title: Study of rust resistance genes in wheat germplasm with DNA markers.
abstract: Wheat is a vital dietary component for human health and widely consumed in the world. Wheat rusts are dangerous pathogens and contribute serious threat to its production. In present study, PCR-Based DNA Markers were employed to check the rust resistance genes among 20 wheat genotypes and 22 markers were amplified. NTSYS-pc 2.2 was used to calculate genetic diversity and Nei and Li's coefficients ranged from 0.55 to 0.95. Cluster analysis was obtained using UPGMA (Unweighted Pair Group Method of Arithmetic Average) algorithm. Maximum no. of genes (23) was amplified from TW-760010 genotype whereas minimum no of genes (14) were amplified from TW-76005 genotype. The data gained from present study open up new ways to produce new varieties by breeding rust resistant germplasm to avoid the economic and food loss and varieties with improved characteristics.
authors.affiliation: Department of Bioinformatics and Biotechnology, Government College University, Faisalabad.
authors.forename: Asma
authors.initials: A
authors.lastname: Haque
authors.affiliation: Department of Bioinformatics and Biotechnology, Government College University, Faisalabad.
authors.forename: Tayyaba
authors.initials: T
authors.lastname: Shaheen
authors.affiliation: Department of Applied Chemistry, Government College University, Faisalabad.
authors.forename: Tahsin
authors.initials: T
authors.lastname: Gulzar
authors.affiliation: Department of Bioinformatics and Biotechnology, Government College University, Faisalabad.
authors.forename: Mahmood Ur
authors.initials: MU
authors.lastname: Rahman
authors.affiliation: Department of Bioinformatics and Biotechnology, Government College University, Faisalabad.
authors.forename: Fatima
authors.initials: F
authors.lastname: Jalal
authors.affiliation: Department of Bioinformatics and Biotechnology, Government College University, Faisalabad.
authors.forename: Summera
authors.initials: S
authors.lastname: Sattar
authors.affiliation: Department of Bioinformatics and Biotechnology, Government College University, Faisalabad.
authors.forename: Beenish
authors.initials: B
authors.lastname: Ehsan
authors.affiliation: Agriculture Biotechnology Research Institute, AARI, Faisalabad.
authors.forename: Zafar
authors.initials: Z
authors.lastname: Iqbal
authors.affiliation: Agriculture Biotechnology Research Institute, AARI, Faisalabad.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Younas
keywords: Wheat; germplasm; leaf rust; stem rust; stripe rust
references.citation: Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269-73
references.pmid: 291943
references.citation: Theor Appl Genet. 2008 May;116(8):1155-66
references.pmid: 18347772
references.citation: Curr Opin Plant Biol. 2005 Aug;8(4):441-9
references.pmid: 15922652
references.citation: Theor Appl Genet. 2006 Oct;113(6):1027-36
references.pmid: 16896713
references.citation: Theor Appl Genet. 1997 Jan;94(1):139-44
references.pmid: 19352756
references.citation: BMC Plant Biol. 2009 Oct 20;9:125
references.pmid: 19840401
references.citation: Phytopathology. 2009 Oct;99(10):1209-15
references.pmid: 19740035
references.citation: J Appl Genet. 2001;42(2):117-26
references.pmid: 14564046
references.citation: Theor Appl Genet. 2007 May;114(8):1379-89
references.pmid: 17356867
references.citation: Theor Appl Genet. 2005 Sep;111(5):846-50
references.pmid: 16025305