title: Genetic diversity among monoconidial and polyconidial isolates of Bipolaris sorokiniana.
abstract: Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat in warm and humid wheat-growing regions of the world. This fungus shows a high genetic diversity and morphological and physiologic variability. In this study, 19 polysporic and 57 monosporic isolates of B. sorokiniana were characterized using universal rice primers-URP-PCR. The results obtained when the dendrogram was constructed with all the data produced with the amplification products showed very distinct clusters. However, the similarity among the isolates was low where 37 and 26.3 % of the monosporic and polysporic isolates, respectively, showed similarity above 70 %. All primers amplified multiple DNA fragments of polysporic as well as the monosporic isolates. Isolates fingerprints were constructed based on binary characters revealed by the three primers. An amplified fragment of approximately 750 bp was observed among 40 % of the isolates, when primer URP-1F was used. When primers URP-4R and URP-2R were used, a fragment of 450 and 400 bp was present in 31.5 and 29 % of the isolates, respectively. It was expected a higher similarity among the isolates since the monosporic cultures were originated from the polysporic. The dendrogram did not enable the separation of B. sorokiniana isolates by their geographic origin. This low correlation suggests that gene transfer may have occurred by parasexual combination in this fungus population. However, in spite of the research efforts for that end, it has not been possible to establish patterns that characterize the profile of B. sorokiniana.
authors.affiliation: Instituto de Ciências Básicas da Saúde (ICBS), Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, RS, CEP 90050-170, Brazil.
authors.forename: Michele B
authors.initials: MB
authors.lastname: Mann
authors.forename: Elisandra
authors.initials: E
authors.lastname: Minotto
authors.forename: Thaisa
authors.initials: T
authors.lastname: Feltrin
authors.forename: Luciana P
authors.initials: LP
authors.lastname: Milagre
authors.forename: Cristina
authors.initials: C
authors.lastname: Spadari
authors.forename: Sueli T
authors.initials: ST
authors.lastname: Van Der Sand
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014908:Triticum / Q000382:microbiology
chemical_list: D004271:DNA, Fungal
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