title: Inhibition of verotoxin (VT) 2 absorption into systemic blood from intestine by repeated administration of bovine immune colostral antibody against VT2 in mice.
abstract: OBJECTIVE
  Whether absorption of verotoxin (VT) 2 from the intestine in mice is inhibited by administration bovine immune colostral antibody against VT2 was investigated.
  
  
  METHODS
  Three-week-old mice were administered VT2 solution at 477.8 ng/mL or 955.6 ng/mL, and bovine immune colostral antibody against VT2 was then administered three times. Whey without antibody against VT2 was administered to control mice. Serum levels of VT2 were measured by fluorescence enzyme immunoassay.
  
  
  RESULTS
  Serum levels of VT2 in mice administered VT2 solution at 477.8 ng/mL and bovine immune colostral antibody against VT2 scarcely changed. By contrast, serum levels of VT2 in control mice increased and peaked 12 hours after administration. Peak values were 15.4 ± 5.04 ng/mL. Furthermore, serum levels of VT2 at 12 hours and 16 hours in control mice were significantly higher than in mice administered bovine colostral antibody against VT2. Serum levels of VT2 in mice administered antibody at 955.6 ng/mL showed no significant differences between repeated administration of bovine immune colostral antibody and controls.
  
  
  CONCLUSIONS
  These results suggest that absorption of VT2 from the intestine was inhibited by repeated administration of bovine immune colostral antibody against VT2 at early stages of Escherichia coli O157:H7 infection, whereas VT2 in the intestine remained at low levels.
authors.affiliation: Graduate School of Environmental and Health Science, Azabu University, 1-17-71 Fuchinobe, Chou-ku Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Seita
authors.initials: S
authors.lastname: Tetsurou
authors.affiliation: Graduate School of Environmental and Health Science, Azabu University, 1-17-71 Fuchinobe, Chou-ku Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuribayashi
authors.affiliation: Laboratory of Microbiology, Faculty of Life and Environmental Science, Azabu University, 1-17-71 Fuchinobe, Chuou-ku Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Fukuyama
authors.affiliation: Department of Pediatrics, Shimane University School of Medicine, 89-1 En-ya-cho, Izumo, Shimane 693-8501, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Yamaguchi
authors.affiliation: Graduate School of Environmental and Health Science, Azabu University, 1-17-71 Fuchinobe, Chou-ku Sagamihara, Kanagawa 252-5201, Japan. Electronic address: yamamoto@azabu-u.ac.jp.
authors.forename: Shizuo
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D003126:Colostrum / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control; D019453:Escherichia coli O157 / Q000276:immunology / Q000472:pathogenicity; D005517:Foodborne Diseases / Q000382:microbiology / Q000517:prevention & control; D041981:Gastrointestinal Tract / Q000382:microbiology / Q000473:pathology; D005849:Gerbillinae; D007071:Immunoglobulin A, Secretory / Q000008:administration & dosage / Q000276:immunology*; D007408:Intestinal Absorption / Q000276:immunology* / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D022641:Shiga Toxin 2 / Q000097:blood* / Q000378:metabolism / Q000633:toxicity*
chemical_list: D007071:Immunoglobulin A, Secretory; D022641:Shiga Toxin 2
keywords: VT2; absorption; bovine colostral antibody; intestine; mice