title: Integration of binding peptide selection and multifunctional particles as tool-box for capture of soluble proteins in serum.
abstract: In this paper, we report on a general approach for the detection of a specific tumoural biomarker directly in serum. Such detection is made possible using a protein-binding peptide selected through an improved phage display technique and then conjugated to engineered microparticles (MPs). Protein biomarkers represent an unlimited source of information for non-invasive diagnostic and prognostic tests; MP-based assays are becoming largely used in manipulation of soluble biomarkers, but their direct use in serum is hampered by the complex biomolecular environment. Our technique overcomes the current limitations as it produces a selective MP--engineered with an antifouling layer--that 'captures' the relevant protein staying impervious to the background. Our system succeeds in fishing-out the human tumour necrosis factor alpha directly in serum with a high selectivity degree. Our method could have great impact in soluble protein manipulation and detection for a wide variety of diagnostic applications.
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy.
authors.forename: Angela Maria
authors.initials: AM
authors.lastname: Cusano
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples 'Federico II', Naples, Italy Department of Chemical and Materials Engineering and Industrial Production, University of Naples 'Federico II', Naples, Italy causa@unina.it.
authors.forename: Filippo
authors.initials: F
authors.lastname: Causa
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy.
authors.forename: Raffaella Della
authors.initials: RD
authors.lastname: Moglie
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy.
authors.forename: Nunzia
authors.initials: N
authors.lastname: Falco
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy.
authors.forename: Pasqualina Liana
authors.initials: PL
authors.lastname: Scognamiglio
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Aliberti
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Vecchione
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy.
authors.forename: Edmondo
authors.initials: E
authors.lastname: Battista
authors.affiliation: Department of Pharmacy, CIRPEB: Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, University of Naples 'Federico II', DFM-Scarl, Naples, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Marasco
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy Department of Chemical Science, University of Naples 'Federico II', Naples, Italy.
authors.forename: Marika
authors.initials: M
authors.lastname: Savarese
authors.affiliation: Department of Chemical Science, University of Naples 'Federico II', Naples, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Raucci
authors.affiliation: Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples 'Federico II', Naples, Italy Department of Chemical Science, University of Naples 'Federico II', Naples, Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Rega
authors.affiliation: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples 'Federico II', Naples, Italy Department of Chemical and Materials Engineering and Industrial Production, University of Naples 'Federico II', Naples, Italy.
authors.forename: Paolo Antonio
authors.initials: PA
authors.lastname: Netti
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002151:Calorimetry; D061505:Cell Surface Display Techniques; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D006801:Humans; D046210:Microfluidic Analytical Techniques; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000302:isolation & purification
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha
keywords: microparticles for diagnosis; phage display selection; soluble cancer biomarker
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