title: Response of Saccharomyces cerevisiae to the stimulation of lipopolysaccharide.
abstract: Lipopolysaccharide, known as endotoxin, can stimulate potent host immune responses through the complex of Toll-like-receptor 4 and myeloid differentiation protein 2; but its influence on Saccharomyces cerevisiae, a model organism for studying eukaryotes, is not clear. In this study, we found that lipopolysaccharide-treated S. cerevisiae cells could be stained by methylene blue, but did not die. Transcriptional profiling of the lipopolysaccharide-treated S. cerevisiae cells showed that 5745 genes were modulated: 2491 genes up-regulated and 3254 genes down-regulated. Significantly regulated genes (460 up-regulated genes and 135 down-regulated genes) in lipopolysaccharide-treated S. cerevisiae cells were analyzed on Gene Ontology, and used to establish physical protein-protein interaction network and protein phosphorylation network. Based on these analyses, most of the regulated genes in lipopolysaccharide-treated S. cerevisiae cells were related to cell wall, membrane, peroxisome and mitochondrion. Further experiments demonstrated that lipopolysaccharide stimulation caused the exposure of phosphatidylserine and the increase of mitochondrial membrane potential in S. cerevisiae cells, but levels of intracellular reactive oxygen species and metacaspase activation were not increased. This study demonstrated that lipopolysaccharide stimulation causes significant changes in S. cerevisiae cells, and the results would contribute to understand the response of eukaryotic cells to lipopolysaccharide stimulation.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; School of Biotechnology, Jiangnan University, Wuxi, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Shen
authors.affiliation: School of Biotechnology, Jiangnan University, Wuxi, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Biotechnology, Jiangnan University, Wuxi, China.
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D008070:Lipopolysaccharides / Q000276:immunology*; D060066:Protein Interaction Maps; D017382:Reactive Oxygen Species / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000276:immunology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins
references.citation: Microbiology (Reading). 2004 Jan;150(Pt 1):109-115
references.pmid: 14702403
references.citation: J Cell Biol. 2005 Jan 17;168(2):257-69
references.pmid: 15657396
references.citation: Mol Cell. 2001 Oct;8(4):921-30
references.pmid: 11684026
references.citation: Autophagy. 2009 Aug;5(6):847-9
references.pmid: 19458476
references.citation: Cell Death Differ. 2010 May;17(5):763-73
references.pmid: 20075938
references.citation: J Gastroenterol Hepatol. 1994 Sep-Oct;9(5):512-3
references.pmid: 7530062
references.citation: J Cell Biol. 2006 Nov 20;175(4):521-5
references.pmid: 17101700
references.citation: J Cell Biol. 1997 Nov 3;139(3):729-34
references.pmid: 9348289
references.citation: Nat Rev Immunol. 2003 Feb;3(2):169-76
references.pmid: 12563300
references.citation: Subcell Biochem. 2010;53:3-25
references.pmid: 20593260
references.citation: Curr Opin Microbiol. 2004 Dec;7(6):655-60
references.pmid: 15556039
references.citation: Nat Commun. 2012;3:1112
references.pmid: 23047670
references.citation: Pediatr Res. 2006 Jul;60(1):24-9
references.pmid: 16690953
references.citation: Bioinformatics. 2009 Jan 1;25(1):137-8
references.pmid: 18812364
references.citation: FEMS Yeast Res. 2007 May;7(3):404-12
references.pmid: 17233764
references.citation: Nat Cell Biol. 2011 May;13(5):589-98
references.pmid: 21478857
references.citation: J Appl Microbiol. 2013 Feb;114(2):287-98
references.pmid: 23020588
references.citation: J Biol Chem. 2006 Aug 25;281(34):24687-94
references.pmid: 16790423
references.citation: Bioinformatics. 2009 Nov 1;25(21):2857-9
references.pmid: 19729372
references.citation: Cell Death Differ. 2012 Jan;19(1):107-20
references.pmid: 21760595
references.citation: J Microbiol Biotechnol. 2007 Jul;17(7):1120-6
references.pmid: 18051322
references.citation: Biochemistry. 2009 Feb 17;48(6):1162-72
references.pmid: 19166327
references.citation: Prog Lipid Res. 2010 Apr;49(2):97-107
references.pmid: 19815028
references.citation: Science. 1990 Sep 21;249(4975):1431-3
references.pmid: 1698311
references.citation: Cytometry. 1998 Nov 1;33(3):333-9
references.pmid: 9822344
references.citation: Free Radic Biol Med. 2006 Dec 1;41(11):1655-61
references.pmid: 17145553
references.citation: World J Microbiol Biotechnol. 1990 Sep;6(3):346-7
references.pmid: 24430080
references.citation: Microbiol Mol Biol Rev. 2001 Sep;65(3):463-79, table of contents
references.pmid: 11528006
references.citation: Genome Res. 2003 Nov;13(11):2498-504
references.pmid: 14597658
references.citation: PLoS Comput Biol. 2007 Apr 20;3(4):e59
references.pmid: 17447836
references.citation: World J Microbiol Biotechnol. 2014 Jan;30(1):125-33
references.pmid: 23861041
references.citation: Cell Biol Int. 2000;24(10):757-60
references.pmid: 11023655
references.citation: BMC Complement Altern Med. 2012 Oct 24;12:194
references.pmid: 23092287
references.citation: Mol Cell. 2002 Sep;10(3):457-68
references.pmid: 12408816
references.citation: Stain Technol. 1961 Sep;36:329-30
references.pmid: 13924214
references.citation: Methods Enzymol. 1991;194:710-7
references.pmid: 2005818
references.citation: Bioinformatics. 2005 Aug 15;21(16):3448-9
references.pmid: 15972284
references.citation: Mol Cell. 2002 Apr;9(4):911-7
references.pmid: 11983181
references.citation: Annu Rev Biochem. 2002;71:635-700
references.pmid: 12045108
references.citation: Mol Med Today. 1999 Mar;5(3):123-32
references.pmid: 10203736
references.citation: Mol Microbiol. 2005 Nov;58(3):824-34
references.pmid: 16238630
references.citation: Mar Drugs. 2013 Jan 31;11(2):377-86
references.pmid: 23434833
references.citation: Genetics. 2012 Apr;190(4):1157-95
references.pmid: 22209905
references.citation: Nature. 2005 Dec 1;438(7068):679-84
references.pmid: 16319894
references.citation: FEMS Yeast Res. 2008 Jun;8(4):531-9
references.pmid: 18452540
references.citation: BMC Bioinformatics. 2003 Jan 13;4:2
references.pmid: 12525261
references.citation: Microbiology (Reading). 2001 Dec;147(Pt 12):3335-43
references.pmid: 11739765
references.citation: Autophagy. 2012 May 1;8(5):731-8
references.pmid: 22361650