title: Molecular characterization of a Spirometra mansoni antigenic polypeptide gene encoding a 28.7 kDa protein.
abstract: The Spirometra mansoni antigenic polypeptide (SmAP) gene was expressed in Escherichia coli, and its characteristics and value as an antigen for the serodiagnosis of sparganosis were investigated. The recombinant SmAP protein (rSmAP) has the molecular weight of 28.7 kDa. On Western blotting analysis, the rSmAP strongly reacted with the sera of mice infected with spargana, but not with normal sera; the anti-rSmAP serum obviously recognized the 28.7-kDa band in the crude antigens and excretory-secretory (ES) antigens of spargana. The immunofluorescence test (IFT) results showed that the positive staining was observed at different stages of spargana from the infected frogs and mice, but not adult worm of S. mansoni. An immunolocalization analysis identified SmAP in the teguments and parenchymal tissues of spargana. ELISA with rSmAP antigen or sparganum ES antigens were evaluated for the serodiagnosis of sparganosis. The results showed that the sensitivity of rSmAP-ELISA and ES-ELISA was 83.3% (25/30) and 100% (30/30), respectively, for the detection of anti-sparganum IgG antibodies in sera of the experimentally infected mice (P > 0.05), the specificities of both ELISA were 100% (67/67). It is suggested that the rSmAP might be a potential candidate antigen for serodiagnosis of sparganosis.
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou, 450052, People's Republic of China, cuij@zzu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
authors.forename: Tong
authors.initials: T
authors.lastname: Wei
authors.forename: Li Na
authors.initials: LN
authors.lastname: Liu
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.forename: Zi Fang
authors.initials: ZF
authors.lastname: Zhang
authors.forename: Zhong Quan
authors.initials: ZQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000378:metabolism*; D002590:Cestode Infections / Q000175:diagnosis / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000378:metabolism; D012698:Serologic Tests; D013146:Spirometra / Q000378:metabolism*
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins
references.citation: Parasit Vectors. 2013 Aug 23;6:246
references.pmid: 23972034
references.citation: Parasitology. 1994 Dec;109 ( Pt 5):611-21
references.pmid: 7831096
references.citation: Trans R Soc Trop Med Hyg. 1994 Nov-Dec;88(6):663-5
references.pmid: 7886764
references.citation: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(1):39-42
references.pmid: 12563815
references.citation: Vaccine. 2013 Feb 6;31(8):1171-7
references.pmid: 23306358
references.citation: Emerg Infect Dis. 2011 Jan;17(1):146-7
references.pmid: 21192885
references.citation: Iran J Parasitol. 2013 Jul;8(3):408-16
references.pmid: 24454434
references.citation: Int J Food Microbiol. 2009 Sep 15;134(3):163-75
references.pmid: 19679367
references.citation: Parasitol Res. 2011 Mar;108(3):593-9
references.pmid: 20922411
references.citation: Korean J Parasitol. 2014 Feb;52(1):41-6
references.pmid: 24623880
references.citation: Virol J. 2008 Jun 06;5:74
references.pmid: 18538016
references.citation: Exp Parasitol. 2013 Jun;134(2):148-54
references.pmid: 23501807
references.citation: Vet Parasitol. 2004 Dec 9;126(1-2):167-93
references.pmid: 15567584
references.citation: Acta Trop. 2011 Jun;118(3):171-6
references.pmid: 21459073
references.citation: Parasitol Today. 1999 Mar;15(3):124
references.pmid: 10323750
references.citation: Surg Neurol. 2007 Apr;67(4):409-11; discussion 412
references.pmid: 17350417
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Parasitol Res. 2009 Nov;105(6):1549-56
references.pmid: 19685078
references.citation: Southeast Asian J Trop Med Public Health. 2014 May;45(3):576-83
references.pmid: 24974641
references.citation: Parasitol Res. 2011 Jun;108(6):1551-6
references.pmid: 21181193