title: CYP19A1 single nucleotide polymorphism associations with CYP19A1, NFκB1, and IL6 gene expression in human normal colon and normal liver samples.
abstract: BACKGROUND
  Estrogen is known to decrease the risk of colon cancer in postmenopausal women, and may exert its actions by decreasing interleukin-6 (IL6) production via stabilization of the transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB). Estrogens are biosynthesized by CYP19A1 (aromatase), so it is possible that genetic variations in CYP19A1 influences the risk of colon cancer by altering expression of CYP19A1. Further, studies on gene-gene interactions suggest that single nucleotide polymorphisms in one gene may affect expression of other genes. The current study aims to explore the role of CYP19A1 single nucleotide polymorphisms on CYP19A1, NFκB1 and IL6 gene expression.
  
  
  METHODS
  Phenotype-genotype associations, cross-associations between genes, and haplotype analyses were performed in both normal human colon (n=82) and liver (n=238) samples.
  
  
  RESULTS
  CYP19A1 rs10459592, rs1961177, and rs6493497 were associated with CYP19A1 expression in colon samples (P=0.042, P=0.041, and P=0.013, respectively). CYP19A1 single nucleotide polymorphisms (rs12908960, rs730154, rs8025191, and rs17523880) were correlated with NFκB1 expression (P=0.047, P=0.04, P=0.05, and P=0.03, respectively), and CYP19A1 rs11856927, rs2470152, and rs2470144 (P=0.049, P=0.025, P=0.047, respectively) were associated with IL6 expression in the colon. While rs730154 and rs17523880 could not be analyzed in the liver samples, none of the other associations with the colon were replicated in the liver samples. Haplotype analysis revealed three separate haplotypes of the CYP19A1 single nucleotide polymorphism that were significantly associated with CYP19A1, NFκB1, and IL6 gene expression.
  
  
  CONCLUSIONS
  CYP19A1 single nucleotide polymorphisms are associated not only with CYP19A1 expression but also with NFκB1 and IL6 expression. These data demonstrate the possible functional consequences of genetic variation within the CYP19A1 gene on other genes in a biologically plausible pathway.
authors.affiliation: Department of Environmental and Occupational Health, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
authors.forename: Rosalind B
authors.initials: RB
authors.lastname: Penney
authors.affiliation: Department of Internal Medicine, University of Utah Health Sciences Center, Salt Lake City, UT, USA.
authors.forename: Abbie
authors.initials: A
authors.lastname: Lundgreen
authors.affiliation: Division of Medical Genetics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
authors.forename: Aiwei
authors.initials: A
authors.lastname: Yao-Borengasser
authors.affiliation: Division of Medical Genetics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
authors.forename: Vineetha K
authors.initials: VK
authors.lastname: Edavana
authors.affiliation: Division of Medical Genetics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Williams
authors.affiliation: Department of Biostatistics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
authors.forename: Ishwori
authors.initials: I
authors.lastname: Dhakal
authors.affiliation: Department of Internal Medicine, University of Utah Health Sciences Center, Salt Lake City, UT, USA.
authors.forename: Roger K
authors.initials: RK
authors.lastname: Wolff
authors.affiliation: Division of Medical Genetics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Kadlubar
authors.affiliation: Department of Internal Medicine, University of Utah Health Sciences Center, Salt Lake City, UT, USA.
authors.forename: Martha L
authors.initials: ML
authors.lastname: Slattery
keywords: CYP19A1; NFκB1; colon; interleukin-6; liver; single nucleotide polymorphism
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