title: Heterologous expression of Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) enhanced chilling tolerance in transgenic eggplant (Solanum melongena L.).
abstract: Our study shows that the expression of AtCBF3 and AtCOR15A improved the chilling tolerance in transgenic eggplant. In an attempt to improve chilling tolerance of eggplant (Solanum melongena L) plants, Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) genes both driven by an Arabidopsis RESPONSIVE TO DESSICATION 29A promoter (AtRD29A) were transferred into the plants of eggplant cultivar Sanyueqie. Two independent homozygous transgenic lines were tested for their cold tolerance. The leaves of the transgenic plants in both lines withered much slower and slighter than the wild-type plants after exposure to cold stress treatment at 2 ± 1 °C. The gene expression of AtCBF3 and AtCOR15A was significantly increased as well as the proline content and the levels of catalase and peroxidase activities, while the relative electrical conductivity and the malondialdehyde content were remarkably decreased in the transgenic plants compared with the wild type at 4 ± 0.5 °C. The results showed that the expression of the exogenous AtCBF3 and AtCOR15A could promote the cold adaptation process to protect eggplant plants from chilling stress.
authors.affiliation: Key Laboratory of Horticulture Science for Southern Mountainous Regions (Chinese Ministry of Education), College of Horticulture and Landscape Architecture, Southwest University, Chongqing, 400715, People's Republic of China, wanfx123@126.com.
authors.forename: Faxiang
authors.initials: F
authors.lastname: Wan
authors.forename: Yu
authors.initials: Y
authors.lastname: Pan
authors.forename: Jinghua
authors.initials: J
authors.lastname: Li
authors.forename: Xiangfu
authors.initials: X
authors.lastname: Chen
authors.forename: Yanglu
authors.initials: Y
authors.lastname: Pan
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Wang
authors.forename: Shibing
authors.initials: S
authors.lastname: Tian
authors.forename: Xingguo
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D008566:Membranes; D010641:Phenotype; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D032323:Solanum melongena / Q000201:enzymology / Q000235:genetics* / Q000502:physiology*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D029681:Arabidopsis Proteins; C116166:COR15 protein, Arabidopsis; C114578:DREB1A protein, Arabidopsis; D014157:Transcription Factors; D011392:Proline
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