title: Detection and molecular characterization of porcine circovirus type 2 from piglets with porcine circovirus associated diseases in Colombia.
abstract: BACKGROUND
  The porcine circovirus-associated disease (PCVAD) has been known since 1991 in Canada, but the first outbreak of PCVAD in Colombia was reported in 2007. In order to understand the molecular epidemiology of the disease and to establish the origin of the virus in the country, the study presented here intended to evaluate the presence of PCV2-associated systemic infection in piglets from different geographical regions over a period of 9-years (2002-2010). The analysis included samples collected before, during and after outbreaks of PCVAD in pigs from Colombia. The PCV2 ORF2 from the positive samples was sequenced and used to determine the genotypes of the strains and to study the dynamic of these genotypes throughout the time.
  
  
  RESULTS
  PCV2 DNA was detected in cases related to PCV2-associated systemic infection as well as in healthy pigs with a presumable persistent infection. The analysis of the ORF2 nucleotide full length sequence of twenty-three strains allowed to divide them into two groups: PCV2a and PCV2b. At the amino acid level the main variations in the sequence of the capsid protein were found in regions located within the immunoreactive areas.
  
  
  CONCLUSIONS
  The results of this study demonstrated for the first time, that the two subgroups: PCV2a and PCV2b have been circulating in swine from Colombia. In addition, the study showed that genotype PCV2b is present in Colombian pigs suffering from both clinical and presumable persistent infection and that the PCV2b genotype was present in the Colombian pig population even before recognition of the disease in the country and it became predominant through time.
authors.affiliation: Colombian Agriculture Institute - ICA, National Laboratory of Veterinary Diagnostic, Bogotá D,C, Colombia. marinconm@unal.edu.co.
authors.forename: Maria Antonia
authors.initials: MA
authors.lastname: Rincón Monroy
authors.forename: Gloria Consuelo
authors.initials: GC
authors.lastname: Ramirez-Nieto
authors.forename: Victor Julio
authors.initials: VJ
authors.lastname: Vera
authors.forename: Jairo Jaime
authors.initials: JJ
authors.lastname: Correa
authors.forename: Jose Dario
authors.initials: JD
authors.lastname: Mogollón-Galvis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification / Q000302:isolation & purification*; D003105:Colombia / Q000453:epidemiology; D004279:DNA, Viral / Q000302:isolation & purification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins
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