title: Detection of rare antigen-presenting cells through T cell-intrinsic meandering motility, mediated by Myo1g.
abstract: To mount an immune response, T lymphocytes must successfully search for foreign material bound to the surface of antigen-presenting cells. How T cells optimize their chances of encountering and responding to these antigens is unknown. T cell motility in tissues resembles a random or Levy walk and is regulated in part by external factors including chemokines and lymph-node topology, but motility parameters such as speed and propensity to turn may also be cell intrinsic. Here we found that the unconventional myosin 1g (Myo1g) motor generates membrane tension, enforces cell-intrinsic meandering search, and enhances T-DC interactions during lymph-node surveillance. Increased turning and meandering motility, as opposed to ballistic motility, is enhanced by Myo1g. Myo1g acts as a "turning motor" and generates a form of cellular "flânerie." Modeling and antigen challenges show that these intrinsically programmed elements of motility search are critical for the detection of rare cognate antigen-presenting cells.
authors.affiliation: Department of Pathology, University of California, San Francisco, 513 Parnassus Avenue, HSW512, San Francisco, CA 94143-0511, USA.
authors.forename: Audrey
authors.initials: A
authors.lastname: Gérard
authors.affiliation: Experimental Immunology Branch National Cancer Institute, Bethesda, MD 20892-1360, USA.
authors.forename: Genaro
authors.initials: G
authors.lastname: Patino-Lopez
authors.affiliation: Department of Pathology, University of California, San Francisco, 513 Parnassus Avenue, HSW512, San Francisco, CA 94143-0511, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Beemiller
authors.affiliation: Cell and Developmental Biology Department, Vanderbilt University School of Medicine, Nashville, TN 37205, USA.
authors.forename: Rajalakshmi
authors.initials: R
authors.lastname: Nambiar
authors.affiliation: Experimental Immunology Branch National Cancer Institute, Bethesda, MD 20892-1360, USA.
authors.forename: Khadija
authors.initials: K
authors.lastname: Ben-Aissa
authors.affiliation: Experimental Immunology Branch National Cancer Institute, Bethesda, MD 20892-1360, USA.
authors.forename: Yin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathology, University of California, San Francisco, 513 Parnassus Avenue, HSW512, San Francisco, CA 94143-0511, USA.
authors.forename: Fadi J
authors.initials: FJ
authors.lastname: Totah
authors.affiliation: Cell and Developmental Biology Department, Vanderbilt University School of Medicine, Nashville, TN 37205, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Tyska
authors.affiliation: Experimental Immunology Branch National Cancer Institute, Bethesda, MD 20892-1360, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Shaw
authors.affiliation: Department of Pathology, University of California, San Francisco, 513 Parnassus Avenue, HSW512, San Francisco, CA 94143-0511, USA. Electronic address: matthew.krummel@ucsf.edu.
authors.forename: Matthew F
authors.initials: MF
authors.lastname: Krummel
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D007157:Immunologic Surveillance; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D015778:Minor Histocompatibility Antigens; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism
chemical_list: D015778:Minor Histocompatibility Antigens; C588241:Myo1g protein, mouse; D011948:Receptors, Antigen, T-Cell; D009218:Myosins
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