title: The Os-AKT1 channel is critical for K+ uptake in rice roots and is modulated by the rice CBL1-CIPK23 complex.
abstract: Potassium (K(+)) is one of the essential nutrient elements for plant growth and development. Plants absorb K(+) ions from the environment via root cell K(+) channels and/or transporters. In this study, the Shaker K(+) channel Os-AKT1 was characterized for its function in K(+) uptake in rice (Oryza sativa) roots, and its regulation by Os-CBL1 (Calcineurin B-Like protein1) and Os-CIPK23 (CBL-Interacting Protein Kinase23) was investigated. As an inward K(+) channel, Os-AKT1 could carry out K(+) uptake and rescue the low-K(+)-sensitive phenotype of Arabidopsis thaliana akt1 mutant plants. Rice Os-akt1 mutant plants showed decreased K(+) uptake and displayed an obvious low-K(+)-sensitive phenotype. Disruption of Os-AKT1 significantly reduced the K(+) content, which resulted in inhibition of plant growth and development. Similar to the AKT1 regulation in Arabidopsis, Os-CBL1 and Os-CIPK23 were identified as the upstream regulators of Os-AKT1 in rice. The Os-CBL1-Os-CIPK23 complex could enhance Os-AKT1-mediated K(+) uptake. A phenotype test confirmed that Os-CIPK23 RNAi lines exhibited similar K(+)-deficient symptoms as the Os-akt1 mutant under low K(+) conditions. These findings demonstrate that Os-AKT1-mediated K(+) uptake in rice roots is modulated by the Os-CBL1-Os-CIPK23 complex.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Long
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China.
authors.forename: Guo-Ning
authors.initials: GN
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China.
authors.forename: Zi-Jian
authors.initials: ZJ
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, China yiwang@cau.edu.cn.
authors.forename: Yi
authors.identifier: http://orcid.org/0000-0002-3660-5859
authors.initials: Y
authors.lastname: Wang
mesh_terms: D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005816:Genetic Complementation Test; D012275:Oryza / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018517:Plant Roots / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D014026:Nicotiana / Q000235:genetics; D015003:Yeasts / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D002135:Calcium-Binding Proteins; D010940:Plant Proteins; D015221:Potassium Channels; C074500:AKT1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; D011188:Potassium
references.citation: Plant J. 2013 Mar;73(6):1006-18
references.pmid: 23217029
references.citation: J Exp Bot. 2006;57(2):425-36
references.pmid: 16364949
references.citation: FEBS Lett. 2001 Nov 23;508(3):463-9
references.pmid: 11728473
references.citation: CSH Protoc. 2008 Apr 01;2008:pdb.prot4995
references.pmid: 21356813
references.citation: Plant J. 2010 Jan;61(1):58-69
references.pmid: 19781051
references.citation: Plant J. 2008 Jan;53(1):115-23
references.pmid: 17976154
references.citation: Pflugers Arch. 1981 Aug;391(2):85-100
references.pmid: 6270629
references.citation: Planta. 2005 May;221(2):212-21
references.pmid: 15599592
references.citation: Planta. 1984 May;160(6):490-9
references.pmid: 24258775
references.citation: Plant J. 1996 Feb;9(2):195-203
references.pmid: 8820606
references.citation: Plant Physiol. 2004 Nov;136(3):3824-37
references.pmid: 15489280
references.citation: Plant Physiol. 2005 Mar;137(3):1105-14
references.pmid: 15734909
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Plant Physiol. 2011 Jul;156(3):1493-507
references.pmid: 21610181
references.citation: Plant Physiol. 1998 Mar;116(3):879-90
references.pmid: 9501121
references.citation: Plant Cell. 1998 Jan;10(1):51-62
references.pmid: 9477571
references.citation: Plant J. 2004 Nov;40(3):428-38
references.pmid: 15469500
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12625-30
references.pmid: 16895985
references.citation: EMBO J. 2007 Jun 20;26(12):3003-14
references.pmid: 17541409
references.citation: J Bacteriol. 1984 Sep;159(3):940-5
references.pmid: 6384187
references.citation: Plant Physiol. 2004 Mar;134(3):1135-45
references.pmid: 14988478
references.citation: J Biol Chem. 1997 Jan 10;272(2):1011-8
references.pmid: 8995396
references.citation: Plant Mol Biol. 2003 Jan;51(1):71-81
references.pmid: 12602892
references.citation: Plant Physiol. 2000 Apr;122(4):1387-97
references.pmid: 10759535
references.citation: Planta. 2000 Apr;210(5):723-31
references.pmid: 10805443
references.citation: Plant J. 2008 Nov;56(3):505-16
references.pmid: 18643980
references.citation: J Gen Physiol. 1999 Jun;113(6):909-18
references.pmid: 10352038
references.citation: Plant J. 2007 Dec;52(5):891-8
references.pmid: 17908158
references.citation: Mol Cell Biol. 1988 Jul;8(7):2848-59
references.pmid: 3043197
references.citation: Plant Cell. 2008 May;20(5):1346-62
references.pmid: 18502848
references.citation: J Biol Chem. 2009 Aug 7;284(32):21288-95
references.pmid: 19509299
references.citation: Cell Res. 2010 Jul;20(7):826-37
references.pmid: 20514083
references.citation: J Genet Genomics. 2008 Sep;35(9):531-43, S1-2
references.pmid: 18804072
references.citation: Plant Cell Environ. 2009 Dec;32(12):1761-77
references.pmid: 19682291
references.citation: Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):12186-91
references.pmid: 10518597
references.citation: Plant Mol Biol. 2003 Mar;51(5):773-87
references.pmid: 12678562
references.citation: Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):4079-84
references.pmid: 11904452
references.citation: Annu Rev Plant Biol. 2013;64:451-76
references.pmid: 23330792
references.citation: Annu Rev Plant Biol. 2007;58:47-69
references.pmid: 17067284
references.citation: Plant J. 2011 Aug;67(4):570-82
references.pmid: 21518051
references.citation: Cell. 2006 Jun 30;125(7):1347-60
references.pmid: 16814720
references.citation: Science. 1998 May 8;280(5365):918-21
references.pmid: 9572739
references.citation: Plant Physiol. 2002 Oct;130(2):784-95
references.pmid: 12376644
references.citation: Plant Physiol. 2010 Jan;152(1):341-55
references.pmid: 19889878
references.citation: Curr Opin Plant Biol. 2009 Jun;12(3):339-46
references.pmid: 19501014
references.citation: Plant J. 2003 Sep;35(6):675-92
references.pmid: 12969422
references.citation: Science. 1998 Apr 3;280(5360):69-77
references.pmid: 9525859
references.citation: Plant Cell. 2009 Aug;21(8):2485-502
references.pmid: 19700632