title: Genome analysis of NDM-1 producing Morganella morganii clinical isolate.
abstract: OBJECTIVE
  To analyze the resistome and virulence genes of Morganella morganii F675, a multidrug-resistant clinical isolate using whole genome sequencing (WGS).
  
  
  METHODS
  M. morganii F675 was isolated from a patient from Jerusalem, Israel. WGS was performed using both 454 and SOLiD sequencing technologies. Analyses of the bacterial resistome and other virulence genes were performed in addition to comparison with other available M. morganii genomes.
  
  
  RESULTS
  The assembled sequence had a genome size of 4,127,528 bp with G+C content of 51%. The resistome consisted of 13 antibiotic resistance genes including blaNDM-1 located in a plasmid likely acquired from Acinetobacter spp. Moreover, we characterized for the first time the whole lipid A biosynthesis pathway in this species along with the O-antigen gene cluster, the urease gene cluster and several other virulence genes.
  
  
  CONCLUSIONS
  The WGS analysis of this pathogen further provides insight into its pathogenicity and resistance to antibiotics.
authors.affiliation: Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE) CNRS-IRD UMR 6236, Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.
authors.forename: Abiola Olumuyiwa
authors.initials: AO
authors.lastname: Olaitan
authors.forename: Seydina M
authors.initials: SM
authors.lastname: Diene
authors.forename: Sushim Kumar
authors.initials: SK
authors.lastname: Gupta
authors.forename: Amos
authors.initials: A
authors.lastname: Adler
authors.forename: Marc Victor
authors.initials: MV
authors.lastname: Assous
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Rolain
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D020613:Morganella morganii / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity / Q000648:ultrastructure; D005810:Multigene Family; D019081:O Antigens / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014510:Urease / Q000235:genetics; D014774:Virulence / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D019081:O Antigens; D014510:Urease; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1
keywords: NDM-1; O-antigen; intrinsic colistin resistance; multidrug-resistant bacteria; resistome; virulence genes