title: Substrate recognition of holocytochrome c synthase: N-terminal region and CXXCH motif of mitochondrial cytochrome c.
abstract: Holocytochrome c synthase (HCCS) attaches heme covalently to mitochondrial respiratory cytochromes c. Little is known about the reaction of heme attachment to apocytochromes c by HCCS, although recently it has been established that the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important protein-protein recognition motifs. Here, we explore further the important features of the N-terminal sequence and investigate what variations in the CXXCH residues are productively recognised by HCCS in its substrate.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom. Electronic address: julie.stevens@bioch.ox.ac.uk.
authors.forename: Julie M
authors.initials: JM
authors.lastname: Stevens
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom. Electronic address: stuart.ferguson@bioch.ox.ac.uk.
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Ferguson
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D017124:Conserved Sequence; D045304:Cytochromes c / Q000737:chemistry* / Q000235:genetics; D006736:Horses; D008190:Lyases / Q000737:chemistry*; D024101:Mitochondrial Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D011485:Protein Binding; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: C014059:CYC1 protein, S cerevisiae; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D008190:Lyases; C031581:cytochrome C synthetase
keywords: Ccm system; Cytochrome c; Heme; Holocytochrome c synthase
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