title: Towards the feasibility of using ultrasound to determine mechanical properties of tissues in a bioreactor.
abstract: Our ultimate goal is to non-destructively evaluate mechanical properties of tissue-engineered (TE) cartilage using ultrasound (US). We used agarose gels as surrogates for TE cartilage. Previously, we showed that mechanical properties measured using conventional methods were related to those measured using US, which suggested a way to non-destructively predict mechanical properties of samples with known volume fractions. In this study, we sought to determine whether the mechanical properties of samples, with unknown volume fractions could be predicted by US. Aggregate moduli were calculated for hydrogels as a function of SOS, based on concentration and density using a poroelastic model. The data were used to train a statistical model, which we then used to predict volume fractions and mechanical properties of unknown samples. Young's and storage moduli were measured mechanically. The statistical model generally predicted the Young's moduli in compression to within <10% of their mechanically measured value. We defined positive linear correlations between the aggregate modulus predicted from US and both the storage and Young's moduli determined from mechanical tests. Mechanical properties of hydrogels with unknown volume fractions can be predicted successfully from US measurements. This method has the potential to predict mechanical properties of TE cartilage non-destructively in a bioreactor.
authors.affiliation: Department of Mechanical & Aerospace Engineering, Case Western Reserve University, 2123 Martin Luther King Jr. Drive, Glennan Building Room 616A, Cleveland, OH, 44106, USA, jmm12@case.edu.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Mansour
authors.forename: Di-Win Marine
authors.initials: DW
authors.lastname: Gu
authors.forename: Chen-Yuan
authors.initials: CY
authors.lastname: Chung
authors.forename: Joseph
authors.initials: J
authors.lastname: Heebner
authors.forename: Jake
authors.initials: J
authors.lastname: Althans
authors.forename: Sarah
authors.initials: S
authors.lastname: Abdalian
authors.forename: Mark D
authors.initials: MD
authors.lastname: Schluchter
authors.forename: Yiying
authors.initials: Y
authors.lastname: Liu
authors.forename: Jean F
authors.initials: JF
authors.lastname: Welter
mesh_terms: D019149:Bioreactors; D002358:Cartilage, Articular / Q000502:physiology*; D055119:Elastic Modulus; D020100:Hydrogels; D015233:Models, Statistical; D012685:Sepharose; D013314:Stress, Mechanical; D023822:Tissue Engineering; D014465:Ultrasonics / Q000379:methods*
chemical_list: D020100:Hydrogels; D012685:Sepharose
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