title: Protective Effect of Lavandula stoechas and Rosmarinus officinalis essential oils against reproductive damage and oxidative stress in alloxan-induced diabetic rats.
abstract: The authors aimed in the present study to assess the protective effect of Rosmarinus officinalis essential oils (ROEO) and Lavandula stoechas essential oils (LSEO) against reproductive damage and oxidative stress in alloxan-induced diabetic male rats. Essential oil samples were obtained from the aerial parts of the plants by hydrodistillation and analyzed by the gas chromatography-mass spectrometry (GC-MS). Rats were divided into four groups: healthy control (HC); diabetic control (DC); healthy+ROEO (H+ROEO), healthy+LSEO (H+LSEO), diabetic+ROEO (D+ROEO), and diabetic+LSEO (D+LSEO). The use of GC-MS allowed to the identification of 15 and 22 compounds in ROEO and LSEO, respectively. In addition, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test showed that ROEO and LSEO had an important antioxidant capacity. In vivo, we initially found that ROEO and LSEO treatment protected against the decrease in alloxan-induced body weight gain, relative reproductive organ weights, testosterone level, as well as sperm quality decline. On the other hand, we showed that alloxan administration was accompanied by an oxidative stress status assessed by an increase of malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels, as well as a depletion of sulfhydril group content (-SH) and antioxidant enzyme activities as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in testis, epididymis, and sperm. More importantly, ROEO and LSEO treatment significantly protected against oxidative damage of the male reproductive organ systems in alloxan-induced diabetic rats. These findings suggested that ROEO and LSEO exerted a potential protective effect against alloxan-induced reproductive function damage and oxidative stress in male rat. The beneficial effect of ROEO and LSEO might be related, in part, to their antioxidant properties.
authors.affiliation: 1 Laboratoire de Physiologie Intégrée , Faculté des Sciences de Bizerte, Zarzouna, Tunisia .
authors.forename: Hichem
authors.initials: H
authors.lastname: Sebai
authors.forename: Slimen
authors.initials: S
authors.lastname: Selmi
authors.forename: Kais
authors.initials: K
authors.lastname: Rtibi
authors.forename: Najoua
authors.initials: N
authors.lastname: Gharbi
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
mesh_terms: D000496:Alloxan; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002374:Catalase; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004822:Epididymis / Q000201:enzymology; D005979:Glutathione Peroxidase; D006861:Hydrogen Peroxide / Q000097:blood; D027523:Lavandula / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009822:Oils, Volatile / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D027542:Rosmarinus / Q000737:chemistry*; D055101:Semen Analysis; D013094:Spermatozoa / Q000201:enzymology; D013438:Sulfhydryl Compounds / Q000097:blood; D013482:Superoxide Dismutase; D013737:Testis / Q000201:enzymology; D013739:Testosterone / Q000097:blood
chemical_list: D000975:Antioxidants; D009822:Oils, Volatile; D010938:Plant Oils; D013438:Sulfhydryl Compounds; D013739:Testosterone; D008315:Malondialdehyde; D000496:Alloxan; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Lavandula stoechas; Rosmarinus officinalis; acetylcholinestrase; essentials oils; oxidative stress; rat; testosterone