title: Basal and β-adrenergic cardiomyocytes contractility dysfunction induced by dietary protein restriction is associated with downregulation of SERCA2a expression and disturbance of endoplasmic reticulum Ca2+ regulation in rats.
abstract: The mechanisms responsible for the cardiac dysfunction associated with dietary protein restriction (PR) are poorly understood. Thus, this study was designed to evaluate the effects of PR on calcium kinetics, basal and β-adrenergic contractility in murine ventricular cardiomyocytes.
  
  
  
  After breastfeeding male Fisher rats were distributed into a control group (CG, n = 20) and a protein-restricted group (PRG, n = 20), receiving isocaloric diets for 35 days containing 15% and 6% protein, respectively. Biometric and hemodynamic variables were measured. After euthanasia left ventricles (LV) were collected for histopathological evaluation, SERCA2a expression, cardiomyocytes contractility and Ca(2+)sparks analysis.
  
  
  
  PRG animals showed reduced general growth, increased heart rate and arterial pressure. These animals presented extracellular matrix expansion and disorganization, cardiomyocytes hypotrophy, reduced amplitudes of shortening and maximum velocity of contraction and relaxation at baseline and after β-adrenergic stimulation. Reduced SERCA2a expression as well as higher frequency and lower amplitude of Ca(2+)sparks were observed in PRG cardiomyocytes.
  
  
  
  The observations reveal that protein restriction induces marked myocardial morphofunctional damage. The pathological changes of cardiomyocyte mechanics suggest the potential involvement of the β-adrenergic system, which is possibly associated with changes in SERCA2a expression and disturbances in Ca(2+) intracellular kinetics.
authors.affiliation: Department of Basic Sciences, Federal University of Jequitinhonha and Mucuri Vale, Diamantina, MG, Brazil.
authors.forename: Arlete R
authors.initials: AR
authors.lastname: Penitente
authors.forename: Rômulo D
authors.initials: RD
authors.lastname: Novaes
authors.forename: Marcelo E
authors.initials: ME
authors.lastname: Silva
authors.forename: Márcia F
authors.initials: MF
authors.lastname: Silva
authors.forename: Judson F
authors.initials: JF
authors.lastname: Quintão-Júnior
authors.forename: Silvia
authors.initials: S
authors.lastname: Guatimosim
authors.forename: Jader S
authors.initials: JS
authors.lastname: Cruz
authors.forename: Deoclécio A
authors.initials: DA
authors.lastname: Chianca
authors.forename: Antônio J
authors.initials: AJ
authors.lastname: Natali
authors.forename: Clóvis A
authors.initials: CA
authors.lastname: Neves
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004044:Dietary Proteins / Q000008:administration & dosage*; D015536:Down-Regulation; D008297:Male; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*
chemical_list: D004044:Dietary Proteins; D011943:Receptors, Adrenergic, beta; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium