title: Identification of immuno-reactive capsid proteins of malignant catarrhal fever viruses.
abstract: Malignant catarrhal fever (MCF) is a fatal disease of cattle and other ungulates caused by certain gamma-herpesviruses including alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). An attenuated virus vaccine based on AlHV-1 has been shown to induce virus-neutralising antibodies in plasma and nasal secretions of protected cattle but the targets of virus-specific antibodies are unknown. Proteomic analysis and western blotting of virus extracts allowed the identification of eight candidate AlHV-1 virion antigens. Recombinant expression of selected candidates and their OvHV-2 orthologues confirmed that two polypeptides, the products of the ORF17.5 and ORF65 genes, were antigens recognised by antibodies from natural MCF cases or from AlHV-1 vaccinated cattle. These proteins have potential as diagnostic and/or vaccine antigens.
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Bartley
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.
authors.forename: David
authors.initials: D
authors.lastname: Deane
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.
authors.forename: Ann
authors.initials: A
authors.lastname: Percival
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.
authors.forename: Inga R
authors.initials: IR
authors.lastname: Dry
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.
authors.forename: Dawn M
authors.initials: DM
authors.lastname: Grant
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.
authors.forename: Neil F
authors.initials: NF
authors.lastname: Inglis
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.
authors.forename: Kevin
authors.initials: K
authors.lastname: Mclean
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.
authors.forename: Erin D T
authors.initials: ED
authors.lastname: Manson
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.
authors.forename: Lisa H J
authors.initials: LH
authors.lastname: Imrie
authors.affiliation: School of Veterinary Medicine and Science, Nottingham University, Sutton Bonington, Leicestershire LE12 5RD, UK.
authors.forename: David M
authors.initials: DM
authors.lastname: Haig
authors.affiliation: Institute for Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, UK; Paul G. Allen School of Global Animal Health, Washington State University, USA.
authors.forename: Felix
authors.initials: F
authors.lastname: Lankester
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK. Electronic address: george.russell@moredun.ac.uk.
authors.forename: George C
authors.initials: GC
authors.lastname: Russell
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000097:blood*; D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D006564:Herpesviridae / Q000235:genetics / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D008304:Malignant Catarrh / Q000276:immunology* / Q000517:prevention & control; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014771:Virion / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
keywords: Antibodies; Antigen identification; Inflammatory disease; Malignant catarrhal fever; Proteomics; Recombinant expression