title: Effect of heat shock protein 90 (Hsp90) on migration and invasion of human cancer cells in vitro.
abstract: We studied the effect of purified native heat shock protein 90 (Hsp90) from bovine and mouse brain on migration and invasion of human glioblastoma (A-172) and fibrosarcoma (HT1080) cells. Hsp90 in concentrations of 0.01-0.10 mg/ml stimulated migration and invasion of tumor cells in vitro by 20-32% (p<0.05). Polyclonal antibodies to Hsp90 blocked the Hsp90-dependent stimulation of cell invasion, which indicates specificity of the stimulating effect of extracellular Hsp90 on tumor cell invasion. Hence, extracellular Hsp90 can be considered as a promising molecular target, because its inhibition can suppress invasion and metastasizing of tumor cells.
authors.affiliation: Institute of Cell Biophysics of Russian Academy of Sciences, Pushchino, Russia, snigireva.s@gmail.com.
authors.forename: A V
authors.initials: AV
authors.lastname: Snigireva
authors.forename: V V
authors.initials: VV
authors.lastname: Vrublevskaya
authors.forename: Yu Yu
authors.initials: YY
authors.lastname: Skarga
authors.forename: Yu V
authors.initials: YV
authors.lastname: Evdokimovskaya
authors.forename: O S
authors.initials: OS
authors.lastname: Morenkov
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001923:Brain Chemistry; D002417:Cattle; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D015194:Diffusion Chambers, Culture; D016326:Extracellular Matrix Proteins / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D018840:HSP70 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects*
chemical_list: D000906:Antibodies; D016326:Extracellular Matrix Proteins; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins