title: Human borna disease virus infection impacts host proteome and histone lysine acetylation in human oligodendroglia cells.
abstract: BACKGROUND
  Borna disease virus (BDV) replicates in the nucleus and establishes persistent infections in mammalian hosts. A human BDV strain was used to address the first time, how BDV infection impacts the proteome and histone lysine acetylation (Kac) of human oligodendroglial (OL) cells, thus allowing a better understanding of infection-driven pathophysiology in vitro.
  
  
  METHODS
  Proteome and histone lysine acetylation were profiled through stable isotope labeling for cell culture (SILAC)-based quantitative proteomics. The quantifiable proteome was annotated using bioinformatics. Histone acetylation changes were validated by biochemistry assays.
  
  
  RESULTS
  Post BDV infection, 4383 quantifiable differential proteins were identified and functionally annotated to metabolism pathways, immune response, DNA replication, DNA repair, and transcriptional regulation. Sixteen of the thirty identified Kac sites in core histones presented altered acetylation levels post infection.
  
  
  CONCLUSIONS
  BDV infection using a human strain impacted the whole proteome and histone lysine acetylation in OL cells.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Neurology, The Fifth People's Hospital of Shanghai, School of Medicine, Fudan University, Shanghai, 200240, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Neurology, The Third People's Hospital of Chongqing, 400014, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Bornavirus Research Group affiliated to the Free University of Berlin, Berlin, Germany.
authors.forename: Liv
authors.initials: L
authors.lastname: Bode
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Chengyu
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Rehabilitative Medicine, The Second Afﬁliated Hospital of Chongqing Medical University, Chongqing, 400010, China.
authors.forename: Rongzhong
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Lujun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Siwen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Jingjie PTM BioLab (Hangzhou) Co. Ltd, Hangzhou, 310018, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Jingjie PTM BioLab (Hangzhou) Co. Ltd, Hangzhou, 310018, China.
authors.forename: Tieming
authors.initials: T
authors.lastname: He
authors.affiliation: Advanced Institute of Translational Medicine, Tongji University, Shanghai, 200092, China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, 400016, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China. Electronic address: xiepeng@cqmu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
mesh_terms: D000107:Acetylation; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001890:Borna Disease / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D001891:Borna disease virus / Q000502:physiology*; D005260:Female; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000378:metabolism* / Q000821:virology; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics
chemical_list: D006657:Histones; D020543:Proteome; D008239:Lysine
keywords: Bioinformatics-assisted analysis; Borna disease virus; Histone; Human BDV; Lysine acetylation; Oligodendroglia cells; Proteomic
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