title: Enhanced cell adhesion and alignment on micro-wavy patterned surfaces.
abstract: Various micropatterns have been fabricated and used to regulate cell adhesion, morphology and function. Micropatterns created by standard photolithography process are usually rectangular channels with sharp corners (microgrooves) which provide limited control over cells and are not favorable for cell-cell interaction and communication. This paper proposes a new micropattern with smooth wavy surfaces (micro-waves) to control the position and orientation of cells. To characterize cell growth and responses on the micro-patterned substrates, bovine aortic endothelial cells were seeded onto surfaces with micro-grooves and micro-waves for 24 h. As a result, the cells on the micro-wavy pattern appeared to have a lower death rate and better alignment compared to those on the micro-grooved pattern. In addition, flow-induced shear stress was applied to examine the adhesion strength of cells on the micro-wavy pattern. Results showed that cells adhered to the wavy surface displayed both improved alignment and adhesion strength compared to those on the flat surface. The combination of increased alignment, lower death rate and enhanced adhesion strength of cells on the micro-wavy patterns will offer advantages in potential applications for cell phenotype, proliferation and tissue engineering.
authors.affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America.
authors.forename: Jia
authors.initials: J
authors.lastname: Hu
authors.affiliation: Bioengineering Program, Lehigh University, Bethlehem, Pennsylvania, United States of America.
authors.forename: Camille
authors.initials: C
authors.lastname: Hardy
authors.affiliation: Department of Material Science, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.
authors.forename: Chi-Mon
authors.initials: CM
authors.lastname: Chen
authors.affiliation: Department of Material Science, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.
authors.forename: Shu
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America; Bioengineering Program, Lehigh University, Bethlehem, Pennsylvania, United States of America.
authors.forename: Arkady S
authors.initials: AS
authors.lastname: Voloshin
authors.affiliation: Department of Mechanical Engineering and Mechanics, Lehigh University, Bethlehem, Pennsylvania, United States of America; Bioengineering Program, Lehigh University, Bethlehem, Pennsylvania, United States of America.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
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