title: Effect of sonication on different quality parameters of Pinus massoniana pollen.
abstract: A study was initiated with the objective of evaluating the effects of sonication treatment on important quality parameters of extract of Pinus massoniana pollen. Sonication of extract was done (frequency 20kHz and various amplitude levels) for 10, 30, 50min, respectively. As results, total polysaccharide, phenolics and flavonoids significantly increased (P<0.05). And sonicated P.massoniana pollen displays strong immuno-stimulating activity by increasing proliferations of splenic lymphocytes and subsets of CD4+ T cells (CD3+CD4+), CD8 T cells (CD3+CD8+), and increased Ig secretion. Sonicated P. massoniana pollen also showed anti-tumor function by inhibition of tumor cell proliferation, inhibition of ROS production, up-regulation of GSH/GSSG ration, up-regulating the gene expression of P53, Bax and down-regulating the gene expression of Bcl-2. Findings of the present study suggested the sonication treatment of P. massoniana pollen could improve the quality and bioactivity of P. massoniana pollen, indicating that sonication is effective in processing of pollen and could be a potential process in tumor prevention and treatment.
authors.affiliation: Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Fu-bo
authors.initials: FB
authors.lastname: Zhang
authors.affiliation: Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China. Electronic address: shanhu67@163.com.
authors.forename: Hu
authors.initials: H
authors.lastname: Shan
authors.affiliation: Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: puyanchen@yahoo.com.cn.
authors.forename: Pu-Yan
authors.initials: PY
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D010636:Phenols / Q000032:analysis; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011058:Pollen / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D011786:Quality Control; D013010:Sonication; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D005419:Flavonoids; D007136:Immunoglobulins; D010636:Phenols; D010936:Plant Extracts; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein
keywords: Anti-tumor; Immunomodulatory function; Pinus massoniana pollen; Sonication