title: Molecular basis of RNA polymerase promoter specificity switch revealed through studies ofThermusbacteriophage transcription regulator.
abstract: Transcription initiation is the central point of gene expression regulation. Understanding of molecular mechanism of transcription regulation requires, ultimately, the structural understanding of consequences of transcription factors binding to DNA-dependent RNA polymerase (RNAP), the enzyme of transcription. We recently determined a structure of a complex between transcription factor gp39 encoded by aThermusbacteriophage andThermusRNAP holoenzyme. In this addendum to the original publication, we highlight structural insights that explain the ability of gp39 to act as an RNAP specificity switch which inhibits transcription initiation from a major class of bacterial promoters, while allowing transcription from a minor promoter class to continue.
authors.affiliation: Waksman Institute; Rutgers; The State University of New Jersey; Piscataway, NJ USA ; St. Petersburg Polytechnical State University; St. Petersburg, Russia ; Skolkovo Institute of Science and Technology; Skolkovo, Russia.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Severinov
authors.affiliation: Waksman Institute; Rutgers; The State University of New Jersey; Piscataway, NJ USA.
authors.forename: Leonid
authors.initials: L
authors.lastname: Minakhin
authors.affiliation: RIKEN Systems and Structural Biology Center; Tsurumi-ku, Yokohama Japan ; Division of Structural and Synthetic Biology; RIKEN Center for Life Science Technologies; Tsurumi-ku, Yokohama Japan.
authors.forename: Shun-Ichi
authors.initials: SI
authors.lastname: Sekine
authors.affiliation: St. Petersburg Polytechnical State University; St. Petersburg, Russia ; Institutes of Gene Biology and Molecular Genetics; Russian Academy of Sciences; Moscow, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Lopatina
authors.affiliation: RIKEN Systems and Structural Biology Center; Tsurumi-ku, Yokohama Japan ; RIKEN Structural Biology Laboratory; Tsurumi-ku, Yokohama Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
keywords: bacterial RNA polymerase; bacteriophage; inhibitor; sigma factor; transcription regulation
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