title: Clonality and Resistome analysis of KPC-producing Klebsiella pneumoniae strain isolated in Korea using whole genome sequencing.
abstract: We analyzed the whole genome sequence and resistome of the outbreak Klebsiella pneumoniae strain MP14 and compared it with those of K. pneumoniae carbapenemase- (KPC-) producing isolates that showed high similarity in the NCBI genome database. A KPC-2-producing multidrug-resistant (MDR) K. pneumoniae clinical isolate was obtained from a patient admitted to a Korean hospital in 2011. The strain MP14 was resistant to all tested β-lactams including monobactam, amikacin, levofloxacin, and cotrimoxazole, but susceptible to tigecycline and colistin. Resistome analysis showed the presence of β-lactamase genes including bla KPC-2, bla SHV-11, bla TEM-169, and bla OXA-9. MP14 also possessed aac(6'-)Ib, aadA2, and aph(3'-)Ia as aminoglycoside resistance-encoding genes, mph(A) for macrolides, oqxA and oqxB for quinolone, catA1 for phenicol, sul1 for sulfonamide, and dfrA12 for trimethoprim. Both SNP tree and cgMLST analysis showed the close relatedness with the KPC producers (KPNIH strains) isolated from an outbreak in the USA and colistin-resistant strains isolated in Italy. The plasmid-scaffold genes in plasmids pKpQil, pKpQil-IT, pKPN3, or pKPN-IT were identified in MP14, KPNIH, and Italian strains. The KPC-2-producing MDR K. pneumoniae ST258 stain isolated in Korea was highly clonally related with MDR K. pneumoniae strains from the USA and Italy. Global spread of KPC-producing K. pneumoniae is a worrying phenomenon.
authors.affiliation: Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Yangsoon
authors.identifier: 0000-0003-1220-5372
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Chunlab, Inc., Seoul National University, Republic of Korea.
authors.forename: Bong-Soo
authors.identifier: 0000-0003-1243-8280
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Chunlab, Inc., Seoul National University, Republic of Korea ; School of Biological Sciences, Seoul National University, Republic of Korea.
authors.forename: Jongsik
authors.identifier: 0000-0003-3385-5171
authors.initials: J
authors.lastname: Chun
authors.affiliation: Department of Science, University of British Columbia, Vancouver, Canada.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Yong
authors.affiliation: Division of Antimicrobial Resistance, Korea National Institute of Health, Osong, Republic of Korea.
authors.forename: Yeong Seon
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Division of Antimicrobial Resistance, Korea National Institute of Health, Osong, Republic of Korea.
authors.forename: Jung Sik
authors.initials: JS
authors.lastname: Yoo
authors.affiliation: Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Dongeun
authors.initials: D
authors.lastname: Yong
authors.affiliation: Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Republic of Korea.
authors.forename: Seong Geun
authors.initials: SG
authors.lastname: Hong
authors.affiliation: Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Roshan
authors.initials: R
authors.lastname: D'Souza
authors.affiliation: Department of Medical Microbiology and Immunology, Center for Research in Anti-Infectives and Biotechnology, Creighton University School of Medicine, Omaha, NE, USA.
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Thomson
authors.affiliation: Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors.forename: Yunsop
authors.initials: Y
authors.lastname: Chong
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D003428:Cross Infection / Q000453:epidemiology / Q000235:genetics; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D055106:Genome-Wide Association Study; D006801:Humans; D007558:Italy; D007710:Klebsiella Infections / Q000453:epidemiology / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification; D056910:Republic of Korea / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D014481:United States; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C531581:carbapenemase-2, Klebsiella pneumoniae
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