title: A time-resolved fluorescence immunoassay for the ultrasensitive determination of diethylstilbestrol based on the double-codified gold nanoparticles.
abstract: An ultrasensitive and selective method is presented for the determination of diethylstilbestrol (DES) using time-resolved fluorescence immunoassay (TRFIA) based on double-codified gold nanoparticles (DC-AuNPs). In this system, the DC-AuNPs, that are gold nanoparticles (AuNPs) modified with anti-DES antibody and SH-dsDNA-biotin, was regarded as signal amplifier. A competitive immunoreaction was performed on polystyrene microtitration plates, where the DES compete with the immobilized DES-ovalbumin on polystyrene microtitration plates to bind to anti-DES antibodies on DC-AuNPs, and the europium(III)-labeled streptavidin was added to link to the SH-dsDNA-biotin as a tracer. Fluorescence signal was amplified via the AuNPs and the biotin-streptavidin double amplification systems. Under the optimized condition, DES can be quantified by TRFIA. The linear range and the limit of detection of DES were 1.0×10(-6)-10ngmL(-1) and 0.4fgmL(-1), respectively. This method was applied to determine DES in beef sample, with the recoveries ranging from 88% to 105%.
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.
authors.forename: Longjun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.
authors.forename: Yuanfu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Science, Liaocheng University, Liaocheng 252059, China.
authors.forename: Guofu
authors.initials: G
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China. Electronic address: shuhaowang@sohu.com.
authors.forename: Shuhao
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry / Q000276:immunology; D001710:Biotin / Q000737:chemistry; D002417:Cattle; D004247:DNA / Q000737:chemistry; D004054:Diethylstilbestrol / Q000302:isolation & purification* / Q000633:toxicity; D005453:Fluorescence; D005504:Food Analysis / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D008461:Meat Products / Q000506:poisoning; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D001710:Biotin; D004054:Diethylstilbestrol; D006046:Gold; D004247:DNA
keywords: Amplification technique; Diethylstilbestrol; Double-codified gold nanoparticles; Time-resolved fluorescence immunoassay