title: Changes in the distribution of type II transmembrane serine protease, TMPRSS2 and in paracellular permeability in IPEC-J2 cells exposed to oxidative stress.
abstract: The effect of oxidative stress on barrier integrity and localization of transmembrane serine proteinase 2 (TMPRSS2) were studied using porcine epithelial IPEC-J2 cells on membrane inserts. Increased paracellular permeability of FITC-dextran 4 kDa (fluorescence intensity 43,508 ± 2,391 versus 3,550 ± 759) and that of gentamicin (3.41 ± 0.06 % increase to controls) were measured parallel with the reduced transepithelial electrical resistance (23.3 ± 4.06 % decrease) of cell layers 6 h after 1 h 1 mM H2O2 treatment. The immunohistochemical localization of adherens junctional β-catenin was not affected by reactive oxygen species (ROS) up to 4 mM H2O2. Peroxide-triggered enhanced paracellular permeability of IPEC-J2 cell layer was accompanied by predominantly cytoplasmic occurrence of TMPRSS2 embedded in cell membrane under physiological conditions. These results support that ROS can influence paracellular gate opening via multifaceted mode of action without involvement of β-catenin redistribution in adherens junction. Altered distribution pattern of TMPRSS2 and relocalized transmembrane serine protease activity may contribute to weakening of epithelial barrier integrity under acute oxidative stress.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest, 1078, Hungary, Gere.Erzsebet@aotk.szie.hu.
authors.forename: Erzsebet
authors.initials: E
authors.lastname: Paszti-Gere
authors.forename: Reka Fanni
authors.initials: RF
authors.lastname: Barna
authors.forename: Csaba
authors.initials: C
authors.lastname: Kovago
authors.forename: Ipoly
authors.initials: I
authors.lastname: Szauder
authors.forename: Gabriella
authors.initials: G
authors.lastname: Ujhelyi
authors.forename: Csaba
authors.initials: C
authors.lastname: Jakab
authors.forename: Nóra
authors.initials: N
authors.lastname: Meggyesházi
authors.forename: Andras
authors.initials: A
authors.lastname: Szekacs
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D005839:Gentamicins / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012697:Serine Endopeptidases / Q000032:analysis / Q000378:metabolism*; D057057:Serine Proteases / Q000032:analysis / Q000378:metabolism; D013552:Swine
chemical_list: D005839:Gentamicins; D017382:Reactive Oxygen Species; D057057:Serine Proteases; D012697:Serine Endopeptidases; C421305:TMPRSS2 protein, human
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