title: Problem-solving test: Southwestern blotting.
abstract: Terms to be familiar with before you start to solve the test: Southern blotting, Western blotting, restriction endonucleases, agarose gel electrophoresis, nitrocellulose filter, molecular hybridization, polyacrylamide gel electrophoresis, proto-oncogene, c-abl, Src-homology domains, tyrosine protein kinase, nuclear localization signal, cDNA, deletion mutants, expression plasmid, transfection, RNA polymerase II, promoter, Shine-Dalgarno sequence, polyadenylation element, affinity chromatography, Northern blotting, immunoprecipitation, sodium dodecylsulfate, autoradiography, tandem repeats.
authors.affiliation: Department of Medical Biology, Medical School, University of Pécs, H-7624, Pécs, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Szeberényi
mesh_terms: D015139:Blotting, Southern / Q000379:methods; D026501:Blotting, Southwestern / Q000379:methods*; D015153:Blotting, Western / Q000379:methods; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods; D008967:Molecular Biology / Q000193:education / Q000379:methods*; D011340:Problem Solving; D018794:Problem-Based Learning / Q000379:methods; D012319:RNA Polymerase II / Q000378:metabolism; D011795:Surveys and Questionnaires; D009626:Terminology as Topic
chemical_list: D012319:RNA Polymerase II; D004262:DNA Restriction Enzymes