title: Effects of bone morphogenetic protein 4 (BMP4) supplementation during culture of the sheep ovarian cortex.
abstract: The aim of the present study was to establish the presence of BMP type I and II receptor presence in preantral follicles and to investigate the effect of BMP4 supplementation on preantral follicle activation and development during organotypic culture of prepubertal ovine ovarian cortex pieces. Ovine ovarian fragments were cultured with varying concentrations (0, 25, 50 or 100 ng/ml) of BMP4 in the presence or absence of FSH in the culture media to determine the optimal minimum dose for preantral follicle activation and development. Follicular morphometry, immunohistochemistry for BMPR-IA, BMPR-IB, BMPR-II, proliferating cell nuclear antigen and TUNEL progesterone and 17β-oestradiol production were assessed. Follicle and oocyte diameter were positively influenced by the addition of BMP4 to culture. However, there was no effect of BMP4 on primordial follicle activation. Treatment with BMP4 reduced progesterone synthesis but had no effect on oestradiol. The percentage of primordial follicles stained with TUNEL was significantly greater in the culture without BMP4 compared to other culture systems after 9 days of culture. There was an increase in PCNA immunoreactivity in all follicles regardless of treatment. Treatment with BMP4 increases the size of oocytes and follicles but has minimal effects on follicular dynamics during in vitro culture of ovarian cortical tissue of sheep.
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bertoldo
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; MNHN, Laboratoire de la Réserve de la Haute Touche, 36290 Obterre, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Duffard
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; MNHN, Laboratoire de la Réserve de la Haute Touche, 36290 Obterre, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Bernard
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; Service de Médecine et Biologie de la Reproduction, CHRU de Tours, Tours, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37041 Tours, France.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Frapsauce
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Calais
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France.
authors.forename: Charlène
authors.initials: C
authors.lastname: Rico
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37041 Tours, France; IFCE, F-37380 Nouzilly, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Mermillod
authors.affiliation: INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; MNHN, Laboratoire de la Réserve de la Haute Touche, 36290 Obterre, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37041 Tours, France; IFCE, F-37380 Nouzilly, France. Electronic address: yann.locatelli@tours.inra.fr.
authors.forename: Yann
authors.initials: Y
authors.lastname: Locatelli
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000494:pharmacology*; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics / Q000378:metabolism; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000235:genetics / Q000378:metabolism; D005260:Female; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D012756:Sheep / Q000502:physiology*; D046509:Tissue Culture Techniques / Q000662:veterinary*
chemical_list: D055415:Bone Morphogenetic Protein 4; D018809:Proliferating Cell Nuclear Antigen; D052005:Bone Morphogenetic Protein Receptors, Type I; D052006:Bone Morphogenetic Protein Receptors, Type II
keywords: BMP4 receptor; Bone morphogenetic proteins; Follicle activation; In vitro folliculogenesis; Steroids