title: Organization of monosynaptic inputs to the serotonin and dopamine neuromodulatory systems.
abstract: Serotonin and dopamine are major neuromodulators. Here, we used a modified rabies virus to identify monosynaptic inputs to serotonin neurons in the dorsal and median raphe (DR and MR). We found that inputs to DR and MR serotonin neurons are spatially shifted in the forebrain, and MR serotonin neurons receive inputs from more medial structures. Then, we compared these data with inputs to dopamine neurons in the ventral tegmental area (VTA) and substantia nigra pars compacta (SNc). We found that DR serotonin neurons receive inputs from a remarkably similar set of areas as VTA dopamine neurons apart from the striatum, which preferentially targets dopamine neurons. Our results suggest three major input streams: a medial stream regulates MR serotonin neurons, an intermediate stream regulates DR serotonin and VTA dopamine neurons, and a lateral stream regulates SNc dopamine neurons. These results provide fundamental organizational principles of afferent control for serotonin and dopamine.
authors.affiliation: Center for Brain Science, Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
authors.forename: Sachie K
authors.initials: SK
authors.lastname: Ogawa
authors.affiliation: Center for Brain Science, Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA. Electronic address: jeremiah.cohen@jhmi.edu.
authors.forename: Jeremiah Y
authors.initials: JY
authors.lastname: Cohen
authors.affiliation: Center for Brain Science, Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
authors.forename: Dabin
authors.initials: D
authors.lastname: Hwang
authors.affiliation: Center for Brain Science, Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
authors.forename: Naoshige
authors.initials: N
authors.lastname: Uchida
authors.affiliation: Center for Brain Science, Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA. Electronic address: mitsuko@mcb.harvard.edu.
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Watabe-Uchida
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology; D004298:Dopamine / Q000502:physiology*; D059290:Dopaminergic Neurons / Q000502:physiology; D005260:Female; D019262:Habenula / Q000166:cytology; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009434:Neural Pathways / Q000166:cytology; D018377:Neurotransmitter Agents / Q000502:physiology*; D059326:Serotonergic Neurons / Q000502:physiology; D012701:Serotonin / Q000502:physiology*; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission
chemical_list: D018377:Neurotransmitter Agents; D012701:Serotonin; D004298:Dopamine
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