title: Noninvasive and quantitative assessment of in vivo fetomaternal interface angiogenesis using RGD-based fluorescence.
abstract: Angiogenesis is a key process for proper placental development and for the success of pregnancy. Although numerous in vitro methods have been developed for the assessment of this process, relatively few reliable in vivo methods are available to evaluate this activity throughout gestation. Here we report an in vivo technique that specifically measures placental neovascularization. The technique is based on the measurement of a fluorescent alpha v beta 3 (αvβ3) integrin-targeting molecule called Angiolone-Alexa-Fluor 700. The αvβ3 integrin is highly expressed by endothelial cells during the neovascularization and by trophoblast cells during their invasion of the maternal decidua. Angiolone was injected to gravid mice at 6.5 and 11.5 days post coitus (dpc). The fluorescence was analyzed one day later at 7.5 and 12.5 dpc, respectively. We demonstrated that (i) Angiolone targets αvβ3 protein in the placenta with a strong specificity, (ii) this technique is quantitative as the measurement was correlated to the increase of the placental size observed with increasing gestational age, and (iii) information on the outcome is possible, as abnormal placentation could be detected early on during gestation. In conclusion, we report the validation of a new noninvasive and quantitative method to assess the placental angiogenic activity, in vivo.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 823, Institut Albert Bonniot, Rond-point de la Chantourne, 38700 La Tronche, France ; Université Grenoble Alpes, 38041 Grenoble, France.
authors.forename: M
authors.initials: M
authors.lastname: Keramidas
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 823, Institut Albert Bonniot, Rond-point de la Chantourne, 38700 La Tronche, France ; Université Grenoble Alpes, 38041 Grenoble, France.
authors.forename: J
authors.initials: J
authors.lastname: Lavaud
authors.affiliation: Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Institut de Recherches en Sciences et Technologies pour le Vivant (iRTSV), Biologie du Cancer et de l'Infection, 17 rue des Martyrs, 38054 Grenoble, France.
authors.forename: F
authors.initials: F
authors.lastname: Sergent
authors.affiliation: Université Grenoble Alpes, 38041 Grenoble, France ; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 1036, Biologie du Cancer et de l'Infection, 17 rue des Martyrs, 38054 Grenoble, France ; CHU de Grenoble, Hôpital Couple Enfant, Département de Génétique et Procréation, Centre d'Aide Médicale à la Procréation, BP 217, 38043 Grenoble, France.
authors.forename: P
authors.initials: P
authors.lastname: Hoffmann
authors.affiliation: Université Grenoble Alpes, 38041 Grenoble, France ; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 1036, Biologie du Cancer et de l'Infection, 17 rue des Martyrs, 38054 Grenoble, France ; CHU de Grenoble, Hôpital Couple Enfant, Département de Génétique et Procréation, Centre d'Aide Médicale à la Procréation, BP 217, 38043 Grenoble, France.
authors.forename: S
authors.initials: S
authors.lastname: Brouillet
authors.affiliation: Université Grenoble Alpes, 38041 Grenoble, France ; Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Institut de Recherches en Sciences et Technologies pour le Vivant (iRTSV), Biologie du Cancer et de l'Infection, 17 rue des Martyrs, 38054 Grenoble, France ; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 1036, Biologie du Cancer et de l'Infection, 17 rue des Martyrs, 38054 Grenoble, France.
authors.forename: J-J
authors.initials: JJ
authors.lastname: Feige
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 823, Institut Albert Bonniot, Rond-point de la Chantourne, 38700 La Tronche, France ; Université Grenoble Alpes, 38041 Grenoble, France.
authors.forename: J-L
authors.initials: JL
authors.lastname: Coll
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 823, Institut Albert Bonniot, Rond-point de la Chantourne, 38700 La Tronche, France ; Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 1036, Biologie du Cancer et de l'Infection, 17 rue des Martyrs, 38054 Grenoble, France ; CHU de Grenoble, Hôpital Couple Enfant, Département de Génétique et Procréation, Centre d'Aide Médicale à la Procréation, BP 217, 38043 Grenoble, France.
authors.forename: N
authors.initials: N
authors.lastname: Alfaidy
mesh_terms: D000818:Animals; D001681:Biological Assay; D005260:Female; D005453:Fluorescence; D021621:Imaging, Three-Dimensional; D008431:Maternal-Fetal Exchange; D051379:Mice; D018919:Neovascularization, Physiologic; D009842:Oligopeptides / Q000378:metabolism*; D010920:Placenta / Q000002:abnormalities / Q000378:metabolism; D011247:Pregnancy
chemical_list: D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid
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