title: Isolation and characterization of bifidobacteria from ovine cheese.
abstract: Animal products are one of the niches of bifidobacteria, a fact probably attributable to secondary contamination. In this study, 2 species of the genus Bifidobacterium were isolated by culture-dependent methods from ovine cheeses that were made from unpasteurized milk without addition of starter cultures. The isolates were identified as Bifidobacterium crudilactis and Bifidobacterium animalis subsp. lactis using matrix-assisted laser desorption/ionization time-of-flight analysis and sequencing of phylogenetic markers (16S rRNA, hsp60, and fusA).
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague 6-Suchdol 165 21, Czech Republic. Electronic address: bunesova@af.czu.cz.
authors.forename: Vera
authors.initials: V
authors.lastname: Bunesova
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague 6-Suchdol 165 21, Czech Republic; Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4-Krč 14 200, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Killer
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague 6-Suchdol 165 21, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Vlkova
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague 6-Suchdol 165 21, Czech Republic.
authors.forename: Sarka
authors.initials: S
authors.lastname: Musilova
authors.affiliation: Dairy Research Institute, Dlhá 95, 010 01 Žilina, Slovakia.
authors.forename: Martin
authors.initials: M
authors.lastname: Tomaska
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague 6-Suchdol 165 21, Czech Republic.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Rada
authors.affiliation: Institute of Animal Physiology of Slovak Academy of Sciences, Soltesovej 4, 040 01 Kosice, Slovakia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Kmet
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002611:Cheese / Q000382:microbiology*; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D034561:Sheep, Domestic
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Bifidobacterium sp.; Cultivation; MALDI-TOF; Ovine cheese; Sequencing