title: Expression of genes for AhR and Nrf2 signal pathways in the retina of OXYS rats during the development of retinopathy and melatonin-induced changes in this process.
abstract: Modulation of oxidative stress is one of the experimental approaches to the therapy of age-related macular degeneration. Melatonin holds much promise in this respect. It was hypothesized that the efficiency of melatonin in age-related macular degeneration is associated with its ability to modulate gene expression for the AhR and Nrf2 signal pathways. Experiments were performed on premature aging OXYS rats, which serve as a reliable model of age-related macular degeneration in humans. We studied the effect of melatonin on gene mRNA for the AhR and Nrf2 signal pathways. Melatonin was shown to decrease the level of mRNA for AhR-dependent genes of CYP1A2 and CYP1B1 cytochromes in the retina, but had no effect on the content of mRNA for Nrf2-dependent genes in OXYS rats.
authors.affiliation: Research Institute of Molecular Biology and Biophysics, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia, perepech@soramn.ru.
authors.forename: M L
authors.initials: ML
authors.lastname: Perepechaeva
authors.forename: N A
authors.initials: NA
authors.lastname: Stefanova
authors.forename: A Yu
authors.initials: AY
authors.lastname: Grishanova
mesh_terms: D000375:Aging / Q000235:genetics* / Q000473:pathology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D019388:Cytochrome P-450 CYP1A2; D065633:Cytochrome P-450 CYP1B1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D003580:Cytochromes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008268:Macular Degeneration / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D051267:NF-E2-Related Factor 2 / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress; D011371:Progeria / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D051381:Rats; D055647:Rats, Transgenic; D017208:Rats, Wistar; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics* / Q000378:metabolism; D012160:Retina / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000975:Antioxidants; D051792:Basic Helix-Loop-Helix Proteins; D019388:Cytochrome P-450 CYP1A2; D065633:Cytochrome P-450 CYP1B1; D003580:Cytochromes; D008550:Melatonin; D051267:NF-E2-Related Factor 2; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; C000595703:Ahr protein, rat; C495637:Nfe2l2 protein, rat; C017170:Cyp1a2 protein, rat; C585499:Cyp1b1 protein, rat