title: RNAi mediated gene silencing against betasatellite associated with Croton yellow vein mosaic begomovirus.
abstract: Plant viruses encode suppressors of posttranscriptional gene silencing, an adaptive antiviral defense responses that confines virus infection. Previously, we identified single-stranded DNA satellite (also known as DNA-β) of ~1,350 nucleotides in length associated with Croton yellow vein mosaic begomovirus (CYVMV) in croton plants. The expression of genes from DNA-β requires the begomovirus for packaged, replication, insect transmission and movement in plants. The present study demonstrates the effect of the βC1 gene on the silencing pathway as analysed by using both transgenic systems and transient Agrobacterium tumefaciens based delivery. Plants that carry an intron-hairpin construct covering the βC1 gene accumulated cognate small-interfering RNAs and remained symptom-free after exposure to CYVMV and its satellite. These results suggest that βC1 interferes with silencing mechanism.
authors.affiliation: Department of Science, Faculty of Arts, Science and Commerce, Mody Institute of Technology and Science, Lakshmangarh, Sikar, 332311, India.
authors.forename: Anurag Kumar
authors.initials: AK
authors.lastname: Sahu
authors.forename: Avinash
authors.initials: A
authors.lastname: Marwal
authors.forename: Chitra
authors.initials: C
authors.lastname: Nehra
authors.forename: Devendra Kumar
authors.initials: DK
authors.lastname: Choudhary
authors.forename: Pradeep
authors.initials: P
authors.lastname: Sharma
authors.forename: Rajarshi Kumar
authors.initials: RK
authors.lastname: Gaur
mesh_terms: D016960:Agrobacterium tumefaciens; D053839:Begomovirus / Q000235:genetics* / Q000276:immunology; D015152:Blotting, Northern; D015139:Blotting, Southern; D029802:Croton / Q000235:genetics* / Q000276:immunology* / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D034622:RNA Interference / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D017931:DNA Primers; D004276:DNA, Satellite; D034741:RNA, Small Interfering
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