title: [Baicalein induces HeLa cell growth inhibition by down-regulation of matrix metalloproteinases and activating extracellular signal-regulated kinase].
abstract: OBJECTIVE
  To observe the changes of mRNA and protein expressions of extracellular signal-regulated kinase 1/2 (ERK1/2) and matrix metalloproteinase 2 (MMP-2), MMP-9 in cervical cancer cells after stimulated by baicalein at different concentrations.
  
  
  METHODS
  HeLa cells cultured in vitro were exposed to baicalein (0, 100 and 200 μmol/L) for 24 hours. The activity of MMP-2 and MMP-9 was measured by gelatin zymography, mRNA expressions of ERK1/2, MMP-2, MMP-9 were determined by reverse transcription PCR (RT-PCR) and protein expressions of ERK1/2, MMP-2, MMP-9 were detected by Western blotting.
  
  
  RESULTS
  Compared with the control group, baicalein significantly decreased the activity of MMP-2 and MMP-9 in a concentration-dependent manner (P<0.05). The mRNA and protein expressions of ERK1/2, p-ERK1/2, MMP-2, MMP-9 also significantly decreased (P<0.05).
  
  
  CONCLUSIONS
  Baicalein can activate ERK1/2 signaling pathway and decreasing the activity of MMP-2 and MMP-9.
authors.affiliation: Luzhou Medical College, Maternal and Child Care Service Centre of Longmatan District, Luzhou 646000, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Yu
authors.affiliation: Maternal and Child Care Service Centre of Luzhou, Luzhou 646000, China.
authors.forename: Kelian
authors.initials: K
authors.lastname: Lu
authors.affiliation: Research Center for Drug and Functional Food, Luzhou 646000, China.
authors.forename: Jiyi
authors.initials: J
authors.lastname: Xia
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital, Luzhou Medical College, Luzhou 646000, China.
authors.forename: Xiguang
authors.initials: X
authors.lastname: Mao
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D044950:Flavanones / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000975:Antioxidants; D044950:Flavanones; C006680:baicalein; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9