title: Patchwork structure-function analysis of the Sendai virus matrix protein.
abstract: Paramyxoviruses contain a bi-lipidic envelope decorated by two transmembrane glycoproteins and carpeted on the inner surface with a layer of matrix proteins (M), thought to bridge the glycoproteins with the viral nucleocapsids. To characterize M structure-function features, a set of M domains were mutated or deleted. The genes encoding these modified M were incorporated into recombinant Sendai viruses and expressed as supplemental proteins. Using a method of integrated suppression complementation system (ISCS), the functions of these M mutants were analyzed in the context of the infection. Cellular membrane association, localization at the cell periphery, nucleocapsid binding, cellular protein interactions and promotion of viral particle formation were characterized in relation with the mutations. At the end, lack of nucleocapsid binding go together with lack of cell surface localization and both features definitely correlate with loss of M global function estimated by viral particle production.
authors.affiliation: Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland. Electronic address: genevieve.mottet@unige.ch.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Mottet-Osman
authors.affiliation: Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland. Electronic address: vincent.miazza@nestle.com.
authors.forename: Vincent
authors.initials: V
authors.lastname: Miazza
authors.affiliation: Viral Genomics and Vaccination Unit, Institut Pasteur, CNRS UMR3569, Paris, France. Electronic address: Vidalain@pasteur.fr.
authors.forename: Pierre-Olivier
authors.initials: PO
authors.lastname: Vidalain
authors.affiliation: Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland. Electronic address: laurent.roux@unige.ch.
authors.forename: Laurent
authors.initials: L
authors.lastname: Roux
mesh_terms: D002462:Cell Membrane / Q000821:virology; D006801:Humans; D010253:Respirovirus Infections / Q000821:virology*; D029082:Sendai virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019065:Virus Assembly
chemical_list: C097020:M protein, Sendai virus; D014763:Viral Matrix Proteins
keywords: Integrated suppression complementation system; Matrix protein; Paramyxovirus assembly; Sendai virus; Structure-function