title: Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique.
abstract: In this manuscript, we demonstrate the rapid formation of three-dimensional (3D) embryonic stem cell (ESC) aggregates with controllable sizes and shapes in hydrogels using dielectrophoresis (DEP). The ESCs encapsulated within a methacrylated gelatin (GelMA) prepolymer were introduced into a DEP device and, upon applying an electric field and crosslinking of the GelMA hydrogel, formed 3D ESC aggregates. Embryoid bodies (EBs) fabricated using this method showed high cellular viability and pluripotency. The proposed technique enables production of EBs on a large scale and in a high-throughput manner for potential cell therapy and tissue regeneration applications.
authors.affiliation: WPI-Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan. ramonazconjavier@gmail.com.
authors.forename: Samad
authors.initials: S
authors.lastname: Ahadian
authors.forename: Shukuyo
authors.initials: S
authors.lastname: Yamada
authors.forename: Javier
authors.initials: J
authors.lastname: Ramón-Azcón
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002470:Cell Survival; D004586:Electrophoresis / Q000379:methods*; D058732:Embryoid Bodies / Q000502:physiology*; D053595:Embryonic Stem Cells; D057166:High-Throughput Screening Assays / Q000379:methods*; D020100:Hydrogels / Q000737:chemistry*; D008954:Models, Biological
chemical_list: D020100:Hydrogels