title: Amides from Piper as a Diuretic: Behind the Ethnopharmacological Uses of Piper glabratum Kunth.
abstract: Several species of the genus Piper are known in Brazilian folk medicine as having diuretic activity. So, we propose to investigate the acute diuretic activity and the possible toxic effects of Piper glabratum Kunth, popularly known as false Jaborandi. Additionally, we propose to check whether there is any correlation between the biological activities of the crude extract (MEPG) and its 2-methoxy-4,5-methylenedioxy-trans-cinnamoyl-pyrrolidine (MMCP) in Wistar rats. The MEPG was fractioned by chromatography column and the MMCP was identified by analyses of (1)H and (13)C RMN spectral data and correlations. Both MEPG and MMCP were assayed for diuretic activity. The preparations obtained were orally administered in a single dose to rats. The urine excretion, pH, density, conductivity, and content of Na(+), K(+), Cl(-), and HCO3 (-) were measured in the urine of saline-loaded animals. Additionally, acute toxicity of the extract was also evaluated. MMCP at doses of 30 mg/kg was able to increase the urine volume, pH, and HCO3 (-) excretion. Moreover, high dosage of MEPG showed important liver toxicity and elevated mortality when injected intraperitoneally. The results indicate that the MMCP shows important diuretic properties when administered in Wistar rats. Additionally, MEPG can induce important acute toxicity if given in high doses.
authors.affiliation: Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil.
authors.forename: Thiago Bruno Lima
authors.initials: TB
authors.lastname: Prando
authors.affiliation: Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil.
authors.forename: Tatiane da Fonseca
authors.initials: Tda F
authors.lastname: Baciquete
authors.affiliation: Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil.
authors.forename: Jennifer Alexandra Castanho
authors.initials: JA
authors.lastname: Vieira
authors.affiliation: Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil.
authors.forename: Jaqueline
authors.initials: J
authors.lastname: Bressan
authors.affiliation: Laboratório de Farmacologia Cardiovascular, Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Rodovia Dourados, Itahum, km 12, P.O. Box 533, 79.804-970 Dourados, MS, Brazil.
authors.forename: Francielly Mourão
authors.initials: FM
authors.lastname: Gasparotto
authors.affiliation: Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil.
authors.forename: Douglas Rossi
authors.initials: DR
authors.lastname: Jesus
authors.affiliation: Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil.
authors.forename: Euclides Lara
authors.initials: EL
authors.lastname: Cardozo Junior
authors.affiliation: Instituto de Ciências Biológicas, Médicas e da Saúde, Universidade Paranaense, P.O. Box 224, 87502-210 Umuarama, PR, Brazil.
authors.forename: Emerson Luiz Botelho
authors.initials: EL
authors.lastname: Lourenço
authors.affiliation: Laboratório de Farmacologia Cardiovascular, Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Rodovia Dourados, Itahum, km 12, P.O. Box 533, 79.804-970 Dourados, MS, Brazil.
authors.forename: Arquimedes
authors.initials: A
authors.lastname: Gasparotto Junior
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