title: Apocynum Tablet Protects against Cardiac Hypertrophy via Inhibiting AKT and ERK1/2 Phosphorylation after Pressure Overload.
abstract: Background. Cardiac hypertrophy occurs in many cardiovascular diseases. Apocynum tablet (AT), a traditional Chinese medicine, has been widely used in China to treat patients with hypertension. However, the underlying molecular mechanisms of AT on the hypertension-induced cardiac hypertrophy remain elusive. The current study evaluated the effect and mechanisms of AT on cardiac hypertrophy. Methods. We created a mouse model of cardiac hypertrophy by inducing pressure overload with surgery of transverse aortic constriction (TAC) and then explored the effect of AT on the development of cardiac hypertrophy using 46 mice in 4 study groups (combinations of AT and TAC). In addition, we evaluated the signaling pathway of phosphorylation of ERK1/2, AKT, and protein expression of GATA4 in the cardioprotective effects of AT using Western blot. Results. AT inhibited the phosphorylation of Thr202/Tyr204 sites of ERK1/2, Ser473 site of AKT, and protein expression of GATA4 and significantly inhibited cardiac hypertrophy and cardiac fibrosis at 2 weeks after TAC surgery (P < 0.05). Conclusions. We experimentally demonstrated that AT inhibits cardiac hypertrophy via suppressing phosphorylation of ERK1/2 and AKT.
authors.affiliation: Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, 101 Dade Road, Yuexiu District, Guangzhou 510120, China.
authors.forename: Jianyong
authors.identifier: 0000-0002-2290-740X
authors.initials: J
authors.lastname: Qi
authors.affiliation: Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, 101 Dade Road, Yuexiu District, Guangzhou 510120, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Second Clinical Medical School, Yangzhou University, Yangzhou 225001, China.
authors.forename: Kaizheng
authors.initials: K
authors.lastname: Gong
authors.affiliation: Animal Laboratory, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China.
authors.forename: Juan
authors.identifier: 0000-0003-2706-906X
authors.initials: J
authors.lastname: Yu
authors.affiliation: Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, 101 Dade Road, Yuexiu District, Guangzhou 510120, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, 101 Dade Road, Yuexiu District, Guangzhou 510120, China.
authors.forename: Liheng
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Oral and Maxillary Surgery, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Northeast Ohio Medical University, Rootstown, OH 44272, USA.
authors.forename: Jiashin
authors.identifier: 0000-0002-8003-6235
authors.initials: J
authors.lastname: Wu
authors.affiliation: Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, 101 Dade Road, Yuexiu District, Guangzhou 510120, China.
authors.forename: Minzhou
authors.identifier: 0000-0002-5657-7891
authors.initials: M
authors.lastname: Zhang
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