title: An16-resilin: an advanced multi-stimuli-responsive resilin-mimetic protein polymer.
abstract: Engineered protein polymers that display responsiveness to multiple stimuli are emerging as a promising class of soft material with unprecedented functionality. The remarkable advancement in genetic engineering and biosynthesis has created the opportunity for precise control over the amino acid sequence, size, structure and resulting functions of such biomimetic proteins. Herein, we describe the multi-stimuli-responsive characteristics of a resilin-mimetic protein, An16-resilin (An16), derived from the consensus sequence of resilin gene in the mosquito Anopheles gambiae. We demonstrate that An16 is an intrinsically disordered protein that displays unusual dual-phase thermal transition behavior along with responsiveness to pH, ion, light and humidity. Identifying the molecular mechanisms that allow An16 to sense and switch in response to varying environments furthers the ability to design intelligent biomacromolecules.
authors.affiliation: Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA 5095, Australia.
authors.forename: Rajkamal
authors.initials: R
authors.lastname: Balu
authors.affiliation: Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA 5095, Australia. Electronic address: naba.dutta@unisa.edu.au.
authors.forename: Naba K
authors.initials: NK
authors.lastname: Dutta
authors.affiliation: Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA 5095, Australia. Electronic address: namita.choudhury@unisa.edu.au.
authors.forename: Namita R
authors.initials: NR
authors.lastname: Choudhury
authors.affiliation: CSIRO Agriculture, Level 6, Queensland Bioscience Precinct, St Lucia, Queensland 4067, Australia.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Elvin
authors.affiliation: CSIRO Agriculture, Level 6, Queensland Bioscience Precinct, St Lucia, Queensland 4067, Australia.
authors.forename: Russell E
authors.initials: RE
authors.lastname: Lyons
authors.affiliation: ANSTO, Private Mail Bag, Kirrawee, New South Wales 2232, Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: Knott
authors.affiliation: CSIRO Manufacturing, Clayton, Victoria 3168, Australia.
authors.forename: Anita J
authors.initials: AJ
authors.lastname: Hill
mesh_terms: D000818:Animals; D000852:Anopheles / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D006813:Humidity; D057446:Hydrodynamics; D006863:Hydrogen-Ion Concentration; D019476:Insect Proteins / Q000737:chemistry*; D007477:Ions; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D011487:Protein Conformation; D053838:Scattering, Small Angle; D012996:Solutions; D055672:Static Electricity; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D019476:Insect Proteins; D007477:Ions; D011108:Polymers; D012996:Solutions; C030951:resilin
keywords: An16-resilin; Biomimetic protein; Intrinsically disordered protein (IDP); Multi-stimuli responsiveness; Self-assembly