title: Voice prosthetic biofilm formation and Candida morphogenic conversions in absence and presence of different bacterial strains and species on silicone-rubber.
abstract: Morphogenic conversion of Candida from a yeast to hyphal morphology plays a pivotal role in the pathogenicity of Candida species. Both Candida albicans and Candida tropicalis, in combination with a variety of different bacterial strains and species, appear in biofilms on silicone-rubber voice prostheses used in laryngectomized patients. Here we study biofilm formation on silicone-rubber by C. albicans or C. tropicalis in combination with different commensal bacterial strains and lactobacillus strains. In addition, hyphal formation in C. albicans and C. tropicalis, as stimulated by Rothia dentocariosa and lactobacilli was evaluated, as clinical studies outlined that these bacterial strains have opposite results on the clinical life-time of silicone-rubber voice prostheses. Biofilms were grown during eight days in a silicone-rubber tube, while passing the biofilms through episodes of nutritional feast and famine. Biofilms consisting of combinations of C. albicans and a bacterial strain comprised significantly less viable organisms than combinations comprising C. tropicalis. High percentages of Candida were found in biofilms grown in combination with lactobacilli. Interestingly, L. casei, with demonstrated favorable effects on the clinical life-time of voice prostheses, reduced the percentage hyphal formation in Candida biofilms as compared with Candida biofilms grown in absence of bacteria or grown in combination with R. dentocariosa, a bacterial strain whose presence is associated with short clinical life-times of voice prostheses.
authors.affiliation: University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, Groningen, the Netherlands.
authors.forename: Henny C
authors.initials: HC
authors.lastname: van der Mei
authors.affiliation: University of Groningen and University Medical Center Groningen, Department of Otorhinolaryngology and Head and Neck Surgery, Groningen, the Netherlands.
authors.forename: Kevin J D A
authors.initials: KJ
authors.lastname: Buijssen
authors.affiliation: University of Groningen and University Medical Center Groningen, Department of Otorhinolaryngology and Head and Neck Surgery, Groningen, the Netherlands.
authors.forename: Bernard F A M
authors.initials: BF
authors.lastname: van der Laan
authors.affiliation: University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, Groningen, the Netherlands.
authors.forename: Ekatarina
authors.initials: E
authors.lastname: Ovchinnikova
authors.affiliation: University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, Groningen, the Netherlands.
authors.forename: Gésinda I
authors.initials: GI
authors.lastname: Geertsema-Doornbusch
authors.affiliation: University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, Groningen, the Netherlands.
authors.forename: Jelly
authors.initials: J
authors.lastname: Atema-Smit
authors.affiliation: University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, Groningen, the Netherlands.
authors.forename: Betsy
authors.initials: B
authors.lastname: van de Belt-Gritter
authors.affiliation: University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, Groningen, the Netherlands.
authors.forename: Henk J
authors.initials: HJ
authors.lastname: Busscher
mesh_terms: D018407:Bacterial Physiological Phenomena; D018441:Biofilms / Q000254:growth & development*; D002176:Candida albicans / Q000502:physiology*; D041022:Candida tropicalis / Q000502:physiology*; D006801:Humans; D025301:Hyphae / Q000502:physiology; D007831:Larynx, Artificial / Q000382:microbiology*; D056265:Microbial Interactions; D012826:Silicone Elastomers; D013045:Species Specificity
chemical_list: D012826:Silicone Elastomers
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