title: Chronic sleep deprivation-induced proteome changes in astrocytes of the rat hypothalamus.
abstract: Sleep deprivation (SD) can influence cognition, memory, and sleep/wake homeostasis and can cause impairments in many physiological processes. Because the homeostatic control of the sleep/wake cycle is closely associated with the hypothalamus, the current study was undertaken to examine proteomic changes occurring in hypothalamic astrocytes following chronic partial SD. After chronic partial SD for 7 days, astrocytes were prepared from rat hypothalamus using a Percoll gradient method, and their proteome profiles were determined by LC-MS/MS. Comparisons of the proteome profiles of hypothalamic astrocytes revealed that chronic partial SD increased (≥1.5-fold) 89 proteins and decreased (≤0.7-fold) 50 proteins; these changes in protein expression were validated by western blot or immunohistochemistry. DAVID and IPA analyses of these proteins suggested that SD may influence gliotransmission and astrocyte activation. PPP2R1A, RTN4, VAMP-2, LGI-1, and SLC17A7 were identified and validated as the main targets of SD in astrocytes. Our results suggest that SD may modulate gliotransmission in the hypothalamus, thereby disturbing sleep/wake homeostasis and increasing susceptibility to neurological disease; however, further studies are required to confirm whether the proteome changes are specific to SD.
authors.affiliation: Department of Pharmacology, Brain Science & Engineering Institute, BK21 PLUS KNU Biomedical Convergence Program for Creative Talent, ‡Department of Molecular Medicine, Cell & Matrix Research Institute, §Department of Neurology, Brain Science & Engineering Institute, Kyungpook National University School of Medicine , Daegu 700-422, Republic of Korea.
authors.forename: Jae-Hong
authors.initials: JH
authors.lastname: Kim
authors.forename: Jong-Heon
authors.initials: JH
authors.lastname: Kim
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Cho
authors.forename: Moon-Chang
authors.initials: MC
authors.lastname: Baek
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Maan-Gee
authors.initials: MG
authors.lastname: Lee
authors.forename: Il-Sung
authors.initials: IS
authors.lastname: Jang
authors.forename: Ho-Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Kyoungho
authors.initials: K
authors.lastname: Suk
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000378:metabolism*; D007031:Hypothalamus / Q000166:cytology* / Q000378:metabolism; D008297:Male; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012892:Sleep Deprivation / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome