title: Effect of lentinan on membrane-bound protein expression in splenic lymphocytes under chronic low-dose radiation.
abstract: We investigated the protective effects of lentinan against damages to chronic and low-dose radiation (CL-radiation) by using mouse models. The mice were randomized divided into four groups: normal control mice (Ctr), mice exposed to radiation (Rad), irradiated mice treated with low-dose lentinan (0.1mg/(kg.d), RL), and irradiated mice treated with high-dose lentinan (0.5mg/(kg.d), RH). All the mice were injected intraperitoneally once a day at a dose of 0.5mL (Ctr and Rad with normal sodium while RL and RH with lentinan). The success of radiation models was confirmed by HE stain and cell morphology by a transmission electron microscope (TEM). On the basis of radiation models, we investigated the expression of proteins on the membrane of splenic cells through MALDI-TOF-MS/MS. The results demonstrated that both RT-radiation and lentinan affected the expression of membrane proteins, but lentinan protected the splenic cells and tissue from the injuries caused by CL-radiation. Therefore, we speculated that CL-radiation mainly damages the genetic materials and membrane-bound proteins, while lentinan protects membrane-bound proteins by regulating signal transduction and the appearance of the cells.
authors.affiliation: Department of Pharmacy, No. 401 Hospital of Chinese People's Liberation Army, Qingdao 266071, China.
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, No. 401 Hospital of Chinese People's Liberation Army, Qingdao 266071, China.
authors.forename: Shou-Dong
authors.initials: SD
authors.lastname: Ma
authors.affiliation: Department of Pharmacy, No. 401 Hospital of Chinese People's Liberation Army, Qingdao 266071, China.
authors.forename: Qing-Jie
authors.initials: QJ
authors.lastname: Fu
authors.affiliation: Department of Pharmacy, No. 401 Hospital of Chinese People's Liberation Army, Qingdao 266071, China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Zhao
authors.affiliation: Radiation Oncology Department, No. 401 Hospital of Chinese People's Liberation Army, Qingdao 266071, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Radiation Oncology Department, No. 401 Hospital of Chinese People's Liberation Army, Qingdao 266071, China.
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, No. 401 Hospital of Chinese People's Liberation Army, Qingdao 266071, China. Electronic address: LMC401Y@163.com.
authors.forename: Ming-Chun
authors.initials: MC
authors.lastname: Li
mesh_terms: D000818:Animals; D007912:Lentinan / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D014916:Whole-Body Irradiation
chemical_list: D008565:Membrane Proteins; D011837:Radiation-Protective Agents; D007912:Lentinan
keywords: Lentinan; Lymphocytes; Radioprotection