title: Isolation and characterization of mouse innate lymphoid cells.
abstract: Innate lymphoid cells (ILCs) are rare populations of cytokine-producing lymphocytes and are divided into three groups, namely ILC1, ILC2, and ILC3, based on the cytokines that they produce. They comprise less than 1% of lymphocytes in mucosal tissues and express no unique cell surface markers. Therefore, they can only be identified by combinations of multiple cell surface markers and further characterized by cytokine production in vitro. Thus, multicolor flow cytometry is the only reliable method to purify and characterize ILCs. Here we describe the methods for cell preparation, flow cytometric analysis, and purification of murine ILC2 and ILC3.
authors.affiliation: Terry Fox Laboratory British Columbia Cancer Agency, Vancouver, British Columbia, Canada.|Genetics Graduate Program, College for Interdisciplinary Studies, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Timotheus Y F
authors.initials: TYF
authors.lastname: Halim
authors.affiliation: Terry Fox Laboratory British Columbia Cancer Agency, Vancouver, British Columbia, Canada.|Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Fumio
authors.initials: F
authors.lastname: Takei
mesh_terms: D000818:Animals; D016207:Cytokines / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D007113:Immunity, Innate; D008214:Lymphocytes / Q000145:classification / Q000166:cytology* / Q000276:immunology*; D051379:Mice
chemical_list: D016207:Cytokines
keywords: Rora; Rorct; flow cytometry; group 2 innate lymphoid cells (ILC2); group 3 innate lymphoid cells (ILC3); innate lymphoid cells (ILC)
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