title: Effects of Eucommia ulmoides extract on longitudinal bone growth rate in adolescent female rats.
abstract: Eucommia ulmoides is one of the popular tonic herbs for the treatment of low back pain and bone fracture and is used in Korean medicine to reinforce muscles and bones. This study was performed to investigate the effects of E. ulmoides extract on longitudinal bone growth rate, growth plate height, and the expressions of bone morphogenetic protein 2 (BMP-2) and insulin-like growth factor 1 (IGF-1) in adolescent female rats. In two groups, we administered a twice-daily dosage of E. ulmoides extract (at 30 and 100 mg/kg, respectively) per os over 4 days, and in a control group, we administered vehicle only under the same conditions. Longitudinal bone growth rate in newly synthesized bone was observed using tetracycline labeling. Chondrocyte proliferation in the growth plate was observed using cresyl violet dye. In addition, we analyzed the expressions of BMP-2 and IGF-1 using immunohistochemistry. Eucommia ulmoides extract significantly increased longitudinal bone growth rate and growth plate height in adolescent female rats. In the immunohistochemical study, E. ulmoides markedly increased BMP-2 and IGF-1 expressions in the proliferative and hypertrophic zones. In conclusion, E. ulmoides increased longitudinal bone growth rate by promoting chondrogenesis in the growth plate and the levels of BMP-2 and IGF-1. Eucommia ulmoides could be helpful for increasing bone growth in children who have growth retardation.
authors.affiliation: Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Jeong-Il
authors.initials: JI
authors.lastname: Lee
authors.forename: MiKyung
authors.initials: M
authors.lastname: Song
authors.forename: Donghun
authors.initials: D
authors.lastname: Lee
authors.forename: Jungbin
authors.initials: J
authors.lastname: Song
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Juyeon
authors.initials: J
authors.lastname: Park
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Choi
authors.forename: Hocheol
authors.initials: H
authors.lastname: Kim
mesh_terms: D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects; D031283:Eucommiaceae / Q000737:chemistry*; D005260:Female; D006132:Growth Plate / Q000187:drug effects; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D010936:Plant Extracts; C548904:insulin-like growth factor-1, rat; D007334:Insulin-Like Growth Factor I
keywords: BMP-2; Eucommia ulmoides; IGF-1; herbal extract; longitudinal bone growth rate