title: Preparation and characterization of antioxidant nanoparticles composed of chitosan and fucoidan for antibiotics delivery.
abstract: In this study, we developed novel chitosan/fucoidan nanoparticles (CS/F NPs) using a simple polyelectrolyte self-assembly method and evaluated their potential to be antioxidant carriers. As the CS/F weight ratio was 5/1, the CS/F NPs were spherical and exhibited diameters of approximately 230-250 nm, as demonstrated by TEM. These CS/F NPs maintained compactness and stability for 25 day in phosphate-buffered saline (pH 6.0-7.4). The CS/F NPs exhibited highly potent antioxidant effects by scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing the concentration of intracellular reactive oxygen species (ROS) and superoxide anion (O2-) in stimulated macrophages. The DPPH scavenging effect of CS/F NPs primarily derives from fucoidan. Furthermore, these CS/F NPs activated no host immune cells into inflammation-mediated cytotoxic conditions induced by IL-6 production and NO generation. The MTT cell viability assay revealed an absence of toxicity in A549 cells after exposure to the formulations containing 0.375 mg NPs/mL to 3 mg NPs/mL. Gentamicin (GM), an antibiotic, was used as a model drug for an in vitro releasing test. The CS/F NPs controlled the release of GM for up to 72 h, with 99% of release. The antioxidant CS/F NPs prepared in this study could thus be effective in delivering antibiotics to the lungs, particularly for airway inflammatory diseases.
authors.affiliation: Department of Food Science, College of Life Science, National Taiwan Ocean University, 2 Pei Ning Road, Keelung 20224, Taiwan. ychuang@mail.ntou.edu.tw.
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Department of Food Science, College of Life Science, National Taiwan Ocean University, 2 Pei Ning Road, Keelung 20224, Taiwan. d91548006@ntu.edu.tw.
authors.forename: Rou-Ying
authors.initials: RY
authors.lastname: Li
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry*; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry; D010316:Particle Size; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D004337:Drug Carriers; D015850:Interleukin-6; D010851:Picrates; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D013481:Superoxides; D009569:Nitric Oxide; D048271:Chitosan; C007789:fucoidan; C004931:1,1-diphenyl-2-picrylhydrazyl
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