title: A combination of methylprednisolone and quercetin is effective for the treatment of cardiac contusion following blunt chest trauma in rats.
abstract: Cardiac contusion is a potentially fatal complication of blunt chest trauma. The effects of a combination of quercetin and methylprednisolone against trauma-induced cardiac contusion were studied. Thirty-five female Sprague-Dawley rats were divided into five groups (n=7) as follows: sham, cardiac contusion with no therapy, treated with methylprednisolone (30 mg/kg on the first day, and 3 mg/kg on the following days), treated with quercetin (50 mg·kg(-1)·day(-1)), and treated with a combination of methylprednisolone and quercetin. Serum troponin I (Tn-I) and tumor necrosis factor-alpha (TNF-α) levels and cardiac histopathological findings were evaluated. Tn-I and TNF-α levels were elevated after contusion (P=0.001 and P=0.001). Seven days later, Tn-I and TNF-α levels decreased in the rats treated with methylprednisolone, quercetin, and the combination of methylprednisolone and quercetin compared to the rats without therapy, but a statistical significance was found only with the combination therapy (P=0.001 and P=0.011, respectively). Histopathological degeneration and necrosis scores were statistically lower in the methylprednisolone and quercetin combination group compared to the group treated only with methylprednisolone (P=0.017 and P=0.007, respectively). However, only degeneration scores were lower in the combination therapy group compared to the group treated only with quercetin (P=0.017). Inducible nitric oxide synthase positivity scores were decreased in all treatment groups compared to the untreated groups (P=0.097, P=0.026, and P=0.004, respectively). We conclude that a combination of quercetin and methylprednisolone can be used for the specific treatment of cardiac contusion.
authors.affiliation: Department of Pediatric Cardiology, Faculty of Medicine, Dicle University, Diyarbak?r, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Demir
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Ondokuz May?s University, Samsun, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Güzel
authors.affiliation: Department of Emergency Medicine, Faculty of Medicine, Ondokuz May?s University, Samsun, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Kat
authors.affiliation: Pediatric Cardiology Services, Behçet Uz Children's Hospital, ?zmir, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Karadeniz
authors.affiliation: Department of Emergency Medicine, Faculty of Medicine, Ondokuz May?s University, Samsun, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Akdemir
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Ondokuz May?s University, Samsun, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Okuyucu
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Ondokuz May?s University, Samsun, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Gacar
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Ondokuz May?s University, Samsun, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Özdemir
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Ondokuz May?s University, Samsun, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Güvenç
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D000975:Antioxidants / Q000627:therapeutic use; D003288:Contusions / Q000188:drug therapy* / Q000209:etiology; D004359:Drug Therapy, Combination; D005260:Female; D006335:Heart Injuries / Q000188:drug therapy* / Q000209:etiology; D007150:Immunohistochemistry; D008775:Methylprednisolone / Q000627:therapeutic use*; D009206:Myocardium / Q000473:pathology*; D009336:Necrosis; D052247:Nitric Oxide Synthase Type II / Q000302:isolation & purification; D011794:Quercetin / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D013898:Thoracic Injuries / Q000150:complications; D019210:Troponin I / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014949:Wounds, Nonpenetrating / Q000150:complications*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D019210:Troponin I; D014409:Tumor Necrosis Factor-alpha; D011794:Quercetin; D052247:Nitric Oxide Synthase Type II; D008775:Methylprednisolone
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