title: Isolation and characterization of adipose-derived mesenchymal stem cells (ADSCs) from cattle.
abstract: Adipose-derived mesenchymal stem cells (ADSCs) were isolated from the adult adipose tissue of 2-year-old cattle, and then characterized by immunofluorescence and RT-PCR. We found that primary bADSCs could be expanded for 25 passages. Expression of β-integrin, CD44, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 bADSCs were successfully induced to differentiate into osteoblasts and adipocytes. The results indicate the potential for multi-lineage differentiation of bADSCs that may represent an ideal candidate for cellular transplantation therapy.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Taofeng
authors.initials: T
authors.lastname: Lu
authors.forename: Hui
authors.initials: H
authors.lastname: Xiong
authors.forename: Kunfu
authors.initials: K
authors.lastname: Wang
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Ma
authors.forename: Weijun
authors.initials: W
authors.lastname: Guan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001483:Base Sequence; D001842:Bone and Bones / Q000166:cytology; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division; D017931:DNA Primers; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers