title: [Quantitative determination of 5 active ingredients in different harvest periods of Ligusticum chuanxiong by HPLC].
abstract: A simple and quick method is described for the determination of ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A and ligustilide in rhizomes of Ligusticum chuanxiong. The 5 active ingredients in the sample was extracted using 40% ethanol and analyzed by reversed-phase high performance liquid chromatography (HPLC). Chromatography separation was performed using Agilent 1100 series HPLC system with a Symmetry C18 column and gradient elution with a mixture of three solvents : solvent A, acetonitrile, solvent B, methanol and solvent C, 1% aqueous acetic acid, 0 min to 5 min A: B: C 20: 40: 40, 5 min to 30 min A: B: C 60 to 100 : 0 : 40 to 0. The effluent was monitored using a VWD detector set at 321 nm (0-4.3 min) and 275 nm (4.31-30 min). The flow rate was set at 1 mL x min(-1) and the injection volume was 10 microL. The column temperature was maintained at 35 degrees C. The calibration curve was linear (r > or = 0.99) over the tested ranges. The average recovery was 94.44%-103.1% (n = 6). The method has been successfully applied to the analysis in different harvest periods of L. chuanxiong samples. In this paper, single-factor randomized block design to study the 5 components content of L. chuanxiong on ten collecting stages. For the L. chuanxiong collected from April 15th to May 30rd, the content of 5 ingredients increased primarily, and then decreased. Determine the appropriate harvest time has important significance to the promotion of the quality of L. chuanxiong.
authors.forename: Jin-Liang
authors.initials: JL
authors.lastname: Liu
authors.forename: Qiao-Jia
authors.initials: QJ
authors.lastname: Fan
authors.forename: Shun-Lin
authors.initials: SL
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: Tan
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.forename: Ji-Chao
authors.initials: JC
authors.lastname: Yuan
authors.forename: Shi-Min
authors.initials: SM
authors.lastname: Yang
authors.forename: Fan-Lei
authors.initials: FL
authors.lastname: Kong
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis; D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D001572:Benzofurans / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis*; D030004:Ligusticum / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D000097:Acetonitriles; D001572:Benzofurans; D003373:Coumaric Acids; D012997:Solvents; C584604:senkyunolide H; C576743:senkyunolide I; C027820:ligustilide; C045855:3-N-butyl-4,5-dihydrophthalide; C004999:ferulic acid; D015107:4-Butyrolactone; D019342:Acetic Acid; D000432:Methanol; C032159:acetonitrile