title: In vitro study of a novel nanogold-collagen composite to enhance the mesenchymal stem cell behavior for vascular regeneration.
abstract: Novel nanocomposites based on type I collagen (Col) containing a small amount (17.4, 43.5, and 174 ppm) of gold nanoparticles (AuNPs, approximately 5 nm) were prepared in this study. The pure Col and Col-AuNP composites (Col-Au) were characterized by the UV-Vis spectroscopy (UV-Vis), surface-enhanced raman spectroscopy (SERS) and atomic force microscopy (AFM). The interaction between Col and AuNPs was confirmed by infrared (IR) spectra. The effect of AuNPs on the biocompatibility of Col, evaluated by the proliferation and reactive oxygen species (ROS) production of mesenchymal stem cells (MSCs) as well as the activation of monocytes and platelets, was investigated. Results showed that Col-Au had better biocompatibility than Col. Upon stimulation by vascular endothelial growth factor (VEGF) and stromal derived factor-1α (SDF-1α), MSCs expressed the highest levels of αvβ3 integrin/CXCR4, focal adhesion kinase (FAK), matrix metalloproteinase-2 (MMP-2), and Akt/endothelial nitric oxide synthase (eNOS) proteins when grown on the Col-Au (43.5 ppm) nanocomposite. Taken together, Col-Au nanocomposites may promote the proliferation and migration of MSCs and stimulate the endothelial cell differentiation. These results suggest that Col-Au may be used to construct tissue engineering scaffolds for vascular regeneration.
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan. R.O.C.; Center for Neuropsychiatry, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Huey-Shan
authors.initials: HS
authors.lastname: Hung
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan. R.O.C.
authors.forename: Chih-Hsuan
authors.initials: CH
authors.lastname: Chang
authors.affiliation: Central Taiwan University of Science and Technology, Department of Medical Imaging and Radiological Science, Taichung, Taiwan, R.O.C.
authors.forename: Chen-Jung
authors.initials: CJ
authors.lastname: Chang
authors.affiliation: Institute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Cheng-Ming
authors.initials: CM
authors.lastname: Tang
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan. R.O.C.
authors.forename: Wei-Chien
authors.initials: WC
authors.lastname: Kao
authors.affiliation: Center for Neuropsychiatry, China Medical University Hospital, Taichung, Taiwan, R.O.C.; China Medical University Beigang Hospital, Yunlin, Taiwan, R.O.C.; Graduate Institute of Immunology, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Shinn-Zong
authors.initials: SZ
authors.lastname: Lin
authors.affiliation: Blood Bank, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Hsien-Hsu
authors.initials: HH
authors.lastname: Hsieh
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan. R.O.C.
authors.forename: Mei-Yun
authors.initials: MY
authors.lastname: Chu
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan. R.O.C.
authors.forename: Wei-Shen
authors.initials: WS
authors.lastname: Sun
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan, R.O.C.; Rehabilitation Engineering Research Center, National Taiwan University, Taipei, Taiwan, R.O.C.
authors.forename: Shan-Hui
authors.initials: SH
authors.lastname: Hsu
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D017612:Gold Compounds / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry; D012038:Regeneration / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D017612:Gold Compounds; D003094:Collagen
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