title: Production of conjugated linoleic and conjugated α-linolenic acid in a reconstituted skim milk-based medium by bifidobacterial strains isolated from human breast milk.
abstract: Eight bifidobacterial strains isolated from human breast milk have been tested for their abilities to convert linoleic acid (LA) and α-linolenic acid (LNA) to conjugated linoleic acid (CLA) and conjugated α-linolenic acid (CLNA), respectively. These bioactive lipids display important properties that may contribute to the maintenance and improvement human health. Three selected Bifidobacterium breve strains produced CLA from LA and CLNA from LNA in MRS (160-170 and 210-230 μg mL(-1), resp.) and, also, in reconstituted skim milk (75-95 and 210-244 μg mL(-1), resp.). These bifidobacterial strains were also able to simultaneously produce both CLA (90-105 μg mL(-1)) and CLNA (290-320 μg mL(-1)) in reconstituted skim milk. Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids. To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.
authors.affiliation: Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM+CSIC, C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain ; Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: María Antonia
authors.initials: MA
authors.lastname: Villar-Tajadura
authors.affiliation: Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM+CSIC, C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain.
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: Rodríguez-Alcalá
authors.affiliation: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Martín
authors.affiliation: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Aránzazu
authors.initials: A
authors.lastname: Gómez de Segura
authors.affiliation: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Juan Miguel
authors.initials: JM
authors.lastname: Rodríguez
authors.affiliation: Departamento de Biotecnología y Microbiología de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM+CSIC, C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain.
authors.forename: Teresa
authors.identifier: 0000-0002-8846-6846
authors.initials: T
authors.lastname: Requena
authors.affiliation: Departamento de Bioactividad y Análisis de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) CEI UAM+CSIC, C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Fontecha
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D002849:Chromatography, Gas; D003470:Culture Media / Q000737:chemistry*; D005260:Female; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*; D008892:Milk / Q000737:chemistry*; D008895:Milk, Human / Q000382:microbiology*; D013997:Time Factors; D017962:alpha-Linolenic Acid / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D044243:Linoleic Acids, Conjugated; D017962:alpha-Linolenic Acid
references.citation: Pediatrics. 2006 Apr;117(4):e760-8
references.pmid: 16585287
references.citation: J Appl Microbiol. 2003;94(1):138-45
references.pmid: 12492934
references.citation: Lipids. 2012 Mar;47(3):313-27
references.pmid: 22160449
references.citation: Int J Food Microbiol. 2012 Jan 16;152(3):189-205
references.pmid: 21742394
references.citation: Talanta. 2014 Oct;128:518-23
references.pmid: 25059195
references.citation: Nutr Cancer. 2006;56(1):95-102
references.pmid: 17176223
references.citation: Appl Microbiol Biotechnol. 2010 Aug;87(6):2257-66
references.pmid: 20556602
references.citation: Lancet. 1994 Oct 15;344(8929):1046-9
references.pmid: 7934445
references.citation: Appl Environ Microbiol. 2009 Feb;75(4):965-9
references.pmid: 19088308
references.citation: Prog Lipid Res. 2001 Jul;40(4):283-98
references.pmid: 11412893
references.citation: Lipids. 2009 Mar;44(3):249-56
references.pmid: 19048324
references.citation: Bacteriol Rev. 1954 Jun;18(2):147-63
references.pmid: 13159785
references.citation: Int J Food Microbiol. 2011 Sep 1;149(1):88-105
references.pmid: 21763022
references.citation: J Dairy Sci. 2005 Sep;88(9):3231-9
references.pmid: 16107413
references.citation: Biochim Biophys Acta. 1997 Mar 10;1345(1):5-10
references.pmid: 9084496
references.citation: Gut. 2002 Jul;51(1):51-5
references.pmid: 12077091
references.citation: Lett Appl Microbiol. 2007 May;44(5):467-74
references.pmid: 17451511
references.citation: Lipids. 2001 Nov;36(11):1283-7
references.pmid: 11795862
references.citation: J Appl Microbiol. 2011 Sep;111(3):593-606
references.pmid: 21699633
references.citation: J Appl Microbiol. 1998 Jul;85(1):95-102
references.pmid: 9721660
references.citation: J Hum Lact. 2012 Feb;28(1):36-44
references.pmid: 22267318
references.citation: J Pediatr Gastroenterol Nutr. 2004 Aug;39(2):147-52
references.pmid: 15269618
references.citation: J Nutr Biochem. 2001 Nov;12(11):622-630
references.pmid: 12031255
references.citation: Int Arch Allergy Immunol. 2006;140(2):157-63
references.pmid: 16601353
references.citation: Lipids. 2011 Feb;46(2):105-19
references.pmid: 21161605
references.citation: J Agric Food Chem. 2008 Nov 26;56(22):10908-12
references.pmid: 18973338
references.citation: J Pediatr. 2003 Dec;143(6):754-8
references.pmid: 14657823
references.citation: Int J Food Microbiol. 1999 Sep 15;50(1-2):45-57
references.pmid: 10488843
references.citation: Appl Environ Microbiol. 2011 Oct;77(19):6788-93
references.pmid: 21821739
references.citation: J Biol Chem. 1966 Mar 25;241(6):1350-4
references.pmid: 5936712
references.citation: J Nutr. 2000 Sep;130(9):2285-91
references.pmid: 10958825
references.citation: J Lipid Res. 2009 Dec;50(12):2412-20
references.pmid: 19542528