title: Prevalence of hepatitis C virus genotypes in district bannu, khyber pakhtunkhwa, pakistan.
abstract: Determination of an individual's hepatitis C virus (HCV) genotypes prior to antiviral therapy has become increasingly important for the clinical management and prognosis of HCV infection. Therefore, this study was conducted to investigate the prevalence of HCV genotypes in HCV infected patients of district Bannu in Khyber Pakhtunkhwa region of Pakistan. Serum samples of 117 seropositive patients were screened for HCV-RNA by using reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) and then PCR positive samples were subjected to HCV genotyping. Out of 117 seropositive samples, 110 samples were found positive by PCR analysis. Genotype 3a was the most prevalent one detected in 38% of patients, followed by genotype 3b in 21% of patients, and then genotype 2a in 12% of patients. However 21% of HCV-PCR positive samples could not be genotyped by method used in this study. Genotype 3a was the most prevalent genotype in patients of all age groups and its prevalence was found high among patients with increasing age (>34 years). Moreover, genotypes 3a and 3b were found to be the most prevalent genotypes in patients with history of shaving by barbers, receiving multiple injections, and dental procedures. In conclusion there is need of further investigation of genotypes of HCV by using more sensitive assays and considering large sample size in district Bannu.
authors.affiliation: Department of Microbiology, Kohat University of Science and Technology (KUST), Khyber Pakhtunkhwa, Kohat 26000, Pakistan.
authors.forename: Shamim
authors.initials: S
authors.lastname: Saleha
authors.affiliation: Department of Microbiology, Kohat University of Science and Technology (KUST), Khyber Pakhtunkhwa, Kohat 26000, Pakistan.
authors.forename: Anwar
authors.initials: A
authors.lastname: Kamal
authors.affiliation: Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology (KUST), Khyber Pakhtunkhwa, Kohat 26000, Pakistan.
authors.forename: Farman
authors.initials: F
authors.lastname: Ullah
authors.affiliation: Department of Microbiology, Kohat University of Science and Technology (KUST), Khyber Pakhtunkhwa, Kohat 26000, Pakistan.
authors.forename: Nasar
authors.identifier: 0000-0002-2587-1721
authors.initials: N
authors.lastname: Khan
authors.affiliation: Department of Microbiology, Kohat University of Science and Technology (KUST), Khyber Pakhtunkhwa, Kohat 26000, Pakistan.
authors.forename: Asif
authors.initials: A
authors.lastname: Mahmood
authors.affiliation: Department of Zoology, Kohat University of Science and Technology (KUST), Khyber Pakhtunkhwa, Kohat 26000, Pakistan.
authors.forename: Sanaullah
authors.identifier: 0000-0002-7034-2570
authors.initials: S
authors.lastname: Khan
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