title: Different effects of implanting sensory nerve or blood vessel on the vascularization, neurotization, and osteogenesis of tissue-engineered bone in vivo.
abstract: To compare the different effects of implanting sensory nerve tracts or blood vessel on the osteogenesis, vascularization, and neurotization of the tissue-engineered bone in vivo, we constructed the tissue engineered bone and implanted the sensory nerve tracts (group SN), blood vessel (group VB), or nothing (group Blank) to the side channel of the bone graft to repair the femur defect in the rabbit. Better osteogenesis was observed in groups SN and VB than in group Blank, and no significant difference was found between groups SN and VB at 4, 8, and 12 weeks postoperatively. The neuropeptides expression and the number of new blood vessels in the bone tissues were increased at 8 weeks and then decreased at 12 weeks in all groups and were highest in group VB and lowest in group Blank at all three time points. We conclude that implanting either blood vessel or sensory nerve tract into the tissue-engineered bone can significantly enhance both the vascularization and neurotization simultaneously to get a better osteogenesis effect than TEB alone, and the method of implanting blood vessel has a little better effect of vascularization and neurotization but almost the same osteogenesis effect as implanting sensory nerve.
authors.affiliation: Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jun-jun
authors.initials: JJ
authors.lastname: Fan
authors.affiliation: Department of Orthopaedics, Longgang District Central Hospital of Shenzhen, Shenzhen 518116, China.
authors.forename: Tian-wang
authors.initials: TW
authors.lastname: Mu
authors.affiliation: Department of Orthopaedics, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China.
authors.forename: Jun-jun
authors.initials: JJ
authors.lastname: Qin
authors.affiliation: Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Long
authors.initials: L
authors.lastname: Bi
authors.affiliation: Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Guo-xian
authors.initials: GX
authors.lastname: Pei
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D005269:Femur / Q000473:pathology / Q000601:surgery*; D006801:Humans; D018919:Neovascularization, Physiologic; D016067:Nerve Transfer / Q000379:methods; D010012:Osteogenesis / Q000235:genetics*; D011817:Rabbits; D011984:Sensory Receptor Cells / Q000473:pathology; D014656:Vascular Surgical Procedures
references.citation: Bone. 1999 Dec;25(6):623-9
references.pmid: 10593406
references.citation: Biochem Biophys Res Commun. 1988 Mar 30;151(3):1113-21
references.pmid: 2833256
references.citation: Am J Physiol Endocrinol Metab. 2003 Mar;284(3):E627-33
references.pmid: 12556355
references.citation: J Bone Miner Res. 1999 Aug;14(8):1302-9
references.pmid: 10457262
references.citation: Circulation. 1991 Mar;83(3):774-7
references.pmid: 1999028
references.citation: Orthopedics. 2008 Jun;31(6):591-7
references.pmid: 18661882
references.citation: Stem Cells. 2004;22(7):1279-91
references.pmid: 15579646
references.citation: Clin Orthop Relat Res. 1994 Jan;(298):286-95
references.pmid: 8118989
references.citation: Acta Orthop Scand. 1994 Dec;65(6):629-34
references.pmid: 7839850
references.citation: Biomed Mater. 2010 Oct;5(5):055002
references.pmid: 20826910
references.citation: Eur Cell Mater. 2008 May 02;15:100-14
references.pmid: 18454418
references.citation: J Cell Mol Med. 2007 Jan-Feb;11(1):6-20
references.pmid: 17367498
references.citation: Zhonghua Wai Ke Za Zhi. 2008 Aug 15;46(16):1249-52
references.pmid: 19094602
references.citation: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2010 May;24(5):599-605
references.pmid: 20540269
references.citation: Orthopedics. 2007 Nov;30(11):939-42
references.pmid: 18019987
references.citation: Tissue Eng. 2004 Jul-Aug;10(7-8):978-86
references.pmid: 15363155
references.citation: Cytokine. 1999 Mar;11(3):200-7
references.pmid: 10209067
references.citation: N Engl J Med. 2004 Apr 15;350(16):1655-64
references.pmid: 15084698
references.citation: Crit Rev Oral Biol Med. 1998;9(2):224-44
references.pmid: 9603237
references.citation: J Auton Nerv Syst. 1996 Jun 10;59(1-2):27-33
references.pmid: 8816362
references.citation: Bone. 1996 May;18(5):479-86
references.pmid: 8739907
references.citation: Spine (Phila Pa 1976). 1998 Aug 15;23(16):1793-5
references.pmid: 9728380
references.citation: Plast Reconstr Surg. 2006 Apr 15;117(5):1471-9
references.pmid: 16641715
references.citation: Injury. 2007 Sep;38 Suppl 4:S7-12
references.pmid: 18224732
references.citation: Tissue Eng. 2006 Jul;12(7):1721-31
references.pmid: 16889503
references.citation: Endocrinology. 1986 Jul;119(1):58-61
references.pmid: 3487444
references.citation: Tissue Eng. 2007 Jul;13(7):1549-60
references.pmid: 17518756
references.citation: Am J Physiol Lung Cell Mol Physiol. 2003 Jul;285(1):L86-96
references.pmid: 12626334
references.citation: Clin Pharmacol Ther. 2003 Apr;73(4):312-21
references.pmid: 12709721
references.citation: Science. 1986 May 16;232(4752):868-71
references.pmid: 3518059
references.citation: Bone. 1996 Apr;18(4):331-5
references.pmid: 8726390
references.citation: Injury. 2007 Mar;38 Suppl 1:S75-80
references.pmid: 17383488
references.citation: J Clin Invest. 1999 Aug;104(4):399-407
references.pmid: 10449432
references.citation: J Orthop Res. 1997 Jan;15(1):133-40
references.pmid: 9066538
references.citation: J Cell Mol Med. 2006 Jan-Mar;10(1):7-19
references.pmid: 16563218
references.citation: Neuropeptides. 2009 Dec;43(6):457-63
references.pmid: 19748118
references.citation: J Dent Res. 2006 Jun;85(6):488-95
references.pmid: 16723642
references.citation: J Craniofac Genet Dev Biol. 1990;10(3):329-36
references.pmid: 1702095