title: Clinicopathological and prognostic significance of p53, Ki-67, and Bcl-2 expression in Tunisian gastric adenocarcinomas.
abstract: Gastric carcinoma (GC) is a highly aggressive malignancy with poor prognosis. It is widely accepted that malignancy results from abnormal cell growth due to dysregulation of the balance between cell proliferation and apoptosis. Our study aimed to investigate the clinicopathological and prognostic significance of p53, Ki-67, and Bcl-2 in Tunisian GC patients by immunohistochemistry. It was observed that the older patients showed p53 overexpression compared with the younger patients (p<0.05). There was higher p53 expression in the intestinal-type compared with the diffuse-type (p<0.05), and in well/moderate differentiated than in poor differentiated tumors. The expression of Ki-67 was positively associated with tumor size and venous invasion (p<0.05). Bcl2 expression occurred in male patients and correlated with depth of invasion (p=0.02). A Kaplan-Meier analysis indicated an inverse correlation between p53 and Ki-67 expression and the overall survival. Multivariate analysis revealed that the tumor site, Ki-67 and p53 expression were independent prognostic factors for gastric carcinomas (p<0.05). Finally, combined expression of p53, Ki-67 and Bcl-2 showed that the group of patients with tumors p53+/Ki-67+/Bcl2- had aggressive behavior and poor prognosis (p log rank=0.000). In summary, our data indicated that the expression of p53, Ki-67, and Bcl-2 may provide useful information for identifying patients with aggressive behavior and poor prognosis of GC.
authors.affiliation: Center of Biotechnology of Sfax, University of Sfax, BP K 3018, Sfax, Tunisia; Department of Anatomy-Pathology, Habib Bourguiba Hospital, Sfax, Tunisia. Electronic address: dorra.benayed@gmail.com.
authors.forename: Dorra Ben
authors.initials: DB
authors.lastname: Ayed
authors.affiliation: Department of Anatomy-Pathology, Habib Bourguiba Hospital, Sfax, Tunisia.
authors.forename: Abdelmajid
authors.initials: A
authors.lastname: Khabir
authors.affiliation: Department of Surgery, Habib Bourguiba Hospital, Sfax, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abid
authors.affiliation: Department of Surgery, Habib Bourguiba Hospital, Sfax, Tunisia.
authors.forename: Mohamed Issam
authors.initials: MI
authors.lastname: Bayrouti
authors.affiliation: Center of Biotechnology of Sfax, University of Sfax, BP K 3018, Sfax, Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Gargouri
authors.affiliation: Department of Anatomy-Pathology, Habib Bourguiba Hospital, Sfax, Tunisia.
authors.forename: Tahia
authors.initials: T
authors.lastname: Sellami-Boudawara
authors.affiliation: Center of Biotechnology of Sfax, University of Sfax, BP K 3018, Sfax, Tunisia. Electronic address: raja.gargouri@cbs.rnrt.tn.
authors.forename: Raja
authors.initials: R
authors.lastname: Mokdad-Gargouri
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014416:Tunisia; D055815:Young Adult
chemical_list: D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53
keywords: Bcl-2; Gastric carcinoma; Prognosis; ki-67; p53