title: Healing efficacy of an EGF impregnated triple gel based wound dressing: in vitro and in vivo studies.
abstract: To accomplish an ideal wound healing process which promotes healthy tissue growth with less scaring, a novel gel based topical drug delivery system composed of 3 different polymers chitosan, dextran sulfate, and polyvinylpyrrolidone K30 (CDP) was prepared. The physicochemical properties of the prepared gels were investigated in vitro. Gels showed a maximum swelling ratio of 50 ± 1.95 times of dried gel in PBS at pH 7.4. The swelling ratios increase in acidic and alkaline pH to 55.3 ± 1.75 and 65.5 ± 2.42, respectively. In the rheological test, prepared gels revealed viscoelastic properties and a small linear viscoelastic region of 0.166%. In vivo wound healing promoting activities of CDP gels containing 20 μg/mL EGF were evaluated on surgically induced dermal wounds in rats using pathologic examination. The application of CDP gel with incorporated EGF significantly reduced the defect on the rat's skin and enhanced epithelial healing compared with the topical application of the EGF-free CDP gel. The results clearly substantiate the beneficial effects of the topical application of CDP containing EGF in the acceleration of healthy wound healing process with less scarring.
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.
authors.forename: Najmeh
authors.initials: N
authors.lastname: Khanbanha
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran ; Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Atyabi
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.
authors.forename: Azade
authors.initials: A
authors.lastname: Taheri
authors.affiliation: Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Talaie
authors.affiliation: Noor Ophthalmology Research Center, Noor Eye Hospital, P.O. Box 19393-3475, Tehran, Iran.
authors.forename: Mirgholamreza
authors.initials: M
authors.lastname: Mahbod
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran ; Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.
authors.forename: Rassoul
authors.identifier: 0000-0003-0694-7556
authors.initials: R
authors.lastname: Dinarvand
mesh_terms: D000818:Animals; D001458:Bandages; D002152:Calorimetry, Differential Scanning; D048271:Chitosan / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005782:Gels / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011205:Povidone / Q000737:chemistry; D017208:Rats, Wistar; D012212:Rheology / Q000187:drug effects; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D003911:Dextrans; D005782:Gels; D004815:Epidermal Growth Factor; D048271:Chitosan; D011205:Povidone
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