title: Genomic aberrations in myeloid sarcoma without blood or bone marrow involvement: characterization of formalin-fixed paraffin-embedded samples by chromosomal microarrays.
abstract: Myeloid sarcoma (MS) is a presentation of acute myeloid leukemia (AML) as a tumor mass outside of the bone marrow. Viable cells from MS are frequently unavailable for cytogenetic studies. We therefore investigated whether chromosomal microarray analysis (CMA) using formalin-fixed paraffin-embedded (FFPE) tissues can detect clinically important genetic abnormalities in MS. CMA successfully identified genomic aberrations in six cases of MS, and in two cases it revealed multiple abnormalities equivalent to a complex karyotype, thus predicting a poor outcome. CMA using FFPE material is therefore a feasible and clinically applicable approach for detection of prognostically significant genomic abnormalities in MS.
authors.affiliation: Department of Pathology, The University of Chicago, Chicago, USA.
authors.forename: M Kamran
authors.initials: MK
authors.lastname: Mirza
authors.affiliation: Department of Medicine, The University of Chicago, Chicago, USA.
authors.forename: Madina
authors.initials: M
authors.lastname: Sukhanova
authors.affiliation: Department of Internal Medicine, University Hospital Carl Gustav Carus, University of Technology, Dresden, Germany.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Stölzel
authors.affiliation: Department of Pediatrics, The University of Chicago, Chicago, USA.
authors.forename: Kenan
authors.initials: K
authors.lastname: Onel
authors.affiliation: Department of Medicine, The University of Chicago, Chicago, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Larson
authors.affiliation: Department of Medicine, The University of Chicago, Chicago, USA.
authors.forename: Wendy
authors.initials: W
authors.lastname: Stock
authors.affiliation: Department of Internal Medicine, University Hospital Carl Gustav Carus, University of Technology, Dresden, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Ehninger
authors.affiliation: Department of Pathology, University Hospital Carl Gustav Carus, University of Technology, Dresden, Germany.
authors.forename: Friederike
authors.initials: F
authors.lastname: Kuithan
authors.affiliation: Department of Nuclear Medicine, University Hospital Carl Gustav Carus, University of Technology, Dresden, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Zöphel
authors.affiliation: Department of Pathology, The University of Chicago, Chicago, USA.
authors.forename: Poluru
authors.initials: P
authors.lastname: Reddy
authors.affiliation: Department of Pathology, The University of Chicago, Chicago, USA.
authors.forename: Loren
authors.initials: L
authors.lastname: Joseph
authors.affiliation: Department of Medicine, The University of Chicago, Chicago, USA. Electronic address: graca@bsd.uchicago.edu.
authors.forename: Gordana
authors.initials: G
authors.lastname: Raca
mesh_terms: D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology; D006801:Humans; D008297:Male; D046228:Microarray Analysis / Q000379:methods*; D008875:Middle Aged; D016612:Paraffin Embedding; D023981:Sarcoma, Myeloid / Q000097:blood / Q000235:genetics* / Q000473:pathology; D016707:Tissue Fixation / Q000379:methods
chemical_list: D005557:Formaldehyde
keywords: Chromosomal microarrays; Cytogenetics; Myeloid sarcoma
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