title: Effect of lactoferrin-impregnated porous poly(lactide-co-glycolide) (PLGA) microspheres on osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs).
abstract: The aim of this study was to develop lactoferrin (LF)-impregnated porous poly(lactide-co-glycolide) (PLGA) microspheres (PMs) to induce osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). Porous PLGA PMs were fabricated by a fluidic device and their surfaces were modified with heparin-dopamine (Hep-DOPA). Then, LF (100μg, 500μg, and 1000μg) was impregnated on the surface of heparinized PMs (Hep-PMs) via electrostatic interactions to yield LF-impregnated PMs. PMs and modified PMs were characterized by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Osteogenic differentiation of rADSCs on PMs and modified PMs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and mRNA expression of osteocalcin and osteopontin. Successful immobilization of Hep-DOPA and LF on the surface of PMs was confirmed by XPS analysis. LF-impregnated PMs generated significantly greater ALP activity, calcium deposition, and mRNA expression of osteocalcin and osteopontin compared with PMs. These results suggested that LF-impregnated PMs effectively induced osteogenic differentiation of rADSCs.
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Yun
authors.affiliation: Department of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu 136-701, Republic of Korea.
authors.forename: Kyu-Sik
authors.initials: KS
authors.lastname: Shim
authors.affiliation: Division of Bio-imaging, Chuncheon Center, Korea Basic Science Institute, 192-1 Hyoja 2-dong, Chuncheon, Gangwon-do 200-701, Republic of Korea.
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do 420-743, Republic of Korea.
authors.forename: Sung-Wook
authors.initials: SW
authors.lastname: Choi
authors.affiliation: Department of Orthopedic Surgery, Korea University Medical College, Ansan Hospital, Gojan 1-dong, Danwon-gu, Gyeonggi-do 425-707, Republic of Korea. Electronic address: mayhap@hanmail.net.
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: Suh
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D017931:DNA Primers; D019344:Lactic Acid / Q000737:chemistry*; D007781:Lactoferrin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D015675:Osteocalcin / Q000235:genetics; D010012:Osteogenesis; D053495:Osteopontin / Q000235:genetics; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D055672:Static Electricity; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D015675:Osteocalcin; D053495:Osteopontin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D007781:Lactoferrin; D002118:Calcium
keywords: Fluidic device; Lactoferrin; Osteogenic differentiation; Porous microspheres; Rabbit adipose-derived stem cells