title: Concise review: understanding clonal dynamics in homeostasis and injury through multicolor lineage tracing.
abstract: Lineage tracing is an essential tool to study stem cell fate. Although traditional lineage tracing techniques have considerably advanced our understanding of stem cell behavior, they pose significant limitations for identification and longitudinal tracking of the progeny of individual stem cells, to compare their behaviors. This is of importance given the well-established heterogeneity among stem cells both in terms of potentialities and proliferative capacities. The recent development of multicolor genetic reporters addressable to specific cell populations largely overcomes these issues. These new "rainbow" technologies provide increased resolution in clonal identification and offer the possibility to study the relative distribution, contacts, tiled arrangement, and competitive interactions among cells or groups of cells of the same type.
authors.affiliation: Experimental Dermatology Group, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
authors.forename: Edwige
authors.initials: E
authors.lastname: Roy
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Neufeld
authors.forename: Jean
authors.initials: J
authors.lastname: Livet
authors.forename: Kiarash
authors.initials: K
authors.lastname: Khosrotehrani
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D017930:Genes, Reporter / Q000235:genetics / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology*; D006801:Humans; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
keywords: Cre-loxP system; Fluorescent protein reporter genes; Lineage tracing; Progenitor cells; Technology