title: Analysis of interleukin-8 gene variants reveals their relative importance as genetic susceptibility factors for chronic periodontitis in the Han population.
abstract: Interleukin (IL)-8, an important chemokine that regulates the inflammatory response, plays an important role in periodontitis. Previous studies indicate that certain IL-8 gene polymorphisms are associated with periodontitis susceptibility in some populations. However, the literature is somewhat contradictory, and not all IL-8 polymorphisms have been examined, particularly in Han Chinese individuals. The aim of this study was to investigate the association of every IL-8 SNP with chronic periodontitis in Han Chinese individuals. We analyzed 23 SNPs with minor allele frequency (MAF)≥0.01, which were selected from 219 SNPs in the NCBI dbSNP and preliminary HapMap data analyses from a cohort of 400 cases and 750 controls from genetically independent Han Chinese individuals. Single SNP, haplotype and gender-specific associations were performed. We found that rs4073 and rs2227307 were significantly associated with chronic periodontitis. Further haplotype analysis indicated that a haplotype block (rs4073-rs2227307-rs2227306) that spans the promoter and exon1 of IL-8 was highly associated with chronic periodontitis. Additionally, the ATC haplotype in this block was increased 1.5-fold in these cases. However, when analyzing the samples by gender, no significant gender-specific associations in IL-8 were observed, similar to the results of haplotype association analyses in female and male subgroups. Our results provide further evidence that IL-8 is associated with chronic periodontitis in Han Chinese individuals. Furthermore, our results confirm previous reports suggesting the intriguing possibilities that IL-8 plays a role in the pathogenesis of chronic periodontitis and that this gene may be involved in the etiology of this condition.
authors.affiliation: Department of Dentistry, the First Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environment and Genes Related to Diseases, College of Medicine, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Psychiatry, Washington University in Saint Louis, Saint Louis, Missouri, United States of America.
authors.forename: Tianxiao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: The Second Department of Orthopedics, the Second Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Haojie
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of National Ministry of Health for Forensic Sciences, College of Medicine, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of National Ministry of Health for Forensic Sciences, College of Medicine, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Zufei
authors.initials: Z
authors.lastname: Feng
authors.affiliation: School of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Dexing
authors.initials: D
authors.lastname: Zhong
mesh_terms: D000328:Adult; D044466:Asian People / Q000208:ethnology; D016022:Case-Control Studies; D002681:China / Q000208:ethnology; D055113:Chronic Periodontitis / Q000208:ethnology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8
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