title: Photoprotection of Buddleja cordata extract against UVB-induced skin damage in SKH-1 hairless mice.
abstract: BACKGROUND
  In recent years, there has been considerable interest in using botanical agents to prevent skin damage resulting from solar UV-irradiation. Buddleja cordata is a plant that is known as "tepozan". Some people in Mexico use the leaves of this plant to treat tumours, abscesses, sores and burns. The purpose of this study is to investigate the photoprotective properties of Buddleja cordata methanolic extract (BCME) against UVB-induced skin damage in SKH-1 hairless mice at the macroscopic and histological levels.
  
  
  METHODS
  BCME was characterised to determine its spectroscopic, chromatographic and antioxidant (DPPH, superoxide and hydroxyl radicals) properties. To conduct the photoprotection studies, BCME was applied topically to the skin of SKH-1 mice before acute exposure to UVB for 10 minutes. The murine skin samples were used for macroscopic and histological studies to assess tissue damage. Penetration of active components of BCME into stratum corneum on the dorsal area of mice was investigated in vivo by the tape stripping method. Moreover, genotoxicity of BCME was evaluated in a Vicia faba cell root micronucleus model.
  
  
  RESULTS
  BCME displayed absorbance over the entire UVB spectrum, and its principal components included verbascoside and linarin. BCME exhibited antioxidant activity and significantly scavenged hydroxyl radicals. BCME reduced erythema, sunburn cell production, vessel congestion and epidermal thickening of UVB irradiated mouse skin. BCME penetrate the skin of mice. BCME did not exhibit genotoxic activity in the micronucleus test.
  
  
  CONCLUSIONS
  The topical administration of BCME protected against acute UVB-induced damage in mouse SKH-1 skin, and our results suggest that BCME may potentially prevent photodamage.
authors.affiliation: Laboratorio de Fitoquímica, Unidad de Biología, Tecnología y Prototipos (UBIPRO), Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Av, de los Barrios No, 1, Los Reyes Iztacala, Tlalnepantla, Edo, de México C,P, 54090, México. tuncomaclovio2000@yahoo.com.mx.
authors.forename: José Guillermo
authors.initials: JG
authors.lastname: Avila Acevedo
authors.forename: Adriana Montserrat
authors.initials: AM
authors.lastname: Espinosa González
authors.forename: Diana Matamoros
authors.initials: DM
authors.lastname: De Maria y Campos
authors.forename: José del Carmen
authors.initials: Jdel C
authors.lastname: Benitez Flores
authors.forename: Tzasna
authors.initials: T
authors.lastname: Hernández Delgado
authors.forename: Saul
authors.initials: S
authors.lastname: Flores Maya
authors.forename: Jorge
authors.initials: J
authors.lastname: Campos Contreras
authors.forename: José Luis
authors.initials: JL
authors.lastname: Muñoz López
authors.forename: Ana María
authors.initials: AM
authors.lastname: García Bores
mesh_terms: D000287:Administration, Topical; D000818:Animals; D032861:Buddleja / Q000737:chemistry*; D004890:Erythema / Q000517:prevention & control; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011897:Random Allocation; D012867:Skin / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D013471:Sunburn / Q000517:prevention & control; D013473:Sunscreening Agents / Q000737:chemistry / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D010636:Phenols; D010936:Plant Extracts; D013473:Sunscreening Agents
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