title: Analysis of oxidative stress enzymes and structural and functional proteins on human aortic tissue from different aortopathies.
abstract: The role of oxidative stress in different aortopathies is evaluated. Thirty-two tissue samples from 18 men and 14 women were divided into: 4 control (C) subjects, 11 patients with systemic arterial hypertension (SAH), 4 with variants of Marfan's syndrome (MV), 9 with Marfan's syndrome (M), 2 with Turner's syndrome, and 2 with Takayasu's arteritis (TA). Aorta fragments were homogenized. Lipoperoxidation (LPO), copper-zinc and manganese superoxide dismutase (Mn and Cu-Zn-SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), endothelial nitric oxide synthase (eNOS), nitrates and nitrites (NO3(-)/NO2(-)), and type IV collagen, and laminin were evaluated. There was an increase in Mn- and Cu-Zn-SOD activity in SAH, MV, M, and Turner's syndrome. There was also an increase in CAT activity in M and Turner' syndrome. GPx and GST activity decreased and LPO increased in all groups. eNOS was decreased in SAH, MV, and M and NO3 (-)/NO2 (-) were increased in SAH and TA. Type IV collagen was decreased in Turner's syndrome and TA. Laminin γ-1 was decreased in MV and increased in M. In conclusion, similarities and differences in oxidative stress in the different aortopathies studied including pathologies with aneurysms were found with alterations in SOD, CAT, GPx, GST, and eNOS activity that modify subendothelial basement membrane proteins.
authors.affiliation: Immunology Department, National Institute of Cardiology "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Soto
authors.affiliation: Pathology Department, National Institute of Cardiology "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Soria-Castro
authors.affiliation: Physiology Department, National Institute of Cardiology "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico.
authors.forename: Verónica Guarner
authors.initials: VG
authors.lastname: Lans
authors.affiliation: Cardiovascular Surgery Department, National Institute of Cardiology "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico.
authors.forename: Eleazar Muruato
authors.initials: EM
authors.lastname: Ontiveros
authors.affiliation: Cardiovascular Surgery Department, National Institute of Cardiology "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico.
authors.forename: Benjamín Iván Hernández
authors.initials: BI
authors.lastname: Mejía
authors.affiliation: Cardiovascular Surgery Department, National Institute of Cardiology "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico.
authors.forename: Humberto Jorge Martínez
authors.initials: HJ
authors.lastname: Hernandez
authors.affiliation: Cardiovascular Surgery Department, National Institute of Cardiology "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico.
authors.forename: Rodolfo Barragán
authors.initials: RB
authors.lastname: García
authors.affiliation: Cardiovascular Surgery Department, National Institute of Cardiology "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico.
authors.forename: Valentín
authors.initials: V
authors.lastname: Herrera
authors.affiliation: Pathology Department, National Institute of Cardiology "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico.
authors.forename: Israel
authors.initials: I
authors.lastname: Pérez-Torres
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001013:Aorta, Thoracic / Q000000981:diagnostic imaging / Q000201:enzymology*; D001018:Aortic Diseases / Q000000981:diagnostic imaging / Q000201:enzymology* / Q000473:pathology; D002374:Catalase / Q000032:analysis; D024141:Collagen Type IV / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis; D005982:Glutathione Transferase / Q000032:analysis; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000032:analysis; D052250:Nitric Oxide Synthase Type III / Q000032:analysis; D010088:Oxidoreductases / Q000032:analysis*; D013482:Superoxide Dismutase / Q000032:analysis; D014057:Tomography, X-Ray Computed; D055815:Young Adult
chemical_list: D024141:Collagen Type IV; D010088:Oxidoreductases; D002374:Catalase; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
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