title: External NAD(P)H dehydrogenases in Acanthamoeba castellanii mitochondria.
abstract: The mitochondrial respiratory chain of plants and some fungi contains multiple rotenone-insensitive NAD(P)H dehydrogenases, of which at least two are located on the outer surface of the inner membrane (i.e., external NADH and external NADPH dehydrogenases). Annotated sequences of the putative alternative NAD(P)H dehydrogenases of the protozoan Acanthamoeba castellanii demonstrated similarity to plant and fungal sequences. We also studied activity of these dehydrogenases in isolated A. castellanii mitochondria. External NADPH oxidation was observed for the first time in protist mitochondria. The coupling parameters were similar for external NADH oxidation and external NADPH oxidation, indicating similar efficiencies of ATP synthesis. Both external NADH oxidation and external NADPH oxidation had an optimal pH of 6.8 independent of relevant ubiquinol-oxidizing pathways, the cytochrome pathway or a GMP-stimulated alternative oxidase. The maximal oxidizing activity with external NADH was almost double that with external NADPH. However, a lower Michaelis constant (K(M)) value for external NADPH oxidation was observed compared to that for external NADH oxidation. Stimulation by Ca(2+) was approximately 10 times higher for external NADPH oxidation, while NADH dehydrogenase(s) appeared to be slightly dependent on Ca(2+). Our results indicate that external NAD(P)H dehydrogenases similar to those in plant and fungal mitochondria function in mitochondria of A. castellanii.
authors.affiliation: Department of Bioenergetics, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland.
authors.forename: Nina
authors.initials: N
authors.lastname: Antos-Krzeminska
authors.affiliation: Department of Bioenergetics, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland. Electronic address: wiesiaj@amu.edu.pl.
authors.forename: Wieslawa
authors.initials: W
authors.lastname: Jarmuszkiewicz
mesh_terms: D048248:Acanthamoeba castellanii / Q000201:enzymology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism; D020536:Enzyme Activators / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D051336:Mitochondrial Membranes / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D002413:Cations, Divalent; D020536:Enzyme Activators; D000255:Adenosine Triphosphate; D009252:NADPH Dehydrogenase; D009245:NADH Dehydrogenase; D002118:Calcium
keywords: Acanathamoeba castellanii; external NAD(P)H dehydrogenases; mitochondria; respiratory activities.