title: Tick burden and prevalence of Theileria parva infection in Tarime zebu cattle in the lake zone of Tanzania.
abstract: This study was carried out to assess the distribution, abundance of different tick genera and prevalence of Theileria parva infection in Tarime zebu cattle kept in selected wards of Serengeti and Tarime districts in Mara region. Adult ticks were identified and counted from half body parts of 360 animals which were extensively managed in communal land with natural pastures. Concurrently, blood samples were collected and thereafter DNA extracted and a nested polymerase chain reaction (nPCR) was done using primers specific for p104 gene to detect the presence of T. parva DNA. Ticks were identified into four groups: Amblyomma genus, Boophilus sub-genus of Rhipicephalus genus, other species of Rhipicephalus, and Hyalomma genus. Rhipicephalus genus accounted for 71.8 % of the total ticks, whereas Amblyomma, Boophilus sub-genus of Rhipicephalus genus and Hyalomma constituted 14.1, 14.0 and 0.1 %, respectively. There were more animals (p < 0.05) infested with ticks in Tarime district (96.1 %) than in Serengeti (61.7 %). The average counts of ticks were higher in adult animals (p < 0.05) than in young animals. The overall prevalence of T. parva was 27.7 % and was higher (p < 0.05) in Serengeti (38.3 %) than in Tarime district (16.7 %). However, all animals tested positive for T. parva did not show any clinical signs of East Coast fever (ECF), suggesting the existence of subclinical infection in Tarime zebu. These results suggest that Tarime cattle can tolerate ECF infection and are likely to serve as potential carriers of T. parva to other less-tolerant cattle breeds in mixed herds. Since Tarime cattle are preferred by most farmers with mixed herds, routine screening for T. parva is highly recommended to minimize introduction of infected cattle into an immunologically naive population.
authors.affiliation: Inspectorate Department, Ministry of Education and Vocational Training, Eastern Zone, P.O. Box 325, Morogoro, Tanzania, emalaisser@gmail.com.
authors.forename: Emmanuel Levillal Katamboi
authors.initials: EL
authors.lastname: Laisser
authors.forename: Maulilio John
authors.initials: MJ
authors.lastname: Kipanyula
authors.forename: George
authors.initials: G
authors.lastname: Msalya
authors.forename: Robinson Hammerthon
authors.initials: RH
authors.lastname: Mdegela
authors.forename: Esron Daniel
authors.initials: ED
authors.lastname: Karimuribo
authors.forename: Anjello Joseph
authors.initials: AJ
authors.lastname: Mwilawa
authors.forename: Elisa Daniel
authors.initials: ED
authors.lastname: Mwega
authors.forename: Lughano
authors.initials: L
authors.lastname: Kusiluka
authors.forename: Sebastian Wilson
authors.initials: SW
authors.lastname: Chenyambuga
mesh_terms: D000818:Animals; D002417:Cattle; D026863:Ixodidae; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D013636:Tanzania / Q000453:epidemiology; D016797:Theileria parva / Q000302:isolation & purification*; D013801:Theileriasis / Q000453:epidemiology*; D013984:Tick Infestations / Q000453:epidemiology / Q000662:veterinary*
references.citation: Vet J. 2007 Sep;174(2):390-6
references.pmid: 17015024
references.citation: Onderstepoort J Vet Res. 2012 Sep 03;79(1):E1-E10
references.pmid: 23327317
references.citation: Mol Biochem Parasitol. 1990 Feb;39(1):47-60
references.pmid: 1689460
references.citation: Parasit Vectors. 2012 Sep 07;5:194
references.pmid: 22958352
references.citation: Trop Anim Health Prod. 2006 May;38(4):291-9
references.pmid: 17137131
references.citation: Exp Appl Acarol. 1997 Mar;21(3):179-92
references.pmid: 9127491
references.citation: Onderstepoort J Vet Res. 1979 Mar;46(1):31-9
references.pmid: 460821
references.citation: Parasitol Res. 2010 Jan;106(2):357-65
references.pmid: 19902251
references.citation: Vet Parasitol. 1994 Apr;52(3-4):243-56
references.pmid: 8073608
references.citation: Trop Anim Health Prod. 2005 Aug;37(6):479-89
references.pmid: 16248219
references.citation: Vet Ital. 2012 Oct-Dec;48(4):379-85
references.pmid: 23277119