title: Molecular characterization of two positive-strand RNA viruses co-infecting a hypovirulent strain of Sclerotinia sclerotiorum.
abstract: Two dsRNA segments, the replicative forms of two ssRNA viruses of SsHV2/SX247 (Sclerotinia sclerotiorum hypovirus 2) and SsDRV/SX247 (Sclerotinia sclerotiorum debilitation-associated RNA virus), were isolated from the hypovirulent strain SX247 of Sclerotinia sclerotiorum. SsDRV/SX247 has the highest similarities (81% aa identity) with the previously reported virus SsDRV/Ep-1PN. The genome of SsHV2/SX247 is 15,219bp in length with a poly-A tail, and it has only one large putative open reading frame (ORF) that encodes a polyprotein containing RNA-dependent RNA polymerase (RdRp) and viral RNA helicase domains. The RdRp domain shares amino acid similarity with that of CHV1 (23%). However, the genome organization of SsHV2/SX247 is significantly different from that of CHV1 on genomic size and ORFs. We conclude that SsDRV/SX247 is a novel strain in species SsDRV of genus Sclerodarnavirus, whereas SsHV2/SX247 is a representative member of new proposed lineage Gammahypovirus in the family Hypoviridae and confers hypovirulence in its host.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Zijin
authors.initials: Z
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Songsong
authors.initials: S
authors.lastname: Wu
authors.affiliation: The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Jiasen
authors.initials: J
authors.lastname: Cheng
authors.affiliation: The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
authors.forename: Daohong
authors.initials: D
authors.lastname: Jiang
authors.affiliation: The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China. Electronic address: Jiataoxie@mail.hzau.edu.cn.
authors.forename: Jiatao
authors.initials: J
authors.lastname: Xie
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000472:pathogenicity / Q000821:virology*; D029741:Brassica rapa / Q000382:microbiology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D012328:RNA Viruses / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014774:Virulence
chemical_list: D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase
keywords: Gammahypovirus; Hypoviridae; Hypovirulence; Hypovirus; Sclerodarnavirus; Sclerotinia sclerotiorum