title: Oxidative stress-dependent activation of collagen synthesis is induced in human pulmonary smooth muscle cells by sera from patients with scleroderma-associated pulmonary hypertension.
abstract: Pulmonary arterial hypertension is a major complication of systemic sclerosis. Although oxidative stress, intima hyperplasia and a progressive vessel occlusion appear to be clearly involved, the fine molecular mechanisms underpinning the onset and progression of systemic sclerosis-associated pulmonary arterial hypertension remain largely unknown. Here we shows for the first time that an increase of NADPH-derived reactive oxygen species production induced by sera from systemic sclerosis patients with pulmonary arterial hypertension drives collagen type I promoter activity in primary human pulmonary artery smooth muscle cells, suggesting that antioxidant-based therapies should be considered in the treatment of systemic sclerosis-associated vascular diseases.
authors.forename: Francesco
authors.initials: F
authors.lastname: Boin
authors.forename: Gian Luca
authors.initials: GL
authors.lastname: Erre
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Posadino
authors.forename: Annalisa
authors.initials: A
authors.lastname: Cossu
authors.forename: Roberta
authors.initials: R
authors.lastname: Giordo
authors.forename: Gaia
authors.initials: G
authors.lastname: Spinetti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Passiu
authors.forename: Costanza
authors.initials: C
authors.lastname: Emanueli
authors.affiliation: Laboratory of Vascular Biology, Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy. gpintus@uniss.it.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Pintus
mesh_terms: D003094:Collagen / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D006976:Hypertension, Pulmonary / Q000097:blood / Q000209:etiology*; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress; D045743:Scleroderma, Diffuse / Q000097:blood / Q000150:complications*
chemical_list: D003094:Collagen
references.citation: J Biol Chem. 1994 Apr 29;269(17):12684-91
references.pmid: 8175678
references.citation: Clin Chest Med. 2013 Dec;34(4):639-50
references.pmid: 24267295
references.citation: J Rheumatol. 1999 Oct;26(10):2159-67
references.pmid: 10529133
references.citation: Arthritis Rheum. 2013 Aug;65(8):1953-62
references.pmid: 23666787
references.citation: Arthritis Rheum. 1980 May;23(5):581-90
references.pmid: 7378088
references.citation: Chest. 2013 Oct;144(4):1346-1356
references.pmid: 24081346
references.citation: Antioxid Redox Signal. 2015 Aug 10;23(5):406-27
references.pmid: 24383718
references.citation: Open Rheumatol J. 2012;6:87-95
references.pmid: 22802906
references.citation: Physiol Rev. 2004 Jul;84(3):767-801
references.pmid: 15269336
references.citation: Toxicol Sci. 2010 Mar;114(1):101-12
references.pmid: 20015842