title: Arabidopsis SNAREs SYP61 and SYP121 coordinate the trafficking of plasma membrane aquaporin PIP2;7 to modulate the cell membrane water permeability.
abstract: Plant plasma membrane intrinsic proteins (PIPs) are aquaporins that facilitate the passive movement of water and small neutral solutes through biological membranes. Here, we report that post-Golgi trafficking of PIP2;7 in Arabidopsis thaliana involves specific interactions with two syntaxin proteins, namely, the Qc-SNARE SYP61 and the Qa-SNARE SYP121, that the proper delivery of PIP2;7 to the plasma membrane depends on the activity of the two SNAREs, and that the SNAREs colocalize and physically interact. These findings are indicative of an important role for SYP61 and SYP121, possibly forming a SNARE complex. Our data support a model in which direct interactions between specific SNARE proteins and PIP aquaporins modulate their post-Golgi trafficking and thus contribute to the fine-tuning of the water permeability of the plasma membrane.
authors.affiliation: Institut des Sciences de la Vie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
authors.forename: Charles
authors.initials: C
authors.lastname: Hachez
authors.affiliation: Institut des Sciences de la Vie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Timothée
authors.initials: T
authors.lastname: Laloux
authors.affiliation: Institut des Sciences de la Vie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Hagen
authors.initials: H
authors.lastname: Reinhardt
authors.affiliation: Institut des Sciences de la Vie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Damien
authors.initials: D
authors.lastname: Cavez
authors.affiliation: Institut des Sciences de la Vie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Hervé
authors.initials: H
authors.lastname: Degand
authors.affiliation: Zentrum für Molekularbiologie der Pflanzen, Developmental Genetics, University of Tuebingen, D-72076 Tuebingen, Germany.
authors.forename: Christopher
authors.initials: C
authors.lastname: Grefen
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
authors.forename: Riet
authors.initials: R
authors.lastname: De Rycke
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.affiliation: Laboratory of Plant Physiology and Biophysics, Institute of Molecular, Cell, and Systems Biology, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Blatt
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Russinova
authors.affiliation: Institut des Sciences de la Vie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium chaumont@uclouvain.be.
authors.forename: François
authors.initials: F
authors.lastname: Chaumont
mesh_terms: D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D017930:Genes, Reporter; D006056:Golgi Apparatus / Q000378:metabolism; D016254:Mutagenesis, Insertional; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000502:physiology; D054046:Plant Stomata / Q000166:cytology / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D021381:Protein Transport; D040901:Proteomics; D050765:Qa-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D050600:SNARE Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000502:physiology; D014867:Water / Q000378:metabolism
chemical_list: D020346:Aquaporins; D029681:Arabidopsis Proteins; C479425:PEN1 protein, Arabidopsis; D050765:Qa-SNARE Proteins; D011993:Recombinant Fusion Proteins; D050600:SNARE Proteins; C472135:SYP61 protein, Arabidopsis; C491680:plasma membrane intrinsic protein 2 Arabidopsis; D014867:Water
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