title: Two novel toxin variants revealed by whole-genome sequencing of 175 Clostridium botulinum type E strains.
abstract: We sequenced 175 Clostridium botulinum type E strains isolated from food, clinical, and environmental sources from northern Canada and analyzed their botulinum neurotoxin (bont) coding sequences (CDSs). In addition to bont/E1 and bont/E3 variant types, neurotoxin sequence analysis identified two novel BoNT type E variants termed E10 and E11. Strains producing type E10 were found along the eastern coastlines of Hudson Bay and the shores of Ungava Bay, while strains producing type E11 were only found in the Koksoak River region of Nunavik. Strains producing BoNT/E3 were widespread throughout northern Canada, with the exception of the coast of eastern Hudson Bay.
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: K A
authors.initials: KA
authors.lastname: Weedmark
authors.affiliation: Bureau of Microbial Hazards, Health Products and Food Branch, Health Canada, Ottawa, Ontario, Canada.
authors.forename: D L
authors.initials: DL
authors.lastname: Lambert
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Mabon
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: K L
authors.initials: KL
authors.lastname: Hayden
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: C J
authors.initials: CJ
authors.lastname: Urfano
authors.affiliation: Bureau of Microbial Hazards, Health Products and Food Branch, Health Canada, Ottawa, Ontario, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Leclair
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Van Domselaar
authors.affiliation: Bureau of Microbial Hazards, Health Products and Food Branch, Health Canada, Ottawa, Ontario, Canada.
authors.forename: J W
authors.initials: JW
authors.lastname: Austin
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada cindi.corbett@phac-aspc.gc.ca.
authors.forename: C R
authors.initials: CR
authors.lastname: Corbett
mesh_terms: D000818:Animals; D001905:Botulinum Toxins / Q000235:genetics*; D002170:Canada; D047970:Clostridium botulinum type E / Q000235:genetics*; D014644:Genetic Variation; D016680:Genome, Bacterial; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D001905:Botulinum Toxins
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