title: Interaction between abscisic acid receptor PYL3 and protein phosphatase type 2C in response to ABA signaling in maize.
abstract: Abscisic acid (ABA) is a ubiquitous hormone that regulates plant growth, development and responses to environmental stresses. In recent researches, pyrabactin resistance 1-like protein (PYL) and protein phosphatase type 2C (PP2C) were identified as the direct receptor and the second component of ABA signaling pathway, respectively. However, a lot of PYL and PP2C members were found in Arabidopsis and several other plants. Some of them were found not to be involved in ABA signaling. Because of the complex diversity of the genome, few documents have been available on the molecular details of the ABA signal perception system in maize. In the present study, we conducted bioinformatics analysis to find out the candidates (ZmPYL3 and ZmPP2C16) of the PYL and PP2C members most probably involved in ABA signaling in maize, cloned their encoding genes (ZmPYL3 and ZmPP2C16), verified the interaction between these two proteins in response to exogenous ABA induction by yeast two-hybrid assay and bimolecular fluorescence complementation, and investigated the expression patterns of these two genes under the induction of exogenous ABA by real-time fluorescence quantitative PCR. The results indicated that the ZmPYL3 and ZmPP2C16 proteins interacted in vitro and in vivo in response to the induction of exogenous ABA. The downregulated expression of the ZmPYL3 gene and the upregulated expression of the ZmPP2C16 gene are responsive to the induction of exogenous ABA. The ZmPYL3 and ZmPP2C16 proteins are the most probable members of the receptors and the second components of ABA signaling pathway, respectively.
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
authors.forename: Ying-Ge
authors.initials: YG
authors.lastname: Wang
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
authors.forename: Hao-Qiang
authors.initials: HQ
authors.lastname: Yu
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
authors.forename: Cong-Xian
authors.initials: CX
authors.lastname: Lai
authors.affiliation: Agronomy Faculty, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: She
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China. Electronic address: aumdyms@sicau.edu.cn.
authors.forename: Wan-Chen
authors.initials: WC
authors.lastname: Li
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China. Electronic address: ffl@sicau.edu.cn.
authors.forename: Feng-Ling
authors.initials: FL
authors.lastname: Fu
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D003001:Cloning, Molecular; D019295:Computational Biology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D000071636:Protein Phosphatase 2C; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D003313:Zea mays / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C
keywords: Abscisic acid; Maize; Protein phosphatase type 2C; Receptor; Signaling pathway