title: Methionine protects against hyperthermia-induced cell injury in cultured bovine mammary epithelial cells.
abstract: The aim of this study was to investigate the effects of methionine on cell proliferation, antioxidant activity, apoptosis, the expression levels of related genes (HSF-1, HSP70, Bax and Bcl-2) and the expression levels of protein (HSP70) in mammary epithelial cells, after heat treatment. Methionine (60 mg/L) increased the viability and attenuated morphological damage in hyperthermia-treated bovine mammary epithelial cells (BMECs). Additionally, methionine significantly reduced lactate dehydrogenase leakage, malondialdehyde formation, nitric oxide, and nitric oxide synthase activity. Superoxide dismutase, catalase, and glutathione peroxidase enzymatic activity was increased significantly in the presence of methionine. Bovine mammary epithelial cells also exhibited a certain amount of HSP70 reserve after methionine pretreatment for 24 h, and the expression level of the HSP70 gene and protein further increased with incubation at 42 °C for 30 min. Compared to the control, the expression of HSF-1 mRNA increased, and there was a significantly reduced expression of Bax/Bcl-2 mRNA and a reduced activity of caspase-3 against heat stress. Methionine also increased survival and decreased early apoptosis of hyperthermia-treated BMECs. Thus, methionine has cytoprotective effects on hyperthermia-induced damage in BMECs.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, 1# Weigang, Nanjing, 210095, China, zyhan6708@njau.edu.cn.
authors.forename: Zhao-Yu
authors.initials: ZY
authors.lastname: Han
authors.forename: Tian
authors.initials: T
authors.lastname: Mu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D000076249:Heat Shock Transcription Factors; D006979:Hyperthermia, Induced; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects*; D008715:Methionine / Q000494:pharmacology*; D009569:Nitric Oxide / Q000032:analysis; D010088:Oxidoreductases / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D014157:Transcription Factors; D051028:bcl-2-Associated X Protein; D009569:Nitric Oxide; D008715:Methionine; D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3
references.citation: J Dairy Sci. 1993 Oct;76(10):3079-98
references.pmid: 8227632
references.citation: Trends Cell Biol. 2001 Jan;11(1):6-10
references.pmid: 11146277
references.citation: Cancer Lett. 2002 May 8;179(1):51-8
references.pmid: 11880182
references.citation: Int J Hyperthermia. 2006 May;22(3):191-6
references.pmid: 16754338
references.citation: J Orthop Sci. 2002;7(1):117-22
references.pmid: 11819143
references.citation: J Neurosci. 2000 Nov 15;20(22):8384-9
references.pmid: 11069945
references.citation: J Dairy Sci. 2005 Feb;88(2):660-70
references.pmid: 15653533
references.citation: J Appl Physiol (1985). 2002 Apr;92(4):1725-42
references.pmid: 11896043
references.citation: Genes Dev. 1999 Aug 1;13(15):1899-911
references.pmid: 10444588
references.citation: J Natl Cancer Inst. 1990 Oct 17;82(20):1628-32
references.pmid: 2213904
references.citation: Gene Expr. 1999;7(4-6):271-82
references.pmid: 10440228
references.citation: Ann Med. 1999 Aug;31(4):261-71
references.pmid: 10480757
references.citation: Cell Biol Int. 2014 Aug;38(8):971-6
references.pmid: 24604888
references.citation: J Dairy Sci. 1998 Feb;81(2):482-91
references.pmid: 9532503
references.citation: Ann N Y Acad Sci. 2005 May;1042:471-80
references.pmid: 15965093
references.citation: Cell. 1997 Feb 21;88(4):471-81
references.pmid: 9038338
references.citation: Immunol Today. 1997 Jan;18(1):44-51
references.pmid: 9018974
references.citation: Ann N Y Acad Sci. 1998 Jun 30;851:67-74
references.pmid: 9668607
references.citation: Cell. 1997 Nov 14;91(4):479-89
references.pmid: 9390557
references.citation: Annu Rev Genet. 1988;22:631-77
references.pmid: 2853609
references.citation: Mutagenesis. 2001 Jul;16(4):317-22
references.pmid: 11420399
references.citation: J Dairy Sci. 2008 Feb;91(2):445-54
references.pmid: 18218730
references.citation: Sheng Wu Gong Cheng Xue Bao. 2007 May;23(3):471-6
references.pmid: 17577996
references.citation: Pancreas. 2000 Nov;21(4):352-7
references.pmid: 11075989
references.citation: J Dairy Sci. 1999 Sep;82(9):1935-42
references.pmid: 10509252
references.citation: J Biol Chem. 2002 Mar 15;277(11):9219-25
references.pmid: 11741880
references.citation: Mol Cell. 2010 Feb 12;37(3):299-310
references.pmid: 20159550
references.citation: Nature. 2000 Oct 12;407(6805):770-6
references.pmid: 11048727
references.citation: Biochimie. 2002 Feb-Mar;84(2-3):153-66
references.pmid: 12022946
references.citation: Poult Sci. 2011 Jul;90(7):1555-61
references.pmid: 21673172
references.citation: Ecotoxicol Environ Saf. 2008 Sep;71(1):301-8
references.pmid: 18261796
references.citation: Annu Rev Pharmacol Toxicol. 1998;38:63-96
references.pmid: 9597149
references.citation: Int J Hyperthermia. 2005 Dec;21(8):681-7
references.pmid: 16338849
references.citation: Science. 1973 Feb 9;179(4073):588-90
references.pmid: 4686466
references.citation: Annu Rev Cell Dev Biol. 1995;11:441-69
references.pmid: 8689565
references.citation: Jpn J Cancer Res. 1999 Feb;90(2):180-7
references.pmid: 10189888
references.citation: Curr Opin Oncol. 2009 Nov;21(6):516-23
references.pmid: 19730103
references.citation: J Endocrinol. 1987 Dec;115(3):439-45
references.pmid: 3443806
references.citation: Methods Enzymol. 1990;186:407-21
references.pmid: 2233308
references.citation: Methods Enzymol. 1984;105:121-6
references.pmid: 6727660