title: Novel lutein loaded lipid nanoparticles on porcine corneal distribution.
abstract: Topical delivery has the advantages including being user friendly and cost effective. Development of topical delivery carriers for lutein is becoming an important issue for the ocular drug delivery. Quantification of the partition coefficient of drug in the ocular tissue is the first step for the evaluation of delivery efficacy. The objectives of this study were to evaluate the effects of lipid nanoparticles and cyclodextrin (CD) on the corneal lutein accumulation and to measure the partition coefficients in the porcine cornea. Lipid nanoparticles combined with 2% HPβCD could enhance lutein accumulation up to 209.2 ± 18 (μg/g) which is 4.9-fold higher than that of the nanoparticles. CD combined nanoparticles have 68% of drug loading efficiency and lower cytotoxicity in the bovine cornea cells. From the confocal images, this improvement is due to the increased partitioning of lutein to the corneal epithelium by CD in the lipid nanoparticles. The novel lipid nanoparticles could not only improve the stability and entrapment efficacy of lutein but also enhance the lutein accumulation and partition in the cornea. Additionally the corneal accumulation of lutein was further enhanced by increasing the lutein payload in the vehicles.
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, 259 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan 33302, Taiwan ; Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, 261 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan 33303, Taiwan.
authors.forename: Chi-Hsien
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, 259 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan 33302, Taiwan.
authors.forename: Hao-Che
authors.initials: HC
authors.lastname: Chiu
authors.affiliation: College of Medicine, Chang Gung University, 259 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan 33302, Taiwan ; Department of Ophthalmology, Chang Gung Memorial Hospital, 5 Fusing Street, Kwei-Shan, Tao-Yuan 33305, Taiwan.
authors.forename: Wei-Chi
authors.initials: WC
authors.lastname: Wu
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, 259 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan 33302, Taiwan.
authors.forename: Soubhagya Laxmi
authors.initials: SL
authors.lastname: Sahoo
authors.affiliation: Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, 261 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan 33303, Taiwan.
authors.forename: Ching-Yun
authors.initials: CY
authors.lastname: Hsu
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