title: Mycobacterium abscessus infection in cystic fibrosis: molecular typing and clinical outcomes.
abstract: Mycobacterium abscessus is a significant pathogen in the cystic fibrosis patient population. PCR amplification and sequencing can provide accurate subspecies identification, and can predict macrolide susceptibility, which is becoming increasingly important for patient management. Molecular techniques for further typing of isolates provide tools for the ongoing investigations into the clinical impact of particular M. abscessus strains. Whole-genome sequencing is likely to be the only technique that provides sufficient resolution for investigating transmission events between patients.
authors.affiliation: Microbiology, Virology and Infection Control Department, Great Ormond Street Hospital NHS Foundation Trust, London, UK.
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Harris
authors.affiliation: Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England, London, UK.
authors.forename: Dervla T D
authors.initials: DTD
authors.lastname: Kenna
mesh_terms: D003550:Cystic Fibrosis / Q000150:complications*; D006801:Humans; D058889:Molecular Typing; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology* / Q000635:transmission; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016896:Treatment Outcome