title: Selection and molecular characterization of a high tocopherol accumulation rice mutant line induced by gamma irradiation.
abstract: Tocopherols are micronutrients with antioxidant properties. They are synthesized by photosynthetic bacteria and plants, and play important roles in animal and human nutrition. In this study, we isolated a new rice mutant line with elevated tocopherol content (MRXII) from an in vitro mutagenized population induced by gamma irradiation. The mutant exhibited greater seed longevity than the control, indicating a crucial role for tocopherols in maintaining viability during quiescence, and displayed faster seedling growth during the early growth stage. To study the molecular mechanism underlying vitamin E biosynthesis, we examined the expression patterns of seven rice genes encoding vitamin E biosynthetic enzymes. Accumulation levels of the OsVTE2 transcript and OsVTE2 protein in the MRXII mutant were significantly higher than in the control. Sequence analysis revealed that the MRXII mutant harbored a point mutation in the OsVTE2 promoter region, which resulted in the generation of MYB transcription factor-binding cis-element. These results help identify the promoter regions that regulate OsVTE2 transcription, and offer insights into the regulation of tocopherol content.
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong, Jeongeup, Jeonbuk, 580-185, Republic of Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Hwang
authors.forename: Joon-Woo
authors.initials: JW
authors.lastname: Ahn
authors.forename: Soon-Jae
authors.initials: SJ
authors.lastname: Kwon
authors.forename: Jin-Baek
authors.initials: JB
authors.lastname: Kim
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Si-Yong
authors.initials: SY
authors.lastname: Kang
authors.forename: Dong Sub
authors.initials: DS
authors.lastname: Kim
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D053898:Biosynthetic Pathways / Q000235:genetics / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers / Q000235:genetics; D005720:Gamma Rays; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D024505:Tocopherols / Q000493:pharmacokinetics*; D014810:Vitamin E / Q000096:biosynthesis*
chemical_list: D017931:DNA Primers; D014810:Vitamin E; D019883:Alkyl and Aryl Transferases; D024505:Tocopherols
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