title: Colonization of the meat extracellular matrix proteins by O157 and non-O157 enterohemorrhagic Escherichia coli.
abstract: Enterohemorrhagic Escherichia coli (EHEC) are anthropozoonotic agents that range third among food-borne pathogens respective to their incidence and dangerousness in the European Union. EHEC are Shiga-toxin producing E. coli (STEC) responsible for foodborne poisoning mainly incriminated to the consumption of contaminated beef meat. Among the hundreds of STEC serotypes identified, EHEC mainly belong to O157:H7 but non-O157 can represent 20 to 70% of EHEC infections per year. Seven of those serogroups are especially of high-risk for human health, i.e. O26, O45, O103, O111, O121, O145 and O104. While meat can be contaminated all along the food processing chain, EHEC contamination essentially occurs at the dehiding stage of slaughtering. Investigating bacterial colonization to the skeletal-muscle extracellular matrix (ECM) proteins, it appeared that environmental factors influenced specific and non-specific bacterial adhesion of O157 and non-O157 EHEC as well as biofilm formation. Importantly, mechanical treatment (i.e. shaking, centrifugation, pipetting and vortexing) inhibited and biased the results of bacterial adhesion assay. Besides stressing the importance of the protocol to investigate bacterial adhesion to ECM proteins, this study demonstrated that the colonization abilities to ECM proteins vary among EHEC serogroups and should ultimately be taken into consideration to evaluate the risk of contamination for different types of food matrices.
authors.affiliation: INRA, UR454 Microbiologie, F-63122 Saint-Genès Champanelle, France; INRA, UR370 Qualité des Produits Animaux, F-63122 Saint-Genès Champanelle, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Chagnot
authors.affiliation: INRA, UR454 Microbiologie, F-63122 Saint-Genès Champanelle, France.
authors.forename: Nelly
authors.initials: N
authors.lastname: Caccia
authors.affiliation: Université de Lyon, VetAgro Sup, LMAP Laboratory, National Reference Laboratory for E. coli (including STEC), F-69280 Marcy l'Etoile, France; Université de Lyon, UMR 5557 Ecologie Microbienne, Université Lyon 1, CNRS, VetAgro Sup, Research Group on Bacterial Opportunistic Pathogens and Environment, F-69622 Villeurbanne, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Loukiadis
authors.affiliation: Université de Lyon, VetAgro Sup, LMAP Laboratory, National Reference Laboratory for E. coli (including STEC), F-69280 Marcy l'Etoile, France; Université de Lyon, UMR 5557 Ecologie Microbienne, Université Lyon 1, CNRS, VetAgro Sup, Research Group on Bacterial Opportunistic Pathogens and Environment, F-69622 Villeurbanne, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Ganet
authors.affiliation: INRA, UR454 Microbiologie, F-63122 Saint-Genès Champanelle, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Durand
authors.affiliation: INRA, UR454 Microbiologie, F-63122 Saint-Genès Champanelle, France.
authors.forename: Yolande
authors.initials: Y
authors.lastname: Bertin
authors.affiliation: INRA, UR454 Microbiologie, F-63122 Saint-Genès Champanelle, France.
authors.forename: Régine
authors.initials: R
authors.lastname: Talon
authors.affiliation: INRA, UR370 Qualité des Produits Animaux, F-63122 Saint-Genès Champanelle, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Astruc
authors.affiliation: INRA, UR454 Microbiologie, F-63122 Saint-Genès Champanelle, France. Electronic address: mickael.desvaux@clermont.inra.fr.
authors.forename: Mickaël
authors.initials: M
authors.lastname: Desvaux
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D001427:Bacterial Toxins / Q000235:genetics; D018441:Biofilms; D002417:Cattle; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics / Q000254:growth & development*; D019453:Escherichia coli O157 / Q000235:genetics / Q000254:growth & development*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005516:Food Microbiology; D006801:Humans; D008460:Meat / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; D016326:Extracellular Matrix Proteins; D037521:Virulence Factors
keywords: Bacterial adhesion; Biofilm formation; Enterohemorrhagic Escherichia coli; Extracellular matrix protein; Meat contamination; Shiga-toxin producing E. coli