title: Adhesion and migration of cells responding to microtopography.
abstract: It is known that cells respond strongly to microtopography. However, cellular mechanisms of response are unclear. Here, we study wild-type fibroblasts responding to 25 µm(2) posts and compare their response to that of FAK(-/-) fibroblasts and fibroblasts with PMA treatment to stimulate protein kinase C (PKC) and the small g-protein Rac. FAK knockout cells modulated adhesion number and size in a similar way to cells on topography; that is, they used more, smaller adhesions, but migration was almost completely stalled demonstrating the importance of FAK signaling in contact guidance and adhesion turnover. Little similarity, however, was observed to PKC stimulated cells and cells on the topography. Interestingly, with PKC stimulation the cell nuclei became highly deformable bringing focus on these surfaces to the study of metastasis. Surfaces that aid the study of cellular migration are important in developing understanding of mechanisms of wound healing and repair in aligned tissues such as ligament and tendon.
authors.affiliation: Nanobioengineering Group, Institute for Bioengineering of Catalonia (IBEC), Baldiri Reixac 15-21, Barcelona, 08028, Spain; Biomimetic Systems for Cell Engineering Group, Institute for Bioengineering of Catalonia (IBEC), Baldiri Reixac 15-21, Barcelona, 08028, Spain; Department of Electronics, University of Barcelona, C/Martí i Franquès 1, Barcelona, 08028, Spain.
authors.forename: Maruxa
authors.initials: M
authors.lastname: Estévez
authors.forename: Elena
authors.initials: E
authors.lastname: Martínez
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Yarwood
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
authors.forename: Josep
authors.initials: J
authors.lastname: Samitier
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D048430:Cell Shape; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000172:deficiency / Q000378:metabolism; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D007368:Interferometry; D051379:Mice; D019904:Polymethyl Methacrylate / Q000737:chemistry* / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D016596:Vinculin / Q000378:metabolism
chemical_list: D016596:Vinculin; D019904:Polymethyl Methacrylate; D051416:Focal Adhesion Protein-Tyrosine Kinases; D013755:Tetradecanoylphorbol Acetate
keywords: adhesion; cell migration; cell morphology; focal adhesion kinase; microstructures