title: Short time impact of different hydroxyethyl starch solutions on the mesenteric microcirculation in experimental sepsis in rats.
abstract: BACKGROUND
  Fluid resuscitation plays a crucial role in the therapy of severe sepsis and septic shock. The use of colloids in sepsis is controversial at present. The aim of our study was to evaluate the effects of second and third generation colloids on the mesenteric microcirculation in early experimental sepsis.
  
  
  METHODS
  Male Lewis rats (n=64) were used. Animals underwent sham surgery or colon ascendens stent insertion for sepsis induction by peritonitis. Sixteen hours after the surgery animals were randomly assigned to receive one of the following fluid regimens intravenously: 16ml/kg Ringer's lactate, 64ml/kg Ringer's lactate, 16ml/kg 130/0.4 hydroxyethyl starch, and 16ml/kg 200/0.5 hydroxyethyl starch. Intravital microscopy of the mesenteric microcirculation (plasma extravasation; leukocyte-endothelial interactions) and arterial blood gas analysis were performed before and after fluid resuscitation.
  
  
  RESULTS
  In animals with experimental sepsis plasma extravasation was significantly increased compared to control animals (p<0.05). There were no significant differences in plasma extravasation between septic animals receiving crystalloids and or colloid. Furthermore, the type of administered fluid did not influence the number of adhering leucocytes during the observation period.
  
  
  CONCLUSIONS
  The short time impact of different hydroxyethyl starch solutions on the microcirculation of the mesentery is not different from crystalloids in colon ascendens stent peritonitis-induced experimental sepsis in rats.
authors.affiliation: Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Wafa
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Ernst Moritz Arndt University Greifswald, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Herrmann
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Ernst Moritz Arndt University Greifswald, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Kuhnert
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Ernst Moritz Arndt University Greifswald, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Wegner
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Ernst Moritz Arndt University Greifswald, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Gründling
authors.affiliation: Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada; Department of Anesthesiology and Intensive Care Medicine, Ernst Moritz Arndt University Greifswald, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Pavlovic
authors.affiliation: Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada. Electronic address: chlehmann@dal.ca.
authors.forename: C
authors.initials: C
authors.lastname: Lehmann
mesh_terms: D000818:Animals; D002196:Capillaries / Q000187:drug effects* / Q000276:immunology / Q000503:physiopathology; D002199:Capillary Permeability / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000187:drug effects / Q000276:immunology; D005440:Fluid Therapy / Q000379:methods*; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology*; D007262:Infusions, Intravenous; D007552:Isotonic Solutions / Q000008:administration & dosage; D036904:Leukocyte Rolling / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D008833:Microcirculation / Q000187:drug effects*; D010952:Plasma Substitutes / Q000494:pharmacology*; D011917:Rats, Inbred Lew; D000077325:Ringer's Lactate; D018805:Sepsis / Q000188:drug therapy* / Q000276:immunology / Q000503:physiopathology; D013152:Splanchnic Circulation / Q000187:drug effects*; D013997:Time Factors
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D007552:Isotonic Solutions; D010952:Plasma Substitutes; D000077325:Ringer's Lactate
keywords: Colloids; Crystalloids; Fluid extravasation; Hydroxyethyl starch; Intravital microscopy; Leukocyte adherence; Sepsis