title: Development and characterization of salivary adenoid cystic carcinoma cell line.
abstract: OBJECTIVE
  To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies.
  
  
  METHODS
  Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.
  
  
  RESULTS
  Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.
  
  
  CONCLUSIONS
  We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.
authors.affiliation: The University of Texas, M.D. Anderson Cancer Center, Sections of Head and Neck Pathology, United States.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Baylor College of Medicine, Department of Pediatrics, Texas Children's Cancer and Hematology Centers, United States.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Perlaky
authors.affiliation: Baylor College of Medicine, Department of Pediatrics, Texas Children's Cancer and Hematology Centers, United States.
authors.forename: Pulivarthi
authors.initials: P
authors.lastname: Rao
authors.affiliation: The Department of Head and Neck Surgery, M.D. Anderson Cancer Center, United States.
authors.forename: Randal S
authors.initials: RS
authors.lastname: Weber
authors.affiliation: The University of Texas, M.D. Anderson Cancer Center, Sections of Head and Neck Pathology, United States. Electronic address: anaggar@mdanderson.org.
authors.forename: Adel K
authors.initials: AK
authors.lastname: El-Naggar
mesh_terms: D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D016895:Culture Media, Serum-Free; D017931:DNA Primers; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018895:Microsatellite Repeats; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019098:Telomerase / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D016895:Culture Media, Serum-Free; D017931:DNA Primers; C509186:TERT protein, human; D019098:Telomerase
keywords: Adenoid cystic carcinoma; Cell line; Salivary gland tumors
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