title: Expression of bacteriocin divercin AS7 inEscherichia coliand its functional analysis.
abstract: Bacteriocins are small peptides with antimicrobial activity, that are produced by bacteria. Four classes of bacteriocins produced by lactic acid bacteria have been defined. Class IIa bacteriocins are promising candidates for industrial applications due to their high biological activity and their physicochemical properties. Divercin AS7 is a class IIa bacteriocin produced byCarnobacterium divergensAS7. It shows antibacterial activity against pathogens and food spoilage flora includingListeriaspp. Little is known about the impact of class IIa bacteriocins upon eukaryotic cells. The safe use of bacteriocins as food biopreservatives requires the absence of cytotoxicity to human cells. To analyze the impact of divercin AS7 on human enterocytes, we expressed the recombinant divercin AS7 in theEscherichia coliBL21DE3pLys strain and conducted in vitro studies to evaluate the safety of recombinant divercin AS7. No cytotoxic effect on differentiated monolayer Caco-2 cells and no apoptotic appearance were observed when recombinant divercin AS7 was used at a concentration of 2 μg ml-1. In our study, divercin AS7 also did not interfere with differentiated Caco-2 cells monolayer integrity. The obtained results suggest that divercin AS7 is a promising peptide for the food industry.
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego Street No. 48, 60-627 Poznań, Poland.
authors.forename: Agnieszka K
authors.initials: AK
authors.lastname: Olejnik-Schmidt
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego Street No. 48, 60-627 Poznań, Poland.
authors.forename: Marcin T
authors.initials: MT
authors.lastname: Schmidt
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego Street No. 48, 60-627 Poznań, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sip
authors.affiliation: Department of Food Quality Management, Poznan University of Life Sciences, Wojska Polskiego Street No. 31, 60-624 Poznań, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Szablewski
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego Street No. 48, 60-627 Poznań, Poland.
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Grajek
keywords: Bacteriocins; Biopreservatives; Divercin AS7; Human enterocytes
references.citation: J Bacteriol. 2004 Jul;186(13):4276-84
references.pmid: 15205430
references.citation: J Food Prot. 2003 May;66(5):847-53
references.pmid: 12747695
references.citation: Biochem Pharmacol. 1990 Nov 15;40(10):2353-62
references.pmid: 2244936
references.citation: Lett Appl Microbiol. 2000 Jan;30(1):42-6
references.pmid: 10728559
references.citation: Proteomics. 2010 Jun;10(11):2175-90
references.pmid: 20354993
references.citation: Antonie Van Leeuwenhoek. 1996 Oct;70(2-4):113-28
references.pmid: 8879403
references.citation: Appl Microbiol Biotechnol. 2007 Dec;77(3):525-31
references.pmid: 17882416
references.citation: Microbiology (Reading). 1998 Oct;144 ( Pt 10):2837-2844
references.pmid: 9802025
references.citation: Int J Food Microbiol. 1998 Jun 30;42(1-2):63-9
references.pmid: 9706799
references.citation: J Appl Microbiol. 2008 Jan;104(1):1-13
references.pmid: 18171378
references.citation: Lett Appl Microbiol. 2003;36(5):288-92
references.pmid: 12680940
references.citation: Anal Biochem. 1987 Nov 1;166(2):368-79
references.pmid: 2449095
references.citation: Appl Environ Microbiol. 2005 Nov;71(11):7528-30
references.pmid: 16269795
references.citation: J Appl Microbiol. 2005;98(3):676-83
references.pmid: 15715871
references.citation: Br Poult Sci. 2011 Aug;52(4):492-9
references.pmid: 21919577
references.citation: Methods Mol Biol. 2004;268:225-33
references.pmid: 15156034
references.citation: Food Microbiol. 2010 Oct;27(7):869-79
references.pmid: 20688228
references.citation: FEMS Microbiol Rev. 1993 Sep;12(1-3):39-85
references.pmid: 8398217
references.citation: Biochem Pharmacol. 2006 Apr 28;71(9):1289-98
references.pmid: 16530733
references.citation: Int J Food Microbiol. 1995 Dec;28(2):169-85
references.pmid: 8750665
references.citation: J Appl Microbiol. 2004;96(5):931-7
references.pmid: 15078508