title: Two euAGAMOUS genes control C-function in Medicago truncatula.
abstract: C-function MADS-box transcription factors belong to the AGAMOUS (AG) lineage and specify both stamen and carpel identity and floral meristem determinacy. In core eudicots, the AG lineage is further divided into two branches, the euAG and PLE lineages. Functional analyses across flowering plants strongly support the idea that duplicated AG lineage genes have different degrees of subfunctionalization of the C-function. The legume Medicago truncatula contains three C-lineage genes in its genome: two euAG genes (MtAGa and MtAGb) and one PLENA-like gene (MtSHP). This species is therefore a good experimental system to study the effects of gene duplication within the AG subfamily. We have studied the respective functions of each euAG genes in M. truncatula employing expression analyses and reverse genetic approaches. Our results show that the M. truncatula euAG- and PLENA-like genes are an example of subfunctionalization as a result of a change in expression pattern. MtAGa and MtAGb are the only genes showing a full C-function activity, concomitant with their ancestral expression profile, early in the floral meristem, and in the third and fourth floral whorls during floral development. In contrast, MtSHP expression appears late during floral development suggesting it does not contribute significantly to the C-function. Furthermore, the redundant MtAGa and MtAGb paralogs have been retained which provides the overall dosage required to specify the C-function in M. truncatula.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV). Ciudad Politécnica de la Innovación, Valencia, Spain.
authors.forename: Joanna
authors.initials: J
authors.lastname: Serwatowska
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV). Ciudad Politécnica de la Innovación, Valencia, Spain.
authors.forename: Edelín
authors.initials: E
authors.lastname: Roque
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV). Ciudad Politécnica de la Innovación, Valencia, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Gómez-Mena
authors.affiliation: Department of Plant Biology, Danish Institute of Agricultural Sciences, Frederiksberg C, Denmark.
authors.forename: Gabriela D
authors.initials: GD
authors.lastname: Constantin
authors.affiliation: Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, United States of America.
authors.forename: Jiangqi
authors.initials: J
authors.lastname: Wen
authors.affiliation: Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, United States of America.
authors.forename: Kirankumar S
authors.initials: KS
authors.lastname: Mysore
authors.affiliation: Department of Plant Biology, Danish Institute of Agricultural Sciences, Frederiksberg C, Denmark.
authors.forename: Ole S
authors.initials: OS
authors.lastname: Lund
authors.affiliation: Department of Plant Biology, Danish Institute of Agricultural Sciences, Frederiksberg C, Denmark.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Johansen
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV). Ciudad Politécnica de la Innovación, Valencia, Spain.
authors.forename: José Pío
authors.initials: JP
authors.lastname: Beltrán
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV). Ciudad Politécnica de la Innovación, Valencia, Spain.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Cañas
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D016000:Cluster Analysis; D035264:Flowers / Q000235:genetics* / Q000254:growth & development; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics*; D017403:In Situ Hybridization; D026161:MADS Domain Proteins / Q000235:genetics*; D046913:Medicago truncatula / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D026161:MADS Domain Proteins
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