title: Metals and (metallo)proteins identification in vitreous humor focusing on post-mortem biochemistry.
abstract: This work describes the evaluation of metals and (metallo)proteins in vitreous humor samples and their correlations with some biological aspects in different post-mortem intervals (1-7 days), taking into account both decomposing and non-decomposing bodies. After qualitative evaluation of the samples involving 26 elements, representative metal ions (Fe, Mg and Mo) are determined by inductively coupled plasma mass spectrometry after using mini-vial decomposition system for sample preparation. A significant trend for Fe is found with post-mortem time for decomposing bodies because of a significant increase of iron concentration when comparing samples from bodies presenting 3 and 7 days post-mortem interval. An important clue to elucidate the role of metals is the coupling of liquid chromatography with inductively coupled plasma mass spectrometry for identification of metals linked to proteins, as well as mass spectrometry for the identification of those proteins involved in the post-mortem interval.
authors.affiliation: Department of Clinical Pathology, School of Medical Sciences. State University of Campinas - UNICAMP, 181 Alexander Fleming St, 13083-881 Campinas, SP, Brazil. nelci@fcm.unicamp.br.
authors.forename: Júlio César
authors.initials: JC
authors.lastname: Santos Júnior
authors.forename: Pedro Carlos
authors.initials: PC
authors.lastname: Mollo Filho
authors.forename: Ruggero Bernardo
authors.initials: RB
authors.lastname: Felice Guidugli
authors.forename: Marcos Nogueira
authors.initials: MN
authors.lastname: Eberlin
authors.forename: Gustavo de Souza
authors.initials: Gde S
authors.lastname: Pessôa
authors.forename: Elidiane Gomes
authors.initials: EG
authors.lastname: da Silva
authors.forename: Marco Aurélio Zezzi
authors.initials: MA
authors.lastname: Arruda
authors.forename: Nelci Fenalti
authors.initials: NF
authors.lastname: Höehr
mesh_terms: D000595:Amino Acid Sequence; D001344:Autopsy / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008667:Metalloproteins / Q000032:analysis*; D008670:Metals / Q000032:analysis* / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D014822:Vitreous Body / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008667:Metalloproteins; D008670:Metals; D011506:Proteins