title: Estimation of optoacoustic contrast agent concentration with self-calibration blind logarithmic unmixing.
abstract: Chromophore quantification in optoacoustic tomography is challenging due to signal contributions from strongly absorbing background tissue chromophores and the depth-dependent light attenuation. Herein we present a procedure capable of correcting for wavelength-dependent light fluence variations using a logarithmic representation of the images taken at different wavelengths assisted with a blind unmixing approach. It is shown that the serial expansion of the logarithm of an optoacoustic image contains a term representing the ratio between absorption of the probe of interest and other background components. Under assumptions of tissue-like background absorption variations, this term can be readily isolated with an unmixing algorithm, attaining quantitative maps of photo-absorbing agent distribution.
authors.affiliation: Institute for Biological and Medical Imaging, (IBMI), Helmholtz Center Munich, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany.
authors.forename: X Luís
authors.initials: XL
authors.lastname: Deán-Ben
authors.forename: Andreas
authors.initials: A
authors.lastname: Buehler
authors.forename: Daniel
authors.initials: D
authors.lastname: Razansky
authors.forename: Vasilis
authors.initials: V
authors.lastname: Ntziachristos
mesh_terms: D000465:Algorithms; D002138:Calibration; D003287:Contrast Media / Q000592:standards*; D008027:Light; D041622:Tomography, Optical / Q000379:methods* / Q000592:standards; D014463:Ultrasonography / Q000379:methods* / Q000592:standards
chemical_list: D003287:Contrast Media