title: Somatic cells, stem cells, and induced pluripotent stem cells: how do they now contribute to conservation?
abstract: More than a decade has now passed since the birth of the first endangered species produced from an adult somatic cell reprogrammed by somatic cell nuclear transfer. At that time, advances made in domestic and laboratory animal species provided the necessary foundation for attempting cutting-edge technologies on threatened and endangered species. In addition to nuclear transfer, spermatogonial stem cell transplantation and induction of pluripotent stem cells have also been explored. Although many basic scientific questions have been answered and more than 30 wild species have been investigated, very few successes have been reported. The majority of studies document numerous obstacles that still need to be overcome to produce viable gametes or embryos for healthy offspring production. This chapter provides an overview of somatic cell and stem cell technologies in different taxa (mammals, fishes, birds, reptiles and amphibians) and evaluates the potential and impact of these approaches for animal species conservation.
authors.affiliation: Reproductive Physiology, Toronto Zoo, Toronto, ON, Canada, gmastromonaco@torontozoo.ca.
authors.forename: Gabriela F
authors.initials: GF
authors.lastname: Mastromonaco
authors.forename: L Antonio
authors.initials: LA
authors.lastname: González-Grajales
authors.forename: Melissa
authors.initials: M
authors.lastname: Filice
authors.forename: Pierre
authors.initials: P
authors.lastname: Comizzoli
mesh_terms: D000818:Animals; D003247:Conservation of Natural Resources; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*