title: Do spawn storage conditions influence the colonization capacity of a wheat-straw-based substrate by Agaricus subrufescens?
abstract: Storage conditions of the spawn of edible fungi are of major importance to facilitate the production of mushrooms. Here, standard storage conditions at 10°C or 15°C were used and the potential of colonization of standard European compost by the tropical species Agaricus subrufescens was assessed during the spawn running phase. Two lignocellulolytic activities, laccase and CMC-cellulase, were enhanced after storage compared to control as well as substrate transformation, as described by the aromaticity ratio and a humification ratio calculated from NMR data. This result indicates that mycelium growth probably occurred during storage at 10 or 15°C, leading to a larger amount of biomass in the inoculum. Moreover, the microbial functional diversity of the substrate was favored, showing that the electivity of the substrate was maintained. Thus, these findings indicate that recommendations for the mushroom producers can be established for A. subrufescens cultivation under European standard conditions.
authors.affiliation: Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Aix Marseille université, UMR CNRS, IRD, Avignon université, faculté de Saint-Jérôme, 13397 Marseille cedex 20, France. Electronic address: anne-marie.farnet@imbe.fr.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Farnet
authors.affiliation: Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Aix Marseille université, UMR CNRS, IRD, Avignon université, faculté de Saint-Jérôme, 13397 Marseille cedex 20, France.
authors.forename: Leila
authors.initials: L
authors.lastname: Qasemian
authors.affiliation: Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Aix Marseille université, UMR CNRS, IRD, Avignon université, faculté de Saint-Jérôme, 13397 Marseille cedex 20, France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Peter-Valence
authors.affiliation: Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Aix Marseille université, UMR CNRS, IRD, Avignon université, faculté de Saint-Jérôme, 13397 Marseille cedex 20, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Ruaudel
authors.affiliation: INRA, UR 1264 MycSA, Mycologie et sécurité des aliments, BP 81, 33883 Villenave-d'Ornon, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Savoie
authors.affiliation: Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Aix Marseille université, UMR CNRS, IRD, Avignon université, faculté de Saint-Jérôme, 13397 Marseille cedex 20, France.
authors.forename: Sevastianos
authors.initials: S
authors.lastname: Roussos
authors.affiliation: Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Aix Marseille université, UMR CNRS, IRD, Avignon université, faculté de Saint-Jérôme, 13397 Marseille cedex 20, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Gaime-Perraud
authors.affiliation: TRACES, Campus de l'Étoile, 13397, Aix-Marseille université, Marseille cedex 20, France.
authors.forename: Fabio
authors.initials: F
authors.lastname: Ziarelli
authors.affiliation: Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Aix Marseille université, UMR CNRS, IRD, Avignon université, faculté de Saint-Jérôme, 13397 Marseille cedex 20, France.
authors.forename: Élisée
authors.initials: É
authors.lastname: Ferré
mesh_terms: D000363:Agaricales / Q000254:growth & development*; D000364:Agaricus / Q000254:growth & development*; D000383:Agriculture; D001419:Bacteria / Q000254:growth & development; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D008660:Metabolism; D025282:Mycelium / Q000254:growth & development; D012987:Soil; D013696:Temperature; D014908:Triticum / Q000382:microbiology
chemical_list: D012987:Soil
keywords: Almond mushroom; Champignon amande; Culture de champignons; Enzymes lignocellulolytiques; Lignocellulolytic enzymes; Mushroom cultivation; RMN du solide du (13)C; Solid-state NMR of (13)C