title: Molecular signal suppression by in situ microextraction in nuclear magnetic resonance spectroscopy.
abstract: The detailed characterization of complex mixtures by NMR is often hampered by the presence of signals from uninformative compounds, the resonances of which overlap with those of the molecules of interest. We provide here a proof of principle for an approach to NMR signal suppression in complex samples using Molecularly Imprinted Polymers (MIPS). Addition of a few milligrams of polymer to a solution traps the target molecule in typical micromolar to millimolar concentration, thus achieving in situ signal suppression, without altering any other spectral features. This method minimized any manipulation or perturbation of the spectrum and was applied to a complex mixture of known compounds and to a plant extract, in both cases spiked with a compound (bisphenol A), which was subsequently removed by selective binding to a complementary MIP. What is described in this report is comparable with microextraction and may in due course be applied to a large number of analytical challenges.
authors.affiliation: Aix Marseille Université, Centrale Marseille, CNRS, iSm2 UMR 7313, 13397, Marseille, France; Research Group Biosynthesis/NMR Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745, Jena, Germany.
authors.forename: Jima
authors.initials: J
authors.lastname: Chandran
authors.forename: Laetitia
authors.initials: L
authors.lastname: Shintu
authors.forename: Stefano
authors.initials: S
authors.lastname: Caldarelli
mesh_terms: D000814:Aniline Compounds / Q000032:analysis*; D001559:Benzhydryl Compounds / Q000032:analysis*; D001593:Benzyl Compounds / Q000032:analysis*; D001713:Biphenyl Compounds / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D054802:Molecular Imprinting; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis*; D011108:Polymers / Q000737:chemistry; D052617:Solid Phase Microextraction; D000098826:Bisphenol A Compounds
chemical_list: D000814:Aniline Compounds; D001559:Benzhydryl Compounds; D001593:Benzyl Compounds; D001713:Biphenyl Compounds; D010636:Phenols; D011108:Polymers; C010574:biphenyl; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C023650:aniline
keywords: Molecularly Imprinted Polymers; mixture analysis; nuclear magnetic resonance; signal suppression