title: Murine xenograft model demonstrates significant radio-sensitising effect of liposomal doxorubicin in a combination therapy for Feline Injection Site Sarcoma.
abstract: Therapy of cats suffering from feline injection site sarcomas (FISS) is still a challenging problem, as the recurrence rate after surgery is up to 70%. Four FISS-derived primary tumour cell lines and corresponding xenograft tumour mouse models were established to evaluate the efficacy of a concomitant chemo-/radiation therapy with doxorubicin. In vitro, strongly depending upon the timing of administration, pre-treatment with 0.25 µmol doxorubicin resulted in a significant enhancement of radiation-induced (3.5 Gy) tumour cell death. This result was confirmed in vivo, where only the combined chemo-/radiation therapy resulted in a significant reduction in tumour growth compared to the respective mono-therapies with either doxorubicin or radiation. These results support the use of the concomitant chemo-/radiation therapy for adjuvant treatment of FISS, particularly in advanced or recurrent disease where surgery alone is no longer feasible.
authors.affiliation: Institute of Virology, Department for Pathobiology, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria. Electronic address: Helga.Petznek@vetmeduni.ac.at.
authors.forename: H
authors.initials: H
authors.lastname: Petznek
authors.affiliation: Radiooncology and Nuclear Medicine Platform, Department for Companion Animals and Horses, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Kleiter
authors.affiliation: Bioinformatics and Biostatistics Platform, Department for Biomedical Sciences, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Tichy
authors.affiliation: Institute of Pathology and Forensic Veterinary Medicine, Department for Pathobiology, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Fuchs-Baumgartinger
authors.affiliation: Institute of Virology, Department for Pathobiology, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: C
authors.initials: C
authors.lastname: Hohenadl
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use*; D002371:Cat Diseases / Q000628:therapy*; D002415:Cats; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009372:Neoplasms, Connective Tissue / Q000628:therapy / Q000662:veterinary*; D011092:Polyethylene Glycols / Q000627:therapeutic use; D011878:Radiotherapy / Q000379:methods / Q000662:veterinary*; D012509:Sarcoma / Q000628:therapy / Q000662:veterinary*; D013997:Time Factors; D014183:Transplantation, Heterologous / Q000379:methods / Q000662:veterinary; D016896:Treatment Outcome; D023041:Xenograft Model Antitumor Assays / Q000379:methods / Q000662:veterinary*
chemical_list: D000903:Antibiotics, Antineoplastic; C506643:liposomal doxorubicin; D011092:Polyethylene Glycols; D004317:Doxorubicin
keywords: Doxorubicin; FISS; Primary feline sarcoma cell culture; Radiation; Xenograft tumour mouse model