title: Aurora A kinase modulates actin cytoskeleton through phosphorylation of Cofilin: Implication in the mitotic process.
abstract: Aurora A kinase regulates early mitotic events through phosphorylation and activation of a variety of proteins. Specifically, Aur-A is involved in centrosomal separation and formation of mitotic spindles in early prophase. The effect of Aur-A on mitotic spindles is mediated by the modulation of microtubule dynamics and association with microtubule binding proteins. In this study we show that Aur-A exerts its effects on spindle organization through the regulation of the actin cytoskeleton. Aurora A phosphorylates Cofilin at multiple sites including S(3) resulting in the inactivation of its actin depolymerizing function. Aur-A interacts with Cofilin in early mitotic phases and regulates its phosphorylation status. Cofilin phosphorylation follows a dynamic pattern during the progression of prophase to metaphase. Inhibition of Aur-A activity induced a delay in the progression of prophase to metaphase. Aur-A inhibitor also disturbed the pattern of Cofilin phosphorylation, which correlated with the mitotic delay. Our results establish a novel function of Aur-A in the regulation of actin cytoskeleton reorganization, through Cofilin phosphorylation during early mitotic stages.
authors.affiliation: Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Ritchey
authors.affiliation: Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA. Electronic address: ratna.chakrabarti@ucf.edu.
authors.forename: Ratna
authors.initials: R
authors.lastname: Chakrabarti
keywords: Actin cytoskeleton; Aurora A kinase; Cofilin; Mitosis; Phosphorylation
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