title: Impact of rli87 gene deletion on response of Listeria monocytogenes to environmental stress.
abstract: Listeria monocytogenes (LM) is a zoonotic pathogen that widely adapts to various environments. Recent studies have found that noncoding RNAs (ncRNAs) play regulatory roles in LM responses to environmental stress. To understand the role of ncRNA rli87 in the response regulation, a rli87 deletion strain LM-Δrli87 was constructed by homologous recombination and tested for stress responses to high temperature, low temperature, high osmotic pressure, alcohol, acidity, alkaline and oxidative environments, along with LM EGD-e strain (control). The results showed that compared with LM EGD-e, LM-Δrli87 grew faster (P < 0.05) at low temperature (30 °C), high temperature (42 °C), and in alkaline condition (pH = 9), similarly (P > 0.05) in acidic and high osmatic pressure (10% NaCl) conditions. When cultured in medium containing 3.8% ethanol, the growth was not significantly different between the two strains (P > 0.05). When cultured at pH 9, they had similar growth rates in the first 5 h (P > 0.05), but the rates were significantly different after 6 h (P < 0.05). The expression of rsbV, rsbW, hpt, clpP, and ctsR was upregulated in LM-∆rli87 compared with LM EGD-e at pH 9, indicating that the rli87 gene regulated the expression of the five genes in alkaline environment. Our results suggest that the rli87 gene has an important regulatory role in LM's response to temperature (30 and 42 °C), alkaline stresses.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Kun
authors.forename: Meng
authors.initials: M
authors.lastname: Qingling
authors.forename: Jun
authors.initials: J
authors.lastname: Qiao
authors.forename: Peng
authors.initials: P
authors.lastname: Yelong
authors.forename: Liu
authors.initials: L
authors.lastname: Tianli
authors.forename: Chen
authors.initials: C
authors.lastname: Cheng
authors.forename: Ma
authors.initials: M
authors.lastname: Yu
authors.forename: Hu
authors.initials: H
authors.lastname: Zhengxiang
authors.forename: Cai
authors.initials: C
authors.lastname: Xuepeng
authors.forename: Chen
authors.initials: C
authors.lastname: Chuangfu
mesh_terms: D000143:Acids / Q000633:toxicity; D000438:Alcohols / Q000633:toxicity; D000468:Alkalies / Q000633:toxicity; D003470:Culture Media / Q000737:chemistry; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D009997:Osmotic Pressure; D018384:Oxidative Stress; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological; D013696:Temperature
chemical_list: D000143:Acids; D000438:Alcohols; D000468:Alkalies; D003470:Culture Media; D012329:RNA, Bacterial; D022661:RNA, Untranslated; D012965:Sodium Chloride
keywords: Listeria monocytogenes; environmental stress; homologous recombination; ncRNA rli87 gene