title: EROD activity and cytochrome P4501A induction in liver and gills of Senegal sole Solea senegalensis from a polluted Huelva estuary (SW Spain).
abstract: EROD activity and induction cytochrome P4501A in liver and gills of Senegal sole, Solea senegalensis, from a heavy metal and PAH polluted estuary, was studied. Liver and gill CYP1A catalytic activity was assessed at the enzyme activity level-measured as 7-ethoxyresorufin-O-deethylase and cellular localization of CYP1A in the liver was studied using immunohistochemistry. Liver EROD was correlated with phenanthrene-type metabolites in liver and copper concentrations in water. Strong CYP1A occurrence was observed in acinar pancreatic cells, pancreatic duct epithelium and vascular system endothelium and negative/rare induction were observed in hepatocytes and sinusoidal endothelium. In gills, EROD activity showed a significant correlation with different fractions of heavy metals in sediment but no correlation was observed between EROD activity and PAHs. Strongly positive CYP1A associated staining of the vascular system endothelia and primary filament cells and a moderate staining of pillar cells in gills were observed. The results substantiated the utility of EROD activity and CYP1A induction measurement as biomarkers for use by aquatic toxicologists and indicate that catalytic assays and immunohistochemical assays appear to be sensitive to different kinds of pollutants being the use of both methods recommended for monitoring programs.
authors.affiliation: Department of Biology, Environmental and Marine Science Faculty, University of Cadiz, Puerto Real, Cadiz, Spain. Electronic address: milagrosa.oliva@uca.es.
authors.forename: Milagrosa
authors.initials: M
authors.lastname: Oliva
authors.affiliation: CIMAR-LA/CIIMAR & ICBAS - Centro Interdisciplinar de Investigação Marinha e Ambiental & Instituto de Ciências Biomédicas de Abel Salazar, Laboratório de Ecotoxicologia, University of Porto, Portugal.
authors.forename: Carlos
authors.initials: C
authors.lastname: Gravato
authors.affiliation: CIMAR-LA/CIIMAR & ICBAS - Centro Interdisciplinar de Investigação Marinha e Ambiental & Instituto de Ciências Biomédicas de Abel Salazar, Laboratório de Ecotoxicologia, University of Porto, Portugal.
authors.forename: Lucia
authors.initials: L
authors.lastname: Guilhermino
authors.affiliation: Department of Analytical Chemistry, Sciences Faculty, University of Cadiz, Puerto Real, Cadiz, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Galindo-Riaño
authors.affiliation: Department of Environmental Technologies, Faculty of Marine and Environmental Sciences, University of Cadiz, Puerto Real, Cadiz, Spain.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Perales
mesh_terms: D000818:Animals; D001267:Atlantic Ocean; D015415:Biomarkers / Q000378:metabolism; D003300:Copper / Q000032:analysis / Q000633:toxicity; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism*; D065693:Cytochrome P-450 Enzyme Inducers / Q000032:analysis / Q000633:toxicity; D004781:Environmental Exposure / Q000009:adverse effects*; D004784:Environmental Monitoring / Q000379:methods; D004790:Enzyme Induction / Q000187:drug effects; D063366:Estuaries; D029941:Fish Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D005412:Flatfishes / Q000254:growth & development / Q000378:metabolism*; D005880:Gills / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009928:Organ Specificity; D010616:Phenanthrenes / Q000032:analysis / Q000633:toxicity; D021381:Protein Transport / Q000187:drug effects; D012623:Seawater / Q000737:chemistry; D012987:Soil / Q000737:chemistry; D013030:Spain; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D065693:Cytochrome P-450 Enzyme Inducers; D029941:Fish Proteins; D010616:Phenanthrenes; D012987:Soil; D014874:Water Pollutants, Chemical; D003300:Copper; D019363:Cytochrome P-450 CYP1A1
keywords: CYP1A; EROD; Heavy metals; PAHs; Sole