title: A diagnosis of giant cell-rich tumour of bone is supported by p63 immunohistochemistry, when more than 50 % of cells is stained.
abstract: Some primary malignant or benign tumours of bone contain numerous multinucleated cells. These "giant cell-rich tumours of bone" have overlapping features and clinical and radiological data are needed to reach an accurate pathological diagnosis. We studied the potential contribution of p63 immunohistochemistry to the reliability of the histological diagnosis. We performed a multicentric retrospective study of 291 giant cell-rich tumours of bone which included 119 giant cell tumours of bone (GCTB), 76 aneurysmal bone cysts (ABC), 49 chondroblastomas (CB), 15 nonossifying fibromas (NOF), 10 giant cell reparative granulomas (RG) of jaws, 1 giant cell lesion of small bones, 2 hyperparathyroidism-related brown tumours (BT), 17 bone sarcomas with numerous osteoclasts and 2 malignant giant cell tumours of bone. p63 is expressed in ABC, CB, NOF, RG, BT and GCTB, but its expression in more than 50 % of mononuclear cells is strongly suggestive of a diagnosis of GCTB. In contrast, malignant GCTB were mostly negative. Our results show that p63 is expressed in a broad range of benign giant cell-rich tumours of bone, consistent with data in the recent literature, while infrequent in malignant tumours. With a cut-off 50 %, the presence of p63 positive cells is useful in supporting a diagnosis of giant cell-rich tumour of bone. However, a final diagnosis cannot be made without due consideration of all clinical/radiological and pathological data.
authors.affiliation: Service d'Anatomie Pathologique, Hôpital la Timone, UMR911 AMU, Marseille, France, Andre.MauesDePaula@ap-hm.fr.
authors.forename: André
authors.initials: A
authors.lastname: Maues De Paula
authors.forename: Alexandre
authors.initials: A
authors.lastname: Vasiljevic
authors.forename: Roch
authors.initials: R
authors.lastname: Giorgi
authors.forename: Anne
authors.initials: A
authors.lastname: Gomez-Brouchet
authors.forename: Sébastien
authors.initials: S
authors.lastname: Aubert
authors.forename: Xavier
authors.initials: X
authors.lastname: Leroy
authors.forename: Hélène
authors.initials: H
authors.lastname: Duval
authors.forename: Gonzague
authors.initials: G
authors.lastname: de Pinieux
authors.forename: Corinne
authors.initials: C
authors.lastname: Bouvier
mesh_terms: D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000032:analysis*; D001859:Bone Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D018212:Giant Cell Tumor of Bone / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D012372:ROC Curve; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor; C081876:CKAP4 protein, human; D008565:Membrane Proteins
references.citation: Hum Pathol. 1985 Mar;16(3):287-93
references.pmid: 2579018
references.citation: Tumour Biol. 2012 Oct;33(5):1639-44
references.pmid: 22623071
references.citation: Arch Pathol Lab Med. 2011 Jun;135(6):776-9
references.pmid: 21631272
references.citation: Virchows Arch. 2012 May;460(5):525-34
references.pmid: 22543453
references.citation: Virchows Arch. 2010 Jan;456(1):97-103
references.pmid: 20012988
references.citation: Am J Pathol. 2004 Nov;165(5):1773-80
references.pmid: 15509545
references.citation: Hum Pathol. 2010 Feb;41(2):208-13
references.pmid: 19801163
references.citation: Ann Diagn Pathol. 2002 Jun;6(3):159-63
references.pmid: 12089726
references.citation: Carcinogenesis. 2004 Jun;25(6):857-64
references.pmid: 15033906
references.citation: Diagn Pathol. 2012 Sep 27;7:130
references.pmid: 23016917
references.citation: Cancer Res. 2004 Mar 15;64(6):1920-3
references.pmid: 15026324
references.citation: Oncogene. 2005 May 12;24(21):3419-26
references.pmid: 15735689
references.citation: Mod Pathol. 2008 Apr;21(4):369-75
references.pmid: 18311114
references.citation: Tumori. 2004 Mar-Apr;90(2):239-43
references.pmid: 15237589
references.citation: Mod Pathol. 2008 May;21(5):531-9
references.pmid: 18192965
references.citation: Int J Surg Pathol. 2013 Feb;21(1):48-53
references.pmid: 22736299
references.citation: Skeletal Radiol. 2003 Mar;32(3):143-6
references.pmid: 12605278
references.citation: Int J Oncol. 2013 Feb;42(2):437-43
references.pmid: 23229819
references.citation: Ups J Med Sci. 2013 Mar;118(1):23-8
references.pmid: 23033898
references.citation: Arch Pathol Lab Med. 2008 Sep;132(9):1388-96
references.pmid: 18788849
references.citation: Genes Chromosomes Cancer. 1999 Nov;26(3):265-6
references.pmid: 10502326
references.citation: Clin Cancer Res. 2002 Feb;8(2):494-501
references.pmid: 11839669
references.citation: Int Orthop. 2006 Dec;30(6):484-9
references.pmid: 17013643