title: Effect of hydroxyapatite nanoparticles on osmotic responses of pig iliac endothelial cells.
abstract: In order to fully explore the potential applications of nanoparticles in biopreservation, it is necessary to study the effect of nanoparticles on cell membrane permeabilities. The aim of this study is therefore to comparatively evaluate the osmotic responses of pig iliac endothelial cells in the absence and presence of commercially available hydroxyapatite nanoparticles. The results indicate that, after the introduction of 0.0 1 wt% hydroxyapatite nanoparticles, the dependence of cell membrane hydraulic conductivity (Lp) on temperature still obeys the Arrhenius relationship, while the reference value of the hydraulic conductivity of the cell membrane at 273.15K (Lpg) and the activation energy for water transport across cell membrane (ELp) change from 0.77 × 10(-14)m/Pa/s and 15.65 kJ/mol to 0.65 × 10(-14)m/Pa/s and 26.14 kJ/mol. That is to say, the reference value of the hydraulic conductivity of the cell membrane has been slightly decreased while the activation energy for water transport across cell membrane has been greatly enhanced, and thus it implies that the hydraulic conductivity of cell membrane are more sensitive to temperature in the presence of nanoparticles. These findings are of potential significance to the optimization of nanoparticles-aided cryopreservation.
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, Anhui, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Yue
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, Anhui, China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei 230027, Anhui, China. Electronic address: zhaog@ustc.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, Anhui, China.
authors.forename: Jingru
authors.initials: J
authors.lastname: Yi
authors.affiliation: School of Mechanical and Automotive Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address: gawtsai@126.com.
authors.forename: Cai
authors.initials: C
authors.lastname: Gao
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, Anhui, China.
authors.forename: Lingxiao
authors.initials: L
authors.lastname: Shen
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, Anhui, China.
authors.forename: Yuntian
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, Anhui, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, Anhui, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D048429:Cell Size; D017886:Durapatite / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D004867:Equipment Design; D005615:Freezing; D053758:Nanoparticles / Q000378:metabolism*; D009995:Osmosis; D009997:Osmotic Pressure; D010477:Perfusion / Q000295:instrumentation; D013552:Swine; D013816:Thermodynamics; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D017886:Durapatite
keywords: Activation energy; Hydraulic conductivity; Hydroxyapatite nanoparticles; Microperfusion chamber; Osmotically inactive cell volume