title: Technical note: A comparison of DNA collection methods in cattle and yaks.
abstract: A variety of biological materials are suitable for the analysis of bovine DNA. The objective of this study was to evaluate the ease of collection, storage, and cost as well as quality and quantity of DNA samples obtained from Bos taurus (European cattle) and Bos grunniens (yak) using 2 different sample types: whole blood sampling and nasal swabs. Hair follicle DNA samples from yaks were also analyzed. Deoxyribonucleic acid samples were collected from 1 herd of Black Angus yearling bulls (n = 166) and 1 herd of yaks (n = 24). A NanoDrop Bioanalyzer ND1000 was used to quantify DNA. To assess DNA purity, absorbance ratios were determined at wavelengths of 260 nm relative to 280 nm and 260 nm relative to 230 nm. Single nucleotide polymorphism genotyping was performed using a competitive allele-specific PCR (KASP) genotyping system and the call rates to 3 specific SNP were compared. Using a commercially available nonautomated ethanol DNA extraction technique, nasal swabs yielded a greater quantity of DNA than blood (P < 0.0001) and a greater quality DNA sample than blood (P < 0.0001). Blood and nasal swab performance in SNP genotyping assays were similar (P = 0.5). The greater expense of nasal swabs was offset by their ease of use: less time, skill, and equipment was needed to obtain a sample and the storage of samples was more convenient (room temperature). In yaks, accessing the coccygeal vein, which is relatively straightforward in cattle, was difficult. Nasal swabbing and hair follicle sampling in yaks was performed relatively easily. Yak hair follicles were a poor source of DNA. In conclusion, DNA collection using nasal swabs was more convenient and provided a greater quantity of DNA and better quality sample than blood collection in both Angus and yak. Notably, yak hair was a poor source of DNA, and yak blood was difficult to obtain.
authors.affiliation: MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK marianne.neary@googlemail.com.
authors.forename: M T
authors.initials: MT
authors.lastname: Neary
authors.affiliation: Integrated Livestock Management, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
authors.forename: J M
authors.initials: JM
authors.lastname: Neary
authors.affiliation: Integrated Livestock Management, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
authors.forename: G K
authors.initials: GK
authors.lastname: Lund
authors.affiliation: Integrated Livestock Management, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
authors.forename: F B
authors.initials: FB
authors.lastname: Garry
authors.affiliation: Integrated Livestock Management, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
authors.forename: T N
authors.initials: TN
authors.lastname: Holt
authors.affiliation: MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
authors.forename: T J
authors.initials: TJ
authors.lastname: Mohun
authors.affiliation: MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK Division of Medicine, University College London, WC1E 6JJ, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Breckenridge
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood* / Q000235:genetics*; D004247:DNA; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D013045:Species Specificity; D013048:Specimen Handling
chemical_list: D004247:DNA
keywords: blood; cattle; deoxyribonucleic acid collection; hair; nasal swab; yak