title: Selective naked-eye detection of Hg²⁺ through an efficient turn-on photoinduced electron transfer fluorescent probe and its real applications.
abstract: A simple molecular fluorescent probe 5 has been designed and synthesized by appending anthracene and benzhydryl moieties through a piperazine bridge. The probe upon interaction with different metal ions showed high selectivity and sensitivity (2 ppb) for Hg(2+) through fluorescence "turn-on" response in HEPES buffer. The significant fluorescence enhancement (~10-fold) is attributable to PET arrest due to complexation with nitrogen atoms of the piperazine unit and Hg(2+) in 1:2 stoichiometry, in which a naked-eye sensitive fluorescent blue color of solution changed to a blue-green (switched-on). As a proof of concept, promising prospects for application in environmental and biological sciences 5 have been utilized to detect Hg(2+) sensitively in real samples, on cellulose paper strips, in protein medium (like BSA), and intracellularly in HeLa cells. Moreover, the optical behavior of 5 upon providing different chemical inputs has been utilized to construct individual logic gates and a reusable combinational logic circuit. The combinational circuit (switch ON mode; OR logic gate) is easily resettable to the original position (switch OFF mode; INHIBIT logic gate) by applying reset chemical inputs (OH(-) and PO4(3-)) with great reproducibility.
authors.affiliation: Department of Chemistry, Faculty of Science, Banaras Hindu University , Varanasi 221005 Uttar Pradesh, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Srivastava
authors.forename: Syed S
authors.initials: SS
authors.lastname: Razi
authors.forename: Rashid
authors.initials: R
authors.lastname: Ali
authors.forename: Ramesh C
authors.initials: RC
authors.lastname: Gupta
authors.forename: Suresh S
authors.initials: SS
authors.lastname: Yadav
authors.forename: Gopeshwar
authors.initials: G
authors.lastname: Narayan
authors.forename: Arvind
authors.initials: A
authors.lastname: Misra
mesh_terms: D000818:Animals; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D003124:Colorimetry; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007477:Ions / Q000737:chemistry; D008027:Light; D008628:Mercury / Q000032:analysis*; D018613:Microscopy, Confocal; D000077489:Piperazine; D010879:Piperazines / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D005456:Fluorescent Dyes; D007477:Ions; D010879:Piperazines; D012709:Serum Albumin; D014874:Water Pollutants, Chemical; D000077489:Piperazine; D008628:Mercury