title: Enhanced inactivation of food-borne pathogens in ready-to-eat sliced ham by near-infrared heating combined with UV-C irradiation and mechanism of the synergistic bactericidal action.
abstract: The objective of the study described in this article was, first, to investigate the effect of the simultaneous application of near-infrared (NIR) heating and UV irradiation on inactivation of Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes in ready-to-eat (RTE) sliced ham and as well as its effect on product quality and, second, to elucidate the underlying mechanisms of the synergistic bactericidal action of NIR heating and UV irradiation. With the inoculation amounts used, simultaneous NIR-UV combined treatment for 70 s achieved 3.62, 4.17, and 3.43 log CFU reductions of E. coli O157:H7, S. Typhimurium, and L. monocytogenes, respectively. For all three pathogens, the simultaneous application of both technologies resulted in an additional log unit reduction as a result of their synergism compared to the sum of the reductions obtained after the individual treatments. To investigate the mechanisms of NIR-UV synergistic injury for a particular microorganism in a food base, we evaluated the effect of four types of metabolic inhibitors using the overlay method and confirmed that damage to cellular membranes and the inability of cells to repair these structures due to ribosomal damage were the primary factors related to the synergistic lethal effect. Additionally, NIR-UV combined treatment for a maximum of 70 s did not alter the color values or texture parameters of ham slices significantly (P > 0.05). These results suggest that a NIR-UV combined process could be an innovative antimicrobial intervention for RTE meat products.
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Institute of GreenBio Science and Technology, Seoul National University, Seoul, South Korea.
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Ha
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Institute of GreenBio Science and Technology, Seoul National University, Seoul, South Korea Kang7820@snu.ac.kr.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kang
mesh_terms: D015169:Colony Count, Microbial; D004203:Disinfection / Q000379:methods*; D019453:Escherichia coli O157 / Q000502:physiology / Q000528:radiation effects*; D005516:Food Microbiology / Q000379:methods*; D006358:Hot Temperature; D008089:Listeria monocytogenes / Q000502:physiology / Q000528:radiation effects*; D008461:Meat Products / Q000382:microbiology*; D050296:Microbial Viability / Q000528:radiation effects*; D012486:Salmonella typhimurium / Q000502:physiology / Q000528:radiation effects*; D014466:Ultraviolet Rays
references.citation: Int J Food Microbiol. 2010 Mar 31;138(1-2):50-5
references.pmid: 20116875
references.citation: Appl Environ Microbiol. 1999 Dec;65(12):5334-7
references.pmid: 10583985
references.citation: Appl Environ Microbiol. 2012 Dec;78(23):8353-61
references.pmid: 23001665
references.citation: Food Microbiol. 2014 Apr;38:263-9
references.pmid: 24290650
references.citation: J Food Prot. 2006 Jan;69(1):71-9
references.pmid: 16416903
references.citation: Appl Environ Microbiol. 1980 Nov;40(5):939-49
references.pmid: 6160814
references.citation: Annu Rev Microbiol. 1993;47:321-50
references.pmid: 7504905
references.citation: Appl Environ Microbiol. 2012 Sep;78(18):6458-65
references.pmid: 22773635
references.citation: Food Chem Toxicol. 2004 Feb;42(2):245-59
references.pmid: 14667471
references.citation: Food Microbiol. 2008 Sep;25(6):735-44
references.pmid: 18620965
references.citation: Appl Environ Microbiol. 2013 Nov;79(21):6568-75
references.pmid: 23956394
references.citation: J Food Prot. 2010 Nov;73(11):2103-9
references.pmid: 21219726
references.citation: Meat Sci. 2009 Dec;83(4):599-603
references.pmid: 20416652
references.citation: J Gen Microbiol. 1989 Jul;135(7):1941-7
references.pmid: 2693606
references.citation: Nat Rev Microbiol. 2010 Jun;8(6):423-35
references.pmid: 20440275
references.citation: J Gen Microbiol. 1976 Nov;97(1):19-27
references.pmid: 792394
references.citation: Cell. 2007 Sep 7;130(5):797-810
references.pmid: 17803904
references.citation: J Food Prot. 2012 May;75(5):896-905
references.pmid: 22564939
references.citation: Appl Environ Microbiol. 2014 Mar;80(6):1858-63
references.pmid: 24413596
references.citation: Lancet. 1996 Apr 13;347(9007):1053-4
references.pmid: 8606603
references.citation: Appl Environ Microbiol. 1987 Jun;53(6):1358-64
references.pmid: 2440382
references.citation: J Bacteriol. 1971 Feb;105(2):512-8
references.pmid: 4925192
references.citation: J Bacteriol. 1976 Feb;125(2):429-34
references.pmid: 173708
references.citation: Food Microbiol. 2014 Aug;41:27-32
references.pmid: 24750810
references.citation: J Food Prot. 2003 Jun;66(6):1030-4
references.pmid: 12801005