title: Twist expression in dentigerous cyst, odontogenic keratocyst, and ameloblastoma.
abstract: Epithelial-mesenchymal transition (EMT) is a process which is associated with a loss of intercellular adhesion, acquired mesenchymal shape, and increased motility by epithelial cells. Twist is one of the key regulators of EMT.In view of the distinct clinical behavior of odontogenic lesions, the objective of the present study was to investigate the immunohistochemical expression of Twist in these lesions. In this study, 70 formalin-fixed, paraffin-embedded tissue blocks of odontogenic lesion consisting of 16 unicystic ameloblastomas (UA), 17 solid ameloblastomas (SA), 18 odontogenic keratocysts (OKC), and 19 dentigerous cysts (DC) were reviewed using immunohistochemistry for Twist staining. In this study, Twist immunostaining was evident in all groups of the specimens except the dentigerous cyst group. Twist expression was seen in 58.8 % (10/17) of SA, 50 % (8/16) of UA, and 44.4 % (8/18) of OKCs. 23.5 % of SA, 18.8 % of UA, and 16.7 % of OKCs showed Twist expression in more than 50 % of cells. Statistical analysis showed that Twist expression levels were significantly higher in ameloblastomas (SA and UA) and OKCs than dentigerous cysts (P = 0.002). There were no significant differences between Twist expression in SAs, UAs, and OKCs (P > 0.05). The results of this study propose that the high expression rate of Twist plays a role in the pathogenesis of ameloblastomas and OKCs and might be one of the reasons for the aggressive behavior of ameloblastomas and high recurrence of OKCs and could reinforce the classification of OKC as an odontogenic tumor.
authors.affiliation: Department of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran, andisheh202003@yahoo.com.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Andisheh-Tadbir
authors.forename: Soheil
authors.initials: S
authors.lastname: Pardis
authors.forename: Pegah
authors.initials: P
authors.lastname: Ranjbaran
mesh_terms: D000564:Ameloblastoma / Q000473:pathology*; D003803:Dentigerous Cyst / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007573:Jaw Neoplasms / Q000473:pathology*; D009687:Nuclear Proteins / Q000032:analysis*; D009807:Odontogenic Cysts / Q000473:pathology*; D012189:Retrospective Studies; D051879:Twist-Related Protein 1 / Q000032:analysis*
chemical_list: D009687:Nuclear Proteins; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1
references.citation: Br J Cancer. 2007 Jan 29;96(2):314-20
references.pmid: 17211477
references.citation: Physiol Rev. 2003 Apr;83(2):337-76
references.pmid: 12663862
references.citation: Genes Chromosomes Cancer. 2007 Jul;46(7):656-60
references.pmid: 17437280
references.citation: Nucleic Acids Res. 2011 Mar;39(4):1177-86
references.pmid: 20935057
references.citation: J Cell Biochem. 1999 Dec 15;75(4):566-77
references.pmid: 10572240
references.citation: J Cell Biol. 2003 Mar 31;160(7):985-8
references.pmid: 12701595
references.citation: Cancer Sci. 2010 Feb;101(2):293-9
references.pmid: 19961486
references.citation: Mol Cancer Res. 2011 Dec;9(12):1644-57
references.pmid: 22006115
references.citation: Cell. 2004 Jun 25;117(7):927-39
references.pmid: 15210113
references.citation: J Oral Pathol Med. 2008 May;37(5):287-93
references.pmid: 18205742
references.citation: Dev Dyn. 2005 Jul;233(3):706-20
references.pmid: 15937929
references.citation: Cancer Res. 2005 Jun 15;65(12):5153-62
references.pmid: 15958559
references.citation: Gene. 2002 Apr 3;287(1-2):11-22
references.pmid: 11992718
references.citation: Rom J Morphol Embryol. 2012;53(4):975-84
references.pmid: 23303021
references.citation: Indian J Pathol Microbiol. 2012 Oct-Dec;55(4):443-9
references.pmid: 23455777
references.citation: Oncogene. 2004 Jan 15;23(2):474-82
references.pmid: 14724576
references.citation: Cancer Lett. 2006 Oct 28;242(2):258-65
references.pmid: 16412561
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Oct;108(4):565-70
references.pmid: 19699120
references.citation: Clin Oral Investig. 2011 Jun;15(3):321-8
references.pmid: 20195877
references.citation: Cancer Res. 2005 Dec 1;65(23):10801-9
references.pmid: 16322226
references.citation: Curr Opin Cell Biol. 2003 Dec;15(6):740-6
references.pmid: 14644200
references.citation: Cell. 2004 Aug 6;118(3):277-9
references.pmid: 15294153
references.citation: J Oral Pathol Med. 2014 Jan;43(1):45-52
references.pmid: 23560539
references.citation: Int J Biochem Cell Biol. 2006;38(12):2018-39
references.pmid: 16901744
references.citation: FEBS J. 2012 Jul;279(13):2393-8
references.pmid: 22540680
references.citation: Development. 2005 Jul;132(14):3151-61
references.pmid: 15983400
references.citation: Nat Rev Cancer. 2002 Jun;2(6):442-54
references.pmid: 12189386
references.citation: Clin Oral Investig. 2010 Feb;14(1):27-34
references.pmid: 19294436
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Mar;109(3):425-31
references.pmid: 20219600
references.citation: Gene. 1997 Mar 10;187(1):83-92
references.pmid: 9073070
references.citation: Cancer Res. 2004 Aug 1;64(15):5270-82
references.pmid: 15289333
references.citation: Clin Cancer Res. 2007 Aug 15;13(16):4769-76
references.pmid: 17699854
references.citation: Oncogene. 2008 Sep 18;27(42):5543-53
references.pmid: 18504427
references.citation: Am J Pathol. 2002 Nov;161(5):1881-91
references.pmid: 12414534
references.citation: J Mammary Gland Biol Neoplasia. 2010 Jun;15(2):117-34
references.pmid: 20490631