title: The upregulated expression of OX40/OX40L and their promotion of T cells proliferation in the murine model of asthma.
abstract: OBJECTIVE
  To investigate whether the expression of OX40/OX40 ligand (OX40L) was upregulated in a murine model of asthma and their significance in the pathogenesis of asthma.
  
  
  METHODS
  After an ovalbumin-sensitized/challenged murine model of asthma was established, the expressions of OX40, OX40L in peripheral blood mononuclear cells (PBMCs) and bronchoalveolar lavage fluid (BALF) cell pellets were measured. Then T cell proliferation was analyzed by cell counting kit-8 (CCK8), and the protein levels of OX40 and OX40L in the lungs were determined by immunohistochemistry. The concentrations of IL-4 and IFN-γ in BALF and T cell culture supernatant were evaluated by ELISA.
  
  
  RESULTS
  The percentages of CD4(+)OX40(+), CD19(+)OX40L(+), F4/80(+)OX40L(+) in PBMCs and BALF cell pellets were higher in asthma group than in control group (all P<0.01). The proliferation capacity of T cells in asthma group was higher than that in control group (P<0.05). In asthma group, stimulation of OX40 by anti-OX40 mAb obviously promoted T cell proliferation and secretion of IL-4 and IFN-γ. Immunohistochemistry assay showed that OX40 and OX40L protein levels were higher in asthma group than those in control group (all P<0.05).
  
  
  CONCLUSIONS
  The expressions of OX40 and OX40L were upregulated in the murine asthmatic model. The upregulation of OX40/OX40L signals could induce the proliferation and cytokines secretion of T cells in asthmatic mice, indicating that OX40/OX40L signal was involved in the pathogenesis of asthma.
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China ; 2 Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou 215003, China ; 3 Institute of Medical Biotechnology of Soochow University, Suzhou 215007, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lei
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China ; 2 Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou 215003, China ; 3 Institute of Medical Biotechnology of Soochow University, Suzhou 215007, China.
authors.forename: Da-Xiong
authors.initials: DX
authors.lastname: Zeng
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China ; 2 Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou 215003, China ; 3 Institute of Medical Biotechnology of Soochow University, Suzhou 215007, China.
authors.forename: Can-Hong
authors.initials: CH
authors.lastname: Zhu
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China ; 2 Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou 215003, China ; 3 Institute of Medical Biotechnology of Soochow University, Suzhou 215007, China.
authors.forename: Gao-Qin
authors.initials: GQ
authors.lastname: Liu
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China ; 2 Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou 215003, China ; 3 Institute of Medical Biotechnology of Soochow University, Suzhou 215007, China.
authors.forename: Xiu-Qin
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China ; 2 Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou 215003, China ; 3 Institute of Medical Biotechnology of Soochow University, Suzhou 215007, China.
authors.forename: Chang-Guo
authors.initials: CG
authors.lastname: Wang
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China ; 2 Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou 215003, China ; 3 Institute of Medical Biotechnology of Soochow University, Suzhou 215007, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: 1 Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China ; 2 Department of Respiratory Medicine, Children's Hospital of Soochow University, Suzhou 215003, China ; 3 Institute of Medical Biotechnology of Soochow University, Suzhou 215007, China.
authors.forename: Jian-An
authors.initials: JA
authors.lastname: Huang
keywords: Asthma; OX40; OX40 ligand (OX40L); T cell proliferation; animal models
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