title: Effect of autochthonous bacteriocin-producing Lactococcus lactis on bacterial population dynamics and growth of halotolerant bacteria in Brazilian charqui.
abstract: Charqui is a fermented, salted and sun-dried meat product, widely consumed in Brazil and exported to several countries. Growth of microorganisms in this product is unlikely due to reduced Aw, but halophilic and halotolerant bacteria may grow and cause spoilage. Charqui is a good source of lactic acid bacteria able to produce antimicrobial bacteriocins. In this study, an autochthonous bacteriocinogenic strain (Lactococcus lactis subsp. lactis 69), isolated from charqui, was added to the meat used for charqui manufacture and evaluated for its capability to prevent the growth of spoilage bacteria during storage up to 45 days. The influence of L. lactis 69 on the bacterial diversity during the manufacturing of the product was also studied, using denaturing gradient gel electrophoresis (DGGE). L. lactis 69 did not affect the counts and diversity of lactic acid bacteria during manufacturing and storage, but influenced negatively the populations of halotolerant microorganisms, reducing the spoilage potential. The majority of tested virulence genes was absent, evidencing the safety and potential technological application of this strain as an additional hurdle to inhibit undesirable microbial growth in this and similar fermented meat products.
authors.affiliation: Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, 580, Professor Lineu Prestes, 13 B, Sao Paulo, SP, 05508-000, Brazil. Electronic address: vbiscola@usp.br.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Biscola
authors.affiliation: Department of Health Sciences, Faculty of Experimental Sciences, University of Jaén, Campus las Lagunillas, s/n, 23071, Jaén, Spain.
authors.forename: Hikmate
authors.initials: H
authors.lastname: Abriouel
authors.affiliation: Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, 580, Professor Lineu Prestes, 13 B, Sao Paulo, SP, 05508-000, Brazil.
authors.forename: Svetoslav Dimitrov
authors.initials: SD
authors.lastname: Todorov
authors.affiliation: Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, 580, Professor Lineu Prestes, 13 B, Sao Paulo, SP, 05508-000, Brazil.
authors.forename: Verena Sant'Anna Cabral
authors.initials: VS
authors.lastname: Capuano
authors.affiliation: Department of Health Sciences, Faculty of Experimental Sciences, University of Jaén, Campus las Lagunillas, s/n, 23071, Jaén, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gálvez
authors.affiliation: Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, 580, Professor Lineu Prestes, 13 B, Sao Paulo, SP, 05508-000, Brazil.
authors.forename: Bernadette Dora Gombossy de Melo
authors.initials: BD
authors.lastname: Franco
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D001430:Bacteriocins / Q000378:metabolism*; D044822:Biodiversity; D001938:Brazil; D005285:Fermentation; D005519:Food Preservation / Q000379:methods*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D008461:Meat Products / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*; D013552:Swine
chemical_list: D001430:Bacteriocins; D012965:Sodium Chloride
keywords: Bacteriocin; Charqui; Halotolerant microorganisms; Lactococcus lactis; Spoilage