title: Solid lipid nanoparticles loaded with fluorescent-labelled Cyclosporine A: anti-inflammatory activity in vitro.
abstract: FMOC-isocyclosporine A, a fluorescent labeled cyclosporine A, was encapsulated in solid lipid nanoparticles (SLN) prepared by the coacervation technique, and its anti-inflammatory activity was evaluated. The anti-inflammatory activity of the fluorescent labelled molecule, measured as inhibition of TNF-α secretion, is similar to the native one. SLN were compared to commercial formulations, through measurement of cytokine release and drug uptake in rat peripheral blood mononuclear cells. Drug-loaded SLN inhibit TNF-α secretion in a lower extent than commercial formulations, probably due to a lower uptake by the cells, but the increase of IL-10 secretion caused by the lipid matrix itself makes this formulation interesting for its anti-inflammatory activity.
authors.forename: Marina
authors.initials: M
authors.lastname: Gallarate
authors.forename: Loredana
authors.initials: L
authors.lastname: Serpe
authors.forename: Federica
authors.initials: F
authors.lastname: Foglietta
authors.forename: Gian Paolo
authors.initials: GP
authors.lastname: Zara
authors.forename: Susanna
authors.initials: S
authors.lastname: Giordano
authors.forename: Elena
authors.initials: E
authors.lastname: Peira
authors.forename: Daniela
authors.initials: D
authors.lastname: Chirio
authors.affiliation: Universita degli Studi di Torino, Dipartimento di Scienza e Tecnologia del Farmaco, Via Pietro Giuria 9, Torino, Italy. luigi.battaglia@unito.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Battaglia
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology; D002478:Cells, Cultured; D016572:Cyclosporine / Q000737:chemistry* / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology; D016753:Interleukin-10 / Q000032:analysis / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D005456:Fluorescent Dyes; D008055:Lipids; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D016572:Cyclosporine