title: Enabling robust quantitative readout in an equipment-free model of device development.
abstract: A critical constraint in the design of appropriate medical devices for the lowest-resource settings is the lack of access to maintenance or repair on instrumentation. There are numerous point-of-care applications for which quantitative readout would have clinical utility. Thus, a challenge to the device developer is to enable quantitative device readout in an equipment-free model that is appropriate for use in even the lowest-resource settings. Paper microfluidics has great potential for enabling equipment-free devices that are low-cost, operable by minimally-trained users, and provide quantitative readout. The focus of this critical review is to describe the work, starting several decades ago and continuing to the present, to enable assays with quantitative readout in a fully-disposable device.
authors.affiliation: School of Chemical, Biological, and Environmental Engineering, Oregon State University, USA. elain.fu@oregonstate.edu.
authors.forename: Elain
authors.initials: E
authors.lastname: Fu
mesh_terms: D003124:Colorimetry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007118:Immunoassay; D046210:Microfluidic Analytical Techniques; D010209:Paper; D019095:Point-of-Care Systems
references.citation: Clin Infect Dis. 2014 Feb;58(3):407-12
references.pmid: 24218101
references.citation: Sci Transl Med. 2012 Sep 19;4(152):152ra129
references.pmid: 22993296
references.citation: Lab Chip. 2013 Jun 21;13(12):2397-404
references.pmid: 23657627
references.citation: Anal Chem. 2014 Feb 18;86(4):2005-12
references.pmid: 24444190
references.citation: J Chromatogr A. 2010 Jun 11;1217(24):3896-9
references.pmid: 20444459
references.citation: Lab Chip. 2013 Jun 21;13(12):2210-51
references.pmid: 23652632
references.citation: Pediatrics. 2013 Feb;131(2):364-82
references.pmid: 23359574
references.citation: Angew Chem Int Ed Engl. 2012 Dec 14;51(51):12707-10
references.pmid: 23144005
references.citation: Clin Chem. 1990 Sep;36(9):1591-7
references.pmid: 2208698
references.citation: Lancet. 1986 Jan 25;1(8474):184-6
references.pmid: 2868208
references.citation: Anal Chem. 2010 Jan 1;82(1):3-10
references.pmid: 20000334
references.citation: J Clin Pathol. 1979 Dec;32(12):1253-6
references.pmid: 317082
references.citation: Clin Chem. 1985 Jul;31(7):1144-50
references.pmid: 3891138
references.citation: PLoS One. 2013 Sep 30;8(9):e75616
references.pmid: 24098705
references.citation: Diabetes Metab. 2003 Apr;29(2 Pt 2):S47-53
references.pmid: 12746627
references.citation: Anal Bioanal Chem. 2009 Feb;393(4):1281-7
references.pmid: 19104785
references.citation: Clin Chem. 1993 Sep;39(9):1948-52
references.pmid: 8375080
references.citation: Anal Chim Acta. 2009 Feb 16;634(1):89-95
references.pmid: 19154815
references.citation: Clin Chem. 1987 Sep;33(9):1521-5
references.pmid: 3304712
references.citation: Clin Chem. 1993 Apr;39(4):619-24
references.pmid: 8472355
references.citation: Clin Pharmacokinet. 1992 Nov;23(5):365-79
references.pmid: 1478004
references.citation: Nat Rev Microbiol. 2004 Mar;2(3):231-40
references.pmid: 15083158
references.citation: Bioanalysis. 2013 Nov;5(22):2821-36
references.pmid: 24256361
references.citation: J Immunol Methods. 2000 Apr 21;238(1-2):55-8
references.pmid: 10758235
references.citation: Anal Chim Acta. 2013 Oct 24;800:50-5
references.pmid: 24120167
references.citation: Anal Chim Acta. 2010 Aug 3;674(2):227-33
references.pmid: 20678634
references.citation: Circulation. 2003 Jan 28;107(3):499-511
references.pmid: 12551878
references.citation: J Immunol Methods. 2008 Jul 20;336(1):30-6
references.pmid: 18442829
references.citation: Anal Chem. 2013 Nov 5;85(21):10432-9
references.pmid: 24074247
references.citation: Epilepsy Res. 1990 Jul;6(2):146-54
references.pmid: 2117529
references.citation: Circulation. 2004 Dec 21;110(25):e545-9
references.pmid: 15611379
references.citation: Clin Chem Lab Med. 2004;42(11):1228-55
references.pmid: 15576287
references.citation: Diagn Microbiol Infect Dis. 2007 Oct;59(2):131-6
references.pmid: 17662560