title: High prevalence of extended-spectrum β-lactamase, plasmid-mediated AmpC, and carbapenemase genes in pet food.
abstract: We evaluated the pet food contained in 30 packages as a potential origin of extended-spectrum cephalosporin-resistant Gram-negative organisms and β-lactamase genes (bla). Live bacteria were not detected by selective culture. However, PCR investigations on food DNA extracts indicated that samples harbored the blaCTX-M-15 (53.3%), blaCMY-4 (20%), and blaVEB-4-like (6.7%) genes. Particularly worrisome was the presence of blaOXA-48-like carbapenemases (13.3%). The original pet food ingredients and/or the production processes were highly contaminated with bacteria carrying clinically relevant acquired bla genes.
authors.affiliation: Institute for Infectious Diseases, Faculty of Medicine, University of Bern, Bern, Switzerland Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
authors.forename: Salome N
authors.initials: SN
authors.lastname: Seiffert
authors.affiliation: Institute for Infectious Diseases, Faculty of Medicine, University of Bern, Bern, Switzerland Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Carattoli
authors.affiliation: Institute for Infectious Diseases, Faculty of Medicine, University of Bern, Bern, Switzerland.
authors.forename: Regula
authors.initials: R
authors.lastname: Tinguely
authors.affiliation: Institute for Infectious Diseases, Faculty of Medicine, University of Bern, Bern, Switzerland.
authors.forename: Agnese
authors.initials: A
authors.lastname: Lupo
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors.forename: Vincent
authors.initials: V
authors.lastname: Perreten
authors.affiliation: Institute for Infectious Diseases, Faculty of Medicine, University of Bern, Bern, Switzerland andrea.endimiani@ifik.unibe.ch.
authors.forename: Andrea
authors.initials: A
authors.lastname: Endimiani
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
references.citation: Antimicrob Agents Chemother. 2001 Dec;45(12):3647-50
references.pmid: 11709361
references.citation: J Antimicrob Chemother. 2010 Mar;65(3):490-5
references.pmid: 20071363
references.citation: J Antimicrob Chemother. 2010 Dec;65(12):2518-29
references.pmid: 20935300
references.citation: Emerg Infect Dis. 2011 Oct;17(10):1791-8
references.pmid: 22000347
references.citation: J Antimicrob Chemother. 2013 Dec;68(12):2802-8
references.pmid: 23833179
references.citation: Vet Microbiol. 2014 Jul 16;171(3-4):290-7
references.pmid: 24629777
references.citation: Int J Environ Res Public Health. 2013 Jun 28;10(7):2643-69
references.pmid: 23812024
references.citation: Antimicrob Agents Chemother. 2013 Jan;57(1):674-6
references.pmid: 23114767
references.citation: FEMS Microbiol Lett. 2004 May 15;234(2):201-7
references.pmid: 15135523
references.citation: Clin Microbiol Rev. 2009 Jan;22(1):161-82, Table of Contents
references.pmid: 19136439
references.citation: Curr Pharm Des. 2013;19(2):257-63
references.pmid: 22934977
references.citation: Diagn Microbiol Infect Dis. 2012 May;73(1):31-8
references.pmid: 22578936
references.citation: Clin Microbiol Infect. 2010 Jan;16(1):33-8
references.pmid: 19681957
references.citation: Antimicrob Agents Chemother. 2014;58(4):2446-9
references.pmid: 24468781
references.citation: Vector Borne Zoonotic Dis. 2013 Feb;13(2):98-102
references.pmid: 23289399
references.citation: Antimicrob Agents Chemother. 2004 Dec;48(12):4895-7
references.pmid: 15561876
references.citation: Drug Resist Updat. 2013 Feb-Apr;16(1-2):22-45
references.pmid: 23395305
references.citation: J Clin Microbiol. 2011 Sep;49(9):3411-4
references.pmid: 21775537
references.citation: J Microbiol Methods. 2005 Dec;63(3):219-28
references.pmid: 15935499
references.citation: J Antimicrob Chemother. 2013 Oct;68(10):2249-54
references.pmid: 23759671
references.citation: J Antimicrob Chemother. 2012 Jul;67(7):1597-606
references.pmid: 22499996
references.citation: PLoS One. 2014 Mar 04;9(3):e90564
references.pmid: 24595207
references.citation: Clin Microbiol Infect. 2011 Jun;17(6):873-80
references.pmid: 21463397
references.citation: Int J Food Microbiol. 2013 Nov 1;167(3):359-62
references.pmid: 24184615
references.citation: Antimicrob Agents Chemother. 2013 Dec;57(12):6406-8
references.pmid: 24080656
references.citation: Microb Drug Resist. 2014 Oct;20(5):485-94
references.pmid: 24773305
references.citation: Antimicrob Agents Chemother. 2012 Jan;56(1):559-62
references.pmid: 22083465