title: Culturable bacterial microbiota of the stomach of Helicobacter pylori positive and negative gastric disease patients.
abstract: Human stomach is the only known natural habitat of Helicobacter pylori (Hp), a major bacterial pathogen that causes different gastroduodenal diseases. Despite this, the impact of Hp on the diversity and the composition of the gastric microbiota has been poorly studied. In this study, we have analyzed the culturable gastric microbiota of 215 Malaysian patients, including 131 Hp positive and 84 Hp negative individuals that were affected by different gastric diseases. Non-Hp bacteria isolated from biopsy samples were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry based biotyping and 16SrRNA sequencing. The presence of Hp did not significantly modify the diversity of the gastric microbiota. However, correlation was observed between the isolation of Streptococci and peptic ulcer disease. In addition, as a first report, Burkholderia pseudomallei was also isolated from the gastric samples of the local population. This study suggested that there may be geographical variations in the diversity of the human gastric microbiome. Geographically linked diversity in the gastric microbiome and possible interactions between Hp and other bacterial species from stomach microbiota in pathogenesis are proposed for further investigations.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yalda
authors.initials: Y
authors.lastname: Khosravi
authors.affiliation: Vice-chancellor's Office, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yakhya
authors.initials: Y
authors.lastname: Dieye
authors.affiliation: BP Diagnostics Centre Sdn Bhd, 30250 Ipoh, Perak, Malaysia.
authors.forename: Bee Hoon
authors.initials: BH
authors.lastname: Poh
authors.affiliation: Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545.
authors.forename: Chow Goon
authors.initials: CG
authors.lastname: Ng
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mun Fai
authors.initials: MF
authors.lastname: Loke
authors.affiliation: Department of Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Khean Lee
authors.initials: KL
authors.lastname: Goh
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Jamuna
authors.initials: J
authors.lastname: Vadivelu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D064307:Microbiota / Q000235:genetics; D008875:Middle Aged; D012336:RNA, Ribosomal, 16S; D013270:Stomach / Q000382:microbiology* / Q000473:pathology; D013272:Stomach Diseases / Q000382:microbiology; D055815:Young Adult
chemical_list: D012336:RNA, Ribosomal, 16S
references.citation: Helicobacter. 1996 Dec;1(4):207-18
references.pmid: 9398871
references.citation: Clin Microbiol Rev. 2010 Apr;23(2):382-98
references.pmid: 20375358
references.citation: Sci Rep. 2011;1:170
references.pmid: 22355685
references.citation: Gastrointest Endosc Clin N Am. 2011 Oct;21(4):613-35
references.pmid: 21944414
references.citation: Proc Natl Acad Sci U S A. 2006 Jan 17;103(3):732-7
references.pmid: 16407106
references.citation: Nat Rev Microbiol. 2013 Jun;11(6):385-99
references.pmid: 23652324
references.citation: Nature. 2012 Sep 13;489(7415):220-30
references.pmid: 22972295
references.citation: Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):5024-9
references.pmid: 15044704
references.citation: J Clin Microbiol. 2013 May;51(5):1639-40
references.pmid: 23447635
references.citation: Best Pract Res Clin Gastroenterol. 2013 Feb;27(1):39-45
references.pmid: 23768551
references.citation: Gut Pathog. 2010 Mar 31;2(1):2
references.pmid: 20356368
references.citation: Cell. 2010 May 28;141(5):822-33
references.pmid: 20510929
references.citation: Infect Immun. 2002 Apr;70(4):1984-90
references.pmid: 11895962
references.citation: Sci Rep. 2014 Feb 26;4:4202
references.pmid: 24569566
references.citation: J Clin Microbiol. 1989 Sep;27(9):2080-2
references.pmid: 2778071
references.citation: ISME J. 2011 Apr;5(4):574-9
references.pmid: 20927139
references.citation: Nat Rev Neurosci. 2012 Oct;13(10):701-12
references.pmid: 22968153
references.citation: Nature. 2010 Mar 4;464(7285):59-65
references.pmid: 20203603
references.citation: J Med Microbiol. 2009 Apr;58(Pt 4):509-516
references.pmid: 19273648
references.citation: Annu Rev Med. 2011;62:361-80
references.pmid: 21226616
references.citation: PLoS One. 2009 Nov 24;4(11):e7985
references.pmid: 19956741
references.citation: PLoS One. 2008 Jul 30;3(7):e2836
references.pmid: 18665274
references.citation: Front Cell Infect Microbiol. 2012 Feb 16;2:10
references.pmid: 22919602
references.citation: J Microbiol Methods. 2007 May;69(2):330-9
references.pmid: 17391789
references.citation: Cell. 2012 Jun 22;149(7):1578-93
references.pmid: 22726443
references.citation: Gastroenterology. 2007 Aug;133(2):659-72
references.pmid: 17681184
references.citation: Infect Immun. 1996 Jul;64(7):2445-8
references.pmid: 8698465
references.citation: PLoS One. 2010 Jan 20;5(1):e8804
references.pmid: 20098711
references.citation: Nature. 2012 Aug 9;488(7410):178-84
references.pmid: 22797518
references.citation: PLoS Pathog. 2012;8(5):e1002693
references.pmid: 22589724
references.citation: World J Gastroenterol. 2012 Mar 21;18(11):1257-61
references.pmid: 22468090
references.citation: Cell. 2012 Aug 3;150(3):470-80
references.pmid: 22863002
references.citation: Mol Microbiol. 2010 Nov;78(4):809-19
references.pmid: 20815828
references.citation: Physiol Rev. 2010 Jul;90(3):859-904
references.pmid: 20664075
references.citation: J Clin Invest. 2004 Feb;113(3):321-33
references.pmid: 14755326
references.citation: PLoS One. 2012;7(5):e37324
references.pmid: 22624016