title: Reduction and characterization of bioaerosols in a wastewater treatment station via ventilation.
abstract: Bioaerosols from wastewater treatment processes are a significant subgroup of atmospheric aerosols. In the present study, airborne microorganisms generated from a wastewater treatment station (WWTS) that uses an oxidation ditch process were diminished by ventilation. Conventional sampling and detection methods combined with cloning/sequencing techniques were applied to determine the groups, concentrations, size distributions, and species diversity of airborne microorganisms before and after ventilation. There were 3021 ± 537 CFU/m³ of airborne bacteria and 926 ± 132 CFU/m³ of airborne fungi present in the WWTS bioaerosol. Results showed that the ventilation reduced airborne microorganisms significantly compared to the air in the WWTS. Over 60% of airborne bacteria and airborne fungi could be reduced after 4 hr of air exchange. The highest removal (92.1% for airborne bacteria and 89.1% for fungi) was achieved for 0.65-1.1 μm sized particles. The bioaerosol particles over 4.7 μm were also reduced effectively. Large particles tended to be lost by gravitational settling and small particles were generally carried away, which led to the relatively easy reduction of bioaerosol particles 0.65-1.1 μm and over 4.7 μm in size. An obvious variation occurred in the structure of the bacterial communities when ventilation was applied to control the airborne microorganisms in enclosed spaces.
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: gxs0122@rcees.ac.cn.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Regional Water Quality Control Board, Los Angeles, CA 90013, USA.
authors.forename: Pianpian
authors.initials: P
authors.lastname: Wu
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Ding
authors.affiliation: Beijing University of Civil Engineering and Architecture, Beijing 100044, China.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: leel@rcees.ac.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
mesh_terms: D000336:Aerosols / Q000737:chemistry; D000391:Air Microbiology; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D058974:Phylogeography; D013997:Time Factors; D014691:Ventilation; D064091:Waste Disposal Facilities; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D000336:Aerosols; D004269:DNA, Bacterial
keywords: Bioaerosols; Clone library; Genetic; Vertical oxidation ditch; Wastewater treatment station