title: Bifunctional DNA architectonics: three-way junctions with sticky perylene bisimide caps and a central metal lock.
abstract: A new type of a bifunctional DNA architecture based on a three way junction is developed that combines the structural motif of sticky perylene bisimide caps with a tris-bipyridyl metal ion lock in the center part. A clear stabilizing effect was observed in the presence of Fe(3+), Ni(2+) and Zn(2+) by the formation of corresponding bipyridyl complexes in the branching part of the DNA three way junctions. The dimerization of the 5'-terminally attached perylene diimides (PDI) chromophores by hydrophobic interactions can be followed by significant changes in the UV/Vis absorption and steady-state fluorescence. The PDI-mediated DNA assembly occurs at temperatures below the melting temperature and is not influenced by the metal-ion bipyridyl locks in the central part. The corresponding AFM images revealed the formation of higher-ordered structures as the result of DNA assemblies mediated by the PDI interactions.
authors.affiliation: Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131 Karlsruhe (Germany), Fax: (+49)-721-608-44825.
authors.forename: Claudia
authors.initials: C
authors.lastname: Stubinitzky
authors.forename: Andrea
authors.initials: A
authors.lastname: Bijeljanin
authors.forename: Linda
authors.initials: L
authors.lastname: Antusch
authors.forename: Daniel
authors.initials: D
authors.lastname: Ebeling
authors.forename: Hendrik
authors.initials: H
authors.lastname: Hölscher
authors.forename: Hans-Achim
authors.initials: HA
authors.lastname: Wagenknecht
mesh_terms: D002413:Cations, Divalent / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D019281:Dimerization; D005290:Ferric Compounds / Q000737:chemistry; D007094:Imides / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry*; D015394:Molecular Structure; D009532:Nickel / Q000737:chemistry; D009690:Nucleic Acid Conformation; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000737:chemistry
chemical_list: D002413:Cations, Divalent; D005290:Ferric Compounds; D007094:Imides; D019216:Metals, Heavy; C510697:perylene bisimide; D010569:Perylene; D009532:Nickel; D004247:DNA; D015032:Zinc
keywords: DNA architectonics; bipyridines; exciton; fluorescence; oligonucleotides