title: First haploid genetic map based on microsatellite markers in Senegalese sole (Solea senegalensis, Kaup 1858).
abstract: The Senegalese sole (Solea senegalensis, Kaup 1858) is a flatfish species of great value for aquaculture. In this study, we develop the first linkage map in this species based on microsatellite markers characterized from genomic DNA libraries and EST databases of Senegalese sole and from other flatfish species. Three reference gynogenetic families were obtained by chromosome-manipulation techniques: two haploid gynogenetics, used to assign and order microsatellites to linkage groups and another diploid gynogenetic family, used for estimating marker-centromere distances. The consensus map consists of 129 microsatellites distributed in 27 linkage groups (LG), with an average density of 4.7 markers per LG and comprising 1,004 centimorgans (cM). Additionally, 15 markers remained unlinked. Through half-tetrad analysis, we were able to estimate the centromere distance for 81 markers belonging to 24 LG, representing an average of 3 markers per LG. Comparative mapping was performed between flatfish species LG and model fish species chromosomes (stickleback, Tetraodon, medaka, fugu and zebrafish). The usefulness of microsatellite markers and the genetic map as tools for comparative mapping and evolution studies is discussed.
authors.affiliation: Facultad de Ciencias, Departamento de Genética, Universidad de Granada, 18071, Granada, Spain.
authors.forename: Ma Jesús
authors.initials: MJ
authors.lastname: Molina-Luzón
authors.forename: Miguel
authors.initials: M
authors.lastname: Hermida
authors.forename: Rafael
authors.initials: R
authors.lastname: Navajas-Pérez
authors.forename: Francisca
authors.initials: F
authors.lastname: Robles
authors.forename: José Ignacio
authors.initials: JI
authors.lastname: Navas
authors.forename: Carmelo
authors.initials: C
authors.lastname: Ruiz-Rejón
authors.forename: Carmen
authors.initials: C
authors.lastname: Bouza
authors.forename: Paulino
authors.initials: P
authors.lastname: Martínez
authors.forename: Roberto
authors.initials: R
authors.lastname: de la Herrán
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods / Q000662:veterinary*; D005412:Flatfishes / Q000235:genetics*; D006238:Haploidy; D018895:Microsatellite Repeats / Q000235:genetics*; D013045:Species Specificity
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