title: Upregulation of miR-513b inhibits cell proliferation, migration, and promotes apoptosis by targeting high mobility group-box 3 protein in gastric cancer.
abstract: The high mobility group-box 3 (HMGB3) protein belongs to the high mobility group box (HMG-box) subfamily, and recent studies have shown that HMGB3 is an oncogene for leukemia. HMGB3 is also expressed at a high level in the progression phase of breast and gastric cancer (GC). Using bioinformatic analyses, we found that HMGB3 is a potential target for miR-513b. However, the pathophysiological role of miR-513b and its relevance to the growth and development of GC have yet to be investigated. This study focuses on whether miR-513b acts as a tumor suppressor in GC. Compared with non-malignant adjacent tissues samples, qRT-PCR data showed significant downregulation of miR-513b in 74 GC tissue samples (P < 0.01). Furthermore, western blotting revealed that HMGB3 protein was overexpressed in tumor samples relative to matched, non-malignant adjacent tissues. Western blotting and qRT-PCR results showed that high expression of HMGB3 and low expression of miR-513b were both significantly associated with primary tumors, lymph node metastases, and the clinical stage (P < 0.01). MiR-513b was shown to not only inhibit the proliferation and migration of gastric cancer cells (MKN45 and SGC7901) in the CCK-8 and transwell assays, but also to promote cell apoptosis in a flow-cytometric apoptosis assay. In western blot and luciferase assays, HMGB3 was identified as a major target of miR-513b. Moreover, we also found that the expression of HMGB3 lacking in 3' UTR could abrogate the anti-migration and pro-apoptosis function of miR-513b. These findings suggest the importance of miR-513b targeting of HMGB3 in the regulation of growth, migration and apoptosis of GC, improve our understanding of the mechanisms of GC pathogenesis, and may promote the development of novel targeted therapies.
authors.affiliation: Department of Basic Medical Sciences, Luohe Medical College, Luohe, Henan, 462002, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Chen
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
authors.forename: Fuqing
authors.initials: F
authors.lastname: Wang
authors.forename: Fenglan
authors.initials: F
authors.lastname: Gao
authors.forename: Hailan
authors.initials: H
authors.lastname: Luo
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Du
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Chen
authors.forename: Changgui
authors.initials: C
authors.lastname: Xue
authors.forename: Ziming
authors.initials: Z
authors.lastname: Dong
authors.forename: Guohua
authors.initials: G
authors.lastname: Song
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D025765:HMGB3 Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D025765:HMGB3 Protein; C546275:MIRN513A1 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
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