title: Molecularly imprinted polymer nanocarriers for sustained release of erythromycin.
abstract: OBJECTIVE
  To develop and evaluate molecularly imprinted nanocarriers for sustained release of erythromycin in physiological buffer media.
  
  
  METHODS
  Erythromycin-imprinted poly(methacrylic acid-co-trimethylolpropane trimethacrylate) nanocarriers and corresponding control nanocarriers were prepared by free-radical precipitation polymerization. The nanocarriers were characterized by transmission electron microscopy, dynamic light scattering, and nitrogen sorption analysis. Binding studies were carried out with erythromycin and five structurally unrelated drugs. Molecular descriptors of the drugs were computed and correlated to measured binding data by multivariate data analysis. Loading with erythromycin and in vitro release studies were carried out in physiological buffer media. Kinetic models were fitted to drug release data.
  
  
  RESULTS
  The template affected the size and morphology of the nanocarriers. Binding isotherms showed that erythromycin-imprinted nanocarriers had a higher erythromycin binding capacity than corresponding control nanocarriers. Multivariate data analysis, correlating binding to molecular descriptors of the drugs, indicated a molecular imprinting effect. Erythromycin loading capacity was 76 mg/g with a loading efficiency of 87%. Release studies in physiological buffer showed an initial burst release of a quarter of loaded erythromycin during the first day and an 82% release after a week. The release was best described by the Korsmeyer-Peppas model.
  
  
  CONCLUSIONS
  Sustained release of erythromycin in physiological buffer was demonstrated.
authors.affiliation: Nanomedicine and Biomaterials, Department of Experimental Medical Science, Lund University, Biomedical Center, D11, 22184, Lund, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Kempe
authors.forename: Anna
authors.initials: A
authors.lastname: Parareda Pujolràs
authors.forename: Maria
authors.initials: M
authors.lastname: Kempe
mesh_terms: D003692:Delayed-Action Preparations / Q000737:chemistry / Q000378:metabolism; D004917:Erythromycin / Q000737:chemistry / Q000378:metabolism*; D054802:Molecular Imprinting / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry; D010316:Particle Size; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003692:Delayed-Action Preparations; D053769:Nanocapsules; D011108:Polymers; D004917:Erythromycin
references.citation: Anal Bioanal Chem. 2010 Feb;396(4):1599-606
references.pmid: 20012741
references.citation: J Comb Chem. 2003 Jan-Feb;5(1):67-72
references.pmid: 12523836
references.citation: Drug Chem Toxicol. 2001 May;24(2):191-200
references.pmid: 11360435
references.citation: Intern Med J. 2003 Mar;33(3):103-9
references.pmid: 12603583
references.citation: J Antibiot (Tokyo). 1997 Jan;50(1):89-91
references.pmid: 9066772
references.citation: Anal Chem. 2006 Mar 15;78(6):2019-27
references.pmid: 16536441
references.citation: AIChE J. 2009 Jun;55(6):1311-1324
references.pmid: 26500352
references.citation: Analyst. 2004 May;129(5):456-62
references.pmid: 15116240
references.citation: Pharmacol Ther. 2008 Mar;117(3):393-405
references.pmid: 18289694
references.citation: J Am Chem Soc. 2004 Jun 30;126(25):7827-33
references.pmid: 15212530
references.citation: J Mol Recognit. 2012 Jun;25(6):370-6
references.pmid: 22641535
references.citation: Anal Bioanal Chem. 2008 Apr;390(8):2141-50
references.pmid: 18368392
references.citation: Anal Chem. 1997 Jun 1;69(11):2017-21
references.pmid: 21639240
references.citation: J Biomater Sci Polym Ed. 2009;20(7-8):1065-79
references.pmid: 19454169
references.citation: Anal Bioanal Chem. 2010 Dec;398(7-8):3087-96
references.pmid: 20936264
references.citation: Biotechnol Annu Rev. 2006;12:225-68
references.pmid: 17045196
references.citation: J Rheumatol. 2004 Mar;31(3):436-41
references.pmid: 14994385
references.citation: Anal Chem. 1996 Jun 1;68(11):1948-53
references.pmid: 8686916
references.citation: Nanomedicine. 2011 Jun;7(3):284-94
references.pmid: 21059404
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Jun 1;879(19):1617-24
references.pmid: 21498135
references.citation: J Nanosci Nanotechnol. 2012 Sep;12(9):7388-94
references.pmid: 23035481
references.citation: J Mol Recognit. 2006 Mar-Apr;19(2):106-80
references.pmid: 16395662
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Apr 5;802(2):347-53
references.pmid: 15018797
references.citation: Clin Microbiol Rev. 2010 Jul;23(3):590-615
references.pmid: 20610825
references.citation: Cardiovasc Ther. 2012 Dec;30(6):301-7
references.pmid: 22136572
references.citation: Bone. 2009 Apr;44(4):671-7
references.pmid: 19154802
references.citation: Drugs. 2007;67(3):369-91
references.pmid: 17335296