title: The VEGF +405 G>C 5' untranslated region polymorphism and risk of PCOS: a study in the South Indian Women.
abstract: OBJECTIVE
  The aim of this study was to investigate the association between two common single nucleotide polymorphisms (SNPs) in the vascular endothelial growth factor (VEGF) gene (-460C/T and +405G/C) and polycystic ovary syndrome (PCOS) risk in south Indian women.
  
  
  METHODS
  This study involves clinically confirmed PCOS patients (n = 126) and non-PCOS controls (n = 130) of south Indian origin (Dravidian linguistic group). Genotyping of the VEGF gene -460C/T and +405G/C SNPs were performed by PCR and sequencing analysis. Haplotype frequencies for multiple loci and the standardized disequilibrium coefficient (D') for pairwise linkage disequilibrium (LD) were assessed by Haploview Software.
  
  
  RESULTS
  The frequencies of +405G/G genotype (P = 0.03) and +405G alleles (P = 0.006) were significantly higher in patients compared to controls. Whereas the genotype and allele frequencies of -460C/T SNP were not significantly different between patients and controls. In addition, LD analysis revealed no significant difference between patients and controls.
  
  
  CONCLUSIONS
  Our findings suggest that the VEGF +405G/C polymorphism may constitute an inheritable risk factor for PCOS in south Indian women.
authors.affiliation: Department of Biochemistry, Osmania University, 500 007, Hyderabad, India, renapraveen@gmail.com.
authors.forename: Praveen
authors.initials: P
authors.lastname: Guruvaiah
authors.forename: Suresh
authors.initials: S
authors.lastname: Govatati
authors.forename: Tumu Venkat
authors.initials: TV
authors.lastname: Reddy
authors.forename: Dakshayani
authors.initials: D
authors.lastname: Lomada
authors.forename: Mamata
authors.initials: M
authors.lastname: Deenadayal
authors.forename: Sisinthy
authors.initials: S
authors.lastname: Shivaji
authors.forename: Manjula
authors.initials: M
authors.lastname: Bhanoori
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012306:Risk; D012307:Risk Factors; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
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