title: Factors associated with different results of allergy tests in children with dust mite-induced atopic dermatitis.
abstract: BACKGROUND
  Atopic dermatitis (AD) is a public health problem, with an increasing prevalence worldwide. AD is a chronic inflammatory disease characterised by skin lesions and severe itching. Immunologically, AD has two forms, IgE-mediated and cell-mediated, but it may also be idiopathic. In the pathogenesis of AD, the gene mutations for filaggrin, a filament-aggregating protein present in the epidermis, are of pivotal importance, but other genetic factors are also operating, including those linked to family atopy.
  
  
  METHODS
  We evaluated the role of family atopy, and of the results of the atopy patch test (APT) in parents, in children with mite-induced AD. 64 children, 38 males and 26 females, mean age 4.97 years, were included for the diagnosis of AD and underwent APT and skin prick test (SPT) with dust mite extracts, with evaluation of atopy and result of APT also in parents.
  
  
  RESULTS
  A positive family history of atopy was shown for children with positivity to both APT and SPT compared to those with negative or only one positive result to APT or SPT (p=0.08). Significant associations were found concerning APT results in children and parents. In particular, children of a positive-APT parent had an 18-fold higher risk of APT-positivity in comparison with children of negative-APT parents, while the risk was 6.6-fold higher if APT was positive in father.
  
  
  CONCLUSIONS
  Family atopy and a positive APT in fathers are risk factors to develop cell-mediated AD, as assessed by the APT, in children.
authors.affiliation: Pediatric Allergy Service, ASL FG, Torremaggiore, Italy. Electronic address: fuiano50@tin.it.
authors.forename: N
authors.initials: N
authors.lastname: Fuiano
authors.affiliation: Pediatrics Unit, IRCCS Casa Sollievo della Sofferenza Hospital, San Giovanni Rotondo, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Delvecchio
authors.affiliation: Allergy/Pulmonary Rehabilitation, ICP Hospital, Milan, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Incorvaia
mesh_terms: D000293:Adolescent; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000175:diagnosis / Q000276:immunology*; D005220:Fathers; D005260:Female; D000091344:Filaggrin Proteins; D006801:Humans; D007111:Immunity, Cellular; D007073:Immunoglobulin E / Q000097:blood; D007223:Infant; D008297:Male; D010290:Parents; D039981:Pyroglyphidae / Q000276:immunology; D012306:Risk; D012882:Skin Tests
chemical_list: D039741:Antigens, Dermatophagoides; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007073:Immunoglobulin E
keywords: Atopic dermatitis; Atopy patch test; Children; Family atopy; Genetics; House dust mite; Parents; Pathophysiology; Skin prick test