title: Comparative analysis of diosgenin in Dioscorea species and related medicinal plants by UPLC-DAD-MS.
abstract: BACKGROUND
  Dioscorea is a genus of flowering plants, and some Dioscorea species are known and used as a source for the steroidal sapogenin diosgenin. To screen potential resource from Dioscorea species and related medicinal plants for diosgenin extraction, a rapid method to compare the contents of diosgenin in various plants is crucial.
  
  
  RESULTS
  An ultra-performance liquid chromatography (UPLC) coupled with diode array detection (DAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for identification and determination of diosgenin in various plants. A comprehensive validation of the developed method was conducted. Twenty-four batches of plant samples from four Dioscorea species, one Smilax species and two Heterosmilax species were analyzed by using the developed method.The present method presented good sensitivity, precision and accuracy. Diosgenin was found in three Dioscorea species and one Heterosmilax species, namely D. zingiberensis, D. septemloba, D. collettii and H. yunnanensis.
  
  
  CONCLUSIONS
  The method is suitable for the screening of diosgenin resources from plants. D. zingiberensis is an important resource for diosgenin harvesting.
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, PR China. yitao@hkbu.edu.hk.
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
authors.forename: Lan-Lan
authors.initials: LL
authors.lastname: Fan
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Chen
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Zhu
authors.forename: Hau-Man
authors.initials: HM
authors.lastname: Suen
authors.forename: Yi-Na
authors.initials: YN
authors.lastname: Tang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Chu
authors.initials: C
authors.lastname: Chu
authors.forename: Zhong-Zhen
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: Hu-Biao
authors.initials: HB
authors.lastname: Chen
mesh_terms: D002623:Chemistry Techniques, Analytical; D002681:China; D002851:Chromatography, High Pressure Liquid; D027742:Dioscorea; D004144:Diosgenin / Q000032:analysis*; D005240:Feasibility Studies; D029001:Herbal Medicine / Q000379:methods; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D032281:Smilax; D021241:Spectrometry, Mass, Electrospray Ionization; D014466:Ultraviolet Rays
chemical_list: D010936:Plant Extracts; D004144:Diosgenin
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