title: Differential expression of a retrotransposable element,Rex6, inColossoma macropomumfish from different Amazonian environments.
abstract: Transposable elements (TEs) are DNA sequences that have the ability to move and replicate within the genomes. TEs can be classified according to their intermediates of transposition, RNA (retrotransposons) or DNA. In some aquatic organisms, it has been observed that environmental factors such as pH, temperature and pollution may stimulate differential transcription and mobilization of retrotransposons. In light of this information, the present study sought to evaluate the expression ofRex6TE transcripts inColossoma macropomum, which is a very commercially exploited fish in Brazil. In order to establish a comparative analysis using real-time PCR, the samples were collected from Amazonian rivers with different physical and chemical characteristics (distinguished by clear water and black water). Quantitative RT-PCR analyses revealed a differential pattern of expression between tissues collected from different types of water (clear and black waters). When it came to the hepatic and muscle tissues sampled, the levels ofRex6transcripts were significantly different between the two Amazonian water types. These results suggest that environmental conditions operate differently in the regulation ofRex6transcription inC. macropomum,results which have implications in the reshaping of the genome against environmental variations.
authors.affiliation: UNESP-São Paulo State University (Universidade Estadual Paulista); Instituto de Biociências de Botucatu; Departamento de Morfologia; Botucatu, SP, Brazil.
authors.forename: Cassiane Martins
authors.initials: CM
authors.lastname: Barbosa
authors.affiliation: UNESP-São Paulo State University (Universidade Estadual Paulista); Instituto de Biociências de Botucatu; Departamento de Morfologia; Botucatu, SP, Brazil.
authors.forename: Edson Assunção
authors.initials: EA
authors.lastname: Mareco
authors.affiliation: UNESP-São Paulo State University (Universidade Estadual Paulista); Instituto de Biociências de Botucatu; Departamento de Morfologia; Botucatu, SP, Brazil.
authors.forename: Maeli Dal Pai
authors.initials: MD
authors.lastname: Silva
authors.affiliation: UNESP-São Paulo State University (Universidade Estadual Paulista); Instituto de Biociências de Botucatu; Departamento de Morfologia; Botucatu, SP, Brazil.
authors.forename: Cesar
authors.initials: C
authors.lastname: Martins
authors.affiliation: UNIP-University of São Paulo (Universidade Paulista); Instituto de Ciências da Saúde; Bauru, SP, Brazil.
authors.forename: Fernanda Antunes
authors.initials: FA
authors.lastname: Alves-Costa
keywords: differential expression; quantitative RT-PCR; transcript tissue distribution; transposable elements
references.citation: Mol Biol Evol. 2000 Nov;17(11):1673-84
references.pmid: 11070055
references.citation: Genome Res. 2003 Jul;13(7):1686-95
references.pmid: 12805276
references.citation: Genetica. 1994;93(1-3):149-60
references.pmid: 7813912
references.citation: Mar Pollut Bull. 2004 Apr;48(7-8):705-10
references.pmid: 15041427
references.citation: Cytogenet Genome Res. 2010;129(4):314-22
references.pmid: 20606399
references.citation: Mol Biol Evol. 2001 Mar;18(3):427-31
references.pmid: 11230544
references.citation: Fish Shellfish Immunol. 2007 Nov;23(5):1072-88
references.pmid: 17613247
references.citation: Mar Environ Res. 2004 Jun;57(5):377-95
references.pmid: 14967520
references.citation: Biochem Biophys Res Commun. 1998 Dec 30;253(3):828-33
references.pmid: 9918813
references.citation: Int J Genomics. 2013;2013:465727
references.pmid: 24350238
references.citation: FASEB J. 2009 May;23(5):1482-9
references.pmid: 19141532
references.citation: Chromosome Res. 2008;16(1):203-15
references.pmid: 18293113
references.citation: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references.pmid: 7984417
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: Mol Biol Evol. 1999 Nov;16(11):1427-38
references.pmid: 10555274
references.citation: Cell Tissue Res. 2008 Mar;331(3):643-58
references.pmid: 18071755
references.citation: Cytogenet Genome Res. 2009;125(3):224-34
references.pmid: 19738382
references.citation: Environ Health Perspect. 2007 Sep;115(9):1264-70
references.pmid: 17805414
references.citation: Genetica. 2009 Jul;136(3):461-9
references.pmid: 19112556
references.citation: Annu Rev Genet. 2007;41:331-68
references.pmid: 18076328
references.citation: J Mol Evol. 2001 Apr;52(4):351-60
references.pmid: 11343131
references.citation: J Biotechnol. 2006 Jun 10;123(4):397-412
references.pmid: 16442657
references.citation: Heredity (Edinb). 2000 Aug;85 ( Pt 2):101-6
references.pmid: 11012710
references.citation: J Exp Biol. 1998 Sep 22;201(Pt 20):2801-2813
references.pmid: 9739063
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: J Mol Biol. 2005 Nov 25;354(2):246-57
references.pmid: 16249005