title: Size-controlled biodegradable nanoparticles: preparation and size-dependent cellular uptake and tumor cell growth inhibition.
abstract: Biodegradable nanoparticles with diameters below 1000nm are of great interest in the contexts of targeted delivery and imaging. In this study, we prepared PLGA nanoparticles with well-defined sizes of ∼70nm (NP70), ∼100nm (NP100), ∼200nm (NP200), ∼400nm (NP400), ∼600nm (NP600) and ∼1000nm (NP1000) using facile fabrication methods based on a nanoprecipitation and solvent evaporation techniques. The nanoparticles showed a narrow size distribution with high yield. Then the size-controlled biodegradable nanoparticles were used to investigate how particle size at nanoscale affects interactions with tumor cells and macrophages. Interestingly, an opposite size-dependent interaction was observed in the two cells. As particle size gets smaller, cellular uptake increased in tumor cells and decreased in macrophages. We also found that paclitaxel (PTX)-loaded nanoparticles showed a size-dependent inhibition of tumor cell growth and the size-dependency was influenced by cellular uptake and PTX release. The size-controlled biodegradable nanoparticles described in this study would provide a useful means to further elucidate roles of particle size on various biomedical applications.
authors.affiliation: College of Pharmacy, Pusan National University, Busan, South Korea; College of Pharmacy, Chosun University, Gwangju, South Korea.
authors.forename: Jin-Seok
authors.initials: JS
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Pusan National University, Busan, South Korea.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Pharmacy, Pusan National University, Busan, South Korea.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Naeem
authors.affiliation: College of Pharmacy, Pusan National University, Busan, South Korea.
authors.forename: Jinki
authors.initials: J
authors.lastname: Noh
authors.affiliation: College of Pharmacy, Pusan National University, Busan, South Korea.
authors.forename: Nurhasni
authors.initials: N
authors.lastname: Hasan
authors.affiliation: College of Pharmacy, Chosun University, Gwangju, South Korea.
authors.forename: Hoo-Kyun
authors.initials: HK
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Pusan National University, Busan, South Korea. Electronic address: jinwook@pusan.ac.kr.
authors.forename: Jin-Wook
authors.initials: JW
authors.lastname: Yoo
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology; D001672:Biocompatible Materials; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D009369:Neoplasms / Q000473:pathology*; D017239:Paclitaxel / Q000008:administration & dosage / Q000494:pharmacology; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017239:Paclitaxel
keywords: Biodegradable nanoparticles; Cellular uptake; Particle size; Tumor cell growth inhibiton