title: MicroRNA-302a inhibits adipogenesis by suppressing peroxisome proliferator-activated receptor γ expression.
abstract: The present study explored the involvement of miR-302a in adipocyte differentiation via interaction with 3'-untranslated region of peroxisome proliferator-activated receptor gamma (PPARγ) mRNA. In differentiating 3T3-L1 adipocytes, expression of miR-302a was negatively correlated with that of the adipogenic gene aP2 and PPARγ. Overexpression of miR-302a inhibited adipogenic differentiation with lipid accumulation, and inversely anti-miR-302a increased the differentiation. In silico analysis revealed a complementary region of miR-302a seed sequence in 3'-UTR of PPARγ mRNA. Luciferase assay showed the direct interaction of miR-302a with PPARγ at the cellular level. The miR-302a inhibition of adipocyte differentiation was reversed by PPARγ overexpression. These findings suggest that miR-302a might be a negative regulator of adipocyte differentiation and that the dysregulation of miR-302a should lead to metabolic disorders.
authors.affiliation: Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea; Research Center for Biomineralization Disorders and Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Byung-Chul
authors.initials: BC
authors.lastname: Jeong
authors.affiliation: Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea; Research Center for Biomineralization Disorders and Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: In-Hong
authors.initials: IH
authors.lastname: Kang
authors.affiliation: Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea; Research Center for Biomineralization Disorders and Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea. Electronic address: jtkoh@chonnam.ac.kr.
authors.forename: Jeong-Tae
authors.initials: JT
authors.lastname: Koh
mesh_terms: D020413:3' Untranslated Regions; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000502:physiology; D050156:Adipogenesis; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015870:Gene Expression; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000502:physiology*; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference
chemical_list: D020413:3' Untranslated Regions; C558265:MIRN302 microRNA, mouse; D035683:MicroRNAs; D047495:PPAR gamma
keywords: Adipocyte; Differentiation; MiR-302a; Peroxisome proliferator-activated receptor gamma