title: Sequence analysis of the complete mitochondrial genome of Youxian sheldrake.
abstract: Youxian sheldrake is excellent native breeds in Hunan province in China. The complete mitochondrial (mt) genome sequence plays an important role in the accurate determination of phylogenetic relationships among metazoans. This is the first study to determine the complete mitochondrial genome sequence of Youxian sheldrake using PCR-based amplification and Sanger sequencing. The characteristic of the entire mitochondrial genome was analyzed in detail, the total length of the mitogenome is 16,605 bp, with the base composition of 29.21% A, 22.18% T, 32.84% C, 15.77% G in the Youxian sheldrake. It contained 2 ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes and a major non-coding control region (D-loop region). The complete mitochondrial genome sequence of Youxian sheldrake provided an important data for further study of the phylogenetics of poultry, and available data for the genetics and breeding.
authors.affiliation: a College of Animal Science and Technology, Hunan Agricultural University , Changsha , P.R. China and.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: He
authors.affiliation: a College of Animal Science and Technology, Hunan Agricultural University , Changsha , P.R. China and.|b College of Life Science, Hunan University of Science and Technology , Xiangtan , P.R. China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Liu
authors.affiliation: a College of Animal Science and Technology, Hunan Agricultural University , Changsha , P.R. China and.
authors.forename: Qi-Fang
authors.initials: QF
authors.lastname: Yu
authors.affiliation: a College of Animal Science and Technology, Hunan Agricultural University , Changsha , P.R. China and.
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.affiliation: a College of Animal Science and Technology, Hunan Agricultural University , Changsha , P.R. China and.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: He
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D002681:China; D018387:Codon, Initiator / Q000235:genetics; D018388:Codon, Terminator / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D004372:Ducks / Q000235:genetics*; D059646:Genome Size / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary*
chemical_list: D018387:Codon, Initiator; D018388:Codon, Terminator; D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Genome; PCR method; Youxian sheldrake; mitochondrial