title: Functional MRI in mice lacking IP3-dependent calcium signaling in astrocytes.
abstract: Functional magnetic resonance imaging (fMRI) is a fundamental tool to investigate human brain networks. However, the cellular mechanisms underlying fMRI signals are not fully understood. One hypothetical mechanism is the putative vascular control exerted by cytosolic calcium in perivascular astrocytes. We have performed combined fMRI-electrophysiology experiments in mice lacking the inositol 1,4,5-triphosphate-type-2 receptor, with the primary pathway of cytosolic calcium increase eliminated into astrocytes. Our results show that evoked electrophysiologic activity and fMRI signals acquired during either transient or sustained neuronal activations occur independently of these large calcium signals. This result challenges the suggested intermediary role of astrocytic calcium surges in fMRI-signal generation.
authors.affiliation: Centre of Excellence Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Miguel Hernández, Sant Joan d'Alacant, Spain.
authors.forename: Pierrick
authors.initials: P
authors.lastname: Jego
authors.affiliation: Centre of Excellence Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Miguel Hernández, Sant Joan d'Alacant, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Pacheco-Torres
authors.affiliation: 1] Instituto Cajal, CSIC, Madrid, Spain [2] Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Araque
authors.affiliation: Centre of Excellence Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Miguel Hernández, Sant Joan d'Alacant, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Canals
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D020013:Calcium Signaling; D004558:Electric Stimulation; D055724:Electrophysiological Phenomena; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000235:genetics / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019580:Perforant Pathway
chemical_list: D053496:Inositol 1,4,5-Trisphosphate Receptors; D015544:Inositol 1,4,5-Trisphosphate
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