title: Evaluation of a Pyrenophora teres f. teres mapping population reveals multiple independent interactions with a region of barley chromosome 6H.
abstract: The necrotrophic fungal pathogen Pyrenophora teres f. teres causes the foliar disease net form net blotch (NFNB) on barley. To investigate the genetics of virulence in the barley- P. teres f. teres pathosystem, we evaluated 118 progeny derived from a cross between the California isolates 15A and 6A on the barley lines Rika and Kombar, chosen based on their differential reactions to isolates 15A and 6A for NFNB disease. Genetic maps generated with SNP, SSR, and AFLP markers were scanned for quantitative trait loci (QTL) associated with virulence in P. teres f. teres. Loci underlying two major QTL, VR1 and VR2, were associated with virulence on Rika barley, accounting for 35% and 20% of the disease reaction type variation, respectively. Two different loci, VK1 and VK2, were shown to underlie two major QTL associated with virulence on Kombar barley accounting for 26% and 19% of the disease reaction type variation, respectively. Progeny isolates harboring VK1, VK2, or VR2 alone were inoculated onto a Rika×Kombar recombinant inbred line mapping population and the susceptibility induced by each pathogen genotype corresponded to the same region on barley chromosome 6H as that identified for the parental isolates 15A and 6A. The data presented here indicate that the P. teres f. teres - barley interaction can at least partially be explained by pathogen-produced necrotrophic effectors (NEs) that interact with dominant barley susceptibility genes resulting in NE triggered susceptibility (NETS).
authors.affiliation: Dept. of Plant Pathology, North Dakota State Univ., Fargo, ND 58102, USA.
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Shjerve
authors.affiliation: USDA-ARS, Cereal Crops Research Unit, Northern Crop Science Lab, Fargo, ND 58102, USA.
authors.forename: Justin D
authors.initials: JD
authors.lastname: Faris
authors.affiliation: Dept. of Plant Pathology, North Dakota State Univ., Fargo, ND 58102, USA.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Brueggeman
authors.affiliation: Dept. of Computer Science, North Dakota State Univ., Fargo, ND 58108, USA.
authors.forename: Changhui
authors.initials: C
authors.lastname: Yan
authors.affiliation: Dept. of Computer Science, North Dakota State Univ., Fargo, ND 58108, USA.
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Dept. of Plant Pathology, North Dakota State Univ., Fargo, ND 58102, USA.
authors.forename: Vaidehi
authors.initials: V
authors.lastname: Koladia
authors.affiliation: Dept. of Plant Pathology, North Dakota State Univ., Fargo, ND 58102, USA; USDA-ARS, Cereal Crops Research Unit, Northern Crop Science Lab, Fargo, ND 58102, USA. Electronic address: timothy.friesen@ars.usda.gov.
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Friesen
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001203:Ascomycota / Q000235:genetics* / Q000472:pathogenicity; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D056426:Genetic Loci; D005819:Genetic Markers; D001467:Hordeum / Q000235:genetics / Q000382:microbiology*; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D011995:Recombination, Genetic; D014774:Virulence
chemical_list: D005819:Genetic Markers
keywords: Genotype by sequencing; Necrotrophic effector triggered susceptibility; Net form net blotch; Pyrenophora teres; Single nucleotide polymorphism