title: The Drosophila F-box protein Fbxl7 binds to the protocadherin fat and regulates Dachs localization and Hippo signaling.
abstract: The Drosophila protocadherin Fat (Ft) regulates growth, planar cell polarity (PCP) and proximodistal patterning. A key downstream component of Ft signaling is the atypical myosin Dachs (D). Multiple regions of the intracellular domain of Ft have been implicated in regulating growth and PCP but how Ft regulates D is not known. Mutations in Fbxl7, which encodes an F-box protein, result in tissue overgrowth and abnormalities in proximodistal patterning that phenocopy deleting a specific portion of the intracellular domain (ICD) of Ft that regulates both growth and PCP. Fbxl7 binds to this same portion of the Ft ICD, co-localizes with Ft to the proximal edge of cells and regulates the levels and asymmetry of D at the apical membrane. Fbxl7 can also regulate the trafficking of proteins between the apical membrane and intracellular vesicles. Thus Fbxl7 functions in a subset of pathways downstream of Ft and links Ft to D localization.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors.forename: Justin A
authors.initials: JA
authors.lastname: Bosch
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors.forename: Taryn M
authors.initials: TM
authors.lastname: Sumabat
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors.forename: Yassi
authors.initials: Y
authors.lastname: Hafezi
authors.affiliation: Department of Biology, Providence College, Providence, United States.
authors.forename: Brett J
authors.initials: BJ
authors.lastname: Pellock
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Gandhi
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States ikh@berkeley.edu.
authors.forename: Iswar K
authors.initials: IK
authors.lastname: Hariharan
mesh_terms: D000818:Animals; D001665:Binding Sites; D019521:Body Patterning / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D016764:Cell Polarity / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D015398:Signal Transduction; D022161:Transport Vesicles; D000099317:Hippo Kinases
chemical_list: D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; D044783:F-Box Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009218:Myosins; D017346:Protein Serine-Threonine Kinases; C000597200:Fbxl7 protein, Drosophila; C492999:dachs protein, Drosophila; C472307:ft protein, Drosophila; C499474:hpo protein, Drosophila; D000099317:Hippo Kinases
keywords: Hippo pathway; cadherin; cell proliferation; planar cell polarity; ubiquitin; vesicle
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