title: [In vitro activity of ceftaroline against Spanish isolates of Staphylococcus aureus: a multicenter study].
abstract: BACKGROUND
  Ceftaroline fosamil is a new-generation antimicrobial agent of cephalosporins subgroup. It is the first commercially available beta-lactam antibiotic that exhibits activity against methicillin-resistant Staphylococcus aureus (MRSA). The aim of this study is to determine the in vitro Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values of ceftaroline against S.aureus strains (including MRSA).
  
  
  METHODS
  A multicenter study involving four hospitals representative of the Spanish geography was performed. MIC and MBC values against both the methicillin-resistant and sensitive strains of S.aureus (MRSA and methicillin-sensitive S.aureus [MSSA]) were determined using a broth microdilution method.
  
  
  RESULTS
  A total of 266 S.aureus strains were analyzed (95 MRSA and 171 MSSA). Ceftaroline bacterial sensitivity showed a mean MIC of 0.227 μg/ml (SD=0.146; range, 0.06 to 1 μg/ml). All MIC values of the 266 strains tested belonged to the sensitive category (value ≤ 1 μg/ml). Intermediate or resistant strains were not detected. MIC50 and MIC90 values for MRSA were 0.25 and 0.5 μg/ml, respectively (range=0.125-1 μg/ml). MSSA strains showed MIC50 and MIC90 values of 0.125 and 0.25 μg/ml, respectively (range=0.125-0.5 μg/ml). MBC50 and MBC90 values for MRSA were 0.5 and 1 μg/ml, respectively (range=0.125-1 μg/ml). MSSA strains showed MBC50 and MBC90 values of 0.25 and 0.25 μg/ml, respectively (range=0.125-0.5 μg/ml).
  
  
  CONCLUSIONS
  Ceftaroline shows excellent in vitro activity against S.aureus, including MRSA strains. Therefore, this antibiotic may be a promising alternative for the treatment of infections caused by this bacterium.
authors.affiliation: Unidad de Gestión Clínica de Microbiología, Complejo Hospitalario de Huelva, Huelva, España. Electronic address: albeteno@hotmail.com.
authors.forename: Alberto
authors.initials: A
authors.lastname: Tenorio-Abreu
authors.affiliation: Servicio de Microbiología, Hospital Universitario de la Ribera, Alcira, Valencia, España.
authors.forename: Jesús
authors.initials: J
authors.lastname: Gil Tomás
authors.affiliation: Servicio de Microbiología, Hospital Clínico Universitario de Valladolid, Valladolid, España.
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Bratos Pérez
authors.affiliation: Unidad de Gestión Clínica de Microbiología, Complejo Hospitalario de Huelva, Huelva, España.
authors.forename: Alberto
authors.initials: A
authors.lastname: de la Iglesia Salgado
authors.affiliation: Servicio de Microbiología, Hospital Universitario de la Ribera, Alcira, Valencia, España.
authors.forename: María
authors.initials: M
authors.lastname: Borrás Máñez
authors.affiliation: Servicio de Microbiología, Hospital Clínico Universitario de Valladolid, Valladolid, España.
authors.forename: Raúl
authors.initials: R
authors.lastname: Ortiz de Lejarazu Leonardo
authors.affiliation: Servicio de Microbiología, Hospital Clínico Universitario de Valladolid, Valladolid, España.
authors.forename: Ana
authors.initials: A
authors.lastname: Ávila Alonso
authors.affiliation: Servicio de Microbiología, Hospital Universitario de la Ribera, Alcira, Valencia, España.
authors.forename: Javier
authors.initials: J
authors.lastname: Colomina Rodríguez
authors.affiliation: Unidad de Gestión Clínica de Microbiología, Complejo Hospitalario de Huelva, Huelva, España.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Pérez Cáceres
authors.affiliation: Unidad de Gestión Clínica de Microbiología, Complejo Hospitalario de Huelva, Huelva, España.
authors.forename: José María
authors.initials: JM
authors.lastname: Saavedra Martín
authors.affiliation: Unidad de Gestión Clínica de Microbiología, Complejo Hospitalario de Huelva, Huelva, España.
authors.forename: Adriana
authors.initials: A
authors.lastname: Márquez Sanabria
authors.affiliation: Unidad de Gestión Clínica de Microbiología, Complejo Hospitalario de Huelva, Huelva, España.
authors.forename: Ana
authors.initials: A
authors.lastname: Domínguez Castaño
authors.affiliation: Unidad de Gestión Clínica de Microbiología, Complejo Hospitalario de Huelva, Huelva, España.
authors.forename: Matilde
authors.initials: M
authors.lastname: de la Iglesia Salgado
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002511:Cephalosporins / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D055815:Young Adult; D000097583:Ceftaroline
chemical_list: D002511:Cephalosporins; D000097583:Ceftaroline
keywords: Ceftarolina; Ceftaroline; Concentración mínima bactericida; Concentración mínima inhibitoria; Minimum bactericidal concentration; Minimum inhibitory concentration; Staphylococcus aureus