title: Effect of thickness of HA-coating on microporous silk scaffolds using alternate soaking technology.
abstract: Hydroxyapatite (HA) can be coated on various materials surface and has the function of osteogenicity. Microporous silk scaffold has excellent biocompatibility. In this study, alternate soaking technology was used to coat HA on microporous silk scaffolds. However, the cell proliferation was found to decrease with the increasing thickness (cycles of soaking) of HA-coating. This study aims to determine the best thickness (cycles of soaking) of HA-coating on microporous silk scaffolds. The SEM observation showed that group with one cycle of alternate soaking (1C-HA) has the most optimal porosity like non-HA-modified microporous silk scaffolds. The proliferation of osteoblasts has no significant difference between noncoated HA (N-HA) and 1C-HA groups, which are both significantly higher than those in two cycles of soaking (2C-HA) and three cycles of soaking (3C-HA) groups. The transcription levels of specific genes (runx2 and osteonectin) in osteoblasts of 1C-HA group were significantly higher than those of N-HA group. Moreover, the levels showed no significant difference among 1C-HA, 2C-HA, and 3C-HA groups. In conclusion, microporous silk scaffold with 1 cycle of HA-coating can combine the biocompatibility of silk and osteogenicity of HA.
authors.affiliation: Institute of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China ; Department of Orthopaedics, 513 Hospital of PLA, Lanzhou 732750, China.
authors.forename: Hongguo
authors.identifier: 0000-0003-3047-1922
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Science, Air Force Engineering University, Xi'an 710032, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Institute of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Liguo
authors.identifier: 0000-0001-5848-4181
authors.initials: L
authors.lastname: Sun
authors.affiliation: Institute of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yingsen
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Military Medical Training, Comprehensive Training Base of Lanzhou, Hutubi 831200, China.
authors.forename: Zhangying
authors.initials: Z
authors.lastname: Hao
authors.affiliation: Institute of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Institute of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xiangli
authors.initials: X
authors.lastname: Fan
authors.affiliation: Institute of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Fan
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects*; D016062:Porosity; D011817:Rabbits; D047011:Silk / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D047011:Silk; D017886:Durapatite
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