title: RNA aptamers for targeting mitochondria using a mitochondria-based SELEX method.
abstract: The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2'-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1.
authors.affiliation: Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Tawaraya
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hyodo
authors.forename: Mst Naznin
authors.initials: MN
authors.lastname: Ara
authors.forename: Yuma
authors.initials: Y
authors.lastname: Yamada
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015723:Gene Library; D008024:Ligands; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D017208:Rats, Wistar; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D008024:Ligands