title: Genetic deletion in uncoupling protein 3 augments 18F-fluorodeoxyglucose cardiac uptake in the ischemic heart.
abstract: BACKGROUND
  We investigated the effects of uncoupling protein 3 (UCP3) genetic deletion on 18F-fluorodeoxyglucose (FDG) cardiac uptake by positron emission tomography (PET)/computed tomography (CT) dedicated animal system after permanent coronary artery ligation.
  
  
  METHODS
  Cardiac 18F-FDG PET/CT was performed in UCP3 knockout (UCP3-/-) and wild-type (WT) mice one week after induction of myocardial infarction or sham procedure.
  
  
  RESULTS
  In sham-operated mice no difference in left ventricular (LV) volume was detectable between WT and UCP3-/-. After myocardial infarction, LV volume was higher in both WT and UCP3-/- compared to sham animals, with a significant interaction (p < 0.05) between genotype and myocardial infarction. In sham-operated animals no difference in FDG standardized uptake value (SUV) was detectable between WT (1.8 ± 0.6) and UCP3-/- (1.8 ± 0.6). After myocardial infarction SUV was significantly higher in remote areas than in infarcted territories in both UCP3-/- and WT mice (both p < 0.01). Moreover, in remote areas, SUV was significantly higher (p < 0.001) in UCP3-/- as compared to WT, while in the infarcted territory SUV was comparable (p = 0.29). A significant relationship (r = 0.68, p < 0.001) between LV volume and SUV was found.
  
  
  CONCLUSIONS
  In a mice model of permanent coronary occlusion, UCP3 deficiency results in a metabolic shift that favored glycolytic metabolism and increased FDG uptake in remote areas.
authors.forename: Sara
authors.initials: S
authors.lastname: Gargiulo
authors.forename: Maria Piera
authors.initials: MP
authors.lastname: Petretta
authors.forename: Adelaide
authors.initials: A
authors.lastname: Greco
authors.forename: Mariarosaria
authors.initials: M
authors.lastname: Panico
authors.forename: Michele
authors.initials: M
authors.lastname: Larobina
authors.forename: Matteo
authors.initials: M
authors.lastname: Gramanzini
authors.forename: Gabriele G
authors.initials: GG
authors.lastname: Schiattarella
authors.forename: Giovanni
authors.initials: G
authors.lastname: Esposito
authors.forename: Mario
authors.initials: M
authors.lastname: Petretta
authors.forename: Arturo
authors.initials: A
authors.lastname: Brunetti
authors.affiliation: Department of Advanced Biomedical Sciences, University Federico II, Via Pansini 5, 80131 Naples, Italy. cuocolo@unina.it.
authors.forename: Alberto
authors.initials: A
authors.lastname: Cuocolo
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism*; D005838:Genotype; D006019:Glycolysis; D006352:Heart Ventricles / Q000000981:diagnostic imaging* / Q000378:metabolism*; D007473:Ion Channels / Q000172:deficiency* / Q000235:genetics; D008297:Male; D057507:Mice, 129 Strain; D018345:Mice, Knockout; D024101:Mitochondrial Proteins / Q000172:deficiency* / Q000235:genetics; D064847:Multimodal Imaging; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging* / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000378:metabolism*; D014057:Tomography, X-Ray Computed; D000071247:Uncoupling Protein 3
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D019275:Radiopharmaceuticals; C000604393:Ucp3 protein, mouse; D000071247:Uncoupling Protein 3; D019788:Fluorodeoxyglucose F18
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