title: Beneficial effects of heat-treated Enterococcus faecalis FK-23 on high-fat diet-induced hepatic steatosis in mice.
abstract: A high-fat diet (HFD) is one of the causes of hepatic steatosis. We previously demonstrated that Enterococcus faecalis FK-23 (FK-23), a type of lactic acid bacteria, exhibits an anti-obesity effect in mice fed a HFD. In the present study, we examined the effects of FK-23 on HFD-induced hepatic steatosis. Male C57BL/6 mice were divided into four groups and given one of four treatments: standard diet (SD); standard diet supplemented with FK-23 (SD+FK); HFD; or HFD supplemented with FK-23 (HFD+FK). For the administration of FK-23, the drinking water was supplemented with FK-23 at a concentration of 2% (w/w). After 11 weeks, histological findings revealed hepatic steatosis in the liver of HFD-fed mice; however, this effect was attenuated by the administration of FK-23. The expression levels of genes involved in fatty acid oxidation in the liver tissue were significantly reduced in the HFD group compared with the SD group, but FK-23 supplementation tended to up-regulate the expression levels of these genes. Our findings show that the inhibitory effect of FK-23 against hepatic steatosis in HFD-fed mice can be explained by the prevention of fat accumulation in the liver through the modulation of the activities of genes involved in hepatic fatty acid oxidation.
authors.affiliation: Central Research Laboratories, Nichinichi Pharmaceutical Corporation Limited,239-1 Tominaga, Iga,518-1417Mie,Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kondoh
authors.affiliation: Central Research Laboratories, Nichinichi Pharmaceutical Corporation Limited,239-1 Tominaga, Iga,518-1417Mie,Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimada
authors.affiliation: Central Research Laboratories, Nichinichi Pharmaceutical Corporation Limited,239-1 Tominaga, Iga,518-1417Mie,Japan.
authors.forename: Kazutake
authors.initials: K
authors.lastname: Fukada
authors.affiliation: Department of Molecular Gastroenterology and Hepatology,Kyoto Prefectural University of Medicine,Kamigyo-ku, Kyoto602-8566,Japan.
authors.forename: Mayuko
authors.initials: M
authors.lastname: Morita
authors.affiliation: Department of Molecular Gastroenterology and Hepatology,Kyoto Prefectural University of Medicine,Kamigyo-ku, Kyoto602-8566,Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Katada
authors.affiliation: Department of Molecular Gastroenterology and Hepatology,Kyoto Prefectural University of Medicine,Kamigyo-ku, Kyoto602-8566,Japan.
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Higashimura
authors.affiliation: Department of Molecular Gastroenterology and Hepatology,Kyoto Prefectural University of Medicine,Kamigyo-ku, Kyoto602-8566,Japan.
authors.forename: Katsura
authors.initials: K
authors.lastname: Mizushima
authors.affiliation: Central Research Laboratories, Nichinichi Pharmaceutical Corporation Limited,239-1 Tominaga, Iga,518-1417Mie,Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Okamori
authors.affiliation: Department of Molecular Gastroenterology and Hepatology,Kyoto Prefectural University of Medicine,Kamigyo-ku, Kyoto602-8566,Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Naito
authors.affiliation: Department of Gastrointestinal Immunology,Kyoto Prefectural University of Medicine,Kamigyo-ku, Kyoto602-8566,Japan.
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000627:therapeutic use; D059305:Diet, High-Fat / Q000009:adverse effects; D019587:Dietary Supplements; D013293:Enterococcus faecalis / Q000737:chemistry*; D005227:Fatty Acids / Q000378:metabolism; D005612:Freeze Drying; D005786:Gene Expression Regulation; D006358:Hot Temperature; D006943:Hyperglycemia / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D008066:Lipolysis; D008082:Lipotropic Agents / Q000627:therapeutic use*; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009929:Organ Size
chemical_list: D019440:Anti-Obesity Agents; D005227:Fatty Acids; D007004:Hypoglycemic Agents; D008082:Lipotropic Agents