title: Structural damage of chicken red blood cells exposed to platinum nanoparticles and cisplatin.
abstract: Side effects and resistance of cancer cells to cisplatin are major drawbacks to its application, and recently, the possibility of replacing cisplatin with nanocompounds has been considered. Most chemotherapeutic agents are administered intravenously, and comparisons between the interactions of platinum nanoparticles (NP-Pt) and cisplatin with blood compartments are important for future applications. This study investigated structural damage, cell membrane deformation and haemolysis of chicken embryo red blood cells (RBC) after treatment with cisplatin and NP-Pt. Cisplatin (4 μg/ml) and NP-Pt (2,6 μg/ml), when incubated with chicken embryo RBC, were detrimental to cell structure and induced haemolysis. The level of haemolytic injury was increased after cisplatin and NP-Pt treatments compared to the control group. Treatment with cisplatin caused structural damage to cell membranes and the appearance of keratocytes, while NP-Pt caused cell membrane deformations (discoid shape of cells was lost) and the formation of knizocytes and echinocytes. This work demonstrated that NP-Pt have potential applications in anticancer therapy, but potential toxic side effects must be explored in future preclinical research.
authors.affiliation: Division of Biotechnology and Biochemistry of Nutrition, Faculty of Animal Science, Warsaw University of Life Science, Warsaw 02-786, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kutwin
authors.affiliation: Division of Biotechnology and Biochemistry of Nutrition, Faculty of Animal Science, Warsaw University of Life Science, Warsaw 02-786, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Sawosz
authors.affiliation: Division of Biotechnology and Biochemistry of Nutrition, Faculty of Animal Science, Warsaw University of Life Science, Warsaw 02-786, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Jaworski
authors.affiliation: Division of Biotechnology and Biochemistry of Nutrition, Faculty of Animal Science, Warsaw University of Life Science, Warsaw 02-786, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Kurantowicz
authors.affiliation: Division of Biotechnology and Biochemistry of Nutrition, Faculty of Animal Science, Warsaw University of Life Science, Warsaw 02-786, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Strojny
authors.affiliation: Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3, Frederiksberg, Copenhagen 1870, Denmark.
authors.forename: André
authors.initials: A
authors.lastname: Chwalibog
keywords: Cancer therapy; Cisplatin; Haemolysis; Platinum nanoparticles; Red blood cells
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