title: Butanol production by immobilised Clostridium acetobutylicum in repeated batch, fed-batch, and continuous modes of fermentation.
abstract: Clostridium acetobutylicum immobilised in polyvinylalcohol, lens-shaped hydrogel capsules (LentiKats(®)) was studied for production of butanol and other products of acetone-butanol-ethanol fermentation. After optimising the immobilisation protocol for anaerobic bacteria, continuous, repeated batch, and fed-batch fermentations in repeated batch mode were performed. Using glucose as a substrate, butanol productivity of 0.41 g/L/h and solvent productivity of 0.63 g/L/h were observed at a dilution rate of 0.05 h(-1) during continuous fermentation with a concentrated substrate (60 g/L). Through the process of repeated batch fermentation, the duration of fermentation was reduced from 27.8h (free-cell fermentation) to 3.3h (immobilised cells) with a solvent productivity of 0.77 g/L/h (butanol 0.57 g/L/h). The highest butanol and solvent productivities of 1.21 and 1.91 g/L/h were observed during fed-batch fermentation operated in repeated batch mode with yields of butanol (0.15 g/g) and solvents (0.24 g/g), respectively, produced per gram of glucose.
authors.affiliation: Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia.
authors.forename: Igor
authors.initials: I
authors.lastname: Dolejš
authors.affiliation: Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Krasňan
authors.affiliation: LentiKat's a.s., Pod Vinicí 83, 471 27 Stráž pod Ralskem, Czech Republic.
authors.forename: Radek
authors.initials: R
authors.lastname: Stloukal
authors.affiliation: Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia.
authors.forename: Michal
authors.initials: M
authors.lastname: Rosenberg
authors.affiliation: Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia. Electronic address: martin.rebros@stuba.sk.
authors.forename: Martin
authors.initials: M
authors.lastname: Rebroš
mesh_terms: D000096:Acetone / Q000378:metabolism; D000693:Anaerobiosis; D061249:Batch Cell Culture Techniques / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D018914:Cells, Immobilized / Q000378:metabolism; D046969:Clostridium acetobutylicum / Q000166:cytology / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation
chemical_list: D000440:Butanols; D000096:Acetone; D000431:Ethanol
keywords: Butanol; Clostridium acetobutylicum; Fermentation; Immobilisation; LentiKats(®)