title: Ultrastructural and immunohistochemical study of the effect of sodium alendronate in the progression of experimental periodontitis in rats.
abstract: The aim of the present research was to investigate the ultrastructural aspects and the immunoexpression of receptor activator of NFκB ligand (RANKL) and osteoprotegerin (OPG) on experimental periodontal disease of alendronate (ALN)-treated rats. Male Wistar rats received daily injections of 2.5 mg/kg body weight of ALN during 7 days previously and 7, 14, and 21 days after the insertion of a 4.0 silk suture into the gingival sulcus around the right upper second molar. Specimens were fixed in 0.1% glutaraldehyde + 4% formaldehyde under microwave irradiation, decalcified in 4.13% EDTA and paraffin embedded for TRAP histochemistry and immunohistochemistry for RANKL and OPG, or embedded in Spurr epoxy resin for TEM analysis. ALN reduced the activity of osteoclasts and significantly decreased the resorption of the alveolar crest. In the control group the alveolar crest appeared resorbed by TRAP-positive osteoclasts, which presented ultrastructural features of activated cells. The immunoexpression of RANKL was not inhibited by the drug; however, the expression of OPG was increased in the treated animals. The alveolar crest of ALN-treated specimens at 21 days showed signs of osteonecrosis, like empty osteocyte lacunae, the exposed bone regions and bacterial infection. The results showed that ALN treatment in individuals with periodontal disease represents a risk of osteonecrosis because of the reduced activity of osteoclasts resultant of the increased immunoexpression of OPG.
authors.affiliation: Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
authors.forename: Mariana M
authors.initials: MM
authors.lastname: Moreira
authors.forename: Vivian
authors.initials: V
authors.lastname: Bradaschia-Correa
authors.forename: Natasha D M
authors.initials: ND
authors.lastname: Marques
authors.forename: Lorraine Braga
authors.initials: LB
authors.lastname: Ferreira
authors.forename: Victor E
authors.initials: VE
authors.lastname: Arana-Chavez
mesh_terms: D019386:Alendronate / Q000494:pharmacology*; D000539:Alveolar Process / Q000187:drug effects / Q000648:ultrastructure; D000818:Animals; D003771:Dental Papilla / Q000187:drug effects / Q000648:ultrastructure; D018450:Disease Progression; D005881:Gingiva / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D053244:Osteoprotegerin / Q000378:metabolism; D010518:Periodontitis / Q000139:chemically induced* / Q000473:pathology; D053245:RANK Ligand / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; C505688:TNFSF11 protein, human; D019386:Alendronate
keywords: alendronate; immunohistochemistry; osteoprotegerin; periodontitis; receptor activator of NFκB ligand; transmission electron microscopy