title: Sub-lethal heat stress causes apoptosis in an Antarctic fish that lacks an inducible heat shock response.
abstract: The endemic fish fauna of the Southern Ocean are cold-adapted stenotherms and are acutely sensitive to elevated temperature. Many of these species lack a heat shock response and cannot increase the production of heat shock proteins in their tissues. However, some species retain the ability to induce other stress-responsive genes, some of which are involved in cell cycle arrest and apoptosis. Here, the effect of heat on cell cycle stage and its ability to induce apoptosis were tested in thermally stressed hepatocytes from a common Antarctic fish species from McMurdo Sound in the Ross Sea. Levels of proliferating cell nuclear antigen were also measured as a marker of progression through the cell cycle. The results of these studies demonstrate that even sub-lethal heat stress can have deleterious impacts at the cellular level on these environmentally sensitive species.
authors.affiliation: Center for Life in Extreme Environments, Department of Biology, Portland State University, Portland, OR 97207, United States.
authors.forename: Isaac M
authors.initials: IM
authors.lastname: Sleadd
authors.affiliation: Center for Life in Extreme Environments, Department of Biology, Portland State University, Portland, OR 97207, United States.
authors.forename: Marissa
authors.initials: M
authors.lastname: Lee
authors.affiliation: Center for Life in Extreme Environments, Department of Biology, Portland State University, Portland, OR 97207, United States.
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Hassumani
authors.affiliation: Center for Life in Extreme Environments, Department of Biology, Portland State University, Portland, OR 97207, United States.
authors.forename: Tonya M A
authors.initials: TM
authors.lastname: Stecyk
authors.affiliation: Center for Life in Extreme Environments, Department of Biology, Portland State University, Portland, OR 97207, United States.
authors.forename: Otto K
authors.initials: OK
authors.lastname: Zeitz
authors.affiliation: Center for Life in Extreme Environments, Department of Biology, Portland State University, Portland, OR 97207, United States. Electronic address: bbuckley@pdx.edu.
authors.forename: Bradley A
authors.initials: BA
authors.lastname: Buckley
mesh_terms: D000064:Acclimatization; D000818:Animals; D000864:Antarctic Regions; D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response; D022781:Hepatocytes / Q000378:metabolism / Q000502:physiology; D010473:Perciformes / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D018809:Proliferating Cell Nuclear Antigen
keywords: Antarctic fish; Cell death; Thermal stress