title: In vitro assessment of the multifunctional bioactive potential of Alaska pollock skin collagen following simulated gastrointestinal digestion.
abstract: BACKGROUND
  Dietary mineral deficiency, hypertension and diabetes have become serious human health problems. Dietary approaches are increasingly being investigated to address these issues. Identification of food-derived biological peptides has become an important approach to control such diseases. Peptides generated from aquatic byproducts have been shown to possess biological activities.
  
  
  RESULTS
  Significantly higher copper-chelating activity was observed on simulated hydrolysis of intact collagen. The collagen hydrolysate generated in the gastric stage exhibited moderate angiotensin-converting enzyme (ACE)-inhibitory activity with an IC50 value of 2.92 ± 0.22 mg mL(-1), which significantly decreased to 0.49 ± 0.02 mg mL(-1) after intestinal digestion. The dipeptidyl peptidase (DPP) IV-inhibitory potency of the collagen hydrolysate generated directly following simulated gastrointestinal digestion (SGID) (IC50 2.59 ± 0.04 mg mL(-1)) was significantly lower than that of the collagen tryptic hydrolysate (CTH) (IC50 1.53 ± 0.01 mg mL(-1)). The antioxidant activities of collagen and CTH using the ferric-reducing antioxidant power (FRAP) assay were 0.87 ± 0.10 and 1.27 ± 0.03 µmol Trolox equivalent (TE) g(-1) respectively after SGID.
  
  
  CONCLUSIONS
  This study identifies collagen as a good and inexpensive substrate for the generation of biologically active peptides with potential applications as functional ingredients in the management of chronic illness and mineral deficiency problems.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong, 266003, China; Department of Life Sciences, University of Limerick, Limerick, Ireland.
authors.forename: Lidong
authors.initials: L
authors.lastname: Guo
authors.forename: Pádraigín A
authors.initials: PA
authors.lastname: Harnedy
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Bafang
authors.initials: B
authors.lastname: Li
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hu
authors.initials: H
authors.lastname: Hou
authors.forename: Xue
authors.initials: X
authors.lastname: Zhao
authors.forename: Richard J
authors.initials: RJ
authors.lastname: FitzGerald
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D002614:Chelating Agents / Q000378:metabolism / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism / Q000494:pharmacology*; D004044:Dietary Proteins / Q000378:metabolism / Q000494:pharmacology*; D004063:Digestion; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000378:metabolism / Q000494:pharmacology; D029941:Fish Proteins / Q000378:metabolism / Q000494:pharmacology; D048388:Gadiformes; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000378:metabolism / Q000494:pharmacology*; D017747:Seafood; D012867:Skin / Q000737:chemistry*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D002614:Chelating Agents; D004044:Dietary Proteins; D054873:Dipeptidyl-Peptidase IV Inhibitors; D029941:Fish Proteins; D010455:Peptides; D011492:Protein Hydrolysates; D003094:Collagen
keywords: chelating peptides; collagen; functional food; simulated gastrointestinal digestion