title: [Establishment of transformation system of Lonicera macranthoides mediated by Agrobacterium tumefaciens].
abstract: OBJECTIVE
  Based on the system of regeneration,the genetic transformation system of Lonicera macranthoides was established.
  
  
  METHODS
  Tissue culture method of seedlings, Agrobacterium tumefaciens mediated transformation method of explants, report gene was detected by gus staining and PCR.
  
  
  RESULTS
  The efficient transformation time was 8 minutes of infection. The good transformation rate was gained with the kanamycin 35 mg/L and cefotaxime 600 mg/L. The concentration of kanamycin had a leading effect on bud differentiation between two antibiotics, and bud induction rate reached extremely significant difference. Results of gus staining and PCR proved that the gus gene was integrated into Lonicera macranthoides genome.
  
  
  CONCLUSIONS
  The genetic transformation system of Lonicera macranthoides leaves mediated by Agrobacterium tumefaciens EHAlO5 was established for the first time.
authors.forename: Guo-hua
authors.initials: GH
authors.lastname: Liu
authors.forename: Yi-qing
authors.initials: YQ
authors.lastname: Liu
authors.forename: Ze-xiong
authors.initials: ZX
authors.lastname: Chen
authors.forename: Zhou-jiang
authors.initials: ZJ
authors.lastname: Liu
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D002439:Cefotaxime / Q000494:pharmacology; D005822:Genetic Vectors; D007612:Kanamycin / Q000494:pharmacology; D031239:Lonicera / Q000235:genetics* / Q000254:growth & development; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D046509:Tissue Culture Techniques / Q000379:methods; D014170:Transformation, Genetic
chemical_list: D007612:Kanamycin; D002439:Cefotaxime