title: An improved method for the electrotransformation of lactic acid bacteria: A comparative survey.
abstract: An efficient method for genetic transformation of lactic acid bacteria (LAB) by electroporation is presented in this work. A comparative survey with other electrotransformation methods already published showed that the method proposed here yields the higher electrotransformation efficiency in the 12 LAB strains tested, which could make the method applicable to other LAB species or genera.
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de La Coruña km 7.5, 28040 Madrid, Spain. Electronic address: landete.josem@inia.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Landete
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de La Coruña km 7.5, 28040 Madrid, Spain.
authors.forename: Juan L
authors.initials: JL
authors.lastname: Arqués
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de La Coruña km 7.5, 28040 Madrid, Spain.
authors.forename: Angela
authors.initials: A
authors.lastname: Peirotén
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de La Coruña km 7.5, 28040 Madrid, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Langa
authors.affiliation: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de La Coruña km 7.5, 28040 Madrid, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Medina
mesh_terms: D018274:Electroporation / Q000379:methods*; D005827:Genetics, Microbial / Q000379:methods*; D056546:Lactobacillales / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D014169:Transformation, Bacterial
keywords: Lactic acid bacteria; Lactobacillus reuteri; Transformation; Voltage