title: Optimization of the condition for adsorption of gallic acid by Aspergillus oryzae mycelia using Box-Behnken design.
abstract: Fresh biomass of Aspergillus oryzae (A. oryzae) CGMCC5992 can effectively remove gallic acid from aqueous solution. To improve the removal rate of gallic acid, this study first identified the important factors affecting the removal rate of gallic acid with univariate analysis, and then used four-factor and three-level Box-Behnken design (BBD) with the removal rate of gallic acid as response value, to obtain the optimum conditions for the removal of gallic acid as follows: 6.95 h treatment time, pH 3.70, 7.07 g/L mycelium volume, and 120.64 mg/L initial concentration of gallic acid. Under such optimized condition, the removal rate of gallic acid approached 99.21 %. HPLC-MS analysis proved that the gallic acid in aqueous solution was completely removed by A. oryzae, rather than being metabolized into its derivatives. Scanning electron microscopy (SEM) indicated that the biomass morphology and surface structure of A. oryzae changed after the adsorption of gallic acid. Thus, the present study has provided an optimal condition for A. oryzae removal of gallic acid in water.
authors.affiliation: Institute of Agro-production Processing Engineering, Jiangsu University, Xuefu Road 301, Zhenjiang, 212013, People's Republic of China, zhangtamei@163.com.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qiaoxia
authors.initials: Q
authors.lastname: Pang
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Huihua
authors.initials: H
authors.lastname: Zheng
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D001236:Aspergillus oryzae / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D002851:Chromatography, High Pressure Liquid; D052918:Environmental Restoration and Remediation / Q000295:instrumentation / Q000379:methods*; D005707:Gallic Acid / Q000032:analysis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D025282:Mycelium / Q000254:growth & development / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D014874:Water Pollutants, Chemical; D005707:Gallic Acid
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