title: Interaction of the nematophagous fungus Pochonia chlamydosporia and Parascaris equorum eggs in different culture media.
abstract: Research involving the use of nematophagous fungi in the biological control of parasites of interest to veterinarians has occurred over recent years, with promising results. This article reports the infection of Parascaris equorum eggs by the fungus Pochonia chlamydosporia (isolates VC1 and VC4). Six groups were formed for each isolate, with six different culture media: 2% water-agar (2% WA); agar-chitin (AC); YPSSA (yeast extract, K2HPO4, MgSO4 ·7H2O, soluble starch); AELA extract (starch + water + agar); 2% corn-meal-agar (2% CMA); and 2% potato dextrose-agar (2% PDA). A total of 1000 eggs of P. equorum were transferred to each plate containing isolates grown for a period of 7 days (treatment group). Also, 1000 eggs were added to each plate without fungus (controlgroup). The plates were kept in an environmental chamber at 25 °C in the dark for 21 days. After, we analyzed the effects on ovicidal activity: effect 1 (accession shell); effect 2 (penetration hyphae); and effect 3 (destruction of the eggs). No differences were observed in the destruction of eggs between the two isolates. The decreasing effectiveness of the different culture media was: PDA (38.9%); CMA (38.3%); WA (36.7%); YPSSA (36.45%); and AC (32.5%). The highest percentage egg destruction was observed when the strains were grown in culture medium AELA (44.9%); this was the best medium.
authors.affiliation: Department of Veterinary, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: Lorendane Millena
authors.initials: LM
authors.lastname: de Carvalho
authors.forename: Fabio Ribeiro
authors.initials: FR
authors.lastname: Braga
authors.forename: Rafael Reis
authors.initials: RR
authors.lastname: Domingues
authors.forename: Juliana Milani
authors.initials: JM
authors.lastname: Araujo
authors.forename: Rosane Teixeira
authors.initials: RT
authors.lastname: Lelis
authors.forename: Alessandra Teixeira
authors.initials: AT
authors.lastname: de Paula
authors.forename: Wendeo Ferreira
authors.initials: WF
authors.lastname: da Silveira
authors.forename: Jackson Victor
authors.initials: JV
authors.lastname: de Araújo
mesh_terms: D000818:Animals; D000898:Antibiosis; D001199:Ascaridoidea / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D003624:Darkness; D054884:Host-Pathogen Interactions; D006999:Hypocreales / Q000254:growth & development* / Q000502:physiology; D016019:Survival Analysis; D013696:Temperature; D013995:Time; D015053:Zygote / Q000382:microbiology*
chemical_list: D003470:Culture Media
keywords: Biological control; Culture medium; Equine Ascarididae; Nematophagous fungi; Ovicidal fungi