title: The transcription factors Sox5 and Sox9 regulate Catsper1 gene expression.
abstract: Catsper is a Ca(2+)permeable channel required for sperm hyperactivation. In spite of its central role in male fertility, the transcriptional mechanisms that regulate Catsper1 expression are ill defined. In this work, we describe the identification and characterization of important regulatory elements in the murine Catsper1 gene proximal promoter. Four transcription start sites and three functional Sox-binding sites were identified in the Catsper1 promoter. Interestingly, transcription factors Sox5 and Sox9 caused a significant increase in transactivation of the Catsper1 promoter in heterologous systems, and chromatin immunoprecipitation assays showed that both transcription factors interact with the Catsper1 promoter in vivo. These results provide new insights into the molecular mechanisms that control Catsper channel expression.
authors.affiliation: Unidad de Investigación Médica en Enfermedades Metabólicas, Instituto Mexicano del Seguro Social (IMSS), México DF 06720, Mexico; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), México DF 07360, Mexico.
authors.forename: Minerva
authors.initials: M
authors.lastname: Mata-Rocha
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), México DF 07360, Mexico.
authors.forename: Javier
authors.initials: J
authors.lastname: Hernández-Sánchez
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), México DF 07360, Mexico.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Guarneros
authors.affiliation: Unidad de Investigación Médica en Enfermedades Metabólicas, Instituto Mexicano del Seguro Social (IMSS), México DF 06720, Mexico.
authors.forename: Elsa
authors.initials: E
authors.lastname: de la Chesnaye
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Cuernavaca 62210, Mexico.
authors.forename: Ana A
authors.initials: AA
authors.lastname: Sánchez-Tusié
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Cuernavaca 62210, Mexico.
authors.forename: Claudia L
authors.initials: CL
authors.lastname: Treviño
authors.affiliation: Departamento de Biología Celular, Cinvestav, México DF 07360, Mexico.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Felix
authors.affiliation: Unidad de Investigación Médica en Enfermedades Metabólicas, Instituto Mexicano del Seguro Social (IMSS), México DF 06720, Mexico. Electronic address: naoviedoa@yahoo.com.mx.
authors.forename: Norma
authors.initials: N
authors.lastname: Oviedo
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D055758:SOX9 Transcription Factor / Q000502:physiology*; D055755:SOXD Transcription Factors / Q000502:physiology*; D013087:Spermatids / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013737:Testis / Q000166:cytology; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D015220:Calcium Channels; C488642:Catsper1 protein, mouse; D055758:SOX9 Transcription Factor; D055755:SOXD Transcription Factors; C089186:Sox5 protein, mouse; C527933:Sox9 protein, mouse
keywords: Calcium channels; Catsper1; Sox5; Sox9; Transcriptional regulation