title: [Molecular identification of Mentha haplocalyx and Mentha spicata with specific primers multi-PCR system].
abstract: OBJECTIVE
  To screen specific SNPs loci of Mentha haplocalyx and Mentha spicata,and then specific primers were designed to identify the two species and their mixture rapidly.
  
  
  METHODS
  PsbA-trnH sequences of Mentha haplocalyx and Mentha spicata were obtained by PCR product sequencing and downloading from GenBank. SNPs in the psbA-trnH sequences of Mentha haplocalyx and Mentha spicata were found by ClustulW program and Bioedit software. Primers for authentication of the two species were designed according to the SNP loci, and PCR reaction system was optimized to identify the original plants.
  
  
  RESULTS
  Multi-PCR reaction system was constructed. The 181 bp identification band for Mentha haplocalyx or(and) 288 bp identification band for Mentha spicata could be produced by a single PCR reaction,which showed good identification ability to the two species.
  
  
  CONCLUSIONS
  The multi-PCR reaction system can be applied to identify Mentha haplocalyx and Mentha spicata as well as their mixture.
authors.forename: Liang
authors.initials: L
authors.lastname: Cao
authors.forename: Shuang-shuang
authors.initials: SS
authors.lastname: Qin
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Xiao-qi
authors.initials: XQ
authors.lastname: Zhu
mesh_terms: D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D004340:Drug Contamination / Q000517:prevention & control; D027541:Mentha / Q000235:genetics*; D031337:Mentha spicata / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011786:Quality Control; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant