title: MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro.
abstract: Elite controllers maintain HIV-1 viral loads below the limit of detection. The mechanisms responsible for this phenomenon are poorly understood. As microRNAs (miRNAs) are regulators of gene expression and some of them modulate HIV infection, we have studied the miRNA profile in plasma from HIV elite controllers and chronically infected individuals and compared against healthy donors. Several miRNAs correlate with CD4+ T cell count or with the known time of infection. No significant differences were observed between elite controllers and healthy donors; however, 16 miRNAs were different in the plasma of chronic infected versus healthy donors. In addition, levels of hsa-miR-29b-3p, hsa-miR-33a-5p and hsa-miR-146a-5p were higher in plasma from elite controllers than chronic infected and hsa-miR-29b-3p and hsa-miR-33a-5p overexpression significantly reduced the viral production in MT2 and primary T CD4+ cells. Therefore, levels of circulating miRNAs might be of diagnostic and/or prognostic value for HIV infection, and hsa-miR-29b-3p and miR-33a-5p may contribute to the design of new anti-HIV drugs.
authors.affiliation: Department of Biotechnology, CD laboratory on Biotechnology of Skin Aging, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Rita
authors.initials: R
authors.lastname: Reynoso
authors.affiliation: 1] Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Universidad de Buenos Aires/CONICET, Buenos Aires, Argentina [2] CONICET, Argentina [3] J.A. Fernández Hospital, Infectious Diseases Unit, Buenos Aires, Argentina.
authors.forename: Natalia
authors.initials: N
authors.lastname: Laufer
authors.affiliation: 1] Department of Biotechnology, CD laboratory on Biotechnology of Skin Aging, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 18, A-1190 Vienna, Austria [2] TAmiRNA GmbH, Muthgasse 11, A-1190 Vienna, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hackl
authors.affiliation: Evercyte GmbH, Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Susanna
authors.initials: S
authors.lastname: Skalicky
authors.affiliation: Department of Biotechnology, CD laboratory on Biotechnology of Skin Aging, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Rossella
authors.initials: R
authors.lastname: Monteforte
authors.affiliation: 1] Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Universidad de Buenos Aires/CONICET, Buenos Aires, Argentina [2] CONICET, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Turk
authors.affiliation: 1] Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Universidad de Buenos Aires/CONICET, Buenos Aires, Argentina [2] CONICET, Argentina.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Carobene
authors.affiliation: 1] Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Universidad de Buenos Aires/CONICET, Buenos Aires, Argentina [2] CONICET, Argentina.
authors.forename: Jorge
authors.initials: J
authors.lastname: Quarleri
authors.affiliation: 1] J.A. Fernández Hospital, Infectious Diseases Unit, Buenos Aires, Argentina [2] Huesped Foundation, Buenos Aires, Argentina.
authors.forename: Pedro
authors.initials: P
authors.lastname: Cahn
authors.affiliation: Division of Virology, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Roland
authors.initials: R
authors.lastname: Werner
authors.affiliation: Division of Virology, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Heribert
authors.initials: H
authors.lastname: Stoiber
authors.affiliation: 1] Department of Biotechnology, CD laboratory on Biotechnology of Skin Aging, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 18, A-1190 Vienna, Austria [2] Evercyte GmbH, Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Regina
authors.initials: R
authors.lastname: Grillari-Voglauer
authors.affiliation: 1] Department of Biotechnology, CD laboratory on Biotechnology of Skin Aging, BOKU - University of Natural Resources and Life Sciences Vienna, Muthgasse 18, A-1190 Vienna, Austria [2] Evercyte GmbH, Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
mesh_terms: D000328:Adult; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D016022:Case-Control Studies; D002478:Cells, Cultured; D060467:Disease Resistance; D005260:Female; D015658:HIV Infections / Q000097:blood* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D059467:Transcriptome; D014779:Virus Replication; D055815:Young Adult
chemical_list: C542542:MIRN146 microRNA, human; C532045:MIRN29a microRNA, human; C550416:MIRN33a microRNA, human; D035683:MicroRNAs
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