title: Species identification of strains belonging to genus Citrobacter using the biochemical method and MALDI-TOF mass spectrometry.
abstract: Strains of genus Citrobacter (152 isolates from 1950 to 1988 deposited in the Czech National Collection of Type Cultures, Prague) were re-classified using biological and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) methods. One-hundred thirty-six strains (ca. 90 %) were identified to the species level using the biological method with evaluation by Farmer matrix. MALDI-TOF MS exhibited better identification capability, the data being more compact; the method was unambiguously successful in typing 145 (95 %) strains. Comparison of the results of identification by the two methods revealed differences (for 12 samples) in identified species which, considering all biochemical and/or MS characteristics, could be attributed to the natural variability of strains and close relation of the misidentified species (all of them belonged to the Citrobacter freundii complex). Taking into account all the above data, both methods can be considered reliable; however, the MALDI-TOF MS exhibits higher accuracy, efficiency, and rapidity.
authors.affiliation: National Institute of Public Health, Šrobárova 48, 100 42, Prague 10, Czech Republic, rkolinska@szu.cz.
authors.forename: Renáta
authors.initials: R
authors.lastname: Kolínská
authors.forename: Petra
authors.initials: P
authors.lastname: Spanělová
authors.forename: Michal
authors.initials: M
authors.lastname: Dřevínek
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Hrabák
authors.forename: Helena
authors.initials: H
authors.lastname: Zemličková
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D001671:Biochemistry / Q000379:methods*; D002954:Citrobacter / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
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