title: Serotonin regulates osteoblast proliferation and function in vitro.
abstract: The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter, also has important functions outside the central nervous system. The objective of this study was to investigate the role of 5-HT in the proliferation, differentiation, and function of osteoblasts in vitro. We treated rat primary calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and assessed the rate of osteoblast proliferation, expression levels of osteoblast-specific proteins and genes, and the ability to form mineralized nodules. Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at different stages of osteoblast differentiation. We found that 5-HT could inhibit osteoblast proliferation, differentiation, and mineralization at low concentrations, but this inhibitory effect was mitigated at relatively high concentrations. Six of the 5-HT receptor subtypes (5-HT1A, 5-HT1B, 5-HT1D, 5-HT2A, 5-HT2B, and 5-HT2C) were found to exist in rat osteoblasts. Of these, 5-HT2A and 5-HT1B receptors had the highest expression levels, at both early and late stages of differentiation. Our results indicated that 5-HT can regulate osteoblast proliferation and function in vitro.
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Dai
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: L P
authors.initials: LP
authors.lastname: Yu
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: X
authors.initials: X
authors.lastname: Shi
authors.affiliation: Emergency Department, The First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: P
authors.initials: P
authors.lastname: Shao
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: G Y
authors.initials: GY
authors.lastname: Yin
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Wei
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D017931:DNA Primers; D015870:Gene Expression; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D061251:Primary Cell Culture; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D011985:Receptors, Serotonin / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D011985:Receptors, Serotonin; D012701:Serotonin
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