title: Identification of a group of cryptic marine limpet species, Cellana karachiensis (Mollusca: Patellogastropoda) off Veraval coast, India, using mtDNA COI sequencing.
abstract: Present communication reports the phylogenetic relationship between three groups of a marine limpet having different color banding patterns using COI sequencing. Samples were sequenced for mtDNA COI gene using universal primer. Comparative BLAST revealed that all three types were around 99.59% identical with Cellana karachiensis, first record of this species from Indian coasts. Apart from the morphological variations, the mtDNA COI gene analysis revealed around 1% nucleotide variations between these three types. The observed dissimilarity in COI sequences was possibly too little to consider these types as three different species. The derivation of amino acid positions indicated that these types could possibly be a complex of three cryptic species of C. karachiensis. The study proposes that the Oman and Indian populations of C. karachiensis might have derived by allopatric speciation due to geographical isolation. The group of these three cryptic species, sharing same habitat between themselves, possibly showed sympatric speciation.
authors.affiliation: a Department of Biosciences , Saurashtra University , Rajkot , Gujarat , India.
authors.forename: Sneha
authors.initials: S
authors.lastname: Joseph
authors.affiliation: a Department of Biosciences , Saurashtra University , Rajkot , Gujarat , India.
authors.forename: Paresh
authors.initials: P
authors.lastname: Poriya
authors.affiliation: a Department of Biosciences , Saurashtra University , Rajkot , Gujarat , India.
authors.forename: Bhavik
authors.initials: B
authors.lastname: Vakani
authors.affiliation: a Department of Biosciences , Saurashtra University , Rajkot , Gujarat , India.
authors.forename: S P
authors.initials: SP
authors.lastname: Singh
authors.affiliation: a Department of Biosciences , Saurashtra University , Rajkot , Gujarat , India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Kundu
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D049851:Gastropoda / Q000145:classification / Q000235:genetics*; D049810:Genetic Speciation; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV
keywords: Color banding pattern; cryptic species; limpet; mtDNA COI; taxonomy