title: Exogenous methyl jasmonate treatment increases glucosinolate biosynthesis and quinone reductase activity in kale leaf tissue.
abstract: Methyl jasmonate (MeJA) spray treatments were applied to the kale varieties 'Dwarf Blue Curled Vates' and 'Red Winter' in replicated field plantings in 2010 and 2011 to investigate alteration of glucosinolate (GS) composition in harvested leaf tissue. Aqueous solutions of 250 µM MeJA were sprayed to saturation on aerial plant tissues four days prior to harvest at commercial maturity. The MeJA treatment significantly increased gluconasturtiin (56%), glucobrassicin (98%), and neoglucobrassicin (150%) concentrations in the apical leaf tissue of these genotypes over two seasons. Induction of quinone reductase (QR) activity, a biomarker for anti-carcinogenesis, was significantly increased by the extracts from the leaf tissue of these two cultivars. Extracts of apical leaf tissues had greater MeJA mediated increases in phenolics, glucosinolate concentrations, GS hydrolysis products, and QR activity than extracts from basal leaf tissue samples. The concentration of the hydrolysis product of glucoraphanin, sulforphane was significantly increased in apical leaf tissue of the cultivar 'Red Winter' in both 2010 and 2011. There was interaction between exogenous MeJA treatment and environmental conditions to induce endogenous JA. Correlation analysis revealed that indole-3-carbanol (I3C) generated from the hydrolysis of glucobrassicin significantly correlated with QR activity (r = 0.800, P<0.001). Concentrations required to double the specific QR activity (CD values) of I3C was calculated at 230 µM, which is considerably weaker at induction than other isothiocyanates like sulforphane. To confirm relationships between GS hydrolysis products and QR activity, a range of concentrations of MeJA sprays were applied to kale leaf tissues of both cultivars in 2011. Correlation analysis of these results indicated that sulforaphane, NI3C, neoascorbigen, I3C, and diindolylmethane were all significantly correlated with QR activity. Thus, increased QR activity may be due to combined increases in phenolics (quercetin and kaempferol) and GS hydrolysis product concentrations rather than by individual products alone.
authors.affiliation: Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.
authors.forename: Kang-Mo
authors.initials: KM
authors.lastname: Ku
authors.affiliation: Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.
authors.forename: Elizabeth H
authors.initials: EH
authors.lastname: Jeffery
authors.affiliation: Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.
authors.forename: John A
authors.initials: JA
authors.lastname: Juvik
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D053898:Biosynthetic Pathways / Q000187:drug effects; D001937:Brassica / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005961:Glucosinolates / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D054883:Oxylipins / Q000494:pharmacology*; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005961:Glucosinolates; D054883:Oxylipins; D010636:Phenols; D010936:Plant Extracts; D010937:Plant Growth Regulators; C072239:methyl jasmonate; D016660:NAD(P)H Dehydrogenase (Quinone); D006026:Glycoside Hydrolases; C022739:thioglucosidase
references.citation: Phytochemistry. 2006 Feb;67(4):395-401
references.pmid: 16403544
references.citation: Phytochem Anal. 2011 Mar-Apr;22(2):144-52
references.pmid: 21259374
references.citation: Free Radic Biol Med. 2004 Aug 1;37(3):287-303
references.pmid: 15223063
references.citation: Anal Biochem. 2005 Aug 1;343(1):93-9
references.pmid: 15963940
references.citation: J Natl Cancer Inst. 1999 Apr 7;91(7):605-13
references.pmid: 10203279
references.citation: Food Chem Toxicol. 2003 Apr;41(4):455-62
references.pmid: 12615118
references.citation: Carcinogenesis. 2000 Nov;21(11):1983-8
references.pmid: 11062158
references.citation: Nat Toxins. 1995;3(4):299-304; discussion 317
references.pmid: 7582633
references.citation: Toxicol Sci. 2005 Jan;83(1):126-35
references.pmid: 15483186
references.citation: Oecologia. 2011 Jun;166(2):421-31
references.pmid: 21140168
references.citation: Biosci Biotechnol Biochem. 2009 Sep;73(9):1962-70
references.pmid: 19734678
references.citation: J Sci Food Agric. 2014 Aug;94(10):2090-6
references.pmid: 24338840
references.citation: Carcinogenesis. 1996 Nov;17(11):2385-7
references.pmid: 8968052
references.citation: Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2399-403
references.pmid: 1549603
references.citation: Anal Biochem. 1988 Mar;169(2):328-36
references.pmid: 3382006
references.citation: J Nat Prod. 2006 Mar;69(3):460-3
references.pmid: 16562858
references.citation: Annu Rev Entomol. 2009;54:57-83
references.pmid: 18811249
references.citation: Proc Natl Acad Sci U S A. 1995 May 9;92(10):4114-9
references.pmid: 11607536
references.citation: Cancer Lett. 1997 Dec 9;120(2):213-6
references.pmid: 9461039
references.citation: J Agric Food Chem. 2007 Feb 7;55(3):955-62
references.pmid: 17263499
references.citation: Nutr Rev. 1998 Apr;56(4 Pt 1):127-30
references.pmid: 9584498
references.citation: J Agric Food Chem. 2013 Oct 9;61(40):9623-31
references.pmid: 24032372
references.citation: Methods Enzymol. 2004;382:380-414
references.pmid: 15047113
references.citation: Chem Biol Interact. 1998 Apr 24;111-112:377-88
references.pmid: 9679568
references.citation: Plant Foods Hum Nutr. 2013 Jun;68(2):113-7
references.pmid: 23640295
references.citation: Drug News Perspect. 2005 Sep;18(7):445-51
references.pmid: 16362084
references.citation: In Vivo. 2008 Jul-Aug;22(4):441-5
references.pmid: 18712169
references.citation: Clin Exp Pharmacol Physiol. 2000 Mar;27(3):152-9
references.pmid: 10744340
references.citation: Science. 2009 Jan 2;323(5910):101-6
references.pmid: 19095900
references.citation: Food Chem. 2014;152:190-6
references.pmid: 24444925
references.citation: Chromatographia. 2011 Jun;73(Suppl 1):137-142
references.pmid: 21765536
references.citation: Molecules. 2010 Oct 21;15(10):7313-52
references.pmid: 20966876
references.citation: PLoS One. 2013 Oct 16;8(10):e77127
references.pmid: 24146962