title: Monolithic silica columns functionalized with substituted polyproline-derived chiral selectors as chiral stationary phases for high-performance liquid chromatography.
abstract: In this study, two polyproline-derived chiral selectors are bonded to monolithic silica gel columns. In spite of high chiral selector coverage, the derivatization was found to have only a slight effect on the hydrodynamics of the mobile phase through the column. The enantioseparation ability of the resulting chiral monolithic columns was evaluated with a series of structurally diverse racemic test compounds. When compared to analogous bead-based chiral stationary phases, higher enantioseparation and broader application domain were observed for monolithic columns. Moreover, the increase in flow rate produces a minor reduction of resolution, which permits to shorten analysis time. Additionally, increased loadability defines chiral polyproline derived monoliths as adequate for preparative chromatography.
authors.affiliation: Food and Nutrition Torribera Campus, University of Barcelona, Santa Coloma de Gramenet, Barcelona, Spain; Laboratori de Química Farmacèutica, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Sancho
authors.forename: Arnau
authors.initials: A
authors.lastname: Novell
authors.forename: Frantisek
authors.initials: F
authors.lastname: Svec
authors.forename: Cristina
authors.initials: C
authors.lastname: Minguillón
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D010455:Peptides / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D010455:Peptides; C011083:polyproline; D012822:Silicon Dioxide
keywords: Chiral selectors; Chiral stationary phases; Enantioseparation; Poly-l-proline oligomers; Silica monoliths