title: Multifunctionalized reduced graphene oxide-doped polypyrrole/pyrrolepropylic acid nanocomposite impedimetric immunosensor to ultra-sensitively detect small molecular aflatoxin B₁.
abstract: Aflatoxin B1 (AFB1), an aflatoxin is extremely toxic among mycotoxins in contaminated food products but it is very difficult to be quantitatively detected by existing methods. Impedimetric immunosensor is an advantageously label-free and fast assay. Nevertheless, its applications are limited by low sensitivity when the target molecule is small such as AFB1 due to relatively low impedance change during detection. Herein for the first time reduced graphene oxide (rGO) is nanocomposed with polypyrrole (PPy) and pyrrolepropylic acid (PPa) as a unique sensing platform, in which rGO greatly improves the conductivity and stability, PPa provides covalent linkers for probe immobilization and PPy endows the film electroactivity from its inherent electrochemical doping/dedoping property for impedance measurements, thus significantly improving the sensitivity to detect AFB1 in a range of 10 fg mL(-1) to 10 pg mL(-1) with high specificity and good reproducibility. This work demonstrates a novel method to sensitively detect small molecule by using immunoassay.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory Institute for Advanced Materials & Technologies of Clean Energies, Chongqing 400715, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: xuyang1951@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory Institute for Advanced Materials & Technologies of Clean Energies, Chongqing 400715, China. Electronic address: ecmli@swu.edu.cn.
authors.forename: Chang
authors.initials: C
authors.lastname: Ming Li
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation; D005506:Food Contamination / Q000032:analysis; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D008970:Molecular Weight; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D010087:Oxides; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D006108:Graphite; D016604:Aflatoxin B1
keywords: Aflatoxin B(1); Impedimetric immunosensor; Polypyrrole/pyrrolepropylic acid; Reduced graphene oxide