title: The effect of elastic biodegradable polyurethane electrospun nanofibers on the differentiation of mesenchymal stem cells.
abstract: Biodegradable polyurethane (PU) was synthesized based on using poly(ɛ-caprolactone) (PCL) as the soft segment. Fibers in different diameters (200-400nm, 600-800nm, and 1.4-1.6μm) were then made by electrospinning PU solution in N,N-dimethylacetamide and 2,2,2-trifluoroethanol. Human bone marrow derived mesenchymal stem cells (hMSCs) in the form of single dispersed cells or aggregates were seeded on the electrospun meshes for evaluation of cell behavior. Differentiation experiments showed that hMSC aggregates on electrospun fibers had greater differentiation capacities than single cells. Besides, nanofibers of 200-400nm diameters significantly promoted the osteogenic and chondrogenic differentiation of hMSCs than fibers of the other diameters. The effect of substrate elasticity was further elucidated by comparing cell behaviors on the nanofibers of PCL-based PU and those of pure PCL. The more elastic PU nanofibers demonstrated more osteogenic and chondrogenic induction potential than PCL electrospun fibers. We suggested that the elastic nanofibers seeded with hMSC aggregates may be advantageous for cartilage and bone tissue engineering.
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Yi-Chia
authors.initials: YC
authors.lastname: Kuo
authors.affiliation: Institute of Clinical Medicine and Institute of Pharmacology, Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; Stem Cell Laboratory, Department of Medical Research & Education and Orthopaedics & Traumatology, Veterans General Hospital, Taipei, Taiwan.
authors.forename: Shih-Chieh
authors.initials: SC
authors.lastname: Hung
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan. Electronic address: shhsu@ntu.edu.tw.
authors.forename: Shan-Hui
authors.initials: SH
authors.lastname: Hsu
mesh_terms: D001483:Base Sequence; D001672:Biocompatible Materials; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D017931:DNA Primers; D004548:Elasticity; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D057139:Nanofibers; D011140:Polyurethanes / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D017931:DNA Primers; D011140:Polyurethanes
keywords: Biodegradable; Electrospinning; Mesenchymal stem cells (MSCs); Nanofibers; Polyurethane (PU)