title: Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells.
abstract: Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is a causative agent of porcine enteric disease characterized by acute watery diarrhea and dehydration in sucking piglet. Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular receptors and inducing membrane fusion between viral envelopes and cellular membranes. However, the entry mechanism of PEDV is not studied. Here, we determined the entry mechanism of PEDV into Vero cells. Our data confirmed that PEDV entry followed clathrin-mediated endocytosis independence of caveolae-coated pit assembly. The internalized PEDV was co-localized with the clathrin-mediated endocytic marker, but not with the caveolae-mediated endocytic marker. In addition, cells treated with lysosomotropic agents and serine protease inhibitors were resistant to PEDV. Our data revealed that PEDV entry followed clathrin-mediated endocytosis and was dependent on a low pH and serine proteolysis for successful entry into cells.
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, South Korea.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Park
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, South Korea.
authors.forename: Deu John M
authors.initials: DJ
authors.lastname: Cruz
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, South Korea; Research Institute of Veterinary Medicine, Chungnam National University, South Korea. Electronic address: shin0089@cnu.ac.kr.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Shin
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D002966:Clathrin / Q000378:metabolism*; D018352:Coronavirus Infections / Q000201:enzymology / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D004705:Endocytosis; D053485:Porcine epidemic diarrhea virus / Q000502:physiology*; D012697:Serine Endopeptidases / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000201:enzymology* / Q000378:metabolism / Q000821:virology; D014709:Vero Cells; D053586:Virus Internalization
chemical_list: D002966:Clathrin; D012697:Serine Endopeptidases
keywords: Clathrin; Coronavirus; Entry; Low pH; Porcine epidemic diarrhea; Serine protease
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