title: RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database.
abstract: In this study, we selected 181 nematode glycogenes that are orthologous to human glycogenes and examined their RNAi phenotypes. The results are deposited in the Caenorhabditis elegans Glycogene Database (CGGDB) at AIST, Tsukuba, Japan. The most prominent RNAi phenotypes observed are disruptions of cell cycle progression in germline mitosis/meiosis and in early embryonic cell mitosis. Along with the previously reported roles of chondroitin proteoglycans, glycosphingolipids and GPI-anchored proteins in cell cycle progression, we show for the first time that the inhibition of the functions of N-glycan synthesis genes (cytoplasmic alg genes) resulted in abnormal germline formation, ER stress and small body size phenotypes. The results provide additional information on the roles of glycoconjugates in the cell cycle progression mechanisms of germline and embryonic cells.
authors.affiliation: Graduate School of Systems Life Sciences, and.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Akiyoshi
authors.affiliation: Department of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Kazuko H
authors.initials: KH
authors.lastname: Nomura
authors.affiliation: Department of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan.
authors.forename: Katsufumi
authors.initials: K
authors.lastname: Dejima
authors.affiliation: Graduate School of Systems Life Sciences, and Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Murata
authors.affiliation: Graduate School of Systems Life Sciences, and.
authors.forename: Ayako
authors.initials: A
authors.lastname: Matsuda
authors.affiliation: Department of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Nanako
authors.initials: N
authors.lastname: Kanaki
authors.affiliation: Department of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Takaki
authors.affiliation: Department of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mihara
authors.affiliation: Graduate School of Systems Life Sciences, and Department of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nagaishi
authors.affiliation: Department of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Furukawa
authors.affiliation: Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan.
authors.forename: Keiko-Gengyo
authors.initials: KG
authors.lastname: Ando
authors.affiliation: Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan.
authors.forename: Sawako
authors.initials: S
authors.lastname: Yoshina
authors.affiliation: Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Mitani
authors.affiliation: Glycomedicine Technology Research Center (GTRC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Togayachi
authors.affiliation: Glycomedicine Technology Research Center (GTRC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Glycomedicine Technology Research Center (GTRC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Japan.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Shikanai
authors.affiliation: Glycomedicine Technology Research Center (GTRC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
authors.affiliation: Department of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan knomuscb@kyushu-u.org.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nomura
mesh_terms: D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002240:Carbohydrate Sequence; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030541:Databases, Genetic; D059865:Endoplasmic Reticulum Stress / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D005838:Genotype; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D006028:Glycosphingolipids / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008540:Meiosis / Q000235:genetics; D008938:Mitosis / Q000235:genetics; D008969:Molecular Sequence Data; D010641:Phenotype; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D006001:Glycoconjugates; D006025:Glycosaminoglycans; D006028:Glycosphingolipids; D034741:RNA, Small Interfering
keywords: RNAi; alg genes; germline cell division; glycogene; oligosaccharyltransferase
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