title: Live imaging of cellular dynamics using a multi-imaging vector in single cells.
abstract: Real-time monitoring of cellular dynamics in living organisms is highly challenging. We developed a multi-imaging vector based on 2A peptides. Live imaging of subcellular compartments can be performed following the transfection of cells with another vector, the multi-labeling vector, which contains localization signals and various fluorescent protein variants.
authors.affiliation: BioNano Health Guard Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Republic of Korea. chungbh@kribb.re.kr.
authors.forename: Kyoungsook
authors.initials: K
authors.lastname: Park
authors.forename: Jinyoung
authors.initials: J
authors.lastname: Jeong
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D005822:Genetic Vectors / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012328:RNA Viruses / Q000235:genetics; D014162:Transfection
chemical_list: D010455:Peptides; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins