title: Bdellovibrio and like organisms enhanced growth and survival of Penaeus monodon and altered bacterial community structures in its rearing water.
abstract: In this study, a 96-h laboratory reduction test was conducted with strain BDHSH06 (GenBank accession no. EF011103) as the test strain for Bdellovibrio and like organisms (BALOs) and 20 susceptible marine bacterial strains forming microcosms as the targets. The results showed that BDHSH06 reduced the levels of approximately 50% of prey bacterial strains within 96 h in the seawater microcosms. An 85-day black tiger shrimp (Penaeus monodon) rearing experiment was performed. The shrimp survival rate, body length, and weight in the test tanks were 48.1% ± 1.2%, 99.8 ± 10.0 mm, and 6.36 ± 1.50 g, respectively, which were values significantly (P < 0.05) higher than those for the control, viz., 31.0% ± 2.1%, 86.0 ± 11.1 mm, and 4.21 ± 1.56 g, respectively. With the addition of BDHSH06, total bacterial and Vibrio numbers were significantly reduced (P < 0.05) by 1.3 to 4.5 log CFU · ml(-1) and CFU · g(-1) in both water and shrimp intestines, respectively, compared to those in the control. The effect of BDHSH06 on bacterial community structures in the rearing water was also examined using PCR amplification of the 16S rRNA gene and denaturing gradient gel electrophoresis (DGGE). The DGGE profiles of rearing water samples from the control and test tanks revealed that the amounts of 44% of the bacterial species were reduced when BDHSH06 was added to the rearing water over the 85-day rearing period, and among these, approximately 57.1% were nonculturable. The results of this study demonstrated that BDHSH06 can be used as a biocontrol/probiotic agent in P. monodon culture.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Biosciences and Bioengineering, South China University of Technology, Guangzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Sun
authors.affiliation: College of Biosciences and Bioengineering, South China University of Technology, Guangzhou, China.
authors.forename: Renliang
authors.initials: R
authors.lastname: Liu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China College of Biosciences and Bioengineering, South China University of Technology, Guangzhou, China febjpcai@scut.edu.cn.
authors.forename: Junpeng
authors.initials: J
authors.lastname: Cai
mesh_terms: D000818:Animals; D017756:Aquaculture; D001501:Bdellovibrio / Q000502:physiology*; D061046:Biological Control Agents; D001835:Body Weight; D058645:Denaturing Gradient Gel Electrophoresis; D033561:Penaeidae / Q000254:growth & development* / Q000382:microbiology* / Q000502:physiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D015996:Survival Rate; D014871:Water Microbiology
chemical_list: D061046:Biological Control Agents; D012336:RNA, Ribosomal, 16S
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