title: Interferon-γ induces immunoproteasomes and the presentation of MHC I-associated peptides on human salivary gland cells.
abstract: A prominent histopathological feature of Sjögren's syndrome, an autoimmune disease, is the presence of lymphocytic infiltrates in the salivary and lachrymal glands. Such infiltrates are comprised of activated lymphocytes and macrophages, and known to produce multiple cytokines including interferon-gamma (IFN-γ). In this study, we have demonstrated that IFN-γ strongly induces the expression of immunoproteasome beta subunits (β1i, β2i and β5i) and immunoproteasome activity but conversely inhibits the expression of proteasome beta subunits (β1, β2 and β5) in human salivary gland (HSG) cells. Mass spectrometric analysis has revealed potential MHC I-associated peptides on the HSG cells, including a tryptic peptide derived from salivary amylase, due to IFN-γ stimulation. These results suggest that IFN-γ induces immunoproteasomes in HSG cells, leading to enhanced presentation of MHC I-associated peptides on cell surface. These peptide-presenting salivary gland cells may be recognized and targeted by auto-reactive T lymphocytes. We have also found that lactacystin, a proteasome inhibitor, inhibits the expression of β1 subunit in HSG cells and blocks the IFN-γ-induced expression of β1i and immunoproteasome activity. However, the expression of β2i and β5i in HSG cells is not affected by lactacystin. These results may add new insight into the mechanism regarding how lactacystin blocks the action of proteasomes or immunoproteasomes.
authors.affiliation: School of Dentistry and Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, California, United States of America.
authors.forename: Martha E
authors.initials: ME
authors.lastname: Arellano-Garcia
authors.affiliation: School of Dentistry and Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, California, United States of America.
authors.forename: Kaori
authors.initials: K
authors.lastname: Misuno
authors.affiliation: Faculty of Dentistry, McGill University, Montreal, Quebec, Canada.
authors.forename: Simon D
authors.initials: SD
authors.lastname: Tran
authors.affiliation: School of Dentistry and Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, California, United States of America.
authors.forename: Shen
authors.initials: S
authors.lastname: Hu
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008285:Major Histocompatibility Complex; D013058:Mass Spectrometry; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D021122:Protein Subunits; C067713:lactacystin; D007371:Interferon-gamma; C077436:LMP7 protein; D046988:Proteasome Endopeptidase Complex; D000111:Acetylcysteine
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