title: Role of AMPK α in skeletal muscle glycometabolism regulation and adaptation in relation to sepsis.
abstract: BACKGROUND
  AMP-activated protein kinase (AMPK) and the translocation of glucose transporter 4 (GLUT4) protein always involve disturbance of carbohydrate metabolism.
  
  
  OBJECTIVE
  To determine whether the change of blood glucose in the early stage of septic rat is associated with the alteration of AMPKα protein expression and GLUT4 protein translocation expression.
  
  
  METHODS
  Animal models of sepsis were induced by tail vein injection of LPS in Wistar rats. The dynamic values of blood glucose within 2 hours after injection of LPS were observed. AMPKα protein and GLUT4 protein translocation in different tissues (such as soleus muscle and extensor digitorum longus) were assessed by western blot.
  
  
  RESULTS
  Blood glucose levels appeared to rise at 0.5 h after injection of LPS, arrived the peak value at 1 h, then fell at 1.5 h and 2 h Animals in LPS group experienced the increase of phos-AMPKα protein and GLUT4 protein translocation expression in soleus muscle and extensor digitorum longus.
  
  
  CONCLUSIONS
  The dynamic change of blood glucose, represented in a form of initiative increase and subsequent decrease in the early stage of sepsis, may be related to glycometabolism disorder in the skeletal muscle, coming down to enhancement of GLUT4 translocation expression promoted by activation of AMPKα.
authors.affiliation: Intensive Care Unit, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Intensive Care Unit, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Xu
authors.affiliation: Intensive Care Unit, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D050260:Carbohydrate Metabolism / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation; D051381:Rats; D018805:Sepsis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction / Q000235:genetics
chemical_list: D051275:Glucose Transporter Type 4; D008070:Lipopolysaccharides; D055372:AMP-Activated Protein Kinases; C568305:Prkaa1 protein, rat; D005947:Glucose
references.citation: PLoS One. 2011;6(9):e23999
references.pmid: 21931634
references.citation: Food Chem Toxicol. 2012 Dec;50(12):4356-63
references.pmid: 22960630
references.citation: Med Clin North Am. 2012 Nov;96(6):1095-105
references.pmid: 23102479
references.citation: N Engl J Med. 2006 Feb 2;354(5):449-61
references.pmid: 16452557
references.citation: Trends Cell Biol. 2001 Apr;11(4):173-9
references.pmid: 11306298
references.citation: Adv Surg. 2013;47:287-97
references.pmid: 24298857
references.citation: Biochem Soc Trans. 2005 Apr;33(Pt 2):362-6
references.pmid: 15787607
references.citation: Intensive Care Med. 2005 Aug;31(8):1136
references.pmid: 16052274
references.citation: Am J Physiol Endocrinol Metab. 2007 Aug;293(2):E453-9
references.pmid: 17505052
references.citation: CJEM. 2006 Sep;8(5):339-43
references.pmid: 17338845
references.citation: Trends Biochem Sci. 2004 Jan;29(1):18-24
references.pmid: 14729328
references.citation: Cardiovasc J Afr. 2010 Mar-Apr;21(2):72-8
references.pmid: 20532430
references.citation: J Biol Chem. 1996 Nov 1;271(44):27879-87
references.pmid: 8910387
references.citation: J Diabetes Sci Technol. 2012 Jan 01;6(1):37-47
references.pmid: 22401321
references.citation: World J Surg. 1999 Jun;23(6):553-9
references.pmid: 10227923
references.citation: Cell Mol Biol (Noisy-le-grand). 2011 Jun 23;57 Suppl:OL1513-9
references.pmid: 21699766
references.citation: Rev Diabet Stud. 2009 Spring;6(1):13-36
references.pmid: 19557293
references.citation: J Biol Chem. 2003 Aug 1;278(31):28372-7
references.pmid: 12766162
references.citation: J Biomed Res. 2010 Sep;24(5):389-94
references.pmid: 23554654
references.citation: J Biol Chem. 2005 Nov 25;280(47):39033-41
references.pmid: 16186119
references.citation: J Appl Physiol (1985). 2000 Dec;89(6):2430-7
references.pmid: 11090599
references.citation: Am J Physiol. 1999 May;276(5):E938-44
references.pmid: 10329989
references.citation: J Orthop Trauma. 2013 Jan;27(1):16-21
references.pmid: 22588532
references.citation: JAMA. 2003 Oct 15;290(15):2041-7
references.pmid: 14559958
references.citation: Am J Respir Crit Care Med. 2003 Feb 15;167(4):485-6
references.pmid: 12588709
references.citation: Mayo Clin Proc. 2003 Dec;78(12):1471-8
references.pmid: 14661676
references.citation: Biosci Rep. 2009 Jun 15;29(5):283-92
references.pmid: 18842111
references.citation: Shock. 2013 Jan;39(1):104-13
references.pmid: 23247127
references.citation: N Engl J Med. 2001 Nov 8;345(19):1359-67
references.pmid: 11794168
references.citation: Acupunct Med. 2011 Dec;29(4):276-83
references.pmid: 21697213
references.citation: Thyroid. 2012 Jan;22(1):70-9
references.pmid: 22136156
references.citation: Diabetologia. 2005 Sep;48(9):1798-809
references.pmid: 16052330
references.citation: Nurs Manage. 2009 Dec;40(12):42-5
references.pmid: 19966553
references.citation: Diabetes Metab. 2001 Sep;27(4 Pt 1):415-23
references.pmid: 11547215
references.citation: Am J Physiol. 1996 Apr;270(4 Pt 1):E667-76
references.pmid: 8928775
references.citation: Am J Physiol Endocrinol Metab. 2005 Jun;288(6):E1062-6
references.pmid: 15657088
references.citation: Mayo Clin Proc. 2004 Aug;79(8):992-1000
references.pmid: 15301325
references.citation: Mol Cell Biochem. 1999 Jun;196(1-2):79-83
references.pmid: 10448905
references.citation: Curr Biol. 2000 Oct 19;10(20):1247-55
references.pmid: 11069105
references.citation: Biochem Soc Trans. 2003 Dec;31(Pt 6):1290-4
references.pmid: 14641045
references.citation: Food Funct. 2013 Nov;4(11):1639-46
references.pmid: 24061149
references.citation: Cell Metab. 2007 Apr;5(4):237-52
references.pmid: 17403369
references.citation: J Anim Sci. 2011 Dec;89(12):4286-93
references.pmid: 21788428
references.citation: Arch Med Res. 2005 Nov-Dec;36(6):646-59
references.pmid: 16216646
references.citation: Diabetes. 2007 Mar;56(3):836-48
references.pmid: 17327455
references.citation: J Appl Physiol (1985). 2000 Mar;88(3):1072-5
references.pmid: 10710405
references.citation: Circ Res. 1996 May;78(5):839-47
references.pmid: 8620604
references.citation: PLoS One. 2012;7(2):e32520
references.pmid: 22389706
references.citation: Biochem J. 2000 Feb 1;345 Pt 3:437-43
references.pmid: 10642499
references.citation: Biosci Rep. 2012 Nov 30;33(1):37-47
references.pmid: 23050932