title: The detergent fraction is effective in the detection of IgG anti-Strongyloides stercoralis in serum samples from immunocompromised individuals.
abstract: Human strongyloidiasis is an intestinal helminthiasis that can be fatal particularly in cases of immunosuppression. The aim of this study is to assess the diagnostic accuracy of the detergent fraction (D), purified from total saline extract (SE) of Strongyloides venezuelensis, in the detection of anti-Strongyloides stercoralis IgG antibodies in serum samples from individuals coming from endemic areas for strongyloidiasis and presenting immunocompromised conditions: human immunodeficiency virus (HIV(+)), diabetes mellitus type 2, cancer, tuberculosis and alcoholism. Serum samples from 93 individuals were analyzed by ELISA, as follows: Group 1: 30 immunocompromised individuals with strongyloidiasis; Group 2: 33 immunocompromised individuals without strongyloidiasis and Group 3: 30 healthy individuals. The total saline extract (SE) and detergent fraction (D) showed a sensitivity of 73.33 and 83.33%, and specificity of 82.15 and 86.36%, respectively. The detergent fraction was effective to detect anti-S. stercoralis IgG antibodies in immunocompromised individuals with strongyloidiasis and may be applied as an important tool in the immunodiagnosis of human strongyloidiasis related to immunosuppression.
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Brazil.
authors.forename: Herculano
authors.initials: H
authors.lastname: da Silva
authors.affiliation: Ambulatório DST - "Herbert de Souza", Doenças Sexualmente Transmissíveis, Secretaria Municipal de Saúde, Prefeitura Municipal de Uberlândia, Uberlândia, Brazil.
authors.forename: Célio José Victal
authors.initials: CJ
authors.lastname: de Carvalho
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Brazil.
authors.forename: Marcelo Arantes
authors.initials: MA
authors.lastname: Levenhagen
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Brazil. Electronic address: costacruz@ufu.br.
authors.forename: Julia Maria
authors.initials: JM
authors.lastname: Costa-Cruz
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D003902:Detergents; D004797:Enzyme-Linked Immunosorbent Assay; D006373:Helminthiasis / Q000175:diagnosis* / Q000469:parasitology; D006801:Humans; D016867:Immunocompromised Host; D007074:Immunoglobulin G / Q000097:blood*; D007159:Immunologic Tests; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis* / Q000469:parasitology; D012680:Sensitivity and Specificity; D017171:Strongyloides stercoralis / Q000276:immunology* / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D003902:Detergents; D007074:Immunoglobulin G
keywords: Immunocompromised; Immunodiagnosis; Strongyloides venezuelensis; Strongyloidiasis