title: Glomerulonephritis Induced by Heterologous Anti-GBM Globulin as a Planted Foreign Antigen.
abstract: The glomerulonephritides are diseases characterized by immune-mediated glomerular inflammation. Most severe and rapidly progressive forms of glomerulonephritis feature the participation of injurious leukocytes that localize to glomeruli. This unit describes classical models of rapidly progressive glomerulonephritis in mice, induced by injecting heterologous globulin (raised in sheep) that binds to the glomerular basement membrane. These models have been particularly useful in defining the participation of effector leukocytes in severe glomerular disease. In these models, injury typically occurs in two phases. In the initial, heterologous phase, injury is mediated by the globulin bound within the glomerulus acting as an antibody. The later, autologous phase of injury is mediated by the host's adaptive immunity to the heterologous globulin now functioning as a planted foreign antigen within glomeruli. As autologous phase injury is driven by immunity to sheep globulin, assessment of antigen-specific systemic immunity to sheep globulin is critical when using this model.
authors.affiliation: Centre for Inflammatory Diseases, Monash University Department of Medicine, Clayton, Australia.|These authors contributed equally to this work.
authors.forename: Dragana
authors.initials: D
authors.lastname: Odobasic
authors.affiliation: Centre for Inflammatory Diseases, Monash University Department of Medicine, Clayton, Australia.|These authors contributed equally to this work.
authors.forename: Joanna R
authors.initials: JR
authors.lastname: Ghali
authors.affiliation: Centre for Inflammatory Diseases, Monash University Department of Medicine, Clayton, Australia.
authors.forename: Kim M
authors.initials: KM
authors.lastname: O'Sullivan
authors.affiliation: Centre for Inflammatory Diseases, Monash University Department of Medicine, Clayton, Australia.|Department of Nephrology, Monash Health, Clayton, Australia.
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Holdsworth
authors.affiliation: Centre for Inflammatory Diseases, Monash University Department of Medicine, Clayton, Australia.|Department of Nephrology, Monash Health, Clayton, Australia.|Department of Paediatric Nephrology, Monash Health, Clayton, Australia.
authors.forename: A Richard
authors.initials: AR
authors.lastname: Kitching
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005916:Globulins / Q000009:adverse effects* / Q000276:immunology / Q000494:pharmacology*; D050533:Glomerular Basement Membrane / Q000276:immunology / Q000473:pathology; D005921:Glomerulonephritis / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D006801:Humans; D051379:Mice; D012756:Sheep
chemical_list: D005916:Globulins
keywords: T lymphocytes; cell-mediated immunity; glomerulonephritis; planted antigen
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