title: A wheat lipid transfer protein 3 could enhance the basal thermotolerance and oxidative stress resistance of Arabidopsis.
abstract: Wheat (Triticum aestivum L.) is one of the major grain crops, and heat stress adversely affects wheat production in many regions of the world. Previously, we found a heat-responsive gene named Lipid Transfer Protein 3 (TaLTP3) in wheat. TaLTP3 was deduced to be regulated by cold, ABA, MeJA, Auxin and oxidative stress according to cis-acting motifs in its promoter sequences. In this study, we show that TaLTP3 is responsive to prolonged water deficit, salt or ABA treatment in wheat seedlings. Also, TaLTP3 accumulation was observed after the plant suffered from heat stress both at the seedling and the grain-filling stages. TaLTP3 protein was localized in the cell membrane and cytoplasm of tobacco epidermal cells. Overexpression of TaLTP3 in yeast imparted tolerance to heat stress compared to cells expressing the vector alone. Most importantly, transgenic Arabidopsis plants engineered to overexpress TaLTP3 showed higher thermotolerance than control plants at the seedling stage. Further investigation indicated that transgenic lines decreased H₂O₂ accumulation and membrane injury under heat stress. Taken together, our results demonstrate that TaLTP3 confers heat stress tolerance possibly through reactive oxygen species (ROS) scavenging.
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Centre (Beijing), Beijing 100193, China. Electronic address: wangfeihh@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Centre (Beijing), Beijing 100193, China. Electronic address: dulzxs@163.com.
authors.forename: Xin-shan
authors.initials: XS
authors.lastname: Zang
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Centre (Beijing), Beijing 100193, China. Electronic address: rezaulw@yahoo.com.
authors.forename: Muhammad Rezaul
authors.initials: MR
authors.lastname: Kabir
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Centre (Beijing), Beijing 100193, China. Electronic address: liukelu731@gmail.com.
authors.forename: Ke-lu
authors.initials: KL
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Centre (Beijing), Beijing 100193, China. Electronic address: shanzhenliu@163.com.
authors.forename: Zhen-shan
authors.initials: ZS
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Centre (Beijing), Beijing 100193, China. Electronic address: wheat3392@cau.edu.cn.
authors.forename: Zhong-fu
authors.initials: ZF
authors.lastname: Ni
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Centre (Beijing), Beijing 100193, China. Electronic address: yaoyingyin@163.com.
authors.forename: Ying-yin
authors.initials: YY
authors.lastname: Yao
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Centre (Beijing), Beijing 100193, China. Electronic address: hzr803@163.com.
authors.forename: Zhao-rong
authors.initials: ZR
authors.lastname: Hu
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Centre (Beijing), Beijing 100193, China. Electronic address: qxsun@cau.edu.cn.
authors.forename: Qi-xin
authors.initials: QX
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Centre (Beijing), Beijing 100193, China. Electronic address: penghuiru@cau.edu.cn.
authors.forename: Hui-ru
authors.initials: HR
authors.lastname: Peng
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D019441:Plant Epidermis / Q000166:cytology / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D002352:Carrier Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C030363:lipid transfer protein; D049452:Green Fluorescent Proteins; D000040:Abscisic Acid; D006861:Hydrogen Peroxide
keywords: Antioxidant; Thermotolerance; Wheat lipid transfer protein 3