title: Expression, purification and crystallization of pecan (Carya illinoinensis) vicilin.
abstract: Tree nuts are responsible for many cases of severe food allergies. The 7S seed storage protein vicilin has been identified as a food allergen in many kinds of tree nuts. The vicilin protein consists of an N-terminal low-complexity region with antimicrobial activity and a C-terminal domain that forms a trimeric structure that belongs to the cupin superfamily. In this study, vicilin from pecan (Carya illinoinensis) was isolated and was expressed in bacteria for the first time. The cupin structural core of the protein, residues 369-792, was purified by metal-affinity and gel-filtration chromatography to high purity. Vicilin crystals were obtained and the best crystal diffracted to 2.65 Å resolution in space group P212121.
authors.affiliation: Healthy Processed Foods Research Unit, USDA-ARS-PWA, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
authors.forename: BoRam
authors.initials: B
authors.lastname: Lee
authors.affiliation: Healthy Processed Foods Research Unit, USDA-ARS-PWA, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
authors.forename: Renhao
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Healthy Processed Foods Research Unit, USDA-ARS-PWA, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
authors.forename: Wen-Xian
authors.initials: WX
authors.lastname: Du
authors.affiliation: Crop Germplasm Research, USDA-ARS-SPA, Southern Plains Agricultural Research Center, 2881 F&B Road, College Station, TX 778450, USA.
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Grauke
authors.affiliation: Healthy Processed Foods Research Unit, USDA-ARS-PWA, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
authors.forename: Tara H
authors.initials: TH
authors.lastname: McHugh
authors.affiliation: Healthy Processed Foods Research Unit, USDA-ARS-PWA, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
authors.forename: Yu-Zhu
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D031325:Carya / Q000737:chemistry*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003460:Crystallization; D017931:DNA Primers; D055314:Seed Storage Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D055314:Seed Storage Proteins; C015575:vicilin protein, plant
keywords: Carya illinoinensis; convicilin; food allergen; storage protein
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