title: Decoration of silicon nanostructures with copper particles for simultaneous selective capture and mass spectrometry detection of His-tagged model peptide.
abstract: We present in this work a simple and fast preparation method of a new affinity surface-assisted laser/desorption ionization mass spectrometry (SALDI-MS) substrate based on silicon nanostructures decorated with copper particles. The silicon nanostructures were fabricated by the metal-assisted chemical etching (MACE) method. Then, superhydrophilic areas surrounded by superhydrophobic regions were formed through hydrosilylation reaction of 1-octadecene, followed by local degradation of the octadecyl layer. After that, copper particles were deposited in the hydrophilic areas by using the electroless method. We have demonstrated that these surfaces were able to perform high selective capture of model His-tag peptide even in a complex mixture such as serum solution. Then, the captured peptide was detected by mass spectrometry at a femtomolar level without the need of organic matrix.
authors.affiliation: Institut de Recherche Interdisciplinaire (IRI, CNRS-USR 3078), Université Lille1, Parc scientifique de la Haute Borne, 50 Avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France. yannick.coffinier@iri.univ-lille1.fr.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Coffinier
authors.forename: Ievgen
authors.initials: I
authors.lastname: Kurylo
authors.forename: Hervé
authors.initials: H
authors.lastname: Drobecq
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
authors.forename: Oleg
authors.initials: O
authors.lastname: Melnyk
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Zaitsev
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
mesh_terms: D000475:Alkenes / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry; D010455:Peptides / Q000032:analysis*; D012825:Silicon / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000475:Alkenes; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D010455:Peptides; D006639:Histidine; D003300:Copper; C109760:1-octadecene; D012825:Silicon