title: Important sequence for overexpression of NADH oxidase gene from Thermus thermophilus HB8 in Escherichia coli.
abstract: Enzymes fixed on the electrode of biosensor are gradually inactivated and the electrode is discarded after using several times. In order to prepare the stable biosensor, we try to use a stable enzyme from extreme thermophilic bacteria, Thermus thermophilus HB8. It is very important that a stable enzyme from T. thermophilus HB8 is overproduced in Escherichia coli, for the purpose of enough supply of enzyme. Thereby, we determined the important sequence for overexpression of NADH oxidase (nox) gene from T. thermophilus HB8 in E. coli. As a result, it is revealed that ten nucleotides sequence, GAAATTAACT, in the upstream of start codon of nox gene was important for its overexpression in E. coli.
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.
authors.forename: Norikazu
authors.initials: N
authors.lastname: Matsumura
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohoka
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Sakuma
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Nakahata
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan; Department of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Ishikawa
mesh_terms: D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors / Q000378:metabolism; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D012995:Solubility; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics*
chemical_list: D009097:Multienzyme Complexes; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases