title: An optoelectrokinetic technique for programmable particle manipulation and bead-based biosignal enhancement.
abstract: Technologies that can enable concentration of low-abundance biomarkers are essential for early diagnosis of diseases. In this study, an optoelectrokinetic technique, termed Rapid Electrokinetic Patterning (REP), was used to enable dynamic particle manipulation in bead-based bioassays. Various manipulation capabilities, such as micro/nanoparticle aggregation, translation, sorting and patterning, were developed. The technique allows for versatile multi-parameter (voltage, light intensity and frequency) based modulation and dynamically addressable manipulation with simple device fabrication. Signal enhancement of a bead-based bioassay was demonstrated using dilute biotin-fluorescein isothiocyanate (FITC) solutions mixed with streptavidin-conjugated particles and rapidly concentrated with the technique. As compared with a conventional ELISA reader, the REP-enabled detection achieved a minimal readout of 3.87 nM, which was a 100-fold improvement in sensitivity. The multi-functional platform provides an effective measure to enhance detection levels in more bead-based bioassays.
authors.affiliation: Department of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan. oswaldchuang@mail.ncku.edu.tw.
authors.forename: Kuan-Chih
authors.initials: KC
authors.lastname: Wang
authors.forename: Aloke
authors.initials: A
authors.lastname: Kumar
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Williams
authors.forename: Nicolas G
authors.initials: NG
authors.lastname: Green
authors.forename: Kyung Chun
authors.initials: KC
authors.lastname: Kim
authors.forename: Han-Sheng
authors.initials: HS
authors.lastname: Chuang
mesh_terms: D001710:Biotin / Q000737:chemistry*; D055664:Electrochemical Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D007700:Kinetics; D010316:Particle Size; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D001710:Biotin; D019809:Streptavidin; D016650:Fluorescein-5-isothiocyanate