title: Nature's recipe for splitting inteins.
abstract: Protein splicing in trans by split inteins has increasingly become a powerful protein-engineering tool for protein ligation, both in vivo and in vitro. Over 100 naturally occurring and artificially engineered split inteins have been reported for protein ligation using protein trans-splicing. Here, we review the current status of the reported split inteins in order to delineate an empirical or rational strategy for constructing new split inteins suitable for various applications in biotechnology and chemical biology.
authors.affiliation: Research Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, P.O. Box 65, Helsinki FIN-00014, Finland.
authors.forename: A Sesilja
authors.initials: AS
authors.lastname: Aranko
authors.affiliation: Macromolecular Crystallography Laboratory, National Cancer Institute-Frederick, MD 21702, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wlodawer
authors.affiliation: Research Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, P.O. Box 65, Helsinki FIN-00014, Finland hideo.iwai@helsinki.fi.
authors.forename: Hideo
authors.initials: H
authors.lastname: Iwaï
mesh_terms: D000818:Animals; D006801:Humans; D047668:Inteins; D008958:Models, Molecular; D019154:Protein Splicing; D020040:Trans-Splicing
keywords: protein ligation; protein splicing; protein trans-splicing; split intein
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