title: Detection of DNA damage in peripheral blood mononuclear cells from pancreatic cancer patients.
abstract: DNA repair is a key mechanism in maintaining genomic stability: repair deficiencies increase DNA damage and mutations that lead to several diseases, including cancer. We extracted DNA from peripheral blood mononuclear cells (PBMCs) of 48 pancreatic adenocarcinoma cases and 48 healthy controls to determine relative levels of nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) damage by QPCR. All participants were never smokers and between the ages of 60 and 69. Average levels among cases were compared to controls using a rank sum test, and logistic regression adjusted for potential confounding factors (age, sex, and diabetes mellitus). Cases had less DNA damage, with a significant decrease in mtDNA damage (P-value = 0.03) and a borderline significant decrease in nDNA damage (P = 0.08). Across samples, we found mtDNA abundance was higher among non-diabetics compared to diabetics (P = 0.04). Our results suggest that patients with pancreatic adenocarcinoma have less DNA damage in their PBMCs, and that having diabetes, a known pancreatic cancer risk factor, is associated with lower levels of mtDNA abundance.
authors.affiliation: Department of Health Sciences Research, Division of Epidemiology, Mayo Clinic, Rochester, Minnesota.
authors.forename: Rick J
authors.initials: RJ
authors.lastname: Jansen
authors.affiliation: Department of Physiology and Biophysics, University of Puerto Rico, San Juan, Puerto Rico.
authors.forename: Sharon
authors.initials: S
authors.lastname: Fonseca-Williams
authors.affiliation: Department of Health Sciences Research, Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota.
authors.forename: William R
authors.initials: WR
authors.lastname: Bamlet
authors.affiliation: Department of Pharmacology and Toxicology, University of Puerto Rico, San Juan, Puerto Rico.
authors.forename: Sylvette
authors.initials: S
authors.lastname: Ayala-Peña
authors.affiliation: Department of Health Sciences Research, Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota.
authors.forename: Ann L
authors.initials: AL
authors.lastname: Oberg
authors.affiliation: Department of Health Sciences Research, Division of Epidemiology, Mayo Clinic, Rochester, Minnesota.
authors.forename: Gloria M
authors.initials: GM
authors.lastname: Petersen
authors.affiliation: Department of Physiology and Biophysics, University of Puerto Rico, San Juan, Puerto Rico.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Torres-Ramos
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D000368:Aged; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial
keywords: DNA damage; QPCR; pancreatic cancer
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