title: [Study on HPLC fingerprint of Eriobotrya japonica flower].
abstract: OBJECTIVE
  To establish an HPLC fingerprint of Eriobotrya japonica flower.
  
  
  METHODS
  13 batches of Eriobotrya japonica flowers from different habitats were analyzed by HPLC with ursolic acid as reference substance. The chromatographic condition was performed on Alltech Apollo C18 column (250 mm x 4.6 mm, 5 microm), eluted with mobile phase containing methanol and 0.1% glacial acetic acid in gradient mode. The flow rate was 0.5 mL/min and the detection wavelength was set at 210 nm. The temperature of column was 20 degrees C.
  
  
  RESULTS
  The HPLC fingerprint of Eriobotrya japonica flower had been established. There were 17 common peaks, two of which were identified by reference substances. 13 batches of sample from different habitats can be distinguished from their fingerprints.
  
  
  CONCLUSIONS
  This method is reasonable and reliable, which can be used for quality evaluation of Eriobotrya japonica flower.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Li
authors.forename: Bi-Kun
authors.initials: BK
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D031983:Eriobotrya / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D014315:Triterpenes / Q000737:chemistry; D000097245:Ursolic Acid
chemical_list: D004365:Drugs, Chinese Herbal; D014315:Triterpenes; D000097245:Ursolic Acid