title: Role of vascular endothelial growth factor C in classical Hodgkin lymphoma.
abstract: We performed three different studies to obtain additional data on the biological and prognostic role of vascular endothelial growth factor C (VEGFC) in classical Hodgkin lymphoma (cHL): (1) serum VEGFC levels were measured by enzyme-linked immunosorbent assay in 80 patients; (2) immunohistochemical VEGFC expression in tumor tissue was evaluated using a case-control study in 62 patients; (3) gene expression of VEGFC was investigated in vitro in six cHL cell lines, and was compared with expression in inflammatory lymph nodes. Higher serum VEGFC levels were found in patients with cHL than in healthy volunteers (median, 7675 vs. 1358 pg/mL, p < 0.001) and high VEGFC expression in tissue predicted worse progression-free survival at 7 years (41% vs. 91%, p < 0.0001). VEGFC expression in all Hodgkin lymphoma cell lines was higher than the mean expression level in inflammatory lymph nodes. Our data suggest that Hodgkin and Reed-Sternberg cells produce VEGFC, and VEGFC expression could be a novel prognostic factor in cHL.
authors.affiliation: Departamento de Oncohematología, Agencia Sanitaria Costa del Sol, Instituto de Investigación Biomédica de Málaga , Marbella , Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Rueda
authors.forename: David
authors.initials: D
authors.lastname: Olmos
authors.forename: Luis
authors.initials: L
authors.lastname: Vicioso
authors.forename: Cristina
authors.initials: C
authors.lastname: Quero
authors.forename: Elena
authors.initials: E
authors.lastname: Gallego
authors.forename: Bella Isabel
authors.initials: BI
authors.lastname: Pajares-Hachero
authors.forename: Marta
authors.initials: M
authors.lastname: Mendiola
authors.forename: María
authors.initials: M
authors.lastname: Casanova
authors.forename: Martina
authors.initials: M
authors.lastname: Álvarez
authors.forename: Mariano
authors.initials: M
authors.lastname: Provencio
authors.forename: Emilio
authors.initials: E
authors.lastname: Alba
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D015415:Biomarkers; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006689:Hodgkin Disease / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000401:mortality; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D016019:Survival Analysis; D042582:Vascular Endothelial Growth Factor C / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D042582:Vascular Endothelial Growth Factor C
keywords: Classical Hodgkin lymphoma; VEGFC; angiogenesis; lymphangiogenesis