title: Detection of unamplified genomic DNA by a PNA-based microstructured optical fiber (MOF) Bragg-grating optofluidic system.
abstract: Microstructured optical fibers containing microchannels and Bragg grating inscribed were internally functionalized with a peptide nucleic acid (PNA) probe specific for a gene tract of the genetically modified Roundup Ready soy. These fibers were used as an optofluidic device for the detection of DNA by measuring the shift in the wavelength of the reflected IR light. Enhancement of optical read-out was obtained using streptavidin coated gold-nanoparticles interacting with the genomic DNA captured in the fiber channels (0%, 0.1%, 1% and 10% RR-Soy), enabling to achieve statistically significant, label-free, and amplification-free detection of target DNA in low concentrations, low percentages, and very low sample volumes. Computer simulations of the fiber optics based on the finite element method (FEM) were consistent with the formation of a layer of organic material with an average thickness of 39 nm for the highest percentage (10% RR soy) analysed.
authors.affiliation: Dipartimento di Chimica, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bertucci
authors.affiliation: Dipartimento di Chimica, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy.
authors.forename: Alex
authors.initials: A
authors.lastname: Manicardi
authors.affiliation: Dipartimento di Ingegneria dell'Informazione, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Candiani
authors.affiliation: Dipartimento di Ingegneria dell'Informazione, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Giannetti
authors.affiliation: Dipartimento di Ingegneria dell'Informazione, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Cucinotta
authors.affiliation: Dipartimento di Chimica, Università di Catania, Via Andrea Doria 6, 95125 Catania, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spoto
authors.affiliation: Foundation for Research and Technology Hellas (FORTH), Institute of Electronic Structure and Laser (IESL), P.O. Box 1385, Heraklion 71 110, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Konstantaki
authors.affiliation: Foundation for Research and Technology Hellas (FORTH), Institute of Electronic Structure and Laser (IESL), P.O. Box 1385, Heraklion 71 110, Greece.
authors.forename: Stavros
authors.initials: S
authors.lastname: Pissadakis
authors.affiliation: Dipartimento di Ingegneria dell'Informazione, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy. Electronic address: stefano.selleri@unipr.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Selleri
authors.affiliation: Dipartimento di Chimica, Università di Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy. Electronic address: roberto.corradini@unipr.it.
authors.forename: Roberto
authors.initials: R
authors.lastname: Corradini
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D002874:Chromosome Mapping / Q000295:instrumentation; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008904:Miniaturization; D021141:Nucleic Acid Amplification Techniques; D055096:Optical Devices; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000235:genetics*; D012031:Refractometry / Q000295:instrumentation*
chemical_list: D020135:Peptide Nucleic Acids; D004247:DNA
keywords: Biophotonics; DNA detection; Optofluidics; Peptide nucleic acids; Photonic crystal fibers