title: Laccase biosensor based on phytic acid modification of nanostructured SiO₂ surface for sensitive detection of dopamine.
abstract: In this work, three kinds of nanostructured silica-phytic acid (SiO2-PA) materials with diverse morphologies including spherical SiO2-PA (s-SiO2-PA), rod-like (r-SiO2-PA), and helical SiO2-PA (h-SiO2-PA) were prepared with the help of electrostatic interaction. The SiO2-PA nanomaterials with different morphologies were characterized by using transmission electron microscopy (TEM), Fourier transform infrared (FTIR), electrochemical impedance spectroscopy (EIS), and circular dichroism spectrum (CD). Diverse morphologies of SiO2-PA were used as electrode decorated materials to achieve a high efficiency for electrochemical dopamine (DA) detection. The laccase biosensors were fabricated by immobilizing different morphologies of SiO2-PA nanomaterials and laccase onto the glassy carbon electrode (GCE) surface, successively. Then the electrochemical responses of the different morphologies of nanostructured SiO2-PA nanomaterials to laccase were discussed. Results indicated that compared to laccase/s-SiO2-PA and laccase/r-SiO2-PA, the laccase/h-SiO2-PA-modified electrode showed the best electrochemical performances.
authors.affiliation: National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing 210023, People's Republic of China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhao
authors.forename: Kuai
authors.initials: K
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wei
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Ma
authors.forename: Lingling
authors.initials: L
authors.lastname: Liu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Huang
mesh_terms: D015374:Biosensing Techniques; D004298:Dopamine / Q000032:analysis*; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D010833:Phytic Acid / Q000737:chemistry* / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D012822:Silicon Dioxide; D010833:Phytic Acid; D042845:Laccase; D004298:Dopamine