title: In vitro evaluation of antiproliferative effect of ethyl gallate against human oral squamous carcinoma cell line KB.
abstract: Although some polyphenols are known to possess anticancer activity against different cancer cell lines through induction of apoptosis, the mode of antiproliferative effect of ethyl gallate against human oral squamous carcinoma cell line KB was not studied until now. Therefore, the antiproliferative effect of ethyl gallate was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in comparison with the reference drug paclitaxel. Generation of reactive oxygen species, mitochondrial membrane potential loss, DNA damage and apoptosis were determined using 2,7-diacetyldichlorofluorescein fluorescence, uptake of rhodamine-123 by mitochondria, comet assay and acridine orange/ethidium bromide dual-dye staining method. Both ethyl gallate and paclitaxel exhibited cytotoxicity in a dose-dependent manner. The 50% inhibitory concentration for ethyl gallate was 30 and 20 μg/mL for paclitaxel. A volume of 50 μg/mL of ethyl gallate was found to be significantly effective (P < 0.05) in controlling the cancer cell proliferation leading to acute apoptosis.
authors.affiliation: a Plant Biotechnology Division, School of Bio Sciences and Technology, VIT University , Vellore 632 014 , Tamil Nadu , India.
authors.forename: Shalini
authors.initials: S
authors.lastname: Mohan
authors.forename: Kalaivani
authors.initials: K
authors.lastname: Thiagarajan
authors.forename: Rajasekaran
authors.initials: R
authors.lastname: Chandrasekaran
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D007624:KB Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology*; D017239:Paclitaxel / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016877:Oxidants; D017382:Reactive Oxygen Species; C048734:ethyl gallate; D005707:Gallic Acid; D017239:Paclitaxel
keywords: DNA damage; ROS; antiproliferation; apoptosis; human oral squamous carcinoma cells; paclitaxel