title: Anti-ribosomal P antibodies are associated with elevated circulating IFNα and IL-10 levels in systemic lupus erythematosus patients.
abstract: OBJECTIVE
  The objective of this paper is to analyze the relationship of anti-protein ribosomal P (RibP) antibodies with circulating levels of IFNα, TNFα, IFNγ, IL-17 and IL-10 in SLE. Disease activity and other systemic lupus erythematosus (SLE) features were also analyzed.
  
  
  METHODS
  Anti-RibP and other SLE-related antinuclear antibodies (ANA) were determined by fluoro-enzyme immunoassay in the sera of 107 SLE patients. Circulating cytokines were quantified by flow cytometry (IFNα, IL-10 and IL-17) or ELISA (TNFα and IFNγ).
  
  
  RESULTS
  Anti-RibP-positive patients (14.9%) displayed significantly higher serum levels of IFNα (p = 0.023) and IL-10 (p = 0.016) than their negative counterparts. This cytokine upregulation was independent of the presence of other ANA even though, in our patient cohort, anti-dsDNA was found to be associated with anti-RibP (OR, CI 95%: 6.03, 1.32-27.93, p = 0.021) and to correlate with IL-10 levels (r = 0.204, p = 0.036). In fact, patients positive for anti-RibP but negative for anti-dsDNA exhibited the highest amounts of both IL-10 and IFN-α that were not related to disease activity since these patients showed lower SLEDAI than patients also positive for anti-dsDNA (p = 0.018). Anti-RibP positivity was also associated with early diagnosis, hypocomplementemia and leukopenia.
  
  
  CONCLUSIONS
  Presence of anti-RibP was found to be related to increased serum IFNα and IL-10 levels independently of both antibody status and disease activity.
authors.affiliation: Department of Immunology, Hospital Universitario Central de Asturias, Oviedo, Spain lourdes.mozo@sespa.princast.es.
authors.forename: L
authors.initials: L
authors.lastname: Mozo
authors.affiliation: Department of Functional Biology, Immunology Area, Universidad de Oviedo, Oviedo, Spain.
authors.forename: P
authors.initials: P
authors.lastname: López
authors.affiliation: Internal Medicine, Hospital Universitario Central de Asturias, Oviedo, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Caminal-Montero
authors.affiliation: Department of Functional Biology, Immunology Area, Universidad de Oviedo, Oviedo, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Rodríguez-Carrio
authors.affiliation: Department of Functional Biology, Immunology Area, Universidad de Oviedo, Oviedo, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Suárez
mesh_terms: D000328:Adult; D000974:Antibodies, Antinuclear; D001323:Autoantibodies / Q000097:blood*; D003165:Complement System Proteins / Q000378:metabolism; D004247:DNA / Q000276:immunology; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood*; D007970:Leukopenia / Q000097:blood; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D012269:Ribosomal Proteins / Q000276:immunology*; D012720:Severity of Illness Index
chemical_list: D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D016898:Interferon-alpha; D012269:Ribosomal Proteins; D016753:Interleukin-10; D003165:Complement System Proteins; D004247:DNA
keywords: Anti-ribosomal protein (RibP) antibodies; IFNα; IL-10; SLE; anti-dsDNA; disease activity