title: [Effect of total saponins from Sanguisorba officinalis on megakaryocyte progenitor cells proliferation, differentiation and relative receptor expression].
abstract: OBJECTIVE
  To investigate the effects of total saponins from Sanguisorba officinalis (DYS) on hematopoietic cell proliferation, differentiation and the expression level of IL-3R and c-kit.
  
  
  METHODS
  Baf3 and 32D cells were cultured with or without IL-3, then the cells were exposed to DYS in different concentrations of 5, 10, 20, 30 and 40 mg x L(-1) for 24, 48, 72 and 96 hours separately. After that, the cell proliferation and differentiation capacity were determinated by the methods of CCK8 and Giemsa staining separately. The effects of DYS on the expression level of IL-3 receptor in Baf3 cells and the expression level of c-kit in 32D cells were determinated using RT-PCR.
  
  
  RESULTS
  DYS promotes alone proliferation of Baf3 cells and 32D cells after 48 h. In contrast to control cells, 32D cells containing DYS without IL-3 form many large clusters. DYS also increases the proliferation when cultured with IL-3. High concentration of DYS induce alone the differentiation of 32D cells and increase alone the number of the polyploidy megakaryocyte. Moreover, DYS increases alone the expression level of IL-3R in Baf3 cells and the expression level of c-kit in 32D cells separately.
  
  
  CONCLUSIONS
  Our data shows DYS can promote alone proliferation and differentiation of megakaryocyte progenitor cells. The proliferative and differentiative effect of DYS on megakaryocyte progenitor cells is correlated to the up-regulation of IL-3 receptor and c-kit expression.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Dai
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Gao
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Wu
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Fei-Hong
authors.initials: FH
authors.lastname: Huang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Zou
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D007377:Interleukin-3 / Q000494:pharmacology; D055016:Megakaryocyte Progenitor Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D016185:Receptors, Interleukin-3 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D031993:Sanguisorba / Q000737:chemistry*; D012503:Saponins / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D007377:Interleukin-3; D016185:Receptors, Interleukin-3; D012503:Saponins; D019009:Proto-Oncogene Proteins c-kit