title: [Effect of supplementing Qi and activating blood circulation method on protein uptake in NRK-52E cells injured by high glucose].
abstract: OBJECTIVE
  To study the effect of supplementing Qi and activating blood circulation method with danshen and huangqi on protein uptake in NRK-52E cells injured by high glucose.
  
  
  METHODS
  The influence of combined use of huangqi and danshen on NRK-52E cells proliferation were determined by MTT method, FITC-BSA uptake were observed by fluorescence microscope and analyzed by fluorescence spectrophotometer, and megalin expression were detected by the method of immunohistochemistry.
  
  
  RESULTS
  Combined use of huangqi and danshen could strengthen its protection effect. The FITC-BSA uptake and expression of megalin in NRK-52E cells were decreased in high glucose environment. Combined use of huangqi and danshen could increase FITC-BSA uptake and megalin expression in high glucose-injured NRK-52E cells.
  
  
  CONCLUSIONS
  The method of supplementing Qi and activating blood circulation can protect high glucose injured NRK-52E cells and improve the function of protein uptake by increasing expression of megalin.
authors.forename: Deng-ke
authors.initials: DK
authors.lastname: Yin
authors.forename: Ye
authors.initials: Y
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D049109:Cell Proliferation; D002478:Cells, Cultured; D003928:Diabetic Nephropathies / Q000378:metabolism / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005947:Glucose / Q000009:adverse effects*; D007150:Immunohistochemistry; D007684:Kidney Tubules / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D027561:Salvia miltiorrhiza / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014227:Triazines / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D014227:Triazines; C028972:dan-shen root extract; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate; D005947:Glucose; C011907:melamine