title: Mutation of alkyl hydroperoxide reductase gene ahpC of Xanthomonas oryzae pv. oryzae affects hydrogen peroxide accumulation during the rice-pathogen interaction.
abstract: Hydrogen peroxide (H2O2) is usually generated by normal aerobic respiration of pathogens and by the host defense response during plant-pathogen interactions. In this study, histochemical localization of H2O2 accumulation in rice inoculated with the wild-type strain (PXO99(A)) and the gene deletion mutant (ΔahpC) of alkyl hydroperoxide reductase subunit C (AhpC) of Xanthomonas oryzae pv. oryzae (Xoo), the bacterial blight pathogen of rice, was analyzed. The ΔahpC mutant displayed a significant decrease in endogenous H2O2 accumulation which was induced by the compensatory increase in H2O2 scavenging activity. The change in the bacterial endogenous H2O2 level affected the total amount of H2O2 accumulation during the interaction with rice plants. These results suggested that Xoo contributes to H2O2 accumulation in rice in a compatible interaction, and pathogen-driving H2O2 is in association with cell collapse of rice.
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: lixinpxy@hotmail.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China. Electronic address: 17958087@qq.com.
authors.forename: Jiaju
authors.initials: J
authors.lastname: Qiao
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China. Electronic address: yanglipeng6070@163.com.
authors.forename: Lipeng
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China. Electronic address: lixinlingwjj@163.com.
authors.forename: Xinling
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.
authors.forename: Suyu
authors.initials: S
authors.lastname: Qiao
authors.affiliation: Medical Technology and Engineering College, Henan University of Science and Technology, Luoyang 471003, China. Electronic address: 188050296@qq.com.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Pang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: tfcaas@126.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Tian
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: hmchen@ippcaas.cn.
authors.forename: Huamin
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: cyhe@caas.net.cn.
authors.forename: Chenyang
authors.initials: C
authors.lastname: He
mesh_terms: D017353:Gene Deletion; D054884:Host-Pathogen Interactions; D006861:Hydrogen Peroxide / Q000378:metabolism*; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012275:Oryza / Q000382:microbiology*; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D014974:Xanthomonas / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*
chemical_list: D050505:Mutant Proteins; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins
keywords: Alkyl hydroperoxide reductase; Compatible interaction; Hydrogen peroxide; Xanthomonas oryzae pv. oryzae