title: Plasma levels of microRNA-499 provide an early indication of perioperative myocardial infarction in coronary artery bypass graft patients.
abstract: BACKGROUND
  Recent studies indicated that microRNAs (miRNAs, miRs) were important for many biological and pathological processes, and they might be potential biomarkers for cardiovascular diseases. The present study aims to determine the release patterns of miRNAs in cardiac surgery and to analyze the ability of miRs to provide early prediction of perioperative myocardial infarction (PMI) in patients undergoing coronary artery bypass graft (CABG) surgery.
  
  
  RESULTS
  Thirty on-pump CABG patients were recruited in this study; and miR-499, miR-133a and miR-133b, cardiac troponin I (cTnI) were selected for measurement. Serial plasma samples were collected at seven perioperative time points (preoperatively, and 1, 3, 6, 12, 24, and 48 hours after declamping) and were tested for cTnI and miRs levels. Importantly, miR levels peaked as early as 1-3 hours, whereas cTnI levels peaked at 6 hours after declamping. Peak plasma concentrations of miRs correlated significantly with cTnI (miR-499, r = 0.583, P = 0.001; miR-133a, r = 0.514, P = 0.006; miR-133b, r = 0.437, P = 0.05), indicating the degree of myocardial damage. In addition, 30 off-pump CABG patients were recruited; miR-499 and miR-133a levels were tested, which were significantly lower in off-pump group than in on-pump group. A prospective cohort of CABG patients (n = 120) was recruited to study the predictive power of miRs for PMI. The diagnosis of PMI strictly adhered to the principles of universal definition of myocardial infarction. The data analysis revealed that miR-499 had higher sensitivity and specificity than cTnI, and indicated that miR-499 could be an independent risk factor for PMI.
  
  
  CONCLUSIONS
  Our results demonstrate that circulating miR-499 is a novel, early biomarker for identifying perioperative myocardial infarction in cardiac surgery.
authors.affiliation: Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Cardiovascular Diseases, National Center for Cardiovascular Diseases, Peking, China; Department of Cardiac Surgery, Fuwai Hospital and Cardiovascular Institute, Peking, China; Key Laboratory of Cardiac Regenerative Medicine, Ministry of Health, National Center for Cardiovascular Diseases, Peking, China.
authors.forename: Youxiu
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Cardiovascular Diseases, National Center for Cardiovascular Diseases, Peking, China; Department of Cardiac Surgery, Fuwai Hospital and Cardiovascular Institute, Peking, China; Key Laboratory of Cardiac Regenerative Medicine, Ministry of Health, National Center for Cardiovascular Diseases, Peking, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Thoracic Surgery, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Tongzhou, Beijing, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Cao
authors.affiliation: Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Cardiovascular Diseases, National Center for Cardiovascular Diseases, Peking, China; Department of Cardiac Surgery, Fuwai Hospital and Cardiovascular Institute, Peking, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Cardiovascular Diseases, National Center for Cardiovascular Diseases, Peking, China; Department of Cardiac Surgery, Fuwai Hospital and Cardiovascular Institute, Peking, China.
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Cardiovascular Diseases, National Center for Cardiovascular Diseases, Peking, China; Department of Cardiac Surgery, Fuwai Hospital and Cardiovascular Institute, Peking, China; Key Laboratory of Cardiac Regenerative Medicine, Ministry of Health, National Center for Cardiovascular Diseases, Peking, China.
authors.forename: Shengshou
authors.initials: S
authors.lastname: Hu
authors.affiliation: Chinese Academy of Medical Sciences and Peking Union Medical College, State Key Laboratory of Cardiovascular Diseases, National Center for Cardiovascular Diseases, Peking, China; Department of Cardiac Surgery, Fuwai Hospital and Cardiovascular Institute, Peking, China; Key Laboratory of Cardiac Regenerative Medicine, Ministry of Health, National Center for Cardiovascular Diseases, Peking, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zheng
mesh_terms: D015415:Biomarkers / Q000097:blood; D001026:Coronary Artery Bypass / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis* / Q000209:etiology; D009928:Organ Specificity; D059035:Perioperative Period; D011379:Prognosis; D013997:Time Factors; D019210:Troponin I / Q000097:blood
chemical_list: D015415:Biomarkers; C548683:MIRN499 microRNA, human; D035683:MicroRNAs; D019210:Troponin I
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