title: Evaluation of a new recombinant oncolytic vaccinia virus strain GLV-5b451 for feline mammary carcinoma therapy.
abstract: Virotherapy on the basis of oncolytic vaccinia virus (VACV) infection is a promising approach for cancer therapy. In this study we describe the establishment of a new preclinical model of feline mammary carcinoma (FMC) using a recently established cancer cell line, DT09/06. In addition, we evaluated a recombinant vaccinia virus strain, GLV-5b451, expressing the anti-vascular endothelial growth factor (VEGF) single-chain antibody (scAb) GLAF-2 as an oncolytic agent against FMC. Cell culture data demonstrate that GLV-5b451 virus efficiently infected, replicated in and destroyed DT09/06 cancer cells. In the selected xenografts of FMC, a single systemic administration of GLV-5b451 led to significant inhibition of tumor growth in comparison to untreated tumor-bearing mice. Furthermore, tumor-specific virus infection led to overproduction of functional scAb GLAF-2, which caused drastic reduction of intratumoral VEGF levels and inhibition of angiogenesis. In summary, here we have shown, for the first time, that the vaccinia virus strains and especially GLV-5b451 have great potential for effective treatment of FMC in animal model.
authors.affiliation: Department of Biochemistry, University of Wuerzburg, Wuerzburg, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Adelfinger
authors.affiliation: Department of Biochemistry, University of Wuerzburg, Wuerzburg, Germany; Genelux Corporation, San Diego Science Center, San Diego, California, United States of America.
authors.forename: Ivaylo
authors.initials: I
authors.lastname: Gentschev
authors.affiliation: Department of Biochemistry, University of Wuerzburg, Wuerzburg, Germany.
authors.forename: Julio
authors.initials: J
authors.lastname: Grimm de Guibert
authors.affiliation: Department of Biochemistry, University of Wuerzburg, Wuerzburg, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Weibel
authors.affiliation: Department of Biochemistry, University of Wuerzburg, Wuerzburg, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Langbein-Laugwitz
authors.affiliation: Department of Biochemistry, University of Wuerzburg, Wuerzburg, Germany; Genelux GmbH, Bernried, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Härtl
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine, Hannover, Germany; Division of Medicine Clinic III, Hematology, Oncology and Palliative Medicine University of Rostock, Rostock, Germany.
authors.forename: Hugo
authors.initials: H
authors.lastname: Murua Escobar
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine, Hannover, Germany.
authors.forename: Ingo
authors.initials: I
authors.lastname: Nolte
authors.affiliation: Genelux Corporation, San Diego Science Center, San Diego, California, United States of America; Department of Radiation Medicine and Applied Sciences, Rebecca & John Moores Comprehensive Cancer Center, University of California San Diego, La Jolla, California, United States of America.
authors.forename: Nanhai G
authors.initials: NG
authors.lastname: Chen
authors.affiliation: Genelux Corporation, San Diego Science Center, San Diego, California, United States of America.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Aguilar
authors.affiliation: Genelux Corporation, San Diego Science Center, San Diego, California, United States of America; Department of Radiation Medicine and Applied Sciences, Rebecca & John Moores Comprehensive Cancer Center, University of California San Diego, La Jolla, California, United States of America.
authors.forename: Yong A
authors.initials: YA
authors.lastname: Yu
authors.affiliation: Genelux Corporation, San Diego Science Center, San Diego, California, United States of America; Department of Radiation Medicine and Applied Sciences, Rebecca & John Moores Comprehensive Cancer Center, University of California San Diego, La Jolla, California, United States of America.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Genelux Corporation, San Diego Science Center, San Diego, California, United States of America.
authors.forename: Alexa
authors.initials: A
authors.lastname: Frentzen
authors.affiliation: Department of Biochemistry, University of Wuerzburg, Wuerzburg, Germany; Genelux Corporation, San Diego Science Center, San Diego, California, United States of America; Division of Medicine Clinic III, Hematology, Oncology and Palliative Medicine University of Rostock, Rostock, Germany; Department of Radiation Medicine and Applied Sciences, Rebecca & John Moores Comprehensive Cancer Center, University of California San Diego, La Jolla, California, United States of America; Rudolf Virchow Center for Experimental Biomedicine, University of Wuerzburg, Wuerzburg, Germany; Institute for Molecular Infection Biology, University of Wuerzburg, Wuerzburg, Germany.
authors.forename: Aladar A
authors.initials: AA
authors.lastname: Szalay
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics; D002522:Chlorocebus aethiops; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics* / Q000493:pharmacokinetics; D015674:Mammary Neoplasms, Animal / Q000235:genetics* / Q000276:immunology / Q000473:pathology* / Q000628:therapy; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000628:therapy; D050130:Oncolytic Virotherapy; D050504:Oncolytic Viruses / Q000235:genetics*; D057127:Single-Chain Antibodies / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic; D014616:Vaccinia virus / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication; D023041:Xenograft Model Antitumor Assays
chemical_list: D057127:Single-Chain Antibodies; D042461:Vascular Endothelial Growth Factor A
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