title: The toxicity of a lipid transfer protein (Cc-LTP1) from Coffea canephora Seeds on the larval development of Callosobruchus maculatus (Coleoptera: Bruchidae).
abstract: In this work, we analyzed the effects of coffee seed proteins, especially Cc-LTP1 on the larval development of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), a bruchid pest of beans and the most important insect pest of Vigna unguiculata (L.) Walp. Artificial seed assay, which incorporated the F/0-90 fraction from Coffea canephora seeds, resulted in the reduction of oviposition and caused an inhibition of C. maculatus larval development in a dose-dependent manner. The F/0-90 fraction used at a 4 % concentration resulted in the survival of no larvae. The purified Cc-LTP1, at a concentration of 0.5 %, also demonstrated effective inhibition of larval development, reducing both females oviposition and the weight and number of larvae. Cc-LTP1 was also able to inhibit the C. maculatus gut α-amylase activity, and immunolabeling by an anti-LTP serum was observed in the midgut tissues of the C. maculatus larvae. Cc-LTP1 has shown binding affinity towards microvillar cells, endoplasmic reticulum and mitochondria, as demonstrated by micrographic images taken by a transmission electron microscope. The results from this study indicate that Cc-LTP1 has insecticidal actions toward C. maculatus and exerts anti-nutritional effects with direct actions on intestinal tissues.
authors.affiliation: Laboratório de Microbiologia Ambiental e Biotecnologia, Complexo Biopráticas, Universidade Vila Velha, Vila Velha, ES, Brazil.
authors.forename: Umberto
authors.initials: U
authors.lastname: Zottich
authors.forename: Maura
authors.initials: M
authors.lastname: Da Cunha
authors.forename: Germana B
authors.initials: GB
authors.lastname: Dias
authors.forename: Guilherme R
authors.initials: GR
authors.lastname: Rabelo
authors.forename: Antonia Elenir A
authors.initials: AE
authors.lastname: Oliveira
authors.forename: André O
authors.initials: AO
authors.lastname: Carvalho
authors.forename: Kátia Valevski S
authors.initials: KV
authors.lastname: Fernandes
authors.forename: Viviane V
authors.initials: VV
authors.lastname: do Nascimento
authors.forename: Valdirene M
authors.initials: VM
authors.lastname: Gomes
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000633:toxicity*; D040503:Coffea / Q000737:chemistry*; D001517:Coleoptera / Q000187:drug effects* / Q000254:growth & development; D005260:Female; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D012639:Seeds / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; C030363:lipid transfer protein
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