title: Antidiabetic effect of Lactobacillus casei CCFM0412 on mice with type 2 diabetes induced by a high-fat diet and streptozotocin.
abstract: OBJECTIVE
  The aim of this study was to evaluate the antidiabetic effects of Lactobacillus casei CCFM0412 on mice with type 2 diabetes induced by a high-fat diet and streptozotocin.
  
  
  METHODS
  Thirty-two male C57 BL/6 J mice were assigned to four groups in this study. Type 2 diabetes was induced by feeding of a high-fat diet and injection of streptozotocin. L. casei CCFM0412 was administered to mice at a dose of 10(9) cfu/d per mouse for 12 wk. Body weight, fasting and postprandial 2-h blood glucose, oral glucose tolerance, glycosylated hemoglobin, insulin, and glycogen in liver were measured. Endotoxin, tumor necrosis factor-α, and interleukin-10 levels were determined. Lipid metabolic parameters including triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were also measured. The activities of glutathione peroxides, reactive oxygen species, and superoxide dismutase, and the levels of glutathione and malondialdehyde in the liver also were determined. Pancreas injury was evaluated by histologic analysis.
  
  
  RESULTS
  At 13 wk, L. casei CCFM0412 significantly decreased fasting and postprandial 2-h blood glucose, glycosylated hemoglobin, endotoxin, tumor necrosis factor-α, triglycerides, total cholesterol, low-density lipoprotein cholesterol, reactive oxygen species, and malondialdehyde levels compared with the control group (P < 0.05). The values for insulin, interleukin-10, high-density lipoprotein cholesterol, glutathione peroxides, superoxide dismutase, glutathione, and glycogen were significantly increased at 13 wk (P < 0.05). Islets of Langerhans in the L. casei CCFM0412 group were substantially protected from destruction compared with those in the control group.
  
  
  CONCLUSIONS
  L. casei CCFM0412 significantly improved glucose intolerance, dyslipidemia, immune-regulatory properties, and oxidative stress in mice with type 2 diabetes induced by a high-fat diet and streptozotocin. The results provide a sound rationale for future clinical trials of oral administration of L. casei CCFM0412 for the primary prevention of type 2 diabetes.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P.R. China; School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, P.R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi 2, P.R. China.
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P.R. China; College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dang
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi 2, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P.R. China.
authors.forename: Fengwei
authors.initials: F
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P.R. China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi 2, P.R. China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi 2, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P.R. China. Electronic address: Chenwei66@jiangnan.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy* / Q000209:etiology; D059305:Diet, High-Fat; D050171:Dyslipidemias / Q000188:drug therapy / Q000209:etiology; D004731:Endotoxins / Q000097:blood; D018149:Glucose Intolerance / Q000097:blood / Q000188:drug therapy / Q000209:etiology; D006442:Glycated Hemoglobin / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000187:drug effects; D007780:Lacticaseibacillus casei; D008055:Lipids / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D013311:Streptozocin; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D004731:Endotoxins; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D007328:Insulin; D008055:Lipids; D008315:Malondialdehyde; D013311:Streptozocin; D014409:Tumor Necrosis Factor-alpha
keywords: Antidiabetic effect; Blood glucose; Lactobacillus casei CCFM0412; Pioglitazone; Type 2 diabetes