title: Adenosine receptor expression in a rat model of experimental autoimmune myasthenia gravis.
abstract: Extracellular adenosine is an essential negative regulator of immune reactions that acts by signaling via 4 distinct adenosine receptors. We evaluated adenosine receptor expression in Lewis rats presenting with experimental autoimmune myasthenia gravis (EAMG) to determine whether the expression of adenosine receptors are changed in the development and progression of EAMG. Lymphocyte A1AR and A2AAR mRNA and protein levels from lymphocytes harvested from the lymph nodes, spleen, and peripheral blood mononuclear cells (PBMCs) of EAMG rats were decreased. A modest but not significant increase in A2BAR levels was observed in EAMG lymphocytes harvested from lymph nodes and PBMCs. No changes in A3AR expression were observed in lymphocytes harvested from lymph nodes, spleen, or PBMCs following EAMG induction. Results presented in this report showed that the expression levels and the distribution pattern of adenosine receptors were altered in EAMG lymphocytes.
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China; Department of Histology and Embryology, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Anatomy, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Geng
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Yao
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Qingfei
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Shang
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Kang
authors.affiliation: Department of Histology and Embryology, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Lianhong
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Guangyou
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China.
authors.forename: Hulun
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China. Electronic address: mulili1982@aliyun.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Mu
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin Medical University, Heilongjiang 150081, China. Electronic address: sunbo720@aliyun.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D060888:Real-Time Polymerase Chain Reaction; D018047:Receptors, Purinergic P1 / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D001323:Autoantibodies; D018047:Receptors, Purinergic P1
keywords: AChR; Adenosine receptor; Autoimmune disease; EAMG