title: In-silico identification and characterization of organic and inorganic chemical stress responding genes in yeast (Saccharomyces cerevisiae).
abstract: To study the life processes of all eukaryotes, yeast (Saccharomyces cerevisiae) is a significant model organism. It is also one of the best models to study the responses of genes at transcriptional level. In a living organism, gene expression is changed by chemical stresses. The genes that give response to chemical stresses will provide good source for the strategies in engineering and formulating mechanisms which are chemical stress resistant in the eukaryotic organisms. The data available through microarray under the chemical stresses like lithium chloride, lactic acid, weak organic acids and tomatidine were studied by using computational tools. Out of 9335 yeast genes, 388 chemical stress responding genes were identified and characterized under different chemical stresses. Some of these are: Enolases 1 and 2, heat shock protein-82, Yeast Elongation Factor 3, Beta Glucanase Protein, Histone H2A1 and Histone H2A2 Proteins, Benign Prostatic Hyperplasia, ras GTPase activating protein, Establishes Silent Chromatin protein, Mei5 Protein, Nondisjunction Protein and Specific Mitogen Activated Protein Kinase. Characterization of these genes was also made on the basis of their molecular functions, biological processes and cellular components.
authors.affiliation: Department of Botany, University of Balochistan, Quetta, Pakistan. Electronic address: barozaikhan@gmail.com.
authors.forename: Muhammad Younas Khan
authors.initials: MY
authors.lastname: Barozai
authors.affiliation: Department of Chemistry, Sardar Bahadur Khan Women's University, Quetta, Pakistan.
authors.forename: Farrukh
authors.initials: F
authors.lastname: Bashir
authors.affiliation: Department of Chemistry, Sardar Bahadur Khan Women's University, Quetta, Pakistan.
authors.forename: Shafia
authors.initials: S
authors.lastname: Muzaffar
authors.affiliation: Department of Chemistry, Sardar Bahadur Khan Women's University, Quetta, Pakistan.
authors.forename: Saba
authors.initials: S
authors.lastname: Afzal
authors.affiliation: Department of Chemistry, Sardar Bahadur Khan Women's University, Quetta, Pakistan.
authors.forename: Farida
authors.initials: F
authors.lastname: Behlil
authors.affiliation: Department of Chemistry, Sardar Bahadur Khan Women's University, Quetta, Pakistan.
authors.forename: Muzaffar
authors.initials: M
authors.lastname: Khan
mesh_terms: D003198:Computer Simulation; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D063990:Gene Ontology; D005800:Genes, Fungal / Q000235:genetics; D007287:Inorganic Chemicals / Q000494:pharmacology*; D019344:Lactic Acid / Q000494:pharmacology; D018021:Lithium Chloride / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D009930:Organic Chemicals / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D014053:Tomatine / Q000031:analogs & derivatives / Q000494:pharmacology; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics
chemical_list: D007287:Inorganic Chemicals; D009930:Organic Chemicals; D029701:Saccharomyces cerevisiae Proteins; C003677:tomatidine; D014053:Tomatine; D019344:Lactic Acid; D018021:Lithium Chloride
keywords: Chemical stresses; Differentially expressed genes; Saccharomyces cerevisiae