title: The normative value of inflammatory cells in the nasal perfusate of Chinese adults: a pilot study.
abstract: OBJECTIVE
  To establish stable, well-accepted nasal perfusion and a normative value of classifying cells in the nasal perfusate of Chinese adults.
  
  
  METHODS
  A total of 500 healthy adults were divided into two groups of 250 people per group (group A, 16-30 years old and group B, 31-60 years old; male-to-female ratio, 1:1). All volunteers were non-smokers; they were irrigated with saline, and multiple inflammatory cells in the perfusate were analyzed.
  
  
  RESULTS
  Irrigation was successfully performed in 479 cases, a success rate of 95.80%. The types of inflammatory cells showed a skewed distribution. The median number and interquartile range (IQR) of eosinophils were 0 and 0.2, respectively. These values were 0.4 and 2.2, respectively, for neutrophils and 0 and 0, respectively, for both lymphocytes and macrophages. There was no significant difference between males and females (P>0.05). There was a significant difference in the numbers of neutrophils and lymphocytes in the different age groups (P=0.000), but there was no significant difference in the numbers of eosinophils and macrophages (P>0.05). The 95% unilateral upper limited values (UULVs) of eosinophils and neutrophils in the nasal perfusates were 2.99 and 14.94, respectively, for group A and 1.41 and 17.08 for group B. As a result, the total 95% UULVs of eosinophils and neutrophils in the nasal perfusate were 2.00 and 16.80.
  
  
  CONCLUSIONS
  We established stable, well-accepted nasal perfusions and normal values for classifying the cells in the nasal perfusate of Chinese adults; the normative values of the inflammatory cells in nasal perfusate are 2.00 for the 95% UULV of eosinophils and 16.80 for neutrophils. Age might be one of the factors affecting the cells in rhinitis.
authors.affiliation: 1 Department of ENT & Head & Neck Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China ; 2 The First Affiliated Hospital of Guangzhou Medical School, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 1 Department of ENT & Head & Neck Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China ; 2 The First Affiliated Hospital of Guangzhou Medical School, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Wang
authors.affiliation: 1 Department of ENT & Head & Neck Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China ; 2 The First Affiliated Hospital of Guangzhou Medical School, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Xie
authors.affiliation: 1 Department of ENT & Head & Neck Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China ; 2 The First Affiliated Hospital of Guangzhou Medical School, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: 1 Department of ENT & Head & Neck Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China ; 2 The First Affiliated Hospital of Guangzhou Medical School, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China.
authors.forename: Derong
authors.initials: D
authors.lastname: Li
authors.affiliation: 1 Department of ENT & Head & Neck Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China ; 2 The First Affiliated Hospital of Guangzhou Medical School, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: 1 Department of ENT & Head & Neck Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China ; 2 The First Affiliated Hospital of Guangzhou Medical School, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China.
authors.forename: Xinju
authors.initials: X
authors.lastname: Pang
authors.affiliation: 1 Department of ENT & Head & Neck Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China ; 2 The First Affiliated Hospital of Guangzhou Medical School, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Yu
authors.affiliation: 1 Department of ENT & Head & Neck Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China ; 2 The First Affiliated Hospital of Guangzhou Medical School, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China.
authors.forename: Nanshan
authors.initials: N
authors.lastname: Zhong
keywords: Nasal perfusate; healthy Chinese adults; methodology; reference value
references.citation: Chest. 2008 Feb;133(2):412-9
references.pmid: 18252914
references.citation: Inhal Toxicol. 1999 Aug;11(8):657-74
references.pmid: 10477441
references.citation: Biomed Pharmacother. 2003 Oct;57(8):366-71
references.pmid: 14568231
references.citation: Ann Allergy Asthma Immunol. 2012 Mar;108(3):141-4
references.pmid: 22374194
references.citation: J Occup Environ Hyg. 2004 Feb;1(2):82-7
references.pmid: 15204882
references.citation: Pediatr Allergy Immunol. 2007 Nov;18 Suppl 18:50-2
references.pmid: 17767609
references.citation: Cell Mol Life Sci. 2007 May;64(10):1269-89
references.pmid: 17364144
references.citation: Int J Immunopathol Pharmacol. 2009 Oct-Dec;22(4):1077-87
references.pmid: 20074472
references.citation: Eur Arch Otorhinolaryngol. 2004 May;261(5):242-6
references.pmid: 14551791
references.citation: J Leukoc Biol. 2004 Aug;76(2):291-9
references.pmid: 15039467
references.citation: Int Arch Allergy Immunol. 2010;151(2):137-41
references.pmid: 19752567
references.citation: J Allergy Clin Immunol. 2010 Oct;126(4):690-9; quiz 700-1
references.pmid: 20920759
references.citation: Acta Otolaryngol. 2004 Jun;124(5):616-20
references.pmid: 15267182
references.citation: Rhinology. 2000 Mar;38(1):23-32
references.pmid: 10780044
references.citation: Kulak Burun Bogaz Ihtis Derg. 2006;16(2):60-3
references.pmid: 16763418
references.citation: J Pediatr. 1957 Nov;51(5):502-9
references.pmid: 13476334
references.citation: Allergy Asthma Proc. 1997 Nov-Dec;18(6):363-6
references.pmid: 9429670
references.citation: Clin Exp Allergy. 2008 Feb;38(2):260-75
references.pmid: 18167126
references.citation: J Allergy Clin Immunol. 2001 Jul;108(1 Suppl):S16-25
references.pmid: 11449202
references.citation: Immun Ageing. 2010 Jul 05;7:8
references.pmid: 20602786
references.citation: Allergy. 2008 Apr;63 Suppl 86:8-160
references.pmid: 18331513
references.citation: Inflamm Allergy Drug Targets. 2011 Dec;10(6):472-6
references.pmid: 21999180
references.citation: Eur J Clin Microbiol Infect Dis. 2011 Sep;30(9):1105-9
references.pmid: 21359623
references.citation: Am Rev Respir Dis. 1980 Nov;122(5):709-19
references.pmid: 7447155
references.citation: Exp Gerontol. 2008 Aug;43(8):718-28
references.pmid: 18586079
references.citation: Environ Health Perspect. 2003 Jun;111(7):925-9
references.pmid: 12782493
references.citation: Int Arch Allergy Immunol. 2005 Mar;136(3):266-72
references.pmid: 15722636
references.citation: Am J Rhinol. 2008 May-Jun;22(3):271-5
references.pmid: 18588759
references.citation: Am J Otolaryngol. 2009 Sep-Oct;30(5):312-7
references.pmid: 19720248
references.citation: Pneumonol Alergol Pol. 2010;78(4):263-70
references.pmid: 20665446
references.citation: Allergy. 1998 Mar;53(3):326-8
references.pmid: 9542618
references.citation: Res Rep Health Eff Inst. 2003 Dec;(112):1-30; discussion 51-67
references.pmid: 14738208
references.citation: Ann Allergy Asthma Immunol. 2012 Jul;109(1):20-8
references.pmid: 22727153