title: Serum neutralization assay can efficiently replace plaque reduction neutralization test for detection and quantitation of West Nile virus antibodies in human and animal serum samples.
abstract: A serum neutralization assay (SN) was compared with the official plaque reduction neutralization test for the quantitation of West Nile virus antibodies. A total of 1,348 samples from equid sera and 38 from human sera were tested by these two methods. Statistically significant differences were not observed, thus supporting the use of SN for routine purposes.
authors.affiliation: OIE Reference Laboratory for West Nile Disease, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, G. Caporale, Teramo, Italy.
authors.forename: Annapia
authors.initials: A
authors.lastname: Di Gennaro
authors.affiliation: OIE Reference Laboratory for West Nile Disease, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, G. Caporale, Teramo, Italy a.lorusso@izs.it.
authors.forename: Alessio
authors.initials: A
authors.lastname: Lorusso
authors.affiliation: OIE Reference Laboratory for West Nile Disease, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, G. Caporale, Teramo, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Casaccia
authors.affiliation: OIE Reference Laboratory for West Nile Disease, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, G. Caporale, Teramo, Italy.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Conte
authors.affiliation: OIE Reference Laboratory for West Nile Disease, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, G. Caporale, Teramo, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Monaco
authors.affiliation: OIE Reference Laboratory for West Nile Disease, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise, G. Caporale, Teramo, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Savini
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D006736:Horses; D006801:Humans; D009500:Neutralization Tests / Q000379:methods*; D010948:Viral Plaque Assay / Q000379:methods; D014902:West Nile virus / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral
references.citation: Vector Borne Zoonotic Dis. 2014 Jan;14(1):82-4
references.pmid: 24283515
references.citation: J Gen Virol. 1989 Jan;70 ( Pt 1):37-43
references.pmid: 2543738
references.citation: Vet Microbiol. 2012 Aug 17;158(3-4):267-73
references.pmid: 22406344
references.citation: Adv Virus Res. 2003;61:3-65
references.pmid: 14714429
references.citation: Clin Vaccine Immunol. 2009 Jul;16(7):1052-9
references.pmid: 19458204
references.citation: Vet Microbiol. 2011 Aug 5;151(3-4):264-74
references.pmid: 21550731
references.citation: Zoonoses Public Health. 2008 Sep;55(7):361-7
references.pmid: 18667029
references.citation: Open Virol J. 2010 Apr 22;4:29-37
references.pmid: 20517490