title: Detection of Toxoplasma gondii and Cyclospora cayetanensis in Mytilus galloprovincialis from Izmir Province coast (Turkey) by Real Time PCR/High-Resolution Melting analysis (HRM).
abstract: To evaluate the presence of Toxoplasma gondii and Cyclospora cayetanensis in edible shellfish, a total of 795 specimens of Mytilus galloprovincialis from 8 different sites on the west coast of Turkey (Bays of Izmir and Mersin) were examined. Fifty-three pooled samples were created and subjected to EvaGreen(®) RealTime PCR assay and HRM analysis for protozoans detection and oocyst quantification. Seven out of 8 sites were contaminated with at least one protozoan species. Twenty-one out of 53 (39.6%) samples tested positive for T. gondii, C. cayetanensis or both pathogens: 26.4% samples positive for C. cayetanensis; 9.4% for T. gondii, and 3.8% for both protozoans. The number of oocysts in positive samples ranged from 6 to 30 for T. gondii and from 3 to 48 for C. cayetanensis. This is the first report of T. gondii Type 1, and C. cayetanensis in M. galloprovincialis, the most consumed shellfish species in Turkey.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Dokuz Eylül University, 35340 Izmir, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Aksoy
authors.affiliation: Department of Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71121 Foggia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Marangi
authors.affiliation: Department of Veterinary Sciences, University of Pisa, 56120 Pisa, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Papini
authors.affiliation: Department of Parasitology, Faculty of Medicine, Dokuz Eylül University, 35340 Izmir, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Ozkoc
authors.affiliation: Department of Parasitology, Faculty of Medicine, Dokuz Eylül University, 35340 Izmir, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Bayram Delibas
authors.affiliation: Department of Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71121 Foggia, Italy. Electronic address: annunziata.giangaspero@unifg.it.
authors.forename: A
authors.initials: A
authors.lastname: Giangaspero
mesh_terms: D000818:Animals; D021744:Cyclospora / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000009:adverse effects / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D049878:Mytilus / Q000469:parasitology*; D060888:Real-Time Polymerase Chain Reaction; D012758:Shellfish / Q000469:parasitology*; D014122:Toxoplasma / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D044366:Transition Temperature; D014421:Turkey
chemical_list: D016054:DNA, Protozoan
keywords: Cyclospora cayetanensis; Mytilus galloprovincialis; Public health; RealTime HRM; Toxoplasma gondii Type I