title: 5-Aminosalicylic acid attenuates allergen-induced airway inflammation and oxidative stress in asthma.
abstract: Pro-inflammatory cytokines regulate the magnitude of allergic reactions during asthma. Tumor necrosis factor--alpha (TNF-α), interleukin-6 (IL-6) and interleukin-13 (IL-13) play a crucial role in aggravating the inflammatory conditions during allergic asthma. In addition, oxidative stress contributes to the pathogenesis of asthma by altering the physiological condition resulting in the development of status asthmaticus. Anti-inflammatory corticosteroids are being widely used for treating allergic asthma. In the present study 5-aminosalicylic acid (5-ASA), a salicylic acid derivative, was evaluated, in vivo for its potential to suppress TNF-α, IL-6 and IL-13 using ovalbumin (OVA) induced allergic asthma in Balb/C mice. Oral administration of 65, 130 and 195 mg/kg 5-ASA significantly reduced the OVA induced total and differential leucocyte count, TNF-α, IL-6, IL-13, nitrite, nitrate, MDA, MPO and TPL levels in the lung lavage samples. Collectively, these findings suggest that 5-ASA is a potent immunomodulator and suppresses key Th2 cytokines production and oxidative stress in OVA-induced asthma.
authors.affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamandalam, The Nilgiris 643001, Tamilnadu, India. Electronic address: ramasatyanarayanarajuk@gmail.com.
authors.forename: K Rama Satyanarayana
authors.initials: KR
authors.lastname: Raju
authors.affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamandalam, The Nilgiris 643001, Tamilnadu, India.
authors.forename: M N Sathish
authors.initials: MN
authors.lastname: Kumar
authors.affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamandalam, The Nilgiris 643001, Tamilnadu, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Udhagamandalam, India.
authors.forename: Srinivas T
authors.initials: ST
authors.lastname: Naga
authors.affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamandalam, The Nilgiris 643001, Tamilnadu, India.
authors.forename: Jaya K
authors.initials: JK
authors.lastname: Shankar
authors.affiliation: Molecular Pharmacology and Therapeutics, Mayo Clinic, Rochester, MN, USA.
authors.forename: Vishakantha
authors.initials: V
authors.lastname: Murthy
authors.affiliation: Department of Biochemistry, JSS Medical College, JSS University, Mysore, India.
authors.forename: Subba Rao V
authors.initials: SR
authors.lastname: Madhunapanthula
authors.affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamandalam, The Nilgiris 643001, Tamilnadu, India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Mulukutla
authors.affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamandalam, The Nilgiris 643001, Tamilnadu, India.
authors.forename: Nilesh S
authors.initials: NS
authors.lastname: Ambhore
authors.affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamandalam, The Nilgiris 643001, Tamilnadu, India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Tummala
authors.affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamandalam, The Nilgiris 643001, Tamilnadu, India.
authors.forename: V J
authors.initials: VJ
authors.lastname: Vishnuvarthan
authors.affiliation: Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Udhagamandalam, India.
authors.forename: Afzal
authors.initials: A
authors.lastname: Azam
authors.affiliation: Department of Pharmacology, JSS College of Pharmacy, Udhagamandalam, The Nilgiris 643001, Tamilnadu, India.
authors.forename: Kannan
authors.initials: K
authors.lastname: Elango
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D007249:Inflammation / Q000188:drug therapy; D018793:Interleukin-13 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D008168:Lung / Q000737:chemistry; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D019804:Mesalamine / Q000627:therapeutic use*; D008807:Mice, Inbred BALB C; D009566:Nitrates / Q000032:analysis; D009573:Nitrites / Q000032:analysis; D010047:Ovalbumin / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D018793:Interleukin-13; D015850:Interleukin-6; D009566:Nitrates; D009573:Nitrites; D014409:Tumor Necrosis Factor-alpha; D019804:Mesalamine; D008315:Malondialdehyde; D010047:Ovalbumin; D009195:Peroxidase
keywords: 5-Aminosalicylic acid; Allergic asthma; Inflammation; Oxidative stress; Proinflammatory cytokines