title: Genome-wide screening of pathogenicity islands in Mycobacterium tuberculosis based on the genomic barcode visualization.
abstract: Mycobacterium tuberculosis (M. tuberculosis) is one of the most widely spread human pathogenic bacteria, and it frequently exchanges pathogenesis genes among its strains or with other pathogenic microbes. The purpose of this study was to screen the pathogenicity islands (PAIs) in M. tuberculosis using the genomic barcode visualization technique and to characterize the functions of the detected PAIs. By visually screening the barcode image of the M. tuberculosis chromosomes, three candidate PAIs were detected as MPI-1, MPI-2 and MPI-3, among which MPI-2 and MPI-3 were known to harbor pathogenesis genes, and MPI-1 represents a novel candidate. Based on the functional annotations of Pfam domains and GO categories, both MPI-2 and MPI-3 carry genes encoding PE/PPE family proteins, MPI-2 encodes the type VII secretion system, and MPI-3 encodes genes for mycolic acid synthesis in the cell wall. Some of these genes were already widely used in early diagnosis or treatment of M. tuberculosis. The novel candidate PAI MPI-1 encodes CRISPR-C as family proteins, which are known to be associated with persistent infection of M. tuberculosis. Our data represents a molecular basis and protocol for comprehensive annotating the pathogenic systems of M. tuberculosis, and will also facilitate the development of diagnosis and vaccination techniques of M. tuberculosis.
authors.affiliation: Norman Bethune Medical College of Jilin University, Changchun, 130021, Jilin, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Xie
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Zhou
authors.forename: Guangyu
authors.initials: G
authors.lastname: Xu
authors.forename: Guoqin
authors.initials: G
authors.lastname: Mai
authors.forename: Jie
authors.initials: J
authors.lastname: Hu
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic; D030541:Databases, Genetic; D016680:Genome, Bacterial; D044404:Genomic Islands; D023281:Genomics; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins
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