title: Quantitation of sugar content in pyrolysis liquids after acid hydrolysis using high-performance liquid chromatography without neutralization.
abstract: A rapid method for the quantitation of total sugars in pyrolysis liquids using high-performance liquid chromatography (HPLC) was developed. The method avoids the tedious and time-consuming sample preparation required by current analytical methods. It is possible to directly analyze hydrolyzed pyrolysis liquids, bypassing the neutralization step usually required in determination of total sugars. A comparison with traditional methods was used to determine the validity of the results. The calibration curve coefficient of determination on all standard compounds was >0.999 using a refractive index detector. The relative standard deviation for the new method was 1.13%. The spiked sugar recoveries on the pyrolysis liquid samples were between 104 and 105%. The research demonstrates that it is possible to obtain excellent accuracy and efficiency using HPLC to quantitate glucose after acid hydrolysis of polymeric and oligomeric sugars found in fast pyrolysis bio-oils without neutralization.
authors.affiliation: Bioeconomy Institute and ‡Department of Mechanical Engineering, Iowa State University , Ames, Iowa 50011, United States.
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Johnston
authors.forename: Robert C
authors.initials: RC
authors.lastname: Brown
mesh_terms: D000821:Animal Feed / Q000032:analysis; D056804:Biofuels / Q000191:economics; D002138:Calibration; D002424:Caustics / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003246:Conservation of Energy Resources; D005947:Glucose / Q000032:analysis; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009005:Monosaccharides / Q000032:analysis*; D009844:Oligosaccharides / Q000737:chemistry*; D008897:Panicum / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012031:Refractometry; D015203:Reproducibility of Results; D012995:Solubility; D013464:Sulfuric Acids / Q000737:chemistry*
chemical_list: D056804:Biofuels; D002424:Caustics; D009005:Monosaccharides; D009844:Oligosaccharides; D010938:Plant Oils; D011134:Polysaccharides; D013464:Sulfuric Acids; D005947:Glucose; C033158:sulfuric acid