title: Non-enzymatic electrochemical detection of cholesterol using β-cyclodextrin functionalized graphene.
abstract: A non-enzymatic approach towards cholesterol detection is presented here, exploiting the electrochemical non-enzymatic route of sensing which has a distinct advantage over other conventional enzymatic processes. Chemically converted Graphene modified with β-CD, being hydrophilic, electroactive and high surface area material, provides a platform for the electrochemical detection of cholesterol using Methylene Blue as redox indicator. Methylene Blue (MB) forms an inclusion complex with Grp-β-CD and emerges as a cholesterol sensing matrix. MB molecule is replaced by cholesterol molecule and moves out in the buffer solution, hence, detected electrochemically using Differential Pulse Voltammetric (DPV) technique. The sensing matrix is characterised using FT-IR and Raman spectroscopy. Transmission Electron Microscopy is carried out to study the morphology of functionalized graphene sheets.
authors.affiliation: Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF, Bidhannagar, Kolkata, West Bengal 700064, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Agnihotri
authors.affiliation: Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF, Bidhannagar, Kolkata, West Bengal 700064, India.
authors.forename: Ankan Dutta
authors.initials: AD
authors.lastname: Chowdhury
authors.affiliation: Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF, Bidhannagar, Kolkata, West Bengal 700064, India. Electronic address: amitabha.de@saha.ac.in.
authors.forename: Amitabha
authors.initials: A
authors.lastname: De
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002784:Cholesterol / Q000032:analysis* / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004798:Enzymes / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004798:Enzymes; D047392:beta-Cyclodextrins; D006108:Graphite; D002784:Cholesterol; C031215:betadex
keywords: Cholesterol sensing; Cyclic Voltammetery; Differential Pulse Voltammetery; Graphene-β-Cyclodextrin; Inclusion complex; Redox indicator