title: Flap endonuclease of bacteriophage T7: Possible roles in RNA primer removal, recombination and host DNA breakdown.
abstract: Gene 6 protein of bacteriophage T7 has 5'-3'-exonuclease activity specific for duplex DNA. We have found that gene 6 protein also has flap endonuclease activity. The flap endonuclease activity is considerably weaker than the exonuclease activity. Unlike the human homolog of gene 6 protein, the flap endonuclease activity of gene 6 protein is dependent on the length of the 5'-flap. This dependency of activity on the length of the 5'-flap may result from the structured helical gateway region of gene 6 protein which differs from that of human flap endonuclease 1. The flap endonuclease activity provides a mechanism by which RNA-terminated Okazaki fragments, displaced by the lagging strand DNA polymerase, are processed. 3'-extensions generated during degradation of duplex DNA by the exonuclease activity of gene 6 protein are inhibitory to further degradation of the 5'-terminus by the exonuclease activity of gene 6 protein. The single-stranded DNA binding protein of T7 overcomes this inhibition.
authors.affiliation: The Department of Biological Chemistry and Molecular Pharmacology; Harvard Medical School; Boston, MA USA.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Mitsunobu
authors.affiliation: The Department of Biological Chemistry and Molecular Pharmacology; Harvard Medical School; Boston, MA USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.affiliation: The Department of Biological Chemistry and Molecular Pharmacology; Harvard Medical School; Boston, MA USA.
authors.forename: Seung-Joo
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: The Department of Biological Chemistry and Molecular Pharmacology; Harvard Medical School; Boston, MA USA.
authors.forename: Stanley
authors.initials: S
authors.lastname: Tabor
authors.affiliation: The Department of Biological Chemistry and Molecular Pharmacology; Harvard Medical School; Boston, MA USA.
authors.forename: Charles C
authors.initials: CC
authors.lastname: Richardson
keywords: DNA replication; Okazaki fragment; bacteriophage T7; flap endonuclease; gene 6 protein
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