title: Zinc supplementation protects against cadmium accumulation and cytotoxicity in Madin-Darby bovine kidney cells.
abstract: Cadmium ions (Cd2+) have been reported to accumulate in bovine tissues, although Cd2+ cytotoxicity has not been investigated thoroughly in this species. Zinc ions (Zn2+) have been shown to antagonize the toxic effects of heavy metals such as Cd2+ in some systems. The present study investigated Cd2+ cytotoxicity in Madin-Darby bovine kidney (MDBK) epithelial cells, and explored whether this was modified by Zn2+. Exposure to Cd2+ led to a dose- and time-dependent increase in apoptotic cell death, with increased intracellular levels of reactive oxygen species and mitochondrial damage. Zn2+ supplementation alleviated Cd2+-induced cytotoxicity and this protective effect was more obvious when cells were exposed to a lower concentration of Cd2+ (10 μM), as compared to 50 μM Cd2+. This indicated that high levels of Cd2+ accumulation might induce irreversible damage in bovine kidney cells. Metallothioneins (MTs) are metal-binding proteins that play an essential role in heavy metal ion detoxification. We found that co-exposure to Zn2+ and Cd2+ synergistically enhanced RNA and protein expression of MT-1, MT-2, and the metal-regulatory transcription factor 1 in MDBK cells. Notably, addition of Zn2+ reduced the amounts of cytosolic Cd2+ detected following MDBK exposure to 10 μM Cd2+. These findings revealed a protective role of Zn2+ in counteracting Cd2+ uptake and toxicity in MDBK cells, indicating that this approach may provide a means to protect livestock from excessive Cd2+ accumulation.
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Ding
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Jingying
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Shahzad
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Zhaoqing
authors.initials: Z
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Jiakui
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
authors.forename: Hong
authors.initials: H
authors.lastname: Sjölinder
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002104:Cadmium / Q000493:pharmacokinetics* / Q000633:toxicity*; D002417:Cattle; D002460:Cell Line; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism; D013997:Time Factors; D015032:Zinc / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D002104:Cadmium; D008668:Metallothionein; D015032:Zinc
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