title: ZCT1 and ZCT2 transcription factors repress the activity of a gene promoter from the methyl erythritol phosphate pathway in Madagascar periwinkle cells.
abstract: In Catharanthus roseus, accumulating data highlighted the existence of a coordinated transcriptional regulation of structural genes that takes place within the secoiridoid biosynthetic branch, including the methyl erythritol phosphate (MEP) pathway and the following steps leading to secologanin. To identify transcription factors acting in these pathways, we performed a yeast one-hybrid screening using as bait a promoter region of the hydroxymethylbutenyl 4-diphosphate synthase (HDS) gene involved in the responsiveness of C. roseus cells to hormonal signals inducing monoterpene indole alkaloid (MIA) production. We identified that ZCT2, one of the three members of the zinc finger Catharanthus protein (ZCT) family, can bind to a HDS promoter region involved in hormonal responsiveness. By trans-activation assays, we demonstrated that ZCT1 and ZCT2 but not ZCT3 repress the HDS promoter activity. Gene expression analyses in C. roseus cells exposed to methyljasmonate revealed a persistence of induction of ZCT2 gene expression suggesting the existence of feed-back regulatory events acting on HDS gene expression in correlation with the MIA production.
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France.
authors.forename: Mouadh
authors.initials: M
authors.lastname: Chebbi
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France.
authors.forename: Olivia
authors.initials: O
authors.lastname: Ginis
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Courdavault
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Glévarec
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Lanoue
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Clastre
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
authors.affiliation: UMR CNRS 7267 EBI, Ecologie et Biologie des Interactions, Equipe, "Sucres & Echanges Végétaux-Environnement", Université de Poitiers, Bâtiment Botanique B31, 3 rue Jacques Fort, TSA 51106, 86073 Poitiers Cedex 9, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Gaillard
authors.affiliation: UMR CNRS 7267 EBI, Ecologie et Biologie des Interactions, Equipe, "Sucres & Echanges Végétaux-Environnement", Université de Poitiers, Bâtiment Botanique B31, 3 rue Jacques Fort, TSA 51106, 86073 Poitiers Cedex 9, France.
authors.forename: Rossitza
authors.initials: R
authors.lastname: Atanassova
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: St-Pierre
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Giglioli-Guivarc'h
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Courtois
authors.affiliation: EA 2106 "Biomolécules et Biotechnologies Végétales"-Université François Rabelais de Tours, 31 avenue Monge, 37200 Tours, France. Electronic address: audrey.oudin@univ-tours.fr.
authors.forename: Audrey
authors.initials: A
authors.lastname: Oudin
mesh_terms: D030008:Catharanthus / Q000201:enzymology* / Q000235:genetics*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D004798:Enzymes; D010940:Plant Proteins; D014157:Transcription Factors; C421242:hydroxymethylbutenyl 4-diphosphate synthase; D004896:Erythritol
keywords: Catharanthus roseus; Hydroxymethylbutenyl 4-diphosphate synthase gene; Methyl erythritol phosphate pathway; Monoterpenoid indole alkaloids; Zinc finger proteins