title: D-cycloserine 24 and 48 hours after asphyxial cardiac arrest has no effect on hippocampal CA1 neuropathology.
abstract: Stimulation of N-methyl-D-aspartate receptors (NMDAR) contributes to regenerative neuroplasticity following the initial excitotoxic insult during cerebral ischemia. Stimulation of NMDAR with the partial NMDAR agonist D-cycloserine (DCS) improves outcome and restores hippocampal synaptic plasticity in models of closed head injury. We thus hypothesized that DCS would improve outcome following restoration of spontaneous circulation (ROSC) from cardiac arrest (CA). DCS (10 mg/kg, IP) was administered to Sprague-Dawley rats (male, 250-330 g; 63-84 days old) 24 and 48 hours after 6 or 8 minutes of asphyxial CA. Heart rate and blood pressure declined similarly in all groups. Animals showed neurological deficits after 6 and 8 minutes CA (P<0.05, Tukey) and these deficits recovered more quickly after 6 minutes than after 8 minutes of CA. CA decreased the number of healthy neurons within CA1 with no difference between 6 and 8 minutes duration of CA (180.8±27.6 (naïve, n=5) versus 46.3±33.8 (all CA groups, n=27) neurons per mm CA1). DCS had no effect on neurological deficits or CA1 hippocampal cell counts (P>0.05, Tukey).
authors.affiliation: 1] Alaska Neuroscience Program, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska, USA [2] Department of Chemistry and Biochemistry, University of Alaska Fairbanks, Fairbanks, Alaska, USA.
authors.forename: Vélvá M
authors.initials: VM
authors.lastname: Combs
authors.affiliation: Alaska Neuroscience Program, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska, USA.
authors.forename: Heather D
authors.initials: HD
authors.lastname: Crispell
authors.affiliation: 1] Alaska Neuroscience Program, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska, USA [2] Department of Chemistry and Biochemistry, University of Alaska Fairbanks, Fairbanks, Alaska, USA.
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Drew
mesh_terms: D000818:Animals; D001237:Asphyxia / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D003523:Cycloserine / Q000494:pharmacology / Q000627:therapeutic use*; D006323:Heart Arrest / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D006339:Heart Rate / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D003523:Cycloserine
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