title: Characterization of protein expression ofPlatanuspollen following exposure to gaseous pollutants and vehicle exhaust particles.
abstract: Being major ornamental street trees, species ofPlatanusare widely planted in the Shanghai urban area. A great deal of allergenicPlatanuspollen is released from the trees and suspended in the atmosphere during its flowering season, ultimately causing allergic respiratory diseases. Few papers have focused on the distribution of this type of pollen and its expression of allergenic proteins. In order to investigate any differences in protein expression inPlatanuspollen following exposure to gaseous and particulate pollutants, a special apparatus was designed. Exposure condition (such as temperature, humidity, and exposure time) ofPlatanuspollen and gaseous pollutants can be simulated using of this apparatus. FreshPlatanus orientalispollen, pollutant gases (NO2, SO2, NH3), and typical urban ambient particles (vehicle exhaust particles, VEPs) were mixed in this device to examine possible changes that might occur in ambient airborne urban pollen following exposure to such pollutants. Our results showed that the freshP. orientalispollen became swollen, and new kinds of particles could be found on the surface of the pollen grains after exposure to the pollutants. The results of SDS-PAGE showed that five protein bands with molecular weights of 17-19, 34, 61, 82, and 144 kDa, respectively, were detected and gray scale of these brands increased after the pollen exposure to gaseous pollutants. The two-dimensional gel electrophoresis analysis demonstrated that aPlatanuspollen allergenic protein (Pla a1, with a molecular weight of 18 kDa) increased in abundance following exposure to pollutant gases and VEPs, implying that air pollutants may exacerbate the allergenicity of pollen.
authors.affiliation: School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444 China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Lu
authors.affiliation: School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444 China.
authors.forename: Jingjin
authors.initials: J
authors.lastname: Ren
authors.affiliation: School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444 China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Hao
authors.affiliation: School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444 China.
authors.forename: Dingyu
authors.initials: D
authors.lastname: Liu
authors.affiliation: School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444 China.
authors.forename: Rongci
authors.initials: R
authors.lastname: Zhang
authors.affiliation: School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444 China.
authors.forename: Minghong
authors.initials: M
authors.lastname: Wu
authors.affiliation: School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444 China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yi
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800 China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: Center for Environmental Science in Saitama, Saitama, 374-0115 Japan.
authors.forename: Yonemochi
authors.initials: Y
authors.lastname: Shinich
authors.affiliation: School of Science and Engineering, Saitama University, Saitama, 338-8570 Japan.
authors.forename: Qingyue
authors.initials: Q
authors.lastname: Wang
keywords: Air pollution; Platanus pollen; Protein expression
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