title: MicroRNA-100 promotes migration and invasion through mammalian target of rapamycin in esophageal squamous cell carcinoma.
abstract: Esophageal squamous cell carcinoma (ESCC) is the predominant histologic subtype of esophageal cancer and is characterized by a high mortality rate and geographic differences in incidence. microRNAs (miRNAs) are small, non-coding RNAs that play important roles in the regulation of genes associated with cancer development and progression. In the present study, we demonstrated that microRNA-100 (miR‑100) demonstrated markedly lower expression in the ESCC tissues as validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Furthermore, we found that the downregulation of miR-100 was significantly correlated with the status of lymph node metastasis in the 34 ESCC patients. Next, we investigated the role and mechanism of miR-100 in ESCC cells and found that miR-100 modulated the migration and invasion but not the apoptosis and proliferation of ESCC cells in vitro. We further demonstrated that miR-100 directly targeted the mTOR 3'UTR and repressed the expression of mTOR, a tumor-related gene. Similarly, miR-100 has been reported as a tumor suppressor by controlling cell migration and invasion, as it can target mTOR genes. These results provide insight into the potential mechanisms of miR-100 in the pathogenesis of ESCC.
authors.affiliation: Department of Laboratory Medicine, The 82nd Hospital of the People's Liberation Army, Huaian 223001, P.R. China.
authors.forename: Naijian
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, The 82nd Hospital of the People's Liberation Army, Huaian 223001, P.R. China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Fu
authors.affiliation: Department of Laboratory Medicine, The 82nd Hospital of the People's Liberation Army, Huaian 223001, P.R. China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of General Surgery, The 82nd Hospital of the People's Liberation Army, Huaian 223001, P.R. China.
authors.forename: Youhong
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Laboratory Medicine, The 82nd Hospital of the People's Liberation Army, Huaian 223001, P.R. China.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, The 82nd Hospital of the People's Liberation Army, Huaian 223001, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, The 82nd Hospital of the People's Liberation Army, Huaian 223001, P.R. China.
authors.forename: Yiqi
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, The 82nd Hospital of the People's Liberation Army, Huaian 223001, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jiao
authors.affiliation: Department of Laboratory Medicine, The 82nd Hospital of the People's Liberation Army, Huaian 223001, P.R. China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D019073:HT29 Cells; D056945:Hep G2 Cells; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D058570:TOR Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C544266:MIRN100 microRNA, human; D035683:MicroRNAs; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases