title: Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA.
abstract: We describe a general and simple method to identify catalytically and structurally important nucleotides in functional RNAs. Our approach is based on statistical replacement of each nucleoside with a non-nucleosidic spacer (C3 linker, Δ), followed by separation of active library variants and readout of interference effects by analysis of enzymatic primer extension reactions.
authors.affiliation: Research Group Nucleic Acid Chemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. claudia.hoebartner@mpibpc.mpg.de.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wawrzyniak-Turek
authors.forename: Claudia
authors.initials: C
authors.lastname: Höbartner
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D016296:Mutagenesis; D009705:Nucleosides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D012313:RNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016337:RNA, Catalytic / Q000378:metabolism; D017423:Sequence Analysis, RNA
chemical_list: D009705:Nucleosides; D009943:Organophosphorus Compounds; D016337:RNA, Catalytic; C434331:phosphoramidite; D012313:RNA; D012321:DNA-Directed RNA Polymerases