title: Cryptococcus laurentii extracellular biopolymer production for application in wound management.
abstract: Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied. Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved. EPS synthesis and cell growth had different optima. For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %). As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased. Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS. Aeration intensity and time of cultivation had little effect on EPS composition. Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352. The row EPS was composed of acidic glucuronoxylomannan for more than 85 %. In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats. The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
authors.affiliation: Contipro Group s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic. Dzianis.Smirnou@contipro.com.
authors.forename: Dzianis
authors.initials: D
authors.lastname: Smirnou
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic.
authors.forename: Dana
authors.initials: D
authors.lastname: Hrubošová
authors.affiliation: Contipro Pharma a.s., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic.
authors.forename: Jaromír
authors.initials: J
authors.lastname: Kulhánek
authors.affiliation: Contipro Pharma a.s., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic.
authors.forename: Karol
authors.initials: K
authors.lastname: Švík
authors.affiliation: Contipro Group s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Bobková
authors.affiliation: Contipro Pharma a.s., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Moravcová
authors.affiliation: Contipro Group s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Krčmář
authors.affiliation: Contipro Group s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Franke
authors.affiliation: Contipro Group s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Velebný
mesh_terms: D000818:Animals; D003454:Cryptococcus / Q000254:growth & development / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000096:biosynthesis / Q000494:pharmacology; D051381:Rats; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy*
chemical_list: D062610:Fungal Polysaccharides