title: [Callus induction and plant regeneration of Sambucus chinensis].
abstract: OBJECTIVE
  To optimize the medium for callus and multiple shoot induction and cultural plantlets rooting of Sambucus chinensis, and to provide a basis for the development of the seedling of mass production and germplasm conservation.
  
  
  METHODS
  The plantlet leaves, petioles, stems and shoot tips were used as explants for the study of influences of different explants and different hormones on callus and multiple shoot induction, and cultural plantlets rooting.
  
  
  RESULTS
  The optimal medium for callus induction of leaves was MS +2, 4-D 1.5 mg/L; and optimum mediums for petiole were MS + NAA 0.2 mg/L + KT 1.0 mg/L and MS +2, 4-D 0. 2 mg/L + KT 1.0 mg/L, MS + NAA 0.2 mg/L + KT 3.0 mg/L + GA, 2.0 mg/L medium was best for multiple shoot induction. And the best medium for tissue culture rooting was 1/2 MS + NAA 0. 3 mg/L. On the optimized medium, the roots were well-developed and the plants were healthier.
  
  
  CONCLUSIONS
  Both the leaves and petioles can be well induced to callus on suitable medium, the stems and shoot tips are the best materials for multiple shoot induction and plant regeneration.
authors.forename: Qing-gui
authors.initials: QG
authors.lastname: Wu
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Zou
authors.forename: Ming-hua
authors.initials: MH
authors.lastname: Luo
mesh_terms: D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development; D012038:Regeneration / Q000187:drug effects*; D027923:Sambucus / Q000187:drug effects / Q000254:growth & development*; D036226:Seedlings / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators