title: Downregulated protein O-fucosyl transferase 1 (Pofut1) expression exerts antiproliferative and antiadhesive effects on hepatocytes by inhibiting Notch signalling.
abstract: The Notch signalling pathway is essential for proper cell growth and development. Many factors interact in the cellular context to bring about its effects. Critical to this process is the influence of Pofut1 whose function is to fucosylate Notch receptors and ligands on the cell surface for proper interaction. Of the three liver cell lines, HepG2, SMMC-7721 (hepatoma cell lines) and L-02 (normal fetal liver cell line) were investigated. In the current study, Pofut1 was silenced in L-02 due to its significantly high level of expression, (P<0.05) using transient SiRNA. Notch1, Cyclin D1 and p53 were significantly suppressed consequently. The effect on cell cycle, proliferation, adhesion and migration were investigated. Evidence adduced, indicate a general modification of cellular function. While proliferation and adhesion were significantly inhibited, the cell cycle arrest was obvious (P<0.05), migration was not affected. The effects seen are akin to those reported in previous studies in hepatoma cells mimicking some of the effects of notch1 silencing. Results of this study indicate a possible role of Pofut1 conferring a tumor suppressor role through the Notch signalling pathway.
authors.affiliation: Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road Western Section, Dalian 116044, Liaoning, China.
authors.forename: Max Efui
authors.initials: ME
authors.lastname: Annani-Akollor
authors.affiliation: Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road Western Section, Dalian 116044, Liaoning, China.
authors.forename: Shujing
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road Western Section, Dalian 116044, Liaoning, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road Western Section, Dalian 116044, Liaoning, China.
authors.forename: Linhua
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road Western Section, Dalian 116044, Liaoning, China.
authors.forename: Arshad Ahmed
authors.initials: AA
authors.lastname: Padhiar
authors.affiliation: Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road Western Section, Dalian 116044, Liaoning, China; School of Life Science and Medicine, Dalian University of Technology, 2 Linggong Road, Dalian 116024, China. Electronic address: jnzhang@dlmedu.edu.cn.
authors.forename: Jianing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology*; D015536:Down-Regulation / Q000502:physiology*; D005647:Fucosyltransferases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D020868:Gene Silencing; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D051881:Receptor, Notch1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C497761:NOTCH1 protein, human; D051881:Receptor, Notch1; D005647:Fucosyltransferases; C105148:polypeptide fucosyltransferase
keywords: EGF; Notch1; Pofut1