title: Maternal lipopolysaccharide alters the newborn oxidative stress and C-reactive protein levels in response to an inflammatory stress.
abstract: Maternal infection is associated with oxidative stress (OS) and inflammatory responses. We have previously shown that maternal exposure to lipopolysaccharide (LPS) at E18 alters the subsequent offspring immune response. As immune responses are mediated, in part, by OS, we sought to determine if maternal inflammation during pregnancy programs offspring OS and C-reactive protein (CRP) levels. Pregnant Sprague-Dawley rats received intraperitoneal (i.p.) injections of saline or LPS at 18 days' gestation (n = 4), and pups delivered spontaneously at term. At postnatal day 24, male and female offspring received i.p. injection of LPS. Serum lipid peroxides formation (PD) and CRP levels were determined before and at 4 h following the LPS injection. Pups of LPS-exposed dams had significantly higher basal OS (PD 29.4 ± 5.4 v. 10.1 ± 4.8 nmol/ml) compared with controls. In response to LPS, CRP levels (20.4 ± 2.8 v. 5.7 ± 1.0 ng/ml) were significantly higher among pups of LPS-exposed dams than controls. Prenatal maternal exposure to LPS increases baseline OS levels in neonates and CRP levels in response to LPS. These results suggest that maternal inflammation during the antenatal period may induce long-term sequelae in the offspring that may predispose to adult disease.
authors.affiliation: 1 Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Ginsberg
authors.affiliation: 1 Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
authors.forename: P
authors.initials: P
authors.lastname: Lotan
authors.affiliation: 1 Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Khatib
authors.affiliation: 1 Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Awad
authors.affiliation: 1 Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Errison
authors.affiliation: 1 Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
authors.forename: Z
authors.initials: Z
authors.lastname: Weiner
authors.affiliation: 1 Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Maravi
authors.affiliation: 2 Department of Obstetrics and Gynecology, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Ross
authors.affiliation: 1 Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
authors.forename: J
authors.initials: J
authors.lastname: Itskovitz-Eldor
authors.affiliation: 1 Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Beloosesky
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism*; D005260:Female; D015227:Lipid Peroxidation; D008070:Lipopolysaccharides / Q000276:immunology*; D018384:Oxidative Stress; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D017207:Rats, Sprague-Dawley
chemical_list: D008070:Lipopolysaccharides; D002097:C-Reactive Protein