title: A novel cost-effective assay based on real-time PCR for COMT Val158Met genotyping.
abstract: A functional polymorphism in the catechol-O-methyltransferase (COMT) gene (Val158Met) has been associated with a large number of human diseases and endophenotypes. The aim of this study was to develop a novel cost-effective assay to genotype this polymorphism. The novel assay was based on the combination of allele-specific PCR and high-resolution melting in a qPCR platform. Melt-curve analysis allowed a clear differentiation of the three genotypes. This novel assay could be implemented in the study of a large number of diseases and endophenotypes related to COMT dysfunction and could be extended for the analysis of other functional polymorphisms.
authors.affiliation: Laboratory of NeuroPsychiatric Genetics, Biomedical Sciences Research Group, School of Medicine, Universidad Antonio Nariño , Bogotá , Colombia .
authors.forename: Diego A
authors.initials: DA
authors.lastname: Ojeda
authors.forename: Sandra
authors.initials: S
authors.lastname: López-León
authors.forename: Diego A
authors.initials: DA
authors.lastname: Forero
mesh_terms: D002394:Catechol O-Methyltransferase / Q000235:genetics*; D003362:Cost-Benefit Analysis; D005820:Genetic Testing / Q000191:economics* / Q000379:methods; D005838:Genotype; D017048:Health Care Costs; D006801:Humans; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction / Q000191:economics*; D015203:Reproducibility of Results
chemical_list: C588010:COMT protein, human; D002394:Catechol O-Methyltransferase
keywords: DNA genotyping; high-resolution melting; melt curve; real-time PCR; rs4680