title: Immunohistochemical localization of WT1 in epithelial salivary tumors.
abstract: The subcellular localization of WT1 is controversial and has received little attention in the epithelial tumors of salivary glands. Paraffin-embedded, surgical specimens from 80 salivary tumors were investigated by immunohistochemistry using a monoclonal, anti-WT1 antibody (6F-H2, Dako). Immunostaining was seen in 14/14 pleomorphic adenomas (PAs), 6/6 myoepitheliomas, 4/4 basal cell adenomas, 4/4 canalicular adenomas, 0/7 Warthin tumors, 0/1 oncocytoma, 1/6 acinic cell carcinomas (Cas), 0/11 mucoepidemoid Cas, 1/11 adenoid cystic Cas, 11/12 polymorphous low-grade adenocarcinomas (PLGAs), 1/2 Ca ex PA, 0/1 salivary duct Ca and 0/1 clear cell adenocarcinoma. Stained-cell subpopulations up to 90% were not uncommon in the benign tumors. Up to 80% of cells in PLGA could be stained. Staining was weak to intense and confined to the cytoplasm of preferentially non-luminal or adjacent to stroma cells. One adenoid cystic Ca showed nuclear staining. The results suggest that WT1 is often highly expressed in benign non-oncocytic salivary tumors whereas the malignant tumors show decreased expression, the exception being PLGA. The expression is usually cytoplasmic and associated with non-luminal cells. PLGA immunoreactivities could be useful in histological differential diagnosis.
authors.affiliation: School of Dentistry, University of Liverpool, Liverpool L3 5PS, United Kingdom.
authors.forename: Ross
authors.initials: R
authors.lastname: Leader
authors.affiliation: Cellular Pathology, Aintree University Hospital, Liverpool L9 7AL, United Kingdom.
authors.forename: Ranjeev Kaur
authors.initials: RK
authors.lastname: Deol-Poonia
authors.affiliation: Cellular Pathology, Aintree University Hospital, Liverpool L9 7AL, United Kingdom.
authors.forename: Jon
authors.initials: J
authors.lastname: Sheard
authors.affiliation: Cellular Pathology, Aintree University Hospital, Liverpool L9 7AL, United Kingdom; Oral Pathology, School of Dentistry, University of Liverpool, Liverpool L3 5PS, United Kingdom. Electronic address: A.Triantafyllou@liverpool.ac.uk.
authors.forename: Asterios
authors.initials: A
authors.lastname: Triantafyllou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000912:Antibodies, Neoplasm / Q000276:immunology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism* / Q000473:pathology; D018987:Salivary Ducts / Q000473:pathology; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D012469:Salivary Glands / Q000473:pathology; D025721:WT1 Proteins / Q000276:immunology / Q000378:metabolism*
chemical_list: D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D025721:WT1 Proteins; C583751:WT1 protein, human
keywords: Immunohistochemistry; Salivary gland tumors; Salivary glands; Wilms tumor 1 protein