title: Co-Administration of Metformin and N-Acetyl Cysteine Fails to Improve Clinical Manifestations in PCOS Individual Undergoing ICSI.
abstract: BACKGROUND
  Studies have demonstrated the efficacy of metformin (MTF ) in reducing insulin resistance and N-acetyl cysteine (NAC) in inhibiting oxidative stress which are involved in the pathogenesis of polycystic ovarian syndrome (PCOS). We aimed to compare the effects of MTF and NAC combination on serum metabolite and hormonal levels during the course of ovulation induction in PCOS individual candidates of intracytoplasmic sperm injection (ICSI).
  
  
  METHODS
  In this prospective randomized clinical trial, placebo con- trolled pilot study, 80 patients of polycystic ovarian syndrome at the age of 25-35 years were divided into 4 groups (n=20): i. NAC=treated with N-acetyl cysteine (600 mg three times daily), ii. MTF=treated with metformin (500 mg three times daily), iii. MTF+NAC=treated with N-acetyl cysteine plus metformin (the offered doses) and iv. placebo (PLA). A total number of 20 patients (6 from MTF group, 4 from NAC group, 6 from MTF+NAC group and 4 from PLA group) were dropped of the study. The drugs were administrated from day 3 of menses of previous cycle until ovum pick-up.
  
  
  RESULTS
  Serum levels of luteinizing hormone (LH), total testosterone, cholester- ol and triglyceride, insulin and leptin significantly reduced in the MTF and NAC groups compared to the placebo (p<0.01). But levels of LH, total testosterone, cholesterol and triglyceride had no significant reduction in the MTF+NAC groups compared to the placebo. The serum levels of malonyldialdehyde (MDA), insulin and leptin reduced significantly after treatment in the MTF+NAC group compared to the placebo (p<0.05).
  
  
  CONCLUSIONS
  CONSIDERING THE ADVERSE EFFECT OF COMBINATION THERAPY, WE PROPOSED THE CONADMINISTRATION MIGHT HAVE NO BENEFICIAL EFFECT FOR PCOS PATIENT DURING COURSE OF OVULATION INDUCTION OF ICSI (REGISTRATION NUMBER: IRCT201204159476N1).
authors.affiliation: Medical Sciences Research Laboratory, Department of Biology, Faculty of Science, Arak University, Arak, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Cheraghi
authors.affiliation: Department of Biology, Faculty of Science, Arak University, Arak, Iran.
authors.forename: Malek
authors.initials: M
authors.lastname: Soleimani Mehranjani
authors.affiliation: Department of Biology, Faculty of Science, Arak University, Arak, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shariatzadeh
authors.affiliation: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute, ACECR, Isfahan, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nasr Esfahani
authors.affiliation: Obstetrics and Gynecology Unit, Infertility Treatment Center, ACECR, Qom, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Ebrahimi
keywords: Insulin Resistance; Metformin; N-Acetyl Cysteine; Oxidative Stress; Polycystic Ovary Syndrome
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