title: Antibody recognition of Plasmodium falciparum infected red blood cells by symptomatic and asymptomatic individuals in the Brazilian Amazon.
abstract: In the Amazon Region, there is a virtual absence of severe malaria and few fatal cases of naturally occurring Plasmodium falciparum infections; this presents an intriguing and underexplored area of research. In addition to the rapid access of infected persons to effective treatment, one cause of this phenomenon might be the recognition of cytoadherent variant proteins on the infected red blood cell (IRBC) surface, including the var gene encoded P. falciparum erythrocyte membrane protein 1. In order to establish a link between cytoadherence, IRBC surface antibody recognition and the presence or absence of malaria symptoms, we phenotype-selected four Amazonian P. falciparum isolates and the laboratory strain 3D7 for their cytoadherence to CD36 and ICAM1 expressed on CHO cells. We then mapped the dominantly expressed var transcripts and tested whether antibodies from symptomatic or asymptomatic infections showed a differential recognition of the IRBC surface. As controls, the 3D7 lineages expressing severe disease-associated phenotypes were used. We showed that there was no profound difference between the frequency and intensity of antibody recognition of the IRBC-exposed P. falciparum proteins in symptomatic vs. asymptomatic infections. The 3D7 lineages, which expressed severe malaria-associated phenotypes, were strongly recognised by most, but not all plasmas, meaning that the recognition of these phenotypes is frequent in asymptomatic carriers, but is not necessarily a prerequisite to staying free of symptoms.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Alessandra Sampaio Bassi
authors.initials: AS
authors.lastname: Fratus
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Fernanda Janku
authors.initials: FJ
authors.lastname: Cabral
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Wesley Luzetti
authors.initials: WL
authors.lastname: Fotoran
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Márcia Melo
authors.initials: MM
authors.lastname: Medeiros
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Bianca Cechetto
authors.initials: BC
authors.lastname: Carlos
authors.affiliation: Instituto de Pesquisas em Patologias Tropicais, Porto Velho, RO, Brasil.
authors.forename: Rosimeire dalla
authors.initials: Rd
authors.lastname: Martha
authors.affiliation: Instituto de Pesquisas em Patologias Tropicais, Porto Velho, RO, Brasil.
authors.forename: Luiz Hildebrando Pereira
authors.initials: LH
authors.lastname: da Silva
authors.affiliation: Departamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors.forename: Stefanie Costa Pinto
authors.initials: SC
authors.lastname: Lopes
authors.affiliation: Departamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors.forename: Fabio Trindade Maranhão
authors.initials: FT
authors.lastname: Costa
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wunderlich
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D058345:Asymptomatic Infections; D018955:CD36 Antigens / Q000276:immunology*; D016466:CHO Cells; D002448:Cell Adhesion / Q000235:genetics / Q000276:immunology; D003412:Cricetulus; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D016778:Malaria, Falciparum / Q000276:immunology* / Q000469:parasitology; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000913:Antibodies, Protozoan; D018955:CD36 Antigens; D015800:Protozoan Proteins; C085731:erythrocyte membrane protein 1, Plasmodium falciparum
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