title: Haplotype data of mitochondrial DNA coding region encompassing nucleotide positions 11,719-12,184 and evaluate the importance of these positions for forensic genetic purposes in Iraq.
abstract: Samples of 100 random healthy unrelated Iraqi male persons from the Arab ethnic group of Iraqi population were collected for mtDNA coding region sequencing by using the Sanger technique and to establish the degree of variation characteristic of a fragment. Portion of coding region encompassing positions 11,719-12,184 was amplified in accordance with the Anderson reference sequence. PCR products were purified by EZ-10 spin column then sequenced and detected by using the ABI 3130xL DNA Analyzer. This is to intend the detection of polymorphisms of mtDNA. Four new polymorphic positions 11,741, 11,756, 11,878, and 12,133 are described which may be suitable in the future to be the sources for human identification purpose in Iraq. The obtained data can be used to identify variable nucleotide positions characterized by frequent occurrence most promising for identification variants. The calculated value GD = 0.95 and RMP = 0.048 of the genetic diversity should be understood as high in the context of coding function of the analysed DNA fragment. The relatively high gene diversity and a relatively low random match probability were observed in this study.
authors.affiliation: a Department of Molecular Biology , Babylon University , Hilla City , Iraq .
authors.forename: Imad Hadi
authors.initials: IH
authors.lastname: Hameed
authors.affiliation: b Centre of DNA Research , Babylon University , Hilla City , Iraq .
authors.forename: Mohammed Abdulla
authors.initials: MA
authors.lastname: Jebor
authors.affiliation: c Institute of Medico-Legal in Baghdad, Ministry of Health of Iraq , Iraq , and.
authors.forename: Aamera Jaber
authors.initials: AJ
authors.lastname: Ommer
authors.affiliation: a Department of Molecular Biology , Babylon University , Hilla City , Iraq .
authors.forename: Ameer Ibrahim
authors.initials: AI
authors.lastname: Abdulzahra
authors.affiliation: d Department of Biomedical Science , University Putra Malaysia , Selangor , Malaysia.
authors.forename: Cheah
authors.initials: C
authors.lastname: Yoke
mesh_terms: D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods*; D006239:Haplotypes; D006801:Humans; D007493:Iraq; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020641:Polymorphism, Single Nucleotide
chemical_list: D004272:DNA, Mitochondrial
keywords: Coding region; Iraq; genetic diversity; haplotype; mitochondrial DNA; polymorphic positions; random match probability