title: Identification of bacterial community composition in freshwater aquaculture system farming of Litopenaeus vannamei reveals distinct temperature-driven patterns.
abstract: Change in temperature is often a major environmental factor in triggering waterborne disease outbreaks. Previous research has revealed temporal and spatial patterns of bacterial population in several aquatic ecosystems. To date, very little information is available on aquaculture environment. Here, we assessed environmental temperature effects on bacterial community composition in freshwater aquaculture system farming of Litopenaeus vannamei (FASFL). Water samples were collected over a one-year period, and aquatic bacteria were characterized by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and 16S rDNA pyrosequencing. Resulting DGGE fingerprints revealed a specific and dynamic bacterial population structure with considerable variation over the seasonal change, suggesting that environmental temperature was a key driver of bacterial population in the FASFL. Pyrosequencing data further demonstrated substantial difference in bacterial community composition between the water at higher (WHT) and at lower (WLT) temperatures in the FASFL. Actinobacteria, Proteobacteria and Bacteroidetes were the highest abundant phyla in the FASFL, however, a large number of unclassified bacteria contributed the most to the observed variation in phylogenetic diversity. The WHT harbored remarkably higher diversity and richness in bacterial composition at genus and species levels when compared to the WLT. Some potential pathogenenic species were identified in both WHT and WLT, providing data in support of aquatic animal health management in the aquaculture industry.
authors.affiliation: Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China Ministry of Agriculture, College of Food Science and Technology, Shanghai Ocean University, 999 Hu Cheng Huan Road, Shanghai 201306, China. m110250462@st.shou.edu.cn.
authors.forename: Yuyi
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China Ministry of Agriculture, College of Food Science and Technology, Shanghai Ocean University, 999 Hu Cheng Huan Road, Shanghai 201306, China. tpy2011812@sina.com.
authors.forename: Peiying
authors.initials: P
authors.lastname: Tao
authors.affiliation: Shanghai Key Laboratory of Meteorology and Health, 951 Jinxiu Road, Shanghai 200135, China. jianguot@21cn.com.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Tan
authors.affiliation: Shanghai Key Laboratory of Meteorology and Health, 951 Jinxiu Road, Shanghai 200135, China. muhz@climate.sh.cn.
authors.forename: Haizhen
authors.initials: H
authors.lastname: Mu
authors.affiliation: Shanghai Key Laboratory of Meteorology and Health, 951 Jinxiu Road, Shanghai 200135, China. phyllis_pl@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Peng
authors.affiliation: Shanghai Key Laboratory of Meteorology and Health, 951 Jinxiu Road, Shanghai 200135, China. ydd_danfer@hotmail.com.
authors.forename: Dandan
authors.initials: D
authors.lastname: Yang
authors.affiliation: School of Public Health, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia Kelvin Grove, QLD 4059, Australia. s.tong@qut.edu.au.
authors.forename: Shilu
authors.initials: S
authors.lastname: Tong
authors.affiliation: Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China Ministry of Agriculture, College of Food Science and Technology, Shanghai Ocean University, 999 Hu Cheng Huan Road, Shanghai 201306, China. lmchen@shou.edu.cn.
authors.forename: Lanming
authors.initials: L
authors.lastname: Chen
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D017756:Aquaculture; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D058645:Denaturing Gradient Gel Electrophoresis; D005618:Fresh Water / Q000382:microbiology*; D033561:Penaeidae; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017423:Sequence Analysis, RNA; D013696:Temperature
chemical_list: D012336:RNA, Ribosomal, 16S
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