title: MicroRNA-665 is involved in the regulation of the expression of the cardioprotective cannabinoid receptor CB2 in patients with severe heart failure.
abstract: The myocardial endocannabinoid system has been linked to stress response and cardioprotection. In chronic heart failure (CHF), protective CB2 receptors are markedly up-regulated while CB1 receptors are slightly down-regulated. We here provide evidence that myocardial CB receptors are subject to microRNA regulation. By a combined computational and experimental approach we show that CB1 receptors are regulated by miR-494, and CB2 receptors are targeted by miR-665. Moreover, we demonstrate that in CHF, miR-665 expression is significantly decreased while miR-494 is slightly increased, which is concordant with the previously reported alterations of CB receptors. These results suggest that in CHF, altered expression of specific miRNAs may contribute to a compensatory response of the diseased myocardium.
authors.affiliation: Research Group Molecular Medicine, Department of Anesthesiology, University of Munich (LMU), Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Möhnle
authors.affiliation: Research Group Molecular Medicine, Department of Anesthesiology, University of Munich (LMU), Germany.
authors.forename: Stefanie Veronika
authors.initials: SV
authors.lastname: Schütz
authors.affiliation: Research Group Molecular Medicine, Department of Anesthesiology, University of Munich (LMU), Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Schmidt
authors.affiliation: Research Group Molecular Medicine, Department of Anesthesiology, University of Munich (LMU), Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Hinske
authors.affiliation: Research Group Molecular Medicine, Department of Anesthesiology, University of Munich (LMU), Germany.
authors.forename: Max
authors.initials: M
authors.lastname: Hübner
authors.affiliation: Research Group Molecular Medicine, Department of Anesthesiology, University of Munich (LMU), Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Heyn
authors.affiliation: Department of Cardiac Surgery, Goethe University Frankfurt, Frankfurt, Germany.
authors.forename: Andres
authors.initials: A
authors.lastname: Beiras-Fernandez
authors.affiliation: Research Group Molecular Medicine, Department of Anesthesiology, University of Munich (LMU), Germany. Electronic address: simone.kreth@med.uni-muenchen.de.
authors.forename: Simone
authors.initials: S
authors.lastname: Kreth
mesh_terms: D006333:Heart Failure / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D043884:Receptor, Cannabinoid, CB1 / Q000096:biosynthesis*; D043885:Receptor, Cannabinoid, CB2 / Q000096:biosynthesis*
chemical_list: C547168:MIRN494 microRNA, human; C000592932:MIRN665 microRNA, human; D035683:MicroRNAs; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2
keywords: CB1; CB2; Endocannabinoid system; MicroRNA