title: Genetic Association of CHRNB3 and CHRNA6 Gene Polymorphisms with Nicotine Dependence Syndrome Scale in Korean Population.
abstract: OBJECTIVE
  Cholinergic nicotinic receptor (CHRN) gene family has been known to mediate the highly additive effects of nicotine in the body, and implicated nicotine dependence (ND) and related phenotypes. Previous studies have found that CHRNA6-CHRNB3 cluster polymorphisms were significantly associated with the risk of ND and various tobacco behaviors. The aim of study was to evaluate the genetic association of CHRNB3 and CHRNA6 polymorphisms with the risk of ND based on the Fagerstrom Test for Nicotine Dependence (FTND) score and five subscales of nicotine dependence syndrome scale (NDSS) in Korean population.
  
  
  METHODS
  Six SNPs in CHRNA6-CHRNB3 cluster were analyzed in 576 Korean subjects. Association analysis using logistic models and regression analysis with NDSS were performed.
  
  
  RESULTS
  There was no association in the case-control analysis, whereas all six SNPs were significantly associated with drive factor among NDSS in subgroup based on the FTND score. CHRNB3 rs4954 and CHRNA6 rs16891604 showed significant associations with NDSSF1 (drive) in dominant models among moderate to severe ND among smokers after correction (p(corr) =0.02 and 0.001, respectively), whereas other four SNPs showed significant associations among mild ND after correction (p(corr) =0.03-0.02 in dominant model).
  
  
  CONCLUSIONS
  This study showed that the genetic influence of CHRNB3-CHRNA6 cluster polymorphisms are found in a ND endophenotype (drive) using NDSS subscales, rather than the risk of ND in Korean population. Our findings might be the first report for the association of CHRNB3-CHRNA6 cluster with ND-related phenotypes in Korean and might offer an approach to elucidating the molecular mechanisms of ND and ND-related phenotypes.
authors.affiliation: Department of Psychiatry, The Catholic University of Korea College of Medicine, Seoul, Republic of Korea.
authors.forename: Wang-Youn
authors.initials: WY
authors.lastname: Won
authors.affiliation: Department of Genetic Epidemiology, SNP Genetics, Inc., Seoul, Republic of Korea.
authors.forename: ByungLae
authors.initials: B
authors.lastname: Park
authors.affiliation: Department of Addiction Rehabilitation with Social Welfare, Eulji University, Seongnam, Republic of Korea.
authors.forename: Sam-Wook
authors.initials: SW
authors.lastname: Choi
authors.affiliation: Department of Genetic Epidemiology, SNP Genetics, Inc., Seoul, Republic of Korea.
authors.forename: LyoungHyo
authors.initials: L
authors.lastname: Kim
authors.affiliation: Department of Psychiatry, The Catholic University of Korea College of Medicine, Seoul, Republic of Korea.
authors.forename: Min
authors.initials: M
authors.lastname: Kwon
authors.affiliation: Department of Psychiatry, The Catholic University of Korea College of Medicine, Seoul, Republic of Korea.
authors.forename: Jae-Hwa
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Psychiatry, The Catholic University of Korea College of Medicine, Seoul, Republic of Korea.
authors.forename: Chang-Uk
authors.initials: CU
authors.lastname: Lee
authors.affiliation: Department of Genetic Epidemiology, SNP Genetics, Inc., Seoul, Republic of Korea. ; Department of Life Science, Sogang University, Seoul, Republic of Korea.
authors.forename: Hyoung Doo
authors.initials: HD
authors.lastname: Shin
authors.affiliation: Department of Psychiatry, The Catholic University of Korea College of Medicine, Seoul, Republic of Korea.
authors.forename: Dai-Jin
authors.initials: DJ
authors.lastname: Kim
keywords: CHRNA6; CHRNB3; NDSS; Nicotine dependence; SNP
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