title: Potent in vitro synergism of fusidic acid (FA) and berberine chloride (BBR) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA).
abstract: It was found in the present study that combined use of fusidic acid (FA) and berberine chloride (BBR) offered an in vitro synergistic action against 7 of the 30 clinical methicillin-resistant Staphylococcus aureus (MRSA) strains, with a fractional inhibitory concentration (FIC) index ranging from 0.5 to 0.19. This synergistic effect was most pronounced on MRSA 4806, an FA-resistant isolate, with a minimum inhibitory concentration (MIC) value of 1,024 μg/ml. The time-kill curve experiment showed that FA plus BBR yielded a 4.2 log10 c.f.u./ml reduction in the number of MRSA 4806 bacteria after 24-h incubation as compared with BBR alone. Viable count analysis showed that FA plus BBR produced a 3.0 log10 c.f.u./ml decrease in biofilm formation and a 1.5 log10 c.f.u./ml decrease in mature biofilm in viable cell density as compared with BBR alone. In addition, phase contrast micrographs confirmed that biofilm formation was significantly inhibited and mature biofilm was obviously destructed when FA was used in combination with BBR. These results provide evidence that combined use of FA and BBR may prove to be a promising clinical therapeutic strategy against MRSA.
authors.affiliation: Department of Clinical Pharmacy, General Hospital of Chengdu Military Command Region, 270 Rongdu Road, Chengdu, 610083, People's Republic of China, rmliang2718@163.com.
authors.forename: Rong-mei
authors.initials: RM
authors.lastname: Liang
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Yong
authors.forename: Yu-qin
authors.initials: YQ
authors.lastname: Duan
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Tan
authors.forename: Ping
authors.initials: P
authors.lastname: Zeng
authors.forename: Zi-ying
authors.initials: ZY
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Shi-hua
authors.initials: SH
authors.lastname: Wang
authors.forename: Yun-ping
authors.initials: YP
authors.lastname: Jiang
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Huang
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Dong
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Hu
authors.forename: Hui-qing
authors.initials: HQ
authors.lastname: Shi
authors.forename: Nan
authors.initials: N
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D058491:Bacterial Load; D001599:Berberine / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D004357:Drug Synergism; D005672:Fusidic Acid / Q000494:pharmacology*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D013203:Staphylococcal Infections / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001599:Berberine; D005672:Fusidic Acid
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