title: Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: application to optical biochips.
abstract: A new type of titanate nanotube (TNT) coating is investigated for exploitation in biosensor applications. The TNT layers were prepared from stable but additive-free sols without applying any binding compounds. The simple, fast spin-coating process was carried out at room temperature, and resulted in well-formed films around 10nm thick. The films are highly transparent as expected from their nanostructure and may, therefore, be useful as coatings for surface-sensitive optical biosensors to enhance the specific surface area. In addition, these novel coatings could be applied to medical implant surfaces to control cellular adhesion. Their morphology and structure was characterized by spectroscopic ellipsometry (SE) and atomic force microscopy (AFM), and their chemical state by X-ray photoelectron spectroscopy (XPS). For quantitative surface adhesion studies, the films were prepared on optical waveguides. The coated waveguides were shown to still guide light; thus, their sensing capability remains. Protein adsorption and cell adhesion studies on the titanate nanotube films and on smooth control surfaces revealed that the nanostructured titanate enhanced the adsorption of albumin; furthermore, the coatings considerably enhanced the adhesion of living mammalian cells (human embryonic kidney and preosteoblast).
authors.affiliation: Nanobiosensorics Laboratory, Institute of Technical Physics and Materials Science, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Konkoly Thege M. út 29-33, Budapest H-1121, Hungary; Doctoral School of Molecular- and Nanotechnologies, University of Pannonia, Egyetem u. 10., Veszprém H-8200, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Nador
authors.affiliation: Nanobiosensorics Laboratory, Institute of Technical Physics and Materials Science, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Konkoly Thege M. út 29-33, Budapest H-1121, Hungary.
authors.forename: Norbert
authors.initials: N
authors.lastname: Orgovan
authors.affiliation: Nanobiosensorics Laboratory, Institute of Technical Physics and Materials Science, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Konkoly Thege M. út 29-33, Budapest H-1121, Hungary.
authors.forename: Miklos
authors.initials: M
authors.lastname: Fried
authors.affiliation: Nanobiosensorics Laboratory, Institute of Technical Physics and Materials Science, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Konkoly Thege M. út 29-33, Budapest H-1121, Hungary.
authors.forename: Peter
authors.initials: P
authors.lastname: Petrik
authors.affiliation: Nanobiosensorics Laboratory, Institute of Technical Physics and Materials Science, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Konkoly Thege M. út 29-33, Budapest H-1121, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Sulyok
authors.affiliation: Clore Laboratory, University of Buckingham, Hunter Street, Buckingham MK18 1EG, UK.
authors.forename: Jeremy J
authors.initials: JJ
authors.lastname: Ramsden
authors.affiliation: Department of Biotechnology, Nanophage Therapy Center, Enviroinvest Corporation, Kertváros u. 2., Pécs H-7632, Hungary.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Korosi
authors.affiliation: Nanobiosensorics Laboratory, Institute of Technical Physics and Materials Science, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Konkoly Thege M. út 29-33, Budapest H-1121, Hungary. Electronic address: horvathr@mfa.kfki.hu.
authors.forename: Robert
authors.initials: R
authors.lastname: Horvath
mesh_terms: D000327:Adsorption; D002448:Cell Adhesion; D018625:Microscopy, Atomic Force; D043942:Nanotubes; D056951:Photoelectron Spectroscopy; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D014025:Titanium / Q000737:chemistry*
chemical_list: D011506:Proteins; D014025:Titanium
keywords: Additive-free; Cell adhesion; Implant; Nanostructured layer; Optical biosensor; Osteoblast