title: Basics for the preparation of quantum dots and their interactions with living cells.
abstract: A study of the interactions between nanoparticles and living cells is invaluable in understanding the nano-biological effect and the mechanism of cellular endocytosis. Here we describe two methods for the preparation of semiconductor quantum dots with different physiochemical properties. Furthermore, we describe how to study the interaction of the two quantum dots with living HeLa cells and red blood cells with confocal microscopy.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625, Ren Min Street, Changchun, 130022, China, jiangxiue@ciac.ac.cn.
authors.forename: Xiue
authors.initials: X
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.forename: Tiantian
authors.initials: T
authors.lastname: Wang
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D018613:Microscopy, Confocal; D036103:Nanotechnology / Q000379:methods*; D010396:Penicillamine / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry; D013870:Thiomalates / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D008024:Ligands; D013440:Sulfides; D013870:Thiomalates; D017967:Zinc Compounds; C046062:2-thiomalic acid; D010396:Penicillamine; C031238:zinc sulfide; D013691:Tellurium; C028337:cadmium telluride