title: Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.
abstract: We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.
authors.affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Haramizu
authors.affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Mori
authors.affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Yano
authors.affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.
authors.forename: Noriyasu
authors.initials: N
authors.lastname: Ota
authors.affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.
authors.forename: Kohjiro
authors.initials: K
authors.lastname: Hashizume
authors.affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Otsuka
authors.affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hase
authors.affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Shimotoyodome
keywords: Aging; Milk fat globule membrane; Muscle function; Neuromuscular junction
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