title: Myokine IL-15 regulates the crosstalk of co-cultured porcine skeletal muscle satellite cells and preadipocytes.
abstract: The present study was carried out to preliminarily reveal the underlying mechanisms of the co-culture system between porcine muscle satellite cells (SCs) and stromal-vascular cells (SVs). The two cell types were co-cultured to assess both proliferation and differentiation. Desmin and Pref-1 immunofluorescence staining technique were taken to identify the two types of isolated cells. The expression of specific marker genes Myogenin was up-regulated in SCs (P < 0.05) and the differentiation of SCs could be promoted when co-cultured with preadipocytes compared with the single-cultured control, while expression of c/EBPβ in SVs was down-regulated (P < 0.05) and the differentiation of preadipocytes could be inhibited. Furthermore, secretion of myokine IL-15 was markedly increased, as well as its gene and protein expression levels in co-culture supernatants. However, the secretion of adipokine leptin was significantly decreased. These findings demonstrate that myokines like IL-15 could facilitate the SCs' differentiation while inhibit the SVs differentiation, and act as an important regulator of co-culture between muscle cells and adipocytes.
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, No. 644 Yuanda Road, Furong District, Changsha Hunan, 410125, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.forename: Fengna
authors.initials: F
authors.lastname: Li
authors.forename: Binbin
authors.initials: B
authors.lastname: Lin
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Kong
authors.forename: Yulong
authors.initials: Y
authors.lastname: Tang
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D018920:Coculture Techniques; D017931:DNA Primers / Q000235:genetics; D005455:Fluorescent Antibody Technique; D019409:Interleukin-15 / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism; D018008:Myogenin / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020239:Receptor Cross-Talk / Q000502:physiology*; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology*; D017154:Stromal Cells / Q000502:physiology*; D013552:Swine
chemical_list: D017931:DNA Primers; D019409:Interleukin-15; D020738:Leptin; D018008:Myogenin
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