title: Genetic relatedness versus biological compatibility between Aspergillus fumigatus and related species.
abstract: Aspergillus section Fumigati contains 12 clinically relevant species. Among these Aspergillus species, A. fumigatus is the most frequent agent of invasive aspergillosis, followed by A. lentulus and A. viridinutans. Genealogical concordance and mating experiments were performed to examine the relationship between phylogenetic distance and mating success in these three heterothallic species. Analyses of 19 isolates from section Fumigati revealed the presence of three previously unrecognized species within the broadly circumscribed species A. viridinutans. A single mating type was found in the new species Aspergillus pseudofelis and Aspergillus pseudoviridinutans, but in Aspergillus parafelis, both mating types were present. Reciprocal interspecific pairings of all species in the study showed that the only successful crosses occurred with the MAT1-2 isolates of both A. parafelis and A. pseudofelis. The MAT1-2 isolate of A. parafelis was fertile when paired with the MAT1-1 isolates of A. fumigatus, A. viridinutans, A. felis, A. pseudoviridinutans, and A. wyomingensis but was not fertile with the MAT1-1 isolate of A. lentulus. The MAT1-2 isolates of A. pseudofelis were fertile when paired with the MAT1-1 isolate of A. felis but not with any of the other species. The general infertility in the interspecies crossings suggests that genetically unrelated species are also biologically incompatible, with the MAT1-2 isolates of A. parafelis and A. pseudofelis being the exception. Our findings underscore the importance of genealogical concordance analysis for species circumscription, as well as for accurate species identification, since misidentification of morphologically similar pathogens with differences in innate drug resistance may be of grave consequences for disease management.
authors.affiliation: Molecular Microbiology Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH, Bethesda, Maryland, USA.
authors.forename: Janyce A
authors.initials: JA
authors.lastname: Sugui
authors.affiliation: National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Peoria, Illinois, USA.
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Peterson
authors.affiliation: Molecular Microbiology Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH, Bethesda, Maryland, USA.
authors.forename: Abigail
authors.initials: A
authors.lastname: Figat
authors.affiliation: Electron Microscopy Unit, Research Technologies Branch, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana, USA.
authors.forename: Bryan
authors.initials: B
authors.lastname: Hansen
authors.affiliation: Department of Applied and Industrial Mycology, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Samson
authors.affiliation: Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Emilia
authors.initials: E
authors.lastname: Mellado
authors.affiliation: Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
authors.affiliation: Molecular Microbiology Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH, Bethesda, Maryland, USA jkchung@niaid.nih.gov.
authors.forename: Kyung J
authors.initials: KJ
authors.lastname: Kwon-Chung
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000382:microbiology / Q000473:pathology; D001230:Aspergillus / Q000145:classification / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D003433:Crosses, Genetic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D049770:Genes, Mating Type, Fungal; D006801:Humans; D007915:Lepidoptera; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014774:Virulence
chemical_list: D004269:DNA, Bacterial
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