title: Irreversible magnetoporation of micro-organisms in high pulsed magnetic fields.
abstract: Electroporation is an appealing way of stimulating living cells, which causes permanent or temporary nanoporosities in the structure of the biological objects. However, the technique has a disadvantage such as a requirement of contact between the electrodes and the cell medium. In this review, a methodology of contactless treatment of the biological objects using pulsed magnetic fields is proposed. The eukaryotic micro-organisms Achlya americana and Saprolegnia diclina have been used in the study and magnetic fields up to 7 T were applied, which caused effects similar to irreversible electroporation resulting in the death of the species. The proposed technique is applicable for different types of the biological cells or micro-organisms and possibly can be used in the area of cancer, antifungal treatment and other biotechnological fields.
authors.affiliation: High Magnetic Field Laboratory, Vilnius Gediminas Technical University, Naugarduko g. 41, Vilnius 03227, Lithuania.
authors.forename: Vitalij
authors.initials: V
authors.lastname: Novickij
authors.affiliation: High Magnetic Field Laboratory, Vilnius Gediminas Technical University, Naugarduko g. 41, Vilnius 03227, Lithuania. audrius.grainys@vgtu.lt.
authors.forename: Audrius
authors.initials: A
authors.lastname: Grainys
authors.affiliation: High Magnetic Field Laboratory, Vilnius Gediminas Technical University, Naugarduko g. 41, Vilnius 03227, Lithuania.
authors.forename: Jurij
authors.initials: J
authors.lastname: Novickij
authors.affiliation: Laboratory of Mycology, Institute of Botany of Nature Research Centre, Zaliuju ezeru g. 49, Vilnius 08406, Lithuania.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Markovskaja
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002458:Cell Fractionation / Q000379:methods*; D002463:Cell Membrane Permeability / Q000502:physiology* / Q000528:radiation effects*; D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D018274:Electroporation / Q000379:methods*; D060526:Magnetic Fields; D058608:Nanopores / Q000648:ultrastructure; D011829:Radiation Dosage; D044762:Saprolegnia / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D013045:Species Specificity