title: High-throughput Method of One-Step DNA Isolation for PCR Diagnostics of Mycobacterium tuberculosis.
abstract: The efficiency of one-step and multi-step protocols of DNA isolation from lysed sputum samples containing the Mycobacterium tuberculosis complex has been compared. DNA was isolated using spin-cartridges containing a special silica-based sorbent modified with fluoroplast and polyaniline, or using an automated isolation system. One-step isolation using the obtained sorbent has been shown to ensure a significantly lower DNA loss and higher sensitivity in the PCR detection of Mycobacterium tuberculosis as compared to a system based on sorption and desorption of nucleic acids during the isolation.
authors.affiliation: M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, GSP-7, Ul. Miklukho-Maklaya, 16/10, Moscow, Russia, 117997.
authors.forename: D V
authors.initials: DV
authors.lastname: Kapustin
authors.affiliation: M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, GSP-7, Ul. Miklukho-Maklaya, 16/10, Moscow, Russia, 117997.
authors.forename: A I
authors.initials: AI
authors.lastname: Prostyakova
authors.affiliation: ZAO SINTOL, Ul. Timiryazevskaya, 42, Moscow, Russia, 127550 ; Russian National Research Institute of Agricultural Biotechnology of the Russian Academy of Agricultural Sciences, Ul. Timiryazevskaya, 42, Moscow, Russia, 127550.
authors.forename: Ya I
authors.initials: YI
authors.lastname: Alexeev
authors.affiliation: ZAO SINTOL, Ul. Timiryazevskaya, 42, Moscow, Russia, 127550 ; Russian National Research Institute of Agricultural Biotechnology of the Russian Academy of Agricultural Sciences, Ul. Timiryazevskaya, 42, Moscow, Russia, 127550.
authors.forename: D A
authors.initials: DA
authors.lastname: Varlamov
authors.affiliation: M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, GSP-7, Ul. Miklukho-Maklaya, 16/10, Moscow, Russia, 117997.
authors.forename: V P
authors.initials: VP
authors.lastname: Zubov
authors.affiliation: M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, GSP-7, Ul. Miklukho-Maklaya, 16/10, Moscow, Russia, 117997.
authors.forename: S K
authors.initials: SK
authors.lastname: Zavriev
keywords: DNA isolation; Mycobacterium tuberculosis complex; PCR diagnostics; composite sorbents; fluoropolymers; polyaniline
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