title: Single-nucleotide polymorphism analysis of GH, GHR, and IGF-1 genes in minipigs.
abstract: Tibetan (TB) and Bama (BM) miniature pigs are two popular pig breeds that are used as experimental animals in China due to their small body size. Here, we analyzed single-nucleotide polymorphisms (SNPs) in gene fragments that are closely related to growth traits [growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor (IGF)-1)] in these pig breeds and a large white (LW) control pig breed. On the basis of the analysis of 100 BMs, 108 TBs, and 50 LWs, the polymorphic distribution levels of GH, GHR, and IGF-1 were significantly different among these three pig breeds. According to correlation analyses between SNPs and five growth traits--body weight (BW), body length (BL), withers height (WH), chest circumference (CC), and abdomen circumference (AC)--three SNP loci in BMs and four SNP loci in TBs significantly affected growth traits. Three SNP sites in BMs and four SNP sites in TBs significantly affected growth traits. SNPs located in the GH gene fragment significantly affected BL and CC at locus 12 and BL at locus 45 in BMs, and also BW, WH, CC, and AC at locus 45 and WH and CC at locus 93 in TBs. One SNP at locus 85 in the BM GHR gene fragment significantly affected all growth traits. All indices were significantly reduced with a mixture of alleles at locus 85. These results provide more information regarding the genetic background of these minipig species and indicate useful selection markers for pig breeding programs.
authors.affiliation: Laboratory Animal Center, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Y G
authors.initials: YG
authors.lastname: Tian
authors.affiliation: Laboratory Animal Center, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: M
authors.initials: M
authors.lastname: Yue
authors.affiliation: Laboratory Animal Center, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Laboratory Animal Center, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: W W
authors.initials: WW
authors.lastname: Gu
authors.affiliation: Laboratory Animal Center, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D000483:Alleles; D000818:Animals; D049628:Body Size; D004247:DNA / Q000302:isolation & purification; D004392:Dwarfism / Q000235:genetics; D056426:Genetic Loci; D005838:Genotype; D013006:Growth Hormone / Q000235:genetics*; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000502:physiology*; D011986:Receptors, Somatotropin / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013552:Swine; D013556:Swine, Miniature / Q000235:genetics*
chemical_list: D011986:Receptors, Somatotropin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D004247:DNA
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