title: Effect of particle size on the speed and resolution of chiral separations using supercritical fluid chromatography.
abstract: Fast chiral supercritical fluid chromatography (SFC) separations have become important due to the increasing use of high-throughput experimentation (HTE) in organic synthesis. These HTE experiments can generate hundreds of samples for chiral analysis that need to be assayed in a short time. In general, chiral SFC can provide much faster analysis times compared to liquid chromatography (LC). Additionally, columns packed with smaller particles can provide faster and more efficient separations. In this study, the effect of the particle size on the speed and resolution of chiral separations by SFC was evaluated. The performance of Chiralcel OD columns packed with either 5 or 3 μm particles were compared using van Deemter or other kinetic plots. The benefits of using smaller particle columns for chiral SFC analysis are illustrated.
authors.affiliation: Department of Analytical Chemistry, Merck Research Laboratories, Rahway, New Jersey 07065, USA. Electronic address: mirlinda_biba@merck.com.
authors.forename: Mirlinda
authors.initials: M
authors.lastname: Biba
authors.affiliation: Department of Analytical Chemistry, Merck Research Laboratories, Rahway, New Jersey 07065, USA. Electronic address: erik.regalado@merck.com.
authors.forename: Erik L
authors.initials: EL
authors.lastname: Regalado
authors.affiliation: Department of Analytical Chemistry, Merck Research Laboratories, Rahway, New Jersey 07065, USA; Analytical Research and Development, Celgene Corporation, 86 Morris Avenue, Summit, New Jersey 07901, USA.
authors.forename: Naijun
authors.initials: N
authors.lastname: Wu
authors.affiliation: Department of Analytical Chemistry, Merck Research Laboratories, Rahway, New Jersey 07065, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Welch
mesh_terms: D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D007700:Kinetics; D010316:Particle Size
keywords: Chiral separation; Fast separations.; High-throughput analysis; Small particle size; Supercritical fluid chromatography