title: A sensitive and specific molecular tool for detection of both typical and atypical enteroaggregative Escherichia coli.
abstract: A multiplex PCR has been standardized and evaluated for the identification of both typical and atypical enteroaggregative Escherichia coli (EAEC). The assay detecting aaiA, aaiG, aggR and aatA presented 94.8% of sensitivity, 94.3% of specificity and was able to efficiently detect both groups of EAEC among E. coli isolated from stool cultures.
authors.affiliation: Laboratório de Bacteriologia, Instituto Butantan, São Paulo, Brazil.
authors.forename: Fernanda B
authors.initials: FB
authors.lastname: Andrade
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Tânia A T
authors.initials: TAT
authors.lastname: Gomes
authors.affiliation: Laboratório de Bacteriologia, Instituto Butantan, São Paulo, Brazil. Electronic address: waldir.elias@butantan.gov.br.
authors.forename: Waldir P
authors.initials: WP
authors.lastname: Elias
mesh_terms: D000328:Adult; D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D005243:Feces / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
keywords: EAEC; Enteroaggregative Escherichia coli; Multiplex PCR; Sensitivity; Specificity