title: Methane production and microbial community structure for alkaline pretreated waste activated sludge.
abstract: Alkaline pretreatment was studied to analyze the influence on waste activated sludge (WAS) reduction, methane production and microbial community structure during anaerobic digestion. Methane production from alkaline pretreated sludge (A-WAS) (pH = 12) increased from 251.2 mL/Ld to 362.2 mL/Ld with the methane content of 68.7% compared to raw sludge (R-WAS). Sludge reduction had been improved, and volatile suspended solids (VSS) removal rate and protein reduction had increased by ∼ 10% and ∼ 35%, respectively. The bacterial and methanogenic communities were analyzed using 454 pyrosequencing and clone libraries of 16S rRNA gene. Remarkable shifts were observed in microbial community structures after alkaline pretreatment, especially for Archaea. The dominant methanogenic population changed from Methanosaeta for R-WAS to Methanosarcina for A-WAS. In addition to the enhancement of solubilization and hydrolysis of anaerobic digestion of WAS, alkaline pretreatment showed significant impacts on the enrichment and syntrophic interactions between microbial communities.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: dxing@hit.edu.cn.
authors.forename: Defeng
authors.initials: D
authors.lastname: Xing
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Jianna
authors.initials: J
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Aijuan
authors.initials: A
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: rnq@hit.edu.cn.
authors.forename: Nanqi
authors.initials: N
authors.lastname: Ren
mesh_terms: D001105:Archaea / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D001426:Bacterial Proteins / Q000302:isolation & purification; D057919:Biological Oxygen Demand Analysis; D005232:Fatty Acids, Volatile / Q000096:biosynthesis; D008697:Methane / Q000096:biosynthesis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D012972:Sodium Hydroxide / Q000494:pharmacology*; D014835:Volatilization; D014866:Waste Products
chemical_list: D001426:Bacterial Proteins; D005232:Fatty Acids, Volatile; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014866:Waste Products; D012972:Sodium Hydroxide; D008697:Methane
keywords: 454 pyrosequencing; Alkaline pretreatment; Methane production; Microbial community; Waste activated sludge (WAS)