title: Fatty acid-specific alterations in leptin, PPARα, and CPT-1 gene expression in the rainbow trout.
abstract: It is known that fatty acids (FA) regulate lipid metabolism by modulating the expression of numerous genes. In order to gain a better understanding of the effect of individual FA on lipid metabolism related genes in rainbow trout (Oncorhynchus mykiss), an in vitro time-course study was implemented where twelve individual FA (butyric 4:0; caprylic 8:0; palmitic (PAM) 16:0; stearic (STA) 18:0; palmitoleic16:1n-7; oleic 18:1n-9; 11-cis-eicosenoic 20:1n-9; linoleic (LNA) 18:2n-6; α-linolenic (ALA) 18:3n-3; eicosapentenoic (EPA) 20:5n-3; docosahexaenoic (DHA) 22:6n-3; arachidonic (ARA) 20:4n-6) were incubated in rainbow trout liver slices. The effect of FA administration over time was evaluated on the expression of leptin, PPARα and CPT-1 (lipid oxidative related genes). Leptin mRNA expression was down regulated by saturated fatty acids (SFA) and LNA, and was up regulated by monounsaturated fatty acids (MUFA) and long chain PUFA, whilst STA and ALA had no effect. PPARα and CPT-1mRNA expression were up regulated by SFA, MUFA, ALA, ARA and DHA; and down regulated by LNA and EPA. These results suggest that there are individual and specific FA induced modifications of leptin, PPARα and CPT-1 gene expression in rainbow trout, and it is envisaged that such results may provide highly valuable information for future practical applications in fish nutrition.
authors.affiliation: Department of Sciences and Technologies, Via Port'Arsa, 11, 82100, Benevento, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Coccia
authors.forename: Ettore
authors.initials: E
authors.lastname: Varricchio
authors.forename: Pasquale
authors.initials: P
authors.lastname: Vito
authors.forename: Giovanni Mario
authors.initials: GM
authors.lastname: Turchini
authors.forename: David Scott
authors.initials: DS
authors.lastname: Francis
authors.forename: Marina
authors.initials: M
authors.lastname: Paolucci
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D029941:Fish Proteins / Q000235:genetics*; D005398:Fisheries; D005786:Gene Expression Regulation / Q000187:drug effects; D066298:In Vitro Techniques; D020738:Leptin / Q000235:genetics*; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000378:metabolism*; D047493:PPAR alpha / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D029941:Fish Proteins; D020738:Leptin; D047493:PPAR alpha; D012333:RNA, Messenger; D002334:Carnitine O-Palmitoyltransferase
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