title: Asymmetric division and differential gene expression during a bacterial developmental program requires DivIVA.
abstract: Sporulation in the bacterium Bacillus subtilis is a developmental program in which a progenitor cell differentiates into two different cell types, the smaller of which eventually becomes a dormant cell called a spore. The process begins with an asymmetric cell division event, followed by the activation of a transcription factor, σF, specifically in the smaller cell. Here, we show that the structural protein DivIVA localizes to the polar septum during sporulation and is required for asymmetric division and the compartment-specific activation of σF. Both events are known to require a protein called SpoIIE, which also localizes to the polar septum. We show that DivIVA copurifies with SpoIIE and that DivIVA may anchor SpoIIE briefly to the assembling polar septum before SpoIIE is subsequently released into the forespore membrane and recaptured at the polar septum. Finally, using super-resolution microscopy, we demonstrate that DivIVA and SpoIIE ultimately display a biased localization on the side of the polar septum that faces the smaller compartment in which σF is activated.
authors.affiliation: Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Prahathees
authors.initials: P
authors.lastname: Eswaramoorthy
authors.affiliation: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Peter W
authors.initials: PW
authors.lastname: Winter
authors.affiliation: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Peter
authors.initials: P
authors.lastname: Wawrzusin
authors.affiliation: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: York
authors.affiliation: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Hari
authors.initials: H
authors.lastname: Shroff
authors.affiliation: Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Kumaran S
authors.initials: KS
authors.lastname: Ramamurthi
mesh_terms: D060049:Asymmetric Cell Division / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D016764:Cell Polarity; D015964:Gene Expression Regulation, Bacterial; D012808:Sigma Factor / Q000235:genetics*; D013171:Spores, Bacterial / Q000235:genetics* / Q000254:growth & development
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; C104636:DivIVA protein, bacteria; D012808:Sigma Factor; C030069:spore-specific proteins, Bacillus
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