title: Improvement of Wuyiencin biosynthesis in Streptomyces wuyiensis CK-15 by identification of a key regulator, WysR.
abstract: Wuyiencin is produced by Streptomyces ahygroscopicus var. wuyiensis CK-15 and is widely used as an antifungal agent in agriculture. Analysis of wuyiencin biosynthetic gene clusters reveals wysR, a member of the LAL-family of transcriptional regulatory genes. WysR consists of an Nterminal PAS domain and a LuxR family C-terminal helix-turn-helix motif. However, the roles of wysR in wuyiencin biosynthesis are largely unknown. In this study, we showed that inactivation of wysR resulted in the complete loss of wuyiencin production, which could be restored by complementation with a single copy of wysR. Furthermore, we successfully increased wuyiencin production to a significantly higher level by overexpression of wysR in S. wuyiensis CK-15. Quantitative real-time RT-PCR analysis showed that WysR regulates wuyiencin biosynthesis by modulating other putative regulatory genes. Thus, WysR was identified as an activator of wuyiencin biosynthesis, and overexpression of wysR gene proved to be an effective strategy for improving wuyiencin production.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hojin
authors.initials: H
authors.lastname: Ryu
authors.forename: Beibei
authors.initials: B
authors.lastname: Ge
authors.forename: Guohui
authors.initials: G
authors.lastname: Pan
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Kyungseok
authors.initials: K
authors.lastname: Park
authors.forename: Kecheng
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D060847:Metabolic Engineering; D060888:Real-Time Polymerase Chain Reaction; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D014157:Transcription Factors