title: Short communication: Antimicrobial susceptibility profiling and genotyping of Staphylococcus aureus isolates from bovine mastitis in Poland.
abstract: Staphylococcus aureus is the predominant causative agent of bovine mastitis, a disease that remains a major economic burden for the dairy industry worldwide. In this study, the antimicrobial resistance patterns and the genetic composition of 80 S. aureus mastitis isolates collected from 14 dairy farms in Eastern Poland were determined. Of the 10 antimicrobial agents evaluated, only testing for penicillin G produced drug resistance. As 41% of the S. aureus isolates were penicillin resistant, this drug along with other β-lactamase-sensitive β-lactams, should rather not be considered for the treatment of bovine mastitis caused by S. aureus. Upon genotyping, with a triplex PCR method, a total of 11 distinct PCR types were produced. The population structure of S. aureus isolates was highly clonal, with 1 predominant genotype circulating on each farm. The observed similarities in the genotype composition of S. aureus populations from geographically distant farms underscore the significance of interfarm transmission of S. aureus in Poland. This, in turn, argues for the establishment of a nationwide surveillance program for bovine mastitis due to this pathogen.
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland. Electronic address: t.jagielski@biol.uw.edu.pl.
authors.forename: T
authors.initials: T
authors.lastname: Jagielski
authors.affiliation: National Chamber of Medical Laboratory Specialists, 03-428 Warsaw, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Puacz
authors.affiliation: Department of Animal and Environmental Hygiene, University of Life Sciences, 20-950 Lublin, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Lisowski
authors.affiliation: Department of Plant Molecular Biology, Institute of Plant Experimental Biology and Biotechnology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Siedlecki
authors.affiliation: Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Cracow, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Dudziak
authors.affiliation: Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Cracow, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Międzobrodzki
authors.affiliation: Department of Animal and Environmental Hygiene, University of Life Sciences, 20-950 Lublin, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Krukowski
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000890:Anti-Infective Agents / Q000627:therapeutic use; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005260:Female; D005838:Genotype; D008414:Mastitis, Bovine / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D008826:Microbial Sensitivity Tests / Q000662:veterinary*; D010403:Penicillin Resistance / Q000235:genetics; D011044:Poland; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000662:veterinary; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D001618:beta-Lactamases; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; D001618:beta-Lactamases
keywords: Staphylococcus aureus; bovine mastitis; drug resistance; genotyping