title: Rapid flow through immunoassay for CRP determination based on polyethylene filters modified with ω-aminocellulose carbamate.
abstract: A novel method for antibody immobilization is discussed and applied in a column based flow through immunoassay system (ABICAP). It is shown that porous polyethylene material can be modified with different ω-aminocellulose carbamates yielding an amino group containing biocompatible support for antibody immobilization. Anti-h CRP antibodies can be bound covalently to the surface via various homobifunctional cross-linkers. The antibody modified filters are validated for the CRP determination in a sandwich ABICAP system. In a 10 min test procedure based on colloidal dye particles, a limit of detection of 5 ng CRP mL(-1) and coefficients of variation of <9.1% are obtained.
authors.affiliation: Center of Excellence for Polysaccharide Research, Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University of Jena, Humboldtstraße 10, D-07743, Jena, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Elschner
authors.forename: Friedrich
authors.initials: F
authors.lastname: Scholz
authors.forename: Peter
authors.initials: P
authors.lastname: Miethe
authors.forename: Thomas
authors.initials: T
authors.lastname: Heinze
mesh_terms: D000906:Antibodies / Q000378:metabolism; D002097:C-Reactive Protein / Q000378:metabolism*; D002138:Calibration; D002219:Carbamates / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D007118:Immunoassay / Q000379:methods*; D020959:Polyethylene / Q000737:chemistry*
chemical_list: D000906:Antibodies; D002219:Carbamates; D055767:Immobilized Proteins; D020959:Polyethylene; D002482:Cellulose; D002097:C-Reactive Protein
keywords: ABICAP; C-reactive protein; biosensors; film formation; ω-aminocellulose carbamate