title: PI3K, MAPK EPK1/2 and p38 are involved in the realization of growth potential of mesenchymal progenitor cells under the influence of basic fibroblast growth factor.
abstract: We studied PI3K, MAPK/EPK1/2 and p38-dependent signaling cascade under stimulation of the functions of mesenchymal progenitor cells by fibroblast growth factor. The important role of PI3K and EPK1/2 in the realization of their growth potential has been revealed. Experiments have demonstrated a blocking effect of PI3K inhibitor on proliferation and differentiation of progenitor cells and EPK1/2 inhibitor on mitotic activity of the progenitor cells. The p38-mediated MAPK pathway did not play a role in determining the proliferative and differentiation status of fibroblast CFU.
authors.affiliation: Research Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, Russia, zgn@pharm.tsu.ru.
authors.forename: G N
authors.initials: GN
authors.lastname: Zyuz'kov
authors.forename: M G
authors.initials: MG
authors.lastname: Danilets
authors.forename: A A
authors.initials: AA
authors.lastname: Ligacheva
authors.forename: V V
authors.initials: VV
authors.lastname: Zhdanov
authors.forename: E V
authors.initials: EV
authors.lastname: Udut
authors.forename: L A
authors.initials: LA
authors.lastname: Miroshnichenko
authors.forename: E V
authors.initials: EV
authors.lastname: Simanina
authors.forename: A V
authors.initials: AV
authors.lastname: Chaikovskii
authors.forename: E S
authors.initials: ES
authors.lastname: Trofimova
authors.forename: M Yu
authors.initials: MY
authors.lastname: Minakova
authors.forename: V V
authors.initials: VV
authors.lastname: Udut
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005786:Gene Expression Regulation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D061251:Primary Cell Culture; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D016222:Fibroblast Growth Factor 2; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors; D048051:p38 Mitogen-Activated Protein Kinases