title: Minocycline encapsulated chitosan nanoparticles for central antinociceptive activity.
abstract: The purpose of the study is to explore the central anti-nociceptive activity of brain targeted nanoparticles (NP) of minocycline hydrochloride (MH). The NP were formulated using the modified ionotropic gelation method (MHNP) and were coated with Tween 80 (T80) to target them to brain (cMHNP). The formulated nanoparticles have already been characterized for particle size, zeta potential, drug entrapment efficiency and in vitro drug release. The nanoparticles were then evaluated for pharmacodynamic activity using thermal methods. The pure drug and the formulation, MHNP were not able to show a statistically significant central analgesic activity. cMHNP on the other hand evidenced a significant central analgesic activity. Animal models evidenced that brain targeted nanoparticles may be utilized for effective delivery of central anti-nociceptive effect of MH. Further clinical studies are required to explore the activity for mankind.
authors.affiliation: Division of Research and Development, Lovely Professional University, Punjab 144411, India. Electronic address: kalpana.18082@lpu.co.in.
authors.forename: Kalpana
authors.initials: K
authors.lastname: Nagpal
authors.affiliation: Department of Pharmaceutical Sciences, Guru Jambeshwar University of Science & Technology, Hisar, Haryana, 125001, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Department of Pharmaceutical Sciences, Guru Jambeshwar University of Science & Technology, Hisar, Haryana, 125001, India.
authors.forename: D N
authors.initials: DN
authors.lastname: Mishra
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects*; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000737:chemistry*; D007101:Immersion; D008297:Male; D051379:Mice; D008911:Minocycline / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011930:Reaction Time / Q000187:drug effects; D012018:Reflex / Q000187:drug effects; D055672:Static Electricity; D013623:Tail; D013997:Time Factors
chemical_list: D000700:Analgesics; D048271:Chitosan; D008911:Minocycline
keywords: Hot plate test; Minocycline; Tail immersion test