title: The Golgi ribbon structure facilitates anterograde transport of large cargoes.
abstract: In mammalian cells, individual Golgi stacks fuse laterally to form the characteristic perinuclear ribbon structure. Yet the purpose of this remarkable structure has been an enigma. We report that breaking down the ribbon of mammalian cells strongly inhibits intra-Golgi transport of large cargoes without altering the rate of transport of smaller cargoes. In addition, insect cells that naturally harbor dispersed Golgi stacks have limited capacity to transport artificial oversized cargoes. These results imply that the ribbon structure is an essential requirement for transport of large cargoes in mammalian cells, and we suggest that this is because it enables the dilated rims of cisternae (containing the aggregates) to move across the stack as they transfer among adjacent stacks within the ribbon structure.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520 gregory.lavieu@yale.edu james.rothman@yale.edu.
authors.forename: Gregory
authors.initials: G
authors.lastname: Lavieu
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520.
authors.forename: Myun Hwa
authors.initials: MH
authors.lastname: Dunlop
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520.
authors.forename: Alexander
authors.initials: A
authors.lastname: Lerich
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520.
authors.forename: Hong
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520.
authors.forename: Francesca
authors.initials: F
authors.lastname: Bottanelli
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520 gregory.lavieu@yale.edu james.rothman@yale.edu.
authors.forename: James E
authors.initials: JE
authors.lastname: Rothman
mesh_terms: D000818:Animals; D001692:Biological Transport; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000378:metabolism*; D004330:Drosophila; D006056:Golgi Apparatus / Q000378:metabolism*; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D015739:Nocodazole / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering; D050257:Tubulin Modulators / Q000494:pharmacology
chemical_list: D024042:Collagen Type I; C108705:GORASP1 protein, human; C122048:GORASP2 protein, human; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D034741:RNA, Small Interfering; D050257:Tubulin Modulators; D015739:Nocodazole
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