title: Plant development. Integration of growth and patterning during vascular tissue formation in Arabidopsis.
abstract: Coordination of cell division and pattern formation is central to tissue and organ development, particularly in plants where walls prevent cell migration. Auxin and cytokinin are both critical for division and patterning, but it is unknown how these hormones converge upon tissue development. We identify a genetic network that reinforces an early embryonic bias in auxin distribution to create a local, nonresponding cytokinin source within the root vascular tissue. Experimental and theoretical evidence shows that these cells act as a tissue organizer by positioning the domain of oriented cell divisions. We further demonstrate that the auxin-cytokinin interaction acts as a spatial incoherent feed-forward loop, which is essential to generate distinct hormonal response zones, thus establishing a stable pattern within a growing vascular tissue.
authors.affiliation: Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands.
authors.forename: Bert
authors.initials: B
authors.lastname: De Rybel
authors.affiliation: LifeGlimmer GmbH, Markelstrasse 38, 12163 Berlin, Germany. Albert-Ludwigs-University Freiburg, Faculty of Biology, Plant Biotechnology, Schaenzlestrasse 1, D-79104 Freiburg, Germany. Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands.
authors.forename: Milad
authors.initials: M
authors.lastname: Adibi
authors.affiliation: Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands.
authors.forename: Alice S
authors.initials: AS
authors.lastname: Breda
authors.affiliation: Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands.
authors.forename: Jos R
authors.initials: JR
authors.lastname: Wendrich
authors.affiliation: Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands.
authors.forename: Margot E
authors.initials: ME
authors.lastname: Smit
authors.affiliation: Umeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, SLU, SE-901 83 Umeå, Sweden. Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University and Institute of Experimental Botany AS CR, Šlechtitelů 11, CZ-78371 Olomouc, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Novák
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, PA 190104-6084, USA.
authors.forename: Nobutoshi
authors.initials: N
authors.lastname: Yamaguchi
authors.affiliation: Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands.
authors.forename: Saiko
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052 Gent, Belgium.
authors.forename: Gert
authors.initials: G
authors.lastname: Van Isterdael
authors.affiliation: Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands.
authors.forename: Joakim
authors.initials: J
authors.lastname: Palovaara
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands.
authors.forename: Bart
authors.initials: B
authors.lastname: Nijsse
authors.affiliation: Division of Human Nutrition, Wageningen University, Dreijenlaan 2, 6703HA Wageningen, the Netherlands. TI Food and Nutrition, 6703HA Wageningen, the Netherlands.
authors.forename: Mark V
authors.initials: MV
authors.lastname: Boekschoten
authors.affiliation: Division of Human Nutrition, Wageningen University, Dreijenlaan 2, 6703HA Wageningen, the Netherlands.
authors.forename: Guido
authors.initials: G
authors.lastname: Hooiveld
authors.affiliation: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium. Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052 Gent, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Beeckman
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, PA 190104-6084, USA.
authors.forename: Doris
authors.initials: D
authors.lastname: Wagner
authors.affiliation: Umeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, SLU, SE-901 83 Umeå, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Ljung
authors.affiliation: Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands. dolf.weijers@wur.nl christian.fleck@wur.nl.
authors.forename: Christian
authors.initials: C
authors.lastname: Fleck
authors.affiliation: Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands. dolf.weijers@wur.nl christian.fleck@wur.nl.
authors.forename: Dolf
authors.initials: D
authors.lastname: Weijers
mesh_terms: D000619:Aminohydrolases; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D019521:Body Patterning / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D009687:Nuclear Proteins / Q000235:genetics; D058526:Plant Vascular Bundle / Q000187:drug effects / Q000254:growth & development*; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D000619:Aminohydrolases; D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D016207:Cytokines; D007210:Indoleacetic Acids; D009687:Nuclear Proteins; D015534:Trans-Activators; C523529:LHW protein, Arabidopsis; C549371:TARGET OF MP 5 protein, Arabidopsis; C000589428:cytokinin riboside 5'-monophosphate phosphoribohydrolase, Arabidopsis