves / Q000821:virology; D020815:Polyproteins / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D026801:Synteny; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins