title: ABCG2 is a selectable marker for enhanced multilineage differentiation potential in periodontal ligament stem cells.
abstract: Periodontal ligament stem cells (PDLSCs) provide an important source for tissue regeneration and may become especially useful in the formation of osteogenic seeds. PDLSCs can be cultured, expanded, and differentiated in vitro; thus, they may be applied in the long-term treatment of the defects in the dental regions. Here we studied numerous potential markers allowing the selection of human PDLSCs with a maximum differentiation potential. We followed the expression of the ATP-binding cassette subfamily G member 2 (ABCG2) membrane transporter protein and isolated ABCG2-expressing cells by using a monoclonal antibody, recognizing the transporter at the cell surface in intact cells. The expression of the ABCG2 protein, corresponding to the so-called side-population phenotype in various tissue-derived stem cells, was found to be a useful marker for the selection of PDLSCs with enhanced osteogenic, chondrogenic, and adipogenic differentiation. These findings may have important applications in achieving efficient dental tissue regeneration by using stem cells from extracted teeth.
authors.affiliation: 1 Research Centre for Natural Sciences, Hungarian Academy of Sciences , Budapest, Hungary .
authors.forename: Áron
authors.initials: Á
authors.lastname: Szepesi
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Matula
authors.forename: Anna
authors.initials: A
authors.lastname: Szigeti
authors.forename: György
authors.initials: G
authors.lastname: Várady
authors.forename: Gyula
authors.initials: G
authors.lastname: Szabó
authors.forename: Ferenc
authors.initials: F
authors.lastname: Uher
authors.forename: Balázs
authors.initials: B
authors.lastname: Sarkadi
authors.forename: Katalin
authors.initials: K
authors.lastname: Német
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000943:Antigens, Differentiation / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D000943:Antigens, Differentiation; D009363:Neoplasm Proteins
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