title: Carboxybetaine methacrylate oligomer modified nylon for circulating tumor cells capture.
abstract: Circulating tumor cells (CTC) capture is one of the most effective approaches in diagnosis and treatment of cancers in the field of personalized cancer medicine. In our study, zwitterionic carboxybetaine methacrylate (CBMA) oligomers were grafted onto nylon via atomic transfer random polymerization (ATRP) which would serve as a novel material for the development of convenient CTC capture interventional medical devices. The chemical, physical and biological properties of pristine and modified nylon surfaces were assessed by Fourier transform infrared spectra, atomic force microscope, water contact angle measurements, X-ray photoelectron spectroscopy, protein adsorption, platelet adhesion, and plasma recalcification time (PRT) determinations, etc. The results, including the significant decrease of proteins adsorption and platelets adhesion, as well as prolonged PRTs demonstrated the extraordinary biocompatibility and blood compatibility of the modified surface. Furthermore, we showed that upon immobilization of anti-epithelial cell adhesion molecular (anti-EpCAM) antibody onto the CBMA moiety, the modified nylon surface can selectively capture EpCAM positive tumor cells from blood with high efficiency, indicating the potential of the modified nylon in the manufacture of convenient interventional CTC capture medical devices.
authors.affiliation: College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Dong
authors.affiliation: Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing 210008, China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Stony Brook University, Stony Brook, NY 11790, USA.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China. Electronic address: ztnj@nju.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University, Clinical Cancer Institute of Nanjing University, 321 Zhongshan Road, Nanjing 210008, China.
authors.forename: Baorui
authors.initials: B
authors.lastname: Liu
mesh_terms: D000912:Antibodies, Neoplasm / Q000737:chemistry*; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D000071858:Epithelial Cell Adhesion Molecule; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000378:metabolism; D009360:Neoplastic Cells, Circulating / Q000378:metabolism / Q000473:pathology; D009757:Nylons / Q000737:chemistry*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D008689:Methacrylates; D009363:Neoplasm Proteins; D009757:Nylons
keywords: ATRP; Capturing; Carboxybetaine methacrylate; Circulating tumor cells; Interventional medical devices; Nylon