title: Enhancement of phototoxicity against human pancreatic cancer cells with photosensitizer-encapsulated amphiphilic sodium alginate derivative nanoparticles.
abstract: Photosensitizer-encapsulated amphiphilic sodium alginate derivative (Photosan-CSAD) nanoparticles were prepared because of their ability to enhance phototoxicity in the photodynamic therapy of pancreatic cancer. These nanoparticles are spherical, 150-250 nm in size as determined by transmission electron microscopy, and have negative zeta potentials. Upon incubation with human pancreatic cancer cells, the Photosan-CSAD nanoparticles showed high fluorescence activity and reactive oxygen species generation, resulting in strong phototoxicity. However, no dark toxicity was observed. Apoptosis played a leading role in the cell death process induced by the Photosan phototoxicity. These results demonstrate that the Photosan-CSAD nanoparticles are a candidate for the photodynamic therapy of pancreatic cancer.
authors.affiliation: Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Institute of Polymer Science, School of Chemistry and Chemical Engineering, Key Laboratory of Designed Synthesis and Application of Polymer Material, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Huajie
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Polymer Science, School of Chemistry and Chemical Engineering, Key Laboratory of Designed Synthesis and Application of Polymer Material, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Institute of Polymer Science, School of Chemistry and Chemical Engineering, Key Laboratory of Designed Synthesis and Application of Polymer Material, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, Guangzhou 510275, China. Electronic address: yanglq@mail.sysu.edu.cn.
authors.forename: Liqun
authors.initials: L
authors.lastname: Yang
mesh_terms: D000464:Alginates / Q000008:administration & dosage* / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000737:chemistry; D006415:Hematoporphyrins / Q000008:administration & dosage / Q000737:chemistry; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D008027:Light; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009336:Necrosis / Q000139:chemically induced; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000464:Alginates; D004337:Drug Carriers; D006415:Hematoporphyrins; D006603:Hexuronic Acids; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; C071875:photosan III; D020723:Glucuronic Acid; D053148:Caspase 3
keywords: Apoptosis; Photosensitizer; Phototoxicity; Reactive oxygen species; Sodium alginate