title: Preparation of crude rough microsomes from dog pancreas.
abstract: This protocol describes how to prepare rough microsomes from dog pancreas. These microsomes can be used to analyze mechanisms of protein translocation and membrane insertion.
authors.affiliation: Department of Cell Biology, NYU Medical School, New York, New York 10016.
authors.forename: David D
authors.initials: DD
authors.lastname: Sabatini
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D004285:Dogs; D008861:Microsomes / Q000201:enzymology*; D010179:Pancreas / Q000201:enzymology*; D014176:Protein Biosynthesis