title: Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides.
abstract: We demonstrate examples of cellular differentiation assessments, including cellular neurite outgrowth and fat cell maturation, by measuring the degree of membrane adsorption or cellular internalization using designed peptides. Because changes in the cellular membrane and cytosol during differentiation were shown to influence membrane adsorption and cellular internalization, we could successfully evaluate the extent of differentiation simply like stain indicators.
authors.affiliation: Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259 B-40, Midori-ku, Yokohama 226-8501, Japan; Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Nagatsuta-cho 4259 G1-13, Midori-ku, Yokohama 226-8502, Japan; FIRST (Faculty of Frontiers of Innovative Research in Science and Technology), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan. Electronic address: kusui@center.konan-u.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Usui
authors.affiliation: Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259 B-40, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Kikuchi
authors.affiliation: Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259 B-40, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Kikuchi
authors.affiliation: Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Nagatsuta-cho 4259 G1-13, Midori-ku, Yokohama 226-8502, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Mie
authors.affiliation: Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Nagatsuta-cho 4259 G1-13, Midori-ku, Yokohama 226-8502, Japan.
authors.forename: Eiry
authors.initials: E
authors.lastname: Kobatake
authors.affiliation: Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259 B-40, Midori-ku, Yokohama 226-8501, Japan. Electronic address: hmihara@bio.titech.ac.jp.
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Mihara
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D000327:Adsorption / Q000187:drug effects; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D016501:Neurites / Q000187:drug effects; D016716:PC12 Cells; D010455:Peptides / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D051381:Rats; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D010455:Peptides
keywords: Cellular engineering; Cellular uptake; Differentiation; Peptides