title: The inflammation-related gene S100A12 is positively regulated by C/EBPβ and AP-1 in pigs.
abstract: S100A12 is involved in the inflammatory response and is considered an important marker for many inflammatory diseases in humans. Our previous studies indicated that the S100A12 gene was abundant in the immune tissues of pigs and was significantly upregulated during infection with Haemophilus parasuis (HPS) or porcine circovirus type 2 (PCV2). In this study, the mechanism of transcriptional regulation of S100A12 was investigated in pigs. Our results showed that S100A12, CCAAT/enhancer-binding protein beta (C/EBPβ) and activator protein-1 (AP-1) genes were up-regulated in PK-15 (ATCC, CCL-33) cells when treated with LPS or Poly I: C. Additionally, the promoter activity and expression level of the S100A12 gene were significantly upregulated when C/EBPβ or AP-1 were overexpressed. We utilized electromobility shift assays (EMSA) to confirm that C/EBPβ and AP-1 could directly bind the S100A12 gene promoter. We also found that the transcriptional activity and expression levels of C/EBPβ and AP-1 could positively regulate each other. Furthermore, the promoter activity of the S100A12 gene was higher when C/EBPβ and AP-1 were cotransfected than when they were transfected individually. We concluded that the S100A12 gene was cooperatively and positively regulated by C/EBPβ and AP-1 in pigs. Our study offers new insight into the transcriptional regulation of the S100A12 gene.
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. xyli@mail.hzau.edu.cn.
authors.forename: Xinyun
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. tangjuan1113@yeah.net.
authors.forename: Juan
authors.initials: J
authors.lastname: Tang
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. hzauxujing@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. zhumengjin@mail.hzau.edu.cn.
authors.forename: Mengjin
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. jhcao@mail.hzau.edu.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. Liuying_K4@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. yumei@mail.hzau.edu.cn.
authors.forename: Mei
authors.initials: M
authors.lastname: Yu
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. shzhao@mail.hzau.edu.cn.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D007249:Inflammation / Q000235:genetics / Q000473:pathology; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008969:Molecular Sequence Data; D011070:Poly I-C / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D009418:S100 Proteins / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D018808:Transcription Factor AP-1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D008070:Lipopolysaccharides; D009418:S100 Proteins; D018808:Transcription Factor AP-1; D048031:JNK Mitogen-Activated Protein Kinases; D011070:Poly I-C
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