title: Amphipols and photosynthetic light-harvesting pigment-protein complexes.
abstract: The trimeric light-harvesting complexes II (LHCII) of plants and green algae are pigment-protein complexes involved in light harvesting and photoprotection. Different conformational states have been proposed to be responsible for their different functions. At present, detergent-solubilized LHCII is used as a model for the "light-harvesting conformation", whereas the "quenched conformation" is mimicked by LHCII aggregates. However, none of these conditions seem to perfectly reproduce the properties of LHCII in vivo. In addition, several monomeric LHC complexes are not fully stable in detergent. There is thus a need to find conditions that allow analyzing LHCs in vitro in stable and, hopefully, more native-like conformations. Here, we report a study of LHCII, the major antenna complex of plants, in complex with amphipols. We have trapped trimeric LHCII and monomeric Lhcb1 with either polyanionic or non-ionic amphipols and studied the effect of these polymers on the properties of the complexes. We show that, as compared to detergent solutions, amphipols have a stabilizing effect on LHCII. We also show that the average fluorescence lifetime of LHCII trapped in an anionic amphipol is ~30% shorter than in α-dodecylmaltoside, due to the presence of a conformation with 230-ps lifetime that is not present in detergent solutions.
authors.affiliation: Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands, milenaopacic@gmail.com.
authors.forename: Milena
authors.initials: M
authors.lastname: Opačić
authors.forename: Grégory
authors.initials: G
authors.lastname: Durand
authors.forename: Michael
authors.initials: M
authors.lastname: Bosco
authors.forename: Ange
authors.initials: A
authors.lastname: Polidori
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Popot
mesh_terms: D000818:Animals; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000648:ultrastructure*; D011108:Polymers / Q000737:chemistry*; D011437:Propylamines / Q000737:chemistry*; D011487:Protein Conformation; D012995:Solubility; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D045332:Photosystem II Protein Complex; D011108:Polymers; D011437:Propylamines; D012996:Solutions; D013501:Surface-Active Agents; C499017:amphipol A8-35
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