title: Antimicrobial and Antiproliferative Potential of Anadenanthera colubrina (Vell.) Brenan.
abstract: The aim of the present study was to perform an in vitro analysis of the antimicrobial and antiproliferative potential of an extract from Anadenanthera colubrina (Vell.) Brenan (angico) and chemically characterize the crude extract. Antimicrobial action was evaluated based on the minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration, and the inhibition of formation to oral biofilm. Cell morphology was determined through scanning electron microscopy (SEM). Six strains of tumor cells were used for the determination of antiproliferative potential. The extract demonstrated strong antifungal activity against Candida albicans ATCC 18804 (MIC = 0.031 mg/mL), with similar activity found regarding the ethyl acetate fraction. The extract and active fraction also demonstrated the capacity to inhibit the formation of Candida albicans to oral biofilm after 48 hours, with median values equal to or greater than the control group, but the difference did not achieve statistical significance (P > 0.05). SEM revealed alterations in the cell morphology of the yeast. Regarding antiproliferative activity, the extract demonstrated cytostatic potential in all strains tested. The present findings suggest strong antifungal potential for Anadenanthera colubrina (Vell.) Brenan as well as a tendency toward diminishing the growth of human tumor cells.
authors.affiliation: Department of Dentistry, Paraíba State University (UEPB), 58429-500 Campina Grande, PB, Brazil.
authors.forename: Rennaly de Freitas
authors.initials: Rde F
authors.lastname: Lima
authors.affiliation: Department of Dentistry, Paraíba State University (UEPB), 58429-500 Campina Grande, PB, Brazil.
authors.forename: Erika Ponchet
authors.initials: EP
authors.lastname: Alves
authors.affiliation: Department of Pharmacology and Anesthesiology, Piracicaba School of Dentistry, University of Campinas (UNICAMP), 13414-903 Piracicaba, SP, Brazil.
authors.forename: Pedro Luiz
authors.initials: PL
authors.lastname: Rosalen
authors.affiliation: Center for Chemical, Biological and Agricultural Research, University of Campinas (UNICAMP), P.O. Box 6171, 13083-970 Campinas, SP, Brazil.
authors.forename: Ana Lúcia Tasca Gois
authors.initials: AL
authors.lastname: Ruiz
authors.affiliation: Center for Chemical, Biological and Agricultural Research, University of Campinas (UNICAMP), P.O. Box 6171, 13083-970 Campinas, SP, Brazil.
authors.forename: Marta Cristina
authors.initials: MC
authors.lastname: Teixeira Duarte
authors.affiliation: Center for Chemical, Biological and Agricultural Research, University of Campinas (UNICAMP), P.O. Box 6171, 13083-970 Campinas, SP, Brazil.
authors.forename: Vivian Fernandes Furletti
authors.initials: VF
authors.lastname: Góes
authors.affiliation: Department of Dentistry, Paraíba State University (UEPB), 58429-500 Campina Grande, PB, Brazil.
authors.forename: Ana Cláudia Dantas
authors.initials: AC
authors.lastname: de Medeiros
authors.affiliation: Department of Dentistry, Paraíba State University (UEPB), 58429-500 Campina Grande, PB, Brazil.
authors.forename: Jozinete Vieira
authors.initials: JV
authors.lastname: Pereira
authors.affiliation: Department of Dentistry, Paraíba State University (UEPB), 58429-500 Campina Grande, PB, Brazil.
authors.forename: Gustavo Pina
authors.initials: GP
authors.lastname: Godoy
authors.affiliation: Department of Dentistry, Paraíba State University (UEPB), 58429-500 Campina Grande, PB, Brazil.
authors.forename: Edja Maria
authors.initials: EM
authors.lastname: Melo de Brito Costa
references.citation: Toxicon. 2001 May;39(5):603-13
references.pmid: 11072038
references.citation: J Ethnopharmacol. 2008 Oct 28;119(3):455-61
references.pmid: 18678239
references.citation: J Bacteriol. 2001 Sep;183(18):5385-94
references.pmid: 11514524
references.citation: Int J Antimicrob Agents. 2009 Oct;34(4):333-9
references.pmid: 19376687
references.citation: J Dent (Tehran). 2011 Winter;8(1):1-6
references.pmid: 21998800
references.citation: J Agric Food Chem. 2004 Jun 2;52(11):3583-9
references.pmid: 15161234
references.citation: Nature. 2000 Aug 31;406(6799):959-64
references.pmid: 10984043
references.citation: Microbiol Mol Biol Rev. 1998 Mar;62(1):130-80
references.pmid: 9529890
references.citation: Curr Opin Microbiol. 2006 Dec;9(6):588-94
references.pmid: 17055772
references.citation: J Chem Ecol. 1993 Apr;19(4):613-21
references.pmid: 24249005
references.citation: J Ethnopharmacol. 2008 Oct 30;120(1):72-80
references.pmid: 18725282
references.citation: Food Chem Toxicol. 2006 Oct;44(10):1707-13
references.pmid: 16857303
references.citation: J Ethnobiol Ethnomed. 2006 Mar 23;2:15
references.pmid: 16556305
references.citation: J Nat Prod. 2004 Dec;67(12):2141-53
references.pmid: 15620274
references.citation: Appl Environ Microbiol. 2002 Jun;68(6):2950-8
references.pmid: 12039754
references.citation: J Ethnopharmacol. 2006 Apr 21;105(1-2):173-86
references.pmid: 16298502
references.citation: Can J Microbiol. 2009 Sep;55(9):1033-9
references.pmid: 19898545
references.citation: Int J Artif Organs. 2012 Oct;35(10):780-91
references.pmid: 23138707
references.citation: J Agric Food Chem. 2001 Sep;49(9):4168-70
references.pmid: 11559104
references.citation: Microbiology (Reading). 2003 Feb;149(Pt 2):353-362
references.pmid: 12624197
references.citation: J Ethnopharmacol. 2013 Jun 21;148(1):218-22
references.pmid: 23603196