title: Inhibition of key markers linked with spermatogenesis and cellular ATP by subchronic exposure to leachate in a rat model.
abstract: Few or no studies have measured the toxic effects of subchronic exposure to leachate using key markers linked with spermatogenesis and cellular adenosine triphosphate (ATP) in an experimental rat model. This study was undertaken to evaluate the toxic effects of leachate obtained from the Elewi Odo municipal battery-recycling site (EOMABRL) on male reproductive function using testicular hormones and biomarker of cellular ATP. EOMABRL was administered at 0, 20, 40, 60, 80, and 100% concentrations to adult male rats for 60 days. After exposure, serum was collected for hormonal biochemistry assays, and testes were collected to determine the activity of xanthine oxidase (XO) and lactate dehydrogenase (LDH). Exposure of animals to EOMABRL resulted in a 519.7, 285.7, 569.1, 606.1, and 1,793.2% increase in XO activity with a sequential decrease in LDH activity (marker of cellular ATP) by 44.1, 55.9, 61.4, 69.3, and 89.7%, respectively, compared with the control. Furthermore, EOMABRL caused a significant inhibitory effect on serum luteinizing hormone, follicle-stimulating hormone, and testosterone levels. We conclude that some possible mechanisms by which EOMABRL elicits toxicity in male rat testes could be through inhibition of LDH activity and depletion of serum hormone levels.
authors.affiliation: Functional Foods, Nutraceuticals and Phytomedicine Research Laboratory, Department of Biochemistry, Federal University of Technology, P.M.B., 704, Akure, 340001, Nigeria, akintundejacob@yahoo.com.
authors.forename: J K
authors.initials: JK
authors.lastname: Akintunde
authors.forename: G
authors.initials: G
authors.lastname: Oboh
authors.forename: A A
authors.initials: AA
authors.lastname: Akindahunsi
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D051381:Rats; D013091:Spermatogenesis / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000187:drug effects / Q000378:metabolism
chemical_list: D015415:Biomarkers; D000255:Adenosine Triphosphate; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D007770:L-Lactate Dehydrogenase