title: Assessment of body composition in Indian adults: comparison between dual-energy X-ray absorptiometry and isotope dilution technique.
abstract: Dual-energy X-ray absorptiometry (DXA) and isotope dilution technique have been used as reference methods to validate the estimates of body composition by simple field techniques; however, very few studies have compared these two methods. We compared the estimates of body composition by DXA and isotope dilution (18O) technique in apparently healthy Indian men and women (aged 19-70 years, n 152, 48 % men) with a wide range of BMI (14-40 kg/m2). Isotopic enrichment was assessed by isotope ratio mass spectroscopy. The agreement between the estimates of body composition measured by the two techniques was assessed by the Bland-Altman method. The mean age and BMI were 37 (sd 15) years and 23·3 (sd 5·1) kg/m2, respectively, for men and 37 (sd 14) years and 24·1 (sd 5·8) kg/m2, respectively, for women. The estimates of fat-free mass were higher by about 7 (95 % CI 6, 9) %, those of fat mass were lower by about 21 (95 % CI - 18, - 23) %, and those of body fat percentage (BF%) were lower by about 7·4 (95 % CI - 8·2, - 6·6) % as obtained by DXA compared with the isotope dilution technique. The Bland-Altman analysis showed wide limits of agreement that indicated poor agreement between the methods. The bias in the estimates of BF% was higher at the lower values of BF%. Thus, the two commonly used reference methods showed substantial differences in the estimates of body composition with wide limits of agreement. As the estimates of body composition are method-dependent, the two methods cannot be used interchangeably.
authors.affiliation: School of Exercise and Nutrition Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology,Brisbane,QLD,Australia.
authors.forename: Bharati
authors.initials: B
authors.lastname: Kulkarni
authors.affiliation: Department of Non-communicable Disease Epidemiology,London School of Hygiene and Tropical Medicine,London,UK.
authors.forename: Hannah
authors.initials: H
authors.lastname: Kuper
authors.affiliation: School of Social and Community Medicine, University of Bristol,Bristol,UK.
authors.forename: Amy
authors.initials: A
authors.lastname: Taylor
authors.affiliation: Childhood Nutrition Research Centre, UCL Institute of Child Health,London,UK.
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Wells
authors.affiliation: Clinical Division, National Institute of Nutrition,Jamai Osmania PO,Hyderabad500 007,India.
authors.forename: K V
authors.initials: KV
authors.lastname: Radhakrishna
authors.affiliation: Department of Non-communicable Disease Epidemiology,London School of Hygiene and Tropical Medicine,London,UK.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kinra
authors.affiliation: School of Social and Community Medicine, University of Bristol,Bristol,UK.
authors.forename: Yoav
authors.initials: Y
authors.lastname: Ben-Shlomo
authors.affiliation: School of Social and Community Medicine, University of Bristol,Bristol,UK.
authors.forename: George Davey
authors.initials: GD
authors.lastname: Smith
authors.affiliation: Department of Non-communicable Disease Epidemiology,London School of Hygiene and Tropical Medicine,London,UK.
authors.forename: Shah
authors.initials: S
authors.lastname: Ebrahim
authors.affiliation: Saint John's Research Institute,Bangalore,India.
authors.forename: A V
authors.initials: AV
authors.lastname: Kurpad
authors.affiliation: School of Exercise and Nutrition Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology,Brisbane,QLD,Australia.
authors.forename: Nuala M
authors.initials: NM
authors.lastname: Byrne
authors.affiliation: Mater Mothers' Hospital, Mater Research Institute - University of Queensland and Griffith Health Institute, Griffith University,Brisbane,QLD,Australia.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Hills
mesh_terms: D015502:Absorptiometry, Photon; D000328:Adult; D000368:Aged; D001823:Body Composition; D001827:Body Height; D015992:Body Mass Index; D001835:Body Weight; D005260:Female; D006801:Humans; D007194:India; D007201:Indicator Dilution Techniques; D008297:Male; D008875:Middle Aged; D010103:Oxygen Isotopes; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D010103:Oxygen Isotopes
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