title: Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology.
abstract: We demonstrated the use of a new organometallic nanoprobe for competitive surface-enhanced Raman scattering (SERS) immunoassay devoted to the detection of polycyclic aromatic hydrocarbons (PAH) such as benzo[a]pyrene (BaP) in seawater. The nanoprobes are gold nanoparticles (GNPs) labeled by a Raman reporter, the 5,5'-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB) and functionalized with monoclonal antibodies anti-BaP. The antibodies are bound with a high specificity to the analyte while the GNPs enhanced the Raman scattering of the DSNB. This type of immunoassay involved the grafting of BaP onto a sensing surface. Thus, NH2-terminated self-assembled monolayer is formed on the surface of gold substrate using cysteamine. Amines finally reacted with 6-formylbenzo[a]pyrene. So, this SERS detection involves four steps: (i) the nanoprobes are incubated with the sample; (ii) a drop of the mixture is then put onto the substrate; (iii) the surface is rinsed; and (iv) the surface is analyzed by Raman spectroscopy. To synthesize the nanoprobes, firstly, we prepared GNPs according to Frens' method. Then, GNPs were spontaneously labeled by the DSNB Raman reporter, thanks to a strong gold-sulfur interaction. Thereafter, BaP antibodies were cross-linked to the DSNB labeled GNPs by reaction of proteins primary amino groups with N-hydroxyl succinimide (NHS). Before use in SERS detection, their activity was controlled by surface plasmon resonance technique. The present method allows us to detect BaP at trace concentration (2 nmol/L). The results demonstrate that the proposed method has a great potential for application in the monitoring of seawater.
authors.affiliation: IFREMER - Laboratoire Détection, Capteurs et Mesures, IFREMER, CS10070, 29280, Plouzané, France.|EA 6295 Nanomédicaments et Nanosondes, UFR des Sciences Pharmaceutiques, Université François-Rabelais de Tours, 31 avenue Monge, 37200, Tours, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Dribek
authors.affiliation: IFREMER - Laboratoire Détection, Capteurs et Mesures, IFREMER, CS10070, 29280, Plouzané, France. emmanuel.rinnert@ifremer.fr.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Rinnert
authors.affiliation: IFREMER - Laboratoire Détection, Capteurs et Mesures, IFREMER, CS10070, 29280, Plouzané, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Colas
authors.affiliation: IFREMER - Laboratoire Détection, Capteurs et Mesures, IFREMER, CS10070, 29280, Plouzané, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Crassous
authors.affiliation: Laboratoire CSPBAT (FRE 3043), UFR SMBH, Université Paris 13, 74 rue Marcel Cachin, 93017, Bobigny, France.
authors.forename: Néné
authors.initials: N
authors.lastname: Thioune
authors.affiliation: Laboratoire CSPBAT (FRE 3043), UFR SMBH, Université Paris 13, 74 rue Marcel Cachin, 93017, Bobigny, France.|Horiba Scientific, 231 rue de, Lille, 59650, Villeneuve d'Ascq, France.
authors.forename: Catalina
authors.initials: C
authors.lastname: David
authors.affiliation: Laboratoire CSPBAT (FRE 3043), UFR SMBH, Université Paris 13, 74 rue Marcel Cachin, 93017, Bobigny, France.
authors.forename: Marc
authors.initials: M
authors.lastname: de la Chapelle
authors.affiliation: IFREMER - Laboratoire Détection, Capteurs et Mesures, IFREMER, CS10070, 29280, Plouzané, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Compère
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D001564:Benzo(a)pyrene / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay; D053768:Metal Nanoparticles / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D012623:Seawater / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D009942:Organometallic Compounds; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D006046:Gold
keywords: Benzo[a]pyrene antibody; Biosensor; Organometallic nanoprobe; Polycyclic aromatic hydrocarbons; Surface-enhanced Raman scattering
references.citation: Mar Pollut Bull. 2004 Aug;49(3):229-34
references.pmid: 15245987
references.citation: Science. 1997 Feb 21;275(5303):1102-6
references.pmid: 9027306
references.citation: Analyst. 2012 Sep 7;137(17):4010-5
references.pmid: 22783547
references.citation: J Biomed Opt. 2005 May-Jun;10(3):031112
references.pmid: 16229637
references.citation: Appl Spectrosc. 2010 Oct;64(10):1086-93
references.pmid: 20925977
references.citation: Fresenius J Anal Chem. 2001 Nov;371(5):578-85
references.pmid: 11767882
references.citation: J Colloid Interface Sci. 2014 Mar 15;418:87-94
references.pmid: 24461822
references.citation: J Org Chem. 2007 Jun 22;72(13):4856-63
references.pmid: 17530898
references.citation: Mar Pollut Bull. 2012 Mar;64(3):614-26
references.pmid: 22248648
references.citation: Talanta. 2009 Jul 15;79(2):199-204
references.pmid: 19559865
references.citation: Anal Chem. 2007 Jun 1;79(11):4215-21
references.pmid: 17458937
references.citation: Anal Chem. 2003 Nov 1;75(21):5936-43
references.pmid: 14588035