title: Basic fibroblastic growth factor affects the osteogenic differentiation of dental pulp stem cells in a treatment-dependent manner.
abstract: OBJECTIVE
  To determine how basic fibroblastic growth factor (bFGF) affected the osteogenic differentiation of human dental pulp stem cells (DPSCs) in vitro and in vivo.
  
  
  METHODS
  Basic fibroblastic growth factor stimulation of DPSCs was divided into a pre-treatment period and an osteogenic differentiation period. Alizarin red quantification experiments and alkaline phosphatase activity quantification assay were performed to examine the osteogenic differentiation of DPSCs after different bFGF stimulation. Quantification reverse transcription polymerase chain reaction was used to analyze the osteogenic gene expression of DPSCs after different bFGF stimulation. In addition, DPSCs that received the 1 and 2 weeks bFGF pre-treatments as in the in vitro experiments were mineralized for 1 week and seeded into hydroxyapatite/tricalcium phosphate (HA/TCP) pills and subcutaneously transplanted into naked mice for 2 or 3 months. The transplants were removed, sliced and stained using Modified Ponceau Trichrome Stain to observe the formation of mineralized tissue.
  
  
  RESULTS
  Basic fibroblastic growth factor stimulation in the osteogenic differentiation period decreased the in vitro osteogenic differentiation ability of DPSCs. One week pre-treatment with bFGF increased the in vitro osteogenic differentiation ability of DPSCs, whereas 2 weeks pre-treatment with bFGF decreased the in vitro osteogenic differentiation ability of DPSCs. The pre-treatment period was vital for the osteogenic differentiation of DPSCs in vitro. The in vivo results were similar to the in vitro results.
  
  
  CONCLUSIONS
  Basic fibroblastic growth factor affected the osteogenic differentiation of DPSCs in a treatment-dependent manner both in vitro and in vivo.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: J
authors.initials: J
authors.lastname: Qian
authors.affiliation: Hospital of Stomatology, The Zunyi Medical University, Zunyi, Guizhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Jiayuan
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: J
authors.initials: J
authors.lastname: Wenkai
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: W
authors.initials: W
authors.lastname: Peina
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Ansheng
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: S
authors.initials: S
authors.lastname: Shukai
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Shafei
authors.affiliation: Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, Ann Arbor, MI, USA.
authors.forename: L
authors.initials: L
authors.lastname: Jun
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: N
authors.initials: N
authors.lastname: Longxing
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008964:Molar, Third; D010012:Osteogenesis / Q000187:drug effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D016222:Fibroblast Growth Factor 2; D000469:Alkaline Phosphatase
keywords: basic fibroblast growth factor; dental pulp stem cells; osteogenic differentiation