title: A rapid and label-free SERS detection method for biomarkers in clinical biofluids.
abstract: A metal carbonyl-functionalized nanostructured substrate can be used in a rapid and simple assay for the detection of A1AT, a potential biomarker for bladder cancer, in clinical urine samples. The assay involves monitoring changes in the carbonyl stretching vibrations of the metal carbonyl via surface-enhanced Raman spectroscopy (SERS). These vibrations lie in the absorption spectral window of 1800-2200 cm(-1), which is free of any spectral interference from biomolecules.
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 138667, Singapore.
authors.forename: Kien Voon
authors.initials: KV
authors.lastname: Kong
authors.forename: Weng Kee
authors.initials: WK
authors.lastname: Leong
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lam
authors.forename: Tianxun
authors.initials: T
authors.lastname: Gong
authors.forename: Douglas
authors.initials: D
authors.lastname: Goh
authors.forename: Weber Kam On
authors.initials: WK
authors.lastname: Lau
authors.forename: Malini
authors.initials: M
authors.lastname: Olivo
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D001826:Body Fluids / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D013059:Spectrum Analysis, Raman / Q000379:methods*; D020349:Surface Plasmon Resonance
chemical_list: D015415:Biomarkers
keywords: A1AT; SERS; bioassays; biomarkers; functionalized substrates; osmium carbonyl clusters; urine