title: Antioxidant properties of mushroom mycelia obtained by batch cultivation and tocopherol content affected by extraction procedures.
abstract: The determination of the antioxidant potential of lyophilized mushroom mycelia from 5 strains of the species Pleurotus ostreatus and Coprinus comatus (obtained by submerged cultivation in batch system) was analyzed as ethanolic extracts by evaluating ABTS and the hydroxyl scavenging activity, FRAP method, the chelating capacity, the inhibition of human erythrocyte hemolysis, and the inhibition of xanthine oxidase activity. The main compounds present in all extracts were determined by HPLC chromatography. Overall, results demonstrated that the biologically active substances content is modulated by the extraction method used. The most beneficial extract, characterized by determining the EC50 value, was that of C. comatus M8102, followed by P. ostreatus PQMZ91109. Significant amount of α-tocopherol (179.51 ± 1.51 mg/100 g extract) was determined as well as flavones such as rutin and apigenin. In the P. ostreatus PQMZ91109 extract, 4.8 ± 0.05 mg/100 g extract of tocopherol acetate known to play a significant role as an antioxidant in skin protection against oxidative stress generated by UV rays was determined. The various correlations (r (2) = 0.7665-0.9426 for tocopherol content) assessed and the composition of extracts in fluidized bed from the mycelia of the tested species depicted a significant pharmacological potential as well as the possibility of usage in the development of new functional products.
authors.affiliation: Department of Industrial Biotechnology, University of Agronomical Sciences and Veterinary Medicine Bucharest, Faculty of Biotechnology, 59 Marasti Boulevard, 011464 Bucharest, Romania.
authors.forename: Emanuel
authors.identifier: 0000-0002-3376-2058
authors.initials: E
authors.lastname: Vamanu
mesh_terms: D000363:Agaricales / Q000737:chemistry; D047310:Apigenin / Q000302:isolation & purification / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D003302:Coprinus / Q000737:chemistry; D000431:Ethanol; D006801:Humans; D025282:Mycelium / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D020076:Pleurotus / Q000737:chemistry; D012431:Rutin / Q000302:isolation & purification / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects; D024505:Tocopherols / Q000302:isolation & purification* / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D010936:Plant Extracts; D000431:Ethanol; D012431:Rutin; D047310:Apigenin; D024505:Tocopherols
references.citation: Phytomedicine. 2004;11(4):309-14
references.pmid: 15185843
references.citation: Molecules. 2012 Mar 26;17(4):3653-71
references.pmid: 22450678
references.citation: Int J Mol Sci. 2011;12(6):4120-31
references.pmid: 21747728
references.citation: Biomed Res Int. 2013;2013:313905
references.pmid: 23509707
references.citation: Int J Med Chem. 2012;2012:157125
references.pmid: 25374682
references.citation: Food Chem. 2012 Dec 1;135(3):1220-5
references.pmid: 22953846
references.citation: J Biomed Biotechnol. 2012;2012:565974
references.pmid: 22778553
references.citation: J Agric Food Chem. 2008 Oct 8;56(19):9225-9
references.pmid: 18778031
references.citation: J Assoc Physicians India. 2004 Oct;52:794-804
references.pmid: 15909857
references.citation: Food Chem Toxicol. 2009 Sep;47(9):2381-8
references.pmid: 19563856
references.citation: Molecules. 2009 Nov 06;14(11):4476-85
references.pmid: 19924080
references.citation: Pak J Pharm Sci. 2008 Jul;21(3):249-54
references.pmid: 18614420
references.citation: Malays J Nutr. 2010 Aug;16(2):281-91
references.pmid: 22691933