title: Nuclear factor Y-mediated H3K27me3 demethylation of the SOC1 locus orchestrates flowering responses of Arabidopsis.
abstract: Nuclear factor Y (NF-Y) is a conserved heterotrimeric transcription factor complex that binds to the CCAAT motifs within the promoter region of many genes. In plants, a large number of genes code for variants of each NF-YA, B or C subunit that can assemble in a combinatorial fashion. Here, we report the discovery of an Arabidopsis NF-Y complex that exerts epigenetic control over flowering time by integrating environmental and developmental signals. We show that NF-Y interacts with CONSTANS in the photoperiod pathway and DELLAs in the gibberellin pathway, to directly regulate the transcription of SOC1, a major floral pathway integrator. This NF-Y complex binds to a unique cis-element within the SOC1 promoter to modulate trimethylated H3K27 levels, partly through a H3K27 demethylase REF6. Our findings establish NF-Y complexes as critical mediators of epigenetic marks that regulate the response to environmental or intrinsic signals in plants.
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China.
authors.forename: Xingliang
authors.initials: X
authors.lastname: Hou
authors.affiliation: Department of Biological Sciences and Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Jiannan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Biological Sciences and Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Biological Sciences and Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Biological Sciences and Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Lisha
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Biological Sciences and Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
mesh_terms: D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D023081:CCAAT-Binding Factor / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000378:metabolism; D044127:Epigenesis, Genetic; D035264:Flowers / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008745:Methylation; D008969:Molecular Sequence Data; D017440:Photoperiod; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: C419046:AGL20 protein, Arabidopsis; D029681:Arabidopsis Proteins; D023081:CCAAT-Binding Factor; C092776:CONSTANS protein, Arabidopsis; D004268:DNA-Binding Proteins; C109555:GAI protein, Arabidopsis; D005875:Gibberellins; D006657:Histones; D026161:MADS Domain Proteins; C529524:REF6 protein, Arabidopsis; D014157:Transcription Factors; C402244:nuclear factor Y; D005982:Glutathione Transferase