title: Fluidized bed layer-by-layer microcapsule formation.
abstract: Polymer microcapsules can be used as bioreactors and artificial cells; however, preparation methods for cell-like microcapsules are typically time-consuming, low yielding, and/or involve custom microfluidics. Here, we introduce a rapid (∼30 min per batch, eight layers), scalable (up to 500 mg of templates), and efficient (98% yield) microcapsule preparation technique utilizing a fluidized bed for the layer-by-layer (LbL) assembly of polymers, and we investigate the parameters that govern the formation of robust capsules. Fluidization in water was possible for particles of comparable diameter to mammalian cells (>5 μm), with the experimental flow rates necessary for fluidization matching well with the theoretical values. Important variables for polymer film deposition and capsule formation were the concentration of polymer solution and the molecular weight of the polymer, while the volume of the polymer solution had a negligible impact. In combination, increasing the polymer molecular weight and polymer solution concentration resulted in improved film deposition and the formation of robust microcapsules. The resultant polymer microcapsules had a thickness of ∼5.5 nm per bilayer, which is in close agreement with conventionally prepared (quiescent (nonflow) adsorption/centrifugation/wash) LbL capsules. The technique reported herein provides a new way to rapidly generate microcapsules (approximately 8 times quicker than the conventional means), while being also amenable to scale-up and mass production.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Richardson
authors.forename: Darwin
authors.initials: D
authors.lastname: Teng
authors.forename: Mattias
authors.initials: M
authors.lastname: Björnmalm
authors.forename: Sylvia T
authors.initials: ST
authors.lastname: Gunawan
authors.forename: Junling
authors.initials: J
authors.lastname: Guo
authors.forename: Jiwei
authors.initials: J
authors.lastname: Cui
authors.forename: George V
authors.initials: GV
authors.lastname: Franks
authors.forename: Frank
authors.initials: F
authors.lastname: Caruso
mesh_terms: D000327:Adsorption; D002214:Capsules / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D002214:Capsules; D011108:Polymers