title: Application of two newly identified and characterized feruloyl esterases from Streptomyces sp. in the enzymatic production of ferulic acid from agricultural biomass.
abstract: Ferulic acid (FA), a component of hemicellulose in plant cell walls, is a phenolic acid with several potential applications based on its antioxidant properties. Recent studies have shown that feruloyl esterase (FAE) is a key bacterial enzyme involved in FA production from agricultural biomass. In this study, we screened a library of 43 esterases from Streptomyces species and identified two enzymes, R18 and R43, that have FAE activity toward ethyl ferulate. In addition, we characterized their enzyme properties in detail. R18 and R43 showed esterase activity toward other hydroxycinnamic acid esters as well, such as methyl p-coumarate, methyl caffeate, and methyl sinapinate. The amino acid sequences of R18 and R43 were neither similar to each other, nor to other FAEs. We found that R18 and R43 individually showed the ability to produce FA from corn bran; however, combination with other Streptomyces enzymes, namely xylanase and α-l-arabinofuranosidase, increased FA production from biomass such as corn bran, defatted rice bran, and wheat bran. These results suggest that R18 and R43 are effective FAEs for the enzymatic production of FA from biomass.
authors.affiliation: Research Institute for Biological Sciences (RIBS), Okayama Prefectural Technology Center for Agriculture, Forestry, and Fisheries, Kaga-gun, Okayama, Japan.
authors.forename: Misugi
authors.initials: M
authors.lastname: Uraji
authors.affiliation: Department of Agricultural, Biological, and Environmental Sciences, Faculty of Agriculture, Tottori University, Tottori, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Arima
authors.affiliation: Satake Corporation, Higashi-Hiroshima-shi, Hiroshima, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Inoue
authors.affiliation: Enzyme Division, Bio Chemicals Department, Nagase ChemteX Corporation, Fukuchiyama, Kyoto, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Harazono
authors.affiliation: Research Institute for Biological Sciences (RIBS), Okayama Prefectural Technology Center for Agriculture, Forestry, and Fisheries, Kaga-gun, Okayama, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hatanaka
mesh_terms: D000383:Agriculture; D001673:Biodegradation, Environmental; D018533:Biomass; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D004789:Enzyme Activation; D005285:Fermentation; D006868:Hydrolysis; D007700:Kinetics; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D003373:Coumaric Acids; D008670:Metals; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase
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